LOCUS       DAXDXW010000001        63311 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXDXW010000001 DAXDXW010000000
VERSION     DAXDXW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 63311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63311
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.001"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(143..862)
                     /gene="pyrH"
                     /locus_tag="ACFB64_000001"
     CDS             complement(143..862)
                     /gene="pyrH"
                     /locus_tag="ACFB64_000001"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC1208471.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEIVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALNKNLKVMDATAFALCRERKLNIVVFGI
                     AKQGSLKRVITGEDEGTLVHC"
     gene            complement(1079..1933)
                     /gene="tsf"
                     /locus_tag="ACFB64_000002"
     CDS             complement(1079..1933)
                     /gene="tsf"
                     /locus_tag="ACFB64_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC1208472.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVESERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENDTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(2062..2790)
                     /gene="rpsB"
                     /locus_tag="ACFB64_000003"
     CDS             complement(2062..2790)
                     /gene="rpsB"
                     /locus_tag="ACFB64_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC1208473.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQETAAE"
     gene            complement(2963..3196)
                     /locus_tag="ACFB64_000004"
     CDS             complement(2963..3196)
                     /locus_tag="ACFB64_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208474.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWVVLPTVFTVFA
                     VTVRRRNRLMGQQEQAESEQQRAQRQKDSGTKP"
     gene            3274..3855
                     /locus_tag="ACFB64_000005"
     CDS             3274..3855
                     /locus_tag="ACFB64_000005"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC1208475.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAAAVKINRLLKRYIKRGR
                     KIGVYWPMGKELRLGGFVRAAQKRGAKLYLPYIEPHTRRMWFTPYPERGMERERKRGR
                     AKLHVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLSAMKYRLQAKTV
                     GVGFACQLVDRLPREAHDLPLDGFVSEAGILCF"
     gene            3916..4506
                     /locus_tag="ACFB64_000006"
     CDS             3916..4506
                     /locus_tag="ACFB64_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFC1208476.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEADRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRKRINTCCKIMLLDYAFDVLACCCAGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            4890..6356
                     /locus_tag="ACFB64_000007"
     CDS             4890..6356
                     /locus_tag="ACFB64_000007"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC1208477.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGADGVINPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCRYLQKRYDVFKTQKLFENMEFSTDRNKISDWAPLIMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDWQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGLPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSFMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(6543..6914)
                     /locus_tag="ACFB64_000008"
     CDS             complement(6543..6914)
                     /locus_tag="ACFB64_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC1208478.1"
                     /translation="MKLLTTAILSSAIALSSMAAAGTDNPTVAKKTVSYVCQQGKKVK
                     VTYGFNKQGLTTYASAVINGKRVQMPINLDKSDNMDTFYGKEGGYVLSTGAMDSKSYR
                     KQPIMITAPDNQIVFKDCSPR"
     gene            complement(7152..7451)
                     /locus_tag="ACFB64_000009"
     CDS             complement(7152..7451)
                     /locus_tag="ACFB64_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1208479.1"
                     /translation="MKIFENIEDVKAIRKKTGMNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECLDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKAERKKQG"
     gene            complement(7550..8329)
                     /gene="map"
                     /locus_tag="ACFB64_000010"
     CDS             complement(7550..8329)
                     /gene="map"
                     /locus_tag="ACFB64_000010"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC1208480.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGITTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGGVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPATGKP"
     gene            complement(8369..9019)
                     /locus_tag="ACFB64_000011"
     CDS             complement(8369..9019)
                     /locus_tag="ACFB64_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208481.1"
                     /translation="MPFEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAVGIVSTFNPNGDKTLQTEPQHTDSPRETEFWLPNGAVGQDAAQPEHHHAAS
                     SEPAQPDGTEESGSGLPPPAAPKKNRVKPRPSDAARAADSLTGTGTQAENTLKETPVL
                     PTNAPHPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPAKPHKEILDNLF"
     gene            complement(9027..9635)
                     /locus_tag="ACFB64_000012"
     CDS             complement(9027..9635)
                     /locus_tag="ACFB64_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC1208482.1"
                     /translation="MKPKPHTVRTLIAAVLSLALGGCFSAVVGGAAVGAKSVIDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIIT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            complement(9699..10292)
                     /locus_tag="ACFB64_000013"
     CDS             complement(9699..10292)
                     /locus_tag="ACFB64_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC1208483.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKILVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            complement(10297..10644)
                     /locus_tag="ACFB64_000014"
     CDS             complement(10297..10644)
                     /locus_tag="ACFB64_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC1208484.1"
                     /translation="MRLNHKQGTAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNQRFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIKNITG"
     gene            10691..11566
                     /gene="rsmI"
                     /locus_tag="ACFB64_000015"
     CDS             10691..11566
                     /gene="rsmI"
                     /locus_tag="ACFB64_000015"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC1208485.1"
                     /translation="MFQKHLQKAPDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGRLVSVREHNERQMADKVIGFLSDGLVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVAESDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALAADGNQSRGEMVLVLYPAQDEKHEGLSESAQNAMKILAAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            complement(11601..12131)
                     /locus_tag="ACFB64_000016"
     CDS             complement(11601..12131)
                     /locus_tag="ACFB64_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFC1208486.1"
                     /translation="MNTNQPAVYDPLTRALHWLTVAGFIGILTTIVLWTIYEEAEWAG
                     SLFGLHKSFGFLMLTVIALRIVWAVANRAKRPQSDSKAAAAGHGILYLLMLAVPVIGM
                     IRQYGGGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLFAAVVGHVAMVVVH
                     RVQGKDVLYRMTGRVR"
     gene            12410..13564
                     /gene="obgE"
                     /locus_tag="ACFB64_000017"
     CDS             12410..13564
                     /gene="obgE"
                     /locus_tag="ACFB64_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC1208487.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPEPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLAEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            13618..14565
                     /locus_tag="ACFB64_000018"
     CDS             13618..14565
                     /locus_tag="ACFB64_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1208488.1"
                     /translation="MYKTIDLFSGIGGIRLGFEKYGCTNVFSSEWDKYARQVYEANFG
                     EKPFGDINGIDPSDIPDHDILLAGFPCQPFSIAGKGLGFEDTRGTLFFNIAEILKTKQ
                     PKAFLLENVKRLTTHDSGRTFRIVLETLKQLGYTVYFKVLNTLDFGLPQKRERIYIVG
                     FSDNIPFYFPEPINQYRPLGELLENDRDVEPSYFLSDTLKQKRLAALKKAPPTPSIWH
                     ENIGGNVSALPYSCALRAGGSYNYLVVNGVRRLTGREMLRLQGFPDDFEINIPYSQVR
                     KVAGNSVSVPVIEAIAENMLASLSGKVEQKGQLDLLEAG"
     gene            14562..15614
                     /locus_tag="ACFB64_000019"
     CDS             14562..15614
                     /locus_tag="ACFB64_000019"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HaeII family restriction endonuclease"
                     /protein_id="HFC1208489.1"
                     /translation="MTLEEQQAKEALDGIIKKSRVHLYKPIQIAEILYHDRCIKQLDF
                     LNLDTYRNQSKRWRDEICRRFLGRISTSSAKFQDNLFEKNAIPPEKLAVLGTLNRQSD
                     GGVESYIYKQFFNRFSQMSEALAYVGNTDRYSFQLSEFLNLFWLEPGLKRSIDKIYEI
                     VVYALFDALVSELGITVSIDFPKENLFLWEEYQDFAEKIITMPKNEHLKLPAKIHRVG
                     VTNAADRGLDMWSNFGLAIQVKHLSLDEELAEDIVSSISADRIVIVCKKAEQSVIVSL
                     LTQIGWKSRIQNIVTEDDLISWYEKALRGQYPIAEALLENIKTEIMREFPAVNEANEF
                     LDFAQNRGYDITVTHF"
     gene            15630..17051
                     /gene="cysS"
                     /locus_tag="ACFB64_000020"
     CDS             15630..17051
                     /gene="cysS"
                     /locus_tag="ACFB64_000020"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC1208490.1"
                     /translation="MTAIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELNARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAHL
                     AGCLKALGGIIGLLQRNPIEFLQGGAVSDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDSAGGTTWRRG"
     gene            17222..18001
                     /locus_tag="ACFB64_000021"
     CDS             17222..18001
                     /locus_tag="ACFB64_000021"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC1208491.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGYWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LGVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHTLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            17988..18332
                     /locus_tag="ACFB64_000022"
     CDS             17988..18332
                     /locus_tag="ACFB64_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208492.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAVFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIAAKCLGWKGWRRVVNIFVCLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            18342..18872
                     /locus_tag="ACFB64_000023"
     CDS             18342..18872
                     /locus_tag="ACFB64_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC1208493.1"
                     /translation="MESFELEDLTLWLIRDADEAEMWIDRWAVSYPVVQMSEASANGS
                     TEAWQSALQTAFERIQGRHIAVVAHGAGAAAFLAWLYRADILTQKKLAGIILVSPRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(18965..20080)
                     /gene="asd"
                     /locus_tag="ACFB64_000024"
     CDS             complement(18965..20080)
                     /gene="asd"
                     /locus_tag="ACFB64_000024"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC1208494.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTSSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIALDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAIHAQVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSGNPTVIDGLCVRIGAMRCHS
                     QAITLKLKKDLPVSEIEAILAGANDWVKVVPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPMRRVLRIVLGSL"
     gene            20255..20836
                     /locus_tag="ACFB64_000025"
     CDS             20255..20836
                     /locus_tag="ACFB64_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC1208495.1"
                     /translation="MEEKNDYTDAVSDNRNGQEIEVGIAGAGDRILAALLNQLFTFLI
                     LLVPFVGLIAFAVKNEGRIGGREEIFGLLLGMTSFWVGLAGILAYTVIQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGENGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(20898..21752)
                     /gene="folD"
                     /locus_tag="ACFB64_000026"
     CDS             complement(20898..21752)
                     /gene="folD"
                     /locus_tag="ACFB64_000026"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFC1208496.1"
                     /translation="MSAQLINGKEVSQKHLQAIAEAVAQRQQDNLHTPCLAVVLVGGD
                     PAGAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAILERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAAADILVVGVGIPNFVKG
                     GWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            21975..22052
                     /locus_tag="ACFB64_000027"
     tRNA            21975..22052
                     /locus_tag="ACFB64_000027"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22010..22012,aa:Pro,seq:tgg)
     gene            22077..22153
                     /locus_tag="ACFB64_000028"
     tRNA            22077..22153
                     /locus_tag="ACFB64_000028"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22111..22113,aa:Arg,seq:tct)
     gene            22181..22256
                     /locus_tag="ACFB64_000029"
     tRNA            22181..22256
                     /locus_tag="ACFB64_000029"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22214..22216,aa:His,seq:gtg)
     gene            22468..22974
                     /gene="rfaE2"
                     /locus_tag="ACFB64_000030"
     CDS             22468..22974
                     /gene="rfaE2"
                     /locus_tag="ACFB64_000030"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HFC1208497.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKGGDRPVNSLENRAAVAAALESVDLVTWF
                     DEDTPAALIEAVKPEVLVKGGDWVVDKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            22971..24749
                     /locus_tag="ACFB64_000031"
     CDS             22971..24749
                     /locus_tag="ACFB64_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC1208498.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLAREADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRDLGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWAFDRPQYELGSLSPVAA
                     LACRRALGCLGLETQIKWPNDLVVGRDKLGGILIETVRAGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELGAVLEQYAEEGFAPFLNEYETANRD
                     HGKAVLLLRDGETVCEGTVKGVDGRGVLHLETAEGEQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGESKKAQVKEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRPAGKRYPFPTTT
                     GNAVASGMMDAVCGSIMMMHGRLKEKNGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHA"
     gene            24862..25686
                     /locus_tag="ACFB64_000032"
     CDS             24862..25686
                     /locus_tag="ACFB64_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC1208499.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKAAGGVPENRAVENTPPA
                     TPAAGNAAASVEDTAALLKPGDILSEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPVPFDGALSLGVGNSRENAQALQNRLADAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(26192..26404)
                     /locus_tag="ACFB64_000033"
     CDS             complement(26192..26404)
                     /locus_tag="ACFB64_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HFC1208500.1"
                     /translation="MPSEGFRRHRGPKRANRHSPASRLCREEQGNRPENLAARPDTAT
                     NETPGLPGAQAAAQALSTAIEGSFVV"
     gene            complement(26419..27207)
                     /locus_tag="ACFB64_000034"
     CDS             complement(26419..27207)
                     /locus_tag="ACFB64_000034"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC1208501.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     TGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALKILRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARTKAQASTPTVGQPFWHSAEY"
     gene            complement(27286..27480)
                     /gene="thiS"
                     /locus_tag="ACFB64_000035"
     CDS             complement(27286..27480)
                     /gene="thiS"
                     /locus_tag="ACFB64_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFC1208502.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFIPKGAY
                     TETVLHENDKIDIVRPVVGG"
     gene            complement(27902..28519)
                     /gene="thiE"
                     /locus_tag="ACFB64_000036"
     CDS             complement(27902..28519)
                     /gene="thiE"
                     /locus_tag="ACFB64_000036"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC1208503.1"
                     /translation="MTFPPLKSLLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGNE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPGYIASGAIFQTTTKQMPTAPQGLDKLREYVEQARGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
     gene            complement(28541..29641)
                     /locus_tag="ACFB64_000037"
     CDS             complement(28541..29641)
                     /locus_tag="ACFB64_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC1208504.1"
                     /translation="MTRIAVLGGGLSGRLTALQLAEQGYQIELFDKGTRQGEHAAAYV
                     AAAMLAPAAEAVEATPEVIRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPQDLQAQYDWVIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIAAGLRPTLNHHNPEIRYSRERRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIGRQD"
     gene            complement(29638..30864)
                     /gene="cytX"
                     /locus_tag="ACFB64_000038"
     CDS             complement(29638..30864)
                     /gene="cytX"
                     /locus_tag="ACFB64_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC1208505.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKCGSVLFSVANMLQLAGWTAVM
                     IYVGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARRTGGLKTVSMLLMLLAV
                     LWLSVEVFASSGTNAAPAVSDGMTFGTAVKLSAVMPLSWLPLAADYTRQARRPFAATL
                     TATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGITGILAVVLSTVTTTFLDT
                     YSAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLI
                     ADFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVS
                     VRLFFKKTQSLQRNPS"
     regulatory      complement(30930..31034)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            31109..32593
                     /gene="tldD"
                     /locus_tag="ACFB64_000039"
     CDS             31109..32593
                     /gene="tldD"
                     /locus_tag="ACFB64_000039"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC1208506.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQIDQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGGA
                     GGGGRYDLAYFDETLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRQSYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFGASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     gene            32737..33396
                     /locus_tag="ACFB64_000040"
     CDS             32737..33396
                     /locus_tag="ACFB64_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC1208507.1"
                     /translation="MNWPYLIDAVPKFADAAKLTLELSVYGVVLSLLFGLPVAVVTAY
                     RIRPFYALARAYIELSRNTPLLIQLFFLYYGLPKMGIKWDGFTCGVIALVFLGASYMA
                     EAVRAGILAVPKGQVGAGKAIGLSRFQVFRYVELPQVWAVAVPAIGANILFLMKETSV
                     VSTVGIAELLFVTKDVIGMDYKTNEALFLLFAAYLIILLPVSLLARRIENRVRSAKYG
                     V"
     gene            33386..34054
                     /locus_tag="ACFB64_000041"
     CDS             33386..34054
                     /locus_tag="ACFB64_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC1208508.1"
                     /translation="MAFEWLFEGKNAARLGEGLLLTAQISLISVAASCVLGTLFGLVL
                     RSRNRLVRFVGRFYLETIRIVPILVWLFGLYFGLSVWTGIHIGGFWVCVWVFSLWGVA
                     EMGDLVRGALESIEKHQVESGLAPGLSRGQVFRCIELPQSIRRVLPGAVNLFTRMIKT
                     SSLAWLIGVIEVVKVGQQIIENSLLTQPNASFWVYGLIFMLYFFCCWPLSLLAAKLEQ
                     KWEH"
     gene            34056..34787
                     /locus_tag="ACFB64_000042"
     CDS             34056..34787
                     /locus_tag="ACFB64_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC1208509.1"
                     /translation="MALLSIRKLHKQYGSVTAIQSLDLDLEKGEVIVLLGPSGCGKST
                     LLRCVNGLEPHQGGSIVMDGVGEFGKDVSWQTARQKVGMVFQSYELFAHMTVIENILL
                     GPVKVQNRDRAEAEAQAGKLLERVGLLDRKNAYPRELSGGQKQRIAIVRALCLNPEVI
                     LLDEITAALDPEMVREVLEVVLELAREGMSMLIVTHEMGFARKVADRIVFMDKGGIVE
                     SSDPETFFSAPKSERARQFLAGMDY"
     gene            34900..35754
                     /locus_tag="ACFB64_000043"
     CDS             34900..35754
                     /locus_tag="ACFB64_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC1208510.1"
                     /translation="MKLNAKLKALLASAAIAVGLTACGGGSGDAQSSQSSGAATVAAI
                     KEKGVIRIGVFGDKPPFGYVDANGKNQGFDVEIAKDLAKDLLGSPDKVEFVLTEAANR
                     VEYVRSGKVDLILANFTQTPERAEAVDFADPYMKVALGVVSPKNKPITDMAQLKDQTL
                     LVNKGTAADAFFTKSHPEVKLLKFDQNTETFDALKDGRGVALAHDNALLWAWAKENPN
                     FEVAIGNLGPAEFIAPAVQKGNADLLNWVNGEIAAMKKDGRLKAAYEKTLLPVYGEKV
                     KPEALLAE"
     gene            36170..36991
                     /gene="prmC"
                     /locus_tag="ACFB64_000044"
     CDS             36170..36991
                     /gene="prmC"
                     /locus_tag="ACFB64_000044"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC1208511.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEI
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPNVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSERQWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVEILPDLAGLDRVTLGKYMKHLK"
     gene            37077..38465
                     /locus_tag="ACFB64_000045"
     CDS             37077..38465
                     /locus_tag="ACFB64_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC1208512.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNVIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLVAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCTGL
                     GFSPLATAAIVGTAGALGDAGSPASDSTLGPTMGLNADGRHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            38466..38690
                     /locus_tag="ACFB64_000046"
     CDS             38466..38690
                     /locus_tag="ACFB64_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC1208513.1"
                     /translation="MDAVQELERRIVELEIQTALQEDVISGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(39090..39983)
                     /locus_tag="ACFB64_000047"
     CDS             complement(39090..39983)
                     /locus_tag="ACFB64_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC1208514.1"
                     /translation="MPHPCQTVHTLTTHVPKCRLKLNAARRRRYNRPQLFFPNIIMTA
                     TEHDNDDALLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAETLAGRLKRINHTVDVRAVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAAYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVLSPLVGIVGCTQAAEALKILLEAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGAAR"
     gene            40026..42728
                     /gene="ppc"
                     /locus_tag="ACFB64_000048"
     CDS             40026..42728
                     /gene="ppc"
                     /locus_tag="ACFB64_000048"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC1208515.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDERQTYDLTLACGLFAQILNIAEDVHHERRRQIHEDAGHNA
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLNFNRRIRALL
                     PQRERCTNADALARLRREIDTVLLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSGETLRFAFRRHADAVFR
                     FYRSELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARSLGLGMGCKFGFLEPYASAQKFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELGHQRPLYSP
                     FITYSDHTRRELAIFNEARKIKDEFGEDAVTQSIISNCEQPGDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLHQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEGSPETLAALRGHAQNNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAKVIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            42851..43840
                     /locus_tag="ACFB64_000049"
     CDS             42851..43840
                     /locus_tag="ACFB64_000049"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC1208516.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLAEALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGSVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            43894..44223
                     /locus_tag="ACFB64_000050"
     CDS             43894..44223
                     /locus_tag="ACFB64_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208517.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELERAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENARYRALLEQSREKISALAARLP
                     QRQETQQ"
     gene            44234..44539
                     /locus_tag="ACFB64_000051"
     CDS             44234..44539
                     /locus_tag="ACFB64_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC1208518.1"
                     /translation="MNIEQVYIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKITDMDNACQKALSRLGQE"
     gene            44545..44724
                     /gene="ssrS"
                     /locus_tag="ACFB64_000052"
     ncRNA           44545..44724
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFB64_000052"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            44902..45333
                     /gene="rplM"
                     /locus_tag="ACFB64_000053"
     CDS             44902..45333
                     /gene="rplM"
                     /locus_tag="ACFB64_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC1208519.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVATEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQDQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            45346..45738
                     /gene="rpsI"
                     /locus_tag="ACFB64_000054"
     CDS             45346..45738
                     /gene="rpsI"
                     /locus_tag="ACFB64_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC1208520.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLTKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESLDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(46119..46613)
                     /locus_tag="ACFB64_000055"
     CDS             complement(46119..46613)
                     /locus_tag="ACFB64_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208521.1"
                     /translation="MRLTKYPYNSIRELEDYLINTYQKYIILQEGGKEIYRCFILSFY
                     KEFNIGIGLAVSCISIPPKVLMLDDKNIFIGFDSVVFCISIQNSKVNILNIDGIVFDI
                     YLLDNQKICIIHELGAIITDKNLIRENSVSTDIISDWEIDKVNKLIILKGLDSEKIIS
                     LNYD"
     gene            complement(46806..47735)
                     /locus_tag="ACFB64_000056"
     CDS             complement(46806..47735)
                     /locus_tag="ACFB64_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC1208522.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQSSIS
                     FHPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            47864..48613
                     /locus_tag="ACFB64_000057"
     CDS             47864..48613
                     /locus_tag="ACFB64_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC1208523.1"
                     /translation="MVLRRDFLAWCDETLQTASFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     VHPILGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVAI
                     GNPEREIRRVAWCTGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            48736..49317
                     /gene="petA"
                     /locus_tag="ACFB64_000058"
     CDS             48736..49317
                     /gene="petA"
                     /locus_tag="ACFB64_000058"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC1208524.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            49336..50685
                     /locus_tag="ACFB64_000059"
     CDS             49336..50685
                     /locus_tag="ACFB64_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC1208525.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            50700..51488
                     /locus_tag="ACFB64_000060"
     CDS             50700..51488
                     /locus_tag="ACFB64_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC1208526.1"
                     /translation="MKQAMKNWFAALLSAVPMSAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDTYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLLAVMLPLAYFLKKEYWKDVH"
     gene            51820..53610
                     /gene="rmuC"
                     /locus_tag="ACFB64_000061"
     CDS             51820..53610
                     /gene="rmuC"
                     /locus_tag="ACFB64_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC1208527.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAQLAEKAA
                     RCDFIEQAHAEIASELAALDGKYEHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIDLQEKAQGLAVENERLATQLGQERKAFAEQYALERQIRQRVETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAYLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVRQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTVYVRYTQAADADEAARELAAYIASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERANEQTAFALGKPSEQEAADEAE"
     gene            complement(53677..54309)
                     /locus_tag="ACFB64_000062"
     CDS             complement(53677..54309)
                     /locus_tag="ACFB64_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC1208528.1"
                     /translation="MTLRYEILPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLETHKVPVLGPHREDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFAFPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            54402..55226
                     /locus_tag="ACFB64_000063"
     CDS             54402..55226
                     /locus_tag="ACFB64_000063"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC1208529.1"
                     /translation="MIKKIIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRRHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            55295..55858
                     /locus_tag="ACFB64_000064"
     CDS             55295..55858
                     /locus_tag="ACFB64_000064"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC1208530.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGD
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKEKPVKADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALGGK"
     gene            55942..56379
                     /locus_tag="ACFB64_000065"
     CDS             55942..56379
                     /locus_tag="ACFB64_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC1208531.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEGQD
                     DIINNAISALQEFPENHGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMIKAV
                     QYSPAAEDFAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            56600..56869
                     /locus_tag="ACFB64_000066"
     CDS             56600..56869
                     /locus_tag="ACFB64_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC1208532.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFKSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGLDEAAAMKALTDLINDYFGEGE"
     gene            56869..58644
                     /gene="ptsP"
                     /locus_tag="ACFB64_000067"
     CDS             56869..58644
                     /gene="ptsP"
                     /locus_tag="ACFB64_000067"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC1208533.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAQADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIISPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGEVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPSVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNAGQLEGDIAKVIRCEDE
                     EKSEKLIKQINSVSVAEEAEVKGRK"
     gene            complement(58771..59712)
                     /locus_tag="ACFB64_000068"
     CDS             complement(58771..59712)
                     /locus_tag="ACFB64_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC1208534.1"
                     /translation="MLELNGLCKCFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIRLNGENITCMPPEKRRISLMFQDYALFPHMSALENTAFGLKMQK
                     MPKAEAERLALSALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LIQTPAGVQVARLMGLPNTDDDRHIPQNAVCLDNHGTECRLLSLVRLPDSLRLSAVHP
                     EHGELTLNLTVGQHTDGISGNGTVRIRVDEGRIVRFR"
     gene            complement(59721..60596)
                     /locus_tag="ACFB64_000069"
     CDS             complement(59721..60596)
                     /locus_tag="ACFB64_000069"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC1208535.1"
                     /translation="MPTVRFTESVSKQDLDALFERAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERIKKDWEAGCSESSDGIFLNADGWPDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLVESNGRWHFWIGRRSPHKAVDPGK
                     LDNIAGGGVSGGEMPSEAVCRESSEEAGLDKTLFPLIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVAGFEKMDIGGLLDAMLSKNMMHDAQLVTLDAFYRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(60779..60855)
                     /locus_tag="ACFB64_000070"
     tRNA            complement(60779..60855)
                     /locus_tag="ACFB64_000070"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(60819..60821),aa:Met,seq:cat)
     regulatory      61004..61103
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            61194..63095
                     /gene="thiC"
                     /locus_tag="ACFB64_000071"
     CDS             61194..63095
                     /gene="thiC"
                     /locus_tag="ACFB64_000071"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC1208536.1"
                     /translation="MTTPKKTAKTSGNEARELSDLSEDIGIRFKYQNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGAYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFAAIRERMKLDELFRRPEYAKLLKQHTGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQTLEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT        14514 a        16566 c        17843 g        14388 t
ORIGIN      
        1 agtggattaa atttaaaccg gtacggcaag gcgaggcaac gccgtaccgg tttaaattta
       61 atccactata atctccatat aataaatggc tgtctgaagc ccatatgcga ctatatctgc
      121 cgaccctatg gtcaatcatc aatcagcagt ggaccagtgt gccttcgtct tcgccggtaa
      181 tgacgcgttt gagcgagcct tgtttggcga tgccgaagac gacgatattg agcttgcgtt
      241 cgcggcagag ggcaaaggcg gtcgcgtcca tgactttgag gtttttattc aaggcttcgt
      301 caaaagtaat ggtttcgtag cgtgtggcgg acgggtcttt tttcgggtct gcggtgtaca
      361 cgccgtcgac gttggtggct ttgagcatca cgtcgcagtt catttccgcg ccgcgcaatg
      421 cggcggcagt gtcggtcgtg aagaacggat taccggtacc ggcggcgaaa atcacgactt
      481 tgccttcttc caaatattga atggctttag gacgggcgta agtttcagcg atttgctgca
      541 tagacagtgc ggattgtacg cgcgctttga tgcccaaggt ttcaaatgcg tctttgagtg
      601 ccaaggcgtt catcacggtc gccatcatgc ccatgtagtc ggcagtggcg cggtccatgc
      661 tgcctgcttg tgcagatacg ccgcggaaaa tgttaccgcc gccgacaacg ataccgactt
      721 gcacacccat tttcacgatt tcggcaattt cgccgacagt ttgaacaatg gtatcgtgat
      781 tgatgccgaa cggatcggaa cccatcaggg attcgccgga gagtttcagt aatacgcgtt
      841 tgtatttgat ttgctgtgtc atgggatacc ttgctttctt gaatgtcgtc ggtcggagtt
      901 gggacgtctg aatttcaaat ggtcggatta caatgatatg gtgtgtcgtt ctattattgt
      961 tttgttttca gacggccttt atattcggtc gtcttaaaat aggtaccgta aacggttttc
     1021 tcaaaaaaaa gcaccctgat tcgtttggaa tccaggtgct ttctttttca taagtgcctt
     1081 acactttggc ggctgcggct acttcggctg cgtagtcgac aacggctttc tcgataccgt
     1141 cgcctacttt gtagcgtacg aagctaacca cttcagtgtc gttttcttta gcgaattggg
     1201 caacagtttg gtcagggttc atcacgaatg cttggccgtt cagagtgatt tcagccaaga
     1261 atttgcggat acggccttca accattttag cggcgatgtc ggcaggtttg ccggaagcga
     1321 tggcttgctc ggtgtagatg tggcgttctt tttcaacggt ttcagcatct acttcggctt
     1381 cgcttacgca ttgcggtttg gcggcaacga tgtgcatacc gattttgcgt gctacatctt
     1441 cagagccttt gtactcgacc aaaacgcctt cggtcgccaa tgcgccgtgg atgtaggcaa
     1501 ccagttggtt ggcagtgtca atcacttgga agcggcggac agacatgttc tcgcccaatt
     1561 tagcgatgat ggctttgcgt tctgattcaa ccagttcgct cagttcttca acagaagccg
     1621 gttttttctc ggcagcagtt ttcgcaacga agttggcaaa ttctacgaag ccggcgtctt
     1681 tagcaacgaa gtcggtttcg cagtttactt cgaccaatgc gccgacatta ccgttgattg
     1741 cataagccaa tacgccttcg gcagcagtac ggccagccag tttgcccgct ttcgcaccgg
     1801 atttaatacg caggatttct tcggctttgt cgaagttgcc ttcagcttca accaaggctt
     1861 ttttgcattc catcataccc aggccggtag cggcgcgcag gtcggcaacc atttttgcag
     1921 taatttctgc cattttgaat ctcctagatt tttgggaaat acggaaagga ttttccacat
     1981 tcgggaatcg tgtgccgcga acgggcgttc agacggcata tttgagaaaa ggggcataac
     2041 gcccctcttc tgtgtaccga attactcggc agcggtttct tgggcagcgg ctacggtttc
     2101 ttgcagcgct tggtttttgc cttccaaaac tgcgtcggcg atgcctcggc agtacaggcg
     2161 gatggctttg gcggagtcgt cgttgccggg gatgacgtat ttcacgccgt ctgggctgtt
     2221 gttggtatcg actacggcga taacggggat gcccagtttt tcagcctcaa ccaaagtacc
     2281 tttttggtag ccggtatcga taacgaaaat cgcgtcgggc aggcctttca tgtttttgat
     2341 accgcccaaa gagcgttcca gtttttcaac gtcgcgttgc atttccagaa tttctttttt
     2401 gctgaaaccg ctttcggcag cattttccaa ggctgcggtt ttttcttcca ggcgtttgat
     2461 ggattgctta acggttttgt agttggtcag cataccgccc aaccagcggt ggtcgacgaa
     2521 aggcataccg gcgcgggtcg cttcttcgcg gatgatgtcg cgtgcctggc gtttggtacc
     2581 tacgaacaat accgtacctt tgttggcaac cagacgacgt acggcttctt gcgcctcttg
     2641 gaacatcggc agggtttttt ccaagtttac gatatggatt ttgttgcgcg cgccgaaaat
     2701 gtattgagcc attttcgggt tccagaaacg ggtttggtga ccgaagtgaa caccggcttc
     2761 aatcatctga cgcatagtaa tttgagacat gttatttcct tgaaagggtt aaagagcaca
     2821 cattcaatcg catagaactt gataggcggc gcctattgtc aagcaccctt cggcgaaagt
     2881 gggcataagt tttaaaaata gtgtgtttcc gaaatggaaa actgacggat tatagcggat
     2941 aagatgccgt ctgaaaaggg attcagggtt ttgtgccgct gtctttttgc cgttgtgcgc
     3001 gctgctgttc ggattctgcc tgttcctgct gccccatcag gcggttgcgg cggcggacgg
     3061 ttacggcgaa gaccgtgaac acggtgggca gaaccaccca aaaaaccaaa taaatcaaag
     3121 cgcgcgcaat actcggctgc gcggcggaat acataacggc aacaaaaaga tagccgatgg
     3181 cgacgatgtg ccacattgtg tgcgtcctgt ttgaatgtta acaaaatcgt tataatcgca
     3241 acattctacc gcaatcctga aaggccgtct gaaatgagga acgaggaaaa acgcgccctg
     3301 cgccgcgaat tgcgcgggcg gcgttcgcaa atggggcggg acgtgcgggc ggcggcggcg
     3361 gtaaaaatca accgcctgct caaacgttat atcaagcgcg gtcggaaaat cggcgtgtat
     3421 tggccgatgg gcaaggaatt gcgtttgggc ggctttgtcc gcgcggcgca aaaacgcggc
     3481 gcaaaactct atctgcctta tatcgaaccg cacacgcggc ggatgtggtt tacgccgtat
     3541 cctgaacgcg gaatggaacg ggaacgcaag cgcggtaggg cgaagctgca tgtccctcag
     3601 tttgcagggc gcaaaatccg cgtgcacggt ttgtcggtat tgctcgtccc gcttgtcggc
     3661 atagaccgcg aaggctaccg tttggggcag gcaggcggct attacgatgc gacgctttcg
     3721 gcgatgaaat accgtttgca ggcgaaaacc gtgggcgtgg gctttgcctg ccagttggtg
     3781 gacaggctcc cacgcgaggc gcacgacctg ccgctggacg gttttgtatc ggaagcgggg
     3841 atattgtgtt tttagccgtt cagacggcat ttcgggcgga aatgccgtct gaaggcggaa
     3901 tattgggaga aaaggatgtc ggaaaggata atactgccgg ttttgtcatt gggcggcgta
     3961 cggctcgaac cgttggatgt gcatcatgaa acgggtttgc gcgaggcggt ttgcgatggg
     4021 gaggtttgga agctgggggt aacgtccgcg ccccatccgg atagggtggc agactatatc
     4081 gggacagcat tggcaacgcg tttggcgttt gcggttgtcg atgaagaggc ggacagggtg
     4141 gtcgggacaa cggcgtatta tcattttgaa ccgcagattc cgcgtttgga tatcggattc
     4201 acatggtatg cggcatcggc acggcggaaa cggataaaca cctgttgcaa aatcatgctg
     4261 ttggattacg catttgatgt tttagcttgc tgttgcgcgg gatggcgcac ggatattctg
     4321 aaccttgcct cgcaacgtgc catcgaacgg ctgggcgcgg aaaaagacgg cgtgctgcgt
     4381 atgcatatgc tccgtaaaga cggcagtgtg cgcgatacgg ttgtgtacag tatgctgagg
     4441 gaagattggt gcaaaaacag ggaaatcctg accggcaggt tggcagggta tggcgtgcag
     4501 gtgtgaggca gtattctcaa atttaaaaaa cttaacgcga tatgctgaca atcttttaaa
     4561 aattaatata taaacaattt tgattttttg tctattaaaa atttgatata tatagataaa
     4621 taactatttt tattttttgt ttcgcagttc cccggcttgg cgggcgacag aaaaactttg
     4681 catttcccag taaaatccac tacattcacg ataacatatt acaagcccct gaagcggtgt
     4741 cggaagccat acttgcaacg cattccaacc ccgaaacagc gtgctcggac acacaccatt
     4801 gccggtttcc cctgcttttg cgattcaggc gtttgtagta cggcaaataa ccataagggc
     4861 agaagatgcc tgttttttag gagtaaacca tggctgaagc gacagacgtt gtcttggtgg
     4921 gcggcggcat tatgagcgcg actttgggcg ttttgctcaa agaactcgaa ccgtcttggg
     4981 aaatcaccct gattgaacgc ttggaagatg tggcgttgga atcgtcaaac gcgtggaaca
     5041 acgccggcac ggggcattcc gcgctgtgcg aattgaacta tgcgccgctg ggtgcggacg
     5101 gcgtcatcaa tccggcgcgc gccctcaata ttgccgaaca gtttcatgtc agccgccagt
     5161 tttgggcgac gctggtcgcg gaaggcaagt tggaagacaa ttccttcatc aatgccgtgc
     5221 cgcatatgtc tttggtgatg aacgaagacc actgccgtta cctgcaaaaa cgctatgatg
     5281 tgtttaaaac gcagaaactt tttgaaaata tggaattttc caccgatcgg aacaaaattt
     5341 ccgattgggc tccgctgatt atgcgcggcc gggacgaaaa ccaacccgtc gccgccaact
     5401 attccgccga aggcacggat gtcgatttcg gacggctgac gcgccagatg gtgaaatatt
     5461 tgcagggcaa gggcgtaaaa accgagttca accgccacgt cgaagacatc aaacgcgaat
     5521 ccgacggcgc gtgggtgctc aaaaccgccg atacccgcaa cccagactgg cagctcaccc
     5581 tccgcacccg cttcctcttc ctcggcgcgg gcggcggcgc actgaccctg ctgcaaaaat
     5641 ccggcatccc cgaaggcaaa ggctacggcg gcttacccgt gtccggcctg ttcttccgca
     5701 acagcaaccc cgaaaccgcc gaacaacaca acgccaaagt gtacgggcag gcttccgtcg
     5761 gcgcgccgcc gatgtccgtc ccgcacctcg acacacgcaa cgtagacggc aaacgacacc
     5821 ttatgttcgg tccttacgca ggtttccgtt ccaacttcct caagcaaggc tcgtttatgg
     5881 atttgccgct gtccatccat atggacaacc tctatcctat gctgcgcgcc ggctgggcga
     5941 atatgccgct gaccaaatac ctgctgggcg aattgcgtaa aaccaaagaa gaacgctttg
     6001 cctccctgct ggaatactac cccgaggcaa accccgacga ctgggaactc atcaccgcag
     6061 ggcaacgcgt ccaaatcatt aaaaaagact ccgaaaaagg cggcgtgctg cagtttggta
     6121 cggagattgt cgcccacgcc gacggctcgc tcgccgcatt gctgggagcg tcgccgggcg
     6181 catcgaccgc tgtgccgctg atgatccggc tgatgcacca atgcttcccc gagcgcgccc
     6241 cgtcttggga aggtcgtctg aaagagctgg taccgggtta cggcatcaag ttgaacgaaa
     6301 accctgaaag ggcggatgaa attatcgcct ataccgcgaa agtgttggat atttaaaccg
     6361 aatctttcaa gaaaagccgt acccccatat ggtgttgacg ccgtccctgc cggttatgcc
     6421 cttcatccgg cagggcaggt tttgatacac ggggcaggta atcgtttttt cccttttgcg
     6481 aataatggaa cggtcaaaaa acagcgtttt cagaaatgaa aacgctgttt tttgttgcct
     6541 gattaacgtg gggaacagtc tttgaagacg atttggttgt caggtgcggt aatcataata
     6601 ggctgtttgc ggtaggattt gctgtccatt gcgccggtgc tcagcacata accgccttct
     6661 ttgccgtaga acgtgtccat attgtcggat ttatccaaat tgatgggcat ttgcacacgt
     6721 ttgccgttga tgacggcgga ggcgtatgtg gtcagaccct gtttgttgaa gccgtaggtt
     6781 actttgactt ttttaccttg ctggcagacg tagctgacgg tttttttggc aacggtgggg
     6841 ttgtccgtgc cggcggcggc catactgctg agcgcgattg cggaagacag gattgcagtg
     6901 gtcagaagtt tcatttttat tcctttggat agatgtggag tttgcccgtt tcggactgtt
     6961 tgacagtcta atgtcggcgt atcctgaaat aaacaggaaa ttggataaag tttgtaaaaa
     7021 ggcaggttcg ggcggttcgg cggtttgagg caggggcggc aggcgggata cggcggtctg
     7081 tccggtgttt cagacggcat tgtttgcatt tttcggaaat gaaaaacctc cgggcatccg
     7141 gaggttgcgg tttaaccttg tttttttctt tcggccttgg tctgtttgga caattcggca
     7201 tacaggtcgg gatggtgttt tttcaacagg gcggcgattt ccatatcttt tttgttgact
     7261 tttgccaaat cgaggcattc gatatggacg aggcggagca gttcgcgtac gggtttgggc
     7321 atcttgcggc cggtttcgta gcgtgaacct ccggattgag taacgccgac cttgccccag
     7381 aagtctatct ggttcatccc ggtcttttta cggatggctt taacatcttc tatattttca
     7441 aatattttca taaaacttcc tttaagtttt tctggttgcg gggcaaattc cgatttgctt
     7501 tgttttcgta atgtgccggg tcaagcctgt ttttatattg catacagctt cagggtttgc
     7561 cggtggcggg gctgacggtg aggatttcgt agccggtttc ggtcaccaag acttcgtgtt
     7621 cccattgggc ggagagggag cggtctttgg taaccaccgt ccagccgtcg ttgaggatac
     7681 gcaggtggcg tttgccttgg ttgatcatcg gttcgacggt aaaaatcata cccggtttta
     7741 gaacgggacc ctgccctttt tttccgtagt gcaacacttg cggggcttcg tggaaaccgc
     7801 gcccgatgcc gtgtccgcag aattcttgta caaccgaata acctgcattt tcggcaacct
     7861 gctggcaggc gtaacctacg ccgcccagcg tcgcgccagg tttgacggct tctatgcccg
     7921 ccatcataga ggcgtgggtt acgtcgatca ggcgttgggc gatgggggag actttgccga
     7981 cggtaaacat acggctggag tcgccgtgga aaccgtcttt tttgatggtg aggtcgatgt
     8041 tgataatgtc accttctttg agcggcttgt cgtcgggaat gccgtggcag atgacgtggt
     8101 tgacggaggt gcagcaggat ttggggtagg gcgggttgcc gtagtggagg ggggcgggat
     8161 agccgccttg gacgttgacg tggtagtcgt aaacaagttt gtcgatttcg tcggtggtga
     8221 tgccgggttt gacgaattgt ccgatgtagt cgagggcttc ggcgacgagt ttgcccagct
     8281 cgcgcatttt ttcgatttct tcgggggttt tgatgatgat gccgttcata atgcttcctt
     8341 ggattgcggg tgtgcctgcc gtgccgggtc aaaagaggtt gtcgagaatc tctttatggg
     8401 gtttggccgg cgtgtttttc ggggtgtccg gtttggtatg gttttctttg ggcgtttctt
     8461 tttctttggg tgtttctttg ggctgcgcct gtttttcggg tgtttctttt cggggttcgg
     8521 gatggggggc gtttgtgggc agtacggggg tttctttgag tgtgttttca gcttgtgttc
     8581 cggtgccggt taacgaatcg gctgcccggg ccgcatccga agggcgcggt ttgacccggt
     8641 ttttcttggg tgcggcaggg ggcggcagtc cgctgccgct ttcttctgtg ccgtccggct
     8701 gtgcgggttc ggatgaggcg gcgtggtggt gttcgggttg ggcggcatct tgtccgacgg
     8761 cgccgtttgg cagccagaat tcggtttcgc gggggctgtc ggtgtgttgc ggttcggttt
     8821 ggagggtttt gtcgccgttc gggttgaatg tgctgacgat gcctactgcc aatgcgccga
     8881 tggcggtgat acagatgagc aatacggcgc gtatcaggag tttgggggtc agcctgaagg
     8941 gtttgttcgg tttttttgcc attttgattg tgcttttggg gtgtcgggca atgccgtctg
     9001 aaggcggttc aaacggcatt gccgagtcag cgttggacgt agttttggta gagggtaatg
     9061 actttttgta cgccgacggt ggtgctgact ttttgggtaa tctgcgcctg ttcttcgggg
     9121 gtgaggatgc ccataacgta ggttacattg ccgtaggtaa tgattttgac gcgcgcctgt
     9181 gtagcggggc tgatgcccag cagcgtggcg cggactttgg acgtgttcca agtgtcgccg
     9241 gcgatgtcgc ccgcagtgcg cggcagggag gcgacggtaa tgtagttgta tacgccttcg
     9301 gcggcctgtt cggaacgtgc aatctgaccg acgaactgtt tttcgccttc ggtggcgact
     9361 tgtccgagca gcagcaggtg gcggttgtag ccgacgacgg agatttgggg cgtgtagcct
     9421 ttggtttggt tgttttggcg caggtaggaa cgggcggtgg tttcgatacg caacgccata
     9481 acgttgtcat cggtttgcgc gccggtggtt cggcggtcga tgacggattt cgcgccgacg
     9541 gcggccccgc cgacgactgc gctgaagcag ccgccgaggg caaggctgag gacggcggca
     9601 atcagggtgc ggacggtgtg tggtttgggt ttcatcgggg acttcctttc ttgggcgttt
     9661 cagacggcat tgctttgcgc catgccgtct gaaaagggtt acattccttc cagcagtacg
     9721 gagtcgatac agtcgcacat ggcgtgtatc agcaggatgt ggttttcctg aatgcgggcg
     9781 gtgcgcggat gggggacgtt gagcagaacg tcggtgtctt tgagcatggc ggcgattttg
     9841 ccgccgtcgc ggccggtcag ggcgatgacg tgcatatcgc gttcgtgcgc ggctttgacg
     9901 gcttcgatga cgttggcgga attgccggag gtggaaatgc cgacgaggac gtcgcctgca
     9961 cgtccgagcg cgcgcacttg tttgctgaat acgtggtcga aaccgtagtc gttgccgatg
    10021 gcggtcagcg cggaggtgtc tgtggtcagg gcgacggcgg cgagttccat gcgttctttc
    10081 tcaaaacgcc cggtcatttc ggcggcgaag tgttgcgcgt cggcagccga accgccgttg
    10141 ccgcaggcca ggattttgcc gtcgttcatc aggcattgca gcatcagctc ggcagcctgt
    10201 acggtcggct cgaccagtat tttttcggct tcctgtttgg cacggatgct ttcggcaaaa
    10261 tgtgcggcaa cgcgttcttg taatgtcgtc atatcgtcaa cctgtaatat tctttatcca
    10321 ctcgggcggg cggtttcctt cgataagtac cgcatcgagg cggcacggta cgtttgtcag
    10381 cctgttctgt tgcagataat actctacact tcgttgcagt ttcaataatt tggacggaga
    10441 aatgctgtat gccgcgccgc cgaaccgttg gtttttgcgg tattttactt caacaaacag
    10501 aatcatgccg ccgtttttga caatcaggtc gatttcgccg taggcgcagt gccagttgcg
    10561 ggcaagcagc gtgcagcctt gggattggag gaaggcaagt gcggcatctt cccctgccgt
    10621 accctgtttg tggtttaagc gcataatcgg tttaaccttt ataatgtctg tttttcagac
    10681 ggcatttttt atgtttcaga aacacttgca gaaagccccc gacagcgtcg tcggagggac
    10741 attatacgtg gttgccacgc ccatcggcaa tttggcagac attaccctgc gcgctttggc
    10801 ggtattgcaa aaggcggaca tcatttgtgc cgaagacacg cgcgttactg cgcagctttt
    10861 gagcgcgtac ggcattcagg gcaggttggt cagtgtgcgc gaacacaacg agcggcagat
    10921 ggcggacaag gtaatcggtt tcctttcaga cggcctggtt gtggcgcagg tttccgatgc
    10981 gggtacgccg gccgtgtgcg acccgggcgc gaaactcgcc cgccgcgtgc gcgaagcagg
    11041 gttcaaagtc gttcccgtcg tgggcgcaag cgcggtaatg gcggcgttga gtgtggccgg
    11101 tgtggcggaa tccgattttt atttcaacgg ttttgtaccg ccgaaatcgg gcgaacgtag
    11161 gaaattgttt gccaaatggg tgcgggcggc atttcctgtc gtcatgtttg aaacgccgca
    11221 ccgaatcggg gcaacgcttg ccgatatggc ggaattgttc cccgaacgcc gtctgatgct
    11281 ggcgcgcgaa atcacgaaaa cgtttgaaac gttcttaagc ggcacggttg gggaaattca
    11341 gacggcattg gcggcggacg gcaaccaatc gcgcggcgag atggtgttgg tgctttatcc
    11401 ggcgcaggat gaaaaacacg aaggcttgtc cgagtctgcg caaaatgcga tgaaaatcct
    11461 tgcggccgag ctgccgacca agcaggcggc ggagcttgcc gccaagatta caggtgaggg
    11521 caaaaaggct ttgtacgatt tggcactgtc gtggaaaaac aaatgatgcc ggatggaccg
    11581 gcaccatagt gcgaacggaa tcaacggaca cgccccgtca tgcggtacag cacgtctttg
    11641 ccttgaacac gatggacgac gaccatggcg acgtgtccga cgacggcggc aaacagcagc
    11701 cagcccaaat tgccgtggaa cgtgttgccc aagtttgcca tccactcgat tttttccggc
    11761 gaaccctgca ttacttcaac gccgaacact ttcaacgggc cgcgtccgcc accgtattgg
    11821 cggatcatgc cgataacggg aacggcaagc atgagcagat acagaatgcc gtgtcctgcc
    11881 gctgccgcct tgctgtcgct ttgcggacgc ttggcgcggt tggcaaccgc ccacacgatg
    11941 cgcaatgcaa tcaccgtcag catgaggaaa ccgaaagatt tgtgcaggcc gaacaggctg
    12001 cccgcccatt ccgcctcttc gtaaattgtc cacaggacaa tggtggtcag aatgccgatg
    12061 aagccggcaa cggtcagcca gtgcagcgcg cgtgtcaacg ggtcgtaaac ggcaggttgg
    12121 tttgtgttca taaaaaaatc cttatcatat gtaacggttt gaacggacat tccgtcgggt
    12181 cggaatgtca aaaaggcgcg attgtaccaa agaagttgga catattttgt ttgcaggctg
    12241 aagatttgct taaaaattca ttaagatggg cagaacaaat agcttctccg cggtatattg
    12301 aaataccgct tataccttat aatgtcggca gaaaatctgt tcagacggca tcaaaacata
    12361 tggcaatccg cctgaaaccc tatttaaaac caacaaacaa gagcatttaa tgaaattcat
    12421 cgacgaagca aaaatcgaag tcgccgcagg caaaggcggt aatggcgcaa ccagtttccg
    12481 ccgcgaaaaa ttcgtacccc gcggcggccc ggacggtggc gacggcggca aaggcggcag
    12541 cgtctgggca gaagccgacg aaaacaccaa caccctcgtc gaataccgct tcgtcaaacg
    12601 ctaccaagcc aaaaacggcg aaaaaggcca cggttccgac cgctacggcg caggtgcgga
    12661 cgacattgtc ctcaaaatgc ccgtcggcac ccttatccgc gacctcgaca ccgacgaaat
    12721 cgttgccgac ctcacctacc acggacagcg cgtctgcctc gccaaaggcg gcaaaggcgg
    12781 cttgggcaac atccacttca aatcctccgt caaccgcgcc ccaaaacaat ccacgcccgg
    12841 cgaagaaggc gaaacccgtt ccctgcaact cgaactcaaa gtcctcgccg atgtcggctt
    12901 gttaggcatg cccaacgccg gcaaatccac cctgattacc gccgtctccg ccgcacgccc
    12961 caaaatcgcc aactacccct tcaccaccct gcatccgaac ttgggcgtgg tacgcatcga
    13021 cgaaaaccac agcttcgtga tggccgacat ccccggcctg attgaaggcg cggcagaagg
    13081 cgcaggcctc ggccaccgct tcctcaaaca cctatcacgc accggtctgc tgttacacgt
    13141 tgtcgatttg gcacccttcg acgaaaccgt caatcccgcc gaagaagcac tcgccatcat
    13201 caacgaatta cgcaaatacg acgaagaact ttacggcaaa ccgcgctggc tggtgctgaa
    13261 caaactcgat atgctcgacg aagaagaagc ccgggcgcga acagccgcct tcctcgaagc
    13321 cgtcggctgg gactaccccg aacctgatga ccgtttccaa ttcgacatgg aaaccccgcg
    13381 cctcttccaa atcagcgcgc tgacacatca aggcacgcag gagttggtac accaaatcaa
    13441 ccaatacctg gccgagaaaa aacgtatcga ggctgaaaaa gccgaagcgg agaaagcagc
    13501 ggcaaatgtt gaaattatcg aacagcagcc taaaacggat actggcgtgt ttaagccgga
    13561 gtaaaaggac aaggccgtct gaaagttttc aggcggctca ttctgcagag atgtgctatg
    13621 tacaaaacaa tagatttatt ttcaggcatt ggcggaatac gcctaggctt tgaaaaatac
    13681 gggtgcacca atgtattctc atcggaatgg gacaaatatg cccgacaagt gtatgaagca
    13741 aactttggag aaaaaccttt tggcgacatt aacggcatcg atccttcaga tattcccgat
    13801 catgatattc tgcttgcggg atttccgtgc caacctttca gtatcgcagg taagggattg
    13861 ggatttgaag atacgcgcgg aacattgttt ttcaatattg ccgaaatcct gaaaaccaaa
    13921 cagccgaagg cttttcttct ggaaaacgtt aagcggctga ctacgcacga ttcgggcagg
    13981 actttccgta ttgttttaga aacccttaaa cagcttggtt atacggttta ttttaaggtt
    14041 ttaaatactt tggatttcgg attgccgcaa aaacgcgaga gaatttatat tgtgggtttt
    14101 tcagacaaca tcccttttta ctttcccgaa cccataaacc aatatcggcc attgggcgaa
    14161 ctgctggaaa acgatcgaga tgtcgaacca agctattttt tatcagatac cttgaaacaa
    14221 aaacgtttgg cagccttaaa aaaagctccg cccaccccat caatttggca tgaaaatatt
    14281 ggcgggaatg tttccgcact tccatattcc tgtgccttaa gggccggggg aagttacaat
    14341 tatttggtgg taaacggagt aagaaggcta acaggaaggg aaatgttaag attacaaggt
    14401 tttcccgatg attttgaaat caatatcccc tactcgcaag tcaggaaagt cgccggaaat
    14461 tctgtatccg tccctgtaat agaagccatc gcggaaaata tgctcgcttc tctttccggc
    14521 aaggtcgaac aaaaagggca attggattta ttggaggcag gatgacgctt gaagaacaac
    14581 aagccaaaga agcattggac ggcattatca aaaaatcccg tgtccacctt tataaaccca
    14641 ttcaaattgc agaaatttta tatcatgacc gttgtataaa acagttggat tttttaaatt
    14701 tagatactta ccgcaaccaa tctaaacgct ggagggatga aatctgccgt cggtttttag
    14761 ggcggatttc tacttcatct gcaaaattcc aagataatct gtttgaaaaa aatgcaatac
    14821 cgcctgaaaa actagcggta ttgggaacat taaacagaca gtcggacggc ggggtagaat
    14881 cgtacattta taaacagttt ttcaatcgtt tttcccaaat gagcgaagca ttggcttatg
    14941 tcggcaatac agacagatac tccttccaac tgtctgaatt tctaaattta ttttggctcg
    15001 agccgggatt gaaaagaagt atagacaaga tatatgaaat cgttgtttat gcgttgtttg
    15061 atgcattggt ttcagaatta ggcataacgg tttcaattga ttttcctaag gaaaacctgt
    15121 ttttatggga ggaatatcaa gattttgccg aaaaaatcat cactatgccg aaaaatgagc
    15181 atttaaaact tcctgcaaaa atccatcgtg taggcgtaac caatgctgcc gatagagggt
    15241 tggatatgtg gtctaacttc ggattggcca tacaggtcaa acatctctct ttagacgaag
    15301 agttggcaga agatattgta tcctccatta gcgcagatag aattgtcatt gtctgcaaaa
    15361 aggcagaaca atcggtgatt gtttcattac tgacgcaaat aggctggaaa agccgaattc
    15421 aaaacatcgt aactgaggat gatttgataa gttggtacga aaaagcattg agaggccaat
    15481 atccgattgc agaagcgttg ttggaaaata ttaaaactga aattatgcgt gaatttcctg
    15541 ccgttaatga agccaatgaa tttttagatt tcgcccaaaa tcgcggatat gacattactg
    15601 ttactcattt ctaaacttac ttgatgaata tgaccgccat ctacaacacc cttacccgtc
    15661 aaaaagaacc cttcgccccc atcgaccctg aaaacgtgcg tatgtacgtc tgcggcatga
    15721 ccgtttacga ctattgccac ttaggccatg cccgtgtgat ggtggtgttc gacatgattg
    15781 cccgctggtt gcgcgagtgc ggttatccgc tcacttatgt gcgcaacatc accgacatcg
    15841 acgacaaaat catcgcccgt gcggctgaaa acggcgaaac catcggcgaa ctgaacgcgc
    15901 gtttcattca ggcgatgcac gaagatgccg atgctttggg cgtgttgcgt cccgacatcg
    15961 agccgaaggc gacggaaaac atcccgcaaa tgattgccat gattgagacc ctgattcaaa
    16021 acggcaaggc atatcctgcc gcaaacggcg acgtttacta cgccgtgcgc gagtttgccg
    16081 cttacggaca attatcgggc aaatcgttgg acgacctgcg tgcgggcgaa cgcgtggaag
    16141 tggacggttt caaacgcgat ccgcttgatt ttgtattgtg gaaagcggcg aaagcaggcg
    16201 aaccggcgtg ggaaagcccg tggggcaacg gccgtccggg ttggcacatc gaatgttccg
    16261 cgatgagtga aaacctgttc ggcgatacgt tcgacattca cggcggcggc gcggatttgc
    16321 agttcccgca ccatgaaaat gaaatcgccc aaagcgtcgg tgccagcggt cacacctgcg
    16381 gtcatgacca cgcgcaaacc caccacggtc aaagtattgc cagccacgtc aaatactggc
    16441 tgcacaacgg cttcatccgt gtggacggcg aaaaaatgtc caaatcgctg ggcaacttct
    16501 ttactatccg cgaagtattg aaacaatacg accccgaagt cgtgcgtttc ttcatcctgc
    16561 gcgcccacta ccgcagcccg ttgaactact ccgacgcgca tttggacgac gcgaaaggcg
    16621 cgttgacccg tctgtacacc accttgaaaa acacaccggc ggctgagttt gatttgtctg
    16681 aaaacgccaa cggctacacc cgccgtttct acgccgccat gaacgacgat ttcggtacgg
    16741 tcgaagcggt tgccgtgttg tttgagctgg caggcgaagt gaacaaaacc aatgacgcac
    16801 acctcgccgg ctgcctgaaa gctttgggcg gcatcatcgg cctgctgcaa cgcaacccga
    16861 ttgagttcct ccaaggcggc gcagtttcag acggcctctc caacgaagaa atcgaagact
    16921 tgattgcccg acgcaaacag gctcgcgcgg ataaaaactg ggcggagtcc gaccgcatcc
    16981 gcgaccttct gaacgaacac aaaatcattt tggaagacag cgccggcggc acgacttggc
    17041 ggcgcggttg aactgtatcg gaaagggcaa aagtaccgcc ttttgccctt ttttcgtttt
    17101 cagacggcat ttcccgcttg aagccccgcc gttttcagca ggcaggaccg aatgggaaat
    17161 accgggcggc gcaccgggtg ccgctttgtg tatgggaaca ttgtgagaac ttatttttat
    17221 tatgcttaaa atcatttccg ccaacgtcaa cggcatccgc tccgcctaca aaaaaggttt
    17281 ttacgaatac atcgccgcat cgggcgcaga cattgtctgc gtgcaggaac tcaaagcgca
    17341 ggaggctgat ttgtctgccg atatgaaaaa tccgcacggg atgcacggct attggcattg
    17401 cgccgagaag cgcggttaca gcggtgtggc ggtgtacagc aaacgcaaac ccgacaatgt
    17461 gcaaatcggc atgggcattg aggaattcga ccgcgaaggg cggtttgtgc gttgcgattt
    17521 cggcaggttg ggcgtcattt cgctttattt gcccagcggc agcagcgcgg aagaacgcca
    17581 gcaggtgaaa taccgtttcc tcgatgcgtt ttaccctatg ctcgaagcga tgaaaaacga
    17641 agggcgcgac atcgtcgtct gcggcgactg gaacatcgcc caccaaaaca tcgacctgaa
    17701 aaattggaaa ggcaatcaga aaaattcggg cttcctgccc gaagagcgcg agtggatagg
    17761 caaagtcatc catacgctgg gctggacgga catgtggcgc acgctctatc ccgatgtgcc
    17821 gggttataca tggtggagca accgcgggca ggcgtatgcg aaagatgtcg ggtggcgcat
    17881 cgattatcag atggttacgc ccgaacttgc cgccaaagcc gtgtccgcac acgtttataa
    17941 agatgaaaaa ttctcggatc acgccccttt agttgtggag tatgactatg ctgccgaata
    18001 aggttttggg taaatatgat tggaatgtgg acggaaaaac aggaataggc gcggcatggg
    18061 ttgtcgctgc gtttatcctg cccatattgg tgtgggcggt gtttatgttg tcgcggatgc
    18121 aaggctggct tgcgccgacc aaggcaaacc cgatatgggc gttggtgtgg ctgctgattt
    18181 gtctgccctg cctgctgatt gcggccaaat gtttgggttg gaaaggctgg cggcgggtgg
    18241 tgaatatttt tgtctgcctg accgtctgcg ccatattgag cgtacccgct tcgctgctca
    18301 tcgcctttac cctccgggac ctgctcaaat agtcgcggac aatggaaagt ttcgagcttg
    18361 aggatttgac gctgtggctg atacgcgatg cggatgaagc ggaaatgtgg atagaccgtt
    18421 gggcggtcag ttatcccgtt gtgcagatgt ccgaagcgtc cgcaaacggc agcacggaag
    18481 cgtggcaaag cgcgcttcag acggcattcg aacggataca gggcaggcat atcgccgttg
    18541 tcgcacacgg cgcgggcgcg gccgcattct tggcgtggct gtaccgcgcc gacatcctga
    18601 cgcagaagaa actcgccggt atcattctcg tatctccgcg tcccgatatt tttcccgacg
    18661 atgcggaaca cactttccaa cgcgtccgct gtccttgccg tgccgcattg gtggtgtccg
    18721 aacacggcgg cgtgccgcac ggttgggcgc aaaaacaggc ggatttgtgg aacgcccgcc
    18781 tgttggtttc cccgcattcg ggcagtttga acggtatgct cggcggctgg cagtggggta
    18841 tgaagctgat gcaggaaatg ttgctggcgt gaacggtcgc tttatcggaa tttcggttaa
    18901 ccggggttgc aaggaaaata ccgtctgaaa caggcttcag acggcatttc cattcaaaca
    18961 gggcttacag gctgcccaac acgatacgca acacgcggcg catcggttcg gctgcacccc
    19021 acaaaagttg gtcgccgacg gtgaacgcgc tgatgtattc gccgcccatg cccagtttgc
    19081 ggatgcgtcc gacagggacg gacagcgtgc cggtaacttt ggcgggcgtc agctcgcgga
    19141 tgctggcttc tttttcgttg gggacgactt tcacccagtc gttcgcgcct gccaaaatcg
    19201 cttcgatttc ggaaacaggc aggtcttttt tcaacttcag agtgatggct tggctgtggc
    19261 agcgcatcgc gccgatgcgg acgcacaggc cgtcgatgac ggtcggattg ccgctgcgtc
    19321 cgaggatttt gttggtttcc acgccgccct tccattcttc tttggactgg ccgttgccca
    19381 aatccacgtc aatccacgga atcaggctgc cggcgagcgg tacgccgaag ttggctttcg
    19441 gatagtcttc gctgcggagg aaatcggaca ctttgcggtc gatatcgaga atcgcgctgg
    19501 aaggatcggc aagctcgtcc gccacttggg cgtgaatcgc gcccatgccg ctgatgagtt
    19561 cgcgcatatt tttcgcgccc gcgccggaag cggcttggta ggtcatgctg gtcgcccatt
    19621 cgaccaaatc gttttggaac aggccgccca aagccatcag catcagggag acggtacagt
    19681 tgccgccgat gtagtttttc acgccgtttt tgagaccgtt gtcgatgacg ttgcggttga
    19741 cggggtcgag ggcgataatc gcgtcgtctt tcatgcgcag tgaggacgcc gcgtcaatcc
    19801 agtagccgtt ccagccgctg tcgcgcaggg cttggaagac ggatttggtg taatcgccgc
    19861 cttggcaggt gacgataatg tccattttcg ccagctcggc aacgtcgttc gcgtccaata
    19921 atgttttagc cgcctgaccg aaatcaggtg ccgcgccgcc gacgttggaa gaggtaaaga
    19981 aaaacgcttc gggaatgtgg gcgaagtcgt tttcttcttt catacgctgc atcaaaaccg
    20041 aaccgaccat accgcgccag ccgacgaaac ctactttcat gtattgctcc ttgaggaaaa
    20101 tcgttaataa aattatgtat ggcgtaaaga aagcgtttta acgccgtatt gggaaaatgt
    20161 aaagttcttt atcgaatttt ctttttggga ttggcgtttg cagcagtagg gcggtagaat
    20221 tccatctgtt taagaacaat tatcaggaac ggatatggaa gaaaaaaacg actacaccga
    20281 cgcggtatcc gacaaccgca acgggcagga aattgaagtc ggaattgccg gcgcaggcga
    20341 ccggatactt gccgcgctgc tgaaccaatt gtttaccttt ttgattttgt tggtgccgtt
    20401 tgtcggactg attgccttcg ccgtcaaaaa tgaaggcagg attggcggtc gtgaggaaat
    20461 attcgggctg cttttgggca tgacttcctt ttgggtcgga ctggcgggca ttttggcgta
    20521 caccgtcatc caaatctact atatgagcag ggacgggcag tcattgggta agaaaatcat
    20581 gagaatccgc gtgttgaaaa ccgacggccg caatccgggt tttgtcggca cggttttggt
    20641 acgcgaaatc gcgtggtcgg ttttggttgc cattattgcc gccgttatcg gtcttgcagt
    20701 aggtgaaaac ggggaaaacg ccatcaacct gctggcattc cttgccaact ttgtcctgct
    20761 ctttatggtc aaacgcgacc gccgcacgct ttacgacata ttggcggata cggttgtcgt
    20821 caagctgccc aaataaacgg acgcggcaaa atgccgtctg aaaggctttc agacggcatt
    20881 tttatcttca gaaccgctca agcatcgtgc agcgaagctg cgtgcaaagt gttttccatt
    20941 aatgtggcaa tcgtcatcgg acccacgccg ccgggaacgg gcgtaatcat cgccgcccgt
    21001 tcttttgccg tttcaaattc cacgtcgccg cacaggctgc cgtcgtccaa acggttgatg
    21061 cccacatcga taacgaccgc gccgggtttg atccacccgc ctttgacaaa gttcggaatg
    21121 cccacgccga ccaccaaaat atcggcggcg gcaacttcat cggcaagatt ttcggtcgca
    21181 ctgtggcaga ccgttaccgt tgcgcgcgcc agcagcaatt ccaacgcctg cgggcggccg
    21241 acaatattcg acgcgccgac cacgaccgct tttttcccct tcggatcaat gccgtaagcc
    21301 tccaaaagcg tcatcacgcc cttgggcgta cacgggcgca tcagcggcat tttgaccgcc
    21361 agcctgccga cattgtaagg atggaagccg tccacgtcct tatccggcga aatacgttcc
    21421 aagatcgctt ggctgtcgag gtgcttcggc agtggaagct gaaccagaat accgtccact
    21481 tcggaatcgg cattcagacg gtcgaccaat gccagcagtt cttcctgcga tgttgattcg
    21541 ggcagttcgt acgacagcga cttgatgccg catttttggc aggcggtttt cttgttgcgg
    21601 acataaactg cgccggcagg gtcgcctccg accaaaacca cggccaggca aggcgtgtgc
    21661 agattgtcct gttggcgttg cgccaccgct tcggcaatcg cctgcaggtg tttttgcgaa
    21721 acttctttac cgttgatcag ttgtgccgac atatccgtcc ttttgttaaa caggtagaaa
    21781 atgctgaaaa ttatcgcatt ttccgtttgt ttttacagca gaatgccgaa aataaatttg
    21841 ccccgaaata aaatacggct gtttggacgg attaaatatt ttttcaaatc aaaaaactat
    21901 atttttcatc ttggtgtttt gaaaataaat gttgacgcat ctttccctga actgtatatt
    21961 ccgcttcttc aagtcggggc gtagcgcagc ccggttagcg catctgcttt gggagcagag
    22021 ggtcgtgagt tcgaatccca ccgccccgac caatcaatca gcacgaatcg gcatgagcgc
    22081 ccgtagctca accggataga gcaccgacct tctaagtcgg gggttacagg ttcgattcct
    22141 gtcgggcgtg ccaagaattt gattgaaaac cgtttttatg gtggctgtag ctcagttggt
    22201 agagccccgg attgtgattc cggttgtcgt gggttcgagc cccatcagcc accccagata
    22261 aaggcccgta cttttcggta cgggtctttt cctgtttgcg cgtactgtcc gcgccgtcgt
    22321 accggtgcgg cggtatgccg tctgaaagcc gggccggtca tacggaaacc gcttcatacg
    22381 gcatttgcgc cgccggcttt gcctttcgca tatgcttctg tatcatgtgg gtttttctgt
    22441 cttttatggt ttttgtcgga ggctttgatg gttgacgctt ggtctgtccc tgattttgaa
    22501 tcgaaaatct gtccgcccga ggcgttggcg gcgcgtttgg cgttgttgcc gcgcccgctg
    22561 gtgtttacca acggctgttt cgacatcctc cacagggggc acgttactta tctggcgcag
    22621 gcgcgttcgg caggggcggc gttggtgctg gcgttgaata ccgatgcttc ggtgcggcgt
    22681 ttgggcaagg gcggcgaccg cccggttaat tctttggaga accgtgccgc cgttgccgcc
    22741 gcgttggaaa gtgtggattt ggttacttgg tttgacgagg atacgccggc ggcgttgatt
    22801 gaggcggtca agccggaggt gttggtcaag ggcggcgatt gggtcgtgga taagattgtc
    22861 ggtgcggcgg aaacgctggc acgcggcggt caggtgtttt caattccgtt tctgcatcag
    22921 acttcgacaa cgaagacttt ggcaaaaatc cgtgcggcag agggcggaaa atgacggttt
    22981 tgaagccttc gcactggcgg gtgttggcgg agcttgccga cggtttgccg caacacgtat
    23041 cgcaattggc gcgtgaggcg gacatgaagc cgcagcagct caacggtttt tggcagcaga
    23101 tgccggcgca tatacgcggg ctgttgcgcc aacacgacgg ctattggcgg ctggtgcgcc
    23161 ccttggcggt tttcgatgcc gaaggtttgc gcgatctggg ggaaaggtcg ggttttcaga
    23221 cggcattgaa gcacgagtgc gcgtccagca acgacgagat actggaattg gcgcggattg
    23281 cgccggacaa ggcgcacaaa accatatgcg tgacccacct gcaaagtaag ggcagggggc
    23341 ggcaggggcg gaagtggtcg caccgtttgg gcgagtgcct gatgttcagt ttcggctggg
    23401 cgtttgaccg gccgcagtat gagttgggtt cgctgtcgcc tgttgcggca cttgcgtgcc
    23461 ggcgcgcttt ggggtgtttg ggtttggaaa cgcaaatcaa gtggccaaac gatttggtcg
    23521 tcggacgcga caaattgggc ggcattctga ttgaaacagt cagggcgggc ggtaaaacgg
    23581 ttgccgtggt cggtatcggc atcaatttcg tgctgcccaa ggaagtggaa aacgccgctt
    23641 ccgtgcagtc gctgtttcag acggcatcgc ggcggggcaa tgccgatgcc gccgtattgc
    23701 tggaaacatt gcttgcggaa ctgggcgcgg tgttggaaca atatgcggaa gaagggttcg
    23761 cgccattttt aaatgagtat gaaacggcca accgcgacca cggcaaggcg gtattgctgt
    23821 tgcgcgacgg cgaaaccgtg tgcgaaggca cggttaaagg cgtggacgga cgaggcgttc
    23881 tgcacttgga aacggcagag ggcgaacaga cggtcgtcag cggcgaaatc agcctgcggc
    23941 ccgacgacag accggtttcc gtgccgaagc ggcgggattc ggaacgtttt ttgctgttgg
    24001 acggcgggaa cagccggctc aagtgggcgt gggtggaaaa cggcacgttc gcaaccgtcg
    24061 gcagcgcgcc gtaccgcgat ttgtcgcctt tgggcgcgga gtgggcggaa aaggcggatg
    24121 gaaatgtccg catcgtcggt tgcgccgtgt gcggagaatc caaaaaggca caagtgaagg
    24181 aacagctcgc ccgaaaaatc gagtggctgc cgtcttccgc acaggctttg ggcatacgca
    24241 accactaccg ccaccccgaa gaacacggtt ccgaccgttg gttcaacgcc ttgggcagcc
    24301 gccgcttcag ccgcaacgcc tgcgtcgtcg tcagttgcgg cacggcggta acggttgacg
    24361 cgctcaccga tgacggacat tatctcggcg gaaccatcat gcccggcttc cacctgatga
    24421 aagaatcgct cgccgtccga accgccaacc tcaaccgccc cgccggcaaa cgttaccctt
    24481 tcccgaccac aacgggcaac gccgtcgcaa gcggcatgat ggacgcggtt tgcggctcga
    24541 taatgatgat gcacggccgt ttgaaagaaa aaaacggcgc gggcaagcct gtcgatgtca
    24601 tcattaccgg cggcggcgcg gcgaaagtcg ccgaagccct gccgcctgca tttttggcgg
    24661 aaaataccgt gcgcgtggcg gacaacctcg tcatccacgg gctgctgaac ctgattgccg
    24721 ccgaaggcgg ggaatcggaa cacgcttaaa aaatatgcct tttttcaaca cggggcggca
    24781 tccgtccgaa tctgtccgga ttctgccgcc cgacagactg ccgcccgggc gggcggggtt
    24841 tcaaacattg aggaaattct aatgaaatgg ctcttcaccc ttttggcggt actcaatatt
    24901 gccgtattcg gcggcacggt gggctataaa ttggccgtta aagcggcggg cggcgtgccg
    24961 gaaaaccggg ccgtcgaaaa cacgcccccc gcaacgcccg ccgccggcaa tgcggcggca
    25021 tccgtggagg atacggcggc actgctcaaa ccgggcgaca tcctgagcga agaacaggcg
    25081 gagcagttgc gcttgaaaaa agaagcggaa cagaaaaaac tgagggagaa aaaacagcgt
    25141 gaagaaaaag cccgccgcga aaaactcgcc gccgaaaagg cgcaggcgga acgtgaaaac
    25201 ggcgcggcgg atgccttatg cgccgcgcag gcaagcctca cgatggacga agacgactac
    25261 caccgcatca aagggctttt gggcaaatgg tcgcacgttg ccagcaggag cgtcgaaaaa
    25321 cgcaccgcca aagccaaacc tgccgacaaa acctaccgcg tcgtcctgcc cgtttccgcc
    25381 gatgccgaaa atcaggcggc ggagctgtct gccaaaggtt tcaaccccgt accgtttgac
    25441 ggcgcattga gtttgggtgt cggcaacagc cgggaaaacg cccaagccct gcaaaaccga
    25501 cttgccgatg ccggattcgg cggggcgcat attgtcgaac actttgccga agccgacagg
    25561 caggacgatt ctttgagcgt gtcgcgtatg acggttttgt ttacgggcgt gaatgccgcc
    25621 gatgcggacg aaatccgtaa aatcacgtcc ctatacggca aactgaacct caagtcttgc
    25681 aaataagtgc agaccaaaaa cgccaaaccc accgctaggg tgggtttggc gtttcggttt
    25741 tgagtatctg attaaaaagg gaatgtatgt ctcgtatatg ccgactccaa gtgtgaaagt
    25801 gaattgtgag ggaatacagt gtttaatggt tcggaacgga cggcttccga gtggttttga
    25861 ttatacgcta ataataataa ttatcaataa aaatttaaaa ataaatgcgg attttttgta
    25921 atgcgatgaa aaataaacaa attaatctgt ccgccgatgt ttgtgatttg ggtcggcaaa
    25981 ctttcattgc gccggacaaa tcggggtcgg caggcatgga cgggggcttc gtgcctgtcg
    26041 gaaagaagcc tgccgtgttt gaacaaccgt ccgatttggt gcggacaggg ttgccattcg
    26101 ggggtgtctg agcgcggatt cggggatgca aaagcggtga aaccgttgcg gatgaaggct
    26161 tacggatgcc ccgaggtatt tttaaatgta ttcaaacgac aaaactccct tctattgccg
    26221 tacttagggc ttgcgcagca gcctgcgcgc cggggagtcc gggtgtttcg ttggttgcgg
    26281 tgtcggggcg ggcggcaagg ttttccggcc ggtttccctg ttcctcacga caaagccgtg
    26341 atgccgggga atggcggttt gcccgtttgg ggccgcgatg ccgtctgaag ccttcagacg
    26401 gcatttttgc cgcctttttc aatattccgc cgaatgccaa aacggttgcc cgactgtcgg
    26461 cgtgctggct tgggctttgg ttcgcgcttc gaccggcccg gcttcaaatg ccagccgtcc
    26521 ggattcgacg gcgagtgcga aggcgcgcgc catgttgacg gggtcgccgc tgcgggaaac
    26581 ggcggtgttt aacaatacgc cgtcaaaacc ccattccatc acttgtgccg cttgggaagg
    26641 caaacccaag cccgcgtcga taatcagcgg cgtgtcgggc aggcgttcgc gcaggatttt
    26701 gagcgcatag gcgtgaaccg cccccaaacc cgtgccgatg ggagccgccc acggcatcaa
    26761 cgcctgacag cccgcatcga gcaggcggcg gcaggcaatc aggtcttcgg tgcaataagg
    26821 caacactttg aagccgtctt taatcaggat ttccgccgct tcgacgagtt ggaacacgtc
    26881 cggctgcaag gtgtcgtcgt cgccgatgag ttccaatttt atccaatcgg tttcaaacac
    26941 ttcgcgcgcc atttgcgccg tcgttaccgc ttcctgcacg ctttggcagc ctgccgtgtt
    27001 cggcaggacg ggaacgccgg tttcttgaag cagcgaccaa aacccctgac cgtgcgcctc
    27061 gccgccgcat cccgtgcggc gcagcgagac ggtaatcatc gcgggccggg cggttcggac
    27121 ggattgtttg aggatttcag gggtcgggta ggcggctgtg ccgagcagca gccgcgaagg
    27181 gaaagtttcg ccgtacaggg tgagcatgat ggattctttg gtatggttgt cggacgcgat
    27241 gccgtctgaa agccggcggc gtgcctttgg ggtttcagac ggcattcagc cgccgacgac
    27301 ggggcggacg atgtcgattt tgtcgttttc gtgtaaaacg gtttccgtat acgcgccttt
    27361 ggggatgaaa acggtgttga ccgccacggc aaagggtttt tgcggcgcgg tttgggcgat
    27421 gaggtcggca acgcttgtgc cgtgaagttc ggcgggtccg ccgtttaaga tgatgttcat
    27481 ggtttctcga tgtctctgta tttatagcgg attaacaaaa accggtacgg cgttgccccg
    27541 ccccggctca aagggaacga ttccctaagg tgcccaagca ccgggcgaac cggttccgta
    27601 ctatttgtac tgtctgcggc ttcgccgcct tgtcctgatt tttgttaatc cactatatct
    27661 agccgaatta ctttattttt tgatacgcaa ccggccggtt gccgtcattc ccgcgcaggc
    27721 gggaatccat ttttgaaatt cggaaactgt ttttcaaatg atggtttctc aagttttacg
    27781 gcggattccc gcctgagcgg gaatgacgga atttgatgat atgccacatt taaagttaag
    27841 catatttgcg ataacgggct aagttttgcc tacacggcaa ttgaattttc ttctttcggt
    27901 tttatccatc ccacaaagcc tgaaacgctt taaccaccgc ttcgggattt gccgcttcgg
    27961 ttacggcgcg gacggcggcg agtgaggaaa ctccggtggc cagcacggct cgggcgttgt
    28021 tcaaatcgat gccgccgatg gcgacgacgg gcgtgccgcg agcctgttcc acatattcgc
    28081 gcaatttgtc caagccttgc ggggcggtgg gcatttgttt ggtcgtggtc tggaaaatcg
    28141 cgccgctggc gatgtagccg gggtgtacgg acagggcgcg gtcgagttcg gcgacggagt
    28201 gcgtactcaa acccaaacgc aaaccggcgg cggcgatggc ggcaaggtcg gcggtgtcca
    28261 tgtcttcctg cccgagatgt acgccgtatg cgcccgcttc gattgcctcg cgccagtggt
    28321 cgttgatgaa aagctgtgtg cggctgcctt gacaggcggc gacgcagcgg gcgatttcgc
    28381 gtttcaattc gttgccgtgc aaggttttac agcgcagttg caccgtgtcg gcacctgctt
    28441 tgaccatgcg ccccacccaa tcggcggtgg gaacgacggc gtagaattta agtagggatt
    28501 ttaggggcgg gaaggtcata gggtgtcctt tcgggcggct ttaatcttgt cttccgatat
    28561 acgccaaacc gctttcttca tcacgttcgg gcgcgtcttt tccgtcaaac agtgccactg
    28621 ccaatctgac ggcggcggcg gttacggcgg gggaaatcat aaagccgtgc cggaaaaggc
    28681 cgttgatttc gatgaggcgg cgttcgcggc tgtagcggat ttcggggttg tggtggttga
    28741 gcgtggggcg caggccggcg gcgatttcga ggatgtcggc ttcgccgaag gcggggtgga
    28801 cggcatagag cgcggataag agttccagcc cggaacgtac gctggcgggg gcttggcttt
    28861 cgctttcgat ttgggtcgcg ccgatgacga agacgtggtt ttctttcggg gcgatgtaga
    28921 gcggatagcg cgggtgcagc aggcgcacgg ggcggttgag cgtgatttcg ggcgtgtaaa
    28981 cccgcgccac ttcgccgcgt atgccgcgca aggtgctggt gtgctcgggg gattggttcc
    29041 acgcggtttt cgcgccgtag ccccggcagt cgattaccca gtcgtattgg gcttgcaggt
    29101 cttggggggc gcattcgtgt tcccaatggc aagggacgtt cagttcgtcc aaagcgtcgg
    29161 caagtgcaga caatatttgc cgcccgtcga gctggccttc ggtcggcagg tagatgccgt
    29221 ctgaaaaacg tccgccgagt tgcggttcgc gttcggcgat ttcatcggcg cgccaacgga
    29281 cgatttcgtc atccgctacg ccgccgcgtt tgagatggcg gacgaactcg ctggataatg
    29341 gcttgtcctg cccgtgccac acaatcaggc tgccgttttc ctgcatcatc gtgtgcgtgt
    29401 tcagacggca tcggatgccg cgccaaagcg gaatgctctg cctgcccagc ctgatgactt
    29461 cgggcgttgc ctcgaccgct tccgccgcag gcgcgagcat cgcggcggca acataggcgg
    29521 cggcgtgttc gccttggcgg gtgcccttgt cgaaaagttc aatctgataa ccttgttctg
    29581 caagctgcaa tgcggtcagc cttccggaaa ggccgcctcc gaggacggcg atacgggtca
    29641 tgacgggttc ctttgtaaag attgggtttt tttaaagaaa aggcgtaccg atacggtggc
    29701 aatggcaacg gcagacatta cgggggcggt cagaccgatg ctgctttccc aaccggacga
    29761 gagcaggaag cggtagagga tgaagcctgc cagccacaga accagtccgg caaagtcaaa
    29821 gccttcaatc tcctcacgcc gttttaagac gaaaaagtcg gcaatcaaaa ccgccgccat
    29881 cggcgcaaat accgagccga taagcagcag gaagttttca tattcggtaa cgggcagcat
    29941 gacggcaagc accgtgccga tcagggtaac gccgacagcg acgggtgttt ccgcaaaacg
    30001 cgcggaaatg ttgttcgcac tcgcgccggc ggaataggta tcgagaaacg ttgtggtaac
    30061 ggtggagagg acgactgcca gaatgcccgt tatgcccaag cccgcgccca acaggatttt
    30121 cgccacgtcg gtttctccgg taaacagagc cgccgccaaa cccaaggcat acatccagca
    30181 gcccgtcagc gtataggcga gcgttgccgt cagggttgcc gcaaacgggc ggcgtgcttg
    30241 gcgcgtgtag tcggcggcca gcggcagcca ggaaagcggc atgacggcgg acagtttgac
    30301 tgccgttccg aaggtcatgc cgtctgaaac ggcgggcgcg gcgtttgtgc cggacgaagc
    30361 gaacacttcg acgctcaacc acaacacggc aagcagcatc agcagcatcg aaacggtttt
    30421 cagcccgccc gttctgcgtg cgccgaaaac cagccacagc acgatcagtg cgccgtttgc
    30481 caatgcccac cagacaaagg attcgccgtc ccacaacact ttgcccaaag cggagctgac
    30541 cgttgcgccg acgtaaatca tcaccgccgt ccagccggcc agttgcagca tattcgccac
    30601 ggaaaacagc actgaaccgc atttgccgaa cgacaggcgc acactttcca tcgagctgcg
    30661 tccggtcagt gcgccgatat acgctgccgc aaaaaacagc gcgccgccga cggcatgacc
    30721 caaaagcagg gccgccagac cgcgctgcca gcccaagggg gcgagcagcg tacccgtgct
    30781 gatttcggca atcgataccg ccgcgccgaa ccaaatcagc ccgatggcgg cggaagatga
    30841 aggagaggag gcattgcccg acatatgacg tcctttaaaa agaaggaagg tgggcgggga
    30901 agtgcggaaa agggaacgga agcccgaaag ggcggggagg ttttgtttcc tacgcaggca
    30961 ttaccccgac aggttctacg gattttgctt tcgcaatctc agccgcccgg cgggcggcac
    31021 tccgacaaga aggctttaag tttacacaaa gcaacgtgcg ttggcaaaat tcttacattt
    31081 tcgacggcac ggcgcatttc cataacaaat gccgtataat caagcccctg attttttatg
    31141 cgccgacact atgcatccga cctattccgc cgtacaggcg cggctgctcg aagccaaccg
    31201 cctttccccc gaactgctcg ccaaaagcct gtgcatcatc ggtgcgcacc acgtcgatta
    31261 tgccgatatc tactgccagc gcaccgctta tgaaagctgg catttggaag agggcatcgt
    31321 caaatcgggc agcttccaaa tcgatcaggg tgtgggcgtg cgcgccgttt cgggcgacaa
    31381 aaccgccttt gcctacgccg acagcctgtg catcgattcg ataaaccgtt ccgcccgcgc
    31441 cgtccgcgcg attggggcgg caggcggcaa ggtgtccgcc aaaatgccgt ctgaaacgcg
    31501 cggcaagccg gtttgttccg catccgaccc cattgccggc ctcgattccg ccgccaaagt
    31561 cgcgctgttg aacaaagtgg aagcaatcgc caaagccgcc gatccgcgca tcgtgcaagt
    31621 gatggccggt ttgacctgcg aatacgatat ggtttacctc gcccgtctgg acggcaaaca
    31681 cgccgccgac atccgcccga tggtgcgcct gaacgttacc gtcatcgcca aacagggcga
    31741 acgccgcgaa cagggcggcg cgggcggcgg aggacgctac gacttggctt atttcgatga
    31801 aaccttggtg cggcagtttg tcgatgccgc cgtcaaacaa gccctgacca atctcgaatc
    31861 ccgtcctgcg cctgccggcg agatgaccgt cgttttgggc aacggttggc cgggcgtgtt
    31921 gctgcacgaa gcggtcggac acggtttgga aggcgatttc aaccgcaagg ggaccagcgt
    31981 cttttccggc cggatcggcg aacgcgtcgc cgccaaaggc gttaccgtcg tcgatcaggg
    32041 cgatattgcc ggccggcgcg gttcgctcaa catcgacgac gaaggcaacg aaacacgccg
    32101 caccgtattg attgaagacg gcatcttggt cggctatatg caggacgaaa ccaacgcccg
    32161 cctgacgggt acgcaatcga ccggcaacgg ccgccgccaa agctacgcct ccgtcccgat
    32221 gccgcgtatg accaacacct ttatggaaaa cggcagctac gagccggaag aaatcatcgc
    32281 gtccatcgac aagggcattt acgccgtcaa cttcggcggc ggacaggtgg acatcaccag
    32341 cgggaaattt gtcttcggcg cgtccgaagc gtggtgggtg gaaggcggca ggctgcaata
    32401 ccccgtcaaa ggcgcgacca ttatcggcaa cggccccgaa gtgctgaaac acgtttcgat
    32461 gataggcaac gataccgcgc tcgacagcgg tgtcggcgtg tgcggcaaag aagggcagag
    32521 cgtccccgtc ggcgtggggc agccgaccct gcgtatcgat gccgggctga ccgtcggcgg
    32581 cagcgcaatc tgacgcggaa tccggcacga tgccgtctga aaggttttca gacggcatcg
    32641 ccttattctt ttttccgatg tttgatattc ctaagcattc tttaacatta tcttacaagt
    32701 tcattaaaat accgcctttc gttaagctga tttctgatga actggcctta tttaatcgat
    32761 gccgtaccca aattcgccga tgcggcaaag ctgacgctgg aattgtccgt ttacggcgtt
    32821 gttttgtcgt tgctgttcgg cctgcccgtc gccgtggtaa cggcataccg catccgcccc
    32881 ttctacgcat tggcgcgcgc ctatatcgag ctgtcgcgca atacgcccct gctgatccaa
    32941 ttgtttttcc tatattacgg tctgccgaaa atgggcatca aatgggacgg tttcacctgc
    33001 ggcgtaatcg cgctggtttt cttgggcgca agctatatgg cggaagctgt ccgcgccggc
    33061 atcctcgccg tccccaaagg gcaggtcgga gcgggcaagg caatcggttt gagccgcttt
    33121 caagtgttcc gctatgtcga attgccgcag gtttgggcgg tcgccgttcc cgccatcggc
    33181 gcaaatatat tgtttttaat gaaagaaaca tccgtcgtca gcacggtcgg cattgcggaa
    33241 ttgttatttg ttaccaaaga cgtcatcggt atggactaca aaaccaatga agccttgttc
    33301 ctgctgtttg ccgcttattt gattatcctg ctgcctgttt ctttgttggc gcggcggatt
    33361 gaaaaccgcg tacggagtgc gaaatatggc gtttgaatgg ctgtttgaag gaaaaaatgc
    33421 ggcacggttg ggcgaaggcc tgctcttaac ggcacaaatt tctttaatct ctgttgcggc
    33481 ttcttgcgta ttgggcacgc tgttcggctt ggttttgcgt tcgcgcaacc ggcttgtccg
    33541 ctttgtcgga cggttttatc tcgaaaccat ccgaatcgtg ccgattttgg tgtggctgtt
    33601 cggcctgtat ttcggcctat ccgtctggac aggcatccat atcggcggat tttgggtctg
    33661 cgtctgggta ttttccctgt ggggcgttgc cgaaatgggc gacttggtgc gcggcgcact
    33721 ggaatcgatt gaaaaacacc aggtcgaatc gggtcttgcg cccggcttga gccgggggca
    33781 ggtattccgc tgcatcgagc tgccgcaaag catccgccgc gtattgcccg gcgcggtcaa
    33841 cctgttcacg cgcatgatca aaaccagctc gctcgcctgg cttatcggcg tgatcgaagt
    33901 cgtcaaagtc gggcagcaaa ttattgaaaa ctcgttgctg acgcagccca atgcttcatt
    33961 ttgggtttac ggcctgattt ttatgctgta tttcttctgt tgctggccgc tatctctact
    34021 ggcggcaaaa cttgaacaaa aatgggaaca ctgacatggc tttactgagc atccgcaagc
    34081 tgcacaaaca atacggcagc gtaaccgcca tccaatcctt agacttggac ttggaaaaag
    34141 gcgaagtcat cgtactgctg ggcccgtccg gctgcggcaa atccaccctc ctgcgctgcg
    34201 tcaacggttt ggagccgcac caaggcggca gcatcgtgat ggacggtgtc ggcgaattcg
    34261 gcaaagacgt ttcctggcaa accgcccggc aaaaagtcgg tatggtcttt caaagctacg
    34321 aactgtttgc ccacatgacc gtcatcgaaa acatcctctt aggcccggta aaggtacaaa
    34381 accgcgaccg tgccgaagca gaggcgcaag ccggcaaact gttggaacgc gtcggactgc
    34441 tagaccgcaa aaacgcctat ccgcgcgaac tttccggcgg tcagaaacag cgcatcgcca
    34501 ttgtccgcgc cctgtgcctg aatccggaag tcatcctgct ggacgaaatc accgccgcac
    34561 ttgaccccga aatggtgcgc gaagtcttgg aagtggtttt ggaactcgcc cgcgaaggga
    34621 tgagtatgct catcgtaacc cacgaaatgg ggttcgcacg caaagttgcc gaccgcatcg
    34681 tctttatgga caaaggcggc atcgtcgaat cgtccgaccc cgaaaccttt ttttccgcac
    34741 caaaaagcga acgcgcccgc caatttctgg caggtatgga ctactgattt accgcaaacc
    34801 gtccgaaccc ttttcagacg gcatttcata gggcagtgct ttatgtgtcg tcctgtgtgt
    34861 tgaaacatcc caagcaaaca ttcaaaaagg agatttccaa tgaaattgaa cgccaaactc
    34921 aaagcccttt tggcttccgc cgccatcgcc gtcggtctga ccgcctgcgg gggcggctcc
    34981 ggcgatgccc aatcttcaca aagcagcggt gcggcaaccg ttgccgccat caaagaaaaa
    35041 ggcgttatcc gcatcggcgt attcggcgac aaacccccgt tcggctatgt tgacgcaaac
    35101 ggcaaaaacc aaggctttga cgttgaaatc gccaaagacc tggccaaaga cctgctcggc
    35161 agccccgaca aagtcgaatt cgtcctgacc gaggccgcaa accgcgtcga atacgtccgt
    35221 tccggcaaag tcgacctcat cctcgccaac tttacccaaa cccccgaacg cgccgaagcc
    35281 gtcgatttcg ccgatcctta catgaaagtg gcgttgggcg tggtttcccc caaaaacaaa
    35341 ccgattaccg acatggcgca attgaaagac caaaccctgc tggtcaacaa aggcaccgcc
    35401 gccgacgctt tcttcaccaa aagccatccc gaagtcaagc tgttgaaatt cgaccaaaac
    35461 actgaaacct tcgacgcgct gaaagacggt cgcggcgtag cactcgcgca cgacaacgcc
    35521 ctgctgtggg cgtgggcgaa agaaaacccg aactttgaag tcgccatcgg caatctcggt
    35581 cctgccgaat ttatcgcccc cgccgttcaa aaaggcaacg ccgacctcct gaactgggtc
    35641 aacggcgaaa tcgccgccat gaaaaaagac ggccgtctga aagccgccta tgaaaaaacc
    35701 ctcttgcccg tgtatggcga gaaagtcaaa ccggaagcat tgttggccga ataagtttgt
    35761 ttgacaagaa aatccgtatc cgcatttgaa ggcgggtgcg gatttttttc agccgccgaa
    35821 aatattgcaa attcaaagga aaaatcaact atctcatcgt tttatttatt ttattattct
    35881 tgcgcgtgcg gaaatcaagt tcgtccggtt ttgcctgttt ttgaatttcg ggaaacttcc
    35941 aaatcgtcat tccagataaa acgcttcagc cttatgtttc cggatgaccg ttttcgcggg
    36001 atgacggcgg agcgtttctg ttattcggat aaattcctac ggtattgaaa cggcgataag
    36061 gtgttttcct ttattatata atttcaatgc aaaatgatat tttttgaaca agcgtgccgc
    36121 aaagccggaa aaaatgttca agccgcatcg gcggcagacg ggacgaaaaa tgacgtttga
    36181 cgaatggttg ggcttgtcaa aactgcctaa aattgaagca aggatgctgc tgcaatatgt
    36241 ttcggaatat acgcgcgtgc agttgttgac gcggggcggg gaagaaatgc cggacgaaat
    36301 ccgacagcgg gcggacaggc tggcgcaacg ccgtctgaac ggcgagccgg ttgcctatat
    36361 tttaggtgtg cgcgaatttt atggcagacg ctttacagtc aacccgaacg tgctgattcc
    36421 gcgccccgaa accgaacact tggtcgaagc cgtattggcg cgcctgcccg aaaacgggcg
    36481 cgtgtgggat ttggggacgg gcagcggcgc agttgccgta accgtcgcgc tcgaacgccc
    36541 cgatgcgttt gtgcgcgcat ccgacatcag cacgcccgcc cttgaaacgg cgcggaaaaa
    36601 tgcggcggat ttgggcgcgc gggtcgaatt tgcacacggt tcgtggttcg acaccgatat
    36661 gccgtctgaa aggcaatggg acatcatcgt gtccaacccg ccctatatcg aaaacggcga
    36721 taaacatttg tcgcaaggcg atttgcggtt tgagccgcaa atcgcgctga ccgacttttc
    36781 agacggccta agctgcatcc gcaccttggc gcaaggcgcg cccgaccgtt tggcggaagg
    36841 cggttttttg ttgttggaac acggtttcga tcagggcgcg gcggtgcgcg gcgtgttggc
    36901 ggagaatggt ttttcgggag tggaaatcct gccggatttg gccggtttgg acagggttac
    36961 gctggggaag tatatgaagc atttgaaata attgtttgca aaattggcgg attttccgtt
    37021 attgggttat gatgtgtttt gatatgtaaa catttatagg tttggagcga taaacaatga
    37081 atgcagtcgt agttgccgta atcgttatgc tggtgctgtc gctgtcgcgc gtgcacgtgg
    37141 tattgagcct gacggtcggc gcgtttgtcg gcggcgcggt ggcgggtatg ccgctgcaaa
    37201 acattgccga tgcggcggga caggtcagtc aggcggggat tatccccgtg ttcaacaaag
    37261 gtttggaagg cggcgcgaag attgcgcttt cttatgcgat gcttggtgcg tttgcgatgg
    37321 cgattaccca ttccggcctg ccgcagcagc ttgccggcgc ggtcgtccgc aagctgaacc
    37381 ggggcggtat gcccgacagc gtgcgttcgg gcgagggcgc ggtcaaatgg ctgctgcttt
    37441 ccatcatcct tgtgatgggc attatgagtc agaacgtcat acccatccac atcgccttta
    37501 tcccgatgat tgttccgccg ctgcttttgg tgtttaaccg cctgaaaatc gaccgccgcc
    37561 tgattgcgtg cgtcatcact ttcgggctgg ttacgactta tatgttcctg ccttacggct
    37621 tcggcgcgat ttttttgaac gaaatcctgt tgggcaacat ccattccgcc gcgccgcagc
    37681 ttgatgtgaa aaacatcaac gtgatggcgg caatggcgat tcccgcattg ggaatgctgg
    37741 ccggactcct gctggcgttt gtccactacc gcaaaccgcg actgtaccaa agcaacaatg
    37801 ccgatacggc gggcaacgcc gatgcggcaa accgtccgca gccgtccgcc taccgcagcc
    37861 tggttgccgc cgccgccatc gccgtatgct ttgccataca gttgatgtat gaagattcgc
    37921 tggtgttggg cgcgatgctc ggtttcgccg tatttatgat gttgggggtc attaaccgcg
    37981 acaaggcaaa cgacgtattc ggcgaaggca tcaagatgat ggcgatggtc ggcttcatta
    38041 tgattgccgc gcagggtttt gccgccgtga tgaatgcgac cgggcatatt cagccgctgg
    38101 tggaaagcag tatggcgata ttcggcaaca gcaaaggtat ggcggcgttg gcgatgctgg
    38161 tggtggggct tttggtaacg atgggcatcg gttcgtcctt ttccactttg ccgattattg
    38221 ccgcgattta tgtgccgctg tgtaccggtt tgggcttttc gccgcttgcc accgccgcca
    38281 ttgtcggcac ggcgggggcg ttgggcgatg ccggttcgcc cgcgtccgat tccacgctgg
    38341 gcccgacgat ggggctgaac gccgacgggc ggcacgacca tatccgcgat tccgtcatcc
    38401 cgaccttcat ccactacaac atcccgcttc tgattgccgg ctggattgcc gcgatggtgc
    38461 tgtaaatgga cgcggttcaa gagttggaac gccgtattgt cgaactggaa atccaaaccg
    38521 cgcttcagga ggacgtaatc tccggtctga acgcgatggt ggcggaattg cggcagacgt
    38581 tggatttgca gcaggctcag ttgaggctgc tgtatcaaaa aatgcaggac aggaatcccg
    38641 acgcgcaaga gccgtattcc ctgcgcgacg agattccgcc gcattattga tgcgccgccg
    38701 tatccggatt tccttaaata aaggctgtgt ttgaatattc cgccggtgca accctaagat
    38761 gtaaccttaa tcagtcggac aaaatgcctt ttatccgatg aaaccggttc ttgtccgaac
    38821 ggcgatgagc ggcttccccc gctgcaaaca gataaaacca accgggtatt caaacacagc
    38881 caaaaaaagc cgtccgaacc caaaggggac agacggccaa acacattacg gggaaaacgt
    38941 cttactcaat gagtctgcga aacagacatt gctaaaacaa ctgcaattat aaggtaagtt
    39001 tatttttttg caaagaaatg taaattttta aatttttaaa accgttccgc cgcatccgtt
    39061 cggatgccgt ctgaaacggc ggcagggttt taccgtgccg cgccgcaaac cgggcattcg
    39121 gggttgcgcg gcaggtcgaa atattgccag cccccttcca aggcacggta aaccgccagc
    39181 ctgccgtgcg acggttcgcc cgcttccagc aggattttca gagcctccgc cgcctgtgtg
    39241 cagccgacga tgccgaccag cggcgagagc acgccgaaga gggaacagat gccgtctgaa
    39301 gccgatccgc cgtcgaacag gcaggcgtaa cacggcgagt cgggcaagtc gggacggtat
    39361 gcggcaagct gtccttcaaa acgtaccgcc gcccctgaaa ccagcggtgt tttcgtttgc
    39421 acgcaggcac ggttgaccgc ctgccgcgtg gcgtagttgt cgcagcagtc taaaacgatg
    39481 tcggcggttt gaaccaaacc ggtcaggcgg cagccgtcga gtttttcgtt gacggcgcgg
    39541 acatcgacag tatggttgat gcgtttcagg cggcctgcca aggtttcggc tttaggcttg
    39601 ccgacatcgc cctcgtcaaa tgcgacttgg cgttgcaggt tgtgcagctc gaccgtgtcg
    39661 gaatcggcta tggtcagcgt gccgacgccc gaggcggcga gatagggcag ggcggcggcg
    39721 cccaatccgc cgcagccgac gaccaaaata tgcgcggcgg aaagtttctg ctgcccttcg
    39781 atgccgattt cgtccaagag gatgtggcgg ctgtaccgca gcaggagtgc gtcgtcgttg
    39841 tcgtgttcgg tcgcggtcat gatgatgttc ggaaaaaaca gttgcgggcg attgtaacgc
    39901 cgccgtcggg cggcgttcaa cttcagacgg catttcggga catgagtggt taaagtgtga
    39961 acggtttggc acggatgcgg catttgaggt acatttacag tatttacggc aatgagagga
    40021 aaatcatgca actgcatatc ctgaacaatc cgaaggacgc ggctttggcg gcggacgcgg
    40081 aatttttgaa acagtccctg ttcaacctcc tgcacgaaga agcctcgccg ttggttgtcg
    40141 aaacggtcaa actcttgtcc acttccgacg acagcgcggc attgattgaa aaagtattgc
    40201 cgcaattgga cgaacggcag acctacgatc tgacgcttgc ctgcgggctg ttcgcccaga
    40261 ttctgaatat cgccgaagac gtgcaccacg aacgccgccg ccaaatccac gaagatgccg
    40321 gccacaacgc agcagaaggc agcctgacgg aaaccgtccg caggctcaaa gcggggaaag
    40381 ccgacggcaa atcggtgcag cggcagttgg acaatacgtc cgttaccgcc gttttgaccg
    40441 cgcacccgac cgaagtgcaa cgccaaaccg ttttgaattt caaccgccgc atccgcgcgc
    40501 tgctgccgca acgcgaacgc tgcaccaatg ccgacgcgct ggcacggctg cgccgcgaaa
    40561 tcgacaccgt gcttctgggc ttgtggcaga ccagcgaaac gcgccgccac aaactcagcg
    40621 tcaacgacga aatcaacaac ggcgtgtcca tcttcccgat gagctttttt gaagccctgc
    40681 ccaaactcta ccgcaagatg gaacacgact ttcagacggc ctatcccgat gtccgcgttc
    40741 cgaacatcct taaaatcggc ggctggatcg gcggcgaccg cgacggcaat ccgtttgttt
    40801 ctggcgaaac cctgcgcttt gccttccgcc gccacgccga tgccgtgttc cgtttttacc
    40861 gcagcgaact cgacaaactt taccgcgaac tgccgctttc catccgccgc gtcaaagtca
    40921 acgacgacgt aatggcactg gcagccctct cgcctgacga agaaatcgcc cgcaccgaag
    40981 aaccttaccg ccgcgccatt gcctacatta tggcgcgcgc tatgggcaaa gcgcgctcgc
    41041 tcggtttggg catgggctgc aaattcggct ttctcgagcc ttatgcttcg gcccaaaaat
    41101 ttctcgatga tttgaaaaaa ttgcaacgtt cccttatcga caacggcagc cgcctgcttg
    41161 ccgaaggccg tttggcagac ctcatccgtt ccgtgtccgt gttcggcttc cacatgatgc
    41221 cgctcgacct gcgccaacac gcaggcaaac acgccgatgt ggttgccgag cttttccaac
    41281 acgcaggctt ggaagactac aacagcctga acgaagagca aaaacaagcc gccttgttgc
    41341 gcgaattggg ccaccaacgt ccgctgtaca gcccgttcat cacatacagc gaccataccc
    41401 gccgcgaatt ggcgattttc aacgaagcgc gcaaaatcaa agacgaattt ggcgaagatg
    41461 ccgtaacaca aagcattatt tccaactgcg aacaacccgg cgacctgctc gccttggcat
    41521 tgctgctgaa agaaagcggc ctgttggcgg tggaaaacgg caaaccgcac agccgcatca
    41581 acatcgtgcc tctgtttgaa accatcgaag cgctcgaaaa tgcctgtccg gtcatggaaa
    41641 ccatgttccg cctcgactgg tatgatgccc tgctcgaaag ccgcggcaac atccaagaaa
    41701 tcatgctcgg ctattccgac tccaacaaag acggcggcta cgttaccagc tcatggtgtc
    41761 tgcatcaggc ggaattgggc ttggtcgaac tcttcaaaaa atacgatgtc cgtatgcgct
    41821 tgttccacgg tcgaggcggc agcgtcggac gcggcggcgg cccgtcttac caagccatct
    41881 tggcgcaacc cgcaggcagc gttgccggac aaatccgcat taccgagcaa ggcgaagtca
    41941 tcaccgccaa atacgccgac cccggcaacg cccaacgcaa cttggaaacc ttggttgccg
    42001 cgactttgga agccagcatc ctgccggata aaaaagaccc tgatgccaaa ctgatgcagg
    42061 cattgtcgga cgtatcgttc aaatactacc gcgaactgat tacccacccc gacttcatcg
    42121 actacttcct gcaaaccagc ccgattcagg aaatcgccac cctcaacctc ggcagccgtc
    42181 ccgccagccg caaaaccctg gcgcgaattc aggatttacg cgcgattccg tgggtattct
    42241 cctggatgca aaaccgcctc atgctgccgg cttggtacgg tttcggcagc gcggtggaaa
    42301 ccttgtgcga aggcagcccc gaaaccctcg ccgccctgcg cggacacgcc caaaacaacc
    42361 ccttcttcca agcgatgctc tccaatatgg aacaagtgat ggcgaaaacc gacatcacct
    42421 tggcggaaaa ctacgccggt ttgagcgaat cacccgaaaa ggcaaaagtc atcttcggca
    42481 tgattaagga agaataccga cgcagccgca aagccctgct cgacctgctg caaaccgaag
    42541 aacttttgcg cgacaaccgc agcctcgccc gttcgctcgc tttgaggatt ccatacctga
    42601 acgcgctcaa cggtttgcaa gtcgccatgc tcaaacgcct gcgcaaagaa cccgacaatc
    42661 cgcacgccct gctgatggtg cacctgacca tcaacggcgt ggcgcaaggt ttgcgcaata
    42721 ccggctgata gtgccgcatc ggggcaaaat gccgtctgaa cgcctttcag acggcattta
    42781 cctgaccgca cttgcagaga aacaccgatt gttttaaagt gaacggcagt gatatgttga
    42841 aagacgacca atgaaaatta ccgttatcgg cgcaggttcg tggggtacgg cgctcgccct
    42901 gcatttttcc caacacggca accgcgtatc cctgtggacg cgcaacgcag accaagtccg
    42961 ccaaatgcag gaagcgcgtg aaaacaaacg cggactgccc ggcttttcct ttcccgaaac
    43021 cttggaagtg tgtgcggatt tggcagaggc gctcaaagac agcggacttg tccttatcgt
    43081 aacctccgtt gccggattga gaagcagcgc agagctgctc aaacagtacg gcgcgggaca
    43141 cctccccgtc ctcgccgcct gcaaaggctt cgagcaggat acagggctgc tgaccttcca
    43201 agtcttgaaa gaagtattgc ccgacaataa gaaaatcggc gtactttccg gtccgagttt
    43261 tgcacaggaa ctcgccaaac aactgccctg cgccgtcgtc ctcgcctccg aaaaccaaga
    43321 gtggattgaa gaactcgtac cgcagctcaa cacgaccgtc atgaggcttt acggcagtac
    43381 cgacgttatc ggcgtggcag taggcggttc agtcaaaaac gtcatggcaa tcgctaccgg
    43441 actctccgac ggactcgaat acggactcaa cgcccgtgcc gcactggtta cgcgcggact
    43501 ggccgaaatc acccgccttg cctccgcaat gggcgcgcag cccaaaacca tgatggggct
    43561 tgccggcatc ggcgatctca tcttgacctg taccggtgca ctttcgcgca accgccgcgt
    43621 cggcttgggt ttggcagaag gcaaggaact gcatcaggtg ctggtcgaaa tcgggcacgt
    43681 ttccgaaggg gtcagcacca tagaagaagt cttcaatacc gcctgcaaat accaaatcga
    43741 tatgccgatt acccaaaccc tgctgcaact catccgcaaa gaaatgaccc ctcagcaggt
    43801 cgtcgaaagg ctgatggaac gcagcgcgcg tttcgaataa acaacaaaca gatgccgtct
    43861 gaacccccag acggcataca gacaggtaag gtcatgaaac aaaatatcga aaaactcgaa
    43921 agcagcgttt atacgttggt acaaaaattc gaaaccctcg tcagcgaaaa ccgccgcctc
    43981 aaagaaaccg tcgccgaact cgaacgggcg cacgagcggc aaaaactcga acacgagacc
    44041 gccgtcgacg aactcagcga agccctgctc gtccaagtcg gcaaactcaa agaagacctg
    44101 caaaacaaaa tcgacagcct gacggaagaa aatgcacgat accgcgccct gctcgaacag
    44161 agcagggaga aaatcagcgc actggcagcg cgcctccccc aacggcagga aacgcagcaa
    44221 taaggattaa aggatgaaca tcgaacaagt ctacatcgaa gtcatgcacg cccggctgac
    44281 cgtcaacacg ccggcagaag aaaaagacac actgttgcag gcagtcggaa tgctcaacgg
    44341 caaagccgaa gccatccgcg aaggcggccg cgtcgcggac agcgaaaaaa tcgtcattat
    44401 ggccgcgctc aacgtcgtcc acgacctatt gaaaacctcc ctgaacggcg gcgatttggc
    44461 aatcggcgat tttgcgcgta aaataaccga tatggacaac gcctgccaaa aggcactatc
    44521 ccgcttgggg caggaataac ccctttttcc ctgcggtgtc cgcgaaggca tatattcctt
    44581 tgaaccaata agttcgctta aggttgccgg gagcagtagt gggcgcgagc gtccttttgc
    44641 ggacgcaccc gaaactaccc ggggcagccg ccttgtcagc aaggttcaag cggattcggc
    44701 cgaaacggct ctcgcgggga ttcccattca aaaccgcatc gcaacgatgc ggtttttcac
    44761 attggccgcc gtcccgcccg aaaaagcagt atgcagtctg ccgaaatccg gaaaatcctt
    44821 ccgtaacgcg gtaatgcgat tgacaaaatc aaatcctgtc tttaaaatcc ataactttct
    44881 atatctatct ggatttccac tatgaaaacc ttttcagcga aacctcacga ggtgaaacgc
    44941 gaatggttcg tcatcgatgc ccaagacaaa gtcttgggtc gcgttgcaac cgaagtcgcc
    45001 agccgtctgc gtggcaaaca caaacctgaa tacacccccc acgtcgatac cggcgattac
    45061 atcatcgtca tcaatgcgga caaactgcgt gtaaccggtg ccaaattcga agataaaaaa
    45121 tacttccgcc attccggttt tccaggcggc atctacgagc gcactttccg cgaaatgcaa
    45181 gatcaattcc cgggccgcgc tttggagcag gctgtaaaag gtatgctgcc caaaggtccg
    45241 ctgggttacg ccatgattaa aaaactgaaa gtgtacgctg gtgcggagca tgcccatgct
    45301 gcgcaacaac ccaaagtttt ggaactgaaa taataaggac acgacatgaa cggtaaatac
    45361 tactacggca caggccgccg caaaagttca gtggctcgtg tattcctgac taaaggtacg
    45421 ggtcaaatca tcgtaaacgg ccgtcccgtt gacgaattct tcgcacgcga aaccagccgt
    45481 atggttgtcc gccaacctct ggttctgact gaaaacgccg aatctctcga catcaaagta
    45541 aatgttgtcg gcggcggcga aaccggccag tccggcgcaa tccgccacgg tattacccgt
    45601 gccctgattg attttgacgc tgctttgaaa cctgccttgt ctcaagccgg tttcgttacc
    45661 cgcgatgcgc gcgaagtcga acgtaaaaaa ccgggtctgc gcaaagcacg ccgtgcaaaa
    45721 caattctcca aacgttgatt ttggaaattc aaaaaaccct gcttatcgca gggtttttta
    45781 tttgtaacag gcggtttccc attggtaatc taaagattac ggattgggca aaaatcaaaa
    45841 acagcaccag tatcgactac acggacgaac tgaccgtcag ggatggctgt tactggagag
    45901 gacatcgtat ttctactttg taaaataact tttaatttat ttagagttat tgaaaaaata
    45961 gaacggctat ttcaactagc ctataaaaat ggcaatctat tagggttagc ataggtgtca
    46021 ccgccgccgt tcaagccgat cagtaaagga acagtttcct agtttttagc taacctgtat
    46081 tatataatgg ggagaatatc ttaaataacc aagcatcctt aatcataatt tagagaaatt
    46141 attttttcag agtctaaccc ttttaatatg attaacttat taactttatc tatttcccag
    46201 tctgatatta tatcggtaga tactgaattc tctcttatta aatttttatc tgtaataatt
    46261 gcacccaact catgaataat acaaattttt tgattatcca ataaataaat atcaaaaact
    46321 attccatcta tatttagtat gttaactttt gaattttgta tagaaataca aaaaacgact
    46381 gaatcaaatc cgataaaaat atttttatca tcaagcatta atacttttgg gggaatactt
    46441 atgcaagaaa cagctaatcc aatacctata ttgaattctt tataaaaaga aagaataaaa
    46501 catcgataaa tctcttttcc tccctcttgt agaataatat atttttgata ggtgtttatt
    46561 aaatagtctt ctaactctct tatggaatta taaggatatt tcgttaatct cattattttg
    46621 ttccatgtct aaaacgtcct gttgatccat atatttcacc cgttatgagt ctgttcccat
    46681 ctcatatagt ttcaagttta acaacccctt gaggctatgt taaaagagtt tttctaataa
    46741 ctattccgtt ttcttaaata taaaaaaata gaaaaatagc ccgggtatag tttgaacaag
    46801 ggaaatcaga ccggttccaa ctcacttaat ccgggcaagt ccgcgaagcc gcgttcacgt
    46861 atgatttggc aaaagttgtt cagataactt ttatctgtgt cttcagtgcg gatggcggca
    46921 tacagtttgc tttgcagtcc gtcggcagta atttggcggt ggacgacata gcctttttca
    46981 aggtagggca tgactgtcca atagggaagg gcggcaatgc cacgtctgct ggcaaccagt
    47041 tggataatgg cgatggtcag ctcgctgtgc cggcgcggcg ggttgatgtt tttcggaatc
    47101 aggatttttt tgggtaaatc cagcatttca tcgggaacgg gataggtaat cagggtttcc
    47161 ccgataaagt cttccgccgt ccaaacgttt ttggcggcaa gcggatggtc cggggcgcaa
    47221 atgccgacca tttcgtaggc aaacagtggg tggaagctga tgctgctttg tttttcggct
    47281 tcggagacaa tggcgaggtc ggcgcggtgt tgcagcagca gcccgacggg atccgcttgg
    47341 aatcctgata cgatatccag ttcgacttgg ggccacatcg ggcggaattc gcccatggcg
    47401 ggcatcagcc agtcgaaaca ggtatggcat tcgacggcaa tccgcagctc tcccgcctcc
    47461 ccttccgtga ttcgcgccaa atctcgttct gcaacagcaa cttgaggtat cagttcgtgg
    47521 gcgaggcgca gcagcctttc gcccaccggg gtaaagcgca ggggcgtgga tttgcgttcg
    47581 aacagcggcg tgccgtagtg gttttcgagc atacggatct ggtgggaaag ggcggattgg
    47641 gtaaggaaaa cccgtttggc ggcaagggag acgctgccgg tttcttcaag tgccagcagg
    47701 gttttgaggt ggcgcaattc gataatggaa tccatggggc ttcagacggc atattgaacc
    47761 ggcgcatatt aaaataattc atatgcgggt gcaaatcgga aaaatgggcg tttcgggcat
    47821 ttgcggttag aatgcccgct tgtttggaaa tgacaggaaa aatatggttt tgcgcagaga
    47881 ttttttagcc tggtgcgacg aaacgttgca gacggcatcg tttaaagatt acgcccccaa
    47941 tggtttgcag gttgaaggga gggaatatat cgggaaaatc gttacgtcgg taacggcaag
    48001 cagggcagcg attgattttg ctgtggagca gaaggcagat ttgcttttgg tacatcacgg
    48061 tatgttctgg aaaaacgagc tgccgaccgt taccggttgg aaaaaagaac ggattgccgc
    48121 actgttacgg cacgacatca atatggcagg ctaccatctg cccctggatg tgcatcccat
    48181 actgggcaac aatgcccaac tcgccgacag attgggtttt gcgacagaaa aacggttcgg
    48241 cgaacaaaac ctgctcaact cgggcagcct gaaacaagcc aagacactcg gcgcattggc
    48301 ggcgcatatt gaaacagttt tgcaacgtaa gcctgtcgcc atcggcaatc ccgaacgcga
    48361 aatccgacgg gttgcatggt gtaccggcgg tgcacagggc ttttttcaga cggcaataga
    48421 tgaaggcgtc gatctgtatc tgacgggtga aatttccgaa gcccaatacc acctcgccaa
    48481 tgaaacgggt acggctttca tttcggcagg gcatcacgcg acggaacgtt acggcgtacg
    48541 cgcgctggca gaatcggcgg cagaggtttt cgggttggaa gtgtgccatt ttgacgaaaa
    48601 caacccggct tgagtcttga gaaatatcat aaaactttac cttattttaa ataatgcttt
    48661 gagtatcact gcaaactggg taaaattgca atgtttaatg gctcggcatt cccaaaatat
    48721 taggacgact gaataatgga taatcaagaa atcaacaacg gccgccgccg tttcctgaca
    48781 cttgcgacct gcggcgcggg cggagtggca gcattgggtg tggcaacgcc gtttgtggcc
    48841 agttttttcc cttcggaaaa agccaaggcc gccggtgctg ccgtcgaggt ggatgtcagt
    48901 aaaatcgaag cgggtcagct gctgaccgcc gagtggcaag gcaaaccgat ttgggtgctc
    48961 aaccgtacag atcagcagct taaagacctg aaaggcctga acggcgaact taccgatccc
    49021 aattccgatg cggaacagca gccggagtat gctaaaaacg agacccgttc gattaagccg
    49081 aacatccttg tcgccatcgg tatctgcacc catttgggct gctcgcccac cttccgtccc
    49141 gacattgccc ccgccgattt gggcgcagac tggaagggcg gcttcttctg cccgtgccac
    49201 ggttcgaaat tcgacttggc cggccgcgta tataaaggtg ttcctgcccc gaccaacctg
    49261 gttgtcccgc catataaata cttgagcgac acaactatct tggtgggcga agactaagaa
    49321 taaggaacga taattatggc aaaccaaacc aatagcaaag caaaagcatt gttaggctgg
    49381 atggacgctc gcttccccct gtctaaaatg tggaatgagc atttggcgca atactatgcg
    49441 ccgaaaaact ttaatttttg gtactatttc ggctctttag ccctgctcgt cctcgtgatt
    49501 caaatcgtca gcggtatttt cctgacgatg aactacaaac cggacggcaa cctgaatgcc
    49561 taccatctgc ctgctgcctt taccgcagta gagtacatca tgcgcgacgt gtccggcggt
    49621 tggattatcc gctacatgca ctccaccggc gcatctttct tcttcattgt tgtttatctg
    49681 cacatgttcc gtggtttgat ttacggttca tataaaaaac cgcgcgaatt ggtgtggatt
    49741 ttcggttctt tgatcttctt ggcattgatg gcggaagcct ttatgggcta cctgctgcct
    49801 tggggtcaga tgtccttttg gggtgcgcag gtgattatca acctgttctc cgccattccc
    49861 gtcatcggtc ctgatttgtc cacttggatt cgcggcgact tcaacgtttc cgacgttacc
    49921 ctgaaccgct tcttcgccct gcacgttatc gccgtaccgc tggtattgct cggtttagtc
    49981 gtggcacaca tcatcgcact gcacgaagtg ggctctaaca accccgacgg tgtggaaatc
    50041 aagaaaaaca aagatgaaaa cggcattccg cgcgacggca tcccgttcca cccttactac
    50101 actgttaaag acatcctggg cgtcgttgta ttcctgattg tcttctgtgc cgtattgttc
    50161 tttgcccctg aaggcggcgg ctacttcctc gaagcgccga acttcgatgc ggcaaatgcg
    50221 ctgaaaacac ccccgcacat tgcgccggta tggtatttca caccgttcta cgccattttg
    50281 cgtgcgattc cttccttctt gggaacgcag gtttggggtg taatcggtat gggcgcggcg
    50341 gtcgtgttga ttgccctgct gccttggctg gacaggggcg aggtgaaatc cgtccgttac
    50401 cgcggtccga ttttcaaaac cgcattggtc ttgttcatca tttcctttat cggtttgggt
    50461 attttgggcg cgatggtggc aaccgatacg cgtactttgg tcgcgcgcat cctgtccttc
    50521 gtctatttcg cattcttcct gggtatgccg ttctatacca agctggataa aaacaaaccg
    50581 gttcccgaac gcgtaaccat gagcaccgcc aaacaaaaaa tcatgttctt tgtttacgtc
    50641 ggcattactg ccgtgggtgc ttacctgttt gcaaccaaca tctgatgagg gcagtcgaaa
    50701 tgaaacaagc tatgaagaat tggtttgctg ccttgctgtc ggcagtaccc atgagtgcgg
    50761 catttgcttc gggcggtcat gcacactatg agaaagtcga tattgatttg cgcgaccaag
    50821 taagcttgca gcgcggcgca caaatcttta ccaactactg tttgtcctgc cactcggcaa
    50881 gcggtatgcg tttcaaccgt ttgaaagaca tcggtctgac tgatgaagaa atcaagaaaa
    50941 acctgatgtt taccaccgat aatgtcggcg atgtcatgca ttcggcgatg aacccgaaag
    51001 atgcggcaaa atggtttggt gctgctccgc ccgatttgac attgattgcg cgttccaaag
    51061 gtgcagacta cctttacgcc tatatgcgcg gcttctataa agatccgacc cgtccgagcg
    51121 gctggaacaa taccgtattc gataaagtcg gtatgcccca cccgttgtgg gaacagcaag
    51181 gtgttcaggc agtcgagttg gatgccaaag gtcagccggt tatggtaaaa gacgaacacg
    51241 gcgagatgaa gcctaagctg tattgggaat ctaccggcct gcacagccgc cgcctgccga
    51301 acggcaaagt catccaaaaa gagtacgata cttatgtacg cgatttggtc aattaccttg
    51361 tgtacatggg tgaacctgca caactgcaac gcaaacgtat aggctatgtc gtgatgattt
    51421 tcttgttggc ggttatgctg cctttggcat acttcctgaa aaaagaatat tggaaagacg
    51481 tacactaagc gtttggaaca aaagggcaaa tcctttaggg tttgcccttt ttgcatgctt
    51541 ctgccctttg ggaaaatcag cacccggatg ccgtctgaac atagctgcaa catttcagac
    51601 ggcatcattc taaaaatgtc agacatcggg aacttaatca gggtttgtgg cagattgata
    51661 ttgaattagg aaaaggtctg aaaccgcaag cagcatggta aggcgatgtc gaacaggttt
    51721 aaagccaatc cgctatattt tttggagctg caccgtgcaa acaggtagaa tggcggcggt
    51781 tttgtccggt gtgaaaatgt ttgaatatta ggatgattga tggagctgat gactgttttg
    51841 ctgcctttgg cggcgttggt gtcgggcgtg ttgtttacat ggttgctgat gaagggcagg
    51901 tttcagggcg agtttgccgg tttgaacgcg caattggcgg aaaaggcggc aagatgtgat
    51961 tttattgaac aggcacacgc agaaattgca tcagaattgg ccgctttgga tgggaaatac
    52021 gagcatttgc aggacgaaaa ttatgctttg ggcaaccgtt tttccgcagc cgaaaagcag
    52081 attgcccatt tgcaggaaaa agaggcggaa tcggttcggt tgaagcagtc gtatatcgat
    52141 ttgcaggaaa aggcgcaggg tttggcggtt gaaaacgaac gtttggcaac gcagctcggg
    52201 caggaacgga aggcgtttgc cgagcaatat gcgctggaac gccaaatccg ccaaagagtc
    52261 gaaaccgatt tggaagaaag ccgccaaact gtccgcgacg tgcaaaacga cctttccgat
    52321 gtgggcaacc gttttgccgc cgccgaaaaa cagattgcct atttgcagga aaaagaggcg
    52381 gaagcggagc ggttgaggca gtcgcatacc gagttgcagg aaaaggcgca gggtttggcg
    52441 gttgaaaacg aacgtttggc aacgcaaatc gaacaggaac gccttgcttc tgaagaaaag
    52501 ctgtccttgc tgggcgaggc gcgcaaaagc ttgagcgatc agtttcaaaa tcttgccaac
    52561 acgattttgg aagaaaaaag ccgccgtttt accgagcaga accgcgagca gctccatcag
    52621 gttttgaacc cgttgaacga acgcatccac ggtttcggcg agttggtcag gcaaacttat
    52681 gataaagaat cgcgcgagcg gctgactttg gaaaacgaat tgaaacggct tcaagggttg
    52741 aatgcgcagc tgcacagcga ggcaaaggcc ctgaccaacg cgctgaccgg cacgcagaac
    52801 aaggttcagg gcaattgggg cgagatgatt ctggaaacgg ttttggaaaa ttccggcctt
    52861 cagaaagggc gggaatatgt ggttcaggcg gcatccgtcc gaaaagagga agacggcggc
    52921 acgcgccgcc tccagcccga cgttttggtc aacctgcccg acaacaagca gattgtcatt
    52981 gattccaagg tttcgctgac ggtttatgtg cgctacacgc aggcggcgga tgcggatgag
    53041 gcggcacgcg aactggcggc gtatatcgcc agcatacgcg cgcatatgaa aggcttgtcg
    53101 ctgaaggatt acaccgattt ggaaggtgtg aacacattgg atttcgtctt tatgtttatc
    53161 cctgtcgaac cggcctacct gctggcgttg cagaatgacg cgggcttgtt ccaagagtgt
    53221 ttcgacaaac ggattatgct ggtcggcccc agtacgctgc tggcgacttt gcggacggtg
    53281 gcgaatattt ggcgcaacga gcagcaaaac caaaacgcgc tggcgattgc ggacgaaggc
    53341 ggcaagctgt acgacaagtt tgtcggcttc gtacagacgc tcgaaagcgt cggcaaaggc
    53401 atcgatcagg cgcaaaacag ctttcagacg gcattcaagc aacttgccga gggacgcggg
    53461 aatctggtcg gacgcgccga gaaactgcgt ctgttgggcg tgaaggcagg caaacagctt
    53521 caacgggatt tggtcgagcg tgccaacgaa cagacggcat ttgctttggg caaaccttcg
    53581 gaacaggaag cggctgacga agccgaatag gaaaggcgga tgcgtttcag acggcatcgg
    53641 ggcaggggat gccgtctgaa gactttggaa agtcggttag aaaaacggat tgtgccgttt
    53701 ttcgtgtccg atggaagtca tacgcccgtg tccggcgaca acttgcacgg tttcggggaa
    53761 ggcgaataat ttgttgcgga tattgttgat taagtcggcg tggttgccgc gcggaaaatc
    53821 ggtcctgcct atggtttcgt aaaacagcac gtcgcccgca atcagcagct ccgcttcggc
    53881 acaatagaaa accacgtgtc cgggcgtgtg ccccggaata tgcagcactt ggaaggcata
    53941 gcgtccgacc gtgagcgttt cgccttcttc gagccaacgg ttcggcgcaa aggcgggcga
    54001 gacggggaat ccgtattgcg cggtggtttg cggcagcgat tggagcagga attcatcttc
    54061 gcggtgcggg ccgaggacgg ggactttatg cgtttccagc atctcgacca cgccgcccgc
    54121 gtgatcgaga tgaccgtgcg tcagccagat tgccgtgagc gtgagtttgc ggtttgccaa
    54181 cgcttgcagc aggaacggca cgtcgccgcc gacatcggtc aggacggctt cgccgctttc
    54241 gtcgtcccaa atcagggtgc agttttggcg gaagggggta acggggagaa tttcgtaacg
    54301 tagggtcatc ggcgtgttcc taaaacggtt tttcagacgg catcgggttt gccgtttgtt
    54361 ttatggcggt ttgccgcctg ttttgatatt gggggaatca ggtgattaag aagataatcg
    54421 gcggcatcat accgattttt acggcggttt tcatccctgc atcggcaggc gcggcggatt
    54481 tgatgctggc gcaggaatac aaagggcagg acattgccgg ctgggcgatg agcgagaagc
    54541 tcgacggcgt gcgcgcgtat tgggacggaa agcacctgat aagccgtcag ggttatgcgt
    54601 ttacgccccc caaaggcttt accgcgcagt ttccgcctta tcctttggac ggcgaattgt
    54661 atagcggacg tggtcagttc gagcagattt ccgctaccgt gcgttctgtt tcttcagact
    54721 ggcgcggcat ccgcctgcac gttttcgatg tacccaaggc gcagggcaac ctctaccaac
    54781 gtttggcagt cgcaacgcag tggctgaaaa cgcatccgaa cgcgccgatt accatcatcc
    54841 cgcaaatcaa agtgcgcgac cggcggcacg cgatggactt tttaaaacaa atcgaagcgc
    54901 agggcggcga aggcgtgatg ctgcgccagc ccgaatcccg ttacagcggc ggcaggagca
    54961 gccaattatt gaagctgaaa agccaatacg acgacgaatg cacggtaacg cggcactatg
    55021 agggcaaagg gcgaaacgcc ggacggctgg gcgcggtcgg ctgcaaaaac cgacacggcg
    55081 aattccgcat cggcagcggt ttcaaggaca aagaccgcga caacccgccc aaaatcggca
    55141 cgctgattac ctaccgttac cgtggcttta cgcggaaagg cacgccgaaa tttgccacat
    55201 ttgtgcgcgt gcgtaccgac cgctgagcag gacagttcag acggcatccg atactttggt
    55261 ttataatttt ccttttaccg accgattccg acatatgacc gatttagaaa ccaaacgcct
    55321 tgaaacacag gcgatgcttg aaaacgccga tcttttgttc gaccaaggcc aatgccgtgc
    55381 cgcactgcaa aaagtggcgg acgagattac gcgcgatttg ggggataaat atccgttgct
    55441 cctgcccgtg atgggcgggg cggtggtgtt tacggggcag ttgctgccgc tgttgcgctt
    55501 tcccttagac tttgattatg ttcacgtttc ccgttacggc gacaagctgg agggcggcgc
    55561 gttcaactgg aagcgtatgc ctgatgcgga acaaatccgg ggcaggcacg tggtcgtgtt
    55621 ggacgatatt ttggacgaag ggcatacgat gtccgccatt caagccaaac ttttggaaat
    55681 gggtgcggca agctgccgtg cggcggtgtt cgccaataaa ttaatcgaca aagaaaaacc
    55741 ggtcaaagcc gattatgtcg gactggatgt gccgaaccgt tatgttttcg gttacggcat
    55801 ggatgcggcg ggctgctggc gtaatttggg cgagatttac gcattgggcg gaaaataagg
    55861 gatgcgatgc cgtctgaagg cctttcagac ggcatcgcgc ccatacgccg gcaggattaa
    55921 tgaggaacag gacgcatcaa tatgataggg cttttaatca tcacacacga aaccataggc
    55981 gaagcctacc gcaagctggc gcatcatttt tttccgggcg ggctgcctga aaacgtccgc
    56041 atactcggcg tgcagccgac ggaaggccaa gacgacatta tcaacaacgc catttccgcg
    56101 ctgcaagagt ttcctgaaaa ccacggtgta ttgattatga ccgacatctt cggcgcgacc
    56161 ccctgcaatg ccgcccgccg gctcgtgcgc gaaaacaaat cggcgatttt gaccgggctg
    56221 aacgcgccga tgatgattaa ggccgtccaa tattcgccgg cggcggaaga ctttgccgcc
    56281 tttaccgaat gcgtcaggga ggcggcggta aaaggcattt tcgccatcac gtccgcgccc
    56341 gaagatttgg tgtgccggcg cagcggcgat gccgtctgaa gaagcggcag ggcaggaaaa
    56401 cattttaaac ggcgtctgct gccgatatat agcggattaa caaaaatcag gacaaggcgg
    56461 cgggccgcag acagtacaaa tggtacggaa ccggttcgcc tggtgcttca gcaccttagg
    56521 gaatcgttcc ctttgagcca aggcggggca acgccgtact ggtttttgtt aatccactat
    56581 acataacacg ggaaccgaaa tgctcaaaca atccatcgaa atcatcaaca aactcggact
    56641 ccacgcccgc gcgtccagca agttcaccca aaccgcgtcc caattcaaaa gcgaagtctg
    56701 ggttacgaaa aacggcagcc gcgtcaacgg caaaagcatt atggggctga tgatgctcgc
    56761 agccgccaag ggtacggtca tcgaactgga aaccgacggc ttggacgaag ccgcagccat
    56821 gaaggcattg accgacttaa tcaacgacta cttcggcgag ggcgaataat gagtatcgtg
    56881 ctgcacggcg tggcggcggg caaaggcatt gccgtcggtt gcgcccacct gattgcgcgc
    56941 ggtacggagg aagtgccgca gtatgatgtt gctcaggcgg acaccgatgc cgaagccgaa
    57001 cgtttcgatg ccgccgtcaa agccacgcgc aaagagttgg aacagctccg cagcgcgatt
    57061 cccgaaaacg ccccgaccga gttgggcgcg ttcatctcgc tgcacctgat gctcttgacc
    57121 gatgttacct tgtcgcgcga acccgtcgat attttaaggg aacaaaaaat caacgccgag
    57181 tgggcattga agcagcagag cgacaaactc gccgcccaat tcgacaatat ggacgatgcc
    57241 tatttgcgcg aacgcaagca ggatatgctg caagtcgtcc gccgcatcca caacaacctg
    57301 atcgggcagg gcaacgagtt ggaggttgcc gacaacctgt ttgacgaaac cgttctgatt
    57361 gcaaacgacc tttcacccgc cgacacggtt ttgtttaaag agcagcgcat tgccgccttc
    57421 gttaccgatg ccggcggccc caccgggcat acggcgattt tgggcaggag cttggacatc
    57481 ccgtccgtcg tcgggctgca caacgcgcgc aaactgatta ccgaaggcga aacggtcatc
    57541 gtggacggca tcaacggcgt gttgattatt tcgccggatg agtcggtgtt gaacgaatac
    57601 cgccgccgcg cccgcgaata ccgcagccac aaacgcgatt tgaacaagct caaaaaaacc
    57661 gccgccgcta ccgccgacgg ggtctgcatc gagcttgtgg gcaatataga atccgccgaa
    57721 gacgtgaaac cgctgcacaa cctcggcgca gacggcatcg ggctgttccg cagcgagttt
    57781 ctttacctga accgcgatac gatgccgtct gaagacgagc agtacgaagt gtacagcgcg
    57841 attgtcaaaa aaatgaaggg caaaagcgta acgatacgga cagtcgattt gggtgtggac
    57901 aaaaatccgc gctggttcgg gaaaaacagc acgcccaacg gcagcctcaa ccccgcgctg
    57961 ggcatgaccg gcatccgcct gtgccttgcc gaaccggtca tgttccgcac gcagatgcgc
    58021 gccatcctcc gtgcggcggt acacggtccc gtccgcatga tgtggccgat gattacctcc
    58081 gtatccgaag tgcgccagtg cctcatccac ctcgacaccg cgcaacgcca gcttgccgaa
    58141 cgcggcgatg ccttcggcga agtcggcatc ggctgcatga ttgaaattcc gtctgccgcg
    58201 ctgaccgtcg gcagtatttt gaaactggtc gatttcatct ccgtcggtac caacgatttg
    58261 atccaatata ttctgtccgt cgatcgcggc gacgacagcg tcagccatct ctaccaaccc
    58321 ggacatcctt ccgtgctgaa aatgctgcaa cacgtcatcc gtaccgccaa ccgcatggac
    58381 aaagacgtgt ccgtatgcgg cgagatggcg ggcgataccg cgtttacccg cgttttattg
    58441 ggtatggggc tgcgccgttt ttccatgaat ccgaacaaca tcctgcccgt caaaaacatc
    58501 attctgcaca gcaatgccgg acagctcgaa ggcgatattg cgaaagtcat ccgctgcgaa
    58561 gacgaagaga agtcggaaaa gctgatcaaa cagatcaaca gcgtgtctgt cgcggaagaa
    58621 gccgaagtca agggacggaa ataaatattg ttgacaatgc gagcgggttg cagtcaaata
    58681 acgcccttaa aaaatacagc tgctttggtc aagaaatgcc gtctgaaaaa ttttcagacg
    58741 gcattttgtt tggcaggcat ttctgccgca tcatcggaaa cggacgatac gcccttcatc
    58801 gacgcggatg cggaccgtac cgtttccgga aataccgtcc gtatgttgtc cgacagtcag
    58861 gtttaaggtc agctcgccgt gttcgggatg gacggcggaa agccggagcg agtcgggcag
    58921 gcggacgagg gacagcaggc ggcattccgt tccatgattg tccaagcaca cggcattttg
    58981 cggaatatgg cggtcatcgt cggtattggg cagccccatc agacgggcga cctgcacgcc
    59041 ggcaggcgtt tgaatcaagg tttcgggcgt accgcattga aggattttcc cctcgtgcat
    59101 gacggcgatt tcgtccgccg ccgtgcaggc ctcttcgggc gaatgcgtta ccaaaacggc
    59161 agggatgccg cccttgcgga tgcgttcggc ggtcatacgg cgcagccggt cgcgcaaatg
    59221 cgtgtccaaa ctggaaaacg attcatccag caacagcagg gaagggcgga caaccaaagc
    59281 gcgcgccagt gccaaccgtt gcttctcgcc tccggaaagt ttttcaggct tgcggtgcgc
    59341 ctcgttttcc agcccgactt cggcaagtgc cgacaaggcg aggcgttcgg cttcggcttt
    59401 cggcattttt tgcattttca aaccgaatgc cgtattttcc agcgcactca tatggggaaa
    59461 cagcgcgtaa tcttgaaaca tcagcgagat acggcgtttt tcgggcggca tacaggtaat
    59521 gttttccccg ttcagccgaa tttcgccgcc gtccggccgg acgatgcccg caatcatatt
    59581 cagcagggtg gatttgccgc agcccgaccg ccccagtacg gcgagtattt tgccgcgccc
    59641 gacagtcagg cagatgttgt cggcgaccgt tttgccgccg aagcatttgc agagtccgtt
    59701 cagttcaagc atggcgcatc ctataaacgt atgccgtcca gccactcgga cagcggatgg
    59761 gcggcatcaa tcagaccgta acggtaaaac gcgtccagcg taaccagttg cgcgtcgtgc
    59821 atcatgtttt tcgacaacat ggcatccaat aggccgccaa tgtccatctt ttcaaaaccc
    59881 gctacctcgc catcctgatt ttcaggcagg aaggtttcgg gcaggacggc atcgaacaca
    59941 tacaggattt cattgtgcac acctcggctg acggggcgaa ggctgtgcag ccgcgatact
    60001 gggcggatga gcggaaacag cgttttatcc aaaccggctt cttcgctgct ttcgcggcac
    60061 acggcttcag acggcatttc gccgccggaa acaccgccgc cggcaatatt gtcgagcttg
    60121 ccgggatcga ctgctttgtg cggactgcgc ctgcctatcc aaaaatgcca tctgccgttc
    60181 gattcgacca aaccgttgag atggacggcg cggctgagta gtccgaacgg acggaaagcg
    60241 gcgcgttcga gcgtgaacaa ggggttgccg ccgccgtcgg tcaggtcgaa acactcgttg
    60301 cgccatccgt gaagcagccc cgccttgttc catgtgcggg cgaggtgctg caagcgtccg
    60361 cccatatccg gccagccgtc cgcattcaga aaaatgccgt ctgaagactc ggagcagcct
    60421 gcctcccagt cttttttgat gcgctcagcc cattccggcg acagattgcc caaaggaaga
    60481 cggttcagat acagcgtttt ccagcaactt tcggcaccgt aacttgcttt tgcccgctcg
    60541 aacagggcat caaggtcttg tttgctgacg gattcggtaa aacggacggt cggcatagcc
    60601 ggctttcttg aacacaatgc ggaagattgt agccaaaccg cctgaatttg caaccgcaaa
    60661 cagacggcgg cctgccgttc cgatattgcg gcaggaagga aaatccggca aaaaacggag
    60721 gcggcaggaa acaggaaaaa caaaaccccc tcggcattga accgaggggt ttgatatttg
    60781 gttgcgggag caggattcga acctacgacc ttcgggttat gagcccgacg agctaccatg
    60841 ctgctccatc ccgcgtcaga agatgaaact atacggcaga ttttttatgt tgtcaaacat
    60901 tatttccgtg aaaaatcata aagttttccg tccggcggct tatgcctgat ttgaaatatc
    60961 attttcttta caaaagttca tgttcgtgat ttaattttgg ttaacatcga aacaggggtg
    61021 ctgcctgatg ttcaggcggc tgagaaatac cctttacacc cgatcgggat aatacctgcg
    61081 tggggagttt tcacggattc tgtctttcag acggcattgg ttttcaaatg ccgtctgaaa
    61141 acgcaaaacg ctcctgtttc tttattctaa acgagaaaac aggagcattt tttatgacta
    61201 cgccaaaaaa aaccgccaaa acttccggca acgaagcgcg cgaactttcc gacttgagcg
    61261 aagacatcgg catccgcttc aaatatcaga actccgaacg cgtgtatctg caaggcagcc
    61321 gcgacgacat ccgcgtgcct ttgcgcgaaa tccgtcagga cgacacctac acggcgcaag
    61381 gtacggaagc caacccgcct attccggtgt acgacaccag cggcgcatac ggcgatccgg
    61441 cggcgcacat cgacctgaaa caaggcctgc cgcacatccg cacggcgtgg ctggacgaac
    61501 gcggcgatac cgaaatcctg cccaagctct ccagcgaata cggcatcgaa cgtgcgcacg
    61561 atccgaaaac cgcccatctg cgcttcaacc aaatcacccg cccgcgccgt gccaaagccg
    61621 gccgcaatgt aacccagctc cattacgccc gccaaggcat tatcacgccc gaaatggagt
    61681 tcgccgccat acgcgaacgc atgaagctgg acgagctttt cagacggcct gaatacgcca
    61741 agctcttgaa acagcacaca gggcaaagtt tcggtgcgaa catcccgacc cgtcccgacc
    61801 aaatcacgcc cgaattcgtg cgccaagaaa tcgccgccgg acgcgcgatt atccccgcca
    61861 acatcaacca ccccgaactc gaaccgatga ttatcggccg caacttccgc gtcaaaatca
    61921 acggcaactt gggcaattcc gccgtaacct ccagcctgac cgaagaagtc gaaaaaatgg
    61981 tgtggtcgct gcgttggggc gcggacacga ttatggactt atccacaggc gcgcacatcc
    62041 acgaaacgcg cgaatggatt atccgcaacg cgcccgtccc catcggcaca gtgccgattt
    62101 accaaacttt ggaaaaaacc ggcggcatcg ccgaagattt gacttgggat ttgttccgcg
    62161 acaccttaat cgagcaggcg gaacaaggcg tggactattt caccatacac gcgggcgtgt
    62221 tgctgcgtta tgtgccgatg accgccaacc gcctcaccgg catcgtatcg cgcggcggtt
    62281 cgattatggc gaaatggtgt ttggcacatc atcgggaaaa cttcctctac acgcatttcg
    62341 acgaaatctg cgaaatcatg aaggcgtatg acgtatcgtt cagcttgggc gacggcctgc
    62401 gccccggctg cattgccgat gccaacgacg aatcccaatt cgccgaattg cacaccttgg
    62461 gcgaattgac cgataaagcg tggaaacacg acgtacaagt catgatcgaa ggccccggcc
    62521 atgtgccgct gcaacgcgtc aaagaaaata tgaccgaaga gttgcaacac tgctttgaag
    62581 caccgtttta cacactcggc ccgctcgtta ccgacatcgc ccccggctac gaccacatca
    62641 cttcgggcat aggcgcggcc aatatcggct ggtacggcac ggcgatgctt tgttacgtta
    62701 cccccaaaga acatttgggg ctgcccgaca aagaagacgt gcgcaccggc atcatcacct
    62761 acaaactcgc cgcccacgcc gccgacctcg ccaaaggctg gccgggcgcg caattacgcg
    62821 acaacgccct gagcaaggcg cgtttcgaat tccgctggcg cgaccaattc cgcttaagcc
    62881 tcgaccccga acgcgccgag agcttccacg acgaaaccct gcccgccgaa ggcgcgaaaa
    62941 tcgcccattt ttgctcaatg tgcggcccca aattttgctc gatgaaaatc acgcaggaag
    63001 tgcgcgacta cgccgacaag caaaaagccc aacggcaggg tatggaggaa aaagcggtcg
    63061 agttcgtcaa aaaaggcgcg aagatttaca gttgaaacgt caagcaaaaa atgccgtctg
    63121 aagcccggaa aaaaggcttc agacggcatt ttttcgcttg tgaaaatgat taaacaaccg
    63181 atcattaaag taatttttaa aatttttata ttcacataga tgggaatgat ggatttaggg
    63241 ttctgatttt gttttgagag aatgaaggaa tttgagattg tgggcgatta tcgggaaaaa
    63301 tagaatcttt c
//
LOCUS       DAXDXW010000002        48131 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXDXW010000002 DAXDXW010000000
VERSION     DAXDXW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 48131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48131
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.002"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(123..935)
                     /locus_tag="ACFB64_000072"
     CDS             complement(123..935)
                     /locus_tag="ACFB64_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC1208537.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTGKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGCDGVRTGLYHKMHLFGFSGLGERYAEADTIRAGREVPHLSAEGMPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKHRVLDAV"
     gene            1020..1892
                     /locus_tag="ACFB64_000073"
     CDS             1020..1892
                     /locus_tag="ACFB64_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC1208538.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVINRASVVIDADSIGGADLVLIATPVATVPAVLTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRNGAQAAQFGLFRHRKLIITPHGGEDSDGI
                     ALVENLWRAVGADIFTMDAQHHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLADILTADDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            complement(1950..3104)
                     /locus_tag="ACFB64_000074"
     CDS             complement(1950..3104)
                     /locus_tag="ACFB64_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC1208539.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTAFWLAARIAAFIPGWG
                     AAASGILGTLFFWYGAVCMALPVIRSQNRRNYVAVFAIFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGMRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAIL
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNSIYPPPKAVPVAFWLMMVAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(3149..3517)
                     /locus_tag="ACFB64_000075"
     CDS             complement(3149..3517)
                     /locus_tag="ACFB64_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC1208540.1"
                     /translation="MKPLKRHSALVGLSREHHHSLSLCVRMLRTPGADHRAELELHFA
                     ELETHFREEEAKFAPIWQNVAPELKTRFEGDHAKLRQMMASPECGNAAWNTAFATTLR
                     DHARFEERELFPAVEPFLPA"
     gene            3691..4134
                     /locus_tag="ACFB64_000076"
     CDS             3691..4134
                     /locus_tag="ACFB64_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC1208541.1"
                     /translation="MYLTQHTDYGLRVLIYTAVNDDALVNIATIASTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPERINIGAVVRHLEPMQLVECMGPNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYESKIPIAVR"
     gene            4144..4272
                     /locus_tag="ACFB64_000077"
     CDS             4144..4272
                     /locus_tag="ACFB64_000077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208542.1"
                     /translation="MPPKPAFGRHFVCRFKRIFRPGAIRAGISGCRRQGARCRLNG"
     gene            complement(4296..4916)
                     /locus_tag="ACFB64_000078"
     CDS             complement(4296..4916)
                     /locus_tag="ACFB64_000078"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC1208543.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIINQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKDAPARALHPKRKTIGLRIPDNAVAQALLEELGGPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTDPTTVIDMTDGTELVRRGFGDTAVFGL"
     gene            complement(5027..6226)
                     /locus_tag="ACFB64_000079"
     CDS             complement(5027..6226)
                     /locus_tag="ACFB64_000079"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC1208544.1"
                     /translation="MSDQLILVLNCVSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYHESVL
                     IDQDVLDELKACIPFVPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTGMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAVKN
                     GKSVDTGMGFTPIEGLVMGTRCGDTDPGVYSYPTFHAGMDVAQVDEMLNKKSGFPGIS
                     ELPNDCRTLEIAADEGREGARLALEVMTCRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            complement(6538..7700)
                     /gene="prpF"
                     /locus_tag="ACFB64_000080"
                     /pseudo
     CDS             complement(6538..7700)
                     /gene="prpF"
                     /locus_tag="ACFB64_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
     gene            complement(7816..10421)
                     /gene="acnD"
                     /locus_tag="ACFB64_000081"
                     /pseudo
     CDS             complement(7816..10421)
                     /gene="acnD"
                     /locus_tag="ACFB64_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
     gene            complement(10498..11286)
                     /locus_tag="ACFB64_000082"
     CDS             complement(10498..11286)
                     /locus_tag="ACFB64_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFC1208545.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKSLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIVATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            complement(11438..12586)
                     /gene="prpC"
                     /locus_tag="ACFB64_000083"
     CDS             complement(11438..12586)
                     /gene="prpC"
                     /locus_tag="ACFB64_000083"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC1208546.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSHRGYD
                     ILNLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLETLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPDSEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETVGGHFLHLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSC
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEADIRERIGRKEIVIGFGHPVYTISDP
                     RNVVIKEVARGLSREAGDMRLFDIAECLESVMWEEKKMFPNLDWFSAVSYQKLGVPTA
                     MFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(12671..13546)
                     /gene="prpB"
                     /locus_tag="ACFB64_000084"
     CDS             complement(12671..13546)
                     /gene="prpB"
                     /locus_tag="ACFB64_000084"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFC1208547.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVVGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLMVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLKMYRQFADAVKVPVLANITEFGATPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFDQKLDKLFQK"
     gene            complement(14166..15476)
                     /locus_tag="ACFB64_000085"
     CDS             complement(14166..15476)
                     /locus_tag="ACFB64_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC1208548.1"
                     /translation="MDISKQTLLDRVFNLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFIGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFVMVVVLGYFRVQGAIIITILTITVIASLMGLN
                     EFHGVVGEVPGIAPTFMQMDFKGLFTVSMVSVIFVFFLVDLFDSTGTLVGVSHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPVFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTGDVPPMVWVVAVLWALKFWYLG"
     gene            complement(15612..16790)
                     /gene="ftsZ"
                     /locus_tag="ACFB64_000086"
     CDS             complement(15612..16790)
                     /gene="ftsZ"
                     /locus_tag="ACFB64_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC1208549.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRSVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFSYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDPTPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(16909..18153)
                     /gene="ftsA"
                     /locus_tag="ACFB64_000087"
     CDS             complement(16909..18153)
                     /gene="ftsA"
                     /locus_tag="ACFB64_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC1208550.1"
                     /translation="MEQQKRYISVLDIGTSKVLALIGEVQDDDKINIIGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIELCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGT
                     VQEREGGGGLLARLKRWIENNL"
     gene            complement(18239..18967)
                     /locus_tag="ACFB64_000088"
     CDS             complement(18239..18967)
                     /locus_tag="ACFB64_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC1208551.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWNVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRHAPDGLPEKESEE"
     gene            complement(18957..19871)
                     /locus_tag="ACFB64_000089"
     CDS             complement(18957..19871)
                     /locus_tag="ACFB64_000089"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC1208552.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKERGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLYDDTDFDAVEEKLGLPMFVKPAVEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVTGVGFADLCIEILKAAHVG"
     gene            complement(19983..21392)
                     /gene="murC"
                     /locus_tag="ACFB64_000090"
     CDS             complement(19983..21392)
                     /gene="murC"
                     /locus_tag="ACFB64_000090"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC1208553.1"
                     /translation="MMKNRVSNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLSSLGIQVYPGHTAEHVNGADVVVASTAVKKENPEVVAALERQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCV
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPVAAADSRALARAIRVLGKLEPIYCENVADLPQMLMNVLQDGDVVLNM
                     GAGSINRVPSALLELSKQI"
     gene            complement(21547..22614)
                     /gene="murG"
                     /locus_tag="ACFB64_000091"
     CDS             complement(21547..22614)
                     /gene="murG"
                     /locus_tag="ACFB64_000091"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC1208554.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRVRGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGIRGNGIKRKLMLPFTLYKTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPEEVRPQMYHQ
                     SGRNKLGNLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(22618..23889)
                     /locus_tag="ACFB64_000092"
     CDS             complement(22618..23889)
                     /locus_tag="ACFB64_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC1208555.1"
                     /translation="MGDGVHTLLLDRPIVRDGRKFDAPLLWMVVLMTAFGLLMIYSAS
                     VYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRTWRRLVPWIFALSGLLLVA
                     VLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLFTRREEVLRSMESLGWQSI
                     WRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFGLVLIMVQPDFGSFVVITV
                     ITVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRVVAFLDPWKDPQGAGYQL
                     THSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFGFFGMCVLIFCYGWL
                     VVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGVNIGALPTKGLTLPLMSYG
                     GSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(24068..25405)
                     /gene="murD"
                     /locus_tag="ACFB64_000093"
     CDS             complement(24068..25405)
                     /gene="murD"
                     /locus_tag="ACFB64_000093"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC1208556.1"
                     /translation="MTFQNKKTLVAGLGGTGISMIAYLRKNGAEVAAYDAELKAERVA
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAELQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVMNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDVFCRAMKRAGREVKRFSLEHEADFWLERGTGCLKQGNEDLISTQDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALKDKAKGVFLIGVDAPQIRRDLDGCGLN
                     LTDCVTLEEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(25490..25870)
                     /locus_tag="ACFB64_000094"
     CDS             complement(25490..25870)
                     /locus_tag="ACFB64_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC1208557.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWAGDVSDFRENLQAAEQGNAAAQ
                     FNLGVMYENGQGVRQDYVQAVQWYRKASEQGDAQAQYNLGLMYYDGRGVRQDLALAQQ
                     WLGKACQNGDQNSCDNDQRLKAGY"
     gene            complement(25995..27077)
                     /gene="mraY"
                     /locus_tag="ACFB64_000095"
     CDS             complement(25995..27077)
                     /gene="mraY"
                     /locus_tag="ACFB64_000095"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC1208558.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAVIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLAAFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(27322..27498)
                     /locus_tag="ACFB64_000096"
     CDS             complement(27322..27498)
                     /locus_tag="ACFB64_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208559.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVPAR"
     gene            complement(27501..28859)
                     /gene="murF"
                     /locus_tag="ACFB64_000097"
     CDS             complement(27501..28859)
                     /gene="murF"
                     /locus_tag="ACFB64_000097"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC1208560.1"
                     /translation="MKPLDLNFICQALKLPMPSENKPVSRIVTDSRDIREGDVFFALA
                     GGRFDAHDFVGGVLSAGAAAVVVSREDCAALGGALKVDDTLAALQTLAKAWRDNVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVSATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNALRAHVGCGFDGVGDIAKAKSEIYAGLCSDG
                     MALIPQEDANMAVFKTATFNLNTCTFGVDSGDVRAENIVLKPLSCEFDLVCGDERTAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLQGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLSHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(28984..30630)
                     /locus_tag="ACFB64_000098"
     CDS             complement(28984..30630)
                     /locus_tag="ACFB64_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC1208561.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSVGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMEKIAGDKDWREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSRTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLTGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGEWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVPGEGNNLAFIDRQSDDPAPWYAGKGKSAKNTSKK"
     gene            complement(30653..32131)
                     /locus_tag="ACFB64_000099"
     CDS             complement(30653..32131)
                     /locus_tag="ACFB64_000099"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC1208562.1"
                     /translation="MFSKLTPLAETNIPPLLCANAAGRLLHSDSRQIKQGDIFVACQG
                     EYTDGRSYIPAAVVNGAAFVFWDDDGKFAWNPEWKVPNQGIKNLKHRAGMLAAQVYDN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIIGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAIINVDDEYGAELIGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGMEAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALSTLQEIKPQGAALWCVFGCGGNRDCG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPQDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(32156..33907)
                     /locus_tag="ACFB64_000100"
     CDS             complement(32156..33907)
                     /locus_tag="ACFB64_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC1208563.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAMAVLFACLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQALPATRGTVSDRNGAVLALSAPTESLFAVPKDM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLYGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPQNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVR
                     MHSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPLSF
                     EKQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTVRKLVNG
                     RYVDNKHVGTFIGFAPAKNPRVIVAVTIDEPTANGYYGGVVAGPVFKQVMGGSLNILG
                     VSPTKPLTNVAAVKTPS"
     gene            complement(33968..34231)
                     /gene="ftsL"
                     /locus_tag="ACFB64_000101"
     CDS             complement(33968..34231)
                     /gene="ftsL"
                     /locus_tag="ACFB64_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC1208564.1"
                     /translation="MNKSNFFLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRVQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(34258..35214)
                     /gene="rsmH"
                     /locus_tag="ACFB64_000102"
     CDS             complement(34258..35214)
                     /gene="rsmH"
                     /locus_tag="ACFB64_000102"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC1208565.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIVRAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHEPLPSWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPE"
     gene            complement(35211..35666)
                     /gene="mraZ"
                     /locus_tag="ACFB64_000103"
     CDS             complement(35211..35666)
                     /gene="mraZ"
                     /locus_tag="ACFB64_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC1208566.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVATLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            35945..37192
                     /locus_tag="ACFB64_000104"
     CDS             35945..37192
                     /locus_tag="ACFB64_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC1208567.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTFIMLLPSPEARQSSLNLQTLIAE
                     EIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTPLFAQTLARQL
                     QELLPQTAGNIYEFGAGTGQLAADLLGSVSDSINCYYIIEISPELAARQKNLIQARAP
                     EASQKVVHLTALPEAFDGIIIGNELLDAIPVEIVRKNEGGLLEHIGVCTDNGRFAYSA
                     RPLHDPSLSTSASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQ
                     YYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYLPQ
                     SHFLLNLGITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGIDWA
                     GFRFGDICHKLQPSCRLNPLQTA"
     gene            37288..37364
                     /locus_tag="ACFB64_000105"
     tRNA            37288..37364
                     /locus_tag="ACFB64_000105"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37322..37324,aa:Met,seq:cat)
     gene            37372..37447
                     /locus_tag="ACFB64_000106"
     tRNA            37372..37447
                     /locus_tag="ACFB64_000106"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37405..37407,aa:Ala,seq:ggc)
     gene            complement(37633..38454)
                     /locus_tag="ACFB64_000107"
     CDS             complement(37633..38454)
                     /locus_tag="ACFB64_000107"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC1208568.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFDK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTVMGGMFWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(38487..39131)
                     /locus_tag="ACFB64_000108"
     CDS             complement(38487..39131)
                     /locus_tag="ACFB64_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC1208569.1"
                     /translation="MKFKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQPGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDTYLRTEHVVWRPEMLGLARMAAAVKLSGL
                     KYQANSAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAVALKMQK
                     LTEQYGIDGTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(39134..39997)
                     /gene="ftsN"
                     /locus_tag="ACFB64_000109"
     CDS             complement(39134..39997)
                     /gene="ftsN"
                     /locus_tag="ACFB64_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC1208570.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILLYLNQGGQNAFKIPA
                     PSKQPAETEILKLKNQPKEDIQPEPADQNALSEPDVAKEAEQSDAEKAADKQPVADKA
                     DEVEEKAGEPEREEPDGQAVRKKALTEEREQTVREKARKKDAETVKKQAVKPSKETEK
                     KASKEEKKAAKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTFGKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSEVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHG
                     VASLIRAIEGK"
     gene            complement(40103..41599)
                     /locus_tag="ACFB64_000110"
     CDS             complement(40103..41599)
                     /locus_tag="ACFB64_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC1208571.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHSARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLAEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARGKQYARQGKVNAALSVSELDSQACIQKEAQEAL
                     GSLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(41613..41933)
                     /locus_tag="ACFB64_000111"
     CDS             complement(41613..41933)
                     /locus_tag="ACFB64_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC1208572.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            42421..43947
                     /gene="putP"
                     /locus_tag="ACFB64_000112"
     CDS             42421..43947
                     /gene="putP"
                     /locus_tag="ACFB64_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC1208573.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGASTVVVWAEWV
                     KKPARAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSREIQERFEKADADYRAAR
                     "
     gene            44199..47804
                     /gene="putA"
                     /locus_tag="ACFB64_000113"
     CDS             44199..47804
                     /gene="putA"
                     /locus_tag="ACFB64_000113"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC1208574.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALGRLIGKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDRLIKSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHGDVVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLILGAGD
                     TGAALTNDASIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKAAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADKAALKRL
                     EALIHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTGLAASGVQAVVEPDSPLASYTADLDGLLQISGKPENTGVNHVAALSP
                     LDSSRKQELANRDGALIRILPSGQGLDILQVFEEISCSINTTAAGGNAGLMAVAD"
BASE COUNT        11187 a        13681 c        12221 g        11042 t
ORIGIN      
        1 gacgcggcgg gaatctatcg gaaatgactg aaaccccgag attctagatt cccacttccg
       61 tgggaatgac ggttcggttg ctacggcccg ctgattcccc gacaccgatg ccgtctgaac
      121 tttcagacgg catccaaaac ccggtgtttc aaggcgggca ggcggttgcg gacgctgttc
      181 aggcggttgg catcgatgtc tgccgtaacg atgccttcgc cttcgggtaa tacgtccaac
      241 acgtcgcccc acggatcgac aatcatactg tgtccgaacg tgcgccgtcc gttttcgtgc
      301 aaaccgccct gtgccgccgc cacgacgtaa cattggtttt cgacggcacg cgcgcgcagc
      361 agcagctccc agtgcgcctt gcccgtcgta tgggtaaatg ccgccggcag catcaatacg
      421 tcaaacggca gttggcggcg gaaaaattcg ggaaagcgga catcgtaaca aatacctgcc
      481 gccaccggca tgccttctgc cgacaagtgc ggcacttccc gccctgcgcg gatggtatcg
      541 gcttcggcat agcgttcgcc caaaccggaa aaaccgaaga ggtgcatttt gtggtacagc
      601 cccgtcctta cgccgtcgca cccgtacacc aataaagtat tcatgacctt gcccgcttcg
      661 gggctttgca gcggcacggt tccgccgaac agcaccacgc cgcattcttt cgccgtttcg
      721 ctcaatgccg tctgaaagcg tccgccgccc aaaggctcgg caagtgcgag tttgccggta
      781 tcgtttgcgc ccatcagcac ccaatattcg ggcagcagca cccaatccgc accctgctcc
      841 gccgcccgtg cgaccaggcg tttcatggcg gcgacgttgg tttccggcga cacgcccgac
      901 accatctgca cggcggcaac tctgattttg tccatttctt ctcctttttc ctgccgacct
      961 tgcggcatat tccgccgata ggctaccatg gcggcacttt gcgaaaaccg tttacacaaa
     1021 tgcccatttt gaaccatatt gccctcatcg gtgtagggct gatcggcggt tcgttcgttc
     1081 tcgacctcaa aaggcaggga ctcgtccgca ccgttaccgg tatcgacacc gaccgcgaca
     1141 acctcgaacg tgcattggaa cgcggcgtga ttaaccgggc ttccgtcgtc atcgacgcgg
     1201 acagcatcgg cggtgcggac ttggtactga ttgccacgcc cgtcgccacc gttcccgccg
     1261 ttttaaccgc gctgcgcccc gttttgccgg aacacacttg gatttccgat gtcggcagca
     1321 ccaaatcttc ggtcatcgaa gccttccgcc gctgcctgcc cgaccgcctg caccgctgca
     1381 tcgccgcaca cccgattgcc ggttcggaca gaaacggtgc gcaagccgcg cagttcgggc
     1441 tgttccgcca cagaaaactc atcatcacgc cccacggcgg cgaagattca gacggcatcg
     1501 cattggtaga aaacctgtgg cgcgcggtcg gtgcggacat tttcacgatg gacgcgcaac
     1561 accacgacgc ggttttcgcc gccgtctccc atatgcccca cctgaccgcc ttcgcctatg
     1621 tccaccagat tctcgaccac cccgacgggc aggaatatct gaaattcgcc gccacgggct
     1681 ttcgggactt cacccgcatc gcctccggcc atcccgccgt gtgggcggac atctgccttg
     1741 ccaacaaaga cagcctgctg caactggttc aaggcttggg caaacagttg gacgttttgg
     1801 cagacatcct gaccgccgac gaccgcgaag ccctgtaccg ctattttgaa gaagccaaaa
     1861 ccacgcgcga ccgctggctg gacggcaact gaccgccatg ccgtctgaaa agcaaaccgc
     1921 cgcagacccg aaatctgcgg cggttttact caaccgggcc tgccgtccga acgcggacgg
     1981 atcagccacg gaatgtattt ccacgcatac accagcagcg cgagtgcaaa caaaaccgaa
     2041 gacgtgcgga tgctgtgcgt gtaggcagtg ccggaagaaa atacggcaac catacggacg
     2101 gcggttgcca ccatcatcag ccaaaacgca acgggaacgg ctttgggcgg cggataaatc
     2161 gaattgcccg tatgaccgag cgcggtacgc gccatcatgc ccaaagtcag cacgccgata
     2221 ccgccgaccc cgatcagatg tacgcccaga ttgaggaagg caggtttgaa ataagacgcg
     2281 ccgaccgcaa tcagccccaa tccggtaaac agatagccgg caaacagaat ccacagcatc
     2341 ggttctttca atacgggttt ataccaccag cggtacacct gtacggtaaa aatcacgccc
     2401 gccgcaaacg cgaaagccgc cgacagccaa ggcatcacgc cgtgcgccat cagtatggcg
     2461 gtcagcatgg gtagccacag cgaagcctgc gccacccatt tcggactggg aatctgcggc
     2521 acgttcaacc gtttggacgt aaaaaacgaa ataatcctca tcccaatcag gccgataaag
     2581 cccgacacca taaccaggcc cgactgcaat ccgctcaaga gtccgcctag gttgccgttg
     2641 tgcagctgga cgtggaacgc cgcatgcgta ccgcccagca caaatattgc gaatacggcg
     2701 acatagttgc gccggttttg cgaacggata acgggcaaag ccatgcacac cgcgccgtac
     2761 cagaaaaaca gcgtaccgag tatgccgctt gccgccgcac cccaacccgg gataaaggcg
     2821 gcaatccgcg cagccagcca aaaggcggtc aagccgacca gaacgccgcc cctcgtgggc
     2881 ggctgtcccg tccaagtggc gacggcggtc agcaggaagg cgatgacgac gagaccggca
     2941 taaccccaaa tcatctcatg cgcgtgccaa tagaaaccgg acagctcgtg cgttcccgtg
     3001 tagccgaaac cccacagcaa tacggacaat gcgccgtaca gtgccgccag tgaataaaac
     3061 gggcggaacg ccattgccca gacgggatgt ttggtaaatt tcatgtgtgt tccttgttta
     3121 tcattaatat atttgccgca aaacggaatc atgccggcaa aaacggttcg acggcgggaa
     3181 acagctcgcg ttcttcaaag cgcgcgtggt cgcgcagggt tgtggcaaaa gcggtattcc
     3241 acgccgcgtt accgcattcg gggcttgcca tcatctgccg cagtttggcg tgatcgcctt
     3301 caaaacgggt tttcaactca ggcgcgacat tctgccaaat tggggcaaac ttggcttctt
     3361 cttcgcgaaa atgggtttcc agttcggcaa aatgcagctc gagttcggca cgatggtctg
     3421 ccccgggcgt ccgcaacata cgcacgcaca gggaaagcga gtggtggtgt tcgcgcgaaa
     3481 gcccgacaag ggcggaatgt cgtttcaacg gtttcataaa tttaggttac aataacaaat
     3541 tatcaaacgg tagcgcggat gtttcagacg gcataccgcc gcagacaggc tacattctac
     3601 cgccccgacg gacagggttt caaccattaa ggtattttcc gtgaaactta aaccgtaccg
     3661 ataagaataa cgatccatac cgagaaaatc atgtatctga cccaacatac cgactacggg
     3721 ctgcgcgtcc tcatctacac cgccgtcaac gacgacgcgc tggtcaatat cgccaccatc
     3781 gcctcgacct acggcatttc caaaagccat ttgatgaaag tggttaccgc gctggtcaaa
     3841 ggcgggttct tgcacagcgt acgcgggaaa ggcggcgggc tgaggctggc cgcgccgccc
     3901 gaacgcatca atatcggcgc ggtcgtccgc cacctcgaac cgatgcagct ggtcgagtgt
     3961 atgggcccga acaacgaatg cctgattacg ccgtcctgcc ggctgacggg catactcggc
     4021 ggtgcaatga agtcgttttt cacgtatctg gacggtttca cgctgcaaga cctgctcaac
     4081 aagccgacct acgacctgct ttatgaatcg aaaatcccga ttgcggtgcg ataacgcatc
     4141 aaaatgccgc ccaaacccgc gttcggacgg cattttgttt gccgtttcaa acggatattc
     4201 cgaccgggcg cgatacgcgc cggcatttcg ggctgccgcc ggcaaggcgc gcgatgccgt
     4261 ctgaacggct gatgcttcag acggcatcgg tttccctaca aaccgaacac cgccgtatcg
     4321 ccgaaacccc ggcgcaccag ttccgtgccg tcggtcatgt cgatgacggt agtcggatcg
     4381 gttccgcacc agccgccgtc aattaccaaa tcgacggcgt gttccaaacg ctcgcggatt
     4441 tcataagggt cggtcaatgg ttcgccgtct tcgggcagca tcagggtgca gcttaaaagc
     4501 ggcccgccca actcctccag cagggcttgt gcaacggcat tatcgggaat acgcagcccg
     4561 atggttttgc gtttcgggtg cagggcgcgc gccggcgcat ccttcgtcgc ctgcaagata
     4621 aaggtataag gcccgggcgt ggcggcttta agctgacgaa actgtacgtt gtcgattttg
     4681 gcgtatgtgc ccaactcgct caaatcggcg cacatcaggg tcaggtggtg tttcaaatcg
     4741 atttttcgga tggagagtat gcgttccatc gccgccttat cgccgagttt gcaacccaag
     4801 gcgtaacagg aatcggtcgg ataaaccacc acgccgcctt gattgatgat ttcgactgcc
     4861 tgcttgatga ggcgttcttg gggattgtcg ggatgaacgg cgaaaaattg tgccatagtt
     4921 tgtttcctta tcgtcatgtt ttccgtattg tacgcccaaa cgccaaacaa aatgccgtct
     4981 gaaaaccttt cagacggcat ttttgcggac tcgccccctt ttttggctac aagatgccgg
     5041 caagttcggc agtgtcgcag gcaatcatca gttcttcatt ggtcgggacg accaaaacag
     5101 ccggagaaga atcggtcggg ctgataatgc ccgaattgcc gtagcgtttt tccatattgg
     5161 ctttggtgtc gatgtgcaaa cccaagaaat caagatagga aacggttttg gcacggatat
     5221 tacgcgagtt ttcgccgata ccgccggtga acacgagtgc gtcaacaccg ccgcaggcca
     5281 cagccatcga agcgatgtat ttggcgaggc ggcaggtcat gacttcgagg gcgaggcgcg
     5341 cgccttcgcg gccttcgtcg gcggcgattt cgagggtgcg gcagtcgttg ggaagttcgg
     5401 aaataccggg gaaacctgat tttttgttca gcatttcatc aacttgggca acatccatcc
     5461 ctgcgtggaa agtcggatag ctgtatacgc ccggatcggt gtcgccgcaa cgtgtaccca
     5521 ttaccaaacc ttcgatcggc gtgaaaccca taccggtatc gacggatttg ccgtttttga
     5581 cggcggtaat ggatgcgccg ttgcctaagt gggcaataat catgcggatg tcttccagag
     5641 gtttgcccaa gatgcgtgcg gcttcagggg cgacgtaacg cataccggta ccgtggaaac
     5701 cgtagcggcg gaaggcgtat tttttgcgca attcgcgcgg cacggcataa gtgtaggccc
     5761 gctccggcat ggtttggtgg aacgaggtgt ccatcacgcc gacgttgggc aggccgggaa
     5821 agtgttcctg cgcggcgagg atgccgctga tgttggcggg gttgtgcagc gggacgaacg
     5881 ggatgcaggc tttcagttcg tcaaggacgt cttggtcgat gaggacggac tcgtgatatt
     5941 tttcgccgcc gtgggcgatg cggtggccga tggctttgat gcggtcgtgc agtccgtgtt
     6001 tttccagttc gttcaacagc atacccaccg cgccggcgtg gcagttgcgg ccgctcaggg
     6061 gaacttggcg tttgttgccg tctttgttga aggtaatgac ggcttcgggc gtagtcaggc
     6121 gttccccgag gcagcttagg acgacgctgc cgcttttgcg gtcgataacg gcgcctttga
     6181 gcgatgaact gacgcagttc aggacaagaa tgagttggtc ggacatgatg tttctccttg
     6241 tagtttgtgg ttgttttttt ggtgcggggc cgtctgaaaa aggatgggcg ttgggcggag
     6301 tgtgtttcag acggcctctt tgggcaaagc gtatgccgta tttcctagcg tcattcccac
     6361 gcaggcggga atccatattt gctcacacgc aggctctgat aaaaatcagg aaacagaata
     6421 tcggctgcgg attcccgcct gcgtgggaat gacggcggta aggttattcg gctttatggt
     6481 aatccttaat caaatgccgt ctgaaacagc ggacagcaat gtgcgtccta catgatttca
     6541 aaaacaatca tcgggaacgc gcacccaacc ttccataatc acgcgtgcgc tgcggctcat
     6601 gaccgctttg gcggccgtcc attgtccgtc ctgacattcg gcggcagcac cgacacgcag
     6661 cgtacctgac ggatgcccga agcgcacttc tttacgcgtt ccgccgcctg cggcaaggtt
     6721 gaccagcgta ccgagcacgg cggcggcgat ggcgaccgag gcgatgccca tcatagcgtg
     6781 gtgcagtttg cccatgctca gggcgcgtac cggcaaatcg atgtcggcgg cgtttacggt
     6841 tttgccgctg gaggcggtgt aatcggcggc gggcgcgacg aaggcgggtt tcggcgtgtg
     6901 cgcgcgggcg gcggcttcgg atacgtcgct gatcaaaccc attttcagcg cgccatatgc
     6961 gcggatggtt tcaaattttt ccagcgcggc ggcatcgttg ttgatgtcgt cctgcaactc
     7021 tttgcccgtg tagcccaagt cggcggcgtt caggaaaacg gtcggaatgc ccgcgttgat
     7081 gagcgtggct ttcagacgac ctatattcgg cacatcaatt tcgtcgacca aattgccggt
     7141 tgggaacata ctgccttcgc cgtcggctgg atctaaaaat tcgatttgta cttcggctgc
     7201 cggaaaatta cgccgtcaag ctcgaaatcg cctgtttcca acactgctcc gttttgcatc
     7261 ggcacatggg caataatggt tttgccgatg tttttctgcc agattttgac cgtgcagatg
     7321 ccgtctgaag ggattttgga tttatcgacc aagccctgtt cgatggcgaa tgcgcccacg
     7381 gcggcggtaa ggttgccgca gttgccgctc caatcgacaa atggtttgtc gatggaaact
     7441 tgtccgaaca agtaatcgac atcgtgatcg gcgcgttcgg atttgtccga aatcacagct
     7501 ttgctggtgg acgaactggc gttgcccaaa ccgtcaatct gtttgccgta agggtcgggg
     7561 ctgccgagta cgcgcaagag gattttgtcg cgcgcctcgc cttcttgttg cgcagcggcc
     7621 ggcaaatcgg tgcgtttgaa gaaaatgcct ttagatgtac cgcctcggta gtaaacggct
     7681 ggaattttaa tctgcggcat ttcgagttct cctttgtatt tttaatatgg tttttcagac
     7741 ggtctctcaa acattcgagg ccgtctgaaa acctttttga gcgcagcccg gacattttga
     7801 cgatacccga ttaccttacg ctgcatttcc ttctaaaaaa tcttgtgcga aacgttgcaa
     7861 cacgccgccg gcttcatata ccaatgcttc ttctgcggta tcggggcggc aggtaaccgg
     7921 aacttcgacg gtttcgccgt ttttacggtg aatcacgagg gtcaggccgc agcgcggtgt
     7981 gcgttcgccg acaacgtcgt aggtttccgt accgtccagt tgcagggtat ggcggttggt
     8041 gccgggtttg aattgcagcg gcaagacgcc catgccgatg aggttggtgc ggtggatgcg
     8101 ctcgaaacct tcggcggcga tggcttccac acccgccagc cgcacgccct tcgccgccca
     8161 gtcgcggctt gagccttgac catagtccgc accggcaatg atgataagcg gctgtttgcg
     8221 gttcatatag gtttcgatgg cttcccacat gcgcatggtt tggccttctg gttcaacgcg
     8281 tgccaacgaa ccttggcgta cgctgccgtc ttcgtttctc accatttcgt taaacagttt
     8341 cggattggcg aaggttgcgc gttgggcggt caagtggtcg ccgcggtggg ttgcgtaaga
     8401 gttgaagtct tcttcaggca aacccatttt cgccaaatat tcacctgcgg cactgccggc
     8461 caaaatcgca ttggatggcg agatgtggtc ggtggtgatg ttgtcgggca aaatcgccgg
     8521 cggacgcata cctcttaatg tacgttcccc tgccagtgcg ccttcccaat agggcggacg
     8581 gcggatgtag gtggacatcg gtcgccagtc gtacagcggg cttggtgctt tttgcgctgt
     8641 gccggtgtcg gacatcggga tataaatgtc gcggaattgt tgcggtttca catattcggc
     8701 aacgatggca tcgatttctt cgtctgtcgg ccagatatct ttcaggcgga tttcgcggcc
     8761 gtctgcaacg ccgagtacgt cgttttcaat atcgaaacgg atgctacctg ccaatgcgta
     8821 ggcaacgacc aaaggaggcg aagcgaggaa agcctgtttc gcatacggat ggatacggcc
     8881 gtcgaagttg cggttgcctg acaatacgac ggtggcgtac aaatcgcggt cgatgatttc
     8941 ttgttggatt ttcgggtcga gcgcgccgct catgccgtta caggtggtac atgcgaaggc
     9001 gacgataccg aagccgagtt tttccatttc gggcaacaag cctgcttctt tcaaatagat
     9061 tccggctact tttgaacccg gggcaaacga agatttcacc caaggtttgc gtttcaagcc
     9121 gaggcggttg gcattgcgtg ccaacagtgc ggcggcgaca acgttgcgcg ggttggaagt
     9181 attggtacac gaagtaatcg cggcaataat cactgcaccg tcaggcattt ggccgtctga
     9241 aggctcttcg taaggcttcg ccagcccttt cgccgccaaa tcggcggtgg caaaacgcgc
     9301 gtgcgggttg ctcgggcctg ccatattgcg cgttacgctg ctcaaatcaa atttcaaaac
     9361 gcgcggataa acggcggttt tcaagccacc tgcccataag cctgcggttt tggcgtaggt
     9421 ttccaccaat ttcacctgcg cgtcgtcacg tccggtcagt ttcaaataat caatagtttg
     9481 cgcgtcgatg gcgaacatgg cggcagtcgc gccgaactcc ggcgtcatgt tggaaatggt
     9541 cgcgcggtcg ccgatagaca ggcttctcgc gccctcgccg aaaaattcga caaacgcccc
     9601 gaccacgcgc tctttacgca agaattcggt cagtgccaac acgatgtctg tggcagtaat
     9661 gcccgcctgc cgtttgcccg tcagctccac gccgacaata tcgggcaggc gcatcatgga
     9721 cgcgcgtccc agcatcacgg tttccgcttc caaaccgccc acgccacgga aatcacgccc
     9781 agcgcatcaa cgtgcggcgt atgcgagtcc gtgccgacgc aggtatcggg gaaagccacg
     9841 ccgtttttga cttggacgac gggcgacatt ttttccagat tgatttggtg catgatgccg
     9901 ttgcccgccg gaatcacgtc cacattttca aatgcggttt ttgtccagtt gatgaagtgg
     9961 aaacggtctt cgttacgtct gtcttcgatt tcgcggtttt tgcggaaggc atcaggatcg
    10021 taaccgccgc actccaccgc caaagagtgg tcgacgataa gctgcgtctg caccaccgga
    10081 ttcactttgg caggatcgcc gcctttttcg gcaatcgcat cgcgcaagcc tgccaaatcc
    10141 accagcgcgg tctgccccag aatatcgtgg cacaccaccc gcgcgggata ccacggaaaa
    10201 tcgatttcct gcttcccttc tatcaactgg cccagccagc tttgcagcat cggcaaatcg
    10261 actttgtccg cgcggttgac caagttctca gacaaaatgc ggctcgtgta cggcagcttg
    10321 tcgtaagagc cgggtttgat gtcctcacac gccgcacgcg cgtcgtagta ttccaaatcc
    10381 gtaccgggca gcggtttgcg gtaactttgg ttggcagtca tgtcggttct cctgtggatc
    10441 tatttttctt gtggtttggg tttcagccga tgttttgaag gagtcgtctg aaagggttta
    10501 aaacatcgaa acaatcatct gatacagcgg atttctctcg tctatcaaca atttcacagc
    10561 catcgaaatg ctgatgacaa tcagcagcgg cttaatcagc ttcgaaccga agcggacggc
    10621 aaatctcgca cctaaattcg caccgacaaa cgcaccgacc gccatcgttg ccacaatcgg
    10681 gaaaataatc gaaccgtgca gcaggaatac cgatagcgaa ccaagattgc aagcaacgtt
    10741 cgccaatttg gtgtaagaca tcgcgttcaa cagcttgcag ccgagcaaaa caataaaggc
    10801 aatcagaaaa aacgagccga cacccggtcc gaacacaccg tcgtaaaaac ccaaaagcgg
    10861 tgcaaccgtc agcccgaata gaaaaaaaga cattctggct ttgccttcct tactgccgtc
    10921 gagcttgggc gaaaacacaa aatacagcgc gacaaatatc aacaaaaccg gcacgaccgc
    10981 cagcaaaata tctttggaaa ccaagctgac cgataatgca ccgaccacgc cgcctgcaaa
    11041 cgatgctgcg gcaatcggga gacttttctt ccaatcaatc aaacctttgc gtgcaaaaga
    11101 aaccgtagcc gaaaacgtag cagcggctgc ttgcagcttg ttggtggcaa ttgccgacac
    11161 gggaggaata cctgccaaca agagtgcagg cagcgtaatc aaaccacccc cgcccgcaat
    11221 cgcatcgata aatccggcga tcatcgcaac caaacccaaa gcgagtatta tgtataaatc
    11281 ttccatgttt cttatcctgt tacttgcgcc aatacaggat tatcttctcc tattagatta
    11341 aacttatttc agacaacctt tccaataagg caaggtcgtc tgaaatcctc agctttgcat
    11401 accgaaataa agaacaaact gctattgcat tcttcaatta tcgttcttca atctccacaa
    11461 acgccaaatc ttcagggccg gtgtagtttg cgctcggacg gatgattttg ccgtctttgc
    11521 gttgctcgag gacgtgtgcg ctccaaccgg ttgtacggga aatcacgaac agcggtgtga
    11581 acatagcggt cggtacgccc aatttttggt aggaaacggc agagaaccag tccagattcg
    11641 ggaacatttt tttctcttcc cacatcacgc tctccaagca ttcggcgatg tcgaagaggc
    11701 gcatgtcgcc cgcttcccgg ctcaaaccgc gcgccacttc tttaatcacg acgttgcgcg
    11761 ggtcggaaat ggtgtaaacc ggatggccga aaccgatcac gatttctttg cggccgatgc
    11821 gttcgcggat gtcggcttcg tcggcgttgc ggtagcgttt ttggatgtcg taggcgactt
    11881 cgttcgcacc gccgtgtttc ggacctttaa gcgcgccgat ggcgccggtg atgcaggaat
    11941 acatatccga acccgtaccg gcaatcacgc gggcagtaaa agtggaggcg ttgaactcgt
    12001 gttcggcata cagaatcagt gaaacatgca tggctttgat gtgtgattcg cttgggcgtt
    12061 tgccgtgcaa caggtgcagg aaatgaccgc cgacggtctc ttcgtcgctt tcaacttcaa
    12121 tacgtttgcc gttgtgcgaa tattgatacc agtagagcag gatgctgccg aggctggcga
    12181 tcagtttgtc ggcgatgtcg cgcgcttcgc tgtcgggatg gctttcgcgt tcgggatgaa
    12241 cgcagcccag catggatacg ccggtacgca tcacgtccat cggatgggta tgtgcaggca
    12301 gggtttccaa aactttaatc acacggatag gcaggccgcg catggatttg agcttggttt
    12361 tataagcggc cagctcgaat ttgttgggca gatggccgtg aatcagcaag tgtgcgactt
    12421 cttcaaactc gcatttttgt gccaaattta gaatgtcgta accgcgatgg ctcaaatcgt
    12481 taccggtacg gccaacggta cacaaagcgg tattaccggc cgcaacacct gaaagcgcaa
    12541 cggatttttt aggtttgaag gtcggggttt gagtagtttc agtcatggta tttctccttt
    12601 gtatttttat gggttttatt tttcagacgg tcgatgcgga tttgttgaaa ggccgtctga
    12661 aagcggtaaa tcatttttga aacaatttat ccagtttttg atcgaaggca tgatagttca
    12721 gatgctcgta cagctcggca cgggtttgca tactgtccac caccgccgcc tgagtgccat
    12781 cgcgcataat cgcttcgtaa acattcaaag cggctttgct tgctgcgcgg aatgatgaca
    12841 acggatacag caccagtgac acgccgtttt cagccaactc gctttgcgta taaagcggtg
    12901 tagcaccaaa ctcggtaatg ttggccaata ctggtacttt cactgcatct gcaaattgac
    12961 ggtacatttt caaatcggtc atggcttcag ggaaaatcat gtccgcaccg gcttcgacac
    13021 aagcctgagc gcgttcgata gcggcatcca aaccttctac cgccagcgca tcggtacgcg
    13081 ccataatcac gaagttctca tcaacgcgcg catctacggc agctttgatg cggtcgacca
    13141 tttcatcttt ggaaacgatg gctttgttcg gacggtggcc gcagcgtttt tgcgctacct
    13201 gatcttcgat gtgaaccgct gcaacaccgg cgcgttcaaa gttgcgaatg gtacgggcaa
    13261 tattgaatgc accgccccaa cccacatcga tgtccaccat cagaggcgta tccacgttgt
    13321 ccgtaatgcg tcgtgcgtcg atcagcacat cttccattgt ggtaatgccc aaatcaggga
    13381 taccgcaaga acaggctgcc acgccgccgc cggacagata gatggctttg aaaccgcttt
    13441 gggtggccaa tcgtgcaaag taagcattga cgcaaccgac gacggcaagc ggattcgatt
    13501 ctttcacagc ttggcggaaa cgtgctccgg cagagtgttg actcatcata tttctccttt
    13561 atagactttt ttcagtatgg acaggcttcc atcacattcg gacggcaaaa cacagccatc
    13621 cgacgcgtcg ggcatcccaa tcattaaaaa tatatgggaa aaattatctt attgatattt
    13681 aaaacgaatc aaagaaaaac agcagaccgt tcggaattat gcggcaaaac cgcagacaag
    13741 aagaaaacaa ggggattatt cagaaaaggg aaaaacatct gaattggttt catagtaatg
    13801 ttcctttgta gtattatgta gttttatttt tcggtaatct aaaacacaag actggaatgt
    13861 caaacatttt taaggagact aattacataa ttttattatt ttattttaac attatgatta
    13921 atataaatta attttaactt atttgtttcc gataaaatta ataggaaatt tagaaaaatt
    13981 ttcaataatc aaaatcctga aaaattataa aatattttat atataaaaca atgaattatt
    14041 ttcaagtaat ccgatgtagt ttattatcta tttgattttt atattattta tttaaaatta
    14101 aatatatctg gcaaacaaaa tgccgtctga aaacctttca gacggcattt ttaatatcga
    14161 atcaatcagc ccaaatacca gaatttcaat gcccacaata cggcaacaac ccataccata
    14221 ggcggcacgt ccccagtccg gcgacacaaa agtttgacca cggcatagct gatgaagccg
    14281 aaggcgatgc cgtctgcaat cgaataggta aacggcatga agacaatggt caggaacgcg
    14341 ggcgcggctt cagtcatatc gtcccaatca atgtccctcg cactgcggag catctgcgtg
    14401 ccgacataaa gcagtgcggg cgcggtggca aataccggaa cacttttcgc caatggggag
    14461 aacatcagac acgccagcat caatacgccg acggtaaccg ccgtcaggcc ggtccgtccg
    14521 cctgccgata cgcccgccgc gctttccaca taaggtgtgg ttgaagaagt acccaaagcc
    14581 gcacctgcca caatggcggt agagtctgca agcagtgcgc gtttcaggcg gggcagctta
    14641 ccgtccacca gcagtccggc acggtgggat acgccgacca gcgttccggt actgtcaaac
    14701 aaatcgacca agaagaagac gaaaatcacg ctgaccatgc tgacggtaaa cagaccttta
    14761 aaatccatct gcataaaggt cggcgcaatg cccggtactt cgccgaccac gccgtgaaac
    14821 tcgttcaaac ccatcaggct ggcaatgacg gtaatcgtca gaatggtgat gatgattgcg
    14881 ccttgaacgc ggaaataccc caatacgacc accatgacaa aaccgaacaa tgccaacagt
    14941 gcgctgggct gatgaatatc gcccaagccg accaaggttg ccggattggc aacgataatg
    15001 cctgcgcctt tcagggaaat cagtgccaaa aacaaaccga taccggcggc aatcgacatt
    15061 ttcaaaccca taggcagtgc gttgaccagc atttccctga ctttaaaaaa gctgaacagg
    15121 atgaaaatca gaccggaaat gaacaccgca cccaacgcca cctgccaagg cacgcccata
    15181 cccttaacga cggcaaaggt gaaataggca ttcagcccca tccccggggc aagcgcaatc
    15241 ggatagttgc cgataaaacc catgacaaaa cagccgatgg cggatgcgat acaggtggcg
    15301 acgaataccg cccccatatc cattccggtc tcgcccaaaa tcaggggatt gacgataacg
    15361 atgtagcaca tcgtcaaaaa ggtcgtcaaa cccgccatca actcggtacg taccgtcgta
    15421 ccgtttgcct tcaggttaaa aaccctatcc agcaatgttt gttttgaaat atccatatct
    15481 agcagtccgt ttcaagtaaa aaatctgaac ccgcacaccg caaaccgcac aggattcggc
    15541 atattgaaaa atatacctcc caaatcggga aaaccggttc gggaggcaat aagtttacgg
    15601 cagacggcac attatttgtc tgaattgtgt tgacgacgca aaattgcagg aatttcaaaa
    15661 tcgtcaagga cagactggtt atcgaaatcc gctgctgtca gattcatggt gcggataccg
    15721 cgattggtgc ggatcatacc ttcgacattg tggctttgct cctgtttgga cggggcaacc
    15781 gcttctactt cccttgccgg agtaggatcg accgctcctt tttctttcag accggtagcg
    15841 ataatggtaa tccggatggc atcttcgctc atggtctcgt cttcagcggc accgaatttg
    15901 cattccaaat cgggatgcgc gctttggttg acgattttca tgacttcaga caactcggac
    15961 attttcaggc aacccggagc agtcgtaata ttgaccagca caccgcgtgc accatccaaa
    16021 gttacatcgt ccagcagcgg actggaaatg gcctggtcgg tcgccatacg cgcacggtcg
    16081 ataccttggg cataacccga acccatcata gcgataccgc ggttgctcat cacggttttc
    16141 acgtcggcaa agtcgaggtt gatgatttcg ctcgggcaag ttaccacttc ggaaatgcct
    16201 gcgaccgcat cgcgcaatac attgtccgcc gcgcggaagg cttcgcgcat agttacgtct
    16261 tcacccaatg cggtcatcag tttgtcgttc gggatgataa tcagcgaatc gacgtgttct
    16321 ttcaactgtt ccaaccctgc ctgtgccaca tggacgcgtt tgccttcata agaaaacgga
    16381 cgggtaacta cggcaacggt cagaatgccc aaagacttgg caatctcagc aacaaccggc
    16441 gcggaaccgg taccggtacc gccgcccata ccggtcgtga taaacagcat attggcaccg
    16501 cgaatggctt cttcaatggc ctcccgatct tcctgtgctg ccgcacggcc gatatcggga
    16561 ttcgcacccg cgcccaaacc gcgtgtcaga ttcgtaccca actggattct cttcgccgca
    16621 tggttttttg ccaaagactg cgcatctgta ttggcactga taaactccac actgcgcaca
    16681 ttgttggcaa ccatgttatt gattgcattg caaccgccgc cgcccaagcc gattacttta
    16741 attaccgcag ggctgactgc cgattctgcc acgtcgtaaa caaattccat tcaaaaactc
    16801 ctgatcgccc cgttcagagg acggtttaaa taaattatta tttattatat aagaagtatc
    16861 ttactgctgg caaaatactt ctcacctgtc aaacggcaat ccaactgttc agaggttgtt
    16921 ttcaatccac cgcttcaatc ttgccaacaa accgccgccc ccttctctct cttgcactgt
    16981 accgttttcc ggttgcggca agtttccttc cagcttgcat gctgcatgaa gcagcccgat
    17041 agcggtagaa aagcgcggtg tgcggacacg gtcggacaaa ccgcccattt cttggggcgc
    17101 accggtgcgt acaggcaaat cgaagatttt ttcggcaaat tccacaatcc cggtcatcat
    17161 ggacacaccg ccggtcagaa cgatacccgc attcagcact tctttgggga aacccgattt
    17221 ttgcagttcg cccagcacta cgccaaaaat ctcctgaata cgtgcgctga tgattgctgc
    17281 caaaacctta ctggaaacct gacgcgatgt ccggtcaccc acgcccggaa cttcaatcat
    17341 ctcgcccaag ccttccgtat cgcatgatgc cacgccataa tggattttaa tgtactcggc
    17401 ggcatcgaga ggtgttctca acgatttgga caaatctttg gtaatcagat taccaccggc
    17461 cggaatgacg gacgtatggc ggatggcgcc gttcatataa acggcaatat cggtcgttcc
    17521 gccaccaatg tcaatgacgc atacgccgag gtctttttca tcttcagtca gcaccgcctg
    17581 cccgcttgcc aacggttgaa gcatgatctg atcgcttttc aagccgcaca gctcgataca
    17641 tttttggaca ttctgcactg ccgtacttgc accggtaatg atgtgcaccc gcgtatccag
    17701 acgcacaccg ctcataccga tgggctccct tacaccaagt tgggtgtcga taatgtagtc
    17761 ttgaaccacg gcatcgagaa ttttttgatc gggcgggata ttgattgcct ttgccgtttc
    17821 aatggcgcgg tcgatgtctg cctgtgttac ttctccgtct ttgattttaa ccacaccttg
    17881 cgaattaagg ctgcggatgt ggttgcctgc gatacctgtg gtaacgtggg taattttggt
    17941 atccgccatc agctcggcat cattgaccgc ctgcctgatg gcttggacgg tggcatcgat
    18001 attggttacc atgcccgcgc gcaagccccg tgaaggagcc tgccccaaac cgatgatgtt
    18061 gattttgtcg tcatcttgaa cttccccgat cagtgcgagg actttagacg taccgatatc
    18121 cagtacgctg atgtatcttt tctgctgttc cattgttcgt ctgcccttaa aactgattga
    18181 aatttgcgtc gcaccgtttc agacggcacg gccgtaatat gtccgatacc tgttcccact
    18241 attcttcgga ttctttttcg ggtaaaccgt cgggagcatg gcggactgaa aatccgtcct
    18301 tatacctcat atccacatag gataaccgat ttttattctt acgcaacaga tgctgccacg
    18361 cttcggtaaa aagccggagg cgtttcatct cgttttcccg tccgagcctg acggtgatgc
    18421 cgttgtccaa aacgacattc cacgccgaac gtgccgtata ggtcatctct ttgatgccca
    18481 aaccctgttt tgccaaaaca gtcgaaaatt cgtcataacg gcggagcatt tcggcagacg
    18541 ttccttccgc gcctctgaat accggcattc cgggtctgtc caagcgggct tcaaaaacat
    18601 tgccttcgcc gtccaccaag gcatggtcgc cccaacgtgc aaccggcttg cgctcggtca
    18661 ggacgacctc aaccgtatcg ggaaaacggc ggcgcaccat gaccgacgca atccacggat
    18721 accggcggta ggcttcctgt gcgccattga tgtccgtcct caaaatattc ccatggatgt
    18781 attctttcgc caaactgccc aatgccttct tatcggaata aaccaggttg cccttcagcg
    18841 acacctgctt gacgggcaga tgattcgaat tgtaaaacca aaccagcccg gacgcagcaa
    18901 gcagcatcgc catcatgaca agcagccagc gcgtcagccg ttccatcgct tcggcattat
    18961 cccacatgtg cggccttcaa aatttcaata cataaatcgg caaaacccac gcccgtaacg
    19021 gcagcggatt tcggtactaa actatggccg gtcataccgg gcagggtgtt gatttccaac
    19081 agatagagtt tgccgtcggt atctttgagg aaatcgacgc gcacgcagcc ttccgcaccg
    19141 attgcctgtg cgccgcgaac cgccagttcg cgcatcaggc tttcttcggc ttcggtcaaa
    19201 tcttccgaag gacattgata aatggtgtcg tctcggttgt acttggcttc gtagtcgtaa
    19261 aactcggttg cggggatgat gtgtatgccg ggcagccctt tgccgttcag gacggggcag
    19321 gaatattcgc cgccgccgat aaaacgttcg gcaatgattt cgccctgaag gtgtttcaat
    19381 tcttcgtaaa cgcttttcag acggcctttt tctttgactt ttaccacgcc gacgctgctg
    19441 ccttcgaccg ccggcttcac aaacatcggc agacccaatt tttcttcgac ggcatcgaaa
    19501 tcggtatcat cgtacagtac ggcgaactcg ggaacgggta atcccaatgc ctgccaaatc
    19561 agtttgcagc ggtatttgtc catgccgatg gcggaggcgg cgacaccgct gccggtatag
    19621 ggaatgccca acagttccaa tgcaccctga acagccccgt cttcgccgta agtaccgtga
    19681 aggatgttga atgccgtctg aaagccccgc tccttcagtt cggataacgg cgtttcctta
    19741 gggtcgaagg cgtatgcgtc tatgcctttg ctttttaagg cgttcaaaat ggcggtaccg
    19801 ctgtccagcg agatttctcg ttcgctggaa aaaccaccca tcaatacggc cactttgcca
    19861 aaattctgca ttgtttttgt tctttcctaa ttgctttatg cttgttgtca gaggtcgtct
    19921 gaaacctgat tgcggtttca gacgaccttt atatgatgtt ccgtctgtca ggcgggtgta
    19981 tctcaaatct gtttcgacaa ttccaacagc gcggagggaa cgcggttgat gcttcccgca
    20041 cccatattca acacaacatc gccatcctgt aaaacattca tcagcatttg cggcaggtcg
    20101 gcgacgtttt cgcagtaaat cggctcaagt ttgcccaata cgcggatagc acgcgccaag
    20161 gcgcgggagt cggcggcggc aaccggctct tcgccggcgg cataaacttc ggtcagtacc
    20221 agcgcatcaa cggtattgag tactttggta aagtcttcaa acaaatcgcg cgtgcgggta
    20281 tagcggtgcg gctggaaggc gagtaccaaa cgtttttccg gatacgcgcc gcgtgcagcg
    20341 gcaagggttg ccgccatttc gacggggtgg tgtccgtaat cgtccaccag caaagcggtc
    20401 ccgccgtttg gcaacttgat gtcgccgtat ttttggaagc ggcggccgac gccttcaaag
    20461 ccgagcaagc ctttttggat cgcttcaacc gatgcgccga cttccagcgc cacgccgatg
    20521 gctgccaatg cgttcagcac gttgtgtctg ccgggcatat tcagcacgac ttcaaacgac
    20581 ccctgctcat gtcctttcat ttgaacatgg acggtgaatt tcatttgcgc gccgacgttt
    20641 tcgatgtcgg tggcgtagat gtcggcggta tcgtccaaac cgtaagtagc ataaggtttg
    20701 ctcactttgg gcaaaatcgc gcggacgtgt tcgctgtcaa cacacaaaaa ggctttgccg
    20761 tagaagggca tacggtggat gaaatcgata aacgcctgat gcagtttttc gacgctgtgc
    20821 ccgtaggtat ccatatggtc ttcgtcgata ttggtaacga cggacataat cggggtcaga
    20881 tgtaggaaag aggcatcgga ttcgtcggct tcggcaacga tgtattcgcc tttgcccaag
    20941 cgggcgttgg tgcctgcggc gttgagtttg ccgccgataa cgaaagtggg gtcgagtcct
    21001 gccgcgccga ggatggaggc ggtcaggctg gtggtcgtgg ttttgccgtg cgtaccggca
    21061 atggcgatgc cgtcacggaa gcgcatcagc tctgccagca tcaaggcgcg cggaataacg
    21121 ggaatttgcc gctccaacgc agcgacaact tcgggatttt ctttcttgac ggcggtagag
    21181 gcaacgacga catccgcacc gttaacgtgt tctgcggtat ggccgggata aacttgaatg
    21241 cccaggctgc tcaaatgctc ggtagcggca tttcgcgcct gatccgaacc ggaaacttta
    21301 aagcccaaat tgtgcaagac ttcggcgata ccgctcatgc cgacgccgcc gataccgaca
    21361 aaatggatgt tgcttactcg atttttcatc ataatgttgc gttccggttg gtttttagtg
    21421 cgtaaaggcg ttattttaaa ggtctgaccg tttgcgcacc atagttttct gacaaatata
    21481 tagcggattg aaataaaaaa catccatgcc gtctgaacgg cttttcagac ggcatggttc
    21541 ggcagtttac gccgcacacg caatcgcggc ttcggcaacg tcatccgcgc tgtgcggcaa
    21601 tgccaacgta cgggcgtttt ccgcccattt gaggcatttt tcgcggttga ggctgccgag
    21661 gatttcggcg agtttttccg ccgtcaactg ggtttgcggc aacagcagcc ccgcttctgc
    21721 ctgcaccatg aaacgcgcgt tggcggtttg atggtcatca acggcgtgag gatacggcac
    21781 taacaacgcg cccagccccg ccgccgtcaa ctcggcaatc gtcagcgcgc cggcacggca
    21841 aatcaccaaa tcggcatcac ggtaggcgga caccatgtcg gtaataaatt ccacgcattc
    21901 cgctttcacg cccaacgcgt cataatccgc ctgaagattg cccagcttgt tacgccccga
    21961 ctggtggtac atctgcgggc gcacctcttc aggcagcagt gccaacgcct gcggtacggt
    22021 tttgttcaaa acgtccgcac ccaaactgcc gccgaccacc aaaattttca gacggccttc
    22081 gcgcccttgg aagcgttcgg caggcacggg caggttgcta atatcggcgc ggacggggtt
    22141 gccgaccaaa ccgccttcgt ggctgaacgc tttcggaaaa gcatacaaca cccgtttcgc
    22201 ccagcgcgac aggtggcggt tggacaagcc tgccacggcg ttttgctcgt gaatcacaat
    22261 cggcacgccc aagagtttcg ccgccagacc gccgggaaag gtaacaaaac cgccgaagcc
    22321 gatgacgcac tcgacacggt gtttgcggat aatccgctgc gcttcgcgga cggttttgta
    22381 cagagtaaac ggaagcatca gcttgcgttt gatgccgttg ccgcgtattc ctttaatcgc
    22441 cagcgtttcc aagcgtatgc cgtattgcgg cacgatgcgc tcttccatcg aatccttgct
    22501 gcccagccaa attacatgat gaccgcgcac gcgcaatgaa tccgccacag ccagagctgg
    22561 gaaaatgtgt ccgcccgttc cgcccgccat cagcataaag gttttaccgc ccatgattta
    22621 ctccacccgg taaccgcgca ttttccggcg gttttcataa tcgatacgca acagcagcat
    22681 catgctgatc agcatgaaaa agactgacga accgccatag gacatcaacg gcagcgtcag
    22741 acctttggtc ggcaaagcac cgatgttcac accgatattg aagaaacttt ggataccgat
    22801 ccaaatgccg atacccgaag cgatataggc gttgaaagtc aaacccaaat cgcgcgactg
    22861 cttgccgatg gaaaacgccc gcaccaccag ccagccgtaa cagaatatca gcacgcacat
    22921 cccgaagaag ccgaattctt cagcgatgat ggcaaaaata aaatcggtat gcgcttccgg
    22981 cagaaagccg cgtttgctca aactcgcacc caaacccata ccgaaccact ctccgcgccc
    23041 gattgccatc agagagtggg taagctggta gccggcaccc tgcgggtctt tccacgggtc
    23101 caaaaatgcc actacccgct gcacacggta gggagcggcg gtaatcatca gcaccatccc
    23161 acccaagacg ctgcctacca ggacaaaaaa atatttccac ggcaatcctg ccagaaacag
    23221 cattccaacg gtaatgacgg taatgacgac aaacgaaccg aaatccggct gtaccattat
    23281 cagcaccaaa ccgaacgcca ccagcataat cggcaggatg atcgcccgga aacggccgta
    23341 catttctaat gtttcacgac gtgcctgcgg attggtggcg gacataatca ggttggccgt
    23401 cccccgccaa atcgactgcc aacccaaact ttccatgctg cgcaacactt cttcacggcg
    23461 cgtgaacagg cttgccaaat aaaggatgac tgccagcttg aacagctcgg tcggctggaa
    23521 attcaacgga cccaaaggta tccaacgggt cgcgccattg atttcgcgcc cggcaatcaa
    23581 tacggctacc agcaacaggc cggataaggc aaaaatccac ggcacaagcc gccgccatgt
    23641 cctcatcctg caaagaaacc ataaaaaacc gctcgctata aggccggcaa cgacgaaccc
    23701 cgcctgcctg gtcaaataga aaaactgatc gccgccttcc ttcgatgcca aatacacaga
    23761 agccgaataa atcatcagca ggccgaacgc cgtcatcagc accaccatcc acaaaagcgg
    23821 cgcgtcgaat ttcctgccgt cgcgcacaat cggcctgtcg agcagcagag tgtggacacc
    23881 gtcgcccact tttaccaata cttccgaaat cttcaaaaaa ccacctgcca gtctgtttgc
    23941 acctgccgca aagggcaaaa atttcagacg gcagacaatg ccgtccgaac atacgataca
    24001 tcccaaatcg gtattctaaa tctttacttg ccgcccaaca atgacggcgt ttgtgtttcg
    24061 gacggcatca caaagcctta aacgcttcga taaacacttc cgaacggtgc gcgtagcctt
    24121 taaacatatc gaaactcgcg caggcggggc tgagcaagac aatatcgccc gcttcggctt
    24181 gggcgtatgc cgtctgaacc gcctcttcca aagtgacgca gtcggtcagg ttcaagccgc
    24241 agccgtccaa atcgcggcgg atttgcggcg catcgacgcc gatcaggaac acgccttttg
    24301 ccttatcttt caacgcgtcg cgcaggggcg tgaagtcctg ccctttgccc atgccgccca
    24361 aaatcacgaa gagcgggttt tgcaaaccgg caatcgcggc ggcggtcgcg ccgacattcg
    24421 tgcctttgct gtcgtcgatg aataccacgc cgtttttctc gccgattttt tccacacggt
    24481 gcggcaagcc ttggaaggtt ttgacgtgtt ccagcaatgc ttcgcgcggc aaaccgacgg
    24541 cctcgcacaa agccacggca gccataacgt tggcggcgtt gtgcaaacct tgcagcggga
    24601 tgtcttgcgt agaaatcaaa tcttcattgc cttgttttaa acagcccgtc ccgcgttcca
    24661 accaaaaatc ggcttcgtgt tccaaggaaa accgtttcac ttcacgccct gcccgtttca
    24721 tggcgcggca gaacacgtcg tccgcattca aaacctgcac tccatcgcca cggaaaatct
    24781 tggctttggt atgcgcgtag tcgagcaaat cgtcgtagcg gtcgagatgg tcttcggaaa
    24841 tgttcatcac cgtcgccgca gtcgggcgca ggctttcggt gttttccagt tggaagctgg
    24901 aaagctccaa cacccacacg tccgcctttt tgccttcgcg ctgcaattct gcctccaaaa
    24961 ccggcgtacc gatattgccc gcaatgacgg tatccagccc gcacttgatg cagagatagc
    25021 cgaccaggct cgttaccgtg gttttgccgt tgctgccggt aatcgcaatc accttgtcgc
    25081 cgcggcggtt cacaatgtcc gccagcaatt cgatgtcgcc tagcacgcgc ccgccgtttt
    25141 gcttgaacgc ctcgatatcc ggctgccgct cgctgatgcc gggactgagc gccagaatat
    25201 cgaaaccgtt gtccaacgca tctttcagac gacccgtgta aaacaccagc ccgtcaaaca
    25261 tcttaccgat ttgcgccacg cgctcggctt tcagctccgc atcataagcg gcaacctccg
    25321 cgccgttttt gcgcagatag gcaatcatgg aaatacccgt gccgccaagt ccggcgacga
    25381 gggttttttt gttttggaaa gtcattttgg ttttgtccta aaacaaatca tattgggcag
    25441 gagacgtccg cccttgccca agccgctttc agacggcatc gcgagccgat taataacccg
    25501 ccttcaggcg ttggtcattg tcgcagctgt tttggtctcc gttttgacaa gccttgccaa
    25561 gccattgttg agcgagcgca aggtcttggc gcacgccgcg tccatcgtaa tacatcaagc
    25621 ccaaattgta ttgggcttgg gcatcccctt gttctgaagc cttgcgatac cactgcactg
    25681 cctgtacata atcttgacga actccttgtc cattttcata catcacgccc aaattgaatt
    25741 gggctgctgc atttccctgt tctgccgcct gcaagttttc ccgaaaatcc gatacgtcac
    25801 ccgcccacac cgcttggttc aagcccaagg caatcagggc ggcggcaagc catttgactg
    25861 tctgtttcat ggttttctcc tgttttagta taaggcgggt ttcagccgcc gttaacgata
    25921 ggacggggcg gattgccgcc gcaggtttat tgcgcgttca aatgccgtct gaaaggtgtt
    25981 cggacggcat aggttcagcg aattttgagg gtactcaaac cgatcaatac caagacgatg
    26041 gtaataatcc agaaacggac gacgacttgg gtttctttcc agcctttttg ttcgtaatgg
    26101 tgatgaatcg gcgccatcag gaagatgcgt tttttggttt tcttgtacca gccgacctga
    26161 agcataacgg acacggcttc tacgacgaac agaccgccca taatgacgag gacaaattct
    26221 tggcggacga tgacggcaac ggtaccgagc gcggcaccca atgccagcgc gccgacatcg
    26281 cccataaaga cttgcgcggg ataggcgttg aaccacaaaa atccgaggca cgcgccgcac
    26341 atggcggtgc agaatatagc gacttcgttc gcgccggcga catagggaag ctggaggtat
    26401 tgggaaaatt ggtagtgtcc gctgacgtag gcgaaaatgg cgagcccggc ggcaacgagg
    26461 acgacgggga aggcggcaag gccgtccaag ccgtcggtga ggttgacggc gttggatgtg
    26521 ccgacgatgg tcaggtaaga caacaccaaa aagccgacca cgcccagcgg cagggcgatt
    26581 tgtttgaaaa acgggacaat caaaatattg ttggcggaat tggcggcaag gtaaaacaat
    26641 gccaaaccgg cgataacggc aacgcttgac tgccacacca ttttgaattt ggcggacacg
    26701 ccgttggggt ctttataaac gactttgcgc cagtcgtcgt aaaaaccgag cgcaccggtg
    26761 gcaagcagta cgcccaagag aatccagata tacgggttcg cccagttgcc ccacaacagg
    26821 gtggacacgg taatggcggt cagaatcagc gaaccgccca tcgtcggcgt gccgtttttg
    26881 acgaggtggg tttgcgggcc gtcggtacgc actgcctgcc cgcatttgag cgcggtcagc
    26941 ctgcgtatcg tccacgggcc gaacatcagg gaaaacgcca aggcggtcaa cgccgccata
    27001 acggcgcgga atgtggtgta ttgaaaaata ttcagaccgg ttaaccagtt gctgaaatgt
    27061 gcgagccata aaaacatggg gcttcctttt ttgttttgtc gttatagcgg attaacaaaa
    27121 accggtacgt cgttgccccg ccccggctca aagggaacgg ttccctaagg cgcccaagca
    27181 ccgggcgaac cggttccgta ctatccgtac tgtctgcggc ccgccgcctt gtcctgattt
    27241 ttgttaatcc gctatacatc gggcttgcgc ccgcttgagg tttgcttgct gtccggcaag
    27301 gtttcggacg gcttttgcag attaacgtgc agggactttc ccaatcacgc aaatcgggta
    27361 gctccccgga tttttgccgc cgcccaagtc taaacataaa tcttcataaa gatacgcctg
    27421 cgtccgcatt ccggcataaa acgcgccgac cagcgcggca atcagcaata aggctttaaa
    27481 aaaacgtcgg cttttcattt tcacttatcc tccaatgcct cgaccacttc ttccatctgc
    27541 ataaagcgcg aacctttcac caacacggtg gcgcgttcgg gcaaatcgtg gctcaacact
    27601 tgaatcaacg ggtctttggc ggcgaaccac aaaccgtccg cgccaaattt ttccgccgct
    27661 tcgacgctgt tgtcgccgac aaaataagcc gcttcgatgc cttggtcgcg ggcgtacgcg
    27721 ccgacttcgg cgtgcatggc ggcggcttcg tcctcgccca gttcgcccat atcgcccatc
    27781 acgaaaatgc gcggcgcagg catacgcgcc aacacgtcaa ccgcggcttt catactgtcg
    27841 ggattcgcat tataagtatc gtcaatcagg gttgcgccct tgatgccggc tttgacgttc
    27901 agacggcctt tgatgttgct gaagccttgc aaaccttccg ccacatcgtt caaactcaaa
    27961 ccggcagcca aagccagcgc ggcggcagcg gcggcgttgt ggacattgtg gcggccggga
    28021 acaggcagca ccacggcagt gcgctcgtcg ccgcacacca aatcaaattc gcacgacaaa
    28081 ggtttcagca cgatattttc cgcgcggaca tcgccgctat cgacgccgaa agtgcacgta
    28141 ttcaaattaa acgttgccgt tttgaagaca gccatattgg catcttcttg aggaatcagt
    28201 gccatgccgt ctgaacataa gcctgcataa atctcgcttt tcgctttggc aatatcgccc
    28261 actccgtcga aaccgcatcc gacatgggcg cgcagggcgt tgttgaccaa agcggcatcg
    28321 ggtttggcga tttgcgttaa aaccgccagt tcgccaaaat ggttcatgcc catttcaatc
    28381 acggcatagc ggtgtttttc gtttaatttc aataaagtca gcggcaatcc gatgtggttg
    28441 ttgaagttgc ctgccgtcgc cgaaacggca tcatcgccga aacggcggcg caataccgca
    28501 gccagcatct ccttcaccgt cgtcttgccg cccgaaccgg taatgccgaa cacaaacggg
    28561 ttcacattat cgcgccacgc cttcgccaac gtttgcaacg cggcaagcgt gtcatcgact
    28621 ttcaacgcgc cgcccaaagc cgcgcaatct tcgcgcgaaa ccacaaccgc cgccgcgccc
    28681 gcagacaata cgcctccaac aaaatcatgc gcgtcaaacc gcccgcccgc caatgcgaaa
    28741 aacacatcgc cttcccgaat atcgcggctg tcggttacga tgcgcgacac gggtttgttt
    28801 tcagacggca tcggaagctt gagggcttgg cagatgaaat ttaggtccag tggtttcata
    28861 tttgctttcg ttaatattcg ggcggcggac acatcggtag cggctgattt ttttatcgcc
    28921 tgttttgctg tggtaaaaca cagattattt tcccattctc attcggcatt ttttctgtac
    28981 gtatcatttt ttagacgtat ttttagccga tttgcctttt cccgcatacc acggcgcggg
    29041 gtcgtcggac tgtctgtcga taaaggcaag gttattgcct tcgcccggca catcggggac
    29101 attcccccaa aaatcatagc cgtcatcggg caactcgtcg gtttcgatac ccgtccaact
    29161 gccgaatccg cgtaaaaaat taaacgcgct gcgcctcact ttaatcattt cgcgccgcgt
    29221 gtcatcggac gaaattttaa ccagcggcac gccgtagctt tgacgcttcc actcgccgtg
    29281 tgtcaacgtt cgctcgccgc ctttgccgac atgcatcaaa ccgtggtcgg aaaagtaaac
    29341 caaagaccag ctttctttat tttcattcaa tatcttaacc gtatcttcta aaaatttatc
    29401 ggtttgcgcg atggtggaaa catagcagga tattttttca gtttgatact gaaaccgccg
    29461 cgcatcctta tccaaacgtg tgcaaaaatc actgtgcgaa cccatcaggt gcatcacaat
    29521 cagccgaggc ttcgtgcctg catttcctgt caaaacccgt ttgaacgccg gcaacaaaag
    29581 gcggtcgctc aatcccgcgc ttttgccata atcacccctt tgggtaaacc acggataatc
    29641 gctgcgtagg gcataggtgg aaatttcgtt ggcaaaatgc cccaacattc cttgattaga
    29701 cagccacgcc gtccgaaaac ccgcctgctt cgccaagctg acaatgttat tgttcggctc
    29761 tcccggcagc cccaaagtct gcggcagcga aagattcgtc gcgtgggcgg tcgattggta
    29821 accgtttatc aacagccctt tggtccgact caaaaaaggc gtatcgggca atgggaaacc
    29881 gtaaacattc atataatccg aacgcgcgct ctcaccgata accacaacat aatttttata
    29941 cttgggcgca acatgacgga tatgccatgt tgacgctttt tttgctgctt ccaaaatgtg
    30001 ggcgcgcttg gcggcatatt gtgccggcgc ggaagccaaa tcgtaataca ggtcgaaaat
    30061 attcaacaac aggccggcat caggttctcg ccaatcttta tcgccggcga ttttctccat
    30121 caccgcgcag gataaaatca aagtcaataa tatggtcagc catattttgc tgcggcgttt
    30181 atagtttttt acgtcagcaa atacccccac agatacacaa tatttccaaa ctgtcaagcc
    30241 aaaaatgaaa aataatgcct gcacaaaata aagcgacccg ggaagattgc cgacaaattc
    30301 acgcgcctcg gcaggattgc tttccaatat cgaaccgact atctgataag aaggcgcacc
    30361 atacagccag ccgaccggca aatatagggc ggttgtgccg acgtaaatca gtaaaacaac
    30421 tgaagaaacg cgcgggaagc cgcgtgccaa taataataaa aatacaaccg aagccaaagc
    30481 gcccacggca gaataacggt agccgtattc atattccaag tgataacctg atgcgatggc
    30541 cgcgccaaaa caaaaggcta aaagcgcgca aatgcccaat gtatttttta atgtctgatc
    30601 catatttttt attcgggcgg attgtttcat acgatgccgt ccgaatatcc cattatttac
    30661 gagttaacaa agcctgcccg acgatttcaa gatcggaaaa acggtgcttc acgccctgta
    30721 catcctgata gttttcgtgt cctttgccgg caatcaggat gatgtcgttt gcggcggcct
    30781 gctcaaccgc ataacggatg gcggcggcac ggtcggcttc gacgcattcg ggcgcgggca
    30841 cggcgggcag gatgtcgttg atgatgtctt gcggattttc caaacgcggg ttgtcgctgg
    30901 ttacgacgac tttatccgcg ccctgtacgg ctgccgcgcc catcaatggg cgtttgccgc
    30961 aatcgcggtt gccgccgcaa ccgaatacgc accataaggc cgcaccctgc ggtttgattt
    31021 cctgcaaagt ggagagtgct ttttccaatg cgtcgggcgt gtgggcgtaa tcgacgacga
    31081 ccaagggctt gccgctgttc atgatgcagt ccatgcgccc tgaagcggga cggatttttg
    31141 ccagcacatc caatacctta tcaagcggat aaccgttggc acaaagcaag gcgatgcagg
    31201 cggcgaggtt ttgcgcgttg aaccgtccga gcaggcgggt gcggcatttt ccttcgcccc
    31261 acggggtttg gaatacggct tccatgccgt ctgaagaggc ggtaaagtcg gtaatgcgga
    31321 tgtcggcgtg ttcgctgaaa ccatagctgt aaacggctaa atcgggacag tcttttttca
    31381 gacgacctat gagttctgcg ccgtattcgt catctacatt gatgatagcg tgcttcaagc
    31441 catgccagta aaacagtcgc gacttgatgg caccgtaggc ttccatcgtg ccgtgatagt
    31501 cgaggtggtc tcgggtgagg ttggtaaaga ttgcgctgcg gaatggcacg ccgttgacgc
    31561 gcgactggtc aagaccgtgg ctggagactt ccatcgcggc ggctgttgcg ccctgttggc
    31621 ggaaacggta gagcagggtt tggacatcga cgggggcggg ggtggtgtgc gtggtttctt
    31681 ccaatgcgcc ccaaaagccg ttgccgaccg tgccgataat ggcggttttt tcgcccaata
    31741 aatcggcagc ctgcgccagc cattgtgtga tggaggtttt gccgttggtg ccggttacgc
    31801 cccagacttt gaggccgtct gaaacgttgt cgtacacttg cgccgccaac atgccggcac
    31861 ggtgtttcaa atttttgatg ccttgattgg ggactttcca ttcgggattc cacgcgaatt
    31921 tgccgtcgtc gtcccaaaaa acaaaagccg cgccgttgac aacggcggcg gggatataac
    31981 tgcggccgtc ggtatattcg ccctgacagg caacgaaaat atcaccttgt ttgatttgac
    32041 ggctgtctga atgcaacaaa cgccctgctg cgtttgcaca cagcagaggc ggaatgttgg
    32101 tttcagctaa aggggttaac ttgctgaaca taaaataatc tcgttgatac tcggattaag
    32161 acggtgtttt gacggctgca acattggtca aaggtttggt cggggaaacg cccaagatgt
    32221 tcaggctgcc gcccatgact tgtttgaaga ccggacctgc cactacgccg ccgtagtaac
    32281 cgtttgcagt cggctcgtcg atggtaacgg ccacaatcac gcgtggattt ttagccggag
    32341 caaaaccgat aaacgtaccg acgtgtttgt tgtccacata gcggccattg accagtttgc
    32401 gcaccgtacc ggttttagcg ccgacatcga aaccgtccac cgcacccgcc gtaccggtgc
    32461 cgcccggctc ggttacggaa accatcagat tgcgtacctc gcgcgcggtc gattctttga
    32521 atatgcgttt gccttgcggc gcaaccgcct gcttctcaaa gctgagcggc agcaaaacgc
    32581 cgtcgtgcgt cagtgcggta taggcgcgcg ccaattgcag caggctcaat tgcaggccgt
    32641 aaccgaaaga catcgtcgcc tgttcgatgg gccgccacct gcgccaattt ctcaacaaac
    32701 ctgcagtttc ccccggaaag cccgagtgca tacgcacacc gatgcccaat tcatgataga
    32761 agtcatacat ttcttcggcg ccgaaacgcg cagacagttt gcttgtgccg acgttggacg
    32821 atttctgcat aatgccgcgc acatccaaag aggggtaaac atgggtatcg tcgcgcacgg
    32881 gagacggtcc gattttataa ggctgcgtat tcagccgttc gttcaaatcg gttttgcccg
    32941 catccaatgc cttcgcaatc acgaacggtt tgattgccga accaggttcg atcatatcgg
    33001 ttacggcacg gttgcgccgc tgttcgctgt ctgcccggcc gggtctgttg ggatcgtagg
    33061 cgggcgtatt ggccaaggcg aggatttccc ccgtgcgggc atccaaaacc accaccgttc
    33121 cggcttttgc ctgatggtat tcgaccgcct tgttcaactc ttcataggcc aaggtctgaa
    33181 tcctctgatc gagggaaagg atgatgtctt tgccgttttg cggtgcttta ttgcgcgggg
    33241 agtccaagct gtccacaata ttgccctgcc ggtcccgcaa aacaacttcc gcgccgtctt
    33301 cgccatacag gctgtcttca agcgaaagtt ccaaaccttc ctgacctttg ccgtcaatat
    33361 cggtaaatcc gatgacgtgt gcaaacaggt tgcccatcgg gtaatggcgt tttaattctt
    33421 tttcaaatac aaagttttcc aaacccaagg ctttgacctc ttcggcaacc ttgggatcga
    33481 gctgccgctt gatccaaata aacgacttgc ctttctgttc gagtttgttc ctcaaaacat
    33541 cgaccggcac atcgacaagc tcggacaggc gttccaattg ggcggcagac ggcatttcct
    33601 tcatatcttt aggcacggca aacagggact ccgtcggcgc gctcaacgcc aaaaccgcac
    33661 cgttccggtc cgaaaccgta ccgcgtgtag ccggcaatgc ttgagtccgc acaatccggt
    33721 tgtcgccctg ttctttcaaa aagttatacg ttaccgtctg cagatacagc ccgcgggcaa
    33781 tcagacaggc aaacaagacc gccattgcca tcaggacgaa gctaatccgt ccgttactgg
    33841 tcatcggctt tttgacctgc tcttctttgg gcagcatccg gggcttatat tcgcttttaa
    33901 tcaacatttt tacttctcgt tattattatc ctgatgcagg aatccgattc cggcacacag
    33961 gctgcttcta tctttgatgt tccaccataa aggtattgcc cgaaaccggc ggatggaggt
    34021 tttgtttttc tgccgccgcc ctgatcgctt cgtgattcgc caaacgcgcc tgttgcaccc
    34081 tcatttgcgc ataatcctgc tccaaggcga tttcctgttt tttcgccttg tccaaagctg
    34141 tgaagttgag cctgtactgg ttttgctgca tcacaacgga aaaagcggaa acgcacaccg
    34201 caagcagcag aaagaaattc gatttgttca ttgccgttca gacgtttttc tctgttatta
    34261 ttccggtatc ggaccggcag tccgctccgc cacacgcaaa actgcgctcc tcgccctcgg
    34321 gttggcggca atttccgctt cacccggctt taatgccctg cccacgattt tcaggggcgg
    34381 ctcgggcaaa tccgcttctc tgaccgccgc ccagctcggc aggggctcgt gttgcgaata
    34441 ttttttgaca aactgcttca caatgcggtc ttccaacgaa tggaaagcaa tgaccgccaa
    34501 acgcccgccc tctttcagac ggcacatgac ctgcggcaat accgccccta cttcttcaag
    34561 ctcgcggtta ataaagatgc ggattgcctg gaaggtgcgc gtcgcaggat cctgcccccg
    34621 ctcgcgagta cggacgtttt gtgccacgat ctgcgccagc ttgcgggttg tatcgattgg
    34681 actttccgcc cgttgcgcga caatggcgcg cacaatctgg cggctaaacc gctcttcacc
    34741 ataattcttg attacctcgt gcaaatcctg ttccgacgca accgctatcc actctgcggc
    34801 agacataccg cgcgtcgtat ccatacgcat atccaaaggg gcatcgaaac ggaagctgaa
    34861 accgcggctg ccgtcatcga tttgcggaga cgaaatcccc aaatcaaaca gcgcaccgtc
    34921 caccttgccg atacccaaac cgtccaatgc cgtctgaaac gaagcaaaac cgccatgcac
    34981 gacaccgacc cgtttgtccg aacgcgccag ctcttctgcc acagcaatcg cctgcgggtc
    35041 tttgtcgaaa acaatcaacc gccccgcatc gcccaaacgc gacaaaatca gccgggaatg
    35101 ccctcccctg ccgaacgtac cgtccacata gacaccgtct tcgcgcacgg caagcgcatc
    35161 caccgcctca ttcagcaaga ccgtgatatg ccggtaactt tctgctccac tcacaattgc
    35221 aaatccgtct gactcaactg gaaggcaagt tcgtcaggat cgtcatccaa agcctgaacc
    35281 atctcagcct cccactgctc gcgaccccaa agctccaaac ggttggcacg gccgaccaaa
    35341 acaacttcac ggtcgaaatc cacccttttc ctcagtccgg caggaaccag caccctgccg
    35401 gcgctgtccc attccaaaat ttccgcgtta tgcagcaaaa gattttgaaa ccgccgcaaa
    35461 acagggttat ccgccacttt taagtttaaa agttgcgccg caaccttttc ccactccgca
    35521 acagggtaca tcaacagctt atgtttcgac tcgagcgttg ccactacggc aggcgtatag
    35581 aggcgcgaca gaatgtcgcg gaatttggca ggaacagcca accgcccctt actgtcgatg
    35641 cttaattcgt gtgcgccgcc gaacatgaca tgtcccaagc cgaaatcaaa atcacaaggg
    35701 taaaagagac actttgcccc acaattcccc accaatcgac actataagaa attttaaaca
    35761 ctcggtcaaa tcagggcatg aaaactcatt aacatatctg aaatttttat tctgtttaaa
    35821 aacaataaga taaaaaatga cgacaacggc acggcgggtg cagtacagag tgaccgaacc
    35881 aataaacaac tatatatgat taatttaata atataaacac aatatatagt attaagataa
    35941 agccatgaca gcacccgtac caacgtgtaa tatgtcggga aatccaataa atttacacaa
    36001 gctaacattt atcatgctcc tcccctcccc cgaagcacgg caatcctcgc tcaatctgca
    36061 aaccctcatt gccgaagaaa tcggcaaaca cggcaattgg attccatttt cacgttttat
    36121 ggaattggtt ttatacgctc cgcaatacgg ctactacacc ggcggcagcc ataaaatcgg
    36181 caataccggg gattttatta ccgcaccgac cctcacccct ctgtttgcac agacactggc
    36241 acgccaactt caagaactcc tgccccaaac ggcgggcaat atctatgaat tcggcgcggg
    36301 aaccggacag cttgccgccg atttgttggg cagcgtttca gacagcatca actgttacta
    36361 tattattgaa atatcgccgg agctggcagc acgtcagaaa aacctgattc aagcccgtgc
    36421 accggaagca tctcaaaaag ttgtccactt gaccgcactt cccgaagcgt ttgacggcat
    36481 catcatcggc aacgaattac tcgatgccat acctgtcgaa atcgtccgta aaaatgaagg
    36541 aggattactc gagcacatcg gcgtttgcac ggataacggc cgtttcgcct attctgcaag
    36601 acccttgcac gacccgtcgc tttccacctc ggcttccctc tattttcctc aaacagatta
    36661 tccctatacc agcgaactgc acccgcaaca atatgccttt atccgcaccc ttgcctcaag
    36721 gcttgaacgc gggggtatga tattcatcga ctacggtttt gatgcggcgc agtattacca
    36781 cccgcaacgc aatcaaggca cattgatcgg acactaccga caccacgtta tccacaatcc
    36841 tttcgacttc atcgggttgg cagatctgac cgcccacgtc aactttaccg acattgccca
    36901 agccggtacg gatgcaggac tggatttgac aggctatctg ccccagtccc attttctgct
    36961 gaatctgggc ataaccgaac tgttggcaca gacgggtaaa acagattcgg cagcctatat
    37021 ccgtgaagcc gccgctgttc aaaaactgat tgaccagcat gaaatgggcg aactgtttaa
    37081 agtcatcgca ttcggaaaaa atatcggcat cgactgggca ggattccgct tcggcgacat
    37141 ctgccacaaa ctccaaccct cgtgccgtct gaatccgctt cagacggcat aaacttttta
    37201 acatttaaaa acaagaaact aattcaaaat taaaaaatac ggcttgtcaa aaaaacaaaa
    37261 aaacatataa tagctaattc acgaaacggc gaattagctc agtcggttag agcagaggaa
    37321 tcataatcct tgtgtccggg gttcgagtcc ctgattcgct accagatttt cgggggtata
    37381 gctcagttgg tagagcgctt gcatggcatg caagaggtca gcggttcgat cccgcttacc
    37441 tccaccagat aaaaaagcac agaccgtaaa aggtatgtgc ttttttattg cctgattgcc
    37501 ggaaaataaa gaataaacca ctgccttcaa aacaggcaat cgactttaaa cctattgccc
    37561 cgcctgtcct gattttatca gcccgccaga caaacccgac ctgaataatg tcttcaggtc
    37621 gggtttatgg tttcattccc aacttatcca gcccgacaac cacaatatta tgatgacaat
    37681 gccgaaaaca atgcggtaat aggcaaacgg gatatagttt ttcttggaaa caaacttcag
    37741 cagtgcttta accgctacca aaccggaaac aaaagcggca ataaagccta tcaaaatcaa
    37801 accgacatca tgcagggtga aaaatcggta atgtttcagg acatcataag ccgttgctgc
    37861 aaccatcatc ggaacggcca agaaaaatga aaactccgtt gccgttttcc gctcgattcc
    37921 ccaaaacatc ccgcccataa ccgtactgcc cgaacgggac gtacccggaa ccagtgcaaa
    37981 cacttgggca acaccgatca tcaacgcatc aatcggacgc aatgcatcaa catcggcaat
    38041 tttaggctct gctcggcttt ggcgtttctc cacccacaaa ataaaaaaac cgcccaaaac
    38101 cagcatgact gcaacactca aggggttaaa cagatactct ttgatttgtt tgtcgaacaa
    38161 cagccccatc acggcggcag gtataaaagc aatggcaaga ttgaggacga aacggttggc
    38221 tttccggtct tttcccacgc catgcaacac attgctgaaa cgctgccggt attcaaacac
    38281 taccgccaaa accgcaccga gctggatggc aatttcaaaa accttgtgat tgctgtgaaa
    38341 accaatcaga ttgccgaaca caatcaaatg tccggtgctg gaaatcggta aaaattcggt
    38401 aaaaccttct accaagccca tcatcagggc tttcaggaca atcagaaaat ccattgcttg
    38461 cgcttctttc ggatacggga gttcggctat ttctgtacag caggggtctg acgcttgcgt
    38521 tcttccctga ctttggcaac caattcttta atcgtatgaa cgccgccatc aaagccatta
    38581 ttgaagataa cgcggtattt tccgccgaca ataacggtcg gcgtgccgtc aataccgtat
    38641 tgttccgtca gtttctgcat ttttaatgcg acggcggcag cttcggggga atcataggcg
    38701 cgcatcagtt ttttgccgtc aaaacctttt tgagataaag cccattttcc ggcaacagcc
    38761 ctgttttcca aacggatttt ttgttcgtaa actgctttaa acacagcaga gtttgcctga
    38821 tatttcaaac ccgacagctt gaccgcagca gccattcttg ccagaccgag catttcaggc
    38881 cgccagacca cgtgctccgt ccgcagataa gtatcagacg gcaatgcctt gcccagtttc
    38941 aataacaaag gatcgaaatg atggcaatgt acgcaaaaat agccgaaaaa ttccaaaacc
    39001 tcaatttttc ccggctgttc ttgaggaatg ggtttatcca acacaagata gtcttcccct
    39061 tccgtcaggg catatgcctg cgcggacaac actgccgaca gcagcagcgg caacagatgt
    39121 ttaaacttca taattatttg ccttcaatcg cacggatcag gctggcaacc ccatgctttt
    39181 tcaactcgtc ctgcattttt ttcaccgcat cggcagacat attgccgctt tgcacgcggt
    39241 aaagcgtttt atgtcccgcc tgatagccga ccacttcgga agatatgccc aagattgcca
    39301 gtttggcacg ctgcccttcc gcgctccggc ggtcggcata cgcgcccatt tgcagataat
    39361 gcgttgcttc cgccttgcca aacgttttca ttttctgcac ttctttggca gcggcactgc
    39421 gcgctttttc gatgctgccg ctgttgagga tttgttccgg ggtcggtttg ggtgcaactt
    39481 tttccttcgc cgcctttttc tcttcttttg aagctttttt ctctgtttct ttagacggtt
    39541 ttaccgcttg ttttttaacc gtttcggcat ctttcttccg cgctttttcc ctgacggttt
    39601 gttcacgctc ttccgtcagt gctttcttgc gcactgcctg tccgtccggc tcttcccgtt
    39661 ccggctcgcc cgccttttct tcaacctcgt cggctttgtc ggcaacgggc tgcttgtcgg
    39721 cagctttttc cgcatccgac tgctctgcct ctttcgcaac atccggttcg gacaaggcgt
    39781 tttgatcggc cggttcaggt tggatgtctt ccttaggctg gtttttcagt ttcaggattt
    39841 ccgtttctgc aggctgcttc gacggagccg ggattttgaa cgcattttga ccgccctggt
    39901 tcagataaag caaaataccg gcaataatga ccgttgccag tatcaaaccg aagaagaaac
    39961 cggacagacc ttttccggat tgggaaaatt tgttcataaa cataccttaa tgtgtttcag
    40021 acggcattaa cgccgtttgc ttacgggcgg atattctaac aatatcgcca tatttgggca
    40081 aaacctgctt ccattcccgt tcctataaag cacgacggaa gcctatggct ttcatgacgt
    40141 ggcttctgcc gacttcttca tcgcccgcca aatccgccaa tgtacgcgcc acgcgcataa
    40201 tgcggtggaa gctgcgggcg gaaagagaca atttttccag caggctaccc aaggcttcct
    40261 gcgcttcttt ttggatgcag gcttgggaat cgagttcgct gacgctcaaa gcggcgttga
    40321 ctttgccctg ccgtgcgtat tgtttgccgc gggcggcgat gacgcgttcc aaaacggacg
    40381 cactgctttc gcccgcttcc tgctgcatca gttcggcggc ggacaggctg gggacttcga
    40441 tggtcaaatc gatgcggtcg agcagcggac cggaaatttt gcttcggtaa cgcgcgacac
    40501 tttcgggcgt gcagcggcag ggtttgacgg gatgcccgag ataaccgcac ggacacgggt
    40561 tcatggcagc gacaagctgg aatttggcgg gatagacggc ttggcgcgcc gcgcgggaaa
    40621 tgtggatttc gccgttttcc aacggttcgc gcaaaacctc caaaactttg cggtcgaact
    40681 cgggcaattc gtccaaaaat aaaacgccgt gatgcgccaa tgaaatctcg cccggacgcg
    40741 gatccgagcc gccgccgacc atagccgccg cgctggcgct gtgatgcgga ctgcggaaag
    40801 gacggttgct gtcgagttgt tgttggtggt tgggcaggag cgaacgcaat gcccaaactt
    40861 ctaccaattc gtcttcggcc agcggcggca ggatgccggg cagccgttgg gagagcatag
    40921 acttgcctgt tcccggcggg cccatcatca agaggctgtg tccgcctgcg gcggcgattt
    40981 ccaaagcaag gcgggcagag tgctgacctt tcacatcaca caaatcaggt tgtccgccat
    41041 gttcagacgg catttgcggt acggaacatt cggtttgcgc caaaggttcg ataccgttca
    41101 aatgagcggc gacttcgccc aaagagcgcg cgccgtaaac ggtaatgccg cgcatcacgg
    41161 cggcttgtcc tgcgttttct tcaggcaaaa caaatgcacg ttttgcctgc ataccctgcc
    41221 acgccatcgc caacgcgccg cgcacggggc gcaacaaccc cgacagtgcc aattcccccg
    41281 caaactcgta ttccgccagc ttttcgggtg caacctgccc cgatgcggca aggatgccga
    41341 ttgcaatcgg caaatcgaaa cgccccgact ctttgggcag gtcggcaggg gcgaggttga
    41401 cggtgatttt tttggcgggg aattcaaaac cgctttgaat aatggcggca cggacgcggt
    41461 cgcggctttc ctttacttcc gtatcgggca gtccgacgat gttgaaatgc ggcaggccgt
    41521 tggcaaggtg ggcttccact tcgaccaacg gcgcattcat accgctcaag gcgcggctgt
    41581 aaaccaaggc aagcgacata tttcagacgg ccttattcgc cggcttcggt ttgctgcctg
    41641 atttcggcga cggcttcttc ggcagcggct tcagccgctt ccaatgctgc ccgttcggga
    41701 ttttgcgcgg cttcgagttt cgccagacgt gcttccaaag ccgccagttt ggtacgggtt
    41761 ttaatcaaaa cctgctgctg gatgtcgaat tcttcgcgcg taaccaaatc catacggttg
    41821 aacgcgccgc ccagcatcgc cttaatattt ttttccacat ctttggcagg gctgttggcg
    41881 atggtttcgc tgattttcga gccgacttct tcaaaaagct gcttgccgaa cataatctgc
    41941 atccttcctg aacatatcaa attcaagcgg ctattgtata aggaaaaatg ccgtctgaaa
    42001 acgggcggcg gataatcgtc aaaacatacc gcgccccctt tgcggttgct aaaattacac
    42061 ttcgagacac ctgccgcctg aaaacggatt tcatattccg cgcaacgccc gattacatta
    42121 caagacacta caaaacaata tgctgtttta aatgattttt ccgatgcgca tcgtttcaaa
    42181 ctcggctttt taagccatta agtgctttgc aaacaacaga aattgggtta tgctgaaacg
    42241 gattatttac aatttcatat agtttcatta catatcttat tgtaattgaa gataatttat
    42301 ccgaatcccc tttcgggtat ccggattttc cgttgtactt tcattagaaa aacatttcag
    42361 gcgcaagttg cttgcaattt caaagcctgt aattctgaag ttttagacga ggaatagcac
    42421 atgaatccca tgtacatcac ttttgcaatc tatttggttg cagtccttct catcgggctt
    42481 gccgcttatt tttccacgcg caatttcgat gattatattt tgggcgggcg cagcctgggc
    42541 ccgtttgtta cggcgatgtc ggcaggcgcg tccgatatgt ccggctggct tttgatgggt
    42601 ctgccgggcg cgatttattt gagcggtttg aatgaggctt ggattgccat cggcctcttg
    42661 gtcggcgcgt atttcaactg gctcttggtg gcgggccgcc tgcgcgtgca taccgaatat
    42721 gccaacaatg cgctgacgct gccggattat ttcttccacc gcttcggcgc gggcggacac
    42781 ttgatgaaag tggtttccgc actgattatc ctgtttttct tcacgattta ttgcgcctcg
    42841 ggcattgtgg cgggcgcaac cctgttccaa agcctgtttg aaggtatgac ttacaatcag
    42901 gcaatgtggc tgggcgcggg cgcgaccatc gcctatacct tcttgggcgg cttcttggcg
    42961 gtcagctgga cggatacgct gcaggcttct ttgatgattt tcgccctgat tttaacgccg
    43021 gtgatggtct atctgggctt gggcggcgcg gaacagatgt ctgccgcgat tcaaagcgtc
    43081 gccgcaggca cgggcaaaga atacggcagc ctgtttgccg gtacgaccgt catcggcatc
    43141 atttccaccg ccgcctgggg cttgggctat ttcggacagc cgcacatttt ggcgcgcttt
    43201 atggccgccg aaagcgcgaa atcgctggta tccgcacgcc gcatcggcat gacttggatg
    43261 gcgttgtgcc ttgcgggcgc ggtagcggtc ggttatttcg gcattgcgta tttcggtgca
    43321 aaccccgaca aagtttcttc tatgagcggc aaccacgaac gcatcttcat cgcgctttcc
    43381 accctgctgt tcaacccttg gatcgccggc atcattttga gcgcgatttt ggcggcggtg
    43441 atgtccaccc tgtcctgcca gcttttggtt tgctccagcg cgattaccga agacttctac
    43501 aaaggctttt tgcgtaaaaa cgcgcaacag tcggaattgg tatgggtcgg ccgcctgatg
    43561 gtattggcga ttgccgtgat ttccatcctg attgcttccg atccgaacag caaagtattg
    43621 ggactggtgt cttacgcgtg ggcaggtttc ggtgcggcat tcggcccgat tgtgattctg
    43681 tccgtattgt ggaaacgcat cactgcctac ggcgcgttgt caggcatggt tgcaggtgcg
    43741 tcaaccgttg ttgtgtgggc ggaatgggta aaaaaaccgg ctcgagcggc aggcgaaagc
    43801 ggcttgttga cgatgtacga aatcgtcccg ggctttattg tttgcttgat cgttgccgtg
    43861 ttggtttccc tgttcaacaa agaaccttcg cgcgaaatcc aagaacgctt tgaaaaagcc
    43921 gacgcagatt accgcgccgc ccgataaacc cctttcagac ggcacggcat cccggttcgt
    43981 gccgtctgaa cccgcttccg ggccaaacac aaataaacct tgcctgcata caggccttat
    44041 ttgtgtttta cccgtttcat tttcaaagaa acggaagcac cgattctgcc cgcatttgaa
    44101 gccttgtatt tcatctgcgg atttattacg atattaccgt attcaggccg caccgatgcc
    44161 gtctgaaccc cccgaaaaaa ctttggagga tccaaaaaat gtttcatttt gcatttccgg
    44221 cacaaactgc cctgcgccaa gcgataaccg atgcctaccg ccgtaatgaa atcgaagccg
    44281 tacaggatat gttgcaacgt gcacagatga gtgacgaaga gcgcaacgcc gcctccgagc
    44341 ttgcccgccg tttggttacc caagtccgcg ccggccgcac caaagccggc ggcgtggatg
    44401 cgctgatgca cgagttttca ctctccagcg aagaaggcat cgcgctgatg tgtctggcag
    44461 aagccctgct gcgtatcccc gacaacgcca cgcgcgaccg cctgattgcc gacaagattt
    44521 cagacggcaa ctggaaaagc catttgaaca acagcccttc cctcttcgtc aatgctgccg
    44581 cctggggtct gctgattacc ggcaagctga ccgccacaaa cgacaaacaa atgagttccg
    44641 cactcggccg cctgatcggc aaaggcggcg caccgctcat ccgccaaggc gtaaattacg
    44701 ccatgcggct tctgggcaaa cagttcgtca ccggacagac cattgaagaa gccctgcaaa
    44761 acggcaaaga acgcgaaaaa atgggctacc gcttctcctt cgatatgttg ggcgaagccg
    44821 cctacaccca agccgatgcc gaccgctact accgcgacta tgtcgaagcc atccacgcca
    44881 tcggcaaaga tgcggcagga caaggcgttt acgaaggcaa cggcatttcc gtcaaactct
    44941 ccgccatcca tccgcgctac tcgcgcgccc aacacggccg cgtgatgggc gaactgttgc
    45001 ctcgcctgaa agaattgttc cttttgggta aaaaatacga tatcggtatc aacatcgatg
    45061 ccgaagaagc caaccgtctg gagctgtctt tggatttgat ggaggcactc gtttccgacc
    45121 ccgatttggc cggctacaaa ggcatcggtt tcgttgttca agcctaccaa aaacgctgcc
    45181 cgttcgtcat cgactatctg attgacctgg cccgccgcaa caaccaaaaa ctgatgatcc
    45241 gcctcgtcaa aggcgcgtat tgggacagcg aaatcaaatg ggcgcaagtg gacggcttga
    45301 acggctatcc gacctacacc cgcaaagtgc ataccgacat ctcctacctc gcctgcgcgc
    45361 gcaaactgct ttccgcgcaa gacgcggtat tcccgcaatt cgccacccac aacgcctaca
    45421 ccttgggcgc aatctaccaa atgggcaaag gcaaagattt tgaacaccaa tgcctgcacg
    45481 gtatgggcga aactttgtac gaccaagtcg tcggtccgca aaacttaggc cgccgcgtgc
    45541 gcgtgtacgc cccggtcggc acacacgaaa ccctgctcgc ctacttggtg cgccgcctgt
    45601 tggaaaacgg cgcgaactcg tctttcgtca accaaatcgt tgatgaaaac atcagcatcg
    45661 accgcctgat taagagcccg ttcgacacca tcgccgaaca aggcatccac ctgcacaacg
    45721 ccctgccgct gccgcgcgat ttgtacggca aatgccgtct gaactcgcaa ggcgtggact
    45781 tgagcaacga aaacgtattg cagcagcttc aagaacagat gaacaaagcc gccgcacaag
    45841 acttccacgc cgcatccatc gtcaacggca aagcccgcga tgtcggcgaa gcgcaaccga
    45901 ttaaaaaccc tgccgaccac ggcgatgttg tcggtacggt cagctttgcc gatgccgcgc
    45961 ttgcccaaga agcggttggt gcagccgttg ccgcgttccc cgaatggagt gcgacacctg
    46021 ccgccgaacg cgccgcctgc ctgcgccgtt tcgccgacct gctcgaacag cacacccccg
    46081 cgctgatgat gcttgccgtg cgcgaagcgg gcaaaacgct gaacaacgcc attgccgaag
    46141 tgcgcgaagc cgtcgatttc tgccgctact acgcaaacga agccgaacac accctgcctc
    46201 aagacgcaaa agccgtcggc gcgattgtcg ccatcagccc gtggaacttc ccgctcgcca
    46261 tctttaccgg cgaagtcgtg tccgccctgg cggcaggcaa caccgtcatc gccaaacctg
    46321 ccgaacaaac cagcctgatt gccggttatg ccgtttccct catgcacgaa gccggcatcc
    46381 cgacttccgc cctgcaactc atcctcggcg caggcgacac gggtgcggca ttgaccaacg
    46441 atgccagcat cggcggcgtg attttcacag gctcgaccga agtggcgcgc ctgatcaaca
    46501 aagcccttgc caaacgcggc gacaatcccg tcctgattgc cgaaaccggc ggacaaaacg
    46561 ccatgattgt cgattccacc gcacttgccg aacaagtctg cgccgacgta ttgaactccg
    46621 ccttcgacag cgcgggacaa cgctgctccg ccctgcgtat cttatgcgtc caagaagacg
    46681 ttgccgaccg tatgctcgac atgatcaaag gcgcgatgga cgaactcgtc gtcggcaaac
    46741 cgattcagct cactaccgat gtcggccccg tcatcgatgc cgaagcgcag caaaacctgt
    46801 tgaaccacat caacaaaatg aaaggcgttg ccaagtctta ccacgaagtc aaagccgccg
    46861 ccgatgtcga ttccgaaaaa tccacgttcg ttcgccccat cctgtttgaa ttgaacaacc
    46921 tcaacgaact gcaacgcgaa gtcttcggcc ccgtcctgca cgtcgtccgc taccgcgccg
    46981 acgaactcga cagcgtcatc gaccaaatca acagcaaagg ctacgccctg acccacggcg
    47041 tacacagccg catcgaaggc acggtacgcc acatccgcag ccgcatcgaa gccggcaacg
    47101 tttacgtcaa ccgcaacatc gtcggtgcag tcgtcggcgt acagcccttc ggcggacacg
    47161 gtctgtccgg cacaggcccc aaagcaggag gttcgttcta cctgcaaaaa ctgacccgca
    47221 tccccgaatg ggttgccccg accctgagcc aaatcggaca ggcagacaaa gccgcgctca
    47281 aacgcctcga agccctgatt cacaaactgc cgttcaacgc cgaagagaaa aaagccgcag
    47341 cggccgcttt gggacacgcc cgcatccgca ccctgcgccg tgccgaaacc gtccttaccg
    47401 gcccgaccgg cgagcgcaac agcatctcat ggcacgcgcc aaaacgcgtt tggatacacg
    47461 gcggcagcac ggttcaagcc tttgccgcac tgaccggact tgccgcctcc ggcgtacagg
    47521 cagtggtcga acccgacagc ccgctggctt cctacaccgc cgacttggac ggtttgctgc
    47581 aaatcagcgg caaacccgaa aacaccggtg tgaaccacgt cgccgcccta agcccactgg
    47641 acagcagccg caagcaggaa ctggcaaacc gcgacggcgc actcatccgc atcctcccgt
    47701 ccggacaagg cttggacatc ctgcaagtgt ttgaagaaat ttcttgcagc atcaacacca
    47761 cagccgccgg cggcaacgcc ggcctgatgg cggttgccga ctgattttgc caaaccgccc
    47821 gggcgcggcc cgtgaacaaa tgccgtctga aaacctttca ggcggcattt tatagtggat
    47881 taacaaaaat caggacaagg cggcgagccg cagacagtac aaatagtaca gcaaggcggg
    47941 ccaacgctgt actggtttaa attcaattca ctatatgtgt tggcacatcg ctccaaacct
    48001 gatataatcc gcctttcaac atcagtgaaa atcgttcttt ttagtcagtt aacattaaat
    48061 ttcggagtcg aaaaatgaat ccagccccaa aaaacttctc ttctcttctc ttctcttctc
    48121 ttccgcagcg c
//
LOCUS       DAXDXW010000003        43631 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXDXW010000003 DAXDXW010000000
VERSION     DAXDXW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 43631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43631
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.003"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(109..1782)
                     /gene="recN"
                     /locus_tag="ACFB64_000114"
     CDS             complement(109..1782)
                     /gene="recN"
                     /locus_tag="ACFB64_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC1208575.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPALKAELREQGLLDEGGEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGGQLIDIHGQNDHHSLNQEAAQRELLDAFVGGRVQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGHSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAGRLSSETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(1966..2568)
                     /locus_tag="ACFB64_000115"
     CDS             complement(1966..2568)
                     /locus_tag="ACFB64_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC1208576.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLIEMPPTDEEEPETVPSDTLPN"
     gene            complement(2669..3610)
                     /gene="ylqF"
                     /locus_tag="ACFB64_000116"
     CDS             complement(2669..3610)
                     /gene="ylqF"
                     /locus_tag="ACFB64_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC1208577.1"
                     /translation="MAIQWFPGHMNKARKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDDNSWLEWIAKKRGAVLSGGRVNYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3691..4545)
                     /gene="rapZ"
                     /locus_tag="ACFB64_000117"
     CDS             complement(3691..4545)
                     /gene="rapZ"
                     /locus_tag="ACFB64_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC1208578.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDLGYFCVDNLPLEMLPSLVSYH
                     IERADETELAVSVDVRSGIDIAQAREQIAYLRGLGHRVEVLFVEAEEAVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVVLESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLAQEM
                     VDGIERFVTRWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSGR"
     gene            complement(4526..5488)
                     /gene="hprK"
                     /locus_tag="ACFB64_000118"
     CDS             complement(4526..5488)
                     /gene="hprK"
                     /locus_tag="ACFB64_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC1208579.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANDLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            complement(5492..5941)
                     /gene="ptsN"
                     /locus_tag="ACFB64_000119"
     CDS             complement(5492..5941)
                     /gene="ptsN"
                     /locus_tag="ACFB64_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC1208580.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHASVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVASAEEARAILTEE"
     gene            complement(6126..7016)
                     /gene="ubiA"
                     /locus_tag="ACFB64_000120"
     CDS             complement(6126..7016)
                     /gene="ubiA"
                     /locus_tag="ACFB64_000120"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC1208581.1"
                     /translation="MNLKSPLFLRLSDRLDVYIRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQFYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFAAIFAHTFFAK"
     gene            complement(7006..7413)
                     /locus_tag="ACFB64_000121"
     CDS             complement(7006..7413)
                     /locus_tag="ACFB64_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208582.1"
                     /translation="MNIIEIISRNRFLKQIYPSGIMDISLVSFSTDLSNCILTIRTST
                     KPFVEIEKWGLWLKDYDTVEIELRNSFIKGMKCQNWSHNNRNICQVEIKNQEDGLKII
                     RFYDNNSNWLLELEVYGLVFQGCKTYMKEGYES"
     gene            complement(7422..7517)
                     /locus_tag="ACFB64_000122"
     CDS             complement(7422..7517)
                     /locus_tag="ACFB64_000122"
                     /inference="COORDINATES: protein motif:HMM:NF026993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH/endonuclease VII fold putative polymorphic
                     toxin"
                     /protein_id="HFC1208583.1"
                     /translation="MRQPASKPHFNIRDSNLDTGHVDGTHGHYNF"
     gene            complement(7537..8013)
                     /locus_tag="ACFB64_000123"
     CDS             complement(7537..8013)
                     /locus_tag="ACFB64_000123"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC1208584.1"
                     /translation="MEHLFGKWLPDLPAPVSDGIDLPMSRLLKARSLTAALCALPHTF
                     SVELLKLGEAETEYGRRRVRDVLLKLDGTAVVQARSACSVGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSSEGCGRYFAARRSRFSHQGEEMLLTEYFLPELKRFIG"
     gene            complement(8028..8675)
                     /gene="bioD"
                     /locus_tag="ACFB64_000124"
     CDS             complement(8028..8675)
                     /gene="bioD"
                     /locus_tag="ACFB64_000124"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC1208585.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADEQRLTMPEIFSHPASPHLAARLDGRGLDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEKLLTIDHIQQQAYPVILVTSGRLGSINHTLLSFV
                     VLKQYGIRLHSLVFNHIHDSRDAHVAQDSLNYLQCRLKADFPEAEWMELAKTGAV"
     gene            complement(8672..9973)
                     /gene="bioA"
                     /locus_tag="ACFB64_000125"
     CDS             complement(8672..9973)
                     /gene="bioA"
                     /locus_tag="ACFB64_000125"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC1208586.1"
                     /translation="MPSEHQHTSSLLNFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVENQMKQMAHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKNRFDDEWDGADLQPVRALFEAHHVDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRAFGRLVYLMPPYIISDGILTKLADKTVQILKEHSK"
     gene            complement(10191..10415)
                     /locus_tag="ACFB64_000126"
     CDS             complement(10191..10415)
                     /locus_tag="ACFB64_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208587.1"
                     /translation="MSVWDIWDRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMPESPDFANAVAMAVAMICIVFIYD"
     gene            complement(10607..11092)
                     /locus_tag="ACFB64_000127"
     CDS             complement(10607..11092)
                     /locus_tag="ACFB64_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC1208588.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAVKSSGKVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFFHDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
     gene            complement(11268..11576)
                     /locus_tag="ACFB64_000128"
     CDS             complement(11268..11576)
                     /locus_tag="ACFB64_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208589.1"
                     /translation="MTNNVDLTIPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLKSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLEDLDKELC
                     "
     gene            complement(11560..11862)
                     /locus_tag="ACFB64_000129"
     CDS             complement(11560..11862)
                     /locus_tag="ACFB64_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC1208590.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKGMVEHYYDKQR"
     gene            complement(11936..14299)
                     /gene="pheT"
                     /locus_tag="ACFB64_000130"
     CDS             complement(11936..14299)
                     /gene="pheT"
                     /locus_tag="ACFB64_000130"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC1208591.1"
                     /translation="MQFSYSWLKTQANPDLSADKLEHLLTMAGLEVEEIDTAAPAFSG
                     VVVAEVKSVEKHPDADRLNVTQVDAGTGELVQIVCGAPNVKPGIKVPCSLPVAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVDGLHILPEDAPVGTNIREYLDLDDMLFT
                     LKITPNRADCLSVKGIAREVSALTQCAFTPVEIQTAPIGSEKKQAVRIDAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LIVRRAQNGETLACLNEKTVTLADNTLVVADEKGALSLAGLMGGAASAVSDGTQNIVL
                     EAAWFEPEIIVGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGKLPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRITAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMSELPETRRPRFAVYNEMAARGYRE
                     VISYAFVDEQWELDFAANAAPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIACVFGKGSDGRFVQNERIGGLWYGAAMPEQWGEKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEVMNHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGVATEAGARLRS"
     gene            complement(14390..15139)
                     /locus_tag="ACFB64_000131"
     CDS             complement(14390..15139)
                     /locus_tag="ACFB64_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC1208592.1"
                     /translation="MKKWTRDETLVALYLYYIIPFQKVSKDNPVIQEYAEILGRTPSA
                     LGMKIGNLGRLDPTLKVKNISGLSNGSKMDVVVWNEFSGDWEQLNKEFEGVISQYQSN
                     DENSNIEIESPEIPKGRERFARISVRVNQGFFRSSVLAAYNNQCCITGLKQPELLVAS
                     HIKPWGEDKDNRLNPRNGLCLNALHDKAFDRGLLGIDENFKIIFSPLLAKTEGFDDLF
                     KPYENRMIRLPERLNPSLEFLKFHRENIFQS"
     gene            complement(15145..15426)
                     /locus_tag="ACFB64_000132"
     CDS             complement(15145..15426)
                     /locus_tag="ACFB64_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208593.1"
                     /translation="MGLPYQNKDFRNMRLSDYILNNKININNLSDKQVQEIIDYFSSE
                     IDDWLLASEKELKDYDVYLYKLVLDGQQYVQGIRKSVLYDFLMENQKDS"
     gene            complement(15419..15637)
                     /locus_tag="ACFB64_000133"
                     /pseudo
     CDS             complement(15419..15637)
                     /locus_tag="ACFB64_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            complement(15744..16157)
                     /locus_tag="ACFB64_000134"
     CDS             complement(15744..16157)
                     /locus_tag="ACFB64_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC1208594.1"
                     /translation="MDKLTPEQRKKCMQSNKSTGTKPELVLAKAMWALGLRYRKNSGS
                     IFGKPDFSFKKYKVAVFVDGEFWHGKDWEQKKAVIKGNREFWIAKIERNIQRDIEVTG
                     RLKAEGWTVLRFWSNDVVKNTTCCAEKVKEIIQTR"
     gene            complement(16174..17163)
                     /gene="pheS"
                     /locus_tag="ACFB64_000135"
     CDS             complement(16174..17163)
                     /gene="pheS"
                     /locus_tag="ACFB64_000135"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC1208595.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFE"
     gene            complement(17404..17763)
                     /gene="rplT"
                     /locus_tag="ACFB64_000136"
     CDS             complement(17404..17763)
                     /gene="rplT"
                     /locus_tag="ACFB64_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC1208596.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(17776..17973)
                     /gene="rpmI"
                     /locus_tag="ACFB64_000137"
     CDS             complement(17776..17973)
                     /gene="rpmI"
                     /locus_tag="ACFB64_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC1208597.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(18120..18641)
                     /gene="infC"
                     /locus_tag="ACFB64_000138"
     CDS             complement(18120..18641)
                     /gene="infC"
                     /locus_tag="ACFB64_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC1208598.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(18659..20572)
                     /gene="thrS"
                     /locus_tag="ACFB64_000139"
     CDS             complement(18659..20572)
                     /gene="thrS"
                     /locus_tag="ACFB64_000139"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC1208599.1"
                     /translation="MLNITLPDCSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     EDMPEVEAMGIYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKDYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQTI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVRLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFVGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVIGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(20625..20701)
                     /locus_tag="ACFB64_000140"
     tRNA            complement(20625..20701)
                     /locus_tag="ACFB64_000140"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20665..20667),aa:Val,seq:gac)
     gene            20880..21995
                     /gene="tgt"
                     /locus_tag="ACFB64_000141"
     CDS             20880..21995
                     /gene="tgt"
                     /locus_tag="ACFB64_000141"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC1208600.1"
                     /translation="MLKFTLHKKDGLARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGETTREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEEFDFPGLAVGGLSV
                     GEPKPEMYRMLHAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQAQFHENRARGVD"
     gene            22005..23087
                     /gene="hemH"
                     /locus_tag="ACFB64_000142"
     CDS             22005..23087
                     /gene="hemH"
                     /locus_tag="ACFB64_000142"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC1208601.1"
                     /translation="MPSEAASDGILPPYPTCTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRIVELPKWLWYPILHGLVLTFRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSIADVLAELKSQGVGRLLA
                     IPLYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTISRFYDDAGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDEYTVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALTGREQFYEAGGKNYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            23278..23745
                     /locus_tag="ACFB64_000143"
     CDS             23278..23745
                     /locus_tag="ACFB64_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c2"
                     /protein_id="HFC1208602.1"
                     /translation="MNTTRLPTAFILCCLCAAASAADNSTMTKGQKVYESNCVACHGK
                     KGEGRGTAFPPLFRSDYIMNKPHVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKNQTDKMPSETGNPASDGIQIKPF"
     gene            complement(23742..25463)
                     /locus_tag="ACFB64_000144"
     CDS             complement(23742..25463)
                     /locus_tag="ACFB64_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFC1208603.1"
                     /translation="MNSLFLLGGALLFLSVVSTTLSARLGMPLLLVFLAVGMLAGEEG
                     VGGIAFNNVVMANFISQLALAVILLDGGLRTQLSSFRIALKPASVLASWGVFATVLPL
                     GLFATFYLGLDWKFGVLMAAIVGSTDAGAVFSLLRNSGVRLNERVQATLEIESGANDP
                     MAVFLVTALITMIMQPAESGAAAFVRMLALQIGFGLLTGWAGGKILAKLVRRLNLAEG
                     LYALMIVSGGLLVFAFTNTIGGSGFLAVYLAGIIVGNQRNRATEHVLRVMDGLAWLAQ
                     ATLFVMLGLLVSPAGVLDRAAEALAIAAFLMLVARPLAVFGGLWKFNYSLREKAYISW
                     LGLRGAVPISLAMMPLVMGVPNSHLLFDVAFAVVVLSLLIQGTTIPVMARLLKVAMPN
                     KPEPKDTHDIWLAEKEIVRMSAFKVVAESEAEGHHPDTVEPISDSFDARCFALIRNGS
                     RIEMQSDTVLQAEDLAWYILPDGKVDKMAKYFTETGIGVRENFDFFGEFVVSPAARSG
                     DLALAYGLKLEAGEEGLSLAELFDKRSDSQEPVEGDRIDIGGFMLTAKEVDGGGNIGS
                     MGLKVPR"
     gene            25667..26407
                     /locus_tag="ACFB64_000145"
                     /pseudo
     CDS             25667..26407
                     /locus_tag="ACFB64_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            26476..28014
                     /gene="lnt"
                     /locus_tag="ACFB64_000146"
     CDS             26476..28014
                     /gene="lnt"
                     /locus_tag="ACFB64_000146"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC1208604.1"
                     /translation="MFSKLDKYWQHPALYWPLLILFAAATPFAFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAASSAYLFGLTAYTAQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPQGIKTGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLAIDNTARSGKRLLPAILIAALLAAGYTAQQT
                     DFTRPDGSRSTVALLQGNIAQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPI
                     MRQNLPENILAQFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPLLTTPLYKMMDMPLSDFRKGGGKQSAMMMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANVSNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYIGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            28278..29141
                     /gene="rpoH"
                     /locus_tag="ACFB64_000147"
     CDS             28278..29141
                     /gene="rpoH"
                     /locus_tag="ACFB64_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC1208605.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNVLSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(29298..29684)
                     /locus_tag="ACFB64_000148"
     CDS             complement(29298..29684)
                     /locus_tag="ACFB64_000148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFC1208606.1"
                     /translation="MIFASRWLQVPIYAGLTVVRAICAYKFLKSLKHLVMNLDVSDEN
                     AIMLAVLNLIDVVMIANLLTMVQIGGYESFVSRLRIDDHPDRPEWLSHVNAPVLKVRL
                     SMSIIGIHPSICSKHLSIPPICRKSN"
     gene            29874..30758
                     /locus_tag="ACFB64_000149"
     CDS             29874..30758
                     /locus_tag="ACFB64_000149"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC1208607.1"
                     /translation="MSSGNIRIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLENGIREQISSHLARGKVECKIQIQDAENGNQSLELNRVLVGQLAEINKNLR
                     KHYDLAKLGVADILRFPGVLASQKENTEALAKTIVELADKALKDFTAARRREGKKLGE
                     HLLQRIKNMEEIIDALSEIFPALVEAHKEKIRTRLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(30849..31364)
                     /locus_tag="ACFB64_000150"
     CDS             complement(30849..31364)
                     /locus_tag="ACFB64_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC1208608.1"
                     /translation="MMILKTAPLKRRFAAILYEMLLVGAATCLAALLAGIAAIFLNPV
                     SIAVSALVTSILIMGAWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALVWLILPWGFALLNPDRQFLYDFLA
                     GTRLVDVKEQS"
     gene            complement(31440..31859)
                     /locus_tag="ACFB64_000151"
     CDS             complement(31440..31859)
                     /locus_tag="ACFB64_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208609.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(31980..32978)
                     /gene="holA"
                     /locus_tag="ACFB64_000152"
     CDS             complement(31980..32978)
                     /gene="holA"
                     /locus_tag="ACFB64_000152"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC1208610.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEAVDALRAAAKKQGY
                     LNREAYTADASFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKNGGEALQDFAARLP
                     EDTVTLVLLPKLEKTRLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHAVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLEEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLVPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKQLVVSLAE"
     gene            complement(32980..33459)
                     /gene="lptE"
                     /locus_tag="ACFB64_000153"
     CDS             complement(32980..33459)
                     /gene="lptE"
                     /locus_tag="ACFB64_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC1208611.1"
                     /translation="MNKIFLTAAALMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRILDYADNEILGKQEEEETLWAEMRQDVAEQIVRRLTFLKAE"
     gene            complement(33511..34290)
                     /gene="pgeF"
                     /locus_tag="ACFB64_000154"
     CDS             complement(33511..34290)
                     /gene="pgeF"
                     /locus_tag="ACFB64_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC1208612.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDDPETVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWSGLAGGVLQNTIAAMKVPPVEI
                     MAYLGPAISADAFEVGQDVFDAFCTSMPEAAAAFEPIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDIFFSYRRDGATGRMASLIWLDGNAV"
     gene            34443..34667
                     /locus_tag="ACFB64_000155"
     CDS             34443..34667
                     /locus_tag="ACFB64_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208613.1"
                     /translation="MMPLYTFSELYMLQQGTAHQAPHCVLPERGCPPIRFYRYKQTGF
                     NRKGMGIKSISDTSRAMPSENQSPLSDGIV"
     gene            34744..35691
                     /locus_tag="ACFB64_000156"
                     /pseudo
     CDS             34744..35691
                     /locus_tag="ACFB64_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(35844..36968)
                     /gene="rluD"
                     /locus_tag="ACFB64_000157"
     CDS             complement(35844..36968)
                     /gene="rluD"
                     /locus_tag="ACFB64_000157"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC1208614.1"
                     /translation="MQNTSFDNESDYSDDSDFASASGTENRIGLTVPPELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MDLDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAAVKFGGKPAVTHVKVLERYLAHSYIECSLGTGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            36967..37770
                     /locus_tag="ACFB64_000158"
     CDS             36967..37770
                     /locus_tag="ACFB64_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC1208615.1"
                     /translation="MKKILLTVSLGLALSACATQGTADKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAKKIIGSYQNTRYVEESL
                     AILELAYKKLDKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(37840..40059)
                     /locus_tag="ACFB64_000159"
     CDS             complement(37840..40059)
                     /locus_tag="ACFB64_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC1208616.1"
                     /translation="MFRRWFLPCWVVGVAVSFALSVVPHWPFWLAAFAVLAVLARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVTDMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGVGGV
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWRQADADGGLSDGIGLMRALSVGEQSALRP
                     GLWQAFRPLGLTHLVSISGLHVTMVAVLFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACAGRLYEGKRQTAVRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VSIPWFSWVLTPLALLGSVVPFAPLQQAGAFLAEYTLRFLVWLADVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVSYRPEAVPENEAAVTVWDAGQGLSVL
                     VRTANRHLLFDTGTVAAAQTGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLRPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRVYKFYWQKKPFE"
     gene            40091..40366
                     /locus_tag="ACFB64_000160"
     CDS             40091..40366
                     /locus_tag="ACFB64_000160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208617.1"
                     /translation="MIKQSHKTVITRFENDKNANEKPQYYPRQRQTFHRHSRKSGNPK
                     TKSRRNLSERTEPQKPASHLRLNNRTKKAEIKTSGFFFKLAIKHQNE"
     gene            40445..>43631
                     /gene="iga"
                     /locus_tag="ACFB64_000161"
     CDS             40445..>43631
                     /gene="iga"
                     /locus_tag="ACFB64_000161"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HFC1208618.1"
                     /translation="MKAKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKRGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYEPHKAWGASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLDTYKDKNRFSSFVRIGAGRQLVYEKGVYHQEGNEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNKQYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADKIKQHDNAGTVKG
                     NGEHHWKTTGTNSHIGSTAVRLANNEGDANNGQNVTFEDNGTLVLDQNINQGAGGLFF
                     KGDYTVKGANNDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQG
                     QLKVGDGTVILNQKADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRL
                     DANGNDLTFEHIRNVDEGARIVNHNTDHASTITLTGKSLITNPNSLSVHSIQNDYDED
                     DYSYYYRPRRPIPQGKDLYYKNYRYYALKSGGSVNAPMPENGVAENNDWVFMGYTQEE
                     AKKNAMNHKNNQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGANLNGKI
                     SVTQGNVLLSGRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEIAVNQ
                     SASFSSGRNVSDITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALN
                     SFDATRINGNVNLNQNAALVLGKAALWGKIQGQGNSRVSLNQHSKWHLTGDSQVHNLS
                     LADSHIHLNNASDAQSANKYHTIKINHLSGNGHFHYLTDLAKNLGDKVLVKESASGHY
                     QLHVQNKTGEPNQEGLDLFDASSVQDRSRLFVSLANHYVDLGALRYTIKTENGITRLY
                     NPYAGNRRPVKPAPSPAANTASQAQKATQTDGAQIAKPQDIVVAPPSPQANQAEEAKR
                     QQAKAEQVKRQQAEAGRKSAELSAKQRAGEEE"
BASE COUNT        10943 a        12186 c        10274 g        10228 t
ORIGIN      
        1 cacaatcact tgttcaagtg attaagaaag cgtgataata cggatttatc tgagtcctgt
       61 ctattatttt agacttctat tttcagttat ttttgtaact gattgatttt aaaataaact
      121 atttttcgac gcaagttgca gcaattctgc cgcatgttgc cgcgtcgtat cggtaataac
      181 ttctccgccc aacatacggg caacctcttc gatccgttgt atttcatcca atatactgat
      241 ttcgctgacg gtttgctctc cctcgctgtg cttgcgcacc cgccagtggt tttctccgca
      301 ggatgcgact tgcggaagat gggtaacggc aagcacctga tgttttctgc ccaacgcacg
      361 taatgccttg ccgaccattt cagccactcc ccctccaata ccggtatcga cctcatcaaa
      421 aatcagggtg ggaacttggg tatattggct ggcaacaacc tgtaaggcaa ggctgatacg
      481 cgccaattcg ccgccggagg cgactttgtt cagcgggcgg ggcggattgc ctttgttagc
      541 ggcaacttga aattgaacct gctccaaacc gtgtgccgtc ggcgacgaag gcaacaggac
      601 gatgtcgaaa cgcgcgcctt tcatggcaag gtgttgcata tgctcggtcg tttcgctact
      661 caaacgccct gccgcctgat ggcgcatggc agaaaggatg tgggcagctt cctgatattc
      721 ggcaaaattg tgggcaacat tatgctcgag cgcgtccaaa tcagcggcag cttgaagact
      781 ttgcaggcgt tcttcgattt ctgccaactt ggcaggcaac tcttcgggtt caatccggta
      841 tttccgcgcc atccccatca gttcgcccat acgctgttcc tgtgccgcca attcattggg
      901 gttgatgtca ctgtgtcctg ccacatcgcg catattggca ctgatttcgc ccaattcggc
      961 ctcgatgctt gccaacatat tcaggctctc ggcaaagcgc ggctcgatgt tttgcagatt
     1021 ggccaatagt ttttgacatt gatagatatg gcgttggatg ccgttgtcgc cgtcaatctt
     1081 gcttccgact tcttcggcgg cctgcaacaa ttcggcagaa tgggcaaggc tgtcgtggct
     1141 ttggctgaga gcttcccatt cgccttgttt aatgtccaac tgattcagtt cgttaaactg
     1201 ccattccaga cgctcccgct cgataatgac ggcatcggcg tgttcctgcg cctcttggag
     1261 ggcttttttc gcattggccc agttttgata aagctgcctg acggtttccg cctgcaccct
     1321 gccgcccaca aatgcgtcca acaattcgcg ctgggcagct tcctgattaa gcgaatgatg
     1381 gtcgttttgc ccgtggatgt cgataagctg cccgccgacg gctttgagtt gcgccaaggt
     1441 agccgcctga ttgttgataa aactgcggct tttgcctttg gcatcgataa tgcggcggat
     1501 actgagttct tccccgccct catccaacag cccttgttca cgcaattctg cttttaaagc
     1561 aggcaaatgg gaaatatcga acaatgccga caactgcgct tcttttgcgc cgctgcggac
     1621 ttggctgtaa tcggccttat cgcccaacag cagaccaatc gcatccaaag taatggactt
     1681 gcccgcgccg gtctcgccgg tcaaaacggt aaaaccgctt tgaaaatcca gattcagatt
     1741 ttcgacaatg acaaaatcac gcaaagaaag tgttagaagc attgtgagga ttccttagca
     1801 attctaccga caaaagcaat atcaacttta gatatacgaa ttatatctaa gattttttaa
     1861 attaaaaata agtttttcct taattttgct tagtgtttgt ttttatctgc ttatttgttg
     1921 caatgccgtc tgaagcaatg tgcgtttcag acggcatttg gaatttcagt tgggcagggt
     1981 atcggacggt acggtttccg gctcttcttc atccgtaggc ggcatttcta tcaaatcggg
     2041 caaaaccagt tcgcccagtt cggtcagcgg cggcagttct tccaaaccgt ccaagcgcaa
     2101 atcgctgaga aaagttgccg ttgttgccca caatgcgggt tttcccaatg tgtcccgatg
     2161 tccgatgact tcaatccacc cccgatcctg caaggtctgc atcacgttct gcgacaccgc
     2221 cacgccgcgt ataccttcga tgtcgccgcg cgttacgggc tgctggtagg cgataatcgc
     2281 cagtgtttcc atcacggcgc gggagtagcg cggcgcacgc tgttcttgca ggctgcccag
     2341 ccgctcgaat gccgtctgaa caatctgaaa acgccagccc tcctgcgtat gcaccagttg
     2401 caacgcccta tcctgccaac gcgttttcaa ctgcgccaac acatcaatca gtttgtcttg
     2461 cgacaacggc ggcacacaca gttcgcgcat agatttttcg gtcagcggtt cggtttgggt
     2521 cagcagtgcg gcttcaatca gcgcgtcggg agaaattttg tcggtcatac gggtatccgt
     2581 gctgaaacgg catgggtttg gatatgccgt ctgaaatcgg ttggagtaga gagaagctgc
     2641 ctgaaaaata tttttcagac ggcattcttt atgcttccga aggcttctgc cctttgcgct
     2701 ctgcttttct ggcttcgcga atggctttga gttcggcttc tttctgacgt gcttttttaa
     2761 gccaagtttc ccattggttc ggtgtttcaa gggtgattct gccgattttg ccttcgcgga
     2821 agtccgtgag gatgttttcg gcggcttttt ggtagttgac ccgtccgccg ctgaggactg
     2881 cgccgcgttt tttggctatc cattcgagcc acgagttgtc gtcccagtgg ctgctggggt
     2941 ctttgtcggc ttggtagcgt tcttgcagca tagggaggta gtggcggcgg aggtagtcta
     3001 aaagttcgag ggcgacttct tcttcgtcca atgcgttgcg tccgactgcg ccgccggcgg
     3061 cgaggttgta gccgctttct tcgacgatga ttttcggcca cagcattccc ggggtgtcgt
     3121 acagccagaa gtcgtcggca aggaaaaggc gttgttcggc tttggtaatg ccgggttcgt
     3181 tgccggtttt ggcggatttt ttgcctatca ttccattgat gagggtggat ttgccgacgt
     3241 tgggaatgcc gcaaatcaaa acgcgcaggg gtttgtctat gccttgccgg tggggaatca
     3301 tcgcgcggca ggcttgggtg attttgccgt gtgcgccggt ttcggaggaa tcgagggcga
     3361 tggcccgggt gtcggggcgg ctgttgtagt ggtcaagcca gacctttgtg cgttcagggt
     3421 cggcaaggtc ttgcttgttt aaaattttga gtttgggctt acctttggaa agctgcgcca
     3481 acagtggatt ttcgctggag gcgggcatac gcgcgtccag catttcaatc accatatcaa
     3541 cgcttttggc acgctcggcg atagcttttc ttgccttgtt catatggccg ggaaaccatt
     3601 ggattgccat gtctgttctt tcttctcaat atttgaaatg ccgtctgaaa cggcggacgg
     3661 ggtttcagac ggcataatgg tttgacggga ttagcggccc gacaggtttt gcgcctgcct
     3721 gtgccgtatc agcaattcat aacgcccttt caaccttcgg gcgagttttt cgacaatata
     3781 gaccgaacga tgctgcccgc cggtacagcc gatggcgacg gtaacgtagc tcctgctttc
     3841 atcctccaaa cgcggcaacc aacgcgtaac aaacctttcg atgccgtcaa ccatttcctg
     3901 cgccaacggc tgtccgtcca aataatccca aacgggcttg tccataccgg tgtaaggcct
     3961 caactcggga tcgtaatacg ggttgggcag actgcgcata tcgaacataa aatcggcgtt
     4021 gttcggcaca ccgtatttga acccgaagga ctccaaaacc accagcagtc cggtacgctc
     4081 aaccttcagc cactgccgga ctgcatggcg gagctgttgg gcattcatct tggaagtgtc
     4141 gatacagtag gcgatttcct taagcgggaa cagccattcg cgctcttttt tcaggctttc
     4201 caacagcgtc atatcctgat tgctcaaagg atgtccgcgc cgggtttcgg aaaagcggcg
     4261 gaccagcacg gcctcttccg cctcgacaaa caaaacttcc accctgtgtc cgagtccgcg
     4321 cagataggca atctgctcgc gcgcttgggc aatgtctatg ccggaacgca catcgacgct
     4381 gaccgccaat tcggtttcgt ccgcacgttc gatatggtac gacaccagcg acggcagcat
     4441 ttccaagggc aggttgtcca cgcaaaaata gcccaaatct tccatttggc gcagtgcaac
     4501 ggatttgccc gagccggaaa gcccgctaat caggacgatt ttcatgattc tgttcgtttt
     4561 ctttaagttg cgtctgatgg cgttccaaaa attcgcgcgt actgtctttg ccgcgcaact
     4621 gcaaaatgta attgcgtacc gccgcctcaa ccaaaacggc aaggttgcgt ccgacggcga
     4681 cgggcagcgt aaccgaacgg acgttgacgt tgaggatgga ttcggtttcg gtgcggatgc
     4741 tcaaccggtc gagctgcttc atatactcgt cgtccgcctc gaccaaattg ataatgagtt
     4801 gcaggatttt tttggggcgg atggaagttt cgccgaaaat atggcggata ttgagtatcc
     4861 ccaagccgcg cacttccaaa aaatcccgca gcataggcga acaacgccct tccaatgttt
     4921 ccggaccgat gcggaacagc tcgaccgcat cgtcggcaat caggctatgg ccgcgcgaaa
     4981 tcagttccaa tgccaattcg ctcttaccca aaccagaatg tccggtaatc agcacgccga
     5041 tttcaaacac atcgagaaat acgccgtgtt tgacggacga tgctgccaag gtgcgttgca
     5101 ggtaaatccg caacacatcc atcagataag ggctttcgag tttggaagtc aacaggggga
     5161 tgtcgttttt gtggcagtag tcgcgcagcc ccggggaaac cggcaaatcg tttgccacaa
     5221 tgaccaaaga catggaaata tcgaacaggt ctccgaactg ataacccgtc tcccccgatt
     5281 cgaggcggtt cagatattcc gactccgcca aaccgaccac ttggatttgg ttgggatgga
     5341 tgaaattcag gtgtccgacc agggcgagga cgggcttgtc cgcctctacg ccgatacggt
     5401 tgtccgcacc cgaattgccg gcggcccagg caagttgcaa cttgtattgg ttgtcgtcaa
     5461 acaggcggcg gacggagata ctgggcatat tttattcttc ggtcaggatg gcgcgggctt
     5521 cttccgcaga agcaaccgtc atcagcgatt ctctgatgct tttttgggaa aacttgccgg
     5581 ccagtttgga taagacttcc aaatgctcgc ccgttgcgtt ttccggcacc agcaagataa
     5641 aaaccagtga aaccggtttg ccgtcaggcg cgtcgaatcc gacgggttcg cgcgtacgga
     5701 tgaacgcgcc cgtcgcctgc ttcacgctcg cgtggcggcc gtgggggatg gcgacaccct
     5761 gccccaaacc ggtcgaaccg agtttttcac gggcaaaaag acattcgaaa acatcagcat
     5821 gggacaatga ggattcgcgt tccaaaagca ggcccgcttc ctcaaacagc ctttttttac
     5881 tgcccacctc catatccaaa acaatatggg acaaaggcaa aatttcgccg ataaggctca
     5941 taagcttctc ttttcagaca tcgcaaaaca gaaggattgt accgactgcc ggggcaaacc
     6001 tcaatcccgc atacggtacg ggctgacata acacagcgtt tcaaaaacat attttaacgc
     6061 ttttcggcac agatagaaat gccgtccaaa gcagtttacg gttcttcaga cggcattgcc
     6121 ctgccttatt tcgcgaaaaa cgtatgggca aaaatagcgg caaaccacac ccaaccgatt
     6181 ctgttgtttg ccaaaaacgt ttcaaaacag atttgccgga cgcggctttt gatggcggca
     6241 tattggcggt attgcagcag caggacgatg gggattgccg tccaatatgc ccatgccgca
     6301 ccgataaccg cacccaatac tgccatcagc agggtaaagc cgccatgaca cagcataacg
     6361 gcggcgatgt cgtaacgccc gaacgtgacg gcggaggttt tgatgccgat tttcaaatcg
     6421 tcctctttgt ccgccattgc atagacggta tcgtaagcca gtgtccataa aacattggcg
     6481 gcaaagagta tccacgcttc aacgggaacg ctgttgccca ctgcggcaaa cgccatcggg
     6541 atgccgaagg aaaaggcaag tcccagataa aattggggaa tcggaaaaaa gcgtttggta
     6601 aacgggtaag tcagcgcaag gaacagcgcg ggcaggctca tcagccatgt cagatgattc
     6661 agcggaatca ggcacaatgc ggcaagcaga cacaaaaatg ccgtcagcag cagggcttct
     6721 tttttcttga ccctgccctg tgcgaacgga cggtttttgg tgcgctcgac agcaccgtca
     6781 aaatcgcggt cggcaaagtc gttgatgacg cagccggcac tgcgcattaa aaacgtgccg
     6841 attgtaaacg ccgccaatac cgccaaatcg ggaatgccgt ctgaagccag ccacaatgcc
     6901 cagtaggtcg gccacagtaa aagcagcgtc ccaatgggct tgtccgccct catcagtctg
     6961 atataaacgt ccaaacgatc ggacagtcgt aaaaataaag gggatttaag attcataacc
     7021 ctctttcata taagtcttac acccttggaa aactaatcca taaacttcta gttccaataa
     7081 ccaatttgaa ttattgtcgt aaaatcttat tatttttaga ccatcttctt ggttctttat
     7141 ttctacttgg catatatttc tattgttatg cgaccaattt tgacatttca ttcctttaat
     7201 aaagctattt cttaattcaa tttcaactgt atcataatct tttagccaca gcccccattt
     7261 ttcgatttct acaaaaggct ttgtacttgt tcggatagtt aaaatacaat tagacaagtc
     7321 agttgaaaaa gagactagtg aaatatccat tatgccacta ggatatattt gttttagaaa
     7381 acgattccta cttattattt ctattatatt catatttctt actaaaaatt atagtgtccg
     7441 tgagtaccat cgacatgtcc tgtatctaaa ttggagtctc ttatattaaa atgtggttta
     7501 gatgcaggtt gccgcacagc ttgaaaaaac ggtattttat ccgataaagc gtttcagttc
     7561 gggcagaaaa tactcggtca gcagcatttc ctcgccttga tgggaaaacc gagaacgccg
     7621 cgcggcaaag taccgtccgc atccttcact ggaaacggca aactcaaacg ccgaacgcgc
     7681 cccttccaaa tcggcttgaa acagacgctc gcccaaaggg cgtgtgccgc agtccaaaat
     7741 gttttgccaa aacgccgaac cgacgctgca cgcgcttcgt gcctgcacaa cagcagtgcc
     7801 gtccagtttc agaagcacat cgcgcaccct ccgccgtccg tattccgttt ccgcttcgcc
     7861 cagtttcagc agttccaccg aaaatgtatg cggcaaggcg cacaatgcgg cggtcagcga
     7921 ccgggctttc aataaccggc tcatcggcag gtcgatgccg tctgaaacgg gggcgggcaa
     7981 gtcgggcagc catttcccaa ataggtgttc catatctttc ccaatcttta taccgcgcct
     8041 gtttttgcca actccatcca ttccgcttcg ggaaaatcgg ctttcagacg gcattgcaga
     8101 tagttcaggc tgtcttgggc gacgtgtgcg tcgcggctgt cgtggatgtg gttaaacacg
     8161 aggctgtgca ggcgaatgcc gtattgtttg agcacgacga aactgagcag ggtgtgatta
     8221 atgcttccca gccgtccgct ggtaacaagg atgacgggat aagcctgttg ctgaatatga
     8281 tcaatggtta acagcttttc cgtcagtggt accatcaacc cgcccgcgcc ttcgaccaaa
     8341 acgacttcgt actgcgccgc caattcttgt gtggcggtgc ggattttgtc caagtccaaa
     8401 cccctgccgt ccagtcgggc ggcaaggtgg ggcgaggcgg gatggctgaa gatttcaggc
     8461 atagtcagac gctgttcatc agcttcctgc atgggtatgc ccatgatttt acggtggacg
     8521 gcgatgtctt cggcgatgtc ttggcaaccg gtttgcacgg gcttttgcgt aatcacgctt
     8581 ttgccctgct gcaacaattg ttttgccaac ataccggtgg cgacggtttt gccgatgtcc
     8641 gtgtctatac cgctgacgaa gtaaacgcct ttcatttgct gtgttccttc aagatttgca
     8701 cggttttatc ggcaagtttg gtcaaaatgc cgtctgaaat gatatagggc ggcatcagat
     8761 acaccagcct gccgaacgcg cgtacccaaa tgccctgcgc cacacaatcc gcttgaaaac
     8821 gcgccatatc cacgcctttt tccaactcta tcacgccgat agcacccaaa acgcgcacgt
     8881 ctttcacgcc gcgaatgtcc cacgcggctt tcagacggcc ttttaagatg ctttcgatac
     8941 ggcggatatt tgcctgccag tcttgggata aaagcagctt gaccgaagca caggcaacgg
     9001 cacacgccag cgggttcgcc ataaatgtcg ggccgtgcat aaacacgccc gcttcgccgc
     9061 gtgaaatcgt ttcggtaact ttttgcgaag tgattgccgc cgccagcgtc atatagccgc
     9121 cgctcaaacc cttgccgata cacataatat ccggcacgac ctccgcgtgt tcgcaggcaa
     9181 acatcttgcc cgtgcgcccg aatccggtgg cgatttcgtc aaaaatcaac acgatatcaa
     9241 attcgtcgca caaatcgcgc aagccgcgaa gatactgcgg atgataaaaa tacatgccgc
     9301 ccgcgccttg cacgaccggt tctaaaataa aggcggcaat atccacatga tgcgcttcaa
     9361 acaaggcgcg gacaggctgc aaatccgccc cgtcccattc atcgtcgaaa cggtttttcg
     9421 gattatcgac aaaataacgc tgcggcaacg cgctgccgaa aatatggtgc atccccgttt
     9481 ccggatcaca aacggacatc gcgttccaag tatcgccgtg atacccgcgc cgtaccgtcg
     9541 caatattctg cttcgccgtc agaccccgcg cctgctggta ttgcaccgcc attttcaacg
     9601 cgacttctac cgaaaccgaa cccgaatccg cataaaaaat acggtccagc ccctgcggca
     9661 aaatcccgac caacaacttg cccagctcca ccgccggctc gtgcgtcagg ccgccgaaca
     9721 tcacgtgcgc catttgtttc atctgattct caaccgcctg attcaaaacg ggatgattgt
     9781 aaccgtgtat cgcacaccac caagacgaca tcccgtcaat cagccgcgtg ccgtccgcca
     9841 attcgataaa caccccttct gcacgtttga caggataaac gggaagcgga tcggtcatgg
     9901 aagtataggg atggagcaga tgggtacggt cgaaattaag taatgatgat gtgtgttgat
     9961 gttcagacgg cataagtttc tctcttttct tattactgca ttcaaacgca aaaagcgtat
    10021 tctactccga cagaccgctt cccacacctt tccatccgtt tcgggcgaaa aaccgcgaaa
    10081 caaatcgtcc gcaacataag cgcacaccgt ttcgcattcc tcaagcctga ttggaatcag
    10141 acgacccaac gcccaatacc gtttcacaca gcctccgccc aacgcttcaa tcaatcatag
    10201 ataaatacga tacagatcat agcaacagcc atcgcaacag cgttggcaaa atcaggggac
    10261 tccggcatag gcgcatagca tctgccgatg cacggctcct cattcggctc tatgaatacc
    10321 atacccatca caaaatccac tgctaaaacc aggcacggct tcttatactt atgatagatt
    10381 tccaccatcc tgtcccatat atcccaaaca ctcataccgt atatcccgca ggcaacaaat
    10441 tccgattgaa ggttacagcc ctattttata gtggattaaa tttatagtgg attaacaaaa
    10501 accagtacgg cgttgcctcg ccttgccgta ctggtttttg ttaatccact atattttcaa
    10561 accggaaaaa tgccgtctga accttcagac ggcattccat tcatatttat tcgtcttttt
    10621 tgtgttcgat ggcgtttcgg gaacgattgc cgccgtaagg ctcttcaacc gtatattctc
    10681 cctcgataat atcgtcatcg tggaaaaatc cctcttttct gcccgattgg ttcatgttga
    10741 aaaaattttc cgcacctcct gcctgcaaca ctgcccctcc cttaaacggc agcagcagca
    10801 ataccgccaa caccgaggat acgaatcccg gactcatcag acacaccgcc gccaccgtat
    10861 aacggatagg ccacaacatc tgataaacag ataccttccc actacttttt accgccgcgc
    10921 cagccaataa aagaccggac agccccgtat gcctgagcat cagcacaccg gcggcaaagg
    10981 ttgccgccat tagaaacagc gtccaaccgc cgcccagcca atcggcaacc cacacaatcg
    11041 acataatttc caaaaacagc agcaccaaaa aaccgatacc gaaaaatctc attgaccgtc
    11101 atccttatat ttaagtaaac agcaaaccgc ccgaacagga ttccaagcga gctgcctgta
    11161 aatgattaca aaaccatgtg cttcaagccg aaacaatgtg aaatcacgca ataattattt
    11221 ttagcgtgta aaacaaaacc ggctgcatac ctgcaaccgg tctcaaatca gcacaactcc
    11281 ttatccaaat cctccaacag gtctttcaga ccgtctccga tttcctgaag cgtaaccgga
    11341 cggttgccgt ccgaacccga ttccgcacct tctgcatacg gtgcaaacgt gctcttcagt
    11401 ttcaacagtt tcaccacatc caaacgggtg tagcgttctc cgccgtaacc gacaaccaca
    11461 ccgtgatcat gttgccattg cgcaaaacca taggggctga tttgcaacag tccgcacaac
    11521 tcgtccagcg tgaaatagcg ttttgcagga atcgtcaaat caacgttgtt tgtcatagta
    11581 gtgttccacc atgcctttga gtttctggct ggcatggaaa gttaccacgc ggcgggcggt
    11641 aatcggcact tcctcgccgg ttttcggatt acggcccggg cgttgcggct tgtcgcgcaa
    11701 ctggaaattt ccgaaaccgg aaattttgat ttcttcgccg cttgccaaag tgctgcggat
    11761 ttcttcaaaa aagagttcga caatttcttt ggcatcgttt ttggtgacgt tgctgacttt
    11821 atccaccaaa atatcggcca gttctgcttt agttagtgtc atatgattac cttctgttgt
    11881 aacagttgtg gattattacc aatttttatt taaaattcaa gcgaatattt attttttaac
    11941 tgcgaagccg cgcccctgcc tccgttgcca cgccaatcag ttttccaata agcggctcga
    12001 ccgcctcatc cgtcagcgtg ttttccatat cctgcaaaat cactttaacc gccacgctct
    12061 tcatcccttc gggcagaccc gtgccgcgat acacgtcaaa cacgctgatt tcctgtacca
    12121 acttgtttgc tgcgcctttc aagacaagca gtaaatcatc atgattcata acttccggca
    12181 tcacaaacgc caaatcgcgg cgcaccggct ggaatttcga tacggcccga tagcgcgttt
    12241 tcccgcattc caacacagcc gccatatcga tttcaaatac cagcggcgct tgcggcaggt
    12301 cgtatttttg cagccatttc ggatgcagtt cgccgacaaa gccgatgact ttgccgtctg
    12361 aaacgatatt ggcggcacgt ccgggatgca gggcgggatg tccggtttta acgaactcga
    12421 ccgctttgtt tttcaacaga ttttccacgt ccgccttgat gtcgtaaaaa tccgcattgc
    12481 gcgttttctc accccattgt tcgggcatcg ccgcgccgta ccacagaccg ccgatgcgtt
    12541 cgttttggac aaaccggccg tctgaacctt tgccgaacac gcaggcgatt tcaaacacgc
    12601 gcacgcggtt ttgcttgcgg ttcaggttgt tttgcaaaat ttccactaag ccgccaatca
    12661 acgtagaacg catcacggca tattgcgccg ccagcgggtt ttgcaggcgg atgggggcgg
    12721 cgttggcggc aaaatcgagt tcccactgct cgtcaacgaa ggcatagctg atcacttcgc
    12781 ggtagccgcg cgccgccatt tcgttgtaaa cggcaaaacg cggacggcgt gtttcgggca
    12841 gttccgacat tttcagacgg cctgacgtgt aatcgtcggg gatgttttca tagccgtaaa
    12901 cgcgtccgat ttcttcaatc aaatcagcct caatttcgat gtcgaaacgg aagctcggcg
    12961 cggtaatgcg gaagccttcc gccgttttct caggctgcag acccaagtgt tgcaaaatgg
    13021 tttccacctg ttcggcagga atgtccacgc ccaacacggt tttcagacga cccaaacgca
    13081 atccgacctg cttggcttcg ggcaatttac cttgtgcttc caccatctcg cctgccgcac
    13141 cgccgcaaat ctgcaaaacc aattcggtgg cgcgttcaat ggcatccgcc tgcaaacggt
    13201 aatccacgcc gcgctcgaag cggaacgacg aatccgaacc gaaaccgtat tggcgcgatt
    13261 tgccgacgat gatttcgggc tcaaaccaag ccgcttccag cacgatattt tgcgtgccgt
    13321 ctgaaaccgc gctcgccgcg ccacccatca agccggccaa gctcaacgca cctttttcat
    13381 cagcgaccac cagtgtattg tcagccaaag taaccgtctt ctcattcaga cacgccaaag
    13441 tctcgccatt ttgagcacgg cggacaatca aactgcccga cagcttatca gcatcgaaaa
    13501 catgcatagg ctgaccgatt tccagcatca catagttgcc aatgtccacc aatgcggaaa
    13561 tgctgcggat accgctgcgt tccaaacgtt gtttcatcca atcaggagta gccgctttcg
    13621 cgttgacatt ttcaataacg cggctgataa aacggccgca gtcagccggt gcatcaatac
    13681 ggacagcctg ttttttctca ctgccgatag gggccgtctg aatttcaaca ggcgtaaacg
    13741 cacattgagt caacgcagaa acctcacgcg caatgccctt aacactcaag caatcagcgc
    13801 ggttaggcgt aattttcaac gtaaacagca tatcgtccaa atccaagtat tcgcggatgt
    13861 tggtaccgac aggcgcatct tcaggcagaa tgtgcaggcc gtctacacca tcatcaggca
    13921 aacccagttc attggtcgaa cacagcatac cgtttgatgg tacgccgcgc attttagtcg
    13981 gcttaatttt aaaattgccc ggcaaaacgg caaccggcaa cgaacacggc actttaatgc
    14041 ccggcttgac attcggcgca ccacaaacaa tctgaaccaa ctcgcccgta ccggcatcaa
    14101 cttgagtaac gttcaaacgg tctgcatcag gatgtttttc aacagatttt acttcggcaa
    14161 caaccacgcc actgaaagca ggggcggcag tatcgatttc ttccacttcc aaaccggcca
    14221 tggtcaaaag atgttccagt ttatcggcag aaagatcagg attggcttgg gttttcagcc
    14281 atgagtagga gaattgcatg ttatgttctc aatcaattaa ttaacttttg ttttatcaat
    14341 tttcgttcag acagtctgtt ttagaattct ggttaatgtt gatatttact caactctgaa
    14401 aaatattttc tctgtgaaat tttaaaaatt ccagacttgg gtttagtctt tcaggcagtc
    14461 ttatcatccg attttcatat ggtttaaata aatcatcaaa tccttctgtt tttgccagta
    14521 gtggagaaaa tataattttg aaattttcat ctattcctaa caacccccta tcaaaagctt
    14581 tgtcatgtaa cgcattcaaa catagcccat tccttgggtt taaacggtta tccttatctt
    14641 ccccccaggg tttgatatga ctagcaacta acaattcagg ttgttttaat ccagtaatac
    14701 aacattgatt attgtatgct gccaaaacac tggaacggaa aaagccctga ttgacgcgta
    14761 ctgatattct tgcaaacctt tcccttccct ttggaatctc aggtgattct atttcaatat
    14821 tactattctc atcatttgat tggtattggg aaatgactcc ctcaaattct ttattaagtt
    14881 gctcccaatc tcctgaaaac tcattccata cgacaacatc cattttgctg ccattactta
    14941 atcccgaaat atttttgact ttcagcgtag gatctaaacg acccaaattt cctatcttca
    15001 taccgagtgc ggacggtgtc ctgcccagta tttcagcata ttcttgaata acaggattgt
    15061 ccttactaac cttttgaaaa ggaatgatgt aataaagata aagggcaacc agggtttcat
    15121 ctctagtcca ttttttcatt tttatcagct atctttctga ttttccataa ggaaatcgta
    15181 caatacagat tttcttattc cctgaacata ttgctgtcca tctagtacta atttgtatag
    15241 gtatacatca taatctttta actccttctc gcttgccaat aaccagtcgt ctatttctga
    15301 agaaaaatag tcaataattt cttgtacctg tttatcagat aaattattta tatttatttt
    15361 attattcaaa atataatcag aaagcctcat atttctaaaa tctttatttt gataaggtaa
    15421 acccacacag cacatcaaat tgcttcggaa tgtattgttt gatttcttcc aaagtaatat
    15481 cgccaaacgg cacttctccg aaattggctt cgtacgtcag tttggctttt tcgtcccact
    15541 cgctcgaaaa cacgtattcc ccgcccaagt tctgcatcgc gatgcggaag cccccgatac
    15601 ctgcgaacaa gtcgataaag gtaaatttgg gtttcaaggc cgtctgaaaa gtatcgttgt
    15661 tttcaaaaag gcggtattgt gctgcttttt cttttaaccg ttcatcctgc ccatcttcct
    15721 taatcttgtt tttgtaaatc aattcatctt gtttggatga tttctttgac tttttcggca
    15781 cagcaggttg tatttttgac gacatcgtta ctccaaaaac gtaaaaccgt ccagccctcg
    15841 gctttcagac ggcctgttac ttctatatcc ctttggatat tgcgctcaat tttggcaatc
    15901 caaaactcac gatttccctt tattaccgct tttttctgtt cccaatcctt accgtgccag
    15961 aattccccat cgacaaatac ggcaacttta tattttttaa atgaaaaatc aggttttcca
    16021 aaaatgcttc cgctattttt ccgatacctg agccccaaag cccacattgc cttcgccaac
    16081 acaagctcgg gtttcgtccc tgtacttttg ttggactgca tacatttttt gcgctgctcc
    16141 ggggttaatt tatccattgg gtttatttga gatttattca aactgcttca agaagttcaa
    16201 atcgttatcg aagaacaagc gcaggtcatt gacgttgtag cgcaacatgg caaagcggtc
    16261 gagaccaata ccgaaagcga aaccggtata tttttcaggg tcgatattga catttttcaa
    16321 cacattagga tgtaccatac cgcaaccgcc tacttccaac catttgccgt tttctcccat
    16381 gatgtcgatt tcggcagaag gctcggtaaa cgggaagaaa gacggacgga aacgcacttg
    16441 taggtcatcg cgttcaaaga agcgacggat aaagtcagtg aacactgctt ttaagtcggc
    16501 aaaagttacg ccctcttcta cccacaaacc ctcggcctga tggaacatag gcgagtgcgt
    16561 ggcatcgctg tccacacggt aaacgcggcc gggggcgata atgcggatgg gcggctcttt
    16621 tttatcgagc atatagcgga tttgaatcgg ggaagtgtgc gtacgcaaaa catcgccgtt
    16681 ttcaacgtaa aacgtatcct gcatcgcacg ggcaggatgg tttgcaggga tgttcagggc
    16741 ttggaaattg tgaaaatcgt cttcgatttc aggcccgtcc gccacttcga aacccattcc
    16801 gtgaaagagt tcgaccacac gttgcaaggt cagggttacg ggatgcaggc tgccgccttc
    16861 ctgagcgcgt ccgggcaggg taatgtcgag ggcttcggcg gcaagtcggg cttgcagctt
    16921 ggcttcgttg agggcatcgc gtttggcatt aaaagccgtc tgaaaccggt ttttgcattc
    16981 attgatatgc gcacctatgg ttttgcgctc ttcaggcgac atttgcccca aagttttcag
    17041 aagtccggtc aactcgccgg ttttgccaag ataacgggct ttgatttgtt ctagagcgtt
    17101 gaagtcttgc gcagcttcta ctgcggcaat gccttctgca acgatacggt ttacattttc
    17161 cataatatca agcctgtcaa ttaatgatat gccacctgaa acaacgaggc cgcctgtata
    17221 gcctgacata tcagactctt cagacgacct tattttcttc acggccaatc cattggataa
    17281 attttctgta ttttaacgca aatttattta aaaaaataga ctttaaagaa agaaaatcaa
    17341 tcacatagaa tttccgcaaa caaagaaaaa aggaagccgc agcttccttt tcaatttttt
    17401 ggattaagca gccaaagcag ctttggcttt ttcaaccaat tgtgcaaaag cggctttatc
    17461 gaacacggcc aaatcagcca atactttgcg gtcgatttcg atagaggcgc gtttcagacc
    17521 gttcataaat ttgctgtaag acaacccgtt ttcacgcgca cctgcattga tacgaacaat
    17581 ccacaattga cggaattggc gtttgcgttg gcggcggtca cggtatgcgt attgaccagc
    17641 tttcattacc gcctgcttgg caacgcggta aacgttttta cgacgaccac gatagccttt
    17701 ggctaacgcg aagatttttt ggtgacgggc acgggcggta acaccgcgtt ttacgcgtgg
    17761 catattctaa actccttaag cgtagggtaa cattttagca acagaagcca aatcgcgatc
    17821 atttaccata gaggtaccgc gcagttggcg tttgtttttg gtggtctttt tagtcaagat
    17881 gtggcgtttg aacgcatgag cgcgtttcac accgccgtta cccagtactt taaagcgttt
    17941 tttcgcgcta gacttggttt tcattttagg catgggaaaa ctccattcgt tatcggataa
    18001 ggcattaggg ggttttaaaa catcggtttc aaacacttga acccacaatg ccacggtttt
    18061 ttgccgcaat tgaaaactta ctccgaagcg gcaatcggag taagcggaaa attatagctt
    18121 tattttttct tcggcgcaat catcatcacc atttgacggc cttccatttt gggaaaggac
    18181 tcgatttgcg ccacttcagc caaatcttct tttacacgtt ccaaaagttg cgcgccgagt
    18241 tgctggtgag ccatttcacg gccgcggaaa cgcaatgtca ctttgacttt atcgccgtcg
    18301 gcaaggaagc ggttgatgtt gcgcatcttg atttgataat cgccctcatc tgtacccgga
    18361 cggaatttga tttccttgat ttgcacctgc ttttggtttt tcttggcttc gtcgcgcttc
    18421 ttggcttgtt ggtatttgta tttaccgtaa tccatcagtt tgcacacagg aggtttagca
    18481 gttggggaaa tctctaccaa atcgacatcc tgcccttcgg ccatagccaa agcttcacga
    18541 actgaaacga caccaagctg ttcgcctgac tcactgatta aacgcacttc tttggcggta
    18601 atttcaccgt tgattcgtgc ttcgcgttct tgagcgatga tgaatactcc tataaaaatt
    18661 aatgattgac gagggcatca gtgatttctt gctgcaattg cgcaatgaaa tcatccaaat
    18721 ccaaagaacc caagtcttcc gctttgcggc gtaccgcaac tttgttttct tgtttctctt
    18781 tatcgccgat aacgatttga taagggaaac ggtattggct gttgtcgcgg attttgtaac
    18841 cgattttttc attacgcaaa tccaactcgg cgcggaagcc tgccgcctgc aatttggcag
    18901 caacttcccg acaataatct gcctgatttt cggtgatgtt cataatcacc atttgaaccg
    18961 gtgcaagcca taacgggaaa gagcctgcat ggttctcaat cagaatgccg acaaaccgct
    19021 ccaaagaacc taaaatggcg cgatgcaaca taacaggccg cgctcggtcg ttgttttctg
    19081 ttacatattc tgcatccaag cgttccggca agacgaaatc cagttgtaat gtaccgcatt
    19141 gccaagaacg acccaaggca tctttgacgt gatattcgat tttaggcccg taaaacgcac
    19201 cctcgcccgg caattcgccc cattccacgc cgcaggcagt caatgcctcg cgcaaaccct
    19261 gctctgcctt atcccacacg tcgtctgaac ctgcgcgttt ttcagggcga agggaaagcc
    19321 tgacggatac atcatggaaa ccgaactgtt tgtagatgcg aaccaacaat tcattgaacg
    19381 cacgagcctc gctgacgatt tgatcttcgg tacaaaaaat atgcgcatca tcctgcacaa
    19441 aaccgcgaac cctcatcaga ccgtgcagtg cgccgctcgg ctcgttgcgg tggcaagaac
    19501 cgaattccgc caaacgcatc ggcaaatctc gatatgaacg caaaccgttg ttgaaaattt
    19561 gaacatgacc cggacagttc atcggtttaa ccgcatattc gcgtttttcc gaactggtta
    19621 cgaacatatt gtctttgtag ttgtcccagt ggccggattt ttcccaaaag gttttatcca
    19681 taatttgagg cgttttgacc tctttataac cggcggcgtt cagctcttta cgcatatgct
    19741 gctcgatagt ctgccacaaa gcccaacctt tgggatgcca aaacaccata cccggcgctt
    19801 cgtcctgcaa gtggaacaaa tccagttgct tgcccaattt gcggtggtca cgtttttctg
    19861 cttcttcaat gcgttggata tagtctttta attcgtcttt cgtcgcccaa gcagtgccat
    19921 aaatacgttg cagcatttca ttattgctgt cgccgcgcca atatgcgcct gccagcttgg
    19981 tcagcttgaa gtttttcagg aagcgggtgt tcggaacgtg cggaccgcgg cacatatcga
    20041 catattcctg gtgatgatat atccccatcg cttccacttc gggcatatct tcaatcaggc
    20101 gcagtttgta ttcttcgccg cgctcttgaa aaattttaat cgcctccgca cgcggagtca
    20161 tgattttgac cacatcataa tcttgggcaa tcaattcttt catacgcgct tcaatggcgg
    20221 caacatcttc cggtgtaaac ggtttttccg tggcgatgtc gtaataaaag ccctcttcaa
    20281 tgacggggcc gataaccatt tttgcattag gatagagttg cttgacggca tgcccgacaa
    20341 gatgtgcgca ggaatggcgg atgatttcga tgccttcctg atctttcgga gtaatgattt
    20401 gaacagcaga atcctcaaca atcgggtcgc acgcatcgac caatttgccg tttaccttgc
    20461 ctgccaccgc cgccttcgcc aaaccggcac cgatagacgc agcaatttga gccacggtaa
    20521 cgggggattc gtattggcgg actgaacagt ccggcaaggt aatattcaac atcaaacgct
    20581 ccgaaagatt aaaaaataac ggcataaatg ccgttatggg aatttggtag gcacgattgg
    20641 attcgaacca acgaccccca ccatgtcaag gtggtgctct aaccaactga gctacgtgcc
    20701 ttcaaagaat ttttgtattt tatcgggtcg ttttaatttt tgcaagagag atgtgcattt
    20761 ctttatgcaa cattgtggca agatttgggg ataccgtctg aaaaaccgtg ggaattcatt
    20821 tataatgccg tcttttaaaa tatatttcga cccctgtcgc ccaacttaca ttcaatctta
    20881 tgcttaaatt tactttacac aaaaaagacg gtctcgcccg acgcggcacg ctggagttga
    20941 accacggaaa aatcgaaacg ccggttttca tgccggtcgg tacttacggt tcggtcaaag
    21001 cgatgaaccc gcaaaacctg cacgacatca aggcgcaaat tattttgggc aacacttatc
    21061 atttgtggct gcgtccgggt ttggaggtcg tcgaacagtt tggagggctt cacggtttta
    21121 tcggctggga caaaccgatt ctgaccgact cgggcggttt tcaggttttt tctttgtcgg
    21181 atatgcgcaa gctgaccgaa gaaggctgta cgtttaaaag cccgattaac ggcgacaagc
    21241 tgtttttatc acctgaaatt tccatgaaaa tccaaacggt tttgaactcg gatatcgcga
    21301 tgcagttgga cgaatgcacg ccgggcgaaa caacgcgcga acaggcgcga aaatccctgc
    21361 aaatgagctt gcgctgggcg gaacggagca aaaaagcctt tgaagatttg aaaaacccga
    21421 acgcgctgtt cggcatcgtg caaggcgcga tgtatgagga tttgcgcgaa gaatctttgc
    21481 gcggtttgga ggaatttgat tttcccggcc ttgccgtcgg cggtttgtcc gtcggcgaac
    21541 ccaagcccga aatgtaccgt atgctgcacg ccgtcggccc gattttgccg gaacacaagc
    21601 cgcattacct gatgggcgta ggcacgccgg aagacctcgt gtacggcgtg gcgcacggta
    21661 tcgatatgtt cgactgcgtg atgcccaccc gcaatgcgcg caacggctgg ctgtttaccc
    21721 gtttcggcga tttgaaaatc aaaaacgcca aacacaagct cgacaagcgt ccgatagacg
    21781 aaagctgcac ctgctacgcc tgccaaaatt tcagccgcgc ctacctgcac catctgcacc
    21841 gcgccggcga aatcttgggc gcgcaattga acaccatcca caacctgcat ttctaccaag
    21901 tcattatggc ggaaatgcgc gaagccgtcg aacaaggcaa atttgccgac tggcaggcgc
    21961 aattccacga aaaccgtgcg cgcggcgtgg actgattccc ggcaatgccg tctgaagccg
    22021 cttcagacgg cattttgccg ccttacccta cctgcactat ttttcagaaa ccgatcatgc
    22081 ttccattcct tccagaaccc tccctttcct atacgcaaca aaaccgtacc gccgttttgc
    22141 tgttgaacct cggcacaccc gacgcaccga ccgcacaggc ggtacgccct tatctgaaat
    22201 cctttctgac cgaccggcgc atcgtcgaac tgcccaaatg gctgtggtat cccattctgc
    22261 atggcctggt actgacgttc cgccccaaaa aaagcgcgca cgcctatgaa aaaatctggt
    22321 ttaaggaagg ttcgccccta gaagtttaca cagcacgcca agctgccgcg cttgccgaac
    22381 gtatgcccga cctcatcgtc cgccacgcca tgacttacgg caatccttcg atagcggatg
    22441 tattggcgga gctgaaatcc caaggtgtag gcagattgct tgcgattccc ctgtatccgc
    22501 aatatgccgc ttccagcagc ggagcggcgg tagataaagt gtgcgaacaa ctgctgctgc
    22561 aacgcaacca aatgagcgtc cgcaccattt cgcgctttta tgatgatgcc ggctacattg
    22621 atgcgatgaa aaaccacatc ctccgatatt gggcggaaca cgggcgtggg aaaaaactga
    22681 tgttgagttt tcacggcgta ccgcagaagc actacgacct cggcgacccc tatcccgacg
    22741 agtgccgcca caccgccaaa ctgcttgccg aagcactgga actgaccgaa gacgaatata
    22801 ccgtatcgtt ccaaagccaa ttcggcaggg caaaatgggt tacaccgagc acgcaggatt
    22861 tgttcggcaa actgcccaaa cagggggtaa ccgagctgga cgtattctgc ccgggctttt
    22921 tggcagactg cttggaaacc atggaagaaa tcgccctgac ggggcgggaa cagttttatg
    22981 aagcgggcgg aaaaaactac cgctacatcc cctgcctcaa cgacaacccc gactggatag
    23041 acgcactcgt cgcacttgcc gaagaaaacc ttggcggctg gcgttgaaac tgtttgagcc
    23101 atgtctaaaa tgccgtctga acgccgtgtt cagacggcat tttcccgcta ctttagcgta
    23161 tgatggcatc tatgccggac agcacatcaa gtgcaacctt actaccgatt cggcaaaaaa
    23221 acctttcacc cgctacgcaa aatcaacaac aaggtttcaa acattaagga aacgccaatg
    23281 aacacaaccc gactgccgac cgccttcatc ttgtgctgcc tctgcgccgc cgcttctgcc
    23341 gccgacaaca gcaccatgac aaaagggcaa aaagtgtacg aatccaactg cgtcgcctgc
    23401 cacggcaaga aaggggaagg gcgcggcact gcgtttcctc cgcttttccg gtcggactat
    23461 attatgaaca aaccgcacgt cctgctgcac agcatggtca aaggcatcaa cggtacaatc
    23521 aaagtcaacg gcaaaaccta caacggattc atgcccgcaa ccgccatcag cgatgcggac
    23581 attgccgccg tcgccactta tatcatgaac gcctttgaca acggcggcgg aagcgttacc
    23641 gaaaaagacg taaaacaggc aaaaggcaaa aaaaaccaaa cagacaaaat gccgtccgaa
    23701 accggcaatc cggcttcaga cggcattcaa atcaaacctt tctaacgcgg tactttcagc
    23761 cccatagacc cgatattgcc gccaccgtca acctccttcg cggtcagcat aaagccgccg
    23821 atgtcaatac ggtcgccctc gaccggctcc tgactgtcgg aacgcttatc gaaaagctcg
    23881 gcaaggctca aaccctcttc gcccgcttcc agcttcaaac cgtaagcaag tgccaaatcg
    23941 cccgaacgtg ctgccggcga aacgacaaac tcaccgaaga aatcaaaatt ttcacggacg
    24001 ccgatacccg tctcggtaaa gtatttcgcc attttatcga ccttgccgtc gggcaggatg
    24061 taccacgcca aatcctccgc ctgaagcacg gtgtcggact gcatttcgat tcggctgccg
    24121 ttgcgtatca atgcgaaaca gcgtgcgtca aacgaatcgg aaatcggttc gaccgtatcg
    24181 ggatggtgtc cctccgcttc agattcggca accactttaa acgccgacat tctgacaatt
    24241 tccttttctg ccagccaaat atcatgcgta tctttcggtt cgggcttgtt gggcatggca
    24301 accttcaaca gccgcgccat cacaggaatc gtcgttccct gaatcagcag cgacagcacc
    24361 accacggcaa acgccacatc aaacagcagg tgcgaattgg gaacgcccat caccagcggc
    24421 atcatcgcca gcgaaatcgg tacggctcct cgcaagccca accaactgat atacgccttt
    24481 tcacgcaggc tgtaattgaa tttccacaaa ccgccgaaca ctgccagcgg acgcgcgacc
    24541 agcatcagga acgccgcaat cgccaaggct tccgccgccc tgtccaacac gccggcggga
    24601 gaaaccagca gaccgagcat gacgaacaaa gttgcctgcg ccagccaagc caaaccgtcc
    24661 atcacacgca aaacgtgttc cgtcgcacgg ttgcgctggt taccgacaat gatgccggca
    24721 aggtaaaccg ccaaaaagcc gctgccgcct atggtattgg taaacgcaaa cacaagcagc
    24781 ccgcccgaca caatcatcag cgcgtacaga ccttccgcaa gattcagacg gcgtaccagc
    24841 tttgccaata tctttccgcc cgcccaaccc gtcagcagac cgaaaccgat ttgcagcgca
    24901 agcatccgga caaacgctgc cgcacccgat tccgccggct gcataatcat ggtaatcagt
    24961 gccgtaacca aaaaaaccgc catcgggtcg ttcgcacccg attcgatttc caaagtcgcc
    25021 tgcacccgtt cgttcaaacg cacgccgctg ttgcgcaaaa ggctgaatac cgcgccggca
    25081 tcggtcgaac cgacaatcgc cgccatcagc acgccgaact tccaatccaa accgagataa
    25141 aaagttgcaa acagtcccag cggaagcaca gtggcaaaca cgccccacga agcgagtacc
    25201 gacgcgggct tcaacgcaat ccgaaaactg gaaagctgcg tccgcaaacc gccgtcgagc
    25261 agaataaccg ccaaagcaag ctggctgatg aaattcgcca tcacgacatt attgaaggca
    25321 atgccgccaa cgccttcctc gcccgccagc atgccgacgg caagaaacac cagcagcaaa
    25381 ggcattccca aacgtgcgga caaagtggtc gaaaccacgc tcaggaacaa cagtgcgcca
    25441 cccagcagaa acaggctgtt catccaatcc acgccaacgg ctccccctta aaattcatga
    25501 aattagaatc gaaagattgt aacacggcat tttcttctca cgaaacttta tttcatgtcc
    25561 gccaccgcct tcagacggca ttttcctgtt tcatgccgcc tgataccgcc caaacccgcg
    25621 ttctcctata taatgtcgga ctttgcgccg catttcagga ttttttatgg catttgcctc
    25681 actatttact ctgccggact acatcaccgc cgttttggac gatgttgccc tgatgaccaa
    25741 agccgccgca aagaaaaccg ccggcgtggc cggcgacgat ttggcaccca atgccaaccg
    25801 ggttacaggc gtatcggcag aacgcgagtt gccgattgtt tggccggtgg cgaaaggttc
    25861 ttttgccaac aagccggttt tggtgtctgc ggctctgctg ctttccgcct tcctgccgca
    25921 actcattacg ccgcttttga cggcaggcgg gatttatttg tgctttgagg gcgtggaaaa
    25981 gctgctgcac aagtttctgc accgacatga cgcgcacgat gacggcggcg aaacggcggt
    26041 taacgagcag gctaaaatca aaggtgcaat ccgtaccgac tttatcctgt ccgcagaaat
    26101 catcatcatc gcgctgtgcg tggtcgaaca atacagcctg atgacgcgct cgctcgtgat
    26161 ggcggcaatc ggcatcggca tgactgtttt ggtgtacgga atcgtcgccg tcatcgtcaa
    26221 actcgacgac ttgggcatgc tgctgatgcg ccgcccacaa cctcggcctt ttgcacgatt
    26281 tcctgcacgc gcaacactgg aacgctggct ggacagagta ttccgccaac ctcgttgtcg
    26341 ggccgctttc cggttcgatt gcctgcgccc cgccttgtcg ctgatgaatc gtttcggcag
    26401 gcattgattc ctttttcaaa taccgatgcc gtctgaaaga cgttcagacg gcatattccg
    26461 aacagacaga cgaatatgtt ttccaaactg gacaaatact ggcagcaccc cgccctctac
    26521 tggcctttgc tcatcctttt tgccgccgcc acccccttcg ccttcgcacc ctactaccac
    26581 ttttggctga tgcccttgat tttcggcgcc ttcgtccgcc tcatcgaact gcgtccgcgt
    26641 tttgccgcct cttccgccta cctgttcggt ctgaccgcat acacggcaca gttctactgg
    26701 atacacactg ccctgcacga cgtttccggc ctgcccgacc tctatgccgt accgctgacc
    26761 ttcctgctgc ccgcctacct tgccctttat ccggcactgt gtttctggct gtggaaaaaa
    26821 tttaccctgc cccagggcat aaagaccggt ctggtactgc ccatcctgtg gacgctgacc
    26881 gagtttgccc gcgagcgttt cctgaccgga ttcggctggg gcgcaatcgg ctactcccaa
    26941 atcaccccgg acagcccgct ggcgggtttc gcccccttgg gcggcatcca catggttaca
    27001 ctggcaaccg cctttctcgg tgtctggctg gttttggcga tcgacaacac cgcacgttcg
    27061 ggcaaacgcc tgcttccggc cattctgatt gccgccctgc ttgccgcagg ctacaccgcc
    27121 caacaaaccg acttcacccg ccccgacggc agccgcagca ccgtcgccct gcttcagggc
    27181 aacatcgccc aaaccctcaa atggcgtgaa gaccaagtca tcccgaccat acagaaatat
    27241 tacgaacagg tcggcaaaac cactgccgac atcgtcatcc tgcccgaaac cgccattccc
    27301 atcatgcgcc aaaacctgcc ggaaaacata ctggcgcaat tcgccgaaca ggcgcaaaac
    27361 aacggcagcg cgctcgccgt cggcatcagc caatacactt cggacggcaa cggttacgaa
    27421 aacgccgtca tcaacctgac cggctatcag gaaaacaatc aggacggcat cccttactac
    27481 gccaaaaacc acctcgtccc cttcggcgaa tacaaaccgc tgcccctcct gaccacaccg
    27541 ctttacaaaa tgatggacat gcccctttcc gacttccgca aaggcggcgg caagcaatcc
    27601 gccatgatga tgaaaaacca aaaaatcgcc ttcaacatct gctacgaaga cggattcggc
    27661 gacgaactga ttgccgccgc caaagacgcc acactgcttg ccaatgtcag caatatggcg
    27721 tggtatggaa aatccaacgc catgtaccag cacctccaac aatcgcaggc gcgggctatg
    27781 gaactcggac gctatatggt ccgcgccacc aacaccggcg caaccgccat catctccccc
    27841 aaaggcaaca tcatcgccca agcccaaccc gatacggaaa ccgtattaga aggacacatc
    27901 aaaggctaca tcggcgaaac gccctatatg aaaaccggca gttcatggtg gttgatgggc
    27961 atattgaccc tagccgcact gattcttttc atcttccgaa acaaagaaca ctgatacctg
    28021 cacagtccgt ccggacgggc tgtctttttt caaaccggcc gtttacatac aaccacgtat
    28081 gtaatataat tctaaaatcg aattgagcta tcaggtttac aaaatcaatc aaacttgcct
    28141 tacaaaattt tcctgtcttt caaaccttag cgtatatctc ctgccaaaca tacaaaatac
    28201 aagccaagca gtgatatact gcatcccaac acaccaaacc gccgatacgc agtttcagcc
    28261 gaaaggaaat gccccaaatg aataacgctt tcgcattacc cgccatccaa agcggcaacg
    28321 gcagcctcga acaatacatc cacaccgtca acagcatccc gatgctgtcc caggaagaag
    28381 aaacccgcct cgccgaacgc cggataaagg gcgacctcaa cgccgccaaa caactcatcc
    28441 tgtcccacct gcgcgtcgtc gtttccatcg cgcgcggcta tgacggctac gggctgaatc
    28501 aggccgacct gattcaggaa ggaaatatcg gtctgatgaa ggcggtcaaa cgctacgagc
    28561 ccggtcgagg tgcacgcctg ttttcatttg ccgtgcattg gattaaagcg gaaattcacg
    28621 aattcatcct gaggaactgg cgccttgtcc gcgttgccac caccaaaccg caacgcaagc
    28681 tgtttttcaa cctgcgcagc atgcgtaaaa accttaatgt cctgtctccc aaagaggcac
    28741 aagacatcgc cgacgatttg ggtgtcaaac tttccgaagt tctggaaatg gaacagcgca
    28801 tgacgggaca cgacatcgcc atcatggcag acaacagcga cgacgaggac agtttcgccc
    28861 ccatcgactg gcttgccgac cacgattccg aaccgagccg acaactatcg aaacaggcgc
    28921 attacgccct gcaaaccgaa ggcctgcaaa acgccttggc gcaattggac gacaggagcc
    28981 gccgcattgt ggaaagccgc tggcttcaag acgacggcgg actgacgctg caccagcttg
    29041 ccgccgaata cggcgtatct gccgagcgca tccgccagat tgaagcgaaa gccatgcaga
    29101 aactgcgcgg tttcctgacc gaagaagccg aagcggtttg aaatatacac aataaaatac
    29161 ccgacaaaac aatttgtcgg gtattttatt tggtaaacat caatgcggtt tgtgcgtcgt
    29221 gccgtacacg attttatccg cccaagccat cgcaatcgcc aaaatcaaaa agcagacatg
    29281 caccagacac tgccacatca gttgcttttc cgacagattg gcggaattga taaatgtttg
    29341 gagcaaatgg atggatgaat accgataatc gacatcgaca gccttacctt caataccggt
    29401 gcattcacat ggctcaacca ctcgggccgg tcaggatggt cgtcgatacg caaccgggat
    29461 acgaacgact catacccgcc aatctgcacc atggtcagca aattcgcaat cataaccaca
    29521 tcaatcagat ttaaaacagc gagcatgatg gcgttttcgt ccgacacatc caaattcatg
    29581 accagatgct tcaacgattt caaaaactta taggcacaaa tcgcccgtac aacagtcagc
    29641 cccgcataaa tcggcacttg cagccaacgg cttgcaaaaa tcattttggc aaaaaaccca
    29701 tagcctgttc gggttttgga gattttgctt gatacatttc ccttccttgc agcaacaaaa
    29761 gcgcgtgatt ccaatctacc gctgcgaaat cttcaatttt tcacacccga aaacgcttgc
    29821 cgccatacag atgtgatata ttgattccat tttaacgaac ttcggagaac gacatgagca
    29881 gcggaaatat ccgcatccac agcatgaccg gctttgccaa cgcggcggca gagtgcggca
    29941 gcaaacgcat caacctcgac atccgcgccg tcaaccaccg ctttttggac attcaaatca
    30001 ggatgcccga cgatttgcgc catttggaaa acggcatccg cgagcagatt tcatcccatc
    30061 tggcgcgcgg caaagtcgaa tgcaaaatcc aaattcaaga tgcggaaaac ggcaatcagt
    30121 ctttggaact gaatcgcgtt ctggtcggac agcttgccga aatcaacaaa aacctccgca
    30181 aacattatga tttggcaaaa ctgggcgttg ctgatatttt gcgctttccg ggcgtattgg
    30241 caagccaaaa ggaaaatacg gaagcattgg cgaaaaccat tgtcgaactg gccgacaaag
    30301 cactaaaaga ctttaccgcc gcacgtcggc gtgaagggaa aaaattgggc gaacacctgt
    30361 tacaacgcat taaaaacatg gaagaaatca tcgatgcttt gagcgaaatc ttccccgcgc
    30421 tggtggaagc gcataaagaa aaaatccgca cccgccttgc cgaagccgtc ggcagcatcg
    30481 acaacgacag gctgcaacag gaatttgccc tctttatcca aaaatccgac atcgacgaag
    30541 aattcagccg cctgcgcacc cacattgccg aagtgcgccg tatcgttacc gaacacaaag
    30601 gcagcagcgg caaacggttg gacttcctga tgcaggaact gaaccgcgaa gccaacactt
    30661 tgggcagcaa atccatcgct accgaatgca cccaagcctc ggtcgagctg aaagtcctga
    30721 tcgaacagat gcgcgaacag gtgcagaata tcgaatagcc ctgcctttct ctccaaacat
    30781 gacaatgccg tctgaaactt ttttcagacg gcattcttga tcggctgaaa acctctgttg
    30841 caagcaattc aagattgttc tttcacatcc accaacctcg ttccggcaag gaagtcatac
    30901 agaaactgcc ggtcgggatt cagcagggcg aacccccacg gcaaaatcag ccaaaccaat
    30961 gccgcgccgg ccgcgccctt gggcggaatg ccgaggaagt ggcgcaatcc ggcataagca
    31021 agcataggaa tgaagacgat gaatatgcac gcccaaataa agcgcaggcg cagcaggtgc
    31081 aaagacggct gtatgccgtt aaggtcgcac aagccgattt tccatgtcct catcgccaag
    31141 gtctgcccct gaccgtgcca gttggcgcgg aaataaagcc accatgctcc cattatcagg
    31201 atactcgtta ccaatgcaga aaccgcgata gaaacgggat tcagaaaaat ggcggcaata
    31261 ccggcaagca atgctgccaa acaggtcgcc gcaccgacca gcagcatctc atacagtatg
    31321 gcggcaaagc ggcgtttgag cggggcggtt ttcagtatca tcatcttttc aatcggaata
    31381 tgccgtctga aacgccaaca gacttcagac ggcatttgtg caaagatgcc ttgcggcggt
    31441 taacggtgcg gacgtttctg accgcgcgac tgttttgccg ccatttgatt gatttcctga
    31501 cgtgtcgggc gttttttgaa catagcgtgc gcctcgtcca ccgaacattt ttgcgacagg
    31561 cagatcaggg taatcgcatc tttctggtgt tgtccgccgc tgtggtacat acggcgcagg
    31621 tttgcctcgt tgattctgcc tttttcggga tcgatgtcga attttttcag acgttcgata
    31681 aactggcgtt gctgtcggcg ggatgtccaa acaaagccgc ccgcagcggc ggcgacgatg
    31741 acggcggcaa gcaggatggc aagcggccag gaatcgacaa tcaatacgat aatgaggctt
    31801 aaaacggcgg tggtcagtaa aatcaggccg cccaagcgca gtttggtctt attgtccata
    31861 gggttgatgt tcctttattg aggtggtcgt gccgtctgaa atgttttcgg acggattggt
    31921 gaatactttt caggattaac ggtattttac agtattttcg gattttgggg attaccgctt
    31981 tattccgcca gcgacacgac aagctgtttg aataccgtcc atgcgtcgcc gtcttccgcg
    32041 cctttgatga ttcggtcgat ttgggcgcag gttttgagcg cgtcaagcag gcggacgacg
    32101 gaaatccgct tgaccgcaag cggtacgagc gtctgtttgt cgccccaaag cctgaggctg
    32161 ttgcggacgg attggatgct ctgcccctgt ttcagggcag cggcaagccg aatcagcgtc
    32221 cgcacgtctt cggcaaccgc ccacagaagc agcaccggtt cttcgccttc ttcctccaac
    32281 ccgtccaaca gcctgcatac gcgcgggaca tcgcccttca tccacgcgcc tgccagttgg
    32341 aacgcgtcga agcgggcaac attggcaacg gcggtttgcg cctcatcgat attgacggca
    32401 tgccctttcg gatacagcag cgcgagcttg tcgatttcct gacgtgccgc caacagattg
    32461 ccttccacgc gctcggcaaa cagtgccaat gcgtctgcct cgatacccaa accgattttg
    32521 tccagccgtc cgcgtatcca ttggggcaaa gccgccgcac cgaccggttt ggcttcccat
    32581 acttccccct ttgccgccaa tgcggcaaac catttggact ggagccgggt tttctccagt
    32641 ttgggcagca aaaccagcgt taccgtatct tccggcagtc gggcggcaaa atcctgcaat
    32701 gcctcgccgc cgtttttgcc gggttttccg ttggggatat gcaattccaa cagtttcaaa
    32761 tcggcaaaca gacccgcact gcctgcggtt tgcagcagtt cgttccaatc gaaagacgcg
    32821 tcggcggtat aggcttcgcg gttgagataa ccctgcttct tcgccgccgc cctcaatgcg
    32881 tccactgcct cgatacgcag cagttcttcc tcgccgtgga tgacgtacag aggtttcaaa
    32941 ggcgcgtccg tatcgatgcg tccgatatgt gccgccacgt cattccgcct tcagaaaggt
    33001 caggcggcgg acaatctgtt cggcaacatc ctgccgcatt tccgcccaca gggtttcttc
    33061 ttcttcctgt ttgcccaaaa tttcgttgtc ggcataatcc aaaatgcggc ggacggacac
    33121 ggtcatcggt ttgccgaccg gctcgccgcg tttcaatacc tgcgcttcaa ccgtcaatat
    33181 caaaagatat tcgttgatga ctgccgcacg ggtaacggta taggtttcct tgttttggga
    33241 aacgctgtct atacgcaggg tcatctgcgc gccggcagca tcgtccaccc tgcccgaagc
    33301 ctgatacagc gcggtttcca aaggaaactg caatgcctgt ccgccttcga tgtgccagct
    33361 ccggtaggtc agcggcggag aaatgccgtc tgcacctttc aggtggaaac cgcacgcgcc
    33421 cagcatcaag gctgcggcag taaggaatat tttgttcatc gcttgccttt ctcaaaagca
    33481 taaagtcggt agattatatc agcggcgtgt tcagacggca ttgccgtcca gccaaatcag
    33541 gctcgccata cgccccgtcg ctccgtcgcg gcggtaggaa aagaaaatat cccgttccaa
    33601 aaccgtacaa tgcgtgccgc cgtacacgcc gcccacgcct tcgcgcttca gaatcaggcg
    33661 ggcgagcgcg tagaggtcgg caaggaattt gccgccgccg atgggttcaa atgcagcagc
    33721 ggcttcgggc atggacgtgc agaacgcatc aaacacgtcc tgcccgactt caaacgcatc
    33781 cgcactgatg gcagggccga gatacgccat aatttcgaca ggcggaacct tcatcgcggc
    33841 aatggtgttt tgcaatacgc cgcccgccaa accgctccag cccgcgtgtg cggcggcaac
    33901 cgccgtacct gccctgtcgc aaaatagaac gggcagacag tctgcagtca tcacggcaca
    33961 gacggcttta cccgtgtcgt ccacggaagc gtcggcatcg ggcgtgccgt ccaatgcttc
    34021 ggcagcattg acgacaatgg tgctgtggat ttgattgaga taggcaacgg gcaatccgac
    34081 ctgctgttgc acgatttcgc ggttgcggcg cacggtttca gggtcgtcgc cgacgtgcgt
    34141 accgaggttc aaactctgat acgcaccttg gctcacgccg ccgttgcgcg tggtaatcag
    34201 ggttttcaca ttggcgggcg cgggccaatc ggcggtcagg aagtttttgc ctttcggggc
    34261 gagatttagg gtttctgtga tggttttcat tggatattgg aataggtgtg cggcggaaag
    34321 ggttgattaa ctgccttcgg ggcaaatgga tgcttgtttt ttcgtcattc ccgcacaggc
    34381 gggaattcgg agattcggga ttgttttcaa acgttcgggt ttggattctt gccggtgcgg
    34441 gaatgatgcc cttatatact ttctccgagc tttatatgct tcaacagggg acggcacatc
    34501 aagcaccgca ctgcgtgttg cccgaacgag gctgccctcc gattagattc tatcgctata
    34561 aacagacggg tttcaaccga aaaggaatgg ggataaagtc catttccgac acctctcggg
    34621 cgatgccgtc tgaaaaccaa tctccacttt cagacggcat tgtttaggcg cggtcatttt
    34681 gcaaattaag gttaaggcgt gtatgcttgc cttcccttaa aaacaaacac gcaacgcatt
    34741 attatgaata tcctcagtaa aatcagcagc tttatcggaa aaacattttc cctctgaacc
    34801 tcgctctttg ccgccgccgc ttttttcgcg cccgacacct tcaaatgggc ggggccttat
    34861 attccttggc tgttgggcat tattatgttc ggtatgggtt tgacgctcaa accttccgac
    34921 ttcgatattt tgttcaaaca tcccaaagcc gtcatcatcg gcgtaatcgc acaattcgcc
    34981 attatgccgg caaccgcctg gctgccgtcc aaactgttga acctgcctgc cgaaatcgcg
    35041 gtcggcgtga ttttggtcgg ctgctgcccg ggcggtacgg cttccaatgt gatgacctat
    35101 ctggcgcgcg gcaatgtggc tttgtcggtt gccgttacgt ctgtttccac cctgatttcc
    35161 ccattgctga ctcccgccat cttcctgatg cttgccggtg aaatgctgga aatccaagcg
    35221 gccggtatgt tgatgtccat cgtcaaaatg gttttgctcc ccattgtttt gggtttgatt
    35281 gtccataagg ttttgggcag taaaaccgaa aagccgaccg atgcgctgcc gctggtttcc
    35341 gttgccgcca tcgtgctgat tatcggcgcg gtagtcgggg caagcaaagg caagattatg
    35401 gaaagcggcc tgctgatttt tgcggttgtc gtactccgca acggcatcgg ctacctgctc
    35461 ggcttctttg ccgccaaatg gaccggcctg ccttatgatg cacaaaaaac gctggccatc
    35521 gaagtcggta tgcaaaactc gggcctggcc gccgcgcttg ccgccgcaca ctttgccgcc
    35581 gcgccggtcg ttgccgttcc gggcgcattg ttcagcgtgt ggcacaatat ctccggctcg
    35641 ctgctggcaa cttattgggc ggccaaagcc ggtaaacata aaaaacccta aacgcctttc
    35701 atcataatgc cgtctgaacg gggcaaccga aaccggcggt ttctgcccgg ccccttcaga
    35761 cggcctttgt ttgaaatcgg cggggacgat tgcccggctg ctccggttcg gctgcctgcg
    35821 ttcgccccgc ctttcaagcc gcctcactcc cttacataaa ccacttcgac atcgtagtcg
    35881 tcttcgttcc aatcgtcatc gtcgtccgcg ccgaatttgt cctgccattc ctcttcattg
    35941 ctcaaagacg aatccaaacc ggcttcaaga cggaggacgg acaacaaatg atatatgtcg
    36001 tccggaatcg gtgcctcaaa cgaaacggtt tcgccgcttt ccggatgggt aaaactcaag
    36061 cggtaggcgt gcaacgcctg acgcgcgccc aaacttttaa cggcttcttt caccgtgtcg
    36121 ccgcacggat ggcgcgggtt gccgtaaacc gggtcgccgg caagcggatg gttggcctcg
    36181 cgcatatgga cgcggatttg gtgcgtcctg cccgttccga gcgagcattc gatgtagctg
    36241 tgggcaagat agcgttccaa cactttgacg tgggttacgg caggcttgcc gccgaatttg
    36301 acggctgcca ttttcaggcg gttgtgcgga tcgcgtccga tttgggtttc gattttaccg
    36361 tcgaagggga cgatgccgtt ggcgacggcg cggtagatgc gtttgaccgt gcgttcttga
    36421 agctgcctca cgagggaatt ttgcgccggc agggttttgg caaccaccat cagcccgctg
    36481 gtttctttgt ccaaacggtg tacgatgccc gcgcgcggaa tttgggacag ctcggggcaa
    36541 tgcgccaaca ggccgttgag cagcgtcccc gtccagttgc ccgccgccgg atgcaccacc
    36601 agtccggccg gtttgttgac gacgatgacg gtatcgtctt cgtaaacaat atccaaatcc
    36661 ataggctccg gaacaaacgc cagattttcc tcactcggac ggacggttac acaaatttgc
    36721 tcgccgccta tcattttgtc tttgggttgc gaaggtttat cgtttacaat aaccgcgcct
    36781 tctttaatcc atgatgtcag gcggctgcgc gagtagtcgg gcagaagttt cgccaacacc
    36841 gcatccaacc gcccgccggc aagctcgggc ggaacggtca gaccgatacg gttttcagtt
    36901 ccggaagctg acgcaaagtc tgaatcgtcg ctataatcgg attcattatc aaaggaagta
    36961 ttctgcatga aaaaaattct tttaacggtt tcattaggtt tggcactgag tgcctgtgcc
    37021 actcaaggta cggccgacaa agatgcccag attacccaag attggagtgt ggagaagctc
    37081 tatgccgaag cccaggacga attgaacagc agcaattata cgcgggctgt caagttatac
    37141 gaaatcttgg aatcgcgctt ccccaccagc cgccatgccc ggcaatccca actggatacc
    37201 gcatacgcct attataaaga cgatgaaaaa gacaaggctc tggcggcaat cgaacgcttc
    37261 cgccgcctcc atccgcagca tccgaacatg gattacgcgc tgtacctgcg cggcttggtg
    37321 ctgtttaacg aagatcagtc cttcctgaac aaactggcct cgcaagactg gtccgaccgc
    37381 gacccgaaag ccaaccgcga agcgtaccag gcgtttgcgg aactcgtcca acgcttcccg
    37441 aacagcaaat acgccgccga tgcaaccgca cgcatggtca aactggtcga tgctttgggc
    37501 ggcaatgaaa tgtcggtggc gcgttactac atgaaacgcg gcgcatatat cgccgccgcc
    37561 aaccgcgcca aaaaaattat cggcagctat caaaatacac gctatgtcga agaatcactg
    37621 gccatattgg aacttgccta caagaagttg gacaagcccc agcttgccgc cgatacgcgc
    37681 cgcgtattgg aaaccaactt cccgaaaagc ccgtttttga cgcacgcttg gcagcccgac
    37741 gatatgccct ggtggcgtta ctggcattaa gcccatcctc gaaatgccgt ctgaagccct
    37801 ttcagacggc attttccgtt ccggcaccag ccttcccgtt cactcaaacg gttttttctg
    37861 ccaatagaat ttatacactc tcaaacgttg agcctttacg cctccgcgtc ccaagccgaa
    37921 ttgcagcgcg cccgacaaat ccgtccgcaa caacttgatg ccgtgtgcgc ggacacggtt
    37981 ctgcaccgct tcggtcggat gcttgtaggc gttggcataa ccgcttgaag caacggcata
    38041 ttcgggcgaa acggcattga ggaacacgcc cgacgaggac gtattgctgc cgtgatgccc
    38101 caacaccaac acctggctgt acaggttgcc tccatacttg ccgaccaggc tttcctcgcc
    38161 cttcgtatcc aaatcgcccg ttaccagcag tgccgcaccg cccgccacaa cacgcaaaac
    38221 acaacttttc ccattatcat cgatattttt gcgttcagac ggcctcaaaa actcgaaatc
    38281 cacgccgtcc cattgccaac gctgttccgc acaatgccgc gccccctcat aaaattccgg
    38341 ctgtccggca taaatcccgc cgttcggtat cttgccgact gcctgaaagc cgccgtcgtg
    38401 gtcgctgtcg tgatgcgaca gaaccagctt gtccaaacgg cggacacccg ccgcattcaa
    38461 actcggcacg atacccgttt gtgccgccgc taccgttccc gtatcgaaaa gcagatggcg
    38521 gtttgccgtc cgcacaagca ccgacaagcc ctgccccgca tcccaaaccg taaccgcagc
    38581 ttcgttttca ggtactgctt caggacggta ggatacaaac cctgccaaca gcaacaccgc
    38641 ccacggacgc aaacccaagc cgcgcggcag cagcaacagc aaagcggcac acaccgccaa
    38701 caccagcagc ggcaaaggtg cggcggcaac ggcaaactca ggcgacacat cggcaagcca
    38761 caccaaaaac cgcaaagtgt attccgccaa aaatgccccc gcctgctgaa gcggcgcaaa
    38821 cggcacgacc gaacccagca acgccagcgg ggtcaacacc caagaaaacc acggtatcga
    38881 caccgcattg accaaagggc tgaccaaagg cagcgaggca aacagataac cgagcaaaac
    38941 cagagacaac accgaagccg cccactgccc gcgcacggcg gtttgccgtt tcccctcgta
    39001 caaacgccct gcacacgccc atatcagggc cgccaccaaa ccgaaagaca gccaagtacc
    39061 gacacccaaa accgccaaag ggtcgaacag cagcaccgcc gccaacgcct gccaccacgt
    39121 cgcccacgcc gacagtcttc ccctgcgcca cgcccacgcg aacgccgcca acatcaaaac
    39181 actgcgctgc gtcggcacgg aaaaaccggc aagcagcgcg taaaacagcg cgcccgcaca
    39241 ccccgccgcc aaaacccacg cgcgcggccg ggcgggaagg cgcggggaac aggcaagcag
    39301 ccgcttcgcc agccacgcaa acagcaccgc caccatcgta acgtgcaaac ccgagatact
    39361 gaccaaatgc gtcagcccca acggccggaa cgcctgccac aatccggggc gcaatgccga
    39421 ctgttcgccc acgctcaacg cgcgcatcag cccgatgccg tctgaaagcc cgccgtccgc
    39481 atccgcctgc cgccaattgc ggctgatgcg gctgcgccaa accgcaatcc cccaaccgct
    39541 tccgccatgc agcaaaaccc tgtccgcacc gaccgtcccc acgccgccta ccccgttgga
    39601 taatgcccac gcctcacggt tcaaaccgcg caggttcaac tccccgacga cggggtgcac
    39661 gcgtgccgtt atccgccatc tgctcccgac cgcccattcg cgccgtttgt agtccgacag
    39721 cagcaaatca aacgtccgac cgccgctgtc cacagccttt gccgcaaact gcacgcgccg
    39781 cccgtccgac ctcggcatat ccgtcacttc caccgtcaaa ggcacgcctg aaaccgcctc
    39841 cgcccgccat tgcgaagaca gtgccgcttc cgttctgaat acgccgtaag ccgcgcccgc
    39901 caacacgcac agcatcagac cggcaaacgc aaaacgcctt gcaagcacag ccaaaaccgc
    39961 aaaagccgcc aaccaaaacg gccaatgcgg tacaaccgac agtgcgaacg aaactgccac
    40021 gccgacaacc caacacggca aaaaccaacg acggaacacg tctccccact cccgatataa
    40081 aaaacaaacg atgataaagc aaagccataa aaccgtcata acaagatttg aaaatgataa
    40141 gaatgcaaac gaaaagcctc agtattatcc gagacaacgg caaactttcc atcgtcattc
    40201 ccgcaaaagc gggaatccaa agacaaaaag ccgcagaaat ttatcggaaa gaaccgaacc
    40261 acaaaaaccg gcttcccacc ttcgcttgaa taacagaaca aagaaagcgg aaataaaaac
    40321 atcaggattc ttttttaaat tggcaataaa acaccaaaat gaatgagtta cacaaaaaaa
    40381 tactcaacac cacccaaccg gcgtaaaatg caaaacatta tcgctattaa aacggtaaaa
    40441 ccttatgaaa gccaaacgtt ttaaaattaa cgccatatcc ttatccatct ttcttgccta
    40501 tgcccttacg ccatactcag aagcggcatt ggtgagagac gatgtcgatt atcaaatatt
    40561 ccgtgacttt gcagaaaaca aaggcaaatt ttttgtcggc gcaaccgatt tatcagtgaa
    40621 aaacaaacga ggtcaaaaca tcggcaacgc attatccaac gtaccgatga ttgattttag
    40681 cgttgcagat gtcaacaaac gcatagcaac ggtagtcgat ccccaatatg ccgtcagcgt
    40741 caaacacgcc aaagcagaag tccatacttt ttattacggc caatacaacg gtcataatga
    40801 tgtagccgac aaagaaaatg aataccgcgt tgtcgaacaa aataactatg aaccccataa
    40861 agcttggggt gcgagcaatt taggccgcct cgaagattat aatatggcgc gttttaataa
    40921 gtttgtaacc gaagtggcac cgattgcacc aactgatgcc ggcggcggat tggataccta
    40981 caaagataaa aaccgttttt cctcttttgt cagaatcggt gcgggcaggc aattagttta
    41041 tgaaaaaggg gtttatcatc aagaaggaaa tgaaaaaggc tacgatttgc gcgatctttc
    41101 acaagcctat cgttatgcca ttgcaggtac gccttataaa gatattaata ttgaccaaac
    41161 aatgaatacc gaaggtttga tcggctttgg taatcataat aaacagtatt cggcagaaga
    41221 acttaaacaa gcactttcgc aagatgcgtt aacaaattac ggcgtgttgg gcgatagcgg
    41281 ctctccgtta tttgcttttg acaaacaaaa aaatcaatgg gtctttttgg gaacttacga
    41341 ttattgggca ggttacggaa aaaaatcatg gcaagaatgg aatatctata aaaaagaatt
    41401 tgcagataaa atcaaacaac acgataacgc cggcaccgtc aaaggtaatg gagaacatca
    41461 ttggaaaacc acgggtacaa acagccatat cggttcgaca gcggtaaggc ttgccaacaa
    41521 tgaaggagat gcgaacaacg gacaaaatgt tacctttgaa gacaacggca ctttggtatt
    41581 ggatcaaaac atcaaccaag gcgcgggcgg cctgtttttc aaaggcgatt acacagtcaa
    41641 aggcgcaaat aatgacatca cttggttagg tgcggggatt gatgttgccg acggcaaaaa
    41701 agtcgtttgg caagtcaaaa atccgaatgg cgacagattg gcaaaaatcg gcaaaggcac
    41761 tttggaaata aacggcacag gcgttaacca agggcaatta aaggtcggcg acggtacggt
    41821 tattctgaat caaaaagccg atgccgacaa aaaagttcag gctttctccc aagtcggcat
    41881 tgtcagcgga cgcggtacat tggtattaaa tagttcaaat cagattaatc ccgataacct
    41941 atatttcggt ttccgtggcg gtcgtttgga tgccaatggc aatgacttga cttttgaaca
    42001 catccgcaac gtggatgaag gcgcgcgcat tgtcaaccac aacacagacc acgcctccac
    42061 aatcacacta acgggtaaat ctttaattac caatccgaac agcctttcgg tacatagcat
    42121 acaaaatgac tatgatgaag atgattatag ttattattac cgaccaaggc gacccatccc
    42181 acaaggcaaa gatctttatt acaaaaacta tcgttattac gccctaaaat ccggcggcag
    42241 cgtgaacgca ccgatgcccg agaacggtgt agcagaaaat aacgactggg tctttatggg
    42301 atatacgcaa gaagaagcca agaaaaacgc gatgaaccac aaaaacaatc agcgtattag
    42361 cggttttagc ggtttctttg gcgaagaaaa cggaaaagga cataacggcg cattaaacct
    42421 taatttcaat ggcaaaagcg cgcaaaaccg tttcttgtta acaggcggcg ccaatttaaa
    42481 cggaaaaata agcgtaactc aaggcaatgt cttgttatca ggtcgtccaa caccacacgc
    42541 aagagatttt gtgaacaaat cttcagcccg aaaagacgca catttctcca aaaacaatga
    42601 agtcgtattt gaagacgact ggataaaccg cacattcaaa gccgcagaaa ttgcggttaa
    42661 ccaatccgca tcattctctt ccggaagaaa tgtatccgac atcaccgcca acatcaccgc
    42721 aacagacaac gccaaagtaa atttgggtta caaaaacggc gatgaggttt gcgtgcgctc
    42781 ggactatacc ggttacgtta cctgcaacac aggcaactta tccgataagg ctttaaacag
    42841 ctttgatgcg acacggatta acggcaatgt gaatttgaat cagaatgcgg cattggtctt
    42901 gggcaaggct gcattatggg ggaaaattca agggcaagga aacagccgtg tcagcctaaa
    42961 ccaacatagc aaatggcatt tgaccggcga cagccaagta cacaatctgt cattggcgga
    43021 tagccatatt catttgaaca acgcttccga tgcgcaaagt gcaaataaat accacacgat
    43081 caaaatcaat catttatccg gtaacgggca ttttcattat ctgacggact tggcgaaaaa
    43141 tcttggggat aaagtgcttg tgaaggaatc cgcatccggc cattatcagc tccatgttca
    43201 aaataaaaca ggcgaaccta atcaggaagg gctggatctc tttgatgcat catccgtaca
    43261 agaccgctcc cgcctttttg tttccttggc aaatcattac gtcgatttag gcgcattgcg
    43321 ttatacaatc aaaacagaaa acggtattac ccggttgtac aatccttatg ccgggaaccg
    43381 ccgcccagtc aagccggccc cgtctcctgc cgcaaacacg gcttctcaag cacaaaaggc
    43441 aacacaaacg gacggtgcac aaattgccaa acctcaagat attgtcgtcg caccgcccag
    43501 cccgcaggca aatcaagccg aagaagcaaa acgccaacaa gcaaaagcgg agcaagtgaa
    43561 gcgtcagcaa gcagaagcag ggcgtaaatc ggcagagttg tcggcaaaac aaagggccgg
    43621 ggaagaggaa c
//
LOCUS       DAXDXW010000004        42004 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXDXW010000004 DAXDXW010000000
VERSION     DAXDXW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 42004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42004
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.004"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..1605
                     /gene="trbL"
                     /locus_tag="ACFB64_000162"
     CDS             <1..1605
                     /gene="trbL"
                     /locus_tag="ACFB64_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HFC1208619.1"
                     /translation="KKKYIYYFIFFITLSMPIISHAAGQGGVLDEALSRYEGKIGAWE
                     AAFYKAGLFIFWSLSSISIVMTGAQLIFQRDNISSFFAEFTRLILFLGFFLWIITNGI
                     KITTSIKDGLMKLAGQASRNGEQITPSSIVDIGFNLFDKIVENSSIWDPIDSATLIVI
                     GLVILLILAAVGINMLLMLITTYIVIYSGIIILGFGGGRWTSDMAVGYLKQVLNLSLQ
                     LAAMILIIGIGQSIVQDTINTIGTPGFRELAVVLVQTALLVGLVVKIPPMIGSLAGGA
                     GSGGIGSFGVGNAIAAAAMLAGAAAGAGAALKSLAVEAGGLKKAFEAAKAGLGGSNGS
                     SDSGSGKMDSAVDAVAGANAGDKSNQDGKPLTAESSGNSENGSEGSTTGNSGGNPSGS
                     SEENTISSGSSSSSGEANSTNTSASSGAEGSAEGEATSSETSQSASDSPSNNSGEATQ
                     TADTTPKTTNTGGGSPLATAMGGNKVSNWQAAKNVAVGLVSSKWNNAVNNSAGGRLVK
                     HWEDEKKKTEDPTKNPQKNPEGDDNA"
     gene            1598..2170
                     /locus_tag="ACFB64_000163"
     CDS             1598..2170
                     /locus_tag="ACFB64_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFC1208620.1"
                     /translation="MLKKTLICILLVGSVHFAMAESLNDALNRSRSGNSNNNRPQVED
                     LFTGDVKLACEATLCLSTGNRPSECQPAIHRYFSIRHKRMGDTIRARHNFLRLCPASK
                     EQGMLELVNAIANGAGRCDARELNRINRSTYIKRVWVGSRRHGDWQEVKVPYIRNAKP
                     SYCSAYFENGWTQLGTRYVGEEKNGGKWVD"
     gene            2170..2769
                     /locus_tag="ACFB64_000164"
     CDS             2170..2769
                     /locus_tag="ACFB64_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbN"
                     /protein_id="HFC1208621.1"
                     /translation="MVYVADLPPFMQERVTCSIAAAEKYHLPPHYLLAVAERENGRPG
                     QQVRNTNGTYDVGAMQINTAYIRTLSRYGVTQSAVAQPGCYAYYLAAWRIKRHIVYDR
                     KGDIWTKIANYHSYTPKYNAIYRAAIIRLAEKWERWLAGRQGNNAKGNVPTQAVPAVR
                     TIRSQPLPKQYVSPTTVYVPRTIRVVERSPEPEPETVQN"
     gene            2878..3162
                     /locus_tag="ACFB64_000165"
     CDS             2878..3162
                     /locus_tag="ACFB64_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HFC1208622.1"
                     /translation="MAYAIAFDLDTTVLQELYPNSSWQNAYGDVKRTLVGLGFNHQQG
                     SVYFGNSEITAVSCVLAAQKLSQTYAWFKPAVSDIRMLRIEEMNDLQPAL"
     gene            complement(3272..3826)
                     /locus_tag="ACFB64_000166"
     CDS             complement(3272..3826)
                     /locus_tag="ACFB64_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFC1208623.1"
                     /translation="MIIGYLRVSTKEQNVAAQRQVIQDKYQIERWYEDQAASGAVKAL
                     NRPEFRKMAEFVRQGDTVVVVAIDRLGRNTIDVLETVQFLQKKGVAVTSLRENFDLAS
                     PLGTAMLAIVSAFAELERSYIKERQMAGIRNAREEGKKLGRKVKVDPEEIRRWRQSEQ
                     TSISRTAAHFNVSESTVKRACKAK"
     gene            complement(3878..4309)
                     /locus_tag="ACFB64_000167"
     CDS             complement(3878..4309)
                     /locus_tag="ACFB64_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFC1208624.1"
                     /translation="MKEKMRLITEISLIIRELSYSRRGYRPESYAAALDNLTIIGDLA
                     DILHNIEAATGNPFVQRMVDEKLRKFVTDFPAYKVRLSPFLNGCPHDLSLGAESLGKG
                     ESIADALAVPPEEVDIADIEFDRVLIEDDRWTGAYDGGNAQ"
     gene            complement(4324..5529)
                     /locus_tag="ACFB64_000168"
     CDS             complement(4324..5529)
                     /locus_tag="ACFB64_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFC1208625.1"
                     /translation="MVKLSSDINLRDFGNNEYLSSVQDEAIRFATEQTDEILSLYSQH
                     ADTEGGRYVCADTFKELFPAFENKEDRATVNNAIHNSAAVLSSTQFDEVLKRDEPQKK
                     EVIFVTGIPGSGKTSTVKNMMMQDTTKLLFEGQLARPQSAFRKIEQCLERNLEVTIVA
                     VSMRAERASDNTYKRFNEYGRGASIGIMADIQANLPDGLKQIRDKFGDAVKIVGINQD
                     RNSEFIDKFDDVIKMLSLGSQEQILGRLAEKIQSDFDSGKISRECFNQAKGSMDLESV
                     FAKKEYSQQRVVTNSKGVTLETKSANELWSKVEQIPVTGMKAGIYLLGQAKKAETGQT
                     YSGEIIYKDAAAVFQKTKNGLVRHNATHNEERLAKLVEIGQNVSIGSNKGKLIVKSLE
                     YSAKKSISR"
     gene            complement(5536..5721)
                     /locus_tag="ACFB64_000169"
     CDS             complement(5536..5721)
                     /locus_tag="ACFB64_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208626.1"
                     /translation="MNKVEPQESNAIRMIKEACEKNRRMMTDEAFRKEVEKRLYAGPS
                     PELLAKLRVLWAANKEQ"
     gene            complement(5755..6963)
                     /locus_tag="ACFB64_000170"
     CDS             complement(5755..6963)
                     /locus_tag="ACFB64_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFC1208627.1"
                     /translation="MIEYTPLSAEGKARILNGFMKPRLSRTQSVEQPKIVLVGAQPGA
                     GKSKAASLAKSELRQEGGYIHVDADIMRALIPAPEGVVYSSEQTQKDAGALAISVRNS
                     AKENRRNIVEEGTFRNAASISQFIRDRKSEGYGVEMLAVATASEESVAGIFKRYEEQH
                     AKGVSQPRFVEESYHNEAMAGFKDTLSQCESSFDRVRVTNRAGDILYDSLNRRQNQHE
                     TAKDALSAYQEITPKRLKQVVKAWDEIQLQAESRSIDPIPNYLGMVKQHSEAIYQRVE
                     EIYRQERVVANSEGATLQRKSGDTWQDIEKAEAKGMKAGIHMLGTAKPAKSGREYSGE
                     IVHKDEASVFQKTDQGLIRHKAVQGMAEGKFSSLSEQVEIGQKVSIKREGNELSVKPA
                     DASLKKTMKR"
     gene            complement(6960..7214)
                     /locus_tag="ACFB64_000171"
     CDS             complement(6960..7214)
                     /locus_tag="ACFB64_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208628.1"
                     /translation="MQMQETIPGINYEVALELLGDSMRPLIQARESEFVKDKPNQAFI
                     EYCTARLEAIDMLRDSLSPEDTDIIARIFNPDDQTVGRVK"
     gene            complement(7591..8112)
                     /locus_tag="ACFB64_000172"
     CDS             complement(7591..8112)
                     /locus_tag="ACFB64_000172"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208629.1"
                     /translation="MNMKTVQEYEIIIRLTKENGRYGAAAETVSGGLLESVPPLSAAG
                     NDALYALLHALALKNNFSGCLKEAQRRESVKNIEAKIEEKMKPIELQYYFSDMNVGEF
                     NAYLTVKNLAKPTFISHIDIDEDDKMFIANLAKGIASEYKSLFYLDVNRQTIEKALLR
                     YLEVCVSCNSAQD"
     gene            8500..8616
                     /locus_tag="ACFB64_000173"
     CDS             8500..8616
                     /locus_tag="ACFB64_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HFC1208630.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            8632..10551
                     /gene="tet(M)"
                     /locus_tag="ACFB64_000174"
     CDS             8632..10551
                     /gene="tet(M)"
                     /locus_tag="ACFB64_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFC1208631.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDPVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQKYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            10643..11116
                     /locus_tag="ACFB64_000175"
     CDS             10643..11116
                     /locus_tag="ACFB64_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208632.1"
                     /translation="MPHEQMIDAFSAVIQNSLSLYEEQTPIYYYLTICGSWVLTALGI
                     LSLVSVFFTIKQKKPYRFIVPILFLWLGYESIHMPRPNLAVFYGYNLAQLSYPANENP
                     NSFLHLADGSNPICCFIGEDEYLQAKRLGFKEYADAKAKALVDLVEMEKKSKQSD"
     gene            complement(11268..12164)
                     /locus_tag="ACFB64_000176"
     CDS             complement(11268..12164)
                     /locus_tag="ACFB64_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine modification methylase"
                     /protein_id="HFC1208633.1"
                     /translation="MFTHSVCSYPQRGNYGKSGYRGNCTGHIIRDFVESYLAPEGLFA
                     DPSIGSGTSSDVAEEMGIRFKGTDLHQGYNLLTQDFAAFLGEQANLIWWHPPYWNMIR
                     YSGNQWGDAANPWDLSQMDLEQFQEAMLLAMMNIHDATEQGGHYGILMGNMRKDGRYY
                     NLSSMVERMAAGRLVDEIIKIQHNCVSDSRQYGGKLVRIAHEKLLVFKKEIQTALYFL
                     AKAMNRVEAVTAVTWKAAVRRVLQAADGRNMGLTEIYGEMEPYAAARAENRNWQAKVR
                     QVLQDERFFLRVGKGIYALKTE"
     gene            complement(12168..12509)
                     /locus_tag="ACFB64_000177"
     CDS             complement(12168..12509)
                     /locus_tag="ACFB64_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208634.1"
                     /translation="MLYWDDVSDSFDGWMNLLRQLGGDSFINFEQDIWETARTYETVP
                     HFGNLRQHHLLERLQDTIRNRWPFLRTGFLINALDTHFYVNDEPVETMRDLDAVLGCH
                     YRENEDDLKEE"
     gene            complement(12523..12837)
                     /locus_tag="ACFB64_000178"
     CDS             complement(12523..12837)
                     /locus_tag="ACFB64_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208635.1"
                     /translation="MASYTNRLTGHPNVFVEQNIWSNGELMGFSPINVMWDGRNAPTL
                     LCRYTFDGGQYYSLQVSEAAELETRGYQIVCDDLQCLKLKTAKARRSGILALILADAG
                     IE"
     gene            complement(13204..13563)
                     /locus_tag="ACFB64_000179"
     CDS             complement(13204..13563)
                     /locus_tag="ACFB64_000179"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208636.1"
                     /translation="MSLSAENRTFYLVETGEIGFGSSALFISQSLEEAEAVVADFFKG
                     DEKLHYETDGNQPEISLGMGEPQEEEYIPFLPREPFFWTKGGFGMMGTEYVTIRKTIN
                     GYLEGAGSFRLIKSQQD"
     gene            complement(13560..13994)
                     /locus_tag="ACFB64_000180"
     CDS             complement(13560..13994)
                     /locus_tag="ACFB64_000180"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HFC1208637.1"
                     /translation="MSIYRPASRERKNIKFFADGQYIQEGYKALCWMMRDVVDNHQMH
                     AININLINLLFAQQQYLRDLGRPNPELVLHSGFRTRRHNDSLEGAAKNSQHLSGNAGD
                     FHIERASLSELAALARRFRVGGIGIYPTFIHNDIGVYREWRK"
     gene            complement(14067..14120)
                     /locus_tag="ACFB64_000181"
                     /pseudo
     CDS             complement(14067..14120)
                     /locus_tag="ACFB64_000181"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
     gene            complement(14117..14587)
                     /locus_tag="ACFB64_000182"
     CDS             complement(14117..14587)
                     /locus_tag="ACFB64_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor"
                     /protein_id="HFC1208638.1"
                     /translation="MAFFLTYHKQAREGWFWRVFGMAFDGRLRQTLQNIAVDYRKTSQ
                     NQPLAFDDYLEMLQRHEEEKMGVSAERNQSEQAAVKAFQKQYGLNHRVDTHADTKTLS
                     ITITATDEDFIPAVEMGLEQVLGSLAAGETANEGGNEYGRYAFMVSGNVKGRER"
     gene            complement(14620..17619)
                     /locus_tag="ACFB64_000183"
     CDS             complement(14620..17619)
                     /locus_tag="ACFB64_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /protein_id="HFC1208639.1"
                     /translation="MQNKKPFYEQVAEKLIEQLRQGTAPWQKPWDGGGLPDFMPHNAI
                     TGRRYRGINNLWLQAQGRKDPRWITYKQASSIGAQVRRGEKGTVVEYWQFTEEVPKTD
                     EKGQEIIGQDGKPEKETVRLERPKVFHAVVFNGEQIDGLPPLKLRPLSWNPDERAENI
                     LKNSGAVIDHRHGDRAFYSPSRDSITLPLREQFESPGAYYETALHELGHWTGHADRLN
                     RDLSHPFGSEGYAREELRAEIASLMLSQELGVSFNPGQHASYVASWIMVLQDDPMEIM
                     RAASDAEKIQGYVMAFDQVREIEQQNEAAVSISDRQNIDEASLAEEKNLINAVDKNDV
                     AAIINFMAEEKMHEGLKRFSLVISEMDDATFQNAFWQRHQKPENGESFMENLNAAKEA
                     LDEAIEQSSYRTVEEQIRQFLSEQDKESVRVVSDERVYVQIPYADREQAKMLGAKWDK
                     EEKSWYIPPGVNQEPFRKWLDSATNQAKTVTDNTPAIAKTLIDVPFREKNEARALGAK
                     WDKDQKSWYIPEGVNTALFARWMQPKQEALAEEAVPDDTPQEEAVECQVAAIQQEYQA
                     AKTEEAPAEENASRVYLAVPYEEKEEAKALGAKWDKANKSWFMESSNANLPAASKWLP
                     EKQQTQSMPAMSPVEEFADKLREMGCVVANGHPFMDGQKHRIETVGDKKGERSGFYVG
                     HLDGHPAGYIKNNRTGAEVKWKAKGYSLSPEEKAKLAAEAAQKLQMRSEQKKQAQEAA
                     VGRIKYYMESYLPVQTGTPYLNAKGIKPQPGIFTDREGQKTFIPVYNAAGELRSMQYI
                     QADGTKRFAKDSEQEGCMHVVGQQDLAKAKTIILSEGYATAASIKEATDDTVASVAAF
                     NSGNLPLVAKVLSAKYPQAQFLVAGDDDLAVEAKQGNNPGKEKALEAAKILNCRAVFP
                     VFAPGEQSSEHKAFTDFNDLAQKSKFGREGLAKQINEAIHARPANELQTLKTRGLQEE
                     ASQDRPVEQTKRQQRATTRKTASRGSR"
     gene            complement(17622..17786)
                     /locus_tag="ACFB64_000184"
     CDS             complement(17622..17786)
                     /locus_tag="ACFB64_000184"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208640.1"
                     /translation="MIAEKVADLNNPGMEVDFDPAEAEALGAFEETAMDAETARDSVA
                     DLSREVAEGA"
     gene            complement(17808..20003)
                     /locus_tag="ACFB64_000185"
     CDS             complement(17808..20003)
                     /locus_tag="ACFB64_000185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFC1208641.1"
                     /translation="MSRLYIAEKPEVARAIAAALGVGIERRNGYFRCSNGDNITWCYG
                     HMLALCDPEDYDSKYGNWKFEDLPIAHIPWRKKPLSDKKEQIGVIRDLLAECTEIVHA
                     GDPDDEGQLLVDELLEFLNCAKPVKRVLINDNNERSVKRALSAIEDNAKFRGLSQSAE
                     ARSVADQLYGYNMTRAYTLKAAEQGYQGVLSVGRVQTPILGLVVRRDRENQSHVKTHY
                     YHVYADLDVDGVRFTARYVPNKDQDPIDDKGRLSDPLFARQIAEAASGQPVKILAAKT
                     SAKRQSPPLPYNLLKLQADCSRKFGLKPDQVKDIGQSLKDKHRLITYHRSDCQYLNDE
                     HFDDAADVLDAVGKTASVFKNAIASADTKIKSRAFNPDKVSAHHAIIPTVKTANMDDL
                     TEDEKRVYLLIARAYIAQFWPDHEYNQTDIEAEIAGKKFVCRSNVTVKPGWMALYKND
                     QGNAETATDPEDLSVDLQMLKPGNSGRCLTAEETQFETKPRPLYTMDTLLNDLTRVAK
                     YVQNPKLRELLIEKDKGKEGEHGGIGTPATRDEILRNLLDKGFLLEKGKNIVSSEIGQ
                     QLYDALPDQAKFPDMTALWHEQQQHIVQGGLDAQRFVNSLMEYISGEVERVGREGISL
                     NVKKYPCPECGKPLRRMRGSKGFFWGCTGYKDGCKVSFSDKAGRPVLAPKKAEPSSKF
                     KCMACTHPLVRRPGKQKGTHFWGCSNYPECKQYYPDAGGKPDYSKHTKG"
     gene            complement(20006..20542)
                     /gene="traF"
                     /locus_tag="ACFB64_000186"
     CDS             complement(20006..20542)
                     /gene="traF"
                     /locus_tag="ACFB64_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer signal peptidase TraF"
                     /protein_id="HFC1208642.1"
                     /translation="MRRLFDRAMLAMCLTAALLVTGYGLLYAAGFRINDTRSVAKGVY
                     RISGEAVRRGSYITFCPPDTEVFRMARRRGYIRAGFCPENYSGLMKFVAGIPGDNYRF
                     TDEGLLINGRLLGGTRPLWQDGLGQPLPVFEGEGRLKDDEYILIGNAVANSFDARYYG
                     IVRREHIRDVVKPIWIEE"
     gene            complement(20539..22428)
                     /locus_tag="ACFB64_000187"
     CDS             complement(20539..22428)
                     /locus_tag="ACFB64_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFC1208643.1"
                     /translation="MFRSKNKRNNAVGHQNQAQATGSGRLVNFICLVLVLLAGMGAAT
                     QYFAHQFQYHSQLGYGIGKFYMPWSILIWYGKWHGRYEQQFMTAFSIMLMVVAVGMIG
                     LLVWNIVRKNTNKAHESLHGSARWADRKDIEAAGLLAKKGEDSDGVYVGGWVDKKGTL
                     HYLRHSGPEHVLTYAPTRSGKGLGLVLPTLLSWPHSTFITDLKGELWALTAGWRKKYA
                     HNKVIRFEPASAQGSAAWNPLDEIRVGTEYEIGDVQNLATMIVDPDGKGLNSHWDKTA
                     FALLTGVILHALYKARDDGGVATLPSVDRMLSDPEKPVGDLWVEMATYPHADGRPLPV
                     ISSAGQDMLDRPEEEAGSVLSTAKSFLALYRDNVVAANVSRSDFKIKDLMNDEDPVSL
                     YIITQPNDKARLRPLVRILINMIIRLSADKMDFENGRPKVHYKHKMLAMLDEFPSLGK
                     LEIMQESLAFIAGYGIKCYLICQDLNQLKSRETGYGPDETITSSCHVQNAYPPNRIET
                     AEHLSKLTGITTVVKEQITTSGKRVSAMLGQVSRTTQEVQRPLLTADECMRMPGPVKD
                     ARGLITQAGDMVIYVAGYPAIYGRQTLYFKDPVFEARSKVPPPCHTDRTQKKTANTPS
                     AFDTL"
     gene            complement(22445..24838)
                     /locus_tag="ACFB64_000188"
     CDS             complement(22445..24838)
                     /locus_tag="ACFB64_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFC1208644.1"
                     /translation="MIAKKIPMRKANLSSFEGLVTYLAGSQGVANRVGKVEIHNYESS
                     DLRWAVQETLAVQDQNKRSAADKTYHLMVSLAGGEFLTDEQLSRIEQSMIEALGYAEH
                     QRISVVHRDTDNLHFHVAINKVHPEKKTIHEPYNDFKILGRECRKFEEEYGLQRVQRG
                     GRGNKKIDMEEVAGIESLIGWIRRECGEQIKEAKTWEQLYHVLSDHGLEIKLRGNGFV
                     ISDGEHHVKASSVDREFSKSALEKRLGAFDQKTGVKAKPSKTYREKPLSKANVDDLFR
                     QYKEEKELQKSRRQDFFLHLAQNRDMEIGRIKEKYAFRRSLIRHSSSGIARRILYRML
                     FRQMKDEIRSVRTDYARRRAVFFEQNKGFAWADWLKHRAAEGDVRALDALRRRGKDAG
                     YKGNRFVGKESGGFAGIKVDTVTKKGTVIYAEDGMTIRDTGSVLKLAAIPDDKAVSRA
                     LEIAIGRYGKVLTIGGTRHFQDKVARIAAAKGLDVSFTDGRLNHLYQQYVKEYEYVRE
                     RTDRYRQADGTGRADGRAGQQRGGAGYAGGKPRRYRWHGYDASRNRGFHAGISGYPIP
                     KLSDSGGIGAGRGQSASAGSDSLRGMPERSLVRIQGRTQVLLPRDVSSHLEQQGSANY
                     QAVRRTIHRTGGVAEIRQMQAVVGLVAKPGKKPPGFRRGSLRGMEGLAEIKISSAAVV
                     EIPKNPEPAQENERLTHSLAPIDKYIAERQEKIDKKFDILNHRKYNPSDSGVMLFRGI
                     RNIDGQALALFQPKEVEEILVMSVTPYVSRRLSSVKLGSEVKVTPTGQIRPVKGRHR"
     gene            complement(24841..25218)
                     /locus_tag="ACFB64_000189"
     CDS             complement(24841..25218)
                     /locus_tag="ACFB64_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HFC1208645.1"
                     /translation="MAGARKRPKYISVYVSEEEWKTIESLAKSVGLSQSAYLKLLGLG
                     YKPASVYDGEAVKALAKINADQGRLGGLIKMWLANDERLMMFDHDQLAKAIFGVLEQI
                     GEMQESMLEIVRSRRILAAKKIG"
     gene            25268..25969
                     /locus_tag="ACFB64_000190"
     CDS             25268..25969
                     /locus_tag="ACFB64_000190"
                     /inference="COORDINATES: protein motif:HMM:NF036299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK family protein"
                     /protein_id="HFC1208646.1"
                     /translation="MASLAEQDNPLSLPANKDCDVHVHHISCPNSGVQYAKDYTPNNS
                     IYRTYFDDKPPKNALSQTKNDLLYRIIVFKTILLGSFGKALSKNWQSPIEMIQMGKIE
                     KLVLAELAAMQKKGSSAGNKARFLAMQEDIKILLVDRNIPVISVWRILSEKKYVPYSY
                     ETFNRYCKMYIFKSDTTAADQTDTAQSTQEKPDSIRTDKEPPSPASEPIIAGGNTKSS
                     SGFTFNNNPDGKDLF"
     gene            25970..26695
                     /locus_tag="ACFB64_000191"
     CDS             25970..26695
                     /locus_tag="ACFB64_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HFC1208647.1"
                     /translation="MAIIHISLQAKGGVGKSFLALMLSMFYRHIERPVKIFDTDPANA
                     TLFGFPALEAEKFEIMKKDDIDKRLFDRLFSQILSATDDVIIDVGANAFIPLCSYIIS
                     NNLITMLTQEGHQVIIHTSIVGGQNLKHTVNGFDALCSQFPESTGFVIWLNPFWGEIE
                     FEGKSFEKLKVYEKHKSRIRSIIKIPEWDASLHGRDLAAMLEKQKTFDEALASEDFSI
                     FERQRLNIIKNEMFQTIETAQIV"
     gene            26703..27185
                     /locus_tag="ACFB64_000192"
     CDS             26703..27185
                     /locus_tag="ACFB64_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208648.1"
                     /translation="MDEKVKAILATIVEKNGIALSPKDPIMVVFTALEYMLSEQHAEQ
                     KALTVLFKAQVEEATGNWIQSVHQQTEELVSQQLNTTKEQAKEMSKLLLHETDRLLEQ
                     RITDAVKDFEGKLYGINDNAMNAVELRIKETRNTAILNLTAALIVLLAVIILAFKFTF
                     "
     gene            complement(27242..27778)
                     /locus_tag="ACFB64_000193"
     CDS             complement(27242..27778)
                     /locus_tag="ACFB64_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncP plasmid survival protein KfrC family
                     protein"
                     /protein_id="HFC1208649.1"
                     /translation="MNEDKGLVAKAVAAETEQEELFESRQVIEETFAGVLNTYVLEKT
                     EQVERIEEKLEQLISGQMAKLQALQNARPGMLSMPSKKQTWSANIAVAQSRLNRLHDR
                     LEDVREIRDGMALGVPKIQELAARKLRMEEPDLAKKFDDVQTAVRVHNLHEKQRKEKE
                     QQKRQAGLSLSLGQKIVR"
     gene            complement(27815..28171)
                     /locus_tag="ACFB64_000194"
     CDS             complement(27815..28171)
                     /locus_tag="ACFB64_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KfrB domain-containing protein"
                     /protein_id="HFC1208650.1"
                     /translation="MEAEKQRVLVLNKQHLFETEKDGKWITQAVKPAPANMKPGIYLL
                     HTAKSPQDSVRYEGQIVFQNDSYVYQKTRQSIVKYPCSLFSSVPSIGEVVSISVKDKA
                     ATVEKAGIGEKKTLTR"
     gene            complement(28378..29295)
                     /locus_tag="ACFB64_000195"
     CDS             complement(28378..29295)
                     /locus_tag="ACFB64_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC1208651.1"
                     /translation="MNNSNLGLENIGNLSELLGNAEVSSGTPLRLPLASIKEDPDQPR
                     KEFDEGKIQELANSIQERGVKTPISVRQSSEEGVYVINHGARRYRASLLAGLSEIPAY
                     IDQDYSLIDQAVENIQRDNLTPREIADVIGRLLAEGMKKGQIAEKMGKSPAFVTQHAN
                     LLDLPEPLANAFNSGKVSDLTVINDLNALYKKEPEAVEDLLSNEDEITRSSVRNLKEF
                     LNSDPKNSADADHEKPSKAKVKEKKPEDPDKLKKAIVQVMYQGQLARLVLNKRGLSGD
                     EVWIKFEESGETESVPCKEISGIVAVIEG"
     gene            complement(29301..30056)
                     /locus_tag="ACFB64_000196"
     CDS             complement(29301..30056)
                     /locus_tag="ACFB64_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC1208652.1"
                     /translation="MKILVVANQKGGVGKTAMSLHLAWHMETVGLRVLVIDLDTQGNA
                     SYSLRDKTCLFGSGRLFGNMEDADLHMPGNVSLALSPATNDLANVQNMTLQNAVQSFT
                     QNIDKLKAGGQFDVCIIDTPPSLGNTLAAALAAGDYVLCPIELETYSLQGIKQMAATI
                     GNIRKVNSKLAFLGILPSKVDMRNPRHKRHLEEIKMQYNEIVIPHIIGLRSSIADALS
                     SSLPVWKIKKTAARKAAQEMKAVSNYVLNKMQG"
     gene            complement(30066..30311)
                     /locus_tag="ACFB64_000197"
     CDS             complement(30066..30311)
                     /locus_tag="ACFB64_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208653.1"
                     /translation="MDRIKSKAGKKMFEVVYMVLAIAYLLFPVRWFAWMYSFYVLCQM
                     LYHWNMADSWIYIGKFILTFGIVMGWLTFVIAFRNKI"
     gene            complement(30392..30739)
                     /locus_tag="ACFB64_000198"
     CDS             complement(30392..30739)
                     /locus_tag="ACFB64_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208654.1"
                     /translation="MTDLISTTVLLTPYQAFQAKKWHLQNIEEHNLIQKKDLNIRPEC
                     FHRFPQWAVPTADEVKELWLATGMTQKQVSRYLGLKDTNGRAFRRFISGESSITYAYW
                     VLLSLYVGIEKFW"
     gene            complement(31357..32235)
                     /gene="trfA"
                     /locus_tag="ACFB64_000199"
     CDS             complement(31357..32235)
                     /gene="trfA"
                     /locus_tag="ACFB64_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator TrfA"
                     /protein_id="HFC1208655.1"
                     /translation="MKNVQEVLPYLNLVAERRDEAQREEYLKSKGIPTDDNLIIEDLF
                     GNTIFGSKHRAMPNDFARSCLFTARNHRVPRKTFSREKLFHISEKVEILYTGTELRSV
                     DDELIWMQLVDYCRRFPLGNYVEFDIRQLIKDIGWDTSGAYYKRVRESLSRMKATEIY
                     IKNGNTYGVSGGISLIDNYVGIENIKGEPTRYKVSIDKKLIVLFAGNTFSNIPWSQYK
                     KLSPMARRLTDYVFSHKHPNPIPIPAFLAMCGSDQINSPAKTQNQNAKKICAELTEAQ
                     LVKTAFVLSGKIIVER"
     gene            complement(32239..32622)
                     /locus_tag="ACFB64_000200"
     CDS             complement(32239..32622)
                     /locus_tag="ACFB64_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC1208656.1"
                     /translation="MNKFIISGRTTKDLTLEQTATGVFTTSIPLAENYFYTKDGKKVE
                     ATNYFWIDVYGEQAKNAVRYCGKGSLILIEGRISNNNYEKDGQKIYKDQMIASRIEYS
                     NLKPPAGREESSDPSFIYDPEQDLG"
     gene            32682..33047
                     /locus_tag="ACFB64_000201"
     CDS             32682..33047
                     /locus_tag="ACFB64_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC1208657.1"
                     /translation="MSYSKIFFTNVLRLASEKNLSHQDLAERANLSPSSMSGITRGQG
                     NPTLETMSAIAYALDVPLSYLLEHHDLDPESLSLLDGSQSTLPPNYEKITVILPRYKA
                     FQVKKWNDDALKILAKETK"
     gene            33176..34174
                     /gene="trbB"
                     /locus_tag="ACFB64_000202"
     CDS             33176..34174
                     /gene="trbB"
                     /locus_tag="ACFB64_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HFC1208658.1"
                     /translation="MMETTETADSRKERALNKLKRDLGNEFLEKFMNPNLVEVMFNPK
                     QTGNGVSVYWEEQGKEMVYDERLSDWFKTSRVEAVIKTVAGYHGKVITEDLPILESQF
                     PIDGSRFTAILPPVTASPSFTIRKKPARVFTLDEYVSAGIMTESQRQTIRNAVSEHGN
                     ILVIGGTGSGKTTLINAILYDMSEQNPNERLYISEDTGEIQCHSQNKVQVYTTGSIDL
                     TKLVKTALRYRPDRIIVGEVRGAEALDLLDIWNTGHPGGIATLHANNAALGLERLESL
                     ITRNPAHPAAIRPLVAQVIHYIVSIGKDEKTGRKIREIIKIKGLDEKSGNYVFDYL"
     gene            34276..34659
                     /gene="trbC"
                     /locus_tag="ACFB64_000203"
     CDS             34276..34659
                     /gene="trbC"
                     /locus_tag="ACFB64_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer system pilin TrbC"
                     /protein_id="HFC1208659.1"
                     /translation="MTLSQSQQKWLFFIGLAVFLLLMSSPALASQGQGGGLPYESWLN
                     NLRQSVTGPVAFALSIIGIVVAGGVLIFGGELNAFFRTLIFLVLVMALLVGAQNMMSS
                     FFGGAEVTAAVQAAQHYLLPLASKG"
     gene            34664..34981
                     /gene="trbD"
                     /locus_tag="ACFB64_000204"
     CDS             34664..34981
                     /gene="trbD"
                     /locus_tag="ACFB64_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010676131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFC1208660.1"
                     /translation="MRAIRTIPIRRVANRPNLFWGGDREMVQVSLLITFVLVVAAQNT
                     YAFVIGLVFWFFALYALRKLAKYDPLLRFVGLRSITKYKRFYPPHSTPFRNNTSTQGN
                     RYK"
     gene            34978..37542
                     /locus_tag="ACFB64_000205"
     CDS             34978..37542
                     /locus_tag="ACFB64_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HFC1208661.1"
                     /translation="MIIGIISAISVLGLVLCTGLFLKIRAVTNATKLKQHRSKEEGLA
                     DLLNYAAVIDDGVILGKNGSLMAAWIYRGKDMGSSTVAEREDVSLRINQAFVGLGSGW
                     MVHVDAARRPAPNYTEAGRNYFDSYVTEAIDNERRQAFEAIGTLYEGFFVIVATYFPP
                     LLAEKKVAELMFDDDNAKEGKRAQQDRTLSHFKQACANLESNLSISLELHRLCAHKVV
                     LEDGMEITQDDFLRWLHFCITGKNHPINLPAAPMYIDSLIGGQELYTGVVPMIGDKFI
                     QVVALDGFPMESYPGILTRLGEMPIEYRWSNRFIFMDQHQAVADLEKYRKKWKQKVRG
                     FFDQVFNTHSGSIDQDAQAMVDDAEAALAETKSGLVAQGYYTSVVILMHEDRAFLESM
                     ARQVEKEINTLGFNARVETVNTMEAWLGSLPGHGIENVRRPLLNTLNFADMIPTSSIW
                     TGLEENPCQFYPPNSPPLMQVVTSGSSPFRLGLHVGDVGHTLVFGPTGAGKSTLLATL
                     AAQADRYRNMRVFSFDKGRSLETLTRAVGGSHYNIGDEDNTASGLSFAPMQLIQNTSD
                     RAWALSWVEAVMELNGLKVTPQQRNEIETAIKNNAAEQSYSLSDLSNTLQDVEMREVL
                     KAYTIDGSMGYLLDAEQDSLSFSRFTCFEIEELMNLPEKYALPIMLYLFRRIEKALTG
                     DPAVLFLDEAWIMLGHPVFREKIREWLKVLRKKNCLVLLATQSLSDAANSGILDVLNE
                     SCPTKIYLPNAHAREEDASELYRRMGLNARQIEILATAIPKRQYYYTSPYGRRLFELA
                     LDPLTLAFVAVSDPDTLNAMRQTADQYGEDWPIRWLQNRGINIHNYVDVDKYRRKH"
     gene            37542..38249
                     /locus_tag="ACFB64_000206"
     CDS             37542..38249
                     /locus_tag="ACFB64_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VirB8/TrbF family protein"
                     /protein_id="HFC1208662.1"
                     /translation="MGLKDIFRVREKADREMTENPYLNARKQWNFIMGDMVSSRLLWQ
                     WAGMTGLLIGLGGVGGMIYLGSQSKFIPYIVEVNKLGETVAVARADQPQATDERIVRA
                     MLGSFIGNARTVTTDIALQRKYVLNTYAMLNPKDAAAAKMNEWLNGTPESSPFKRAEK
                     ELVSVEIKSVLRQSDNSWQVDWVETTRAHDGKLLKPPQNMRALLNIYYVPPTTEQQII
                     VNPIGMFIRDFSWSNEL"
     gene            38262..39128
                     /gene="trbG"
                     /locus_tag="ACFB64_000207"
     CDS             38262..39128
                     /gene="trbG"
                     /locus_tag="ACFB64_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HFC1208663.1"
                     /translation="MKRQILSLALITALSTSAFADSYADKYFEGSHNPKLNRHEQKGL
                     GIAKNYSSDGRMAVPPVPGDNGRITFAFGSDPSVVCAVMQVCDIALQPGEMINDLHAG
                     DTARWNITPAISGSNGNERMHILVKPLDVGLQTTLFIATDRRTYHIRLKSHRTRYMPA
                     VGFSYPEDTAARWRAIRANQARQAEAQVLPTGENIQNLDFNYTISGHNPSWKPLRVYN
                     DGTRTIIQMPPTLSQGEAPALLVVRNGENTLVNYRLHEDRHIVDSLFDEAIMITGVGR
                     HQTKVVIRRSRS"
     gene            39125..39535
                     /locus_tag="ACFB64_000208"
     CDS             39125..39535
                     /locus_tag="ACFB64_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbH"
                     /protein_id="HFC1208664.1"
                     /translation="MKSNVLFVALALAVGGCATGQYGSFVRSDAVSASVTQDVVEKLQ
                     EEFPAANTVFFLNRSKSDTFGTQLDQALREKGFATTAQQGENEKSLNYVLDQVDSDIY
                     RVMIYIDGTPYSRAYRQTENGAVVPTGSWTKRGD"
     gene            39539..40978
                     /locus_tag="ACFB64_000209"
     CDS             39539..40978
                     /locus_tag="ACFB64_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFC1208665.1"
                     /translation="MSQSTEDLLNASRSPSIAPDTSGRRPGVRRLNNIPIYIVGGVVL
                     GFALLIALVAATKGQQSAGQATQGDGKDATRMADSLLNELPQEGGLITAKASEPAMPA
                     LPDGMASAPDLPASAPTSLPMVPDMANLPATPPEVDQEAEQIRQAKAQMFQAALAAPT
                     KVDVVASTSRGSSGLYTAALSSSGSGTPNRSEMLAQIAEVQRMSENAGSNPAEVERVY
                     NQQLANAQQMAAAGGQDGVQGAGGMPGLNTPPTPGNIPRQTAQNGGNNWRLESQVERP
                     NAYMIRSGAVIPATLISGINSDLPGMVQAQISQNVYDTATGRHLLLPQGSRLVGTYSS
                     GINLGQRRLFIAWNRIIFPDGKALDIGDMPGASGAGYAGFSDRINNHYGRLWGNALML
                     SLVGASITYGVDKNSNNNNNGNNNGNNSTVRGALSESLGQTFGQAVSQSIQKNMNVSP
                     TLEIRPGYRFNVILTKDIDFSKPYRSFDY"
     gene            40991..41767
                     /gene="trbJ"
                     /locus_tag="ACFB64_000210"
     CDS             40991..41767
                     /gene="trbJ"
                     /locus_tag="ACFB64_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFC1208666.1"
                     /translation="MQKRLLSKTKTVALSLAVSVSLGLSAPAYAGIPVTDGGNIAQSS
                     VSAIQNVAQTLKQIEEYRIQLKQYETQLRNSLAPAAYVWDQANKTMANMKAAVARLRA
                     YKQQLGNMEEYLSKFQAVDFYKSSPCFTAKGCSAADRAKLEQNYAQLSRMQKLSNDGL
                     LRSINNEIDSISRDANTLTQLQRNAQSATGQLEALQYANQFHSAQAHQLLQLRNMINT
                     EMQANAAYRQSQLDREARYEAARKQVRTSDILKHPYRRGQ"
BASE COUNT        10541 a        10914 c         9223 g        11326 t
ORIGIN      
        1 aaaaaaaaat atatttatta ttttatattt tttataacgc tctctatgcc aattatctct
       61 catgcagcag gtcaaggagg agttttagat gaggcattat cacgctacga aggtaaaatc
      121 ggagcgtggg aagcagcatt ttataaagct ggattattta tattttggtc attatcttca
      181 attagtattg taatgactgg agcccaactg attttccaga gagataatat cagcagtttt
      241 tttgctgaat ttactcgttt aattttattc ttaggatttt tcctttggat tatcacaaac
      301 ggaattaaaa ttacgacatc aattaaagat ggtttaatga aacttgcagg acaagctagt
      361 agaaatggtg agcagattac acctagtagt attgttgaca taggttttaa tttatttgac
      421 aaaattgttg aaaatagctc aatctgggat ccaattgata gtgccactct tattgttatt
      481 ggtttagtta ttttactaat cttagctgct gtcggaatta acatgttatt aatgttaata
      541 acaacctata ttgtcatcta ttcaggaata attatcttgg gattcggcgg tggacgttgg
      601 acatcagata tggctgtcgg atatttaaaa caagtattaa acttatcttt acaattagca
      661 gctatgattc taattattgg aataggtcaa tcgattgttc aagacactat aaacactata
      721 ggaacccctg gatttagaga actagcagtt gttctagttc aaacagcttt acttgttggt
      781 ttagtcgtta aaattcctcc aatgataggt tccctagcag gaggagcagg aagtggagga
      841 attggctcct ttggtgtagg taatgctatt gctgctgctg ctatgttggc aggtgctgct
      901 gccggagcag gtgcggcatt aaaaagctta gccgtagaag ctggtggttt aaagaaagca
      961 tttgaagctg caaaagcagg cttgggaggt tcaaatggct catcagattc aggtagtggg
     1021 aagatggata gcgcagttga tgccgttgca ggtgcaaatg ccggagataa atctaaccaa
     1081 gatggaaaac cactgactgc tgagtccagt ggtaactcag aaaatggtag tgaaggatct
     1141 actacaggaa attcgggagg taatccatca ggctcctccg aagaaaatac aatttcatcc
     1201 ggaagctcct caagttcggg ggaagcaaat tcaacaaata catccgcttc atcaggagcg
     1261 gaaggtagtg ccgaagggga ggctacttca tcagagacat ctcaatcagc ttctgattcg
     1321 ccaagcaata atagcggaga ggcgactcaa acggctgaca ccacaccaaa aacaactaat
     1381 accggtggag gaagtcctct tgctactgcg atgggtggta ataaagtatc aaattggcag
     1441 gcggcaaaaa acgttgctgt cggccttgtt agcagcaaat ggaacaatgc cgtcaataac
     1501 tctgccggtg gccgcctagt caaacactgg gaggacgaaa agaaaaaaac agaagacccc
     1561 acaaaaaatc cccaaaaaaa ccctgaagga gatgacaatg cttaaaaaaa cgcttatctg
     1621 catattgctg gtcggttccg tacactttgc tatggccgaa agcctcaacg atgcgctgaa
     1681 ccgcagccgt tcgggaaact cgaacaacaa caggccgcag gttgaagact tgttcaccgg
     1741 tgatgtaaag ctggcctgcg aagccacgtt atgcctgtcc acgggcaacc ggccgagtga
     1801 atgccaaccg gccatccacc gttatttctc tatccgccac aaacggatgg gagacacaat
     1861 tcgcgcccga cacaacttcc tgcgtttatg cccggcatct aaagagcaag gcatgctcga
     1921 actggttaat gccattgcca acggtgccgg tcgatgtgat gcccgggaac tgaaccgtat
     1981 caaccgttcc acttatatca agcgtgtttg ggtcggatca agacggcatg gagactggca
     2041 agaggtcaaa gtcccctata tccgtaacgc caaaccttcg tactgctctg cctactttga
     2101 aaacggctgg acacagcttg gcacacgcta tgtaggtgaa gagaaaaatg gcgggaaatg
     2161 ggttgactaa tggtttatgt ggccgacctg cccccattta tgcaggaacg tgttacctgc
     2221 tcgattgctg cggctgaaaa ataccatctg ccgccacatt acctgttggc cgtggccgaa
     2281 cgcgaaaacg gtcgtcccgg tcaacaggta cgcaatacca acggcaccta tgatgtaggc
     2341 gcgatgcaga ttaataccgc ctatatccgc accctatccc gttacggtgt tactcagtca
     2401 gccgttgccc aacccggatg ctatgcctat tacttggccg catggcggat caagcggcac
     2461 atcgtttatg atagaaaggg agatatttgg acaaagatcg caaactacca ttcctatacg
     2521 cctaaataca acgcaatcta tcgcgcagcc atcattcggt tggccgaaaa atgggaacgg
     2581 tggcttgccg gtaggcaagg gaataacgcc aagggaaatg ttcccacaca agctgttcca
     2641 gccgttcgga caattcggtc gcaaccattg cccaagcaat atgtttcccc gacgaccgtc
     2701 tatgttccta gaacaatccg tgtggtagag cgttccccgg agccagagcc ggaaaccgtc
     2761 caaaactaac agtaaagacc atctgaaaaa gagtttcaga cggtctttta taatttcccc
     2821 ttgacatgga tggtaaattc atgtttatat caattcaatt atttaggagg tatggaaatg
     2881 gcttacgcaa ttgcatttga tttggatacg accgttttac aagaacttta ccctaatagt
     2941 agctggcaaa atgcttacgg tgatgtaaaa agaaccttgg tcggattagg ttttaaccat
     3001 cagcaaggca gtgtatattt tggaaactct gagattacag ccgttagctg tgtcttggcc
     3061 gcccaaaaac tgagccagac ctatgcatgg tttaagccgg ccgtttcgga tattcgtatg
     3121 ttgcggattg aggaaatgaa cgatttgcag cccgctttat aaagcgatag gtatcttaga
     3181 aaagggcagc gcggtgtccc cgccctgccc tttccctttc cttccagcgt tgccgagtaa
     3241 aggctgccta gtttctacta ggcagcccaa actactttgc cttgcaagcc cgtttaaccg
     3301 tactttcgct aacattgaaa tgtgcggccg tccgggaaat actggtttgt tcagactgcc
     3361 gccatcggcg gatttcttcg ggatcgactt tcactttccg ccccagtttt ttcccttctt
     3421 ccctagcatt gcggataccg gccatttgac gctctttgat gtaactgcgt tccaactccg
     3481 caaatgcgct gacaatagcc agcatcgccg tacctaacgg actggccaaa tcgaagtttt
     3541 cccggagcga ggttacggcc acgccctttt tctgcaaaaa ctgcaccgtc tccaaaacat
     3601 cgatggtatt tctgcccaaa cggtcaatgg ctactacgac tacggtatcc ccttggcgta
     3661 caaattcagc catcttacgg aactcaggcc ggttaagggc ttttactgca cccgaagcgg
     3721 cctgatcttc ataccaccgt tcgatctggt atttgtcttg aataacctgt ctttgtgcgg
     3781 ccacattctg ctctttggtc gataccctca aatagccgat aatcataatt cctcaaacgg
     3841 attaaaaact cttgcgccca agtgtgaaaa atccttttca ttgcgcgtta ccaccatcat
     3901 atgctcctgt ccaacggtcg tcttcgataa gcacacggtc aaactcaatg tctgcaatat
     3961 ctacctcttc cggaggaacg gccaaggcat cagcgatact ctccccctta cccaacgatt
     4021 ctgctcctaa cgaaaggtca tggggacagc cattcaaaaa cggagacaac cgtaccttat
     4081 aagccggaaa atcggtaaca aactttctca gtttttcatc aaccatcctt tgaacaaatg
     4141 gattgcctgt cgctgcctcg atgttgtgca aaatgtcggc caaatctccg attatcgtca
     4201 aattatccag cgcagccgca taactctcag gccgataccc gcgccgggaa taagacagtt
     4261 ccctgataat cagcgaaatt tccgtgatca accgcatttt ttctttcatt tccgtatccc
     4321 tgcttatctg ctgatggatt ttttggcgga atactccaaa gacttgacaa tcaatttgcc
     4381 cttattgctt ccgatagaaa cgttctgccc tatctcaacc aatttggcca gtctttcttc
     4441 gttatgtgtt gcattgtgcc tgacaagccc gtttttggtt ttttgaaata ccgcagcggc
     4501 atccttgtaa atgatttctc cggaataagt ttgaccagtc tctgcttttt tagcttgacc
     4561 tagtaaatag ataccggctt tcatgcctgt tacaggaatt tgttccacct tagaccacaa
     4621 ctcgtttgct gactttgttt ctagggtaac acccttggag ttagtaacta ccctttgctg
     4681 ggaatactct tttttggcga aaacactttc aagatccata ctgcccttgg cctgattgaa
     4741 gcattcccgg ctgatttttc ctgagtcgaa gtcgctctgg attttctcgg ccaacctgcc
     4801 caatatttgc tcttgcgacc ccaaggaaag cattttgatg acatcatcga acttgtcgat
     4861 aaattcactg ttcctgtctt ggttgatacc gacaattttt accgcatcac caaacttatc
     4921 cctgatttgc ttgagtccgt caggaagatt ggcctgaata tcagccataa tacctatact
     4981 ggctcccctg ccgtattcgt taaaccgttt gtaagtattg tcggaagccc tttcagccct
     5041 catgctgacg gctacgatag taacttcaag attacgctcc agacactgct caatcttgcg
     5101 aaaagcagac tgcggccgtg caagctgtcc ttcaaaaagc agcttggtgg tatcctgcat
     5161 catcatgttt tttactgtcg aagtttttcc cgaacccgga attcccgtaa cgaaaataac
     5221 ttctttcttc tgtggctcgt cgcgtttcag tacctcgtca aactgggtag aagataaaac
     5281 tgctgctgaa ttgtggatgg cattattaac cgttgccctg tcttctttgt tctcaaaagc
     5341 tggaaacaac tctttaaacg tatcagcaca aacatatcta ccgccttctg tatctgcatg
     5401 ttggctatat aaagacaata tctcgtctgt ttgttcggtc gcaaaacgga tggcctcatc
     5461 ctgaacggaa gacaaatatt cgttattgcc aaaatctctc aaattaatat cgctagacag
     5521 ttttaccata gcggattatt gctccttatt tgccgcccac aacactctca acttagctag
     5581 aagctccgga gatgggccgg catacaagcg tttttccact tctttgcgga aggcttcgtc
     5641 tgtcatcatc ctgcggtttt tttcgcaagc ctcttttatc attctgatag cattactttc
     5701 ttggggctca actttattca taataaaatc ctaatcttaa ttttatatta ctcatcatcg
     5761 cttcatcgtt ttctttaatg aggcatctgc cggtttaacg gacaattcat ttccttcccg
     5821 cttaatagaa accttctgac caatttccac ctgttcggac aaactagaaa actttccttc
     5881 cgccataccc tgaaccgcct tatggcgtat cagaccttgg tcagtcttct ggaaaaccga
     5941 tgcctcgtct ttatggacaa tctctccgct gtattccctg ccggatttag ccggtttcgc
     6001 cgtgccaagc atgtgaatac cggctttcat ccccttggct tcagccttct caatatcctg
     6061 ccatgtgtcg cctgatttgc gttgcagtgt tgccccttct gaattagcca ccaccctttc
     6121 ctgacggtaa atctcttcca cacgctgata aattgcttca ctatgctgtt tgaccatgcc
     6181 gagataatta ggtattggat cgatactgcg gctttctgcc tgaagctgta tctcgtccca
     6241 tgcttttaca acctgtttca accgtttagg ggttatttcc tgataagccg atagtgcatc
     6301 ttttgccgtt tcatgctggt tttgtcgccg attcaaactg tcataaagga tgtctcctgc
     6361 gcggttggta acacgaaccc tgtcaaaact tgattcacat tgagacaacg tatccttgaa
     6421 tccagccatt gcctcgttat ggtacgactc ttcaacaaat ctaggctgtg aaacaccttt
     6481 ggcatgttgt tcctcataac gcttaaaaat cccggctaca ctctcttcag aagcagtggc
     6541 taccgccagc atttctaccc cgtacccttc tgattttcta tcacggataa attggctgat
     6601 acttgcagca tttctaaatg tcccctcctc cacaatattc cgccggtttt cttttgcact
     6661 attccgaaca ctaatggcca aagcaccggc atctttctga gtttgttcag acgaatacac
     6721 caccccctcc ggcgcaggaa tcagtgcacg cataatatct gcatccacat ggatatatcc
     6781 gccttcttgg cgtaactcgc ttttagctaa agaagctgcc ttactcttac ccgctccggg
     6841 ttgtgcccca actagaacaa ttttgggttg ctccacactt tgcgttctag ataaacgtgg
     6901 cttcatgaaa ccattcagaa ttcttgcctt accttcggca cttaaagggg tgtactcaat
     6961 cattttacac gtccaactgt ttggtcatcc ggattgaaaa tacgcgcgat aatatctgtg
     7021 tcctccggag agagactgtc acgcaacata tcgatagcct caagccttgc tgtacagtat
     7081 tcgataaacg cttggtttgg tttatccttt acaaactcgg actctcttgc ctgaattaat
     7141 gggcgcatcg aatctcccaa caactccaaa gccacttcat aattgatacc aggaatagtt
     7201 tcttgcattt gcatcttggc ttcctccaaa agttatccac aaattttgtt agtaagctgc
     7261 ccatatcgcc agcctgccac atataataaa ttgaatcttc gtcatcaaac cgtttttgcc
     7321 tgctttttaa gcaacatggt tcaaaaccta tgtttgaata ataagccagt tcaaaataaa
     7381 taacaacgtt tttgacctat acttccagac cttaacgaaa catggttcat aaagtacact
     7441 ttttgcacct acttatctaa gttacccacc tcttaatcaa gatttggcca ttgcccacca
     7501 tatcccataa tgtgtagaaa ccagtagcgt tgggtggatg acggcgtttc gggttttccg
     7561 caggcggcag gcagcctgca atggtagtgg ctaatcctgc gcgctattgc atgagacaca
     7621 aacttcaaga tagcgcagca aggctttttc tatcgtttgt cgattaacat caaggtagaa
     7681 gagagactta tattcggatg caatgccttt tgctaggttg gcaataaaca tcttgtcgtc
     7741 ttcatctata tcaatatggc tgataaaagt cggtttggcc aaattcttga cagtaagata
     7801 ggcattgaat tctccaacat tcatatccga gaaatagtat tgcagctcta tcggtttcat
     7861 tttttcttcg attttggctt caatattctt caccgattcc cgtcgttgtg cttctttcag
     7921 gcagcctgaa aagttgtttt tcagtgccaa tgcgtgcagc agagcgtaca gggcatcgtt
     7981 accggctgcc gacagcggag gcacggattc gagcagtccg ccggagacgg tttcggctgc
     8041 cgcaccgtag cggccgttct ctttcgtcag gcggataatg atttcgtatt cttggacggt
     8101 tttcatgttc atactcccag ttgtctttgt tcagtccgtg ctaacggcgt atccagccgc
     8161 actccggcgg cggcatcctg cccgactctc tttcacgttt ataattatag gataatttat
     8221 cctacttgtc aaataaattt tagccgctaa atttttactc tcgcttattt attcattata
     8281 ataaattaat cgttattttg tttaactgta aaattggaga ttccttgaca aatatgctct
     8341 tacgtgctat tatttaagtg actatttaaa aggagttaat aaatatgcgg caaggtattc
     8401 ttaaataaac tgtcaatttg atagcgggaa caaataatta gatgtccttt tttaggaggg
     8461 cttagttttt tgtacccagt ttaagaatac ctttatcatg tgattctaaa gtatccagag
     8521 aatatctgta tgctttgtat acctatggtt atgcataaaa atcccagtga taaaagtatt
     8581 tatcactggg atttttatgc ccttttgggt ttttgaatgg aggaaaatca catgaaaatt
     8641 attaatattg gagttttagc tcatgttgat gcaggaaaaa ctaccttaac agaaagctta
     8701 ttatataaca gtggagcgat tacagaatta ggaagcgtgg acaaaggtac aacgaggacg
     8761 gataatacgc ttttagaacg tcagagagga attacaattc agacaggaat aacctctttt
     8821 cagtgggaaa atacgaaggt gaacatcata gacacgccag gacatatgga tttcttagca
     8881 gaagtatatc gttcattatc agttttagat ggggcaattc tactgatttc tgcaaaagat
     8941 ggcgtacaag cacaaactcg tatattattt catgcactta ggaaaatggg gattcccaca
     9001 atctttttta tcaataagat tgaccaaaat ggaattgatt tatcaacggt ttatcaggat
     9061 attaaagaga aactttctgc cgaaattgta atcaaacaga aggtagaact gtatcctaat
     9121 atgtgtgtga cgaactttac cgaatctgaa caatgggata tggtaataga aggaaatgat
     9181 taccttttgg agaaatatac gtctgggaaa ttattggaag cattagaact cgaacaagag
     9241 gaaagcataa gatttcataa ttgttccctg ttccctgttt atcacggaag tgcaaaaaac
     9301 aatataggga ttgataacct tatagaagtg attacgaata aattttattc atcaacacat
     9361 cgaggtcagt ctgaactttg cggaaaagtt ttcaaaattg agtattcgga aaaaagacag
     9421 cgtcttgcat atatacgtct ttatagtggc gtactgcatt tgcgagatcc ggttagaata
     9481 tcggaaaagg aaaaaataaa aattacagaa atgtatactt caataaatgg tgaattatgt
     9541 aaaatcgata aggcttattc cggggaaatt gttattttgc agaatgagtt tttgaagtta
     9601 aatagtgttc ttggagatac aaagctattg ccacagagag agagaattga aaatcccctc
     9661 cctctgctgc aaacgactgt tgaaccgagc aaacctcaac aaagggaaat gttacttgat
     9721 gcacttttag aaatctccga cagtgacccg cttctgcgat attatgtgga ttctgcgaca
     9781 catgaaatca tactttcttt cttagggaaa gtacaaatgg aagtgacttg tgctctgctg
     9841 caagaaaagt atcatgtgga gatagaaata aaagagccta cagtcattta tatggaaaga
     9901 ccgttaaaaa aagcagagta taccattcac atcgaagttc caccgaatcc tttctgggct
     9961 tccattggtc tatctgtagc accgcttcca ttagggagcg gagtacagta tgagagctcg
    10021 gtttctcttg gatacttaaa tcaatcgttt caaaatgcag ttatggaggg gatacgctat
    10081 ggctgtgaac aaggattgta tggttggaat gtgacggact gtaaaatctg ttttaagtat
    10141 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
    10201 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
    10261 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
    10321 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
    10381 cggtgtattc aaaaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
    10441 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
    10501 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
    10561 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaaaatta
    10621 actgtgagtg attggagaca ccatgccaca tgagcaaatg attgatgctt tttcagcagt
    10681 cattcaaaac tccttaagcc tatatgaaga gcaaactccg atatactact acctgactat
    10741 ctgcggttct tgggttctga ctgcactcgg catcctctcc ttggtgtccg tattctttac
    10801 catcaagcag aaaaaaccat accgttttat cgtcccgatt ctgtttttat ggctgggata
    10861 tgaaagcatc cacatgccgc gcccgaatct tgctgtattc tacggataca acttggcgca
    10921 gctttcctat ccggcaaacg aaaatcccaa ctcgttccta catctcgcag acggctcaaa
    10981 tccgatttgc tgcttcatag gcgaagacga gtatttgcaa gccaaacggt taggtttcaa
    11041 agagtatgcc gatgcaaaag caaaggcttt ggttgatttg gttgaaatgg agaaaaaatc
    11101 caaacaatct gattgaatct taaattcaat aataacgcgg tagggggctg gtcaagcgaa
    11161 ggttcaggct ctgactgaaa tttcgcttgg acagggggtg tggggcggag cgcagcgaag
    11221 ggctaccgcg tggcggccga agccgcccga caaactccag tttgtcgcta ttccgttttc
    11281 agtgcgtaaa tacctttccc cactctcaga aaaaaccgct cgtcctgtaa tacctgcctg
    11341 actttggcct gccagttcct gttttctgcc cgcgcagcgg catacggttc catttcgcca
    11401 taaatttccg tcaagcccat attcctcccg tctgccgcct gcaatacccg tcttacggcg
    11461 gctttccatg taacggccgt taccgcctca accctattca tcgctttagc caaaaagtac
    11521 aaggccgtct gaatctcttt tttaaagacc agcagtttct catgggcaat ccgtaccagc
    11581 tttccgccat attggcggct gtcgctgacg cagttgtgct ggattttgat aatctcatcc
    11641 accagccgcc ccgccgccat ccgttcaacc atgctgctca aattgtagta acggccgtcc
    11701 ttcctcatgt tgcccattag aatcccataa tgcccgccct gttcggtcgc atcatggatg
    11761 ttcatcatgg ccagcagcat agcctcttga aactgttcca agtccatttg gctcaaatcc
    11821 cacggattgg ctgcatcacc ccattggttg ccactgtatc gaatcatgtt ccaataaggc
    11881 ggatgccacc aaattaaatt ggcctgttct cctaaaaatg ccgcaaaatc ctgagtcagc
    11941 aggttatatc cctgatgcaa atccgttcct ttaaaacgaa tgcccatttc ctccgcaaca
    12001 tccgaactgg taccgctgcc gatgctcggg tcggcaaaca gtccttccgg agcaagatag
    12061 ctttccacaa agtcgcggat gatatgcccc gtgcaatttc cgcgataacc gcttttaccg
    12121 taatttccgc gctgcggata agaacaaaca gaatgcgtaa acatggttca ttcctccttt
    12181 aaatcgtctt cattttcgcg ataatggcaa cctaatactg catccaaatc ccgcatggtt
    12241 tccaccggct cgtcattgac ataaaagtgt gtatccagcg cattgattaa aaaacccgtc
    12301 cgcaagaacg gccaccggtt gcggatggta tcctgcaaac gctccagcaa atgatgctgc
    12361 cgaaggtttc caaaatgcgg aacggtttca tatgtccgtg ccgtttccca aatgtcctgc
    12421 tcgaagttga taaacgagtc gccgccgagt tggcgcagca gattcatcca accatcgaaa
    12481 ctgtcggaca catcatccca ataaagcata atttcctcct tatcactcaa tccctgcatc
    12541 ggccaaaatc aaagccaaga tgccgctgcg ccgtgctttg gcagttttca gtttcagaca
    12601 ctgcaaatca tcgcaaacaa tctgataccc gcgcgtctcc agttcggccg cttctgacac
    12661 ttgcaggctg taatactgcc cgccatcgaa tgtgtatcgg caaagcagtg taggggcatt
    12721 tcgtccgtcc cacataacgt taatcggact gaatcccatc aactccccat tgctccaaat
    12781 attctgctcg acaaatacat tcggatgccc agttaatctg ttggtatagc ttgccatctc
    12841 tgtctcccgt actttccttg gtgttaaacg gtttttgaac tgaaccagca tcggcgtttc
    12901 cgccccgctg tgatgacgtt aaaagacaaa ctgctttgag atacaaggct gccgcaggca
    12961 ccgcaggctc ggccttgtat ctcaaagcag tttgtcttag gaatcacgca acagcggggc
    13021 gggaatgccg gttgcgtcga acggccaaaa gccgtttgac accaaggaaa accttctgcc
    13081 ggccgcagcc ggctcattgc ccctcacggg gaaggggtgg ggttcggaga ggcggtgccg
    13141 cagcacggcc gatacttaaa ccaagagcgg tttgatgcct tagcatgaaa ccgctctttc
    13201 cgttcaatcc tgctgactct tgattagcct gaaactccca gcaccttcaa gataaccgtt
    13261 tatagtcttg cgaatggtaa catattccgt ccccatcatt ccgaatccgc ccttagtcca
    13321 aaaaaacggc tccctcggca aaaaaggaat atattcctcc tcttgaggct cgcccatgcc
    13381 aagactgatc tcgggctgat tcccatccgt ctcataatgc agcttttcgt cccctttgaa
    13441 aaaatcggcg acgaccgctt ccgcctcttc aagagattgg ctgataaata aagccgaact
    13501 accaaagccg atctcacctg tctctacaag gtagaaagtt cgattttcag cggacagact
    13561 cattttctcc actcccgata aacgcctatg tcgttatgga taaaggttgg atagataccg
    13621 atcccaccta ccctgaacct tcttgccaaa gcggcaagct cggaaaggga cgctctttcg
    13681 atatgaaagt cccccgcatt gccggacaaa tgttgggagt ttttggccgc cccctctaac
    13741 gagtcgttat gtcggcgcgt gcggaatccg ctatgcaaaa ccaactccgg attcggtctg
    13801 cccaaatcac gcaaatactg ctgctgggca aacagcagat tgatcaaatt gatgttgatg
    13861 gcgtgcatct gatggttgtc caccacatcc cgcatcatcc aacacagagc cttatagccc
    13921 tcttggatat actggccgtc ggcaaagaat ttgatgtttt tccgttcccg ggaagcaggc
    13981 cggtaaatgg acaatacccg gtttcgtttc caaaaccggc tgtcatccgc caaaacgatt
    14041 tccggcaaca gtaaaaggcc gcctgatgaa gcaagcgcgg ccgctttggc cgacagcttt
    14101 aaaaaatccc gacgattcaa cgctccctcc ctttcacgtt accgctgacc ataaaagcat
    14161 accggccgta ttcgttacct ccctcattcg ccgtttctcc ggccgcaagg gatcccaaaa
    14221 cctgctctaa tcccatttcg accgcaggta taaaatcttc atctgtcgcc gtaatggtaa
    14281 tgctgagcgt cttggtatcg gcatgcgtat ctacccgatg attcagtccg tactgcttct
    14341 gaaatgcctt gacggcggcc tgttcggact gattccgttc ggccgacacg cccatttttt
    14401 cctcttcgtg ccgctgcaac atttccaaat aatcgtcaaa cgccaagggc tggttctggc
    14461 tggttttccg gtaatcgacg gcgatatttt gcagcgtctg ccgcaggcgg ccgtcaaagg
    14521 ccatgccgaa aacccgccag aaccatcctt ccctggcctg tttgtgataa gtcagaaaaa
    14581 aagccactct gtataatttg tccttatcca taacaccccc tatcggctgc cgcgcgatgc
    14641 ggttttccgg gttgttgccc gctgctgccg cttggtctgt tcgacaggcc gatcctgaga
    14701 cgcttcttcc tgcaaaccgc gtgttttcag ggtttgcaat tcgtttgccg gtcgggcatg
    14761 tatcgcttcg ttgatttgct tggccaaacc ttcgcggccg aacttggatt tctgagccaa
    14821 atcgttgaaa tcggtaaatg ccttatgctc cgacgactgc tctcccggtg caaacactgg
    14881 aaacactgca cggcagttaa ggatttttgc cgcctccaac gctttttctt tgccgggatt
    14941 gttgccctgc ttggcttcga cagccaaatc atcgtctccg gcgaccaaaa actgagcctg
    15001 agggtatttc gcagacaaga ctttggccac caaaggcagg ttgccggaat tgaacgcggc
    15061 cacactggcc accgtatcgt cggttgcttc tttaatcgag gcggccgtag catagccttc
    15121 cgataaaata atggttttgg ccttagccag atcctgttgg ccgacaacgt gcatacaacc
    15181 ctcctgctcg ctgtctttgg caaaacgttt ggtaccgtcc gcctgaatgt attgcatgga
    15241 gcgtaactcc ccggctgcgt tgtacacggg aatgaaagtt ttctgcccct cgcggtcggt
    15301 aaagataccc ggctgcggct tgataccctt ggcattcaga taaggcgtgc ctgtttgaac
    15361 gggcaggtag ctttccatat agtatttgat acggccgaca gcagcttcct gagcctgttt
    15421 tttctgctcg ctccgcattt gcagcttctg agcggcctcg gcggccagct tggctttttc
    15481 ctccggagac aggctgtatc cctttgcctt ccacttaacc tccgctcccg tccggttgtt
    15541 tttgatgtag cctgccgggt ggccgtccaa atgaccaaca taaaagccgc tgcgctcgcc
    15601 ttttttgtcg ccgacggttt cgattcggtg tttttgtccg tccataaacg gatgcccatt
    15661 agccaccaca caccccattt cgcgcaactt gtcggcaaac tcttccaccg gcgacatcgc
    15721 gggcatgctt tgagtctgtt gcttttccgg caaccatttt gacgcggccg gtaaattagc
    15781 gttactgctt tccataaacc aagatttgtt ggctttgtcc catttggcac caagggcttt
    15841 agcctcttct ttttcttcat acggaacggc aagataaaca cgggatgcgt tttcttctgc
    15901 cggtgcttct tctgttttgg cagcctgata ctcttgctgt atggctgcga cctgacactc
    15961 aactgcttct tcctgaggag tatcgtccgg cacggcctct tctgccaacg cttcctgctt
    16021 cggctgcatc caccgtgcaa acagtgcggt attcacgcct tcggggatat accaagactt
    16081 ctgatcctta tcccatttcg cgcccaatgc ccgcgcttcg tttttttcac gaaacggcac
    16141 atcaattagc gttttcgcta tggccggagt attgtccgtt accgtttttg cttggttggt
    16201 ggcactgtcc agccattttc tgaaaggctc ttgattgacc ccgggaggga tgtaccacga
    16261 tttctcctcc ttatcccatt tcgcgcccag catttttgcc tgttccctgt cggcataagg
    16321 aatttgaacg taaacgcgct catcggaaac cacgcggacg ctttccttgt cctgctccga
    16381 cagaaactgg cggatctgtt cttcaaccgt acgataagag gactgctcga tggcttcgtc
    16441 caaagcctct ttggccgcat tcagattttc cataaatgac tctccattct ccggtttttg
    16501 atgccgctgc caaaaagcgt tctgaaacgt ggcatcatcc atctccgaaa taaccagact
    16561 gaaacgcttc aagccctcgt gcattttctc ttcagccatg aagttgatga tcgccgccac
    16621 atcattttta tcaaccgcat tgataaggtt cttttcttcc gccaagctgg cttcatcaat
    16681 attctgtcta tcagaaatac tcactgccgc ctcgttctgc tgttcgattt cccgcacctg
    16741 atcaaaggcc atgacatagc cttggatttt ttcggcgtca gaagccgcac gcatgatttc
    16801 catcggatcg tcctgcaata ccataatcca gcttgctaca taactggcat gttgcccggg
    16861 attgaatgaa acgcccaatt cctgactcag catcaatgaa gcgatttcgg cacgcaattc
    16921 ctcccgtgcg taaccctcgc tgccgaacgg gtgggaaagg tcgcggttca aacggtcagc
    16981 atgccccgtc caatgcccca attcatgcag ggctgtttca taatacgctc ccggactttc
    17041 aaactgctcg cgcagcggca gggtaatact gtccctgctc ggagagtaaa acgccctgtc
    17101 gccgtgccgg tggtcgatga ccgcaccaga atttttcaga atgttttctg cccgctcatc
    17161 cggattccaa cttagcggcc gcagcttcaa cggcggcaag ccgtctatct gttcgccgtt
    17221 aaataccacc gcatgaaaga cctttggacg ctccaaacga accgtttcct tctccggttt
    17281 gccgtcctgg ccgataattt cctgaccctt ttcatcggtt ttgggaactt cctcggtgaa
    17341 ctgccaatat tcgacaacag tacccttctc tccacggcgc acctgcgccc cgatggatga
    17401 tgcctgttta taggttatcc agcgcggatc ctttctgcct tgtgcctgca accacagatt
    17461 gttaatgccg cgataacggc ggccggtaat ggcattgtgc ggcataaaat ccggcaaccc
    17521 gccgccgtcc caaggtttct gccacggcgc ggttccctgc cgcaactgtt cgatcagttt
    17581 ttccgccacc tgttcgtaaa acggtttttt attctgcatc cctacgctcc ttccgccact
    17641 tcgcgggaaa ggtctgccac ggaatcccgc gcggtttccg catccattgc ggtctcctca
    17701 aacgccccca aggcttctgc ctctgccgga tcaaaatcca cttccattcc cgggttattc
    17761 aaatccgcca ctttttctgc gatcacttcg atgtcgatac ttatacctta tcctttcgta
    17821 tgtttggaat agtccggctt accgccagca tcggggtaat actgcttgca ctcggggtaa
    17881 ttgctgcatc cccaaaaatg cgttcctttc tgttttcccg gccgtctgac caagggatgg
    17941 gtgcaggcca tgcacttgaa ttttgacgac ggctcggctt ttttcggtgc cagtaccggc
    18001 cgaccggcct tgtcggaaaa agaaaccttg catccgtcct tatagccggt acagccccaa
    18061 aaaaatcctt tcgatccgcg catccgcctt aaaggtttgc cacattcggg acacgggtac
    18121 ttcttgacat tgagactgat gccttcccgc cctacccgct ccacctcgcc ggatatgtac
    18181 tccatcagac tgttcacaaa acgttgcgcg tccaaaccgc cttggacaat atgctgttgc
    18241 tgctcatgcc ataaggctgt catatcgggg aacttcgcct gatcgggcag cgcgtcataa
    18301 agctgttggc cgatttcgga actgacaatg tttttgcctt tttccagcag aaaacccttg
    18361 tccagcagat tacgcagaat ttcgtcccga gtggccgggg tgccgattcc gccgtgctct
    18421 ccttccttac ccttgtcttt ctcaatcagc agttcgcgca atttcggatt ctgcacatac
    18481 ttggctaccc gtgttaaatc gttcaacagc gtatccatcg tgtataacgg gcgcggtttg
    18541 gtttcaaact gcgtctcttc tgctgtcaaa caccggccgc tgtttcccgg tttgagcatc
    18601 tgcaaatcaa cactcaaatc ctcgggatct gtcgcggttt ccgcattgcc ctgatcgttc
    18661 ttgtatagag ccatccaacc gggctttacg gttacgttgc tgcggcagac aaatttttta
    18721 ccggcaattt ctgcttcaat gtcggtttgg ttatattcgt gatccggcca aaactgggcg
    18781 atataggcgc gggcaatcag cagatagacc cgtttttcgt cttcggtcag gtcgtccata
    18841 ttggccgttt tgacggtagg aataatggca tggtgcgccg aaactttatc cgggttgaac
    18901 gccctgcttt tgattttcgt gtcggccgat gcaatggcat ttttaaagac tgatgcggtt
    18961 tttccgacag catccagcac gtctgccgca tcatcgaaat gctcgtcatt gagatactgg
    19021 cagtcgctgc ggtggtaggt aatcaaacgg tgtttgtctt tcaatgactg tccgatgtct
    19081 tttacctgat cgggtttcaa accgaatttg cgcgaacaat ccgcctgtag tttgagcaga
    19141 ttgtatggca gcggcggact ttgccgcttg gcagaagttt tagccgctaa aatttttacc
    19201 ggctgaccac tggctgcttc ggctatctgc cgtgcaaaca gcggatcgga caaacgtccc
    19261 ttatcgtcta tcggatcttg atccttgttt ggaacgtaac gggcagtaaa ccggacacca
    19321 tccacatcca aatcggcata cacatggtaa taatgggttt tgacatggga ctggttttcc
    19381 cgatcgcgcc gcaccactaa acccaaaatc ggagtctgta ccctgccgac ggacaacact
    19441 ccttgataac cctgttccgc agccttcagc gtataggctc gggtcatgtt gtatccgtat
    19501 aactggtctg ccaccgatcg cgcttccgcc gattgggaca atcccctgaa tttggcgttg
    19561 tcttctattg ccgataacgc tctctttaca gatcgctcgt tattgtcgtt aatcagtacc
    19621 cgtttgaccg gtttggcaca attcaaaaac tccaacagtt cgtcaaccaa caactgtcct
    19681 tcgtcatcgg gatctccggc gtgaacaatc tcggtacatt ccgccagcaa atcacgaata
    19741 accccgatct gttctttttt gtcggacaag ggttttttac gccaagggat atgcgcgatc
    19801 ggtaaatcct caaacttcca gttaccgtat ttgctgtcgt aatcttccgg atcgcacaaa
    19861 gcaagcatat ggccgtaaca ccaagtgatg ttgtctccgt tactgcaacg gaaatacccg
    19921 ttgcgcctct cgataccaac tcccaaagcg gcggcaatgg ctctggccac ttcgggtttt
    19981 tctgcaatat aaagtcggga catacctact cctctatcca tatcggtttt acgacatcac
    20041 ggatatgctc tctccggact atgccgtaat aacgcgcgtc aaacgaattg gccaccgcat
    20101 taccaatcaa aatgtactca tcgtctttca gacggccttc cccttcaaat accggtaagg
    20161 gctgccctag cccgtcttgc cataaaggcc gggtgccgcc aagcagccgt ccgttaatca
    20221 acaacccttc atcggtaaaa cggtaattgt cgccgggaat accggcaaca aacttcatca
    20281 acccggaata attttccgga caaaaacccg ctctgatata ccccctcctt cttgccattc
    20341 ggaaaacttc ggtatcggga gggcaaaatg taatgtagct tccgcgccgt accgcttcgc
    20401 cggatatgcg gtaaacccct ttggccacac tgcgggtatc gttgatacgg aaaccggctg
    20461 catataacag cccgtagcct gttaccagca atgcggcggt cagacacatg gcaagcatgg
    20521 cacggtcaaa cagccttctc atagagtgtc aaacgcgctg ggcgtattgg cagtcttttt
    20581 ctgagtccgg tcggtatggc acggaggcgg gactttggag cgcgcctcaa ataccggatc
    20641 tttgaaatac agggtctgcc gcccgtaaat cgccggatac ccggcaacat aaatgaccat
    20701 atcgcctgcc tgagtgatca aaccccttgc gtccttgacc ggcccgggca tacgcataca
    20761 ttcgtcggct gtcagcaacg gccgctggac ttcctgcgta gtccgggaaa cctgccccaa
    20821 catcgcactt acccgtttgc cgctggttgt gatctgctct ttgacaaccg ttgtgatgcc
    20881 ggtcagcttg gacaggtgtt ccgccgtctc gatccggtta ggcggataag cgttctgcac
    20941 atggcagctg gacgtaatgg tttcatccgg gccatacccc gtttcccggc ttttcagttg
    21001 gttcaagtct tggcagatca aataacattt gatgccgtaa ccggcgataa acgccaagga
    21061 ttcctgcatg atttccaact tgcccaaact cgggaactcg tccagcatgg ccagcatctt
    21121 gtgtttgtag tggactttcg gcctcccgtt ttcaaaatcc atcttgtcgg ccgacagacg
    21181 gataatcata ttgatcagaa tccgaaccaa aggccgcaac cgcgccttat cgttgggctg
    21241 ggtaatgatg tacagactga ccggatcttc gtcgttcatc agatccttga ttttgaaatc
    21301 ggaacgactg acattggcgg cgaccacatt gtcgcggtac aaagcgagga aagatttggc
    21361 tgtggaaaga accgatccgg cctcctcttc cggtctgtcc agcatatcct gtccggcaga
    21421 agaaatgacc ggcagaggac ggccgtctgc atggggatac gtcgccattt ccacccataa
    21481 atcgcctacc ggtttctccg gatcagacag catgcggtca acgcttggta aggtggcaac
    21541 cccgccatca tccctagctt tgtaaagcgc atgcagaatg acgccggtca gcaaagcgaa
    21601 agctgttttg tcccagtggc tgttcagccc cttgccgtcc ggatcgacaa tcattgtggc
    21661 cagattctga acatcgccta tctcatactc cgtccccacc ctgatctcat ccaaaggatt
    21721 ccatgccgcg ctgccttgcg cggaagccgg ttcaaagcgg attaccttgt tgtgcgcgta
    21781 tttcttccgc cacccggccg tcaatgccca taattcgccc ttcaaatcgg taatgaacgt
    21841 actgtgcggc catgacagca aagtcggcaa taccaaaccc aaacccttac cggatcgggt
    21901 tggggcataa gtcaggacat gctccggccc ggaatggcgc agataatgca gggtaccctt
    21961 tttatcgacc cagccaccga catatacacc gtcgctgtcc tctccttttt tggccagcag
    22021 accggccgcc tcgatgtctt tcctgtccgc ccaacgcgcc gacccgtgca gagactcatg
    22081 cgccttattg gtattcttcc gcacaatatt ccataccaac agccctatca tgccgacggc
    22141 taccaccatc aacatgatgc tgaatgccgt cataaactgc tgctcgtacc gtccgtgcca
    22201 tttaccgtac caaatcagaa tgctccacgg catatagaac ttgccgatgc cgtatcccaa
    22261 ctgggaatgg tattggaact gatgggcaaa atattgtgtg gctgccccca taccagccaa
    22321 cagcaccaat accagacaaa taaagtttac caaacggccg cttcctgtcg cttgggcttg
    22381 gttctgatgg ccgaccgcat tgttgcgttt gttcttgctg cgaaacatgt ttatcctttg
    22441 aaatttaacg atgccttccc ttaacaggtc tgatctgccc cgtcggagta actttcactt
    22501 cactgcccag tttcacgctc gaaaggcgtc tggacacata gggtgtaacg gacatgacca
    22561 gtatttcctc gacctctttc ggctggaata aagccagtgc ctgcccgtct atgttgcgga
    22621 tgcccctgaa caacatcaca ccggaatcac ttggattata cttcctatga ttcaatatat
    22681 cgaatttttt gtcaattttc tcctgccgct cggcaatata tttgtcgata ggtgccaatg
    22741 aatgagtaag tctctcgttt tcttgtgcag gctcaggatt tttcggaatc tctactaccg
    22801 cagcggatga aattttgatt tcagccaacc cttccatccc ccgcaaactg ccccgcctga
    22861 atcccggcgg tttttttccc ggtttggcaa ccaaaccgac aactgcctgc atctgcctga
    22921 tctcagctac tcctcctgtc cgatgtattg tccgtcgcac agcctgatag tttgcggatc
    22981 cttgctgctc caagtgacta gaaacatcac gcggcaatag cacttgagtt cgtccttgga
    23041 tgcgaaccag actgcgttcg ggcattcctc gcagactgtc gcttcctgcg gaagcagact
    23101 gccctcttcc tgctccaatg cctccagagt cggagagctt cgggattggg tatcctgata
    23161 tgcctgcatg gaagcctcga tttcggcttg cgtcataccc atgccatcga tagcgtctag
    23221 gctttcctcc tgcatatccc gcgcctcctc gttgctgccc tgcccgccca tctgcgcggc
    23281 cagttccatc agcttgtctg tatcgatcgg tacgttccct gacatattcg tattccttca
    23341 catactgttg ataaagatgg ttcagacggc cgtctgtaaa cgacacatcc aaaccctttg
    23401 cggcggcaat tctggccacc ttatcctgaa aatgccgcgt tccgccgata gtcaggactt
    23461 ttccgtatcg ccctattgcg atttccaacg cccttgatac cgccttatcg tcaggaatcg
    23521 cggccagttt gagcacactg cccgtatcac ggatggtcat gccgtcctcg gcataaataa
    23581 ccgtcccctt tttcgttacc gtatcaacct tgatcccggc aaatccgcca ctttctttgc
    23641 cgacaaagcg gttgcccttg taacctgcat ccttccccct ccttctcaac gcatctaacg
    23701 cccggacatc gccttcggca gcacggtgtt tcagccaatc tgcccaagca aaacccttgt
    23761 tctgctcaaa aaaaaccgca cgtcggcggg cataatcggt tcttaccgat ctgatttcat
    23821 ccttcatttg cctaaacagc atccgataca gaatgcgcct tgcaatacca ctgctgctat
    23881 gcctaatcag gctgcggcga aaagcgtatt tttctttgat gcggccgatc tccatatccc
    23941 ggttttgtgc caaatgcagg aaaaaatcct gccgcctgct tttctgaagc tctttttcct
    24001 ccttgtattg ccggaacaga tcgtccacgt tggctttgct caacggtttc tcacggtacg
    24061 ttttggatgg tttcgccttt actcctgttt tctgatcaaa tgcaccaagc cttttttcca
    24121 acgccgactt ggaaaactcc ctatctaccg aacttgcctt gacatgatgc tcgccgtcgc
    24181 tgataacaaa accgttaccc ctcagcttga tttccaatcc gtgatcactc agtacatggt
    24241 acagttgctc ccatgtctta gcctctttaa tctgctcacc gcactctctc ctgatccatc
    24301 ctatcaggct ctcgataccc gccacttcct ccatatcgat ttttttattg ccgcgcccgc
    24361 cacgctgcac cctctgcaaa ccgtactctt cttcaaactt cctgcactcc cgccctaaaa
    24421 ttttgaagtc gttatacggc tcgtggatgg tctttttttc tgggtgcact ttgttaatcg
    24481 ctacatgaaa atgcagattg tcggtatctc tgtgaacaac agagatccgc tgatgttcgg
    24541 cataccccaa agcctcaatc atgctctgct caatacggct caactgttcg tccgtcaaaa
    24601 attccccgcc cgcaagggat accatcagat gataggtttt atccgccgca ctgcgcttat
    24661 tctgatcctg aactgccaaa gtctcctgaa ccgcccacct caaatccgat gattcatagt
    24721 tatgaatctc cacctttccc actcggtttg cgacaccttg agaaccggcc agataggtaa
    24781 ctaagccttc aaaactgctc aggttggctt tacgcatcgg aatttttttt gcaatcatgt
    24841 tcagcctatt tttttagccg ctaaaattct gcggctccgc acaatctcca acatactttc
    24901 ctgcatttct ccaatttgct ccaacactcc gaagatcgcc ttagccaact gatcgtgatc
    24961 gaacatcatc agccgctcgt cgttggccaa ccacatcttg atcaagccgc ccaatcggcc
    25021 ttggtcggca ttgattttgg ccaaggcttt aaccgcttca ccgtcataaa cggatgcagg
    25081 cttgtatccc aagccaagta atttcaaata agccgattgg ctcaatccga cagatttagc
    25141 caaactctct atggttttcc attcttcttc cgaaacgtaa acgctgatgt attttggccg
    25201 ttttcgtgcg cctgccattc ttggagtcct taatttgtgc cgcaaggcac acaacagcca
    25261 acggtagttg gccagcctcg cagagcaaga taatccgttg agcctaccgg cgaataagga
    25321 ttgtgatgta catgtacatc acatatcttg ccccaattct ggggttcaat acgccaagga
    25381 ttatacgcca aataattcaa tatatcgaac atattttgat gacaaacctc ctaaaaatgc
    25441 tttatcacaa acaaaaaatg atttactata tcgaattatt gtttttaaaa cgattttgct
    25501 tggcagtttt ggcaaggctt tgtcaaaaaa ttggcaaagc cctatcgaaa tgatccaaat
    25561 gggaaaaata gaaaaactgg ttttggccga acttgccgcc atgcagaaaa aaggctcctc
    25621 agccggaaac aaggcaaggt tcttggccat gcaggaagac attaagatac tgctggtgga
    25681 caggaatatc cccgttatca gtgtttggcg gattttgtcg gaaaaaaaat atgtcccata
    25741 ttcctatgag acatttaacc gctactgcaa gatgtatatc ttcaaatccg acactacggc
    25801 cgctgaccaa acagacaccg cccagtccac acaggaaaaa cctgattcca tccgcacgga
    25861 caaagaaccg ccgtctcccg cctccgaacc cattatcgcg ggaggcaaca ccaaaagctc
    25921 ttcaggcttt actttcaaca acaacccaga cggaaaggac ttattttaaa tggccattat
    25981 ccatatcagc ctccaagcaa aagggggcgt cggtaaaagt ttcttggctt tgatgctgtc
    26041 tatgttttac cgccatatcg aaagaccggt aaaaattttc gataccgatc cggccaacgc
    26101 aaccctgttc ggttttcccg cactggaggc ggaaaaattc gagatcatga aaaaggatga
    26161 tattgacaaa cggttgtttg accggctttt ctcacaaatc ctgtctgcaa ccgatgatgt
    26221 catcatcgat gttggcgcca atgcgtttat tccgctttgt tcgtatatca tttccaacaa
    26281 tctgattacc atgctgacgc aggaaggcca tcaggtcata atccacactt ccattgtcgg
    26341 cggccagaac ctgaagcata cggtcaacgg atttgatgcc ctatgctcgc aatttccgga
    26401 atcaaccggt ttcgtcattt ggcttaatcc gttttggggg gaaattgagt ttgaaggtaa
    26461 gtcgtttgag aaattgaaag tctatgagaa acacaaatcc cggatacgtt ccatcattaa
    26521 gattccggaa tgggacgctt ctctgcacgg ccgcgacctt gccgccatgc tggaaaagca
    26581 aaaaacattc gacgaagcct tagcgtccga agatttcagc atctttgaac gccaacgttt
    26641 gaacattatc aaaaacgaaa tgttccaaac gatagaaacc gcccaaatcg tctgagtaca
    26701 tcatggatga aaaagtaaaa gccatattag ccaccattgt tgaaaaaaac ggcatcgcct
    26761 tatctcccaa agacccgata atggttgtat tcactgcctt agaatatatg ctctccgaac
    26821 agcacgcaga gcaaaaggct ctaaccgtct tattcaaagc gcaggtagaa gaggccacgg
    26881 gaaactggat acagtccgtt caccaacaga ctgaggaact tgtcagccag caactcaata
    26941 cgaccaaaga acaggcaaag gaaatgtcca aattactgct tcatgagaca gacaggctgc
    27001 ttgagcagag aattactgat gccgtaaaag actttgaagg aaagctatac ggcatcaacg
    27061 acaacgccat gaatgcagta gaattgagaa taaaggaaac tcggaacacg gcaattctca
    27121 atctgacggc cgccctgatt gtcttgttag cagtcattat cttggcgttc aaatttacct
    27181 tctgacaaaa aacccggcaa tgccgggttt tttgtctcca ttctttacgc caagagaaaa
    27241 atcagcgtac tattttttga cctagggaca ggcttaatcc agcttgccgt ttctgttgtt
    27301 ctttttcttt gcgctgtttt tcatgcagat tgtgaaccct gacagccgtt tgaacatcat
    27361 caaatttttt tgccagatcc ggttcctcca tccttaactt cctagcagcc aactcctgta
    27421 ttttcggaac acccagagcc atcccgtcgc gaatctcgcg aacatcttcc aacctgtcat
    27481 gcagacggtt cagccttgat tgtgccactg caatgtttgc actccacgtc tgttttttgg
    27541 acggcatgct caacattccc ggccgggcgt tttgcaatgc ctgcaacttg gccatttgcc
    27601 cggaaatcaa ttgctccaat ttttcttcta tgcgctcaac ctgttctgtc ttttccaaga
    27661 cataagtatt taacacaccg gcgaaagttt cctcaataac ctgcctgctt tcaaacaact
    27721 cttcttgttc ggtttcagcc gcaacagctt tagctacaag ccctttatct tcattcatgg
    27781 tgttatccta aaattttagc ggctaaaatt tcatttagcg cgttaaagtt tttttctctc
    27841 cgatacccgc cttttctact gttgccgctt tgtccttgac actgattgag accacttctc
    27901 cgatcgacgg aacggaagaa aatagagaac acgggtattt gactatagac tgtcgtgttt
    27961 tctgataaac atagctgtca ttttgaaaaa cgatttgccc ttcgtacctg acagaatcct
    28021 gaggagattt ggccgtatgc agcaaataaa ttcccggctt catattggca ggagcaggct
    28081 taaccgcctg cgtaatccat ttgccgtctt tttctgtctc gaacaaatgc tgtttgttca
    28141 ataccagcac tctttgtttt tctgcttcca ttctgctaaa aattttcaaa aaagatgtga
    28201 ataattctaa cagaacagtc tttaatatac ccccccccca ccataaatat ttgattttaa
    28261 aatgatttaa tttaatgaat tctgatcaac ctgctgattc ggtattttgc acataccggc
    28321 agcagtaaaa ctgggcagcc cgttaaaacg ggggggtatt ataataaatt cccagtatta
    28381 tccttcaata actgcaacaa tcccggaaat ttctttacaa ggaacagatt ctgtttcgcc
    28441 ggactcctca aacttaatcc atacctcatc gccagacaat ccgcgcttgt tcaaaaccag
    28501 ccttgccagt tggccttgat acattacctg cacaatagct tttttcagtt tgtcaggatc
    28561 ttccggcttt ttctctttca cttttgcctt ggatggcttt tcatgatcgg catcagcaga
    28621 atttttagga tccgagttta aaaactcttt caaattcctt accgaactac gggtaatctc
    28681 gtcttcgtta gacagcaaat cttccaccgc ttccggttct tttttataca atgcgttcag
    28741 gtcattgatt accgtcaaat cggatacttt ccccgagttg aatgcgttgg ccagaggttc
    28801 gggtaagtcc agcaggtttg catgctgggt aacgaaagca ggggatttgc ccattttttc
    28861 ggcaatctgt ccctttttca tcccctctgc cagcaatcgg ccaattacgt cggcgatttc
    28921 ccttggcgtt agattatcgc gctggatatt ttcaacagcc tgatcgatca ggctgtaatc
    28981 ttgatcgata tatgcgggaa tctcgctcag ccctgccagc aatgaagccc tgtaacgtcg
    29041 tgcgccgtgg ttgatgacat agaccccttc ttcggaactt tgacgcaccg atatgggtgt
    29101 tttcacgcct ctctcctgaa tgctgtttgc tagttcctga atttttcctt cgtcaaattc
    29161 ttttcttggt tgatcggggt cttctttgat tgaagcaagg ggcaggcgca gcggcgtccc
    29221 tgatgagacc tctgcattac ccaaaagttc gctgagattg ccgatgtttt ccaaacccaa
    29281 attactgtta ttcataaatt ttacccttgc attttgttta aaacatagtt agaaacggct
    29341 ttcatttcct gcgccgcctt tcttgctgcc gttttcttga ttttccaaac cggcaggctg
    29401 gaagacaacg catctgcgat actgctcctc aatccgataa tatgcggtat tacaatttca
    29461 ttatactgca ttttaatttc ttccaaatgc cttttatggc gcggattcct catatcgacc
    29521 ttacttggca atatacccaa aaaagccaat ttgctgttca cttttctgat attgcctatt
    29581 gttgcagcca tctgtttaat tccttgtagg ctgtatgttt ccaactcgat cggacataac
    29641 acataatccc cggccgccag tgcagccgcc aacgtgttac ctaaactggg cggagtatca
    29701 ataatacaga catcgaattg cccgcccgct tttaatttgt cgatgttttg cgtaaaagac
    29761 tgaacagcat tttgcaaagt catattctgc acgttagcca aatcgtttgt cgccggggaa
    29821 agagccagtg aaacgttgcc gggcatatgt aaatccgcat cttccatatt tccaaacaaa
    29881 cggccggaac cgaaaagaca cgttttgtct cgaagagagt aactcgcatt cccctgcgta
    29941 tccaaatcaa ttactaaaac cctcagtcca actgtttcca tatgccaggc aagatgaagc
    30001 gacatagccg tttttcctac cccgcccttt tggtttgcca ccactaaaat cttcattcct
    30061 acacctcata ttttatttct gaacgcgata acaaaagtga gccatcccat tacgataccg
    30121 aacgtcaaga taaatttgcc gatataaatc catgaatcag ccatattcca gtggtacagc
    30181 atttggcaca aaacatagaa actgtacatc caagcaaacc atcggacagg gaaaagcaaa
    30241 taggcgatag ctaacaccat ataaaccact tcaaacattt tttttccagc tttggattta
    30301 attctgtcca taggagcctc cgtccgatat agtttgtatt ataggataaa ttatcctatt
    30361 tgtcaagcaa ttttagcggc taaaatttta tttaccaaaa tttctcaata cccacataca
    30421 agctcaataa aacccaatag gcatatgtga tggatgattc cccactgatg aatctcctga
    30481 aagctctacc attggtgtct ttcaacccta aatacctgct aacttgtttt tgagtcattc
    30541 ccgttgctaa ccacaattct ttgacctcat cagccgtagg gactgcccat tgcggaaacc
    30601 ggtggaaaca ttctggacgg atattcaggt cttttttttg tatcaagtta tgctcttcaa
    30661 tattttgaag atgccatttc tttgcttgaa atgcttggta aggagtaagt aatacggtgg
    30721 tagaaattaa atcggtcatt tgaaagttct cggcaattta cccttggagt aaaatgaaaa
    30781 agatctaaat cgcccgtagg gcgacagacg aatttatgaa attatctaaa atcgccgtag
    30841 gcgacagcaa attgataaaa ccggcttttc ggtcgcgaaa tatggttata aatggttaga
    30901 taatggttag ggcaaaaaaa agcggttttt ttcaattaat attcagtatg ttgcaaacat
    30961 ggcggcatgt gtaaatccgc gatttagcat gtgtaaatcc gcgatttagc atgtgtaaat
    31021 ccgcaaggga attaggatat tttgtcctaa aagcatgtgt aaatccgtaa attttgctca
    31081 tattttaggc gataccgaat gttatgcaca ggttatccac agttttgatt tttaaaatag
    31141 gttttttata tttattttgt ctgtttgttt tttaattttt atcgcatatc ttgacctgca
    31201 tattttggta tttttgtcaa accagccatc ggcatgtgta aatccgcaaa tggcatgtgt
    31261 aaatccgcaa acagtgaaaa tttgctgttt tttggcatgt gtaaatccgc aaatggcctg
    31321 tttggggcat gtgtaaatcc gtgcttgtgg ataattttaa cgctcgacga taatcttccc
    31381 ggataataca aaggcagttt ttaccaactg cgcctcggtg agttcggcac aaatcttttt
    31441 agcattttga ttttgcgttt tcgctgggga gttgatttgg tcgctgccgc acattgctaa
    31501 aaatgcagga ataggtatag ggttgggatg tttgtggcta aagacataat cggtgagccg
    31561 cctagccatt ggagaaagtt ttttgtattg actccaaggg atgttggaaa atgtgtttcc
    31621 ggcaaaaagc acgattagtt ttttatcgat gctgactttg taacgtgtcg gttcgccttt
    31681 gatgttttca attccgacat aattgtcaat aagggaaata cctccgctga cgccgtaggt
    31741 atttccgttt ttgatataga tctctgtcgc tttcattctg ctcaaagatt cccggacacg
    31801 tttataatag gctccgctgg tatcccatcc gatgtcctta ataagctgcc tgatgtcaaa
    31861 ttcgacgtaa ttccccagag ggaaacgacg gcagtaatct acaagctgca tccatatcag
    31921 ctcgtcatcc actgaacgga gctcggtacc tgtgtaaagg atctctactt tttctgagat
    31981 atggaaaagt ttttcacggg agaaagtttt tctcggcacc cgatgatttc ttgctgtaaa
    32041 caaacatgat cgggcgaaat cgttcggcat cgctctatgc tttgaaccaa agatagtatt
    32101 tccaaacaaa tcctctataa taaggttgtc gtctgtcgga atgccttttg atttcaagta
    32161 ttcttccctt tgggcttcat ccctgcgttc ggcgactaaa ttcaaataag gaagcacttc
    32221 ctgaacattt ttcataattt atcctaagtc ttgttcgggg tcgtagataa atgaggggtc
    32281 tgatgattct tcccgtccgg cagggggttt gaggttgctg tattcaatac ggctggcaat
    32341 catctggtct ttatagattt tttgcccatc tttttcatag ttgttattgc ttatccttcc
    32401 ttctatcagg ataagggaac ctttgccgca atacctgacg gcgttcttgg cctgttctcc
    32461 atatacatct atccaaaaat agtttgtggc ctcaactttc ttcccatcct tggtgtaaaa
    32521 atagttttct gccaaaggaa tactggttgt aaaaactcct gttgcagttt gctccaaagt
    32581 caaatctttg gttgttctgc ctgaaatgat gaatttattc attctgcctc ctaggttact
    32641 ttgctatact tacggttaaa aattttagcg gctaaaattc catgagttat tcgaaaatat
    32701 tcttcactaa tgttctgcgt cttgcatccg aaaagaatct gtcccaccaa gatttggcag
    32761 aaagggcgaa tttgtccccg tcttcaatgt cgggaattac aagagggcag ggcaatccga
    32821 cattggaaac catgtcggcc attgcttacg cccttgatgt cccgttgagc tatttgctcg
    32881 aacaccacga tcttgatccg gagagccttt cccttctaga tggaagtcaa tcaacactcc
    32941 cgcctaatta cgagaagatt acggtcattt tgccgcgtta taaggcattc caagttaaaa
    33001 agtggaacga tgatgcctta aagattttgg caaaagaaac caaatagcaa cattcattag
    33061 gttattttat cccatatctg ccagatatgg gctttttttt tggctcttga acaattagac
    33121 tttacatttt attgaattca atatattgta tcaactctat tgtatggttc tcttaatgat
    33181 ggaaacgacg gaaacagcag actcccgcaa agaacgggca ttgaacaaac taaagcgtga
    33241 tttgggaaat gagtttttgg aaaagttcat gaatccaaac ttggttgaag tgatgttcaa
    33301 tccaaaacag accggaaacg gcgtgtctgt ttactgggaa gagcaaggca aggagatggt
    33361 ctatgacgag aggttgagcg attggttcaa aacctcccgc gtggaagcgg ttattaaaac
    33421 cgttgccggt tatcacggca aagtaatcac cgaagacctt cctatcttgg agtcccaatt
    33481 tcccatcgat gggagtcgtt ttacggccat cttgccgccg gtaaccgcat caccctcttt
    33541 taccatcagg aaaaaacctg cccgtgtatt cactttggat gagtatgtct cagcaggaat
    33601 catgacggaa agccagcggc agaccatccg caatgcagtc agtgagcacg gaaacatctt
    33661 ggttatcggc ggaacaggtt caggtaaaac cacgttgatt aacgccatcc tctatgacat
    33721 gtcggagcag aatcccaatg agcgtctcta catctctgag gataccgggg aaatccaatg
    33781 ccattcgcaa aacaaagtgc aggtttacac cacgggaagc atcgatctaa ccaaacttgt
    33841 gaaaaccgcg ctccgctacc gtcccgacag aatcatcgta ggcgaagtaa ggggggcgga
    33901 agccttggat cttttggata tttggaacac gggacacccg ggcggcatcg ccacactgca
    33961 tgcaaacaat gccgccttgg gactggaacg cttggaaagc ctgattaccc gcaatcctgc
    34021 ccatccggca gcgatacggc cgctggtggc gcaagtgatc cactacatcg tatccatcgg
    34081 caaagacgag aaaaccggcc gcaaaatacg tgagatcatc aaaattaagg ggcttgacga
    34141 aaagtcaggc aattatgtgt ttgattacct ttaagaggaa cagggaggca atttaaacag
    34201 cgggattgaa tactgatgtt ttcataagca acgaacgatt ttttaacaac cacgttatat
    34261 ctgtgaggta aatatatgac tctgagtcaa tcgcaacaaa aatggctgtt cttcatcgga
    34321 ctggccgtct tcttactgtt aatgtcctcg cccgcgctcg cgtcgcaagg gcagggtggc
    34381 ggtctgccct atgaaagctg gctgaacaat ctccgccagt ccgttaccgg cccggtggct
    34441 ttcgccctgt ccatcatcgg cattgtcgtt gccggtggcg tactgatctt cggcggcgaa
    34501 ctgaacgcgt ttttccgcac tttgattttc cttgtgttgg taatggcttt gctggtagga
    34561 gcgcaaaaca tgatgtcctc tttcttcggc ggcgcggaag ttaccgctgc cgtgcaggcc
    34621 gcacagcatt accttcttcc cttggcgtcg aaaggataaa accatgagag ccatccgcac
    34681 gatacccatc cgcagagtgg caaaccgtcc caacctcttt tggggtggag atcgcgagat
    34741 ggttcaggta tcccttttga ttacttttgt tttagtggtg gcggcgcaaa acacctatgc
    34801 tttcgtaatc ggcttggtat tttggttttt tgcgctttac gcactccgga aactggctaa
    34861 atacgatcct ttgctgcgtt ttgtcggcct gcggtcgatt accaagtaca aacgttttta
    34921 tccgccccac tccacaccgt ttagaaacaa tacttcaacc caaggaaacc gttacaaatg
    34981 atcattggga ttatctcggc catatcggtt ttgggcttgg ttctgtgtac gggtctgttc
    35041 ctgaaaatcc gcgccgttac caatgccacc aaactcaaac agcaccgctc gaaagaagaa
    35101 gggctggccg atttgctgaa ctatgcggcc gtaatcgatg acggcgtaat cttagggaaa
    35161 aatggctccc tgatggctgc atggatttat cgcggcaaag acatgggcag cagcacggta
    35221 gcggagcgcg aagacgtatc tttacgcatc aatcaggcgt ttgtcggctt gggcagcggc
    35281 tggatggtgc acgtcgatgc cgcacgccgt cccgcaccaa actataccga ggcaggcagg
    35341 aactactttg acagttacgt tacagaggcc atcgataacg aaagacggca ggcatttgag
    35401 gccatcggca ccctgtatga aggtttcttc gtcattgtcg ctacctattt cccgccctta
    35461 ctggcagaaa aaaaggtcgc cgaactgatg tttgatgacg acaacgccaa ggaaggtaaa
    35521 cgcgcccagc aagaccgcac actctctcat ttcaaacagg cttgtgcgaa tctggaatcc
    35581 aacctgagca tttccttgga gttgcaccgt ctttgcgccc ataaagtcgt tttggaagac
    35641 ggcatggaaa ttacccaaga cgatttcctg cgctggctgc atttctgcat taccggcaaa
    35701 aaccatccga tcaaccttcc ggccgcgccc atgtacattg acagcctgat tggtgggcag
    35761 gaactctaca ccggcgtcgt gccgatgatc ggcgataagt ttatccaagt cgtcgcgctg
    35821 gacggcttcc cgatggagtc ctacccgggc attttgacgc ggctcggaga aatgcctatc
    35881 gagtaccggt ggtcgaaccg cttcatcttc atggatcagc atcaggcggt tgccgatttg
    35941 gagaaatacc gcaaaaaatg gaaacagaaa gtacgcggtt tcttcgatca ggtgttcaac
    36001 acccattccg gctccataga ccaagacgct caggcaatgg tggatgacgc ggaagccgcc
    36061 ttggccgaaa ccaaatccgg cttggtggca cagggttact acacaagcgt tgtcattttg
    36121 atgcacgaag accgggcttt cttggaaagc atggcaaggc aggtggaaaa agagatcaac
    36181 actttgggct tcaatgcccg cgttgagacg gtcaatacga tggaagcatg gttgggcagc
    36241 ctgcccggcc acggaatcga aaacgtccgc cgtcctttac tgaatacgct gaacttcgcc
    36301 gacatgattc cgaccagcag catttggacg ggcttggagg aaaacccctg ccagttctat
    36361 ccgcccaatt cgccgccgct gatgcaggtt gttaccagtg gctccagccc gttccgtctc
    36421 ggtctgcacg tcggcgacgt cggccacact ttggttttcg ggccgactgg cgcaggtaaa
    36481 tctaccctgt tggccacttt ggccgctcag gccgaccgtt accggaacat gcgggtattt
    36541 tctttcgaca aaggccgttc tttggaaacg ctgacccgcg ccgtcggagg atcacactac
    36601 aacattggcg atgaagacaa cacggcttcc ggtttgtctt tcgcgcccat gcagcttatc
    36661 caaaacacgt ccgaccgcgc ttgggctttg tcttgggtgg aagcggttat ggaattgaac
    36721 ggcctcaagg ttacgcccca acagcgtaac gaaattgaaa ccgccatcaa aaacaatgca
    36781 gccgaacagt cctattcgct ttccgatctt tcaaacaccc tgcaagacgt agagatgcgc
    36841 gaagtattga aagcctacac catcgacggc tcgatgggtt atctgctcga tgccgaacag
    36901 gacagcttga gcttctcccg cttcacctgc tttgagatcg aagagttaat gaacctgccg
    36961 gaaaaatatg cgcttccgat tatgctgtat ctgttccgca gaatcgaaaa agccctgacc
    37021 ggcgatcccg cagtcctgtt ccttgacgaa gcatggatca tgttggggca tcctgtcttc
    37081 cgggaaaaaa tccgcgaatg gctgaaagtg ctgcgtaaga aaaactgcct tgttctgctc
    37141 gcgacccaaa gcctcagtga tgcggccaac tccggcatcc tcgatgtcct gaacgaatcc
    37201 tgtccgacca aaatctacct gcccaacgcc cacgctaggg aagaggacgc ttcggaactg
    37261 tacaggcgca tgggcttgaa tgcgcggcag attgagattt tggccaccgc cattcccaag
    37321 cggcagtatt actacacctc gccttacggc cgcaggctgt ttgaactggc tcttgacccg
    37381 ctgaccttgg cctttgtcgc cgtatccgac cccgacaccc tgaatgcaat gcggcagacg
    37441 gccgaccaat acggggaaga ctggccgatc cgctggctgc aaaaccgggg aatcaatatc
    37501 cataactatg tcgatgtgga caaatacaga aggaaacact gatgggactg aaagacattt
    37561 ttcgcgtcag ggaaaaagcc gaccgcgaaa tgaccgaaaa cccctatttg aacgccagga
    37621 aacaatggaa cttcatcatg ggcgacatgg tttcgtcgcg cctgctgtgg caatgggcgg
    37681 gcatgaccgg cctgctgatt ggtttgggcg gggtaggggg catgatttac ctcggcagcc
    37741 agtcgaaatt tatcccttat atcgtcgaag tgaacaaatt gggcgaaacc gtggccgtgg
    37801 ccagagccga ccagcctcag gcaaccgatg agcggattgt ccgcgccatg ctcggcagct
    37861 tcatcggcaa tgcccgtacc gtaaccaccg atatagccct gcaacgcaaa tacgttctca
    37921 atacctatgc catgctgaat ccgaaagacg cggccgcagc caaaatgaac gaatggctga
    37981 acggaacgcc ggagtcttcg ccgttcaaac gcgcggaaaa agaactggtc agcgtggaaa
    38041 tcaagtcggt gctgagacaa agcgacaact cttggcaggt ggactgggtg gaaaccactc
    38101 gcgcccatga cggcaaatta ctcaaaccgc ctcagaatat gcgtgcgctg ttgaatatct
    38161 actacgttcc gccgacaaca gagcagcaga ttattgtgaa ccccatcggc atgtttatcc
    38221 gtgatttctc ttggtctaac gaactgtaac aaggtacagc aatgaaaaga cagattttat
    38281 ccttggcgtt aatcaccgct ttgtccactt ccgcctttgc cgacagctac gccgacaaat
    38341 attttgaagg cagccacaac ccgaagctga accgccacga acaaaaaggc ttgggcatcg
    38401 ccaaaaacta ctcttctgac ggcaggatgg ccgtaccgcc cgttcccggc gataacggcc
    38461 gcatcacctt tgccttcggc tctgatccgt ctgtcgtctg tgccgtaatg caggtctgcg
    38521 acattgcctt acagcccggc gaaatgatta acgatctgca cgccggggat accgcacgct
    38581 ggaatatcac tccggcaatt tccggcagca acggcaacga aagaatgcac atcttggtta
    38641 aaccactgga tgtcggtttg caaacgacgc ttttcatagc caccgaccgc agaacctatc
    38701 atatccgcct caaatcacac cgtacccgtt atatgcctgc cgtcggtttt tcgtatcccg
    38761 aagacaccgc cgcgcgttgg cgggcgatcc gggcaaacca agcccggcaa gccgaagctc
    38821 aggttctgcc caccggagaa aacatccaaa acttggattt caactacacc atcagcggcc
    38881 acaatccgtc atggaaacct ttgcgcgttt ataacgacgg cacccgcacc atcatccaaa
    38941 tgccgccgac tttatcccaa ggggaagctc cggccttgct ggtcgtccgt aacggcgaaa
    39001 acaccttggt caattaccgg cttcacgaag accgccacat cgttgacagc ctgtttgacg
    39061 aagccatcat gatcaccggc gttggccgcc atcaaaccaa agtcgttatc agacggagcc
    39121 gatcatgaaa tcaaatgtcc tgttcgtcgc gctggctttg gccgttggcg gatgcgccac
    39181 cgggcaatac ggttctttcg tccgttcaga cgccgtatcc gcctcagtaa ctcaagatgt
    39241 agtggaaaaa ctccaagaag aatttccggc ggccaacacc gttttcttcc tgaaccgcag
    39301 caaatccgat accttcggta cgcaactgga tcaggcattg cgtgaaaaag gctttgccac
    39361 aaccgcccaa cagggcgaaa acgaaaaatc actgaattac gtcttggatc aggttgattc
    39421 cgatatttac cgggtaatga tctacattga cggcacgccc tattcccgtg cctaccgcca
    39481 aaccgaaaac ggcgccgtgg ttccgaccgg ttcttggact aaacgtggag actgaagcat
    39541 gagccaaagc accgaagatc tgctgaacgc atcccgttcc ccttcgatcg caccggatac
    39601 ttccggccgt cgtcccggtg tgcgccgcct gaacaacatt ccgatctata tcgtcggcgg
    39661 tgttgttctc ggattcgccc tgctgattgc actggtggca gcaactaaag gccaacagtc
    39721 tgccgggcag gcaacgcagg gagacggtaa ggatgcaaca agaatggccg attccctatt
    39781 gaacgaactg cctcaggaag gcggtctgat taccgccaaa gcatccgaac cggccatgcc
    39841 cgccctgcct gacggcatgg cgtccgcgcc tgatttaccg gcatccgcgc ccacatcttt
    39901 accgatggtt cccgatatgg ccaaccttcc ggccacacct cccgaagtgg atcaggaagc
    39961 ggaacaaatc cggcaggcaa aagcgcagat gttccaagca gccctagctg ccccaaccaa
    40021 agtggatgtc gtcgcatcga cttctcgggg ttcgtccggc ctatatactg ctgccttatc
    40081 ttcttccggc tcaggtacac ccaaccgtag cgagatgctg gctcaaatcg ccgaagtgca
    40141 gcgcatgtcc gagaatgccg gttccaatcc cgccgaagtg gagcgcgttt acaaccaaca
    40201 actggccaat gcccaacaga tggccgccgc aggtggtcag gacggcgtac aaggggcagg
    40261 cggcatgccc ggcctgaaca cacctccgac tcccggcaat atcccgagac aaacggcaca
    40321 aaacggtgga aacaactggc ggcttgagag ccaagtcgaa cgtccgaacg cctacatgat
    40381 ccgcagcggt gcggttatcc ctgcaaccct gatcagcggc atcaattccg atctgcccgg
    40441 catggttcag gcacagatct cacaaaacgt gtatgacacc gccaccggcc gtcatctgct
    40501 tttaccccaa ggatcgcgct tggtcggcac ttacagcagc ggcatcaatc ttggccagcg
    40561 tcgcctgttt atcgcgtgga accgcattat tttccctgac ggtaaagcct tggacatcgg
    40621 cgatatgccc ggagccagcg gcgcaggcta cgccggattc agcgaccgca tcaacaacca
    40681 ttacggccgt ctgtggggca atgccctgat gctctcccta gtcggagcca gcatcactta
    40741 cggcgtggac aaaaacagta acaacaataa caacggcaat aacaacggca acaactccac
    40801 cgtgcgcgga gccttgtcgg agtccttggg tcagaccttc ggacaggccg tgtcccaaag
    40861 tattcagaaa aacatgaatg tttccccgac gttagagatc cgcccggggt accgcttcaa
    40921 tgtcattctt actaaagaca ttgatttctc taagccctat cgttcctttg actattaaca
    40981 ggagagcatt atgcaaaaac gtcttttaag caaaaccaag accgtagccc tgtctttggc
    41041 cgtttccgtg tccttggggc tgtccgcacc agcatacgca ggcatcccgg taacggatgg
    41101 cggcaacatt gcccaaagtt cggtctccgc cattcaaaat gtggcgcaaa ccctgaagca
    41161 gattgaggag taccgaatcc aactgaaaca gtacgaaacc cagttgcgga actccttggc
    41221 tccggcggct tatgtttggg atcaggccaa caaaactatg gccaacatga aggccgctgt
    41281 cgcccgcttg cgagcctaca aacagcaact gggtaacatg gaagagtacc tgagcaaatt
    41341 ccaagccgtg gacttctaca aaagctctcc gtgctttacc gccaaaggtt gctctgcggc
    41401 cgaccgcgcc aaactcgagc aaaactacgc ccaattgtcg cgcatgcaga aattatctaa
    41461 tgacggccta ttaaggagca tcaataacga gatcgactcc atcagcagag acgccaacac
    41521 cttaacccaa ctccaacgaa acgcccaatc agccaccggc cagttggagg cactacaata
    41581 cgcgaaccag ttccatagcg ctcaagcgca tcaactgctt cagttgcgta atatgattaa
    41641 tactgagatg caggcaaatg ctgcttatcg tcaaagtcaa cttgatagag aggctagata
    41701 tgaagctgct cgtaaacaag ttaggacgtc ggatattttg aaacaccctt atagaagagg
    41761 gcaataaaaa tagggtttct aatgtgtaat aaacattaga aacccactct aaatttgaaa
    41821 gattacctca tgagaacttt attttttgta ttgctaagta gtttcatgct tgttgcgtgc
    41881 caacaaaagg atgagaacca acaacgcaga gaagaactac gtaaagtaga tcaggatgtc
    41941 ctaaaacggc catacaaaag gaaagagtag ttattttctt atgcaatata taacccaaat
    42001 tatg
//
LOCUS       DAXDXW010000005        40702 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXDXW010000005 DAXDXW010000000
VERSION     DAXDXW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40702
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.005"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            203..352
                     /locus_tag="ACFB64_000211"
     CDS             203..352
                     /locus_tag="ACFB64_000211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208667.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
     gene            complement(349..1968)
                     /gene="mnmC"
                     /locus_tag="ACFB64_000212"
     CDS             complement(349..1968)
                     /gene="mnmC"
                     /locus_tag="ACFB64_000212"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC1208668.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDSECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKSDKPWFALPQTSERKKPEHVLVIGAGIAGASTAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTGQTELLLAGYGYTKRLLGHILPDSDTWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLNTPYAEPLCGLYWQHGVW
                     LNPPAFVRTLLSHPLIELYENTTLTGISHDGEKWIASTPNGTFTATHIIYCTGAHSPC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SNTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKHQGHAAIRCDSPDHLPVVGAL
                     GDIAAMRQTYAKLALDKNYRIDTTCPYLPNAYTNTAHGTRGLATAPICAAAVAAEILG
                     LPHLFSQRLRHALHPNRTVIRAIVRRQNLIP"
     gene            2088..2456
                     /locus_tag="ACFB64_000213"
     CDS             2088..2456
                     /locus_tag="ACFB64_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFC1208669.1"
                     /translation="MNNIPVEIRLLKNRAVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            2499..3236
                     /gene="ubiE"
                     /locus_tag="ACFB64_000214"
     CDS             2499..3236
                     /gene="ubiE"
                     /locus_tag="ACFB64_000214"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC1208670.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTIHTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTYKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            3456..3935
                     /locus_tag="ACFB64_000215"
     CDS             3456..3935
                     /locus_tag="ACFB64_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC1208671.1"
                     /translation="MPESIFKQISSDILRLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVETGILLIEDESRNLRLSWVGSARELDRKDNAYKRALSALSRKL
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
     gene            complement(4063..4557)
                     /gene="folK"
                     /locus_tag="ACFB64_000216"
     CDS             complement(4063..4557)
                     /gene="folK"
                     /locus_tag="ACFB64_000216"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC1208672.1"
                     /translation="MNNRHFAVIALGSNLDNPAQQIRGALDALSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFINAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKYGKVVELSKRLGNQGIRL
                     LPDR"
     gene            complement(4567..4941)
                     /locus_tag="ACFB64_000217"
     CDS             complement(4567..4941)
                     /locus_tag="ACFB64_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC1208673.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLFAYIALGMVMMRARPRSTKFYTV
                     YLLAMCCIACIVYLAKTKVLPF"
     gene            complement(4995..5561)
                     /locus_tag="ACFB64_000218"
                     /pseudo
     CDS             complement(4995..5561)
                     /locus_tag="ACFB64_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(5646..5939)
                     /gene="hfq"
                     /locus_tag="ACFB64_000219"
     CDS             complement(5646..5939)
                     /gene="hfq"
                     /locus_tag="ACFB64_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC1208674.1"
                     /translation="MTAKGQMLQDPFLNALRKERVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENKPQAAPASTLVQVETVQQPAE"
     gene            complement(6068..7006)
                     /locus_tag="ACFB64_000220"
     CDS             complement(6068..7006)
                     /locus_tag="ACFB64_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC1208675.1"
                     /translation="MSIRTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIYPADTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRLKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            7327..7767
                     /locus_tag="ACFB64_000221"
     CDS             7327..7767
                     /locus_tag="ACFB64_000221"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC1208676.1"
                     /translation="MDMKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIVREWRKVKVAGHAQEVLETLSR"
     gene            7819..8694
                     /gene="xerD"
                     /locus_tag="ACFB64_000222"
     CDS             7819..8694
                     /gene="xerD"
                     /locus_tag="ACFB64_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC1208677.1"
                     /translation="MENGLIDRLLETLWLDRRLSRNTLDSYRRDLEKIARRLSLCGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYVWMEREGMRADNPTRLLKPPKIDR
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITTLGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRSCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVRLHSVVKEHHSRN"
     gene            8866..9066
                     /locus_tag="ACFB64_000223"
     CDS             8866..9066
                     /locus_tag="ACFB64_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFC1208678.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            9080..9214
                     /locus_tag="ACFB64_000224"
     CDS             9080..9214
                     /locus_tag="ACFB64_000224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208679.1"
                     /translation="MPSELFRRHFCCFWQDLSIIFLEDIVFSQIDHDSAASKALACAN
                     "
     gene            9232..9465
                     /locus_tag="ACFB64_000225"
     CDS             9232..9465
                     /locus_tag="ACFB64_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC1208680.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQDEYGFGLEVVDVDENPVLE
                     EKYNELIPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(9836..10699)
                     /gene="rfbD"
                     /locus_tag="ACFB64_000226"
     CDS             complement(9836..10699)
                     /gene="rfbD"
                     /locus_tag="ACFB64_000226"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC1208681.1"
                     /translation="MRILLTGSKSQLAHCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     VFDGKGKIPHQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYAGGKSVSWYE
                     FARHIFQTALQQDASFPVPELKAVSDEGSAAAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            10892..11755
                     /locus_tag="ACFB64_000227"
     CDS             10892..11755
                     /locus_tag="ACFB64_000227"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC1208682.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDAPI
                     PGKGEILTQISNFWFKKLAHIMPNHFTGDTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMREAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRDKAVRLYTEAAEYAKSRGIIICDTKFEFGLDEEGTLTLMDE
                     VLTPDSSRFWPADQYEVGINPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIRKTVEK
                     YQEALTLLTQD"
     gene            11989..14112
                     /gene="pnp"
                     /locus_tag="ACFB64_000228"
     CDS             11989..14112
                     /gene="pnp"
                     /locus_tag="ACFB64_000228"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC1208683.1"
                     /translation="MMFNKYVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVFVA
                     VTTNKEVKEGQDFFPLAVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYING
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWN
                     AVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTG
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKISQDKI
                     RDVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEEITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPVREENAAE"
     gene            complement(14185..14853)
                     /locus_tag="ACFB64_000229"
     CDS             complement(14185..14853)
                     /locus_tag="ACFB64_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC1208684.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRISSYTAIGLMLGLIGQLGISLDQTRVLQNILYTASNLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSATTGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(14850..15164)
                     /locus_tag="ACFB64_000230"
     CDS             complement(14850..15164)
                     /locus_tag="ACFB64_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208685.1"
                     /translation="MSRLFLLLFPWATLAACTRNIPASPAVSNCAHLHNRTVLYFDAH
                     GKPADSTAKGGFYRTVEIHDTNRILIQDFNSTGNALVKPYWSDCRKLSVFPDKHLTMS
                     HP"
     gene            15301..16152
                     /gene="dapF"
                     /locus_tag="ACFB64_000231"
     CDS             15301..16152
                     /gene="dapF"
                     /locus_tag="ACFB64_000231"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC1208686.1"
                     /translation="MKTLKFTKMHGLGNDFMVIDAVSQDFTPEDVPIAAWADRFRGVG
                     FDQLLVVGCSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     AKGIIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSRIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            16149..16754
                     /locus_tag="ACFB64_000232"
     CDS             16149..16754
                     /locus_tag="ACFB64_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208687.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIALWLGISVL
                     GVKLMPGMWGMTRAAPLFIPHFYLTLGSIFFFIGYWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGVLAAMLMQVAVTAMLLAEIGR"
     gene            16950..17882
                     /gene="cysK"
                     /locus_tag="ACFB64_000233"
     CDS             16950..17882
                     /gene="cysK"
                     /locus_tag="ACFB64_000233"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC1208688.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTKGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEAAEKAGKINKNTVIVEATSGNTGIGLAMVCAARGYKLAITMPESMSKER
                     KMLLRTFGAELILTPAAEGMAGAIAKAQSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWNDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTEIYDSIAKVPNEAAFETARAMAEKEGILAGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            18191..19099
                     /locus_tag="ACFB64_000234"
                     /pseudo
     CDS             18191..19099
                     /locus_tag="ACFB64_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            19145..19294
                     /locus_tag="ACFB64_000235"
     CDS             19145..19294
                     /locus_tag="ACFB64_000235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208689.1"
                     /translation="MDWQAVKTGKTKHADNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            19518..19625
                     /locus_tag="ACFB64_000236"
                     /pseudo
     CDS             19518..19625
                     /locus_tag="ACFB64_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(19730..20749)
                     /gene="lepB"
                     /locus_tag="ACFB64_000237"
     CDS             complement(19730..20749)
                     /gene="lepB"
                     /locus_tag="ACFB64_000237"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC1208690.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDIVEYRDKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIDQSGLNHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSGDSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(20895..22688)
                     /gene="lepA"
                     /locus_tag="ACFB64_000238"
     CDS             complement(20895..22688)
                     /gene="lepA"
                     /locus_tag="ACFB64_000238"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC1208691.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHKG
                     RELASKMRELIPRQMFDIAVQAAIGSRIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(22830..23531)
                     /locus_tag="ACFB64_000239"
     CDS             complement(22830..23531)
                     /locus_tag="ACFB64_000239"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC1208692.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIRQFAPDCVINTGSAGGLGKGLKVGDVVIGTETEHHD
                     VDVTAFGYARGQVPQLPARFASDGILIETAKRAARTFEGAEVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFEEFLKTA
                     AASSAKMVAEIVKSL"
     gene            23699..24829
                     /locus_tag="ACFB64_000240"
     CDS             23699..24829
                     /locus_tag="ACFB64_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC1208693.1"
                     /translation="MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPALKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGEISLQDALKNADSAHDLRLAVQLRSRRAQSSDPDLELL"
     gene            24863..25840
                     /locus_tag="ACFB64_000241"
     CDS             24863..25840
                     /locus_tag="ACFB64_000241"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC1208694.1"
                     /translation="MIYPWHQEQWRQIAEHWTSRPNAWLFVGKKGTGKTAFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGRGSHPDFYEITPLADEPENGRKLLRIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKKVLPAPSHGEALAYLRDRGVAEPEERLAFHSGAPLFQEEGELRELRAKLLEI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENR"
     gene            25844..26194
                     /locus_tag="ACFB64_000242"
     CDS             25844..26194
                     /locus_tag="ACFB64_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC1208695.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTDDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPASTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGSTISGSRPTFTM"
     gene            26199..26978
                     /locus_tag="ACFB64_000243"
     CDS             26199..26978
                     /locus_tag="ACFB64_000243"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC1208696.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAVAEAHEHIYCTIGVHPDSKEAEEFSIAEMVEAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLAILKECKTNSGVIHCFSEDVGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRVV"
     gene            27004..27219
                     /locus_tag="ACFB64_000244"
     CDS             27004..27219
                     /locus_tag="ACFB64_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFC1208697.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYCRSQH"
     gene            27320..27631
                     /gene="grxD"
                     /locus_tag="ACFB64_000245"
     CDS             27320..27631
                     /gene="grxD"
                     /locus_tag="ACFB64_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC1208698.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVAVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            27737..28363
                     /gene="upp"
                     /locus_tag="ACFB64_000246"
     CDS             27737..28363
                     /gene="upp"
                     /locus_tag="ACFB64_000246"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC1208699.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLTTELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPISYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKEKGCRNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSRLNENGYIIPGLGDAGDKIFGTR"
     gene            28386..28706
                     /locus_tag="ACFB64_000247"
     CDS             28386..28706
                     /locus_tag="ACFB64_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC1208700.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDAEGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLGKEAAEQGLIWFAEHVADARAHPGKHPNIDLLENVVQSGETLLLKP
                     LAAQ"
     gene            28875..29516
                     /locus_tag="ACFB64_000248"
                     /pseudo
     CDS             28875..29516
                     /locus_tag="ACFB64_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            29546..30370
                     /locus_tag="ACFB64_000249"
     CDS             29546..30370
                     /locus_tag="ACFB64_000249"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC1208701.1"
                     /translation="MFALAASNMILRGDGKANLHQSSCFMTDFQDLIKNPKPETGLKR
                     PNVGFLNPPYAQSKSDAELHELYFVKEMLDMLAEGGTGIAIIPVSCVIAPSKAKSEIV
                     KYHRLKAVMSMPSELFYPVGTVTCIVVFEAHKPHFQTVVIDPDTQEEISTKKACRKTW
                     FGYWRDDGFEKTKHLGRIDLYDRWQGIKARWLEHYLNNEVHTGESVTAFVTDNDEWVA
                     EAYLETDYSKITRADFEQVVREFALFQLLGAEVGPTENLDNESYEDDDNNDFGDDE"
     gene            30370..31338
                     /locus_tag="ACFB64_000250"
     CDS             30370..31338
                     /locus_tag="ACFB64_000250"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC1208702.1"
                     /translation="MVELQEIFDVSYGSKLDLNKMSSFNPTINFVGRSGKNNGVTASV
                     DLLKNTKPYPAGLLTVALGGSVLSTFLQNKPFYTAQNVAVLNPKTEMTEQQKLFYCAA
                     IFANAYRFSACGREANRTLRQLFVPSLDEIPSWVESVNLNPSAGVTEPKLKEPLDLPV
                     VRQSKRLDEIFTIQNGIAATKLKEFEQRQKDTVVYIRPASTQARTLRSYIARDSVDEK
                     HIFPCHTLFTSTNGEGSHTYSYVSTCEFVANSDVAVLTPIQSDMPIEVKLYYAKCITA
                     NRYLFSYGRKPKGEKLKSIMLPYFDQQEDFDYICRFIHTLLFSNNL"
     gene            31620..32042
                     /locus_tag="ACFB64_000251"
     CDS             31620..32042
                     /locus_tag="ACFB64_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC1208703.1"
                     /translation="MKKMFLSAALLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFSR"
     gene            32060..32824
                     /locus_tag="ACFB64_000252"
     CDS             32060..32824
                     /locus_tag="ACFB64_000252"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC1208704.1"
                     /translation="METAKPVMLIVRPSGRAKDDVEVCRRAGWQAEVLSPIEIETDEA
                     SLKRLPEMYARADAVFWVSPAAVETAVPYLNLSDGIKAHIAVGQGSRRALARYAGAGV
                     FAPEDGNDSEAVLRLPVWNSLPEGARVLSVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVQSLFAQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSYSSISVSDGMVFPGTSNQ"
     gene            32836..34170
                     /locus_tag="ACFB64_000253"
     CDS             32836..34170
                     /locus_tag="ACFB64_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC1208705.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAACALVLAALGLGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQSAIQSELDRLDSGVKANGEQILMTQKAYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNGEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKTLDVRMTADDGLKTSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKAIEAPSLPSERKPEQPAKKQPAPEKAGRSPSAKGERA"
     gene            34167..35384
                     /locus_tag="ACFB64_000254"
     CDS             34167..35384
                     /locus_tag="ACFB64_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFC1208706.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPENMRRSGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQMADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADSWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSIPARLVLAKVFDETAQSQKAEAQRNLVLA
                     SVAGENRPSAETR"
     gene            35459..36523
                     /gene="hemE"
                     /locus_tag="ACFB64_000255"
     CDS             35459..36523
                     /gene="hemE"
                     /locus_tag="ACFB64_000255"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC1208707.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTGLATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFAHA
                     LQHESDIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLYKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMVQIGADALGLDWTCNIGE
                     ARRRVGNQVALQGNFDPSALFGTPESIRTEVARILTGYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(36586..38460)
                     /locus_tag="ACFB64_000256"
     CDS             complement(36586..38460)
                     /locus_tag="ACFB64_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HFC1208708.1"
                     /translation="MNQLTPPELADSVKNTVDGFMEKLSQTEPKIAQNVLLLGNVQSG
                     KTAQVLGVLSALADDGDHKVFLYLTTDSVDLQDQTVKRAKANLKNFIVLSEADDRSFM
                     EVMKAENPILVVIKKNARVLKRWRNLFASQSSLKGYPLVIVDDEADAASLNTNSDKPA
                     KDASTINKLLNDIKNSCCQSLFIQLTATPQSLLLQHEESDWQPEFIHFFEAGEKYIGG
                     NFVFSDPPSYIVRFIDSELDDMKDESGEIAEGAKQALLSFLITCAEFALCDKANCNFA
                     LHPSYKIQDHQAFSKKIQAFLNDLVQAVNNGEDLAGSFKESYLDLQKTKPDIHHFDEI
                     YEKLTALLENKQISTLVVNSQTETDFDLEKGFNIIIGGNVIGRGLTIPKLQTVYYSRT
                     AKKPNADTFWQHSRIFGYDRDKSLLRLYIPFDVYYFFVQLNQANNLIIGQAKNSGGNI
                     QVIYPKNINPTRKNVLKFDSINQIVGGVNYFPLHPNEDNLSEINKILPSILKDEIQSD
                     LYQIDIEDLFLVLDKLGRYVPDDWNKEKFIAGVEALKAQRPSFKTYVLIKTGRKLSRA
                     TGTMLSEDDRKLGEKYPNDLFLTLYQVVGNKDKGWQGKDFWLPNIKLPHNGLVYQSAK
                     "
     gene            complement(38476..39513)
                     /locus_tag="ACFB64_000257"
     CDS             complement(38476..39513)
                     /locus_tag="ACFB64_000257"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease PLD domain-containing
                     protein"
                     /protein_id="HFC1208709.1"
                     /translation="MNTVFSNIANAKITEKSLNAVWMDLFKSADEVLMATGYVSNDAV
                     VELHKILELNDHIQKIDLLVGMHYLEGFSHLQYDSLCKLNDFLRHEKRGAVYVSPFVK
                     FHGKMYSFKNYQKINGLIGSANLTCFWDSTERTYETMLHLNGKPAQILQADIQSTIHK
                     LGKNIQEVERPSKFIEHNSHLENCLGVQKIAPEQIRQLFAQTSEYHFSIPAKTEEKSN
                     LNVFFGEGRRDKRGFVKPRPWYEVELIVSKDITSQEGYPVLKSFTVITDDGWQFQCKT
                     SGDYSKNFRSENDLKTLGKWIKGRLESHGCLQNNEKITHETLREYGNDHFELRSTDNP
                     DVWLLSFKGKN"
     gene            complement(39510..40634)
                     /locus_tag="ACFB64_000258"
     CDS             complement(39510..40634)
                     /locus_tag="ACFB64_000258"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1208710.1"
                     /translation="MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGCETVWAND
                     FSHWACESFRKNIGDVIVEGDIEQINPNDPTIPDCDIILGGFPCQDFSMIWKQPGLEG
                     ERGNLYKSFLRFVNAKKPKVFVAENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYN
                     FAEFGVPQFRERVLIVGVRLDTGFDFRHPEPTHNETGENGLKPYVTAGQAISNIPQNA
                     SNNELLKISDKTRRMLELIPEGGNFTDIPKDHPLYVKGMISHVYRRMHRNEPSKTIIA
                     AGGGGTWGYHFPEPRAFTNRERARLQSFPDDFEFVGSTTEVRRQIGNAVPPQGVVELA
                     KSILPIFSDNYEKVDLHEKLVEEKEILFHDRLSKIRGGKQ"
BASE COUNT        10056 a         9354 c        10953 g        10339 t
ORIGIN      
        1 ctattgagtc ctcgagaagg gaaataaaaa ttaacatcct tatatattga gttcctgaga
       61 agggaagatt aacaaaaatt aacgcccttt gcttcataca agtaacaggg ctttttcatc
      121 ccttccttat ctaacagggg gtacagaaac cgaagcggct ggcagggtta aggaagtctt
      181 cgaatgttac ggaaaattca tcttggacag caaaggcaat ttgttaggca ttccttactc
      241 cttattttgg gaagaaaacg ttatgggtgt tttcgatatt ttaccgtcag gattggtatg
      301 tttatttgaa tatgattttt tgtggtcggg acggcatgcg gcaaaggctt aaggaatcag
      361 gttctgcctt ctgacgatgg cgcggatgac ggtgcggttg gggtgtaggg cgtggcgcag
      421 gcgttgtgaa aagagatggg gcaagcctag gatttcggcg gcaacggcgg cggcgcagat
      481 gggggcggtg gcaagcccgc gtgtgccgtg ggcggtattg gtgtaggcat tgggcagata
      541 tgggcatgtg gtgtcgatgc ggtagttttt gtccagtgca agtttggcgt aagtctgccg
      601 catggcggca atgtcgccga gtgcgccgac gacggggagg tggtcggggc tgtcgcagcg
      661 tatggcggcg tgcccttggt gtttttgggg gtttgggttg gcggcaaaca atgattcggc
      721 aagggcgggg ttaaggtgtg ccaatgcttg gcggtttgag gcttcttcgg cttcgttcca
      781 tccggtattg ctgctgttgg gaataaaact cgcgccgtag cagtgcagtc cgtgccacga
      841 cgggctgatg tagctttcgc ctgaaacggc gcaacgcagt tgttcggaaa acggggtgga
      901 cggtgtgagt ccggtttgtc cgcgtatttg cctgaggggt agggcggcga ggttggtttc
      961 gggcaggcag gggctgtgcg cgccggtgca gtagatgatg tgtgtggcgg taaatgtgcc
     1021 gtttggcgtg cttgcaatcc acttttctcc gtcgtgggaa atgccggtta acgttgtgtt
     1081 ttcatatagt tcgatcagcg gatggctgag gagggtgcgg acgaatgcgg gcggattgag
     1141 ccatacgccg tgttgccagt agagtccgca taatggttcg gcgtagggcg tgttcagcgg
     1201 gatgccggcg attttttcgg cttctgcaga cgtgatgctg cggtagaggt ggttatggtg
     1261 tttttgcaaa cccaattcgt gattgcgttg ttgttcggtg cggctgtaat tgaggtggat
     1321 gatgccgttg ccgccccaag tgtcggagtc gggcaggatg tgtccgagca ggcgtttggt
     1381 gtagccgtag ccggcaagca gcagttcggt ctgtccggtg tcgtgcggcg agattttggc
     1441 gtaaagcagc ccttgccggt tgccgctggc ggcttgagcg gcttttcggg cttccaatac
     1501 ggtaacggaa atgccgtgtg atgctaaggc gtgggcggtc gatgcgccgg caatgcctgc
     1561 accgatgacg aggacgtgtt ccggtttttt ccgttcggat gtttgtggaa gtgcaaacca
     1621 gggtttgtcg gacttgcttt cggtttgcgg gatggcttcg gtctgccagg aaatgtggtg
     1681 gaagtgttcg tgcaggcggc gggtgcgtga agggggaacc agccagaagc ggacatcggg
     1741 caggatgtgt tcgatgaggt tgatgctgtc gaactggagg cactgggttg cctgatccaa
     1801 acggtgcttc agacggcatt ccgagtccga agcatcttgt gcggtttgaa aatcgggaat
     1861 ctgattatcg gggaggcaga caatcaggtt gagcgggtgt gcgtgtttgc ggatggtttg
     1921 gtcgagtgtg cggatgtcgg gaatgccgtc ccatacgaga ttgtccatat caatgccgtc
     1981 tgaagtgtgg gtttgaatat cggtatcggg ataaagctgt taaaatacgc gccgtttaaa
     2041 ggcacgcgta cgcctgccgg atattgtatg ccgaaccgag gtgttttttg aataatattc
     2101 ctgttgaaat ccgtttgttg aaaaaccgtg ccgtgttggt tttgacttat ggggacgaac
     2161 ctaaaaatct gcctgccgaa tttttacgcg tctattcgcc gagtgcggaa gtgcgcggac
     2221 acggcgtggg acaggatgtt ttgcagaccg gtaaggcgga tgtgcagatt acggatttgc
     2281 agcctgtcgg acagtatgcg ctgaaaatca gtttttcgga cgggcacgac agcggtcttt
     2341 acgattgggc gtatctgcac agactggcat acggatacga tgcgatgtgg caggaatatt
     2401 tggacaaatt ggcggcggcg ggcgcgtcgc gttttgaaaa gcaatcagat tgttaagttt
     2461 gggatgccgt agggcggtat cagggaggtg gttagaatat gggcggacaa aaaacgcatt
     2521 tcgggttcag cacggtcaac gaagatgaga aagccggaaa agtggcggaa gtgtttcact
     2581 ccgtcgccaa aaactacgac attatgaatg atgtgatgtc ggcagggctg caccgcgtat
     2641 ggaagcattt taccatccat acggcgcacc tgaaaaaagg cgataaagtg ttggacattg
     2701 cgggcggtac gggcgatttg tcgcgcggtt gggcgaaacg ggtcggcaag gaaggcgagg
     2761 tttggctgac cgatattaat tcttctatgc tgaccgtcgg gcgcgaccgt ctgttgaacg
     2821 aaggcatgat tttacccgtg tcgcttgccg atgcggaaaa actgcctttc cccgacaatt
     2881 atttcaactt ggtctccgtg gcgttcggct tgcggaacat gacgtataaa gatgccgcgc
     2941 ttaaggaaat gtaccgtgtt ttgaaaccgg gcggtacgtt gctggtgttg gagttttcca
     3001 aaatctacaa gccgctcgaa ggcgcatacg atttttattc tttcaagctg ttgccggtaa
     3061 tgggcaggct gattgccaaa gatgcggaca gctaccagta tctcgccgaa tccatccgta
     3121 tgcaccccga tcaggaaact ttgaaacaga tgatgctgga tgcaggcttc gacagcgtgg
     3181 attatcacaa tatgagtgcg ggcatcgtcg cgctgcataa gggcgtgaaa ttttaaacgg
     3241 actgcctgtg aagccaatgc cgtctgaaca cgtttcagac ggcatttttg tattttttga
     3301 gacaaaggtt ttaaatctta aaaattaatt catatattta tcaataattt ataaactttt
     3361 taaaaaatag gaacaattat catttgtaaa attgggagat gtctgtataa tgcagtcaat
     3421 ccagtaaaca acgcagcaga cgaaaggagg gaaaaatgcc ggaaagtatt ttcaaacaga
     3481 tttcctccga tattttgagg ctgcaccgtg attctgttta ttcgttgctt gccacttccg
     3541 gctgcaactg tcaggtgcat gaagcggcat atgtcaatat tgacggcaaa tattatattg
     3601 cgctttcgtg cgaacccgag gtgggggaag ttgaaacagg tattttgttg attgaagatg
     3661 aaagccgcaa ccttcgtttg agctgggtcg gtagtgcgag ggagctcgac cgcaaggata
     3721 atgcctacaa acgcgccctg tccgcgttgt ccagaaagct ggggcggtgt aaggacaagc
     3781 tgcatacggc ggttcagccg tttttgttgg agctggtgcc ggagaaaggc agattttctg
     3841 tcggtgatga agaagtttgg atttctcgaa acgatttagt gagggcctta tatcctgtcg
     3901 ggtacagtat gcggcaggca gtgttgcaga tttaaagttt tggtagtggt ttgtgttcct
     3961 tttgcgcccc tttctcaagg ggcgattttt tttgcacgcg tgttggcggc aaaggaaaaa
     4021 tgccgtctga aaccggtgtt cggacggcat ccgcgtgcgg aattacctgt ccggtaaaag
     4081 acggatgcct tgattgccca gccgttttga caattcgaca acctttccgt attttcccaa
     4141 aataaaatca gggaggattt ctgccaaagg gcgtatgacg aaactgcgtt cgtgcgcgcg
     4201 cggatgcggc agggtaaggc gggggtcgtc gctggagatg ccgtcaaagt cgataatgtc
     4261 caaatccaat gtgcgcggtg cattgcggaa actgcgttcg cgtccgaaat cggcttcgat
     4321 acggttgagt tcggcaagca gggcaatgcc gtccaaggtg gtggaaacgg tgcagacggc
     4381 attgatgaaa tcgggctgat tgtcgtaacc gacgggtgcg gtcatataca gtgaggaagc
     4441 ctgttcaagc cggatgtcag gatgggacga gagcgcgtct aatgcgccgc gtatttgttg
     4501 tgcggggttg tcaaggttgc tgcccaaggc gatgacggca aaatgtctgt tgttcatggc
     4561 agtgtttcag aatggcagga ctttggtttt ggcaaggtaa acgatgcagg cgatgcaaca
     4621 catagcgagc aggtaaacgg tgtagaactt ggtcgaacgc ggacgggcgc gcatcattac
     4681 catgcccagt gcgatgtagg cgaacaggag caggattttt gtgccgagcc aaggcgcgtt
     4741 gaacggggag aaatgggtaa tcttcatcag ccacaatccc gtaaacagca gcatcgtgtc
     4801 gttgaggtgg ggcagtgctt tccaaaagcc gaccaagggc ttttctggat ttttccaaag
     4861 taggaaaaaa cggatgttga ataccaaaat ggtgatggta acgaagattt gatggctgta
     4921 tttgacaatc agatactgca tggtcggctc gtatcaaaat aagggttaga atcggcttat
     4981 tttaccgcaa acagttattt ttgacgcagt ttttcaaatg ccaaaagata gggtgggcta
     5041 ttcttccggt tggtaaagcc gtaacgtaaa acggcaaact gttcttgagg caggtttttt
     5101 gcccattgtc cgacggcttc tgcctcctgt ttgccgtttt cgtgtcccgg atagaggacg
     5161 gcaataagca taccgtcttc tttcagcagg gagagggttg ctcaaagggc gggaatgctg
     5221 gtttccttgt cggtggtcag attcttgtcg cctccgggca accagccgaa attgaaaacg
     5281 gctgcatcca gcggctccgg aatatattgc ttcaggtttt catgtccgtc caagatgagc
     5341 cgtacattgc tgtaacctgc ttcctgcaga cggcatcggg tgttgttcag ggcttgcggc
     5401 tggatgtcga atgcccacac tttccccctg ttgcctgcgg tttgtgcgag gaaaagggtg
     5461 tcgtgtccgt tgccggcggt gccgtccagc gcattgccgc cttcgggaag tgcctcccgc
     5521 aaaaggcaat gggcgaatgg aaggatgttt ttcaataata tttttaaatg ccgcctgaaa
     5581 ataaaaatac cttaccgttg tccggtaagg tattgaaaga tatgacacgt catgcttcgt
     5641 gcggattatt cggcaggctg ctggacggtt tccacttgga caagggtcga agccggtgcg
     5701 gcttggggtt tgttttcatg ttggaggttg acggagcgag ccggtacgat ggtggaaatg
     5761 gcgtgtttgt aaaccatttg ggttacggaa gtgtttctca gaagaacgac gtattggtcg
     5821 aaagattcga cctgaccttg caatttgata ccgttaacta agtaaatcga aaccggaaca
     5881 cgctctttac gcaatgcgtt caggaaggga tcttgcaaca tttgtccttt agctgtcata
     5941 ttttttaact ccgctattat gattgtgaaa tcgggcagat gccctgtgcg tgtcgaattg
     6001 tagcctggaa taggaaaaat taccaatttt tttgtgtttg ggcggttttc cgccgggcat
     6061 ttgtatgtca ggagcgttgc tgcagcatcc acgattcgat tttgcgggcg tcgttgacgc
     6121 gtgtggcgga tgtgggcgag ttcagcaata cgatggtaac gggttggttt tgaatgttgg
     6181 ctttgacaac catagacctg cctgcctcgc ggatgtagcc ggttttctgc aattcgatgt
     6241 tccacatgcc ttccctgacc agggcattgg agtttttata gttttgctgc ccgtttttgg
     6301 tttgtaccga ggcgtagttg gaagtcgagt tggtgcggat ttgcggatat tgggcggcgg
     6361 cgttgaccat gaggctcagg tctttggcgg tggaaacgtt ttggaagttg agtccggtcg
     6421 gttcgtaaaa gcggctgccg tacatgccca ggctttgggc tttgcggttc atggcggcga
     6481 caaatgcgcc catgccgccg gggtaggttc tgcccaatgc atgggtggcg cggttttcgc
     6541 tgctcatcag gctaaggtgc agcagttctt tacgtgtaag tgccgtacct atggcaagac
     6601 ggctgccggt ccctttgagg cggtcgattt cgtcgggtgt gatggtaacg gtttcgttca
     6661 tgtccaagtt tgcatccaaa acgaccatcg cgctcatgag cttggaaatg gaggcaatgg
     6721 gcataatcct gtcggcgttt ttctgataca gtatttgccc tgttttgttg ttgacgacca
     6781 gtgcggattg ggaggacaga atcagcccgc ccgtaatggc ctgggtatcg gcgggatata
     6841 tcgtgctttc ggcgaatatt tctatcggat cggaggaggt aagcatgttc tgttctaaaa
     6901 attgccctaa aatgtcgttg tcggcaaaaa ggtgggctga cggcagggaa aggcagaggc
     6961 cgagcagcag cgatgagggc aggcgtttca gggtgcggat ggacattttt ggttccgtat
     7021 ttttgagtat tggcgttatt ttgttgaaaa aacagccatc tgtaaagtat gtcgtctgac
     7081 aggagactgc ccgtaggcgg gcggtcttgt tgtgtttttg gagttgggtt ttataacatt
     7141 ctgtttttaa atcggaacat attttgtggt ttgacatgga tatttttcat gccgtcgtgt
     7201 gtcggtttgg atgtttccgg cggttgaatc cttgtccttt ggggcggtag aatcggggtt
     7261 ggtttggcaa ttgcggcggt gcgtctgcgt gccgttttga ataatgggaa tatcgggagt
     7321 aggactatgg atatgaaata tgaatttacc ctgccttcga gcagcggtgc ggattttcat
     7381 tcggcagaac atctgccttt ggtcgtgtat ttttatccga aagacagtac gccgggctgt
     7441 acgacggaag gcttggattt taatgcgcgt ttggaacagt ttgaggcatt gggttatacc
     7501 gtggtcggta tttcccgcga cggcgtaaag gcgcatcaga atttttgcgc caagcagggt
     7561 ttccggttcg agctgttgag cgacaaggat gaaacggtgt gccgcctgtt tgatgtcatc
     7621 aaattgaaaa aactgtatgg gaaagagtcg ttaggcatcg agcgcagtac gttcgttctg
     7681 aacaaggacg gcgaaattgt ccgggagtgg cggaaagtca aagtggcggg tcatgcacag
     7741 gaagtgttgg aaacgctttc ccgatgatgt gaaccatgcc gtctgaagaa gattcagacg
     7801 gcattggttt ggaacgttat ggaaaacggt ttgattgaca ggctgctgga aactttgtgg
     7861 ttggatcggc ggctcagtcg gaatactttg gacagctacc ggcgggattt ggaaaagatc
     7921 gcccgccggc tgtctttgtg cggcagaacg ctgaaggatg cggacgaagc agatttggcg
     7981 gcggcggttt atgttgacgg ggagcaacgg agttcgcagg cacgcgcact gtcggcgtgc
     8041 aaacgcctgt atgtgtggat ggaacgggaa gggatgaggg cggacaatcc cacccgcctg
     8101 ctgaaaccgc caaaaatcga cagaaacatc ccgacactga ttacagaaca gcagatttcc
     8161 cgactgcttg ccgcaccgga taccgacacg ccgcacggtt tgcgggataa ggccctgctc
     8221 gaattgatgt acgcgaccgg cttgcgcgtc agcgaggcgg tcgggctgaa tttcggcaat
     8281 gtggatttgg acaggggttg tattaccacg ctgggcaagg gcgacaagca gaggatggtc
     8341 ccgatgggac aggagtcagc gtattgggtg gaacgctatt atacggaggc acgcccgctt
     8401 ctgctgaaag gcaggagttg cgacgcattg tttgtcagtc agaaaaaaac gggcatttcc
     8461 cggcagttgg cgtggatgat tgtcaaagaa tatgccggca gggcaggcat cgggcacatc
     8521 agcccgcaca gcctgcgcca cgcctttgcc acgcatctgg tgcggcacgg attggatttg
     8581 cgcgtagttc aggatatgtt gggacacgcc gatttgaaca cgactcagat ttatacccat
     8641 gttgccaatg tgcggctgca tagcgtggtt aaggaacacc attcccgaaa ctgaggcttc
     8701 tgcttcggtt taataagaaa tcaatcgaaa atgcaaactt gataaaaaat taccaagaca
     8761 aaccgtgtat gccgaccttg caatgcgaac cgtttttatt tatatttgca tacgataata
     8821 aaagccgcta tcggtacgat agtttgagaa cacacggagc acaaaatgtt tgtctgcatc
     8881 tgcaatgccg ttaccgacca tcaaatcaag gaaaccatcg ctgccggcgc gaccacaatg
     8941 ggcgatttgc agtcgcaatt gggcgtggcg agctgctgcg gctgctgcgg ggagcttgcc
     9001 gcttcgtttc tgacggcgca caacgcgcaa ccgacggtta cggcgggtat caacgttcaa
     9061 gcgtaaaacg gttttggaaa tgccgtctga actgttcaga cggcattttt gctgtttttg
     9121 gcaggacttg agtatcatct tcctcgaaga cattgttttt tcccaaatag accatgattc
     9181 tgctgcgtct aaggctttgg cgtgtgcaaa ttgacagata aggaaacgcg gatgaaattg
     9241 accttgatgt ttcgtgaata ttgcagcttg tgccacaaaa tgcgcgatgc actcaaacct
     9301 tttcaggatg aatacgggtt cgggctggaa gtggtcgatg tggatgaaaa tcctgttttg
     9361 gaagaaaaat acaatgagct gattcccgtt ttgttggcgg gagatgagga aatttgccac
     9421 tggtttttgg atgaggacag gttgaaacag tttctcgaac ggtaaaaaat gccgtctgaa
     9481 gcaggacttc agacggcatt ttttcaaatc aacgttcttt acgtttttgt ggggctgatg
     9541 acctgccggt aaaggaagca cgtttggatg cctggtaaat tgccaatctt ctacggtgtt
     9601 gcatattgac cctttcttta ttttatttgt cggttgggag aattcttaat ttattgattt
     9661 tttcaataaa aattagaaaa tttattgtga gatgtcattg ttggcaatca tatcatgttt
     9721 tgctgttgat ggaagcatga ttgtgtaaag atgatatgtg tttgtgtaat cagtagattt
     9781 tataagaaaa ccgccaaggc ggtgcttggc ggaggaaacg gaggggcggg cggcatcaga
     9841 gcagcttgga aacgacctgt gcaagggctt tttgccagtc tgacggtttg atgccgaagt
     9901 cgttttcgat tttgcggcag tccaaaatgc tgtatgcggg cctgggggcg gcagccgcag
     9961 agccttcatc cgaaacggct ttcagttcgg gaacgggaaa agaggcatcc tgttgcaatg
    10021 ccgtctgaaa gatgtgccgg gcaaattcgt accaggatac ggatttgccg ccggcgtagt
    10081 ggtaaatgcc gcgaacggga ttggactgct gcaacaggcg gatgatggcg gcggacaagt
    10141 cgccggcata ggtcgggcag ccgatttggt tgtggacggc ggacagcggg gaacgttccc
    10201 gcgcaaggtt cagcatcgtg cggacaaagt tgtccccgta ttcgctaaac agccaagaag
    10261 tccgcaggat aaggctgtcg ggattggcag acagtgcgag cagttcgccg gcggttttgg
    10321 attgtccgta tacattggaa gggttggtaa agtcgctttc ctgatggggt attttccctt
    10381 ttccgtcaaa gacatagtcg gttgagatgt ggatgaatcg ggcatgggcg cgatgtgctg
    10441 ccaaggcaag gttgtaaacg gcggaagcat tgacggcaaa tgccgctgcc gcatcgcctt
    10501 ccgctttgtc gacggcagta taggcagccg tgttgacaat agcgtcgggt tggaaacttt
    10561 tgaccatgtt gcagacggca tcggcatcgg taatgtctag ggatgcggaa tccgtcgcaa
    10621 tggtttccca gtcttcagga agacggtcgc gcaggcagtg tgccagttgg cttttcgagc
    10681 ctgtcaatag gattctcatg aggtatttcc tttggtgaaa gtgtattgta ggacttgctg
    10741 tcggtattat agtgccaaaa ctttgccgac ggttgacggg ttggcttttt gtgccatggg
    10801 tattgttttg cgccgacttc ggctagaata tcggtttgcg attcaaacct gtcgggtcgg
    10861 tattttcaag atattttcag gggggtgcga aatgagtgag attggtttgg taaagattta
    10921 ttccggaaaa gtgcgcgatt tatatgaaat cgacgataaa cgtatgctga tggtcgcttc
    10981 cgaccgcctg tccgcgtttg atgtgatttt ggacgccccg attccgggta agggcgaaat
    11041 cctgacgcag atttccaatt tttggtttaa aaaactggcg catattatgc ccaaccattt
    11101 caccggcgat accgtgtacg atgttttgcc tgaaaacgaa gccaaagctt tggaaaaacg
    11161 cgccgttgta gctaaaaaac tcactccggt gaaagtggag gccattgtgc gcggttatct
    11221 ggcaggcagc ggttggaaag attatcaaaa aaccggctcg gtttgcggta ttcggctgcc
    11281 cgaaggtatg cgggaagcgc aacaactacc cgaagtgatt tttacgccct caactaaagc
    11341 cgcagtcggc gatcacgatg aaaacatcag ctttgaagaa tgcggacgca ttatcggtaa
    11401 agaattggcg gaagaagtgc gcgacaaagc ggttcggctt tacaccgaag cggcggaata
    11461 tgccaaatcg cgcggcatca tcatttgcga taccaaattt gaattcggtt tggatgaaga
    11521 aggtacgctg acgctgatgg atgaggtatt gactcccgat tcgagccgct tctggccggc
    11581 ggatcaatat gaagtcggca tcaatccgcc gtcttttgac aaacaattcg tccgcgactg
    11641 gctggaacaa agcggttgga acaaaaaagc ccctgcgcct aaagtgcctg ccgatgtaat
    11701 tcggaaaacg gtcgagaaat atcaggaagc attgacttta ctgacacaag attgattttg
    11761 aagactgaag gccgtctgaa aaggatatgt ttcagacggc ctttttattg tatcaatact
    11821 ggattttaag gatggttgcc tttataatcc gcaattgttt tcagcgtccg aaatgccgtc
    11881 tgaaagcttg tttacaacct gccgcacggt ctgaaaccct aactatgcac attcggattt
    11941 tagtgtgcat tattagtgtt ttagcagtgc ggtattttga aaggaacaat gatgttcaat
    12001 aaatacgtta agaccttcca atacggtaat caaaccgtta ctttggaaac cggcgaaatt
    12061 gcccgccaag ctgccgctgc cgttaaagta tcgatgggcg acactgttgt ttttgttgcc
    12121 gttaccacca acaaagaggt caaagaaggt caggacttct tcccgttggc cgtcgattat
    12181 ttggaacgca cttatgccgc aggcaaaatc cccggcggct tcttcaaacg cgaaggcaaa
    12241 caaagcgaaa aagaaatcct gaccagccgt ctgatcgacc gcccgattcg tccgctgttc
    12301 cccgaaggtt tctaccacga catccaaatc gtagcgatgg ttgtgtccgt cgatcccgaa
    12361 atcgattccg acattcctgc aatgttgggc gcgtccgccg cgctggtgtt gagcggcgta
    12421 ccgttcgccg gcccgattgg cgcggcgcgc gtgggttata taaacggcgt gtacgttttg
    12481 aacccgacta aagccgagtt ggcgaaatca caattggact tggtggtcgc cggtacttcc
    12541 aaagccgtgt tgatggtgga atccgaagcc aaaatcctgc ccgaagacgt gatgttgggc
    12601 gcggtggttt acggccacga tcaaatgcag gttgccatca atgcaatcaa tgaatttgcc
    12661 gacgaagtga atccggaagt ttgggattgg aaagcacctg aaaccaatga agaactggtt
    12721 gccaaagtac gcgggattgc cggcgaaacc attaaagaag cgttcaaaat ccgtcaaaaa
    12781 caagcgcgtt ccgccaaatt ggacgaggct tggaatgcgg taaaagaagc cttgattacc
    12841 gaagaaaccg acacgctggc agccaacgaa atcaaaggta ttttcaaaca cttggaagcc
    12901 gatgtcgtcc gcagccaaat tttggacgga caaccgcgca tcgacggccg cgacacccgc
    12961 accgtccgtc cgctgaacat ccaaaccggc gtattgccgc gcacgcacgg ttccgcactg
    13021 tttacccgtg gcgaaaccca agcccttgcc gtagcgactt tgggtacttc gcgcgacgaa
    13081 caaatcatcg acgcgttgtc cggcgaatat accgaccgct ttatgctgca ctacaacttt
    13141 ccgccgtact ctaccggcga agtgggccgc atgggcgcgc cgaaacgccg tgaaatcggt
    13201 cacggccgtt tagctaaacg tgcgttgttg gccgtattgc cgaaacctga agatttcagc
    13261 tacaccatgc gcgtggtctc cgaaattacc gaatccaacg gctcttcctc tatggcttct
    13321 gtctgcggcg gctgtttgag cctgctgtct gccggcgtgc ctttgaaagc acacgttgcc
    13381 ggtatcgcga tgggtctgat tttggaaggc aataagtttg ccgtcctgac cgacattttg
    13441 ggcgacgaag accacttggg cgatatggac tttaaagtgg ccggtacgac cgaaggcgtt
    13501 accgcgctgc aaatggacat caaaatccaa ggcattacca aagagattat gcaaattgct
    13561 ttggcacagg ccaaagaagc gcgtttgcac atcttggatc agatgaaagc tgccgttgcg
    13621 ggtccgcaag agctgtccgc acacgcgccg cgtttgttca ccatgaaaat cagccaagac
    13681 aaaatccgtg atgtgattgg taaaggcggc gaaaccattc gttcgattac cgctgaaacc
    13741 ggtactgaaa tcaatatcgc tgaagacggt accatcacta tcgctgcgac tactcaagaa
    13801 gcgggcgatg cagcgaaaaa acgcatcgaa gaaatcactg ccgaagtgga agtgggcaaa
    13861 gtgtacgaag gtactgtggt taaaatcctc gacaacaacg tcggcgcgat tgtcagcgtg
    13921 atgccgggca aagacggttt ggtacacatc agccaaatcg cacacgagcg cgtacgcaat
    13981 gtcggcgatt acctgcaagt cggtcaggtg gtgaacgtga aagcattgga agtggatgac
    14041 agaggccgtg tccgtctgtc catcaaagcc ctgctggatg cgcctgtccg tgaagaaaat
    14101 gccgccgagt aacgcttagg gtgaaaatac cgtctgaaca ggtttcagac ggtatttttt
    14161 acgggtatcg ggaatgaatg gggcttacag ccacaggacg gcaagcttcc ataatgccca
    14221 taatgatacg gataatcctg tacacaggcg gatatatcgg ttttgcatga tttttttcag
    14281 ttgcagggaa aaaatgccga ttgccaaaag attgggcagc gtacccagtg caaaggcaag
    14341 catatacagt ccgccggttg tcgcactacc gcttcccagc gcgtaaagtg atgcgctgta
    14401 aaccagtccg cacggcagcc agccccataa tattccgaca gcaaggcagg cgggtatgga
    14461 ttttatgggc agcagccggt tgagtatcgg gttcaggttg cgccatatcg gtttgccgat
    14521 tttctcgatt tttgccgcca aggaagaaat accgctcaag tataagccta aaaagagcag
    14581 caggaggttg gaggctgtgt ataaaatatt ttgcaggacg cgggtttggt cgagtgaaat
    14641 gccgagttgt ccgattaatc cgagcatcag accgattgcc gtatagctgc ttatccgtcc
    14701 tgtgttaagc agcagaatca gccaaaagcg gttgatatgc ggggggagtt ggagcgcaaa
    14761 cgcgctgctt aatccgccgc acataccgat gcagtgagtt ccgccgaaga aaccgagcag
    14821 gaacagggtg aggaaagtga tgtcgtggtt catggatggc tcatggtcag atgtttgtcg
    14881 gggaaaacgg acaatttccg gcagtccgac caatagggtt tgacgagggc attccccgtg
    14941 ctgttaaagt cttgaataag gatgcggttt gtgtcatgaa tttcgacggt cctgtaaaat
    15001 ccgccctttg ctgtgctgtc ggcgggtttg ccgtgtgcgt caaaatacag gacggtgcgg
    15061 ttgtgaagat gcgcgcaatt tgaaacggcg gggcttgccg gtatgtttcg ggtgcaggcg
    15121 gcaagggttg cccaagggaa aagcagcagg aacaggcggg acattgtgtt tcttgtaagg
    15181 ggtaacaaac agtataatgg ctcattttaa tcctcaggcg gcgggagatg gaagcatttc
    15241 ccttcgggct cggggatttc ggattcggaa gcaacagaca atacgggatt tcggaacaat
    15301 atgaaaactt tgaaatttac caaaatgcac ggtttgggca acgattttat ggtgattgac
    15361 gcggtcagtc aggattttac ccccgaggac gtgccgattg cggcatgggc ggaccgcttc
    15421 cggggcgtgg gcttcgacca gcttttggtg gtcgggtgtt cggaaaccga aggcgtggat
    15481 ttccgttacc gtattttcaa tgccgacggt agcgaggtcg ggcaatgcgg caacggagcg
    15541 cgttgttttg cccgttttgt tgcagacaag ggtttgaccg ataagaaaga aatttgtgtt
    15601 gaaacggcca agggcattat ttttccgaaa ttgtccgata acggtatggt tacggtcaat
    15661 atgggcaaac cgaagtttat gccgtctgaa ataccgtttg tccccgaatc gggcgagggg
    15721 gatgatgcct gtatttacgg ggtgcatctc gaatccggca ttcagcctgt cagctgcgtc
    15781 aatatgggca atcctcatgc tgtgattgta gtcgatgacg tggaatgcgc gccggtgcgc
    15841 gaaaccggtt cgcgtatcga accgcacagg cagtttcccg aacgcgtcaa cgtcggcttt
    15901 atgcaggttg tcggacggac ggcaatccgt ttgcgcgtgt tcgagcgcgg cgtgggcgaa
    15961 acccaagcgt gcggtacggg cgcgtgtgcg gctgtggtgg cgggtatccg tctggggctg
    16021 ttggatgaag ggaaaacggt agaggtggtt ttgccgggcg ggactttata tatcgaatgg
    16081 gcctgcggcg gcgatgtgat gatgaccggc cctgcggaaa ccgtgtttga aggcgagttg
    16141 gcgtattcat gattttgctg catttggatt ttttgtctgc cttactgtat gcggcggttt
    16201 ttctgtttct gatattccgc gcaggaatgt tgcaatggtt ttgggcgagt attgcgttgt
    16261 ggctcggcat ctcggtttta ggggtaaagc tgatgccggg gatgtgggga atgacccgcg
    16321 ccgcgccttt gttcatcccc catttttacc tgactttggg cagcatattt tttttcatcg
    16381 ggtattggaa ccggaaaaca gatggaaacg gatggcaggc agaccccgaa catccgctgc
    16441 tcgggctttt tgccgtcagt aatgtatcga tgacgcttgc ttttgtcgga atatgtgcgt
    16501 tggtgcatta ttgcttttcg ggaacggttc aagtgtttgt gtttgcggca ttgctcaaac
    16561 tttatgcgct gaagccggtt tattggttcg tgttgcagtt tgtattgatg gcggttgcct
    16621 atgtccaccg ctgcggtata gaccggcagc cgccgtcaac gttcggcggt tcgcagctgc
    16681 gactcggcgt gttggcggcg atgttgatgc aggttgcggt aacggcgatg ctgcttgccg
    16741 aaatcggcag atgattgtga tgccgtctgt atcggaacaa aaagttataa agaaataaat
    16801 tttgggtatt ggttttttag gcggcatagg tttaggataa agctatatcc gaaatttgtt
    16861 tatggttcgg cgcaaatccc ctgcaatcgg acaggatacc gatggggatt gcgccttact
    16921 gtcgaaacct tattattcag gagcagaaga tgaaaattgc aaacagcatc accgaattga
    16981 tcggcaacac gcctttggtc aaactgaacc gtttgaccaa aggtttgaag gcagaggttg
    17041 ccgtgaaact ggaatttttt aatccgggca gcagcgtcaa agaccgcatt gccgaagcaa
    17101 tgatcgaggc cgccgaaaaa gcgggaaaaa tcaacaaaaa caccgtcatt gtcgaagcaa
    17161 ccagcggcaa tacgggtatc ggtttggcaa tggtatgtgc cgcacgcggc tacaaactgg
    17221 cgattaccat gccggaaagc atgagcaaag agcgcaaaat gctgttgcgc acgtttggcg
    17281 cggaactgat tctaaccccc gccgccgaag gtatggcggg cgcgattgcc aaagcgcaat
    17341 ccttggtgga cgctcatcca gacacttatt ttatgccgcg ccagttcgac aatgaggcaa
    17401 atcccgaagt ccaccgcaaa acaaccgccg aggaaatttg gaacgatacc gacggtaaag
    17461 tcgatgtctt cgttgccggc gtcggcacgg gcggtacgat taccggcgtg ggcgaagtat
    17521 tgaaaaaata caaacccgaa gttaaagtgg ttgccgtcga gcctgaggct tcccccgtat
    17581 tgagcggcgg cgaaaaaggc ccgcacccga ttcaaggtat cggcgcaggt tttattccga
    17641 ccgttttgaa taccgaaatc tacgacagca ttgccaaagt gccgaacgaa gcggcttttg
    17701 aaaccgcccg tgcaatggcg gaaaaagaag gcattttggc gggcatttct tccggtgcgg
    17761 cggtttggag cgcgttgcag cttgccaaac agcctgaaaa cgaaggcaag ctgatagtcg
    17821 tgctgctgcc ttcttatggc gaacgctatc tctctacgcc actttttgca gatttggcat
    17881 aatgctttaa tcggattgtc gaaacattca gacgcatttt tcggtatcgg tgtaacgccg
    17941 tgccggaaaa tgcgtttttg catatatacc gaaaacgccg gttgtgtttt aatcaggtgt
    18001 tggcgccgcc gcattgcttg agggaaatat tttttattcg ggttttatcc ggcaggacgg
    18061 attttgcccc aacggaaaat agccggcctg cccgtaaagt cagccgtttg tccgggcgca
    18121 gccggggctt tgggcttcag acggcatatt ttcggaatgg cggcactttt gccggcggcg
    18181 cggcagccat atggggaagg gaggggatat tgtggtcggt aacggaaaaa aaatatgccg
    18241 caccattgct ggtgctgggt tgcgcggtgt tcggattggg cagcctgatt gtcagatccg
    18301 tccccgtcgg cccgtatgca atcgcatttt ggcggttgct gatttcggta ttcgtatttt
    18361 ggtttttggc acggtttttc ggacaaaaat tcccaaaaaa cagaaaaacc gtccgatatg
    18421 ccctgacggc gggcgtgttt ctcgctttcg atttggcact ttggcacgaa agcatacacg
    18481 cggtcgggcc gggtatttcc accctgctca acagcctgca aatctttttc ttgtcggcaa
    18541 tcggtgtttt ctttttcggc gagcgtttgg gcaggctgca ggcggcaagc ttgatgtcgg
    18601 cggttgtggg cgtggcgatg attgccggtg cggaattcgg ctacaacggt aatgcggttt
    18661 ggggattcgc cagcggtttg gtgtccgggc tgatgcttgc cctatctatg gtgttcgtcc
    18721 gcaagactca tgaagtcgaa caggtggcgc ttttcccttc aatgatgatt ttgagtttgg
    18781 gcggcgcggc atcgccgatt gttccggcat tgctgatgga cggcggcgca ctttatccga
    18841 ccacttggaa agattcgggt ttggtgcttg tgtacggggc gtggtgatgc agtgttttgc
    18901 gtgggcgatg gttgcctatg cgattccgct gctttccctg tcgctgacgg ggctgttgct
    18961 tttgtccgaa ccggtcgccg ccctgttcat cgattatttc ggattgggca aaacgattga
    19021 aggcgtgcag tgggcagggg tggtgctgac gctttcggcg atttacctcg gttcgctgaa
    19081 acagccgtcc gagctttgac tccggtcggc atcagatttt ctgccgtggc aaacctgcgt
    19141 acgattggac tggcaggcag tcaaaaccgg caagaccaaa catgccgaca acggcgcgga
    19201 gcggaaaatg ccgtctgaag ccctttcaga cggtatcggg gcgggcagaa tcaaaaaagc
    19261 cgcctccgca ggaatggcaa tgcaactaaa ataatgtccg tatttccata tgccgcccac
    19321 accgtcagtc ggacgaaatg ccctttattc aatgggatgg gtttccacac aaacggaaat
    19381 aaccggcttt tcacttgccg acggataaaa tcaaccgatg atttaaacac aggcaaatca
    19441 tacgccgccc gaacgcatca tttttccagg caatccatat gtgccgccca tatcgataat
    19501 gccggcaagg cggtgcatat agtggattaa attcaaatca ggccaaggcg acgaagccgc
    19561 ggacagtaca aatagtacgg caaggcgagg ccaggctgta ctggtttaga tttaatccac
    19621 tatattatcc ccgaggctgc cggtagggaa acaaggcgtt tgatgataaa aatgccgtct
    19681 gaaacagggt tcggacggca tttttgatgt tccggcgtaa ttttccggct taacggattg
    19741 ccgtgccggc cctgccgaaa tcgccgaagt tcatcaaaat gaacattgcc ttgccgacaa
    19801 ccagcttgtc atccacaaag ccccagtagc gcgaatcgcc actgttgtcg cggttgtcgc
    19861 ccatagcgaa atagcgtcct tcgggaactt tgcacacgaa accgctgccg tcgtcggcat
    19921 attggcaatg gttcagaccg ctttgatcta tggaatatcc gttttcagac ataatatcgg
    19981 aggtatattt gcccaatacg ggcagggaaa cggcaggctg tccttctttt ttcagaatat
    20041 tgaaggattt gccgtccaga ccgctgcgga acatatccgt gttgtggatt tcggatgggt
    20101 cggtatcgtc aggataacgg tatgtgccgt caggaatgtc ggaagccggt tttccgttta
    20161 ccgtcaaaac tttatctcga tattcgacaa tgtcgcccgg gatgccgaca atacgcttga
    20221 tgtaggtcat ctccggctgc aaaggatagt taaaaacaac gacatcgccc cgttcgattt
    20281 tgcccgtggg gatgaataca ttgtttaaaa cgggtacgcg caggccgtag gaaaatttgc
    20341 cgaccaaaat gaaatcgccc ttgattaatc ccgggcgcat cgagctggac gggatttgga
    20401 acggttcggc aataaacgac cggatgagga acaataccaa aacggtaggg aagaaactgc
    20461 cgaaataatc gccgaagtgg ctgctttccg agatttcggg atgagtcttc aggcggtatt
    20521 tatatacccc ccaagccgta ccgcacaata caacgaaaat caggaaaacg gcggtaaagc
    20581 tcataaacag ggacaaagcg gcaaacacac cgaccgccgt caggatatag gcgtattcga
    20641 ggccggaact ccattcaccg ttttcctgcc gtttcttgtc gcttttgaaa taaaggatga
    20701 tgccggcaag cagcgcggca gccgcgcccg acattagcat tgtgttcatt gttgttcctt
    20761 aacggttaaa aacccgcccg gccgtgcaac cgttttaagg cggcaaattg caaaatttgt
    20821 ttgcgggcgc gtgccgctga aatcagggcg gtttgagagg tgttcccgac gcgcccgccc
    20881 tatgtgccga aatattattt gtcgctcacc tgcaaaatcg ccaagaacgc gctttgcgga
    20941 atttccacgt tgcccacttg tttcatacgg cgtttgcctg ctttttgttt ttcaagcagt
    21001 tttttcttac gcgtaatatc gccgccgtaa catttcgcca aaacgttttt acgcagggct
    21061 ttaacgtttt ctcgggcgat aatccggctg ccgatggcgg cttggacggc gatgtcgaac
    21121 atttggcgcg gaatcagttc gcgcattttc gatgccagct cgcggccttt gtgaaccgcg
    21181 ctttgacggt gtacaatcag gcttaaggca tcgacttttt cgccgttaac cataatgtcg
    21241 agcttaatca aatcagacgg ttggaattct ttgaaatggt agtccaacga agcataaccg
    21301 cgcgaagtgg atttgagttt gtcgaaaaag tccattacga cttcgttcat cggcaagtcg
    21361 taagtcagca tcacttggcg gcccatatac tgcatattga cctgcacgcc gcgcttttgg
    21421 ttacacaaag tcatgacgtt gccgacgtat tcctgcggca caaggatggt cgcggtaata
    21481 atcggctcga gaatggtttc gatgctgccg atgtcgggca gtttggacgg gttttcaact
    21541 tcgatttttt cgccgctttt caacacgact tcgtaaacca ccgtcggcgc ggtggtaatc
    21601 aaatccatgt cgaactcgcg ttccaagcgt tcctgcacga tttctaaatg cagcaggcct
    21661 aagaagccgc agcggaagcc gaagcccaat gcttgagaaa cttcaggctc gaatttcaac
    21721 gaagcatcgt tcagctgcaa tttttccaaa gcgtcgcgca aggcttcgta atcgtggctt
    21781 tccacaggat agagacccgc aaatacttgg ctttgtactt cttggaaacc gggcagcggc
    21841 tcagtggcag ggttggcaac caaagtaacc gtatcgccga ctttcgcctg tcccaattct
    21901 ttcacgccgg taatcaaaaa acccacttcg ccggctttta gttcttgttt ttgaaccgat
    21961 ttcggtgtga atacgcccag ctgctcgacc tgcgtttccg ccttggtgct cataaagcgc
    22021 actttgtctt tcagtttgat ggtgccgttt ttcactcgaa tcagcatgac cacgccgacg
    22081 taattgtcaa accacgaatc gacgataacg gcttgcagcg gcgcgttttc gtcgccggtc
    22141 ggcgcaggaa ttttggcaac gatttcttcc aaaacgtctt ccacgccgat gccgcttttg
    22201 gctgaacact gcaccgcgcc gacggcatcg atgccgatga tgtcttcgat ttcctgttcc
    22261 acacgctcgg ggtcggcggc gggcaggtcg attttgttca aaacgggcac gacttccacg
    22321 cccaaatcaa tcgcggtata gcagttcgcc acggtttgcg cttccacgcc ctgcgacgcg
    22381 tcaacgacca aaagcgcgcc ttcgcaagcc gacagcgaac gggaaacttc gtaagagaag
    22441 tcgacgtgtc ccggcgtgtc aatcaaatta agctgataca cctgcccgtc gcgtgctttg
    22501 tagttgagtg cggcggtttg cgctttgatg gtgatgccgc gctctttttc gatgtccatg
    22561 gaatcgagca cctgcgtact catttcgcgc aaatccaaac cgccgcaata ttggatgaag
    22621 cggtcggcaa gcgtcgattt gccgtggtcg atgtgggcga tgatggagaa atttcggata
    22681 tttttcattg agttgttttt aatgctgaac ggtaggtttt agaaatgccg tctgaacaaa
    22741 cagcggtacg tccgaatatc gggcgcaacg tggaaatagc ccgatattct aacggaaaac
    22801 cgctgttttg gcatgatttg ataaaggttt tataaagatt tgacgatttc tgccaccatt
    22861 tttgcagaac ttgccgccgc cgttttcaaa aactcttcaa agctgatgtc tgccttttcg
    22921 tccgccgaat cggaaacagc gcggatgatg acgaacggcg tttccaactg atgacaggtt
    22981 tgggcaatcg ccgccgcttc catttccact gctttgactt cggggaagtg cttgcggatt
    23041 tccgccacgc cttcgctgct gtggacaaag cggtcgccgc tgacaatcag gccttgttct
    23101 acctccgcgc cttcaaacgt ccgcgccgcc cgttttgccg tttcaatcaa tatgccgtct
    23161 gaagcgaacc ttgccggcag ttgcggcact tgcccccggg catagccgaa tgcggttaca
    23221 tcgacatcgt ggtgctcggt ttccgtgcca atcaccacgt cgccgacttt caaacccttg
    23281 cccaaaccgc ctgcgctgcc ggtgttgatg acgcagtccg gtgcgaattg acggataagc
    23341 caagccgttg caaccgccgc gttgaccttg ccgatgccgc tcaatgcaag caccatgcgt
    23401 tttcccgcca attcgccttc ataggcggaa aatctgccga aagagacggc tttgacattt
    23461 tccatcatct cgcgcaaaag ctcgatttcc tgttccatcg cgccgataac ggctactgtt
    23521 ttcaaagaca tattgctaac ctgctgtgaa tttcggatag aacgccgaat tatacacgct
    23581 aacacggcag gattgagtgg aggcggtttg tccgtgccgt ctgaaacggt ttcagacagc
    23641 acggcggatt tttggtagaa tgggaaggta cagattgttt gaagattggg ggacgaggat
    23701 gtttaccgat gaaaatatga ctgcaaagga agaactgttc gcatggctgc gccatatgaa
    23761 caaaaacaaa ggttccgacc tgtttgtgac gacccatttc ccgcccgcta tgaagctgga
    23821 cggcaaaatc acccgcatca cggacgaacc gctgacggcg gaaaaatgta tggaaatcgc
    23881 cttttcgatt atgagtgcga agcaggcgga agaattttca tcgaccaacg agtgcaactt
    23941 cgccatcagc ctgccggaca ccagccgctt ccgcgtcaat gcgatgatac agcgcggtgc
    24001 gacggcgttg gtattccgcg cgattaccag caagattccc aagtttgaaa gcctgaacct
    24061 gccgccggcc ttgaaggatg ttgcgctgaa aaaacgcggg ctggttattt ttgtcggcgg
    24121 caccggctcg ggcaaatcga cttcgctcgc ctcgcttatc gactaccgca atgaaaattc
    24181 gttcggacac atcatcacca tcgaagatcc gatcgagttt gtccacgaac acaaaaactg
    24241 catcattacc cagcgcgagg tcggcgtgga cacggaaaac tggatggcgg cgttgaaaaa
    24301 tacgctgcgt caggcgccgg atgtgatcct tatcggcgaa atccgcgacc gtgaaacaat
    24361 ggactacgcc atcgcctttg ccgaaacggg gcatttgtgt atggcgacgc tgcacgccaa
    24421 cagcaccaat caggcgctcg accgcatcat caacttcttc cccgaggagc ggcgcgaaca
    24481 attgctgacg gatttgtcgc tcaaccttca ggcgtttatt tcgcaacgcc tcgttccgcg
    24541 agacggcggc aagggcaggg tggcggcagt cgaggtgctg ctcaattcgc ccctgatttc
    24601 ggagttgatt cacaacggca acatccatga aatcaaagaa gtgatgaaaa aatccactac
    24661 cctgggtatg cagaccttcg accaacacct ttaccaattg tatgaaaaag gcgagatttc
    24721 cttgcaggat gccttgaaaa atgccgattc cgcacatgat ttgcgtttgg cggtacagtt
    24781 gcgcagccgc agggcacaaa gttccgaccc cgatttggaa ctgctctgat ggcggtatgg
    24841 atttccggac gggcagtttg acatgattta tccgtggcat caggaacaat ggcggcagat
    24901 tgccgagcat tggacgagcc gtcctaatgc gtggctgttt gttggcaaaa aggggacggg
    24961 gaagactgcg tttgcccgct ttgcggcgaa ggcgctgttg tgcgaaaccc ctgcaccggg
    25021 ctgcaaaccc tgtggcgaat gtatgtcctg ccatctgttt ggacggggaa gccatcccga
    25081 tttttacgaa atcacgcctc tggcggacga acccgaaaac ggacgcaaac tgttgcggat
    25141 caaaatcgat gccgtcaggg aaatcatcga taatgtgtac ctgacttcgg tacggggcgg
    25201 tttgcgcgtg atcctgattc atcctgcgga aagtatgaat gtccaagccg ccaacagttt
    25261 gttgaaagtg ttggaagagc cgccgccaca agtggtcttt ttgctggtaa gccacgcggc
    25321 ggacaaggtt ttaccgacca ttaaaagccg ctgccggaag aaggttttgc ccgctccttc
    25381 gcatggggaa gcattggcgt atctgcgcga caggggtgtg gcggagcctg aggaacgtct
    25441 ggctttccat tcgggtgcgc cgctgtttca ggaggaaggc gagcttcggg aattgagggc
    25501 aaagctgctg gagatattgg cagagccgag attgttgaag attttggatt acgccgcgct
    25561 tttcgataag gaaaaacttc cgctcgccgt atttgtcggg tggatgcaga aatggctggt
    25621 cgatttaggc ttgtgcctgc aacacatgaa acccgtctat tatcccgctt atgaagacag
    25681 gctgcttcag acggcatcgg gtttccgtcc gcgcaatgta tttgcggcgg aggatatgct
    25741 caaacagctt gccccttacg ggtttcatac tttaaatgtc aaaatgcaga tcgagcatct
    25801 gctcatcaac tatttggaat tgaagaaaga gaacaggtga attatgtcag acggacaaaa
    25861 tattccggca aaaatgatgt cgttgcagct gaaagacatg aatctgctgt acagctccta
    25921 tatgccgttt ttggaacacg gcggtctgtt tgtgcagacc gacgacgtat tttccatcgg
    25981 ggacgatatt ctgcttgccg tagaaatcct caacttcccc aaactgttcc tgccgaccaa
    26041 ggtcgcctgg attaatcctg cgagtacttc ctccaaaccc aaaggggtgg ggctggcatt
    26101 cacaaaacac gaaaactgcc tgaaagtcaa agaccagatc gaagtcgaac tgggcagcac
    26161 aatcagcggc agcagaccta cgtttaccat gtaacgctat gcatatcatc gattcgcact
    26221 gccacctcaa ttttgaaggt ttgaaagaac gcctgcccga agttttgtcc aacatggaag
    26281 caaacggcgt ggggcaggca ctcgccatca gcgtcagtag ggaaagcttc tccgaagtct
    26341 ttgccgtcgc cgaagcgcac gaacacatct attgcaccat aggcgtacat cccgacagca
    26401 aggaagccga agaattttcc attgcggaaa tggtcgaagc cgccgcccat ccgaaagtgg
    26461 tcggcatcgg cgagacgggt ttggattatt actggtgcaa aggcgatttg tcttggcagc
    26521 acaaacgctt tgcagaccac atcgaagcag ccaatcaaac cggactgccc gttatcgtcc
    26581 atacgcgcga tgcggcggcg gacaccttgg cgattttaaa agaatgtaaa accaattcgg
    26641 gtgtgattca ctgtttttcc gaggatgtcg gttttgcccg ggctgccatg gatttggggc
    26701 tttatatttc tttctcggga atcgttacct ttaaaaacgc ccccttggtt caggaggcgg
    26761 cgaaatacgt tccggacgac cgcattttag tggaaaccga tgcgccgttc cttgcccccg
    26821 ttcccaaacg aggcaggcag aacgaaccgg cttttgtgcg tcatactgcc gaacatattg
    26881 ccaaattgcg gaatcaaaca ttggaacagg ttgcggcata tacgacggaa aacttttacc
    26941 ggctgtttaa aaaagtaccc gatatgcggg tcgtctgacc ctgtgccgac gataaggaaa
    27001 accatgaagg caattcatcc gtatgcatgt ccgcgctgct gccggctgcc tgccaacacg
    27061 tttcggacag gcatggcaaa ttccgcttcc aaattctgca ttgccaaagg cggcagacgg
    27121 gaagtaaaaa aagacgaaag cggcggcgga tatgccctgt gccatttgcc ggacagcagg
    27181 attgtcgagg agtgggaata ttgccgttcg caacattgat actgcgcgat atacggcaaa
    27241 tattgtggga agtttccgct tttgcgtata atgcgcccta cctgacaaat tttgtcaact
    27301 ttatcaaaag gaataagcga tggcttccat ccacgaccaa attaaagaag tagtaacgac
    27361 acaccgcgtc gtattgttta tgaaaggtac gaagcagttt ccgcaatgcg gtttctcttc
    27421 ccgcgccgtg caaatcctga acgcggcagg ctgcaccgat tacgttgctg tcaacgtatt
    27481 ggaaaatccc gaagtacgcc aaggcattaa ggaatacagc gactggccga ccatccccca
    27541 actttatgtg aacggcgagt ttgtcggcgg ttcggacatc ctgatggaaa tgtatgaggc
    27601 aggcgagctg caagagctgc tgaaagcctg atggattcgg caatgccgtc tgaacgtgtt
    27661 tcagacggca ttttcttttc cggcaaatta aaaaaaagta taatgacgcg tctcaaaatc
    27721 acactggaac accgcgatga acgttaacgt tatcaaccat ccgctcgtcc gccacaaact
    27781 gaccctgatg agggaggcgg attgcagcac ctacaaattc cgtacgctga ccaccgagtt
    27841 ggcgcgcctg atggcatacg aggcaagccg tgattttgaa attgaaaaat accttatcga
    27901 cggatggtgc ggacagattg aaggcgaccg catcaaaggc aaaaccctga ccgtcgtccc
    27961 catcctgcgc gccggtttgg gtatgctcga cggcgtgctc gacctgattc cgactgccaa
    28021 aatcagcgtg gtcggtttgc agcgcgacga ggaaacgctg aagcctattt cctattttga
    28081 gaaatttgtg gacagtatgg acgagcgtcc ggctttgatt atcgatccta tgctggcgac
    28141 aggcggttcg atggtggcca ccatcgacct gttgaaagaa aaaggttgcc gcaacatcaa
    28201 agccttagtc ttggttgccg cccctgaggg cgtgaaggct gttaatgatg cgcatcccga
    28261 tgttacgatt tataccgccg cgctggatag ccgtttgaac gaaaacggct atattattcc
    28321 cggtctgggc gatgcgggcg acaagatttt cggcacgcgc taactgactg attttcggat
    28381 ttgatatgaa tttccaagac tatctcgcca catttccttc aatcgaccat ctgggcggtt
    28441 tggatgttca ggatgccgaa ggcaaaacgg ttcaccacat tcccgccgtt cagggcaagc
    28501 tcggttcgct caagctgtac aatgccttgg cggaacgttt tgatggaaaa ttgggtaaag
    28561 aagcggcaga acagggtttg atatggtttg ccgaacatgt tgccgacgcg cgcgcccatc
    28621 cggggaagca tccgaacatc gatttgctgg aaaatgtcgt gcaaagcggc gaaaccctcc
    28681 tgctcaaacc gcttgccgcg caataatttt cgaccatgcc gtctgaaatt cgtttcagac
    28741 ggcattttgt cggaaagaag accgtaaaac gggcattttc ttttctattt ggctgtgttt
    28801 taacaatccg ttgatttttg cactaatttt tgataaaatc ccttataaca aattgaacta
    28861 taagggattt tataatgtcg ggcgtaacaa aagaattaga cattttaaaa caactttttg
    28921 aaaatttgtc cgatacggat aaacaggctt ttttgacttc tgtcagctca aaagaacagg
    28981 ttaaaaaagt cattgaacca agaaaagtta cgaagtgccc acattgccaa tctacacatt
    29041 tcgtcaaaaa tggcaaagat tgtggcaacc aacgtttttt gtgtcgtgat tgtaaaaagt
    29101 cttttgttga gcagacaggc actattctct acaacacgca aaaagacatt gaagtttggg
    29161 aaaaatacat tcattgtatg attgaaaaat acccacttcg caaatgtgct gaaatttgca
    29221 aaatcaatct tgctactgcg tttacttggc ggcacaaaat tttggacgca cttcaaaaca
    29281 tgatgaatga agttgagtta gacggcattg tgcaagctga tgaaacttat tcgacgattt
    29341 cttacaaagg gcaccacaaa aattttaatc tgccacgtcc tgctcacaaa cgaggaacga
    29401 gagcaacgaa acgtggcatt tccaaagaac aagtttgtgt tccttgtggt atcaatttag
    29461 acggtaaatc tgttgctaga attagcaatt tgggtaagcc atctctaaaa aatatcaact
    29521 gattggcatt gaaaacaacc caaaaatgtt tgctcttgcc gccagtaata tgattttacg
    29581 cggagacggt aaagccaatt tacaccaaag cagttgtttc atgactgatt ttcaggattt
    29641 aatcaaaaat cccaaacctg aaacaggctt aaaacgccca aatgttggct ttctcaatcc
    29701 accttacgcc caatccaaaa gcgatgccga attgcatgaa ctttattttg tcaaagaaat
    29761 gttggatatg ttggcagaag gtggcacagg cattgccatt attccagtca gttgcgtgat
    29821 tgcaccaagc aaagccaaaa gcgaaattgt gaaatatcat cgcttaaaag ccgtgatgtc
    29881 tatgccgagc gaactgtttt acccagttgg cacggtaacg tgcattgtcg tatttgaagc
    29941 ccataaaccg cattttcaga cagtcgtgat tgacccggac acacaagaag aaatcagcac
    30001 gaaaaaagcc tgtcgcaaaa cgtggtttgg ctactggcgt gatgacggtt ttgaaaaaac
    30061 caaacacttg ggacgcattg atttatacga ccgctggcag ggcattaaag cgcgctggtt
    30121 ggaacattat ttaaacaacg aagttcacac aggagaatcg gtaacagcat ttgtaactga
    30181 taacgatgaa tgggttgccg aagcctattt ggaaactgat tattccaaaa ttacccgagc
    30241 agattttgag caagtcgtgc gtgaatttgc tttatttcaa ctactgggag cggaagtagg
    30301 gccgactgaa aatttggata atgaaagcta tgaagacgat gacaataacg acttcggaga
    30361 cgatgaataa tggttgaatt gcaagagatt tttgatgtga gttacggttc aaaattagat
    30421 ttgaataaaa tgagcagctt caatccaaca atcaactttg taggcaggtc aggcaaaaat
    30481 aatggtgtaa cagcatctgt ggatttattg aaaaatacaa aaccttatcc agcaggatta
    30541 ttaactgttg cattaggtgg ttctgtttta tctacttttt tacaaaacaa accattttat
    30601 acagctcaaa atgttgctgt tttaaatccc aaaacagaaa tgacagaaca acaaaaactc
    30661 ttttattgtg ctgctatttt tgccaatgcc taccgttttt ctgcttgcgg tagggaagct
    30721 aaccgcacgt tacgacaact gtttgtgcca tctttggatg agatcccaag ctgggttgaa
    30781 agcgtgaatt taaatccatc tgcgggtgta accgaaccga aattaaaaga accgcttgat
    30841 ttgcctgtgg ttcgacaatc aaaacgactt gatgaaattt ttactattca aaacggcatt
    30901 gccgcaacca aattaaaaga atttgaacaa cggcaaaaag acacggttgt ctatatccgt
    30961 cctgccagta cgcaagcaag aacgctacga agttatatcg ctcgtgatag cgttgatgaa
    31021 aaacatattt ttccatgcca tacgcttttc acttctacca atggcgaagg tagccatact
    31081 tatagctatg tttcaacttg tgaatttgta gcaaatagtg atgtcgccgt attaacaccc
    31141 atacagtccg atatgccaat agaagtaaaa ctgtattacg ctaaatgtat tacagcaaac
    31201 cgctatctgt tttcttatgg acgtaaaccc aaaggcgaaa aattaaaaag cattatgtta
    31261 ccgtattttg accaacaaga agattttgat tatatctgcc gtttcattca caccttgcta
    31321 tttagtaaca atttgtagat taccaccctt ataaacaaaa caacccgccg taatagatga
    31381 aatagagaac tgttttttag tgtgaattca tcttaagcaa tcaacggatt gttaaaacac
    31441 agccttctat ttcaggatac gggcaatgat gtttcaacac acaggacgac acataaagcg
    31501 ccgccctatg tgttgcccta atttgaaagg ggttacaccc ctttcaaata aaatctgatg
    31561 ctgctgccac gaaggacgga tgtccgaatg gcggggtttc aaccattaag gaaattatga
    31621 tgaaaaaaat gttcctttct gccgcattgc ttctgtcggc tgccgcccaa accgtgtggg
    31681 cggatacggt attttcctgt aaaacggaca acaacaaata catagaagtt caaaaaatca
    31741 accgcaatct ttacgaatat tcgttcggca gtgcggcaaa aaaagaaatt gccatacgca
    31801 acagcaaagc tgacctgttg gggcgttccg acaggtggca aggcatgggc agcggacgcc
    31861 gggcaacgat gaaattccaa aacggcgaat ttatgtacac cgtatggaca ggcttcgatt
    31921 ccgtgactca tacggaaagc agcggtgtcg ttgtggagcg taggggcaag gaagtcgcac
    31981 gggtaggctg tacgccgaaa accgcgcagg cgaatttcaa cgatgacgat ttttcccggt
    32041 aatcggggcg gataaggcga tggaaacagc gaagcccgtc atgctgattg tccgcccgtc
    32101 gggcagggcg aaggacgatg tggaagtctg ccgtcgtgcc ggatggcagg cggaagtgtt
    32161 gagtccgatt gaaatcgaaa ccgacgaagc aagcctgaaa cggctgccgg aaatgtatgc
    32221 ccgtgcggat gccgtgtttt gggtcagtcc ggccgccgtt gaaaccgccg tcccgtacct
    32281 taacctttca gacggcataa aggcgcacat tgccgtaggg caaggcagcc gccgtgcctt
    32341 ggcgcgttat gcgggcgcgg gcgtgtttgc acctgaagac ggcaacgaca gcgaggcggt
    32401 tttgcgcctg ccggtttgga acagtctgcc cgaaggtgcg cgcgtattgt ctgtgcgcgg
    32461 acacggcggg cgggattttc tgatgaatgc cttgcaggag aaaggttttc ggacggaggt
    32521 ggcagaagtc tatttcagac ggcataaacc tttgaacttt caaaatttcc aaaccgaaaa
    32581 tattgccgcc gcctatatta cgtccacaga acttgtgcag tcgctgtttg cacagcttcc
    32641 gccgcaattt tcccgattct tcaaatcctt gctatacttt acccatcatc cgcgcattgc
    32701 ggaggcattg aagcgcgaag gcgtgtgttc ggtcgaaacc gtccctacgc tggaagccgc
    32761 gctttcctat tcttccattt ccgtttcaga cggcatggtc tttcccggaa cctcaaacca
    32821 ataaggagca aaacggtggg cgaacctgaa aacaaatcat ccgaacccgt acgcgagata
    32881 caggcatcaa aagaaatgcc gtctgaaacc tcttccccac gcaaagaaaa cgaaacagaa
    32941 gtacacattc ccgccgctcc ttttatcgtc aaacagtccg gcagcaacgc tttggcggcc
    33001 tgcgccttgg tattggcggc attgggtttg ggtgcaagtg gttttttgtt tgtccaagga
    33061 cagaatgtct tgaaaaacca agagctggca ttcaaccaga aaatcgacaa agccgccttg
    33121 ggcgagtcgg aaaacgccgc cctgctcaaa gacaacctca accgccaaag tgccatacag
    33181 tccgaactcg accgtttgga cagcggtgtc aaagccaacg gcgaacaaat cctgatgacg
    33241 caaaaagcct atcgcgagct gaccaaaggg cgtgccgatt ggctggtgga cgaaaccgag
    33301 accatactca atctggcggc gcaacagctg gtgttgaccg gcaatatcca aacggcagtc
    33361 ggcgtgttgg agcatatcga cagccgcctg tcccgtttcg atcaggcaga gcttctgccg
    33421 atcaaacagg cggtcagcag cgatttggcg gaactgaaaa accgtcccta tgtcgatatt
    33481 tccggcacgg cattgcgcct cgaccggttg gaaaccgccg tatccggact gccgctgatt
    33541 ttggacggcg tattgaaacc gggcgtacag gcccgcaacg aagccgtttc cgcttcatgg
    33601 tggcagaacg tatgggaaaa atccctcggc acattgaagg ggctggtcga aatccgccgt
    33661 ttggaaaaca acgatgccat gctgatttct cccgaacagg catattttgt gcgtgaaaac
    33721 ctgcgtctgc gcctgttgga tgcgcgcacc gcattgatgc agcgcaacgg cgaagtctat
    33781 cagggcgatt tgaacaatgc cgaagccgcc gtcagacagt atttcgatgc caagtcgccc
    33841 gccacgcagt cgtggctgaa agaactggcg gaattgaaaa cactggatgt acgcatgacg
    33901 gcggatgacg gcttgaagac cagtttaaat gccgtccgcg cctatcgcga cggtacgcgc
    33961 atgacggcgg cggaaaatca agaagcggaa caggcggctt ccgaaccggc aaacgaaaac
    34021 acagcttccg aaccggctgc cgcatcggat gtgaaggcca tagaagcacc gtccctgcct
    34081 tcggaacgca aaccggaaca gcctgcaaaa aaacagcccg caccggaaaa ggcagggcgt
    34141 tcgccgtccg ctaaaggaga acgcgcatga aaacggtagt ctggattgtt gtcctgtttg
    34201 ccgccgccgt cggactggcg ctggcttcgg gcatttacac cggcgacgtg tatatcgtac
    34261 tcggacagac catgctcaga atcaacctgc acgcctttgt gttaggttcg ctgattgccg
    34321 tcgtggtgtg gtatttcctg tttaaattca tcatcggcgt actcaatatc cccgaaaata
    34381 tgcggcgttc cggttcggcg cggaaaggcc gcaaggccgc gcttgccttg aataaggcgg
    34441 gtttggcgta tttcgaaggg cgttttgaaa aggcggaact cgaagcctct cgagtgttgg
    34501 gcaacaaaga ggccggagac aaccggactt tggcattgat gctgggcgcg cacgcggcag
    34561 gacagatgga aaatatcgag ctgcgcgacc gttatcttgc ggaaatcgcc aaactgccgg
    34621 aaaaacagca gctttcccgc tatcttctgc tggcggaatc ggcgttaaac cggcgcgatt
    34681 acgaagcggc ggaagccaat cttcatgcgg cggcgaagat gaatgccaac cttacgcgcc
    34741 tcgtgcgtct gcaacttcgt tacgccttcg atcggggcga tgcgttgcag gttctggcaa
    34801 aaaccgaaaa actttccaag gcgggcgcgt tgggcaaatc ggaaatggaa cggtatcaaa
    34861 attgggcata ccgccgccag atggcggatg ctgccgatgc cgccgctttg aaaacctgcc
    34921 tgaagcggat tcccgacagc ctcaaaaacg gggaattgag cgtatcggtt gcggaaaagt
    34981 acgaacgttt gggactgtat gccgatgcgg tcaaatgggt caaacagcat tatccgcaca
    35041 accgccgccc cgagcttttg gaagcctttg tcgaaagcgt gcgctttttg ggcgagcgcg
    35101 aacagcagaa agccatcgat tttgccgatt cttggctgaa agaacagccc gataacgcgc
    35161 ttctgctgat gtatctcggc cggctcgcct acggccgcaa actttggggt aaggcaaaag
    35221 gctaccttga agcgagtatt gcactgaagc cgagtattcc ggcgcgtttg gtgttggcaa
    35281 aggtttttga cgaaaccgca cagtcgcaaa aagccgaagc acagcgcaac ttggttttgg
    35341 caagcgttgc cggggaaaac cgcccttccg ccgaaacccg ttgaaacctt cagacggcat
    35401 cgttgaaatg ccgtctgaaa accatttcac ttgacaaaca gactgacaga aaacccgtat
    35461 gactctttta aaaaacgata ccttcctccg cgccctgctc aaacagcccg tcgaatacac
    35521 accgatttgg atgatgcgcc aggcggggcg ttatctgccc gaatacaaag ccacacgcgc
    35581 gaaagcgggc agcttcctcg atttgtgcaa aaacaccgga ttggcgaccg aagtcaccat
    35641 ccagcctttg gaacgcttcg atttggacgc ggcgattttg ttttccgaca tcttgaccgt
    35701 tcccgatgca atgggcttgg ggctgtattt tgccgaaggc gaaggcccga aattcgcaca
    35761 cgccttgcaa cacgaatccg acatcgccaa gctgcacgtt cccgacatgg aaaaactgca
    35821 atacgttttc gacgcggtaa cttccatccg taaagcattg gacggccgcg taccgcttat
    35881 cggcttctcc ggcagcccgt tcacgctcgc ctgttatatg gtcgaaggcg gcggcagcaa
    35941 agaattccgc accatcaaaa ccatgatgta ctcgcgcccc gatttgctgt acaaaatcct
    36001 cgataccaac gcccaagccg ttaccgccta cctcaacgcc caaatcgacg cgggcgcgca
    36061 ggcggtgcag attttcgaca cttggggcgg cgtgttgagc gacgcggcgt ttaaagagtt
    36121 cagcctcaaa tacatccgcc aaatcgttgc cggactcaaa cgtgaaagcg aaggtcgccg
    36181 cgtgcctgtt atcgtgtttg ccaaaggcgg cgggctgtgg ctggaaagca tggtccaaat
    36241 cggcgcagac gcattgggct tggactggac gtgcaacatc ggcgaagcac gccgccgcgt
    36301 cggcaaccaa gtcgccctgc aaggcaactt cgacccgtcc gccctcttcg gtacgcccga
    36361 atccatccgc accgaggtcg cacgtatcct gaccggctac ggacacggca gcggccatgt
    36421 cttcaacctc ggacacggca tcaaccaaca cgccgacccc gaacacgcca aaatcttagt
    36481 cgacaccgta cacgagctgt cgcggcagta tcacggcggg taagccggca ggaaaccgcc
    36541 cgatatgccg tctgaagaat cggaagtttt cagacggcat atgcttcatt ttgcactttg
    36601 gtacaccaag ccattgtgtg gtagcttgat attgggtaac caaaaatctt ttccctgcca
    36661 acctttgtct ttatttccta ctacttgata aagcgtaagg aataaatcat tgggatattt
    36721 ttctcctaat ttacggtcgt cttccgaaag cattgtgccg gttgctcgag aaagtttacg
    36781 tccggttttg atcaaaacat aggttttaaa acttggacgt tgtgctttta atgcttctac
    36841 accagcaata aatttttcct tattccagtc atctggaaca taacgaccca atttatctag
    36901 aaccaagaat aaatcttcta tatctatttg atataaatca gattgaattt catctttcaa
    36961 aatagaaggc aaaattttgt taatctccga taaattatct tcgtttgggt ggagtggaaa
    37021 ataattcaca ccacccacaa tttgattgat actatcgaat ttcaatacat tttttcgagt
    37081 tggattgata tttttcggat aaataacctg aatattgccg cctgaatttt ttgcctgccc
    37141 aataatcaga ttatttgctt gattgagttg tacaaagaag taatagacat caaacggaat
    37201 ataaagccgt aataatgatt tatcgcggtc atatccaaaa atacgcgaat gttgccagaa
    37261 agtatctgca ttcggttttt tggcagtgcg actataataa accgtttgta gtttcggaat
    37321 agtcaagcca cgaccaatca cattaccacc aataatgatg ttgaaacctt tttctaagtc
    37381 aaaatctgtt tctgtctgcg agttaacgac aagagtagag atttgtttat tctctaaaag
    37441 tgctgtcaat ttttcgtata tttcatcaaa gtgatgaata tcgggcttgg tcttttgcaa
    37501 atccaaataa ctttctttaa agctacctgc aaggtcttcc ccattattga ctgcttgcac
    37561 taagtcattt aaaaaggctt ggatcttctt tgaaaaggct tgatgatctt gaattttata
    37621 gcttggatgt agtgcaaaat tacagttagc cttatcacat agcgcgaatt ctgcacaggt
    37681 tattagaaaa ctaagcaatg cctgttttgc tccttcggca atttcaccac tctcgtcttt
    37741 catatcatcc aattcgctat caataaaacg aactatatag cttggtggat cagaaaagac
    37801 aaagttgccg ccgatgtatt tttcgcctgc ttcaaagaag tggataaatt caggttgcca
    37861 atcagattct tcatgttgta ataaaagcga ttgaggcgtg gcagtcaact ggataaacag
    37921 gctttgacaa caggaatttt tgatgtcgtt tagcagttta ttgatagtac tggcatcttt
    37981 agccggctta tcagaattag tatttaggct ggcagcatcg gcttcatcgt ccacaataac
    38041 caaaggatag cctttcaggc tactttgact ggcaaacaaa ttacgccaac gtttcaatac
    38101 acgggcattc tttttaatca cgaccaagat ggggttctct gccttcatta cttccataaa
    38161 acttcggtcg tcagcttcag acaatacgat aaagtttttc agattggctt tggctcgttt
    38221 gactgtttga tcctgtaaat ctacgctgtc agtagtaaga tacaaaaaaa ctttgtggtc
    38281 gccatcatca gccagcgcac ttaatacacc caaaacctgt gccgttttgc cactttgaac
    38341 attgcccagc aataaaacat tttgggcgat tttcggctcg gtttgtgaca atttttccat
    38401 aaaaccatca acggtatttt taacagaatc agctaattcg ggaggggtca attggttcaa
    38461 gtaggttcgc aacatttagt ttttcccctt aaatgagagc agccaaacat caggattatc
    38521 ggttgaacgt aactcaaaat gatcgttacc gtattcgcgt agggtttcat gcgtaatctt
    38581 ctcgttattt tgcaggcagc catgactttc caaacgacct ttaatccatt taccaagtgt
    38641 ctttaaatca ttttctgagc ggaagttttt ggagtaatcc cccgatgtct tgcattgaaa
    38701 ttgccaacca tcatcggtaa taactgtaaa agacttcaga accggatacc cttcttgtga
    38761 agtgatgtct ttggatacaa tcaactctac ttcataccaa ggtcgtggtt tgacaaaccc
    38821 tcgtttatct cgtcgacctt ctccaaaaaa tacattcaaa ttactttttt cttcggtttt
    38881 agctggaatt gagaaatgat attctgacgt ttgggcaaat aattgccgaa tctgttctgg
    38941 tgcaattttc tgaacaccca aacaattttc taaatggcta ttatgttcaa taaatttact
    39001 tggtctttca acttcttgga tatttttgcc tagcttatga atagtacttt gaatatctgc
    39061 ctgcaaaatc tgtgcaggct taccgttcaa atgcaacata gtttcataag tacgttccgt
    39121 gctatcccaa aaacacgtta agttagcaga accaattaat ccattgattt tttgataatt
    39181 tttaaaggag tacattttgc cgtgaaattt cacaaagggg gaaacataga cggctcctct
    39241 tttctcatgt cgcaaaaaat catttagttt acataagctg tcatattgta aatggctaaa
    39301 accttccaaa taatgcattc cgaccaataa atctattttt tgaatatgat cgtttagctc
    39361 caaaatttta tgtaactcaa ccactgcgtc attagataca taacctgtcg ccatcaacac
    39421 ttcatccgct gatttaaata aatccatcca aaccgcatta agagattttt cagtgatttt
    39481 ggcattggcg atattggaaa aaactgtatt cattgttttc cccctcgaat tttgcttagt
    39541 cggtcatgga ataaaatttc tttttcttcg actaatttct catgcaaatc tactttctca
    39601 tagttgtctg aaaaaatcgg taaaatgctt tttgccagtt caaccacgcc ctgaggagga
    39661 acagcattac caatctggcg acgtacttca gttgttgatc cgacaaactc aaaatcatca
    39721 ggaaaacttt gaagccttgc tcgttctcta ttagtaaaag cacgcggttc agggaagtga
    39781 tagccccaag taccaccgcc acctgctgca ataattgttt ttgatggctc gttccgatgc
    39841 atacgacgat aaacgtggct aatcatacct ttcacatata aaggatgatc tttaggaata
    39901 tcggtaaaat ttccaccttc aggaattaat tccaacatac ggcgtgtttt atcgctgatt
    39961 ttcagtaatt cattattact ggcattttgt ggaatattgg atatggcctg acctgctgtt
    40021 acatatggtt ttaagccgtt ttcgccagtt tcattgtgcg tcggttccgg atggcgaaaa
    40081 tcaaatcctg tatccaaacg tactccgaca atcagcacac gttcacgaaa ttgaggtacg
    40141 ccaaattctg caaagttata cagcttcgcc tgaacgtaat aaccgcaatt ttcaaagtcg
    40201 gtaataattt gctggatggc ttttttcttg ttggcagtca ataaaccttt cacattctca
    40261 gcaacaaaaa ctttcggttt ttttgcattt acaaaacgta aaaagctttt ataaagattg
    40321 ccgcgctcac cctctaagcc cggctgtttc caaatcatgg aaaaatcttg acaagggaat
    40381 ccgcctaaaa tgatgtcgca atcgggaata gttggatcat tcggattaat ttgttcaata
    40441 tcaccttcta cgatgacatc gccgatattt ttacggaaac tttcgcaagc ccaatgggag
    40501 aaatcgttcg cccaaacagt ttcacaacca gcttgatgaa agcccaaatc caaaccgcca
    40561 catcctgaaa atagagatag gatttttggt ttgttactag aagaattaag atttgagatt
    40621 tgtttagata gcataggatt tcactcctat gcataaattt gataaaaatc cttgtatgga
    40681 ttttaaaggt gaactatgct ct
//
LOCUS       DAXDXW010000006        40665 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXDXW010000006 DAXDXW010000000
VERSION     DAXDXW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40665
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.006"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            177..557
                     /locus_tag="ACFB64_000259"
     CDS             177..557
                     /locus_tag="ACFB64_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC1208711.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKPV
                     TVVPLSSTEPVPLADCHHKMSENPLPDKPHIQCRAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAVKTAVAKAFKLY"
     gene            complement(758..1600)
                     /locus_tag="ACFB64_000260"
     CDS             complement(758..1600)
                     /locus_tag="ACFB64_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFC1208712.1"
                     /translation="MQNTISVFSFKSQNVRTQILGAEPWFCLGDVAEILQIQNARQLP
                     LKDQGIQKSSVATKKGNQELLFINEPNLYRVIFRSRKAEAVKFQDWIFEEVIPQIRKT
                     GGYQITPKTTADDRTGLRRAVAALVGRKRIGYSSAYSMIHQRFNVEAVEGIPADKLPE
                     AVAYVHALTLHTGLAGEVPDREPLPAPQPALPISGNALADIAAMVYYGTRMIELGKDV
                     SAPLKQLGCKQAVTMWTVWHETRSILKRSVAALEVLRGYADKDASGRIAACLEGIYGK
                     AAAR"
     gene            complement(2099..2275)
                     /locus_tag="ACFB64_000261"
     CDS             complement(2099..2275)
                     /locus_tag="ACFB64_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208713.1"
                     /translation="MNPTKQSKKSYESKRVLKHVSFNTEKEANLLEFSNNLDFSKWVK
                     EKLKHELELEKLKK"
     gene            complement(2603..3094)
                     /gene="dtd"
                     /locus_tag="ACFB64_000262"
     CDS             complement(2603..3094)
                     /gene="dtd"
                     /locus_tag="ACFB64_000262"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFC1208714.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQRLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(3168..3926)
                     /locus_tag="ACFB64_000263"
     CDS             complement(3168..3926)
                     /locus_tag="ACFB64_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC1208715.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQRPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAGMNRGDVTRNPVKLGERYYLFKLGAVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            complement(3996..4862)
                     /locus_tag="ACFB64_000264"
     CDS             complement(3996..4862)
                     /locus_tag="ACFB64_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC1208716.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKDALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAVYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKRTGAPDGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(4911..5189)
                     /locus_tag="ACFB64_000265"
     CDS             complement(4911..5189)
                     /locus_tag="ACFB64_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC1208717.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGLSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(5189..5479)
                     /locus_tag="ACFB64_000266"
     CDS             complement(5189..5479)
                     /locus_tag="ACFB64_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC1208718.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHFKRLETLKSEGRLLTAGPN
                     LLPDNPERVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(5482..6012)
                     /locus_tag="ACFB64_000267"
     CDS             complement(5482..6012)
                     /locus_tag="ACFB64_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC1208719.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAAAAVALVAGVVQAAFLYWK
                     HKRLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPTVLFWCGALFLLGSHLAGKNGLK
                     ASIGREIQLPDAVWGKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTALMLFF
                     FIIQGIYLSTYLKKED"
     gene            6320..8636
                     /gene="tspA"
                     /locus_tag="ACFB64_000268"
                     /pseudo
     CDS             6320..8636
                     /gene="tspA"
                     /locus_tag="ACFB64_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
     gene            complement(8809..9225)
                     /gene="gloA"
                     /locus_tag="ACFB64_000269"
     CDS             complement(8809..9225)
                     /gene="gloA"
                     /locus_tag="ACFB64_000269"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFC1208720.1"
                     /translation="MRLLHTMLRVGNLEKSLDSYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGDAYGHIAVEVDDAYEACERVKRQGGNVVREAGL
                     MKHGTTVIAFVEDPDGCKIEFVQKKSGDDSVAYANT"
     gene            complement(9250..10419)
                     /gene="lapB"
                     /locus_tag="ACFB64_000270"
     CDS             complement(9250..10419)
                     /gene="lapB"
                     /locus_tag="ACFB64_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC1208721.1"
                     /translation="MDNELWIILLPIILLPVFFTMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETAQLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(10430..10744)
                     /locus_tag="ACFB64_000271"
     CDS             complement(10430..10744)
                     /locus_tag="ACFB64_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC1208722.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINMDAVTFSYLPGQSVNLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENSRLRAEVKKSARLSGQKLTAPPIQNAAESAK
                     QP"
     gene            11054..12052
                     /gene="ilvE"
                     /locus_tag="ACFB64_000272"
     CDS             11054..12052
                     /gene="ilvE"
                     /locus_tag="ACFB64_000272"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC1208723.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLTLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWLLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKNQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(12298..13083)
                     /gene="fabI"
                     /locus_tag="ACFB64_000273"
     CDS             complement(12298..13083)
                     /gene="fabI"
                     /locus_tag="ACFB64_000273"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC1208724.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(13238..14059)
                     /gene="dapD"
                     /locus_tag="ACFB64_000274"
     CDS             complement(13238..14059)
                     /gene="dapD"
                     /locus_tag="ACFB64_000274"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC1208725.1"
                     /translation="MSLQNIIETAFENRADITPTTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNAVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGAIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSLPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(14269..15912)
                     /gene="pgi"
                     /locus_tag="ACFB64_000275"
     CDS             complement(14269..15912)
                     /gene="pgi"
                     /locus_tag="ACFB64_000275"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC1208726.1"
                     /translation="MDAFTRAWYALERHYQDTCHILLRDRFAAEPDRFERMHERLDGM
                     LFDYSKNRFGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALKFAHSLDDGLYQGITGKRIADFVHIGIGGSDLGPAM
                     CVQALEPFRRQISVHFVSNADPACLDEVLCRLNPETTMFCVASKSFKTPETLLNAEAV
                     KAWYRGAGFSESETARHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAACKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGAEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPCNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            16422..17654
                     /gene="pilG"
                     /locus_tag="ACFB64_000276"
     CDS             16422..17654
                     /gene="pilG"
                     /locus_tag="ACFB64_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC1208727.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGQVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPPLTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLFAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            17728..18588
                     /locus_tag="ACFB64_000277"
     CDS             17728..18588
                     /locus_tag="ACFB64_000277"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC1208728.1"
                     /translation="MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVR"
     gene            18585..19217
                     /gene="coaE"
                     /locus_tag="ACFB64_000278"
     CDS             18585..19217
                     /gene="coaE"
                     /locus_tag="ACFB64_000278"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC1208729.1"
                     /translation="MTAWVGLTGGIGSGKSAAAQYFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLREKTMLLHAFYSGIFASKPTQGKHNG"
     gene            19210..19419
                     /locus_tag="ACFB64_000279"
     CDS             19210..19419
                     /locus_tag="ACFB64_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC1208730.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDGAYR"
     gene            complement(19505..21181)
                     /gene="pilB"
                     /locus_tag="ACFB64_000280"
     CDS             complement(19505..21181)
                     /gene="pilB"
                     /locus_tag="ACFB64_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC1208731.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVGILDVAYKEGMVDLRRAGILKIMQGITSLEEVTANTND"
     gene            complement(21427..21498)
                     /locus_tag="ACFB64_000281"
     tRNA            complement(21427..21498)
                     /locus_tag="ACFB64_000281"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21463..21465),aa:Arg,seq:cct)
     gene            complement(21519..21965)
                     /locus_tag="ACFB64_000282"
     CDS             complement(21519..21965)
                     /locus_tag="ACFB64_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC1208732.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITVSATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGCG
                     VPLMGRQPVLVTGLLIGTVIGVAFMGGIPVGPLIAADILSFVAGKV"
     gene            complement(21969..22943)
                     /locus_tag="ACFB64_000283"
     CDS             complement(21969..22943)
                     /locus_tag="ACFB64_000283"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC1208733.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIRVIQYKTAKLFEAAAQVGAILGKASPGHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRGYFEKIRDYV
                     VRSDALAYSIGEARKAVDCAVAALDALSDSEVKDAMIQLAKESLVRVS"
     gene            23167..23475
                     /gene="rplU"
                     /locus_tag="ACFB64_000284"
     CDS             23167..23475
                     /gene="rplU"
                     /locus_tag="ACFB64_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC1208734.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAQLDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            23500..23772
                     /gene="rpmA"
                     /locus_tag="ACFB64_000285"
     CDS             23500..23772
                     /gene="rpmA"
                     /locus_tag="ACFB64_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC1208735.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKIDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            24007..25191
                     /gene="ubiM"
                     /locus_tag="ACFB64_000286"
     CDS             24007..25191
                     /gene="ubiM"
                     /locus_tag="ACFB64_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC1208736.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTAVKEVKTSDNEAQVFLENGDILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEKRLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(25380..25535)
                     /gene="rpmG"
                     /locus_tag="ACFB64_000287"
     CDS             complement(25380..25535)
                     /gene="rpmG"
                     /locus_tag="ACFB64_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC1208737.1"
                     /translation="MRDKIKLESGAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(25567..25800)
                     /gene="rpmB"
                     /locus_tag="ACFB64_000288"
     CDS             complement(25567..25800)
                     /gene="rpmB"
                     /locus_tag="ACFB64_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC1208738.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(26050..27573)
                     /gene="farB"
                     /locus_tag="ACFB64_000289"
     CDS             complement(26050..27573)
                     /gene="farB"
                     /locus_tag="ACFB64_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC1208739.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAAGFVIASWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRMLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKHRETATVRTPTDYVGLTLMMVGIGALQMM
                     LDRGKELDWFASGEIITLGITALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSAWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDGQTLGIINNTITQQGFIIGSNEIFLAGSILFIVLIPIVWLAKPPFHSGGGGH"
     gene            complement(27597..28751)
                     /gene="farA"
                     /locus_tag="ACFB64_000290"
     CDS             complement(27597..28751)
                     /gene="farA"
                     /locus_tag="ACFB64_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFC1208740.1"
                     /translation="METHTDETKLQNTQVKRKRRLTALTLLFALSAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RTAVSQAQAAVKAALAEESSARAALGGDVSLREQPEVQTAIGRLKDAWLNLRRTQVRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     regulatory      28992..29036
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            29040..29513
                     /gene="queF"
                     /locus_tag="ACFB64_000291"
     CDS             29040..29513
                     /gene="queF"
                     /locus_tag="ACFB64_000291"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC1208741.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAVHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            29588..30274
                     /locus_tag="ACFB64_000292"
     CDS             29588..30274
                     /locus_tag="ACFB64_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC1208742.1"
                     /translation="MYALTAAQQQKALFRLVLFHILIIAASNYLVQFPFRIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     QFNTFVGRIALASFAAYALGQILDIFVFDKLRRLKAWWIAPAASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCTLFFLPAYGVILNLLTKKLTALQTKQAQ
                     DRPVPSLQNP"
     gene            complement(30624..31916)
                     /locus_tag="ACFB64_000293"
     CDS             complement(30624..31916)
                     /locus_tag="ACFB64_000293"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC1208743.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTEGTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGSAREVQFFTDEDGERNLVALEKKGGIWRRSASDADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLLYDSLYFHGQQ
                     VAAGDILAAEVVKGDTTHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHS
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            31993..32961
                     /locus_tag="ACFB64_000294"
     CDS             31993..32961
                     /locus_tag="ACFB64_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208744.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSPHRQSCTASA
                     FYARYLWCGRLAQRPAQSLNMPSETVALATPVWQKMGLHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLNAHPVNHNRKKRGLPELNGLWLWDSLHGSAQGGTLFADTVWSRFH
                     PNRRALPDSFRAYAETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRTGKIKRLDIATDGQHGGTLTFKPTDRRKFWRCTKTFDGIW"
     gene            complement(33162..34631)
                     /locus_tag="ACFB64_000295"
     CDS             complement(33162..34631)
                     /locus_tag="ACFB64_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC1208745.1"
                     /translation="MVIFYFYFCGKTFMPARNRWMLLPLLASAAYAEETPREPDLRSR
                     PEFRLHEAEVKPIDREKVPGQVREKGKVLQVDGETLLKNPELLSRAMYSAVVSNNIAG
                     IRVILPIYLQQARQDKMLALYAQGILAQAEGRVKEAVSHYRELIAAQPDAPAVRMRLA
                     AALFEDRQNEAAADQFDRLKTEDLPPQLMEQVELYRKALRERDAWKVNGGFSITREHN
                     INQAPKQQQYGNWTFPKQVDGTAVNYRFGAEKKWSLKNGWYTTAGGDVSGRAYPGNKK
                     FNDMTAGVSGGIGFADRRKDVGLAVFHERRTYGNDAYSYANGARLYFNRWQTPRWQTL
                     SSAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWTGGLDFYRERNPADRGDNFNR
                     YGLRFAWGQEWGGSGLSSLFRLGVAKRHYEKPGFFSSFKGERRRDKESDTSLSLWHRA
                     LHFKGITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(34873..35130)
                     /locus_tag="ACFB64_000296"
     CDS             complement(34873..35130)
                     /locus_tag="ACFB64_000296"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC1208746.1"
                     /translation="MRAKTRFALRNGVNLQPFAAFNVLHRSKSFGMEMDGEKQTLAGR
                     TALEGRFGIEAGWKGHMSARIGYGKRTDGDKEAALSVKWLF"
     gene            35056..35217
                     /locus_tag="ACFB64_000297"
     CDS             35056..35217
                     /locus_tag="ACFB64_000297"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208747.1"
                     /translation="MQNIKSGKRLKIDTVTQSKTGFCPNAGADLPLTAAEQPDRRPSL
                     SVKPPFTPK"
     gene            complement(35314..35793)
                     /locus_tag="ACFB64_000298"
     CDS             complement(35314..35793)
                     /locus_tag="ACFB64_000298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208748.1"
                     /translation="MSFDCFGAFSYQPGSDTRPTNVGDTADFSFSDKPANGVFHYFSS
                     GKTDQKSSEALYDEINITGKNYNSGILAVDNMPVVKKYITDNYRTDLKQAVKKQLQDL
                     YKTRPEAWEENKKRVREEIIKQFGISEDVFNKLQQKMPQKINSLIEEQVLTPYILRP"
     gene            complement(36320..36565)
                     /locus_tag="ACFB64_000299"
     CDS             complement(36320..36565)
                     /locus_tag="ACFB64_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208749.1"
                     /translation="MLKSIELNSHIRNRLAAYLKGRGLDFQTAMQEEEGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            36677..37105
                     /locus_tag="ACFB64_000300"
     CDS             36677..37105
                     /locus_tag="ACFB64_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC1208750.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVLGAAIPFAAGRWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            37117..37593
                     /locus_tag="ACFB64_000301"
     CDS             37117..37593
                     /locus_tag="ACFB64_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC1208751.1"
                     /translation="MPIEIERRFLIENDKWRQYADEPLLLKQGYLSVEKERTIRIRIA
                     GKRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKRRYPVRWGGSLFEVD
                     VFLGENSPLVVAEIELPAENADFDRPDWLGREITSDGMFTNAYLSKHPFSSWKNAV"
     gene            complement(37623..38111)
                     /locus_tag="ACFB64_000302"
     CDS             complement(37623..38111)
                     /locus_tag="ACFB64_000302"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC1208752.1"
                     /translation="MLKITIIAACAENLCIGAGNAMPWHIPEDFAFFKVYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETVASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAERTERRVSSKGVAYTFVHYL
                     KG"
     gene            complement(38179..39234)
                     /gene="aroG"
                     /locus_tag="ACFB64_000303"
     CDS             complement(38179..39234)
                     /gene="aroG"
                     /locus_tag="ACFB64_000303"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC1208753.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDRRLLVIIGPCSIHDPKAALEYAERLLKLRKRYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAVEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIADQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANTKRMARAG"
     gene            complement(39379..39624)
                     /locus_tag="ACFB64_000304"
     CDS             complement(39379..39624)
                     /locus_tag="ACFB64_000304"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208754.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCPVPD
                     CRGNGKTDVPCGFARMNGGYCFYQSVFFFFEIKFLKY"
     gene            39852..40148
                     /locus_tag="ACFB64_000305"
     CDS             39852..40148
                     /locus_tag="ACFB64_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208755.1"
                     /translation="MRFKAEYARSKNDADDKRGNAHIANGAIGKMAAVYAGYTYTQPG
                     GESTRFRGGLERVQVKRYLGEKCCGYACPKSERFTLQADGEIRQNLGEGWFLKL"
     gene            40145..40384
                     /locus_tag="ACFB64_000306"
     CDS             40145..40384
                     /locus_tag="ACFB64_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208756.1"
                     /translation="MSGVAVYLRNKNKIAIASQDANLNSKGRFVSSGLNVGKQLTGSL
                     GVEFDPYYRHRAIRKSAEFVPNTTKTKTDSEKFNE"
BASE COUNT         9744 a        11436 c        10036 g         9449 t
ORIGIN      
        1 aagcgcacca ataatgggcg gaaatgaaaa tgccgtaccc tatgggcagg cttcgcactc
       61 tgaaaggttc aggcggcatt tttattggaa ggcttttttt tgcaaccgcc ttacacaaag
      121 cggttttttg tgtaagaact gctataatag ccgcctgtca tcgtcaggag cggctaatgc
      181 ctttaaaatt ccaaccaagg gaacgttcag ttatcatgtg cgactttcgc ggttatgaag
      241 aaccggaaat ggtcaagaaa cgccctgtcg tcgtcatagc gcgaaacagg cacaacggca
      301 aaccggtaac ggtcgtaccc ttaagcagca cagagcctgt ccctttggcg gactgccacc
      361 acaaaatgag tgaaaacccc ttaccggaca agccgcacat ccaatgtcgg gcaaaatgcg
      421 atatgacggc aacagtcgga ttggcacgat tagaccgata caaacccaaa gggcgcgacc
      481 gttgcatccc aataatcagt gaagaggatt ttcaggcggt taaaacagcc gttgccaagg
      541 cattcaaact gtactagaat aaaaccgttc ccttaaaggg gcttgcaaga ctgttccgaa
      601 atatgggcag ccgcgcacgg gcgacaggcg atgacaagcc gtccgtgcgt gtgatggggc
      661 gcggaatgcg ccccttgtcg tatctgcaaa cgcctacaaa tccccaatca tcccttcaat
      721 aaaaatgccg tccgaacctt caggcggcat tttccgttta ccttgccgca gccttgccgt
      781 aaatgccttc aagacatgcg gctatgcggc cggaggcgtc tttgtccgca tatccccgca
      841 acacttcgag ggctgcgacg gatcttttca ggattgaacg ggtttcgtgc caaaccgtcc
      901 acatcgtaac cgcctgtttg cagccgagct gcttcagcgg cgcggaaacg tctttgccca
      961 attcgatcat ccgtgtgccg taataaacca tagcggcaat gtcggccaaa gcgttgccgc
     1021 ttatgggcag ggcgggttgc ggcgcgggca acggttcgcg gtcggggact tcgccggcca
     1081 aacccgtgtg cagtgtcaat gcgtggacgt aggcgacggc ttcgggcagt ttgtcggcgg
     1141 ggatgccttc gacggcttcg acgttgaagc gttggtgtat catactgtac gcggaggagt
     1201 agcctatgcg tttgcgcccg acaagcgcgg caaccgcccg gcgcaatccg gtacggtcgt
     1261 cggcggtggt tttgggagta atttggtagc cgcctgtttt gcggatttga gggataacct
     1321 cttcaaatat ccagtcttgg aatttgacgg cttcggcttt gcgtgaacgg aagatgactc
     1381 ggtagagatt cggctcgttg atgaaaagta attcttggtt gccttttttt gttgcaacgc
     1441 tggatttttg aatgccctgg tcttttaatg gcaactgacg ggcattttga atttgaagaa
     1501 tttccgcaac atcgccaagg caaaaccaag gttcagcccc caagatttga gtacggacat
     1561 tttgagattt gaaagaaaat acagaaatgg tgttttgcat tttaagctcc acgaaagttt
     1621 gaaagcctct cttcgaccaa gaagaggtag gcggacgttt caggttggtc gactgctcgt
     1681 ggaaacagca catccggaga tgtcccgaaa gtccgcccgt tggctttttc agcaagggtg
     1741 tatgtttaac atacaccctt cagggaactc ttatcaaaac aaaagccgca agcgcggcaa
     1801 taaccacgaa agaaacaggc gaccaaacct gtgaaccgaa ctttactatt attccgctat
     1861 tgcgtcaata ttttgggggg aagcacggac ggagctttgt tgaaagttta aaacttggac
     1921 agtattcatg gtagaatgac ctttctattt catggtagat agacgcggaa agagagttgc
     1981 cgctctgctt tccacctgcc caaatcaagt tacagcttgg tttgggcttc ttttttatct
     2041 gcaatcagca agtgttgaaa ttataatttg tatctacgat tcatgcaagc catttctatt
     2101 attttttcag tttttctaat tcaagttcat gtttcagctt ttcttttacc catttggaga
     2161 agtctaaatt attggaaaat tccaataaat tagcttcttt ttcagtgtta aatgaaacgt
     2221 gctttaaaac gcgcttagat tcgtagcttt ttttggattg tttggttggg ttcattcttc
     2281 ccccttgttg aaaataatcg tagatacatt ttactcgatt taaaatcata gggcaacaaa
     2341 aaaaccgcca agtttgcggc ttggcggcgg tcgggctatt ttaagaactc ttttcggatt
     2401 ttttctttaa cccattggga aaaactaatt ttatttgcac cttatttgca ccttttttat
     2461 aaatataatt caaatatttg atttatatat taatttaatt aaatattaca tgaccctgca
     2521 taggggcgac ggaaaggcgg cgcgggggaa gggtgtgtgt attgtcgttc attgtcgata
     2581 tttcatcatt gcggactcaa tttcaatccg gaacaacctt catttttggg gaaatccgcg
     2641 tcatgaaaga gtccagcagt atggttaccg gcccgtcgtt gcagagcgac acctgcatat
     2701 gcgtgcggaa acgccctgtt tcgacatgaa tcccgtgtcc gcgtaacagc tccgccgttc
     2761 gcaggtaaag ccgttgcgcc tgttctgccg gcgcggcttg ggaaaacgaa ggccgccgcc
     2821 cgcttgccgc gtcggcataa agcgtaaact gcgacaccag cagcaccgcg ccgccgacat
     2881 ctttcaaaga caggttcagc ttgcccgctt cgtcttcaaa cacgcgcaaa tgtgcgattt
     2941 tgtcggcgat atagcgtgca tctttttctg tgtcgctatg cgttacaccg agtaacacga
     3001 caaacccgcc gtcgattttg ccgcaggttt ccgtgccggc ttcggacacg acatccacct
     3061 ttgcacctac cgttttctgt atgaccgcac gcataagccg tcctccttac ctgaacgtgc
     3121 aatgccgtct gaaaaggctt cagacggcat cggtatcgga aatgccatta cggtttgaca
     3181 ccgttttctt ccaaaagggc atcgattttc aagcgggctt tttcctgcct caaaccttgt
     3241 tccaactggt ttctgaccaa ctcgaaaggc tgcgcgtcgg ggtttttccc gaccgcgccg
     3301 agtttgaaca ggtaatagcg ttcgcccaat ttgaccggat tgcgggtaac gtcgccacgg
     3361 ttcataccgg caaactgcga agccagcggc tcgggaagct gctgcgccat aatgaaaccg
     3421 tcgaacgcct gctcgtcgtt cggataacgc ttcatcagcc cttcaaaaga cagccctttg
     3481 agcaggagct gctgcgcctg acgcgcctcc tcttcggttg cgaagctgac ctgctgcaat
     3541 ttgatcatgc ggatttgccg ctcataaaac tgacgcagtg cgctttcgga aaccgtttcc
     3601 gaacgttcca gaaaacggac gtactcctcg gcataaaaag acgcttcggc gattttaaag
     3661 cggttttgga catccttatc cttatccaaa ccttccttca atgccctgtt tttcaaaact
     3721 tccaaagttt gcagccggcg gacggcatcg tttcggattg cctgcccgtc cggtctttgg
     3781 gactgctccg catgccggtc tgcctgctgc atgatctgcg ccaccagcgt atccaccaaa
     3841 gccgggtcga tttcgggtgc tttggctgcc gcaaaacctg ccaacattgc agcaataact
     3901 gcggcagcgg tctttttctg tttcatggta ttccctgtcc tgcgcggcgg caaaacgcct
     3961 gtatgaacgt cttgccgccg tatcccgttt tcagattatt ttgcaggttt gatgtttgcc
     4021 ttacccaaca gcgcaccgac ggcacggtca atccgttccg cctgaaggtt gccggcaatc
     4081 tgtcctttca tttcgtcaaa agaaggcact ttcacctcgc ggctgtcgtt gacataataa
     4141 acgccgtaga aatcgccgtt tttcagcggc gttgccgtaa attcgccttt tttcaagtcc
     4201 ttaattgcct gataaagcgg cggaacaccc tgttccaaat ctttcagcgg cacatatccg
     4261 tccggcgcgc cggtccgttt ggtgcggtcg ttgagcgagt attgtttcaa aacggcatca
     4321 aaaccttttt tcgccttcaa atcggcaacc gcttttttcg cattttcttc cttgtcggtc
     4381 aggatttcgc ccaactggac ttcctgcgtg cctttataaa aaccgctgat attgtcgtaa
     4441 acggctttta cttcctgctc ggaaaccggt tgggttttgg cgatatgcag tgcgtatgcc
     4501 tcgccgttca agccatattt taccgcctgc caaacggttt tgaaggacgg tttcttgtcg
     4561 tcgcccgact ttttcgcttc ggcacgcaat ttggcaagcg catctttaaa ctctgccgac
     4621 cggtcgagtt tcaggcgttt cacttcctgt gcgaccacgg tgttgaccac ttcgttttcc
     4681 agcagggatt ggcgcagttg cggcgtgtct tcggcacggc tgttttccgc acggaatgcg
     4741 gcaacctgcg catcgatgac ggaactgtcg attttctgac cgttaacggt tgccagcgtt
     4801 tgggcaaaca ggctgccgga acaggcaagc agcgcaacgg aagtcaggat ttttgctttc
     4861 ataatcttct ctctttaaat tttaaattga aatattcagg cagacttcat tcagtccgcc
     4921 gtatggaaat actcgtcagg ggtagccgcc cggatgccga gcgcgtgaat catgccgcct
     4981 gaaaataaat ctttgagcag cgatttgacc gttttctgac ggttcagacg gcttaaacct
     5041 tcaaaacggc cgctgacgac caaaacggca taatgtccgc cgccggtatt gcccgcgtgt
     5101 cctttgtgca gatggctctc atcgccgatt tccaacacca gaggatcgag cgtctgcagg
     5161 cgttcgcgga tcaaatcgac agccggcatt atttgaacac cgctttaaac ggcttgatca
     5221 gcacttcgct gtacacgccg gcatgaacat agggatcgtc ttcagcccaa gcctgcgccg
     5281 catccaaaga ctcgaactgt gccacaatca agctgcccga aacacgttcg ggattgtccg
     5341 gcagcaggtt tgggcctgcc gtcagcagac ggccttccga tttcagcgtt tccagccgtt
     5401 taaagtgttc gggacgtgcc gccatacgcg cttcatgcac atcctccccg tctgttgcca
     5461 gcaacataaa atattccaca gtcaatcctc ctttttcaga taggtactca gataaatacc
     5521 ctgaataata aagaaaaaaa gcatcagcgc agtcgaaccg aacatcttat agttaaccca
     5581 ttgcgcttca aacctagtaa acacaaacca gttggcaata cccataaaaa tcagaaaacc
     5641 gacccacata tatgtcaatt ttccccatac ggcatccgga agctgaatct ccctgccgat
     5701 actcgctttc aagccgtttt tacccgcaag gtggctgccc agcaggaata acgccccgca
     5761 ccagaacaat actgtcggct tccacataat gaagcggctg tcgcccaaaa caatggttgc
     5821 gccgccgaat acgacaatca gcaccagtcc gacccactgc atcgtatcca gccttttatg
     5881 cttccaatac aggaaagccg cctgaaccac gcctgcaacc aaggcaaccg ccgcagcggc
     5941 aatcatattt ttggtaacgg tataagtagc aaaaaacaag atgacggaca aaaggtcgct
     6001 gacaaatttc atatccaaat gagaaagcaa atcaaataaa cgctaatcat acaggcagaa
     6061 ccctatcttt gcacctgcaa aaccaacaaa ttttgttttg attaatgcaa atatgcacag
     6121 atgtttttga aaaaagatgg aaatatgtca taatttataa aattggcttt aaaatacagc
     6181 ttcggtcgga acctctgcct atccgcaccc tatcgtccgt attgcctatg cctgatgacg
     6241 aaatttacag actgtacgcg gtcaaaccgt attcagccgc caacccacag gggatacatc
     6301 ttgcaaaaac aacagacaaa tcaaactgat tgccgcctcc atcgcagttg ccgcatcctt
     6361 tcaggcacat gcgcgactgg gcggactgaa tatccggtcc aaccttgacg aacccttttc
     6421 cggcagcatt accgtaaccg gcgaagaagc caaagccctg ctaggcggcg gcagcgttac
     6481 cgtttccgaa aaaggcctga ccgccaaagt ccacaagttg gacgataaag ccgtcattgc
     6541 cgtttcttcc gcacaggcag tccgcgatcc cgtcttggtg ttccgcatcg gtgcaggcgc
     6601 gcaggtacgc gaatacaccg ccatcctcga tcctgtcggc tgctcgccca aaaccaaatc
     6661 tgcactttca gacggcaaga cacaccgcaa aaccgctccg aaagcagagt cccaagaaaa
     6721 tcaaaacgcc aaagccctcc gcaaaaccga taaaaaagac agcgcaaact cagccgtcaa
     6781 accggcgcat aacggcaaaa cccataccgt ccgcaaaggc gaaacgctca aacagattgc
     6841 cgccgccatc cgtccgaaac acctgacgct cgaacaggtt gccgatgcgc tgctgaaggc
     6901 aaacccaaat gtttccgcac acggcagact gcgtgcgggc agcgtgcttc acattccgaa
     6961 tctgaacagg atcaaagcgg cagcacccaa aaaaatcaaa gcggaacaac ccaaaccgca
     7021 aacggcgaaa cccaaagccg aaactgcatc catgccgtct gaaccgtcca aacaggcaac
     7081 ggtagagaaa ccgatagaga aacctgtcga aaaaccggtt gaaaaacctg aagcaaaagt
     7141 tgccgcgccc gaagcaaaag cggaaaaacc ggccgttcga cccgaaccca aacccgcagt
     7201 gtccgaaact ccggcttcgg cgacagaacg ccaacccgga cctgtacccg ctgcaaatac
     7261 tgccgcatcg gaaaccgctg ccgaatccgc ccccccaaga agccgccgct tctgccatcg
     7321 acacgccgac cgacgaaacc ggtaacaccg tttccgaacc tgttgcggaa acagccagtg
     7381 aagttgccgc cgaacccgtt gccgaacctg tcgaacaggt ttctgccaaa gaagaaaccg
     7441 aaagcggact gtttgacggt ctgttcggcg gttcgtacac cttgctgctt gccggcggag
     7501 gcacggcatt gatcgccctg ctgctgcttt tacgccttgc ccaatccaaa cgcgcgcgcc
     7561 gtaccgaaga atccgtccct gaagaagagc ctgaccttga cgacgcggca gacgacggca
     7621 taaaaatcac ctttgccgaa gtcgaaactc cggcaacgcc cgaacccgct ccgaaaaacg
     7681 atgtaaacga cacacttgcc ttaggtgggg aatctgaaga agagttatcg gcaaaacaaa
     7741 cgttcgatgt cgaaaccgat acgccttcca accgcatcga cttggatttc gacagcctgg
     7801 cagccgcgca aaacggcatt ttgtccggcg cacttacgca ggatgaagaa acccaaaaac
     7861 gcgcggatgc cgattggaac gccatcgaat ccacagacag cgtgtacgag cctgagacct
     7921 tcaacccgta caaccctgtc gaaatcgtca tcgacacgcc cgaaccggaa tctgtcgccc
     7981 aaactgccga aaacaaaccg gaaaccgtcg ataccgattt ctacaacaac ctgttctcaa
     8041 acaaccatat cggcacagaa gaaacagctt ccgcaaaacc tgccgcaccc tccggactgg
     8101 caggcttcct gaaggcttcc tcgcccgaaa ccatcttgga aaaaacagtt gccgaagtcc
     8161 aaacaccgga agagttgcac gatttcctga aagtgtacga aaccggtgcc gtcgcggaaa
     8221 ctgcgcctga aacgcccgat ttcaacgccg ccgcagacga tttgtccgca ttgcttcaac
     8281 ctgccgaagc accggccgtt gaggaaaatg cagcggaaat cactttggaa acgcctgatt
     8341 ccaacacctc tgaggcagac gctttgcccg acttcctgaa agacggcgag gaggaaacgg
     8401 tagattggag catctacctc tcggaagaaa atatcccaaa taatgcagat accggtttcc
     8461 cttcggaatc tgtaggttct gacgcgcctt ccgaagcgaa atacgacctt gccgaaatgc
     8521 atctcgaaat cggcgaccgc gatgccgctg ccgagacagt gcagaaattg ctggaagaag
     8581 cggaaggcga cgtactcaaa cgtgcccaag cattggcgca ggaattgggt atttgattcc
     8641 caactgccct ttcgcagatc aaggatgccg tttcagacgg catctttttt tgccttatcg
     8701 gtgtaacgga taaagtttga accggcacag gctcaaacag caggccgacg gcaacaaaat
     8761 gccgtccgaa acccctaaag gcttcagacg gcattggcgg cggcggtatc aggtattcgc
     8821 ataggcaacc gaatcgtcgc cgcttttctt ttgaacgaac tcgattttgc atccgtcggg
     8881 gtcttctacg aaggctatca cggttgtgcc gtgtttcatc aggccggctt cgcggacgac
     8941 gtttccgccc tgccgcttca cgcgttcgca ggcttcgtag gcatcgtcca cttcaaccgc
     9001 gatgtgtccg taggcgtcgc ccaagtcgta tcgttccgta tcccagttgt gcgtcagttc
     9061 caaaaccgtg ctgtcggttt cgtcgccgta accgacgaag gcaagggtaa atctgccttc
     9121 gggatagtct tttcggcgga gcagtttcat acccaaaacg ttttggtagg aatcgaggga
     9181 tttttcgaga ttgcccacgc ggagcatagt atggagtaag cgcatttttc gtgttcctct
     9241 cggtggtggt taaacttcga ttttattcgg cgtaaacgtc tgccatttgt tgcaggcggg
     9301 acagtgccag aaaaagactt gggatttgaa gtggcagttg cggcaacggt acatcacgct
     9361 gcgctggagc tgccgtccga taaccgaacg catcatgtcg gcatcggctt tccaagccgg
     9421 atccaaatcg ctgagtttca aaccgagcag gcggtacacg ccgttaaggt cgggcttgcg
     9481 gcggacaagc tcgacggcgg tttgcgcggc ttctttctcg cccttaagca gcagggattt
     9541 ctcgtacacg acattgatca ggtcaagttc gggaaacgtc tgcatatatc ctgtcagacg
     9601 gttcaagcct tcttcaggtt ttccctgcgc ggcataggct tcgtaaagct tctcgccgac
     9661 catgctcaag tatgcatggt tttgctgctc gatggcggca taggcttcga cggcggcagg
     9721 gaaattgcct tgtcggtgtt caatgtcgcc caaaatcatg ttggcgcggg tgcatttttt
     9781 gttggcttcg agtgccttgc cgacattgaa acgcgcggca tcgaaattgg acttgaacag
     9841 cgcggcttgg gcaagttcgc aataaaactg tgcaatctca aactgatatg tctgttcgtc
     9901 gtgactaaga agttgggcgg tttcaaccgc tttttcccaa tccctgtcct gctggtagat
     9961 attgagcagg tgctgtctgg cttcacgcgc catttcaccg tcttgcagcc ccaaaaaaat
    10021 ctgttcggca cgatcgacca aacccgcgct ttggtagttt tgcgccaatt caaacaggac
    10081 gcgcgcgcgc ttttcgccga ccgtatcggg agaatcgagc attgtccggt gtatgttgat
    10141 ggctttgtcg ttttcgccgc gctgacggta aagtttgccg agggtaaggt tcaaatcata
    10201 cgattgcggc cggccgtcga cgacttccgc caactccctt gccgcgcgcc cgctgttgcg
    10261 gtcgaccaaa gcgtccaggc ttttataaaa tcccgaaggg atgctttttg cctgcttcaa
    10321 tacggttttc atatccacgc gggcggcaaa ccagcccatc gtgaagaaga cgggcaaaag
    10381 gataatcggc agcaggataa tccacaattc gttgtccata tcggctttct taaggctgtt
    10441 tggcagattc ggcagcattt tgtatcggcg gtgcagtcaa tttctgtccg ctcaagcgcg
    10501 cacttttctt cacttccgca cgcaggcggc tgttttcgcc gcgcaaggac agcagccgcc
    10561 cgaacagggc aaacattccg aacacgatgc cgacgacaaa cgcgccgaac aatacgacaa
    10621 tcagcggcag attgacactc tgccccggaa gataggaaaa ggtaacggca tccatattaa
    10681 tgacggcaag cagcaggaag agcagcagga taatgatttt gatgacggta tagataagtt
    10741 tcatgtttgc tccgtaaata aaaacgccgc acggacagct tcgcactctg aaccaagatt
    10801 gcgttagttt aaacgatttg cacggtttcg gatagaatgc ggcagcgtgt tcggacggca
    10861 tacggagtat gcgattgccg acaattttac caaatacacc gctcctttcc atttgaaaaa
    10921 taacggattg gacaccgcat cgacagaaaa acccgccgcg cacttgtcaa aaccctgttt
    10981 gcaggcgtat ctttacaacc ttcaaattca aaccgttcat tgaaacatat cagaataaga
    11041 aaggctttac atcatgagca gacccgtacc cgccgtattc ggcagcgttt ttcacagtca
    11101 aatgcccgtc ctcgcctacc gcgaaggcaa atggcagccg accgaatggc aatcttccca
    11161 agaccttacc cttgcacccg gcgcgcatgc cctgcattac ggcagcgaat gctttgaagg
    11221 tttgaaggcc ttccgtcagg cagacggtaa aatcgtgctg ttccgtccga ccgccaatat
    11281 cgcgcgtatg cggcaaagtg cggacatttt gcacctgccg cgccccgaaa cccaagctta
    11341 tctcgatgct ttggtcgaac tggtcaaacg tgccgccgac gaaattcccg atgcgcctgc
    11401 cgcgctgtac ctgcgcccga ccttaatcgg taccgatccc gttatcggca aggccggttc
    11461 tccttccgaa accgccctgc tgtatatttt ggcttccccc gtcggcgact atttcaaagt
    11521 cggctcgccc gtgaaaatct tggtggaaac cgaacacatc cgctgcgccc cgcatatggg
    11581 ccgcgtcaaa tgcggcggca actacgcttc cgccatgcac tggttgctga aggcgaaagc
    11641 cgaatacggc gcaaatcaag tcctgttctg cccgaacggc gacgttcagg aaaccggcgc
    11701 atccaacttt atcctgatta acggcgatga aatcattacc aaaccgttga cagacgaatt
    11761 cctgcacggc gttacccgcg attccgtgct gactgtcgcc aaagatttgg gctataccgt
    11821 cagcgaacgt aatttcacgg ttgacgaact gaaagctgct gtggaaaacg gtgcggaagc
    11881 gattttgacc ggtacggccg ccgtcatctc gcccgttact tccttcgtca tcggcggcaa
    11941 agaaatcgaa gtgaaaaacc aagaacgcgg ctatgccatc cgtaaggcga ttaccgacat
    12001 ccagtacggt ttggcggaag acaaatacgg ctggctggtc gaagtgtgct gatgctttaa
    12061 ataaaaaaaa tgccgtctga aacccgtttg gcgtttcgga cggcattttc gcgttcggac
    12121 cgtttccgcc gcacctgcag caagtcggta caaaggcaat cggttaaaac aagcgtccgc
    12181 atttcccatc ccgcctgccg caagtcgggc atttccctgg aaatacgctt caaacggcaa
    12241 accgccgccc gaaaccgata ctcggcacgg acggcgcacg aatttgaaaa cggcggatta
    12301 tccctcggtg ctcaaggcat tgatgctgta accgccgtca acataagtga tttcgccggt
    12361 aatgccggac gacaggtcgg acagcaggaa ggcggcagta ttcccgactt cttcgatggt
    12421 aacgttgcgg cgcagcgggt tgtgggcggc gacgtgtccc aagagtttgc cgaaatcggc
    12481 gatgccggag gcggcgagcg ttttgatcgg gccggcggaa atgccgttgc agcggatgcc
    12541 ctctttaccc agacaggcgg cggtaaagcg gatgcctgcc tcaaggctgg cttttgccat
    12601 acccatcacg ttgtaattcg gaatcgcgcg taccgcgccc aagtagctaa gggcgacgat
    12661 ggcggagttt ctgccgcgca tcatcggacg ggcggctttt gccaatgcgg gcaggctgta
    12721 tgcggaaatt tcgtgtgcgg tgttgaacgc ttcgcggctg atgctgtcga ggaagtcgcc
    12781 gctcaaggct tctttcggcg cgaagccgat ggaatgcacc aaaccgtcca agccatccca
    12841 atgtttgcct aagtcggcga acacttggtt gatttcgtcg tcgctggcga catcgcagcg
    12901 gaatacgagt tcggaatcta attccgccgc cattttgcgg acgcgctctt ccagtttgtc
    12961 cacaacgtag gtaaacgcca gttccgcgcc ctgttcgcgg caggctttgg cgatgccgta
    13021 agcgatggaa cgctcggaaa tcatgccggt aatcagaatt tttttgcctt gcagaaaacc
    13081 cattttgtat ccttcaaata gtgttcggtt cgttctaaac cgttgattat agcaaattgt
    13141 ccctgtttct gtgttttcac gttgcagcgt gcggacggca atgccgtccg aagcggattt
    13201 cagacggcat tggacgtttc aaatacggtt taaggcatca gatgccgcgc aagagttcgt
    13261 tcacgctggt tttggcgcgg gtttgcgcgt ccacgcgttt gacgatgacg gcacagtaaa
    13321 ggctgtggct gccgtctttg gaaggcaggc tgccggacac gacgaccgaa cctgccggta
    13381 cgcggccttg gtagatttcg cctgtggtgc ggtcgaagat tttggtggat tgaccgatga
    13441 acacgcccat cgaaatcacg ctgccttctt cgacaatcgc gccttcgacg atttcggaac
    13501 gcgcgccgat gaagcagttg tcttcaatga tggtgggtgc ggcctgcagg ggctcgagta
    13561 cgccgccgat gccgacaccg ccgctcaagt ggacgttttt gccgatttgc gcgcaagagc
    13621 cgacggtcgc ccaagtatcg accatcgcgc cttcgtcgac gtatgcgccg atgttgacgt
    13681 aagacggcat cagcacggcg tttttggcaa caaagctgcc gcgtcgggca accgcacccg
    13741 gaactgcgcg gaagcctgcg tttttgaact cgtcttcaga ccagtcggca aatttggtcg
    13801 gcactttgtc gaagtatttg ttcacgccgt cgttgaggac ttcgttgtct tggatgcgga
    13861 aggacagcaa cacggctttt ttcgcccatt cgttgacttt ccactcgccc acgcccaagc
    13921 gttcggcgac gcgcagtttg cctgaatcga gttggcggat ggtttccaac acggcttctt
    13981 tgacttcggg ggcaacggtg gtcggagtga tgtccgcgcg gttttcaaag gcggtttcga
    14041 taatgttttg caaagacata atgtttcctt ggttcgagat atttcgggac gcttgccggt
    14101 attgttcaga cggcatggcg gttggaacaa acgtgctatt gtgccataca aacgcccgcc
    14161 cgtcagccgc gccgcaggct tgccggatgc cggcgtttcg gaatcgggcg ggcggcaggc
    14221 atatgtcttt ttccgcgccc gaatcaatac agaaaggcgg cggtactttt atgccgcgcc
    14281 gccgcctttc agacggcatt cgcggtaaaa cgccatcagc ccttcggtcg aggcatcgtg
    14341 tacggacgtg ccgccttcca gttcgccgat gatggttttt gccaactgtt tgccgtactc
    14401 aaccccccac tgatcgaagg ggttgacgtt ccatatcgcg ccttggacga aggttttgtg
    14461 ttcgtaagcc gccatcagca tacccagatt gcagggcgtg aggcggtcga gcaggatgct
    14521 gttgctgggg cggttgccgg ggaactcttt gtgcggcgcg aggcgttcgc gttccgcttc
    14581 gggcaaatct gccagttcgg cgcgtgcttc gtctaaggtt ttacccttca tcagggcttc
    14641 cgcttgggca aaggcgttgg caacggtaaa acggctgcgt ccgtcctccg cgccctgcgc
    14701 cgtcatcggg acgataaagt cgcagggaat caggcgcgtg ccttggtgga gcagttggaa
    14761 ataggcgtgc tggcagttga ccccttcacc gccgaacacg atgccgcccg ttttgcacgc
    14821 ggcgggactg ccgtctgaag cgcggctttt gcccaaactc tccatatcga gctggttcag
    14881 ccacgccggc agcaggcgca ggttgtggct gtacggaacg gcggtctgcc cgtccgcgtg
    14941 ctggaaattg ttataccaca cggcaatcag tgccatcaga acgggaatgt tgcgacgcgg
    15001 gggcgtgtgg aaaaaatggc tgtccatcgc gtgcgccccc gctagcagtt cgcggaaacg
    15061 cgccccgcca accgcaacca tcacgggcaa accgacgggc gaccagacgg aatagcgtcc
    15121 gcccacccag tcgtacatcg caaacacgcg ttccgccgca atgccgaaac tttgcgccgc
    15181 ttccgtatcg gcagacaccg cgcaaaaatg gcgcgccgtt tcggattccg agaaccccgc
    15241 accgcgatac cacgccttga ccgcctctgc attgagcagg gtttccggtg ttttaaagga
    15301 cttgctggca acgcaaaaca ttgttgtttc ggggttcaga cggcataaga cctcatccag
    15361 gcaggcaggg tcggcattag aaacaaaatg gacggaaatc tgccgtctga acggctcgag
    15421 cgcctgcacg cacattgccg gcccgaggtc ggatccgcct atgccgatgt ggacaaaatc
    15481 cgcaatccgt tttccggtta tcccctgata taaaccgtcg tccaaactgt gtgcaaactt
    15541 caacgcacga tttaactcgc ggcggatttc gggcaacacg tccctgccgt ccgcataaac
    15601 ggcatccgca ccgtcgggca ggcgcaaagc cgtatgcagc gcggcacgcc cctcgctgcc
    15661 gttgactttc gcacccgtcc gcaaagcacg cattttccct tccaaatccg ccgtctccgc
    15721 aagacggcag agcagttgca gcgtatcttc gccaaaacgg tttttgctgt aatcgaacaa
    15781 catcccgtcc aaacgctcat gcatacgctc aaaacggtcg ggttcggcag caaagcggtc
    15841 gcgcaaaagg atgtggcacg tatcctgata atggcgttcg agcgcatacc atgcacgggt
    15901 aaaggcatcc atctgttttc cttgattttt cagaaccgga ttaaaatata gcagaatgta
    15961 gtctaacaaa cggcagacac tttagcgaat ctcaggaaca ccgcccgccg cgtccaataa
    16021 caccttattt ccatatggcg aaaatatagt gaattaacaa aaatcaggac aaggcggcgg
    16081 gccgcaggca gtacggatag tacggaaccg gttcgcttgg tgcttcatca ccttagggaa
    16141 tcgtcctctt tgagccgggg cgggccaacg ccgtaccggt ttttgttaat tcactatacc
    16201 ctccggccgg catttgaccc gcaacaatat cctgcaagat ttattgtgtg cgcataaatg
    16261 ccggacagcc gcctaaatta aggtataatc gcatccgatt ctgtcccgtt tgccgcccgc
    16321 aggcgggatg tcggcggttt tcatcttacc gggaacacgc cgatactgtt ttttaacgat
    16381 gtttcttaca ttttattcca attactttac ggggctagaa tatggctaaa aatgggggat
    16441 tttctttgtt cgcaaagaaa gaaaaacgct ttatctttga aggcaggcat tctgcctccg
    16501 acaaactggt caacggcgaa gtatccgcgt ttaccgaaga agaggcacgc aaaaaactgg
    16561 caaaacgcgg catccgcccg ttgcagatta cccgtgtgaa aacaagctcc aagcgcaaaa
    16621 tcacacaaga agacattacc gttttcaccc gccagctttc cacgatgatt aaagcgggcc
    16681 tgccgctgat gcaggcattt gaaatcgtgg cgcgcggaca cggcaacccg tctatgacgg
    16741 aaatgctgat ggaaatccga ggccaagtgg aacagggcag ctcgttgagc cgcgcattct
    16801 caaaccaccc gaaatatttc gaccgcttct actgcaacct ggttgcggcg ggcgaaacgg
    16861 gcggtgtatt ggaaagcctg ctggacaaat tggcaattta caaggaaaaa acccaggcca
    16921 tccgcaaaaa ggtaaaaacc gcgctaacct atccggtatc cgtcatcgcc gtcgccatcg
    16981 gtttggtatt cgtgatgatg attttcgtac tgcccgcctt taaagaagtt tacgccaata
    17041 tgggcgcgga gcttccccca ctgacccaaa cagtgatgga tatgtccgac tttttcgtct
    17101 catacggctg gatggtgctg atcgcactgg gctttgccat atacggcttc cttaaattga
    17161 aggcgcgttc gattaaaatc caacggcgta tggatgccat actgctgcgt atgccgattt
    17221 tcggagacat tgtccgcaaa gggacgattg cccgctgggg caggacgacg gcaacgctgt
    17281 ttgccgccgg cgtgcctttg gtcgatgtat tggactccac tgccggcgcg gcgggcaatt
    17341 taatctatga agaagccacc cgggaaatcc gtacgcgcgt catccaaggt ctgtctatga
    17401 cttcggggat gcgtgcgacg gaactgttcc ccaatatgat gttgcagatg tcctccatcg
    17461 gcgaggaatc gggttctttg gacgatatgc tcaacaaagc cgccgaattt tacgaagacg
    17521 aggtggacaa tgcggtcggc aggctgtccg ctatgatgga gccgattatt attgtgattt
    17581 tgggcttggt catcggtacg cttctggtcg ctatgtatct gccgctgttc aacttgggca
    17641 acgtggtcgc ctgatttgcc gcacagatcc ggcgcggatt ggttctgcgc cggtttgttt
    17701 ttgctttgaa tatatcaagg acaaaatatg tctgatttgt ctgtattgtc gccgtttgcc
    17761 gtgcctttgg cagcagtttt ggggctgctg gtcggcagct tcctgaatgt cgtcatttac
    17821 cgcgtacccg ttatgatgga acgcggctgg acggtatttg ccaaagaaca tttaaacctg
    17881 ccgctgaccg acgatgaaag ccgtaccttc aacctgatga agccggattc ctgctgtccc
    17941 aaatgccgtg tgccgatacg cgcgtggcag aacatcccga ttgtcagtta cctgctcctg
    18001 cgcggcaaat gcgcttcctg ccaaaccaaa atcagcatac gttatccctt aatcgagctg
    18061 ctgaccggcg tattgttcgg gctggtcgcc tggcaatacg gctggtcttg gattacgctg
    18121 ggcggtttga tactgaccgc gtttctgatt tccctgacct ttatcgatgc ggacacccaa
    18181 tacctgcccg actcgatgac attacccttg atctggctgg ggctgatatt taatttggac
    18241 ggcggcttcg tgcctttgca gtctgccgtt ttaggtgcgg ttgccggcta tagttcatta
    18301 tggctcttat gtgcagtgta taaactgctc acaggaaaaa ccggtatggg caacggagat
    18361 ttcaaactga ttgccgcatt gggcgcgtgg ctcggcatat ccgcattgcc cgtgctgatt
    18421 tttgtttcct ctctgatcgg tttggtcgcg gcaatcgtta tgcgcgtcgc caaggggcgg
    18481 cattttgcct tcggccccgc actgacagtt tcgggctgga taatttttac ggcaaacgat
    18541 tccgtatggc gggcggtcaa ctggtggctg acccatccgg tgagatgaca gcatgggtcg
    18601 gactgaccgg cggaatcggc agcggcaaat cggcagccgc gcaatatttt gccgatttgg
    18661 gcgtgccgcg catcgatgcg gacgcggcgg cgcactcgct gacggcttca gacggcatcg
    18721 ccctgccgga aatcaggcgg ctgttcggcg acaccgtttt cgacacacag ggtttgttgc
    18781 ggcgcgacat attgcgtaaa gaaatctttg cctccccatc gcgcaaagcc ttgctcgaat
    18841 ccgtgatgtt gccgctgatt ttctcagaaa tcaaaaaaca gcaagaaacc tttaccgatg
    18901 cggtttacgg cattgtcgaa attccgctgc tgacggagaa gcgtcaattt atcagcctga
    18961 tacggcgtgt cctgaccata agcgcacctt tggaaaaacg tatcggcagg gtaatggcac
    19021 gcagcgggct gacgcgcggc gaggtcgcgg acatcatcag ccatcaggca tccgaatccg
    19081 aacgcctgct gcttgcagac gatgtactgc tcaatgacgg cagcctcaaa agcctacgtg
    19141 agaaaacaat gctcctgcac gcgttttatt cagggatttt cgcctcaaaa ccaacacaag
    19201 gaaaacacaa tggctgaatc gcggcaaaca cgccttcaag tcaaatgtcc gacctgtcaa
    19261 acggcagtag tatggaaacc ggaaaacgca ttccgcccgt tctgttcgca acgctgcaaa
    19321 ctgatcgact tgggcggatg ggcagacggg aaatatacgg tttccggcca aacggaaagt
    19381 ttgccggaaa tatccgaacc cgacggggca taccgctgac cgccccgcct tcccggcaaa
    19441 caccctgaaa ggtcaaatgc cgtctgaaac aaacacgctt cagacggcat tttcattctc
    19501 aaacctaatc gttggtattt gccgttacct cttccaatga agtaatgccc tgcataattt
    19561 tcaaaatacc ggcccggcgc aaatccacca taccctcctt ataggcaacg tccaaaatac
    19621 ccacttccgt accgttgttc ataatcacac gctgcatttc ttcgctgatg ggcataacct
    19681 catacacgcc cgcacgcccc ttataaccct gcccccggca acggtcgcaa ccgacggcgc
    19741 ggtaaagttt ccaatctttt gcaagatcct catcggtgaa accgacttcc ttcaaagcag
    19801 aggcagacgg gcgttccact tcctgtttgc agctcgaaca cagcctgcgt aaaagacgct
    19861 gcgccataat caggctgacc gaactggcaa tattaaacgg cgcgacaccc atattcagca
    19921 tacgcgacaa cgtcgccggc gcattattcg tgtgcagtgt ggaaaacacc atatgccctg
    19981 tttgtgccgc cttaatcgca atatcggcag tttccaaatc acgaatctca ccgaccataa
    20041 tgatgtccgg gtcctgacgc aggaaagact tcaaagcagc ggcaaaagtc agaccctgct
    20101 tatcattgac gttaacctga ttgatgcccg gcaggttaat ctcggcaggg tcttccgccg
    20161 ttgcaatatt taccgactcc gtattcaaaa tattcaaaca ggtatagagc gacaccgtct
    20221 tacccgaacc cgtcgggccg gttaccagca ccatcccgta aggacggtga atcgcttcca
    20281 acaacaattt tttctggaac ggctcaaaac cgagctggtc gatgttcaaa gacgcggcat
    20341 cggaattcaa aatccgcatc acgacctttt cgccaaacag cgtcggcaat gtgctgacac
    20401 ggaaatcgac aggcttgccg cccttttgaa aggtcagctg catcctaccg tcctgcggta
    20461 tccgtttttc ggaaatgtcc aaacgcgaca ttaccttaat ccgggaagca agctgccccc
    20521 ttaccgcaat gggcggctga accacctcgc ggagctgccc gtccacacgg aaacggatac
    20581 gcgcattgtg ttcgtaaaac tcgaaatgga tgtcggatgc cccgctacgc aaggcatccg
    20641 acaaagtttt atggataaac ctcggaacag ggccgtcttc tgcctcctcg ttgtcgatat
    20701 acagggtgtg gctttcctct tcctcttgcc cctccccaag ctcctgaagc agcgatgtcg
    20761 aacgcgaacc cacccaatcg agcaaacccg ccaactggtc atcctcgaca atgaccaact
    20821 caaccgcaat ccctgcggca gaaacggttt tctgaatttg cggcatctgg gtcggatcgg
    20881 aaaccgcaaa aaatactttg tcgccccgac ggaaaaccgg cacacagtgg aactccacca
    20941 tctgctcctc cgtcaacacc cccatcagca ccctgtggcg cggataatga cgcaaatcaa
    21001 gaatcgaata actgaacacc ctcgcaatca atgccgcaag cgacttgggc gaaatgacac
    21061 cgtctgaaaa cagcatcggc aacacttcct tacccgcctg cgactcattg tagtaatgct
    21121 cggcctgctc aacagtaacc acctggtttt gaaccagaat cctcagcaaa cctacgctca
    21181 tacgacctta tccccaaatt tattcattgt tatacctgta cagattttat cataaaacaa
    21241 cagatttcca agcctgaaca tcttttacga agcctgaaaa tcatttcacg attctgccgt
    21301 cctaaaggtc ggtttttcaa gcaggaagaa aaagttttca gatggcaaaa aagccctcca
    21361 gcactgaaag gccttatatc ggaaacttcc cgcaacacgg gaaacagaca aatgcaaacc
    21421 gtcaaacctc gccaacagga atcgaacctg tattttacgc ttaggaggca tacgttctat
    21481 ccgttgaact atggcgagcc gaaatgaaaa gggagatttt aaacctttcc ggcaacaaaa
    21541 gacaagatgt cggccgcaat cagcggcccg acagggatac cgcccataaa tgccaccccg
    21601 atgaccgtac cgatcagcaa gcccgttacc aataccggct gccgtcccat caaaggcacg
    21661 ccgcatcccg ccagccaagc cacgaaaata ccgataaaaa cggcggatat cattttaaaa
    21721 ttcaaaaatt cggcaacagg aggaacctgc gcctttcctg aaaccaacgg actcaaaacc
    21781 cctatggtca aaagaatgat accgaggttc aacccgtgct tctcgaccaa cgggacaaac
    21841 tgtaccaatg ccgtctgctg catcagcagc aatatggttg ccgagacggt aatcgaattg
    21901 ttgttgctga ccacccccaa cagaatcagc gtaaccagaa acaggggaac aaaactgaaa
    21961 ttcatcggtt acgacaccct gaccaaagat tccttcgcca gctgaatcat agcatccttc
    22021 acttcgctgt cggacagggc atccaacgcg gcaacggcac aatcgactgc tttgcgcgcc
    22081 tcgcctatcg aatatgccaa agcatccgaa cggacgacat aatcgcggat tttttcaaaa
    22141 tagccgcgat ctgcattttc caaagcagta cgcacatcgt tcgcaacctg ttcggaaccc
    22201 tgacgcatca gataaatcaa aggcagggtc ggttttcctt ccgccaaatc gtcgccgacg
    22261 tttttacctg tttcttcggt ttcccccgaa taatccagca catcgtcaat aatctggaat
    22321 gccgtaccga cgtacatacc gtagtctttc aaggcctgtt cgtgcccggg ggaagctttg
    22381 cccaaaattg cgccgacttg agcggcagct tcaaacaatt ttgccgtttt atattggatg
    22441 actcggatat attgttcttc ggtaatgtcc gtattgccga tgttcatcag ctgcatgacc
    22501 tctccctcgg caatgatgtt ggtcgcatcc gccatcactt ccaaaacgcg catactgccc
    22561 gaggcaacca tcagttgaaa tgcacgggta tataaaaagt cgccaaccaa cactgccgcc
    22621 gcattgccga acagattgtt tgccgttgcc cgcccacggc gcaaatcgct ttcatcgacg
    22681 acatcgtcgt gcaggagtgt ggaagtgtgg ataaactcga ccatcgctgc cagcgaatac
    22741 agtttctcgt catcataacc gaccgcctta cccgccaaaa tcgtcataat cggacgcagg
    22801 cgtttgccgc ccgcgctgat gatatatgta ccgatttgcg aaatcagtgc gacatcggat
    22861 tgcaccgcac ggttgatgac ttcattgact ttggcaaggt cttcaggcag atgtcgctgg
    22921 aaatagggca gattctcgag catggcagac tctcaggttt acgtttccgt caaacggcag
    22981 ataccgcttg aaagctgatt caaatagcgg gcaattatat cagcaaagcc atcagggtac
    23041 atcttttcag acggcatatg attgcccgaa ccgcgcaaac tttgacaaaa caggcaaata
    23101 acaatacaat gcccaatttc gcacaatggt gtgcggatat taaccataac ccttatggag
    23161 ttgagtatgt acgcggtcgt aaaaaccggc ggcaaacagt ataaagtttc cgtcggcgaa
    23221 aaattgaaag tagaacagat accagcccaa ctcgacagcc aaatcgaact gaccgaagtt
    23281 ttgatgattg ctgacggcga atctgtaaaa gttggcgcac cctttatcga aggtgcaaaa
    23341 gtaacggcta aagtagtggc acacggtcgt ggcgaaaaag tccgcatctt caaaatgcgc
    23401 cgccgcaaac actaccaaaa acgccaaggc caccgccaaa atttcaccca aatcgaaatc
    23461 gtggcaatcg cctaacctta aaacttagga gcattacaaa tggcaagtaa aaaagcaggc
    23521 ggcagcaccc gcaacggtcg cgattcagaa gccaaacgct tgggcgttaa agcctacggc
    23581 aacgagctga ttcccgcagg ttccatcatc gtacgccaac gcggtaccaa attccacgca
    23641 ggcgacaacg taggtatggg caaagaccac actttgttcg ccaaaattga cggttatgtc
    23701 gaattcaaaa ccaaaggcgc gctgaaccgt aaaactgtca gcatccgtcc ttacaccggt
    23761 tctgaagaat aattcaaccg atacctgaag ccgcatcttt tcacgatgcg gctttttaca
    23821 taccccatac ccgatttgat acacattaag ctgaaagtaa aaatccgcat acaccctccc
    23881 ctgcatattt cttcaacaac gggttttgat ataatcgcct atctgttaca gatagttcaa
    23941 acggcattca aacccttaca aatgccgtct gaaatcatcg gcatccgcct atgcaaagga
    24001 tattttatga gtttacacag cgacatcctc gtcgtcggcg caggcccggc gggtttaagt
    24061 tttgcagccg aacttgccgg cagcggtttg aaagttactc tgatcgaacg cagcccactg
    24121 actgttctgc aaaatcctcc ctatgacgga cgcgaaatcg ccctgaccca tttttcccgc
    24181 gaaatcatgc agcgtttggg tatgtgggac aaaattcccg aaaacgaaat ctaccccttg
    24241 cgcgatgcca aagtgctgaa cggacgttcc gactaccagc tccactttcc ccaaccgact
    24301 gaagcgcgcg gcgagcctgc cgactgtttg ggctatctga tttccaacca caatatccgc
    24361 cgcgccgcct atgaagtcgt atcacaactc gacaatgtca gcattctgac cgatacggcc
    24421 gttaaagaag tcaaaacatc tgataatgaa gcgcaagtct ttttggaaaa tggagacatt
    24481 ctaaccgcgc gtctcctttt ggcagcagac agccgtttct cacaaacccg ccgacaactc
    24541 ggtatttctt cagatatgca cgactacagc cgaaccatgt tcgtctgccg catgaaacat
    24601 accctttcca accaacatac cgcttacgaa tgcttccatt acggacgcac catcgcactg
    24661 ctgccactgg aaaagcgcct gaccaatacc gtcattacag tcgataccga taaaatcaac
    24721 agcgttcaaa acctttcgcc cgaagaattg gcggcaagcg taaaagaaca gctcaagggc
    24781 aggctcggcg acatggaact ggtcagcagc atccaccatt acccccttgt cggcatgatt
    24841 gccaaacgtt tctacggcaa acgcagcgcg ctgatcggcg atgcggctgt cggtatgcac
    24901 cccgtaacgg cacacggctt caacctcggg ctttccagcg cggatattct ggcaaaactg
    24961 atactcgaag ccgaacagcg cggtcaagac atcggcgcgg cttccttgtt ggaaaaatac
    25021 agcagcaaac atatgctgca cgcccatccg ctttatcacg gcaccaatat gatgctgaaa
    25081 ctgtttacca acgaaaccgc gccggcaaaa ctgttgcgcg gtttggtatt acgagccggc
    25141 aacaacttcc cgccgctgaa aaagctgatt accaaacaac tgaccggttg aaaactgccg
    25201 aaatgccgtc tgaaacgttt cagacggcat ttttgtatta tgaaccgttt tgcggtttcc
    25261 atatccttgg cagacggttt gcagcagatt cgcatacggc agatgtttca agcagaaagg
    25321 aaacacaata aaaataaaaa agcctccgaa cagtcggagg ctttgctttc aaactgaaat
    25381 tatttcagtt tggtttcttt gtacactacg tgtttgcggg caaccggatc gaattttttg
    25441 atttccagtt tgccgggcat agtgcgttta tttttggtag tggtgtagaa gtggccagta
    25501 cctgcaccgg attccagttt gattttatcg cgcattgcag taatccttaa ttaaatagtg
    25561 tttaaattaa gcttcgccgc gagcacgcaa atcagccaat acgacatcaa tgcctacttt
    25621 gtcgatggta cgcaatgcag cgttggaaac gcgcaggcga acccagcggt tttcactttc
    25681 tacccaaaaa cgacgtgatt gcaagttggg caaaaaacgg cgtttggttt tgttgttggc
    25741 gtgcgatacg ttgttgccgg acatcgggcg tttaccggtc actttgcaaa ctcgtgccat
    25801 aatcagtctc caaacttcta aaatagtaaa acgcgattta taccacgaaa aacaatgccg
    25861 tttcaagtgt tttaaagaaa aacagatgtt ttgcgcgggt aagccgcccg agtgccgcat
    25921 ctgcggaacg tttgcaggtg cgaaacagat ggggcgcgat tatagcggat ttcgggagct
    25981 tctgcattca aaatgccgtc cgaacatatt tccagtattt tcagacggca tttcaagttt
    26041 tcaaatccct caatgtccac cgccgccgct gtggaacggc ggtttcgcca gccagacaat
    26101 gggaatcagg acgataaaca aaatgctgcc ggcgaggaat atttcgttcg atccgatgat
    26161 gaagccctgt tgcgtaatgg tattgttgat gatgcctaga gtttgaccgt cggaaatgcc
    26221 ttgttgggac aaatgcgccg ccgtttcgtg caatgttgcg gaatagggcg tgatgtgttc
    26281 ggcaaaacgc gtgtggtgca acgcttcgcg ccgttcccac agggtgctga cgacggatac
    26341 gccgacaccg cccatcagca cgcgcaggaa attcgacagg ctgccggcgg cggcaatctg
    26401 tccgcccttc atgtgcgaca gtgtgatggt ggtcagcggc aggaagaaca tggcaacgcc
    26461 taccccctgc caaaactgcg gccagatgac gttgccgata tccatgtcgg cataaaaatc
    26521 agtgcgccaa taaaaagtga aggcaaaggt caggaagctg gcggttacga gcaggcgcat
    26581 atcgatttta ttgccgaacc tgccgattaa cggagatagg aagacgggga ggatgccgac
    26641 gggcgcggca gcaagccctg cccacgcgga ggtatagccc aagttggtct gcaacactaa
    26701 cggcagcagg gtcagcgtcc ccatatacac catgaaaccc aacgaggtgg caattacgcc
    26761 gacggtaaaa ttccgatctt taaacagcga taaatcgaca atcggatatt tttctcccaa
    26821 ttcccaaaca ataaaatacg acaagcacac cagtgcggtt atgcccaagg taatgatttc
    26881 tccagaggcg aaccagtcaa gttccttacc cctgtccagc atcatctgta acgcgccgat
    26941 accgaccatc atcagcgtca gcccgacata gtcggtcggc gttctgacgg ttgccgtttc
    27001 gcggtgtttc aaatgtttcc atgtaatcca tgccgatatg atcccgatgg ggatattaat
    27061 gaagaaaatc caaccccaat gccagtttcc ggaaatccaa ccgccgagta tcggcccgag
    27121 gacaggggcg acaacgacgg tcattgccca caatgccagt gccagcatcc gttttgcggg
    27181 cggataggat gccattaaca ggctttgcga caggggaatc aacggcccgg cgataaagcc
    27241 ctgcaaaatg cggaaaacaa ccagcgactg aaggttgggc gcgatgccgc acagccacga
    27301 tgcgatgacg aaaccggcgg cggcggcggt aaacaatttg acctcgccga tacgttttgc
    27361 caaaaagccc gtcagcggca cggaaacggc gtttgccacg gaaaaagaag tgatgaccca
    27421 cgtcccctgc gtggttgccg caccgaggtt gccggcaatg acggggacgg cgacgttggc
    27481 gatggtcgta tctaaaacct ccataaatac ggcaagcccc aaagacagcg taacccacgc
    27541 caatgccgca cccttaagcg gtggataatc cattgtgtcc tccgtttcag acggcatcag
    27601 ggcgcggatt gcccgaggat ttcatcgacc gtccgatcga cttccgacca gtcggtactt
    27661 tccatttccg gcaatgccgc acccggcgtt tttgaaacag gcgcgcctgc ggcggaagta
    27721 tccactttaa ccgtcatcga caaaccgata cgcaacggat gcctgtccac atcttcgcgg
    27781 ttcagcacga tacggacggg gacgcgctgc accactttaa tccagttgcc cgttgcgttt
    27841 tgcgccggaa tcagcgaaaa cgcgctgccc gtacctgccg aaaaccctgc caccctgccg
    27901 cgataaacaa tttgtttgcc gtacaaatcg gacaccagct cggcaggctg tccgattttc
    27961 atatgccgca actgcgtctc tttaaaattg gcatccaccc acacatccga cagcggcacc
    28021 accgccatca gcggcgcgcc tgccgccacc tgctgcccga cctgcaccga acgcttcgcc
    28081 acctgaccgt ccgccggcgc gcggacttgc gtccgccgaa ggttcagcca cgcatctttc
    28141 aacctgccga ttgccgtctg aacctccggc tgttcgcgca aagaaacatc tccgcccaaa
    28201 gccgcgcgtg ccgaagattc ttccgccaaa gccgccttaa ccgccgcctg cgcctgagac
    28261 actgccgtac gggcgtgtgc cagctcttcg gcggacaccg cacccgattc cgccaaagcg
    28321 gaccggcggc gcaaatcatc ctgcgcgcgc gccaaatccg cacggcgcaa ggcaacctgc
    28381 gcccccgcct gcgaagtggc ggcattttgc cggcggtttt ggcgcaccgc ctgaaccaac
    28441 tcgttttttg cccgctcgta agccagcaca tcattatcgt catccaacac cgccagcacg
    28501 tcgccttttt tcacggcatc cgtatcgtcg tgcaaaacct tccgcaccgt gccgcccttt
    28561 tgcggcgtaa cctgaaccac gcgtccggca acataagcgt cttccgtttc ctcttcgtgc
    28621 tgccaccata aaaaaaacgc cgatccggcg gctgcggcgg aaagcgcgaa cagcagcgtc
    28681 aatgccgtca ggcggcgttt gcgtttgact tgcgtgtttt gaagttttgt ttcgtctgtg
    28741 tgcgtttcca taagattggg ttccgaattt cctgatttca tataaacaat gttctttcaa
    28801 taggaaatag attttatatc cgcgcaataa ttcaatcaag tcagtatttt agatgcaata
    28861 ttcaaaaaac aacgcttcag gcggcaaaac tgaaaatata tttcgtttat atatgacatg
    28921 gcaaacaccg ccgcccgcac catgccgtct gaagaaaact acacaaatac cgccgcttat
    28981 attacaatcg ccgccccgtg gttcgaaaac ctcccacact aaaaaactaa ggaaacccta
    29041 tgtcccgcaa caacgaagag ctgcaaggca tctctctttt gggtaatcaa aaaacccaat
    29101 atccgaccgg ctacgcgcct gaaattctcg aagcattcga caacaaacat cccgacaacg
    29161 actatttcgt caaattcgtc tgccccgaat tcaccagcct ctgcccgatg accgggcagc
    29221 ccgacttcgc caccatcgtc atccgctata tcccgcacat caaaatggtg gaaagcaaat
    29281 ccctgaaact ctacctcttc agcttccgca accacggcga ttttcatgaa gactgcgtca
    29341 acatcatcat gaaagacctc atcgccctga tggatccgaa atacatcgaa gtgttcggcg
    29401 agttcacacc gcgcggcggc atcgccgttc atccgtttgc caactacggc aaagcaggca
    29461 cagagtttga agcattggca cgcaaacgcc tgttcgagca cgacgcacaa taaaccatcc
    29521 ttcccatatt tcagacggca ttccttcgat acggtatgcc gtctgaacgc ctttccgaaa
    29581 ggtttttatg tacgcattga ccgccgcaca gcaacagaag gcactcttcc ggctggtgct
    29641 tttccatatc ctcatcatcg ccgccagcaa ctatctggtg cagttcccct tccggatttt
    29701 cggcatccac accacttggg gcgcgttttc ctttcccttc atcttcctcg ccaccgacct
    29761 gaccgtccgc attttcggtt cgcacttggc gcggcggatt atcttttggg tgatgttccc
    29821 cgcccttttg ctttcctacg tcttttccgt tttgttccac aacggcagtt ggacgggctt
    29881 gggcgcgctg tcccaattca acacctttgt cggacgcatc gcgctggcaa gttttgccgc
    29941 ctacgcgctc ggacaaatcc ttgatatttt cgtattcgac aaattacgcc gtctgaaagc
    30001 gtggtggatt gccccggccg catcaaccgt catcggcaat gcactggaca cgttagtatt
    30061 ttttgccgtt gccttttacg caagcagcga tgaatttatg gcggcaaact ggcagggcat
    30121 cgcttttgtc gattacctgt tcaaacttac cgtctgcacc ctcttcttcc tgcccgccta
    30181 cggcgtgata ctgaatctgc tgacgaaaaa actgacggcc ctgcaaacca aacaggcgca
    30241 agaccgcccc gtgccctcgc tgcaaaatcc gtaaaacagc tctgtaggga atcgggacgg
    30301 ttgggttttc ttttatttgc aaacactgcc ggaacggata aatccggatt cccacccact
    30361 tcgtcattcc cgcgaaagcg ggaatccggt gcgttggggt tcagtcattt ccgataaact
    30421 cctgccgcat tgtatttcta gattcccgct ttcgcgggaa tgacggcgga tgggtttccg
    30481 tgccgtggtg tccggattcc cgccggtacg ggaatgacgg cagcgcttcc ttggcttagt
    30541 tttattttaa gccactataa atgccgtctg aaaacctgtt tgcagatttt cagacggcat
    30601 tgttttcgtc gtcggttcaa acttcaatcc gattgcgaca cggtaaccgg tatgccgcgc
    30661 aagcgcgcaa gcagcgcgtc cgccttctgt ttctgcgcgg caaacgccgc cttgtccgcc
    30721 tgcgtcaatt cgggtgtcgg caatgcgacc gaaacaggat tgacgggctg cccgttgatg
    30781 cgcgcctcgt aatgcaggtg cggcccggtc gaacgccctg tcgaaccgac aaaaccgatg
    30841 acctcgccgc cgcgcacatt gccttgtgcc tgcgaaaacg cgctcaagtg cgcgtacagc
    30901 gtttccacac cgttggagtg gcgtatcatc accgcgttgc cgtatccgcc cttccggcct
    30961 ttaaaggtaa tcacgccgtc ggcggaagcc ctgaccggcg ttccctgcgg tgcggcataa
    31021 tcgatgcccg tgtgcagccg ccatgtgtgc aggatggggt gcatacggta gccgaacggc
    31081 gaagaaatgc gcgtatagac cagcggctcg atgttgaagc cgcctttttc ctgcaacacc
    31141 ctgccgtctt catcgtaata attgccgccc cctccgcctt ccttgtccga acggtaatag
    31201 aacgcctgat gggttgtgtc gcccttgaca acttccgccg ccaaaatatc gcccgccgcc
    31261 acctgctgcc cgtggaaata caggctgtcg taaagcaggc gcacggcatc gccttccttc
    31321 aaaccgtcaa ggctgaagcg gccggcaaaa atcccgctta aggattcgcg gatttcgacg
    31381 ggcacttccg cccgcgccag cgaaccgcgc gccgacgttt tgaccacgac cgaacgcagt
    31441 gtcggcaaaa ccttcatatc cgcatcagaa gccgaccgcc gccatatgcc gccttttttt
    31501 tccaaagcga ccagattgcg ctcgccgtct tcgtcggtaa aaaactgcac ttcgcgcgca
    31561 ctgccgtcgc cgccgaccaa aacatgaacc gactggtcgg cacgcaaatg ccgcaaatcg
    31621 gcttcgccgc catatttttc cgtgattcgg gcaatctcgt cccgcgccat acccgaacgc
    31681 gccagcacgt ccgccagcga gtcgcccggc tgcaccgcct cctgcaccca atatgccgtc
    31741 tgaacgccgc tgccgcccca agacagcggc ggcagttttt gttccacgcg ctgcggtctg
    31801 acgcgctccg tcccctctgt cgaagcaatg tatgccgccg acaccagaat aatcgaaacg
    31861 gcaagcgcgc gcagcgcgta tttccgatgt tttgccgaaa gtgggaagac agccatattg
    31921 ttcctttttg acggcgtttg cgccgaatcc tctataatat ccgcccgatc ctcatttgtc
    31981 aggcatcaaa cgatgaaact caccctcgcc ctcccctcgc tcaatttgga tgaagacgaa
    32041 atccgaatac ctctatgcct gcccgccttc aacaaaatcc tgcaatacgg ctcgccgcac
    32101 cgccaaagct gcacggcttc ggcattttac gcacgttatt tgtggtgcgg aagactggca
    32161 caacgcccgg cgcaaagcct gaatatgccg tctgaaaccg tcgcgctcgc cacgcccgtc
    32221 tggcaaaaaa tggggctgca tcaggcaaat gtgctgacgg cggaatattt ggatgtcggt
    32281 acggatgaag cggaacggct ctgccgcgat ctgtcggcgt tttacggcga cattccgtgg
    32341 cgtttcgttc ccgtcctgcc cgaattatgg ctggtttccc ttccccgcgc gtaccgctgg
    32401 ggcgcgaagc ccgttttgga cttgggcgga ctgttgggcg cggacgatca gccggacggg
    32461 gaagacgcgt tggaatggct gcgcgtccaa accgaaatcc aaatgtggct gaacgctcac
    32521 cccgtcaacc acaaccgcaa aaaacgcggg ctgcccgaat tgaacggctt atggctttgg
    32581 gacagtctgc acggaagcgc acaaggcggc acgcttttcg ccgatacggt ttggagccgt
    32641 tttcacccga accgccgagc attgcccgac agcttccgag cttatgcgga aacggcggca
    32701 cacctgcccg atacgcacca catcctgttt atggacgatt tgcgcctgac cgccctgaca
    32761 ggggacaggg agcggtatgc ggcaatattg cagcagtggg aagaacgctg gttcgcaccg
    32821 ctttacgaag ccgtccgcac cggcaaaatc aaacggctgg acatcgccac ggacggacaa
    32881 cacggcggta cgctgacctt caaacccaca gacaggcgga agttttggcg ctgcacgaaa
    32941 acctttgacg gaatctggta aacccgcccg ccgcaaccgc gacaatgccg tctgaagccc
    33001 gaatcgggtt tcagacggca ttttaccccg catcgccgtt atgccggaaa aaccggaata
    33061 cgtttccgca cacgcggcgg cacgcaaaac accgcccgaa agccgccgat gcccgccgtg
    33121 acaccgccgc cggcagggtt tccgccgaaa aggaacagca atcagaacgt tttgttaaac
    33181 tcgacaaacg ccctgttttt ctcgtattcg ttaaacacat cgttgctcca cgtttcgcgg
    33241 tgcgacagcg tcaggcgcgg cgtgatgcct ttgaaatgca atgcccggtg ccaaaggctc
    33301 aaggatgtgt ccgattcttt atccctgcgc ctttcccctt taaaactgct gaagaagccg
    33361 ggtttttcat aatgccgttt cgccacgccg aggcggaaca gcgaagacag gccgctgccg
    33421 ccccattcct gcccccaggc aaagcgcagg ccgtaacggt tgaaattgtc gccacggtcg
    33481 gcggggttgc gctcgcggta aaaatccaaa ccgcccgtcc aatattggcg cgcattccgg
    33541 taaaacacca gcgaattgga aatttgcaaa tgggtattgt cggaacgcgc ccggcgcgta
    33601 ttcttcaaac gcccccactc cgccgaagac agcgtttgcc atctcggggt ttgccaacgg
    33661 ttgaaataaa ggcgtgcgcc gttggcgtaa gaataagcgt cgttgccgta ggtgcggcgt
    33721 tcgtggaaca ctgccagccc gacatcttta cgccggtcgg caaaaccgat gccgccggaa
    33781 acacctgccg tcatatcgtt gaatttctta ttccccggat aagccctgcc ggacacgtcg
    33841 ccgcccgccg tcgtgtacca gccgtttttc agcgaccatt ttttctccgc gccgaaccgg
    33901 taattgaccg ccgtgccgtc cacctgtttc gggaaagtcc agttgccgta ctgctgctgt
    33961 ttcggggctt ggttgatatt gtgttcgcgg gtaatgctga aaccgccgtt taccttccac
    34021 gcatcgcgtt cgcgcaatgc cttgcggtac agctcgacct gctccataag ctgcggcggc
    34081 agatcttctg ttttcaggcg gtcgaactgg tctgccgccg cctcgttctg cctgtcttca
    34141 aacaatgccg ccgccaaacg catacggacg gcgggcgcgt cgggttgggc ggcaatcaat
    34201 tcccggtaat gggaaaccgc ctccttcacc ctgccctctg cctgcgccaa aatcccttgt
    34261 gcataaagtg ccaacatctt atcctgccgc gcctgttgta ggtaaatcgg caaaatcacg
    34321 cggataccgg caatattgtt tgagaccact gcggaataca tggcacgcga caacaattcg
    34381 ggatttttca gcagggtttc gccgtcaacc tgcaaaactt ttcctttttc ccgcacctgc
    34441 cccggtacct tctccctgtc gatcggtttg acctccgctt catgaagcct gaactcggga
    34501 cggcttctca aatccggttc gcgcggtgtt tcttcggcgt atgccgcgct tgccaataaa
    34561 ggcagcagca tccatctgtt tcgtgcaggc ataaatgtct tcccacaaaa ataaaaataa
    34621 aaaataacca ttatcaaccg gcggattgta tcagtttttt atgccgctgt tccaaaccct
    34681 gccgcccgat gccgcgacaa tgccgtctga agccagaatc gggtttcaga cggcggcaaa
    34741 aaaccggcgg ttttccgccg gtttcatcat tatgccccgc ccttcggggc ggcagggttc
    34801 ggatcggttt gcggggcggc gcacggggcc gtgcaggtgt gccgcctgtg tgtcaaaaca
    34861 tttcccggcg catcaaaaca accatttgac cgacaatgcg gcttctttgt cgccgtccgt
    34921 ccttttgccg tatccgatgc gtgcggacat atggcctttc caaccggctt caatgccaaa
    34981 ccgcccttcg agcgccgtcc tgcctgccag cgtctgtttt tcgccgtcca tttccatgcc
    35041 gaaagatttt gacctgtgca aaacattaaa agcggcaaaa ggctgaagat tgacaccgtt
    35101 acgcaaagca aaacgggttt ttgcccgaat gccggcgcgg atttgccact gaccgctgcc
    35161 gagcagcccg accgccgccc ctcgctgtcg gtaaagccgc cgtttacgcc caagtaggta
    35221 aactgcgcct gcggttgcag gtaaaactgc atattactgc cttatatatt gcgtccctaa
    35281 gaaggggcga ttaacaaaaa ttaacgcccc ttcttaggga cgcaatatat aaggagtgag
    35341 tacctgttct tctattaaag aattaatctt ttgaggcatt ttctgttgaa gtttattaaa
    35401 cacatcttca gaaattccaa actgctttat tatctcttcc ctaacccgtt ttttattttc
    35461 ttcccaagcc tcgggtcttg ttttgtataa atcctgtaat tgctttttaa ctgcctgctt
    35521 taaatcagtt cggtaattat ctgtaatata tttcttaaca acaggcatat tatcgacggc
    35581 gaggatgccg ctattgtagt ttttacccgt aatattgatt tcgtcataca aggcttcaga
    35641 tgatttttga tcggttttac cgctggagaa ataatggaaa acaccgtttg ccggcttgtc
    35701 tgaaaatgaa aaatcggcag tatcgccaac atttgtagga cgagtatccg aaccgggctg
    35761 ataactgaaa gcaccgaagc aatcaaaaga cacactgcac gggccgcgcc aaacgtggga
    35821 atttctgtct tccgccgctt ctaaaataaa tttgttttta atctcggctt ctttgacggg
    35881 atcgtacgca tccgaacccc aagcatatgg cgtgcaaaac agcgataaaa cagataatgt
    35941 taatagatta acccgaccgc ttgcttgctt tcagtgtcat gagaaatttt cctttgtcaa
    36001 gtgttaaagt tataatgatt atatactata tcacactaca tcgcaatgaa aaatcgggaa
    36061 aaacaaaaaa cccctccgcc gtcattcccg cgaaagcggg aatctagaat tttaatgcgg
    36121 caagaattta tcggggatgg ccggggttta aaggtctggg ttcccgcctg cgcgggaatg
    36181 acggtttaga aatcacccga aacgagaaaa agaaccgaaa ccggacaagt cggattcccg
    36241 cgcgggcggg aatgacgata agcgtaattt caatccgcca tattgtcttt tcacttgcct
    36301 gaaaacagat ttcagccgcc tatccctgca tccggcggct gatgtagtcg gcaatcaaat
    36361 cccgcttttc ccaaaaaaac ttctgcattt tttgttcgga atacagtttg cggaccagag
    36421 tgggcagacc gctgtggacg atggcggcga tttctttgtt gccttcttct tcctgcattg
    36481 ccgtctgaaa atccaaaccc ctgcctttca gatatgctgc aaggcggttg cggatgtgtg
    36541 aattgagttc gatggatttg agcattttgc ctccgttttg cggtaaggat ttattcgatt
    36601 tgtttatccg cctgatttta tcacgtttcc caagcggttt gcctgtacaa taccgctttt
    36661 cggaagggtg tgggcgatga tgttttcttg gttcaagctg tttcacttgt tttttgtcat
    36721 ttcgtggttt gcagggctgt tttacctgcc gaggattttc gtcaatatgg cgatgattga
    36781 tgcgccgcgc ggcaatcccg agtatgtgcg cctgtcgggg atggcggtgc ggttgtaccg
    36841 ttttatgtcg cctttgggtt tcggcgcggt cgtgctcggc gcggcgatac cgtttgccgc
    36901 cggccggtgg ggcagcggct gggttcacgt caaactgtgt ttgggcttga tgctcttggc
    36961 ttatcagttg tattgcggcg tgctgctgcg ccgttttcag gattacagca atgctttttc
    37021 acaccgctgg taccgcgtgt tcaacgaaat ccccgtgctg ctgatggttg ccgcgctgta
    37081 tctggtcgtg ttcaaaccgt tttgacggag catcttatgc cgatagaaat cgaacgccgt
    37141 tttttgattg aaaacgacaa atggcggcaa tacgccgatg agccgctgct gctgaaacag
    37201 ggctacctgt ctgttgaaaa agaacgcacc atccgcatcc gcattgccgg aaaacgggcg
    37261 tggctgacgc tgaaaggcta tatttcggaa atcagccgca gcgagttcga atacgaaatc
    37321 ccgcttgccg atgcggaaaa gatgatggaa acgatgtgcc cgtttaagat ggaaaaacgg
    37381 cgttatccgg tcagatgggg cggcagcctg tttgaagtcg atgttttcct tggcgaaaac
    37441 tcgcctttgg tcgtcgcgga aatcgagttg cccgccgaaa atgccgattt tgaccgtccg
    37501 gactggctgg ggcgcgaaat cacttcagac ggtatgttta ccaatgccta tttgagcaaa
    37561 catccgttct caagctggaa aaatgccgtc tgaagcggct tcagacggca tctgccttgc
    37621 ggttatccct tgaggtaatg cacgaatgta tatgcaacgc ctttgctgct gacacggcgt
    37681 tccgtccgct ctgcttcttt ccaatgcgtc cggtctattt ctgggaaaaa tgcatctcct
    37741 tccacagaca aatccacttc ggttatccgc aaatcggtcg ctaatggcat cgcttgtccg
    37801 tatatctgcg cgccgcccat aatgacggct tcttccgcgc cggcgcacaa tgccaatgcc
    37861 gcctccaaac ttgccaccgt ttccgcgcct gccgcgcaat aatccgcctg ccggctgatg
    37921 acgatgttcc tccgtccggg caggggtttg acgggcaggg attcccacgt tttccgcccc
    37981 ataatgacgg gtttgcccaa ggtatagact ttgaaaaatg cgaaatcttc ggggatgtgc
    38041 caaggcatag cattgcccgc cccgatgcac aggttttccg cacacgccgc aattatggtt
    38101 attttgagca tattttctcc acaaaaatgc cgtctgaacg cttcagacgg cattttatga
    38161 cattctgcgt caaaaatctc aacctgcgcg cgccatacgt tttgtgttcg cacccgccaa
    38221 caatgccaac aattcttcag tcgcgcccca accgatacac gcatcggtaa tgctcttgcc
    38281 gtacacttcc ggcttgtcct gcctgccttc gaccaaatgg ctttccacca tcacgcccat
    38341 gatattgccg ccgtcctgtt ccaattggtc ggcaatgtct tgtgccactt ccatctgccg
    38401 ggtgtaatcc ttgcggctgt tggcgtggct gcaatcgatc atcagcttgt cggttacccc
    38461 tgccgcacgc agttgttcca ccgcctcgct gacgtgtccc gcatcataat tcggctcttt
    38521 tccgccgcgc aaaatgacat gacagtcggg attgccgccg gtatggacaa tggcggaatg
    38581 cccggccttg gttacagaca agaaatgatg cgaatggctc gccgcaccga ttgcgtcgat
    38641 cgcaatcttc aaattgccgt ccgtaccgtt tttaaagccg acggggcagg acagaccgct
    38701 tgccaattcg cggtggactt ggctttcggt cgtccgcgcg ccgattgccc cccaagaaat
    38761 caggtcggca taatactggg gtgtaatcat atccaaaaat tcggtagagg caggcatgcc
    38821 catattattc agggacaaca acaggctgcg cgcctgacgc aaaccgaaat tgatgtcgaa
    38881 cgtaccgtcc aaatgcgggt cgttaatcaa acctttccaa cccaccgtcg ttctcggctt
    38941 ctcgaaataa acgcgcatca cgatcaaaag ctcgttttca taccgcttgc ggagtttcaa
    39001 cagacgttcc gcatattcca acgccgcttt cggatcgtga atcgaacacg gcccgataat
    39061 gaccaacagc cgcctgtccc tgccgtgaac caaatcggaa atttcctgac gggtgcggtg
    39121 aaccaagccc gaagcctctt tggaaatcgg cagctcgtaa agatgggcaa tcggcggcaa
    39181 caactcttta acttctttaa tcttaatatc gtctgtgggg taatggtgtg tcatcttgca
    39241 tctttcctga tgggttttta tacgcggctt aggttacatc gtttaccttg cacaaacaag
    39301 tgcaacatat tactacaaag ctattttatt tcaaaataac tgatatatct tttttcatag
    39361 attttaaatt tcatattttt aatattttag gaattttatt tcaaagaaaa agaaaacaga
    39421 ttgataaaaa caatacccgc cattcattcg ggcaaaaccg cacggaacat ccgttttccc
    39481 gtttccacga caatccggta caggacaccc cgctgccgat tccgcctgca catccggcaa
    39541 aatttcatac gcaaggaaaa tgttgaaaac aaaaagaact aattgcttga aaaaagattt
    39601 aattggaatg cgggtatttc ccacactttc tccatttatt tatcctcatt ataaaaacat
    39661 gaaaaaaaac ccctgcttgc actctcactt gcagccgtgt ccgccgttac ggtagccgat
    39721 attcccgagt ctaacggcaa aggcgaatac aaagtcttta ccgcttatgt taaaggcaaa
    39781 gccactgccg gcaatttgaa agaaaaataa cgcacaccgt ccatcggttt tcgttcaacg
    39841 aacgccacga tatgcgtttt aaggcagagt acgcccgaag caaaaacgat gcagacgaca
    39901 aaagagggaa tgcacacatc gctaacggcg caataggcaa aatggctgcc gtgtatgccg
    39961 gttataccta cacccaaccg ggcggcgaat ccacacgttt ccgtggcggt ttggagcgtg
    40021 tccaagttaa acgttatctg ggagaaaaat gctgcggcta cgcctgtcca aaaagcgaac
    40081 gcttcaccct gcaagcagat ggtgaaatcc gtcaaaacct tggcgaaggc tggtttctga
    40141 agctatgagc ggggtggctg tctatctccg caacaaaaac aaaatagcaa ttgcctccca
    40201 agatgccaac ctaaactcca aaggccggtt cgtcagcagc ggtctgaatg tgggcaaaca
    40261 actgaccggc agtctcggcg tagagtttga cccatactac cgccaccgcg caatccgcaa
    40321 gtctgccgaa tttgtcccga acactaccaa aaccaaaaca gacagcgaaa agtttaacga
    40381 gtagggcttc cgcgtagccg caacgttcta attcccttac gtataaaaag cagcataaaa
    40441 atgctgcctt ttcccatttc caagggaagt attccgtagt gcagcttacc tcattgtttc
    40501 acacaaaccg aaaatgccgt cttaaaacca attttcagac ggcatttgtc ccttaaaaca
    40561 cgttttttca agcgcgacca taccaaacac ccacaggttg ccttccaccc ttcgtacaac
    40621 ctttgctcca tcaaacatcc acttcctaaa tactgatgta tactg
//
LOCUS       DAXDXW010000007        40217 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXDXW010000007 DAXDXW010000000
VERSION     DAXDXW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40217
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.007"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            196..1539
                     /locus_tag="ACFB64_000307"
     CDS             196..1539
                     /locus_tag="ACFB64_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC1208757.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYVGMRPSMK
                     IKEGEAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYVPEALAKLSSEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFVTGRLNTERVVALGGLQVNKPRLL
                     RTVLGAKVSQLTAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1542..2774
                     /locus_tag="ACFB64_000308"
     CDS             1542..2774
                     /locus_tag="ACFB64_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC1208758.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSPEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFASVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVANPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2767..3543
                     /locus_tag="ACFB64_000309"
     CDS             2767..3543
                     /locus_tag="ACFB64_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC1208759.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIRPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGVQ"
     gene            3543..4169
                     /locus_tag="ACFB64_000310"
     CDS             3543..4169
                     /locus_tag="ACFB64_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC1208760.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4173..4766
                     /gene="nqrE"
                     /locus_tag="ACFB64_000311"
     CDS             4173..4766
                     /gene="nqrE"
                     /locus_tag="ACFB64_000311"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC1208761.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYDFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4780..5997
                     /gene="nqrF"
                     /locus_tag="ACFB64_000312"
     CDS             4780..5997
                     /gene="nqrF"
                     /locus_tag="ACFB64_000312"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC1208762.1"
                     /translation="MEIILGIVMFTVIVLALALMILFAKSKLVSEGDITIKVNDEKEL
                     TMPAGGKLLGALASQGIFVPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLHSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6514..7569
                     /locus_tag="ACFB64_000313"
     CDS             6514..7569
                     /locus_tag="ACFB64_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC1208763.1"
                     /translation="MPSETRLPNLIRALIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAKIQKRIDDALKEVNRQMSTYQTDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILQQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNAHGEPWRIGIEQPNIIQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVSDSAMTADGLSTGLFVLGETEALRLAEQEKLAVFLIVRD
                     KDGYRTAMSSEFAKLLR"
     gene            7595..7810
                     /gene="nqrM"
                     /locus_tag="ACFB64_000314"
     CDS             7595..7810
                     /gene="nqrM"
                     /locus_tag="ACFB64_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC1208764.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRTIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7934..8512)
                     /gene="grpE"
                     /locus_tag="ACFB64_000315"
     CDS             complement(7934..8512)
                     /gene="grpE"
                     /locus_tag="ACFB64_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC1208765.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8695..9513
                     /gene="cysE"
                     /locus_tag="ACFB64_000316"
     CDS             8695..9513
                     /gene="cysE"
                     /locus_tag="ACFB64_000316"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC1208766.1"
                     /translation="MKKNHLNTTGFDLWHTIREETAAAAAAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQISKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARLGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFAEIDFMI"
     gene            complement(9717..11228)
                     /gene="ubiB"
                     /locus_tag="ACFB64_000317"
     CDS             complement(9717..11228)
                     /gene="ubiB"
                     /locus_tag="ACFB64_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC1208767.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCVSLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGKRLKPREVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HVESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKINALVDEARRQEMRDAYIHLVKVQQRQSLWLAAIAVVLLLILLLK"
     gene            complement(11263..11720)
                     /locus_tag="ACFB64_000318"
                     /pseudo
     CDS             complement(11263..11720)
                     /locus_tag="ACFB64_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(11717..11863)
                     /locus_tag="ACFB64_000319"
     CDS             complement(11717..11863)
                     /locus_tag="ACFB64_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208768.1"
                     /translation="MPSERSDGIFTFGKYIIGMNLHIIRLLEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(11880..12218)
                     /gene="erpA"
                     /locus_tag="ACFB64_000320"
     CDS             complement(11880..12218)
                     /gene="erpA"
                     /locus_tag="ACFB64_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC1208769.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(12351..13037)
                     /locus_tag="ACFB64_000321"
     CDS             complement(12351..13037)
                     /locus_tag="ACFB64_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1208770.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVGHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTV"
     gene            13224..13496
                     /locus_tag="ACFB64_000322"
     CDS             13224..13496
                     /locus_tag="ACFB64_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208771.1"
                     /translation="MKKTSKYLIYTAAFTSFCFAFQENRSEAKQPDITLSASLCEQFN
                     MLNAKDMDTEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGVVYE"
     gene            13704..15632
                     /gene="dnaK"
                     /locus_tag="ACFB64_000323"
     CDS             13704..15632
                     /gene="dnaK"
                     /locus_tag="ACFB64_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC1208772.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSIIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKADNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKIAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKTAIDAKAEALGTASQKLGEMVYAQAQAEAQAGEGAQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            15855..17781
                     /locus_tag="ACFB64_000324"
                     /pseudo
     CDS             15855..17781
                     /locus_tag="ACFB64_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            17838..18815
                     /locus_tag="ACFB64_000325"
     CDS             17838..18815
                     /locus_tag="ACFB64_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFC1208773.1"
                     /translation="MDMKRRDFLKMTAALAAAGVSPSLLTAGKEQFTVYGAPAMPSVT
                     IAVTALQGKLAKQADVSLKIWRSPDQLRAGVASGQFKVMMSPSNVGVNLRNQGQKVGM
                     VNILTNGITQLVCKGSAIASPQDLVGKKILVPFKNDMPDIVLQALLKKLKIDAHKVSI
                     TYAATPPEAVGLFPSKGYHAVILPEPMATASLLKGKTIGINVVHGFDLVKAWGQAFGT
                     KPLIPMAGIIANEEYFHAHKAQFDIFHQDLKNALNWILANRQNAAKIGKNYLPAPEPA
                     LVMGLDGARLTVSKGSEVKNEILKFYEILMQFNPELLGGKLPDNGFFLA"
     gene            19152..19289
                     /locus_tag="ACFB64_000326"
     CDS             19152..19289
                     /locus_tag="ACFB64_000326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208774.1"
                     /translation="MNEDKYRENMTWDEIENVVGTLVSEERMLKSNSFLSIKNSKNRL
                     A"
     gene            19368..20137
                     /locus_tag="ACFB64_000327"
                     /pseudo
     CDS             19368..20137
                     /locus_tag="ACFB64_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776012.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            20164..20856
                     /locus_tag="ACFB64_000328"
     CDS             20164..20856
                     /locus_tag="ACFB64_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC1208775.1"
                     /translation="MLCLENVRFEILRDPIVRDFSLNLQQGEVKALFGPSGCGKTTVL
                     RLIAGLETPKSGTIRNTFHKTGFLFQENRLPENLTAMQNIAIFMDNPDEGEIVALAAK
                     VGLTAGDLNKYPTELSGGMAKRVAFLRLLLCGCDLALLDEPFVGLDRDLRDILVAMLV
                     EKIERQGMACILVTHDRFEAARLSHEIMLLSAKGMNVQNVITLPTPLSERDSAFEEVV
                     VAREFQGIHYYE"
     gene            21048..21662
                     /locus_tag="ACFB64_000329"
     CDS             21048..21662
                     /locus_tag="ACFB64_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC1208776.1"
                     /translation="MFSTVITAAVLYIATAVDLLVILLIFFARANTRKEYRDIYIGQY
                     LGSVILILVSLFLAFVLNYVPEKWVLGLLGLIPIYLGIKVAIYDDCEGEKRAKKELDE
                     KGLSKLVGIVALVTVASCGADNIGLFVPYFVTLDLVDLLVTLLVFLILIFVLVYTAQR
                     LANISGVGEIVEKFSRWIMAVIYIGLGLFIIIENNTIRTIISII"
     gene            21958..23054
                     /locus_tag="ACFB64_000330"
                     /pseudo
     CDS             21958..23054
                     /locus_tag="ACFB64_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
     gene            complement(23101..25290)
                     /locus_tag="ACFB64_000331"
     CDS             complement(23101..25290)
                     /locus_tag="ACFB64_000331"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC1208777.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSEPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDAARILSVQTVFVEEKPLSQSWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQANAQA
                     AKLIEDWAEQGWIETTEAAKPVLRPYRGQASHSEFVLNTGQQKASDEIQTALGRFRSF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPLPDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHASAQLPQVDIL
                     NIGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRALLPKAAVV
                     RVDRDSTAHKNDWADLYRRIANDEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSRFGAAPMLMVRLA
                     ERERAQVFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            25430..26215
                     /locus_tag="ACFB64_000332"
     CDS             25430..26215
                     /locus_tag="ACFB64_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC1208778.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAESAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRTQSGYSPMPQLEEIIRKNQQ"
     gene            complement(26314..28248)
                     /locus_tag="ACFB64_000333"
     CDS             complement(26314..28248)
                     /locus_tag="ACFB64_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC1208779.1"
                     /translation="MSLIECKNINRCFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGMGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSMGTNTISIFPGRGFGDRRSGKIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTRVAEKGLAELLKARHGTEDFFMNNSDSIRQ
                     MVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICIIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(28314..29492)
                     /locus_tag="ACFB64_000334"
     CDS             complement(28314..29492)
                     /locus_tag="ACFB64_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC1208780.1"
                     /translation="MAKMMKWSAVAAVAAAAVWGGWHYLKPEPQASYITETVRRGGIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTTQTNTLDMEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESDLGYTRITATMDGTVVAIPVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLLIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            29718..30107
                     /locus_tag="ACFB64_000335"
     CDS             29718..30107
                     /locus_tag="ACFB64_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC1208781.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLIAYPSYKTYIRRARLSEVK
                     STLLVNAQNLERYYRQKGTFEKYDSTKLKQNKYFKITLIKASPDHFTLQAAPDTATNE
                     GETCTVTLNDGGTIAASGTNQSCPGFD"
     gene            complement(30201..33686)
                     /gene="smc"
                     /locus_tag="ACFB64_000336"
     CDS             complement(30201..33686)
                     /gene="smc"
                     /locus_tag="ACFB64_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC1208782.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNNEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIRQE
                     QQQIRVQLEENELQVEEKQTELAEWAMQVAEHEERLPELEEVQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARSVPNSFVPPAPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGTHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELENLAPGLSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRTAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIATLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERRQELALAYETEFQNDEQHIKLDELT
                     ETVHTLDEEYIAVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDVLEALAKESAKVLNSSIGSLTRQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAIALLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCKLVKEMSVQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            34063..34731
                     /locus_tag="ACFB64_000337"
     CDS             34063..34731
                     /locus_tag="ACFB64_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208783.1"
                     /translation="MGLRQKLRGIIPNLATSIGTSAEANAPGALALRGNSEASKKFSI
                     AEGYLASSDGYGAIAIGSAAKIKQLEKGTINHIVGNDNKGLYVDADGNVTKITVRTES
                     EKDILSRYGQTYGAMALGFRSSSHNLFASSFGAFSTATAIESLAVGDSSQSTGYRSAT
                     FGSHSRALAEESLALGYETRANAYGSVALGAESVANEENTVSVGSDTLKRKIVNVADG
                     TEDL"
     gene            34712..34900
                     /locus_tag="ACFB64_000338"
                     /pseudo
     CDS             34712..34900
                     /locus_tag="ACFB64_000338"
                     /inference="COORDINATES: protein motif:HMM:NF015831.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            34950..35183
                     /locus_tag="ACFB64_000339"
     CDS             34950..35183
                     /locus_tag="ACFB64_000339"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208784.1"
                     /translation="MPDRAFGAGVSWKFGGKPTPAVAAQNAAHPAKVLQLRQEVAALR
                     ARQAETDRKLHKQAEMENELQQLRRALSELKKH"
     gene            35392..36114
                     /gene="lpxH"
                     /locus_tag="ACFB64_000340"
     CDS             35392..36114
                     /gene="lpxH"
                     /locus_tag="ACFB64_000340"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC1208785.1"
                     /translation="MRPSYFISDLHLSEKHPELTELLLRFLRSAAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGRDFCRQAGMTLLPDY
                     SVLDLFGSNTLICHGDTLCTDDKAYLRFRRIVHCRRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPPEEC"
     gene            36383..37969
                     /locus_tag="ACFB64_000341"
     CDS             36383..37969
                     /locus_tag="ACFB64_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC1208786.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYAALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(38135..38338)
                     /locus_tag="ACFB64_000342"
     CDS             complement(38135..38338)
                     /locus_tag="ACFB64_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC1208787.1"
                     /translation="MDNLNPQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLGEGESG
                     SVACPYCGTRYRLDGKMPHHYYA"
     gene            38454..38951
                     /locus_tag="ACFB64_000343"
     CDS             38454..38951
                     /locus_tag="ACFB64_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC1208788.1"
                     /translation="MTALTLPEDIRQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHILSNRAAFA
                     HIGQA"
     gene            39033..>40217
                     /locus_tag="ACFB64_000344"
     CDS             39033..>40217
                     /locus_tag="ACFB64_000344"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC1208789.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKRLLESETTKNYLTIDGVADYNEQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHCLDW
                     DGMIEDLNQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     STQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDNNIDYLCES
                     IVK"
BASE COUNT         9621 a        11089 c        10116 g         9391 t
ORIGIN      
        1 tcaaatcaat atcccccttc cgacaattaa aaacggcttc cgcaaggaaa aaagcggcaa
       61 atctgacaaa caggtacaat tcctgataaa atctaacact ttgctacatt aatttattca
      121 atgcttttga tgccacgcga cacggtatca aaaacacgcc gtttttacct taaccatcac
      181 aagtaatgca aatctatgat taaaatcaaa aaaggtctaa atctgcccat cgcgggcaga
      241 ccggagcaag tcatttatga cggcccggcc attaccgaag tcgcgttgct tggcgaagaa
      301 tatgtcggca tgcgcccctc gatgaaaatc aaggaaggtg aagccgtcaa aaaaggccaa
      361 gtgctgtttg aagacaaaaa gaatccgggc gtagtattta ctgcgccggc ttcaggcaaa
      421 atcgccgcta ttcaccgtgg cgaaaagcgc gtacttcagt cagtcgtgat tgccgttgaa
      481 ggcaacgacg aaatcgagtt cgaacgctac gtacctgaag cgctggcaaa attgagcagc
      541 gaagaagtgc gccgcaacct gattcaatca ggcttatgga ctgcgcttcg cacccgtccg
      601 ttcagcaaaa tccctgccgt agatgccgag ccgttcgcca tcttcgtcaa tgcgatggac
      661 accaatccgc tggctgccga ccctacggtc atcatcaaag aagccgccga agacttcaaa
      721 cgcggcctgt tggtattgag ccgcctgacc gaacgtaaaa tccatgtgtg taaagcagca
      781 ggcgcagacg tgccgtctga aaatgctgcc aatatcgaaa cacatgaatt tggcggcccg
      841 catcctgccg gcttgagtgg cacgcacatt catttcatcg agccagtcgg cgcgaataaa
      901 accgtgtgga ccatcaatta tcaagacgtg attgccatcg gacgtttgtt cgtaacaggc
      961 cgtctgaata ccgagcgcgt ggttgccttg ggcggcctgc aagtcaacaa accgcgcctc
     1021 ttgcgtaccg ttttgggtgc gaaggtgtct caacttaccg ccggcgaatt ggttgacgcg
     1081 gacaaccgcg tgatttccgg ttcggtattg aacggtgcga ttgcacaagg cgcgcatgat
     1141 tatttgggac gctaccacaa tcagatttcc gttatcgaag aaggccgcag caaagagctg
     1201 ttcggctggg ttgcgccgca gccggacaaa tactccatca cgcgcaccac tctcggccat
     1261 ttcctaaaaa acaaactctt caagttcacg acagccgtca acggcggcga ccgcgccatg
     1321 gtaccgatcg gcacttatga gcgcgtaatg ccgttggaca tcctgcctac cttgcttttg
     1381 cgcgatttaa tcgtcggcga taccgacagc gcgcaggctt tgggttgctt ggaattggac
     1441 gaagaagacc tcgctttgtg cagcttcgtc tgcccgggca aatacgaata cggcccgctg
     1501 ttgcgcaaag tgctggaaac cattgagaag gaaggctgat tatgggctta aaacattttc
     1561 tggaaaaaat cgaaccgcac ttcctgccgg gcggcaaaca tgaaaaatgg tatgccctct
     1621 atgaagctgc ggcgacgatt ttctatacat ccggcgcggt aacgcgcaaa gcggcgcacg
     1681 tccgcgacgc gctcgactcc aaacgcatga tgattttggt gtggctggct ttgttccccg
     1741 ccatgtttta cggcatgtac aacgtcggcg cacaggcatt cggtgcctta acgcccgatt
     1801 tgctgcaaca aagcatcgcc aacgactggc attacgccct tgccaacgct ttgggcatca
     1861 atatgtcgcc tgaagcgggc gtgttgggca aaatgctgtt cggcgcgatt tacttcctgc
     1921 cgatttacgc gaccgtattt attgtgggcg gcttctggga agtcttgttc gcatccgtac
     1981 gcaaacacga aatcaacgaa ggtttcttcg ttacttcgat tctgtttgcc ttaatcgttc
     2041 cgcccacgct gccgctgtgg caggcggctt tgggtatttc tttcggcgtt gtggttgcga
     2101 aagaagtatt cggcggtaca ggtaaaaact tcatgaaccc tgcgctggca ggccgcgcct
     2161 tcctgttctt cgcctacccc gccaacttga gcggcgatgc ggtttggacg gcggttgacg
     2221 gctattccgg cgcaaccgcg ctggcgcaat gggcggcaca cggtgcagac ggcctgaaaa
     2281 acgccgtaac cggtcaaacc atcacttgga tggacgcatt tatcggcaaa ctgcccggct
     2341 ccatcggcga agtctccact ttggcactct taatcggcgg cgcgtttatc gtgtttgccc
     2401 gcatcgcttc ttggcgcatt attgccggcg tgatgatcgg tatgattgcg atgtcttcgc
     2461 tgtttaactt catcggttct gacaccaacg ctatgtttgc tatgccttgg tactggcact
     2521 tggtggtcgg cggcttcgcc atcggtatgc tgtttatggc gaccgacccc gtttccgctt
     2581 cctttaccaa tgtcggcaaa tggtggtacg gcgcgcttat cggcgtaatg tgcgtgttaa
     2641 tccgcgtggc caatccggct taccccgaag gcatgatgtt ggcgattctg tttgccaacc
     2701 tgtttgcccc gattttcgac tatttcgtcg cacaagcgaa catcaaacgc agaaaggcgc
     2761 gcagcaatgg ctaagaaatt cgataaagac agcttcagcg gcacgctgat tgtcgtgttg
     2821 gcggtcagcc tgatttgctc ggtcatcgtt gccggtgcgg tcgtcggctt gaaacccatc
     2881 caagaaaaac aaaaactcca agacaaacaa ggctatattt tgagcgtggc cggtttgatg
     2941 gataaggaca ccgacatcgg taaaaccttt gccgagcgta tcgagcaacg cgtggtcgat
     3001 ttggcgaccg gcgaatatgt gaaagacgcg ccgaaagact tcagcgcgcg catcgcaggc
     3061 aaagaccccg cgcaaagcat ccgcatcaaa cctgaagacg atttggcagg catcaaaagc
     3121 cgcgccaaat acaccgaggt ttatttggta aaaggcgaag acggcaaaat cgggcaaatc
     3181 atcctgccga tgcacggcaa cggtttgtgg tcggtgatgt acggcttcgt cgccatccga
     3241 cccgacggca acaccatcaa cggcattacc tactacgaac aaggcgaaac cccgggcttg
     3301 ggcggcgaaa tcggcaatcc tttgtggcag caaaaattcg tcggcaaaaa attgtttgac
     3361 ggacaaggca aactcgccct gcacgtgggc aaaggcgcgg gttcggacaa agaacacggc
     3421 gtagacgccc tctccggcgc gtcgctgact tccaaaggcg tgcaaggttc gttcgcctac
     3481 tggttcggcg aaaacggcta tatcccctac ctgaacaaat tgaaatcagc aggagtgcaa
     3541 taatggctga tatgaaacgc ttgaaacatt tgatgttctc accctttatc gacaacaacc
     3601 cgattgcctt gcaggttttg ggtatttgtt cggctttggc ggttaccacc aaacttcaga
     3661 cggccatcgt aatgggtatt tccgtagctt tggtaaccgg tttttccagc ttcttcattt
     3721 cgctggtgcg caactacatc cccaacagca tccgcatcat cgtgcaaatg gcgattatcg
     3781 cgtcgctggt tacgctggtc gaccaactgc ttcaggcatt tgcctacgaa ttgtccaagc
     3841 agctttccgt attcgtcggc ctgattatta ccaactgtat agtgatgggc cgtgccgaag
     3901 catttgcgat gaaagagccg ccgctggaaa gcctgatcga cggcatcggc aacggtgcgg
     3961 gctacgggat gatgctgctt gtcgtcgcca ccgtccgcga actgatcggc tcaggcaaac
     4021 ttttaggtta taccgtcttt caaaccgtac aggacggcgg ctggtatcag accaacggcc
     4081 tcttcctact cgcccccagc gcgttcttca tcatcggctt tttgatttgg ggactgcgta
     4141 cttggaaacc cgaacaggcg gaggaataag ctatggaaca ctatttgagc ctcttcatca
     4201 aatccgtctt cattgaaaat atggcgctat ccttcttttt gggtatgtgt acgtttttgg
     4261 cggtatccaa aaaagtatcc accgcattcg gtttgggtgt ggcggtaatt ttcgtactcg
     4321 ggctgtccgt ccccgccaac caattggttt actcgctgct caaagacggc gcgattgtcg
     4381 aaggcgtgga tttgaccttt ttgaaattca tcacctttat cggcgtgatt gccgctttgg
     4441 tgcagatttt ggaaatgttc ttggacaaat tcgtccccgc cctctataac gcgctgggca
     4501 tctacctgcc tctgattacc gtaaactgcg cgattttcgg cgccgtatcg tttatggcgc
     4561 aacgcgaata cgacttcggc gagtccgtcg tgtacggctt cggcgcgggt ttgggctgga
     4621 tgttggcgat tgtcgctttg gcgggcatta ccgaaaaaat gaaatattcg gacgctccca
     4681 aaggcctcaa aggcttgggc attaccttta tcgccgccgg cctgatggca atggcgttta
     4741 tgtcgttctc cggcatccag ttataagaaa gggaccggta tggagattat tttaggtatc
     4801 gtgatgttta ccgtcatcgt cttagctttg gcactgatga ttctgtttgc caaatccaag
     4861 ttggtcagcg aaggcgacat caccatcaaa gtcaatgatg aaaaagaact gactatgccc
     4921 gccggcggca aactcttggg cgcgcttgcc agccaaggca tctttgtccc ctccgcctgc
     4981 ggtggcggcg gttcgtgcgg acaatgccgc gttgtcgtga aaagcggcgg cggcgacatt
     5041 ctgccgaccg agctgtccca catcagcaaa cgcgaagcac gcgaaggctg ccgtttgtcc
     5101 tgccaagtca atgtcaaaac cgacatggac atcgaagtac ccgaagaagt gttcggcgtg
     5161 aaaaaatggg aatgcaccgt catttccaac gacaacaaag caacgttcat taaagaactc
     5221 aagcttgcca ttcccgaagg cgaagaagtt cctttccgcg ccggcggcta cattcaaatc
     5281 gaagccccgc cgcacaccgt tgcctacaaa gacttcgaca ttcccaagga atatcacgaa
     5341 gactgggaca aatacaatct gtggcaatac gtttccaaag tggacgagcc gattttgcgc
     5401 gcctactcta tggcttcgta tcctgaagaa aaaggcatca ttatgctgaa cgtgcgtatc
     5461 gccacgcctc ccccgcgcgt acccgatgcg cctccgggac aaatgtcgtc ctacatctgg
     5521 tcgctcaaac ccggcgacaa agttacgatt tccggcccgt tcggcgaatt tttcgccaaa
     5581 gacaccgatg ccgaaatggt atttatcggc ggcggtgcgg gtatggcccc gatgcgttcc
     5641 cacattttcg accagttgaa acgtttgcac tccaaacgca aaatcacctt ctggtacggc
     5701 gcacgctcca aacgcgaaat gttctatgtc gaagacttcg accaactcgc ggcagaattc
     5761 ccgaacttca cttggcacgt cgccctgtcc gacccattgc ctgaagacaa ctgggacggc
     5821 tacacgggct tcatccacaa cgtggtttac gaaaaccacc tgaaaaacca cgaagcaccg
     5881 gaagactgcg aattttatat gtgcggcccg ccgattatga accagtccgt catcaaaatg
     5941 ctcaaagact tgggcgtgga agacgaaaac atcctcttgg acgatttcgg cggttaaata
     6001 ttcggcttca aacacaaaag gaagaaaccc cggtttcttc cttttttatc cgatgccgtc
     6061 cgaacaccct tcagacggca tccgcccata cccgtatcaa tatcgttttt ttaccctgcg
     6121 gcggatgtgc ggctaaatat tttgacgaaa aaacaaagaa gtatacttct ttttagttat
     6181 ggttgattcc ataactaaaa aagtatcgat caacccattt tacgaggtac ggataatgtc
     6241 tgacagcttt gaaaacagcc ccgaatacga tgattggatt gaatcgggcg gtcgtgatga
     6301 agattacgaa tactactaca acaaatggca acgcaggacg cgctgaccat taaaccctga
     6361 caaaccggtt tgtaaaacac cggtttgttt tttattaccg ccacccaaac gacgggcgat
     6421 gccgcccgaa cgcccttcaa acggcatcgg catagaaaca taccggccgt tttgcgatat
     6481 aattcaagcc atttgccaaa accgtcaaac actatgccgt ctgaaacacg cctgccgaac
     6541 cttatccgcg ccttgatatt tgccctgggt ttcatcttcc tgaacgcctg ttcggaacaa
     6601 accgcgcaaa ccgttaccct gcaaggcgaa acgatgggta cgacctatac cgtcaaatac
     6661 ctttcaaata atcgggacaa actcccctcc cctgccaaaa tacaaaagcg cattgatgat
     6721 gcgcttaaag aagtcaaccg gcagatgtcc acctaccaga ccgattccga aatcagccgg
     6781 ttcaaccaac acacagccgg caagcccctc cgcatttcaa gcgatttcgc acacgttacc
     6841 gccgaagccg tccgcctgaa ccgcctgact cacggcgcac tggacgtaac cgtcggccct
     6901 ttggtcaacc tttgggggtt cggccccgac aaatccgtta cccgtgaacc gtcgccggaa
     6961 caaatcaaac aggcggcatc ttatacgggc atagacaaaa tcattttgca acaaggcaaa
     7021 gattacgctt ccttgagcaa aacccacccc aaagcctatt tggatttatc ttcgattgcc
     7081 aaaggcttcg gcgttgataa agttgcgggc gaactggaaa aatacggcat tcaaaattat
     7141 ctggtcgaaa tcggcggcga gttgcacggc aaaggcaaaa atgcgcacgg cgaaccgtgg
     7201 cgcatcggta tagagcaacc caatatcatc caaggcggca atacgcagat tatcgtcccg
     7261 ctgaacaacc gttcgcttgc cacttccggc gattaccgta ttttccacgt cgataaaaac
     7321 ggcaaacgcc tctcccacat catcaatccc aacaacaaac gacccatcag ccacaacctc
     7381 gcctccatca gcgtggtctc agacagtgca atgacggcgg acggtttatc cacaggatta
     7441 tttgttttag gcgaaaccga agccttaagg ctggcagaac aagaaaaact cgctgttttc
     7501 ctaattgtcc gggataagga cggctaccgc accgccatgt cttccgaatt tgccaagctg
     7561 ctccgctaaa catacaaacc gattaggaaa caccatgaaa accctgctcc tcaccttcgg
     7621 catcttcctg accgtcatca tcggtatggc ggtcggctat attttctcca aacgcaccat
     7681 caaaggcagt tgcggcggca ttaccgcttt aggtatgaaa aaaatgtgcg actgcgacac
     7741 accttgcgac accctgcaaa aaaagctgga tgaagaaaaa caggcaggcg gcatacgggt
     7801 tgaccgttaa cgccgaaaca aaatgccgcc tgaagcccgg ccggcgggtt cagacggcat
     7861 tgccgcttta cttcgatcgg tttatacgca aaacgcgctt caggaaataa atcagattat
     7921 tccccagacg ccttcaggct tccttctgtg ccaccgtaac catagccggg cgcaacacac
     7981 ggtcggacag ggtataaccc ttcttcatca caccaaccac ggtattgggt tcctgctcac
     8041 tggccaccgc ctgcatcgcc tgatggatat tcggatcgag cttatcgccc gctttaggat
     8101 tgatttcctt gatttgcgtg gcatcaaacg ccttctgcaa ctcattcaaa gtcatctgca
     8161 cgcccatttt cagcgcatcg aaattaccgc tctgatccaa aagcgccatt tccagataat
     8221 ccttgaccgg caacatttcc acggcaaact tctgtccggc gaacttgtgc gtatcggcaa
     8281 tttcctgctg gtggcggcgg cgcaggtttt gctcgtttgc caaagcgcgc agttgttcgt
     8341 ctttcaactg cgcttccaac tcggcaatcc gcgcctgcaa atcctcataa gccggctcgg
     8401 cggcagcctg ttcctgtaca ccgtccgcat ttcctactgt ctcgacggtt tccaccgcct
     8461 ccacattttc aaccgcttct tcactgtttt gctgctgtgt ctgttcgctc atatcgtatc
     8521 cctcaaaaca aattggaaat caaaatccgg ttattacccg agcaagataa ggacattttc
     8581 aagaaacttc aagcaaaggc aggaaatttc acatccgccg ccgaataccc taccgcaaaa
     8641 accatataaa cggtaagata ccgccgacct tatgcttttc agacggcatc cattatgaaa
     8701 aaaaaccatt tgaacacaac cggtttcgac ctctggcaca ccatccgcga agaaaccgcg
     8761 gccgctgccg ccgccgaacc gatgctggca agttttttgc accaaaccgt gttgcgccac
     8821 gagtccctcg gctccgtcct tgcctaccac ctttccagca aactcggcag cccgattatg
     8881 gacgtgcgcg cgctgtttga aatttaccag caggcattgg gcagcgacac ccaaatcagc
     8941 aaatgtgtcg aagcagactt aaaagccatc tacgaacgcg atcccgcctg cgacgaatat
     9001 tcgctgccgc ttttatattt caaaggcttc cacgcgattc aggcacaccg catcaaccac
     9061 cggctgtatc tcgacggacg caaaacgctg gcgtatttcc tacaaaaccg tatgtccgaa
     9121 gtattcggcg tggacatcca tcccgccgcc cgtttaggat acgggctgat gctcgaccac
     9181 gccaccggct ttgttgccgg agaaaccgcc gtgttgggca acaatatttc gattttgcac
     9241 ggcgtaacgc tcggcggttc gggcaaagaa ggcggcgacc gccaccccaa aatcggcgac
     9301 ggcgtgatga tcggcgcaaa cgcctcgata ttgggcaata tccgcatcgg cagcaatgcc
     9361 aaaatcggcg cgggcagcgt cgtggtttca gacgtgccgc cgtccatcac ggttgtcggc
     9421 gtacccgcca aacccgtggc gcgatcgctc aaaaccccgt cggcggatat ggatcaaaat
     9481 atccagtttg ccgaaatcga ctttatgatt tgaatatccg caccaatgcc gtctgaaacg
     9541 ccaaacgggc ttcagacggc attgcccgct tcaagcctgc gccttaatat gccgccgatg
     9601 ccggcactta tcagtcggac aaaatgcctt ttattcgata cgcccgattc ccacgcaacc
     9661 ggaaataacc ggtttccttg taaacgggcg gagtcgggca atgattcgga caaagcctat
     9721 ttcaaaagca aaatcagcag caaaacaacc gctatcgctg ccagccacag gctttgccgc
     9781 tgctgcactt tgaccaaatg gatataggca tcgcgcattt cctgccggcg cgcctcatcg
     9841 accaaggcat tgattttgcg cggcagggaa gggatgattt gcgcccagtc tggggcttcg
     9901 tttttgaggt tgcgccaaag ggctttggga ccgacctgcc cgttcatcca ttttaccaaa
     9961 aacggtttgg cggttttcca caagtccaaa tcgggatcga gttggcggcc caagccttcg
    10021 atgttgagca gcgttttttg cagcaatacc agctgcggct ggatttcgac attgaagcgg
    10081 cggctgactt caaacaggcg catcagtacc aagccgaagg aaatctgcga aatcggtttg
    10141 ttgaacacag gctcgcacac ggcgcgaacc gccgcttcca actcttccgc acgcgtgtcg
    10201 gcaggcaccc agcccgattc gacgtgggcg gtggcgacac gtcggtaatc gcggttgaaa
    10261 aaggcgagga aattgatggc gagatagcgt ttgtcgtaat cggtcagcgt accgacgatg
    10321 ccgaaatcaa gggcgatata gcggttgtcg gcggcaacca aaatattgcc ggggtgcata
    10381 tccgcgtgga aaaagccgtc gcggaaaact tgcgtgaaaa agatttccac gccgtaatcg
    10441 gcaagtttgt gcaaatcgat gccgtctgct ttgagtttgg cgatgtcgga aaccggcgtg
    10501 ccgtccatcc attcaatcgt cagcacgtcg cttgtgcagt agtcgtaaaa caccttgggc
    10561 acaatcagca tattgctgtt ttggaaattg cgcccgagtt gtcctgcatt ggcggcttcg
    10621 cgcatcaaat ccaattcgtc gtgcaggtat ttgtcgaact ccgccaccac ttcgcgcggc
    10681 ttcagacgct tgccgtctga aaacaagcgt tcgacccaag ccgcgccgaa acgcatcagc
    10741 gacaagtctt gttcgataag gggcaagagg ttggggcgca aaactttgac cgccactcgt
    10801 tcgcccgaat gcaggcgggc tttgtgtact tgggcgatgg acgcgctggc gacgggctcg
    10861 gtttcaaatt ccgcatacag cttttcgatg ggctgaccca gcgatttttc gatttgttca
    10921 cgcgaaagcc gcgcgtcaaa aggcggcact ttgtcttgca gctttgccag ttcgacggcg
    10981 taatcgtgcg gaatcaaatc ggggcgtgtg gacaaaacct gcccgaactt gatgaaaatc
    11041 ggccccaggc tttccaaggc aaggcgcaga cggacggcag gcggttcgtt tttcaatttg
    11101 gacgactgcg gcatcatttt cagcagagcg catatccaac cgctacccat cagcgaaacg
    11161 cacagacctg ccagccgata gcggtaaaaa gtcccgacaa tgaccgtcag gcgtttcaac
    11221 catttcatat ataaaatccc gaatacagca tttatcggac aatcaaaaaa tcttaccgag
    11281 cagatagccc aaaaaggcaa tcagcaaccc gaaaaccagc ggtttgatat tgcggggcgg
    11341 ctcgggttgc gaagatgcgg aaatatcatc ttcccgcccg ccgtgaagtt caagatattc
    11401 gggcatttcc ccatccgcgc ctcctccttc gccctccccc tcctccggcg gcacgaccgt
    11461 ttccaactcc atatacgggt aataatatcc aaacccaaat agctgaaata atcatgcaac
    11521 tcttcccgtt tcgcccaagt gcatttttcc tcaaccagca cttgcggttt ggaatcgagc
    11581 gattcgatca atccttccgc cttttcttcg ccgagattct ccttaaggca ctcccggata
    11641 agcccgcgat ccacttgggg cgggtaggaa acatatacat cgtacaaatc gtttcggtaa
    11701 tctttactca tctcaatcat aagggaacaa atacgcctga taccgttccg cccgttcctt
    11761 tcgggaaacc cgattcatct tcaagcaatc ggattatatg taaattcatc ccgataatat
    11821 attttccaaa agtaaaaatg ccgtccgaac gttcagacgg cataacacaa accagccggt
    11881 cacacggaaa acgacgatcc gcaaccgcag gttgtttccg cgttcggatt gcggatgacg
    11941 aactgcgaac cctgcagact ttccgtatag tcgatttccg caccgaccag atattgatag
    12001 ctcatcggat cgaccaaaaa gaccaaaccg tttttctcaa tttcaaaatc gtcgtcgttt
    12061 ttgatttcgt caaaagtaaa tccgtactgg aaacccgaac agccgccgcc gttgacaaaa
    12121 acccgcaatt tcaaatcggg attgttttct tcggcaatca aatcggcaac tttggcacag
    12181 cagctgtcag taaaaataat agggctttcg tccgacatag tgttattcct taatataata
    12241 ttttctgcat tctctctcaa ccccccttca aaggcaagca gggcttcgcc tttgaaaaag
    12301 gaattgtccc aatagataag tttgaccgcc tgcaatttca agccccaaac tcaaaccgtc
    12361 ctgccgaacc actcgacacg cccgataatc tccacatcat cggtcaagtc gttcaaattg
    12421 atttcaaaag caggataagc ctcgttggcg gaaatgacat tgataatccc gccggggacg
    12481 atttgcaggc gtttgaccaa cagattttca ttaatccgca acacatacag tccgtctctc
    12541 ggcgtatttt ccccatgatt gaccaaaatc gaatctccgt cattcaaaac cccctccatc
    12601 gaatccccct tgacggaaat tacagacagg tttttcgtat ctcgggtaac ataattctca
    12661 atccaatgcc gtctgaacgc cattgtaaat accggttcct catgaccgac aaactgcccg
    12721 tatcccgcag ccgcccgaat atcatatctc ggcacaaaga caaactcgtc cgtatcaact
    12781 tcatttccca aggtatcgta agcaagggat ttttttgggg cttcatccgg aaacggatta
    12841 ccctcccctg tcagcagcca atcgatacta caccccttca actgcttaat ttttttcaat
    12901 gtttcagact tcggcagacc gccttcattc catattctgc tgaagcccgc aatcgtcatt
    12961 tcaatatcgg atgcgatttt tgcctgcctc gcttcacttt tccataaaaa aaccagtctg
    13021 tctttaaagg tttccataaa tatccttccg ctccccttca ttcaatcatc taaagtaaac
    13081 atgaaatata ttttacatta gctatcccaa aaacggaata actatgataa actaaattta
    13141 aattatgatt tttcttatcc aatacataat ttttcagatt ttccataggt ttaatcccga
    13201 attgacagaa aaaggataga aaaatgaaga aaaccagcaa atatcttatc tatactgcgg
    13261 catttacctc attctgcttt gccttccaag aaaaccgttc tgaagccaaa cagcccgaca
    13321 tcactttatc cgcatccctg tgcgaacaat tcaacatgct gaacgccaaa gatatggata
    13381 cagaacaagt ctccctttcc aaagaatgcg acatcatcga gtcttcacac gactgggaaa
    13441 aagagtacgg caacttgaac gaacaggaaa tgctcgccgg cgtcgtctat gaataaacct
    13501 gcctgccatt tgaaacatta tgcttgaatg cattggagcc aaatgtatta aatcaaatat
    13561 aaaaccaata tattcataaa gttatatact tatagccatg ctgtagcttg aaacagcccg
    13621 ccgcccgccc tatttacagc ccatcgggac aaaatgttcc aacatatttc tcaaaataag
    13681 caaaatcaaa taggagtatc cacatggcaa aagtaatcgg tatcgactta ggtacaacca
    13741 actcttgttt ggccatttcc gaaaacggtc aaaccaaagt catcgaaaac gcagaaggcg
    13801 cacgcaccac gccgtccatt atcgcttatt tggacggcgg cgaaatcctc gtcggcgcgc
    13861 ctgccaaacg ccaagcggta accaacgcca aaaacactat ttacgccgcc aaacgtttga
    13921 tcggtcacaa atttgaagac aaagaagtcc aacgcgacat cgaatctatg cctttcgaaa
    13981 tcatcaaagc cgacaacggc gacgcatggg taaaagcaca aggcaaagag ctgtctcctc
    14041 ctcaaatttc cgcagaagtc ctgcgtaaaa tgaaagaagc cgccgaagct tacttgggcg
    14101 aaaaagtaac cgaagccgtg attaccgtcc ctgcctactt caacgacagc caacgtcaag
    14161 ccaccaaaga cgcaggccgt atcgccggtt tggacgtaaa acgcatcatc aacgagccga
    14221 ccgcagccgc tttggcattc ggtatggaca aaggcgacaa caaagaccgc aaaatagccg
    14281 tatatgactt gggcggcggt accttcgata tttccatcat cgaaatcgcc aacctcgacg
    14341 gcgacaaaca atttgaagta ttggctacca acggcgatac tttcttgggc ggtgaagact
    14401 tcgaccaacg cttgattgac tacatcattg acgagtttaa aaaagaacaa ggcattgatt
    14461 tgaaacaaga cgtaatggct ctgcaacgtc tgaaagaagc tgccgaaaaa gccaaaatcg
    14521 aattgtccag cggccagcaa accgaaatca acctgccgta cattaccatg gacgcaaccg
    14581 gcccgaaaca cttggcaatg aaaattaccc gcgccaaatt cgagagcctg gttgaagacc
    14641 tgattgcccg ctctatcgag ccttgccgca ccgcattgaa agatgccggc ttgagcaccg
    14701 gcgacatcga cgacgtgatt ttggtcggcg gtcagtcccg tatgccgaaa gtacaagaag
    14761 ccgttaaaga cttcttcggc aaagaaccgc gcaaagacgt gaaccctgac gaagccgttg
    14821 ccgtaggtgc agcgattcaa ggcgaagtat tgagcggcgg ccgcagcgac gtattgctgc
    14881 tggacgtaac ccctctgtcc ttgggtatcg aaaccatggg tggtgtgatg accaagctga
    14941 ttcaaaagaa cactactatt ccgactaaag cgtctcaagt gttctctact gccgaagaca
    15001 accaaagcgc agtaaccatc cacgtactgc aaggcgaacg cgaacgcgct tctgccaaca
    15061 aatctttagg tcaattcaac ttgggcgaca tcgcacccgc accacgcggt atgccgcaaa
    15121 ttgaagttac tttcgacatc gacgccaatg gtatcttgca cgtttccgcc aaagacaaag
    15181 gcactggcaa agcggccaac attaccatcc aaggttcttc aggtttgagc gaagaagaaa
    15241 tcgaacgcat ggtgaaagat gccgaagcca atgccgagga agataaaaag ctgactgaat
    15301 tggtcgcttc ccgcaaccaa gccgaagccc tgattcactc cgtgaaaaaa tctttggcgg
    15361 actacggcga caaactcgac gctgccgaga aagaaaaaat cgaagccgcg ctgaaagaag
    15421 ccgaagaagc cgtgaaaggc gacgacaaaa ccgccatcga tgccaaagcc gaagcactgg
    15481 gcacagccag ccaaaaactg ggcgaaatgg tttacgcgca agcgcaagcc gaagcccaag
    15541 ccggcgaggg cgcacaagcc aatgcttctg caaagaaaga cgatgatgtc gtagatgccg
    15601 actttgaaga agtaaaagac gacaaaaaat aattgatgcc gtctgaaaaa aacgcgaacc
    15661 attcggttcg cgtttttttc aattgagata aaagacaata gcatgacaga gattccaaat
    15721 tcatctaccg tgatattccc aagcccaagt tccaactgtt gcatcggtta tctggaaatt
    15781 tttcatatca ttttcttatt tacttaaatt ttttaataag ataataaata ataattatta
    15841 tcatgagcta agaaatgaaa ctaaatactc tcacatgggc tttgatgacc gttttttccg
    15901 ttgcgccatc ttgggcagaa caaccggcaa atactgaaga aatacaaccc gtcaaaacct
    15961 tctccccgcc caaaccgatt gcaccgaccg ccgcacaagg ctatttcccc gaaaaccaat
    16021 tcgaccgctc cgaccgcagc gattattact ttgttaccga aaacatagac caagccttcc
    16081 gtccgctgaa ggcaaacagc agtttttacg gcaaaagctt ttacaactcc gttaccgcgc
    16141 aagcactcgg ggcgaaggta tacggcgtag ccaacctcaa tcgcaccaag gcaaacggct
    16201 acaaagatgg cggtgggccc gacaccgatt ggaaatacag ccgcttcaat caggctttgg
    16261 tactcggttt cgtgccgtct gaaaatcaag aataccgcct cacttatctg cacgacgaca
    16321 tcaacaacga ccgccagccg caggtcgtca acgacgcatt ggacaccgaa cgccacatct
    16381 ccaaactcaa cgtgcgttgg ggcaatgccg atttgagcaa tacggtcagc gcggaagcag
    16441 gcgtcatcaa actcaaacgc catgccgaca attactcctt gcgaccaaac aacacgccgc
    16501 agcaagtgtt cgtagaactt gaccgcaaag tgtatgattt ctcgctcaaa cacgatgcgg
    16561 atttcggcaa attccacaac acggcagccg tcagctaccg caacgacagc caaaacggcg
    16621 aacgcaacac ccacactgcc atgtgcgatt tcctcaacgg ctaccgtttc gccgatgtgc
    16681 acatcgaccg ctggtgcatt gccgatacct tatcttacaa gtttgacgac cgacacaaac
    16741 tgggcttggg cttgggctta agctacgaac tcaacgaagc ggacattcgc aaaaatacgg
    16801 cacagcctga aaacccaata aaacccggtt tccccttcgc ctcatcacaa caaatctgga
    16861 aaacccatta cggctacgac ttcaacggca aagtgcgccg tcatgctttg tcgggcgaac
    16921 tcaaatacga tttcacgccg tctgaaacgc aaaaatacag cgtttccctc gcgcatttgg
    16981 aacgcatcgg cgacaacacc gaacgcttca actcgcttgc tgccatcgtg caaaaccgta
    17041 tgagcggcat gctgatgaac caaaatccag ccgccgccat tgcaggcaat ccccctgctg
    17101 aaaactgaaa aacacaaccg catcaggctc accgccgaca gccgcaacga ctactacaac
    17161 ggctacatga actcgctcgc aggcgcgggc tggaacgtgg gcggcacgct tgtggcggac
    17221 aaagtcaaag acctgattat ttttgaccgc gcacacggac aaagcggcac agcttccaaa
    17281 gacggcggca tcatcacacg caacgtggac gcacgattgt ttaccgcgca ggcctacgcg
    17341 cgttacaact tcaatccgca ttgggcggca ggcatcaaag ccgcctacaa ctacggacac
    17401 aacgaaaccg acggcaggcc gccctatcaa atccgtccgt ttgaagccgc cgtccaagcc
    17461 gactacaaaa actactttgc ccacggcagc tacaacatcg gcgcggcaac acgctttgtc
    17521 gccaaacaaa cgcgcggtga ttttgatatg gcaagcggtc tgggcataga caaacgcgaa
    17581 gccgccaaag gctttaccgt tgccgacgtg tacgcaggcg taaacatcaa agacaaatac
    17641 ggcttgcgct tgggcgtgaa caacgtattc aacaaaaaat acgtcgaata catcagcggc
    17701 gaccacgtcc tcgccctatc ccccagcgta gtgtatgcac cgggcaggac atattggttg
    17761 agtttgcatg cggcatttta aaaccatgcc gtctgaaaaa tacctgcatt ggcttattaa
    17821 gaattaggag ataaaaaatg gatatgaaaa gacgcgattt cttaaaaatg accgccgcgc
    17881 tggcagccgc aggcgtttcg ccttctctgc ttactgccgg taaagagcaa tttaccgtgt
    17941 acggcgcacc ggcaatgccc agcgttacca ttgccgtaac ggcgttgcaa ggtaaactgg
    18001 cgaaacaggc ggacgtgtcg ctgaaaatct ggcgttcgcc cgaccaactg cgcgcgggcg
    18061 tggcaagcgg gcaatttaaa gtgatgatga gtccgagcaa tgtcggcgta aacctgcgca
    18121 accaagggca gaaagtcggc atggtgaata ttttgaccaa cggcatcacg cagttggtct
    18181 gcaaaggcag cgcgattgcc tcgccgcagg atttggtcgg caaaaaaatc ctcgtgccgt
    18241 ttaaaaacga tatgcccgac atcgtgctgc aagccttgtt gaaaaaactg aaaatcgacg
    18301 cacacaaagt cagcattacc tacgctgcca caccgcccga agcagtcgga ctatttccaa
    18361 gtaaaggtta ccatgccgtc atcctgcccg aaccgatggc aaccgccagc ctactgaaag
    18421 gcaaaaccat aggtataaac gtcgtgcacg gttttgattt ggtaaaagca tgggggcagg
    18481 cgtttggcac caaaccgctg attccgatgg caggtatcat cgccaacgaa gaatatttcc
    18541 acgcacacaa ggcgcagttc gacatcttcc atcaagattt gaaaaacgcg ctcaactgga
    18601 tattggccaa ccgccaaaac gcggcgaaaa tcggcaaaaa ctacctaccc gcccccgaac
    18661 ccgccctagt catgggcttg gacggcgcac ggctgacggt aagcaaaggc agcgaagtga
    18721 aaaacgagat tttgaagttt tacgaaatcc tgatgcagtt caacccggaa cttttgggcg
    18781 gcaagctgcc ggataacggg ttcttcttgg cttaaaggga aagaggccgt ctgaaaaggg
    18841 tagggtggtt gtgaatacct gatttatcat ttttcagacg gcctattaag gcattgtcag
    18901 cggcgtgggt cggattcttg aatccgacat ttcggctaaa cggggctgtc ggatacaagt
    18961 atccgaccta ctcttttcag acggcctatc agataatagt tcgaacagta cacaaagagc
    19021 cgtcggattg cctgaaatgt tgggtcatga cccaacctac gcttactggg gaaacgggat
    19081 cgaagtgtga ggtatgtggc ggggttgttt aataatgaaa ttttataact atttgatagg
    19141 gtggtaatgc catgaatgaa gataaatata gggaaaatat gacatgggat gaaattgaaa
    19201 atgtagtagg aactttagtt tctgaagaaa gaatgcttaa atccaatagc tttttaagta
    19261 ttaaaaatag taagaatagg ttggcttaat cacaaccata acccaacgaa aatactaaaa
    19321 ttccaagcta aggctatcct ttccaaacca tccccatcaa ccaaaccatg attaaaaccg
    19381 acaaaatccg caaaccgcag cctgcgctgt tttacatcat cgactacctt tggagcggct
    19441 ttgccggtct gagcgtggcg atggcgatgg tggcgttgtg ggcgtggggc agcgccgtgt
    19501 tcggcgagtt tatgctgcct gcgccggtag aggtgtttca aaaatctttg gatttattga
    19561 aacattttca ggaaaatgaa atcgggattt cgctgtggcg gtcggtagtg ggtatttcgg
    19621 ttgctttgat agcaggattg gcggcggggc tggtggcagg cagttttaag acggcgatgg
    19681 cgttgcttaa gcctgtgatt acgattttgt tagcaatgcc gccgattatt tgggtggtga
    19741 tggcgttgtt ttggttcggg ttcggcaatc cgagcgtgtt gtttaccatc attgtgttgg
    19801 ttgcgccgct gacgtttgcg agtgcggcgg tcggaatggc gagcgtgaac aagcagcatg
    19861 aggagttgtt tgacgcttat aaattaggcc gtctgaaaaa aatccgctat ctgtatatcc
    19921 cgcatctgac gggctatgtg atttccagcg tcggcgtggc ggtggcgacg ggggtgaagg
    19981 cggtgatcat ggcggaactc ttaggcgcga gcaaaggcgt gggcgcgcgg attgcggacg
    20041 cgagggcaat gctgtagact tcgacggtaa tggcttatgt ggtgttggtc atggtatttg
    20101 tgtcgctgtt tgaatacctg attccaagcc tttgtaaatt ttgtttatgc cgtggaggag
    20161 atgatgctct gtcttgaaaa cgtgcgtttt gaaatcctcc gcgaccccat cgtgcgcgat
    20221 ttcagtttga acctgcaaca gggcgaagtg aaagccttgt tcgggccgag cggctgtggc
    20281 aagacgacgg ttttacggct gattgcgggc ttggaaacgc cgaaatcggg cacgatacgt
    20341 aatactttcc acaaaacggg ttttctgttt caggaaaacc gcctgccgga aaacctgacc
    20401 gcgatgcaga atatcgcgat ttttatggac aacccagacg aaggcgaaat cgtcgcgctg
    20461 gcggcgaaag tcgggctgac tgcgggcgat ttgaacaaat atccgaccga attgtccggc
    20521 ggcatggcga aacgggtggc gtttttgcgc ctgctgctgt gcggctgcga cctagccttg
    20581 ctggacgaac cgtttgtcgg tttggaccgc gatttgcgcg atattttggt tgctatgctg
    20641 gtggaaaaaa tcgagcggca gggcatggcg tgtatactgg taacgcacga ccgcttcgaa
    20701 gccgcgcgcc tgagccatga aattatgctg ctttccgcta agggcatgaa cgtgcaaaac
    20761 gtgataaccc tgcccacgcc gctgtccgaa cgcgattcgg cttttgaaga agtcgtggtg
    20821 gcaagggagt ttcaggggat tcattattat gagtgatgag gggatttgag tgtctgacaa
    20881 accttacggt tcgttgccct ctccctaacc ctctcccacg gggagagggt tagcaagcct
    20941 caggcttgcc tctttagcga aagatacgaa tctgttatcc gaacgcgatt cggtttttga
    21001 aaaagccgtg gtgccaaggg ggcaggggat tcattatgag gtgctttatg ttttcgactg
    21061 tgattactgc tgctgtttta tatattgcta cagcagtaga tttgttggta atactattaa
    21121 tattttttgc tagagcaaat actagaaaag aatatcgaga tatttatatc ggacaatatt
    21181 taggttctgt aattttaata ttagttagtt tatttctagc ttttgttttg aattatgttc
    21241 cggaaaaatg ggtgttgggt ttattaggtt taataccgat ttacttaggt attaaagttg
    21301 ctatttacga cgattgtgag ggcgaaaaaa gagctaaaaa agaattggat gaaaaagggt
    21361 tgtcaaaatt agtcggtatt gttgctttgg ttacagttgc tagttgtggt gcagataata
    21421 ttggactttt tgttccttac tttgtgactt tagatcttgt cgacttatta gttactcttc
    21481 ttgtattttt aatattgatt tttgttttag tatatacagc acaaagattg gctaatattt
    21541 caggtgttgg tgaaattgta gagaagttta gtcgttggat aatggctgtt atttatattg
    21601 gtttagggtt atttattatt attgaaaata atacaattcg aacgataata tcaataatat
    21661 gaatgacaag ggcatttcag tatctgacaa accttgcggt tcgtttccct ctccctaacc
    21721 ctctcccaca ggagaggggt tcaggttgct gaaaatcaga gggcgaagga ttggggatca
    21781 aatggaaggt aaaacctgaa aatgttcggg tttgacagcc cgattccctc tccccgtggg
    21841 agagggctag ggagagggca agcaagccgc aggcttgcct ctttggcgaa aaatacaacc
    21901 ctgtccgccc aataaatcca tacccgaaag catcttcata cagaatcaag ccaagcgatg
    21961 aataaatttt ttacccaccc tatgcgcccg tttttcgtcg gtgcggcagt acttgccata
    22021 ctcggtgcgt tggtgttttt tcatcaaccc cggcgctatc atcctgcacc gccaaatttt
    22081 cttggaactt atgctgccgg ctgcatacgg cggttttttg actaccgctt tgttggaccg
    22141 gacgggtttt tcaggcaacc tgaaacctgc cgctactttg atggcggtgt tgttgcttgt
    22201 tgcggctgtt ttattgccgt ttttaccgca acttgccgca tttttcgtcg ccgcctattg
    22261 gctggtgttg ctgctgttct gcgcctggct gatttggctc gaccgcaaca ccgacaactt
    22321 cgccctctta atgctgctcg ccgcattcac cgtttttcag acggcctatg ccgtcagcgg
    22381 cgatttgaac ttaactgcgc gcgcaagtgc atttgaatat ggcggcggtc atgttcgtat
    22441 ccgtccgcgt cagcgtcctt ttgggcacgg aaaccctgaa agaatgccgt ctgaaagacc
    22501 ccgtattcat ccccaacgtt atctataaaa acatcgccat caccctgctg ctgcacgccg
    22561 ccgccgaact ttggctgccc gcgcaaaccg ccggttttac tgcgcttgcc gtcggcttca
    22621 tcctgctcgc caagctgcgc gaactgcacc atcacgaact cttacgcaaa cactacgtcc
    22681 gcacttatta cctgctccag ctctttgccg ccgcaggtta tctgtggaca ggcgcggcga
    22741 aactgcaaaa cctgcccgcc tccgcgcccc tgcacctgat taccctcggt ggcatgacgg
    22801 gtggcgtgat gatggtgtgg ctgactgccg gactgtggca cagcggcttt accaaactcg
    22861 actacccgaa actctgccgc atcgccgtct ccatcctttt cgcctccgcc gtttcgcgcg
    22921 ctgttttaat gaacgtgaat ccgatattct tcatcaccgt tcccgagatt ctgaccgccg
    22981 ccgtgttcat gctttacctg ctgacgttcg taccgatttt tcgagcgaac gcgtttacag
    23041 acgatccgga ataaaaatgc cgtctgaaac ggttttcaga cggcatcgga ttgccaatga
    23101 tcaagcctcc tgcggatcga catccaccga ccatcggatt ttgccgtcgc ggttctgctg
    23161 caacacctgc acccacaaac tcacggcgcg gtgcaaatct tgtcgggatg ttgattcgag
    23221 gaaaacttgc gcacgttcgc gttcggcaag gcgcaccatc agcatcgggg cggcaccgaa
    23281 ccgggagacg ctttcgggca agagcggggc gagggtttct ttggcggcgt tgagaaattc
    23341 catcgcatcg gcaacacgcg gcgcatcggc acggacggcc gtctgaaaac cgaaaggcgg
    23401 catggcgaac atttgccgtt cgttcaattc gttttcggca aacacggcgt agtcctgcgc
    23461 tttgacggcg gcgaagacag ggtgttcggg cagttgggtc tgtatcagca ccttgccggg
    23521 tttgtcggcg cgccccgccc tgccggacac ttgcatcagc tcggcaaaca gcctttccgg
    23581 cgcacggaaa tccgcgctgt acaggctgcc gtcggcattc aacacgataa cgaggttgag
    23641 ccgcgcgaaa tcatggcctt tggcaagcat ctgcgtgccg accagaatgt cgatttcgtc
    23701 gttggcgatg cggcggtaca aatctgccca gtcgtttttg tgcgccgtac tgtccctatc
    23761 gacgcggacg acggctgcct tgggcagcaa ggcgcgcagg gtttcttcga cgcgctgcgt
    23821 accgtgtccg acggcggtca ggtcttggtt gccgcagtcg ggacatttga acgggatggg
    23881 ttcgcggtgg tcgcagtggt ggcagcgcag ttggcgggcg cgttggtgca gcaccatttt
    23941 ggcggagcag ttcgggcagc cgaaggtatg gccgcagtcg ccgcaaaaca gcgcaggcgc
    24001 gaagccgcgc cggttaaggt acaccagcga cataccgccc gcttcaaagt tctgtttcaa
    24061 aagctgcaag gcttgcggtg agaagccgtt atcgagtttc agacgaccta tgttgaggat
    24121 gtccacttgt ggcagttgcg cggaagcatg ggcgcgttcg gtcagttgca gcaggcggta
    24181 cgcgccgctt tgcgccttgt gccaactctc caagctgggc gtggcactgc ccaacacgac
    24241 gggacagccg ccctgcttcg cccgccacac cgccaaatcg cgggcgtggt agcgcaattc
    24301 gttgtcttgt ttgaacgagc cgtcgtgttc ctcatcgacc acaatcagcc cgacatcagg
    24361 caaaggcgta aacaccgcca gccgcgtgcc gatcaccagc ttcgcctgcc ccaacatcgc
    24421 gcgcagataa tcctgcgtgc gcctgcctgc cgccatccgg ctgtgcaaca cggcggtcgg
    24481 cacgtcggca aaacggtttt ccacccgctt taaaagctgc ggcgtgaggt tgatttcggg
    24541 caacagaaac aacacctgtc gcccctgcgc caacactttc gccatcgcat cgaaatacac
    24601 ctcggtcttg ccgctgccgg taatgccgta cagcagaaac gaccggaagc gtcccaatgc
    24661 cgtctgaatt tcatcggaag ccttctgctg gccggtattt aatacaaatt cagagtgcga
    24721 agcctgcccg cggtacggcc ttaaaacagg tttcgccgct tccgtcgttt caatccagcc
    24781 ctgctccgcc caatcctcaa tcaatttcgc cgcctgcgcg tttgcctgtt tcaacgccgc
    24841 catcgtcatt tcgcccgaca gcagcgcgtc ccacaaagcc gcttttttgt tgaaccgagc
    24901 cggcggcggc gtttgcgccc tgcccgcttc gttcaaagca taaaacaacg gcggctgcgg
    24961 catttccacc gcgcgcgttt ccttcaaacc ctgcggcaat gcggcaaata cagcctgccc
    25021 agtcggatag tggtaataac gcgacgtaaa cgccaacaaa tcacgccagc tttgagacag
    25081 cggcttttct tccacaaaga ccgtctgaac gctcaaaatc cgcgccgcat ccatatcggg
    25141 cgcaatatcc gtttcccaca ccatcccgac aacagtttta ttgcgaaaag gcacaagcac
    25201 ccgcgttccc ggaggaagcg gctcggaatg cgaataagtc aaaaggccgt ctgaaagcgg
    25261 cacgtttaca gcgatgcgat ggtagatcat acaggcaggc aaatcatttt tttgcgtatt
    25321 ttaacagccg gaagctattt tcagacgata aattgagcct cggcgcaatg caggcgcaac
    25381 agaggtttga tacaatgcca cttttccaac acgctcaatc atctttgata tgaaaaccaa
    25441 gttaatcaaa atcttgaccc cctttaccgt cctgccgctg ctggcttgcg ggcaaacgcc
    25501 cgtttccaat gccaacgccg aatccgccgt caaagccgaa tccgccggca aatccgttgc
    25561 cgcttctttg aaagcgcgtt tggaaaaaac ctattccgcc caagatttga aagtgttgag
    25621 cgtcagcgaa acaccggtca aaggcattta cgaagtcgtc gtcagcggca ggcagattat
    25681 ctacaccgat gccgaaggcg gctatatgtt cgtcggcgaa ctcatcaaca tcgacacgcg
    25741 caaaaacctg accgaagaac gcgccgccga tttgaacaaa atcgacttcg cctccctgcc
    25801 tttggacaaa gccatcaaag aagtacgcgg caacggcaag ctgaaagtcg ccgtcttctc
    25861 cgaccccgat tgtccgttct gcaaacgctt ggaacatgag tttgaaaaaa tgaccgacgt
    25921 gacggtttac agctttatga tgcccattgc cggcctgcac ccagatgccg cgcgcaaggc
    25981 gcaaatctta tggtgtcagc ccgaccgtgc caaagcgtgg acggattgga tgcgtaaagg
    26041 caaattcccg gtcggcggca gcatctgcga caatcccgtc gcggaaacca cttccttggg
    26101 cgaacagttc ggcttcaacg gcacgccgac cctcgtcttc cccaacgggc gcacccaaag
    26161 cggttacagc ccgatgcccc aactggagga aatcatccgc aaaaaccagc agtaaacccg
    26221 caatgcaaaa ggcttggaac catgttccaa gcctttttca aatgccgtct gaagcctgtc
    26281 gcggcagctt cagacggcat ctttgttcaa acctcaatcc tgcgccaatg cgtctatcgg
    26341 attgagtttg gctgccttat tggcgggcat aaagccgaac gcgatgccga ttccggtcga
    26401 acaggcgacc gccccgataa cggatgccgc cgaaatgtcc atcgggaaat cggttacaaa
    26461 atgattgaac acgaggctga cggcggcgga caaacctacg ccgaccaagc ctccgatgat
    26521 gcagattaac accgcctcaa tcaaaaactg ctgcaaaata ttgccgcgcc gcgcgccgat
    26581 tgccatccgt atgccgattt ctttggtgcg ctcggtaacg gacaccagca tgatgttcat
    26641 cacgccgatg ccgccgacta ccaatgaaat cagggcgatg gaggaaatca gcagcttcat
    26701 cgtaccggtg gtgctttcga ccatctgcct gatgctgtcg ctgttgttca taaagaagtc
    26761 ttccgtgccg tgccgtgctt tgagcagctc ggccagccct ttttcggcaa cccgggtatt
    26821 ggcattgtct ttgattttga cggtgatgga gttggtgtgg ctctcgcctg tgatttggtg
    26881 catcaccgtc gtatagggcg accaaagcat cagcacgtcg gaattgccga aagcgttttc
    26941 gtcttttttc atcacgccga tgacggtcaa ggggcgtttc ctgaacaaaa tggttttacc
    27001 caacggatcc gagtccgcaa agagtttgtc tttgacattt tggtcgatga cgacgacttg
    27061 cgcgtcttct ttcacatcgt tctcatcaaa cagccgcccc gtttccagct tcagcccgcg
    27121 cacgtcgaaa tattgttcgc ccacgccgta caaagaagcg gtcaggtcgg tattgcggta
    27181 ggtcagcgtg ccgccgctcg aagtcatggg cgtggcggag gcaacgtagc tttgtttggc
    27241 gatgattttt gcgtcgtcta tggtcagggt tttgattttg ccgctgcgcc tgtcgccgaa
    27301 gccgcgcccg gggaagatgc tgatggtgtt cgtccccatc gaactgatgt cttcgaggat
    27361 ttttttctgc gaaccgttgc ccaatgcgac gacggagaca accgaagcga taccgatgat
    27421 gatgccgagc atggtcagaa gcgaacgcat tttgtgcgcc aatactgctt gcaccgacat
    27481 tctgaaggct tcgacaaact ggtcgtaata aaacgaccac gaggcttttt cctgaatcct
    27541 cccgacattg cttgcgggga tttcgggatt tttcgaggtg tcggaaatga tttcgccgtc
    27601 ccggatttcg atgacgcggt tggcgttggc ggcaatgccg gggtcgtgcg tgaccataat
    27661 gacggtatgc ccggcttcgt gcagcctgcg gatgatttcc atcacatttt tgccgctggc
    27721 ggtatcgagt gcgccggtcg gctcgtcggc gaagatgatt tctccgccgt tcatcagggc
    27781 gcgggcgatg gagacgcgct gctgctgtcc gcccgagagt tcgccgggct tgttgccctc
    27841 tttacttgcc aaacccaaat cctgcaagag tttgtccgcc cgcgcggaac gctctttgcc
    27901 gcccataccc atatacacgg ctggcagcgc gacgttgtcc cttgcggtca gcgagcttaa
    27961 gaggttgtag cgttggaaga tgaaaccgaa gcgttcgcgg cgcaatgccg ccagttcgtc
    28021 aggctgcatt ttggcagttt cgatgccgtc gattcggtac gaaccggaac cggcggtatc
    28081 caaacaaccg agtatgttca tcagcgtgga cttgcccgaa ccggactgcc cgatgatggc
    28141 gacaaaatca cccttctcta tcgacaggct gatgtctttc aaaatatgga cgcggttctc
    28201 gccgctgccg aagcagcggt tgatgttttt acattcgatc aagctcataa tattccgtaa
    28261 atcaataaca aatgccgtct gaaaccgttt ccgtgttcag acggcatatt cgtttatcgg
    28321 cgcggcgggc cgcctagggc gcgttcgccg ctttcctgct gctcggcggc ggttatttcg
    28381 gagatgacca ctttgtcccc ctctttcaac ccgcttttca cttcggtatt catactgtct
    28441 ctcataccgg tccggatttc gcgttccgcc gccttgccgt ccgcacccaa cacgcgtacg
    28501 aacgccttgc cgccgcgatt tttcacggtc agcgacggaa taagcaacac atttttcaca
    28561 ccgtcgattt caaccgtatt ctgcgtcgtc atccccgtgg cgagtttgcc gtccggattc
    28621 ggcacaaacg aacgggcata ataatagacc gcattggaag ccgtatccgt actgctgttg
    28681 tagccgcccg acgacatcgt ggtcagcccg gggtcgacgc tgtcgagctt cgccttaatc
    28741 ggcgtatccg gttcggacaa aatcgtaaac gaaatatcct gccccgcctt caccttggta
    28801 atatcgccct cggcaatctg cattttgttc aacatcatat ccagattcgc caattggaca
    28861 atcgtcggcg tagactgcgc cgcgttcaca gtctgcccct cttccacggg aatcgccacc
    28921 accgtgccgt ccatcgtcgc ggtaatgcgc gtgtagccca aatccgactc ggcggtattg
    28981 atggaaattt tgctctgtct gattaaagcc ttcaactcgg caacattggc tttggcggcg
    29041 gcaagcgcat cctgcgcgct ttccaaatct tctttagagg tcgcatcatc cttccacaac
    29101 gccgcctgac gcttatattt cttctccgcg ctgcccaatg caatctgtgc cgacaccagc
    29161 ttcgcctgat acgtttccaa tttggatttt tccatatcga gcgtgttggt ctgcgtggtc
    29221 gaattgattt ccgcaatcaa atcgcccttt ttgacctgtt gcccgagttt gacataaagc
    29281 tttttaatct gccccgaagc ctgcgcgccg accgatacca ggttggacgg cgaaatctcg
    29341 cccgtcgcgg aaaccgtccg gctgatgccg ccgcgcctga ccgtttccgt aatataagaa
    29401 gcctgcggct cgggcttcag ataatgccat ccgccccaaa ccgctgccgc cgcgactgcc
    29461 gcaacagccg accatttcat cattttcgcc atattttcaa ttctctgtat gcgtcaatcc
    29521 cataaaccgg aagcggattc taccgcaagt ccctgcccca tccaaggcgg caaaccgccg
    29581 acaataaaaa taaccatttg ttttaaatgg cttatacaat ctgctttccc gaaaatttca
    29641 atccgtttac atattccgcc atttccgata cggcgttata atgccgccca caaacaacca
    29701 aatggaaaca cattgctatg aaaaacgttc aaaaaggctt tacgctgctc gagctgatga
    29761 ttgccgtcgc catcctcggc atcctgacgc tcatcgccta cccttcctat aaaacctaca
    29821 tccggcgcgc ccgcctgtcg gaggtcaaat ccaccctgct ggtgaacgcg caaaacttgg
    29881 agcgttacta ccgccaaaaa gggacgtttg aaaaatacga ctcaaccaaa ctgaaacaaa
    29941 acaaatattt caagattacc ttaatcaaag ccagccccga ccacttcacc cttcaggccg
    30001 ctcccgatac ggcgaccaac gaaggcgaaa cctgcaccgt cacgctcaac gacggcggta
    30061 ccattgccgc ttccggtaca aaccaatcct gccccggctt cgactgattg gctgttgcag
    30121 tcggcattgt gcctaaacca tctataaaaa tgccgtctga acgctgttca gacggcattt
    30181 ttgacatccc gatacgcctt tcaaacggct tccgccattt ccaacgcctg cttgatgtct
    30241 acggcgacga cgcgcgacac gcctttttcc tgcatggtta cgccgaccag ctgctccgcc
    30301 atttccatgg tcaggcggtt gtgggagatg tagaggaact gggtttgcac cgacatttct
    30361 ttgaccagct tgcagaaacg cgaggtgttg gcatcgtcca gcggggcatc gacttcgtcc
    30421 agaaggcaga agggggcggg gttgaggctg aacagggcga acacgaggct catggcggtg
    30481 agggcttttt cgccgccgga gaggaggtgg atggtgctgt ttttcttgcc gggcggacgc
    30541 gccatgatgg acacgccggc ggtcaggagg tcgtcgccta tcattttgag ggtggcttcg
    30601 ccgccgccga acagggtcgg gaagaaggtt tggactttgc cgttgacggc gtcgaaagtt
    30661 tctttgaaac gcgctttggt tttgtcgtcg atttgggcga tggcttcttc caaaagcgcg
    30721 atggcggcct gcacgtcttc gctctggctg cggtagtagc cgtcgcgttc gcgcgcttct
    30781 tcgagttctt gcagggcggc gaggttgacg gcgccgaggg cttcgatttg ccgggtaagg
    30841 ctgccgatgc tgctgttcaa tactttcgcc gattctttcg ccaacgcttc gagcacgtcc
    30901 agatcggcag cgcgttcggt caggttttga tggtagcgtt tggcgttgat cagggcctcc
    30961 tgctgctgca acagggcggt ttgggtggcg gcctgaagct gcggcagctt ggtttgcagg
    31021 gtttgtacgc gggcgtattg ctccctgccc tgttcctgaa tctgcgcgag tttttcctgc
    31081 acggcgatgt attcttcgtc cagcgtgtgt acggtttcgg tcaactcgtc gagcttgatg
    31141 tgctgctcgt cgttttggaa ctcggtttca taggcgaggg caagctcttg ccggcgttcc
    31201 tgccagtcca gggtttgctg ttcgagccgg gcgatttgct gccggtagtt ttgtttttgc
    31261 tggttgagtt tgtggacggc gacttcggca agcccgtatt ggcggttggc ttccaacagg
    31321 gcaagctgcg cctgtttcag acggccttgc tgctcttggc ggctgtgcgc ggtggtttgc
    31381 tgctggtgtt cgagttcggc ggcggcttcc tgcaaagtag cgatgtcgtc tgaaagcccg
    31441 tcggacgtgt gttgcaacac ggtctgctct tccgccaact gtgccagttc gcgctcgatg
    31501 tgttcgcggc ggatttgccc ttggttggtg cgcgccagaa gttcggcggt gcgttgctgc
    31561 gcttggctgt attggcgcgt gtgttgctgt tgctgctgca tcagattttt gtgctgcact
    31621 tcggacgagc gcacggcagc ctccgcctgt ttgaacgcgg cttcggcggc ggaaagtccg
    31681 ggggcgaggt tttccagttc ggacgcgatg ccgtcgaggc gcgctttttg ggcaatcagg
    31741 ctttcctgcg cgggcttggc atagagcagg acgctgactt tatcgacctg atggccttcg
    31801 ggtgtgagcc agatttgatg cgtgcccaaa tcgctttgat gcgcgagggc gtagctcaaa
    31861 tcgggcgcgc acaatacgcc gtcgagccag tggtgcaatg ccgtctgaaa cggcggctgc
    31921 gcttggattt ggttcagcaa tgcctgtacg ggcagggatt ttttgatgcc gccggagagg
    31981 ccgtctgaaa gccatgccgc ctgcccctgc ggcaaaggtg cgggcggtac gaaactattc
    32041 ggcacgctac gggcgtgcag gcgttcggca aggatgacgg acagggcgtg ctgccactcg
    32101 gcgggcgcgg tgatgtgttg ccacagttgc ggcgcggcgg cgtggtcggt tgcctgccaa
    32161 aagtcggcgg cttcctgctg ttgcgacagg atttgcgaca acgcctgctg ctgcgcctgc
    32221 aaggtaatgt gttgctgctt caggctttgg aagcggtttg aggccgtctg aaacgcctcg
    32281 cgggcggcgt gcaaggcttc ttcggcggca atgatttgtt cttcgtaatg ctcttgccgg
    32341 ctttgcaaca aggcggcggc ttcctgagcg gcggcggttt cggcctcgtc gggcaggttc
    32401 agggcttggt tttcctgctt cagacggcct ttgcgctctt cgtgcttggc aacggtttgt
    32461 tcggcatggg caagctgctg ctgcttcaac gccagttcgc ggcggatgcg gtttgcctcg
    32521 tcctgctggg tttggaaggc agcgttgagc gtggcttgga cttcttccaa ttcgggcaga
    32581 cgctcttcgt gttcggcaac ctgcatcgcc cattccgcca attcggtttg cttttcttcg
    32641 acctgcaact cgttttcctc aagctgcacg cggatttgct gctgctcctg ccggatgcgt
    32701 tgcaactgcg cctgcgccgc ctgcttgtcg cgttcgatgc gttggtgcag gttttgccgg
    32761 tggcggattt gttcttccaa acgggcaatc tgctcgcgca acacgccgcg tttgttgctc
    32821 aattcgtgta ctgcctgctg ctgcgactgt tcggcagtct gcaaggcgtg tacttcgtta
    32881 tttaacgcct gaacctgtgc ggcggtttca tcctgctgcg cctgcaaaga ttgatgctgc
    32941 gcggtcgcct tgtcggcggc ggcaagcgat tgctgccatt gggcgtaatc gagcaaatcc
    33001 tgttgttgat tcaactgcgc ggtcagggat ttgtagcgtt cggcggtttc cgcctgtttt
    33061 tccagctttt ccacctgacg cgccaactcg ttctgcaaat cgcccaaacg ctgcaaatgc
    33121 tcgcgcgtgt ctttcagacg gccttccgtc tccttgcggc gttccttata tttggacacg
    33181 cccgccgcct cctcgatata ggcgcgcaac tcctccggcc gcgcttcgat gatgcgcgaa
    33241 atcatcccct gctcaatgac ggcataaccg cgcgcgccca cgcccgtacc caagaacaaa
    33301 tcggtaatat cgcggcggcg cacggtctga ttgttgatga aataagtcga ttccccctga
    33361 cgcgtcagct gccgcttgat gctcacctcg gcatactgcc cccacgcgcc ctgcaaactg
    33421 tggtcgctgt tgtcaaatac cagctccacc gaagccctcg gcgcaggacg gcgcgtcgcc
    33481 gcaccgttaa aaatcacgtc ctgcatactc tcgccgcgaa gctgcttcgc cgaagcctcg
    33541 cccaacaccc agcgcaccgc gtcaatcaca ttcgacttgc cgcagccgtt cggcccgata
    33601 accgcgacaa gctgccccgg aacatgaatc gtggtcgggt cggtaaaaga tttgaagccg
    33661 gagagtttga tgtgggtcag gcgcataatg gtcggaaaaa ataaaaaaga agcgtatttt
    33721 aacggaaatc ccgccgcacc accccatatc ttgccggcaa aaccttaccg catcccgccc
    33781 cggtgcggca ggattttgca catcccggcc atccgtatta aaatctttgc gtttcccaca
    33841 ctttcctgac aaatatacgg aggaggattc catgaaaaca aaaacagtca agcaagcaag
    33901 caagcaagca agcaagcaaa atagtctaac ccgcctttct ttaatcgctc tacttaattt
    33961 ccacttcttt ttatcctgtt cgatcttttg cggctgcacc tcaagaaata aactattcgt
    34021 tatcaggcgg taaagtttac gcagataagt tcagtatcgc cattggggtt gaggcaaaag
    34081 ctcaggggga taataccaaa cttagcaacc tcaatcggca catctgccga agcaaatgca
    34141 cccggggcat tggctttaag gggaaattct gaagcatcga aaaaatttag tatagcagaa
    34201 ggctatctgg catctagtga tggctatggt gcaatagcga ttggttctgc cgccaagata
    34261 aagcaattgg aaaaaggcac aataaatcat attgtgggaa acgataataa aggtctttat
    34321 gtcgatgcag acggcaatgt aacaaagata accgtaagga ctgagtcgga aaaagatatt
    34381 ctctcaagat acggtcaaac ttacggtgcg atggcactag ggtttagatc ttcttcacat
    34441 aatctttttg ccagttcatt tggagcgttt tctacagcca cagctattga aagcttggca
    34501 gtcggcgaca gcagccaatc aacgggctac cgcagtgcta cttttggcag tcacagcagg
    34561 gctttggcag aagaaagttt ggcattaggt tatgaaactc gggcaaatgc ttatggttcc
    34621 gttgctttag gtgcagaatc tgtggcgaat gaagaaaata ccgtatcagt gggttccgat
    34681 acattgaaac ggaaaatcgt taatgtcgcc gatggcacgg aagatttatg atgcagtaaa
    34741 tgtccgccag ttgaaccgct taagcaaacg tacaaaccgc gtcggcgcaa gtgctgcggc
    34801 gttggcttcg ttaaaacctg cacaattagg caaaaacgac aaattcgcct tttctttggg
    34861 ctttggaagc tataaaaatg cccaagcggt ggcaatgggg gcggtgttta agcccgctga
    34921 aaatgtgttg cttaatgtgg cgggcagttt tgccggaccg ggctttcggc gcgggggttt
    34981 cttggaaatt cggcggcaaa ccgacacctg cggttgccgc acaaaacgcg gcgcatcctg
    35041 caaaagtttt gcaattgcgg caggaagtgg cggcgttgcg cgcccggcag gcagaaaccg
    35101 accgcaaact gcacaaacag gccgaaatgg aaaacgagct gcaacagttg cgccgcgcgt
    35161 tgtccgaatt gaaaaaacat tgaagcaata aacgtaaggc gggacggcgg ctctgccttt
    35221 ttccgatgcc gcacccgcgc gatgccgtct gaagccttca gacggcattt ttaacgcgcc
    35281 cgaaccccgg ttccgccaat gcccgatgct tggcgcggca tttgccgtat tgccccgccg
    35341 ccgggcgcgt tataatccgc cacgacatcc gcccgatttg gaaacctgcc tatgcgaccc
    35401 tcctatttca tttccgacct gcatttgagc gaaaagcacc ccgaactgac cgaactgctg
    35461 ctgcgttttt tacgttctgc cgccgccggg caggcgcggg cggtttatat tttgggcgat
    35521 ttgtttgatt tttgggtggg cgatgacgaa gtttccgagt tgaatacttc ggttgcgcgg
    35581 gaaatcagga aattgtccga caaaggcgtt gccgtgttct ttgtcagggg caaccgcgat
    35641 ttcctaatcg gtcgggattt ttgccggcag gcgggtatga cgctgctgcc ggattactcg
    35701 gttttggact tgttcggcag caacaccctg atttgccacg gcgacacatt gtgcaccgac
    35761 gacaaggcat acctgcgttt ccgccgcatc gtgcattgca ggcggctgca aaaactgttc
    35821 ctgatgctgc ccctgaagtg gcgcacgcgc cttgccgcca aaatcaggcg tgtcagcaaa
    35881 atggaaaaac aggtcaagcc cgccgatatt atggatgtca atgccgcctt taccgcgcgg
    35941 caggttcgcg cctttaacgc ggaaaggctg atacacggac acacccaccg cgagcatatc
    36001 catcacgaaa acggctttac ccgcatcgtt ttgggcgact ggcataacga ctatgcttca
    36061 atcctccgcg tggacgggga cggcgcggta ttcgtgccgc cggaagaatg ctgaaaatgc
    36121 cgtctgaagc ctttcgggcg gctttttcta cacgcccctt tcccacactc cgtccgccgc
    36181 atcatcacca tccttaaccc gcattaatag ccattatcga taaaatgctt gaccgattac
    36241 ataagattac gtaaagtgtg caaacgcata ataattggtc ttaccaattt atcttttaac
    36301 caaaatatcg ggtgtgcggg atttgaacgt ctttccaaac ctcgacgcgc cttatgatgc
    36361 aaatgcctaa aaaggattcg ttatggcact tttcctcagc atattcccca tcgtcctgct
    36421 gatttggctg atggtgaaaa aaaacagtat gccttcctac gtcgcactgc cgattaccgc
    36481 agtgctgatt tacgccatca aacttttcta cttcggcgat gcgggaatgc tgctcaacgc
    36541 caccgccgct tccggcctcg tcaaaacgct cacgccgatt accgtgattt tcggcgcgat
    36601 tatgttcaac cgtatgatgg aaaccacggg ctgcatcgat gtcatccgca aatggctggc
    36661 gaccatcagc ccgaaccccg tagcgcaact gatgattatc ggctgggctt ttgcctttat
    36721 gattgaaggc gcatccggtt tcggcacgcc tgccgcgatt gccgcgccga ttctgatgag
    36781 cttgggcttc aatccgttga aagtggcgat tttcactttg gtgatgaatt ccgtgccggt
    36841 atcgttcggc gcggtcggta cgccgacctg gttcggtttc gcaccgctga acctgagtgc
    36901 cgaagacatc ctcgccatcg gcaggcagac cggcgtgatg cacttcttcg caggtttcgt
    36961 catccctgtc atcggtttgg gcttcatcgt gccttggtct gaaatccgca aaaacttggg
    37021 cttcgtcgcc attgccgtct tctcctgcac cattccttat gccgcattgg cgatggtcaa
    37081 cgaagaattc ccgtcgctcg tcgccggcgc aatcggcctg atggtgtccg tattcgccgc
    37141 caaccaaggc tggggcttga gcaaagacca cgccaaagac ccgaatgccg aaaaagtgcc
    37201 gttcgcccaa gtcgccaaag cactcgcccc tttgggtatg ctgatcggca tgctggtggt
    37261 tacccgcatc aaacagctcg gaatcaaagg cattttgacc agcaaagaag aatggttcag
    37321 cttccaactg ccgtttgatt tgtccaaaat taccgtcagc gactccctga cgattacctt
    37381 cggcaatatt ttcggacaag atgtcagcgc gtcttaccaa acgctgtacg tcccggcttg
    37441 gattccgttc gtgctgaccg tttggatttg catcctgctg tataaaacca aattcaaaga
    37501 tgcctggacg atttatgccg taaccttcaa tcaaaccaaa aaaccgctgc ttgccctgat
    37561 gggtgcgctg attatggttc agctgatgct ggtcggcggc gacaattcga tggtgaaaat
    37621 catcggtaag gaatttgccg caatggcggg cgaacactgg gtttattttt cgccgtatct
    37681 gggcgcgatc ggtgcgttct tctccggctc caacaccgtg tccaacctga ccttcggtcc
    37741 gattcagcag caaatcgccc ttgataccgg cctgtccgtg accctgattc tggcgttgca
    37801 gtccgtcggc ggcgcgatgg gcaatatggt gtgcctcaac aacatcatcg ccgtatgtac
    37861 cgtattggac gtgaaaaatt ccgaaggtgc gattatcaag aaaaccgtta tcccgatggc
    37921 gatttacggc gtgattgccg tcgtcgcggc aatgattttc ttcctctagg cgaaacgccg
    37981 ccatccatag cggcagcacg cggggaacgg aaaccgccgc tcgtccgggc ggcgcgtcgg
    38041 gcagaatacc cgtgccggaa cagcccgccc cgccaaacaa atgccgtctg aaaccggaaa
    38101 aggcttcaga cggcattttt atcgccgttt gcattcaggc gtagtaatga tgcggcatct
    38161 tgccgtcaag gcggtagcgc gtgccgcaat acgggcaggc aacgctgccc gattctcctt
    38221 cgcccaaagg taaaaacacc ctcggatgcc cgttccactg ctcgttgtcc ggtcccgagc
    38281 aatacagcgg cagattttcc ggcaacacgg aaatttcctg cggattcaga ttgtccattt
    38341 gattttcctt tgcgtggttg gcgtgcgccc attttacgcc atcggcaggc taaaggatat
    38401 tttcggcgca aagccgcaat ccgctataat ccccactttt cagacggcat accatgactg
    38461 cgcttaccct tcctgaagac atccgccaac aagaaccatc cgttctgctc tataccctcg
    38521 tttccgccta cctcgaacac accgcccaaa ccggcgacga atccctctcc tgcctgtccg
    38581 acgaccagca cacgctgacc gcattctgct acctcgacag ccaagtcgaa gaaggcggct
    38641 tcgtacagct tatcgcctcc ggctacggcg aatacatttt ccgcaacccg cttgccgaca
    38701 gcctgcgccg ctggaaaatc aaagccgtgc cgaaagtctt ggacaaagcc aaagccctct
    38761 acgaacaaca cggcgaaacc atcgaaacgc tcgccgacgg aggcgcagac atcgaatccc
    38821 tgcgcaaaca gttccccgac tttgaagaat gggacggcgc atactacgaa gccgccgaac
    38881 aagacctgcc cctgcttgca gaacacatcc tgtcaaaccg ggcggcattc gcccatatcg
    38941 ggcaggcgtg attgcgtctg tttccagccc gtgtaaaaca gcgtaaaatc ggcaaacccg
    39001 aattatcttc cgtccccatc aaggagcaag ctatgttctt caagcacatc gaagccgccc
    39061 ccgccgatcc gattctcggt ttgggcgaag cgttcaaagc cgaaacccgc cccgaaaaag
    39121 tcaacctcgg catcggcgtg tacaaagacg catccggcgc gacacccatt gtcaaagccg
    39181 tcaaagaggc tgaaaaacgc ctgttggaaa gcgaaaccac caaaaattac ctgaccatcg
    39241 acggcgttgc cgactacaac gagcaaaccc aaatcctgct gttcggcaaa gaccacgaaa
    39301 tcatcgccag ccgccgcgcc aaaacagcgc aaagccttgg cggtacaggc gcattgcgta
    39361 ttgcggccga atttgccaaa cgccagttga acgcgcaaac catctggatt tccaatccga
    39421 cctggccaaa ccacaacgcc attgccaaag cggtcggtat ccaagacaaa ccttatcgct
    39481 actatgatgc cgccaaacac tgcttggatt gggacggcat gattgaagat ttgaaccaag
    39541 cgcaaaaagg cgacatcgtc ctgctgcacg gctgctgcca caaccctacc ggtatcgacc
    39601 ctacgcccga acaatgggaa actctggcaa aactttctgc cgaaaaaggc tggctgccat
    39661 tgtttgactt tgcctaccaa ggctttggca acggtttgga agaagatgcc tatggcttgc
    39721 gcgtgttctt gaaacacaat acagaattgc tgattgccag ctcttattcc aaaaacttcg
    39781 gcatgtacaa cgagcgcgtc ggcgcgttca ctttggtggc cgaagatgaa gaaacagcag
    39841 cccgcgccca cagccaagtc aaaaccatca tccgtacctt gtattccaac ccggcttcac
    39901 atggtgcgaa caccattgca ctggtgttga aaaatgatga tttgaaagca caatggattg
    39961 ccgaactcga tgaaatgcgc ggccgcatca aagccatgcg ccaaaaattt gtcgagttgc
    40021 tcaaggccaa aggttcaacc caagactttg attttattat tgaacaaaac ggtatgttct
    40081 ctttcagcgg cttgactccc gaacaagttg accgtttgaa aaacgagttt gccatttatg
    40141 ccgtccgttc cggccgcatc aacgtcgccg gcattaccga caacaacatc gattatctgt
    40201 gtgaaagcat cgtgaaa
//
LOCUS       DAXDXW010000008        39990 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXDXW010000008 DAXDXW010000000
VERSION     DAXDXW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39990
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.008"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..137
                     /locus_tag="ACFB64_000345"
     CDS             <1..137
                     /locus_tag="ACFB64_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Imm41 family immunity protein"
                     /protein_id="HFC1208790.1"
                     /translation="IKSSPWLPDSVGIHERYERFTTMLRYIFTEKDIVNVRFDYYNKK
                     "
     gene            complement(201..2414)
                     /locus_tag="ACFB64_000346"
     CDS             complement(201..2414)
                     /locus_tag="ACFB64_000346"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC1208791.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKNLIGTAWSLA
                     QEHYPADAATPYGEPLPDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLDILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSGKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSATTIVKQSKIKKGGKTGVLIDGEDGLMTTLAKCCKPAPPDDIAGFVTR
                     ERGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLAGLGDVKGVLSVTRL"
     gene            2557..3198
                     /locus_tag="ACFB64_000347"
     CDS             2557..3198
                     /locus_tag="ACFB64_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC1208792.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEVVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVCHFDREGRLKDEVRPEIQAWLDKVGGYNNKLVQPRL
                     IKIGLPEFVTPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGTSD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
     gene            complement(3270..3908)
                     /locus_tag="ACFB64_000348"
     CDS             complement(3270..3908)
                     /locus_tag="ACFB64_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC1208793.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCIAASGP
                     LSNLAMAVLWGVVLVLTPYAGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLPAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            4164..5015
                     /locus_tag="ACFB64_000349"
     CDS             4164..5015
                     /locus_tag="ACFB64_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC1208794.1"
                     /translation="MDKERILTPAVVFSVALLHLAIVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGGGAPEGAGAPAAPEPQPAPDPPKPVEPPKPVLKPAVTKKADADIQQPKEK
                     PKPEEKPKPEPEPEAKPAPKPAEKPAEKPSEKPAEHSGNASAKAGSEQGNGEGKGTGT
                     KGDGTGRGEGSGKGSGGAKGEHGEGAGGSGGGTGVGSSKGNPLRANGSIPRPAYPALS
                     MENDEQGMVVLSVLVSPGGHVESVKVVKSSGFSRLDNAARKAAQNGHFQANAWTEFKV
                     PVKFELN"
     gene            5081..5743
                     /gene="exbB"
                     /locus_tag="ACFB64_000350"
     CDS             5081..5743
                     /gene="exbB"
                     /locus_tag="ACFB64_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC1208795.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSTVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            5746..6180
                     /locus_tag="ACFB64_000351"
     CDS             5746..6180
                     /locus_tag="ACFB64_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC1208796.1"
                     /translation="MAFGSMNSSDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQANKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(6681..7109)
                     /locus_tag="ACFB64_000352"
     CDS             complement(6681..7109)
                     /locus_tag="ACFB64_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC1208797.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(7174..8922)
                     /locus_tag="ACFB64_000353"
     CDS             complement(7174..8922)
                     /locus_tag="ACFB64_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC1208798.1"
                     /translation="MIHYKIAPSPLDHEWHILLKFTQDNDFPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHTAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPPEWRIATTLPETGRLVFQTASYDELIDRP
                     VEMGLIEFLDFEAAGIPHTIALNGIYPDFDRDRLVSDIKKICETELAMFSSPAPFEKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPEFYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTVMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLTDFFQTALYSTEDLPLAEC
                     LATTGVKLTFLPLPRQHGGGYAEHIFPIPPTGDFGARFKQNADHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFAAQWARYHVRAKINIHFFRAGILRQTVLTVQATAADTA
                     ILHITDRNLLENWLFG"
     gene            9248..10681
                     /gene="ccoN"
                     /locus_tag="ACFB64_000354"
     CDS             9248..10681
                     /gene="ccoN"
                     /locus_tag="ACFB64_000354"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC1208799.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKVKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYMIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            10708..11319
                     /gene="ccoO"
                     /locus_tag="ACFB64_000355"
     CDS             10708..11319
                     /gene="ccoO"
                     /locus_tag="ACFB64_000355"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC1208800.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            11324..11494
                     /locus_tag="ACFB64_000356"
     CDS             11324..11494
                     /locus_tag="ACFB64_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC1208801.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDDAANSIFAE
                     NQDAQDKKSENR"
     gene            11518..12837
                     /gene="ccoP"
                     /locus_tag="ACFB64_000357"
     CDS             11518..12837
                     /gene="ccoP"
                     /locus_tag="ACFB64_000357"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HFC1208802.1"
                     /translation="MNATSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPKGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSNKDGKAPVKKAEPAPAAEPAPSAPAEAAQ
                     AASEAKPAAAEPKAEEKAAPAAKADGKQVYETVCAACHGNAIPGIPHVGTKADWADRI
                     KKGKDTLHKHAIEGFNTMPAKGGRGDLSDDEVKAAVDYMVNQSGGKF"
     gene            13181..14590
                     /locus_tag="ACFB64_000358"
     CDS             13181..14590
                     /locus_tag="ACFB64_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC1208803.1"
                     /translation="MDTQIKTEAGQQNNRHYLSVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVFPQAVVQPLSVQAEAARGAINPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVNTMPRNQGWYHTMDEIHGDMMLGAAGDYLLETAASL
                     TIIMVVSGLYLWWAKQRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGENGINPSEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGVAGIVPPAQKIKLPVWWAMALPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRKWFK"
     gene            14691..17900
                     /gene="recC"
                     /locus_tag="ACFB64_000359"
     CDS             14691..17900
                     /gene="recC"
                     /locus_tag="ACFB64_000359"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC1208804.1"
                     /translation="MFYLYQSNRLESLAALFARIQKVKPLKCALQPEQIVVQSQGMRR
                     YLNTCLARDLGVAANLAFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSA
                     EFQNGAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMHWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIEPYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYALECVSDLLESRFEVGKVLALLETAPVLRRFGLTGDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAADRMILGWMLPEGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLSDIARIWRQPATVGEWVARCRDLLETLFQAGPDDQKAVQNLE
                     NEWVKWQEESTLAGFFGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLVAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPRPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHAEQIAETYIGARREGQDFSQTAARIAAESLLPSG
                     ELGKLWQQSFQTAAKQIDTAVLNSPKLPPFPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVKSGETEVLAEIAQDRALQLLSEWI
                     VFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGSKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGTGEA"
     gene            complement(17970..19538)
                     /gene="mtrF"
                     /locus_tag="ACFB64_000360"
     CDS             complement(17970..19538)
                     /gene="mtrF"
                     /locus_tag="ACFB64_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFC1208805.1"
                     /translation="MSQTDARRSGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAVI
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTLDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMAASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFIALSALLAWSIVPADGILRHPETGLVAGSPFLKSIVVFIFLLFALPGIVYGRIT
                     RSLRGEREVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGAVFLKEVG
                     LAGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPVP"
     gene            19870..20082
                     /locus_tag="ACFB64_000361"
     CDS             19870..20082
                     /locus_tag="ACFB64_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208806.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENIHAMIGEIKNAI"
     gene            complement(20175..20807)
                     /gene="mtrR"
                     /locus_tag="ACFB64_000362"
     CDS             complement(20175..20807)
                     /gene="mtrR"
                     /locus_tag="ACFB64_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFC1208807.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAADAEGGSWTVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSGESFDLGKTAPRIIGIMMDNLENHPCLRRK"
     gene            21057..22295
                     /gene="mtrC"
                     /locus_tag="ACFB64_000363"
     CDS             21057..22295
                     /gene="mtrC"
                     /locus_tag="ACFB64_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC1208808.1"
                     /translation="MAFYASKAMRAAALAAAVALALSSCGKGRDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVSADAISKQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGAIAVGIKFDDGTVYPEKGRLLFADPTVDE
                     STGQITLRAAVSNDQNILMPGLYVRVLMDQVAADNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGMTGAKKVTPKEWAPSENQA
                     AAPQAGVQTASEAKPASEAK"
     gene            22307..25510
                     /gene="mtrD"
                     /locus_tag="ACFB64_000364"
     CDS             22307..25510
                     /gene="mtrD"
                     /locus_tag="ACFB64_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC1208809.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHAIYPGASAQVMEGSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNV
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADVGSALSAQNIQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNIYLKDVAKVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERLAVLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMTLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGAAGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFDSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATKERTDATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTASGSDAVAVAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAAGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVRHASKAGITGSDDKQY"
     gene            25563..26966
                     /gene="mtrE"
                     /locus_tag="ACFB64_000365"
     CDS             25563..26966
                     /gene="mtrE"
                     /locus_tag="ACFB64_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC1208810.1"
                     /translation="MNTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFQNDTSV
                     SSIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYNVGLGAASYELDLFGRVRSSSEAALQGYFASVANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINRPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVGTGSVELGGLFKSGTGVWA
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERISYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTGK"
     gene            complement(27167..27359)
                     /locus_tag="ACFB64_000366"
                     /pseudo
     CDS             complement(27167..27359)
                     /locus_tag="ACFB64_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27365..27571)
                     /locus_tag="ACFB64_000367"
                     /pseudo
     CDS             complement(27365..27571)
                     /locus_tag="ACFB64_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            27921..28700
                     /locus_tag="ACFB64_000368"
     CDS             27921..28700
                     /locus_tag="ACFB64_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC1208811.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNILVARQILEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIRFWLEKFEEACGSGNLEHQGEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            complement(28738..28844)
                     /locus_tag="ACFB64_000369"
                     /pseudo
     CDS             complement(28738..28844)
                     /locus_tag="ACFB64_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(28939..30273)
                     /gene="gdhA"
                     /locus_tag="ACFB64_000370"
     CDS             complement(28939..30273)
                     /gene="gdhA"
                     /locus_tag="ACFB64_000370"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC1208812.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFTSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSDGFVLFPDIGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCHVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWA
                     REEVDSRLFGIMQSIHESCLKYGKVGDKVNYVNGANIAGFVKVADAMLAQGF"
     gene            30697..31491
                     /locus_tag="ACFB64_000371"
     CDS             30697..31491
                     /locus_tag="ACFB64_000371"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC1208813.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRSWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAVVSV"
     gene            31500..32514
                     /locus_tag="ACFB64_000372"
                     /pseudo
     CDS             31500..32514
                     /locus_tag="ACFB64_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703756.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
     gene            complement(32689..34026)
                     /locus_tag="ACFB64_000373"
     CDS             complement(32689..34026)
                     /locus_tag="ACFB64_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC1208814.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAVRSLTLPDAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PNQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGAWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLGIL"
     gene            complement(34260..35324)
                     /locus_tag="ACFB64_000374"
     CDS             complement(34260..35324)
                     /locus_tag="ACFB64_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC1208815.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     DELMRRRFSEVSSVFTTRLHTFNGLFSLYALTRFIRENHISHLMIHTGKIAALSVLLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRGVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVAARFTINHTADKLLDAI"
     gene            complement(35325..36083)
                     /locus_tag="ACFB64_000375"
     CDS             complement(35325..36083)
                     /locus_tag="ACFB64_000375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC1208816.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSADNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVRTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKGGSVQGKVHETVQTPYPERRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVSFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(36158..36233)
                     /locus_tag="ACFB64_000376"
     tRNA            complement(36158..36233)
                     /locus_tag="ACFB64_000376"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36198..36200),aa:Ile,seq:cat)
     gene            complement(36287..37632)
                     /locus_tag="ACFB64_000377"
                     /pseudo
     CDS             complement(36287..37632)
                     /locus_tag="ACFB64_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757555.1,RefSeq:WP_003777802.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase"
     gene            complement(37626..38355)
                     /gene="fabG"
                     /locus_tag="ACFB64_000378"
                     /pseudo
     CDS             complement(37626..38355)
                     /gene="fabG"
                     /locus_tag="ACFB64_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
     gene            complement(38393..38821)
                     /locus_tag="ACFB64_000379"
                     /pseudo
     CDS             complement(38393..38821)
                     /locus_tag="ACFB64_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677797.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioester dehydrase"
     gene            39021..39764
                     /locus_tag="ACFB64_000380"
                     /pseudo
     CDS             39021..39764
                     /locus_tag="ACFB64_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            39767..39850
                     /locus_tag="ACFB64_000381"
                     /pseudo
     CDS             39767..39850
                     /locus_tag="ACFB64_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
BASE COUNT         9323 a         9850 c        11190 g         9627 t
ORIGIN      
        1 caattaaatc ttccccttgg ttgcctgata gtgtgggaat tcatgaacgt tatgaaagat
       61 tcacaacgat gctccgttat atttttaccg agaaagacat agtcaacgtg cgatttgatt
      121 attacaacaa aaaatagcaa aacccggcaa tcaaaatgcc gtctgaagcg ttatccggct
      181 ttcagacggc atttttgtat ttaaagccgg gtaacgctca atacgccttt gacatcgccg
      241 aggccggcga ggacgcgcgg gaggtcgttg acttgtttga cttcgagcgt gaacctcatg
      301 ctggcttcca agtcgcggga ctgggtttgt acggcggtaa cgttgagttt gtggcgggcg
      361 agcgcgtcgg atacgtcgcg caaaagcccg gagcggtctt gggcgcggat ttcgatatcg
      421 acggcgaaca cttgcccttc ctgcaacgcc gcccaacttg cgtccagtac tttttcgggc
      481 gcgtgttcgg caaggtgtcg gaaagagggg caggttttgc ggtggacgga aatgccgcgc
      541 tcgcgggtaa cgaagccggc aatatcgtcg ggcggcgcgg gtttgcagca tttggcaagc
      601 gtggtcatca agccgtcttc gccgtcgatg agcacgccgg ttttgccacc ttttttgatt
      661 ttggactgtt tgacgatggt ggttgcgctg acgggcacgg gcggcggttc gttcagcgtg
      721 ccgcaggctt tttggatggc gcggttggaa atttcgcctt gtccgacggc ggtgtagagg
      781 tcttctggct ttttgtagcc gagattttcg gcaagctctt gcaggttggg tttgggcgtg
      841 agtttggcaa gctgcttgtc gagttggaca cggccttctt cgcgcacggt gtcggcgttt
      901 tgctggcgga tgtaggcgcg gattttgccg atggccttgc cggatttgac ccagccttcg
      961 taaagccagt tgacggaagg atgcccttct ttggcggtaa tgatttcgac gcgctgtccg
     1021 ttttcgagcg gggtggacag cggcacaatc tgcccttcga ctttcgcgcc ccggcagcgg
     1081 tcgccgatgc tgctgtgcag ggcgtaggcg aagtcgatgg gggttgcgcc cgttggcaga
     1141 gagaggactt tgccgtgcgg ggtcaaaaca taaatcgtgt cgttgaaaag ctcggttttg
     1201 aaggcggcgg cgaggtcttc cttgccgctt tccgccatat tttcgcgcca gtccaagagt
     1261 tggcgcaacc aggcgatttt ttgttcgtag gcggaatcgc ctttgccgcc ttctttgtaa
     1321 cgccagtggg cggcgacacc gaattcgttg aattggtgca tatcgaaggt gcggatttgc
     1381 acttccacac ctttgtcttc cgggccgacg atgacggtgt gcaaactttt ataaccgttg
     1441 cctttggggt tggcgatgta gtcgtcgaac tcgccgggaa tgggctgcca gaggctgtgg
     1501 acgatgccca gcgtggtgta acactcgggg acggtatcga ccagaatccg cacggcgcgg
     1561 atgtcgaaca ggccgtcgaa gctgagtttt ttcttcacca tttttttgta aatggagtag
     1621 atgtgtttcg gacggccggc gacttcaaag tggatattgt attttttgag ttccgtacgc
     1681 aggatatcga ggaagttttc gatgtattcg aggcgttcgg tgcgtttttc gtccaaaagc
     1741 agggcgattt cgcggtattt ttcgggttct tgatggcgga agcccaaatc ttcgagctgc
     1801 catttgagct gccacacgcc caagcggttg gcgagcgggg cgaagatgtc gagggtttct
     1861 ttggcgacgg cgcgtttttc ggggctgtcg ggggcgttgc ttaaaaattg cagggtgcgc
     1921 gtacgcatcg ccagtttgat taatacgacg cggatgtcgg taaccatcgc cagcagcatt
     1981 ttccgcatgg tttccgcttg ctgtgcgcgt tcttccggcg tggcgaggct gtccacccgg
     2041 gcgaagtggg taagtttctg cacttcgtcc acacctttga ccagctcggc gacggtgctg
     2101 ttgcagcgct cggaaaccaa taggttccaa tcggggacgt agcgtccgat gtcggcaagc
     2161 agggtggcgg cgacggcatc gggcagcagg tcgagttcgt cgaccatttg cgccgcgccg
     2221 aggaagtggt cgggcagcgg ctcgccatac ggcgtggcgg catcggcagg ataatgttcc
     2281 tgcgccagcg accatgcggt accgatgagg tttttatcgt tgtccggcag agcggcgcag
     2341 tagctgtcga accattcgcg caattcttgc agggtcgcgc tttgcgtgtc ttgaatcggg
     2401 ctgatggcgg tcatgatggg atcttccgtt ccgataaaaa cgagttcatt gtaacagctt
     2461 atttatttac ggtgcatagg cgggaagata aacttgtttc gggcggcgcc gggtgttaag
     2521 gtagccggta ccgacaggtt gacgaggaat attttgatga aactgtatat ttacgaccat
     2581 tgcccgtttt gcgtgcgtgc gcggatggct gccgggctgt ttggtgctga cgttgaagaa
     2641 gtcgtgctgg caaacgacga cgaggcaacg ccgatcggta tgatcggtgc gaaacaagtg
     2701 cccgtcctgc aaaaagaaga cggttcgttt atgggcgaga gtttggacat tgtgtgccat
     2761 ttcgaccgag aaggccgtct gaaagacgaa gtccgccccg agatacaggc gtggctggac
     2821 aaggtaggcg gatacaacaa caaattggtt cagccacgct tgatcaaaat cgggctgccc
     2881 gaatttgtaa cgccggaagc ggtgaaatat ttcacagaca aaaaagagaa aagcatcggc
     2941 agcttttccg ccaatttgaa taaaaccgcc caataccttg aacggataaa cgcagatttg
     3001 caagagttgg agaatttgat ggacggtact tcagacggca tcaacggtgg aatcggtatg
     3061 gaggatattt tggttttccc cgttttgagg aatctgaccg tggtgcgcgg catcgcgttc
     3121 cctcggaaga cgatggatta tttaatcggt atgagtgaaa aatcaggcgt gccgctttat
     3181 ttcgacaggg cattgtaaaa ccaaaaaaaa gcggcagggc aatatgtcct tccgcgtttt
     3241 ttggaacgtt tatgccgtct gagagccagt cagacgaaca tctgcacaaa cgcaatcacc
     3301 agccgcacaa tcggtgcaat aaacgcaccc aaaaccccgg tcagcataag cagcaggata
     3361 atccacgtcc cataaggttc gattttgcgg aacgcttgcg aatatttcgc cggcaggaag
     3421 gtgtcgatga aaatgccgcc gtcccaaggc aggatgggga tgatgttgag cgcgaacaaa
     3481 atcgcattga tcagaatacc gtagtttgcc atttgagcca gcggcatctg atacgccccg
     3541 ccggcatacg gagttagcac caaaaccacg ccccacagaa cagccatcgc cagattcgac
     3601 agcgggccgg acgcggcaat gcaacgccat gcaaggcgcg ggttgcggaa gttgcgcgaa
     3661 tcgataggaa tcggacgcgc ccagccgaac aggaagggcg tgaacatcaa agtaagcagc
     3721 ggtacgatga ttgtgccgac caaatcgata tggggcaggg ggttcagtgt cagcctgccg
     3781 tattgttcgg cagtgttgtc tccccagtag cgcgccgtat agccgcgcgc cacctccctg
     3841 acggtaatgg cgagcagcac gggcaggaca gcaagcagaa acacgcccaa gtcaaaattt
     3901 tgaaacatgg ttttcctttg aacagatgtg gtgtgcagcc cgaaacggct gacagacaga
     3961 agcggcaata tatcatctaa cctgatttta ttggaataag aaatcagacg gaatcatttt
     4021 ttctgccggc ccgttatctg atgatttgct gtaataccgc gtttaaacac ggtttgccta
     4081 tgaatggagc aggcgttcac atgacaacaa attataaaaa ataggttgca aataggaatt
     4141 gttgctatta ttatgcccga attatggata aagaacgaat tttaaccccc gcagtcgtgt
     4201 tttccgtcgc actgctgcat ttggcaatag tggcattgct ctggcaggcg cacaagctgc
     4261 ccgtgataga gtcaggaaat gttatcgaat ttgtcgattt gggcgatttt ggcggagggg
     4321 gcggcgcacc cgaaggcgca ggcgcgcctg ccgcgcccga accgcaaccc gcgcccgatc
     4381 cgcccaaacc tgtcgaaccg cccaagccgg ttttgaagcc ggcggttaca aaaaaggcgg
     4441 atgcggatat tcagcaacct aaggaaaagc cgaaacccga agaaaaacct aagcccgaac
     4501 ccgaaccgga agcgaagcct gccccaaaac cggcggaaaa accggcagag aagccgtctg
     4561 aaaaacctgc cgaacattcc ggtaatgctt ctgccaaagc cggcagcgag cagggtaatg
     4621 gggaaggtaa gggaaccggt accaaaggcg acggaacggg gcgcggagaa ggcagcggta
     4681 aaggaagcgg cggtgccaaa ggcgaacacg gggaaggagc cggcggcagc ggcggcggaa
     4741 ccggtgtcgg cagcagcaaa ggcaatcctt tacgcgccaa cggcagcatc ccgcgcccgg
     4801 cctatcccgc actttctatg gaaaatgacg agcagggtat ggttgttttg agcgttttgg
     4861 tttctccggg cggtcatgtc gagtccgtca aagtggtgaa aagcagcggt ttttcccgtt
     4921 tggacaatgc ggcacgcaaa gcggcgcaaa acgggcattt tcaagccaat gcctggacgg
     4981 agtttaaagt ccccgtcaag tttgaattga attaggcggt tgtcgcggca gtttgcggca
     5041 gcttggatgt ttgattggtt ttttcagtgt tggaatcatt atgaatttga aattagtgtt
     5101 tgaatcgggc gatcccgtcc tgatcggtgt gtttgtgttg atgctgttga tgagtaccgt
     5161 aacgtggtgt ctggttgtct tgcgctgcat caagctgtat cgggcgcgca aagggaatgc
     5221 cgccgtcaaa cggcatatgc gcgatacttt gtcgctgaac gacgcggtcg aaaaagtgcg
     5281 cgccgtcgat gcgcctttgt ccaaactggc gcaagaggca ttgcagtctt accgcaacta
     5341 ccgccgaaac gaagcgtccg aactggcgca ggctttgccg ttgaacgagt atttggtcat
     5401 tcaaatccgc aacagtatgg cgcagattat gcgccggttt gattacggga tgaccgcgct
     5461 tgcctccatc ggcgcgaccg cgccgtttat cgggttgttc ggcacggttt gggggattta
     5521 ccacgccctg atcaatatcg ggcaaagcgg gcagatgagt attgcggcgg ttgccggccc
     5581 gattggcgag gcactggtgg cgacggcggc gggtttgttc gtggcgattc cggcggtgtt
     5641 ggcatacaac ttcctcaatc gcggcacaaa aatactgacc caggatttgg atgcgatggc
     5701 gcacgatttg cacgtccgcc tgcttaatca aaaggatagc tgattatggc atttggttcg
     5761 atgaattcca gcgacgattc tccgatgtcc gacatcaacg ttacgccgtt ggtggacgtg
     5821 atgctggtgt tgctgattgt gtttatgatt actatgccgg tgctgacgca ttccatccct
     5881 ttggaactgc cgaccgcgtc cgagcaggca aacaagcagg acaaacagcc taaagacccc
     5941 ctgcgcctga cgattgatgc gaacggcggc tattatgtcg gcggggattc tgcaagcaaa
     6001 gtggaaatcg gggaagtgga aagccgtctg aaagccgcca aggagcagaa tgaaaacgtg
     6061 attgtggcga ttgcggcaga caaggcggtg gaatacgatt atgtaaacaa agctttagaa
     6121 gccgcccgtc aggcaggaat taccaaaatc ggttttgtaa ccgaaaccaa ggcgcaataa
     6181 agttcgacgc ttgaacgaat gccgcctgaa agtctttcag acggcatttc ttatgtttaa
     6241 tcagttatat atagttgatt aaaacaaaaa tgagacgaca gagcaccgtc tccatttctg
     6301 cgcaggcggg aatacaaaca tctgattttt cttttatttt caaacattac tgaaatgggt
     6361 aagtctggag tcctgcctgc gcgagaataa tgaaaatgtg catttctaat ttttatagtg
     6421 gattaacaaa aatcaggaca aggcaacgaa gccgtagaca gtacaaatag tacggaaccg
     6481 attcacttgg tgcttcagca ccttagagaa tcgttctctt tgagttaagg cgaggcaacg
     6541 ccgtactggt ttttgttaat ccgctatacc cactgtaaaa acggagaagg acagaggacg
     6601 gcagtaaggt ttgccgtcct ctttttcaag ttgcggtgga atgccgcttg aaggtgtggt
     6661 ttaaacgttt cggcttgtaa tcagacgcgg gcgttgtcca gccgctctct gaagttttcc
     6721 agattcacac ctgccgcttg ggcgcggcgg atgatttcgt caaccggcat accttctgcc
     6781 gcccggcgga agccccacag gagggagcag cgcgtaccgg tccggcaata ggcaaggacg
     6841 ggatattcgg cttgtccgat gagttggcgg aaggtttcga catcgtgttt ttggatgtcg
     6901 cgtgcggtaa cgggttggtg atggaatccg gtaacgcctg cttgttccag ccactgtttg
     6961 atttgggcga agtcgggttg cgattcttct tcgcggtcgg ggcggttgca gatgacggtt
     7021 ttgatgccaa gttgcgcgat ttgttccgca tcggctttgg tcagttgcgg ggagatgtag
     7081 agatgttcgt caagtttgag aatcgccatt ttgtttcctt tgctttgcgg ttggtgttca
     7141 gacggcattt tgtgtgcaat gccgtctgaa agtttaaccg aacaaccagt tttccaacag
     7201 gttgcgatct gtgatgtgta ggatggcagt atccgccgcc gttgcctgaa ccgtcaagac
     7261 ggtttgacgc aatatgcctg cacggaagaa gtggatattg atttttgccc tgacgtggta
     7321 tcgggcccat tgtgcggcaa agtcggtgca ggcataaccg tctaaagcaa tgattttgtc
     7381 ttgcgggcac agtgccgcag attccgcact tccgccgttg aagacatggg tcaggacgat
     7441 atggtcggcg ttttgtttga agcgtgcgcc gaaatcgccc gtcggcggga tggggaaaat
     7501 gtgttccgca tatccgccgc cgtgttgtcg gggaagcggc aggaaggtca gtttcacgcc
     7561 tgtggttgcc aggcattcgg caagcggcaa atcttcggtg ctgtacaatg ccgtctgaaa
     7621 aaaatctgtc aaatccaagc cggtaatttc ctgacaacgg atttgccagt gtttttccgg
     7681 aatacccgag tgtgtgtccc tccactcccg atagagtttg tccattaccg tatcaaggga
     7741 atgtctgccg ttgcttcggt tgcgtattat cagatcaagg cacaatgcgg caagtgcgcc
     7801 tttttggtag tagctgacga tggcgttggg gctgttttca tccggtttgt aaaatttgtt
     7861 ccacgcggta aaactcgatt ccgccaaggt ctgcctcaaa cggccgcggg tttgttgtac
     7921 gcgcgtaatg ccttgtgcta gcaggtttaa ataaaattcg ggcgagatgg tgcggctgcg
     7981 tgccaaaaac aaatcgtcgt aataggatgt aatgccttcg aatgcccata gttgttcggt
     8041 atagttttct ttgtcgaggt cataaggggc gaatgcggca ggtttgatgg atttgacgtt
     8101 ccacgcgtga aaatattcgt gggagaaaag tccgagcaat gtggtgtagg catcgtcggc
     8161 atcggtcata ccgtacggcg gaaggctgtg gcgatcggcg agcagtgcgg tgctgtcggt
     8221 gtgttccaaa ccgccgtaaa tatggtcgcc gacgtggagc aggaacaaat atttctcaaa
     8281 tggggcaggg gaggagaaca tcgccagttc tgtttcgcag atttttttga tgtccgaaac
     8341 cagcctgtcg cggtcgaaat cgggatatat gccgtttaag gcaatcgtgt gcggaatgcc
     8401 tgccgcctca aaatctaaaa attcaatcaa gcccatctcg acaggtcggt caatcaattc
     8461 gtcataagat gccgtctgaa agacaagcct ccctgtttcc ggcagcgttg tggcaatacg
     8521 ccattcgggt ggaatgcctg tcaattccaa gcggtgcggc agcgtttccg ttccttcgac
     8581 tttcaaaaac aggcacgatc cgtcaaaaaa accgcgttct gtcgtcagga aagaacctcg
     8641 aaccgacaaa tcgaatgcat atacggtgta gcggatttgc cactcgccgc gtacggcggc
     8701 agtgtgccag cggttttttg caatttgttc gagcggcatg gacgtgccgt tgcaggatgc
     8761 atggatagaa gtgatgtggc gggaaaaatc ccgaatcaga tagctgcccg gcacccaatt
     8821 cggaaggctt atttctatag gaaaatcatt atcttgtgtg aatttcagca ggatgtgcca
     8881 ttcatggtct agaggggacg gggctatttt ataatgaatc ataactatca aatatatcgt
     8941 taaacgggat ggattaaatg cctttaaaat gcagcttaac ctacatcaat aaagtgccac
     9001 aaaaagggaa aattggaaac aaaagcaatg gcggccgggt tgtaatttat tataacaata
     9061 tgatttatat ttaataaaaa acagattaag caaagggttc gccggcggcg cgaatgtaat
     9121 tccgcttggc gtaataaggc agatatgtta aatttagaaa aaattacgct tgcccggatt
     9181 gcaccttaaa caggctgcgg aagcggcagg ataaaaatct ttattcccat aattcatgga
     9241 gactctcatg gacacacaaa cttacaacta caaagtggtg cgccagttcg ccatcatgac
     9301 tgtagtttgg gggattgtgg gcatgttggt cggcgttatc gtcgccgccc agctttttgc
     9361 tcctgccctc gacttgtcta atatcggacc ttggttccac ttcggccgcc tgcgtccgct
     9421 gcacaccaat gcggttattt tcgcatttgg cggttgcggt ctgatcggca catcatacta
     9481 cgttgtccaa cgtacttgta atacccgtct tttcggcggt tggctgccgg catttacctt
     9541 ttggggttgg caggcggtaa tcgttgccgc cgtcgtcagc ttccctatgg gttggaccca
     9601 aggtaaggaa tatgccgaac tggaatggcc gattgacatt ctgattactt tggtttgggt
     9661 ggcttacgcc atcgtattct tcggtacgat tgccaaacgc aaggtaaaac acatttacgt
     9721 tgccaactgg ttctacggcg gctttatttt ggcggtcgca cttttgcaca tcgtcaacaa
     9781 tatcagcatc ccggccggtt tgatgaagtc ataccccgtc tattccggtg cgattgatgc
     9841 tatggttcaa tggtggtacg ggcataacgc cgtgggcttc ttcctgactg ccggcttctt
     9901 gggtatgatg tactatttcg tacccaaaca agcagcccgc cccgtttact cctaccgcct
     9961 gtccgtcgtt cacttctggg cgttgatttt cacctatatg tgggcgggtc cgcaccatct
    10021 tcactacact gcgctgcctg actggacgca atctttgggt atggttctgt ccttgatttt
    10081 gttcgcaccc tcttggggcg gtatgattaa cggcattatg accttgtccg gcgcgtggga
    10141 caaactgcgt acagacccga ttcttaaatt cctgattgta tccttgtcct tctacggtat
    10201 gtctaccttt gaaggcccga tgatgtcgat taaaacggtc aatgcattga gccactatac
    10261 ggactggacc gtcgcgcacg ttcatgcggg tgcgttgggc tgggtaggct ttgtaaccat
    10321 cggttccgtc tattacatga ttccccgtct gttcggcaaa gagcagatgc acagcaccaa
    10381 gctggtagag gcgcatttct ggattgcgac tatcggtgtg gttctgtata tcgccgcgat
    10441 gtggattgca ggtgtgatgc agggtttgat gtggagttct ctgaacgatg acggtacgct
    10501 gacctactcg tttgtcgaat ccgtaaaacg caccatgcct tactacatga tccgttttgc
    10561 aggtggttta ttgtatctga gtggcatgtg cattatggcg tacaacgtgt accgcacagc
    10621 catcggtggt aaagcagtcg atgccgaaat ccctgcggtt tcccaaacac agcaccacta
    10681 aaatactaag aaagataggc taccaaaatg aaattacaac aattggctga agaaaaaatc
    10741 ggcgttctga ttgtgttcac gctgcttgta gtcagtgtcg gtctgttgat tgaagttgtg
    10801 cccttggcct ttaccaaggc ggcaacacag ccggcgccgg gcgtgaagcc ttacaatgcc
    10861 ctgcaggttg ccggacgcga tatttacatc cgtgagggct gttacaactg ccactctcaa
    10921 atgattcgtc cgttccgtgc ggaaaccgag cgttacggtc attactctgt tgctggagag
    10981 tcggtttacg accatccgtt ccaatggggt tccaaacgta ccggtcctga tttggcacgt
    11041 gtgggcggcc gctattccga cgaatggcac cgcatccacc tgctgaatcc ccgtgatgtc
    11101 gtgcctgagt ccaatatgcc ggcattcccg tggcttgcac gcaataaagt cgatgtcgat
    11161 gcaaccgttg ccaacatgaa ggctttgcgt aaagtaggta ctccttacag tgatgaggaa
    11221 attgcgaaag cgcctgaggc tttggcaaac aaatccgagc tggatgctgt agtcgcctat
    11281 ctgcaaggat tgggtctggc tttgaaaaac gtaaggtaac atcatggata ttaacggtat
    11341 tcgcgctctt ttcacggtat ggatctttat ctgtttcctg ttagtactct atatcgtctt
    11401 caacaggcgg aataagaaaa actacgatga tgccgcaaac agcatttttg ctgaaaacca
    11461 agatgcgcaa gataagaaaa gcgaaaaccg ttaatattgt gataacggag caaaacaatg
    11521 aacgcaacat cccaatttac cagtaatttc tggaatatat atattgcagt tattgtctta
    11581 ctgagcttta tcgctttggc ttggctgctg ctgtctcaaa atgttgtcaa acgtccgaag
    11641 aagggcgaag aagtacaaac tacgggtcac gagtgggacg gcattgccga atacgacaat
    11701 ccgctgcccc gctggtggtt ttggctgtgt gttttgacgt ggctgttcgg tatcggttat
    11761 ctggttatgt atccgggtgt cggcgactac aaaggtctgc tgaaatggac cagccataac
    11821 caatatgaaa aagaggtcaa aaaagccgat gagcaatacg gcaaactgta tgccaagttt
    11881 gcggatatgc cgattgaaaa agtggcaaaa gacccacagg ccaagcaaat cgcccaaaac
    11941 ctgtttaaca cttattgtat ccagtgccac ggctctgatg ccaaaggttc taaaggtttt
    12001 ccgaatctga ccgatagcga ttggttgtgg ggcggcgatc ccgataaaat ccacgagacc
    12061 atcgaaaaag gccgtgttgc gactatgcct gcctggggtc ctgctttggg cgaagaaggc
    12121 gtgaaagacg ttgcccatta tgtgatgtcc ctttcaaaac ccaaaggtca gtatgatgag
    12181 gaacgtgccg cgcgcggaca agccttgttc agcggtccgc ctgccaactg tttcacttgc
    12241 cacggcgata agggacaagg tatccaaggt ttgggtccga acctgactga tgacgtgtgg
    12301 ttgtggggcg gtacgcaaaa atccattatc gaaaccatta ccaacggtcg cagcagccaa
    12361 atgcccgctt ggggacattt cttggataaa gacaaactgc atatcatgac tgcttatgta
    12421 tggggtcttt ccaataaaga cggtaaagct ccggtgaaaa aagccgagcc tgcaccggcg
    12481 gcagaacccg caccatctgc tcctgcagaa gcagcacaag ccgcttctga agccaaacct
    12541 gccgcagcag aacctaaagc cgaggaaaaa gccgcacctg ccgccaaagc ggatggcaaa
    12601 caggtttatg aaaccgtttg tgccgcctgc catggcaatg caattccagg tattccccat
    12661 gtaggcacca aagccgactg ggccgaccgc atcaaaaaag gtaaggacac gttgcacaaa
    12721 cacgcgatcg aaggttttaa tacgatgccc gcaaaaggag gtcgcggcga tttgagcgat
    12781 gatgaggtca aagctgcggt tgactatatg gtcaaccagt caggcggtaa gttctaaccg
    12841 ttgttttccg actttgtcgg atgcagaaat ccggattcat atcgaatccg gattttttat
    12901 tttgaacgga aatcctgttt ttgatttatg ccgtctgaaa gtggggattt tgatgcttaa
    12961 gcagtcagac cgactgatat ttttgtatgg tttaaagaat ggtgcagttg ggtgtcggac
    13021 agcattgttt gatgctttca gacggcatat aggtttgttt cttcaaaatt gaatcttttt
    13081 tcatatgttg caaaaaaatt gattttagtc ataaagaatt agtctttatt gtttataatc
    13141 gcgcatcatc acgatagcct taatttcaaa aggacgatta atggatacac aaatcaaaac
    13201 tgaagccggt cagcagaata accggcatta tctgagcgtt tggaggtggc atttttatgc
    13261 cggtttattg gttatgccct tcttgaccct gcttgccgtt acaggtttgg gtatgctgtt
    13321 gtttgccaat attaccggta aagagggaga gcgtattcac gttttccctc aggcagtggt
    13381 gcaacccctc tctgttcagg cagaagcggc acgcggtgcc attaatccgg agacttcgtc
    13441 cgtcgtccaa tatattgcac cgcgtgccga tgatatggtt gctgtgttcc gtgtcaacaa
    13501 tgagggtaag gcaaccatgg ttgcggtcga tccttatacg gcaaaagtgg tcaacaccat
    13561 gccgcgcaat cagggttggt atcacactat ggatgaaatc cacggcgata tgatgctcgg
    13621 tgcggcaggc gattatcttt tggaaacggc agcttcactg accattatta tggttgtcag
    13681 cggcttgtac ctttggtggg cgaaacagcg cggcattaaa gcgatgctgc tgccgccaaa
    13741 aggcagggcg cgttcttggt ggcggaatct gcacggcgcg tttggaactt gggtatcgtt
    13801 gattttactg ttgttctgcc tgtcgggtat tgcttgggca ggtatttggg gcggcaagtt
    13861 cgtgcaggct tggagtcagt tcccggccgg caaatggggt gtcgaaccga accccgtttc
    13921 agtcgtgccg acccacggcg aggtattgaa tgacggcaag gttaaggaag tgccgtggat
    13981 tttggagctt acgcctatgc ctgtctcagg gacgactgtg ggtgaaaacg gcattaaccc
    14041 cagcgagccg atgacattgg aaaccgtcga ccgtttcgcg cgggaaatcg gtttcaaagg
    14101 gcgttatcag ttgaatttgc ccaaaggcga ggacggggta tggactttgt cgcaggattc
    14161 tatgagttat gacatgatca gcccgtttgc cgaccgcacg gtgcatatcg accagtacag
    14221 cggcaagatt cttgccgaca tccgttttga cgattacaac ccgttcggca aatttatggc
    14281 ggcaagcatt gcgctgcata tggggacttt gggctggtgg agcgtgttgg cgaacgtcgt
    14341 gttctgcctt gccgtgattt ttatcggcat cagcggctgc gtgatgtggt ggaaacgccg
    14401 tccgtccggc gtggcgggca ttgttcctcc ggcgcaaaaa atcaaactgc ccgtctggtg
    14461 ggcgatggca ttgccgctgc tgttgattgc actgcttttc ccgaccgcgc tgcttgccat
    14521 tgccgtgatt tggctgttgg ataccttgct gctgtcgcgg attcctgtgt tgaggaaatg
    14581 gtttaaatga cccgatgccg tctgaagcgt tcagacggca ttttgtttgc gggcggcttg
    14641 cccgagaagc tatataattc cgcatcactg tcattttgaa aaagcaagcc atgttttacc
    14701 tgtaccaatc caaccgcctc gaatcgctcg ctgcactgtt tgcccgcatt caaaaagtca
    14761 aaccgctgaa atgtgccttg cagcccgaac agattgttgt gcagagccag gggatgcgcc
    14821 gctacctcaa cacctgcctc gcccgcgatt tgggcgtggc ggcgaatttg gcgttcagcc
    14881 tgcccgccgg cctgacgtgg aagttgatga aaaaactgat tcccggtatt ccggaactca
    14941 gcccgtttgc acccgaagtc atgcgctggc ggctgctgga tttgttccgc agcgcagaat
    15001 ttcaaaacgg agcggaattt gaagatgtga ggaatgtgct gcaagactat ctgggcagcg
    15061 gagaatcggc ggattaccag cttgcgggac agcttgcaga catattcgac caatacctcg
    15121 tgtaccgtcc tcagtggata gacgcttggc agcagggcag gatactcggt ttgggcgacg
    15181 acgaaatctg gcagtccaaa ctgtggcgtt acctcgacga cggcaggcag agcgcgccgc
    15241 accgtgtcgc gttgtgggaa aaactgcttg ccgcgttgga caaggataag ctgcccgagc
    15301 gttatttcgt gttcggcatt tccacgatgg cgccgatgta tttgcagctt ttgcacaagc
    15361 tgtccgaaca ttgcgacgtg ttcgtgttcg cactcaatcc gagcgggatg cattggggca
    15421 acgtcatcga agcggcgcaa atcctcaaag gcggcggcga tcccgatctg acgcaggcag
    15481 ggcatccgct gcttgcctca ttgggcaagc aggggcgcga ctttttcgac tttttgaacg
    15541 aaatggaaat agaaggcgaa acgccggtat ttgaagaagg cggacgcgat acgcttttgc
    15601 acgccctgca aaccgatatt caaaacctga aaatgccgtc tgaaaatgtg ggaagcgtca
    15661 acacgggcga cggctcgata cgcatcgtct ccgcacacag ccctttgcgc gaattgcaga
    15721 tactcaaaga caagctgttg aaaattctgc atgaacatcc cgattggcag ccgcatgata
    15781 tcgccgtatt aaccccgaac atcgaaccct atacgccttt tatcgaagcc gtgttcggac
    15841 aggcgcagcc cggcgcgcag gcattgccgt attccgtttc ggacgtgaaa atcagccgcc
    15901 ggcaaccgtt tttttatgcg ttggagtgcg tgtccgactt gctggaaagc cgttttgaag
    15961 tcggcaaagt gctcgcgctt ttggaaaccg cgcccgtgtt gcgccgtttc ggactgaccg
    16021 gggacgattt accgcttttg cacgacatgg ttgccgattt gaacgtccgc tggggtttgg
    16081 acggagaaat gcgcggcggc acggatcagc tgtttacctg gaagcaggcg gcagaccgta
    16141 tgatattggg ctggatgctg cccgaaggcg gcaacccgat gtggcaggat gtcagcgcgt
    16201 ggtatgccga cgtgaatcaa accgccatgt tcggacgttt tgccgccttc ctcgaaaccc
    16261 tgtcggatat tgcccggata tggcggcaac ctgcgacggt cggcgaatgg gtggcgcgtt
    16321 gccgggattt gcttgaaaca ttgtttcaag ccgggcccga tgaccaaaag gcagtccaaa
    16381 acctcgaaaa cgaatgggtc aaatggcagg aagagagcac tttggctggg tttttcggac
    16441 agttgccgca acaaaccgtc atccgccata tccgccgctt cctcgacagc gaaagcgagg
    16501 caggcttttt acgcggcggc atcacctttt gcagtatggt gccgatgcgg agcctgccgt
    16561 tcaaagtcat ctgcctgttg ggtttgaacg acggagattt tccccgcaat accaaagccg
    16621 ccgtattcga cctggttgcc aaacatcccg ccaaaggcga ccgcgcccgc cgcgacgacg
    16681 accgctacct gttcctcgaa gccctcatca gcgcgcgcga aatcctgtat ttgtcctaca
    16741 tcgggcgcga catccgcaaa gacgaagagc ttgccccgtc ttccctgttg ggcgaactca
    16801 tcgacacagt tgccgccatg accggcacaa acagccgcca acttgcacaa aattggatag
    16861 aacagcatcc gctgcaagcc ttctcgcgcc gatatttcca agaaggcgga cgttcagacg
    16921 gcatattcgg cacgcgtacc gactacgccg ccgcgctcgg acaaacgccc gaaccgccgc
    16981 gccctttttt cgaccaaccc gtagaaaacg ccgaacccgt tgccgaaatc ggacaggacg
    17041 aatttatccg cttctggcgc aaccccgtca aagtatggct tcagcagcag cttgcgtgga
    17101 gcgaacccca tatcggcgaa gcctgggagc ctgccgaacc cttcgagccg caacacgcgg
    17161 agcaaatcgc cgaaacctat atcggggcgc gccgcgaagg acaagatttt tcccaaaccg
    17221 ccgcccgtat cgcggcggaa agcctgctgc cttccggaga attgggaaaa ctttggcagc
    17281 agtccttcca aaccgccgcc aaacaaatcg acacggcggt tttaaacagc cccaaactgc
    17341 ccccgtttcc atacgccata ccgtcggacg ggcaaatcct gaaaggcagc ttgggcaatc
    17401 tgtaccgctg cggtcaggtg ttttatgcct acggcaaacc caatgcgccg caacgtatcg
    17461 cttttttgtt ggaacacctg atattttgcg ccgttatgcc gtctgaagcc gaaacgcggc
    17521 aaacctttat cgtcaaatcc ggagaaaccg aagtattggc ggaaatcgcg caagacaggg
    17581 cattgcagct gttgtcggaa tggattgtat ttttcaatat cgggcaaaac cgcccgctgc
    17641 cgttttttgc caaaacctcg cttgccgccg ccgaagcgtt tgcccaaaaa caagattggg
    17701 aagccgccct gaaaaaagcc caaaccgcct attacggcag caaagtcagc aaagggcaga
    17761 aagactatac cgaagtcgcc ctcgtgttcg gcaacgcaag ccaaaacccg ctcgaacagc
    17821 ccctgtttga aaacctcgcc cgcctgctcg ccgacacact tgccgcagcg gaaaagaggg
    17881 aagggaccgg agaagcctga cgggatggaa aggcaaatac cgtctgaaaa cagaaaaaag
    17941 gttttcagac ggtattttgt ttatcgtgtt taaggcaccg gatagaatgt gggtgcgccg
    18001 ggaccgacgg gcaggcccaa aacaaatacc caaatgcaga ataaggcgat ccatgcaatt
    18061 aagaagaaag cggaatacgg caacatcata gaaatcagcg tgcctacgcc cgcatctttt
    18121 ttgtatttga ttaccgtcgc cataatcagc ccgaaataac tcatcatcgg cgtaataata
    18181 ttggtaacgg aatcaccgat gcggtaagcg gcttgaatga cttcgggcgc gtaaccggcc
    18241 aacatcagca tagggacgaa aatcggtgcg gttaccgccc attgcgcgga agcggagcct
    18301 atcatcagat tgataaaagc acaaattaaa ataaaaccga taaacaacac gctgccggcc
    18361 aagccgactt cttttaagaa caccgcccct ttaacggcaa tatattgccc aatattcgtc
    18421 caattaaaaa atgcgacaaa ctgtgcggca aaaaagatga tgaccaaata aagtcccaaa
    18481 gtactcatcg attcggccat cgcattaacg acttcccgtt cgccgcgcaa acttcgggtt
    18541 atccggccat aaacaatgcc cggtagcgca aacaacaaga aaataaaaac aacaatcgat
    18601 tttaaaaacg gcgaaccggc aaccaatcct gtttcaggat gacgcaaaat accgtcggca
    18661 gggacgatgc tccaagccaa tagggcggat aaagcaataa acaccacgcc tgcccaaatt
    18721 aatcctttat attccaaagg cgtgatttca ttggaatgcc ggatgtcttt ttcttcttgt
    18781 gacaaatctg actgataagg gcccaattgc ggttcgacga ttttttcagt aacaaaataa
    18841 ccaatcaaag caatcacaaa cgtactggct gccataaaaa accagttggc ttcagggcct
    18901 acgacgtagt cgggatggat gatttgcgcc gcctgttggg taatgcctgc caagagcgga
    18961 tcgagtgtgc ctaagaacag attggccgaa taaccgccgg aaacgccggc gaaagccgca
    19021 gccagaccgg cgagaggatg gcggccgagc gaatgaaaga tgacggcgga caaagggatt
    19081 aggacgacat agcccaattc agaagccgta ttggataaaa tccctgtaaa aacaaccata
    19141 aaagtagtga gtttgcgtgg ggattttgtg agcaataagc gcattaatgc ggaaatcaag
    19201 cccgattttt ccgcaatccc cacgcccaat aaagaaacca acaccgttcc caacggcgcg
    19261 aaaccggtga aatttttaac ggtatgcgtc aggattttga tcaaaccgtc ggcatcgagc
    19321 aggctgacaa cgtgaatcaa accgtcatcg gcacgtcctt tcgccccaac aggacgcgga
    19381 tcggggacgg atagtccgaa atacgcaccg acggcagagg caatcagcaa taacacaatg
    19441 aaaataataa aaagcgtaac cgggtgcggc aacatattgc ccagccattc gactgtgcgt
    19501 aaaaatcgtc cgctacgacg cgcgtcggtt tgactcatct gcttctcctt aaaaaaatat
    19561 attgagtgca ttaaattttt gaatttaaca tgaagtgttt tgtaagcaaa ctaaaataat
    19621 cgtatatgta tataaccgta atagattaaa acggaaaatg cccacatcgt tattctcata
    19681 aaggcggtaa tccgaaaact tgacgttaaa gccttatcag gaatgactgg aactcaaaaa
    19741 aacgaattcc cgcctgcgcg ggaatgacgg gattttcggt ttttgtttgc aagaatgccg
    19801 gagaaaaatg cagtttacat tcgaatggta ttgttattaa ttgtaaataa atattggaag
    19861 gtaaatgata tgaaaaatga aattcaaaaa atcatggata aatatgatcc atggcatgaa
    19921 gatgattttg agtcgtatga agacattgcc aaagatgtat cgttgatgac agataaaacg
    19981 ttcattgaac attatttgtt agaagtttat tcagaagaaa acggacattt tgaccaagag
    20041 aatatccatg cgatgatagg ggaaattaaa aatgcaattt agtgaatatg tatttttaga
    20101 agaatgcttt gtgtcatttt gatgccgttt gaaagggttt gttcgtttca gacggcattg
    20161 ttaccaaggc ttgattattt ccggcgcagg cagggatggt tttccaagtt gtccatcatt
    20221 atcccgatga tgcgcggggc ggttttgccc aaatcgaaac tttcgccgga agagaaccaa
    20281 cgccaaatca gcccgtccaa cgtcgatttg atgaagatga ccgccgtttc cttgtccaaa
    20341 tcgtcagcca aatcctgatt ttccaccgct tcggtcaaaa cggcggtaat tttctcgcgc
    20401 cagattgcct gatgcttgcg ggcaatggcg ataacggcgg cgttttgctc cgtgtgttcg
    20461 cattttaaaa acaggatgtt gtggaatttg tagtagatgt cgttgctttg cagccgctcg
    20521 aaaaagtgca gcagcgtgtg gcggaatacc gtccaagaac ctccttcggc atctgcggca
    20581 tcttgcgcga tgcagttttc gatgtcgtcg cagatacgtt ggaacaacgc gtcaaacaag
    20641 tcttccttat ttttgaaatg ccaatagagc gcgccgcgcg ttacgccggc ggcttgggcg
    20701 atttcgttga gcgaggtgcg ggcaatccct ttgcggtaaa aggtttccaa ggcggcaagc
    20761 atcaggtgtt ctttggtttt caaggcttcg gttttggttt ttctcataat ggcgttttcg
    20821 tttcgggtcg gtttgacgag ggcggattat aaaaaagact tttatccgtg caatcgtgta
    20881 tgtataatga aacccataaa attgagactg catctcaact ttgaaaacag gtataacctg
    20941 ctttgcaacc cgttcgaaca ttcgggtatc aaagcaggcg gtttttatat cctgaaataa
    21001 gaatgcctgt tgattggcaa aaccctttac atcaaaccag acagatagga taaataatgg
    21061 ctttttatgc ttctaaggcg atgcgtgcgg ccgcgttggc tgccgccgtt gcattggcac
    21121 tgtcgtcttg cggtaaaggc agagacgcgg cgcagggcgg gcagcctgcg ggtcgggaag
    21181 cccccgcgcc cgtcgtcggc gtcgtaaccg tccatccgca aaccgtcgca ttgaccgtcg
    21241 agttgccggg gcgtttggaa tcgctgcgta ccgccgatgt ccgcgcccaa gtcggcggca
    21301 tcatccaaaa acgcctgttc caagaaggca gttatgtccg cgccgggcag ccgctgtatc
    21361 agatcgacag ttccacttat gaagcaggtc tggaaagcgc gcgcgcgcaa ctggcaacgg
    21421 cacaggcaac gcttgccaaa gcggatgcgg atttggcgcg ttacaaaccg ctggtttccg
    21481 ccgatgccat cagtaaacaa gagtacgatg ctgcggtaac ggcgaaacgt tctgccgagg
    21541 cgggcgttaa agcggcgcag gcggcgatca aatccgccgg catcaatctg aaccgttcgc
    21601 gcattaccgc gccgatttcg ggctttatcg gtcagtccaa agtttccgaa ggtacgctgt
    21661 tgaatgcggg cgatacaact gttttagcca ccatccgcca aaccaatccg atgtatgtga
    21721 acgttaccca gtctgcatcc gaagtgatga aactgcgccg gcagatagcc gaaggcaagc
    21781 tgctggcggc ggatggtgcg attgcggtcg gcatcaaatt tgacgacggt acggtttatc
    21841 ctgaaaaagg ccgcctgctg tttgctgatc cgaccgttga cgaatcgacc ggtcagatta
    21901 ccttgcgcgc cgccgtatcg aacgatcaga atatcctgat gcccggcctg tatgtgcgtg
    21961 tgctgatgga tcaggtggcg gcggataatg cattcatcgt tccacagcag gcggtaacgc
    22021 gcggtgcgaa agataccgtg atgattgtga atgcccaggg cggtatggaa ccccgcgagg
    22081 taacggtcgc gcaacagcag ggtacgaatt ggattgttac gtcgggtctg aaggacgggg
    22141 acaaggtggt tgtggaaggc atcagtatcg ccggtatgac gggggcgaaa aaggtaacgc
    22201 ctaaagaatg ggcgccgtct gaaaatcaag ctgccgcccc tcaagccggc gttcagacgg
    22261 catctgaagc caaacctgct tctgaagcga aataaggaag gcatcgatgg ctaaattctt
    22321 tatcgaccgc cccattttcg cgtgggttat ttcgattttc attattgcgg caggtatttt
    22381 cggcattaaa agcctgccgg tttcgcaata tccgtccgtt gccgccccga ccattacttt
    22441 gcacgccatt tatccgggtg cgtccgcaca agtgatggaa ggcagcgtac tttccgtgat
    22501 cgaacggaat atgaacggcg tggaaggttt ggactatatg tccacttccg ccgattcgag
    22561 cggcagcggc agcgtgagcc tgacctttac gcccgatacc gacgagaatc tggcgcaggt
    22621 ggaagtgcag aacaagcttt ccgaagtatt gagcacgctg ccggcaaccg tccagcaata
    22681 cggcgtaacc gtatccaagg cgcgttccaa tttcctgatg attgtgatgc tttcgtcgga
    22741 tgtgcagtca accgaagaga tgaacgacta cgcgcagcgc aacgtcgttc ccgaattgca
    22801 gcgtatcgaa ggcgtggggc aggtccgcct gttcggcgcg caacgcgcga tgcggatttg
    22861 ggtcgatcct aagaaactgc aaaactacaa tttgtcgttt gccgatgtcg gcagcgcgtt
    22921 gtcggcgcag aatatccaaa tttccgcagg ttccatcggt tcgcttcccg ctgtgcgcgg
    22981 acagacggtt acggctaccg taacggcgca agggcagttg ggtacggcag aagaattcgg
    23041 caacgtcatc ctccgcgcca ataccgacgg ttctaatatt tacctgaagg atgtggcgaa
    23101 agtcggattg ggtatggaag actattcttc ctcaacccgc ctgaacgggg taaataccac
    23161 cggtatggcg gtaatgttgt ccaacagcgg caatgcgatg gcgacggcaa aggcggtaaa
    23221 agaacgcttg gcggtgctgg aaaaatactt tccgcagggt atgagttgga aaacccctta
    23281 cgatacttcc aaattcgttg aaatttcgat tgaaaaagtg attcacactt taatcgaagc
    23341 gatggtgctg gtgtttgtcg taatgtacct cttcctgcaa aacatccgtt atacgctgat
    23401 tccgaccatc gtcgtaccga tttccctgtt gggcggtttc gccttcatct cttatatggg
    23461 gatgtcgatt aacgtattga cgatgtttgc gatgacattg gtcatcggca tcgtggtcga
    23521 tgacgcgatt gtggtggttg aaaacgtcga gcgcattatg gcgggtgaag gcctgccgcc
    23581 caaagaagcg accaaaaaag cgatgggtca gatttcgggc gcggtcatcg gtattaccgc
    23641 cgttctgatt tccgtgttcg tgcctctggc gatgttcagc ggggcggccg gcaatattta
    23701 caaacagttt gccctgacga tggcgtcatc aatcgcattc tccgccttcc ttgcgctgac
    23761 ccttacccct gctttgtgtg ccacaatgtt gaagacaatc ccgaaagggc atcacgaaga
    23821 gaaaaaaggt ttcttcggct ggttcaacaa gaaattcgac agttggacgc acggctacga
    23881 aggccgggtt gccaaagtgc tgcgtaagac tttccgcatg atggttgtct atatcggctt
    23941 ggcggttgtg ggcgtgttcc tgtttatgcg cctgccgact tcgttcctgc cgaccgaaga
    24001 ccaaggcttc gttatggtca gcgtgcaact gcctgcgggt gcgaccaaag agcgcaccga
    24061 tgcgacgcta gcacaagtta cccaactggc gaaaagcatt cccgaaattg aaaacatcat
    24121 taccgtttcc ggcttcagct tttcgggcag cggtcagaat atggcgatgg gttttgccat
    24181 attgaaagat tggaacgaac gtaccgcctc cggcagcgat gccgttgcgg ttgccggcaa
    24241 gctgacgggt atgatgatgg ggacgcttaa agacggtttc ggcatcgccg tcgtcccgcc
    24301 tccgattctg gagttgggca acggttcggg cctgagcatc aacctgcaag accgcaacaa
    24361 taccggccat accgcattgc tggcgaagcg caacgagctg attcagaaaa tgcgtgccag
    24421 cggtttgttt gaccccagca ccgtccgtgc cggcggtttg gaagactcgc cgcagttgaa
    24481 aatcgacatc aaccgtgccg cagcggcggc gcaaggcatt tcgtttgccg acatccgcac
    24541 cgcattggca agcgcgctga gttcgtctta tgtcagcgac ttcccgaacc aaggccgtct
    24601 gcaacgcgtg atggtgcagg ccgacgagga tgcccgtatg cagcctgccg atattttgaa
    24661 cctgaccgtg ccgaacaaat ccggcgtcgc cgtaccgctt tccaccattg ctactgtttc
    24721 ttgggaaaac ggtacggaac agagcgtacg cttcaacggt tatccctcga tgaagctgtc
    24781 cgcttcgcct gcaaccggcg tttccaccgg gcaggctatg gcggcggttc aaaaaatggt
    24841 tgacgaattg ggcggcggtt acagcttcga atggggcgga cagtcgcgcg aagaggcaaa
    24901 aggcggctcg caaaccctga ttttgtacgg tttggcggtt gcagccgtat ttttggtact
    24961 tgccgcgctt tatgaaagct ggtcgattcc gctggcggtc atccttgtga ttccgttggg
    25021 tttgatcggt gcggctgcgg gcgtaaccgg gcgcaacctg tttgaaggac tgttgggcag
    25081 cgttccctcg tttgccaacg acatctactt ccaagtcggt ttcgttactg tgatgggttt
    25141 gagtgcgaaa aacgcgattc tgattatcga atttgccaaa gaccttcaag cgcaagggaa
    25201 aagcgcggtc gaagccgctt tggaggcagc ccgcctgcgt ttccgtccga ttatcatgac
    25261 ctcgtttgcc tttattttgg gcgtggttcc cctgtatatt gccgctggtg cgagttctgc
    25321 cagccagcgc gccatcggca cgaccgtatt ctgggggatg ctcatcggca cgctcttgtc
    25381 cgtgttcctt gttccgcttt tttatgtagt ggtgcgcaaa ttcttcaaag aaactgcaca
    25441 cgagcatgaa atggcggtca gacatgcatc aaaagcgggt ataaccggtt cggacgataa
    25501 acaatattaa gcaaaatacc gtctgagaac cgccggtttt cagacggcat caggactttt
    25561 ttatgaatac tacattgaaa actaccttga cctctgttgc agcagccttt gcattgtctg
    25621 cctgcaccat gattcctcaa tacgagcagc ccaaagtcga agttgcggaa accttccaaa
    25681 acgacacatc ggtttcttcc atccgcgcgg ttgatttggg ttggcatgac tattttgccg
    25741 acccgcgcct gcaaaagctg atcgacatcg cactcgagcg caataccagt ttgcgtacag
    25801 ccgtattgaa cagcgaaatc taccgcaaac aatacatgat cgagcgcaac aacctcctgc
    25861 ccacgcttgc cgccaatgcg aacggctcgc gccaaggcag cttgagcggc ggcaatgtca
    25921 gcagcagcta caatgtcgga ctgggtgcgg catcttacga actcgatctg ttcgggcgcg
    25981 tgcgcagcag cagcgaagca gcactgcaag gctattttgc cagcgttgcc aaccgcgatg
    26041 cggcacattt gagtctgatt gccaccgttg ccaaagccta tttcaacgag cgttatgccg
    26101 aagaagcgat gtctttggcg cagcgtgtct tgaaaacgcg cgaggaaacc tacaagctgt
    26161 ccgaattgcg gtacaaggca ggcgtgattt ccgccgtcgc cctgcgccag caggaagcct
    26221 tgattgaatc tgccaaagcc gattatgccc atgccgcgcg cagccgcgaa caggcgcgca
    26281 atgccttggc aaccttgatt aaccgtccga tacccgaaga cctgcccgcc ggtttgccgt
    26341 tggacaagca gttttttgtt gaaaaactgc ctgccggttt gagttccgaa gtattgctcg
    26401 accgtcccga catccgcgcc gccgaacacg cgctcaaaca ggcaaacgcc aatatcggtg
    26461 cggcgcgcgc cgcctttttc ccgtccatcc gcctgaccgg aagcgtcggt acgggttctg
    26521 tcgaattggg cgggctgttc aaaagcggca cgggcgtttg ggcgttcgcg ccgtctatta
    26581 ccctgccgat ttttacttgg ggaacgaaca aggcgaacct tgatgtggca aaactgcgcc
    26641 aacaggcaca aattgttgcc tatgaatccg ccgtccaatc cgcctttcaa gacgtggcaa
    26701 acgcattggc ggcgcgcgag cagctggata aagcctatga cgctttaagc aaacaaagcc
    26761 gcgcctctaa agaagcgttg cgcttggtcg gactgcgtta caaacacggc gtatccggcg
    26821 cgctcgattt gctcgatgcg gaacgcatca gctattcggc ggaaggtgcg gctttgtcgg
    26881 cacaactgac ccgcgccgaa aaccttgccg atttgtacaa ggcgctcggc ggcggattga
    26941 aacgggatac ccaaaccggc aaataaccgc ccgggcaaac aaatgccgtc tgaaacgata
    27001 aggtttcaga cggcatttct ttctttatag tggattaaca aaaaccagta cggcgttgcc
    27061 ccgccttagc tcaaagagaa cgattcccta aggcgcccaa gcaccgggcg aaccggttcc
    27121 gtactatttg tactgtctgc ggcccgccgc cttgtcctga tttttgttaa tccactatat
    27181 gtttgacggt tgcggtcagg ctgcgcggcg tgccgtaaac gtgaatgtcg ggcatcgtgc
    27241 ggcagcgttt gttaaagacg ttttcaaagt ccaaaccgat gcgggtggat ttgccgattt
    27301 tgtaatgcgc ggtcaagtcc agtgtagcgt atggggcgtt gggtcaatgc ggctgcggcg
    27361 acagggaaga aaccgccata cccgtcggca tcccgaagcc gaatacggca aaggcaagcg
    27421 caatcagcag cgtataaagc cccgcctgat agcctttcag cttcaggacg gtcagcgcgg
    27481 caaagaaaaa gacgatgggt aagagtgcgg cggcggcagt cagatacagg ctgccgccga
    27541 ttgccgtgta gttttgaacc caagtttcca taattgaaca tctccgaaaa tatttttcta
    27601 atcgtcggca atagtggtca aaccaattaa agcaacgttg cattacttta cgaaacttta
    27661 atattcaggt caatatattt ttgggcggtt cggcagattt gaatcggagc tttcatttaa
    27721 atcccgttaa aacaaatatt tgcatgaaca aaaattgtag tttggtgtag ttttttcctg
    27781 tgtttcgggg gcggtgcgag gtaagtgccg ttgacggcgg gaacggcgtg tcattagaat
    27841 ccgccctgat tggccagtcc aatttgatgt ttgatgtatt tcggaagggt tctgcaggga
    27901 atctttataa aggaggagtg atgaaactgg taaggcctca gaaaatcagc gatcaggtat
    27961 tgtcggtatt ggaaggccgc atcgtggaag gggtttacgc ggaagggggc aagattccgc
    28021 ccgaacgcgt tttggcggaa gagttcggcg tttcgcgtcc gtcggtccgg tcggcattga
    28081 atattttggt tgcccgtcag attttggaag cgaggcaggg cgatggttat tatgtttcgg
    28141 tcaagccgca gcaggatttt ttgcaaagtt ggcaggagct tttgggcaaa cattccaatt
    28201 gggaacagga tgtttttgat ttcagctgcc acatcgaggg ctgtatggcg gcattggcgg
    28261 cagaacgccg gacggatgcc gatttgaagc ggattcggtt ttggcttgaa aagtttgagg
    28321 aagcgtgcgg aagcggcaat ctggaacatc agggcgaagc cgatgtcagc ttccatcaga
    28381 cgattgcgga tgcggcgcac aatttgttgt tcagccattt gtcgggcggt ttgttgaaaa
    28441 tgctgtaccg gcagacgcgc agcagcctta tttatctgaa tcaggaagaa gatccgcgtc
    28501 cgaaactgat ggcgcagcac cgcgtgctgt atgaggcgat atcgaaccgc cggccgggcg
    28561 aggcttcgga ggcggcaaag gctcatttga attatgtggc gagcagcata ttgaaagaca
    28621 gggaatatca gagccgtaac cgccacgcgg atactttggc gcaaaacgat ttgaagcgcg
    28681 tgcaggattg ggaggtatga ataccgttca gacggtctgg catattcgga tgtatcggta
    28741 tagtgaacta aatttaaacc agtacggcgt tgcctcgcct tgccgtacta tttgtactgt
    28801 ctgcggctcg ccgccttgtc ctgatttaaa tttaattcac tatagtatta cccgattcga
    28861 acgggcggat aatgaaatgc cgtctgaaca gcatttgcgg ttcggacggc attttgtttg
    28921 cggggcggag gcgtttgctt agaagccttg cgccagcatc gcatcggcaa ctttgacgaa
    28981 gccggcgatg ttcgcaccgt tgacgtagtt cactttgtcg ccgactttgc cgtatttcag
    29041 gcaggactcg tggatgcttt gcatgatgcc gaacagtcgg ctgtccactt cttcgcgcgc
    29101 ccaagacagg cggatggcgt tttggctcat ttccaagccc gaagttgcca cgccgccggc
    29161 gttggaggct ttgcccggag cgtagaggat gccggctttg ataaattgct cgaccgcgcc
    29221 caaagtcgac ggcatattcg cgccttcggc aacgacgtgg cagccgtttg ccaacagggt
    29281 tttggcggct tcttcgtcca attcgttctg ggtcgcgcag ggcagggcga tttcggcggc
    29341 aacgccccac ggtttttggt tttcaaagta ttgcaaacct tgctctttgg cgtaggtggc
    29401 aacgcgttcg cggcggactt ctttcaattc gatcaaggcg gcgagttgcg cttcggtcat
    29461 accgatgtcg gggaagagga cgaagccgtc ggagtcggag acggtcagca ctttcgcacc
    29521 cagttggatg gctttttcag cggcgtattg cgccacgttg ccggaaccgg aaatcagaac
    29581 gcgtttgcct tcaaaactat cgttgcgggt ttgcagcatc gcttgggcga aatagacgca
    29641 gccgtagccg gtcgcctcgg ggcggatgag gctgccaccc cattccaaac ctttgccggt
    29701 caggacggaa gtgaattcat tgcggatttt tttgtattgt ccgaacaggt agccgatttc
    29761 gcgtccgcct acgccgatgt cgccggccgg aacatcggtg tccgcgccga tgtggcggta
    29821 gagttcggtc ataaaggctt ggcagaagcg cattacttcg gcatcggatt tgcctttggg
    29881 gtcgaagtcg gaaccgcctt tgccgccgcc cataggcagg gtggtcaagg cgtttttgaa
    29941 cacttgttca aaagcgagga atttcaacac gcccaaatcg acggtcgggt ggaagcgcag
    30001 gccgcctttg taagggccga tggcggaact catttgcacg cggtagccgc ggttgacttg
    30061 gacttgtcct ttgtcgtcct gccaggttac gcggaacatc acgacgcgtt cgggttcgac
    30121 gatgcgttcc agcaggcttt gctgggtgta tttcggattt tttgccaaaa acggatcaag
    30181 gctcatgaag acttcttcaa ccgcctgatg gaacggctcc tgattggggt tgcgttgttt
    30241 gaggttggca aacagggtgt tcaggtcagt cattcggata ctccttttgg ttgatattgt
    30301 actaatggga aaacaaattt gtagttggca atataaacct atttttttct gatgccaagc
    30361 ctaattttgc acaaattgtt gacattattt ttcccatatt ttcgatatat gatgtttatt
    30421 gccgataatc aaccaataat cataaattaa tttctcaaat aaaaaatatt attggaattt
    30481 tattctaagt agttaaacta gattttttga ttccaatcaa gataattttg ttttcacgcc
    30541 tgcttttgct gtgaaaaagg gctgaaattg ccaacaatcc tgcctgcctt catccgcgtt
    30601 ttgagcgtcc ggcggggcgg cctgcgcgtt tgtccggact cgggtgttgg ccggaaatgt
    30661 tacaatatgt gccgtttaat ttgatgaaag atgatgatga aggcctattt ggacttgatg
    30721 cgccacgtcc tcgacaacgg tacggacaaa tcagaccgca ccggtacggg tacgcgctcc
    30781 gtattcggtt atcagatgcg ttttgacttg ggcaaaggtt ttccgctgct gacgaccaaa
    30841 aagctgcatt tgcgctcgat aatccacgag ctgctttggt tcctcaaagg cgataccaat
    30901 atcaaatacc tgaaggataa caatgtttcc atttgggacg agtgggcgga cgaaaacggc
    30961 gacttgggcc cggtttacgg ctaccaatgg cgcagctggc ccgcgcccga cggcaggcat
    31021 atcgaccaaa tcgccaatgt ggtggaacaa attaagaaaa accccgactc gcgccgcctg
    31081 attgtgtcgg cgtggaatcc ggctttggtc gatgagatgg ccttgccgcc ctgccacgcg
    31141 ctgtttcagt tttacgttgc cgacggcaaa ctgtcctgcc agctttacca gcgtagtgca
    31201 gatattttcc ttggtgtgcc gtttaatatt gccagctacg cgctgttgac gatgatgatg
    31261 gcgcaagtgt gcgggctgga ggcgggcgag tttgtccata cgtttggcga cgcgcatttg
    31321 taccgtaacc atttcgaaca ggccgcattg cagttggaac gcgaaccgcg cgccttgcct
    31381 gtgatgaaaa tcaatcctga agtcaaggat ttgtttgcct tcaagtttga agatttcgag
    31441 ttggaaggct acgatccgca tccgcacatc aaggcggtag tgtcggttta ggaggcggta
    31501 tgggcaggca tttcgggcgc agacgttttc tgacggctgc cgccgttgct gtggccggtg
    31561 cggcggtttc ttttttgccg aatccttttg ccgccggcgg cgaaaaacgc aacatggata
    31621 aaaaacgcga tgaaaatgtg tttttctgga aaggtgtcgc gctgggttcc ggcgcggagc
    31681 tgcgcctgtt cggcgtggac gacagacagg cggcggattt ggtcaataag gttttggcgg
    31741 aagtggcgcg tttggaaaaa atgttcagcc tttaccgtga agacagcctg atcagccgtc
    31801 tgaaccgcga cggttatctg acttcgcctc cggcggattc tttggaactg ttgagcctgt
    31861 gccgcgatat tcacgcgctg acggaaggcg cgttcgatcc gacggttcag gcgttgtgga
    31921 aactgtatgc cgattatttt gccgcccgtc ccgatgcgga aacgccgccg ccggagaata
    31981 gcgtgaagga aaccctgaaa cgggtcggct tcggcaaagt ctcgtttgac gaccgtgaaa
    32041 tccgttttgc cgaaaagggt atgggtttgt ccttgaacgg catcgcgcaa ggctatatca
    32101 cggacaaggt tgttgcgctg cttaaagcaa acggagtgcc tgccgcgctg gtcgatatgg
    32161 gcgaaatcag ggggtttgac aaacggcagg cggatgtgga atgtcggcat ccgcaatccg
    32221 gacgacgaag aaggcgtgtt ggcaaatatc acggtgaaag acaaggcgtt tgccacttcg
    32281 ggcggctatg ggacggtgat ggacaaagcg ggcaggctta cccatttgtt cgatccgcga
    32341 accggcgtga gtacgccgcg ttataaaagc atgagcgtaa tggcggatga tgcggcggtt
    32401 gccgatgcct tgtcgacggc gttttccgtg atggatttgc cgctgatacg gtcggttgcc
    32461 gaaagcaggc ggcttaaagt ttggctggcc atgccggaca atatagtgga ttaacaaaaa
    32521 ccggtacggc gttgcctcgc cttgccgtac tatctgtact gtctgcgact tcgtcgcctt
    32581 gtcctgattt aaatttaatc cactatactt tgaacgaaat aggttgaatc tgaagcaaaa
    32641 tgccgtctga aaggctttca gacggcattt tgccgtcggg cggtatgttt aaaggatgcc
    32701 taaagaattg acgaaaacaa taataatggc aatcggggca aggtagcgca aggtattcag
    32761 ccacagcagc agcactgccc gtggtacggt gctgcccgcg ccggcatctt ttaacacaga
    32821 ctgcttgtcc tgaatccagg cggtaaagat ggaaacactc aaagcaccaa tcggcataat
    32881 gacggcggaa ataacatagt cccacaaatc aaaaatggtt ttgccgaaaa ccttaaactc
    32941 accccatgcg ccgaaagaca gcgcggacgg gatgccgaca atgaaaatag ccgtgccgat
    33001 aagccaagta tgttttttgc gtttgcgctc gtcttggcgg atggttgagg caatgaccgt
    33061 ttccaacatc gaaaatgccg aagtcagcgt ggcgaaaacg accagaagca tgaatactgc
    33121 aaataaaacc gtaccgaacg gcattttcat gaacactgcg ggcaatacga taaaaatcaa
    33181 tcccggcccc tggttcggtt caaaaccgaa ggcgaatacc gccgggaaaa tcaccaggcc
    33241 ggcaagcagg gaaaccaaga ggttcatcca cataatcgta tggccggaac ggaacatatc
    33301 ctgatctttt cccaaatacg aagcataggt aatcatggcg gaaacgccga tgctcagggc
    33361 aaaaaacgcc tggcccaaag ccgtaatcat cgtatcggct ttaaagtacg accaattggg
    33421 tttgagcagg aaagacacgc cctccattgc atccggcagc gtcagcgaac ggacagccag
    33481 cgcaataaag aggataaaca gccccggcat cagataacgg tttgcctttt caatgccgtc
    33541 tgaaatgccg cctttgacca cccaaaccgt aatcagcata aacagtgcct gataggacag
    33601 cgaacctgcc ggattggaaa tcgttgtgcc gaacagggct tcaaagtccg ctcccgcatg
    33661 gattgcccct gtaaaactgt ggacgacata attcaatacc catccgccga ccacgctgta
    33721 aaacgacagc aaaataaagc aggcggcaac gcccatgcgc ccgacccaaa gccattgcgt
    33781 acccggacgc agaaccctga aggaatcgac ggcattttta ccgcccgtgc gcccgatata
    33841 aaattcggca agctggacgg gcagggcgac caagacggta aatatcaaaa acagcaggaa
    33901 aaacaccgcg ccgccgttgg tgcccgccgt ataaggaaat ttccaaatcg cgcccaaacc
    33961 gatggccgaa cctgccgcag caagtacgaa accgattttg gacgaccaag aagtatggtt
    34021 actcataaaa tttattaaga aatgtgaagg tagtgcattg taacaaagca cttgacgcgg
    34081 cggcacaagc cggggaagac gctgcaaaac tttgccaata ggctgcgcga tatcggggga
    34141 tattgggatt ttcttatttt gaaggatgaa ttcggggtgt ttttttccaa tgaacgaata
    34201 taatttggcg gtttgttgca tcaaaggctt tcagacggca taggccgtct gaaagcggct
    34261 tatattgcat ccaataattt gtcggcggta tggttgatgg tgaaacgggc ggcgacgcat
    34321 tgatgtcctg ccgttgccag tgcgtttttt gcttcggggt ttaagacgag gcgttcgatt
    34381 tcgtccgcca aagattcagg cgtaagcctg tccagcagaa tccccgattg atgatgttcg
    34441 ataatttcct tttgcgcacc caaagtattg gaaatcaccg ccgttcggca gtacatcgcc
    34501 tcgcataatg acaaaccgaa tgcctccggg acgaggctgg gcaaaaccac gacatcgctt
    34561 tggcggtaaa acgaagcaag tttttcggta aacccttcaa aagaaacaaa tggttctgct
    34621 cccgaagcag atacgccccg cttcaggcgg cacatataat ccggatgtcc gtgccctgcc
    34681 aatttgagcc tgatttgagg atatttccga tgcagtatca cacaggcttc aatcaggttt
    34741 tccaatcctt tttccggact gatcctgccg gcgtaggcga cggtaaaaaa acggctgtcg
    34801 ggtttttctt gagacggggg gaaccggccg gtatcgatac cgttatgaac aatccggtat
    34861 ttttctttaa agggattgtc ggcggtttgc acatcgtaaa ccagacggga aacgcaaata
    34921 aagcggtcgg tgtttttctg tatcaggcgg tgataaaaat cggttttgtt ggcgacgaca
    34981 ttatgtttga caaatatcag gcgcaccccg gtcagttttt tcaaaagtac ggataaggcg
    35041 gcaattttgc cggtgtgtat catcaggtgg gaaatgtggt tttcccggat aaagcgggta
    35101 agcgcgtaga gcgaaaacag cccgttgaac gtgtgaaggc gcgttgtgaa aacggaagaa
    35161 acttcggaaa agcgtctgcg catcaattca tcatttttat tgacggcggt aaacatggtg
    35221 cagccccgaa gtcccaatgc ttttgaaaca tcatagacat attgttcgcc cccaccccaa
    35281 atcttggccg atacgatatt taatatcctg aattcttttt ccatttaaaa ttttccgccg
    35341 gatttgtaca gataatatag tttgacatat ttaatcatcg tgtaatagct gtggttgacg
    35401 gacataatcc aacccatttt tccatcaaga aaccctttgt tcaggatata aattttgaaa
    35461 aaaccccaaa tcggacggag gataatgtcc ctaacgaaac tcacgggctt tccctgctcc
    35521 cggtatttct cggccgagat ggaagtatat ttgttgaact tgttgaaata ttgttcccag
    35581 ttgtcgtacg tgtaatggta cataaaatgt ttcagacggc gttcggggta gggggtctgt
    35641 acggtttcgt gtactttgcc ttgcaccgaa ccgccttttt tcggcatcag acggcatacg
    35701 ctgtcgggac gcatcgcgcc gtgtgtggcg ggatggttgg ggaaaaggtt gcggcgttcg
    35761 acaaaatagg cggcataatc gccggttcgg acaatttttg agatttcatc agatagttcc
    35821 ggcgtgcagc gttcgtctgc atcaatcagg aaaacccatt ctccgcctgc ctgttcgatg
    35881 gcaaatgttt tttgcgctcc gaaatccccg ttcaaatgcc gtctgaagac ttttgcaccc
    35941 aaaccctcgg caatttcggc agtgttgtcg gcgctatggt cgtcgataac gataacttct
    36001 ttatcgaaac ggcaactttc aatacattcc cgaatgtggt ttgcttcgtt tttggcaaca
    36061 atcaatacgc tgactttttt cattatcaag gtttctcaaa gcattttggt ttcacagggc
    36121 ggcaaaaaag cccaaacata cgtccgggct tgggaattgg tgggtccggt ggggttcgaa
    36181 cccacgacca agggattatg agtcccctgc tctaacccct gagctacaga cccgtcaaga
    36241 gtaggcgcgt attgtatagc atagcggtgg gattgacaaa cttatttcat ccgatgatta
    36301 agacgcagtt gcttcccccg aaggcaaacg acgagctggc ggcaatgcgt ttttcggttt
    36361 cccagccgtc gccggtcagg ttaatggcgg gcaggtcggg gtcgttctgc ccgtcccaaa
    36421 gccggggcgg aagtttccct tcgggattgc tttgccggtc ggcaatgccc cacgcgaacg
    36481 cggcttcgat tgcgcccgcc gcgcccagcg tgtgtccggt ttgcggcttg gcggacgtgc
    36541 agggcgtatt gttgccgaaa accgctgcaa cggcgcggct ttccatactg tcgttgtggt
    36601 gcgtcccggt gccgtgcaga ttaatccggc cgatgtcttc gggcgcaagg ccggcgtgct
    36661 gcaatgccgt ctgaaaggcg aggattgcgc ctttgcgtcg gggcggggcg tggaaatatg
    36721 gtaggcatcg ctgcttgcgc cgtaacccag aagctgcata ccgccggaaa aatccgcatc
    36781 gcgcgtcatg atgaatacgg ctgcgccttc gccgatattg atgccgtcgc tgttggcgcc
    36841 gtagccgacg agttcggcgt aaattttcgc accgcgccgt ttggcgtgtt ccaattcttc
    36901 caatacgaaa atccccgcgc cttttctccg atgaccagcc cgtcgcggtt cgcgtcgtat
    36961 ggacgcgggg tctcttccgg ttcgccgttg cggcggctgg cggcatagag cgagtcgaaa
    37021 acatacactt cggacgggca aaattcttcg ccgccgcccg ccagcattat atcggtcaga
    37081 ccgtatttga tggcctcgta agcataacct atgccttggc tgcccgaaga acacgcactc
    37141 gatgtcggga tgatgcgtcc ttattaatcc gaaaaagatg ccgatattgg cgtcggcggt
    37201 gtgcggcatc atacgcacat aggtattggc gccgaagttg cgcgacgtgc cggtcagcaa
    37261 caattcgccc acatcgccga tgtctttggt gccgccgccg gaagagccgc aggcaacgcc
    37321 catccgtccg tcggtaatgc tttcgtcccc gagcaaacct gcatccatca aagcctgctc
    37381 cgccgcatcg acgcacaggt acgacacgcg ccccatactt ctgagccgct tgcgcgtcca
    37441 atgtttcggc ggcgcgtaat cctcaatcgg cgcacccagt tgcgcttcca attcggggaa
    37501 acgttcgcgc caatccatat atttgacggc gtttttttcg gctttgaatg ctgcctgtat
    37561 gctttgccaa tcccggccga aggcggtaat gccgcctatg cccgttactg cgacccttct
    37621 ggtattcaac acaaacctcc gttcaccgca atcacctggc gcgtgatata cgccgctttt
    37681 tcatccatca ggaagtgcac tgcgtgcgcc acttcttccg gcagtcccat acgcgcggcg
    37741 gggacggctt tcaaaatttc tccgacgggt acgttttcat cgacaatctc cgtatcgatc
    37801 aaacccggcg ccacgcagtt gacggtgatt ttgcgtttcg ccagttcgac cgccaaggct
    37861 tttgccgcgc cgataatgcc cgcttttgac gcgctgtaat tgacctgtcc ccggttgccc
    37921 gtcaggccgg acaccgatgc catacacaca atccgtccgg ctttgcggcg gcgtatcatc
    37981 ggcataacca gcggatgcaa tacattgtaa aaaccgtcca aattagtccg cggcaccaca
    38041 tcccaatcgt cgtctgaaaa cgcggggaag gcattgtcgc gcgtcaggcc ggcgttcaac
    38101 accacgccgt aatacgcgcc gtttgcttcg atgtcggcgg tcagaatctc gcggcaggct
    38161 tcgcggtcgg acacgtcaaa ctgcaacacg cgcgcatttc tgcccaaagc gcggatttct
    38221 tccgccacgg cttcggcttc gtcgcggcgg ctgcggcagt ggacggcgat atcaaagccg
    38281 tcgtccgcca aaaccgagtg cggcggcttt gcctatgcct ctgtttgagc cggtaatcag
    38341 gacggtttca gtcatggttg ttctccgtat gtgcgtgcct gttcagacgg cattaagttg
    38401 tttcgacagg gtgttcgggg ctgtacacgt tgagtgaggc gcgcgccaaa atgccgtctg
    38461 aaaggagtgt ttcagacaag gtttccggcg cgtccgtcca acgcagttcg cagtcggaca
    38521 cgcccatacc ccggcatctt ggatagacat atgcgccgtt gccggcaaat gtgttccgac
    38581 agggacggat tgggtaaaaa tttcaagttt gcgcgtgccg ggcaggaaaa ccgagccgga
    38641 ccggttgcgc cttttttcgg gcttggaggc cggcatatgc gccgacagcc tacgccatca
    38701 gttcgataaa tgccgtatag ggcagcatgc cgccggccag caggatgtgg tcgggtttta
    38761 cctgcgcccc tgcttcgaca aaatcatcgc cgtatcgggt aatgcggtct atcagaacca
    38821 tacgcccgct gtgcggcagc agggaggcgg tgtcggtaat cgggcattat aatgataaat
    38881 gtaaattcag gctaattcgg gcggtttgcc gaggggcggc gttgtcggaa aaaatggcgt
    38941 ggatgcgttt gaatttcggc attgtcaggg ggatgggctt tggttagaat gcggcacagt
    39001 gtaaattttt gaaagaaact atgccgccga ttatttgccg gttccacttc gacatcgccg
    39061 actggcgggt ttcgggcggc aaaatgcgcg atatggccca gtagaggcag tgggcggaat
    39121 gtccggattt tgccgacggt ttgcccgatg tcaggcggga attgccgttc ctgcccgcta
    39181 tgcggcgccg ccgtttgagt aaggcggcgc gtttggtatg cgacgcggct tgggacattg
    39241 cctccgccca tcccggcagt ccggtagttt atgcctcgca cgacggggaa atggcgcgta
    39301 gtttcgattt gtggttggaa ctgttgaaat cgcataccgt gtcgccgact tcgttcggtt
    39361 tgtcggtgca caatgcgacg gcggggcaat ggtcgatatt gaggcgggat atgagcgagc
    39421 agacggcatt ggccgtgtgt gccgacggtg tggaaacggc attggcggaa gcggcgtccc
    39481 tgttggaaga gggatgcggg tcggttttgg ttttggcggc ggacgatccg ctgccggaag
    39541 gatatgccgt atcggcaacg cgcgcgccgt ttgcttatgc tttggcgatg gttctgacca
    39601 aagggacgcg ttacagcctg actttgtctg cttcggacga tatgccgtct gaagccggta
    39661 tgctgcccga ggcatattgg agcggtttgg aatgggtgcg ttttctgctg aacggcagtc
    39721 gggaatgccg ccgggtatat cgcaatcgtg aatggttgtg gcagcccgca agctgcaggc
    39781 tgaagatttc cgcaatgtcc gcaccgtcaa cgacgtggta caggcggtat tgaagattca
    39841 ggccggctaa tcgggtattt ctaattattt ttcagacggc atgtttttat cggatgccgt
    39901 ctgaaaacgt ttgtgcggat tcaaaatgaa acccggtttt ttcggacata tttttttgac
    39961 cgtattaagc attgcctatc ccgctttgtg
//
LOCUS       DAXDXW010000009        37388 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXDXW010000009 DAXDXW010000000
VERSION     DAXDXW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37388
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.009"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            71..2238
                     /gene="tdfF"
                     /locus_tag="ACFB64_000382"
                     /pseudo
     CDS             71..2238
                     /gene="tdfF"
                     /locus_tag="ACFB64_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent iron piracy receptor TdfF"
     gene            complement(2501..2824)
                     /locus_tag="ACFB64_000383"
     CDS             complement(2501..2824)
                     /locus_tag="ACFB64_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC1208817.1"
                     /translation="MDIVQLPSAALKAWMDEGRMFCLLDVRTDEEAAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLAEAGFENLYNLQGGIDAWAVEVDA
                     EMARY"
     gene            complement(2905..3561)
                     /locus_tag="ACFB64_000384"
     CDS             complement(2905..3561)
                     /locus_tag="ACFB64_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC1208818.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALEEIPRELFADESLQG
                     LAYADMELPLANGHKMLEPKIVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAERQKRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVTLVPEVL
                     KEQLKDGGRMAVIVGRRPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDAHPFDSFNF
                     "
     gene            complement(3664..4191)
                     /locus_tag="ACFB64_000385"
     CDS             complement(3664..4191)
                     /locus_tag="ACFB64_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC1208819.1"
                     /translation="MYANMGDMMHALHFSASDKAALYREVLPQIESVVADEADWVANL
                     ANTAAVLKEAFGWLWVGFYFVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGET
                     VVVKDVNAHPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDEADALYLGEL
                     AKILERRFEASSQAA"
     gene            4350..5123
                     /gene="tpiA"
                     /locus_tag="ACFB64_000386"
     CDS             4350..5123
                     /gene="tpiA"
                     /locus_tag="ACFB64_000386"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC1208820.1"
                     /translation="MYRQIGMWDQKWVIGNWKMNGRLQNNNALMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEERETGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            5130..5480
                     /gene="secG"
                     /locus_tag="ACFB64_000387"
     CDS             5130..5480
                     /gene="secG"
                     /locus_tag="ACFB64_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC1208821.1"
                     /translation="MEAFKTLIWIINIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNIRQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            5494..5579
                     /locus_tag="ACFB64_000388"
     tRNA            5494..5579
                     /locus_tag="ACFB64_000388"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5528..5530,aa:Leu,seq:gag)
     gene            5962..6083
                     /locus_tag="ACFB64_000389"
                     /pseudo
     CDS             5962..6083
                     /locus_tag="ACFB64_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            6469..6836
                     /locus_tag="ACFB64_000390"
                     /pseudo
     CDS             6469..6836
                     /locus_tag="ACFB64_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6833..7144
                     /locus_tag="ACFB64_000391"
     CDS             6833..7144
                     /locus_tag="ACFB64_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC1208822.1"
                     /translation="MKYKSGIFAAIHETMQGLHDIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            7254..7466
                     /locus_tag="ACFB64_000392"
     CDS             7254..7466
                     /locus_tag="ACFB64_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208823.1"
                     /translation="MPDRIKPSRNGGGADGIARQQGTAQTGAKPETSPTARHGDFLRP
                     LQGGIGFKRRGLEGKTGGAQNCLNAV"
     gene            complement(7916..8471)
                     /locus_tag="ACFB64_000393"
                     /pseudo
     CDS             complement(7916..8471)
                     /locus_tag="ACFB64_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8468..9276)
                     /locus_tag="ACFB64_000394"
                     /pseudo
     CDS             complement(8468..9276)
                     /locus_tag="ACFB64_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(9269..9439)
                     /locus_tag="ACFB64_000395"
                     /pseudo
     CDS             complement(9269..9439)
                     /locus_tag="ACFB64_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(9406..10197)
                     /locus_tag="ACFB64_000396"
     CDS             complement(9406..10197)
                     /locus_tag="ACFB64_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HFC1208824.1"
                     /translation="MNLFFDTQLGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKDWLLAVMKCIDQLTEEFTLSQMYEFENKLSIQ
                     FPQNNHIKDKIRQQLQILRDQNMIEFIGRGLYKKIDKLHPTPKAF"
     gene            complement(10426..13056)
                     /gene="leuS"
                     /locus_tag="ACFB64_000397"
     CDS             complement(10426..13056)
                     /gene="leuS"
                     /locus_tag="ACFB64_000397"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC1208825.1"
                     /translation="MQEHYQPAAIEPAAQKKWDDARISNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAVDWEREVATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETSCPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAESAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGNQDGLSKELKDLRHKLHSTTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLEAAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALANEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            13323..13839
                     /locus_tag="ACFB64_000398"
                     /pseudo
     CDS             13323..13839
                     /locus_tag="ACFB64_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
     gene            13846..14289
                     /locus_tag="ACFB64_000399"
     CDS             13846..14289
                     /locus_tag="ACFB64_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC1208826.1"
                     /translation="MPPRLLSGILCCLLTTAPVFAQGQPDAVSAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGTATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            14458..16515
                     /gene="ppk1"
                     /locus_tag="ACFB64_000400"
     CDS             14458..16515
                     /gene="ppk1"
                     /locus_tag="ACFB64_000400"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC1208827.1"
                     /translation="MTEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRPDNGKTPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDVQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFRLTDAELYQVKGPVNLVRLNAVPDLVNRPDLKFPPHTPGRLKALGKNSPIFD
                     LVRQSPILLHHPYQSFDPVVDMIREAAADPAVLAVKMTIYRTGTRSELVPALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIGRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     ATPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWDL
                     LRG"
     gene            complement(16664..17767)
                     /gene="dnaN"
                     /locus_tag="ACFB64_000401"
     CDS             complement(16664..17767)
                     /gene="dnaN"
                     /locus_tag="ACFB64_000401"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC1208828.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGRGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDNRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLGQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(17847..19403)
                     /gene="dnaA"
                     /locus_tag="ACFB64_000402"
     CDS             complement(17847..19403)
                     /gene="dnaA"
                     /locus_tag="ACFB64_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC1208829.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRPAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEKPVKPAASKTAADILAERMKNLPHEPRQAAGPASRPESAA
                     VAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAENPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            19695..19829
                     /gene="rpmH"
                     /locus_tag="ACFB64_000403"
     CDS             19695..19829
                     /gene="rpmH"
                     /locus_tag="ACFB64_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC1208830.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            19832..20197
                     /gene="rnpA"
                     /locus_tag="ACFB64_000404"
     CDS             19832..20197
                     /gene="rnpA"
                     /locus_tag="ACFB64_000404"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC1208831.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCGKQV"
     gene            20262..20483
                     /gene="yidD"
                     /locus_tag="ACFB64_000405"
     CDS             20262..20483
                     /gene="yidD"
                     /locus_tag="ACFB64_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC1208832.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGLRLAIKRIARCHPFGGHGHDPVP"
     gene            20656..22293
                     /gene="yidC"
                     /locus_tag="ACFB64_000406"
     CDS             20656..22293
                     /gene="yidC"
                     /locus_tag="ACFB64_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC1208833.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQKQAATA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFVLFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYTLNGDTVEVRLSAPETNGLKIDKVYTFTKD
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCHIDI
                     KRRSDKLYSASVSVPLAAIQAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQT
                     IKEKYGDDRMAQQQAMMQLYKDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(22374..23084)
                     /locus_tag="ACFB64_000407"
     CDS             complement(22374..23084)
                     /locus_tag="ACFB64_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC1208834.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLQHEQRAVVGLTDFVVEAEKTARA
                     HLKHLGITTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFNGYLPSEKNERIQSLNALEQR
                     SRQCGETQIFIETPYRNDALLADAVENLHPETRLCTATDLTLPTQLVVSKTVADWRRM
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(23146..23736)
                     /locus_tag="ACFB64_000408"
     CDS             complement(23146..23736)
                     /locus_tag="ACFB64_000408"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFC1208835.1"
                     /translation="MGLELPLVLGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPGALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMHRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCAAKSEGL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVDVL"
     gene            23884..24384
                     /locus_tag="ACFB64_000409"
     CDS             23884..24384
                     /locus_tag="ACFB64_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC1208836.1"
                     /translation="MSDPNLIDPEIFAAERQNLQGSFLLEELDERVSLHDYPADRRTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSDEESLDES
                     MLADEELEGILIEKELDVRALVEDQILMSLPFSPRHGHCGNTLPESANQDKPNPFAVL
                     AGLKSS"
     gene            24418..24597
                     /gene="rpmF"
                     /locus_tag="ACFB64_000410"
     CDS             24418..24597
                     /gene="rpmF"
                     /locus_tag="ACFB64_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC1208837.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALFVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            24772..25299
                     /locus_tag="ACFB64_000411"
     CDS             24772..25299
                     /locus_tag="ACFB64_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC1208838.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNAGVKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDSEGQVTEEVKKVQWLDPVPEVLRGKNVLVVDEVDDS
                     RVTMEFCLKELLKEDFDTVGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            25389..26435
                     /gene="plsX"
                     /locus_tag="ACFB64_000412"
     CDS             25389..26435
                     /gene="plsX"
                     /locus_tag="ACFB64_000412"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFC1208839.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALNAAGAPMERIDICHTTQVVGMDESPQSALKNKKYSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDKTGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALEAAQNETAASL"
     gene            26573..26824
                     /locus_tag="ACFB64_000413"
     CDS             26573..26824
                     /locus_tag="ACFB64_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC1208840.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFDILCTYIEDFDIRLTEQESREISFINKEFEIESTSD"
     gene            26851..27069
                     /locus_tag="ACFB64_000414"
                     /pseudo
     CDS             26851..27069
                     /locus_tag="ACFB64_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27220..27411
                     /locus_tag="ACFB64_000415"
                     /pseudo
     CDS             27220..27411
                     /locus_tag="ACFB64_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27441..27638
                     /locus_tag="ACFB64_000416"
     CDS             27441..27638
                     /locus_tag="ACFB64_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208841.1"
                     /translation="MPSEAAWAFRRHFLRLKQRIGNPAPPVHGNPHLKTICMPNHTPI
                     PVSVICTLPVLGNHRFFAYNS"
     gene            27671..28633
                     /locus_tag="ACFB64_000417"
     CDS             27671..28633
                     /locus_tag="ACFB64_000417"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC1208842.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALADAKLNINDIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAEQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDAGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            28680..29051
                     /locus_tag="ACFB64_000418"
     CDS             28680..29051
                     /locus_tag="ACFB64_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC1208843.1"
                     /translation="MKRQAFFKPMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWTGAYLGSRMFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            29077..30003
                     /gene="fabD"
                     /locus_tag="ACFB64_000419"
     CDS             29077..30003
                     /gene="fabD"
                     /locus_tag="ACFB64_000419"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC1208844.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEVGGKTPAAVAGHSLGEYTALVAAEA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICAESAQGEVVEAVNFN
                     SPGQVVIAGNAAAVGRTMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDAGKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQITAFIEAH"
     gene            30142..32007
                     /gene="msbA"
                     /locus_tag="ACFB64_000420"
     CDS             30142..32007
                     /gene="msbA"
                     /locus_tag="ACFB64_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC1208845.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAGIISTLQNWREQFTYMVWGTENKIWTVPL
                     FLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLMN
                     MLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRYY
                     RDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSKK
                     ITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKSL
                     ANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVKGRISFRNVDVEYRSDGIKAL
                     DNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLRA
                     QFALVSQDVFLFDDTLFENVRYSRPDAGESEVLSALQAANLQSLIDASPLGLHQPIGS
                     NGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGII
                     VAHRLTTVESADRIIVMDGGKIIEQGTHDQLMFQNGYYTMLRNISGKDTAAVQTA"
     gene            32107..33672
                     /gene="guaA"
                     /locus_tag="ACFB64_000421"
     CDS             32107..33672
                     /gene="guaA"
                     /locus_tag="ACFB64_000421"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC1208846.1"
                     /translation="MTQDKILILDFGSQVTRLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSGLTRGIQDDAPNTLDVWMSHGDKVSKLPDGFAVIGDTPSCPIAMMEN
                     TEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYYYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            33734..34480
                     /gene="fabG"
                     /locus_tag="ACFB64_000422"
     CDS             33734..34480
                     /gene="fabG"
                     /locus_tag="ACFB64_000422"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC1208847.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKIIGTATGESG
                     AAAISKRLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDNIMQVNLKSVFRASKAVLRGMMKQRSGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFAKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            34545..35030
                     /locus_tag="ACFB64_000423"
     CDS             34545..35030
                     /locus_tag="ACFB64_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFC1208848.1"
                     /translation="MNTHHHLPCPCGSGTSYAGCCRPLHLRQILPPTAEALMRSRYGA
                     YVLHLIDYIIATTVPAQQTFLDAAELMQWSREAEWLGLNVIAHRNLGKQHAQVEFEAY
                     FQDGGNRTVHHELSAFVKIAEQWYFIDPAVPLPTMKQPCICGSGKKFKACCGKYLQPV
                     A"
     gene            complement(35250..36044)
                     /locus_tag="ACFB64_000424"
     CDS             complement(35250..36044)
                     /locus_tag="ACFB64_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC1208849.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSRRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            36148..36873
                     /locus_tag="ACFB64_000425"
     CDS             36148..36873
                     /locus_tag="ACFB64_000425"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC1208850.1"
                     /translation="MPRFYLPENLSVGQTVDLPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHTARLTVLEKHRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSENTRLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAAIAAMQTLWGDFT"
     gene            complement(37069..>37388)
                     /locus_tag="ACFB64_000426"
     CDS             complement(37069..>37388)
                     /locus_tag="ACFB64_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HFC1208851.1"
                     /translation="ERIKAVDLMMFTYFFDKEGMPVYQVSPALCTQELHYAKFLSQNS
                     MLGSDLEKDREQGRRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKVYGRRVI
                     LFK"
BASE COUNT         9328 a        11189 c         8965 g         7906 t
ORIGIN      
        1 caacgccgta ccggtttaaa gttaatccgc tatacaaccc caccgcacac caacacacaa
       61 aggaaacccc atgacacgct tcaaatactc cctgcttttt gccgccctgc tacccgtgta
      121 cgcgcaggcc gatgtttctg tttcagacga ccccaaaccg caggaaagca ccgaattgcc
      181 gaccatcacc gttaccgccg accgcaccgc gagttccaac gacggctaca ccgtttccgg
      241 cacgcacacc ccgttcgggc tgcccatgac cctgcgcgaa atcccgcaga gcgtcagcgt
      301 catcacatcg caacaaatgc gcgaccaaaa catcaaaacg ctcgaccgcg ccctgttgca
      361 ggcgaccggc accagcccgg ctccgaccgc gcgggctaca actacctgtt cgcgcgcggc
      421 agccgcatcg ccaactacca aatcaacggc atccccgttg ccgacgcgct ggccgatacg
      481 ggcaatgcca acaccgccgc ctatgagcgc gtagaagtcg tgcgcggcgt ggcggggctg
      541 ccggacggca cgggcgagcc ttctgccacc gtcaatctgg tacgcaaaca cccgacccgc
      601 aagccattgt ttgaagtccg cgccgaagcc ggcaaccgca aacatttcgg gctgggcgcg
      661 gacgtatcgg gcagcctgaa cgccgaaggc acgctgcgcg gccgcctggt ttccaccttc
      721 ggacgcggcg actcgtggcg gcagctcgaa cgcagccgcg atgccgaact ctacggcatt
      781 ttggaatacg acatcgcacc gcaaacccgc gtccacgcag gcatggacta ccagcaggcg
      841 aaagaaaccg cagacgcgcc gctcagctac gccgtgtacg acagccaagg ttatgccacc
      901 gccttcggcc caaaagacaa ccccgccaca aattggtcga acagccgcaa ccgtgcgctc
      961 aacctgttcg ccggcataga acaccgcttc aaccaagact ggaaactcaa agccgaatac
     1021 gactacaccc gtagccgctt ccgccagccc tacggtgtgg caggcgtact ttccatcgac
     1081 cacagcactg ccgccaccga cctgattccc ggttattggc acgccgatcc gcgcacccac
     1141 agcgccagca tgtcattgac cggcaaatac cgcctgttcg gccgcgagca cgatttaatc
     1201 gcgggtatca acggctacaa atacgccggc aacaaatacg gcgaacgcag catcattccc
     1261 aacgccattc ccaacgccta cgaattttcc cgcacgggcg cctatccgca gccatcatcg
     1321 tttgcccaaa ccatcccgca atacgacacc aggcggcaaa tcggcggcta tctcgccacc
     1381 cgtttccgcg ccgccgacaa cctttcgctg atactcggcg gcagatacag ccgctaccgc
     1441 gcaggcagct acaacagccg cacacaaggc atgacctatg tgtccgccaa ccgtttcacc
     1501 ccctacacag gcatcgtgtt cgatctgacc ggcaacctgt cgctttacgg ctcgtacagc
     1561 agcctgttcg tcccgcaatt gcaaaaagac gaacacggca gctacctgaa acccgtaacc
     1621 ggcaacaatc tggaagccga catcaaaggc gaatggcttg aagggcgtct gaacgcatcc
     1681 gccgccgtgt accgcgcccg taaaaacaac ctcgccaccg cagcaggacg cgaccagagc
     1741 ggcaacacct actatcgcgc cgccaaccaa gccaaaaccc acggctggga aatcgaagtc
     1801 ggcggccgca tcacgcccga atggcagata caggcaggct acagccaaag caaaccccgc
     1861 gaccaagacg gcagccgcct gaaccccgac agcgtacccg aacgcagctt caaactcttc
     1921 accgcctacc acttagcccc cgaagccccc agcggccgga ccatcggtgc gggtgtgcgc
     1981 cggcagggcg aaacccacac cgacccagcc gcgctccgca tccccaaccc cgccgccaaa
     2041 gcccgcgccg tcgccaacag ccgccagaaa gcctacgccg tcgccgacat catggcgcgt
     2101 taccgcttca atccgcgcac cgaactgtcg ctgaacgtgg acaacctgtt caacaaacac
     2161 taccgcaccc agcccgaccg ccacagctac ggcgcactgc ggacagtgaa cgcggcgttt
     2221 acctatcggt ttaaataagg tcgtctgaaa acggagtttc tgcgaagcta aaagcagcct
     2281 gcacattgaa aatgccgccc aagcaaacct gcagtttgcc gccctctccc tagccttctc
     2341 ccacggggcg agggaattgg ggtgcaggct gcctttaagg ttcaggcaaa ttttaacttc
     2401 gttgatactg cgctttagct tcgcagaagc tgcactttca gacgaccttt atggaacacc
     2461 agaggtacac gcatttaagg aatgccgtct gaagccgcct tcaataccgc gccatttccg
     2521 cgtcaacctc aaccgcccac gcgtcgatgc cgccctgaag gttgtacagg ttttcaaaac
     2581 ccgcctccgc caaatacatc gccgtatgca ggctgcggat accgtgatgg caatacacca
     2641 caagcggcac atcgtccggc aactcgtttt gccgcagcgg aatcagattc atcgggatat
     2701 gcagcgcatt tggcagcgaa caaaccgccg cttcttcatc cgtacgcacg tccaacaaac
     2761 aaaacatccg cccttcgtcc atccacgcct tcaatgccgc cgacggaagt tgcacaatat
     2821 ccatcacaaa ccccccgaaa aaaaccaagc aaaatgccgt ctgaagcccc aaacccgctt
     2881 tcagacggca tcacaatccg aacatcaaaa attaaaactg tcgaaaggat gggcatcctt
     2941 gtcgtccaaa tgcgccacca aagtatcgaa cagcaccttc tcttcaaaca catcgcccct
     3001 gcgcgtaatc aaaagcgcgc gctgcaccgg cctgcgtccc acaataaccg ccatacgccc
     3061 gccgtccttc aactgttcct ttaacacttc aggcacaagg gttaccgcgc cgccgacgta
     3121 aaccgcatca aacggcgcac ctgcggaaag ttcggtcaac ccgttatttt gcacataatc
     3181 gatgttttcc aaagacaagc cgtccaaaac cgctttggcg cgcttttgcc gttcggcatc
     3241 gatgtcgtcc gaaaccacac ggccagccag ttttgccaac agagccgtcg catagcccga
     3301 acccgtgccg atttccaaaa ccgtatcgtt tttcgtcagc ttcaagccct gcgccagccg
     3361 cgccacgatt ttcggctcga gcatcttatg accgttggca agcggcagct ccatatccgc
     3421 atacgccaaa ccctgcaaag actcatccgc gaaaagctcg cgcggaatct cctccaaagc
     3481 gtccaacacg tcaaaatcca atacatccca aggacggatc tgctgttcga ccatattgaa
     3541 ccgcgctttt tcaaaatcca tttagtgctc cgtcaaataa ttgttttatc aatggcaggc
     3601 attataaaac cacactaccg ccgcgccaac ttcggcacgc gaagcccgcc cgttttgcca
     3661 gtctcaagcc gcctgactcg aagcctcaaa ccgcctctcc aaaatcttcg ccagttcgcc
     3721 caaatacaaa gcatctgcct catcaaactg cgccaaatgt tcgctgtccg cgtccaacac
     3781 gccgatacag cggccgtctg aaaacagcgg cacgacaatc tccgaacgcg acaaagacga
     3841 acaggcaata tggtcgggat gcgcgttcac atccttaaca accaccgttt cacccttcgc
     3901 ccaagcctga ccgcacaccc cgcgcccgaa cggaatccgc gtacacgcca aaggtccctg
     3961 aaacggtgcc aaaaccaatt cgtccgaacg cgtatcgaca aaataaaaac ccacccacaa
     4021 ccagccgaac gcctccttca aaaccgccgc cgtgttcgcc aaattcgcca cccaatccgc
     4081 ctcgtccgcc accacagact caatctgcgg caacacctca cgataaagcg cggccttgtc
     4141 cgaagccgaa aaatgaagcg cgtgcatcat atctcccata tttgcataca tatcaggcag
     4201 ccattataaa acatcctgcc cgaaacaaca ttccaaaccg cccccgcagc cgcccgcccc
     4261 aagttgcgaa cccgccgcat atccactaaa cttcacattg caacgcgcca cacgcggcag
     4321 acagaaaaac acgacacgga gcaaaaaaga tgtatcgcca aatcggaatg tgggatcaaa
     4381 aatgggtcat cggcaactgg aaaatgaacg gccggctcca aaacaacaac gcactgatgc
     4441 accgcttccg catcctcccc accgccgaac gcgtcctcat cggactcgcc gccccgaccg
     4501 tttacctgct gcaactgcac aacgccatgc aaatcgtctt aaacaaccgc atcctcacct
     4561 gcgcccaaga cgtgagccgc ttccccgaca acggcgcgta caccggcgaa gtgtccgccg
     4621 aaatgctcgc cgacaccgga acagacatcg tcctcatcgg acactccgaa cgcagcctct
     4681 atttcggcga aaaaaacgaa atccaacgcc gcaaaatgga aaacgtcctc aacgtcggac
     4741 tcatcccatt attgtgcgtc ggcgaaagcc tcgaagagcg cgaaaccggc aaagaacacg
     4801 aagtcatcgc ccatcagctt tccatcctgc aagggctgga taccaaaaac atcgccgtcg
     4861 cctacgaacc cgtctgggca atcggcacag gcaaagtcgc caccgtcgaa cagattgccg
     4921 atatgcacgc attcatctac aaagaaatct tgtctttgtg cggaagcgat gttaaaatcc
     4981 gcgtccttta cggcggaagt gtgaaagcgg acaacgcggc cgacatcttc gcagtacctt
     5041 atgtggacgg cgcactcgtc ggcggcgcgt cattgtcgta cgactccttt accgccatca
     5101 tcagtgccgc acaaaatgcg tagaaaaata tggaagcctt caaaacccta atttggatta
     5161 ttaatattat ttccgctttg gccgtcatcg tgttagtatt gctccaacac ggcaaaggcg
     5221 cggatgccgg cgcgaccttc ggatcgggaa gcggcagcgc gcaaggcgta ttcggctctg
     5281 ccggcaacgc caacttcctc agccgctcga ccgccgttgc agcaacattt ttcttcgcaa
     5341 cctgcatggc tatggtgtat attcacaccc acacgacaaa acacggtttg gacttcagca
     5401 acatacgaca gactcagcaa gcacccaaac ccgtaagcaa taccgaacct tctgcccctg
     5461 ttcctcagca gcagaaataa cagtttttca aatgccgaca tggtgaaatt ggtagacacg
     5521 ctatcttgag ggggtagtgg ccgtaggctg tgcgagttca aatctcgctg tcggcaccaa
     5581 cacacaaaaa cgcttagaaa ttctaggcgt tttttttcgt ttaatcctgt ctgatgccat
     5641 caaccaatcc caatcaatac aaggatttca ggctgattgt tatcctgccg tccccttcct
     5701 gactgtgcaa tcccgtccaa tccgccctaa ttgaactaac ctgatttttt acggtatccg
     5761 aaaaataccg caaaaataaa gctgaacgac caccaaatca ggaatgccaa gcggaaaaga
     5821 gcttgcgggg aatactgacg aaccgtaggg aacgaggggg aaaccgtcca agatgcagag
     5881 cggttttttt gggtttttgg aaaaaaccta tactaggaag cgataccctt agttgttacc
     5941 ttgttaccgg ggaaaagtta gataaataag tatatgaaat atagtgaatt aaatttaaat
     6001 cgggacaagg cggcgaaccg cagacagtac aaatagtacg gcaaggcgag gcaacgccgt
     6061 accggtttaa atttaattca ctataaaaat aagaaaaaga taaaaaattg gtaacaaatg
     6121 cggtaacaaa tggtaacgaa tcggtaacaa cttttggggt tttccggttt tttaccgtct
     6181 tggcagtggg agcgtagcgg aatgaaaagc caaaacgcac ggaaccgcgc ctattttgag
     6241 caggaatggc ggttaaaccg gttggttata ttgcggggca taggaagaca gcgaaacgcg
     6301 ctaaaaacgc aatttgagcc attaaaagcg attgattaaa aaataatcaa attagccgcc
     6361 ggtgtttcag gcggcataaa cggagaaatt gcagggtata aaaaggcggc ttgccgtgtt
     6421 gcctgtcttt ggtataatcc ccagtatcac taagtaaacg gttgcacaat gcggatattc
     6481 aaaaaccaat ggatagtgaa atttgccaag aagcacaaaa tcagtgattc agagctgata
     6541 gaggcggtag agcgggcaga taacgggctg atagatgcag atttgggcgg cgcgtgatta
     6601 agcagcgcat agcaaggcaa gggcaaggca gaagcggcgg ttatcgcagt ctgatatttt
     6661 tcaggcatgg agaacgggcg tttttcatga ccgcctttgc caagaacgac cgcgaaaaca
     6721 tcactgataa agaacttaca gaattaaaga aaaccgccgc catcatcttg gcaatgacag
     6781 aaaccgacat agagcaagcc aagtcaaacg gcactttcac ggagatacag ccatgaaata
     6841 taaaagcggg attttcgcag caatacacga aaccatgcag ggattgcacg acatcggcgc
     6901 aatcgacaaa aagactatgc gcggctttga caagtcctgc ctgaccgaaa tcaagccgtt
     6961 gagcggcggg gacatcaagg caatcaggga gaaggaggca ctatcgcaag ccgctttcgc
     7021 catctatctc aacgtgggga aaaatcacgt ttcggcttgg gagcggggcg ttaaaaagcc
     7081 gagcggcgcg gcgttgaagc tgctgaccat cgtcaaaaac aagggcatcg aagccattgc
     7141 gtagccgact tggcaaacgg caaaatcagc aagttcacaa tagacgcgct gctgaatatg
     7201 cctgccaaaa caggcaagac cgccgaactg aatatcaggg cgtagccgca taaatgcccg
     7261 accgcatcaa accaagccga aacggcggcg gtgcagacgg catagcccga cagcaaggca
     7321 cggcgcagac gggcgcgaaa cccgaaacat ccccgaccgc gaggcacggg gattttttgc
     7381 gcccgttgca gggggggatt ggatttaagc ggcgcgggct tgaaggcaaa acgggcgggg
     7441 cgcagaactg tttaaatgcc gtctgaatct caaacgattt cagacggcat tttgaaacaa
     7501 cggctcaaat tctcgatccc cttcccttaa cgccgacgtt ttttattaac gcgcccctta
     7561 tttctgacac tttgctcata aaccggcata acggtcggca acaaccgttt tagattttct
     7621 atacaggcat tgtttacagc atcagcataa gacaccgtac tctggaatac cgcctaaacc
     7681 ctgcggatag ttttggatgt ggtatcgaca ttgtcggcag acttgtttaa ctcgaccgtt
     7741 ttcaaataat ctttgacaga tgctgcattc agtgtcgcgg aatccgcgta ttttgaacaa
     7801 aaggtcgggc tactttatca caatagcaga aagatggaaa acggcgggga ttggtgaaag
     7861 gtcgtctgaa atgatattca gacgaccttg aacggttgtg ttttaaatat ggtatttatt
     7921 catttgtttc accatcaggc agtccaaacc accttaaggg aatcgtgtta aattggtaat
     7981 ggcagttaac actgataata taatctaaaa catctttaat attccggttt tgtaaaccaa
     8041 tcattcaaaa catagacata tccaacccgc caacctaaag agtgaactaa ctttgtatat
     8101 tgttttctct taaaatcgca agtttgtaat ttctcatcta ctgaaccggg agtttgttgg
     8161 aatttgatct caataataaa taaggtatca cggatgatga taaataagcc attatcgggc
     8221 agtaatcttt tagacagatg gtctttccaa ttaatattgt attccagtct ttccaaaaat
     8281 cggtaaaact catatttttt gaaacaataa gccttcaatt ctccattaaa ccaaacttca
     8341 taacccgcat tatcatctgt ttttcttaag tcgtacccgg gaatttcttc aaacaacttt
     8401 cgtaagtctg tacgttcttc gaatctgagt cctgtttgtg tccttgcacc gccgattccg
     8461 ccaataatca tttttctaac cttttctttg ctaattcaaa aaaatcttct tccagttcgc
     8521 aaccgcaaaa tctccgacca tgttttaagg cggcaacgcc tgttgtgccg ctgcccataa
     8581 aagggtcaaa aattaaatct ccgatatttg aagccgatag tatgcagcgt tcaagtaagg
     8641 ggagtggttt ttgtgtcgga tgtttgccga atgttttttc ggttttattt ggtggaggaa
     8701 atgtccaaac acatttcatc tgtttgccat cattttgtgt tttcatggtt tcataatgaa
     8761 acgtgtgttt agctttttta tttttctttg cctataagat tgtttctgtc gaatgggtaa
     8821 agaaacggca ggacaaatta gggggaggat tgggtttttc ccaagtaata ttgttcaaaa
     8881 tatggtagcc gacggtttgc atcaggtagc cgattaaata gatattatga aatgtgccgc
     8941 aaacccaaat tgtgccgttt ggttttaata aggcgtaaca cagtcggagc cattcttcgt
     9001 aaaattccaa atctgccgcc attcctttgg atttatccca gttgccctta ttgacggaaa
     9061 ccatttgccc gttttgacaa ctgaaaccgt cattggaaag aaagtaaggc gggtctgcga
     9121 aaatcatatc aaaacagccg tttggatgtt tttccaatat cttccgcatg acgttaaggg
     9181 aattttcgtt gtagaggaca aggtcgtctg aaacgatttt ttcaaacgac tttgataaat
     9241 ggcagcttct tcagccggtt tgtcaaaatc aatcataaat tgccaataat tctccgactt
     9301 ttttacgact gctgcccttt gcggcaatat tgcgttgtac ttcaaccgtt tttaaaatgg
     9361 cagaagaata aatatttctc gtaaattcag tatcatgatt tgaaatcaaa acgccttggg
     9421 agttgggtgc aatttgtcga tttttttgta aagtccgcga ccaatgaatt cgatcatatt
     9481 ttgatcacgc aaaatttgca actgttggcg gattttgtct ttgatatggt tgttttgggg
     9541 aaattggatg gatagtttgt tttcaaattc atacatttgc gacaatgtga attcttcggt
     9601 gagttggtcg atacatttca taacagccaa aagccagtct ttgcgctccg cattttggtt
     9661 gcgtaaaaac aggttggatt gccatttttt cagaacggtt tcgggttcga taatgcggga
     9721 atcgtctatt aagaatattt ttccgctttc aggcaaaggg gcgagattga tggaacacat
     9781 aatgtggtgc ggtcggtttt taatgccttt atttctggga ataatcatat ccggcgtgat
     9841 gaaatgtttg ggtacaagca ccaattgccg tatggagtaa tccgcttttt tatatgcaag
     9901 aaagaaaaag ttggggttgg tatctgaccg gatgcgctcc aacatagtgc gatatgcacc
     9961 gtcaggcacg ctgttgccta tggttttttg atttttactc tttaattcat attgctcgtg
    10021 gcaatttggg caaaagaggt ctgcaacagg tttgttattg gcaaatctct gcatcggctt
    10081 gcttccgcaa caggggcaat agccgttttt ttccagccaa gcctcgctca ttacacggat
    10141 tttgtgggtt gctttatttt gttgctttcc caattgggta tcgaaaaata aattcatatt
    10201 ttggattttg agattaattt ttagtagatt aacaaaaatc aggacaagga gacgaagccg
    10261 cagacagcag ggcaaggcgg ggcaacgccg taccggttta aagttaatcc gctatatttg
    10321 aggtctgtct tgcagacaac ccaatctgcc ttaaaaaggc cgtctgaaac cctgtttcca
    10381 agtttcagac ggcctttatc cgtatggcta aaccttaaaa gcggtttaga cgacgatgtt
    10441 caccagtctg cccggtacga cgatgatttt cttcgcaggc ttgccttcca tgaatttcac
    10501 tgcgccttcg ttggcgagtg cggcggcttc gaggtcggct ttggaggcgt cggcggcgac
    10561 ggtgattttg ccgcgcagtt tgccgttgac ttgaaccatc acttcgattt cggatttgac
    10621 cagggcggct tcgtcgactg tcggccagcc tgcttcccac agtttcgcgc cgttcaattc
    10681 gctccacagg gtttcgcaga tgtgcggcac gatgggccac aacaggcgta cggcggcttc
    10741 caatacttct tgggcgacgg cgcggccttg ttcgctgccg gtgtcggttt tgtcgtattg
    10801 gttgagcagt tccatcacgg cggcgatggc ggtgttgaac tgctggcggc ggccgtagtc
    10861 gtcgctgact ttggcggtgg tggaatgcag tttgtggcgc aggtctttga gttctttaga
    10921 caaaccgtct tggttgcctg caaatgcttt gaccgcgccg ccttgcttca ggtattcgta
    10981 aacggtacgc cacagacggc gcaggaagcg gtgtgcacct tcgacgccgc tgtcgctcca
    11041 ttcgagggac tgttcgggcg gtgcggcgaa catcatgaac aggcgggcgg tgtccgcgcc
    11101 gtaggcgtta atcagttctt gcggatcgac gccgttgttt ttggatttgg acattttttc
    11161 cgtgccgctg atgacgacgg gcagtccgtc ggctttgagg acggcggaaa cggggcggcc
    11221 tttgtcatcg aaagtcagct cgacatcggc ggggttgatc cagtctttgc cgcctttgtc
    11281 gttttcgcgg tagtaggttt cgcagacgac cataccttgc gtgagcaggc gttcaaacgg
    11341 ttcgtcaaca ttgaccaaac cttcgtcgcg catcagtttg gtgaagaagc gcgcgtacag
    11401 gaggtgcaaa atcgcgtgtt cgatgccgcc gatgtattgg tcgaccgcgc cccagtattt
    11461 cgcggattct gccgatacca tgccgtctga aaacttgggc gacatatagc ggaagaagta
    11521 ccagctcgac tccatgaagg tgtccatggt gtcggtttcg cgtttcgccg cgccgccgca
    11581 gcacgggcag gaagtttcgt aaaactcggg catttttgcc agcggcgaac ccataccgtc
    11641 gggtacgacg ttttcaggca ggacgacggg cagttggtcg gcagggacgg gaacgtctcc
    11701 gcatttttcg caatggacga tgggaatcgg gcagccccag tagcgttggc gcgaaatgcc
    11761 ccagtcgcgc aggcggtatt gggttttcgg ttcgcccgca ccttggcttt gcagcttggc
    11821 ggcaacggca tcaaatgccg tctgaaaatc caagccgtcc aagtcgccgc tgttgaccaa
    11881 tacgccgttt tctttgtcgc cgtaccattc ttgccatcgg tttgcgtcga atgcgttgtc
    11941 gccgacggca atgacttgtt ttttcggcag attgtatttg gcggcaaact cgaaatcgcg
    12001 ttcgtcgtgc gccggaacag ccatcaccgc gccgtcgccg tagccccaca agacatagtt
    12061 ggcaatccac acttccagct tgtcgccgtt gagcgggttg acgacgtagc ggccggtcgg
    12121 cacgcctttt ttctccatcg tcgccatatc ggcttcggca accgaaccgg ctttgcattc
    12181 ggcgataaat gcctgcaatt cgggtttgtc ggcggctgcg gctgttgcca gcggatgttc
    12241 ggcggcaaca gcgacataag tcgcacccat cagcgtgtcg gggcgggtgg tataaacttg
    12301 caggaatttc gcgtaatcgc cttccaaacc ttgcttgctg tcgtctgaaa cggcgaagcg
    12361 cacggtcatg ccgcgagatt tgccgatcca gttgcgctgc atggttttga cttgttccgg
    12421 ccagtgttcc agcttgtcca aatcgttgag cagctcttcg gcgtaatccg tgattttgaa
    12481 gtaatacatc gggatttcgc gtttttcgat caacgcgccc gaacgccagc cgcgtccgtc
    12541 gatgacttgc tcgttggcaa ggacggtttg gtcgaccggg tcccagttca ccgtgccgtt
    12601 tttgcgatag acgatgcctt tttcaaacag cttggtaaac agccattgtt cccagcggta
    12661 gtattcgggt ttgcaggtgg cgacttcgcg ttcccagtca accgcaaaac ccaggctttt
    12721 gagctgggtt ttcatgtatt cgatgttgtc gtaggtccaa gcggcggggg cgacgttgtt
    12781 tttcatcgcc gcattttccg ccggcatgcc gaacgcgtcc caacccatag gctgcataac
    12841 gttgaagccg tttaaaagtt tgaagcggct caatacgtcg ccgatggtgt agttgcgtac
    12901 atgccccata tgcagcttgc cgctagggta agggaacatc gaaaggcaat aatatttggg
    12961 tttggaagcg tcttcggaga cgttggaaat acgggcgtcg tcccattttt tctgcgccgc
    13021 aggctcgatg gcggcgggct ggtaatgttc ttgcatggtc attttgtttt tcgcttgaaa
    13081 acgttaggaa tttaaaggag gaattataac aggttgccga tgtcggcgaa tcggcaaatt
    13141 gccggcagga tgcgtaaatt cgcacgcgca tcattccgta tgccgtacaa atacaccgcg
    13201 tttactgata cgcacgtttt ttatgctaat attacgaacc aaaatcaaac gtttaaaact
    13261 ctcctgatgc ggctcttccg aacaaaaggc agacgggcat cgggtaaaaa aggattctgc
    13321 atatgaaaat caaacaaatc gtcaaaccga gcttggcagt attggcggca ggcattctgt
    13381 ctgcctgcgc aaccataaag caacgtcaaa gccgacggca cgaccgacaa tccggttttc
    13441 ccggaaccct attccgtaac gctcgacaac aagcgcggca cattcccgac ttatgacgaa
    13501 ctggatcaga tgcgccccgg tctgaccaaa gacgacatct acaaaatccc gggccgcccg
    13561 cattacgacg aaggtatgta cggcgtgcgc gaatgggatt acctgttcca cttccacacc
    13621 ccgggcgtgg gtatcgaccc tgaaaacact tccggcgtgg aaggcgttac tacctgccaa
    13681 tacaaagtga ttttcgataa agacaaattt gcccgcagct tctactggaa ccccgtcttc
    13741 ccgaaagatg ccgtctgccc gccgcccgca cccaaagccg agccgcaagt catcatccgc
    13801 gaaatcgtgc cggcaaaacc gaaacgtatc cgccaataag ccgctatgcc gccccgcctg
    13861 ctttcaggga tattgtgctg cctgctgacg actgcccccg tattcgcaca ggggcagccg
    13921 gatgcggtca gtgcctatat tcaaaagaaa aaagtcatcg tcgacacatc caaggcggaa
    13981 ctctgttttg ccgacgaccg tcagtgccac cccgtcctca tcggcactgc cacacccaag
    14041 gggacgttcg gactgacgct gaacagtacc gacaagcccg gttacggcgg cgaagtcatc
    14101 ggtttcaagc aggagggcga ttttcttttc gccctgcacc gcgtttggaa tcagataccg
    14161 tccgaaaggc gaaacgaacg catcgcctcc ccgtccgtgt ccgacaggat tatgaccaac
    14221 ggctgcatca acgtcagcga tgcggtgtac gaaaaactgc gtcattattt tgtgttggaa
    14281 gtgatttgaa acagacggat accgcacgtg ccggtatccg ttttcacatt gccccgatgc
    14341 ctgaaacaga ccgtccgtca cgtcatgccg cctgaaaccg gcgcagatgc cgccaagcct
    14401 tcagacggca ttgtccgccc gctccgaccg aacaacaacc atctttggga gaaccttatg
    14461 accgaacaaa accgcatcct ctgccgcgaa ctgagcctgc tggcattcaa ccgccgcgtg
    14521 ttggcacagg cggaagacaa aaacgtcccc cttttggaac gcctgcgctt cctgtgcatc
    14581 gtgtcgtcca atcttgacga gttttttgaa gtccgcatgg cgtggctcaa gcgtgaaaac
    14641 aagctgcacc cccggcgcag gccggacaac ggcaaaacgc cgtctgaaac catcgccgac
    14701 gttaccgaag cggcgcgctc cctgatacgg caccagtacg acctgttcaa caacgtcctt
    14761 cagcccgagt tggcgcggga aagcatccat ttttaccgcc gccgcaattg gacgggcacg
    14821 cagaaaaaat ggatcgaaga ctatttcgac cgcgaactgc tgccgatcct gacccccatc
    14881 ggactcgacc cttcccaccc tttcccgcgc ccgctgaaca aatcgctcaa cttcgccgta
    14941 gaactcgacg gcacggacgc gttcggcagg ccttcgggta tggcgattgt ccaagcaccg
    15001 cgcatcctgc cgcgcgttgt tcccctgccg tccgaactgt gtggcggcgg acacggcttc
    15061 gtcttcctgt cgtccatcct ccacgcccac gtcggcaaac tcttcccggg catgaacgtc
    15121 aaaggctgcc accagttccg cctcacgcgc gacagcgact tgaccgttga cgaagaagac
    15181 gtacaaaacc tccgcgccgc cattcaaaac gaactgcacg accgcgaata cggcgacggc
    15241 gtgcggctcg aagtcgccga cacctgtccc gcctacatcc gcgactttct gctcgcgcaa
    15301 ttcaggctga ccgacgccga actctatcag gtcaaaggcc cggtcaacct cgtccgcctc
    15361 aacgccgtcc ccgacctagt caaccgcccc gatttgaaat ttccgccgca cacgccgggc
    15421 cgtctgaaag ccttgggcaa aaacagcccg atcttcgatt tggtgcgcca atcgcccatc
    15481 ctgctgcacc acccctacca atctttcgat cccgtggtcg atatgatacg cgaagccgcc
    15541 gccgatcccg ccgttcttgc cgtcaaaatg acgatttacc gcaccggcac gcgctccgaa
    15601 ctcgtccccg ccctgatgaa ggcggcactc gccggcaaac aagtaaccgt cgtcgtcgaa
    15661 ctgatggcgc gttttgacga agccaacaac gtcaactggg cgaagcagct cgaagaggcg
    15721 ggagcgcacg tcgtgtacgg cgtgttcggc tacaaagtcc acgccaaaat ggcactggtc
    15781 atccgccgcg aagacggcgt gctcaagcgt tacgcccacc tcggcacggg caactaccac
    15841 caaggcacat cgcgcatcta caccgacttc ggcctcatta ccgccgacga acaaatcacc
    15901 gccgacgtga acacattatt tatggaaatc acaggcttgg gcaaacccgg gcggctgaac
    15961 aaactctacc aaagtccgtt taccctgcac aaaatggtta tcggccgcat cgctcgcgaa
    16021 accgaacacg caaaagccgg caaaccggcg cggattaccg ccaagatgaa ttcgctcatc
    16081 gaaccgactg tcatagaagc cctgtatcgg gcaagcgcgg caggcgtgca aatcgatttg
    16141 attgtgcgcg gtatgtgtac cttgcgcccg ggtgtaaaag gcttgtccga aaacatccgc
    16201 gtccgctcca tcgtcggcag gcagctcgaa cacgcgcgcg tatattgttt ccacaacaac
    16261 ggcgcggacg acacctttat ttccagcgcg gattggatgg ggcgcaattt cttccgccgc
    16321 atcgaaaccg ccaccccgat taccgcgccc gaactcaaaa aacgcgtcat ccgcgaaggt
    16381 ttggaaatgg cactggcaga caatacccac gcgtggctga tgcagcccga cggcggctat
    16441 atccgcgccg cgcccgccga aggcgaaagc gaagccgacc tgcaaaacga tttatgggat
    16501 ttgctccgag gctgacccgc acaagcactt cccccatacg ctcccaaccc caatccgccg
    16561 cattccagac ggcattgcct ttccggcagc cgaacaaaac cccgaatccg tctgcacggt
    16621 tcggggcata tctccaatac gggcatcgtg ttccggaaaa ccgtcaaatc cgcatcggca
    16681 tcacaatata tttgaaattc ggattgttcg gcacggtaaa cagcgtcgag cggttggcgt
    16741 cgccgaaggc aagctgcata tcgtcggaat ggatgttgcg caacacgtcc atcaaatagc
    16801 cgatattgaa accgacttcg agttcgccgc cctgataggc gatttcgatt tcttcgcgcg
    16861 cttcttcctg ctcgttgttg ctgcacacga cgctcaacag gccgggttgc aggaacaggc
    16921 gcgcgccacg gaatttttcg ttggcaagaa tggacacacg ttccagcgcg cccaaaagtt
    16981 cggcacggga caacacgaaa atcttgtcgt tgtccaaagg aatcacgcgg ttgaaatcgg
    17041 ggaatttgcc gtcgatgacc ttgctgacga tggtcgtgcc gttgcattgg aaacgcacct
    17101 gcttgtccag cagctcgatt tgaatcggat cgtcggggtt gttcaacagt ttgaacagtt
    17161 ccagcaccgt tttgcgcggc aaaatcactt cggcgcgcgg caaatccgca tcaatcgcgc
    17221 aggctgcata ggcaaggcgg tgtccgtcgg tcgccacaag gcgcaactgg ctgccctcaa
    17281 cctgcatcag cagaccgttg agataatagc ggatgtcctg caccgccatg ctgtactgca
    17341 cttgcgacag catggttttg aaacgctcct gccccagcga gaaagtcgcg ctgatgtcct
    17401 cgccgacatt catcatcgga aaatcggcgg caggcagggt ttgcagggca aaacgcgatt
    17461 tgcccgcctt cagcgtcaga cggttgtcgt cccaatccag cgacaccagc gcaccggcag
    17521 gcagcgcgcg caaaatgtcc tgaaatttct tggcattggt agtgatgcgg aagtcgcccg
    17581 cacctccctc gggacccgcg gtgtcgattt ggatttccaa atcggttgcc aagagtttgg
    17641 tctgaccgcc cctgccctca atcagcacat tggacaggat gggcagggtg tgtcggcgtt
    17701 cgacgatgcc ggtaacggct tgcagcggct tgagcaggct gtcgcgctcg gcttgtaaaa
    17761 tcaacatttt cgctccttaa atcggttgga tttcctaccc ttcggacggc atggtcaatg
    17821 tcatgccgtc tgaaaggcgt gtccggtcag ttttgaatca gaatcagcag tttctcgtaa
    17881 tcctgcgcca actcggggtc ttcctcgcgc agtttcgcca ccgccctgat gccgtgcatg
    17941 acggtcgtat ggtcgcgtcc gccgaacgaa tcgccgatag acggcaggct caaagtggtc
    18001 aattctttgg tcaggctcat agcaacctga cgcggacggg caatgttgcg cgtgcgtttt
    18061 ttgccgagta cgtcgctgat tttgatgcgg taatatttcg ccaccgcatc gatgatgatg
    18121 tcggcggtga tgactttgtg cttctcggca ataatgtcct gcaaagcggt acgcgccaaa
    18181 tcgatgtcga tgacgggacg gttcataaag cggctgctcg ctccgacacg gttgaacgcg
    18241 ccttccagtt cgcgcacgtt ggaacggatc agattggcaa tgaacagcgc ggcttcgtct
    18301 tcgatactga tgcccgccgc ttccgccttt ttctgcaaaa tggcgatacg catttccaat
    18361 tcgggcggct cgagttccaa agtcagcccc cacgaaaagc gggatttgag gcggtcgtcc
    18421 ataccttcga ttttggcggg taaaacatcg caagtgagga tgagctgttt tttctcattg
    18481 tgaaaatggt tgtacagata gaaaaattct tccatcgtac ggtcttttcc tttgatgaac
    18541 tgaatatcgt cgataatcag caggtcgtat tgcttgtatt gctgcttgaa cacgtcgtag
    18601 gtattgttgc gaaccgcctt cataaagctg cggatgtagt cgtccgaatg catatagcgc
    18661 actttggcat cgggacggtt tttcaacagc tcgttgccga ccgcctgcac aaggtgggtt
    18721 ttgcccaaac ccgtgctgcc gtacaggaag aacgggttgt aactctgccc cgggttttcc
    18781 gcaatcgcct gcgccgcagc cgccgcaagg cggttgccct taccttctac caacgtatca
    18841 aacgtgtaat ccggagacag gttggtttgt tcgtaacgcg cttcttccgc atcacgctgc
    18901 gcatccgtcc gcgctttggc aactgccgcc gattccggcc gggaagcagg cccggcagcc
    18961 tgacgcggct cgtgcggcag gtttttcata cgttccgcca aaatatccgc cgccgttttc
    19021 gacgcagcgg gtttgacagg cttttcagac ggcagctcgt ccaacagaac ctcctgcacg
    19081 ggcatctcct ccgaccccgc gtgcaaggac ggctcggcag gttcgacagc accttcaacc
    19141 gccgccatct cataacgcac gccttctccc ggtttgaata cgaaggcggg acggccggca
    19201 gccaactctt ccctcaccgc ttctattttt ccggcaaact ggctcttgag catattgcag
    19261 gcaaactggt tcttgccgta caccacccat acgccaccct cctcaccaac cgtaaggggc
    19321 gcaatccatt gcgcaaactg cccgtgaggc aacatatcgt gaagacggcg gaggcacagc
    19381 ggccaaaact ctgctaatgt catggatagg ctcgaatcgg taaaaatgaa atcgaaaaca
    19441 aagaaaatat aatattttca aaaagaaaac aaatctgttg aatacgcgtc ggttcaaaac
    19501 gcagcgaacc gattataccg actcgccgat attttatcca caacccgtgc aaaaatttat
    19561 ccacagaaag gcggcagaaa tccgcaggca atcgggcaat cctcctgcaa agtttctata
    19621 ttgattgaca aaagcggcaa attggagtgt aattcacggt ttaattatct acccattcta
    19681 ttttaggaac catcatgaaa cgcacttatc aaccttccgt taccaaacgc aaacgcaccc
    19741 acggcttcct cgtgcgctcc aaaacacgcg gcggacgcgc agtattggcc gcacgccgtg
    19801 ccaaaggccg caaacgcctg gcagtgtaat tttggactac cgcttcggaa ggcagtaccg
    19861 cttgttaaaa acggatgatt tttcatccgt ttttgcattc agaaaccgcc gcagccgcga
    19921 cctgctgcaa gtttcgcgct caaacggcaa cgggctggac catccccgca tcggtctggt
    19981 ggtcggcaaa aaaaccgcca aacgcgccaa cgagcggaac tatatgaaac gcgtcatccg
    20041 cgactggttc agattgaaca aaaaccggct gccgcctcag gatttcgtcg tgcgcgtccg
    20101 ccggaaattc gacagggcta ccgcaaaaca ggcaagggcg gaactggcac aactcatgtt
    20161 cggcaacccc gcaaccggat gcgggaaaca ggtatgatca gaacggtacc ctgcaggcaa
    20221 ggttcagacg gcaacgggtt tcccatacaa ggaacatccc gatgaacttc ctattgtcca
    20281 aactgctgct ggggctgata aggttttacc aatactgcat cagcccgctg attccgccgc
    20341 gctgccgtta tacgccgacc tgttcgcaat acgcggtcga agcggtcaaa aaatacggcg
    20401 cattcaaagg cctccggctc gccatcaagc gcattgcacg ctgccaccct ttcggcggac
    20461 acggacacga ccccgttccc tgacccgacg caatattcaa attgcacgct ttccttttat
    20521 ttcccatcgg tttctatata atgccgtctg aaccttcggg ctggcggcac ggccgtcggg
    20581 tatgaagccc gcccttattc cccgtctatc ggaacacgca acctgcggca tttccgacca
    20641 ttcaggaaac tctatatgga ttttaaaaga ctcacggcgt ttttcgccat cgcgctggtg
    20701 attatgatcg gctgggaaaa aatgttcccc accccgaaac ccgtccccgc gccccaacag
    20761 gcggcacaaa aacaggcagc aaccgcttcc gccgaagccg cgctcgcgcc cgcaacgccg
    20821 attaccgtaa cgaccgacac ggttcaagcc gttattgatg aaaaaagtgg cgacctgcgc
    20881 cggctgaccc tgctcaaata caaagcaacc ggcgacgaaa acaaaccgtt cgtcctgttt
    20941 ggcgacggca aagaatacac ctacgtcgcc caatccgaac ttttggacgc gcagggcaac
    21001 aacattctga aaggcatcgg ctttagcgca ccgaaaaaac agtacaccct caacggcgac
    21061 acagtcgaag tccgcctgag cgcgcccgaa accaacggac tgaaaatcga caaagtctat
    21121 acctttacca aagacagcta tctggtcaac gtccgcttcg acatcgccaa cggcagcggt
    21181 caaaccgcca acctgagcgc ggactaccgc atcgtccgcg accacagcga acccgagggt
    21241 caaggctact ttacccactc ttacgtcggc cctgttgttt atacccctga aggcaacttc
    21301 caaaaagtca gcttctccga cttggacgac gatgcgaaat ccggcaaatc cgaggccgaa
    21361 tacatccgca aaaccccgac cggttggctc ggcatgattg aacaccactt catgtccacc
    21421 tggatcctcc aacctaaagg cggccaaagc gtttgcgccg caggcgactg ccacatcgac
    21481 atcaaacgcc gcagcgacaa gctgtacagc gcaagcgtca gcgtgccttt agccgctatc
    21541 caagccggcg ccaaagccga aactgccgtc aacctgtatg ccggtccgca aaccacatcc
    21601 gttatcgcaa acatcgccga caacctgcaa ctggcaaaag actacggtaa agtacactgg
    21661 ttcgcatcgc cgctcttctg gctcctgaac caactgcaca acattatcgg caactggggc
    21721 tgggcaatcg tcgttttgac catcatcgtc aaagccgtac tgtatccatt gaccaacgcc
    21781 tcctaccgtt cgatggcgaa aatgcgtgcc gccgcaccca aactgcagac catcaaagaa
    21841 aaatacggcg acgaccgtat ggcgcaacag caagcgatga tgcagcttta caaagacgag
    21901 aaaatcaacc cgctgggcgg ctgtctgcct atgctgttgc aaatccccgt cttcatcggc
    21961 ttgtactggg cattgttcgc ctccgtagaa ttgcgccagg caccttggct gggctggatt
    22021 accgacctca gccgcgccga cccctactac atcctgccca tcattatggc ggcaacgatg
    22081 ttcgcccaaa cctatctgaa cccgccgccg accgacccga tgcaggcgaa aatgatgaaa
    22141 atcatgccgt tggttttctc cgtcatgttc ttcttcttcc ctgccggttt ggttctctac
    22201 tgggtggtca acaacctcct gaccatcgcc cagcagtggc acatcaaccg cagcatcgaa
    22261 aaacaacgcg cccaaggcga agtcgtttcc taaatgccgc agcatgaaaa atgccgtctg
    22321 aaacctgttc agacggcatt tttattgttc accccctatc ggggcggaaa tcttcaaccc
    22381 gcatacatca caaaaatcgt cgggcgtttt ttcagattgg gcatttcctt catcctgcgc
    22441 caatccgcaa ccgttttact gaccaccaac tgagtcggca acgtcaaatc cgtagccgta
    22501 cacaagcgcg tttcaggatg caggttttcc accgcatcgg caagcagcgc atcattgcgg
    22561 taaggcgttt caataaaaat ctgcgtctcg ccgcactgcc gcgaacgttg ctccaaagca
    22621 ttcaaactct gaatgcgctc atttttttca gacggcagat aaccattaaa cgcaaagttc
    22681 tgcccattcg cacccgaagc catcagcgcc agcagcaggc tggacgggcc gaccaaaggc
    22741 cgtacttcaa aaccgtgttt atgcgccaat gccaccaaat tcgcgcccgg atcggcaaca
    22801 gccgggcaac ccgcctcgct gacaatcccc atactgcgcc cttcttgcaa aggtttcagc
    22861 aattccggca aagtcttcaa atccgtgtgt tcattcaacg tttgcagatt cagttcgcgt
    22921 atcggcgtcg ttatccccaa atgcttcaaa tgcgcacgcg ccgttttttc cgcttccacg
    22981 acaaaatccg tcagcccgac aaccgcccgt tgttcatgct gcaacaggca cggcgtgtca
    23041 ggcgtaccca aaggcgtagg aatcaaatac aaaacaggag acatcattcc ctcactcatc
    23101 ggttaaaaat gccgtccgaa cccttcagac ggcataaacg ggcagttaca aaacatccac
    23161 gccctcgttt ttcaaaaaat cgaccagacg gaaaaccggc agaccaatca aggcattcgg
    23221 atcggtactt tcaatccgtt cgatcaacag cgcgcccaag ccctcgcttt tcgccgcaca
    23281 cgaacaataa accgcatccg gctcgcgctc caaatagcgg aggatgtgca gctcgtccaa
    23341 ctgcctcatc acgaccaccg tcttatcgat atgccgatgc atcctgcccg taaccgtatt
    23401 tagcagaacg accgcactgt aaaactcaat ctccctgccg ctcaaatgca tcaacatctt
    23461 ttgcgcgttg gcaaggttca tcggcttgcc ccactgcctg ccgtcgcacc acgccacctg
    23521 gtccgcaccg acaatcaacg ccccggggaa acgcccggtc aacgaccgcg ccttaccctc
    23581 ggcaaggcgc aatgccgtct gaggggcgga ttcccccaac atcggcgttt cgtcaaaatc
    23641 gggggatgcc gcctgaaagg caatgccgag cctttccatc tgttcgcggc ggaaaaccga
    23701 actcgtaccc aaaaccaaag gcagttccaa acccatccca tcctccttac cgttgaaaac
    23761 acgcccgaag gggcggtaaa atccagccat gcgccgaaac acggacgccc gccttcggcg
    23821 taccgcaaca tttttcttaa aaatattgac gttagaacat ctaaattata tcataccccg
    23881 tttatgtcag accctaattt gattgacccg gaaatttttg ccgccgaaag gcagaacctg
    23941 caaggcagtt ttctgctgga agaattggac gagcgagtca gtttgcacga ttatcccgcc
    24001 gacaggcgga ccaaaatatc gtttacactg accggcggtc gcgaccggct gcaacgcctg
    24061 ttcctcgacc tgaacgtcaa agccgatatg cccctgattt gccagagatg tatcaaaccc
    24121 atgccgttca tgctcgatga aagcagccgt atcgtcctgt tttccgacga agagtccttg
    24181 gacgaatcca tgcttgccga cgaagaactc gaaggcatac tgattgaaaa agaactcgac
    24241 gtgcgcgcat tggtagaaga ccaaatcctg atgtccctgc ccttttcgcc gcgacacgga
    24301 cactgcggca atacccttcc ggaatccgcc aaccaagaca aacccaaccc ctttgctgtt
    24361 ttggcgggtt tgaaaagcag ttaattagga cacagtttat ttatctagga gcttgaaatg
    24421 gccgttcaac aaaacaaaaa atccccttcc aaacgcggta tgcaccgttc gcacgacgca
    24481 ctgaccgcgc ccgcactgtt tgtcgacagc acaaccggcg aagtacaccg cccgcaccac
    24541 atctccccca acggtatgta ccgcggccgc aaagtggtca aagccaaagg cgaataatcc
    24601 ctattcgact gactgaaaaa gccagaacat tgccatgcaa ttactggctt tttttacatt
    24661 ggacgcacca tccgtccaaa ctttcgccat acgtcaacac acaggggcaa agcgttccgt
    24721 ataatacccc gtgaaaatat tccaaaagcc ccaaccacca aggaaattcc gatgaaacag
    24781 aaaatctggt acacctacga tgacatccac cgcgtcatca aagcattggc agaaaaaatc
    24841 cggaacgccg gcgtcaaata cgatgccatg atcgctatcg gcggcggcgg ctttattccg
    24901 gcacgcatgc tgcgctgttt tctggaaatt ccgatttatg ccgtaaccac cgcctattac
    24961 gacagcgaca gcgaaggaca agttaccgaa gaagtcaaaa aagtccaatg gctagacccc
    25021 gtccccgaag tcctgcgggg caaaaacgtg ctcgtcgtcg atgaagtgga cgacagccgc
    25081 gtaaccatgg agttctgcct gaaagaactg ctcaaggaag acttcgacac tgtcggcgtc
    25141 gccgtcctgc acgaaaaaat caaagccaaa gcaggcaaaa tccccgaagg cattccctat
    25201 ttcagcggca ttaccgtaga agactggtgg atcaactatc cgtgggacgc actcgacatc
    25261 gacgaacaca accgccttgc cgaagccggc cgaggctaac cctttcagac ggcatatttt
    25321 ccgaaccgat gccgtctgaa gcccgcacga cccctgccgc agaccgaaaa tctaccggag
    25381 aaacccctat gattacattg gccgtagatg ccatgggcgg cgaccaagga cttgccgtta
    25441 ccgtacccgg cgcaaccgca ttcctccaag cacaccccga tgtccgcctg attatgaccg
    25501 gcgacgaaac gcagctccgc caagccctga acgcggcagg cgcgccgatg gaacgcatcg
    25561 acatctgcca taccacccag gttgtcggca tggatgaatc ccctcagtcc gccctaaaga
    25621 acaaaaaata ctcctccatg cgcgttgcca tcaaccaagt taaagaaggc aaagcccaag
    25681 ccgccgtatc cgcaggcaac acgggcgcgc tcatggcaac cgcacgtttc gtcctcaaga
    25741 ccatacccgg catcgaacgc cccgccatcg ccaaattcct tccttccgac accgaccacg
    25801 ttaccctcgc cctcgacctc ggcgcaaacg tcgactgcac acccgaacag cttgcccaat
    25861 ttgccgtcat cggcagcgaa ctcgtccacg cgctccatcc tcaaaaagga cagccgcgcg
    25921 tcgggctggt caacgtcggc acagaagaca tcaaaggtac ggataccgtc aaacaaacct
    25981 acaaactgct gcaaaacagc aaactcaact ttatcggcaa catcgaaagc aacggcatcc
    26041 tgtacggcga agcagatgtc gtcgtcgccg acggctttgt cggcaacgtc atgctcaaaa
    26101 ccatcgaagg cgcagtcaaa ttcatgagcg gagccatccg ccgcgaattc cagagcaacc
    26161 tgttcaacaa acttgccgcc gttgccgccc tacccgccct caaaggcttg aaaaacaaac
    26221 tcgatccgcg caaattcaac ggggccatcc tgctcgggct gcgcggcatc gtcatcaaaa
    26281 gccacggcgg cacagacaaa accggtttcc gctacgcact cgaagaagcc taccacgaag
    26341 ccaagtccgc cggcctttcc aaaatcgaac aaggcgttgc cgaacaactc gccgcactgg
    26401 aagccgccca aaacgaaacc gccgccagtc tgtaacacgc acgatgccgt ctgaacgccc
    26461 ccgccccttt cagacggcat ccggcacccg tccaaacctg cgggcgcgga cggcgatgcg
    26521 cctgtccggc acttcccaaa tacttccctg taaaataagg agtatttgaa aaatgaagac
    26581 attagaaaaa cggatgaaag ctctagacaa acggatgatg aagttcggaa aatcccttga
    26641 aggcaggctt gatgcccgtc tgattgaatc cgcattggat tatattcatt attcggaacg
    26701 ttttttggct tttgacatcc tgtgtactta tatcgaagat ttcgatatcc ggctgacgga
    26761 acaagaatcc cgggaaattt cttttatcaa caaggaattt gagatagaaa gcacgtccga
    26821 ttaaccaata aagccaatga tttgataaga atgaaaacat cgacggtcgt ttttggcgga
    26881 ttttttatgg cagacaacgg ggagcgaatc caaatccccg ttttggaaaa tcctgacatt
    26941 agggaaatca atcgcttttt ttccgtatca aattttgaga aaaaagccgg cgtccttgtt
    27001 ttcagaatca tccccgagcc ggaatttggc aataccgaat taactgtcta ttttgaaaaa
    27061 ggatattata gtggattaac aaaaaccggt acggcgttgc cccgccttgg ctcaaaggga
    27121 acgattccct aaggtgccca agcaccaagt gaaccggttc cgtactatcc gtactgtccg
    27181 cggcttcgcc gccttgtcct gatttttgtt aatccactat atttgccaat aattcaaaca
    27241 attttagaag gcggggatat taaagtaaaa aaccttaaaa cagaaaatta cagcgggaag
    27301 gcaagggaaa tcttgggaga ttgctaccct attgaacata tctctaaaaa tatttctaca
    27361 attcaaaata tcatttccga atttatcaga caaaaacaaa caccggcacc aaataatttg
    27421 atggaaaccc aacaatcaag atgccgtctg aagcagcttg ggctttcaga cggcatttcc
    27481 ttcgcttaaa acagcgtatc ggcaaccccg ccccgcccgt ccacggcaat ccgcatctga
    27541 aaaccatctg tatgccaaac cacaccccca tccctgtttc cgtcatttgc accctgcccg
    27601 tattgggcaa tcatcggttt ttcgcttaca atagctgaat ctgaaccaac tctctaaaaa
    27661 ggccgttccc atgcagtatg caaaaatttc cggcacgggc agctatcttc ctgccaaccg
    27721 cgtcagcaat gacgaccttg cccaaaaggt agatacctct gacgagtgga ttaccgcgcg
    27781 tacgggcatc aaattccgcc atattgcagc cgaaaacgaa aaaaccagcg atcttgcagc
    27841 cgaagcggcg cggcgcgctt tggcagatgc caaattaaat ataaacgaca ttgatttgat
    27901 tatcgtggcg acggcaacgc cggatatgca gtttccttct accgccacca tcgtgcagca
    27961 aaaattgggc atcaccaacg gctgccccgc gtttgacgtg caggcggtgt gcgcaggctt
    28021 tatgtacgcg ctgactacgg caaatgccta cattaaaagc ggtatggcga aaaacgcgct
    28081 ggtcatcggc gcggaaacct tcagccgcat cgtcgattgg aacgaccgca caacctgcgt
    28141 gctgttcggc gacggcgcgg gcgcggtggt tttaagcgcg tccgacaaac cgggcatcat
    28201 ccacagcaaa ctcaaagccg acggcaatta tctgaaactc ttaaacgtcc ccgggcaaat
    28261 cgcctgcggc aaagtttccg gttcgccgta catttcgatg gacggtcccg gcgtgttcaa
    28321 gtttgccgtc aaaatgctgt ccaaaatcgc cgatgacgtt atcgaagaag caggttacac
    28381 cgccgaacaa atcgactgga ttgttcccca tcaggcaaac cgccgcatta tcgaatcgac
    28441 cgcgaaacat ttgggtttga gtatggacaa agtcgtcctg accgtccaag accacggcaa
    28501 cacgtccgcc gcatccattc cgctggcttt ggacgcgggc atccgcagcg gacaaatcaa
    28561 acgcggtcaa aacctgctgc tcgaaggcat aggcggcggt ttcgcgtggg gcgcggtgct
    28621 gttgcaatat tgaaaccggt gccgtctgaa acaggctttc agacggcatt tcccatatca
    28681 tgaagcggca ggctttcttc aaaccgatgg cgtgtgcggc atttctgtcc gccgtttcgc
    28741 tgcgcctccc cgtattgggc gcgtgttacg caatattgtc cctctatgcg tttgcacttt
    28801 acggcatcga caaacggcgt gccgtgcggg gaaaacgccg cattcccgaa caccgcctgc
    28861 tcctgcctgc cttgttcggc ggttggacgg gcgcatactt gggtagtagg atgttcaggc
    28921 ataaaacggc gaaaaagcgt tttgttgtgc tgttccgtct gactgtttcg ggcaatgtcc
    28981 tggcgacctg catcctgatt gattatttcg ttccgcccga actttttgta aaactcgggc
    29041 aacatctctg acaatccaac ccaacaaagg aatactatgt ctttcgcatt cttcttcccc
    29101 ggacaaggtt cccaaagcct cggcatgatg aacggctttg ccgaacacgc catcgtcaaa
    29161 aacacatttg acgaagcctc cgccatattg gggcaggact tgtgggcgat gattaacggc
    29221 agcgatgccg aaatcatcgg tcaaacagtc aacacccagc ccattatgct cgccgccggc
    29281 gttgccgttt accgcgccta tttggaagtg ggcggcaaaa cgcctgccgc cgttgccgga
    29341 cacagcctcg gcgaatacac cgcgcttgtc gccgccgaag cattggattt tgccgatgcc
    29401 gtcaaactcg tccgcctgcg cgccgaactg atgcagtccg ccgtaccgca aggcgtaggc
    29461 gcaatggcgg ccattttggg tttggaagat gaacaggtca ggcagatttg tgccgaatcc
    29521 gcacagggcg aagtggtcga agccgtcaac ttcaactcgc ccggacaagt cgtgattgca
    29581 ggcaacgccg ccgccgtcgg acgcaccatg gctgccgcca aagaagccgg agccaaacgc
    29641 gccctgccgc tgccggtatc cgttccttcc cattgcagcc tgatgaaacc cgccgccgac
    29701 aaacttgccg aagcactcaa aaccgttgaa atcaagcagc cgcaaatccg tgtcatccac
    29761 aacgccgacg ttgccgccta cgatgatgcc ggcaaaatca aagacgcgct cgtccgccag
    29821 ctttacagcc ccgtgcgctg gacggaaacc gtcaacgccc ttgtttcaga cggcattgcc
    29881 gaatccgccg aatgcggtcc gggcaaagtg ttggcgggct tggcaaaacg catcaacaag
    29941 gccgccgctt gcagcgcact gaccgatgcc ggacagatta ccgcctttat cgaagcgcac
    30001 tgacttcgtt ccgcaaaaag cagcctgccc tcttcaggct gcttttcacg tccgaacggc
    30061 aaacagccgc atatttgcgc tataattcat ccctaccaaa ccaccgacag cggctgccgt
    30121 tgcagttccc gccctaccga tatgatagaa aaactgactt tcggactgtt taaaaaagaa
    30181 gacgcgcgca gctttatgcg cctgatggcg tacgtccgcc cctacaaaat ccgcatcgtt
    30241 gccgccctga ttgccatttt cggcgttgcc gccaccgaaa gctacctcgc cgccttcatc
    30301 gcccccctga tcaaccacgg cttttccgca cccgccgcgc cgcccgagct gtccgccgcc
    30361 ggcatcattt ccaccctgca aaactggcgc gaacagttta cctatatggt ttgggggacg
    30421 gaaaacaaaa tctggaccgt ccccctcttc ctcatcatcc tcgtcgtcat ccgtggcatc
    30481 tgccgcttta ccagcaccta tctgatgact tgggtctccg tgatgaccat cagcaaaatc
    30541 cgcaaagata tgtttgccaa aatgctgacc ctttcctccc gctaccatca ggaaacgccg
    30601 tccggcaccg tactgatgaa tatgctcaac ctgaccgaac agtcggtcag caacgccagc
    30661 gacatcttca ccgtcctcac gcgcgacacg atgatcgtta ccggcctgac catcgtcctg
    30721 ctttacctca actggcagct cagcctcatc gtcgtcctga tgttccccct gctctccctg
    30781 ctctcgcgct actaccgcga ccgtctgaaa cacgtcattt ccgactcgca aaaaagcata
    30841 ggcacgatga acaacgtgat tgccgaaacc catcagggac accgcgtcgt caagctgttc
    30901 aacgggcagg cgcaggcggc aaaccggttc gacgcggtca accgcaccat cgtccgcctc
    30961 agcaaaaaaa tcacgcaggc aacggcggca cattccccgt tcagcgaact gattgcctcg
    31021 atcgccctcg ccgtcgtcat cttcatcgcc ctgtggcaaa gccaaaacgg ctacaccacc
    31081 atcggcgaat ttatggcatt catcgtcgcg atgctgcaaa tgtacgcccc catcaaaagc
    31141 cttgccaaca tcagcatccc tatgcagacg atgttcctcg ccgccgacgg cgtatgtgca
    31201 tttctcgaca ccccgcccga acaggacaaa ggcacgctcg cgccgcagcg tgtcaaaggg
    31261 cgcatcagct tccgcaacgt cgatgtcgaa taccgttcag acggcatcaa agccctcgac
    31321 aacttcaacc tcgacatcag acaaggcgaa cgcgtcgccc tggtcggacg ttccggcagt
    31381 ggcaaatcca ccgtcgtcaa ccttctgccc cgctttgtcg aaccgtctgc cggcaacatc
    31441 tgcatagacg gtatcgacat cgccgacatc aaactcgact gcctgcgcgc ccaattcgcc
    31501 ctcgtctccc aagacgtatt cctgtttgac gacaccctgt ttgaaaacgt ccgatacagc
    31561 cgtcccgacg ctggcgaatc cgaagtcctg tccgccctcc aagccgccaa cctgcaaagc
    31621 ctgatcgacg catccccgct cggactgcac cagcccatcg gatcgaacgg cagcaactta
    31681 tccggcggac agcggcaacg cgtcgccatt gcccgcgcca ttttgaaaga cgcgccgata
    31741 ttattattgg acgaagccac cagcgcattg gacaacgaat ccgaacgcct cgtccaacaa
    31801 gcgctcgaac gcctgatgga aaaccgcacc ggcatcatcg tcgcccaccg cctgaccacc
    31861 gtcgaatccg ccgaccgcat catcgtcatg gatggcggca aaatcatcga acaaggcaca
    31921 cacgatcaac tcatgttcca aaacggctac tacaccatgc tgaggaacat ctccggaaaa
    31981 gataccgccg ccgttcagac ggcataaaca aaatgccgtc cgaaatggta taatcgcccc
    32041 gaccccttca gacggcacta tatccgccga cccatccgat tatcttcaac caccgtaaaa
    32101 cccattatga cccaagacaa aatcctcatc ctcgacttcg gttcgcaagt tacccggctg
    32161 atcgcccgcc gcgtgcgcga agcacacgtt tactgcgagc tgcattcctt cgatatgcct
    32221 ttggacgaaa tcaaagcctt caaccccaaa ggcatcatcc tttccggcgg ccctaattct
    32281 gtttacgaat ccgactatca agccgatacc ggtatttttg atttgggcat tccggttttg
    32341 ggcatctgct acggcatgca gtttatggcg caccacttgg gtggcgaagt gcagcccggc
    32401 aaccagcgcg aattcggtta cgcgcaagtc aaaaccatcg acagcggact gacacgcggc
    32461 attcaagacg acgcgcccaa cacactcgac gtatggatga gccacggcga caaagtgtcc
    32521 aaactgcccg acggtttcgc cgtcatcggc gacactccgt cctgcccgat tgccatgatg
    32581 gaaaacaccg aaaaacaatt ctatggcatc cagttccacc ccgaagttac ccacaccaaa
    32641 caaggccgcg ccctgttgaa ccgctttgtc ttggatattt gcggcgcgca accgggctgg
    32701 acgatgccca actacatcga agaagccgtt gccaaaatcc gcgaacaagt cggcagcgac
    32761 gaagtgattt taggtctgtc cggcggcgtg gactcttccg tagccgccgc gctgattcac
    32821 cgcgccatcg gcgaccaact gacctgcgtg ttcgtcgatc acggtttgtt gcgcctgaac
    32881 gaaggcaaaa tggtgatgga tatgttcgcc cgcaacttgg gtgtgaaagt gatacacgtc
    32941 gatgccgaag ggcagtttat ggcgaaactc gccggcgtga ccgaccctga gaaaaaacgc
    33001 aaaatcatcg gcgcggaatt tatcgaagta tttgatgccg aagagaaaaa actcaccaac
    33061 gccaaatggc tggcgcaagg cacgatttac cccgacgtaa tcgaatccgc cggtgcgaaa
    33121 accaaaaaag cccacgccat caaatcccac cacaacgtcg gcggcctgcc tgaaaatatg
    33181 aagctcaaac tgcttgagcc cttgcgcgac ttgttcaaag acgaagtgcg cgagttgggc
    33241 gtggctttgg gcctgccgcg cgaaatggtg taccgccacc ccttcccggg ccccggtttg
    33301 ggtgtgcgca tcttgggcga agtgaaaaaa gaatacgccg acttgctgcg tcaggcggac
    33361 gatattttca tccaagaatt acgcaatact accgacgaaa acggcacgtc ttggtatgac
    33421 ctgaccagcc aggcatttgc cgtgttcctg cccgtcaaat ccgtcggcgt gatgggcgac
    33481 ggccgcactt actactacgt cgtcgcactg cgcgcagtca tcaccagcga ctttatgact
    33541 gcacactggg cagagctgcc atactcactg ctcggccgcg tgtccaaccg catcatcaac
    33601 gaagtcaaag gcatcaaccg cgtggtgtac gatgtcagcg gcaaaccgcc cgccaccatc
    33661 gagtgggaat aaacagcaaa cagggctgca ccgtccggcg cagtccttcg attatcggaa
    33721 aaaaggaaaa aatatgagca cacaagattt aagcggcaaa atcgctttgg taaccggcgc
    33781 atcgcgcggc atcggcgcgg caattgccga cacactggcg gcagccggtg ccaaaatcat
    33841 cggtacggca accggcgaga gcggtgcggc ggcgattagc aaacgtctgg cacaatgggg
    33901 cggcgaaggc cgcgtattaa attccgccga acccgaaacc gtcgaaaacc tgattgccga
    33961 catcgaaaaa acgttcggca aactcgacat tctggtcaac aacgccggca tcacccgcga
    34021 caacctgttg atgcgtatga aagaagaaga gtgggacaac atcatgcagg tcaacctcaa
    34081 atccgtgttc cgcgcttcta aagccgtctt gcgcggtatg atgaaacaac gttccggccg
    34141 catcatcaac atcacctccg tcgtcggcgt gatgggcaat gccgggcaaa ccaattatgc
    34201 cgccgcaaaa gcgggcttga tcggttttgc caaatctatg gcgcgcgaag tcggcagccg
    34261 gggcattacc gtcaactgcg tcgccccggg ctttatcgat accgatatga cccgcgccct
    34321 gcccgaagaa acccgccaaa catttaccgc ccaaaccgcc ttgggcagat tcggcgacgc
    34381 gcaagacatc gccgatgcgg ttctgttcct cgcttccgat caagcgaaat acatcaccgg
    34441 tcaaacgctg cacgtcaacg gcggtatgct gatgccctaa cggacaggct tttcaaccat
    34501 gccgtctgaa gccctttcag acggcatttg cattcccaaa caaaatgaac actcaccacc
    34561 acctgccctg cccctgcggt tcaggcacaa gctatgccgg ctgctgccga ccgcttcatc
    34621 tgcgccaaat cctgccgccc accgccgaag ccctcatgcg ttcgcggtac ggcgcgtatg
    34681 tgctgcacct tatcgactac atcatcgcta ctaccgttcc ggcgcaacaa acattcctcg
    34741 atgccgccga actgatgcag tggagcagag aagccgaatg gctgggcttg aatgtcatcg
    34801 cacaccgcaa cctcggcaaa cagcacgccc aagtcgaatt tgaagcctac ttccaagacg
    34861 gcggaaaccg aacagtgcat cacgagctgt cggcattcgt gaaaatagcg gagcaatggt
    34921 atttcatcga tccggctgtt ccgctgccta ccatgaaaca gccctgcatt tgcggttcgg
    34981 gcaaaaaatt caaagcctgc tgcggcaaat atctgcaacc tgtcgcataa aaatgccgtc
    35041 tgaacgttca gacggcattt tcaacgcgca aaaaaaaaac cattcatacc aagggtaagt
    35101 atgaatggtc aatacattgc gggaaaacat cttacttgct gcactgccga aaagggagaa
    35161 acggcagtgt taaccagcaa aagcattgta cctttattaa tgttaaaaaa cattaatttt
    35221 gaaaatatat taacgaatat atctggaacc tactggtttt cccgtatcca cccgacccag
    35281 cgttcaaaca gcttcggttc gagcgcggcg acgaccgagc gtttgaacac gtgttcacca
    35341 ctccaaaacc cgtcgccttc caaagtcgtc agcctgccgc ccgcctcctc gaaaatcaac
    35401 gcacccgcag cataatccca cagcttctgc ccgccgtgga cataaacatc ataacgcccg
    35461 cacgccagat aacaccagtc cagcgtgctg ctgcccatac tgcgtatcgt cccgaaaggc
    35521 gcgagcgtac tcatgcggct ggaaagtttg cccgaacgca gatatttgat ttccacgccc
    35581 gcaatcgcct cattgagttt tttatccacg agacgcaaag gcaggcgtgt cccattaaga
    35641 aacgcaccct gcccgcgttc ggcataaaaa cattcgccgc tgaccggatt gtagattacg
    35701 cccaattcgg cgcgcccgtt gcggacaaac gccaccgaca cggcaaaatg cggcagcccg
    35761 ttgacaaaat tgttcgtccc gtctatcgga tcgacaatcc acagcccctt ttcccccgaa
    35821 tattgttccc acaaagccga ctgttcctgc cgcgacattt cctcgcccaa catcgggcaa
    35881 tcgatcagaa gcggcaacgc ggcggcaaat gccgtctgcg cggcaatgtc cgcctcgctc
    35941 aacatcgaac cgtcttcctt gcggcgggac ggcgtattca aaaaacgcgg cataatttcg
    36001 gtttgcgcga tatggcgcac gactttctgc aaacggtgta acacttccta ctgtcctcat
    36061 attttgaact tgcggcgcgc gaacgtataa tgtccgtttc catcacgccg ctgcgacgga
    36121 ttataaccgt ccgaaccgcc aaaaactatg cccagattct acctgcccga aaacctttcc
    36181 gtcggacaaa ccgtcgacct tcccgacaac atcgtccgcc acctcaacgt cctgcgcgtc
    36241 cgccccaacg aaaacatcac cctcttcgac ggcaaaggca aggcacatac cgcacggctg
    36301 accgttttgg aaaaacaccg cgccgaagcc gaaatcctgc acgaagacac aaccgacaac
    36361 gagtccccgc tcaacatcac gctcatccaa tccatttcct ccggcgatcg catggatttc
    36421 accctgcaaa aaagcgtcga actcggcgta accgccatac agcccgtcat cagcgaacgc
    36481 tgcatcgtcc gcctcgacgg agaacgcgcc gccaaacgcc tcgcacgctg gcaggaaatc
    36541 gtcatctccg cgtgcgaaca aagcggcaga aacaccgttc cccccgtact gcccatcatc
    36601 ggctaccgtg aagcactcga caaaatgccg tctgaaaaca ccaggctgat tatgagcatc
    36661 aaccgcgccc gcaaactcgg cgacatccgc cacccgtccg gcgcaatcgt ctttatggtc
    36721 gggcccgaag gcggctggac agaacaggaa gaacaacagg catttgaagc cggctttcag
    36781 gcggttacac tcggaaaacg gattttacgc acagaaaccg ccccactcgc cgccatcgcc
    36841 gcgatgcaga cgctttgggg cgattttacc tagcagatga aaaaacacgg gattttatcc
    36901 gaaaaagcaa caatcccccc gccgtcattc ccgcgaaagc gggaatctgg aattttaatg
    36961 cggcaagaat ttatcggaaa aaaccgaagt ttaaagacct agattcccgc ctgcgcggga
    37021 atgacagtgt gtccatttct gattttaatc ccctatattt tacacaaact atttgaacaa
    37081 tatgacccgc ctgccgtaaa ctttctcaag ctccgcctgc ctttgacgct ccattctttt
    37141 cttcttttcc ctaccgaatt tacccaaagc acgcttcaag tcaaacatca ccttcaacga
    37201 acggcggtgt cttcttcctt gttccctatc tttttccaaa tcgctaccca acatactgtt
    37261 ttgactgaga aacttggcat aatgcaattc ttgggtacat aaggcgggac taacctgata
    37321 aacaggcatc ccctccttat caaagaaata agtaaacatc atcaaatcta ccgctttaat
    37381 ccgctctg
//
LOCUS       DAXDXW010000010        37349 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXDXW010000010 DAXDXW010000000
VERSION     DAXDXW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37349
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.010"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..223
                     /locus_tag="ACFB64_000427"
     CDS             <1..223
                     /locus_tag="ACFB64_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208852.1"
                     /translation="TLNLFVEGFWIIGLLQAAVFTFVVDCSSSDLGSLSQWQIGNDWD
                     RQMDPFYAHEIGNIWHGTHLDSSSEEYWK"
     gene            complement(280..438)
                     /locus_tag="ACFB64_000428"
     CDS             complement(280..438)
                     /locus_tag="ACFB64_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208853.1"
                     /translation="MSIAQIVVQIEEVDEGNYQVRMSYSGGNDLVSEEGKSAFEQIIT
                     SIGKVLET"
     gene            complement(442..1023)
                     /locus_tag="ACFB64_000429"
     CDS             complement(442..1023)
                     /locus_tag="ACFB64_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFC1208854.1"
                     /translation="MRNATSYGGNGVMWRGISSGRHVRGNYVLKASTAHNQAELERYV
                     HNPQVRAFLDFIAASEGVKHGYQTAIGNYRITDFSRHPGNSVRRSFTRTDGSRSTSDA
                     SGRYQFMGYTWKGLAKQYGFTDFSPATQDKAAVALILAKKGAMEAILNGDYEQAVMKL
                     GGIWASFPTAPNEYRQHKRSWGFVHNFFRQRGF"
     gene            complement(1029..4031)
                     /locus_tag="ACFB64_000430"
     CDS             complement(1029..4031)
                     /locus_tag="ACFB64_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFC1208855.1"
                     /translation="MMAVEYFTFPGNGEAVRAVFNGVATISGSTMMGGAMQAAALFGF
                     LVTLAVAVFKLDLKDNFSYLFVVLFCWMGMMVPKTTVLITESGGYGYTGRQYTVGNVP
                     VGLAYMGYFVSSFGQSITRKAEQVNHLPDDLNYSRTGMLFGTRLMENIREARIPDAQL
                     TQDWALFMYQCSFFDMNLYHFYNVQDLAQSADILATLTHTNQALFTNVSQIASRRGNM
                     LQYNGKSKTMTCNEAAKELKDRTRFYSKNYLPGYIADRVFAGLGTNSAGINRVNALVT
                     LGNSSFQYLLDNARFDTLKNIEQAAMVEVIRQAGIINGQRNRNPAAVQQAFAQVQARN
                     QYIAAQKTGSSMASWNLPLIRSAAEAILIGLFPFVMILAMLGGIMAFRLLSFYMMSML
                     WIQLWAPVASIINMIMTMNAKRLFSVEAANGVITPGTGDTLLMAAADAQAAAGAAMWL
                     IPVIAGALAMGGRSLMNGMMGMTSSAKSTGEAAGSQVGSGNYSAGNMNYNNSNANKHS
                     LNPVYTDPQMMTAQSAAGTSWRNMATGDSRAQMRNSSFGVSSQSQISQSESYSKAADR
                     AETSAQQWQASFRESVSQGNANRLAFATNYGMDRSASEGYGMGLDAAESRKLSYVIDQ
                     GKKIAQKFGVTNTSAVISAIAAGLGISGNTGSSTKAQNIKNALGAKGDLGWSGKSQNT
                     DQMVAEIGESIEAGKRQGISFDSNFTDKVSQSEAYKVALNSGNSLAQTAEANFSKARE
                     ASHMASESYQKAQSYREMAAQTYGNGITIAQDNTNALVQDNPELTMSELNAGQGGTYN
                     AAIRAGVNSQDRLKAAAENSGWKPNDLSPEDRPQNNTQAAYKHFSDKVSSGAGAAFGD
                     VQTQAHQAGIIPDGISAAINARGENLKQEYANKSDKVEGDINQGRNSHRQEVGRLATH
                     AKNAQDQTNGVTMRINTHALPWNSFGERYDNAMKDVREGKGYIGSGSDQAAAHLGNPA
                     LASEFDLPIPQRPGNSKSSGSAVFRPKE"
     gene            complement(4038..5549)
                     /locus_tag="ACFB64_000431"
     CDS             complement(4038..5549)
                     /locus_tag="ACFB64_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFC1208856.1"
                     /translation="MKFHIFRFPKPAKVPTVCASSAKYLCLAASLALPINSSAGIEKN
                     MADMFNSMGAEANYTEAGAFHGQSGSLYTGGGMSVRFPVSDISLGNIQLPSISAGCGG
                     IDFFAGSFSFVNKEQFIQFTRNLGNNAAGVAFDLALKALDPMIQDAIGGIRDLVNYVN
                     QNSLNSCKLSQDIVGGAMGKLGSLASSNCRAASVDSGSSNDPAEGAWYCQAAKNLVHE
                     ADKVRNTGKPQDTISFTGGNLTYEAMKNYTGQRSKLGIDMDFYYSMLGTIVFTPLTQH
                     EGDKDKYTVGIQSFEPKILSIQDLLNGKNNAGGEIREKVVLDMWTCKDGSKKLMENCT
                     EKNDVEIPSLRYMVHKRIETLPTAIRNNQGWNLSTRKQIAAIVNNSKLPILKMAVSDA
                     FLGTGYLKKTAVLDAIAVDIMANILDQSERQVRSTLGMYNKTDDASGEAVRKLFANLG
                     ELRSRIYQERIDSMKKIEIEESMMRAMEQFETQWRSAFVDTNNSMTFDSMNRF"
     gene            complement(5721..6497)
                     /locus_tag="ACFB64_000432"
     CDS             complement(5721..6497)
                     /locus_tag="ACFB64_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFC1208857.1"
                     /translation="MAVMLIFSLHASNLRAADDGMGWYDQDIWKNENRAFLWYPDAKK
                     PKQAGQNRPQNTPVRNELAEFEMLQRNLDESRKIAVMNPTPQNLKRYIELQEVVMNKS
                     AAFADQWQRVIWQNPNLDYSQRGRPNNQLAQQQYDHNRQQQKAGAIRKLAGENGIMFV
                     FKSDCPYCHTMAPILKNFADQYGIKVMPVSLDGGGINHFPRALPNNGIAQRLNITTVP
                     AMYVMDTKTKQFTPIGFGVMAQTTLEDRFLAYSRPVGTLY"
     gene            complement(6509..6934)
                     /locus_tag="ACFB64_000433"
     CDS             complement(6509..6934)
                     /locus_tag="ACFB64_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208858.1"
                     /translation="MNINNWLAASAGIVSGMFFTGMALAASLPYTGGIALEKTEALAR
                     TSIPLPDGKPDALLALAMRHGSAKGYFSGRAAAAVKQKFRQDIPIFVEAVRMEEIKNK
                     PGCNRVRLTFHTSPALSQTFPSEIMDIGACPNRKNGGKK"
     gene            complement(6921..8462)
                     /gene="traN"
                     /locus_tag="ACFB64_000434"
     CDS             complement(6921..8462)
                     /gene="traN"
                     /locus_tag="ACFB64_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFC1208859.1"
                     /translation="MLITSASAAALRESAACTRTSSVCVDGPSTKNINGVDVTKDCWE
                     YKEEYQCLEKDSADYCAPLKDPSAKCEVQGQTCLEQSNEGECLRYTHKYSCDVDLRTL
                     HQGRLPTKVEEMEHTHLISSQWDESSCQVQGKKCKAVATECLEPGSTKTINGVPVTRD
                     CWKERRTVQCTDGSDSETCSAYTSSDQCRLIGDKCTHQLPDGTCQAREKQFECTEKGE
                     TTKEVSGCQDRDFAKTMTTMEFARETQRFYDPEKQRFFNGEAGQCSIKLDGALDSVFG
                     GDCCRTKADPGKFVDFAVQTGTTMATTYFMASVASHYTFTTMFVSSAAQAMGTALSAA
                     GGITGTSQIGALGFSAAGQQGMGVIVGFNPAVFAAAIAVIAIQQWLKCPQSEILVAMK
                     RKADLCHYVGSYCGSKILGACVTMIESQCCFISKLAKIVNVGGKEQLKRGWGTPENPK
                     CEGFTAQELEQLDFSKLDLSGFYEEIYANMDNVAKQGQKVSQKIREASVNGKNLEVKN
                     YYEYQ"
     gene            complement(8501..9745)
                     /locus_tag="ACFB64_000435"
     CDS             complement(8501..9745)
                     /locus_tag="ACFB64_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208860.1"
                     /translation="MKRHAIYLTVFGLMQTAFAADYGSYVGELGKLGAGTATNMMQPA
                     QYTPRYTDNVPMAEQYYGGGVSLPTQFGDAKFNDCKNLPANPDLYLRQECEGVNLIAG
                     SRTKRPDVTISSNEKLVKGTQKIAGDPAETLEKYKWKYPVNEDGSVGTVPSSACPTET
                     ITVPAVKTVKRCNEYHGAEMFLCEAALKVNVDPGWNYSCLESKYRNQQYQCGKKLKVV
                     CEKSGPNCTRSGIEAGSLQGDMTVRLTASGSAHRLMFGTIGDNYWRDGQYDREMTLNV
                     KNPEKLTLFTLSRVEYDDWLIVKVNGKIVYSSYNNQMFAADYTAKDEDGFPIGRRFAP
                     VRNEEGTRLGNAERGKSWRKNLNIDIRPYLHQGKNTIWTRTVVGGGGENAIFFNVHQY
                     CEPVCHDKWENSCSEYEKRVKQ"
     gene            complement(9742..10494)
                     /gene="trbC"
                     /locus_tag="ACFB64_000436"
     CDS             complement(9742..10494)
                     /gene="trbC"
                     /locus_tag="ACFB64_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFC1208861.1"
                     /translation="MDKSLRNVIVAGGVFLILLGGLAGVGSAYADADVEAARNRLRTT
                     MGKVTRVKNPNVGDNRQQPEIPDLKGIKHRVPDVRDYGNAKIKADPMSIAQKYRTASQ
                     EARQAAEKADLMVFVSFSMPEASLKRIARETAKAGGVMVIRGFKDDSLKATVKAAEEL
                     AALQGDMLIHPELFDHYGITEVPVTVIARNSEDGLDGCAVNSELGMCTEHVAVKGDVS
                     LHSALEHLIRESEDKKLSDIANAKLAVLEGRK"
     gene            complement(10509..11627)
                     /locus_tag="ACFB64_000437"
     CDS             complement(10509..11627)
                     /locus_tag="ACFB64_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HFC1208862.1"
                     /translation="MKIFVTPLKLLIPLLLLPLFSISHAAEAVPTAPIPNKLTCQGKM
                     LNPINDICWSCMFPIKIGGKINLNMDNQEDNGSSPNNWFCSCTHPSRSGVTVSFWEPT
                     HLVEVVRTPFCMVSLGGISLKNQKADTAETTGKADVSNKIQNLFGGSHKRGRNEQHDQ
                     HNAFYQVHWYKNPVVFWLEALLDNDCMEKGTLDIGWMTELDPTWKYPSLAVYQAPDSA
                     LFANVVAQAACAADCVKAAAGFPFNKLYWCAGCHGTIYPLSGWMSGHFSEVQAAKLMA
                     ARIQTKMHRDLVAWRTSGRDALCGYKLNPVMDKQNYKMAMTYPVAARKFRGRCCEPIG
                     RSTLVRDSGKAFPYKGNQFAFQLFRKRDCCGAYKFKTN"
     gene            complement(11624..12280)
                     /locus_tag="ACFB64_000438"
     CDS             complement(11624..12280)
                     /locus_tag="ACFB64_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFC1208863.1"
                     /translation="MSHKKKIASVASLVILATGISSWVAASTPSVEKVGDKIGTTYPI
                     AEKHAIREIEARLKAKQDSGQLAAMQKEVQDRINRSALNLQPLEGLAKATKGSVRYFD
                     PSYTLTETIYDQDGQIIAPAGTVVKPLEAAPIPFKMFFFDGREPDQIELAKKLAAEYG
                     ESFMPILTAGNWYELSVEMDQAVYYDQEGRMSKSFQLNEVPSLVSQEGGRLRVEVMKP
                     "
     gene            complement(12289..12834)
                     /locus_tag="ACFB64_000439"
     CDS             complement(12289..12834)
                     /locus_tag="ACFB64_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HFC1208864.1"
                     /translation="MFEAMIKISQKESNKAVMKRLYREFCQKYATPFLAVAVCLSAFN
                     QHYALAFNLSRSLPHHLYFIKKDANKLSDLKQGDYVAFAWQGGFYPIGTQVVKEVAGL
                     PGNHVTKANRTFFIDGKEVGTAKEYSLDGMKLAINPFEGEIPQRFMWVKTGHKDSLDS
                     RYELSGLIHSGQIIGKAVPIF"
     gene            complement(12834..13382)
                     /locus_tag="ACFB64_000440"
     CDS             complement(12834..13382)
                     /locus_tag="ACFB64_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208865.1"
                     /translation="MTEAKEKKENTANTDPEKNPEDNGVEVSLGSLVIGAISIAALAL
                     GVWNFMKTHEKPVHIVDLAGISMTYREQAKNQGLQNGISDRQRAAILQNYQAKMNTLQ
                     QVIEEKVIRCGCNIFVKSALVGRNTEVVDVTADIMKELDKRVPATAVVAAPVHQQVEK
                     TIPKPMAEEGKGTPVTEEEMDR"
     gene            complement(13410..15986)
                     /gene="traC"
                     /locus_tag="ACFB64_000441"
     CDS             complement(13410..15986)
                     /gene="traC"
                     /locus_tag="ACFB64_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFC1208866.1"
                     /translation="MGVLSKFKNWLAGSDDVDQPMEALHNNPVDPDFDHLSDYLNYGA
                     YDTKTELFTLMSVKGDKPLGMGFILELNPFLGGSDDLIGKWGSLYALLPEKTPIQVQI
                     FGSPDLRNYFKEYEQVQASRPIDDPMRSTFETLAKKRTEFWNKGTQKVLVENTAIRLR
                     NLRCILSVNLNIPPDNAVEVSKAVSLLARMRDSLRSMQIYGRTWNADDLLAWTTLLLN
                     PNRMFTGQQDEIDPVWDETKFLSEQMIETRTSIKVLDSGSGLRFGNRAAGDCVIAQCY
                     SANRYPEEFHLSNMGALIGDVIEANMNYTSPFLISMAMFKRDYDTSSNTVKLKAARAK
                     QTAESKMAAVMPEAAKIKRDYDICLEAFGKGGGGLVSLLHQVVIWERPENINLAESQA
                     VSIWQAQGFGLYRDQYLQLGSYLTALPMAIDKEVEKYLDSKKRWSTKTMTNAVCMSPV
                     IGEWHGLGRPVIGLFGKRGQAMGIDLFANPAGNYNFAIIGASGSGKSFFANEIVRNYM
                     GLGTQVWIIDVGRSYEKFCQMIGGQYIEFTYEAGLCFPPFQMVTDINEDIEMLKLIFS
                     LMASPSDSLTEQQEAKLQRIIEEVWYEFGQEGTVDHVRERCLKCLITGSNEGGKPGER
                     DLSMVAVADQLYTYCSEGIYGSYFNGKLNVEFKNDLVVLELEELKTKPDLQQIIMQLI
                     MYRIMQGMYLSRSQYKIMMIDEAWALLGDTGTAARFIEEGYRRVRKYKGACGTLTQGA
                     NDYYKTKAAEAALENADFVFQLRTGAKSLKTIQDKEPFEVNDGIMQILRTLSKTDYYS
                     EIFVSTPIGYGVGRLYSDPFNVLASSSKAEDVEAVNHYRNQGLNTAEAIEAVLRDRAA
                     TR"
     gene            complement(15996..16577)
                     /locus_tag="ACFB64_000442"
     CDS             complement(15996..16577)
                     /locus_tag="ACFB64_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HFC1208867.1"
                     /translation="MMRLKALSITAAILILSGCSTLTMSGIGGSEKFRCPNARNSDDP
                     YCESISSNYKASVAGVLKDGMKRQVGQPYTDQSVRTLMLTQAYSSGTPVRSQSEIARI
                     WVAPYLDTDGDLNDQSFTYVVLNQGDWLIAHNGQQIINEYRPIRLLGDGAKTSGSSVE
                     TSPTNSNNTNDRIPDVGVNLHLQETSGIPGTVR"
     gene            complement(16574..17293)
                     /locus_tag="ACFB64_000443"
     CDS             complement(16574..17293)
                     /locus_tag="ACFB64_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC1208868.1"
                     /translation="MKKKILATIAAITAFQTAFADVKTVGSTLHKLYPNTTFSSVKAT
                     PMASIYEVTMGDNIAYVQENGRYFIFGALYDMQEQKDLTEMARSAVTQKSYSRLPFKN
                     AIKIVKGNGGKGKREFALFSDPDCPFCRRLEETLAGMTDYTAYVFMFPIKSLHPDAIS
                     KAEHIWCSKDREKAWNNYMLMDKEPAAGNCKNPVSENIALAEQLKVRGTPSMIHKDGR
                     RTSGAMPRAELEKWLNGAGAE"
     gene            complement(17303..18616)
                     /locus_tag="ACFB64_000444"
     CDS             complement(17303..18616)
                     /locus_tag="ACFB64_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFC1208869.1"
                     /translation="MRVKVNKFKLSAARKRQLAIAGMVAGSMAAIVGSAIYVIEGNKK
                     KSAEVTQVQNKRQILRTDFTSPQAGITDNSLWMNTESSKIEYANRKISELETMVQELK
                     EKENSSNPDTSKGLGPDGLGKPPAISGIGDNGRLPPAPPAGTLPNGAPPADRPIERKI
                     VSGSMSEAELQPGTTGSVNTGNPNENVRVSPQLKEAEWVKSKTPRMNIEVVEDGGKVV
                     VSQGKFRARDSYIPSGTFFRSVLLGGVDAPTGGEAQNASPHPVLMRVTDFAQLPNRFK
                     YNFRECFVTGQAYGDISSERAYIRLQNLSCVGTDGRAIDMPVKGYVAGEDGKTGVRGN
                     LVTKQGQLLANALMSGVISGMGKGVSEAFKVTNNTAFGSTTSIRGSDQYRAGIASGIG
                     GAADRLAEYYIKLADKVFPVVEVNAGRQVDVVLTQGIEIDTGETK"
     gene            complement(18618..19352)
                     /locus_tag="ACFB64_000445"
     CDS             complement(18618..19352)
                     /locus_tag="ACFB64_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFC1208870.1"
                     /translation="MCSLLKKIVFSALLAAGPATSFAAQRVPATENTPHVVSISKRNL
                     SRIAIEGGRISSWKFMEGDLELQKDTTTGQLFVRSLTSNPTNLFVISEEGKTYLLVLK
                     PTSKQGDNIVIDVAGANRREAAVLASQTGPMPVTMNSTEYVRAIKKMMASMMRGTTGD
                     MGINHSHEYQTVPLWKNTLFIQNGSYTAADMQGLSFTLTNLGTKQLEIREQEFYRQGV
                     LAVAVRKQILQPGEITDVFIISRPGG"
     gene            complement(19354..20019)
                     /locus_tag="ACFB64_000446"
     CDS             complement(19354..20019)
                     /locus_tag="ACFB64_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HFC1208871.1"
                     /translation="MKTEDMLSELAIRTGIKVFFWILLGMSIAVNLFLSATLLFKGTI
                     VQTTLTPPEIRRSMTVSNIAFSKEYLEEMAPYAAYLLLNVTPKTVDYQNSQLLKIVSP
                     DYKDALERELSLNALWVKKNNVGTTFSATEATADISDNTVSIRGVFEVKRNNALVEQK
                     DRELLISFKNNYGTLQLLSIKEVQKRVTKAEPAGQEKSEVETEDVEISKTATEFKGGN
                     RGN"
     gene            complement(20016..20297)
                     /gene="traL"
                     /locus_tag="ACFB64_000447"
     CDS             complement(20016..20297)
                     /gene="traL"
                     /locus_tag="ACFB64_000447"
                     /inference="COORDINATES: protein motif:HMM:TIGR02762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFC1208872.1"
                     /translation="MRDYHVPVSIDDPKKAFIWDLDVFILFMSGFGIGIVSRHFFISM
                     SISLFIAWRWGKLKSGKHQWFFLHVLYWYLPINEKNKRIPETNKREFLN"
     gene            complement(20319..20615)
                     /gene="traA"
                     /locus_tag="ACFB64_000448"
     CDS             complement(20319..20615)
                     /gene="traA"
                     /locus_tag="ACFB64_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraA family conjugative transfer protein"
                     /protein_id="HFC1208873.1"
                     /translation="MNIKRNKLVKSASAVALALYANLAMAATTGAEFKGLADMVTGWS
                     EGYLGMALGLTAFLIGLAVGLMKQTIMPCIVGLGVGLAATLGPGIIKGMFTLII"
     gene            complement(20671..21192)
                     /locus_tag="ACFB64_000449"
     CDS             complement(20671..21192)
                     /locus_tag="ACFB64_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFC1208874.1"
                     /translation="METIKNTLFVASAMILTACAAPKQEVAGLPQDATYTVEQNQLGE
                     FVFCRNEECSGFERKVLAEQPVYAQTQTNLQKDEIRVFFNLGSSHLGAEGVRTLKRVL
                     PKLKNTQTVYLRGWADSIGGRNTAINRRLAKQRAAVIGKWLRKHNVSAKIRTASSPAC
                     CNSDDTRTVVITW"
     gene            complement(21162..21623)
                     /locus_tag="ACFB64_000450"
     CDS             complement(21162..21623)
                     /locus_tag="ACFB64_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC1208875.1"
                     /translation="MRLRAILLQFVVPGVLVLTSARAVAFCYKEAGSKYGIDPAMLQS
                     IALTESAFRPNIESHTADIGLMGINRSWLPVLHKKFGLTGADVWNPCTNVHIGAWILA
                     NSYRQHGKSWEAVGAYNAACTKLKGRACYRARMTYANKVYQNWKRLKTRSS"
     gene            22134..22553
                     /locus_tag="ACFB64_000451"
     CDS             22134..22553
                     /locus_tag="ACFB64_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208876.1"
                     /translation="MNETDLPKLLSVINRFTDIDKNWSCVLLFWIQRASGYLEMMTLD
                     DDENTATFLIEKLEKLLEPLPIDIDNTTFAEALADDFEILFLMAQYGSEYWNSLEESF
                     EEFCIRHTTLPNQLIADIPHAKKEKVTMWLKSLLKSS"
     gene            22550..25102
                     /gene="mobH"
                     /locus_tag="ACFB64_000452"
     CDS             22550..25102
                     /gene="mobH"
                     /locus_tag="ACFB64_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HFC1208877.1"
                     /translation="MKTSLLTIASSLMLVSGSMLYLINTRKSTDSVSHNDEVAHINQN
                     IVNDRFRILNAHELIQVLDLSPQISGIKMNLGLSDENWSKDALPFLEKYIAFVQRLPA
                     SESHHHAGDGGLVRHTLDVAALALVASTSQSWPPNAKTEEIAKKTAVWRYGIMCAALL
                     HDVGKTVTGFQVELFDSAISLEKLLWLPDTGSMAESGKLYYRVEFPDAKSAYSTHAEI
                     AWTFFQALVPSHVRQWLATTDPNLMITLRNYLSGKKDGSPLEQLIKNADMTSVSRDLR
                     SGSRQRFSTAKRKPFIETIMETLKEMLSDRGVHFSIATTAGGDLFRKGDRIYLMSKNV
                     PDYIRQYLRKNQHPAAGSFPADNQRIFDTLFEYRAVIPPENDPHRAINHIEVEFTRMD
                     GEKVRNIFSVLCFNAKTLYPDGDYPTEFLGNLSEVSAKKEIPDVVVSEDKVSTQIQGA
                     EDNPVTMTKDKMDFLSIQPVTAEQPPLPEKNQTETANSAFDNSMNTAAPAENKSSESE
                     KAKEPGYGTIDNLIENFNLIEADSEQTESTKSEAETAENAVNLVQENIRVEVKTETVD
                     KPRAEVKETETDKAPKTIKSGTKASRKKLAGLFADNRKPIGKTAEPDAQIKTTETVTH
                     QIDSEAVSLPESESAQNGMRELEQLRLREPTSSPRPVQVMDDSEGLDSLASGVKNMAE
                     LEAIVSERNLKKQSETESTEIVEDSVKAKHMEARDRGMQFLRWLADGLGDGSIAVNRS
                     GATVHFIEQGMLLVTPAVFRDYAGGVFNKSDTESLGPLTQKGFEALRLHARTKKSSLH
                     RVLTTNGSNRRLFYCYLIPEHNIKHIIQPGSRPQNNTDIKLDESDLLVMEQK"
     gene            25099..27141
                     /gene="traD"
                     /locus_tag="ACFB64_000453"
     CDS             25099..27141
                     /gene="traD"
                     /locus_tag="ACFB64_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFC1208878.1"
                     /translation="MSAHFPENVYRSPYEWISVAAYSTTAVSVAHLPVPPTIAYGSLA
                     IFSLVSVKRALSALRIMRFRKNIRKLPYYALTADEIPSSNKALFLGKGFRWTQTHTQR
                     LFMVRQPENDGLRNPSKLYKAARKHEIETMGTDWISRITRKSAWWNPVAPLPPVGGSP
                     ELHGIEIEEDDIWEDLGERVGHKVVLGTTRVGKTRLCETMIVQDIRRGDVTIAFDPKG
                     DADLMTRMYVESQKSNRPFYMFHLGFPEYSCRYNPIGDYGRITEVATRIANQLPSEGQ
                     SAAFRDFVWRFVNVLTKTMEGLSIKPSYENIYKSAANVDELAGDYFKKILDKHHPGWE
                     KDFDNFDMPESEAKSAVKTGRNLDTMKLGSFLKAKKHNEPLIDALGGILSNDRSYFEK
                     LVSSLYPLLEKLTTGEVAKLISPDTEDLSDPRIIMDWRKVMESNAVVYIGLDSLSDAE
                     VAAAVGNAMFADLTSLAGQIYKFGHGVGQSGKTQKRKVSIHADEFNELIGDEFIPLLN
                     KAGGAGYQVTVYTQTWKDVEAKIGSPAKAGQIGGNLNTMIMLRVKTVETAEMLTNQLP
                     EVKIMTSTLVSRAGDVAFPDSHEDFSSGNEDRIGAETVPMLTPADLTQLPKGQAFALI
                     EGGQLYKIRLPLPKKDKQEEIPAHIGEMAADMREVYRLSRPSAEENAFTEGSSYAV"
     gene            27131..27826
                     /locus_tag="ACFB64_000454"
     CDS             27131..27826
                     /locus_tag="ACFB64_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HFC1208879.1"
                     /translation="MPSNTSAWYMNSLPLKFLFLPFKAAYTLFLIVLLIFGSSVLTQN
                     YFGTGNLLEDEIKWSESVGHDAFPGKEVDDTINLQIARLSYKGLKTVFFDLTLVNESL
                     KAKSEADFGYKFKNYLVPRMKYLQQFDTTLKVISLRLGNLALIVGISVIFAIPVLIDG
                     LVMRAIRQENASRESAGIYHRAKYWRTGIIWLGCMIYMCVPISIPAYLLYLPLVAAIW
                     MIFMQAKYLKKYL"
     gene            complement(28271..28783)
                     /locus_tag="ACFB64_000455"
     CDS             complement(28271..28783)
                     /locus_tag="ACFB64_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208880.1"
                     /translation="MKSKLPLILINLSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYILPQTPEPAPSPSNGGQAVKYKAPVKTVSKPAKSNTPPQQA
                     PVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQKINALQSNVLDRQQNI
                     QALQRELGRM"
     gene            complement(28874..29269)
                     /locus_tag="ACFB64_000456"
     CDS             complement(28874..29269)
                     /locus_tag="ACFB64_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC1208881.1"
                     /translation="MPSDKEARAGCLSLPANNGIIRTLPPPFYFTGINIRLFAALGLL
                     SLSGAAAQASIYHCNSNGKSVYTSDPSGSCADADLPKISSHQGGGYRLKIKKLSQEAK
                     IHTGNKKKNKKHAGKKNRQAAKAPKENQK"
     gene            complement(29256..31178)
                     /locus_tag="ACFB64_000457"
     CDS             complement(29256..31178)
                     /locus_tag="ACFB64_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC1208882.1"
                     /translation="MIEIKNLTLQRGLKVLLDKASAAVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDISIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     VQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYSGNYDFYQTERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYNPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVHSEKLNIGYFAQHQLDTIRADQ
                     SPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIVVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAATVPAASTQSQNRKDTKRIEAQIRQEKARRSKPIQQKIDKA
                     EKEMVQLSEIQTGCEAFLTQEDAYLEANKEKLQNTLSELAKVKTQLAQIEETWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(31255..31626)
                     /locus_tag="ACFB64_000458"
     CDS             complement(31255..31626)
                     /locus_tag="ACFB64_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC1208883.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVHKDSVCLPQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            31822..33129
                     /locus_tag="ACFB64_000459"
     CDS             31822..33129
                     /locus_tag="ACFB64_000459"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFC1208884.1"
                     /translation="MKPVNIGLLGLGTVGGGAAAVLRDNAEEISRRLGREIRISAMCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKEAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     DGVMNAVRVNANMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            33122..33556
                     /locus_tag="ACFB64_000460"
     CDS             33122..33556
                     /locus_tag="ACFB64_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208885.1"
                     /translation="MTEPKHETPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCMKNIPFAEKWQNDLKARGLDADNTRLAVDYCKCMWEQPLD
                     GLSEKQISSFGKLGAQEQLDLLGGANAFETRDKQCVADLKAD"
     gene            complement(33732..34001)
                     /locus_tag="ACFB64_000461"
     CDS             complement(33732..34001)
                     /locus_tag="ACFB64_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC1208886.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTNALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTLKFRAGKALKDAL"
     gene            complement(34183..36645)
                     /gene="lon"
                     /locus_tag="ACFB64_000462"
     CDS             complement(34183..36645)
                     /gene="lon"
                     /locus_tag="ACFB64_000462"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC1208887.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAKLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLGLPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVRNPLFLLDEIDKLGSDFRGDPASVLLEVLDHE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELVVEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLNE
                     DKKRLSETKKTSKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGMIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESRPESWAEP
                     SAAEAAAESASKPKPRSRAIKH"
     gene            36897..>37349
                     /locus_tag="ACFB64_000463"
     CDS             36897..>37349
                     /locus_tag="ACFB64_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC1208888.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWYYVRESGADGILKATYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGVHEWHK"
BASE COUNT         9467 a         8947 c         8405 g        10530 t
ORIGIN      
        1 gacattaaat ctttttgttg agggtttttg gataattggc cttctccaag cagcagtatt
       61 tacgtttgtg gtagactgct caagtagtga tttaggttca ttaagtcaat ggcaaattgg
      121 aaatgactgg gacagacaaa tggatccatt ttacgctcat gaaattggta acatttggca
      181 tggaacgcac ttagactcga gttctgagga gtattggaaa taaaaaacag gagatttata
      241 aactcctgtt tttttatttc caaaagatgg ttaaattatc tatgtttcta gtaccttacc
      301 aattgaagtg atgatttgtt cgaaagctga tttgccttct tcggatacta gatcgttacc
      361 tccactataa ctcattctaa cttgataatt cccttcatca acctcttcaa tctgaacaac
      421 aatttgtgca atactcatct tttaaaatcc tctctgccta aagaaattat gtacaaaacc
      481 ccaacttcgt ttgtgttgcc ggtattcatt aggagctgtt gggaagctgg cccagatacc
      541 acctaatttc atgactgcct gctcgtaatc gccatttaaa atagcttcca ttgcaccttt
      601 tttagctaaa attaaggcga cagcagcttt gtcttgtgtg gcgggactaa aatccgtgaa
      661 gccatattgt tttgccagtc ctttccatgt gtatcccatg aattgatagc ggccggaggc
      721 atcagatgtg cttctactac catcggttcg agtaaaacta cgtctaacag agttacccgg
      781 gtgacgggag aagtccgtaa ttctataatt acctattgcc gtctgataac catgttttac
      841 accttctgat gccgcaataa aatctaaaaa ggcgcgcact tgcggattat gaacatatcg
      901 ctccaattcg gcttgattat gagcagttga agcttttaat acatagtttc ctctcacatg
      961 tcttccactt gatattccac gccacataac cccgttgcct ccatatgagg tggcattacg
     1021 caacggggtt attcctttgg gcggaataca gcggatccgc tagacttcga attccctgga
     1081 cgttgaggaa ttggtaggtc aaattcggat gctaaagccg ggttgcccaa atgggctgct
     1141 gcttgatccg acccagaacc tatgtatcct tttccttcac gaacgtcttt cattgcatta
     1201 tcgtatcttt caccgaaaga gttccagggt aaggcgtgag tattaattct catggttacc
     1261 ccattcgttt gatcttgagc atttttagca tgcgttgcca atctaccaac ttcttggcgg
     1321 tggctatttc taccttgatt aatatcgcct tcaaccttat cagatttatt tgcgtattcc
     1381 tgtttcagat tctcacccct agcgttgata gcagcactaa ttccatccgg aattataccg
     1441 gcctgatgag cttgggtttg aacatctcca aatgccgccc cagctccgga tgaaacttta
     1501 tcagagaagt gtttataagc ggcttgcgta ttgttttgag gtctatcttc aggggaaagg
     1561 tcattaggtt tccagcctga attttccgca gcagctttta aacgatcttg actatttaca
     1621 cctgcacgaa ttgcagcatt gtaggttcct ccctgtcctg cgtttaattc actcatagtc
     1681 aattcggggt tgtcttgtac caatgcattt gtgttatctt gggcaatggt aatgccatta
     1741 ccataagttt gagctgccat ttctcgataa gattgagctt tttggtagct ttctgatgcc
     1801 atatgcgagg cttctcttgc tttgctaaag ttagcttcgg cagtttgtgc caaactatta
     1861 ccagaattga gtgcaacttt atatgcttct gattgggaaa ctttgtctgt aaaattacta
     1921 tcaaagctaa taccttggcg tttacctgct tcaatacttt ccccaatttc tgctaccatt
     1981 tggtccgtat tttgtgattt accactccaa cctaaatcac cttttgctcc taaagcattt
     2041 ttaatatttt gggctttagt actactgccc gtattgccac taatgcctaa cccagctgcg
     2101 atggcagata taactgctga tgtattagta actccaaatt tttgggcaat cttcttgcct
     2161 tgatctataa cgtaagataa ttttctactt tcagctgcat ccaaacccat tccgtaacct
     2221 tcagaggcac ttctatccat accataattg gttgcaaagg caaggcggtt tgcattgcct
     2281 tgagatacgg actctcgaaa ggaagcttgc cattgttggg cagatgtttc tgctcggtct
     2341 gcagccttcg aatatgactc tgattggcta atctgtgatt gagaggaaac accaaaacta
     2401 ctattcctca tctgcgctct actatctcca gttgccatat tccgccatga tgttccggct
     2461 gcagattgag cagtcatcat ttgaggatcg gtatatacag ggtttaagct atgtttgttg
     2521 gcattgctat tgttgtagtt catattgccg gcagagtaat tccccgaccc tacttgggaa
     2581 cccgctgctt caccggttga tttggccgat gaagtcatgc ccatcatgcc gttcatcagg
     2641 ctgcgtccgc ccatggcaag agcacccgcg atgaccggaa tcagccacat tgcagcccca
     2701 gcagctgctt gtgcatcggc tgctgccatc aataacgtat cacctgttcc cggtgtaata
     2761 acgccgttgg cggcttccac gctaaacaga cgcttcgcat tcatagtcat aatcatattg
     2821 atgatactgg ctacgggtgc ccaaagctga atccatagca tactcatcat gtagaagctt
     2881 aataaccgga atgccatgat gccacccaac atggcgagaa tcattacaaa ggggaataaa
     2941 ccaatcagaa tggcctcagc agcagaacga atcaagggaa gattccaact tgccattgat
     3001 gagccggttt tttgggccgc aatatactga ttacgagctt gaacttgagc aaatgcctgc
     3061 tgtacggcag ccggatttcg gttgcgttgg ccattgataa tgcctgcttg acgaataacc
     3121 tcaaccatcg ccgcctgttc gatatttttc aatgtatcga agcgggcatt atccagcaga
     3181 tattggaatg agctattgcc taaggtaacc agggcattta cccggtttat tcctgccgaa
     3241 tttgtaccca aacctgcaaa aacacgatcg gcaatgtagc cgggcaagta atttttgcta
     3301 taaaaccggg tacggtcttt tagttctttt gccgcctcat tgcaggtcat ggtctttgat
     3361 ttgccgttat actgcaacat gttgcctctt cttgaggcga tctgcgatac attggtaaac
     3421 aaggcttggt tggtatgggt caatgtagcc agaatatcgg cactctgtgc cagatcctgc
     3481 acgttataga agtgatacag gttcatatca aagaatgaac actgatacat gaacagtgcc
     3541 cagtcttgag tcagttgcgc gtccgggatt cgtgcttcac ggatattttc catcaggcgc
     3601 gttccgaaca gcatgccggt gcgtgagtaa ttgaggtcgt ccggcaggtg gttaacctgc
     3661 tcggctttgc gggtaatact ttggccaaaa gaactgacaa agtagcccat ataagcaagg
     3721 ccgactggta cattgccgac agtatattga cggcctgtat atccataccc acctgattca
     3781 gtaatgagta cagtggtttt agggaccatc atacccatcc aacaaaataa cacgacaaac
     3841 aaataactga agttatcctt caaatccaat ttgaagactg caacagcaag agtgaccagg
     3901 aatccaaata aggcagcagc ctgcattgcg ccacccatca tcgtagatcc tgatattgtg
     3961 gcaacgccat tgaaaaccgc ccgtacagct tctccgttac cagggaaagt aaagtattcg
     4021 acagccatca tctctcctta gaaacggttc atcgaatcaa aagtcatgga gttgttggta
     4081 tccacaaagg ctgagcgcca ttgtgtttca aactgctcca tagccctcat catgctttct
     4141 tcgatctcga ttttcttcat tgagtcaatg cgctcctggt aaattctgct tcgcaattcg
     4201 ccaaggttgg caaacaattt tcgaaccgct tccccgctgg catcgtctgt cttgttatac
     4261 atacccaagg tcgagcgtac ctggcgttcc gattggtcca aaatattggc cataatatca
     4321 acggcaatcg catctaaaac cgctgttttt ttcaaatagc cagttccaag gaaagcatct
     4381 gatacagcca ttttcagtat gggcaattta ctgttattga caatggcagc aatctgcttg
     4441 cgcgtagata aattccaacc ttggttattc cttattgcag tcggaagcgt ttcaatgcgt
     4501 ttgtgaacca tgtaccgcaa agaaggtatt tctacatcat ttttctcagt gcagttctcc
     4561 attaatttct tagaaccgtc tttgcacgtc cacatatcca atacaacctt ctcccggatt
     4621 tctcccccgg cattgttttt tccgtttaaa agatcttgga tgctgaggat ttttggttcg
     4681 aaactttgaa tacctactgt gtatttgtcc ttatcccctt catgttgtgt taatggagtg
     4741 aacacaattg ttcccagcat gctgtaatag aagtccatgt cgatacccag tttcgagcgt
     4801 tgacccgtat agttcttcat ggcctcataa gtcaaattac cgccggtaaa gctaatcgta
     4861 tcttggggtt ttccggtgtt tcgcacctta tcggcttcat ggacaaggtt tttagcggcc
     4921 tgacagtacc aagcaccttc tgccggatca tttgacgaac ccgaatccac tgaagcggcg
     4981 cgacaatttg aactggccaa cgaacccagt ttccccattg cgccgccgac aatatcctga
     5041 ctcagtttgc aactgtttaa actgttttga ttaacataat tgaccaaatc acgaataccg
     5101 ccaattgcat cttggatcat cggatcaagg gccttaagtg ccaggtcaaa ggctacacct
     5161 gctgcgttat tgcccaggtt tcgcgtaaat tggatgaatt gttctttatt gacaaacgag
     5221 aaagagccgg caaagaaatc tataccgccg caaccggcac tgattgaagg taattgaata
     5281 ttcccaaggc tgatatccga caccggaaag cgaacgctca taccgcctcc cgtatatagg
     5341 ctgccgcttt gaccgtggaa cgcacctgct tcagtataat tggcttccgc tcccattgag
     5401 ttaaacatgt cagccatgtt cttctctatg ccggcactac tattgatcgg aagggccagc
     5461 gatgccgcca agcatagata ttttgcagag gaagcacaaa ctgtcggcac tttggccggc
     5521 ttcggaaatc gaaagatgtg aaatttcata accgctccat gaagcggtta tagcagggaa
     5581 atccttacta taaccaagat aagttacagt aaagacttga gattaaggga atcttatttt
     5641 acctgaaata tccgcgttgt caaccggtta atacatagaa atataaatat tgaaggcccc
     5701 gtatagggcc tttgatttga ttaatacaat gtcccgacag ggcgggaata cgctaagaaa
     5761 cgatcctcaa gcgtggtttg cgccatcacc ccaaatccaa tgggtgtaaa ttgcttggtt
     5821 tttgtatcca tgacgtacat tgcaggtaca gtagtaatat tcaacctttg ggcaataccg
     5881 ttgttcggca aagcacgcgg gaagtgatta atacctccgc catcgagact gaccggcata
     5941 accttaatac cgtattggtc ggcaaagttt ttcaaaatcg gcgccatggt atgacagtaa
     6001 gggcaatccg atttgaaaac aaacataatg ccgttctctc cggcaagttt tctaattgcc
     6061 ccggcctttt gttgctgacg gttatgatca tattgttgct gggccagttg gttgttcgga
     6121 cgaccgcgct ggctgtaatc caaatttggg ttttgccaaa ttaccctttg ccattgatcg
     6181 gcaaatgccg cagatttgtt catcacaacc tcttgaagtt caatatatcg cttcaaattt
     6241 tgaggtgtgg ggttcatgac ggcaatttta cgcgactcat ccaaatttct ttgcagcatt
     6301 tcaaattctg caagttcatt acgaaccgga gtattttgag gccggttttg tccggcttgt
     6361 ttgggctttt tagcatccgg ataccataag aaagcacggt tttcgttttt ccagatatct
     6421 tggtcatacc atcccatgcc atcatccgca gctcgcagat tggaggcgtg caggctaaat
     6481 atcagcatga cggccatccg cactcttttc attttttacc accgttcttt ctgttcgggc
     6541 acgccccgat atccataatt tcagacggaa aggtttgcga cagtgcgggc gaagtatgga
     6601 atgtcaagcg aaccctgttg catccgggtt tgtttttaat ttcttccatt ctgaccgcct
     6661 cgacaaaaat aggtatgtcc tgccggaatt tctgtttgac ggcagccgca gcacggccgc
     6721 tgaaataccc ttttgccgaa ccgtgacgca ttgccagcgc aagcaaggca tccggcttgc
     6781 cgtcaggaag cgggatggaa gtccttgcta gagcttctgt tttttccaaa gcgataccgc
     6841 cggtataagg aagggatgcg gcaagtgcca tacctgtaaa aaacataccc gatacaatac
     6901 ccgccgacgc agccaaccaa ttattgatat tcataatagt tcttcacttc caaatttttc
     6961 ccattaacgg atgcctccct gattttttgt gaaacctttt gcccctgctt ggccacgttg
     7021 tccatgttgg catagatttc ttcataaaac ccggacagat caagtttgga aaagtctaac
     7081 tgttccaatt cctgtgcggt aaatccttcg catttcgggt tctccggcgt accccaaccc
     7141 cgtttcaatt gctctttgcc gccgacattg actattttgg ctaatttgga aatgaaacag
     7201 cactggctct ctatcattgt tacacatgcc cccaatattt tgctgccgca ataagaaccg
     7261 acataatggc acaaatctgc tttacgcttc atggcaacta aaatttcact ttgcgggcac
     7321 ttcaaccact gttggatggc gattacggca atagcagccg caaatacggc cgggttgaag
     7381 ccgacaatta cccccatacc ctgttgtccc gcagcagaga aacccaacgc accaatctga
     7441 gatgttccgg taataccgcc ggccgcactc agggccgtac ccattgcctg tgctgcggag
     7501 ctgacaaaca tagtggtaaa cgtataatgg ctggcaacgc tggccataaa atacgttgtt
     7561 gccatagttg tccctgtctg taccgcgaag tcaacaaatt tgcccggatc tgcttttgtt
     7621 cgacagcagt ctccgccgaa tacgctgtct aaagcaccat ccagcttgat agagcattgc
     7681 ccggcttctc cattgaaaaa acgctgtttt tcgggatcat aaaaccgttg cgtctcacgg
     7741 gcgaattcca tcgtcgtcat agttttggca aaatcccgat cctgacaacc ggatacctct
     7801 ttggttgtct ctcctttttc ggtacattca aactgttttt ctctggcttg acaggtaccg
     7861 tccggcagct gatgcgtaca cttgtcccca atcagacggc attggtctga ggatgtataa
     7921 gcgctgcatg tttcggaatc gcttccgtcc gtacattgca cggtgcgcct ttctttccag
     7981 caatcacgtg taacgggtac gccgttgatg gtcttggtac tgccgggttc caaacactct
     8041 gttgcaactg ctttacattt ttttccttgt acctgacatg acgactcatc ccattgcgaa
     8101 ctaatcagat gggtatgctc catttcttcg actttggtcg gaagccttcc ttgatgtaac
     8161 gttctcaaat ccacatcgca agaatacttg tgcgtgtaac gcaaacactc cccctcattc
     8221 gactgctcca aacaggtttg tccctgtact tcacattttg ccgaagggtc ttttaacggt
     8281 gcgcaataat cggcagaatc cttctcaagg cactgatact cttctttgta ttcccaacag
     8341 tctttagtta cgtcgacccc gttgatgttt ttggtcgacg ggccatcaac acaaacggaa
     8401 gacgtccggg tacacgctgc actttccctg agtgcggcag cagacgctga agtgattaac
     8461 ataaagtggc agatcataac cagcaaagac gttatagccc tcattgtttt accctcttct
     8521 catactccga acaactgttt tcccatttat cgtgacaaac gggctcgcag tattggtgta
     8581 cattaaagaa gatagcattt tctccgccgc ctccgacaac ggtgcgggtc caaatggtat
     8641 ttttgccctg atgcagatac ggccgtatgt cgatgttcag gtttttcctc caggatttac
     8701 ctctttcagc gttacccaac cgagtacctt cctcattacg taccggtgca aaccttcttc
     8761 ctataggaaa tccatcttca tcttttgcag tataatctgc cgcaaacatc tgattgttat
     8821 agctgctgta cacaattttg ccgttgactt tgacaatcag ccaatcatcg tactcaaccc
     8881 ggctcaaagt aaacagcgtc agtttttccg gatttttcac attgagcgtc atctcccgat
     8941 catactgccc gtcacgccaa tagttgtcgc caatagtacc gaacatcagc ctgtgtgcgc
     9001 ttcccgaagc cgtcagcctg accgtcatat caccctgcag gctgcctgct tctatacccg
     9061 aacgggtaca gtttggtcct gatttctcgc ataccacctt taatttcttg ccgcattgat
     9121 actgctggtt tcgatatttg ctctccaggc aggaatagtt ccagccggga tcgacattga
     9181 ccttaagggc cgcctcgcac aagaacatct ctgcgccatg gtattcattg caacgtttga
     9241 cggtttttac cgcaggaacg gtaatggttt ccgtaggaca tgccgacgag ggaactgtgc
     9301 cgacgctgcc gtcttcattg acgggatatt tccatttgta tttttccagt gtttctgccg
     9361 gatcgcctgc aattttctgc gtgcctttaa ccaacttttc atttgaagat atggttacat
     9421 cgggccgctt ggtacggctt cctgcaatca ggtttacacc ttcgcattct tgacgaagat
     9481 agagatccgg attggcaggc agatttttac agtcgttgaa tttggcgtca ccaaactgag
     9541 tcggaagaga aactccgcca ccgtaatact gttcagccat tggtacgttg tctgtatatc
     9601 ttggggtata ttgggcaggc tgcatcatgt ttgttgcagt cccggccccc aattttccca
     9661 gttcgccgac gtaactaccg taatctgcag caaaggccgt ctgcatgagg ccaaatacag
     9721 ttagataaat ggcatgtctt ttcattttct gccttccaga acggctaatt tcgcatttgc
     9781 aatatcggaa agttttttat cttcagactc acggatcaag tgttccaaag ccgaatgcaa
     9841 ggacacatca ccctttacgg caacatgttc cgtacacatc cccaattctg aatttacagc
     9901 gcaaccgtct aaaccatctt cgctgtttcg ggcgattacc gtaacaggga cttcggtgat
     9961 gccgtaatgg tcgaagagtt cgggatgaat cagcatatcg ccttgcaaag ccgcaagttc
    10021 ttcggccgcc ttaaccgtgg ctttcagtga gtcgtcttta aacccgcgga taaccattac
    10081 cccgcctgcc tttgctgttt cacgggcaat gcgtttcaag ctggcttcgg gcattgaaaa
    10141 tgatacgaat accattaaat ccgctttttc cgcagcctgt cgggcttctt gtgaggcagt
    10201 gcggtatttt tgtgcgatag acatcggatc agccttgatt tttgcatttc cgtaatcacg
    10261 aacatcgggc acacggtgct tgataccttt caaatccgga atttcaggtt gctgccgatt
    10321 gtcacccaca tttgggtttt taactcgggt aactttaccc atggttgtac gcagacggtt
    10381 tcgcgccgcc tctacatccg catcggcgta agcactgcct actccggcca aaccgcccaa
    10441 aagaatgagg aagacgcctc cggctacgat tacattgcgt aaagacttgt ccatcactcc
    10501 atctcctgtc aattagtctt aaatttatat gcaccgcagc agtcgcgctt acggaaaagc
    10561 tggaaagcaa actgattacc tttgtatgga aatgctttgc cgctgtctct gactaatgtt
    10621 gaacgtccaa taggctcaca gcaacggcct ctgaattttc gggcggccac cggataggtc
    10681 atagccattt tatagttctg tttatccatt acgggattaa gtttgtaccc gcataaagca
    10741 tccctgcctg atgtcctcca agctactaag tccctgtgca tttttgtctg aattctagcc
    10801 gccatcaatt tagcggcctg cacttcggag aagtggccgg acatccatcc tgacaaaggg
    10861 taaatcgttc cgtggcaacc tgcacaccaa tacaatttat tgaatggaaa gccggcggcc
    10921 gctttaacac agtcagccgc acaggctgct tgtgcaacga cattggcaaa gagcgcagaa
    10981 tcaggggctt gataaacggc caatgacgga tatttccaag tgggatcaag ttccgtcatc
    11041 caaccgatat ccaaagtacc tttttccata caatcgttgt ccaataaggc ttccaaccaa
    11101 aatacgaccg gatttttata ccaatgaact tgataaaagg cattatgctg atcatgttgt
    11161 tcgttacgac cacgcttatg cgagccgcca aaaagatttt gaatcttatt ggatacgtct
    11221 gctttacctg ttgtttccgc cgtatctgct ttttgatttt taagtgaaat accgcctagg
    11281 ctaaccatac agaagggggt gcgtacgact tctaccaagt gtgtgggttc ccaaaatgat
    11341 accgtcacgc ctgaacggct cgggtgcgta cacgaacaaa accaattgtt tggagaagag
    11401 ccgttatcct cttgattatc catattcaga ttgattttgc cgccgatttt gattgggaac
    11461 atacagctcc aacaaatatc atttatcgga ttaagcatct tcccctggca ggttaattta
    11521 ttcggtatcg gagcagtagg aacagcttca gcggcgtgag atatagaaaa taatgggagt
    11581 agtaataacg gaatcagtaa tttcagcggt gtgacaaata ttttcacggt ttcatgacct
    11641 ctacacggag ccggccccct tcttgggaga ccaaacttgg aacttcattc agctgaaaac
    11701 ttttggacat ccgcccttct tggtcgtaat aaaccgcctg atccatttct actgataact
    11761 cataccaatt gcctgcagtc agaataggca tgaatgattc gccatattct gctgcaagtt
    11821 tcttggccag ttcgatttgg tcaggttccc ggccgtcaaa gaaaaacatt ttgaatggga
    11881 ttggggctgc ttccaaaggt ttgacaacag taccggcagg agcaataatt tgaccgtctt
    11941 ggtcataaat tgtttcggtt aaggtataac ttgggtcgaa gtatcgaaca gagcctttag
    12001 ttgccttcgc cagtccttcc aagggctgta gatttaaggc ggagcgattt attctatctt
    12061 ggacctcttt ctgcattgcc gccaattggc cgctgtcttg tttagctttc aaacgcgctt
    12121 caatttcgcg gattgcatgt ttttccgcta ttgggtatgt ggtgccgatt ttgtcgccaa
    12181 ccttctcgac agagggtgtc gaagcggcta cccatgagga aattccggtt gcaagtatta
    12241 ctagcgatgc gactgaagct atttttttct tgtgggacat actcactcct aaaaaatggg
    12301 gaccgcctta ccaataattt gccctgaatg gattaagccg gataattcat atcgactgtc
    12361 caagctgtct ttgtgccctg ttttaaccca catgaaccgt tgtggaattt ccccttcgaa
    12421 tgggttgatg gccagcttca tgccatccag ggagtattct tttgcagtac ctacctcttt
    12481 cccatctatg aaaaaagtac gattcgcctt cgttacatga tttccgggaa gtccggcaac
    12541 ctctttgacg acctgcgtac caatagggta aaaaccacct tgccatgcaa aggccacata
    12601 atcgccctgc ttcagatcac ttagcttgtt cgcatctttt ttgatgaagt aaagatggtg
    12661 aggcaaggaa cgggaaagat taaatgccaa agcataatgt tgattgaatg ccgacaagca
    12721 gaccgcaact gctaaaaaag gggttgcata tttctgacaa aactccctat aaagacgttt
    12781 catcactgct ttgttgcttt ctttttgtga tatttttatc attgcctcaa acattaacga
    12841 tccatttctt cttcagtaac cggggtgcct ttaccctctt cggccatcgg tttcggaatt
    12901 gtcttttcaa cttgttgatg taccggtgcc gccactacag ctgtggccgg gacacgctta
    12961 tccaactctt tcataatgtc agcagtaaca tcaaccacct ctgtgttccg tccgaccaat
    13021 gcagatttga caaagatgtt acagccgcac cgaattacct tttcttcgat aacctgttgc
    13081 aaggtattca tctttgcttg gtagttttgg aggatcgcgg ctcgttgccg gtcggaaatt
    13141 ccattctgca gtccttgatt cttcgcctgt tcccggtaag tcatggagat accggccaaa
    13201 tctacaatat ggaccggttt ctcatgggtt ttcatgaaat tccatacgcc taatgccaat
    13261 gcggcaatag aaattgcgcc gatcacaagg ctgcccaatg aaacctctac gccgttatcc
    13321 tccggattct tttccggatc ggtatttgcc gtgttttctt tcttttcttt tgcttcagtc
    13381 atcttattaa tatcctatcc tctgcctaat tagcgggttg cggcacggtc ccttaaaact
    13441 gcttcaatgg cctcggctgt attcaacccc tgatttctgt aatggttcac ggcttcaacg
    13501 tcttccgctt ttgagcttga ggcaagaacg ttgaatgggt ccgagtacaa cctacctact
    13561 ccataaccta tcggggtaga gacaaatatt tcactgtagt agtcagtttt gctcaaagtt
    13621 cgcagaatct gcataatccc gtcattgact tcaaaaggtt ctttgtcttg aatggttttg
    13681 agcgatttag ccccggtacg caattggaaa acaaagtcgg cattctctaa tgccgcctca
    13741 gccgccttag tcttgtagta gtcatttgca ccttgcgtca gagtgccgca tgcacctttg
    13801 tacttacgga cacggcggta gccttcctca ataaaacgag cggctgtacc cgtgtcgccc
    13861 aacaaagccc aagcctcgtc aatcatcata attttgtact ggcttcgtga taggtacatg
    13921 ccctgcataa tgcggtacat aatcagctgc atgatgatct gttgcagatc cggtttggtt
    13981 tttaactctt caagttccag tacgaccagg tcatttttaa actcgacatt caattttcca
    14041 ttgaagtaac tgccgtagat accttccgaa cagtatgtgt acaattggtc ggcaacggcc
    14101 accatagaca aatctcgttc accgggcttt cctccttcat tactgccggt aatcaaacac
    14161 ttgagacagc gttcccttac atgatcgacc gtgccttctt gtccgaattc gtaccatact
    14221 tcttcgatta tccgttgaag cttggcttcc tgctgttccg ttagggagtc tgacggagag
    14281 gccatcaatg agaagattag tttcaacatc tcaatatctt cgttgatgtc cgttaccatt
    14341 tggaatggcg ggaagcacag gcctgcttca taggtaaatt caatatactg cccgccaatc
    14401 atttggcaga atttttcata cgaacggcca acgtcgataa tccatacctg agtccccagc
    14461 cccatgtagt tgcgcacaat ttcattggca aaaaatgatt tgccggaacc ggaagcgcca
    14521 ataatggcaa agttatagtt acctgccggg ttggcaaata agtcgattcc catggcctgc
    14581 ccgcgttttc cgaacaaacc gatgacaggt cttcctaaac cgtgccactc gccgataacg
    14641 ggagacatac agacagcatt agtcatggtt ttggttgacc atcttttctt actgtccaaa
    14701 tatttctcaa cctctttgtc gatagccatt gggagggcag tcaaatatga ccccagttga
    14761 agatattggt cacgataaag accgaagccc tgagcctgcc aaatcgatac cgcctggctt
    14821 tcagccaagt taatgttttc gggacgctcc caaatgacaa cttggtgcaa caaactcacc
    14881 agaccgccgc cgcctttgcc gaacgcttct aggcatatgt cgtaatcacg tttgattttt
    14941 gctgcctccg gcataactgc ggccatcttg gattccgccg tttgtttggc gcgggccgct
    15001 ttcagtttta cggtattgct ggaagtgtca taatcacgct tgaacatcgc catggaaatc
    15061 agaaatggtg atgtgtaatt catgtttgcc tcaatgacat caccaatcaa tgcgcccata
    15121 ttggacagat gaaactcttc agggtagcga ttggcagaat agcattgagc aatcacgcaa
    15181 tcccccgcgg cacggttgcc aaaacgcagc cccgagccgc tatcaagaac cttgatagat
    15241 gtgcgggtct caatcatttg ttcactcaag aacttggttt catcccaaac aggatcgatt
    15301 tcatcttgtt gtccggtaaa catcctatta gggttgagta ggagcgttgt ccaagccaat
    15361 aaatcgtctg cattccaagt tcggccgtaa atctgcatgg agcggagtga atcgcgcatg
    15421 cgggcaagga gcgaaaccgc cttgctgact tccactgcat tatcaggtgg aatattcaaa
    15481 tttacgctga ggatacagcg cagatttcgt aaacgaatgg ccgtattttc taccaagacc
    15541 ttctgtgtac ccttattcca aaattcagta cgttttttgg ccaaagtttc gaaagtgctt
    15601 cgcatcggat cgtctatagg tcgtgatgct tgtacttgct catattcctt gaagtaattt
    15661 cttaaatcgg gggagccgaa aatttgaacc tgtatcggcg tcttttcagg aagcagcgca
    15721 tacaggctgc cccacttacc gatcaggtcg tcactgccgc ctaaaaatgg gttaagttcc
    15781 agaataaagc ccatgcccaa cggtttgtcg cctttaacgg acatcagcgt aaacaactct
    15841 gttttggtat cgtatgcacc gtaattgaga tagtcgctca aatggtcaaa gtcaggatcg
    15901 acagggttgt tgtgcaaggc ttccatcggt tgatcgacat catccgaacc ggccaaccaa
    15961 tttttaaatt ttgacaggac gcccattaca tacctctatc gaacggtacc gggaataccc
    16021 gatgtttcct gcagatgaag attcacgcca acatcgggga tgcggtcatt tgtgttgttt
    16081 gaattggttg gagaagtctc aaccgaactg cctgaagttt tggccccatc ccccaataag
    16141 cggatgggac gatattcatt tataatctgt tgcccgttgt gggcaattaa ccaatcaccc
    16201 tgattcagaa cgacatatgt gaaactttgg tcatttaaat cgccgtccgt atcaagatag
    16261 ggagcgaccc aaatgcgagc aatttcactt tgcgagcgaa cgggcgtacc ggaggaataa
    16321 gcttgcgtta acattaatgt gcgaacactt tgatcagtgt aaggttgtcc aacctgtctt
    16381 ttcataccgt cttttaaaac acctgcaaca gatgctttat agttactgct tatagattca
    16441 caataaggat cgtcactgtt ccgggcatta gggcatcgga atttttcact accgccgata
    16501 ccggacatgg ttaaggttga acatccgcta agaatcaata ttgccgcagt aatcgataag
    16561 gctttcaaac gcatcattct gctcccgctc catttaacca tttttccaat tcggcacgcg
    16621 gcatggcacc ggatgtacgc cgaccatctt tatgaatcat gcttggagta ccgcgaactt
    16681 taagctgctc tgcgagtgca atattttctg atacagggtt tttacagtta ccggccgccg
    16741 gttctttatc catcagcatg tagttattcc atgctttttc acgatcttta ctgcaccaga
    16801 tatgttctgc ttttgaaatt gcatcagggt ggagggattt aatcgggaac atgaaaacgt
    16861 atgcggtata gtctgtcatg ccggccaatg tttcttccaa acgacgacag aacgggcaat
    16921 ccgggtcgga aaaaagtgca aattctcgct tgcctttgcc gccattacct ttaacgattt
    16981 tgatggcgtt tttgaatggc agtcgggaat aactcttttg tgttaccgct gaacgggcca
    17041 tctcggtcaa gtctttctgc tcttgcatat cgtacaacgc gccaaaaatg aaatagcgac
    17101 cgttttcttg tacataggca atgttgtcac ccattgtcac ttcataaatt gaagccatgg
    17161 gggtagcctt tactgaagaa aacgtggtat tcggatacaa cttgtgcaga gtgcttccaa
    17221 cagttttgac atcggcaaag gccgtctgaa aagctgtgat tgctgcaata gtggccaaga
    17281 ttttcttttt catagatgga tcctatttgg tttcgccggt atcaatttcg ataccttgag
    17341 tcagtacgac atcgacttga cggcctgcat tgacttcaac taccgggaag accttatcgg
    17401 ccagttttat gtaatactca gcaagcctgt cagctgcacc accgatacca gatgcaatcc
    17461 ctgcacgata ttgatcactg ccccgaatag aggttgtgct gccaaatgcg gtattattcg
    17521 tcaccttgaa tgcttccgat acgcccttac ccataccgct aatcacgccg gacatcagtg
    17581 catttgccaa gagttggcct tgtttcgtta caagattacc tctcacgcct gttttgccgt
    17641 cttcaccggc aacgtaacct tttaccggca tgtcgatggc tcggccatcg gtacctacac
    17701 agctgagatt ctgcagtctg atgtaagcgc gttctgaaga tatatcgccg taagcctgcc
    17761 cggttacgaa acattctctg aaattgtatt taaaccggtt gggaagttgc gcaaaatccg
    17821 ttacgcgcat taaaacaggg tgggggctgg cattttgtgc ctcacccccg gtcggtgcgt
    17881 caaccccacc taacagtacg gatcggaaaa aagtaccgct aggtatatac gagtcacgcg
    17941 cccgaaactt gccttgcgag acgacaacct taccgccgtc ttcaactact tctatattca
    18001 tacgcggggt ttttgattta acccactccg cttcttttaa ttgcggggaa acccgtacat
    18061 tttcattcgg atttccggta tttaccgatc cggtcgttcc aggttgcagc tctgcttcac
    18121 tcatacttcc tgaaacgatt ttccgttcaa tgggcctatc tgccggaggc gccccgttcg
    18181 gcaatgtgcc tgccggaggg gccggaggaa gccggccgtt atccccgata ccgctaattg
    18241 caggcggttt gcctaatcca tcaggcccca gccccttact tgtatcagga ttactgctgt
    18301 tctctttttc ttttaactcc tgaaccatgg tttccaattc gctgattttt cgatttgcat
    18361 attcaatctt cgaactttct gtgttcatcc ataaggaatt gtccgtaatg cctgcttggg
    18421 ggcttgtaaa atcagttctg agaatctgcc gtttgttttg aacctgtgtg acttcggcag
    18481 atttcttttt attgccttca atgacataaa ttgcactgcc cacaatcgcc gccatgctgc
    18541 ccgcaaccat gcccgcgatg gctaactggc gttttcgcgc tgctgaaagt ttaaatttgt
    18601 ttactttcac cctcatctca acctcccgga cggctgataa taaatacgtc ggtaatttcc
    18661 ccgggttgta ggatctgttt ccgaaccgct accgccaaaa ctccttgtcg gtaaaattcc
    18721 tgctctctaa tttcaagttg tttagtacct aaattagtta atgtaaaaga aaggccttgc
    18781 atatcagcgg cggtgtaact tccgttttga ataaagaggg tgtttttcca taatgggacg
    18841 gtttgatatt catgactatg attgatgccc atatcgccgg tagtgccacg catcatagag
    18901 gccatcattt ttttgatggc ccgtacatat tctgtactat tcatcgttac aggcattgga
    18961 ccggtttgcg aagccaatac tgctgcttca cggcgattag ctccagccac atcgattacg
    19021 atgttgtcac cctgttttga tgtaggttta agaaccagca aataggtctt accttcttcg
    19081 cttataacga aaagatttgt cggattggag gtcaaactac ggacaaacag ttggcctgtt
    19141 gttgtatctt tctgaagctc aagatcaccc tccataaatt tccatgatga aattcgaccg
    19201 ccttcaatgg cgattctgct caagttccgt ttacttattg aaacaacgtg aggtgtattt
    19261 tctgttgccg gcactcgttg cgctgcaaaa ctagtcgcgg gtccggctgc caacaaggca
    19321 gaaaagacaa ttttttttaa cagtgaacac atatcaatta cctcgattac cgcctttaaa
    19381 ttctgttgcc gttttactga tttctacatc ctcggtttca acttcggatt tttcctgacc
    19441 tgcgggttct gctttagtta cacgcttttg aacttcttta atcgataata attgcagtgt
    19501 tccataatta tttttgaaag aaataagtag ctcgcggtct ttttgctcta ccagggcatt
    19561 gttgcgttta acctcaaaaa ccccacgaat ggaaactgta ttgtctgaaa tgtccgccgt
    19621 tgcttctgtc gcgctaaaag tggtgccgac attatttttc ttaacccata aggcatttaa
    19681 agacagctcc ctctctaggg catctttata atcaggtgaa acaatcttca ataattgact
    19741 attctgataa tcgactgttt taggggttac atttaagaga agataggcag catagggagc
    19801 catttcttca agatattctt tagaaaaagc gatgtttgat acagtcattg accgccggat
    19861 ttccggcggg gtcagcgtcg tttgaacaat cgtacctttg aagagcaggg tcgcggacaa
    19921 aaatagattg acagctatag acattcccaa aagtatccaa aaaaagacct ttatgccggt
    19981 tcttatcgcc aattcactca acatatcttc agtcttcaat ttagaaactc ccgtttattc
    20041 gtttcaggta tccgtttgtt tttttcatta atgggtaagt accagtaaag aacatgcaaa
    20101 aaaaaccatt gatgcttgcc agacttaagc ttgccccacc tccatgcaat gaaaaggcta
    20161 attgacatgg aaatgaaaaa gtgacgagag acaataccta taccgaagcc ggacataaat
    20221 aatataaaaa cgtccaagtc ccaaataaat gcctttttgg gatcgtctat cgataccggc
    20281 acatgataat ctctcatcac ttctcttatc tagtttgatt aaataatcaa ggtaaacata
    20341 cctttaatga tgcccgggcc taatgttgcg gccaagccaa cacccaaacc gacgatacat
    20401 ggcatgatgg tttgtttcat caggcctacc gccaaaccga tcaagaatgc agtcaagccc
    20461 aaagccatac ccagataacc ttcagaccag cctgttacca tgtcagccaa gcctttgaat
    20521 tcggcacctg ttgtagcggc cattgccaag ttagcataca atgccaatgc aaccgcacta
    20581 gcggacttta ctaatttatt gcgtttgata ttcattatga acctcattaa acagagctta
    20641 tatcgccaag cttttgcgtc cgatcattct ttaccaagtg attacaacgg ttctcgtatc
    20701 atcactattg cagcaggccg gggaacttgc tgttcttatt tttgcggata cattatgttt
    20761 cctcagccat ttacctatta ctgctgcccg ttgtttagcc aaacgtctat taattgcagt
    20821 atttcgacct ccgatactgt cggcccatcc gcgtaaataa accgtctgag tatttttcaa
    20881 tttaggcaga acacgtttga gcgttctcac gccctccgca ccgaggtgcg aactgcctaa
    20941 attgaaaaac accctgatct catctttttg aagattggtt tgtgtttgag cataaaccgg
    21001 ttgttccgcc aaaaccttgc gctcaaatcc cgaacactct tcattgcggc agaagacaaa
    21061 ttcgcccagc tgattttgtt ccactgtata agttgcgtct tgaggcagcc cggccacttc
    21121 ctgttttggg gcggcacagg ctgtcagaat cattgcggag gctacgaaga gcgtgttttt
    21181 aatcgtttcc aattttgata taccttattt gcgtaagtca ttcttgcgcg atagcacgcc
    21241 cggcctttaa gtttggtaca tgcggcgtta taagcaccaa ctgcttccca tgatttccca
    21301 tgttgccgat aactatttgc caatatccat gcaccaatat gtacatttgt gcatggattc
    21361 catacatccg cccctgttaa accaaatttc ttgtggagta cgggtagcca tgaacggttg
    21421 atacccatca agccaatatc agctgtatga ctttcaatat tcggcctgaa agcgctctcg
    21481 gttaatgcga tcgattgcaa catggcggga tcgatgccat atttgctgcc ggcttcctta
    21541 taacaaaaag caactgctct tgcagatgtg agtaccaaaa cacccggtac gacgaactgt
    21601 aaaagtatgg cccttaatct catgtcttta ctgtcgttgt atgtatgact aatttttatc
    21661 tcgattcgac aatttttgtt aaatcaaacg aaattaacta aattttttag aaatgtttct
    21721 ttaaagatag attatggtat aattaaaagt tacgcttata ttttatgaaa ttaccgattc
    21781 ataacatatt gatataaaaa ggaatacgat aagggtaaag gctcttatcc aaaagccaaa
    21841 aggccgtctg ctaaaaaaaa tcgcagccgg ccttttttaa aagctgtaac ggtaaagcaa
    21901 cagccttaat ggttaaagga agggttcaag ggaaaaaggg taaaagttaa aggtaaaaca
    21961 aaggtctgca tgtaaaaatg caggcctttg cctattaaaa ggggcagcat tataccttat
    22021 aaatcttatt ctgtgacaat tctaattaaa ataacatttt ttattttgat ttttgacatg
    22081 gcaacttttt tttattatat gaatggtttg acaaccctgt ctttttaatc aaaatgaatg
    22141 aaacagatct tcccaagctt cttagtgtca taaatcggtt tacggatatc gataaaaact
    22201 ggtcttgcgt gttgttgttc tggatacagc gtgctagcgg ttatctggaa atgatgacgc
    22261 tggatgacga tgaaaatacg gcaaccttcc ttatcgaaaa actagagaaa ctactagagc
    22321 cactgccgat agatattgat aacacgactt ttgcagaagc acttgccgat gactttgaaa
    22381 tcttatttct aatggcccaa tacggttctg aatactggaa ttcacttgaa gaaagctttg
    22441 aggaattttg tattagacat actacgctac ctaatcaatt gattgctgat attccacatg
    22501 caaaaaagga aaaagtcacc atgtggctaa aatctttgct taaatcatca tgaaaacaag
    22561 ccttctcact attgcatcct cactaatgct tgtcagcggc tcaatgctat acctgataaa
    22621 tacgagaaaa agtacagatt ccgtgtcaca taatgatgag gttgcccata tcaatcagaa
    22681 catagtaaat gaccggtttc gcattttaaa cgcgcatgaa ctgattcagg tgctcgactt
    22741 aagccctcaa atatccggta ttaagatgaa cttagggtta tcggatgaaa actggagtaa
    22801 ggatgccctt ccctttttgg aaaaatatat agcctttgtt caaaggcttc ccgccagtga
    22861 gtctcatcac catgcaggtg atggtggttt agtccgccat acactcgatg tggctgcact
    22921 ggctcttgtg gcatctacat cgcaatcatg gcccccaaat gccaaaaccg aggaaatagc
    22981 gaagaaaact gccgtttggc gttatggaat catgtgtgcc gccctgcttc acgacgttgg
    23041 taaaactgtg acgggatttc aggttgaact gtttgattcg gctatcagtc tcgaaaaatt
    23101 attatggttg cctgatacag gctccatggc tgaaagtggc aagctttatt atcgtgtcga
    23161 atttcctgat gcaaagtctg cttacagtac acatgccgaa atcgcctgga cattctttca
    23221 agctttggtt ccttcacatg tcagacaatg gcttgccaca acggatccga atttaatgat
    23281 tactttgaga aattatctga gtgggaaaaa ggatggcagt cctcttgagc agttaataaa
    23341 aaatgcggat atgacctcag tttcaaggga tttacgaagc ggcagccgtc aacgcttctc
    23401 aaccgccaag cgtaaaccat tcattgaaac cattatggaa acattgaagg agatgttatc
    23461 cgatagagga gtccatttca gcatcgcaac aactgccggt ggtgacctgt tccgtaaggg
    23521 tgatcgcatc tatttgatgt caaaaaacgt acctgactac atccggcagt atttgaggaa
    23581 aaatcaacat cctgctgcag gctcatttcc ggctgacaac caaaggatat ttgacacatt
    23641 attcgagtac cgtgccgtca ttccgccgga aaacgatcct cacagggcaa ttaaccatat
    23701 agaggttgag tttacccgta tggacggtga aaaggtccgt aatatcttca gtgtactttg
    23761 tttcaatgcc aaaaccctgt atccggacgg agattatccg actgagttct taggtaatct
    23821 ttcagaagtt tccgccaaga aggaaatacc ggatgttgta gtatcagagg ataaagtgtc
    23881 aactcagata caaggtgccg aagataaccc agtcactatg accaaggaca aaatggactt
    23941 cctgtctatc caaccggtga cagcagaaca acctccccta cctgaaaaaa atcaaactga
    24001 gacagctaac tcggcttttg acaacagtat gaatactgct gctccagcgg aaaataaatc
    24061 tagcgagtca gagaaagcta aagaaccggg ttacggcaca attgataatt taattgaaaa
    24121 ctttaacctg atagaagctg attcagaaca aacagaatca accaaatcag aagccgagac
    24181 agcagaaaat gccgttaacc tggttcaaga aaatataaga gtagaagtta aaactgagac
    24241 agtggacaaa cctcgcgctg aggttaaaga aactgagaca gataaagcac caaaaacaat
    24301 taaatcagga accaaagcaa gccgtaagaa acttgccggc cttttcgcag acaatcgtaa
    24361 acctattgga aaaacagccg aacccgatgc acagattaaa acaactgaga cagtaactca
    24421 tcaaatagat tctgaagcag tttcattgcc tgaatctgaa tcagcacaaa acggtatgcg
    24481 tgaactggag cagctaagac taagagaacc tacatctagt ccacgcccgg ttcaagtcat
    24541 ggatgactcc gaaggtttag atagccttgc aagcggggta aaaaatatgg ccgaactcga
    24601 agccatagtt tctgaacgta atttgaaaaa gcaatcagaa acagagtcga ccgaaatagt
    24661 cgaggattca gtcaaagcca aacatatgga ggccagagac cgtggaatgc agtttttacg
    24721 atggctggca gatggattag gagacggcag catagcagtc aatagaagtg gtgcaacagt
    24781 ccactttatt gaacagggga tgcttttggt tacgccagcc gtatttcgag attacgccgg
    24841 cggcgtgttc aataaatctg atacggaaag tctaggccct cttacacaaa aaggctttga
    24901 ggctttgagg ttgcatgcaa gaacgaaaaa aagctcattg catagggttc tgactacaaa
    24961 cggtagcaat cgcagactct tctattgcta ccttatccct gaacataata tcaaacatat
    25021 catacagcca ggttcaagac ctcagaataa taccgatatt aaattagatg aatccgactt
    25081 attagtaatg gaacaaaaat gagtgcccac ttccctgaaa acgtttatcg atcaccctat
    25141 gaatggatca gtgtagccgc atactccacc acagcggttt cagtagcaca tttgcccgta
    25201 cctccgacaa ttgcttatgg cagtttagct attttttcgt tggtttcagt caaacgtgcg
    25261 ttatcagcat tacgcattat gcgtttccgt aaaaatattc ggaagttgcc ctattatgcc
    25321 cttactgccg atgaaattcc ttcttctaat aaagctcttt ttcttgggaa aggttttaga
    25381 tggacacaga cacataccca acgtcttttt atggttcgcc agcctgagaa cgacggactt
    25441 cgtaacccct caaaactcta taaggcagcg cgaaaacatg agattgagac aatgggtaca
    25501 gactggattt cccgcataac tcggaaatcg gcatggtgga atccggttgc tcccttacct
    25561 ccggtcggag gttctcctga attacatggc attgaaattg aggaagatga tatttgggaa
    25621 gatttggggg agcgtgttgg ccacaaggtt gtattgggca caacccgcgt tggtaaaacc
    25681 cgattatgcg aaaccatgat tgttcaggat attcggcggg gggatgttac gatcgcattt
    25741 gatccaaagg gtgacgctga cctgatgact agaatgtatg tcgaatcgca aaaatcaaac
    25801 aggccgtttt atatgttcca tttgggtttc cccgagtaca gttgccggta caaccctatc
    25861 ggggattatg gccgtattac cgaggtggcg acaaggattg caaaccaact gccttctgaa
    25921 ggtcagtctg ctgctttccg agattttgta tggcgtttcg tcaacgtttt aacgaaaacc
    25981 atggaaggtt tatccattaa accaagctat gaaaatatct ataagtctgc tgcaaatgta
    26041 gatgaacttg caggggatta ctttaaaaag atattagata aacaccaccc tggttgggag
    26101 aaagacttcg acaattttga tatgcccgag tctgaagcaa aaagcgcagt caaaactggg
    26161 cgcaacttgg acacaatgaa gctcggttct ttcttgaagg ccaaaaagca taacgaacct
    26221 cttattgacg cattgggtgg tattttgtcc aatgacagat cctattttga gaagctagtc
    26281 agctccctgt atccattgct ggaaaaactg acgactggcg aagttgccaa gcttatcagc
    26341 ccggatacag aggatttgtc agatccaaga atcattatgg actggcgcaa agtcatggaa
    26401 tcgaatgccg tcgtctatat tggattggat tccctatctg atgccgaagt tgcagcagcc
    26461 gttggtaatg cgatgtttgc tgacttaacc tctcttgcgg gtcagatcta taaattcggc
    26521 catggggttg gacagagtgg aaaaacccag aaaagaaaag tctcaattca tgcagacgaa
    26581 ttcaatgaat taatcggtga cgaattcatt ccgttattga ataaggccgg tggtgccggc
    26641 taccaagtga cggtttatac acaaacctgg aaagacgtag aagccaaaat tggttcccct
    26701 gctaaagcag gtcaaattgg cggcaacttg aatacgatga ttatgcttag ggtgaaaact
    26761 gtcgaaacag cagagatgct gacgaatcag ttgccggaag tcaaaattat gaccagcact
    26821 ctagtctcaa gggctggtga tgttgccttc cctgatagtc acgaagattt tagcagtgga
    26881 aacgaagaca ggattggtgc tgagacagtt cctatgctga cacccgccga tttaacccaa
    26941 ttacccaaag gtcaagcatt cgcgcttatt gaaggtggcc agctatataa aattcgactc
    27001 cctctaccca aaaaggacaa gcaggaggaa atccctgcgc atattggaga aatggcggct
    27061 gatatgcgcg aagtttacag actaagccgg ccttcggctg aagaaaacgc atttacggag
    27121 ggaagtagtt atgccgtcta atacaagcgc atggtacatg aattcattgc ctttaaagtt
    27181 cttatttctc ccgtttaaag ctgcttatac cttattctta attgtgctgc ttatctttgg
    27241 tagttcggtg ttgacacaaa actattttgg taccggtaat ctcttggaag atgaaatcaa
    27301 atggtctgag tctgttggcc atgatgcgtt tccaggaaaa gaggttgacg atactataaa
    27361 tctgcaaatt gccagactta gttacaaggg gctgaaaacc gtattttttg acctgacatt
    27421 agtcaatgaa tcactgaaag ccaagtcaga agccgatttt ggttacaaat ttaaaaatta
    27481 tttagtcccc cgtatgaaat atctacagca attcgatact acgttgaagg ttatttcctt
    27541 acgtttggga aatttggcat taatcgtggg tatctcagta atctttgcaa tcccggtttt
    27601 gatagatggg ttagtgatga gagctatcag gcaggaaaat gcctcaaggg agagcgccgg
    27661 tatttatcac cgtgcaaaat attggagaac agggattatt tggttgggtt gcatgattta
    27721 tatgtgtgtg ccaatatcaa taccggccta cttgctctat ttacctctgg tagcagcaat
    27781 ttggatgatt ttcatgcaag ctaaatatct taagaaatat ctctaggctt ccagtggaat
    27841 ctaaatagtg cttttttagt tcattaaacg ggggcttaat gctttaactg acggtacaga
    27901 aatctcgaaa agcattttgc aaaatactta aaataagagt atagtaaaaa tgaaatattt
    27961 caagtttaac taatagttaa caatctagag taaacaatcg taatcgttaa atctgggtat
    28021 agattgcata atatacatta tgcgaactat cggaaacagt tgtacgtgta gccggtattg
    28081 gggaatacgt tgaccttcaa tacgattaca ggcagccgac acacaggata tgccgcccat
    28141 acttttttaa tccaaaacat tatatcaacg ggcgcaaaca gtaaatcgcc caacttatcc
    28201 caaaaattct cctgcttttt catcatttct tctgtcaaat ccttggaacg gtcgatttga
    28261 aaacacggcc ttacatacgt cccaattctc tttgcagtgc ttggatattt tgctgtctgt
    28321 ccaaaacatt gctttgcaat gcgttgattt tttgatggtt gatgttgccg ccttttgcca
    28381 gacgtgcttg tgataacatt ttttgggctt cagtcagggc tttgcgttca ttgcttaatt
    28441 ctgcttcgag aatggagcgt ctgctgttat ttacaggtgc ttgttgaggc ggcgtattgg
    28501 attttgccgg cttggatact gttttgaccg gggctttata tttgacagcc tgtccgccgt
    28561 ttgacggtga tggtgccggt tcgggagttt ggggcaggat atagcgttcg ctgctgtagt
    28621 tgccgattgg gggcaaatcg gttgagtggc agcttttaga cggcttggtg gtgtaaacgg
    28681 tttctccgtt gattgtgcag gtatagattt tggccgcatt cgcacccaat gggcttgaaa
    28741 tcagggaaag gttgattagg attaagggga gttttgattt cataatgtgt ttgatattcg
    28801 gataataatt tgattttttg aggatttttt gtaaggtttt cttggcgttt tatgggcttt
    28861 tcttttcggt tgtttatttt tgattttcct tcggggcttt ggcagcctgt ctgttctttt
    28921 tcccggcatg ttttttgttt ttctttttgt ttcctgtgtg tattttggct tcttgactaa
    28981 gttttttaat tttcaggcgg tatccgcctc cctgatggct gctgatttta ggtaaatcag
    29041 catcggcaca cgatcctgac gggtctgatg tatatacgct tttgccattt gagttgcaat
    29101 ggtatatgga ggcttgcgcc gccgcgccgg aaagggacag gagacccaag gcggcaaaaa
    29161 gtctgatgtt tataccggta aagtaaaaag gcggtggtaa cgtccgaatt ataccgttat
    29221 tggcaggcaa agataagcac cctgcccgcg cttctttatc gctcggcaaa ctgtttctcg
    29281 atttcagttt caatccgttc caattcttct tgacaagcca gccaagtttc ttcgatttgg
    29341 gcaagctgtg ttttgacttt tgccagctcg gataaggtat tttgcaattt ttctttgttt
    29401 gcttcaaggt aagcgtcttc ttgtgttaaa aatgcttcgc atcccgtctg aatttcagaa
    29461 agctgcacca tttctttttc ggctttgtcg attttctgtt gtatcggctt actgcgtcgg
    29521 gctttttctt gacggatttg cgcttcgata cgcttggtat ccttgcggtt ttggctttga
    29581 gtggaagcgg ctggcacggt tgcggcgttt tcctgtgcca agcgccattg gcggtaatcg
    29641 ttcaaatcgc cgtcaaagtt tttcagacga cctttgtcta tcaagaggaa actgtcggtc
    29701 gtggcttcaa gcaggctgcg gtcgtgcgat acgacgatta atgcaccttg gaaactttgc
    29761 aacgcgagcg ttaaggcgtg gcgcatatcc aaatccagat ggttggtcgg ttcgtcaagc
    29821 agcagcaggt tcggcttttg ccagataatc atggcaaggg caaggcgggc tttttctccg
    29881 ccggaaaaag gttcggtttt ctgcaacgcc atatcgccga caaagttgaa gccgccgagg
    29941 aaatttcgga tttcttgttc gcgtacttcg ggagaaagct gctgtatatg ccaaacaggg
    30001 ctttggtcgg cgcggatggt gtcaagctgg tgttgggcga aatagccgat attaagtttt
    30061 tcggaatgga cgatgctgcc ggagagtaaa tcgatttttc ctgccaaagc tttgataaac
    30121 gtagatttac cgctgccgtt gacacccaat agcccgtaac gtgcgccgct ttccagcgac
    30181 agggtaatgt catgcaggac ggttttacct tcgtaaccca aatcagcgtg ctccagcttc
    30241 agcaaaggat tgggcagatg gtcgggattg taaaactcaa aggaaaactc gctgtccaga
    30301 tgtgcgggag cgatgcgttc gagcttcgcc aacgccttca tgcggctttg agcttgaacg
    30361 gctttagtgg ctttggcttt gaagcggtcg ataaaggatt gcagatgttt gatttgcgcc
    30421 tgctgtttga catatgcggc ttgttgttgc gccagacgct gcgcgcgttc ggtttggtaa
    30481 aaatcgtaat tgccgctgta ttgcgtgagc ttttgctgcg acaattcaat ggtttgggtg
    30541 gtggccgcat tgaggaaatc acggtcgtgg gaaatgatga tttgcgtgca gggtaaagag
    30601 gcaaggtggt tttccaacca caagacagtt tccaaatcca aatggttggt cggttcgtca
    30661 agcaagagca aatcagcgcg gcaaatcagg gcttgcgcaa gattcaggcg catacgccag
    30721 ccgccggaaa aggatttgac gggacggctg tgttcttctt gcgaaaaacc cagcccgttc
    30781 aacaattttg ccgcgcgggc cggcgcggta taggcgtcga tttcttccaa tttggcatga
    30841 tattccgcct gcttcatgcc gtcattttgc acttccgcct gcctcaatgc cgtctgaaaa
    30901 gcctgcaact cggcatcgcc ctgcaaaacg taatccaaag cggaaatatc caaatcgggc
    30961 gtttcttggg aaacggaagc gagccgccag tttttcggaa tcgagatatc gccgccgtcc
    31021 tgagtgattt cgcccttgat taaggcaaac aggctggatt tgcccgtccc gttcttaccg
    31081 atcaaaccga cgcgctgacc gggattgacg gcagcggaag ccttgtcgag caggactttc
    31141 aaaccgcgtt gcagggtgag gtttttgatt tcaatcataa cggaaacatc gtcgggcggg
    31201 aaaagcccgt attttacctg aaagtcagtg ccgatgccgt ctgaaacggg aaatttacgg
    31261 ttgaagccaa gcccaagccc tgcgcccttc cgagtgcagg aaaaccaatg tcctgaatgc
    31321 cgaatcggta ttcatgcatt ccacgctgat tccgattcgg gaaaaatccg ccatgatttt
    31381 gggatggata aactcctgag ccgcgcccgt cccgataatc aatatttccg gatagtcaac
    31441 aggtttgatg tcggacaaca ggttttccgg agtcaggtcg gacaaggttc ggcattgcgg
    31501 caggcagacc gaatccttat gtacaagcac gggtttatgg aaactttgcc ccgccagccg
    31561 gattccgccc gcgccgcatt catattctgc aaactgtccg tctatcggat tttcttcaaa
    31621 caacattttt taccccgttg ctgcatcatc tgcaccgaaa ggggtgcaaa atcaggcgaa
    31681 ttaatgtagg attggcagat ttcatctgac gcgcctgccg atttcagacg gcatttgatt
    31741 caaagtgcgg cacaattata ccggcagcgg atattttcgt ctttcaatat ttacatttca
    31801 gtcggcttac aaggagacac aatgaagcca gtaaacatcg gtcttttagg cttaggtacg
    31861 gtcggcggcg gtgcggctgc cgtgttgcgg gacaacgcag aggaaatttc ccgccgcttg
    31921 gggcgcgaaa tccgtatttc tgccatgtgc gacttaagcg aagaaaaagc ccgccaaatc
    31981 tgcccgtccg ccgcctttgt caaagatccg ttcgaactgg tcgcgcgtaa agacgtcgat
    32041 gtcgtcgtcg aattgttcgg cggcacgggc attgccaaag aagcggtgtt gaaagccatt
    32101 gaaaacggca aacacatcgt taccgccaac aaaaaactgc tcgccgaata cggcaacgaa
    32161 atcttcccgc tggcggaaaa acaaaacgtc atcgtccaat ttgaagcggc agtagcgggc
    32221 ggtatcccaa tcatcaaagc cctgcgcgaa ggtttggcgg caaacaggat taaatccatc
    32281 gccggcatta ttaacggcac cagcaacttc atcctctccg aaatgcgcga aaaaggcagc
    32341 gcgtttgccg acgtattgaa agaagcgcag gcattgggtt atgccgaagc cgacccgacc
    32401 ttcgacatcg aaggcaacga cgcaggccat aaaatcacca tcatgagcgc gctggcattc
    32461 ggtacgccga tgaacttctc cgcctgctat ctcgaaggca tcagcaaact cgacagccgc
    32521 gacatcaaat acgccgaaga actcggctac cgcatcaaac tgttgggcat cacccgcaaa
    32581 accggcaaag gcatcgaatt gcgcgtccat ccgaccctga ttcccgaaag ccgcctcttg
    32641 gcaaacgtcg acggcgtgat gaacgccgtg cgcgtcaacg ccaatatggt cggcgaaacc
    32701 ttatattacg gcgcgggcgc gggcgcattg ccgaccgctt ccgccgtggt tgccgatatc
    32761 atcgacatcg cccgcctggt tgaagccgac accgcccacc gcgtaccgca tctggcgttc
    32821 caacccgccc aagtccaagc gcaaaccatc ctgcctatgg acgaaattac cagcagctac
    32881 tacctgcgcg tccaagccaa agacgaaccg ggcacgctgg ggcaaatcgc cgcgctgttg
    32941 gcacaagaaa acgtgtccat cgaagcctta atccaaaaag gcgttatcga tcagaccact
    33001 gccgaaatcg tgattctgac ccacagcacg gtcgaaaaac acatcaagtc ggcaatcgca
    33061 gccatcgaag cactggattg tgtggaaaaa ccgattacca tgatccgcat ggaaagcctg
    33121 catgactgag ccgaaacacg aaacgccgac ggaagagcag gttgccgcgc gcaaaaaagc
    33181 aaaagccaaa atccgcacca tccgcatttg ggcgtgggtc attttggcgt tgctcgcttc
    33241 aaccgccctg ctctcccaat gcgcgatgtc caaaccgcag gcaaaacaga aaattgtcga
    33301 gtcttgcatg aaaaatattc cgtttgctga aaaatggcag aacgatttga aagcgcgcgg
    33361 cttggatgcg gacaataccc gtctcgccgt cgactactgc aaatgtatgt gggagcagcc
    33421 tttggacgga ttgagcgaga aacagatcag ctccttcggc aaactcggtg cacaagaaca
    33481 gcttgacctg ctcggcggcg caaacgcgtt tgaaactcga gacaaacaat gtgtcgcgga
    33541 tttgaaagcc gattgaacct caggtctgga atgccgtctg aacgcgggga aaagcgtttc
    33601 agatggcatt tttccatccg ggtttgacgg gcagcccttc atccgaatcc gacgggcaaa
    33661 cccctatcct gattcggcgg ggaaaacaaa aagccgattc ttcaaggaat cggcttttgc
    33721 tgaaaaacag tttacagtgc gtctttcaat gctttacctg cacggaattt aagcgttttg
    33781 gcggcggcaa tggtcagagg ctcgccggtt ttggggttgc ggccttggcg ttccgcacgt
    33841 tcgccgacgt agaaagtacc gaaaccgacc agagtaacgg tgtcgccttg tttcagggcg
    33901 ttggttactg cgttggtagt ggcatccaaa gctttttgtg cggcggcttt ggaaatatcg
    33961 gcttcttgag caatcgcttc gatcaattca gacttgttca caatcagtcc cttcctgtct
    34021 taaaaaatga tgaaatgccc gaatactcgg ggtttgtact gcttgagcaa ctttcgcttt
    34081 atagcaattc tgaaattgcc gtgtcaagca aaaaatacgg aatcgcccta ttttacaggc
    34141 tttcaggacg aaaccgcatt tttacaacac atttcctgcg tttcaatgtt tgattgccct
    34201 gctgcggggt ttcggttttg aagcggattc cgccgccgct tccgcggcag aaggttctgc
    34261 ccaagactca ggccggcttt ccaaacccaa agccaatacc tcgtctatcc atttgaccgg
    34321 atggatggtt aatccggttt tcacgttttc agggatttct tccaggtctt tgacattgtc
    34381 tttcggaatc aggacgtgtt tgatgccgcc gcgaagcgca gccaacagtt tttccttcag
    34441 accgccgatc ggcaggactt cgccgcgcag ggtaatttcg cccgtcatgg caacatcggc
    34501 acgcaccgga attttggtaa aggcggacac catcgccaaa gtcatagcaa tgcctgcgct
    34561 cggaccgtct ttcggcgttg caccttcggg aacatggaca tgaatgtctt ttttctcgta
    34621 aaaatcagga gccaaaccta ctgattccgc acgggaacgg acaacagacc atgcagcgga
    34681 tacagattcc ttcatcacat cgcccaactg gccggtacac tggatcatgc ctttacccgg
    34741 taatgctgcg gcttcgacag tcagcaattc gccgccgact tccgtccaag ccaaaccggt
    34801 aacctgtccg atgcggtttt cactttcggc aacgccgtaa tcgaagcggc gcacgcccaa
    34861 gtagtcatgt aggtttttct cattgacttt aaccgcccta ggcttggctt tgctggtttt
    34921 cttggtttca gacaacctct tcttatcttc gttcaaggta atctgcatca ccaccttacg
    34981 gcagattttg gcaatttcgc ggtcgagcga acgcacgccc gcctctcggg tgtaataacg
    35041 gataatatcg cgtaccgcgc tttcttcgac caccaattcg ccttctttca cgccgttgcg
    35101 tttcatttgc ttcggtacga gatactgcat cgcgatattg attttctcgt cttcggtata
    35161 gccggacaga cggatgattt ccatacggtc aagcagcggg gtcgggatat tcaggctgtt
    35221 tgaagtcgca ataaacatca cgtcgctcaa atcgtaatcc acttcggcat aatgatcggc
    35281 gaacttgttg ttttgttcgt gatcgagcac ttcaagcaac acgctggcgg gatcgccccg
    35341 aaagtcgctg cccaatttgt cgatttcgtc gagcaggaac aacgggtttc ttacgcctgc
    35401 ttttgccata ttctgcaaaa tcttgccggg catagaaccg atataggtac ggcggtgccc
    35461 gcggatttcg ctttcgtcgc gcacgccgcc caaggccata cggacatatt tccgccccgt
    35521 tgctttggcg atggattcgc ccaaagaggt tttgcccacg cccggagggc cgaccaaaca
    35581 cagaatcgga cctttgagct tgtccatacg tttttggacg gcgaggtatt ccaaaatccg
    35641 ttctttgact ttttccaggc cgtagtggtc ggcatccagc accagtccgg ctttggcgat
    35701 gtctttgctg acgcgggatt ttttcttcca cggcagtccg agcagggtgt cgatgtagtt
    35761 gcgtacgacg gtggattcgg cagacatcgg cggcatcatt ttgagttttt tcagttcgga
    35821 caggcatttt tcttccgctt ctttggtcat acccgccttt ttgatgcctg cctccaaggc
    35881 atccagttcg ccgttttcgt cttcttcgcc caattctttg tgtatcgctt taatctgttc
    35941 gttcagataa tattcgcgtt gggatttttc catttggcgt ttgacgcgtc cgcgtatgcg
    36001 tttttcggcc tgcataatgt cgagttcgga ttccaacttt gccagcagga attccatccg
    36061 tttgccgatt tcgggaatct ccaaaatctg ttggcgttgc gccagtttca actgcaaatg
    36121 cgctgcgacc gtatcggtta gccggctgtt ttcggcaatg ccgttgatgc tgccgataat
    36181 ttcggcaggg atttttttat tgagtttggc gtattgttca aactgcgcca acagggtgcg
    36241 gcgcacggct tcgaggtcgg tattgccgcc cgtgtcttct tccacgaccg cctctatatg
    36301 agaaacgaac agcccgcccg tgtcttcgat ggtcaaaaca cgtccgcgat acagcccttc
    36361 gaccagcact tttaccgtgc cgtcgggcag tttcaacact tgcaggactt gtgcgacggt
    36421 accggtctga tacaggtcgg cggcaaccgg ttcttctacc gccgcatcgg tttgcgccaa
    36481 caggaaaacc ggttcctcgc gggtaatggc gttttccagt gcggcgatgg atttcggcct
    36541 gccgacgaac agcggcagaa ccatatgcgg gtaaacgacg acatcccgta aaggaagggt
    36601 cgccaaagca gcatattcct caaaatgctt ttctttttgt gtcatggata ctctcttgtg
    36661 tctgacagga tacggattgc cgcgtaaatt ggggttgaaa gtattatttc aagcatacgc
    36721 ggtttattta tggagtttga tgccatgccg tctgaaacat tccggcttca gacggcatgg
    36781 gcttggaaag acaaggcggg aacaaaaaaa cagttctgtg ttgccgctcc ttgctgtacc
    36841 atccgtatgg tttgcggttc tgccgcccta ttttcaaaac atgacgcagg tatcccatgt
    36901 cttcttttat ttttcccgat aacgggttcg aatggcgcaa cgaaagtctg caaaagccgc
    36961 ccaaaggttg gtattatgtc cgcgaaagcg gtgcagacgg cattttgaag gctacctatc
    37021 aaaatattgc aactgtctgg cagggcgatt tccacaatgc caaacaggtg ctttcggcaa
    37081 tgaagaagaa ggtccgcaaa cctgccgccg tccgttccga tgcggatatc gccgcccttt
    37141 tccacgccca ccgtatgaag caggcgcaac agagccgtat tctgaatatg cttgccgttg
    37201 aaatccgccc cggtttcgtg ttggacaaca aacgcgcgcc cgatatacgc gccgctttgc
    37261 tcgacgtgta cggagaggcg gacggcaaac cgttttttct gccgctcaat ctgctgctgg
    37321 ggtttatggg tgtgcatgag tggcataag
//
LOCUS       DAXDXW010000011        37022 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXDXW010000011 DAXDXW010000000
VERSION     DAXDXW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37022
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.011"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            218..838
                     /locus_tag="ACFB64_000464"
     CDS             218..838
                     /locus_tag="ACFB64_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC1208889.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSKGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACLNLLIAASVWLRYRKDGYGEH"
     gene            861..2312
                     /locus_tag="ACFB64_000465"
     CDS             861..2312
                     /locus_tag="ACFB64_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HFC1208890.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIAGRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDIFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNSTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIRMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            2773..3129
                     /locus_tag="ACFB64_000466"
     CDS             2773..3129
                     /locus_tag="ACFB64_000466"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC1208891.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            3120..3602
                     /locus_tag="ACFB64_000467"
     CDS             3120..3602
                     /locus_tag="ACFB64_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC1208892.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            3615..4208
                     /locus_tag="ACFB64_000468"
     CDS             3615..4208
                     /locus_tag="ACFB64_000468"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC1208893.1"
                     /translation="MASIQNLYETVVGVLGDQAGKVISALGEITVECLPEHYISVMTA
                     LHDHEDLHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVADIYNSADWYEREAFDLYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            4198..5454
                     /gene="nuoD"
                     /locus_tag="ACFB64_000469"
     CDS             4198..5454
                     /gene="nuoD"
                     /locus_tag="ACFB64_000469"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC1208894.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPGFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCADWLRVNPGPVITANHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            5454..5927
                     /gene="nuoE"
                     /locus_tag="ACFB64_000470"
     CDS             5454..5927
                     /gene="nuoE"
                     /locus_tag="ACFB64_000470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC1208895.1"
                     /translation="MLSAKSLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            6317..7618
                     /gene="nuoF"
                     /locus_tag="ACFB64_000471"
     CDS             6317..7618
                     /gene="nuoF"
                     /locus_tag="ACFB64_000471"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC1208896.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVRRIVEGKGRMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFAHYIEHGGPMKEHKWGGW"
     gene            7618..7917
                     /locus_tag="ACFB64_000472"
     CDS             7618..7917
                     /locus_tag="ACFB64_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208897.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERYGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFILARQGHGVVVAMQ"
     gene            8331..10592
                     /gene="nuoG"
                     /locus_tag="ACFB64_000473"
     CDS             8331..10592
                     /gene="nuoG"
                     /locus_tag="ACFB64_000473"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFC1208898.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGVWANPMNTVEELYLAKKFAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAAHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANTPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWAGEGVQTASDRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMGTIELAGA"
     gene            10595..11671
                     /gene="nuoH"
                     /locus_tag="ACFB64_000474"
     CDS             10595..11671
                     /gene="nuoH"
                     /locus_tag="ACFB64_000474"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC1208899.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPRGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGIVGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            11752..12231
                     /gene="nuoI"
                     /locus_tag="ACFB64_000475"
     CDS             11752..12231
                     /gene="nuoI"
                     /locus_tag="ACFB64_000475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC1208900.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            12243..12914
                     /locus_tag="ACFB64_000476"
     CDS             12243..12914
                     /locus_tag="ACFB64_000476"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC1208901.1"
                     /translation="MTFSVILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKTE
                     GEGKA"
     gene            12911..13216
                     /gene="nuoK"
                     /locus_tag="ACFB64_000477"
     CDS             12911..13216
                     /gene="nuoK"
                     /locus_tag="ACFB64_000477"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC1208902.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            13219..15243
                     /gene="nuoL"
                     /locus_tag="ACFB64_000478"
     CDS             13219..15243
                     /gene="nuoL"
                     /locus_tag="ACFB64_000478"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC1208903.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPGATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAVKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLVVTLPLILLAIPSVIIGYIAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLATAGVLSAWL
                     LYIKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGNFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKVQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            15386..15889
                     /locus_tag="ACFB64_000479"
     CDS             15386..15889
                     /locus_tag="ACFB64_000479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208904.1"
                     /translation="MDKRLFDGTVRFEHVTGVGTMQVDFQPNQRVYTMIGENGVGKTK
                     FLECLFTVLLFTYRMANINIGFSWIDLQRLPLKIVELPNGKNEDFRRFSERSNQGFNI
                     NEDVLFSGYQHRLPFVYLPAQSRGWVKKQTSKLSRWEVLKGVQANIWGICFLFFNLAI
                     VHSRLNP"
     gene            15892..16554
                     /locus_tag="ACFB64_000480"
     CDS             15892..16554
                     /locus_tag="ACFB64_000480"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC1208905.1"
                     /translation="MENDLEEWIIQRARSANPYQANEDNRKIEIDTLLFLLNQFDGRI
                     APDFLEIKGDDRIFIKLDNQKRELSGLSSGFTSILKIMQSIVAGYSYFTNEQQIANVR
                     GVVLIDEIESHLHNEWQIKIVPLLKKLFPNTTFFITTHSSLVISQLEQGGAYRLVRSK
                     EDGVVYGKMIDYPSNASFVDLLNEAFGVDLNKKKIERAQERNQEQAKKPYWNWYVRNW
                     AD"
     gene            16565..16789
                     /locus_tag="ACFB64_000481"
     CDS             16565..16789
                     /locus_tag="ACFB64_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFC1208906.1"
                     /translation="MTVYLLDTNYLVYLADDDSDEEKRKAVLSDMAEKLQQDDNRFVI
                     TPLIRYEVLRGVDWGKSEKLSRLTGVLAQF"
     gene            16820..17026
                     /locus_tag="ACFB64_000482"
     CDS             16820..17026
                     /locus_tag="ACFB64_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208907.1"
                     /translation="MASNLYRFDKFEAERDNTPKNLEKRKFDMFHYATASVNNLEILS
                     HDTDVNKIKDLHERMRLEDSAELA"
     gene            17136..18632
                     /locus_tag="ACFB64_000483"
     CDS             17136..18632
                     /locus_tag="ACFB64_000483"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC1208908.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLTGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVVMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            18642..20087
                     /gene="nuoN"
                     /locus_tag="ACFB64_000484"
     CDS             18642..20087
                     /gene="nuoN"
                     /locus_tag="ACFB64_000484"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC1208909.1"
                     /translation="MNWSDLNLMPALPEVALLSLLVLLLPADLWASDDKCRWTHYGAL
                     ATVAVTAAVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFVLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWMPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHTGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLVGAFYYLRVVKVMYFDESGRARPAAGGNNAAKS
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            20199..20498
                     /locus_tag="ACFB64_000485"
     CDS             20199..20498
                     /locus_tag="ACFB64_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC1208910.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTASLAYILESRAGAVHNQGWEFYATVVCLYLIFAFPCFVRRYFWHTRNRE"
     gene            complement(20615..21511)
                     /locus_tag="ACFB64_000486"
     CDS             complement(20615..21511)
                     /locus_tag="ACFB64_000486"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC1208911.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSGNEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGAVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLERMHSLKTGALIRAAVLLGATACPDLSDAELAVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(21495..21719)
                     /locus_tag="ACFB64_000487"
     CDS             complement(21495..21719)
                     /locus_tag="ACFB64_000487"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC1208912.1"
                     /translation="MKKNTPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGTKELNLESDE"
     gene            complement(21832..22755)
                     /gene="rsgA"
                     /locus_tag="ACFB64_000488"
     CDS             complement(21832..22755)
                     /gene="rsgA"
                     /locus_tag="ACFB64_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC1208913.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITAGYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWLEKLKFYETLGYPVI
                     ETRVLENADSLRPVLQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPHYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETGAASPERLAFLQGITDELLG"
     gene            complement(22760..24616)
                     /locus_tag="ACFB64_000489"
     CDS             complement(22760..24616)
                     /locus_tag="ACFB64_000489"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC1208914.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLCRFVWSSMDGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMAFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIIGFASVMRLLIPRLGQTAARQAN
                     ARSLMTGRITDAYSNIATVKLFFHGAREAVYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSAAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGI
                     VNDGMATLSKPHTILDKPQALPLNVPQGAIKFEHVDFCYEAGKPLLNGFNLNIKPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRSGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMEGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLSEHVEWQHD"
     gene            complement(24642..24779)
                     /locus_tag="ACFB64_000490"
     CDS             complement(24642..24779)
                     /locus_tag="ACFB64_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208915.1"
                     /translation="MPSEGGAAFALTKPCVSAPRCLPDGFSRKMALLSLLSDGIVFDA
                     V"
     gene            complement(24779..25363)
                     /gene="ruvA"
                     /locus_tag="ACFB64_000491"
     CDS             complement(24779..25363)
                     /gene="ruvA"
                     /locus_tag="ACFB64_000491"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC1208916.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAASPAADETEDIVSTLLA
                     LGYNEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            complement(25445..25771)
                     /locus_tag="ACFB64_000492"
     CDS             complement(25445..25771)
                     /locus_tag="ACFB64_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC1208917.1"
                     /translation="MNIKHLLLTAAATALLGISAPALAHHDGHGDDDHGHAAHQHGKQ
                     DKIISRAQAEKAAWARVGGKITDIDLEHDDGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(25832..26518)
                     /locus_tag="ACFB64_000493"
     CDS             complement(25832..26518)
                     /locus_tag="ACFB64_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC1208918.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKPEKLHASANRSYKV
                     AEFTQTGNASWYGGRFHGRKTSGGDRYDMNAFTAAHKTLPIPSHVRVTNTKNGKSVIV
                     RVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSAPVAENKDIFIDLK
                     SFGTEHEAQAYLNQAAQNFAASSSSPNLSVEKRRYEYVVKMGPFASQERAAEAEAQAR
                     GMVRAVLTSG"
     gene            complement(26646..27110)
                     /gene="trmL"
                     /locus_tag="ACFB64_000494"
     CDS             complement(26646..27110)
                     /gene="trmL"
                     /locus_tag="ACFB64_000494"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC1208919.1"
                     /translation="MLTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKSLAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMRPGSRSMNLSNTVSVILFEAWRQHGYAGGV"
     gene            complement(27175..27900)
                     /locus_tag="ACFB64_000495"
     CDS             complement(27175..27900)
                     /locus_tag="ACFB64_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC1208920.1"
                     /translation="MNFLSCWRRIADAPTIRRCVLCHGSSGVSDGICAGCNTDLASFR
                     TDAANSCPLCFRHVQGGAVCGGCQKKPPAFDRMWASLHYEPPVSNMIRALKHLADLGM
                     AQPLADLTMQNPPDRLSDECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQIL
                     PRHTVFRHHRPPQSTLKGGERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELA
                     KTLKKSGANRICCWTLARTPMKK"
     gene            27899..28648
                     /gene="bioH"
                     /locus_tag="ACFB64_000496"
     CDS             27899..28648
                     /gene="bioH"
                     /locus_tag="ACFB64_000496"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFC1208921.1"
                     /translation="MPDAAKKVYLIHGWGANRHAFDDLMPRLPATWPVSAVDLPGHGD
                     APFAQPFDIEAAADGIAAQIDTSADILGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTAAEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTPDAAEIIGRILPDLAR
                     CGTPQALQEALDAAERADVRHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRL
                     VVMEKAAHAPFLSHAEVFAALYRDFVEGGLT"
     gene            28645..29433
                     /locus_tag="ACFB64_000497"
     CDS             28645..29433
                     /locus_tag="ACFB64_000497"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC1208922.1"
                     /translation="MNPQDKCWQVHRHLAEHTDQRLTLVRNAPGHILLAGADADISRS
                     LLAKRYPLAVFEEYDSRADFLAAAAAARKGGFWQKLTGRGVVQHCQSPTVPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTLAELKSRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAVAWDDENAA
                     RSCVGAIFEREGGLGITLETVYGHAVKKLVLPQGENVVRFFPKR"
     gene            complement(29514..29621)
                     /locus_tag="ACFB64_000498"
                     /pseudo
     CDS             complement(29514..29621)
                     /locus_tag="ACFB64_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            29743..31116
                     /locus_tag="ACFB64_000499"
     CDS             29743..31116
                     /locus_tag="ACFB64_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HFC1208923.1"
                     /translation="MDWRGNKPLGAAELADLKPLYKDFMYWERGLHMYKASAVVPTGY
                     VRVGNTAPLCGEDTQRYASFWGDGYDVYRQLRWRQIPEKQRKAFKKAAKSKNTVMFAG
                     REYGISKQNLSDVWDDFEDAMELKAFPCLSSLFLTKWHKNLYEYLEEYPFITRLCLEN
                     HGQTVLDFSNTRITDLSVDMTGVESLYLNEGLDSLNLKGEIKENCKVCTAGKGAGLIL
                     EVGKSVPKVRGLENLTAVNVMGIADFDMQNLSETYPKLKTIRLWGKPGNIANFSAVSG
                     FEDLEVFTAVDLFGFGADDIPHPDRLPKLHRLWMSSLPEEAAKAVKKLYKKRKEDGLD
                     LWIEKARKPEWLAQNFDNPFRDWDGAEHIPKSHAKKAAELYRKTRAGVVKLLGNPPEN
                     TGEGLAEAVKAYTGGFNKMDKKHFIDTVEREDIAEALETILDLIPDGSCADKEKLFEI
                     FDKNRNF"
     gene            complement(31153..33456)
                     /gene="recQ"
                     /locus_tag="ACFB64_000500"
     CDS             complement(31153..33456)
                     /gene="recQ"
                     /locus_tag="ACFB64_000500"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC1208924.1"
                     /translation="MTHRPTARQILHEVFGYPEFRGRQEDVINTLAGGGSLTVLMPTG
                     GGKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIA
                     DKLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSRWGHDFRPEYQQLG
                     MLAERYPNIPRIALTATADAATRADIKHYLHLDDASEFVSSFDRTNIYYQVIEKNNGK
                     KQLLDFIRKEMTGQSGIVYCLSRKKVEDAAQFLRENGLNAIPYHAGLSMDVREENQRR
                     FTHEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLC
                     YGLNDWVLLRERIAEGNSGEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEASEPC
                     GHCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQL
                     STFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKVLKGETEVMLRPL
                     KRDKPATRTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKM
                     PQNLNGLHDIYGLGEAKTERFGHGILKICQNAADFSHDAVIRPQTEREQQLRQKLEAW
                     RYEQARAENCALHTVLSDESLADMLAATPETETDLEAVHGLGSVRAAKYGRDILAVCR
                     PFSDGIDETAKHKRCLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAEL
                     AAVYGVGEEKAARYGAAVLAVLERDAV"
     gene            complement(33519..34301)
                     /gene="trpC"
                     /locus_tag="ACFB64_000501"
     CDS             complement(33519..34301)
                     /gene="trpC"
                     /locus_tag="ACFB64_000501"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC1208925.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVLLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQGQLERFEALA
                     HELGMTVLLELHDETELEKCRNLTTPLRGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIGAEVGKLF"
     gene            complement(34352..35308)
                     /locus_tag="ACFB64_000502"
     CDS             complement(34352..35308)
                     /locus_tag="ACFB64_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFC1208926.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQHPAPAYRREMLPDSTGKT
                     KTAYDFSGGISPDAPLVVLFHGLEGSSRSHYAAELMLAVRNRGWHGAVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFALDTLAARYREIYAVGVSLGGNAPAKYLGEQGKKALPHASA
                     AVSAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPRADEASEAV
                     TLFQPAHGGHAGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRH"
     gene            complement(35441..36979)
                     /gene="murJ"
                     /locus_tag="ACFB64_000503"
     CDS             complement(35441..36979)
                     /gene="murJ"
                     /locus_tag="ACFB64_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC1208927.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLIVVTALG
                     ILAAPWVIYVSAPGFTKDADKFQLSISLLRITFPYILLISLSSFVGSILNSYHKFGIP
                     AFTPTFLNISFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAAGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLASGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRKHGIYRPGRGWAAFLAKMLL
                     ALAVMCGGLWAAQACLPFEWAHAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
BASE COUNT         8023 a         9482 c        10475 g         9042 t
ORIGIN      
        1 aaaatcgagt ttatcctagt tgtccaggac agccccttaa tttttataac gtcatgaagc
       61 cgcacagcag tttgaacagt gatacgccgt ttgcgggctt acgagtttat tttcccggcc
      121 tgcagtttga gcaatgcggt gatttcctac ggctaataca aatgtttaca cattaataca
      181 tttcatttat agttccgcct atttgaaaat agaaaatatg aattcgaccg caagtaaaac
      241 cctgaaagga ttgtcgctgg tgtttttcgc ctctggcttc tgcgccctga tttaccaggt
      301 cagctggcag aggcttctat tcagccacat aggtatcgat ttgagttcga ttactgtcat
      361 tatttctgta tttatggtcg gcttgggtgt aggtgcgtat ttcggcggac gcattgctga
      421 ccgttttcct tcaagtatca tccccctgtt ttgcatcgct gaagtatcca tcggtctgtt
      481 cggtttggta agcaagggtc tgatttccgg cttggggcat cttttagttg aggctgattt
      541 gcccatcatc gctgctgcca atttcctctt attgctgctt cctaccttta tgatgggcgc
      601 gaccttgccc ttgctgacct gtttttttaa ccggaaaata cataatgttg gcgagtctat
      661 cggtacctta tattttttca acactttggg tgcggcactc ggatcgcttg ccgccgccga
      721 atttttctac gtctttttta ccctctccca aaccattgcg ctgacagcct gccttaacct
      781 tctgattgct gcttcagtat ggctgcgtta cagaaaggat ggatatggtg aacactaaac
      841 cgaatactag tttgatttat atgctttctt tccttagcgg attattgagc ttgggtatag
      901 aagtcttgtg ggtaaggatg ttttcgttcg cagcacagtc cgtgcctcag gcattttcat
      961 ttactcttgc ctgttttctg accggtatcg ccgtcggcgc gtattttggc aaacggattt
     1021 gccgcagccg ctttgttgat attcccttta tcgggcagtg cttcttgtgg gcgggtattg
     1081 ccgatttttt gattttgggt gctgcgtggt tgttgacggg tttttccggc ttcgtccacc
     1141 acgccggtat cttcattacc ctgtctgccg tcgtcagggg gttgattttc ccacttgtac
     1201 accatgtggg tacggatggc aacaaatccg gacgacaggt ttccaatgtt tatttcgcca
     1261 acgttgccgg cagtgcattg ggtccggtcc ttatcggctt tgtgatactt gatttcttgt
     1321 ccacccaaca gatttacctg ctcatctgtt tgatttctgc tgctgtccct ttgttttgta
     1381 cactgttcca aaaaagtctc cgactgaatg cagtgtcggt agcagtttcc ctaatgttcg
     1441 gcatcctcat gttcctactg ccggattctg tctttcaaaa tattgctggc cgtccggata
     1501 ggttgattga aaacaaacac ggcattgttg cggtttacca tagagatggt gataaggttg
     1561 tttatggggc gaatgtatac gacggcgcat acaataccga tatattcaat agtgtcaacg
     1621 gcatcgaacg tgcctatctg ctaccctccc tgaagtccgg catacgccgc attttcgtcg
     1681 ttggattgag tacaggttcg tgggcgcgcg tcttgtctgc cattccggaa atgcagtcga
     1741 tgatcgttgc ggaaatcaat ccggcatacc gtagccttat cgcggacgag ccgcaaatcg
     1801 caccgctttt gcaggacaaa cgtgttgaaa ttgtattgga tgacggtagg aaatggctgc
     1861 gtcgccatcc tgatgaaaaa ttcgacctga ttttgatgaa ttcgacttgg tactggcgtg
     1921 cctattccac taacctgttg agtgcggaat ttttaaaaca ggtgcaaagc caccttaccc
     1981 cggatggtat tgtaatgttt aataccacgc acagcccgca tgcttttgct accgccgtac
     2041 acagtattcc ctatgcatac cgctacgggc atatggtagt cggctcggca accccggtag
     2101 ttttccctaa taaagaactg ctcaagcaac gcctttcccg gttgatttgg ccggaaagcg
     2161 gcaggcacgt atttgacagc agcaccgtgg atgctgcagc acaaaaggtt gtctctcgta
     2221 tgctgattcg gatgacggaa ccttcggctg gggcggaagt cattactgac gataatatga
     2281 ttgtagaata caaatacggc agagggattt aaccgtctta aagggtttca ggcaacgcag
     2341 gttttaggta acgtcctgct agttcaaaaa aaccgcatca cagcagtcgg gacaaaatgg
     2401 tttaaacatt ttgtcccgaa ttcttattcc tatatatagc ggattaacaa aaatcaggac
     2461 aaggcggcgg gccgcaggca gtacaaatag tacggaaccg gttcgcttgg tgcttgggca
     2521 ccttagggaa ccgttcccct tgagctaagg cggggcaacg ccgtaccggt ttttgttaat
     2581 ccgctatacc acgaattacg gtgtaaaaat ttatatgacc ttataaaatc aaataagaat
     2641 cgttatcata acatgattgt atttattggg tttttttggg cgttttgccg atatttacct
     2701 tttaatggtt tttgaaattc gctaaaatac gaaattattg tagaaatttt gttaacggat
     2761 ttgggtgtaa ccatgttgtc cgcttacttt cccgtctttg tctttatcct cgtcggcctc
     2821 gcggccggcg tactgtttat cctgctcggc acgattttgg gcccgaaacg ccactatgcc
     2881 gaaaaagacg cgccttacga atgcggtttt gaagcctttg aaaacgcaag gatgaagttc
     2941 gacgtgcgct attacctcgt cgccatcctg ttcatcctct tcgatttgga ggttgcgttt
     3001 atgctgccgt gggcagtcgt gttcaaagat ttgggcgcgt acggcttttg gtctatgctg
     3061 gtgtttatcg tcgtcctgac ggtaggcttt gtttacgaat ggaaaaaagg tgcgctggaa
     3121 tgggaataga aggcgttttg aaaaaaggtt tcatcaccac cagcgcggat acggtgttga
     3181 actatatgcg taccggttca ttgtggccgg ttactttcgg attggcctgc tgcgccgtgg
     3241 aaatgatgca tgcgggtatg gcgcgttatg accttgaccg tttcggtatc attttccgtc
     3301 cgtccccacg tcaggccgac ctgatgattg tggcaggtac gctgaccaat aaaatggcgc
     3361 cagccctgcg tcgcgtgtac gaccagctcg ccgagccgcg ctgggtattg tctatgggtt
     3421 cgtgtgccaa cggcggcggc tattatcact attcttattc cgttgtgcgc ggtgccgacc
     3481 gtgtcgtgcc ggtggacgtt tatgtgccgg gttgtccgcc gactgcggaa gctctgattt
     3541 acggcctgat tcagctccaa caaaaaatca agcgcacttc caccatcgcg cgtgacgagt
     3601 aaggagagga cgatatggca agcattcaaa acttatacga gaccgtcgtc ggcgtgcttg
     3661 gcgatcaggc aggcaaagtc atttcagctt tgggcgagat taccgtcgag tgtctgcccg
     3721 agcactatat ttcagtcatg accgcattgc acgaccatga agatttgcat ttcgagcttc
     3781 tggttgactt gtgcggcgtc gattacagca cttacaaaaa cgaagcatgg cagggcaaac
     3841 gctttgccgt tgtcagtcag ttgctttccg ttaaaaacaa tcaacgcatc cgcgtacgcg
     3901 tctgggtttc agacgacgac ttccctgtag tcgaatccgt agccgatatt tacaacagcg
     3961 cggattggta cgagcgcgaa gccttcgatt tgtacggcat catgttcaac aaccatcccg
     4021 acttgcgccg catcctgacc gattacggct ttgtcggaca tccgttccgc aaagacttcc
     4081 cgatttccgg ctatgtggaa atgcgttatg acgaagagca aaaacgcgtg atttaccaac
     4141 ctgttaccat tgagccgcgc gaaatcacac cgcgtatcgt ccgtgaggag aactacggtg
     4201 gccaataaat taagaaacta caccatcaac ttcggcccgc agcaccctgc ggcgcatggc
     4261 gtattgcgta tgattttgga gctggacggc gaacaaatcg tccgtgccga tccgcatatc
     4321 ggcctcctgc accggggcac cgaaaaactg gcggaaacca aaacctatct gcaagccctg
     4381 ccctatatgg accgtttgga ctacgtttcc atgatggtca acgagcaggc gtattgcttg
     4441 gcggtagaaa aacttgccgg tatcgatgtg cctatccgcg cccaatacat ccgcgtgatg
     4501 ttcgccgaag taacgcgcat cctcaatcac ttgatgggca tcggttcgca cgccttcgac
     4561 atcggcgcga tgaccgccat cctctacgcc ttccgcgacc gcgaagagct gatggacttg
     4621 tacgaagccg tgtccggcgc acgtatgcac gccgcctact tccgtcccgg cggcgtttac
     4681 cgcgacctac ccggctttat gcccaaatac gagagcagca aattccgcaa tgccaaagta
     4741 ttgaagcagc tcaacgaatc ccgcgaaggc acgatgcttg actttatcga tgccttctgc
     4801 gaacgcttcc ccaaaaatat cgacacactc gaaaccctcc tgaccgacaa ccgcatctgg
     4861 aaacagcgta ccgtcggcat cggcgttgtc tctcccgagc gcgccatgca aaaaggcttt
     4921 accggtgtga tgcttcgcgg ttccggcgtg gaatgggacg tgcgtaagac acagccctac
     4981 gaagtgtacg acaaaatgga tttcgacatc cccgtcggcg tcaacggcga ctgctacgac
     5041 cgttacctct gccgtatgga agaaatgcgc caatccgtac gcatcatcaa acaatgcgcc
     5101 gactggttgc gcgtcaatcc cggcccggtc attaccgcaa accacaaatt cgctccgccc
     5161 aaacgtaccg aaatgaaaac aggtatggaa gacctgattc accatttcaa actctttacc
     5221 gagggtatgc acgttcccga gggcgagacc tacaccgctg tcgaacaccc gaaaggcgag
     5281 ttcggcgttt acatcatttc agacggcgca aacaaaccct accgcctgaa aatccgcgca
     5341 cccggcttcg cccacctgca aggcatggac gaaatggcaa aaggccatat gctcgccgac
     5401 gtcgttgcca tcatcggtac gcaggacatc gtattcgggg aggttgaccg ataatgttat
     5461 ccgcaaaatc cttaaaacaa atcgacattg agttggcgaa atatcccgcc gaccaacgcc
     5521 gttccgccat catgggcgcg ttgcgtatcg cccaaaccga aaaaggctgg ctcgcccccg
     5581 aaaccatcgc ttttgttgcc gactacatcg gcatcacgcc ggcgcaggca tatgaagtcg
     5641 ccactttcta caatatgtac gaccttgagc ctgtcggcaa atacaaactg accgtttgta
     5701 ccaacctgcc ctgcgctctg cgcggcggta tggcgaccgg cgaatacctc aaacaaaaac
     5761 tcggtatcgg ctacggcgaa accacgcccg acggcaaatt tacccttgtc gaaggcgaat
     5821 gcatgggcgc gtgcggcgat gcccccgtta tgctggtcaa caaccacagc atgtgcagct
     5881 ttatgaccga agaggcgatt gagaagaaac tggcggagtt ggagtaggtc gtctgaaacg
     5941 acgatttaaa cgtaggtcgg atacttgtat ccgacagagt gggtaaaaag gcaaaatgtc
     6001 ggatttaaga atccgcccta ctgaaatact gaaatgccgt cattcccgcg caggcgggaa
     6061 tccaccggta agattcggtt tctgaattta ataagacatt gcttaccatt gaggatggat
     6121 tcccgcctgc gcgggaatga cggcagacaa gcaagtggtc gagatccaac aaaaacgatt
     6181 gaaggtcgtc tgaaaatatc gatttgataa actagatttt atttcagaag acgttacaag
     6241 ccgatacaca aagacatttt aaggtcgtct gaaacagcgg ccgcaaccga tacgaaaaca
     6301 aacaggcaca ccaaaaatgg ctatttacca atcaggcgtg atttttgacc aagtggatac
     6361 cgccaatccc gattgctgga cattggacga atacgtcaaa cgcggcggct ataccgccct
     6421 gcgtaaaatc ctgtccgaaa acatctcgca aaccgatgtg attgacgaag tcaaaacctc
     6481 cggcttgcgc gggcgcggcg gtgcgggctt cccgaccggt ttgaaatgga gctttatgcc
     6541 ccgttctttc ccgggcgaaa aatatgtggt ttgcaacacc gacgaaggcg agccgggtac
     6601 atttaaagac cgcgacatca tcatgttcaa tcctcatgcc ctgattgaag gcatgatcat
     6661 tgccggttac gcgatgggcg cgaaagccgg ctacaactat atccacggcg aaattttcga
     6721 aggttatcaa cgctttgaag ccgctttgga gcaggcgcgt gccgcaggct ttttgggtaa
     6781 aaatattttg ggttcggatt ttgaattcga actcttcgcc caccacggct acggcgcata
     6841 tatttgcggc gaagaaaccg cattgcttga atcgttggaa ggcaaaaaag gccagccgcg
     6901 cttcaaaccg ccgttccccg cctcgttcgg cctgtacggc aaaccgacca ccatcaacaa
     6961 taccgaaacg ttctcctccg ttccattcat tatccgtgac ggcggacagg cgtttgccga
     7021 taaaggtatt ccgaatgcag gcggtaccaa attattttgt atttccggcc atgtcgagcg
     7081 tccgggcaac tatgaagtgc cgttgggcac gccgtttgcc gaagtcttga aaatggcggg
     7141 cggtatgcgc ggcggtaaaa aactcaaagc cgttattccc ggcggttcgt ccgcccccgt
     7201 attgcctgcc gacattatga tgcagaccaa tatggactat gactcgattt ccaaagccgg
     7261 ctcgatgctc ggttccggcg cgattatcgt gatggacgaa gacgtgtgca tggtcaaagc
     7321 cctcgaacgc ttaagctatt tctactacga cgaatcctgc ggccaatgca ccccctgccg
     7381 cgaaggtacc ggctggcttt accgcatcgt ccgccgcatc gtagaaggca aaggccgcat
     7441 ggaagacttg gatttgctgg attctgtcgg caaccaaatg gcaggccgca ccatctgcgc
     7501 ccttgccgat gccgccgttt tccctgtccg cagctttacc aagcatttcc gtgatgagtt
     7561 tgcgcattac atcgaacacg gcgggccgat gaaagagcat aagtggggag ggtggtaatg
     7621 gtggaagcta aaatttttat tctatacggt gcagccaaca aaggtaagag tacgacactc
     7681 aatacgcttt ttaatcagat ttgtcggaaa ttttctaaat ttctagtctt ttttgaaaga
     7741 tatggaaacg gcttagattt tgttgcagta tttgatcatg aaggtcagag gattggtttt
     7801 tattcatctg gtgataatga atacgaggtt aggagaaatt tatacaaact ttattcgcat
     7861 aattgtgatt ttattttggc acgtcaagga cacggggtgg tagttgcgat gcagtaggat
     7921 attgcatggc gatgtaaata taattaattg gtgtgaaaag tttgagccta cagatgaaga
     7981 caatgagcgt gctgttaaag agttatttaa gtcatttaaa aatataataa atgagttata
     8041 gttttagttg gttttatatt ggttaaaagc aaaatgctaa aaatttaact ttgccgtcat
     8101 tcccgcacag gcgggaatcc atagtggaat ttacagaacc cgatatttga aaagcagttg
     8161 ccgaaattca aaaaatggat tcccgcctgt gcgggaatga cggcaaggtg gcagtaaacg
     8221 ttttaaacag tattgattgt caatgaaact caaaaggccg tctgaagccc atttttcaga
     8281 cgatctccat aaaaaattat ttatcaaata cccgtaacta ggaacgaacc atgttacaaa
     8341 tcgaaatcga cggcaaacag gtatctgtgg agcagggcgc gacggtaatt gaagccgcgc
     8401 acaagctcgg tacttatatt ccgcatttct gttaccacaa aaagctctct attgccgcca
     8461 actgccgtat gtgtctggtg gatgtggaaa aagcccccaa accgctgcct gcctgtgcca
     8521 cgccggtaac ggacggcatg attgtgcgta cgcattcggc aaaagcccga gaggcgcaag
     8581 aaggcgtgat ggagttcctg ctcatcaacc atccgcttga ttgtccgacc tgcgaccagg
     8641 gcggcgaatg ccagttgcag gatttggcgg tgggctacgg caaaaccacc agccgttata
     8701 ccgaagaaaa acgttccgtc gtcggcaagg acatgggtcc tttggtttcc gccgaggaaa
     8761 tgagccgctg tatccactgt acccgctgcg tacgtttcac cgaagaaatc gccggtttgc
     8821 aggaaattgc gatggtgaat cgtggtgaac actccgaaat catgcccttt atcggcaaag
     8881 cggtggaaac cgagctgtcg ggcaacgtca tcgatttgtg tcccgtcggc gcattgacca
     8941 gcaaaccgtt ccgcttcaac gcgcgtactt gggaattgaa ccgccgcaaa tccgtttccg
     9001 cacacgacgc tttgggcagc aacctgattg tacaaaccaa agaccatacc gtccgccgcg
     9061 tgttgccgtt ggaaaacgaa gccatcaacg aatgctggct gtccgaccgc gaccgttttg
     9121 cctacgaagg cctgtatcac gaaagccgtc tgaaaaatcc gaaaatcaaa cagggtggcg
     9181 agtggatgga cgtggattgg aaaaccgcgt tggaatatgt ccgcagcgcg attgaatgta
     9241 tcgccaaaga cggcaaccaa aaccaagtcg gtgtttgggc caatccgatg aatacggttg
     9301 aagagctgta tctggccaaa aaatttgccg acggcttggg tgttaaaaac tttgcaaccc
     9361 gtttgcgcca acaagacaaa cgtctttcag acggccttaa aggtgcgcaa tggttgggac
     9421 aaagtattga atctttggct gacaacgatg ccgtattggt agtcggtgcg aacttgcgca
     9481 aagaacagcc gctcctgacc gcccgcctgc gccgcgccgc caaagaccgt atggccttga
     9541 gcgtattggc cggcagtaaa gaagaattgt ttatgccgct tctgtctcaa gaagccgcac
     9601 atcccgacga gtgggcaggc cgtctgaaaa acctgtctgc cgatgcggaa catgccgtta
     9661 ccgccagcct gaaaaacgct gaaaaagcag cggtgatttt gggtgcggaa gtgcaaaacc
     9721 atcctgatta cgccgccatt tatgccgccg cgcaagagtt ggcggacgcg accggcgcag
     9781 tgctgggcat tttgccgcaa gccgccaaca gcgtcggtgc ggacgtattg ggtgtgaact
     9841 cgggcgagag cgttgccgaa atggcaaaca cgccgaaaca ggcagtcttg ctgctcaacg
     9901 tcgagcctga aatcgatacg gttgacggtg caaaagccgt agccgcgttg aaacaggcga
     9961 aaagcgtgat ggcgtttacg ccgtttgtca gcgaaacgct gctggacgtg tgcgacgtat
    10021 tgctgccgat tgcgccgttt accgaaacat caggcagctt catcaatatg gaaggccgtc
    10081 tgcaatcttt ccacggcgtg gtacaaggct tcggcgattc acgtccgttg tggaaagtgt
    10141 tgcgcgtatt gggcaacctg tttgacctga aaggttttga ataccacgat accgccgcga
    10201 ttctgaaaga cgcgctggat gcggaaagcc tgccgtccaa actggacaac cgcagcacat
    10261 gggcagggga gggcgttcag acggcctcag accgcctcgt ccgtgtcggc ggcgtcggta
    10321 tttatcatac tgattctatc gtgcgccgtt ccgcaccgtt gcaagaaacc agccatgccg
    10381 ccgtgcctgc cgcgcgtgta aatccgaaca cattggcacg cttgggcctg caagacggac
    10441 aaaccgctgt cgccaaacaa aacggcgcaa gcgtatcggt tgccgtcaaa gccgatgccg
    10501 gtttgcctga aaacgtggtg catctgccgc tgcataccga aaatgccgcg ctgggtgcgt
    10561 tgatgggcac tattgaactg gcgggagctt gattatgcag gaatggttcc aaaacctctt
    10621 tgccgcaacg ctcggtctgg gcgatttggg catcaccgta ggcttggtgg tatccgtcat
    10681 cgtcaaaatc gtgattatcc tgattccgct gattctgacc gttgcctacc tgacgtattt
    10741 cgaacgtaaa gtcatcggct ttatgcagct tcgcgtcggc ccgaacgtaa ccggcccgcg
    10801 gggtctgatt cagccgtttg ccgacgtgtt caaactcttg tttaaagagg taacccgtcc
    10861 gaagctgtca aacaaagcct tattctacat cggcccgatt atgtcgctcg ccccgtcttt
    10921 cgcggcgtgg gcggtgattc cgtttaacga agaatgggtg ctgaccaata tcaatatcgg
    10981 ccttttgtac atcctgatga ttacctcgct gtcggtttac ggcgtgatta tcgcgggctg
    11041 ggcttccaac tccaaatatt cgttcttggg tgcaatgcgt gcttccgcgc aaagcatttc
    11101 ctacgagatt gccatgagtg ccgcgctggt gtgcgttgtg atggtatcgg gcagcatgaa
    11161 cttctccgac atcgttgccg cgcaggcaaa aggcatcgcg ggcggttctg tgttctcgtg
    11221 gaactggctg ccgctgttcc caatcttcat cgtctatctg atttccgccg ttgccgaaac
    11281 caaccgcgca ccgtttgacg tggcagaggg cgagtctgaa atcgttgccg gacaccacgt
    11341 cgaatactcc ggctttgcat tcgcgctgtt cttccttgcc gaatacattt tcatgattct
    11401 gattgccgcg ctgacatcgc tgatgttcct cggcggctgg ttgtctccgt tcccgcaaag
    11461 ctggggcatt gtcggtacgc cttccgcatt ctggatgttc gtgaaaatgg cggcggtgct
    11521 gtactggtat ctgtggattc gtgcaacctt cccacgctac cgctacgacc aaatcatgcg
    11581 tttgggctgg aaagtgctga ttccgatcgg cttcgcctac atcgtggttt tgggcgtgtg
    11641 gatgatttca ccgctgaatt tgtggaaata agtttcagac ggcatcttga ggccgtctga
    11701 acaaagcgat tttgaatacc taacgaaatc cctgttttga gggaacataa tatggctaac
    11761 ttagtaaaaa cctttctgct tggcgaactg gtcaaaggca tgggcgtaac gctcaaaaac
    11821 ttttttgccc gcaaagacac aatttatttt cccgaagaga aaacgccgca atccgtgcgt
    11881 ttccgcggtc tgcacgcgca acgccgttat ccgaacggag aggaacgctg catcgcgtgt
    11941 aaattgtgcg aggcggtgtg tccggcaatg gcgatcaaca tcgaatcgga agaacgcgaa
    12001 gacggcaccc gccgcaccaa gcgttacgac atcgacctga ccaagtgcat cttctgcggt
    12061 ttctgcgaag aggcctgccc gaccgatgcg attgtggaaa cccatatttt tgaataccac
    12121 ggcgagaaaa aaggcgactt gcatatgacc aagcccatcc tcttggcaat cggcgacaaa
    12181 tacgaagccg aaatcgccaa acgcaaagcc gctgacgcgc cgtaccgcta aggagcagac
    12241 gaatgacttt ttccgtgatt ctgttctata tccttgccgc catcgttttg tacggtgcgg
    12301 ttcgtaccgt taccgccaaa aaccctgtcc atgccgcttt gcatctggtg ctgaccttct
    12361 gcgtgagcgc gatgatttgg atgctgatgc aggcggagtt tttgggcgtt acgctggtgg
    12421 tggtttacgt cggcgccgta atggtgttgt tcctgttcgt cgtgatgatg ctgaatatcg
    12481 acatcgagga aatgcgcgcc ggtttctggc ggcacgcgcc tgttgccggt gtggtcggta
    12541 cgttgttggc ggttgccctg atcctgattc tggtcaaccc gaaaaccgac ctggccgcat
    12601 ttggtctgat gaaagacatt ccggccgatt acaacaatat ccgcgatttg ggcagccgta
    12661 tttataccga ctacctgttg ccgtttgaat tggcggcggt attgctgctg ttgggcatgg
    12721 tggccgcgat tgcattggtt caccgcaaaa ccaccaatcc gaaacgtatg gatccagccg
    12781 accaagttaa agtacgcgcc gatcaaggcc gtatgcgtct ggtgaaaatg gaagcggtca
    12841 aaccgcaagt cgaatctgcc gaagaaagcg aagtttcaga cggcctcaag acggaagggg
    12901 agggcaaagc atgattactt tgacgcatta tctggtattg ggcgctctcc tgttcggtat
    12961 cagcgcgatg ggcatcttta tgaaccgcaa aaacgtgctg gtattgctga tgtccatcga
    13021 gctgatgctt ttggcggtga acttcaactt tatcgccttc tcgcaacatt tgggcgatac
    13081 tgccggacaa attttcgtat tcttcgtatt gaccgttgcc gctgccgaat ccgccatcgg
    13141 tttggcgatt atggtgctgg tgtaccgcaa ccgacaaacg attaatgttg ccgatttgga
    13201 cgagttgaaa gggtaattat gaacgatatg actttatatt tgataattgc ccttgttccg
    13261 ttggcaggct cgttgattgc gggtttgttc ggcaacaaaa tcgggcgtgc cggcgcgcat
    13321 acggttacga tactcggtgt cgctgtatct gccgtgctgt cggcttatgt gctgtgggga
    13381 ttcctcaatg gcagccgtgc caagtttgat gaaaacgtct atacctggct gacaatgggc
    13441 ggtttggatt tctccgtcgg cttcttggtc gatacgatga cggcgatgat gatggtcgtg
    13501 gtaacgggcg tgtcgttgat ggtgcatatc tataccatcg gttatatgca cgatgaaaaa
    13561 gtcggctacc aacgcttctt cagctatatt tctttgttta cattcagcat gttgatgctg
    13621 attatgagca acaacttcat ccagctcttc ttcggttggg aagcggtggg cttggtgtcg
    13681 tatctcttga tcggtttcta tttcaaacgc ccgggcgcga catttgccaa cctgaaagcc
    13741 tttttgatca accgtgtcgg cgacttcggc tttttgctcg gtatcggctt ggtgcttgcc
    13801 tatttcggcg gcagcctgcg ttatcaggac gtattcgcct atctgcccaa cgtacaaaat
    13861 gccactatcc agcttttccc cggcgtggaa tggtctttga ttaccgtaac ctgtttgctc
    13921 ctgtttgtcg gtgcgatggg taaatcggca caattcccgc tgcacgtctg gctgcccgat
    13981 tcgatggaag gcccgactcc gatttctgca ttgattcacg ccgcgacgat ggttaccgcc
    14041 ggtttgttta tggtgtcgcg tatgtcgccg atttatgaga tgagcagcac cgcgctgtcg
    14101 gtcattatgg taatcggcgc gattaccgcc ctgtttatgg gcttcttggg tgtgattcaa
    14161 aacgacatca aacgtgtggt tgcgtattct accctgtcgc aactgggcta catgaccgtg
    14221 gctctgggcg cgtccgccta ttctgtggcg atgttccacg tcatgaccca cgccttcttt
    14281 aaagccttgc tgttcttggc ggcgggcagt gcgattatcg gtatgcacca cgatcaagat
    14341 atgcgccata tgggcaacct gaaaaaatac atgccgatta cttggctgac tatgctgatc
    14401 ggcaatttgt ctctgatcgg tacgccgttc ttctccggct tctactccaa agattcgatt
    14461 atcgaagcag tgaaatacag caccctgccg ggcagcggct ttgcctattt tgccgtcctc
    14521 gccagcgtgt tcgttaccgc gttttacgcg ttccgccaat actttatggt gttccacggc
    14581 gaagaaaaat ggcgcagtct gcccgaacac cattcagacg gccatggtga agaacatcac
    14641 ggcttgggca aaaacgacaa tccgcacgaa agcccgctgg tcgttaccct gccgctgatt
    14701 ctgcttgcga ttccgtccgt catcatcggc tacatcgcca tcgaacccat gctctacggc
    14761 gatttcttca aagacgtgat tttcgtcaac gccgacgcgc atccgaccat gcacatcatg
    14821 aaggaagagt tccacggcgc attggcgatg gtgtcgcaca gtctgcattc gcccgtcctc
    14881 tatctcgcta ccgcaggcgt attgagcgca tggcttttgt acatcaaact gccgcacctg
    14941 cccgctaaaa tcgcgcaggc gttccgtccg gtttacgttt tgtttgaaaa caaatactac
    15001 ctcgacgcct tgtatttcaa cgtcttcgcc aaaggcacgc gcgcattggg aaacttcttc
    15061 tggaaagtcg gcgataccgc cattatcgac aacggcatcg tcaacggctc cgccaaactg
    15121 gtcggcgcaa ttgccgcgca agtgcgtaaa gtccaaaccg gctttatcta cacctacgcc
    15181 gccgctatgg tgttcggcgt attggtattg ctcggcatga ccttctgggg attgttccga
    15241 taaggatgag gcttcagacg gcctgcttat cgctggtgta gaggtcgtct gaaaagaagg
    15301 tttcaggcgg caatagatta gagtttcaga cgacatctga catttcagat agtcaggccg
    15361 tctgaaaaat agaagggcgg ataatatgga taaacggctg tttgacggta cggttcgttt
    15421 tgagcatgta accggtgtcg gcacgatgca ggtagatttc cagcccaatc agcgtgttta
    15481 tacgatgatt ggcgagaatg gtgtcggtaa gaccaaattt ttggaatgtt tgtttacggt
    15541 attgttgttt acttatcgaa tggcgaacat aaacataggt tttagttgga tagatttgca
    15601 acgtttacct ttgaaaatag ttgaattgcc taatggaaaa aatgaggatt ttaggcgctt
    15661 ttcagagcgt agtaatcagg gctttaatat taacgaagac gttttgttct caggatatca
    15721 acatcgactt ccttttgtat atttgccggc acagagtcgt gggtgggtaa aaaaacaaac
    15781 ttccaaactc agtcgctggg aagttctcaa gggcgtgcaa gcaaatatct ggggaatttg
    15841 ttttctgttt ttcaatctgg ccatagttca ttctcgctta aatccttaaa tatggaaaac
    15901 gatttagaag aatggattat ccagcgcgcc cgatcagcca atccgtatca ggcaaacgaa
    15961 gataatcgaa aaattgaaat tgatacttta ctgtttcttc ttaatcaatt tgatggtcgt
    16021 attgcacctg attttttaga aattaaagga gatgatcgta tattcatcaa gttggataat
    16081 caaaaacgcg aattgtcagg attatccagc gggtttacgt ctattttgaa aattatgcaa
    16141 tctattgttg cgggatacag ttactttacc aatgagcagc aaattgccaa tgtgcgcggc
    16201 gtggtgctga ttgacgaaat agaaagccat ttgcacaacg aatggcagat caaaatcgtg
    16261 ccgctgctga aaaaattgtt cccgaacaca acgtttttca ttaccacgca ttcttcttta
    16321 gtcatcagtc agttagagca gggaggggcc tatcgtttgg tacgtagcaa ggaggacggt
    16381 gtagtctatg gcaagatgat tgactatcca agtaatgctt cttttgtgga tttgctcaac
    16441 gaagcattcg gcgtggattt gaataagaag aagatagaac gcgcccaaga acgtaatcaa
    16501 gaacaggcaa aaaagcctta ttggaattgg tacgtcagga attgggcgga ttaggagata
    16561 aataatgacg gtttatcttt tagataccaa ttacttggtt tatttggcgg atgatgacag
    16621 tgacgaagaa aagcggaaag ccgtattgag cgatatggcg gaaaagttgc agcaggatga
    16681 caaccgtttt gtgattaccc cgcttatccg ttatgaagtt ttgcgcggtg tggattgggg
    16741 aaaatctgaa aaattgtctc gattgaccgg tgttttggca cagttttagt ctttggacat
    16801 tacacagaat gtttctgatt tggcaagtaa tctgtaccgt tttgataagt ttgaagccga
    16861 acgtgataac acgccgaaga atttagaaaa acgtaaattc gatatgtttc attatgcgac
    16921 tgccagtgtg aataatttag aaatcttatc ccacgatacc gatgttaata agattaaaga
    16981 tttacatgaa agaatgaggc tggaagattc ggcagaatta gcttgagaca aatagcagaa
    17041 acctgaacaa acctttttca gacggcctct taaaacgtcg tctgacaaac caacccgtac
    17101 aacgatattt ttattttaac cacaggttaa ccactatgtt ttccaactac ctactcagct
    17161 tggcaatatg gatacccatc gccgcaggcg tgctggtttt ggcgaccggt aaagacagcc
    17221 gtgcgccgct ggcgcgtgtg cttgccttca tgggagcgct tgccggtttc ttggtaacgc
    17281 tgcccctgtt taccggtttc gaccgtttga gcggcggcta tcaatttacc gagttccacg
    17341 agtggattcc gcttctgaaa atcaactacg cattgggcgt ggacggtatt tcagtgctct
    17401 ttatcatctt gaatgcgttt attacgctgc tggtggtatt ggccggttgg gaagtcattc
    17461 agaaacgtcc ggcgcagtat atggcggcat tcctgatgat gtcgggtttg attaacggcg
    17521 cgtttgccgc gcaggatgcg attctgtttt atgtgttctt cgagggtatg ctgattccgc
    17581 tgtacctgat tatcggtgta tggggcggcc cgcgccgcgt ctatgcgtcg gtcaagctgt
    17641 tcctctatac gctgacgggt tcgctcttga tgttggttgc catggtttac ctgtactatc
    17701 agacaggcag cttctctatt gtcgatttcc aaaacatcaa acagattccg ttgggcgtac
    17761 aacagctttt gtttgtagcg ttcttcctgt catttgccgt aaaagtgccg atgttccccg
    17821 tacacacttg gctgccggat gcccacgttg aagcgccgac cggcggttcg atggtgttgg
    17881 cggcaattac gctgaaactg ggtgcgtatg gtttcttgcg ctttatcctg ccgattatgc
    17941 cggacgcggc tcgctatttt gcccccgtga tcatcgtatt gagcctgatt gccgtaattt
    18001 atatcggtat ggtggccctg gtgcaaaccg atatgaagaa actggtggcg tattcgtcca
    18061 tcagccatat gggttttgta acgcttggga tgtttttgtt tgttgacggg cagttggacg
    18121 actgggcgtt gaaaggcgcg gtcattcaga tgatttcgca cggcttcgtg tcggcggcga
    18181 tgtttatgtg catcggtgtg atgtacgacc gcctgcacac gcgcaatatt gccgattatg
    18241 gcggcgtggt caatgtgatg cccaagtttg cggcgtttat gatgttgttc ggtatggcga
    18301 acgccggcct gcctgcgact tccggcttcg tgggcgagtt tatggtggtt atgggcgcgg
    18361 tcaaagtgaa tttctgggtg ggcgcgctgg cggctatgac gctgatttac ggcgcgtctt
    18421 ataccctgtg gatgtacaag cgcgtgattt tcggcgcgat tcacaatccg cacgttgccg
    18481 aaatgaaaga catcaattgc cgcgagtttg cgattctggc ggttttggcg gttgctgttt
    18541 tggggatggg cctgtatccg aacgcattta tcgaagtggt gcatcaggcg gcaaacgatt
    18601 tgattgccca tgtggcgcaa agcaagattt gaggtgtgta aatgaattgg tcagatttga
    18661 atttaatgcc cgcactgccc gaagtcgcgc tgctgtcgct actggtgtta ttgttgccgg
    18721 cggacttgtg ggccagtgat gacaaatgcc gttggacgca ttacggcgcg ttggcaacgg
    18781 tggcggttac ggcggcggtg cagcttgccg tgtgggaaca gggcagcacg tcttcgttca
    18841 acgggatgta tattgcggac ggtatgtcgc gtttggcaaa aatggtttta tatgccttga
    18901 cgtttgtcct gtttgtctat gccaagccct acaaccaagt gcgcggtatt ttcaaaggcg
    18961 agttttacac cctgtcgctg tttgccctct tgggcatgag cgtgatggtg agcgcggggc
    19021 attttttaac tgcctatatc ggtttggaac tcttgtcgct cgccctttac gccctgattg
    19081 ccctgcgccg cgattccggt tttgccgccg aagccgcctt gaaatatttt gttctgggcg
    19141 cgctggcatc cggcctgctg ctctacggta tttctatggt ttacggcgca accggttcgc
    19201 tggaatttgc cggcgtgctc gcctcttcct tcaatgaaga agctaacgaa tggctgttga
    19261 aactgggtct ggtgtttatc gtcgtcgccg ttgcgttcaa actcggcgcg gtaccgttcc
    19321 atatgtggat gcccgacgtg tatcacggcg cgcccacttc tgttaccgcc ttggtcggca
    19381 ctgccccgaa aatcgccgcc gtcgttttcg ctttccgcat cctcgttacc gggctgggaa
    19441 ccgtgcatca tgactggtct ctgatgtttg ccctgcttgc cgccgcctcg ctgctggtcg
    19501 gcaaccttgc cgccatcatg cagaccaata tcaaacgtat gctcgcctat tccaccgtat
    19561 cgcatacggg tttcatcctg ttggcgttta tggcgggcgc ggtcggcttt gcggcgggcc
    19621 tctattacgc cattacctac gcgctgatgg cggcggcagg gttcggcgtg ttgatggtgt
    19681 tgtcggacgg ggacaacgag tgcgaaaaca tcagcgattt ggcagggttg aaccaacacc
    19741 gcgtatggct tgcctttttg atgctgctgg ttatgttctc tatggcgggc attccgccgc
    19801 tgatgggttt ttacgccaaa ttcggcgtga ttatggcgct tttgaaacaa ggctatgtat
    19861 ggttgtcggt gtttgccgtc gtgatgtcgc tggttggcgc gttctactac ctgcgcgtgg
    19921 tcaaagtcat gtattttgac gaatccggcc gcgcccgtcc cgccgccggc ggcaacaatg
    19981 ccgcaaaatc cctcttaagc gtcaacgcgc tgctgttggt gctgtggggc attatgccgc
    20041 aaaccgttat cgactggtgc gccaaggcgt tggagaacac gctgtgagcc gccgaaacgg
    20101 cagccgtgtc agaggctgcc gtttttgtta agatatgccg ttccgcaatg cagttcagac
    20161 ggcatcgccg ccgacaatgc ccaaacagaa agcccatcat gaccgcatcc atgtacatcc
    20221 ttttggtctt ggcactcatc tttgccaacg cccccttcct cacgaccaga ctgttcggcg
    20281 tggccgcgct caagcgcaaa catttcggac accacctgat cgagctggcg gcaggtttcg
    20341 cgctgaccgc ctctcttgcc tacatcctcg aatcccgtgc gggagcggta cacaatcagg
    20401 gttgggagtt ttacgccacc gtcgtctgcc tgtacctcat tttcgccttc ccgtgtttcg
    20461 tgcggcggta tttttggcac acgcgcaaca gggaataaac aagcaaacaa gcataggaat
    20521 gccgtctgaa accctttcag acggcatttg tttcattcaa gtgcaggccg gcatcgctgt
    20581 gccggcacgt ttcagtcggc gatatacgcc ggttttaata tttgcgtgcg actgcaaatt
    20641 ctgccaactg ccgcaggcgc agggctttgt cgccgaaggg ttcgagcagc gcgactgctt
    20701 cggcaaccag tttgtgtgcg tatgagcgcg ccgcttccaa gcccatcagt ttcacataag
    20761 tcggcttgtc gttgtcggca tctttgcccg ccgtcttgcc caaagtcgcc gtgtccgctt
    20821 cacaatccaa cacatcgtcg atgacttgga acgccaaacc cagttttgcc gcgtaagcgt
    20881 ccaatacggc aagttccgcg tcggacagat cgggacacgc cgtcgccccc aataaaaccg
    20941 ccgcacggat caacgccccc gttttcaggc tgtgcatccg ttccaaatcg gcttgaacca
    21001 tttgtttgcc gacatttgcc aaatcgattg cctgtccgcc cgccataccc gcgctgccgc
    21061 ccgcttttgc caacaccgac aacattgcca actggcgtgc ggcgggcagt tctgtcggac
    21121 ggctcaacac gtcgaaggct tgggtttgta aagcgtcgcc ggtcagaagg gcggtggctt
    21181 cgccatattt gatgtggcag gtcggtttgc cgcgccgcag gctgtcgttg tccatcgccg
    21241 gcatatcgtc gtgaaccaaa gaatagacgt ggatcatttc gattgccgcc attgcctgtc
    21301 cgaccgcatc cgccacggcc ccgcccaatt ccgaagccgc cagcaccagc atcgggcgca
    21361 gacgcttgcc gccgtccaaa gccgcatagc gcatcgcttc gtgcagcgtg tgcgggattt
    21421 cgtttccaga cggcaaaaag cgttccagca gcagctctgt ctgcgcctgc gccctctgtt
    21481 gccacgcttt caaatcattc gtcggattca aggttcaact ccttcgtccc gtccgcatcc
    21541 aaaacctgta atttctgttc gacttgggcc agcttggttt ggcagtacct gaccagctcg
    21601 ttgccttcct gataggcggc aagcgcgtct tccaagggca tttcgccctg catggactgc
    21661 gtcagcgatt caaggcgcga caaggcctct tcaaacgatt tcggggtgtt tttcttcata
    21721 gtttcgggtg ttccggtaaa aagcggattg tagcattttt gcaaaacgga tgccgtctga
    21781 aacccgaatc cggcttcaga cggcattttt tccgccccgg tcggcaaggc gttacccgag
    21841 cagttcgtcg gtgatgccct gcaaaaaggc gaggcgttcg gggcttgccg ccccggtttc
    21901 ggcggcggct ttgaaggcgc agccgggttc ggcgcggtgg gtgcagttgt ggaagcggca
    21961 ttgcccgaca aggtggcgga aatcggggaa atagtgcggc aaatcggcgg cttggaggtg
    22021 gtgtaaacca aattcttgca aacccgggga gtcgatgagt tgggtttcgc cgttcaaatc
    22081 ataaagccgg gcgtgggtgg tggtgtgttt tcccgagtcg agtgcggcgg aaatgtcgcc
    22141 ggtgcgggcg gtttggctgc ctaaaagggc gttggtcagg gtggatttgc ccataccgct
    22201 ttgtccgagc aggatgttgc tgtgcccttg caggacgggg cgcaggctgt cggcgttttc
    22261 cagcacgcgg gtttcgatga cggggtaacc cagcgtttcg tagaatttga gtttttcgag
    22321 ccagagggcg gtttcgggca ggtcggcttt gttcaggacg atgacggcgc ggatgccggc
    22381 ggcttcggcg gcaagcaggg cgcgctgtag cagccgcacg ctcggactcg ggacggcggc
    22441 ggttacgatg aggagttggg taacgttggc ggcgatgagt ttggttttcc acgcgtcttg
    22501 gcggtagagc aggctttggc gcggtaaaaa atcttcaatc acaacttgtt cggcgttgac
    22561 ggggctgatg cggacgcggt cgccgcaggc gaaatcgacg cgttttttgc gggtgctggc
    22621 ttcgtaggtt gtgccgtcgg gcgtgcggac gatgtagcgg cggccgtagc cggcggtaat
    22681 ttgggcggtg tcgttcatgg tttctttggg gttgggtgtg gaaatgccgt ctgaaaaggg
    22741 gtgttcggac ggcatcggtt cagtcgtgct gccactcgac gtgttcgctg aggaagccgc
    22801 cgctctggtg cgcccagagt ttggcgtaaa gcccgcgttt ttcgaggagt tcggcgtgtg
    22861 tgccttcttc gatgatgcgg cctttgtcga ggacgacgag cctgtccatc gcggcaatgg
    22921 tggagaggcg gtgggcgatg gcgatgacgg ttttgccttc catcattttg tcgaggcttt
    22981 cttggatggc ggcttcaact tcggaatcga gcgcgctggt ggcttcgtca agcagcagga
    23041 tgggtgcgtc tttgagcatc acgcgggcga tggcgatgcg ctggcgttgt ccgccggaga
    23101 gtttcacgcc gcgttcgccg acgtgcgcgt cgtagccgct ccgccctttg gcatcggaaa
    23161 ggtcggggat gaagccggcg gcttcggcgc gttcggcggc ggaaaccatt tcggcatcgg
    23221 tcgcgtcggg gcggccgtaa ataatgttgt cgcgcacgga acggtgcagc agcgaggtat
    23281 cttgtgtgac caaaccgatt tgggcgcgta aagattcttg ggtaacgccg cttatgtcct
    23341 gcccgtcgat cgaaaccgtg ccgctttgcg gttcgtagaa acgcaaaagc aggttgacga
    23401 tggtggattt gcccgcgccg ctgcgtccga tcaagccgac tttttcgccg ggtttgatat
    23461 tcaggttaaa gccgttgagc agcggtttgc cggcttcgta gcaaaaatcg acgtgttcga
    23521 acttgattgc gccttgcggc acgttcagcg gcagggcttg gggcttgtcg aggatggtgt
    23581 gcggtttgga cagggtcgcc atgccgtcgt tgacgatgcc gatgttttca aacagccgcg
    23641 cggattccca cataatgtat tgcgacagcc cgttgacgcg caacgccatc gcggtggcgg
    23701 tggcgaccgc acccacgccg acctgtccgt tgtgccaaag ccagatgccc agtgcggcgg
    23761 cggagagggt cagcgaagtg ttgacgatga agctgcacga atgcagcaga gtcgccagcc
    23821 gcatttgggc gcgcaccgta accataaatt cttccatcga ctgcttggca tagacggctt
    23881 cgcgcgcgcc gtggaaaaag agtttgacgg tggcgatatt ggaataggca tcggtaatgc
    23941 ggccggtcat cagcgagcgg gcattcgcct gccgtgcggc ggtttgcccc aatctgggaa
    24001 tcagcaggcg cattaccgaa gcgaaaccga ttatccagcc gataaagggc agcagcagcc
    24061 atgagtcgag cgaggcgaga atcacgcccg aagtgatgaa atacaccgac acataaacga
    24121 ccatatcggc aaccgtcatc accgcgtcgc gcaacgccag cgcggtctgc atgactttgg
    24181 cggacacgcg gccggcaaat tcgtcctgat aaaaaccgag gctctggttc agcatcaggc
    24241 ggtggaagtt ccagcgcagg cgcataggga acacgccctg aagggtttgc aggcgcacgt
    24301 tggacgcggc aaacgcccac acgaccgaaa acgccatcat cgccgccatt gccgccagtt
    24361 cccaaccttt ttcggcaaac agttcggcgg gcgcgtattt gccgagccac tctacgattt
    24421 tgcccataaa ttgaaaaatc agggcttcca taatgccgat gccggcggtc agcgcagcta
    24481 gggcggctat ccatttccgc accccgtcca tactgctcca gacaaaccgg cacaagcctt
    24541 tttcgggcgt tttcggcgcg gcttcggggt aggggtcgat tcgggactcg aaccaggaaa
    24601 atattttgtt caacattgtt ttcgatttcg gtaaaacagt ttcagacggc atcaaacaca
    24661 atgccgtccg aaaggaggga caataacgcc attttacggg aaaagccgtc gggaagacag
    24721 cgcggggcgg aaacgcaggg tttcgtcagg gcaaacgccg cgccgccttc ggacggcatt
    24781 atttcagcag gtttttcagg gcaaggcgca cgccttcgcc cacgtccgtc cccttcggaa
    24841 cgcctttgac cgccgctttt gcctcgcgtt cgttgtaacc cagcgcaagc agcgtgctga
    24901 cgatgtcttc cgtttcgtcg gcggcgggtg aggcggcaaa cagcccgtcc gttaccgtat
    24961 gcgcgaccag cttgccgcgc agttccaaga ccatacgttc ggcggttttt ttgccgattc
    25021 ccggagcgga ggagaggcgt ttgacatctt cttctgcaac cgcccgcgcc agctcgtctg
    25081 ccgtcatcgc cgacaaaatg cccaaagccg ttttcgcgcc gatgccgccg accttaatca
    25141 gttggcggaa ggtcttgcgt tcttccgcag tggcaaaacc aaataaaaga tgtgcgtctt
    25201 cccgaatgat aagctgggta aacagttgta cgctttcacc cacgggcggc aggttgtaga
    25261 aggtctgcat cgatacgtcg gcttcataac cgacaccgtt gacatcgatg acgatttgcg
    25321 gagggttttt ttcaaccagt ttgccggtca gtctgctgat catgtgtgcc gaatcctgaa
    25381 gtgtcgggtg caaaatgccg tctgaaaccg gttcgggctc cagacggcac ggattgtatc
    25441 aaattcagtc gtcgcggcgg gaggaaatca cgcggccggt acgggcatcg acaacgactt
    25501 tgtattcctg tccgtttttg acgatttcga catcatagtg cggacggccg tcgtcgtgtt
    25561 cgagatcgat gtcggtgatt ttgccgccga cacgcgccca agccgctttt tcggcttggg
    25621 cgcggctgat gattttgtct tgtttgccgt gttggtgtgc ggcgtgtccg tggtcgtcat
    25681 cgccgtgtcc gtcgtggtgg gcgagtgcgg gggcggaaat gcccaacagt gcggttgcgg
    25741 cggcggtcaa gagaaggtgt ttgatgttca tatttgccct tgtaaatcag gggttgaaaa
    25801 atggggatat taataaggta tcgaataacc gtcaaccgga ggtcagtacc gcccgaacca
    25861 taccgcgtgc ctgcgcttcg gcttcggcgg cgcgttcctg cgaggcaaac gggcccattt
    25921 tgacaacgta ttcgtaacgg cgtttttcaa ccgagaggtt cgggcttgat gacgaagcgg
    25981 cgaaattttg ggcggcttgg ttcagatagg cttgtgcttc gtgttccgta ccgaaagatt
    26041 tcaagtcgat aaagatgtct ttgttttcgg caaccggtgc ggattggccc gggacgattt
    26101 gttcgatttt gacgtgtgcc gtcccttggc tgacaaagcc caatttttgc gcggcggctt
    26161 tggatacgtc gatgatgcgg ttgccgtgga aggggccgcg gtcgttgacg cggacgatga
    26221 cgcttttgcc gtttttggtg ttggttacgc gcacatggct ggggatgggc agggttttgt
    26281 gggcggcggt aaaggcgttc atatcgtagc ggtctccgcc ggaagttttg cgcccgtgaa
    26341 acctgccgcc gtaccacgag gcgttgccgg tttgcgtgaa ttcggcgact ttgtagctgc
    26401 ggttggcgga ggcgtgcagt ttttccggct tgactactgc gtcggcggat gccgtctgaa
    26461 gggagtgtgt gccgaacgcg gcggtgagga ggaaaagggt ttttcgggtt aaagtcaaaa
    26521 tgtgttccgt tcttgagttg aagacggatg ggcattatgc cctacggacg cgttccgaac
    26581 cgccgcacgc tgcggcagcg gttcggaatg tgtccgaacg gttttcagac ggcatgaacc
    26641 tgcgttcaaa cgccgcctgc gtaaccgtgt tgccgccacg cttcaaagag aatcacggag
    26701 acggtgttgg aaaggttcat actccggctg ccgggccgca tcggcaggcg gattttttgc
    26761 gcggcgggca ggctgtcgag gatgtcggca ggcagcccgc gcgtttccgg cccgaacagt
    26821 aaaacgtcgc ctttttgaaa cgcggtttca tcggggcgcg ccgtgccttt ggtggtcagg
    26881 gcgaaaatac gtcggcctgc cagcgacttg aggcagtcgt cgaagttttc gtgcaccgtc
    26941 aggctggcga attcgtggta gtcgagcccg gcgcgtttca ttttggcgga atccaatggg
    27001 aagccgagcg gtttgacaag gtgcaaatcc gcgccggtat tggcgcacag gcggatgatg
    27061 ttgcccgtgt tcggggggat ttccggctga tataaaacga tggtaagcat aaatatcaat
    27121 cacttatagg cgcgtaacct tgccataagg cggatggggt gtcaaaaaat ttagttattt
    27181 tttcattggc gtgcgtgcca gcgtccagca gcagattcgg tttgcgcccg attttttcag
    27241 cgtctttgcc aattcgtcca gcgtcgcgcc ggtggtaaag acatcgtcga ttaacaaaat
    27301 attacagttt tccggtatcg gtgtgcggat ttcaaaggcg tttttgatgt ttcgccgccg
    27361 ttcgccgcct ttgagcgtgc tttgcggcgg gcggtggtgt cggaatacgg tgtgtcgggg
    27421 cagtatctgc cagccgtagc gttgcgccaa caacccgacg atgctctcgc tttggttgaa
    27481 cccgcgttgc agcagccgct ccctgcttag cggaacgggt aggacgaaat cgaaacattc
    27541 gtctgaaagc cggtcgggcg gattctgcat cgtcaggtct gccagcggct gcgccatgcc
    27601 caaatcagcc aagtgcttca gcgcgcgtat catattgctg acgggcggtt cgtaatgcag
    27661 cgatgcccac atccggtcga atgcgggcgg ttttttctga catccgccgc acaccgcgcc
    27721 gccttgaacg tgcctgaaac acagggggca gctgtttgcc gcgtcggtgc ggaaacttgc
    27781 caaatcggta ttgcagccgg cgcagatgcc gtctgaaacg ccggacgaac cgtggcataa
    27841 tacgcaacgc ctgatagtgg gcgcgtctgc gatgcgccgc caacaagaga gaaaattcat
    27901 gcctgatgct gccaaaaaag tttacctgat acacggttgg ggggcgaacc gccacgcgtt
    27961 cgacgatttg atgccgcgcc tgcccgcaac gtggccggtg tccgccgtcg atttgcccgg
    28021 acacggggac gcgcccttcg cgcaaccctt cgatattgag gcagctgccg acggcattgc
    28081 cgctcaaatc gatacgtcgg ccgacattct cggctggtca ctcggcggat tggtcgcgct
    28141 gtatctggcg gcgcgccatc ccgacaaagt ccgttcgctc tgcctgacgg cgagtttcgc
    28201 acggctgacg gctgccgaag actatcccga agggcttgcc gcgcctgcac tgggcaaaat
    28261 ggtcggcgcg ttccgaacgg attatgccaa acatatcaaa cagtttctac aactccagct
    28321 tctgcacacg cctgatgccg ccgaaatcat cggcagaatc ctgcccgatt tggcgcgctg
    28381 cggcacgcct caagccttac aggaagcttt ggacgcggcg gaaagggcgg atgtgcggca
    28441 tttgttggac aagatagatg ttccggtact gctggtgttc ggcggcaaag acgcgattac
    28501 accgccgcgt atgggcgagt atctgcatcg gcatttgaaa ggaagcaggc tggttgtgat
    28561 ggaaaaggcg gcacacgcgc cgtttttgag ccatgcggaa gtgtttgccg cgctgtaccg
    28621 cgactttgtc gaagggggtt tgacatgaat ccgcaggaca aatgctggca ggttcaccgc
    28681 catcttgccg aacataccga ccaacgcctg acactcgtcc gcaacgcgcc cgggcatatc
    28741 ctgcttgccg gtgcggatgc ggacatcagc cgcagcctgt tggcgaagcg ctatccgctg
    28801 gcggtatttg aagaatacga ttcccgtgcg gattttttgg cggctgccgc agccgcgcgc
    28861 aagggcggtt tctggcaaaa actgacgggc aggggcgtgg tgcagcactg ccaatccccg
    28921 accgtgccgc tgcccgaagc gtgtgccgat atgttgtggt cgaacctcgg actgttggcg
    28981 gcggaacaaa tccttcccgt gctgcacaac tgggcgcgcg cgctgaagac ggacgggctg
    29041 ctgtttttca cctgcttcgg gcgcgatacc ttggcggaac tgaaatcccg tctgaaagaa
    29101 aacggcattg aaagccgcag cgtgatgttt cccgatatgc acgacttggg cgatatgctc
    29161 gccgaaaacg gcttttacga tccagttacc gatacggcga agctggtgtt ggactacaaa
    29221 aaggcggaaa cgttttgggc ggatatggac acgctgggcg tttggcgggc ggtggcgtgg
    29281 gacgacgaaa acgccgcgcg ttcgtgtgtc ggggcgatat ttgaacggga aggcggtttg
    29341 ggcattacgc tggaaacggt gtacggacac gccgtgaaaa aactggtgct gccgcagggg
    29401 gagaacgtgg tgcgtttttt tccgaagaga tgatgtgcag atgacgtctg aagccgtttc
    29461 caagtttcag acggcatttg tctgtgaaaa ccgacagaaa taaaggaaat gccgatgtat
    29521 agtgaattaa atttaaaccg gtacggcgtt ggctcgcctt gccgtactat ctgtactgtc
    29581 cgcggcttcg ccgccttgtc ctgatttaaa tttaatccac tatgtgccga tgccggagac
    29641 gtatattgcg tctataacat cagactgaag cagtacactg cctgccaggt tacccgagtt
    29701 gaagaacacg gtggcaaaaa aacacatgcg accctgctgg ctttggactg gcggggcaac
    29761 aaaccgcttg gggcggcgga gctggcggat ttgaaaccgc tttacaaaga ctttatgtat
    29821 tgggaacgcg gtctgcacat gtataaggca agtgccgtcg tgccgacggg atatgtacgg
    29881 gttgggaata ccgcgccgct gtgcggcgaa gacacgcagc ggtatgcctc tttttggggc
    29941 gacggctacg acgtgtaccg tcagttgaga tggcggcaga tacccgaaaa acagagaaag
    30001 gcattcaaaa aagccgccaa aagcaaaaat accgtgatgt ttgccggacg ggaatacggc
    30061 atatccaaac agaatttgag cgatgtttgg gatgattttg aagacgcgat ggaactgaag
    30121 gcgtttccct gcctgtcttc gctgtttctg accaagtggc ataaaaatct atatgaatat
    30181 ctggaggaat atccgtttat cacccgattg tgtctggaaa accacggtca gaccgtgctg
    30241 gatttcagca atacgcgcat aaccgacctt tctgtcgata tgaccggcgt ggaatcgctt
    30301 tatttgaacg aaggcttgga cagcctgaac ctgaaaggcg aaatcaagga aaactgcaag
    30361 gtctgtaccg ccggaaaggg tgcgggtctg attttggaag tgggcaaaag cgttcccaaa
    30421 gtgcggggtt tggaaaacct gaccgccgtc aacgtcatgg gcattgccga tttcgatatg
    30481 caaaacctgt cggaaacgta tcccaagctc aaaaccatac ggctgtgggg caaaccgggc
    30541 aatatcgcca atttttccgc cgtatcggga tttgaggatt tggaagtgtt cactgccgta
    30601 gatttgttcg gtttcggcgc ggacgacatc cctcatcccg accggctgcc gaagctgcac
    30661 aggctgtgga tgagcagcct gcccgaagag gcggcgaagg cagtgaagaa actttataag
    30721 aaacggaagg aagacgggct ggacctgtgg atagaaaaag cccgaaaacc cgaatggctg
    30781 gcgcaaaatt tcgacaaccc cttccgcgat tgggacggcg cggagcatat cccgaaaagc
    30841 catgccaaaa aagcggcgga gctgtaccgg aaaacccgtg ccggcgtggt caaactgctc
    30901 ggcaatccgc ctgaaaacac aggggagggg ctggcagagg cggtcaaagc ctataccgga
    30961 ggtttcaaca aaatggacaa aaaacacttt atcgacacgg tagagcggga agatattgcc
    31021 gaggcgttgg aaacgatatt ggacctgata ccggatgggt cttgtgccga caaggaaaaa
    31081 ctgtttgaaa tatttgataa aaacaggaat ttttaaacag aggcaatgcc gtctgaaact
    31141 tggtaacggg cttcagacgg catcccgttc caacaccgcc aacaccgccg caccgtaacg
    31201 tgcggctttt tcttcgccta cgccgtatac ggcggcaagc tccgccaaac cttccggctg
    31261 tttggcggca atggcgcgca gggcggcttt gctgagaatg cggtagggtt cggactgttc
    31321 gtgttttgcg gtttcgttgc accattgaat cagggcgcgc atcaggcagc gtttgtgttt
    31381 ggcggtttca tcgatgccgt ctgaaaacgg acggcagacg gcgaggatgt cccgtccgta
    31441 tttggcggcg cgtacgctgc ccaagccgtg tacggcttcg aggtcggttt cggtttcggg
    31501 cgtggcggca agcatatcgg caaggctttc gtcggagagg acggtatgca gggcgcagtt
    31561 ttccgccctt gcctgttcat accgccaggc ttcgagtttt tgacgcagtt gttgttcgcg
    31621 ttcggtttgc ggacggatga cggcatcgtg gctaaagtcg gcagcgtttt ggcagatttt
    31681 gagtatgccg tgtccgaaac gttcggtttt ggcttcgccc aagccgtaga tgtcgtgcag
    31741 cccgttgagg ttttgcggca ttttttcgac aaggtcgcgc agggttttgt cgccgaaaat
    31801 catataggcg gggatgcctt cggcttcagc ctgtttcatg cgccaaacgc gcaatgcctg
    31861 ccacaggcgt tcttcgcgtt cggtacgcag ccagttgtct ttgagggtgc gggtggcggg
    31921 cttgtcgcgc ttgagcgggc gcagcatcac ttcggtttcg cctttgagga cttttttggc
    31981 ggcttcggtc agttgcaatg cctgatatcg ggcaatgttg acggtgaggt agccgaggct
    32041 gatgcactgg cggatgacgc tgcgccattc tttgtcggac aattccgcgc cgatgccgaa
    32101 tgtggacagt tgctcgtgcc ggttgccgcg tatccaatcg tcgcttttgc cgcgcaaaag
    32161 gttggtgatg taaccggcgg caaagcgttg tccggcgcgg tacacgcagc tgagtaattt
    32221 ttgcaccaac accgtgccgt caaaccgtac gggcggatgc aggcagttgt cacaatgtcc
    32281 gcagggttcg gatgcttcgc cgaaatgttt gagcagcagt acgcggcggc aggcggcggt
    32341 ttcgcagacg gaaagcatgg catcgagttt ttgcatttcg atttgctttt gcacctcgcc
    32401 gctgttgcct tcggcaatcc gttcgcgcag caacacccaa tcgttcaagc cgtaacacag
    32461 ccaactcacg gcaggcagcc cgtcccggcc ggcgcgccct gattcctgat agaaatgttc
    32521 gacactctgg ggcatatcga gatgggcgac aaagcgcacg tcgggtttgt ctatgcccat
    32581 gccgaacgcc acggtcgcca ccacgataat attgtcttca tgcgtaaagc ggcgttggtt
    32641 ttcctcgcgc acgtccatgc tcaaaccggc atgatacgga atcgcgttta atccgttttc
    32701 acgcaaaaac tgcgccgcat cttcaacctt tttgcggctt aggcaataca caatgccgct
    32761 ttgccccgtc atttctttgc ggatgaaatc cagcaattgt tttttgccgt tgtttttttc
    32821 gataacctga taataaatat tcgtgcggtc aaagctggag acaaattcgg acgcgtcgtc
    32881 caagtgcaga taatgcttga tgtcggcgcg cgtggcggca tcggcggtgg cggtcagggc
    32941 gatgcgcggg atgttcggat agcgttcggc aagcataccg agctgttgat attcggggcg
    33001 gaaatcgtgt ccccaccggc tgacgcagtg cgcttcgtca atggcgaaca ggctgacggt
    33061 ttgttggtcg agaaaacgta aaaagcggtc ggtaaccaag cgttccggcg cgacataaag
    33121 cagcttcaga cggccttggg caagcttgtc ggcaatctcg cgcgcctcgt ctgccgatgt
    33181 gccgctgttg actgccgccg cttcaatccc ggccacatgc aggctggcca cttggtcgtt
    33241 catcagcgca atcagcggcg atacgacaac cgccacgcct tcgcgcatca gcgccggaat
    33301 ctggtaacac aaagacttgc ccccgcccgt cggcatcagc accgtcaaac tgccgccgcc
    33361 tgccaaagta ttgatgacat cctcctgcct gccgcgaaat tcgggatagc cgaatacttc
    33421 gtgcaaaatc tgtctggcgg tgggtcggtg cgtcattgtt ccgtgtccgg taagggtgtt
    33481 ggtcggtcgg cggcaatatg ccgtctgaaa tcgggatttt agaagagttt gcccacttcc
    33541 gccccaatat cgtcggcacg cataaacgtt tcgccgatca ggaaggtatg cacgccgcgc
    33601 gcccgcataa attccacatc cgccttgcct gtaatgccgc tttcggtaac gacggttttg
    33661 ccttccagcg cgggcagcag cgacagggtt tggtcgaggg agacttcaaa agtccgcagg
    33721 ttgcggttgt ttacgccccg caacggcgtg gtcaggttgc ggcatttttc caattcggtt
    33781 tcgtcgtgca gctcgagcag gacggtcatg cccaattcgt gcgccagcgc ctcaaagcgt
    33841 tccaattgtc cctgttccag tgcggcggca atcagcagga cggcatccgc cccccatgcg
    33901 cgcgcctgat aaacctggta ttcgtcgatg atgaagtctt tgcgcagcac gggcagcaat
    33961 acggcttcgc gcgcctgttt gaggtattcg ggcgaacctt ggaaataggg ttcgtcggtc
    34021 agtaccgaca aacacgccgc cccggcgttt tcataggcgc gggcaatctc ggcagggcgg
    34081 aagtcggggc ggattaaccc tttgctcggg cttgcctttt tgatttcggc aatgacggcg
    34141 ggcaggttta ggcggtgttt gccgcgtatc gaatcgatga agctgcggac gggcgcggct
    34201 tctgcggcaa gtgcgcggat gtgttcggcg ttgacggcgg ctttttgggc ggcaacttcc
    34261 tgtgccttgg tggcaaggat tttattgagg atgtcggtca tgtcgtgttc cgtatttgtc
    34321 cggggaaagg gggaatatta gcatcaaacc gttaatgcct gtttgtgcgg aagctgtcaa
    34381 aataggacag gacggtctgc ggcagccatt gcaggtgcag cctgccgccg gtgctgctga
    34441 caaagccggc gtgcccgccg tgtgcaggtt ggaacagggt aacggcttcg gacgcttcgt
    34501 ccgcacgggg cagggcttcg ggcggcagga aggggtcgtt ggcggcattg agcaggagca
    34561 gcggtttggc aacgtgtttg agcagcggtt tgcaggaagt ttggcggtag tagtcgtgcc
    34621 ggtcggcaaa gccgtgcagc ggtgcggtga aacggtcgtc aaactcgccc agtgttttgc
    34681 accctgcggc aaatgccgtc tgaaaacctt ggagcgaacg tgctttgggt atcagtgtgc
    34741 ggaggaagta gcgcgtgtag agcagccgcg tgatgccgct gtcgaagcgg ctgcctgccg
    34801 cctctgcatc aacgggggcg gatacggcgg ccgaggcgtg cggcaatgcc tttttgccct
    34861 gttcgcccaa atattttgcc ggcgcgttgc cgcccagcga tacgccgacg gcgtatattt
    34921 cacggtaacg cgcggcgagc gtgtccaaag caaaggcgat ttcggcggta tcacccaagt
    34981 ggtagaacac cggggcggtg ttcgctacgc cgccgcagct gcggaaatgg acgactgcgc
    35041 cgtgccaacc ccgattgcgt accgcgagca tcagttcggc cgcgtaatgg ctgcggctgc
    35101 ttccttccaa accgtgaaac agcacgacca gcggcgcatc gggcgaaatg ccgcctgaaa
    35161 agtcgtaggc ggttttggtt ttacccgtgc tgtcgggaag catctcgcgg cggtatgcgg
    35221 gtgcggggtg ttgcaggaat ttggcggcaa tcgtgtcggc attgccgttg cggaggaaaa
    35281 agggcgtgtc cggcggtgtc aaaatcataa ggtatcggtt tttctgtttt cagacggcat
    35341 tggtgatgcg gcagcccgtc cggccggtgc ggacgcgggc gatgcgcgcc cggatatagg
    35401 cgcggaaaaa gcgttttccg aaaaaggata tcggcatcgg tcagctttcc acgcgtttga
    35461 aatggcgcgg acggaagccc aaagccgcca gagatgcgaa atacagtccg ccgccgacgg
    35521 caatcaggat gcagagctgc cccgctttcc gcattccgcc ggcgtgcgcc cattcgaacg
    35581 gcaggcaagc ctgcgccgcc cacagtccgc cgcacatcac ggcgagcgcg agcagcattt
    35641 tcgccaagaa cgccgcccaa cccctgccgg gccggtaaat accgtgtttg cgcaacagga
    35701 agaacaacaa tccggcgttg atgcacgcgc ccaggccgat ggcgagcgaa agcccggcgt
    35761 gtttcaacgg accgataaag gcgaggttca tcaactgcgt gcagatgagc gtgaagatgg
    35821 cgattttgac gggcgttttg atgttttgcc gcgcataaaa gccggatgcc aacactttga
    35881 tcataattaa accgattaaa ccgaaagaat aggcaatcag cgcgtgttgc gtcatttgtg
    35941 cgtcaaacag cgtgaattct cggtacataa acagcgtcgc caccagcggg aacgacaata
    36001 ccgccagtcc ggccgccgcc ggcagcgtca gcagcatgca caggcgcaaa ccccagtcga
    36061 gcagggcgga aaactgttcc gtatcttggt ttgccgagtg tttggacaaa gtcggcagca
    36121 aaattgtacc gagtgcagcc cccagcacgc cgccgggcag ctccatcatg cggtcggcgt
    36181 aatacatcca tgaaacgctg cccgattgca gataagacgc gaaaatcgtg ttgataacca
    36241 aagaaatttg cgccacgctc acgcccaaaa tcgcaggcgc catctgtttc atgacgcggt
    36301 tgaccgccgc atctttgaaa ttcagtttgg gcagtttcaa aaagcccagt ttcgccagcc
    36361 acggcagttg gaaaccgagc tgcaaaatac cgccgacaaa aaccgcccac gccagcgcgg
    36421 taacgggcgg atcgaaatac ggcacgaaaa acagtgcgaa tacgataaaa gagatgttta
    36481 aaaacgtggg cgtaaacgcg ggaatgccga acttatggta ggaattgagt atcgagccga
    36541 caaaagaaga caaagaaatc aataatatat aaggaaacgt aatccgcagc aggctgatgg
    36601 aaagttggaa cttgtccgcg tctttggtaa agccgggcgc ggaaacataa atcacccaag
    36661 gcgcggcaag tatgcccagc gcggtaacga cgatcagcac aaacgacagc attcccgcaa
    36721 cgtggcggat aaaagcctcc gtcgcctctt tagaacgcgt ttccttatat tccgccaaaa
    36781 tcggcacaaa cgcttgggca aacgccccct ccgcaaacac gcggcgaagc aggttgggca
    36841 gtttgaacgc gacaaaaaac gcatccgtcg ccatacccgc gccgaatgcc cgcgcaatga
    36901 ccgtatcgcg cacaaatccc aaaacgcgcg acaccatcgt caggctgccg acttttgcca
    36961 aagctccaag catattcatc gtgtttcctt ttcggttgaa accccgccct ttagggcggc
    37021 ag
//
LOCUS       DAXDXW010000012        36395 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXDXW010000012 DAXDXW010000000
VERSION     DAXDXW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 36395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36395
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.012"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..1068)
                     /gene="leuB"
                     /locus_tag="ACFB64_000504"
     CDS             complement(<1..1068)
                     /gene="leuB"
                     /locus_tag="ACFB64_000504"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC1208928.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKFIAQGLDADYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEHEGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTGD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            complement(1102..1834)
                     /locus_tag="ACFB64_000505"
                     /pseudo
     CDS             complement(1102..1834)
                     /locus_tag="ACFB64_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
     gene            complement(1850..3121)
                     /locus_tag="ACFB64_000506"
     CDS             complement(1850..3121)
                     /locus_tag="ACFB64_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1208929.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETLEALGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            complement(3288..3929)
                     /gene="leuD"
                     /locus_tag="ACFB64_000507"
     CDS             complement(3288..3929)
                     /gene="leuD"
                     /locus_tag="ACFB64_000507"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC1208930.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNGKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAIIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADKIKAFEEKRRQSQPWLFNG"
     gene            complement(3991..4245)
                     /locus_tag="ACFB64_000508"
     CDS             complement(3991..4245)
                     /locus_tag="ACFB64_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208931.1"
                     /translation="MNKLFVTALSALALSACAGTWQGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGKKIENATE"
     gene            complement(4340..5749)
                     /gene="leuC"
                     /locus_tag="ACFB64_000509"
     CDS             complement(4340..5749)
                     /gene="leuC"
                     /locus_tag="ACFB64_000509"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC1208932.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDQNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKGKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLNIGGKVPNPAEETDPVKRSGIERALEYMGLKAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKGHKKAGNVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPRQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGHFTDIRTMA"
     gene            6001..7350
                     /gene="gshA"
                     /locus_tag="ACFB64_000510"
     CDS             6001..7350
                     /gene="gshA"
                     /locus_tag="ACFB64_000510"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFC1208933.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWNAHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETEKSVLIIPE
                     NHTRNTFYLQNVYALGEIFRSAGCEVRLGSLNPEVTEPAEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGIGQTVLPPLHGGWTTRRKTNHFGAYNQV
                     AAEFAKLIDIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGIAD
                     KPFVIVKADAGTYGMGVMSVKSSDEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGVYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            complement(7324..7446)
                     /locus_tag="ACFB64_000511"
     CDS             complement(7324..7446)
                     /locus_tag="ACFB64_000511"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208934.1"
                     /translation="MPSETDVPTRQISEMPSEPDFSFRRHLYFKTVLRRFFQLD"
     gene            7473..8150
                     /gene="radC"
                     /locus_tag="ACFB64_000512"
     CDS             7473..8150
                     /gene="radC"
                     /locus_tag="ACFB64_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC1208935.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILEEELQEEIT
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQTVRSF
                     RQLGLMP"
     gene            8223..8948
                     /locus_tag="ACFB64_000513"
     CDS             8223..8948
                     /locus_tag="ACFB64_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC1208936.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENMRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVAYWDFNWRKGGGGARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPV
                     KLGVLYYGDESTQYEPAPVRVDK"
     gene            9100..10380
                     /locus_tag="ACFB64_000514"
     CDS             9100..10380
                     /locus_tag="ACFB64_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC1208937.1"
                     /translation="MKSLFIWLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRTQIGLHALAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAVYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGSFERACAKGRRQPEAGRKYYRNACHNGAAVYADEAMP
                     VTELLYTAYPVGGGALPYFYGERPDPVPEYEITGNPASIDFSEAAGKIAMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAHQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRRQFGNSLSILRHE
                     AGGIVFSVSGMAGSRIRLTPEDSPERGVTLYLQD"
     gene            10386..11564
                     /gene="hflX"
                     /locus_tag="ACFB64_000515"
     CDS             10386..11564
                     /gene="hflX"
                     /locus_tag="ACFB64_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC1208938.1"
                     /translation="MSGRTGRNSATQAQPERVMLVGVMLDKDDTGSNAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLTAHRINALKKQLANLKKQRALRRKSRESGRIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPACSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAHDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAEYCAAAPNTDETEMP
                     "
     gene            11561..14032
                     /locus_tag="ACFB64_000516"
     CDS             11561..14032
                     /locus_tag="ACFB64_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC1208939.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCVGCQAVAQSIIDS
                     GLGSYYKRRTADAKKTELPPQEILDQIRLYDLPEVQSDFVETHNGTHEAVLMLSGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQTGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAAAIIMTFIAGIYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVRESAVVKLQ
                     AGDIVMVKPGETIPVDGTVLEGNSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLSQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPEIQVGQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLQDPLKDSAAEAVRQLAGKNLTLHILSGDREEAVAETARALGIAHYRA
                     QAMPEDKLEYVEALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKILSEQ"
     gene            complement(14691..15467)
                     /locus_tag="ACFB64_000517"
     CDS             complement(14691..15467)
                     /locus_tag="ACFB64_000517"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC1208940.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCIRDESLRFENGQFVMVGLMA
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDEVLISKKPTGTLVAC
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHRGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(15655..16449)
                     /locus_tag="ACFB64_000518"
     CDS             complement(15655..16449)
                     /locus_tag="ACFB64_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208941.1"
                     /translation="MTLPMQETRFSILLDELAAKQEATIAPYLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKRQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAGTVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            complement(16496..17905)
                     /gene="thrC"
                     /locus_tag="ACFB64_000519"
     CDS             complement(16496..17905)
                     /gene="thrC"
                     /locus_tag="ACFB64_000519"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC1208942.1"
                     /translation="MRYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMCLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     CLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIRQVYEQDKELIDPH
                     TANGVKVAREVREAGETVVCLETALAAKFDATIREAVGDVAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            complement(17978..18346)
                     /locus_tag="ACFB64_000520"
     CDS             complement(17978..18346)
                     /locus_tag="ACFB64_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208943.1"
                     /translation="MNKTLSILPAAILLGGCAAGGNTFGSLDGGTGMGGSIVKMTVES
                     QCRAELDRRSEWRLTALAMSAEKQAEWENKICGCATEEAPNQLTGNDVMQMLNQSTRN
                     QALAALTVKTVSACFKRLYR"
     gene            complement(18597..20061)
                     /locus_tag="ACFB64_000521"
                     /pseudo
     CDS             complement(18597..20061)
                     /locus_tag="ACFB64_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
     gene            20316..21232
                     /locus_tag="ACFB64_000522"
                     /pseudo
     CDS             20316..21232
                     /locus_tag="ACFB64_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(21253..21447)
                     /locus_tag="ACFB64_000523"
                     /pseudo
     CDS             complement(21253..21447)
                     /locus_tag="ACFB64_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063514830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(21760..23364)
                     /locus_tag="ACFB64_000524"
     CDS             complement(21760..23364)
                     /locus_tag="ACFB64_000524"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC1208944.1"
                     /translation="MNYKDDTRITGATVSDKGLVAIKPLNNTNIVADTIHYKGDVLAV
                     NKGKVELDFTPNIRLVGRLDNFSGLTDSKHKNLFENYVANLDSKSAGEINFNLAKDAL
                     WTMTGQSWLDKLEGQGTIDFNNDAKTSGRALHIGELAGANKFLMHLNKDGIHSDMLYV
                     KKGTSTPQEVVVKNLSEVLDSMNYGERLRFATVTNSKNEFVNGKKYIDDTHLMEDALT
                     VEYSAHNGDKNNKDDYNKSFNGSEMTAEKAGDDYVNKTYTDNRQNVYLVKQATGNPSR
                     NVKNINDMFDSTAHYAFTLDTYAKREGERAFSTLDKKEGDWIRLTHTRVIQSNAFRFH
                     NNDFEIGYDRFSLNEQEKKRKWGISLDYGHGRTSLWNTFGKDKIRKYELALYNTTQYI
                     DKEGDETGYIDNVLKIGKLRNRVIARNHMGQLWGKGKYSNTLFSISTEYGRRKFLDDD
                     KLWRITPQVQLQYSYLRGTGYRIDNGINVNLSHANSLIGRLGLDVVRKFDGGKKLFYI
                     KGNIFHEFLGSRSFKAFEGKSHYAQK"
     gene            complement(23482..24009)
                     /locus_tag="ACFB64_000525"
     CDS             complement(23482..24009)
                     /locus_tag="ACFB64_000525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208945.1"
                     /translation="MNQEGITAHGNATITLKAKENNKITVENAAYSSDGISTLINRTG
                     ARPGTRDDGNKIILEAGGDNIVTMKSGDADADYVNNSKVLTETPYYKSKRGSNGIFAY
                     GDKSLVKLIGENNIVKSEISEKSKALNGGFRHIGIYSWQNAKVELSAKSDNIVQGGIW
                     GLYSNNSSISLKGKK"
     gene            complement(24815..25116)
                     /locus_tag="ACFB64_000526"
                     /pseudo
     CDS             complement(24815..25116)
                     /locus_tag="ACFB64_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714068.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25313..26623
                     /locus_tag="ACFB64_000527"
     CDS             25313..26623
                     /locus_tag="ACFB64_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC1208946.1"
                     /translation="MTDLFVREPDAPLAERLRPHTLDDVIGQQHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIRGAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     SLSSDDLKKLIAKVLALPEYRDFTIEADVQELLVNTADGDARRLLNLLEQLLRAADTR
                     RLKILTTEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            26743..29748
                     /locus_tag="ACFB64_000528"
     CDS             26743..29748
                     /locus_tag="ACFB64_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFC1208947.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNANWDRQTNIFPEI
                     FVDSLCRINPDLPPDDARRLLADIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEAFRPDIALLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     PKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSACGKPVFNYFREEHKLNLTEL
                     LDTLSDDLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLAFLLQHGL
                     TYVKASQGSVQKHIMRYPQLFATLAIEKHLSNGGKKGVIWHTQGSGKTAPAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAQREFTARDLIVHTIDSREAFAADIKSAQTLHN
                     HAGKPEITVVNIQKFQDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREEIGSRYK
                     AQLQEALAQLEIEKGSFDRKEIYAHPHFVHPMLDYILDDFAKFRKTNQDESLGAMVVC
                     DSAEQARQLFEHFQTASDHNFTAALILHDVGTKEERDQWVKDFKAGKTDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKTNRAY
                     WDELSNELGDEIGSYSQLFKTAEEIEQEIADIKNALFDFDTENAEEFCSQISQIEDKK
                     QLLALKKALQTAKELYNILRLQGSHEFLAHLDFDKLNLLYRETAARLDTLNLAEKLQQ
                     GDTAHLLNEALEDVYFQFVKIGEAELKLADDLKDIMRKVREGLAGNFDQDDLEYISLR
                     EELERIFKKKNLAEVGQEEMQANIATLQTVYTKIKEPNRQNDLLRHKYGGDAKYARIH
                     KRLMENAALYGDKLKVFNALNGVKTDADQRVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPADIQTINRLLVREYLKESGRI"
     gene            29797..30027
                     /locus_tag="ACFB64_000529"
                     /pseudo
     CDS             29797..30027
                     /locus_tag="ACFB64_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            30077..31279
                     /locus_tag="ACFB64_000530"
     CDS             30077..31279
                     /locus_tag="ACFB64_000530"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC1208948.1"
                     /translation="MNHFKKQQIQNIADFNPREQLAKGALAKSVPMAMLKEFQRQITG
                     YEIKAFNGGAKFRNGDTLLAKITPCLENGKTAFVDILDDGEVAFGSTEFIVLRAKNET
                     NPEFLYYFAISPDFRKRAIECMEGTSGRQRVNENALKTLELPIPEPQIQQSIAAVLSA
                     LDKKIALNKQINARLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGDMVFDETLKREIP
                     KGWGSIELQSCLAKIPNTTKILNKDIKDFGKYPVVDQSQDFICGFTNDEKSILNPQDA
                     HIIFGDHTRIVKLVNFQYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYARHF
                     KFLKEFKIILPSKDISQKYNEIANTFFVKVRNNLKQNHHLTQLRDFLLPMLMNGQVSV
                     RCSGARDG"
     gene            31272..31832
                     /locus_tag="ACFB64_000531"
     CDS             31272..31832
                     /locus_tag="ACFB64_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208949.1"
                     /translation="MDKLFKILLNISSTSLLLSIYFIKSQYYILDLTWFYRVISNHWI
                     EPISKLSLLFYFLIPFIATATVLWLSKYLGKDEFKQGEVKELEYVNDNFLPSYLGYFF
                     VALSIPDNNLFLLFVMYGIIFLLVSCSKSFYFNPVFFLFGYRFYQAKTESGLLLVLIS
                     KQEFRTPQSVSSIAVYRINNFTFLEK"
     gene            31843..32682
                     /locus_tag="ACFB64_000532"
     CDS             31843..32682
                     /locus_tag="ACFB64_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208950.1"
                     /translation="MNFVLAKITCKKDLVKILSDDHIFPDFSYENLNFITYNYDYNLD
                     DDTWFQIENLKNQDFCPKFLDNSNLFDSKMFSEIKKEEINIEKLKYLVSCTNDALFFQ
                     KITSSLLLKKKHLLTICGNGAKLCEPQDLLVIKDIPDAVYIIKDDKLIFRTLSSISNI
                     FKGIEDLYREATNTEVQQFLESDFIDLKEDFLSEKVSIPNRKRIALVQDRLNNMTLDQ
                     RQELLNYLAEYNNILKFNADGSRVEISTDVQLKHLLYGIDERYYTTALGKEKRLANSV
                     QPI"
     gene            32749..34350
                     /locus_tag="ACFB64_000533"
     CDS             32749..34350
                     /locus_tag="ACFB64_000533"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC1208951.1"
                     /translation="MTEQHFTEQIKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKKIRKEKPDEPIEFVNMDIDGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAACNADLFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPEEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHVIGEDKCMIYTQDISQKSSNLLRLNLSLNNLVHSLNNVVQG
                     NTILSPAHKDASGCLKKFDFIVSNPPFKLDFSDFRDRLESDENHERFFAGIPKIKPTK
                     KEKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAKSGIDKKIREYLVENKMLAGVVS
                     MPSNIFATTGTNVSILFIDKVNKDKVVLIDASGLGEKISINDNQKTVLSHEEEQKICH
                     TFTHKQAVEDFSVVVGYDEIKAKNHSLSAGQYFEVKIDYVDISADEFVQKIAGFSADL
                     DKLFAESAELEKEIKDRLAMLKFNS"
     gene            34382..34498
                     /locus_tag="ACFB64_000534"
     CDS             34382..34498
                     /locus_tag="ACFB64_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC1208952.1"
                     /translation="MPSESLSDGILFGLENTYAVLSPKERIQKTKATGIHRK"
     gene            complement(34661..35998)
                     /gene="ribBA"
                     /locus_tag="ACFB64_000535"
     CDS             complement(34661..35998)
                     /gene="ribBA"
                     /locus_tag="ACFB64_000535"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC1208953.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQNRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTGRPPGHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAVNFMIKHARGLVCLPMEGAMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPADIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGDAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHHTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLHVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
BASE COUNT         9190 a         9063 c         8956 g         9186 t
ORIGIN      
        1 caaggcggcg agtaccgcgt cgcccatttc ggagcaggaa acgagttttg tgccttcttc
       61 gtaaatatcg ccggtacgca agccttgttg cagcactttt tggacggcgt tttcgacttg
      121 ttgcgcgcgc gcttcgtcgt tcaggctgta acgcagcaac atggcaagcg agaggatggt
      181 ggccagcggg ttggctttgt tttgtccggc gatgtcgggg gcggagccgt gagacggttc
      241 gtacaggcct ttgccgtttt cgtccaaaga agcggaaggc agcataccga tggagccggt
      301 cagcatggag gcttcgtcgg agaggatgtc gccgaagatg ttgccggtgg caatcacgtc
      361 aaactgtttg ggcgcgcgca cgagttgcat ggcggcattg tccacataca tatgggaaag
      421 ctcgacatcg ggatattgtt tgccgatttc ttcaaagatt tcgcgccaca gttcggtggt
      481 ttccaaaacg ttggctttgc ctacggagca gacttttttg ctgcgttttt gggcggattg
      541 gaaggccacg tgggcgatgc ggcggatttc gctttcgctg tatttcatgg tgttgtagcc
      601 ttcgtgttcg ccgttttcca aaacacggat gccgcgcggt tcgccgaaat aaatatcgcc
      661 ggtaagttcg cgcacaatca aaatatccaa accggcaacg atttcaggct tgagcgtgga
      721 ggcgttggct aattcgggat ataaaacggc aggacgcaaa ttggcaaaca ggttcaaatc
      781 cttacgaatt gctaacaagc cgcgctcggg gcgcaacgga cggtcgagat tgtcgtattg
      841 gggcgaaccg actgcaccaa gcaggacggc atcggctttg cggcagaggt tttgcgtaaa
      901 ttctggataa ggatggccgt attcgtcgta ggcttcgccg cccaaaggcg cgtattcgta
      961 gtcggcatcc aagccttggg cgataaattt gtcgagtacg cggacggttt cggcgacgat
     1021 ttcgggaccg atgccgtcgc ctcggaggat ggcgatatgt ttggtcattt caagtttcct
     1081 tatgggttga tggttgaagg gttatttctt tttgtatttg tgtgcaattt cgtgccaacg
     1141 gggtatggaa atcgatcggt tgtagtgttt tttataagct tcctcaaatt tctttttcca
     1201 taaggatgcg ttatgccgtg ttgccgggtt ttgataaacg gtttcttcaa ttgcggaaac
     1261 aaaatcttcc aagcattcaa acataggcat attcttttta tttatgctgt accaaacacc
     1321 cgggcggatt ttatgcacca cgcctttttt gacttgaaga ttaattgccc acccatccat
     1381 gcttctggta atttgccata cttttttaag ccataaatcc ttgatggtta cattgccgtt
     1441 gtcgtccata tcgtaaccga atattaaata atctacatcc agcatatagg gcttatgaat
     1501 gatttcatca gaatacattt taaaatctgc aatatcaaaa cccgggcagg catttcggtt
     1561 gaacgccttt acttccaaca attctctgct gcggcctttt ttatttaaaa aaaaaatcgg
     1621 gaggcatttg ggtattggtt gaaacatcaa attcaatttc cctttttctc aaccatccgc
     1681 cgagccattc ctgaatgatg ttgccgacaa catctttttg tttgacgata atatccacat
     1741 cgcctaagaa aaatctaatt tgaccattgg ccgataagat tttttcttca tccagcaact
     1801 tatcaaatat ttgttgtgca gtaagtttta tcattttatc cctgtcggtt tataaagtat
     1861 gcagaagtct ttcagatacc gccttaatca cagggacggc aacggtatta cccaataaat
     1921 cgcatctgtc ttttttggga atatcaaacg aataatcgtc cggatagccg aataagcgta
     1981 acccttcttt tccggtaagt gtacgcaaac cgccgttgtc aacgacgaaa aggtgctcca
     2041 tatccattgc aaccaaggtt ggcgcaacat cgtttggatc taatatttta ttgatttcaa
     2101 aagatttttt acctgaaaca atattgtagc ctttgggcag ggattcatct ttgattcttt
     2161 gcccgccaat tttttgtttc ggatgctcta aaaccaaata gcctttgtct gtcaggctgt
     2221 ccaaaatatt ttggagatcg gggtgtttat agaaagttga aatttgcgct tttgtcaaag
     2281 gcatcccatc catccaatct atgccgattt ctgaagccca tttttttttc ctccgttctt
     2341 ttagaaggat atttaacaat tgcttctctt cttcggttac tgcgcctttt aattcaatat
     2401 cccaactgtg aatattgttt ttccctcccc gtttgtcttt tactgatttt ccgtacagtt
     2461 cggacggggg aaattttttt agcaattttt tgatgaaagg gctgctttca gtaggcagtc
     2521 ccgattccaa aatatttttt aatttcggac ttgggcttgt ttcgaaagat aagtcgggtt
     2581 tggatttcaa actgcctgtc agataaatgc gcttcctgtt ttggggaatg ccgaaatctt
     2641 ttgcatttaa aactttccaa gaaacatagt agcccaatgc ttccaaggtt tccaaaataa
     2701 cggtcagggt gcgccctatt ttttgtgtcg aatcttttct atcgtgtgtc accaatcctt
     2761 ccacattttc caaaataaaa ccttttggtt tttttgcctt taaaatcctt gccacatcaa
     2821 agaaaagcgt tccccgtgta tcttcaaagc ctaatctttt tccggcgaaa gaaaaagcct
     2881 gacaagggaa gcctgccaac aggatgtcaa aatcgggaat atcccctgtt tcaattttcg
     2941 ttatatctcc atacggcact tcatcagggt agttttgctt caatacttcc aaagctgccg
     3001 gtttgatttc tgaagtaaaa acacattcgc aagcaaccga ctgtttccga caggcttgtt
     3061 cgaatccttt cctgatgcca ctcattccgg aaaataagtc aataaattta atttgttgca
     3121 tattaaaaat ctaaaaattt atttgaaatg gagagttgca ttaatttaga gtgttgctaa
     3181 gcccgcttaa aagatgaaaa caatttatcg cccctctgtt tacattagcc gcagcaatta
     3241 tatgttatca ggaatgccgt ctgaacagcc ttcagacggc ataggtttta accgttaaac
     3301 agccaaggct ggctttggcg gcgtttttct tcaaaggctt taattttgtc ggcgtgttgc
     3361 agggtcagtc cgatttcgtc caagccgttt aagaggcagt gtttgcggtg ttcggtaatg
     3421 tcgaatgtga atgtttcgcc gcccggggtg gtcagggttt gctcggcaag gtcgatggag
     3481 agccgatagc cttcgttggc ttcgacttct ttgaaaagcc ggtcgacttg ttcttcggtc
     3541 aacacgatgg gcaaaaggcc gtttttgtag cagttgttaa agaagatgtc ggcgaagctg
     3601 ggggcgataa tggcgcggaa gccgtagtcg tccaatgccc aaggggcatg ttcgcgtgaa
     3661 gagccgcaac cgaagttttt acgcgtcaac aggatttgcg cgccttggta acgcggctgg
     3721 ttcagggaaa aatcggggtt caacgggcgt ttgccgttgt ccatgcccgg ttcgccgtgg
     3781 tcgaggtaac gccattcgtc aaaggcgttg gggccgaagc cgctgcgttt gatggatttc
     3841 agaaattgtt tggggatgat ggcatcggta tcgacgttgc tgcggtcgag cggggcgacg
     3901 atggcggtga ttttggtaaa ggctttcatg ggtttgcgtc ttgtgctgac gatgccgtct
     3961 gaaacggttt cagacggcat cgcaaatcgg ttattcggtg gcgttttcga tttttttgcc
     4021 gagatgggaa atgccgcgtc cgacggcatt gccgcctttt ttgacggctt ctttggtttt
     4081 gtcccaacct ttttcgacgg cgttgcctgt ttgttcggcg gcgcgttcgg cggcggcctg
     4141 tgttttgtca aggttgcggg cggtgtcttg tttcgcgccc tgccaagtgc cggcgcaggc
     4201 ggacaaggcg agggcggaca gggcggtaac gaaaagtttg ttcatggtta aactccttgg
     4261 tttgaatatt aggtgtttct gccttacggg acatatttca gacggcctcg tcaaattctt
     4321 aaagaccgcc tgaaaatact tacgccatcg tgcggatgtc ggtaaagtgt ccggtaacgg
     4381 cggcggcggc ggccatagcg gggctgacga ggtgggtgcg cccgccgttg ccttggcggc
     4441 cttcaaaatt gcggttggac gtggaggcgc agcgttgcct cggcgcgagg cggtcggcat
     4501 tcatcgcaag gcacatcgaa cagcccggtt cgcgccattc aaaaccggct tcgatgaaaa
     4561 ttttgtccaa gccttctttt tcggcttgtt ctttaaccaa accggagccg gggacgatta
     4621 acacgcgctg tacgttgccg gcttttttat ggcctttggc gatagcagcg gcttcgcgca
     4681 agtcttcgat gcggctgttg gtgcaagagc cgataaagac gatatcgaca gggatttcgt
     4741 tcagcggcgt accggccttc aaacccatat attcgagtgc gcgctctatg ccgctgcgtt
     4801 tgaccggatc ggtttcttcg gcgggattcg gcactttgcc gccgatgttt aaaaccattt
     4861 cgggcgacgt gccccaagta acttgcggtt cgatgtcttc ggcgttgaaa cggtattctt
     4921 tatcaaaaac cgcgccttcg tcagacacca gcgtgcgcca gtactcgacg gctttgtccc
     4981 aagcttcgcc ttcgggtgcg aagggcttgc ctttgacata gtcgatggtg gtttgatcga
     5041 cggcaaccat accggaacgc gcgcctgcct caatcgccat attgcacagg gtcatcctgc
     5101 cttccatcga aaggctgcgg atggcttcgc cgccgaactc gacggcgtaa cccgtgccgc
     5161 ccgccgtgcc gatttgcccg atgatgtaga gcgccacgtc tttggcggta acgcccgctt
     5221 ttaatttgcc gtcaacggca atcagcatag atttggattt tttcgcggta atgcactggg
     5281 tcgccatcgt gtgttcgact tcggaagtgc cgatgccgtg tgccagtgcg ccgaatgcgc
     5341 cgtgggtgga agtgtgcgaa tcgccgcaga cgacggtcat cccgggcagg gtcgcgcctt
     5401 gttcgggccc cataacgtgt acgatgccct ggcctttgtc cataaacggg aagtaggcga
     5461 gtgcgccaaa ctctttgatg ttttgatcca acgtatcgac ttgcagcttg gaaatcgggt
     5521 cttggatgcc tttgtcccaa tcgccggtcg gggtgttgtg gtcggcggtg gagaccacgc
     5581 tgtcgatgcg ccacagcttg cgccccgcca ttttcagacc ttcaaacgcc tgcgggctgg
     5641 ttacttcgtg aaccagatgg cggtcgatgt aaagcaggac ggtgccgtct tcttcttcgc
     5701 ggacgacgtg gctgttccag agtttgtcgt agagggtttg tgccgtcatg atgttgttct
     5761 tttggataaa tggtgatgcg gattgggcgg attttagacg tattctttat actgcgcaac
     5821 agattttgtc taatttttga gtcggtgtta ttttgtaaac aattttaacg aaaaaattag
     5881 acatattgtt catttcgtta tatctaaaga atgattcgat acgaaagaat acttgtcgtc
     5941 attctttcaa aagcattatt atctgtacct tgtcaaaaaa cacacagagg cagacgaaag
     6001 atgaaattac cggttatgtc gcccgaacat tcggcgcaac ttcaggcgtt tgaggcaaaa
     6061 atcctgtcca atcacgccaa aatcgaggcg tggttccgca cgcagtggaa cgcgcaccgc
     6121 ccgccgtttt acggttcggt cgatatacgc aacgccggtt acaaaatttc gtctatcgat
     6181 atgaatttgt ttccgggcgg cttcaataat ctgaatccga atttcatccc gctggcggcg
     6241 gttgccgcgc aagatgcggt gcaacgcgcc tgcgaaacgg agaaatccgt attgattatt
     6301 cccgaaaacc acacgcgcaa tacgttttac ctgcaaaacg tttacgccct cggcgagatt
     6361 ttccgttcgg cagggtgtga agtgcgcttg ggcagcctga atccggaagt aaccgaaccg
     6421 gccgaatttg aaactgcatt gggcgacaaa atcctgcttg agcctttgct gcgaacccgc
     6481 gaccgcgtcc accttgccga cggcttttcg ccttgcgtgg ttttgttgaa caacgatttg
     6541 tccgccggca ttcccgacat tctcaaaggc atcggccaaa ccgttttgcc gccgctgcac
     6601 ggcggttgga cgacgcgccg caaaacaaat catttcggcg cgtacaacca agttgccgcc
     6661 gaatttgcca agctgattga catcgatgaa tggcagatca acccgtattt tgaaaaaatc
     6721 ggcggtttgg acttccaagg gcgcgaaggt gaagatgcgc tggcggaagc ggtagaacgc
     6781 gtgctggcga aaattcaagc caaatacgac gaatcgggca ttgccgacaa acctttcgtc
     6841 atcgtcaaag ccgatgccgg cacttacggc atgggtgtga tgagcgtcaa atcctccgac
     6901 gaagtgcgcg gcttgaaccg caaaaaccgc aacaaaatgg cgaaggtgaa agagggcttg
     6961 gaagtcagcg aagtgattgt ccaagaaggg gtttatacct atgaaacctt gaacggcgcg
     7021 gtgtgcgaac ccgtcgtcta tatgatggac cgtttcgtca tcggcggctt tttccgcgta
     7081 cacgaagggc gcggtgcgga cgaaaacctc aacgccggcg gtatggtgtt tgtgccgctg
     7141 tccaacagca ttcctaccgg taacggcgat aattcgcaag aagctccaga agcctgcaaa
     7201 cgcgtattcg aacaatggga ttcgctcggt atgccgcgtt ctgaaaaaga ctgcgacgtg
     7261 gacaacgaac ataaccgcct ctacgtttac ggcgtaatgg cacgtctgtc gcttttggcg
     7321 gcttcaatcg agttggaaga aacggcgtaa gactgttttg aaatacagat gccgtctgaa
     7381 gctgaaatcc ggttcagacg gcatttcgga tatttggcgt gtgggaacat ctgtttcaga
     7441 cggcatctca gactatttaa aaaagggaaa acatgagcat caagcaatgg ccggaaggcg
     7501 aaaggcccag ggaaaagctg ttggaacgcg gggcggcggc tttgagcgat gccgaacttt
     7561 tggcaatcct tttacgcgtc gggacgcgcg ggatgagcgc ggtcgatttg gcgcgctacc
     7621 tattgcagga gttcggcagc ttggggaggc tgatgagcgc ggaggtcggc aaactgtcgg
     7681 catacaaagg gatggggacg gcaagtttca cacagttcgc cgtggtcagg gaaatcgggc
     7741 ggcggatatt ggaggaagaa ttgcaggaag aaattacctt atccgatccc gataccgttg
     7801 ccgattattt acgctttcat ttggggcagg aaaaagtcga agtcagcgtc gcgctgctgc
     7861 tgaaccgcca aaaccaactg attgcggtca gagagctgtc gcgcggtacg gttgcggaaa
     7921 acacgattta catccgcgaa atcgtcaaac tggcattgga cgaatatgcc gacagcctga
     7981 ttatcgcgca caaccatccg ggcggctcgc ccgaaccttc gcaggaagac atcatgttca
     8041 caaggcggct ggcacaggcc atgtcgctgg ttgatgtttc gctgctcgac cattttatcg
     8101 ttacctcgca aaccgtccgt tcgttcaggc agctcgggct gatgccctga caccctgttt
     8161 tacattcggc ggctctggta aaatagccgc tttgaccgta ttcgacagat attgttaagt
     8221 taatggaaac acaaaacaaa cctaccgtta ccgacattga ccgccccata ctcgtcccgc
     8281 ccggcggaca taaaaaagtc ttgctgcatt cctgctgcgc cccgtgcagc ggcgaagtga
     8341 tggaagccat gcttgcctcc ggcatcgact acaccattta tttttacaat cccaatatcc
     8401 atccgcacaa agagtatatg ctccgaaaag aggaaaacat gcgctttgcg gaaaagttcg
     8461 gcatcccttt catcgataaa gacgacgact acgaaaacga ccgcaaagaa tggtttgcca
     8521 aagccaaagg catggagttt gagccggaac gcggcatccg ctgcaccatg tgtttcgata
     8581 tgcgttttga aaaagcggcg caatacgcgc acgaacacgg ttttcccgtc tttaccagtt
     8641 cgctgggcat ttcacgctgg aaaaatatgg cgcaaatcaa cgactgcgga caccgtgccg
     8701 ccgcgcctta cgatgatgtg gcctattggg acttcaactg gcgcaaaggc ggcggcggcg
     8761 cgcgtatgat tgaaatcagc aaacgcgaaa acttctacca gcaggaatac tgcggctgcg
     8821 cctattcctt gcgcgattcc aacgcccacc gcaaatcgca gggacgcatc cccgtcaaac
     8881 tcggcgtgct gtattacggc gacgaatcga cacaatacga acctgcaccc gtccgagtgg
     8941 acaaataaac acccgatgcc gtctgaaggt tcagacggca tcgggttcgg caccggcacg
     9001 ggaaaggttt gccggtttgg caatcggcaa gcggaaaccg cattggcaag tttgccgttt
     9061 tgataaaaca ccccgttgcc gcgtcgggag ggcggcatta tgaaatccct ttttatttgg
     9121 ctgcttctat tgggctcggc ggcaggcgtt ttctaccata cccaaaacca atccctgccc
     9181 gcgggcgaac ttgtctatcc gtccgcaccg caaatcaggg acggcggcga tgcgctgcac
     9241 tacctcaacc gcatccgcac acaaatcggt ttgcacgcgc tggcacacgc gccggttttg
     9301 gaaaattccg cccgcaggca cgcacgctat ctcacgctca atcccgaaga cggacacggg
     9361 gaacaccatc ccgacaatcc gcactacacc gcacaaaagc tgaccgaacg cacacgcctt
     9421 gccgggtatc tctacaacgg cgtgcatgaa aacatcagca cggaagagga agccgccgaa
     9481 tcgtccgaca gcgacatccg cacgcagcaa cgccaagtag acggattgat gagtgctgtc
     9541 tatcaccgcc tttcgctgct tgaccgccat accgacgaag caggtgcggc atttgtgcgc
     9601 gaaaacggca aaaccgtcct cgtattcaat cagggcaacg gcagcttcga gcgcgcctgt
     9661 gcaaaaggaa ggcggcagcc ggaagcagga cggaaatatt accgcaacgc ttgccacaac
     9721 ggtgcggccg tttatgctga cgaagccatg cccgtaacgg aattgcttta taccgcctat
     9781 ccggttggcg gcggcgcgct gccttatttt tacggggaac gtcccgaccc cgtgccggaa
     9841 tatgaaatca caggcaatcc tgccagcatt gatttttccg aggcggcagg caaaattgcg
     9901 atgaaaagtt tcaagctgta tcagggtaaa aacgaaatcc gccccgtcag ggttttaacc
     9961 gccggcaacg accctaacgg caggctgacc gcgcaccaat tcgccctttt cccgctcaaa
    10021 cctttggaat acggcacgct ttatacggcg gtattcgact atgtccgcaa cggacggcgc
    10081 gcgcaggcga aatggcagtt tagaacccga aaacccgatt acccttattt tgaggtaaac
    10141 ggcggcgaga cacttgcggt tagaaaaggc gaaaaatatt tcatccactg gcgcggacgc
    10201 tggtgtctgg aagcgtgtac ccgttatacc taccggcggc agttcggcaa cagcctgtcc
    10261 atactccggc acgaagcggg cggcattgtc ttcagcgtca gcggaatggc gggaagccgc
    10321 atcaggctta ctccggaaga cagcccggaa cgcggtgtaa ccctttattt acaggattga
    10381 cagttatgtc aggtagaaca ggacggaaca gtgccactca ggcgcaaccg gaacgcgtca
    10441 tgctggtggg cgtaatgttg gataaagatg atacgggcag caatgccgcc cgtctgaacg
    10501 gttttcagac ggcattggcg gaagccgtcg agctggtcaa ggcggcgggc ggcgattccg
    10561 tacgcgtgga gactgccaaa cgcgaccgcc cgcacactgc gctgtttgtc ggcacgggca
    10621 aggcggcgga gctgtcggaa gcagttgccg cagacggcat tgatttggtc gtattcaacc
    10681 acgaacttac tcccacgcag gaacgcaatt tggaaaaaat cctccaatgc cgcgtattgg
    10741 acagagtggg gctgattctg gcgattttcg cccgccgcgc ccgcacgcag gaaggcaggc
    10801 tgcaagtcga gttggcgcaa ttgagccatt tggcgggacg cttgatacgc ggttacggac
    10861 atttgcaaag ccagcgcggc ggtatcggca tgaaagggcc gggcgaaacc aaactggaaa
    10921 ccgaccgccg attaaccgcc catcggatca acgccttgaa aaaacagctt gccaacctca
    10981 aaaaacagcg cgccctgcgc cgcaagtccc gcgagtcggg cagaatcaaa acgtttgcgc
    11041 tggtcggcta taccaatgtc ggcaaatcca gcctgttcaa ccggctgacc aagtcgggca
    11101 tatatgcgaa agaccagctt ttcgccactc tcgacacgac ggcgcggcgg ctgtacatca
    11161 gtcccgcatg cagcattatc ctgaccgata ccgtcggatt cgtcagcgat ctgccgcaca
    11221 aactgatttc ggcattttcc gccacgctgg aagaaaccgc gcaagccgat gtgctgctgc
    11281 acgtcgtcga tgccgctgcc ccgaacagcg gacagcagat tgaagacgtg gaaaacgtac
    11341 tgcaagaaat ccatgcccac gatattccgt gcatcaaggt gtacaacaaa accgacctgc
    11401 tgccgtctga agaacaaaac acgggcatat ggcgcgacgc tgcgggaaaa attgccgccg
    11461 tccgcatttc cgttgctgaa aataccggta tagacgcact gcgcgaagcc attgccgagt
    11521 attgtgccgc cgcaccaaac acagacgaaa ccgaaatgcc atgaaaaaaa cctgtttcca
    11581 ctgcgggctg gacgttcccg aaaacctgca tctgaccgtc cgctacgaag gcgaagaccg
    11641 cgaaacctgc tgcgtcggct gtcaggcagt cgcacaaagc atcatcgatt ccggcttggg
    11701 cagttattac aaacgacgca ccgccgacgc gaaaaaaacc gaactgccgc cccaagaaat
    11761 cctcgaccaa atccgcctgt acgacctgcc cgaagtccaa tccgactttg tggaaaccca
    11821 taatggcacg cacgaagcag tgctgatgct cagcggcatt acctgcgccg cctgcgtctg
    11881 gctgatcgaa cagcagcttt tgcgcacaga cggcatcgtc cgcatcgacc tcaattacag
    11941 cacgcaccgc tgccgcgtcg tctgggacga cggcaaaatc cgcctctccg acattctgtt
    12001 gaaaatccgg cagacaggct acaccgccgc accctatgac gcgcaaaaaa tcgaagccgc
    12061 caaccaaaaa gaacgcaaac aatacatcgt ccgccttgcc gttgccggac tgggtatgat
    12121 gcagacgatg atgttcgcgc tgccgaccta cctttacggc ggcgacatcg aacccgattt
    12181 cctgcagatc ctccattggg gcggcttcct gatggtgctg cccgtcgtat tttattgcgc
    12241 cgtcccgttt tatcaaggcg cgttgcgcga cctgaaaaac cgccgcgccg gtatggacac
    12301 gccgattgcc gccgccatca tcatgacctt catcgccggc atttacagcc tcgccaccaa
    12361 cgcagggcag gggatgtatt tcgaatccat cgcgatgctg ctgtttttcc tgctgggcgg
    12421 acgctttatg gaacacattg cccgacgcaa ggcaggcgat gccgccgaaa ggctggtgaa
    12481 actgataccc gccttctgcc accggatgcc cggctatcct gccgtacagg atgtccgcga
    12541 atccgccgtc gtcaaactcc aagcaggcga tattgtgatg gtcaaacccg gcgaaaccat
    12601 ccccgttgac ggcacggtgc tggaaggaaa cagtgccgtc aacgaatcta tgctgaccgg
    12661 cgagagcctg cccgtcgcca aaatgccgtc tgaaaaagta accgcaggta cactcaacac
    12721 gcaaagcccc ctgattatac gcaccgaccg caccggcggc ggcacgcgac tgtcgcacat
    12781 cgtccgcctg ctcgaccgcg ccttggcgca aaaaccgcgt actgccgagt tggcggaaca
    12841 atacgcctca tctttcatat tcggcgaact cctgcttgcc gtccccgtct tcatcggctg
    12901 gacgctgtac gccgatgcgc acaccgcatt gtggattacc gtcgccctgc tggtcattac
    12961 ctgcccctgc gccctgtcgc ttgccacgcc gaccgcgctg gcagcttcta ccggtacgct
    13021 ggcgcgcgaa ggtattttaa tcggcggaaa gcaggcaatc gaaaccctct cccaaaccac
    13081 cgacatcatt ttcgacaaaa ccggcacgct gacccaaggc aatcccgccg tccgccggat
    13141 tgaactgctc ggcagcatga ccgaagccca agtgctggca gtggcgcaaa gtttggaaca
    13201 gcagtcagaa cacccgctcg cgcgcgccat cctcaactgc cgcatttcag gcggcagcgt
    13261 ccccgaaatc caagtcggac aacgcctcaa ccgcatcggg gaaggcgtgg gtgcgcaact
    13321 gaccgtcaac ggcgaaacac aggtttgggc attggggagg gcatcctatg ttgccgaaat
    13381 ttcaggtaaa gaaccgcaaa cagaaggcgg cggcagcgcg gtttacctcg gcagtcaaag
    13441 cggtttccaa gccgtgttct acctgcaaga cccgctcaaa gacagcgcgg cggaggcagt
    13501 gcggcagttg gcaggcaaaa acctgacgct gcacatcctc agtggcgacc gtgaagaagc
    13561 agttgctgaa accgcccgtg ccttgggtat cgcgcactac cgcgcccaag caatgccgga
    13621 agacaagctg gaatacgtcg aagccttgca aaaagaaggg aaaaaagtgc tgatgatagg
    13681 cgacggcatc aacgacgcgc ccgttttggc gcaggcagac gtatccgccg ccgcagcggg
    13741 cgggacggat attgcgaggg acggcgcgga cattgtgtta ttgaacgaag atttgcgtac
    13801 cgtcgcccac ctgctcgatc aggcgcggcg cacccgccat attatccggc aaaacctgat
    13861 atgggcgggc gcgtacaaca tcatcgccgt accgcttgcc gttttgggct atgtccaacc
    13921 gtggatagcc gcactgggta tgagtttcag ttcgctggcg gttttgggca atgccttgcg
    13981 ccttcacaaa cgggggaaaa tgccgtctga aaaaatactg tccgaacaat gacgggcggc
    14041 gttgctttag acgtatagtc gattaaaaca aaaatgagac gatgaaggat tacaaactta
    14101 aagtatgcat tgttaccgct caaacacgtt ggcgtttaaa atttgagatc gaattgctag
    14161 gtatttgatt ttattaaata agagattata ttaatgagga aaaaaattaa aaagtggatt
    14221 tatgcgataa atggaataat gatgtcaata tggattattt ttttttccaa ataccggttc
    14281 tgtgtatgac ggtggcagaa caaccatttt caatgaaaac catccttttc attttatttt
    14341 ctgcataaca tttcttattg ggataatttt tattatatat caatcatata atgataatta
    14401 atttttaaca tccttactgt cctatcatga tgaaatgaca ataaggatgt tttcctgctt
    14461 tggctacggc aaaacaccgt cgtcattccc acgcaagcgg gaatctatat gtttggtttc
    14521 tcttttattt tcaaacgata atgaaacagg taagtccgga ttcccacctg tgttggaatg
    14581 acggaaatgt gcatttctaa tttttaccca ctataaaaac cccgaatcct gattggcagg
    14641 attcgggttt ttttatcgct gatgccgttc agacggcatt ttcaagcggc ttattgatcc
    14701 acaaacgcgc gctcaatcag gtagtcgccg cgcacgccgg ttttcgggga gactgtcaga
    14761 ccgaaatcgt ccaaaacttt gcaggtgtct ttcagcatcg cagggctgcc gcacagcatg
    14821 gcacggtcgt cttgggggtt gattttgggc aggccgatgt cttcaaacag tttgccgctt
    14881 accatcaggt cggtcaggcg gccgcggtgt tcgaattctt cgcgggaaac aatcgggtag
    14941 taaatcagtt tttctttaac caagtcgccg aggtattcgt gttcgggcaa ttctttggta
    15001 aagcggtcgt agtatgccaa atcctttttg tagcgcacgc cgtgtacaag gatgattttt
    15061 tcaaactgtt cgtaaatttc ggggtctttg gtgatgctca agaaaggggc gatgccggta
    15121 ccggtactca acaggtaaag gtgtttgccc ggattcagat cgcaggcaac tagagttccg
    15181 gtcggttttt tgctgattaa cacttcgtcg ccgactttga ggtgttgcag gcggctggtc
    15241 agcgggccgt cttggacttt aatgctgaaa aattcgaggt gttcttccca gttggcggag
    15301 gcgacgctgt atgcgcgcat cagcggcttg ccgtccgcca tcagcccgac catgacgaac
    15361 tgtccgtttt caaagcgcaa cgattcgtcg cggatgcagg taaaggtaaa atatgcgtct
    15421 gtccagtggt gtacggacaa tactttttgg gtattgaatg ctgccatttg ggtttcctgt
    15481 cagtaaaaga aatggatagt gcttgttcgg gacgtgcggc agagtggaaa tgtctgcccg
    15541 attcgggata aagtcaaaat tctaaacgaa acggatggtt gcgacaatgc ctgatgcgcg
    15601 ttggttatat gccgtctgaa gggcttcgga cggcatcgtg gggcgggcgg tggcttacgg
    15661 cggcatatcg gcgagggaaa ttaaggaaat cgaggctgcc agcctgagcg cgtcccgtcc
    15721 ccagcggggg tgttccagcc tgcgtatcgg gaggatcggt ttgacggtgc cggcaatcag
    15781 cttttgtatt tcggcaggct gatccgacaa tacgccgccc catttttccg cagccgcttc
    15841 cagcagtccg ccgtttttca atatcagcgc ggtcgaatgg atgtagcaga gccaatcgcg
    15901 ggcttggcat tgcgctatgg tcaggacctc ggaagggtcg tcttcaaaat ccaaaaagct
    15961 gatgtttttt ccgtcccaca tcatattccg cgcaaacgcc tgactgagga actgccgttt
    16021 tttatgcacg cgtgcaatgg cttccaaacc ggcaagccaa gcgtccgcct ttccggcttc
    16081 ggcttcttgg cggatttgcg tatcgagcgg gatgccttcc aaattgccga acataagggc
    16141 atttttccgg acggcgagca attcgggaac ggctatccct gccgaacgca attcgtacag
    16201 gcgttttgat tcggttgcaa tggcaggctc gccgccgagg ctgggaaccg gcttcaacac
    16261 ccccagtttc aaatatcggg caaccatacc gagcagcgcg taacgccatc gcgcattgtg
    16321 cctgcctgct ttgcgtaccc ataccttggt cccgtcggca agcaggtagg gggcgatggt
    16381 tgcctcctgt tttgccgcca attcgtccag cagtatagaa aaacgggttt cctgcatagg
    16441 taaggtcatt ttctttcaat cttaagttcg gatggaatgc cgcccatacg gaacatcagg
    16501 caagggtttg ttcgatgatg ccttttaccg catccgcact gttcggcacg gtttgcacgc
    16561 gctgcggcag gttttccaaa ccttccagcg cggcggggcg cggaatggcg acatcgccga
    16621 cggcttcgcg tatggtcgca tcgaatttcg ccgccaacgc ggtttccaaa caaaccaccg
    16681 tttcccccgc ttcgcgcact tcgcgggcga cttttacgcc gttggcagta tgcgggtcga
    16741 tgagttcttt gtcttgctcg taaacctgtc tgatggtggc gaggcggtcg gcgtgggtag
    16801 atttgccgga ggtaaagccg tatttgccgc cgactttgtc caaggcaaac cgcaggtcaa
    16861 agcctttgcc tgccgcgact tccgcccaca gcgtattgat ttcctgagga tcgcgatcca
    16921 tcaggtcgaa cacgaaacgc tcgaagttgg acgctttgga aatgtccata gacgggctgg
    16981 aggttacata agtatgcgcg ctgttgcgcg ggcggtatgc gccggttttg aaaaactcgt
    17041 ccagcacatc gttttcattg gtcgcgacaa tcaggcagcg gacaggcagg cccatttgtt
    17101 tggcgatgtg tcccgcgcaa acgttgccga agttgccgct cggtacgcaa aagctgacgg
    17161 tttcgtcatt gcttgaagtg gcattgaaat agcctgcaaa gtaataaacc acttgcgcga
    17221 cgatgcgccc ccagttgatc gaattgaccg taccgatatg gtatttttcc ttgaacgcgg
    17281 catcgttctg cactgccttc acaatgtcct ggcagtcgtc aaacatcccc ttcacggcga
    17341 tattgtggat attcccgtct tgcaggctgt acatctgcgc gcgttggaac gcgctcattt
    17401 taccgtcggg cgacaacata aatacgttca cgcctttttt gccgcgcaag gcatattccg
    17461 cagccgaacc cgtatcgccg ctggttgcgc ccaagatatt gagttttttg ccttctttgt
    17521 ttaaaacata ttcaaacgca ttgcccaaaa actgcatcgc catatctttg aacgccagcg
    17581 tcgggccgtt ggacaaggct tggattttga tgccgtctga aagcgtgcgg acgggggtga
    17641 tttccttagt accgaacgcc gcttccgtgt aagtacggtt cagaatgtcg cgcaaatcgt
    17701 cctccggaat atcggtaacg aacaggcaca tgatttcaaa cgccaattcg ggataagcca
    17761 aaccgcgcca tttgtccaag gtttcgcgcc cgatttgcgg ataatgttcc ggcagcatca
    17821 gtccgccgtc gggtgcaagc cccatcaata aaacttcgct gaacggtttg tgtgcggttt
    17881 cgccgcgcgt gctgatgtat ctcatgattt ttctcgtttg tcgaaattgc aggaaaacgg
    17941 attcagacgg catctgcctc atgccgtctg aagaaggtta gcggtacagg cgtttgaagc
    18001 aggcggaaac cgttttgacg gtcagggcgg caagtgcctg attgcgcgtg gactggttca
    18061 gcatctgcat cacatcgttg ccggtcagct ggttaggtgc ttcttcggta gcgcagccgc
    18121 aaatcttgtt ttcccattcc gcctgttttt cggcactcat cgccagcgcg gtcaaacgcc
    18181 attcgctgcg cctgtccaat tccgcacggc attggctttc taccgtcatt ttgacgatgc
    18241 tgccacccat acccgtgccg ccgtctaagc tgccgaatgt gttgccgccg gcggcgcacc
    18301 cgccgagtaa gattgccgcc ggcaaaatag acaaggtttt attcatctca attccttttc
    18361 ggttgaaacc ccgcctttta tggcgatagg atctgattag ccgccccgtt cgggataacg
    18421 cgaagggcgg cgttttatgc gccgttccga gtgttggagc aaaccgtttg aatatccggt
    18481 tgaagcccgg caacattata cttcaatcgg gaaaataaaa aatcccgccg ccgtcatttt
    18541 gcctgtttgc aaaaatgccg tctgaaaccg gttcagacgg catttgcgat ttcagcctag
    18601 cccaaagatt tgaactgctc caaaaacgga gggataccgg gcagcatccc gaccgcaccc
    18661 atcgccacac acaagattaa gaaacctact gcgggtatta atactcgtcc gacaaaacct
    18721 aattgcgcgc tgcgttcttt gcagccgatt aagcccaaat tatccaacag catcgtcaac
    18781 gcccagccga aaaccggatt gaccagacgg aagagaacac cacgatggcg gcggattggg
    18841 tggttttgcc tttgcgcgtc atttccatgc ccgcttccaa aagcggtaag tatacgccta
    18901 cgatcaaggc tacgctcaat accggctgcc aaatcgccaa gtccatcgga tagccccaca
    18961 acccggcgat aatacataaa accgccgtta aaaccgcacc gcccggaatg gggcgtttgg
    19021 caatcgatgc cggcacgata taagttcccc aagaagaggt aaaatttgca ccccctaaaa
    19081 tagaacccac tgcttgacgg acagaacaac ttgtcatggt gtcgtctata ttcatcaata
    19141 ccttatcggt tttttccgga tagctgattt tttggaacac ttgatgtcct aaaaaatcgg
    19201 gcgaccacat tgcaacagcc aataccgcaa atggaaagac aaccaaaaaa ctttctgccg
    19261 tcggcaaccc cagatgccag ccgctgtttt caccctacca ataagcagga ctcattggag
    19321 gcaggccggg ggcggtgtga aactcaaacg gcgcacccaa tgcaaatgcc gccacaccgg
    19381 caatcaagca tcccaaaggc acggccaacc agcgtttttt ccaatgctcc aacaaagcgt
    19441 acatcacaat cgttacaata atgacggtaa aagcgatgta gggcatatta aaaccgcctg
    19501 cccacgaaaa caattttttt acctgccccg tcgtgccgat aaagcccaaa tagagtaata
    19561 atccgccgca tacgccgttg cttgtcagct tcgccataat actgccgccg cgaaataaag
    19621 ccatcagcag acctaaaacc gcaatcgaaa tgccgaacgc caaaggatgc ccgcctgccg
    19681 acacaacgat gggaatcatc ggaatcagcg gcccgtgcgt accgggcagg ttggcgccgg
    19741 gcagaaaaaa gcccgatacc aataagataa acgcggcggc gattaaaagc tcatagcgca
    19801 cattttccag tacaaagctg tcaggcagcc ccaaaggtgc ggcaaacgcc gccgcccccc
    19861 caccatcacc acttttccaa tcgttcccgc catcgcagga atcaaatcct cccactcgaa
    19921 gcggtaatcg cgaaagggca ggttgggccg ccagcgtttt ggttgcataa tctgcaattc
    19981 atgttccaaa tattcgtccc gcgtcgcaaa ttccgaagcc ggacggtgta aatcccgata
    20041 agtcccatta tgtttttcca taaccttcct ccttatatat cgcgcctcgt aaaagggggc
    20101 gcatgacttt tctttttgat acgggctgcg ttcggaagcc gtaaccccat ttaaagccca
    20161 aacaggcaat aaaaccaatt tttttttaat aatcatcatc cggaaaactg atacaattta
    20221 caaaccactt gattaaaaag ttaattttca gcaacaatcc gcctaaaaga tttcgattgc
    20281 acaaatatag aaaacatccg cacaaggagg gatacatgga tgccgtacaa ttaaaatcat
    20341 ttgtcgccgt cgcgcacgag ggcaacctta cccaaaccgc caaacgactt ttcctttccc
    20401 agcccgccgt ttccgcccaa attaaagccc ttgaagaata tgtcggcacg ccgctgttca
    20461 ggagcacggg gaaaggcatg gtattgacgc gggcgggcga aatactgttg cccgaagcgg
    20521 aatccctgct gcaatacaaa cacaagttgg agtattttgc caaaacgctg gcaggcgatt
    20581 attcggaaga gaccagtttg ggcattatcc accccatcga ttcggcaaaa ctcgtcgcgc
    20641 tgacggacaa tatcggtcaa acagcccccc aaaacgcgcc tgcacatcca atacggaatg
    20701 agcggcgaaa tcctctcgcg catccaacac aaaaccctgc acggcggctt tatactcggc
    20761 aacgccgccc aaggcggcat ccgcagcgta ttcctgcaaa acctgaccta cgcgctgatt
    20821 tgcccgcaaa gccaatattc ccggctgacc cgctcccttc cgcagagcct gcaagaatgc
    20881 gtatggatag aaatgtcggg cgtgtccgga agtaggaagc acctgcacca gttttggcgc
    20941 agcaaccggc tctcacccaa aaaaacagat attgtgcgac tacccccaaa cccttatcga
    21001 tttggttgca ggcggtatag gtgtggcaat ggtgcaggga aacaaagccg aagcggcggc
    21061 aaaagaaggc gcgggcgtgg ctattatcga atcgtgccgc cacagtatgc cgctcaattt
    21121 catttatgcg gaagaatacg aggataatcc ccacgtctca ctcctgctcg agtgcgttga
    21181 aaaagtatgg ggagtgcagg cggtgcagcc gcccgttgtc tcggacaact gaaataaatc
    21241 ctgctttgct gattgtttta gaatagaaat ttgaatttta tcacgctgaa aacactgaaa
    21301 acgccatccg cattctctcg aatacggctt aaaatgccct ttggaaatgc cgttaaactg
    21361 acgcaaatgc cgttttgcac aattccaaaa gttttcaatt ccgttaatgc aattttgccg
    21421 tttggcgaaa tgcgtgctgt tccgtaaaag ccaaaaaaac cttgcggggc ttgcacgccc
    21481 ccatctgccg cgatccaagt ttgaacaaaa tattggaagg gtttgctgtt gaaacgggaa
    21541 tcgggcaggc aatgtgtggc aataaaatgg ggcctgccga ataatggcgc ggccccgata
    21601 tcattcagtc aaactgatgg gaaacggata agcggatgtt gtacgatcct tttttcccgc
    21661 cggaaaactc ttttttccgc atcggcaaag acattcgttt tttttgcctg tccgcgcatt
    21721 gtagcccaat ccggcggaat accaagtccc cctcgtattt catttttgag cataatgact
    21781 tttgccctca aatgccttaa aggaacgact gcccaaaaat tcatgaaaga tattgccttt
    21841 gatatagaaa agttttttgc ctccgtcaaa ttttctcacg acatccaaac ccaagcggcc
    21901 tatcaggctg tttgcgtggc ttaaattgac gtttatgccg ttatcgatcc gatagccggt
    21961 acctctcaaa taggaatatt gcaactgtac ttgcggtgta atccgccaca atttatcgtc
    22021 atccaaaaat ttacggcggc cgtattcggt gctgatagag aatagggtgt tgctatattt
    22081 tcccttgccc cataattgcc ccatatgatt tcgtgcaatc acacggttac gcagttttcc
    22141 tatttttaat acattgtcga tataccctgt ttcgtctcct tctttatcta tgtattgggt
    22201 agtattgtac agagccaatt catatttcct gattttgtcc ttgccaaacg tattccataa
    22261 tgatgtcctg ccgtggccgt agtcgagact tatgccccat ttgcgttttt tctcctgctc
    22321 gttgaggctg aatcggtcat atccgatttc aaaatcgttg ttatgaaacc taaacgcatt
    22381 ggattgaatc acacgggtat gcgtcagcct tatccaatcg ccttcttttt tatccaacgt
    22441 tgaaaaagcc cgctcccctt cgcgtttggc ataagtatcc aaagtgaacg cataatgtgc
    22501 ggttgaatcg aacatatcat tgatattttt gacatttcgg ctcggattgc cggtagcctg
    22561 tttgaccaaa tagacatttt gcctgttgtc ggtataggtt ttattgacat aatcgtctcc
    22621 agctttttcc gccgtcattt cagagccgtt aaaggattta ttatagtcat ccttgttgtt
    22681 tttatcgccg ttatgtgcgg agtattcgac agtcagggca tcctccataa ggtgcgtatc
    22741 gtcaatatat tttttaccgt tcacaaattc attttttgag tttgttactg tagcgaaacg
    22801 caaacgttcg ccgtaattca tactgtcgag cacttcggac agatttttga cgacgacttc
    22861 ttgcggtgtc gaagtgcctt ttttcacata gagcatatcg ctgtgaatgc cgtctttatt
    22921 cagatgcatc aagaatttat tggcacccgc caattcaccg atatgtaagg cgcgtccact
    22981 tgttttagca tcattattaa aatcgatagt gccttgtcct tccaatttat ccagccagct
    23041 ttgacctgtc atcgtccata atgcgtcttt ggctaaatta aagttaattt cgcccgcact
    23101 tttgctgtct aaatttgcaa catagttttc gaataaattt ttatgtttgg aatcggttaa
    23161 gccgctaaaa ttatccaaac gtcccactaa acggatgttc ggcgtgaaat ctaattccac
    23221 tttaccctta tttaccgcca agacatcgcc tttatagtga atagtgtcgg caacaatatt
    23281 cgtgttattc aaaggtttga tggctaccaa acccttatca gatacggttg cccctgtgat
    23341 acgggtatcg tctttataat tcaaatgaat tgaaccatct tgccttgcaa gattggcaac
    23401 gttggagtca atggtaattt gaccaaatcc acctttgcct ttttgtaggc gaaaacatta
    23461 tatttcgggt ttgaaatcac attatttttt ccccttgagg gaaattgagg agttgttgga
    23521 gtataagccc caaattccgc cttgtacgat attgtcgctc ttcgcagaca attcgacttt
    23581 cgcgttttgc catgaataaa tgccgatatg gcgaaatccc ccatttaatg ccttagattt
    23641 ttcactgatt tcactcttaa cgatattatt ctcgccaatc agtttgacca gcgatttgtc
    23701 gccataggca aaaatgccgt tggaacctcg tttgctttta taatatggtg tctccgttaa
    23761 tactttggaa ttgtttacat aatccgcatc cgcatcgccg gatttcatgg taacaatatt
    23821 atcgccgccg gcttccagta tgattttatt tccatcatcc cttgttccgg gtcttgcccc
    23881 cgttctgtta atcagagtcg aaatgccgtc gctgctgtat gcggcgtttt ccacggtaat
    23941 tttattgttt tctttcgcct tgagggtaat cgtggcattt ccgtgagcgg taatcccttc
    24001 ttggttcatc gggctgcctc ctacttcaac gatattgttt gccgcactca aaactacatc
    24061 gctgtcaatc gtggattgaa ccccccaccc tgtatggttc tggcgaatta ttggggtatt
    24121 tttctttcca ggcattgatt aaagccgtct tggtgtccgg atttttaacc cggacaatat
    24181 tatcctcctc ggcttcaagc gttgcttttg cagaatgcga cgtgataatg ccttttacgg
    24241 aggcgtggtt ttttacattt aaatcaactt cattattccc cttgacggct ttgagttcga
    24301 tggtgctggt gccgtcaagg gaaggttggg tagtattgct tggttgatta ttgatgaaag
    24361 tacccgtcgt gtgtccggaa tagggggagt tggactggag gccatttacg cccaatccca
    24421 agccgtcgcc ctcagtggtg atggatattt tattgttatt ccccgttaat acggtattgg
    24481 atttaaaagc cgtttcaata ccccttaaaa aacttgtact ctcaactttt cctgccggca
    24541 taggctcggc aagcttccct ttaatcgcaa ccacattatc gccgtcttcg gcagtcagat
    24601 taacattggt attgcggagg ttgtgaatac cgaaatggtg tgtgccttta tgaacgttaa
    24661 cgttcatatt gaacaaattg cctgtttttg aatggaagga tatgctgcct ttgtcatttt
    24721 catcggaggg ggtgatattg ttgacatacc gcactaaagc gttatcgttc actaactttt
    24781 cttgatgtgt ggttggatct gttaccttat cggcaacatt caaattaaat tgaatttttc
    24841 ctttggcatc caaatggata tttccggaat agggttgtac gtcaacagat tgaaaatcat
    24901 tgttgaaaat aatgtcatga ttgcttctca ccgataattc gggtttgtat tccaatttta
    24961 ggtctgcaaa agaacggatg ccgtaagaac cgttgaccag taagtctcca tctgcccctt
    25021 ccccggtggt aataagttcc aatttctcat aactgttccc ctttcttcca ataaggcaaa
    25081 atagttttgt ttactgtaat cttaacttcc ccgcatggtt aaaacgggac ctggtctttg
    25141 cgggtccggt tgcaaagctg catggatggc atagcgtata aatttcctga aatttcccaa
    25201 ttgtacgcct aagatcggct tagtacaaac ggtggggcgg tatccgtatg cggcacgccg
    25261 cctttccctg ccgtataatc atattgtctg aaaacctgtt gacggagccg ttatgaccga
    25321 tttgtttgtc cgagaacccg acgcaccgct tgccgaacgt ctgcgcccgc atacgcttga
    25381 tgatgttatc gggcagcagc atttaatcgg cgaaggcaag ccgctgcgcg tggcggtgga
    25441 aggcgggaag ccgcattcta tgttgctgtg ggggccgccg ggcgtgggca agacaacgtt
    25501 ggcgcggatt ttggcgcaga gtttcaatgc ccagtttttg cctgtttccg ccgtgttttc
    25561 cggcgtgaag gacatccgcg gggcaatcga taaggctgaa atcgctttgc agcagggacg
    25621 cgcgacgatt ttgtttgtcg atgaagtcca ccgcttcaac aaggcgcagc aggacgcgtt
    25681 tttgccgcat gtcgaaagcg gtttgctgac ctttatcggc gcaacgacgg aaaatccgtc
    25741 gtttgaagtc aatcccgcgc tgctgagccg cgcgcaggta tatgttttgc aatccttgtc
    25801 ttcagacgac ctcaagaaac tgattgccaa agtgttggct ttgcccgaat accgggattt
    25861 cacgattgaa gccgatgtgc aagagctgct cgtcaatacc gccgacggtg atgcgcgcag
    25921 attattgaat ttgttggaac aacttttacg tgccgccgac acgcgccgtc tgaaaatcct
    25981 gaccaccgaa tttctcgccg acagcttggg cgcgcaaatc cgccgtttcg acaaaggcgg
    26041 cgagagtttc tacaaccaaa tctccgccct gcacaaatcc gtgcgcggtt cgcatccgaa
    26101 cgccgcgctg tattggtttt gccgtatgct cgacggcggc accgacccgc gctatctcgc
    26161 ccgccgcatc gtgcgtatcg cttgggaaga catcgggctt gccgacccgc gcgccttcca
    26221 aatcgccaac gatgccgccg ccacctttga acgcttaggc tcgcccgaag gcgaactcgc
    26281 gctggcgcaa gccgtgctgt accttgccgc cgccgccaaa tccaacgcgg gctacaaggc
    26341 atacaaccaa atgcgccgct tcgtcaaaga aaacgccagc gacgaagtgc ccgtccacct
    26401 gcgcaacgcc ccgaccaagc tgatgaagga attgggctac ggacgcgaat accgctacgc
    26461 ccacgacgaa ccgaacgcct acgccgccgg cgaaagttat atgcccgacg gcttggacga
    26521 accggacttt taccaacccg tcccgcgcgg gctggaaatc aaaatcggcg aaaagctggc
    26581 atggttgaaa tcgctggacg aagaggcatt aaaggcaaaa tgaagcgatg tcgtctgaag
    26641 cagaacagta tggcaaagcc ggtacaccga aaaggcgtat cggctttttt caggtgttat
    26701 aatccgtttc aataaatttt gatgacccat tacgttacgt ttatgtccca aacccacaac
    26761 gaaaattccc gcgtcaaaat ccccgccgtt ttgcacctga tgcgcttggg ctatgattat
    26821 ctttcgctaa aaaatgcaaa ttgggacaga caaaccaata tctttcccga aatctttgta
    26881 gacagcctct gccgcatcaa tcccgatttg ccgccggatg atgcacgccg cctgcttgcc
    26941 gacatacgct tagagctgga taacgaagat ttggggcaaa aattttacga acgcctcacc
    27001 aatcaatccg gcggtaagaa gctgatcgat ttccaaaatt ttgacaacaa cagtttccac
    27061 gttgtaactg agttgccttg tatcaacggc gacgaagcat tccgccccga cattgccctg
    27121 ctggtaaacg gcatgccttt ggtctttatc gaagtcaaaa agcccaacaa taaaggcggc
    27181 atcggcgaag agcgggagcg catgggcaaa cgtgcgaaaa atcccaaatt ccgccgtttt
    27241 atcaacatca cccaattcat gattttttcc aacaacatgg aatacgacga cggcgcaacc
    27301 gagccggcac aaggcgcgtt ttacgcctcg tctgcttgcg gcaagcccgt attcaactac
    27361 ttccgcgaag agcataaatt aaaccttacc gaattattgg acacgctttc agacgacctt
    27421 gaaaacaacg ttctacaaga caataatctg cccgttatca aacacagccc cgaatttatc
    27481 agcaataaat cgcccgatac gccgaccaac cgcatcctga cttcgctgct ttgccgcgaa
    27541 cgtcttgcct ttctcctgca acacgggctg acttacgtca aagcaagtca aggttcggtg
    27601 caaaaacaca tcatgcgtta tccgcaactg tttgccaccc ttgccattga aaaacattta
    27661 tcgaacggcg gtaaaaaagg cgtgatttgg catacccaag gatcggggaa aaccgcgcct
    27721 gcctattaca acacccgtta cctgacccac tattacgcca aacagggtat cgtgccgaaa
    27781 ttctatttca tcgtggacag gctcgattta ttgaaacagg cgcagcggga atttaccgcc
    27841 cgcgacttaa tcgtccatac catcgacagc cgcgaagcct tcgctgccga catcaaatcc
    27901 gcccaaaccc tgcacaacca cgcaggcaaa ccggaaatca ccgtcgtcaa catccaaaaa
    27961 ttccaagacg accccgatgt cgtcgcccgt aacgactacg acctcgccat tcagcgcgtc
    28021 tattttctcg acgaagtaca ccgcagctac aaccccaaag gctcatttct tgccaacctt
    28081 aatcagtccg acgtaaacgc cgtcaaaatc gggctgaccg gcacgccgct tatcggcgta
    28141 accgcaggca acgtcaacac ccgcgaactc ttcggcgact acatccacaa atactattac
    28201 aacgcctcca ttgccgacgg ctacaccctg cgcctgatac gcgaagaaat cggcagccga
    28261 tacaaggcgc aattacaaga agcactggcg caacttgaaa tcgaaaaagg cagctttgac
    28321 cgcaaagaaa tctacgccca tccgcacttc gtccacccga tgcttgacta catcttggac
    28381 gacttcgcca aattcagaaa aaccaaccaa gacgagagcc tcggtgccat ggtcgtttgc
    28441 gacagcgcag aacaggcacg ccagcttttt gagcattttc agacggcctc agaccacaat
    28501 ttcaccgccg cgttgatatt gcacgacgtc ggcactaagg aagaacgcga ccaatgggtt
    28561 aaagatttca aagcgggcaa aaccgacatc ttgtttgtgt acaacatgct tttgaccggc
    28621 tttgacgcgc cgcgcctgaa aaagctctat ttgggcagac tgataaaagc ccataacctt
    28681 ttacaaaccc tgacccgcgt caaccgcacc tacaaatctt accgctacgg ctatgtcgtc
    28741 gattttgccg atatcgaacg cgaatttgac aaaaccaacc gcgcctattg ggacgaactt
    28801 tccaacgaat tgggcgacga aatcggcagt tacagccagc tattcaaaac cgccgaagaa
    28861 atcgaacagg aaattgcaga cattaaaaac gccctgtttg atttcgatac cgaaaacgcc
    28921 gaagaatttt gcagccaaat tagccaaata gaagacaaaa aacagcttct ggccttaaaa
    28981 aaagccctgc aaaccgccaa agagttgtac aacatcctgc gcctgcaagg cagccacgaa
    29041 tttcttgcgc atctggattt tgacaagctc aatcttttgt accgcgaaac cgccgcccgc
    29101 ttggatacct taaaccttgc cgaaaaatta cagcaaggcg acaccgccca tttgctcaat
    29161 gaagcgctgg aagacgttta ctttcaattc gtcaaaatcg gcgaggcgga actcaaactt
    29221 gccgacgatt taaaagacat catgcgcaaa gtgcgcgaag ggctggcggg caacttcgac
    29281 caagacgacc ttgaatacat cagcctgcgc gaagagctgg aacgcatctt caagaaaaag
    29341 aacctcgccg aagtcgggca ggaagaaatg caggcaaaca ttgccacact ccaaaccgtc
    29401 tataccaaaa tcaaagagcc gaaccgacaa aacgacctct tgcggcacaa atacggcggc
    29461 gacgccaaat acgcacgtat ccacaagcgg ttgatggaaa acgccgccct ctacggagac
    29521 aaactcaaag tcttcaacgc gctaaacggc gtcaaaaccg acgccgacca aagagtgctg
    29581 gatatggagc agattttgga caaccagaat tactttgaaa aacaaatgca gggcatcgta
    29641 ttaaaacgct ttagaacgga acagcaattt cctgttcaac ccgcagacat ccagaccatc
    29701 aaccgcctgt tggtacggga atatttaaaa gaaagcgggc ggatttgaga agtagatttt
    29761 caggtagcct taaccattta tagatacagg aaagctatgc acaacaacga catcatcatc
    29821 tacaccacgg aagacggact atccgaattt accctgcgcg agcttgacgg cgaactatgg
    29881 cttgcccaaa aagaaattgc cgaactctac caaaccagca aacaaaacat cggtaaacac
    29941 atcaaagcca tatttgcaga acaagaactg gacgattcag ttgtaaactt tcagtttaca
    30001 actgccgcag acggcaagaa ctaccgatga aatgtttttt tcagacggcc tttgaagagt
    30061 tttgtaacgg aacatcatga atcattttaa gaaacaacaa attcaaaata ttgctgattt
    30121 taatccgaga gagcaactag ccaagggagc attggcaaaa agtgttccga tggcaatgtt
    30181 gaaagaattt caacgacaaa ttacgggtta cgaaatcaag gcatttaacg gtggagctaa
    30241 gtttcgcaat ggtgatacct tattagctaa gattaccccg tgtttggaaa atgggaaaac
    30301 cgcttttgta gatattttag atgatggaga agtggctttc ggttctaccg aatttatcgt
    30361 gttaagagca aaaaatgaaa ccaatcccga atttctctat tattttgcaa tcagtcccga
    30421 ttttagaaaa cgagcgattg aatgtatgga aggaacttcc ggcaggcaac gtgtcaatga
    30481 aaatgcatta aaaacactgg agttgcctat tccagaacct caaatccaac aatccatcgc
    30541 cgccgtccta tccgctttgg acaaaaaaat cgccctgaac aaacaaatca acgcgcgcct
    30601 ggaagagatg gcgaaaaccc tgtacgacta ctggtttgtg cagttcgatt tccccgacgc
    30661 caacggcaag ccctacaaat cctcaggcgg cgacatggtg tttgacgaaa ccttgaaacg
    30721 ggaaattccg aaggggtggg gaagtataga attacaatct tgcttagcaa aaatcccaaa
    30781 tactacaaaa atattaaata aagatattaa ggattttgga aaatatcctg ttgttgacca
    30841 aagtcaagat tttatttgtg gatttaccaa tgatgaaaaa tctatattaa acccacaaga
    30901 tgctcatatt atttttggtg atcatacaag aattgttaag ttagttaatt ttcaatatgc
    30961 gcggggagca gatggtacac aagtaatatt aagcaataat gaacgaatgc caaactattt
    31021 gttttaccaa atcataaatc aaatagattt atcaagttat ggttatgcta gacattttaa
    31081 attcttaaaa gaatttaaaa taattttgcc tagtaaagat atttctcaaa aatataatga
    31141 aatagcaaat actttctttg ttaaagttcg aaataatcta aaacaaaacc accacctgac
    31201 ccaactacgc gatttcctac tacccatgct gatgaacggg caggtatccg taagatgtag
    31261 cggagcaaga gatggataaa ctatttaaaa tattattaaa tataagtagt acatctttgc
    31321 tgttatcaat atattttatt aaatcgcaat attatatatt agaccttact tggttttatc
    31381 gtgtaatttc aaatcattgg atagaaccta tttctaagtt aagtctactt ttttattttc
    31441 taataccatt tatcgcaacc gctactgtct tatggctaag caaatacttg gggaaggatg
    31501 aattcaagca aggagaggtg aaagaattag aatatgttaa cgataatttc ttacctagct
    31561 atcttggtta tttttttgtt gctctaagca tacccgataa taatcttttt ttgctatttg
    31621 ttatgtatgg aattattttc ttactagttt catgctctaa gtcattttat tttaaccctg
    31681 tttttttctt atttggctat cgattttatc aagcaaaaac tgaatcaggg ttattattag
    31741 tgctgattag taaacaggaa tttagaacgc cacaaagtgt aagttcgatt gctgtatatc
    31801 gaattaataa ttttactttc ttagaaaaat aggagttcaa taatgaattt tgttttagct
    31861 aaaattactt gcaagaaaga tttagtaaaa atattatctg atgatcatat tttccctgac
    31921 ttttcttacg agaatttaaa ttttataaca tataactatg attacaatct tgatgatgat
    31981 acttggtttc aaattgaaaa tttaaagaat caagattttt gtcctaagtt tttggataac
    32041 tcaaatttat ttgattcaaa aatgtttagt gaaataaaaa aggaagaaat aaatatagag
    32101 aaactaaagt atttagtatc ttgtactaat gatgcactat tcttccagaa aatcacaagt
    32161 tcattattac tcaaaaagaa gcacttgcta actatatgcg gaaatggtgc gaaactatgc
    32221 gaaccacaag acttattggt tattaaagat attcctgatg ctgtttatat aataaaggat
    32281 gataaattaa tatttagaac cctttcttct atttcaaata tatttaaagg gattgaagat
    32341 ttgtatcgag aagctacaaa tactgaagta cagcaattct tagaaagcga ttttattgat
    32401 ttaaaagaag attttttaag tgaaaaagta tctattccca ataggaaaag aatagcttta
    32461 gttcaagata gattgaataa tatgacgcta gatcaaagac aagaactatt aaattacctt
    32521 gctgaatata ataatatctt gaaatttaac gcagatggct ctcgtgtaga aatttctact
    32581 gatgtacagc tgaaacattt gttatatggt attgatgaac gttattacac aacagcatta
    32641 ggaaaagaga aaaggttggc aaatagtgtt caacctattt aatctgccag caaaccaact
    32701 aatgctaaac aaaatttaag cgacgctagc aacaaaccga gacattccat gaccgaacaa
    32761 cactttaccg aacaaatcaa atccctaatc gacagcttaa aaaccatctg cgccaactat
    32821 ggtttgggca atgacggcaa cgaatttaaa atcatcagtc aggcgttttt gtataaattc
    32881 ctaaacgaca aatacgattt tgaagttaaa aaaattcgca aagaaaagcc cgacgagccg
    32941 atcgagttcg tcaatatgga tattgatgga aaaacagcgg ttttaaagcc cgaacattcc
    33001 atcaagtatt tatcagagcg gcaaaacggc gcggattttg ccaaactctt tgacgatacg
    33061 ctcaccgata ttgccgcctg taatgcggat ttgttttcgg tgaaaaccga aggcggggcg
    33121 aaaatcgtgt tgttcgagcg tatcagtcag tacattaccg acgaaggccg acgcgacgat
    33181 ttttgccgcg cgctgatttc caagctggcg ggtttcagct tcgaggcgat ttttgcgcaa
    33241 aaattcgact ttttcgccac gatttttgaa tacctgatta aagactacaa cagtaattcc
    33301 ggcggcaaat atgccgaata ctacacgccg cacgcggttg cgcggattat ggcggatatt
    33361 ttggtgccgg aggaggtgcg cgggcaaatc cgcagcgtgg acgtttacga cccgtcggcc
    33421 ggttcgggaa cgctgttgat gaatgtcgcc catgtcatcg gcgaagataa atgcatgatt
    33481 tatactcagg atatttcgca gaaatcgtcc aatttgttgc ggctgaatct gagtttgaac
    33541 aatctggtgc attcgctgaa taatgtggtt cagggcaata cgattttgtc gcccgcccac
    33601 aaagacgctt caggctgtct gaaaaaattt gatttcattg tttccaatcc gccgtttaaa
    33661 ttggatttca gcgatttccg cgatcggctg gaaagcgatg aaaaccacga acgctttttt
    33721 gctggtatcc cgaaaatcaa gccaacaaag aaagagaaga tggaaatcta tcagcttttt
    33781 atccagcata ttctgttcag cctgaaagaa aacggcaagg cagcgattgt attaccgacc
    33841 ggctttatca cggcaaaatc gggcatcgat aaaaaaattc gcgaatatct ggtggaaaac
    33901 aaaatgctcg ccggcgtggt gtctatgcct tccaatattt tcgctacgac gggaaccaat
    33961 gtttctatct tgtttattga taaagtcaat aaggataaag ttgtattaat cgatgcttcc
    34021 ggtttaggtg aaaaaattag tattaatgac aaccaaaaaa ctgtactttc ccacgaagaa
    34081 gaacaaaaaa tctgccatac cttcacgcac aaacaggcag tagaagattt cagcgtggtg
    34141 gtcggctatg atgaaatcaa agcgaagaat cacagcttgt cggcggggca gtattttgag
    34201 gtaaaaattg attatgtgga tatatccgcc gacgaatttg tgcaaaaaat agcgggattt
    34261 tcagcggatt tggataaact tttcgccgaa tctgccgaat tggaaaagga gattaaggat
    34321 agattggcga tgttgaaatt caattcataa ataccgatga atgaaaccgg tcaaacttaa
    34381 aatgccgtct gaaagccttt cagacggcat tttgtttggt ttggaaaata cttatgccgt
    34441 tctttccccg aaagagagaa tccaaaaaac caaagccaca ggaattcatc ggaaatgaca
    34501 caaaaacccg acgaaccgga ttcccgcctg cgcgggaatg acgaattaga agttacccga
    34561 aatccgaaaa aagcaaaatc caacccaaca gaccgggttt tcgtttgtac ggaaataatg
    34621 caataaatga agcaaatata aagcatttga atttactata ttatttttcc gcttcttcaa
    34681 agccgacgac ttcgaggccg aagccggtga ggccggtgaa agatgacggt tggccgagga
    34741 cgcgcagttt tttgacgtgg aggccggcga ggatttgcgc gccgatgccg tagcttttgc
    34801 tgtcccattt gtaggcttgg tttgcgcctt tgggtagggt gcggtcgagc agggatgcgc
    34861 cgtcttcggt gtggtgcagg aggatgacga cgccgctttc ggcctgttgg acgcgctcaa
    34921 gggctttggg cagcgaccag gaatggcgcg gattggcttg gatgaagtcc atcacgctga
    34981 agggttcgtg gacgcggacg agggtttcgg tgtcggcgga gggtgtgcct ttgacgaggg
    35041 cgaggtgggt ttcgccggag agtttgtcga cgtaaacgtg ttgttggaac tcgccccacg
    35101 gagtttgtac gggcgcgtcg cccatatctt cgagcaggct ttcggtgcgg ctgcggtatt
    35161 cgatgaggtc ggcaatcgtg ccgattttga gcttgtgttc ttcggcgaat ttcatcagtt
    35221 cgggcatacg cgccatcgtg ccgtcgtcgt tgatgatttc gcagatgacg gcggcgggaa
    35281 tcagcccgtt catttgcgcc aagtcgacgc cggcttcggt gtgtccggca cggacgagta
    35341 cgccgccttt ttgggcgcga agcgggaaga tatgaccggg ttggacgatg tctgcgggtt
    35401 tggcggtcgg ggaaacggcg gtttgaatag tcagggcgcg gtcggcggcg gaaatgccgg
    35461 tggtaatgcc gtgcgcggct tcgatggaaa cggtaaagtt ggtaccgtat tgcgcgccgt
    35521 ttttttgggt catcatcggc agaccgagtt tttcgaccat tgcaccttcc ataggcaggc
    35581 agactaagcc gcgcgcgtgt ttgatcataa agttgacggc ttcgggggta acgaattgcg
    35641 ctgccatcag caggtcgcct tcgttttcgc ggtcttcggc atcggtgatg atgaccattt
    35701 taccggcttt gatgtcggct aggatttcgg ggatggggga aatatgggac atggtgtgtt
    35761 ttcctgtgtg tccgggcggt cggccggtgt gttcggtatc gagccagaag gcgggcattt
    35821 ttgccgcctg ttcgattttg cgggcttttt tctcgccgaa ggatttgttt ttcaaaaggg
    35881 cggagagttc gcctgtgttg agggcaacgg cttcggcaaa acggttttgc agtccgccgt
    35941 agtgttccgc tatccattgc cgcaggttgc ggcggcgcaa gccggctgtg tcggtcatcg
    36001 ttttatcctt tgtagaaatc ggatgtagcg gattgtaagc gcgtatttac taaaaagcaa
    36061 atttgttttt cgccaaatcg gattatttgc cttttggtaa acagatgccg tttgaagccg
    36121 gattcgtggc ttcagacggc attgccgcaa ccccgccggc ggggcggctg tttcttcccg
    36181 cacgcttctg attatggggt ttccggaggg ttattccgaa aaaaattctg ataaaaagcc
    36241 ttgattgccg ggtacggcat aggcggtacg agcaggcgca ataccggcaa tcctatgatg
    36301 aatgccgcct tcccgataaa aaccggacgg atcgcccttt actttctaca agtaacaggg
    36361 cttttttttg cccgtttttg aggatttgca catgg
//
LOCUS       DAXDXW010000013        35875 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXDXW010000013 DAXDXW010000000
VERSION     DAXDXW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35875
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.013"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            254..517
                     /locus_tag="ACFB64_000536"
     CDS             254..517
                     /locus_tag="ACFB64_000536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208954.1"
                     /translation="MSSAQQANYPQNLFPTKNNPQVCTKGNPPYRHPFKTAIQMILKI
                     KKSYPQKIPHINIIIVLLFKPYILFITLQGNGTAKRKGIFSKN"
     gene            597..2045
                     /gene="gnd"
                     /locus_tag="ACFB64_000537"
     CDS             597..2045
                     /gene="gnd"
                     /locus_tag="ACFB64_000537"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC1208955.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKETGIIGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFVEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAGKGILFVGAGVSGGEEGARRGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLAEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGRLFARTATPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLGNIRDAYEANPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2108..3379
                     /gene="waaA"
                     /locus_tag="ACFB64_000538"
     CDS             2108..3379
                     /gene="waaA"
                     /locus_tag="ACFB64_000538"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC1208956.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPHP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRRRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEDMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLAVVPRHPEHFQTTFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVGSGCQNII
                     EPLSCGVTTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKNLSGEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3420..3869
                     /locus_tag="ACFB64_000539"
     CDS             3420..3869
                     /locus_tag="ACFB64_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC1208957.1"
                     /translation="MSIYAVAHIIHLYCATAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFAVGLLFASGIVMAANRYLPISGEPFATSFGTMLTLKILLAFSV
                     LAHFAVAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            3902..4876
                     /locus_tag="ACFB64_000540"
     CDS             3902..4876
                     /locus_tag="ACFB64_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC1208958.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGIKET
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAAAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEEDLANSRLLNAVKKVVPVGTAFHGEKFFTVENGKKIATPLFLVLIMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            4945..5020
                     /locus_tag="ACFB64_000541"
     tRNA            4945..5020
                     /locus_tag="ACFB64_000541"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4978..4980,aa:Lys,seq:ttt)
     gene            5241..6494
                     /gene="murA"
                     /locus_tag="ACFB64_000542"
     CDS             5241..6494
                     /gene="murA"
                     /locus_tag="ACFB64_000542"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC1208959.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVATTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVNQHLKGLEAMGAEIAIEHGYVKAKGKLKGARVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGA
                     GELYGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVERIY
                     HLDRGYENIEKKLGSVGANIERVSG"
     gene            6575..7768
                     /locus_tag="ACFB64_000543"
     CDS             6575..7768
                     /locus_tag="ACFB64_000543"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC1208960.1"
                     /translation="MAFLKLTEQNVRGKTVLIRADMNVPFKGGKISDDTRIRASLASV
                     KYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYAALCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTEQIGYISTGGGAFLEFLEGKELPAVAALEKTRR
                     VNGLI"
     gene            complement(7820..8068)
                     /locus_tag="ACFB64_000544"
     CDS             complement(7820..8068)
                     /locus_tag="ACFB64_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC1208961.1"
                     /translation="MLTPEQVKALIAGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8161..9078)
                     /gene="ftsX"
                     /locus_tag="ACFB64_000545"
     CDS             complement(8161..9078)
                     /gene="ftsX"
                     /locus_tag="ACFB64_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC1208962.1"
                     /translation="MSIIHYFSLHVESARSALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLTRDKRLDNIRFIGKEDG
                     LAELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSIFSAAVSLGLCGWLLSAVHPLVDAIFKPYGLNIGWRFFYVGELG
                     LVFGFVIALGVFGAWLATTQHLLCFKAKK"
     gene            complement(9075..9725)
                     /locus_tag="ACFB64_000546"
     CDS             complement(9075..9725)
                     /locus_tag="ACFB64_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC1208963.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSMGKVLFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILNDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(9919..10401)
                     /locus_tag="ACFB64_000547"
     CDS             complement(9919..10401)
                     /locus_tag="ACFB64_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC1208964.1"
                     /translation="MKRLNLAAIALAATFAAHTASADELAGWKDNTPQNLQSLKAPVR
                     IANLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            10525..10878
                     /gene="arsC"
                     /locus_tag="ACFB64_000548"
     CDS             10525..10878
                     /gene="arsC"
                     /locus_tag="ACFB64_000548"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC1208965.1"
                     /translation="MSEIKIFHNPRCSKSRAAVSLLEERGIAAEAVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            10875..11531
                     /locus_tag="ACFB64_000549"
     CDS             10875..11531
                     /locus_tag="ACFB64_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC1208966.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            11647..13041
                     /gene="gltX"
                     /locus_tag="ACFB64_000550"
     CDS             11647..13041
                     /gene="gltX"
                     /locus_tag="ACFB64_000550"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC1208967.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCAVVDDYDMGVTHIIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTTEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPDGKLTELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAKAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALISKEEVLKRIRA"
     gene            13062..13457
                     /locus_tag="ACFB64_000551"
     CDS             13062..13457
                     /locus_tag="ACFB64_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208968.1"
                     /translation="MPSDVGMRLQTAFRRKLNMKKILYFLMFVFSTSVWAGGAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEEMRQIGVRLDHAWNAVYGAGERKRRGN"
     gene            13461..13952
                     /locus_tag="ACFB64_000552"
     CDS             13461..13952
                     /locus_tag="ACFB64_000552"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC1208969.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDSGTLLGSAAVKRHDMENFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(14228..16822)
                     /gene="mutS"
                     /locus_tag="ACFB64_000553"
     CDS             complement(14228..16822)
                     /gene="mutS"
                     /locus_tag="ACFB64_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC1208970.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYELFLDDAVEAA
                     KLLDITLTTRGQMDGVPIKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTADK
                     LNDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDSKEHAVSIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKTPTLFSILDGCATHMGSRLLALWLHHPLRNRAHIRARQEAVTALESQYEPL
                     QCHLKSIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSATGSSLLETLKAVFP
                     ETLPVAETLKAAVMPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQDQALALEKQLFDGVLKNLRTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVVHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPVDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSI
                     VLMDEVGRGTSTFDGLALAHAIAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGRDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKAAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVDCFVDKAEEKHFEGILAAALENLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17253..18257
                     /gene="gap"
                     /locus_tag="ACFB64_000554"
     CDS             17253..18257
                     /gene="gap"
                     /locus_tag="ACFB64_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC1208971.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVAAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGREIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPATKEEINAAMKAASSESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18409..19089)
                     /gene="tsaA"
                     /locus_tag="ACFB64_000555"
     CDS             complement(18409..19089)
                     /gene="tsaA"
                     /locus_tag="ACFB64_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC1208972.1"
                     /translation="MTYTITPIGAARSPYKQKFGIARQPGLVSAAEACIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGSDLLDGTPIVDIKPYIPFIESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     INLSPTPL"
     gene            19549..20184
                     /locus_tag="ACFB64_000556"
     CDS             19549..20184
                     /locus_tag="ACFB64_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFC1208973.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMAAKWLVGNPVDMLKPTGLSDADLTWVAHAMVGTRGYELLDGLPSVKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTNMVIVGGLATDYCVKTTVLQL
                     LKGGRWQVIVNEAACRGIAPDTIEAAWQEMRSSGAIILKNAEKIKKYINNQ"
     gene            20221..21189
                     /gene="waaC"
                     /locus_tag="ACFB64_000557"
     CDS             20221..21189
                     /gene="waaC"
                     /locus_tag="ACFB64_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC1208974.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCSDVELHWLCEAGFAGI
                     ARLHPFVKKVHVMKWRQWRKHLFQAETWREMGRLKQALLQEAFDFVLDSQGLIKSACF
                     AKMAKSPIYGLDKHSAREGVAALAYDKKYVVPKGRNAVWRNRDLFAQVFGYAMPETQV
                     FGLSVPEASRLKNLAQPYYAALHATSRDSKLWPMENWRELLQKLNQKQQCNVYLPWGN
                     EAEKVRAEQIADGLPFTIVCAKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSAIAKNVGNIGQIPTADLVYQTLMDCVSADKG"
     gene            21415..22164
                     /locus_tag="ACFB64_000558"
     CDS             21415..22164
                     /locus_tag="ACFB64_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFC1208975.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHIETDVKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFVSKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLDSADLATDISPRLKTV
                     KFAEPKARQTGVKVASVAELVEKLKNEAKVI"
     gene            22175..23110
                     /locus_tag="ACFB64_000559"
     CDS             22175..23110
                     /locus_tag="ACFB64_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC1208976.1"
                     /translation="MSVLIIAEHDNKQLNPATLHAVAAAAKLGKVDLLVVGNGASSVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRVTAFDAVAAQGGN
                     AEVINVKTTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSVEKFSEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLIELLKN"
     gene            23173..23784
                     /locus_tag="ACFB64_000560"
     CDS             23173..23784
                     /locus_tag="ACFB64_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC1208977.1"
                     /translation="MITTYKTITSPTQAEFKDKGSRFIAFAYPIRTLADVKKYLNLLK
                     EEHYKARHWCYAYRLGVNGMQFRGNDDGEPPGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGALLGVPGLIHAYKEATAQALAIAEVVEKNIEKTVWLKCEYPFLNEAICIAKQYQAD
                     ILEQDLQLDCRLTVSLSLVNYEACVAAWKNTRQVEVNTEKPFE"
     gene            complement(23836..24174)
                     /locus_tag="ACFB64_000561"
     CDS             complement(23836..24174)
                     /locus_tag="ACFB64_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC1208978.1"
                     /translation="MKKEQANTQKRIDEIQSKYREWCHLLLQLEENIRRWKYAVALIR
                     DMDNFYTHEYQVRHQTIEDGVELNLQTEGEYSIMSEDALWNAPGEFHQLAWLYLCSSV
                     DTLDRYTQEN"
     gene            24311..25582
                     /gene="purD"
                     /locus_tag="ACFB64_000562"
     CDS             24311..25582
                     /gene="purD"
                     /locus_tag="ACFB64_000562"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC1208979.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIESKLQ
                     NIALTAYQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMVKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLVAGKGVIVAMTLD
                     EAHAAIDDMLLGNKMGNAGERVVIEDFLQGEEASFIVMVDGNHVLPMATSRDHKRLLD
                     GDKGPNTGGMGAYSPAPVVTPAVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLADLVEAAIDGRLDSVKAEWNPQTAVGVVL
                     AAQNYPETPKKGDVISGLDDVNRIGKVFHAGTTVNEKGDVLTNGGRILCVVGLGDDVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25616..26218
                     /locus_tag="ACFB64_000563"
     CDS             25616..26218
                     /locus_tag="ACFB64_000563"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC1208980.1"
                     /translation="MTDALQTAFYIMLFSRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQLEQLQPLLQMPSENIQTMLRNEWPA
                     PKTFLLLAKSGVLPALRGKRRSKLAVRVPDHTGARRLCQALGMPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            26233..26748
                     /locus_tag="ACFB64_000564"
     CDS             26233..26748
                     /locus_tag="ACFB64_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208981.1"
                     /translation="MKTFLHTQRKNFLDGISLHDCYRTAIKVEGRNVCFDFEDGFVVL
                     DNNPNNQAGKHLKTDFSRVVLTHENRNAEDDYLVDIFEDIVFLGKRLFTVRKFLDFSE
                     LLEMINAQGYFLEFLYLYECIGVKTSDYFLEAVLVTGRSRECKKCFIKIMRASSFVYQ
                     WNNLRLNSEIV"
     gene            complement(26900..27340)
                     /locus_tag="ACFB64_000565"
     CDS             complement(26900..27340)
                     /locus_tag="ACFB64_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC1208982.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(27572..27751)
                     /locus_tag="ACFB64_000566"
     CDS             complement(27572..27751)
                     /locus_tag="ACFB64_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC1208983.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYMHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            complement(27757..28344)
                     /locus_tag="ACFB64_000567"
     CDS             complement(27757..28344)
                     /locus_tag="ACFB64_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC1208984.1"
                     /translation="MPLPDLTDAELMESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSNEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            complement(28366..29112)
                     /locus_tag="ACFB64_000568"
     CDS             complement(28366..29112)
                     /locus_tag="ACFB64_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC1208985.1"
                     /translation="MQPETSAQYQHRFSQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSKEWSRLKEGFVRDARAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTHDLQEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPKADNWKNILLGKWSG
                     WIEQRIIIPSLSAISENMEGNSPSQNHLSA"
     gene            complement(29102..29944)
                     /locus_tag="ACFB64_000569"
     CDS             complement(29102..29944)
                     /locus_tag="ACFB64_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC1208986.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGRAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMCRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLENVDAGRVCYIHIAGHDAETPELLIDT
                     HGAAVLPTVWDLLELAYTKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            complement(30062..30370)
                     /locus_tag="ACFB64_000570"
     CDS             complement(30062..30370)
                     /locus_tag="ACFB64_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1208987.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAAHKPASNATGVQKSAQGSCGA
                     SKSAEGSCGAAASKAGEGKCGEGKCGATVKKAHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            complement(30444..30893)
                     /locus_tag="ACFB64_000571"
     CDS             complement(30444..30893)
                     /locus_tag="ACFB64_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC1208988.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNFAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPKCRLKQD"
     gene            31234..32127
                     /locus_tag="ACFB64_000572"
     CDS             31234..32127
                     /locus_tag="ACFB64_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC1208989.1"
                     /translation="MVTFSKIRSFLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVA
                     LALGGGASKGFAHIGIVKVLKENGIPVKVVTGTSAGSIVGSLLASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHKYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PSKNVGQGFFSYLDQTLNVMSVSVLQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            32230..33333
                     /gene="prfB"
                     /locus_tag="ACFB64_000573"
     CDS             32230..33333
                     /gene="prfB"
                     /locus_tag="ACFB64_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC1208990.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKESKILEGIVLTLDNIASGIEDNRMLIEMAVEENDEEGFAAVK
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            33412..33771
                     /gene="rnpB"
                     /locus_tag="ACFB64_000574"
     ncRNA           33412..33771
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFB64_000574"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(34100..35557)
                     /locus_tag="ACFB64_000575"
     CDS             complement(34100..35557)
                     /locus_tag="ACFB64_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC1208991.1"
                     /translation="MSRHPARTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGITLIAALATAIKTGLVNLDNFSRILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT         8688 a         8232 c        10098 g         8857 t
ORIGIN      
        1 gttaatccgc tatgcttata cacaaccagc caaaagccat cttattgaaa tatcataaga
       61 tttactcaaa aatcttattt tctccaccac ttatgcacaa actcggatac acactctgcc
      121 aatatgcaga aaaaaaagac attcgcaccg acaccttttc atccccacaa gttccaaaca
      181 catttccggc ttaaatatcc acagcattcc cgttcttaaa ggcaccggca gccattcggt
      241 aaagaaaagc gggatgtcgt ccgcacaaca agcaaactat ccacaaaacc tattccccac
      301 gaaaaacaat ccacaggtat gcacaaaagg gaatcctcct taccgacacc cttttaaaac
      361 ggcaattcag atgattttaa aaataaaaaa aagttaccca cagaaaattc cccatatcaa
      421 tataatcatc gttttattat ttaaacctta tattttattt ataacccttc agggaaacgg
      481 cacggcaaag cgtaaaggca tcttttcaaa aaattaaaaa gttaaggatt gttaaacaag
      541 tgtaaatcag gtagagttgc agccgataaa ttaaatctat aacggagctt cctgcaatga
      601 aaggcgatat tggcgtaatc ggtttggcgg ttatggggca aaacctgatt ttgaatatga
      661 acgattgcgg atttaaggtt gtcgcctaca accggacaat cggtaaagtg gacgaatttt
      721 taaacggtgc ggcaaaagag acgggcatta tcggcgcgta ttccctgcaa gacttggtcg
      781 ataagctggc aaaaccgaga aaaatcatga tgatggttcg cgcaggttcg gtggttgacg
      841 actttgtcga acaactgctt ccgctgttgg aagaaggcga catcattatc gacggcggca
      901 atgccaatta ccccgacacg acacggcgga cacattacct tgccggaaaa gggattttgt
      961 ttgtcggcgc gggcgtatcc ggcggcgaag aaggcgcgcg gcgcgggccg tccatcatgc
     1021 cgggcgggga taagcgcgct tgggaagcgg tcaagccgat tttccaggcg attgccgcca
     1081 aaacgccgca gggcgaaccg tgttgcgact gggtcggcaa ggatggcgcg ggacattttg
     1141 tcaaaatggt gcacaacggc atcgaatacg gcgatatgca gctgatttgc gaagcgtacc
     1201 agtttatgaa agacggtttg gggctgtcct acgacgaaat gcaccgcgtg tttgccgagt
     1261 ggaacaaaac cgagctggat tcttatctga ttgaaatcac agcggcaatt ttgggctata
     1321 aggacgaagg cggcgaaccg ctggccgaga aaatcctcga tacggcaggg caaaaaggca
     1381 cgggcaaatg gacgggcatc aatgccttgg atttgggcat tccgctgacg ctgatttccg
     1441 aagccgtatt cgcccgctgt gtttcgtcgt tcaaagaaca gcgcgtgcag accggcaggc
     1501 tgttcgcccg aaccgccacg cccgtcgaag gcggcaaaca agaatgggtc gaggcgttga
     1561 gacaggcttt gcttgcctcc aaaatcattt cctacgcaca aggctttatg ttgatccgcg
     1621 aagcgggcga aagttacggc tgggatttgg actacggcaa cactgcgctg ctgtggcgcg
     1681 aaggctgcat catccgcagc gcgtttttgg gcaatatccg cgacgcgtat gaggccaatc
     1741 ccgatttggt gttcttgggt gcggatccgt atttcaaaaa tattttggaa aactgcctgc
     1801 cggcatggcg caaggtggtt gccaaggcgg tcgaatgcgg catccctatg ccctgcatgg
     1861 cttcggcgat tacgttcttg gacggctata cgaccgaacg cctgcctgcc aacctcttgc
     1921 aggcacagcg cgactacttc ggcgcgcaca cttacgagcg caccgacaaa ccgcgcggcg
     1981 aatttttcca taccaactgg acgggcaagg gcggagatac cgcttcgaca acctacgata
     2041 tttgattccg ttgccgaaat gccgtccgaa ccgttcggac ggcatttgtt ttgcaggaaa
     2101 tagaaagatg ttccaatggc tttatgatgt attgtggctg cttgcgccga tatggatacg
     2161 gcgttatttg gacaaacgct ccggaagtgc cccggcatac cgggcgcata gggacgagcg
     2221 tttcggcaag ccgcatccga atcccgttac cggcgcggtt tggatacacg ccgtttcggt
     2281 cggagaaacg cgtgccgccc agcccttgat acgcgagttg cggcggcgtt ttcccgatgc
     2341 gccgctgctg atgacgcaga tgaccccgac gggacgggaa accgcgcaag ttctgtttcc
     2401 cgatgcgcaa tgccgctatc ttccgtatga caaaaaaacg tgggtacggc agtttttgcg
     2461 cgaacaccgc ccgatgttcg gcattttgat ggaaaccgaa atctggccca atctgatgaa
     2521 ggaatgccgg cgagcgggtg tgccgctgtt tttggcgaat gcgcggctgt cggaaaaatc
     2581 gctgaacggt tatctgaaag tccgccgcct gatccgtccc gcagccgctt cgctgacggg
     2641 ctgtctggcg cagacggagg cggatgcggc gcggttggcg aaattgggcg cggcatccgt
     2701 gcaggtgtgc ggcaatacca aatacgacct gatgccgtct gaagacatga aaacgctggc
     2761 ggggcagttt gaaaaacgca tcggaggccg gccggttgcc gtgtgcggca gcacgcgcgt
     2821 ttatcggggc gaagacgagg cggaaaaact gctggcggcg tggcaacaat atcgcggcga
     2881 tgccctgctg gccgtcgtgc cgcgccatcc cgagcatttt cagacgacat ttgaaacggc
     2941 gaaacgcttc gggtttaagg ttcagcggcg cagcgacggt ttgccggtcg aacccgatac
     3001 gcaggtgtgg ataggcgaca gtatgggcga gctgtatgcg tattacctgt gcgccgatgt
     3061 cgcttttgtc ggcggcagtc tggtcggttc gggctgtcag aacatcatcg aaccgctttc
     3121 ctgcggcgtt acgacgatat tcggcttttc gacctacaat ttttccgaag cctgccggca
     3181 cgccttggca tcgggtgcgg cggttcaagt cgaatcggcg gacgcgtggc gtgaagccgt
     3241 tgaaaaaaac ttatctggcg aggggggcgg aatgcagatg caggcgcgcg tggacggctt
     3301 tatcgcacaa catcgcggag cgggcgcgag aatcgccgag gcggtgcggg aagcggtatg
     3361 cggacatcgg gggcgatagt gatacaatcc gtatccgagt tttccggttg gagtaccgta
     3421 tgagtattta tgccgtcgcg cacatcatcc acctgtattg cgccaccgcc tttgtcggcg
     3481 gcgtgttttt tgaagtgctg gttttgtccg tcctgcatac gggacgggtg tcgcgcgagg
     3541 cgcggcgcga agtggaaaag gcaatgtctt accgcgccgt cagggtgatg ccgtttgcgg
     3601 tcggactgct gttcgccagc ggcatcgtga tggcggcaaa ccgctacctt cctatatcgg
     3661 gcgaaccgtt tgccacttcc ttcggtacga tgctgacgct gaaaatcctg ttggcgttca
     3721 gcgtgttggc gcacttcgcc gtcgccgtcg tcaaaatggc gcgttccacg ctgacggtcg
     3781 gctggtcgaa atacatacac gccgtcgtct ttacccatat gctgctgatt gtctttttgg
     3841 caaaagcgat gttttatatc agctggtaac acaactacat ttcaaatcag gagattgaac
     3901 catgacagaa tatcccggca tcgggtcgcc gttgttttac ggcgtttttt ttgcggcagt
     3961 gctggtcatg attgccttgg atatgttttc gctgaagaaa aacggcagcc ataaagtcgg
     4021 catcaaagaa accttggcat ggagcggctt gtgggttgcc gtatcctgcc tgttcgcagg
     4081 ctggctgtat ttcgaactcg ccggcaatcc cggctacggc gcggcagccg caaaagaaaa
     4141 agtattggaa ttctttaccg gctacatttt ggaaaaatcg ttggcagtcg acaatatttt
     4201 cgtgttcctg atgatattcg gctacttcaa agtcgcaccg cagtttcaac accgcgtgct
     4261 gctgtacggc gtattgggcg cattggtatt gcgcaccgtc atgattttcg tcggcgcggc
     4321 actggttcgg cagttcgagt ggattctgta tctgttcggc gcgttcctgc tctataccgg
     4381 catacacatg atgaagcccg aaggagatga ggaagaggat ttggcaaaca gcaggctgct
     4441 gaatgccgtc aagaaagtcg ttccggtcgg cacggcgttt cacggcgaga aattttttac
     4501 cgtcgagaat ggcaaaaaaa tcgccacgcc gctgtttttg gtgctgatta tgattgaatt
     4561 gagcgatgtc gtttttgccg tggacagtat ccccgccgtc tttgccgtta ccaccgatcc
     4621 gtttatcgtg ctgacttcca atattttcgc tattttgggt ttgcgggcga tgtatttcct
     4681 gctggcggat gtggcggaac gctttatctt cctgaaatac ggcttggcat tcgtgttggg
     4741 ttttatcggt gtgaaaatgc tggtgatgca ttgggtacac atcccgattt ccgtttcgct
     4801 gtcggtcgtg ttcggcgcgt tgggtgcatc gatactgacc tcgttaattt atacgaaaaa
     4861 acagcctgat aaataaaacg ccgtctgaac gccgacaaaa tggttgacag catcaggctt
     4921 gatgtataaa ctttcaaatt tttcgggtcg ttagctcagt cggtagagca gcggactttt
     4981 aatccgttgg tcgagcgttc gaatcgctca cgacccacca ggtaaagcaa aaaagccgag
     5041 ttttcaaaac tcggcttttt tttgttttcc gacaatcctt gttcggaaag cgcggcattt
     5101 cgtcaaattc tttggtgcgg cttccgtatt tttcccgaaa atcccttata attccgcctc
     5161 tgcaaccttc atcgggtgat gctttatgca ttgtcccgag tattgaaata ttagcaagta
     5221 cccaccagga gggaacaaac gtggacaaac tgaaaatctc cgcaaacggc ccgctcaacg
     5281 gggaaataac ggtctcgggc gcgaaaaacg cggcattgcc gctgatgtgc gcgggtttgc
     5341 tgacgtcggg tacgttgcgc ctgaaaaacg tccctatgct ggcagatgtc gcaaccacgc
     5401 aaaagctgct tcagggcatg ggcgcgcgcg ttctgaccga caatatcagc gaatttgaaa
     5461 tcaacggcgg tacggtcaac aatacctgcg ccccttacga attggttcgg acgatgcgcg
     5521 cttcgatttt ggtgcttggc ccgacgctgg cgcgtttcgg cgaggcgcaa gtcagcctgc
     5581 cgggcggctg cgccatcggt tcgcgccccg tcaatcagca tttgaaaggc ttggaagcga
     5641 tgggggcgga aatcgccatc gaacacggtt acgtcaaagc caaaggcaaa ctcaaaggtg
     5701 cgcgcgtggc gatggatgtc gttaccgtcg gcggtacgga aaacctgctg atggcggcga
     5761 cgctggcgga aggtacgacg gttttggaaa actgcgccat cgagcctgaa gtggtcgatt
     5821 tggcggaatg cctggtcaaa atgggcgcga aaatcagcgg catcggaaca tcgaccatga
     5881 ttgtggaagg tgcgggcgag ctgtacggct gcgaacacag cgtcgtcccc gaccggattg
     5941 aagcggggac gttcctgtgt gcggtggcga taaccggcgg cagggtggtt ttgcggaatg
     6001 ccgccccgaa aacgatggaa gtggtgttgg acaaactggt cgaggcaggt gcggtgattg
     6061 aagcgggcga cgattggatc gccatcgata tgcggcagcg tccgaaggcg gtggacatcc
     6121 gcacggtcgt ccatcccggc ttccccacgg atatgcaggc gcagtttatg gcattgaatg
     6181 ctgtggcgga aggaagctgc cgcgtggtgg aaacgatttt tgaaaaccgc tttatgcacg
     6241 ttccggagtt gaaccggatg ggggcgaaca ttacaaccga gggcaatacg gcatttgtgc
     6301 agggtgtgga acggctttcc ggcgcggtcg tcaaggcgac ggatttgcgc gcgtccgcca
     6361 gcctcgttat cgccggtttg gcggcgcgcg gcgaaaccgt ggtcgaacgg atttaccact
     6421 tggatcgcgg ttatgaaaat attgaaaaaa aactcggcag cgtcggtgcg aacatcgagc
     6481 gcgtatccgg ctgatcgggt tgcgatgccg tctgaaggct tcaggcggca tcaattgttt
     6541 ctgtatcgca aacactgatt gaaaaggact gattatggca tttttaaaac tgaccgaaca
     6601 aaacgtgcgg ggcaaaaccg tcctcatccg cgccgatatg aacgtgccgt tcaaaggcgg
     6661 caaaatcagc gacgacaccc gtatccgcgc ctcgctcgcg tccgtcaaat actgcttgga
     6721 caacggcgcg tccgttattg tgatgaccca cttgggccgc ccgaccgaag gcgagttcca
     6781 ccccgaagac gacgttgcgc ccgttgccgc gcacttgggc ggcctgttgg gcaaagacgt
     6841 gaaagtattg aacgactggc gcgaaaacaa accggctttg aacgcgggcg atgtcgtgat
     6901 gctgcaaaac gtgcgtatca acaaaggcga gaagaaaaac gatttagaac tgggcaaagc
     6961 ctacgccgcc ttgtgcgacg tgttcgtcaa tgatgcgttc ggcaccgcac accgcgccca
     7021 agcttcgacc gaagccgttg cccaagccgc gcccgtcgcc tgcgccggcg tattgatggc
     7081 gggcgaactc gacgctttgg gcaaagcctt gaaacagccc gcgcgcccga tggtggcgat
     7141 tgttgccggc agcaaagtgt ccaccaagct gaccatcctc gaatcgttgg cggacaaagt
     7201 cgatcagctt atcgtcggcg gcggcatcgc caacacattc ctgttggcgg aaggcaaagc
     7261 catcggtaaa tcgctggcgg aacatgattt ggtggaagaa tccaaaaaaa ttatggcgaa
     7321 aatggcggcc aaaggcggtt ctgtgccgct gccgaccgat gtcgtcgttg ccaaagcctt
     7381 tgccgccgat gccgaagcgg tggtaaaaga catcgccgac gttgccgaag acgagatgat
     7441 tttggacatc ggcccgaaat ccgccgccgc gcttgccgag ttgcttaaag ccgccggtac
     7501 ggtcgtttgg aacgggccgg tcggcgtgtt cgagttcgat caattcgcag gcggcacaaa
     7561 agcccttgcc gaagccatcg cccaaagcaa agcgttctcg attgcgggcg gcggcgacac
     7621 gctggcggcg attgccaaat tcggcgttac cgaacaaatc ggctacatct ccaccggcgg
     7681 cggcgcgttc cttgagttct tggaaggcaa agagctgcct gccgtagccg ctttggaaaa
     7741 aacgcggcga gtaaacggtt tgatataaaa aaatgccgtc tgaaacgaaa tggtttcaga
     7801 cggcattttt gtgtggcggt tattgtgctt tggcagccca ttccgccgga gtggcggcaa
     7861 ccgaaatgga aagtgcgtgc agttcgttgc tttccagttg ggctttgagt ccgtctttaa
     7921 tcaggcggtg gcgcgcgagg cgtgccttgc cttcaaattc tgatgaaacg atgacggcga
     7981 aaaaatggtg tccgtcgcct tctacttcga tgtgttcgca ttttgccacg cctgcaatca
     8041 gggccttgac ttgttcgggt gtcagcatag tggggagtcc ttattgggga aatgcgccat
     8101 tatactggca aatcgaaatt ccgtctgaaa acggcttcag acggaatttt tgacggtgtt
     8161 ttattttttg gctttgaagc agagcaggtg ttgtgtggtg gcaagccacg cgccgaatac
     8221 gcccaacgcg atgacgaagc cgaacaccag cccgagttcg ccgacgtaga agaaacgcca
     8281 gccgatgttc aagccgtagg gtttgaagat ggcatcgacc aaggggtgca cggcagagag
     8341 cagccaaccg caaagcccta agctgacggc ggcggagaag atactctgcc acatagcctg
     8401 ataaaggaag gggcggcgga taaacgacgc gggcgcaccc aggagtttgg tgatttcgat
     8461 ttcttctttg cggctgagga tttgcagtcg gatggtgttg tgtgcgacaa ggacgaaagc
     8521 catacccagc gtcagggaaa gaaaccataa gattttgcgg atgaactcgt tgatttggta
     8581 cagcgtttgc acccattcgg tatccataga cgcggattcg accataggca gcttggtaat
     8641 gtcccggtag attgcctgca tttgggcggg cgtggttgcc gggtcggggg taacgataaa
     8701 gacatccggc agggggttgc cgtcaagcat ggaaatcaga ttttggtcga ggttggactg
     8761 taattccgcc aaaccgtctt ccttgccgat gaagcggatg ttgtcgagcc gtttatcgcg
     8821 cgtcagcagg ctgcgtacgg tatcgctgtc gctttgtgcg gcggcggttt ccatatagac
     8881 ggtgatttgc ggcgactcgt tgagtttgcc caacacgctt tgcccgcttt ggatgcccag
     8941 atacataaac agcggcaggg tcatcgcgac ggcaagcatt atgagggtaa gcagtgtgcc
     9001 gaagggttgg cgcagaagct gtttgagtgc ggagcgcgcg gattcgacgt gcagcgagaa
     9061 gtagtggatg atgctcatgc gagtcgtcct ttcgagaggc gcaggatgcg gtgtccgtag
     9121 tccgccatca gggtttcgtc atgtgcggcg acgatgacgg tggttcccgc ttcgtggaag
     9181 gttttgaaca attccataat atcgagcgcg taggcacggt cgaggttggc ggagggttcg
     9241 tcggcaatca gcaggccggg ctggtggacg acggcgcggg cgatgcacag gcgttgttgt
     9301 tcaccgcccg agagggttac gggatcgtcc aattctcgtc ctttcaagcc gactttttcg
     9361 atggcgatgc gggcgcgttc ttcggctttg cgcggcggat agccgataat ccgcaggggc
     9421 aggatgacgt tttgcaggac attgcggtcg ttgaggattt tgtggtcttg gaacacgatg
     9481 ccgatgtgtt ggcgcataaa gccgatttgg ttgtcggaca atgtgccgag gtcctgcccg
     9541 ttaaacagga ctttgcccat gctcggcttg gtgatgcccg aaatcagctt gaggacggtg
     9601 gatttgcccg aaccggagtg tcccgcgata aaaatcatct cgcctttgtt gatttggaag
     9661 ctgacgtttt tcagggcttc aaaaccgccg ggataggttt tggaaacttg ttcgaaacgg
     9721 atcataggag gtcctgtaag gaagggcggc atatcgtttc gggcggttgc gtccgcaggg
     9781 atatgccgtc tgaacaatag gtaagacagt accgattata aagattccgc ctgtttttag
     9841 atagtggata agtttggcgg ataccgaagg ccgggcggga aaatgccgtc tgaagcggct
     9901 tcagacggca tccggcgttt aacggcattt tgaatgggcg agtttgacgg cttcggtcag
     9961 gcttttctcg ttcacctctc ctgtaatggt ctgcctgtat ccgcatttcg gggcttcgac
    10021 gacggtaaag ggcagtacgc cgacattgtt tccgtaggat ttcataaagt ttcggctgtt
    10081 cgccccggtg taacgccaaa tcgggtagct gaccggggtc tgcttgagaa agttgccgat
    10141 attgtcggat gtgtcgagcg cgatgccgac catatcgacg ctgccttttt tctgcgcttt
    10201 gtaccatttg gacatggcgg gcatctcttt tcgacacgga ccgcaccaag tcgcccatag
    10261 gttggcgatg cggacggggg ctttgagcga ttgcaggttt tgcggggtat tgtctttcca
    10321 tccggccagt tcgtctgccg aggcggtatg tgcggcaaat gtggcggcaa gggcgattgc
    10381 ggcgagattc aagcgtttca tcgttttgtc ctttgtatgt cgattcgggt attgtaaacc
    10441 gtttggtacg gttggaggcg gggtattgag taaaaccctg cgtaagccga taataagggg
    10501 attgtcgaga agtagggaaa tgtcatgtct gaaatcaaaa ttttccacaa tccgcgttgc
    10561 agcaaatcgc gtgccgccgt gtccctattg gaagaacgcg gcattgctgc cgaagcggtc
    10621 aaatatttgg atacgccgcc cgacttgtcc gaattgaagg atattttcaa caaattgggc
    10681 ttggaatcgg cgcgcgggat gatgcgcgtg aaagatgatt tgtacaagga attgggtttg
    10741 gacaaccccg atttggacaa tgacgcgctg ctgcgtgcca tcgccgatca tcccgccctg
    10801 ttggagcgtc cgattgtttt ggcaaacggt aaggcggctg tcggcagacc gttggaaaat
    10861 atcgaagcgg tattgtgaac aaacggctgt tttgcagccg caacggcttg cgctattacc
    10921 tgttgggcgg tttctgtttg agtgtttttc cgcttttgct ggtgtttgcc tcatccgttt
    10981 gggcggttta tcggacgggc gggcaggttt tgccgccgta tgtccgggcg gatgccgcgc
    11041 tggtgttggg cgcagccgcg tgggacaaac gcccttcccc ggtttttcgg gaacgcatca
    11101 accacgccat cgcgctttac caaagccgca gggtaggcaa aatcgtgttt acgggagggc
    11161 gaaccaaaaa aggctatatg accgaagccg aggtcgggcg gcggtacgcg ctcaaacagg
    11221 gggttccggc gcgcaacatc ctgtttgaaa acacttcgcg caacacttat gaaaacctga
    11281 acaacatccg tccggttttg cgtgccaacg gtattgccag cgtggtgatt gtcagcgatc
    11341 cctaccattt ggcgcgggcg gcggaaatgg cggaggattt gggtgtccgc gtttatatgt
    11401 ctgccacgcc gaccacgcgg tttgatgcgg gaaataagaa aaaaatcttt atgctgcaag
    11461 aggggtatgc gttatccctc taccgtttgg aaaaatgggg cagccgcttt ttgggatggc
    11521 tgtctgattg aagcggtaga aaatgccgtc tgaaacttat ccgcaggcag ggttcggacg
    11581 gcatttttga tgcctaagca cggtaaaaac agtaaaataa acgctttcaa agatttcgga
    11641 gccattatga ccgtcaaaac ccgtttcgcc cccagcccca ccggttacct gcacatcggc
    11701 ggcgttcgca ccgccttgtt ttcgtgggcg tttgcccgcc atcataaagg cgaattttta
    11761 ttgcgtatcg aagacaccga cttggcacgt tccaccgccg aatccgtcaa catcatcctt
    11821 gacggcatga aatgggtcgg tctcgattac gacaacgccg acaacgtggt gtaccaaacc
    11881 cgccgtttcg accgttataa agaagtcatt gccgaactct tggcaaaagg cgatgcctac
    11941 tactgctatt gcagcaaaga agaattggaa gcgatgcgcg agaaagccga aaaagaaggc
    12001 acggcgactt acgaccgccg ctggcggccg gaagcaggca aaaccctgcc cgaaattcct
    12061 gcaggcgtgc aacccgtcgt ccgcttcaaa acgcctttgg acggcgttac caaatggaca
    12121 gacttggtca aaggcgaaat ctccatcccc aacgaagccc tagacgacct gattattgcc
    12181 cgcgccgacg gtacgccgac ctacaacttc tgcgccgtgg tagacgacta cgacatgggc
    12241 gttacccaca ttatccgcgg ggacgaccat gtgaacaaca ccccgaaaca aatcaacatc
    12301 ttgaaagcca tcggcgcgaa cctgcccgaa tacgggcatc tgccgatgat tctcaacgaa
    12361 caaggcaaaa aaatctccaa acgcagcggc gataccgtcg ccattaccga tttcggcgca
    12421 atgggcattc tgcccgaagc gatgctcaac tatttggcgc gtttgggctg ggcgcacggc
    12481 gacgatgagt ttttcacgac ggaacaattc atcgaatggt ttgatttgaa agacgtttcc
    12541 ccgtccccaa gccgtatgga tttgaaaaag ctctattgga tcaacggcga acacatcaaa
    12601 atcacacccg acggcaaact taccgaactc gtcaaaccgc gccttgcgct gcgcgatatt
    12661 catgaaaccg aaaaacctgc gttggaagac gtgttggcac tggtcaaaga ccgcgcccaa
    12721 gatttgaacg ccttggcaga cgaatgcctg tatttctaca aaaaacaagt ccctgccgaa
    12781 gccgatgttg ccaaacactg ggacgacgaa gccgccgccc gtatgctgcg cttcgccgaa
    12841 cgcctcgaag ggctggaaga ctggaacgcc aaagccattc acgacctctt caaacctttc
    12901 tgcgacgaag aaggcatcaa aatgggcaaa ctcggtatgc ccctgcgcct tgctgtctgc
    12961 ggtacggcaa aaaccccgag cgtcgatgcc gtgttggcat taatcagcaa agaggaagtg
    13021 ttgaaacgca tccgtgctta agttatccac aggtgcgggc aatgccgtct gatgtcggaa
    13081 tgcggcttca gacggcattc agacggaagt tgaacatgaa aaaaatattg tattttctga
    13141 tgtttgtttt ttctacaagc gtatgggcag ggggtgcgga agacaatttg ctcagcatcc
    13201 aatccggtta ccgtgcttta ctgcaaaagc aaaacaatct ggacggaaaa atcatcggga
    13261 tgcagtcgga tttggaagat gcgcgccggc gtttgcaggc ggctcaggca gacatcgccc
    13321 gtttggaagc ggaaattcct gcagcaatgg cccaaaaagc ccggcaggct gaagagatga
    13381 ggcaaatcgg agtgcgtttg gaccatgctt ggaatgcggt ttacggcgca ggggaacgaa
    13441 agcgtcgggg gaattgaccg atgaattccc tctttgtgga caacactgtt tttattacac
    13501 ggctgaaagc cgggcatatc ggcaggttgg ttcaggcgtt gtttgaggag tggcacggat
    13561 ttgaaccgtg gtcttctgtg gataagattc gtgcctatta cggcagatgt ttgaaggatg
    13621 acgaattgcc gctggctttt gcggctgtgg atgattccgg aaccctgttg ggttcggctg
    13681 cagtcaagcg gcatgatatg gaaaattttc cacagtatga atattggttg ggcgatgtct
    13741 ttgttttacc tgaatatcgc ggaaaaggca ttggcaggag gctggtcgcc cactgcatag
    13801 gcgcagcgcg ttcgctgggg ataaagttct tgtatcttta tacgcctgat gtgcaaatat
    13861 tttatgaatc attcggctgg gcggttgtcg ggcgacattt ccataacggg gaatgggtta
    13921 cggttatgcg tttggatgtg gataaggttt aagccgaatg ggcagatgcc gtctgagctg
    13981 ttcggacggc attttttatg tttggaaatt tcctgtcaga tatagtggat taacaaaaac
    14041 cggtacggcg ttgccccgcc ccggctcaaa gggaacgatt ccctaaggtg cccaagcacc
    14101 gggcggatcg gttccgtacc atttgtactg cctgcggccc gccgccttgt cctgattttt
    14161 gttaatccgc cataaagttt atccacagat ttttccatat ggtttgatgc tgctccgcca
    14221 acggaaatta agatacggat ttgcacaaat ctttcagacg gtacagttct gacaatgctt
    14281 cgcgcggggt caggctgtcg ggatcgaggt tttccagggc tgctgccaat ataccttcaa
    14341 aatgtttttc ctctgcttta tccacaaagc agtccacatt cggttcatct cctttttcag
    14401 acggcatggt actgaaaata tccagttggg gacggtttgc ggcggcttgg ttttccagtc
    14461 cgttcaaatg cttttgggcg gctttcaatg cgcgcacagg caggccggcg agtttggcga
    14521 cggcgatgcc gtagcttttt ccggcgggac cgggttggat ttggtgcagg aacacgatgt
    14581 cccgtccctg ttcgagcgcg gaaaggtgca tattgacggc ggcggcgtgg gcttcgggca
    14641 ggtaggtcag ctcgaaatag tgggtggcaa acaggctgaa ggatttgttt ttttgcagca
    14701 ggtgttcggc gattgcgtgc gcgagggcga ggccgtcgaa agtggaagta ccacgtccga
    14761 cttcgtccat taaaacaatg ctttgttcgg tggcgtgatg caggatgtag gcggtttcgc
    14821 tcatttcgac catgaaggtg gagcggttgg aggcgaggtc gtccgatgcg ccgatgcggg
    14881 tgaagatttg atcgacaggc ccgattgtgg cggcatcggc aggcacgaaa cagccggtgt
    14941 gtgccaataa aacaatcagc gcgacttggc gcatataggt ggatttgccg cccatattgg
    15001 ggccggtgag cagcatgagg cggtgtttgt ggtcgaggtc ggtgtggttg gctgtgaagt
    15061 ggcgtacctg ctgttcgaca acgggatggc ggccgttttc gatgtggaca accggatagt
    15121 cggcaaactc gggacggaca aagttccgct cttttgccaa ggctgaaaat gtggacaaca
    15181 cgtccagcgc ggcggcggct ttggcggctt tttgaagctg cggcaatgcc gtccgaaggt
    15241 ttttcaatac gccgtcaaag agttgttttt ctaaggcgag ggcttggtct tgagcagtca
    15301 gcactttgtc ttcaaaggct ttcagttccg gcgtgatgaa gcgttcggcg tttttgaggg
    15361 tttgccggcg ttggtagtcg gcaggtgctt gttcggcttg ggttttggac aattcaatgt
    15421 aaaagccgtg aacgcggttg aactcgactt tgagtgtgga caaaccggta cgttcgcgtt
    15481 ccttggcttc caaatccagc aaaaattcgt cgccatggtt ttgaatgcgg cgcaattcgt
    15541 ccagttcggg atgaaaaccg tggttgatga cattgccgtc tttcagccag acggaaggtt
    15601 cgggcatcac ggcggctttg agggtttcgg caactggcag ggtttcgggg aaaacggctt
    15661 taagggtttc caacaggctg ctgccggttg cggacaagtc gatttgtgcc aactcgaaca
    15721 gactgtcgcg taaagatgcc aaatcgcgcg ggcgggcatt gccgacggcg atgcgggcgg
    15781 cgatacgttc gatgtcggca atgctcttca gatggcattg gaggggttcg tattggcttt
    15841 ccagcgcggt tacggcttct tggcgcgctc ggatgtgggc gcggttgcgt aaagggtggt
    15901 gcagccacaa tgccaacagg cggctgccca tgtgggtggc gcagccgtcg agtatggaaa
    15961 acagggtcgg cgtttttttg ccggagaggg tttgcgtgat ttcgagattg cggcgcgtgg
    16021 cggcatccat gccgatatat tggctgtcgg tttcgagcga caggccgtcc aaatgttgcg
    16081 gcatcaggtt ttgcgtcagg cggatatagt tcaacagcgc acctgccgcg ccaatcgaaa
    16141 cggcgtgttc tttgctgtcc aaaccgaagc cgcgcaaatc ttggcagccg aaatattccg
    16201 tcagcagttt ttcccccgcg tcggcggcaa actgccacgc gttcaggcgt gtaacacccg
    16261 atgccgtctg aagttgcggt gcgtttttac tgtccggcag caaaatttcc gccgcctgca
    16321 ggcgcgccag ttcgtcgttc aatttatccg cagttgtcag cttggttttg aattcgccgc
    16381 tttgcagcga tgcccacgcc aaaccgatgt attttttgtc gggagacacg gcaacgatgc
    16441 ggttggtttc cttgtcttcc agtaatgcgg aatcggttag cgtgccgggc gttacgatgc
    16501 gcacgacttt gcgctccacc ggccctttgc ccgcgccgac ttcgccgacc tgttcgcaaa
    16561 tcgccacgct tttgcccaac ttgaccaggc gcgccagata ctgttccgcc gcgtgaaacg
    16621 gaacgcccgc cattttaatc ggcacgccgt ccatctgccc gcgtgtggtc agggtgatgt
    16681 ccaacaattt cgccgcttcc accgcgtcat ccaaaaacag ctcgtaaaaa tcacccatac
    16741 ggtaaaacac tagtttgtcg gtatgttgcg cttttatgcc gaggtattgc tgcatcattg
    16801 gggaaacggc ggatttggac atggggctgc cttattatta ggaaaacggt attgtagcaa
    16861 aaggttttgt cttcgacgga atgagactga ccgtaagctt gtcaataaag ttgtggagac
    16921 aaataggaaa acggcaaagt tatccataag aatattatta agtcaaaaaa catccacgct
    16981 tatctgtctt atacggcaag ccttaaactc aaatttattt aacaaaaagg taatgaaaat
    17041 attggatatt tgctacttat acacaagcag gtcggcaatc atcaaaatca tcattaaaat
    17101 tttaaaatca aatattgaat gcgcgttttt gaaatagcga aattcacctg cgtcaaacaa
    17161 aacgcaagta attttatgta atagtggaat gtagttttat ttcagaatac aatacaggca
    17221 tcggacttta tttcacaacc gggagacaaa atatgagcat caaagtagcg attaacggtt
    17281 tcggccgcat cggccgcctc gcattgcgtc aaattgaaaa agcccacggc atcgaagtcg
    17341 ccgccgtcaa cgacctgacc cctgccgaaa tgctgctgca ccttttcaaa tacgacagca
    17401 cacaaggccg tttccaaggt actgccgaat taaaagacga tgccatcgtg gtaaacggca
    17461 gagaaatcaa agttttcgcc aatccgaatc ccgaagaatt gccttggggc gagttgggtg
    17521 tggatgtcgt cctcgaatgt accggtttct ttaccaacaa aaccaaagcc gaagcacata
    17581 tccgtgccgg cgcgcgcaaa gtcgtgattt ccgcacccgg cggcaatgat gtgaaaaccg
    17641 tcgtatatgg cgtaaaccaa gacatcttgg acggtagcga aactgtcatt tccgccgctt
    17701 cctgtaccac caactgcctt gccccgatgg cggcagtcct gcaaaaagag ttcggcgtag
    17761 tcgaaggcct gatgaccacc atccacgcct acaccggcga ccaaaacacc cttgacgcgc
    17821 cgcaccgcaa aggcgatttg cgccgcgccc gcgccgccgc gctcaacatc gtgcccaaca
    17881 gcaccggcgc cgccaaagcc atcggtctgg ttatccccga attgaacggc aaactcgacg
    17941 gctccgccca acgcgtccct gttgccaccg gctcgctgac cgaattggtt tccgtcctcg
    18001 aacgccctgc aaccaaagaa gaaatcaacg ccgcgatgaa agccgcctcc agcgaatctt
    18061 acggctacaa cgaagatcaa atcgtttctt ccgacgttgt cggcatcgaa tacggctcgc
    18121 tctttgacgc aacccaaacc cgcgtgatga ccgtgggcgg caaacaactg gtgaaaaccg
    18181 ttgcctggta cgacaacgaa atgtcctaca cctgccaact cgtccgtact ttggaatact
    18241 tcgcaggcaa aatctaaggc ttgaacaaac ctgtggataa gtaaatgccg tctgaacaca
    18301 ttgcgttcag acggcattgt ttatctgtaa cttgatattg cggcttatgg cgaacaaaca
    18361 acagccgttt gttcaactgc tatgcggcaa aaccggattt tgcccgattt aaagcggggt
    18421 tggggaaaga ttaataaccg tcgcgcggtt ttcctcgatt tgaaacctga cttcgtaatc
    18481 cgcaatattc atcacataaa tccgttcggg aatattctga taggcggggc gcggatcttg
    18541 ggcaatgctt tggctgataa ggtttttggt gtttgcagat aaattttccg cgccgatgtt
    18601 ttcctgccaa acgacttcca actctacggg tttgccgctg acgaaaccgg atgcggcatc
    18661 gggtttggat tcgataaagg ggatataagg cttgatgtcc acaatcggcg tgccgtccag
    18721 caggtctgag ccgctgcaat agaggcggac gggtttgccg gtttcgatgc gttcgagttt
    18781 aaggagagag agtccgagat ggttggggcg gtgggggctg cgcgtggcga acacgcccat
    18841 tttttgtttg ccgccgagcc gtggcgggcg caccatttgc gcccaacctt catccaatac
    18901 gccgtggaag ataaaactta tccacacata atcgaaatct tccaacccgc gcacgctgtc
    18961 tgcggtaaat tcgggattca gctcgatgca ggcttctgcg gcggagacca aaccgggctg
    19021 gcgggcgatg ccgaatttct gtttgtaggg cgagcgggcg gcgccgatgg gggtaatggt
    19081 ataagtcata agattgtgga taagtttttt ggaaaaacct tatcttatca cagccggttt
    19141 taccgctttc gggtagtaat ttgttataat acagggcatt taaatcgcgt tgaaataaag
    19201 tagcacagaa tcctcgtgtc caagacgctc ttaaaaaggc attggagtat tgataaaaaa
    19261 gaagcataac ggttcgttat aacgaagagg gaatcccgta tattacaccc aagtcaggtt
    19321 gttatctttc cttttggatc tcacttacag gaggtgagat ctgaaatttt gggtaataaa
    19381 atcggattac agcttctagc aaggagtacc ttaggtatag ctaaaatgga gggggtggat
    19441 atgacatatc atatccacct agatggattg tactccttaa ataaagaagg taagagtttt
    19501 gttattcgta aaatagagaa tgacgatctt aaatgaagaa gtaatgagat gattgtttcc
    19561 attgatgttg atgcacaaaa aacttttacg cctttgtgtc ctgacgaact gcccgtgaac
    19621 gaggggcatt tgattgtcga ggagttaaat gcccaagccg ctttggcgga tttgcgtgtg
    19681 atgacgaaag atgcgcatca tatggcggcg aaatggcttg tgggtaaccc tgttgatatg
    19741 ttgaagccga caggtttgtc cgatgcggat ttgacttggg tggctcatgc gatggtcggt
    19801 acgcgcggct atgaattgtt ggacggtctg ccttccgtca aggaatacga ttattgcgtt
    19861 tggaaaggtg tcgatcccga attgcatcct tacggcgcgt gttttcacga tattgaggaa
    19921 aaactgagca ctggattgat tgagtggctg cgttgtcaaa atacgaatat ggtcattgtc
    19981 ggcggcttgg ctacggatta ttgtgttaaa acaacggttt tgcagttact taaaggcggt
    20041 cgttggcagg ttatcgtgaa tgaagcggct tgtcggggta ttgcgcccga taccattgaa
    20101 gcagcgtggc aggaaatgcg ttcttcgggt gcgataattt tgaaaaacgc cgaaaaaatt
    20161 aaaaaatata ttaataatca ataacttata attgtttcac gtgaaacacc ctgtctacat
    20221 atgaaaattt tgcttgtccg tttgtccagt atgggtgatt taattcacac tttgcccgca
    20281 atcgaagatt tggcgcgaca atgctctgat gtagaactgc attggctatg tgaagccggg
    20341 tttgcgggta ttgcacggtt gcatccgttt gtaaaaaagg tccatgtaat gaagtggcgg
    20401 caatggcgca aacatctctt tcaggctgaa acttggcggg agatgggtcg tctgaaacag
    20461 gctttgctgc aggaagcatt tgattttgta ttggacagtc agggtctgat taaaagcgcg
    20521 tgtttcgcca aaatggcaaa atcccctatt tatggtttgg ataaacacag tgcgcgtgag
    20581 ggggtggctg ctttggcgta tgataaaaag tatgtcgtac cgaaaggtag aaatgccgtt
    20641 tggcgcaacc gtgatctgtt tgcccaggtg tttgggtatg caatgcctga gacgcaggta
    20701 tttggtttgt ccgttcctga agcaagccgt ctgaaaaatt tagcgcagcc gtattatgcg
    20761 gctttgcacg cgaccagtcg ggacagtaag ttatggccta tggaaaattg gcgggagttg
    20821 ctgcagaaac tgaatcaaaa acaacaatgc aatgtttatc tgccttgggg aaacgaggct
    20881 gaaaaagtgc gtgcggaaca gattgcagac gggctgccgt ttaccatagt gtgcgctaaa
    20941 atgaatttat tacaggcggc gtatctgttg aaacacgcgg ttggaatagt tggtgtagat
    21001 accggtttgc tgcatttggc aaatgccttg gaaaaacctg tggtcggcat ttataccgat
    21061 accgatccga ttaaaacagg cgttcaagtt tcggctattg caaaaaatgt gggcaatatc
    21121 gggcagattc cgaccgcaga tttggtttat caaacgctga tggattgtgt ttcagcagat
    21181 aaaggctaaa gggtcgtcta aaactgatat ggcggcatga tattttccaa tttattagat
    21241 acaggaatgt atatccgcca gactgagtaa atactttaaa aatggtataa tcggtaaaat
    21301 tatccgaaag attgctaaat aaattcgttt ttaaggaaaa cggatttgat tttataaaga
    21361 agaaggtttg gtttcgctgg atggtgaaag cagcgttctt tttaggagaa ttcaatgaaa
    21421 gcactggtcg cagtaaagcg cgtagtggac tacaacgtca aagtccgtgt gaaagccgat
    21481 ggttcggatg tggacatcgg caatgtcaaa atgtcgatga acccgtttga tgaaatcgct
    21541 gtggaagaag ccgtccgttt gaaagaagcc gggaaagtaa gcgaaattgt agcggtttct
    21601 ttgggtgaaa agaaatgcga agaaaccttg cgtaccgctt tggcaatggg tgccgaccgt
    21661 gccattcata ttgaaaccga tgtgaaatta gagccgctgg cagttgccaa actgctgaaa
    21721 gctgttgcgg acaaagaaaa tccgcaaatt ttctttttgg gcaaacaagc gattgatgac
    21781 gatgccaatc aagtggcgca aatgctggca gctttgttga atgcggcgca aggtacattt
    21841 gtttcaaaag tacaaattga aggtgatgaa gtacagattg tgcgcgaaat tgatggcggc
    21901 gaagaaacga ttgcgttgaa actgcctgca gtcatcagcg cagatttgcg tttgaacgag
    21961 ccgcgctttg tcaaactccc caatattatg gcggcaaaga aaaaacctct ggaaaaactg
    22021 gattctgccg atttggctac cgatatttcg cctcgtctga aaacggtgaa atttgccgaa
    22081 cctaaggcgc gtcagacagg tgtaaaagta gcaagcgttg ccgaattggt tgaaaaattg
    22141 aaaaacgaag ccaaagtgat ttgaggaggc tgaaatgagc gtattgatta ttgccgaaca
    22201 cgacaacaaa cagttgaatc ctgccacttt gcatgctgtt gccgctgccg ccaaactggg
    22261 caaggtcgat ttattggttg tcggaaacgg cgcatcgtcc gtcgtggaat tcgcgaagca
    22321 agtagcggga gtggaaaaag ttttggttgc agatgctgct cattatgctg aaggtttggc
    22381 tgaagagttg gctccgctgg ttgtcaaatt ggcggcggat taccgctatg ttgcagcaac
    22441 ggcaaccaca ttcggtaaaa accttttgcc ccgcgtagcc gccttattag acgtaccgca
    22501 aatttctgat ttgaccgaga ttgtagataa cacgactttt gtacgtccga tttatgcagg
    22561 taatgcattt gaaaccgtgc aagccgattc agaaaaattg gtgctgacct tccgtgtgac
    22621 ggcttttgac gcagtggcgg cgcaaggtgg taatgctgag gtaattaatg ttaaaacaac
    22681 ccctgcccaa aacctgagtc gttttgttaa ccgtcaactt tcccattccg atcgtcccga
    22741 actgactcag gcaaaagtga ttgtttccgg tggtcgtgcg ttgggtagtg tggaaaaatt
    22801 cagtgaagtg ctgacaccgt tggcggatgt tttaggtgcg gcaatcggtg catcccgtgc
    22861 ggcggttgat gccgagtatg caccaaacga tgctcaagtc ggacaaaccg gtaaggtggt
    22921 tgcgccgcag ctctattttg caatcggtat ttcaggtgcg attcaacacg ttgccggtat
    22981 gcaggacagt aaggtgattg ttgcaatcaa caaagatgcc gatgcgccga ttttcaatgt
    23041 agccgattac ggattggttg gcgatttatt tgaaattgtt ccacaattaa tagaattatt
    23101 gaaaaattga tgtttcacgt gaaactttca ggcgactttg ttattaatga ggtcgtctga
    23161 aaacatttca gcatgattac gacttataaa accattactt ccccgacgca ggctgagttt
    23221 aaagataaag gcagccgttt tattgcattt gcctatccga ttcggacatt ggctgatgtg
    23281 aaaaaatacc tcaatctgtt aaaggaagag cattataaag cgcggcattg gtgctatgcc
    23341 tatcgtttag gtgtgaacgg gatgcagttt cgcggcaacg atgatggaga gccaccagga
    23401 agtgctgggc ggccaatttt gggacagatt gattcggtgg gtatcactga tgttttggtc
    23461 gtggtcgtcc gctatttcgg tggtgcttta ttgggcgttc ccggcttgat tcacgcatac
    23521 aaagaggcaa cggctcaggc gttggcgatc gcggaagtgg ttgaaaagaa tattgaaaaa
    23581 accgtttggc tgaaatgtga gtatccgttt ttgaatgaag caatatgcat tgccaaacaa
    23641 tatcaggcgg atatattgga gcaggatttg cagttggatt gccgattgac ggtaagtttg
    23701 tcgttggtaa attatgaggc ctgtgttgcg gcttggaaga atacacggca ggttgaagta
    23761 aatacggaaa agccttttga atgaaaggtt gtatgaaatg ctttgtaatg gtatttcagg
    23821 caacctcttc gctgattaat tttcttgtgt atatctgtct aaggtatcga cgctggagca
    23881 taaatatagc caagccaatt gatggaattc gcccggcgcg ttccataatg catcttcact
    23941 cataatgcta tattcgcctt ctgtttgcaa attcagttct accccgtctt caatagtctg
    24001 atggcgcacc tgatactcgt gggtatagaa attgtccata tcgcgaatta aagcgacggc
    24061 atatttccaa cggcggatgt tttcttccag ttgcagtagt aaatgacacc attcgcggta
    24121 tttgctttgg atttcatcaa tacgtttttg cgtgtttgct tgttcttttt taatttgtga
    24181 ataagttgaa taggttgcag agtggaatga agtggtcttt tgtttgattc tactaaaatt
    24241 tcttcagatg ggacaaaagt tcctttgtat cgagttttcg ttaaaagtac atatttgtca
    24301 aggagatggc atgaaactgc tggttatcgg taatggcggt cgcgaacacg cgctggcttg
    24361 gaagttggcg cagtcgccca aagtggaaac agtatttgtt gcgcccggta atgccggtac
    24421 ggcgattgaa tccaagctcc aaaacattgc cttgaccgcg tatcaggatt tgattgaatt
    24481 ctgccgtaaa gaaaatattg tttttaccgt cgtcggccct gaagcgcctt tggcggcagg
    24541 tattgtggat gattttcgtg ccgcagggct gaaaatattt ggtccgacac aatatgcggc
    24601 gcagttggaa agttctaaag atttcgccaa agcatttatg gtgaaataca atattccgac
    24661 cgcgcaatac caaacctttg aaaatgccga tgccgcacat gattacgtca atcagaaagg
    24721 tgcgcctatc gttatcaaag ccgatggttt ggtggcaggt aaaggcgtga ttgtggcaat
    24781 gactttggat gaagcgcatg ctgcgattga cgatatgttg ttgggcaata aaatgggcaa
    24841 tgccggcgaa cgcgttgtga ttgaagattt tctgcaaggc gaagaagcga gctttatcgt
    24901 tatggttgat ggcaatcatg tattacctat ggcgaccagt cgagaccaca aacgtctttt
    24961 agacggagat aaaggcccga atacgggcgg tatgggtgcg tacagtcccg cgcctgtggt
    25021 aacgcctgct gtgtacgagc gtgcgatgaa cgagattatt ttgccgaccg tagcgggtat
    25081 gaaagcggag gggcacgagt tcaccggctt tttgtacgca ggtttgatga tcgatcaaag
    25141 cggtgcaccc tatacgattg agtttaactg ccgtttcggc gatcccgaaa cccagccgat
    25201 tatgagccgt ctaaacagcg atttggcgga tttggttgaa gcggcaatag acggcagact
    25261 ggacagcgtc aaagcggaat ggaatccgca aactgccgtg ggtgtggtac tggcggcgca
    25321 aaattacccc gaaacaccca agaaaggcga tgttatttcc ggtttggatg acgtcaaccg
    25381 aatcggcaaa gtattccatg ccggcacaac ggtaaacgag aaaggcgacg tgttgaccaa
    25441 cggcgggcgc atattgtgtg ttgtaggatt gggcgacgat gtggcgcaag cgaaagccaa
    25501 ggcgtatggc gcgttggaaa aaatcagctt cgacgggatg caataccgta aagacattgc
    25561 cgataaagcg attaaccgtt aattagaaag aggatgatgc cgtctgaagc caagtatgac
    25621 tgatgcgctt cagacggcat tttatattat gttattttcg agaattatcg cagcatcagc
    25681 gcaacgtaag ttaagcgttt atttgaaaaa aggcggtttg gttgcttatc cgaccgaatc
    25741 ttgttatggc ttggggtgtc ttccgacatt agccaaagca ctcggtaagt tggcgcattt
    25801 gaaaaaaagg ccgcaacaca agggtatgat tgtcatcggg aatcaacttg aacaattgca
    25861 gcctttactt caaatgccgt ctgaaaacat tcaaaccatg cttagaaacg aatggcctgc
    25921 tcctaagact ttcctattgt tggctaaatc tggcgtatta cctgcgttgc gcgggaagag
    25981 gcgcagcaag cttgcggtca gagtgcccga tcatacaggt gcaaggcgtt tgtgtcaggc
    26041 attgggtatg cctttggttt ccacttcatg taatcgggcg ggtaaacgcg cgtgtcggac
    26101 ggagagggaa gtcagaaggc agttcggacg cgatgtgtgg attgtcggtg ggagaatcgg
    26161 caggcagaaa tcgccgagtc agattatcga tggggaaaca ggtaaacgtt tgcgttagat
    26221 atggatgctt gagtgaaaac ttttttgcat acacaaagaa aaaatttttt ggatggtatt
    26281 tctctacatg attgttatcg cactgcgata aaggtagaag gacgcaacgt ctgctttgat
    26341 tttgaggacg gttttgtcgt tctggacaat aatccgaaca atcaagcggg caagcatctg
    26401 aaaacagatt tttcccgtgt tgttttaacc catgaaaata gaaacgccga agacgactat
    26461 ttggtcgata tttttgaaga catcgtcttt ttagggaaac gtttgtttac agtcagaaaa
    26521 tttcttgatt tttctgaatt gcttgaaatg atcaatgcac aaggctattt tcttgaattt
    26581 ctgtatctgt atgaatgtat cggtgtcaaa acaagcgatt attttttaga ggctgtgttg
    26641 gttacgggac ggagccggga atgtaaaaaa tgctttatca aaattatgag ggcaagcagt
    26701 tttgtgtatc aatggaacaa tttacgtttg aatagtgaga ttgtatagcg ggtttatatt
    26761 tcggatggaa gccggtttaa aaaaataatg tggataattt cagggaatgg caaaatgtcg
    26821 tctgaaatgc tgatatttca gtattccaga cggcattttt ataataaggc ttatggataa
    26881 atttgtgtat aagttgaaat cagagttttt tcccgcatac ggtttcatat tgtttttcag
    26941 ttaatgtccc ggacaggatg ctcatcgggc ggagggaaga ggctgtgtag ttttgtgtgg
    27001 aaatttccgt gttttttgta tcaaataact gtagcgaact taagcggtat gttttgttgt
    27061 tgcagtggat ttcccactcg gcaatggcag tcttgtatgc gggggtgttg gcaaaacgtt
    27121 cttgtttcag attggtaaca acttttttat cttggaaaat catcagattt ccgttttttc
    27181 tcacactgtc tttattaata tatgtatgaa tattgccgtt tgagattgtg ccgatatttt
    27241 cccatcgggc agacttgtcc ggtgtgctgc aggcggttat caaaataggg agtaaaacga
    27301 aagataggaa gattgggcgc attagggttt ccttaatcat tgaaatccgc tcattcagaa
    27361 agaacgggtt gggccggagg ggtaatgccg tctgtgaaat gaggcaaata tgggaaattg
    27421 atgcagcctt cgtgttttac gtatttatat attccgtatg gaggaatatg ctgcttttga
    27481 agagtgtatt atggatgaac tgccgataac agtaaatcgg tttgatttgt ttccgtcagc
    27541 ttatgccgtc tgaagccctt tcagacggca tttattttga agttctggtt gtttttgcca
    27601 gtgatatttt gatggtttga agctgtcttt tatattcccg gcaatgcggg cagaacagca
    27661 ggtgcatata tatggaaatc ttctcgcccg gggtggtttc ccggtcctga tgtttggaaa
    27721 gaagcagggc aatatcgcgg cattttttca taacgtttac ttcgggtttt cttggttgaa
    27781 ccatttgatt tgcaggcatt ggcgcaatga ttctcgggcg cggtgcataa tggtgtggta
    27841 gttggacgtg ctgataccgc acatttgttg tatttcgttg gatgaaaaac cgagtatttc
    27901 cttcagggtg aatacccgtg cggtgttttc aggcaggttg tataggcagc tttgcagaat
    27961 tttttggaat tcgttgttat ttaatgattt ttccggagtg ttccaatgtt gcggctgccc
    28021 ttccggcgtc caatgcccgt tttgggaaaa atggctttca aatgcttcat ccagcagctc
    28081 gtcatccagt gcggtaaaga ctttcctctg cctcccgatt tgacgtaatg cgtcaataat
    28141 tttgtttttc aatatggcaa aaagccagct gttgacaagt gccctgcctt gaaaactgtc
    28201 gcctgcgctg tatgcggaca gcaatgtttc ctgcactaaa tcttcagcca aatcagggtg
    28261 gtcgggcaac tggagtcgcg caaaatgcag aagcagtttg cgcgactcca ttaattcggc
    28321 atcggtcagg tcgggtagcg gcatagtatc gggaacaagg taattttatg cggatagatg
    28381 gttttggctc ggggaattgc cttccatatt ttcggatatg gcggacaagg aggggatgat
    28441 aatcctttgt tcaatccagc ctgaccattt cccaagcaaa atatttttcc aattatcggc
    28501 tttaggcata aattcgacaa gggtttgggc gagggtgtca aacgaaagcg cggaggaacc
    28561 cattatctcc agcagcatca tatcgaagcc gtccaatgtt tggtacatca catcatcttc
    28621 ggcgtttcgc catattaaca aggctgtttc cgcttcctgc aaatcatggg taacatcgta
    28681 tcgatattgc cggataaagg ccgcagggga gggcgtgtat ttgctgtcat ttgaataatg
    28741 aatgtcggga atatccggaa tttgtgcaac ttctgccagc aattgggtat attcaaaatc
    28801 catcagtgcc aaaatgccgt ctgaaagcgg caggctttgg caatattgta ggaactcgcc
    28861 ggggatttct tgaaaatagg gcgtttgggc acgcgcgtcg cggacgaaac cttctttcag
    28921 acggctccat tctttgctgt caaagtattg ccgcgtttcg gtatagcagc ggtcgataaa
    28981 gctgtggata ttgttgcgta tcaggcggat atagacgttc aatcggtctt gcggcagacc
    29041 gtctgcggct tcgcccccgc gtatggcttg ggagaaacgg tgctggtatt gggcggaggt
    29101 ttcaggctgc acggcggtat tcctttccgg cacgcgtttg ataatcggca attttggcaa
    29161 cttcggcttc gagttcggca aaaggtggga aattgaaatc gcgttccaac agggtgggag
    29221 ggatggtggg aagcttggtg taggcgagtt cgagcaagtc ccaaacggtc ggtaaaaccg
    29281 ccgcgccgtg ggtatcaatc aaaagctcgg gggtttcggc atcgtgtccg gcgatgtgga
    29341 tgtagcacac gcgccctgcg tccacattct ctaaaaaggc ctcaggcgac agcaggccgt
    29401 gattgacggc gttgacgtaa atattgttca catccaaatg aataccgcag tcggcttcac
    29461 gggcaaccgc gttgaggaac tcgacctcgt tcatctcggc aagcggagaa tgcaggtagt
    29521 aggacgtgtt ttccacggcg atgcggcagc ccaaacggtc ttgcacttcg cggatacgcc
    29581 gcgccgtatg atgcaccatt tcttcagtga aaggcagcgg caacaaatcg taaagatgac
    29641 cgccgtcgtg gcagtagctc aaatgatcgg agaaaaacgt gcagtcataa cggcacatca
    29701 tttctttgat gccgtctatc aaatcggtat ccagcggggc ttgtccgccc agcgacatag
    29761 ataatccgtg caacgccagc ggcagccgtt ccgccacacg gtcaaactgt ttgcgcgccc
    29821 tgccgcccat tttcagccag ttttccggtg cggcttcgat aaagcatatc gggctgtttt
    29881 cggaaagcga gagaaagtct tccgccaagt cgcggcggta gcccaagcct gcgtgttgaa
    29941 tcatattttt acccggttcg gatggcacaa aagaataatg tgcttgttta aaaaaatctt
    30001 tgtttgccgt taaaaaagcc gcattttact gaaaaacgcg gcttggtttg aaggggaggg
    30061 attatttaga accgcatttg ccttcgccgc atttgccttc ggcagatttg gctttggctt
    30121 tagatgcttt ggtgtgtttg tgggcttttt ttacagttgc accgcatttg ccctcgccgc
    30181 atttgccttc gcctgcttta gaagcagccg cgccgcacga accttcggca gatttggatg
    30241 cgccgcaaga gccttgggcg gatttttgaa cgcctgttgc gttgcttgcc ggtttgtggg
    30301 cggcaacggc gccggcggcc aaagacaggg ataaagcacc ggcgagtgcg gcagcaatat
    30361 ttttgttcat ttctatttcc ttttcagaat atcaagttaa cgagataagg ttggaatgtt
    30421 taaagtcgga ttccacgact gaatcaatct tgtttcagac ggcatttggg gcaaaaccgt
    30481 tttttcagca gcgtatccag cgaccatccg cccgcaccct ggaaaagcag cgggattaat
    30541 accacgatat aaattaaagc cattttataa ccgttgtcgc agacattgta acccgaaccg
    30601 gcgtgaaccg cagcccaagc gacggcggta acgaccatca gccccaatgc cgacaggcgc
    30661 gttgccaaac ccaaaagcaa cagtacgggc agcaaaagct ccgcatacat ggcgaaattc
    30721 cagtttaacg cgtccggcag caagttgaac gggaatggaa actgatcgtt gatttcggaa
    30781 aaccaattct ccccgttcca tttttgcaaa cccgattcca aaaattcata tgcggcaaac
    30841 agacgcaaaa ccagcatagc ggatgccgtc tgccaagttg ttccgatttt cataagtgtt
    30901 cctgtttttg ttcctgttga taccccgtta gacggatttt gtcggaaaaa ctgacacccg
    30961 tcagctaata atttaacaag ttaatgaata taaaaagaaa gtattttgac ggaaagaaag
    31021 ggaaaaaggc gggatatttg gataacggcg gtgttgtgag ataattcccg ccttggacgg
    31081 tatgccttca aatacaattt ccgtaaagga aaaaaagaaa atattaattt tgatgatgac
    31141 ttaacaaagc ccctttcgct tggctgtcaa aggagattgt taagaaaagc aatctccctt
    31201 tgacggggtg cgatatgtaa aggaatggaa aatatggtaa cgttttcaaa aatcagatca
    31261 tttttggcaa tcgccgccgc cgcgttgctt gccgcctgcg gtacggcggg aaacaatgcc
    31321 gcccgcaagc cggtgcaaac cgccaaaccc gccgcagtgg tcgctttggc actcggtggc
    31381 ggcgcatcta aaggatttgc ccatatagga attgttaagg ttttgaaaga aaacggtatt
    31441 cctgtgaagg tggttaccgg cacatcggca ggttcgatag tcggcagcct tttggcatcg
    31501 ggtatgtcgc ccgaccgcct cgaattggaa gccgagattt taggtaaaac cgatttagtc
    31561 gatttaacct tgtccaccag tggttttatc aaaggcgaaa agctgcaaaa ttacatcaac
    31621 cgaaaagtcg gcggcaggca gattcagcag tttcccatca aatttgccgc cgttgccact
    31681 gattttgaaa ccggcaaggc cgtcgctttc aatcaaggga atgccgggca ggcggttcgt
    31741 gcttccgccg ccattcccaa tgtgttccag ccagtcatca tcggcaggca caaatatgtt
    31801 gacggcggtc tgtcgcagcc cgtgcccgtc agtgccgctc ggcggcaggg ggcgaatttc
    31861 gtgattgccg tcgatatttc cgcacgtccg agcaaaaatg tcggtcaagg tttcttctct
    31921 tatctcgatc agacgctgaa cgtgatgagc gtttccgtgt tgcaaaacga gttggggcag
    31981 gcggatgtgg ttatcaaacc gcaggttttg gatttgggtg cagtcggcgg attcgatcag
    32041 aaaaagcgcg ccatccggtt gggcgaggag gcagcacgtg ccgcattgcc tgaaatcaaa
    32101 cgcaaactgg cggcataccg ttattgacgc tatgccgtct gaacgtattt ccagaaggct
    32161 ttatccatag aaaaaaatac ccgaatcccg tacaatacgc ccctaaaatt ttgaaagaac
    32221 gaaagaatca tggaagccga agtaatcaac cagctcaaca acaccctgaa tgatttggag
    32281 aagcgtagcg aagacatccg cgtttatatg gattatcagg gcaagaaaga ccgattggaa
    32341 gaagtcatcg ggctttccga agaccccgag ctttggaacg acccgaaacg tgcccaagaa
    32401 atcggcaaag agagcaaaat ccttgaaggc atcgtgctga cgctcgacaa catcgcttcg
    32461 ggtatcgaag acaaccgtat gctgattgaa atggccgtcg aagaaaacga cgaagaaggt
    32521 tttgccgccg tgaaggaaga cgtggcgggg ctggaaaaac agatggcgga tttggagttc
    32581 aaacggatgt tcaaccagcc cgccgacccg aacaactgct ttatcgacat caccgccggc
    32641 gcgggcggta ccgaagcgga agactgggcg gggatgctgt tccgtatgta cagccgctat
    32701 gccgagcgca aaggcttcaa aatcgaaatc cttgaggaag acgacggcga aatcgcaggc
    32761 atcaaccgcg ccaccatccg agtggaaggc gaatacgctt atggtttgct gcgtaccgaa
    32821 accggcgttc accgtctggt gcgctactcg ccgtttgatt cgaacaacaa acgccatacc
    32881 tcgtttgcct ccgtgttcgt ttaccctgaa atcgacgatt ccatcgaaat cgaaatcaac
    32941 cccgccgatt tgcgcatcga cacctaccgt gcatcgggtg cgggcggtca gcacatcaac
    33001 aaaaccgact ccgccgtgcg tattacccac gagccgacgg ggattgtggt gcaatgtcaa
    33061 aacgaccgtt ctcaacacgc caacaaagcc gccgcgatgg aaatgttgaa atccaaactg
    33121 tatgaattgg aaatgcgcaa acgcaacgaa gagaaacagg cattggaaga aggtaaatcc
    33181 gatgtgggtt ggggcagcca aatccgttcg tatgttttgg attcctcacg tatcaaagac
    33241 ttgcgtacag gctacgaagt cggcaacacc aaagccgtat tggacggcga cttggacggc
    33301 tttatcgaag ccagcctgaa acagggtgtg tagtttaggc tgtatgccgt ctgaaacctg
    33361 taccgtttca gacggcattt ttgcgtttcg tgttataatc cgcgccgcac acgggacggg
    33421 cagacagtcg ccgcgtatcg cgtaaggcat acggggagga aagtccgggc tccgcagggt
    33481 agaatgccgg ttaacggccg ggcgcggtaa cgcgacggaa agtggaacag aaagcaaaac
    33541 cgccgatggc tgctttggca gcacaggcaa gggtgaaaag gtgcggtaag ggcgcaccgc
    33601 gcatttggta acaatatgcg gcaggccaaa ccccattcgg agcaagacca aacagaacgc
    33661 aatgacgctg cccgccgagc gttcgggtag gttgcttgag cataccggca acggtatgcc
    33721 tagaggaatg actgtccgag acagaacccg gcttaccgcc tgtccggtgt gcatccgaca
    33781 cgcaaatgcc gtctgaaacc tgaaatacgg ttcggacggc atttttattt aggctgtgtt
    33841 tttatattcc gttgatgcag ccctgatgag tcggacaaaa tgccttttac tcaataggac
    33901 cggtttccgc ccaagcggaa ataaacggct atttcccttg caaacagacg gaatcaggcg
    33961 ggtatttaaa tgacaatcgg caggctgcct tccggttggg ggggaaagcc tgtcctgtcg
    34021 ggtggaaacg gtttggtttc attatgcgga aaaatgccgt ctgaagggtt cagacggtat
    34081 cggcgcatcg gggcaggact tagtctgtgc cttcgagcat tttgttcaat ttggggacga
    34141 gcaggagcag caggaagcct gtggctgcgc cgatgtagaa cagcagccgg tagaagccga
    34201 tttcgtcgcc cgcctgatag cctttttcaa acaatacgcc gcccaaagtg aagcctaatg
    34261 aaaaggcaag gaaattaagg gcgaccattt gggttttgaa taaaggcggt gcgattttgg
    34321 tggagatgga cagcgcaatc ggggaaatca tcagttcgcc tatcgtgatg gcgaggacga
    34381 tcagtgcgaa aaccgcaata ggcatcggcg ttccggagga aataaaggga acgaatccca
    34441 aaaacgacgc accggtaaca aataccgcca tagcgaattt cagcggggtt ttgggttgtt
    34501 tgcgccccat ttttgtccac attgccgcca tgagtccgga aaacaggatg acccacaggc
    34561 tttgcataga atctttccag gcgacgggta cggtaaacga accgatggtg cggttgacgg
    34621 tttcgtcgaa atagacggtt gccacggtgt aaatctgaaa ccagacggcc caaaacatac
    34681 agatggtcag gaaaagcggg atgtaggcga tgatgtgccg tttgttgtcg gaactgacgc
    34741 gggggttggt cagcaggcgg gcgaaatagg cgatgacggc aaggatgacg gtagataaga
    34801 ggatgcggga gaaattgtcg aggttgacaa gcccggtttt gatggcggtt gcaagtgcgg
    34861 cgatgagggt gatgccgacg gcggccgcag ttttgccctg tccttttgaa agcggatggg
    34921 ggacggtggg gtggggcagg tttttacgtc ccaaggaata acgccacaag ccgaatgcca
    34981 taccgaccgc cgccgcgccg aaaccataat ggaaaccgat gttttcttga agcagtccgg
    35041 tcaataaggg gcctaagaag ccgccgatat tgatggcgat gtagaaaatg gaaaatcccg
    35101 catcgcgcag cgggcgcatt tcgtcctgtt cgtataatgc gcccaccata gaactggccg
    35161 tagatttcac gccgccgctg cccaatgcga tgaatatcag cccgattaga aggccgtaca
    35221 ggcccggtgc ggcggcaagg acgatgtgcc cgagcattac gacgatgccc gagaggaaga
    35281 gggttttttc cgcaccccat acgcggtcgg caaaccacgc gcccaaaatg gtggacaggt
    35341 acacgctgcc gctgtatgcg ccgacaatgc cgcctgcgag ggttttgtct atgcccaagc
    35401 cgcctttgtc ggcggtgtag tagaggtaaa tcagcaggat gccctgcatt ccgtaaaatg
    35461 aaaaacgttc ccacaattcg atatggaaga gggtggaaag ctggaagggg tggccgaaaa
    35521 atgttttttc tccggtgcgg gcgggatgcc gggacataaa ggttctccta aaagattttc
    35581 tctcaatacc gtctgaaacg gatgcggacg gcggcggtgt ttttttgtat gaaaatatcg
    35641 gtttttaacc gatattttca ttctttgtca aacgacgcgc tgccgttttt cgcgggcgga
    35701 tgtttttata tttgtttcaa ttcaatggat tgtattttag aggacgtgtt ccgatacggc
    35761 gcgggtaaat ccttttctgt caatggctta tccgataggg cggtttttac ttagatggaa
    35821 aatcacttcc ttatatatcc gccacgccga agggcgcatt atggtggatt aactt
//
LOCUS       DAXDXW010000014        34850 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXDXW010000014 DAXDXW010000000
VERSION     DAXDXW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34850
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.014"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(320..967)
                     /gene="adk"
                     /locus_tag="ACFB64_000576"
     CDS             complement(320..967)
                     /gene="adk"
                     /locus_tag="ACFB64_000576"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC1208992.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVG
                     LDAVVEIDVSDSVIVDRMSGRRVHLASGRTYHVTYNPPKTEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQAVEAVKAEVLGALGK"
     gene            1182..2021
                     /gene="htpX"
                     /locus_tag="ACFB64_000577"
     CDS             1182..2021
                     /gene="htpX"
                     /locus_tag="ACFB64_000577"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC1208993.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(2257..3555)
                     /locus_tag="ACFB64_000578"
     CDS             complement(2257..3555)
                     /locus_tag="ACFB64_000578"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC1208994.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHEGLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQYLHNAGPVKAEDVMAVIEKVAPRIAPMIADVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPGGGKTDILPCGSDAVETCKPIYETMPGWRESTVGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(3660..4811)
                     /locus_tag="ACFB64_000579"
     CDS             complement(3660..4811)
                     /locus_tag="ACFB64_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC1208995.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLPNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGSVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDNEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAVERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQIVKR"
     gene            5146..6525
                     /gene="norM"
                     /locus_tag="ACFB64_000580"
     CDS             5146..6525
                     /gene="norM"
                     /locus_tag="ACFB64_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HFC1208996.1"
                     /translation="MLLDLDRFSFSVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTGEAGETGRQGIW
                     FGLILGIFGMILMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGVATMAVFWFSALALW
                     IYIAKEKFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVSGWVL
                     AVITVLSLVLFRSPLASMYNDDPAVLSIASTVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAVALVWCLEKYSM
                     ELVKSHEAV"
     gene            6569..7609
                     /gene="murB"
                     /locus_tag="ACFB64_000581"
     CDS             6569..7609
                     /gene="murB"
                     /locus_tag="ACFB64_000581"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC1208997.1"
                     /translation="MQPIRYRTDLTPYNTFGLHAQARAFIALKHADELRDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYDGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAKVAELSAGREATAKDVS
                     DAVSAIRNSKLPDPKVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLANKNNASSDDIWKLAQHVCNTVFTRFQVELH
                     AEPNWLPASFSL"
     gene            7621..8271
                     /locus_tag="ACFB64_000582"
     CDS             7621..8271
                     /locus_tag="ACFB64_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC1208998.1"
                     /translation="MPVTRIAKTNTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVKLLTQLNGLNVINADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGVRRTLSLLRPYLLPEHRAEYDRKISNGNP"
     gene            8326..9102
                     /locus_tag="ACFB64_000583"
     CDS             8326..9102
                     /locus_tag="ACFB64_000583"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC1208999.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYRHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLNRTDTFQTALWRHHADKPVWFFLLPPSFLAHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDKARECNETAAPDPQTESARQILAAEQHLLDNGVPNIDILRLGG
                     LYCAERHPVSRLVQKQNIQGGNRPVNIVHRDIAVENLFQTASNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFSPDDSVGKIIRTVCDNGLSL"
     gene            9152..9718
                     /locus_tag="ACFB64_000584"
     CDS             9152..9718
                     /locus_tag="ACFB64_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC1209000.1"
                     /translation="MSALLPIINRLILQSPDSRSELTSFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAIRKILQGGEPGAGDIRLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQAGIGSRATDIGHGIKQIGRNIAEQIGGFSREPESANTGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            9738..10628
                     /locus_tag="ACFB64_000585"
     CDS             9738..10628
                     /locus_tag="ACFB64_000585"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC1209001.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGV
                     RECCIYTQDTDGCHIVNKTELGQYCDLVAVLGGDGTFLSAAREITPRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERALALNDAVLSRGG
                     AGQMIEFEVFVNQEFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQSFIDVQNLDRIIIRRYHNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            10653..11411
                     /locus_tag="ACFB64_000586"
     CDS             10653..11411
                     /locus_tag="ACFB64_000586"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC1209002.1"
                     /translation="MNSKISSDHTEDTIRLSKRMAQLGLCSRREADGHIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NCWEGDTSRISFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGDKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(11466..12131)
                     /locus_tag="ACFB64_000587"
     CDS             complement(11466..12131)
                     /locus_tag="ACFB64_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC1209003.1"
                     /translation="MTVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWRFVVVQNPEIRQAIKLFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAVEKSLARYQAFQADDIKILDDSRALFDWCCRHTYIALANMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIVTKARRPLEETV
                     IWA"
     gene            complement(12328..13101)
                     /gene="folE2"
                     /locus_tag="ACFB64_000588"
     CDS             complement(12328..13101)
                     /gene="folE2"
                     /locus_tag="ACFB64_000588"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC1209004.1"
                     /translation="MNAIADVQSSRDLRNLPINQVGIKDLRFPITLKTAEGTQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHTEVLDFAQLHRLTAEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVSLTGEMKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTSDAEVGIEEVIDYVETQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            13304..14461
                     /locus_tag="ACFB64_000589"
     CDS             13304..14461
                     /locus_tag="ACFB64_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC1209005.1"
                     /translation="MKFPTRAIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHNVKLVWLEMPSNPLLRLVDIKALAA
                     KAKAADALVGIDNTFATPYLQQPLDMGCDFAFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPTDCWLVLRGIKTLALRMEAHCKNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYALAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMTSSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIRVGLLRFSIGIEDVDDIWNDISAALDTTL"
     gene            complement(14525..15526)
                     /gene="hemB"
                     /locus_tag="ACFB64_000590"
     CDS             complement(14525..15526)
                     /gene="hemB"
                     /locus_tag="ACFB64_000590"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC1209006.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHMLTADDLIYPVFVLE
                     GAAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTGRAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGTQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(15710..15994)
                     /gene="yhbY"
                     /locus_tag="ACFB64_000591"
     CDS             complement(15710..15994)
                     /gene="yhbY"
                     /locus_tag="ACFB64_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC1209007.1"
                     /translation="MADTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICNTLCEAVDAQPVRHIGKLLVLWRKNIEA"
     gene            16104..16724
                     /locus_tag="ACFB64_000592"
     CDS             16104..16724
                     /locus_tag="ACFB64_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC1209008.1"
                     /translation="MAVRSKSSKAWLHEHINDHYVHMAQKDGYRARAAYKLLEINEKD
                     KIIKPGTVLADLGSAPGSWSQVAAKLTGTSGTVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            16788..18755
                     /gene="ftsH"
                     /locus_tag="ACFB64_000593"
     CDS             16788..18755
                     /gene="ftsH"
                     /locus_tag="ACFB64_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC1209009.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIRQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREKTEAPAPADTASTESGQQPENKA"
     gene            18977..19408
                     /locus_tag="ACFB64_000594"
     CDS             18977..19408
                     /locus_tag="ACFB64_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC1209010.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMRVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            19401..19679
                     /locus_tag="ACFB64_000595"
     CDS             19401..19679
                     /locus_tag="ACFB64_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC1209011.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRTAALQSGLDGIFENLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            19732..20310
                     /gene="pth"
                     /locus_tag="ACFB64_000596"
     CDS             19732..20310
                     /gene="pth"
                     /locus_tag="ACFB64_000596"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC1209012.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSAEHR
                     RQIDDAVAKSLQAVPDIISGKWEEATRFLHSK"
     gene            20371..20577
                     /locus_tag="ACFB64_000597"
     CDS             20371..20577
                     /locus_tag="ACFB64_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC1209013.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            20574..20936
                     /locus_tag="ACFB64_000598"
     CDS             20574..20936
                     /locus_tag="ACFB64_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC1209014.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQILLLAKAFKTGKLPIPYRSLIAFAFCFAVGSECAQAWFTATRTGSLGDVLAD
                     LTGAALALFAARSACRPD"
     gene            21165..22580
                     /locus_tag="ACFB64_000599"
     CDS             21165..22580
                     /locus_tag="ACFB64_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC1209015.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITITALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFGSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIAASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            22700..23209
                     /locus_tag="ACFB64_000600"
     CDS             22700..23209
                     /locus_tag="ACFB64_000600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC1209016.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSAGAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIESVSTKRHGYHDDVPTEP
                     VIIIKAEAV"
     gene            23377..24759
                     /locus_tag="ACFB64_000601"
     CDS             23377..24759
                     /locus_tag="ACFB64_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC1209017.1"
                     /translation="MASITRDIFKAYDIRGIVGKTLTDDAAYFIGRAIAAKAAEKGIA
                     RIAIGRDGRLSGPELMEHIQRGLTDSGIGVLNVGMVTTPMLYFAAVNECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGSVTEKDISGAYHDHI
                     VGHVKLKRPINIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPE
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLNRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSAMKKTGALVAGEMSGHVFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLDNLPQSISTPELNISLPEGSNGHQVIEELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNRFKAVI
                     ESNPHLIWPL"
     gene            complement(24815..25570)
                     /locus_tag="ACFB64_000602"
     CDS             complement(24815..25570)
                     /locus_tag="ACFB64_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC1209018.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNARPLRIDFSKKTSKHDILALRRKSGMVFQQYNLFPHKTVL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLDAMKELAREGWTMVVVTHEIKFTLEVATNVVVMDG
                     GVIVEQGSPKELFDHLKHERTRRFLSQIQSAKI"
     gene            complement(25580..26296)
                     /locus_tag="ACFB64_000603"
     CDS             complement(25580..26296)
                     /locus_tag="ACFB64_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC1209019.1"
                     /translation="MFNNFLASLPFMTETRADMLISAFWPMVKAGFTVSLPLAIASFV
                     IGMIIAVAVALVRIMPSGGIFQKCLLKLVEFYISVVRGTPLLVQLVIVFYGLPSVGIY
                     INPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(26286..27113)
                     /locus_tag="ACFB64_000604"
     CDS             complement(26286..27113)
                     /locus_tag="ACFB64_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC1209020.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAISGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSEPYSWSGAVLVARNDSNIKSIADIKGVKTA
                     QSLTSNYGEKAKAAGAQLVPVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            27398..28306
                     /locus_tag="ACFB64_000605"
     CDS             27398..28306
                     /locus_tag="ACFB64_000605"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC1209021.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFPEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDETIREVAI
                     IPEGLPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRSKETDDEFSRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            28383..31985
                     /gene="recB"
                     /locus_tag="ACFB64_000606"
     CDS             28383..31985
                     /gene="recB"
                     /locus_tag="ACFB64_000606"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC1209022.1"
                     /translation="MSAPIRAFDPLTAPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKSVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDDTLSDGIAAYCAE
                     HHEGDTFLPALLEQALQKESRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSGDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRDAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHERLLKLSSDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNSSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     PARAVAENWEIALIDEFQDTDPLQYEIFQKIFIARNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQLRWLHENDNEKANKDVLRRRAADYCADEIAHALNEASGGRLNFKGRPLQ
                     SGDIAVLVRTHNEAVMISAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDIWQKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRAELLAK
                     AQLSESEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRRTYACEKDGITMLKRNWRRVADNAPAGTDFAFTEDAPPPAAYRGNADQAAE
                     FAANSIPERGFRFVRHTSFTALSRQTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAEQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYGLSDIPPGCRRPEMGFTLHTEDFGLKRLRDWLARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICIIDYKSNHLSAYTRQAMDEAVAHQHYYL
                     QALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK"
     gene            32024..32383
                     /locus_tag="ACFB64_000607"
     CDS             32024..32383
                     /locus_tag="ACFB64_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC1209023.1"
                     /translation="MNIKQLITAALIASAAFATQAAPQKPVSAAQTAQHSAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIYEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            32508..32987
                     /locus_tag="ACFB64_000608"
     CDS             32508..32987
                     /locus_tag="ACFB64_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC1209024.1"
                     /translation="MKHILPLIAASALCISTASAHPADKPPTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRTAYTDTRALIVGSRISFDEVANTLANAEKLIQKTVGE"
     gene            complement(33262..34641)
                     /gene="radA"
                     /locus_tag="ACFB64_000609"
     CDS             complement(33262..34641)
                     /gene="radA"
                     /locus_tag="ACFB64_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC1209025.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESFAAPEPK
                     NARFQSWAADASTVQSLSAVTAAEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRSQRLELHAEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLSVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIHGVSSLQEA
                     IDICRDSRE"
BASE COUNT         8283 a        10348 c         8780 g         7439 t
ORIGIN      
        1 caacgtaaat tcaaaccgta ggccgggcat tgatgtccga cctacgcgga ctcaagcccc
       61 cgacaaactg tttcggataa atttgaaccg gtacatcagc cgtcattccc gcgcgggcgg
      121 gaatccattg ataaaactca gtttatcaga tttaaacagc gattgcccaa attcagaaat
      181 ggattcccgc ctgcgcggga atgacgccga atgattattt ttcggttgca acacgctgtt
      241 tatcaaaaac aggtcgtctg aaaacctgat tttctgtttt cagagtgcga agcctgcccg
      301 tgggtacgac cttttcgatt tatttgccca atgcgcccaa tacttcggct ttcacagctt
      361 ctactgcttg ggtaccgtcg actttgatgt atttcggcgc gtgttcgcct tccagcttgc
      421 tgtaaaaatc aaccaaaact tcggtttgct cgtggtaaac ggcaagacgt tttttcacgg
      481 tttcttcttt gtcgtcgtcg cgctgaatca aatcttcgcc ggttacgtcg tctttgcctt
      541 cggttttagg cggattgtag gtaacgtggt aagtacggcc ggaagccaaa tgcacgcggc
      601 ggccgctcat gcggtcgaca atcacgctgt cggatacgtc gatttcaacg accgcgtcca
      661 aacccacgcc tgcttccacc atcgcttcgg cttgcgccaa tgtacgcggg aaaccgtcga
      721 acaggaaacc gtttttgcag tcgtcttgcg cgatgcgttc tttgaccatg ccgataatga
      781 tgtcgtcgcg caccaaaccg ccttcatcga tgattttttt cgcttccaaa cccaagggcg
      841 tgcctgcctt aatcgcggca cggagcatgt cgccggtaga gatttgcgga atgccgaacg
      901 cggcggtgat gaattgcgcc tgagtgcctt tgcccgcgcc cggtgcgcct aaaagtaatg
      961 ctttcatgaa gtgatccttt taaatgtgtt tattggaatc aaacagatgg tttacgattc
     1021 tacacggctt ggcgcaatag ttcaaaaacc aactgcacaa actgccattt gcaagcaaat
     1081 gttttgcata cctttgccga gcctgatatt cacaaggcgg caactttttg ggacaatccc
     1141 caaagtttca aaacatcaca ccctaccaaa aaggaacatc cgtgaaacgc atttttctgt
     1201 ttttggctac caatatcgct gttttggtcg taatcaacat tgttttggcg gttctgggca
     1261 tcaacagccg gggcggcgcg ggcagcctgt tggcgtattc cgccgtcgtc ggcttcactg
     1321 gttcgattat ttcgctgctg atgtccaaat ttatcgccaa acaatcggtc ggtgcggaag
     1381 tcatcgacac gccgcgcacc gaagaagaag cctggcttct gaacactgtc gaagcccaag
     1441 cgcggcaatg gaacctgaaa acgccagaag tcgccatcta ccactccccc gaacccaatg
     1501 cctttgccac gggcgcatcg agaaacagct ccctgatcgc cgtcagcacc ggtttgctcg
     1561 accatatgac gcgcgacgaa gtggaagccg tgttggcgca cgaaatggcg cacgtcggca
     1621 acggcgacat ggttacgctg acgctgattc aaggcgtggt caataccttt gtcgtgttcc
     1681 tgtcgcgcat tattgccaac ctgattgccc gaaacaacga cggcagccag tcccagggaa
     1741 cttatttcct agtcagcatg gtattccaaa tcctgttcgg cttccttgcc agcctgattg
     1801 tcatgtggtt cagccgccaa cgcgaatacc gcgccgacgc gggcgcggca aaactggtcg
     1861 gcgcaccgaa aatgatttcc gccctgcaaa ggcttaaagg caacccggtc gatttgcccg
     1921 aagaaatgaa cgcaatgggc atcgccggag atacgcgcga ctccctgctc agcacccacc
     1981 cttcgctgga caaccgaatc gcccgcctca aatcgcttta aaccgatttg aaacgacaaa
     2041 aaaccgtacg cacaagcagt acggtttttt cgtatatgcc gactgaaaac atcaggtttc
     2101 cataccggca ccaaccgtat ccatttccgc accggatacg ccgcattgat atatccccga
     2161 caaaccgtgt ccggacagaa aatatcagat aaaacagcgg taaaaatgcc gtctgaaacc
     2221 aattagggct tcagacggca tttttactgc aaaaccttat gcgaacggat gatgcagcac
     2281 aatcgtttct tcgcggtcgg ggccggtgga gacgatggcg accggcgcgc cgcagacttc
     2341 ttcaatccgt ttcaaatatg ctttggcatt ggcaggcaat gcgtcgtagc ttttcacgcc
     2401 gacagtcgat tcgcgccagc cgggcatggt ttcgtaaatc ggtttgcagg tttccaccgc
     2461 atccgaaccg caaggcagga tgtcggtttt gccgccgcct ggcaattcgt agccgacgca
     2521 gatattgatg gtttcaacgc cgtccattac atcgagtttg gtaatgcaca taccggaaat
     2581 gccgttgatt tggatggagc gtttcagggc ggcggcatca aaccagccgc agcggcgcgc
     2641 gcggccggtt accgaaccga attcgtgtcc gcgctccgcc aaacctgcgc ctacttcgtc
     2701 gaacaattcg gtcgggaacg gacccgaacc gacgcgcgtg gtataggctt tgacgatgcc
     2761 caaaacataa tccagcattt gaggacctac gcccgcgcct gccgaagccg cgccggcgag
     2821 acagttggac gaggtaacga aggggtaagt gccgtagtcg atgtccaaca acgcaccttg
     2881 cgcgccttca aacagcagtt tttcgccgtt tttgtttttt tcgttcaaca cgcgggacac
     2941 gtcggcaatc atcggcgcaa tgcgcggcgc gactttttcg ataaccgcca tcacgtcttc
     3001 cgctttaacc ggtccggcgt tgtgcaggta ttggagttgg acgttgtaat aggcaaggac
     3061 ggcatccagt ttttcacgca gtttttcagg atgcagcaaa tcggcggcgc gaatggcgcg
     3121 gcgtgccact ttgtcttcgt aggcagggcc gatgccgcgg ccggtcgtgc cgattttgcc
     3181 tttgccgcgc gatgcttcgc gggcttggtc gagcgcgatg tggtaaggca ggatcagcgg
     3241 gcaggtcggc gcgattttca gacggccttc gacgtttttc acgcctgccg cgttcaactc
     3301 gtcaatttcg cccaacaggg cttcggggga aacgacaacg cccgaaccga tgaagcaatc
     3361 caatccttcg tgcaggatac cgctcggaat caggcgcaaa atggtttttt tgccgccgac
     3421 gaccaaggta tggcccgcat tgtggccgcc ttggaagcgc acgacgccgc cggcttcttc
     3481 cgccagccag tcaacgattt tacctttacc ctcgtcgccc cactgtgcgc cgattactac
     3541 aacattttta gccatagcca tataacctat cgatattaaa aattattgcg gaaaatcccc
     3601 aaaccggtgc ggctttgcgc cccgccccgg tttgcctttc atccgctatg aacgggtatt
     3661 tagcgtttca cgatctgcca aacgccgtcc gtctttttca gacggcctgc aagctcttcc
     3721 gaaacattgt gtccgatacc gtagtcaatc acgacacact gcccttgttc acgcaaggct
     3781 tcgaccgctt cgcgcgccgc ttctgcatct tccgcatcga ccaacacggc gggctgccgt
     3841 tcgacggcgg gcaaacgccc gataaagctg cgtaagtcga aactgaatcc cgttgccggg
     3901 cgcgccctgc cgaaatatcc gcccaatccg tcataacgcc cgccgcgcgc gaccgcgtcg
     3961 tggaaatcgg cggcataggc ggcatacagc aagcccgtgt ggtaattgtc gacgcgcagc
     4021 tcggacaagt cgatatggat ttcattatcg gggaatgcgt cgcacaccgc ctgcaattcg
     4081 tccaacgcgc cgccgaccgc cgacaaatcc ggcaaccgtc cgcgcgcgtc ggacaacact
     4141 tcacgcccgc cgtacaggcg cggcagcaac gagaatgctt ttgcccacat accgtccagc
     4201 ttccaagcct tgacctgcgc ttcgaccgac ccggtatctt tatcctgcat caaggcaagc
     4261 agcgttgcgg actgccccgc atccaaatgc gccgcatcgg acaaggcgcg gaatatgccg
     4321 atatgcccca gcgaaagcag cactttgccc atatcggcaa ttttcatgct tttcagcata
     4381 aggtctatca gctcgatgtc gccacggatg tcggcaaaac cgtacatttc cgcccctgcc
     4441 tgcaagggtt cgcgcatatt cggcagaccg tcgggctgcg cgtgcaacac cggaccggca
     4501 taacacaacc ggttaatccc ttggttggcg gacaaaagat gggcatcgat acgcgccacc
     4561 tgcggcgtga tgtcggcgcg tatgcccaac tgcctgccgc tgagcctgtc cgttaccaaa
     4621 atggttttca gtgaaagccc cgcatcgata tgcgtcagca gggaatgtgc gtactccatc
     4681 agcggaggct gtaccagttc ataaccgtgt acgcggaaca gtgccaacaa ctgctcgcgc
     4741 gcgctttcaa gctgccgcgc gttcgtgggc agtacgtcgg cgatatgttc cggaagctgc
     4801 catgtctgca tgagaatctg ccttctgttt tatcctgtaa acacaaaaga ctgcccgatc
     4861 cgcaatcaga cggcatcttt gccgcaacgc atcacgccgc tgtcaaaacg gcgatgcttc
     4921 cttccgattc agaatccgaa cagcccctgt ccgacggctc aatgccgtca agtcgttaaa
     4981 accaaacttt accataaaat acacacaatc tgaatatact tttatacccc gtatccgccg
     5041 tctgacggca cggtattttt tactatataa tgtgcaccat tatttctcat cacttcccgc
     5101 tgccgttttt ttgtttcaga cggcattttt attgactgaa cacttatgct gctcgacctc
     5161 gaccgctttt ccttttccgt cttcctgaaa gaaatccgcc tgctgaccgc ccttgccctg
     5221 cccatgctgt tggcgcaggt cgcgcaggtg ggcatcggtt tcgtcgatac cgtgatggcg
     5281 ggcggtgcgg gcaaggaaga tttggcggcg gtggctttgg gcagcagcgc gtttgccacg
     5341 gtttatatta cctttatggg cattatggcg gcgctgaacc cgatgattgc ccagctttac
     5401 ggcgcgggta aaaccggtga agcaggcgaa acggggcggc aggggatttg gttcgggctg
     5461 attttgggga ttttcggcat gattttgatg tgggcggcga ttacgccgtt ccgcaactgg
     5521 ctgactttga gcgattatgt ggaaggcaca atggcgcagt atatgctgtt caccagcttg
     5581 gcgatgccgg cggcaatggt acaccgcgca ctgcacgcct acgcttccag cctgaaccgc
     5641 ccgcgcctga ttatgttggt cagctttgcg gcgtttgtgt tgaacgtgcc gctgaactat
     5701 attttcgttt acggcaaatt cggtatgccc gctttgggtg gcgcaggttg cggcgtggcg
     5761 acaatggcgg tgttttggtt cagcgcgctg gcattgtgga tttatatcgc caaggaaaaa
     5821 ttcttccgcc cgttcggact gacagcgaaa ttcggcaaac cggattgggc ggtgttcaaa
     5881 cagatttgga aaatcggcgc gcccatcggg ctgtcttatt ttttggaagc cagcgcgttt
     5941 tcgtttatcg tgtttttgat tgcgcctttc ggcgaggatt atgtggcggc gcagcaggtc
     6001 ggcatcagtt tgtcggggat tctctatatg attccgcaaa gcgtcggctc ggcagggacg
     6061 gtgcgcatcg gcttttcgct tgggcggcgc gaattttcgc gggcgcgtta tatttcagga
     6121 gtatcgctgg tgtcgggctg ggtgctcgcc gtgattaccg tgctttcctt ggtattattc
     6181 cgttcgccgc tggcaagcat gtacaacgat gatccggcag ttttaagcat tgcctccacc
     6241 gtcctgctgt tcgccggcct gttccaaccg gcagacttta cccaatgtat cgcgtcctat
     6301 gccctgcgcg gctacaaagt caccaaggtg ccgatgttca tccacgccgc cgccttctgg
     6361 ggctgcggcc tgctgccggg ctatctgctc gcctaccgtt tcgatatggg catttacggc
     6421 ttctggacgg cattgattgc ctcgctcacc atcgcagccg tcgccttggt gtggtgcttg
     6481 gaaaaataca gtatggagtt ggtcaaatca cacgaggccg tctgaaaacg cagcacactt
     6541 caattcaaat acagttaaag ctcaaaccat gcaacccatc cgataccgaa ccgacctcac
     6601 cccctacaac actttcggcc tgcacgccca agcccgggcc tttatcgcgc ttaaacacgc
     6661 cgacgaattg cgcgacatcg tccgactgcc cgagttcgac cgcgatactg ttttatggct
     6721 gggcggcggc agcaacatcc ttttgatgca ggattacgac ggcttggtcg tccatatgga
     6781 aaacaagggc atacgcgaga ttgcgcgctc agacggcatg gttctgattg aagcgcaggc
     6841 gggcgaaatt tggcacgatt tcgtgttgca caccgtcgcg ctgggtttga gcggtttgga
     6901 aaacctgagc ctgattccgg gtacggtcgg cgcgtcgccc gtgcagaaca tcggcgcata
     6961 cggcgtggag gcgaaagatg tgattcacag cgtgcgctgc ttcgatttgg atacggaaac
     7021 ctttgtaacc ctttccaatg ccgactgccg cttcgcctac cgcgaaagcc tgttcaagca
     7081 ggaaggcaaa gggcgttatg tgattgtttc ggtcgtattt gcattaaaaa cgcattttgt
     7141 gccgaacttg ggctacggcg atttggcggc gaaggttgcc gaattgagtg caggtaggga
     7201 ggcgacggcg aaagacgttt ccgatgcggt atccgccatc cgcaacagca aattacccga
     7261 cccgaaagtt ttgggcaatg tcggcagttt ctttaaaaac cccgtcgtca gcgcggaaaa
     7321 agccgccacc ctgttgcagc ggcatcccga tatgccgcgc tatccgcagc ccgacggttc
     7381 ggtcaaactc gccgccggct ggctgatcga ccaatgccgt ctgaagggct tccaaatcgg
     7441 cggtgcggcg gtacatgacc ggcaggctct ggttttggcg aacaaaaaca atgcctcttc
     7501 agacgacatc tggaaactgg cgcaacacgt ctgcaataca gtatttactc gatttcaggt
     7561 agaattacac gccgaaccca attggctgcc cgcttcgttc agcctgtaaa tccaaggagt
     7621 atgcccgtga cccgcattgc aaaaaccaat acttacaccc gcatcatcga cgccagcctt
     7681 gcgcttttca acgaggaagg cgagcgcaac atcagcacca accatattgc cgcccacttg
     7741 ggcatcagtc cgggcaatct ctattaccac ttccgcaaca aagacgaaat catcgtccaa
     7801 ctgttcaaac gttacagcga agccctgctg gaatacctca acgaagccgt gttgccctct
     7861 gatgtggaag actctatcaa ctatatggca ggcatttacg atgtcatgtg ggaataccgc
     7921 ttcctcttta gcgacgtaaa caccctactt gcgcgcagtg ccgaattgtt gggcgaacac
     7981 aataccttta cccaagccaa agtctcgccg cttctagtca aactgctgac ccagctcaac
     8041 gggctgaacg tcatcaacgc cgaccaaacc gctatgaacg acctcgccgt caatatgtgg
     8101 atggttacga aatactggtt cgacttcgac agttccctgc gcggccgtac caagctgacc
     8161 gaagactcca aagcacgcgg cgtccgtcgt accttaagcc tccttcgccc ctatcttttg
     8221 cccgaacacc gcgcggaata cgaccgcaaa atcagcaacg gcaacccgta aacccataac
     8281 gtccgaatca gccttcagac ggcacttacc cgacggtatc agcgaatgcg cgcagtcccc
     8341 ccgccccata tctccatcac aggcctaggt tacctcggcc tgccgctggc acaaaagttt
     8401 taccgacacg gcagccgcgt cgccgccata aaacgcagcc tgacttcgga cgacatcaac
     8461 ctgcccatac acctcgacac cttcgacctc aaccgtaccg atacctttca gacggcattg
     8521 tggcggcatc acgccgacaa acccgtctgg ttcttcctgc tgccgccctc ctttttggcg
     8581 cattacgccg acaccgtcaa acaatgggca gagcttgccc gggcgtgcaa cgtgcaacac
     8641 ctgattttca caagcagcac aagcgtttac ggcgataaag cgcgagaatg caacgaaacc
     8701 gccgcacccg atccgcaaac cgagtccgcc cgacaaatcc ttgccgccga acaacacctg
     8761 ctcgacaacg gcgttccgaa catcgacatc ctgcggctgg gcgggcttta ttgcgccgaa
     8821 cgccatcccg tcagccgcct cgtgcaaaag caaaacatcc aaggcggcaa ccggcctgtc
     8881 aatatcgtcc accgcgacat cgccgtcgaa aacctgtttc agacggcatc caaccccggc
     8941 ggcaggcggc tgaaaaacat tatcgaaccg cgccacccga cacgccgcga attttatacg
     9001 gaagaagccg ccaaactcgg cctgcccgcg ccggatttct cacccgacga cagcgtgggc
     9061 aaaatcatcc gtaccgtttg cgataacggc ttaagcctgt aaaataagcg gcaacagcaa
     9121 caacaggcat tctccgcccc aatcagaaaa tatgtccgcc ctcctcccca tcatcaaccg
     9181 cctgattctg caaagcccgg acagccgctc ggaacttacc tcctttgcag gcaaaacact
     9241 gaccctgaac attgccgggc tgaaactggc gggacgcatc acagaagacg gtttgctctc
     9301 ggcgggaaac ggctttgcag acaccgaaat taccttccgc aacagcgcga tacggaaaat
     9361 cctccaaggc ggcgaacccg gggctggcga catcaggctc gaaggcgacc tcatcctcgg
     9421 catcgcggta ctgtccctgc tcggcagcct gcgttcccgc gcatcggacg aattggcacg
     9481 gattttcggc acgcaggcag gcatcggcag ccgtgccacc gacatcggac acggcatcaa
     9541 acaaatcggc aggaacatcg ccgaacaaat cggcggattt tcccgcgaac ccgagtccgc
     9601 aaacaccggc aacgaagccc ttgccgactg cctcgacgaa ataagcagac tgcgcgacgg
     9661 cgtggaacgc ctcaacgaac gcctcgacag gctcgaacgc gacatttgga tagactaacc
     9721 ttcagacggc atccgacatg aacagccctt ttcacaacat cggtatcgta acccgcccca
     9781 acacgcccga catccaagat accgcacaca cgctgattac ctttttgaag cagcacggct
     9841 ttaccgtcta tctcgacgaa gtcggcgtaa gggaatgctg catctatacc caagacaccg
     9901 acggctgcca tatcgtcaac aagaccgaac tggggcaata ctgcgacctg gtcgccgtct
     9961 taggcggaga cggcaccttt ctctctgccg cccgcgaaat taccccccgc gccgttccga
    10021 ttatcggcat caaccaaggt catttgggct ttcttaccca aattccccgc gaatatatga
    10081 cggacaagct attgcccgtt ttagaaggaa aataccttgc cgaagagcgc atcctgattg
    10141 aagccgcact gatccgcgaa ggcaaaaccg ccgaacgcgc cctcgccctc aacgatgccg
    10201 tcctctcccg cggcggtgcc ggacagatga ttgaatttga agtcttcgtc aatcaggaat
    10261 tcgtctatac acaacgttcg gacgggctga ttgtctccac ccccaccgga tcgaccgcct
    10321 attcgcttgc cgccggcggc ccaatcatgc aggcaggatt acacgccttc acgctcgtcc
    10381 ccatctgccc gcaatctatg actaaccgcc ccatcgccat tccagacacg tccgaaatcg
    10441 aaatcctcgt tacccaaggc ggcgacgcgc gcgttcattt cgacggacaa tccttcatcg
    10501 acgtgcaaaa cctcgaccgc atcatcatcc gccgctacca caatccctta cgcatcctac
    10561 acccgaccga ctaccaatat ttcaaaaccc tgcgccaaaa actgcactgg ggcgagcaat
    10621 tagtctaagc cggccttcac ccgaaagtac ccatgaattc caaaatttcc tccgaccata
    10681 cagaagatac catacgcctc tccaaacgta tggcacaact ggggctttgt tcgcgccgcg
    10741 aagccgacgg ccatatcgaa cagggctggg taacggtcaa cggcaaaacc gccgtactcg
    10801 gtcagaaagt ttcaccggca gaccgtatcg aactgaacaa gaaagcccac gaacagcagg
    10861 cggcacgcat taccatcctg ctgaacaaac ccgtcggcta tgtcagcgca caagcggaaa
    10921 aaggctataa atccgccgcc gaactgatta cccctgaaaa ttgctgggaa ggcgacacca
    10981 gccgcatcag tttcgatccg aaacacaaaa tcggtcttgc ccccgccggc aggctcgata
    11041 tcgattcagt cggattgctg gtgttgacgc aagacggccg tatcgccaag cagcttatcg
    11101 gcgaaaacag cggcagtgaa aaagaatatt tggtgcgcgt gcgcggcaaa ttggacgaaa
    11161 aaggacttgc cttactgaat cacggattga gtttggacgg tgataaactg cgtcccgcca
    11221 aagtagaatg gcaaaatgaa gaccaactgc gcttcgtgtt gaaacagggt aaaaagcggc
    11281 aaatccgccg tatgtgcgaa ctggtcggac tgcgcgtcgt cgggctgaaa cgcatccgca
    11341 tgggcaaggt caaactcggc aggctgccgc ccggcaaatg gcgttatctc gctcccggcg
    11401 aatcgtttta aataagcatt aaaaaatgcc gtctgaaaat ccttgcgttt tcagacggca
    11461 ctgccttatg cccaaataac ggtttcttcc aagggcctgc gcgctttcgt gacgatttcc
    11521 tgaacccggt agccgaatgt cgcggcaacg gacacgcccc attctgccgc atcgaacaaa
    11581 ccgaactgcc cggacaatac gcgttccata tcggcatagt tgaaaccttc caccgggcag
    11641 gaatcgatac ccgccatcgc cgcacccgtc atcatgttgg ctaacgcgat ataggtgtgg
    11701 cggcagcacc agtcaaacaa ggcgcgcgaa tcgtccaaaa tcttgatgtc gtcagcttga
    11761 aacgcctgat acctcgccaa agatttttct acggcatccg gttcggtaac gccgcgccgt
    11821 ttgagacttt ccaacataaa cgggctgtcg aagcgggcat ttttcttcgc caaaaacacc
    11881 accaaatgac tggcggtatc caaagcatcc gccatacccc aagaaaacag cttgattgcc
    11941 tgtcggattt cggggttttg aaccacaaca aaccgccaag gctccgaacc gaccgaactg
    12001 ggcgacaaac gcccgagttc taaaataaac tgaaaatcct cggcactgat tttacgtgcc
    12061 gcatcgtaat gccggcacga tttgcggttt ttaaatgcgg atagaacctg ctctttgctt
    12121 aatactgtca tcgtgcatcc ttttattact ttgattgcaa attgatttca aaccgatacg
    12181 gcactgccgc accctataag ccgtccccgc catcaagctt cacccggatt ttacccatct
    12241 ctctacggct gggaaaacaa agcggcggca gattcgggaa taactcccca atgcctttca
    12301 gacggcattt ggacatcgca aacgcgccta cgggtaggcg atataggcat aagccgaatg
    12361 gttgtggata gactcgaaat tctcgctctc gacgacgaaa ctcttgatgc gtttgtcggc
    12421 aatcagcgaa gtagcgacat cgcgcaccat atcttccacg aatttcgggt tttcgtaggc
    12481 cttttcggta acgtattttt catcggggcg tttgagcagg ccgtagagtt ggcagctcgc
    12541 ctgcgtttcc acataatcga taacttcctc gataccgact tcggcatcgg aagtcaggct
    12601 gacggtaacg tgcgaacgct gattatgcgc gccatattgg gaaatttctt tggaacacgg
    12661 gcaaagcgag gttacgggaa tcatgacctt catactgtgg ccgtatgccc cgtctttcat
    12721 ttcacccgtg aggctgacat cataatccag taaggaccgg ataccggata ccggcgcggt
    12781 tttcttgcgg aaaaacggaa aagaaacgct gattttgccg gcgcgggaat ccaaaagcgc
    12841 gaccatttcg gcagtcagcc tatgcaattg tgcgaaatcc aagacttcgg tatgttgctc
    12901 catcaatgcg acaaagcgcg acatatgcgt ccctttctgc tcggcgggca gataaaccgt
    12961 catcgtcagg cgagcaaccg tggattgtgt tccttcggcg gttttcaggg taatcgggaa
    13021 gcgcaggtct ttaatgccga cctgattgat cggcaggttg cgcaaatctc tgctggattg
    13081 cacgtctgca atggcgttca tgcgttgttt gtccttaata ttcagataat tattgagatg
    13141 tgtgtattgt atggcaggca gatgccgtct gaaaaaacgc tgtcggccgc ctgcctgcaa
    13201 atgcgagatt atatcacttg cttttggcgg ctgcattgat tgtttctatt ttcgggatag
    13261 tgataaaatc ccgacttatc catatcttac aaggcagaaa cagatgaaat tcccgacccg
    13321 tgccatccat tccggctacg attgcgacga acacaaccgc gcgctgatgc cgccgattta
    13381 tcaaaacagt atgtttgcgc tgcacgaaat cggcgaaaat gtgccttacc gttattcgcg
    13441 cctgagcaac ccgacccgtc agattttgga agacaccgtt gccgatttgg aacacggtgc
    13501 ggcaggtttt gcgttttcca gcggtatggc ggggattgat gccgtgtggc gtaccttcct
    13561 gcgcccgggc gataccatcg tcgcggtcgc cgatatttac ggcggcgcat atgacttatt
    13621 ggtcgatgtt tatcaaaaat ggggggtgaa cgttgttttt gccgatttgg gcaatcccga
    13681 taatttggac gaattgctca aagcgcacaa tgtcaaactg gtttggctgg aaatgccgtc
    13741 caatccgctt ttacgcttgg tagacatcaa agcccttgca gcgaaagcca aagcagccga
    13801 cgcgctggtc ggcatcgaca acacctttgc cacgccgtat ctgcaacagc ccttggatat
    13861 gggttgcgat tttgcattcc attccgctac caaatatttg tgcggccatt ccgacgtatt
    13921 gatgggcatc gtcgttgcca aaaccaaaga gctggctcag cctttgcacg atatgatggt
    13981 gcataccggc gcgattgcag gcccgacgga ctgctggctg gtgttgcgcg gcatcaaaac
    14041 gcttgccctg cgtatggagg cacactgcaa aaacgcactc gaaattgcgc gccgtttgga
    14101 agcccatcct gccattgaaa aagtgttcca tcccggcctg ccgtctcacg aacattacgc
    14161 actcgcccaa gcgcaaatgc ccaaaggcat cggcggcgtg gttacggttt atctcaaaaa
    14221 cgacacgcgc gaagcggcaa acagcgtgat taaaaacatg aaactggtca aaatgacctc
    14281 cagcctcggc ggcgtggaaa gtctggtcaa ccattgctat tcccaatctc acagcggcgt
    14341 gccgcataat gtgaaaatgg aaatgggcat cagggtcgga ctcctgcgct tctccatcgg
    14401 tattgaagac gtagatgata tttggaacga tatttccgcc gcactcgata caactttgta
    14461 aaccgtaaaa aatgccgtct gaaaccatag tttcagacgg catttcaata ttgcgaatca
    14521 aatatcaacg cttcagcatc tttgccgcct caatggcgta ataggtcaaa atcccgtccg
    14581 cacccgcacg tttgaatgcc agcaggcttt ccaaaaccac tttgccgccg tccagccagc
    14641 cgttggcaac cgccgcctgc aacatcgcat attcgcccga aacctgatag gcataagtcg
    14701 gtacgccgaa ctcgtccttc acgcggcgga caacgtccaa atacggcaaa ccgggcttca
    14761 ccatcaccat atccgcacct tcctgaatat cgagcgccac ttcatgcagc gcctcatcgg
    14821 tatttgcagg atccatctga taggtctttt tatctgcctt tcccaaattg cccgaactgc
    14881 ctaccgcatc acggaaaggg ccgtagaatg cagaagcata tttggcggaa tatgccataa
    14941 tccgcgtatg gatatgtccg gcatcctcca aagcctcgcg gatggcgccg atacgcccgt
    15001 ccatcatatc ggaaggagca acgacctgcg tgcccgcctc tgcatgacat aaagcctgtt
    15061 tcaccaagac ttctacggtt tcatcattca tcacgtaacc gttttcgtcc gtcagtccgt
    15121 cctgaccgtg caccgtataa ggatcgagcg cgacatccgt cataatgccc agttcgggaa
    15181 acctctcgcg caaggctcgg acagttgacg gcacgagtcc ttcgggattg tacgcctcct
    15241 gcgcacgccc ggttttgttt gccgtaacca cgggaaagag tgccaacatc ggaataccga
    15301 gcttcaccgc ctcttccgcc gtaaacagca gcctgtccaa actctgacgc ttcacgcccg
    15361 gcatagaagg cacatcctcc tcgcgcgccg ccccctccaa tacgaacacc ggataaatca
    15421 aatcatcggc ggtcagcata tgctcgcgca tcaggcggcg tgaaaaatca tccctgcgca
    15481 tacggcgcat acgcgaagcc ggaacattgc ggtaaggaaa ttgcataaac cctccaatca
    15541 tcctgtcaac aattcaaatc atacggaagc cgccgcaact tgcagacggc atcgagcggt
    15601 aatcaaaatc cgttttcacc ggaaagggtt tcggcacgcc gaaatgccgc gccgaaggac
    15661 agcatgccgc ccattgaaag aacaatagca aaacaaccac ttcaggctgt caggcctcga
    15721 tattcttacg ccacaatacc aaaagtttcc cgatatgccg aacaggttgc gcgtcaaccg
    15781 cctcacacaa ggtattgcag atttcgatac gctcggcacg gtcgtcgccg aatacgcgca
    15841 ctttaatcag ctcatgcgcc gtcagtgccg catcggtttc cttgatgact gcatccgtca
    15901 gaccctgctg accgaccatc acaacaggat ggagatggtg cgcgcgcgct ttcagttcca
    15961 aaatttcttt ggtgttcaat ttggtatccg ccattttcct actgtttgaa taaaaaggat
    16021 aatgcagcat tgtacgcgat ttggcacggc aacggcaaaa ttaacgtaca atacaccgtt
    16081 tacattccga cccgcgaacc actatggctg tacgttccaa atcctcaaaa gcgtggctgc
    16141 acgaacacat caacgaccac tacgtccata tggcgcaaaa agacggctac cgcgcccgtg
    16201 ccgcatacaa acttttggaa atcaacgaaa aagacaagat aatcaaaccc ggcacggtac
    16261 ttgccgactt gggcagcgcg ccgggaagct ggtcgcaggt tgccgccaag ctgacgggta
    16321 cttccggaac ggttttcgcc ttggacatcc tgcctatgga agccataggg ggcgtgtcct
    16381 tcattcaggg cgacttccgc gagaacgacg ttttggcgca attcgaaacc ttgttggaca
    16441 accgcccgct cgaccttgta atctgcgata tggcgcccaa tatgtcggga aacgccgtaa
    16501 gcgatcaggc acgcagcttt tatctgtgcg aactggcttt ggacttcgcc tcgcaacacc
    16561 tgaaaaccgg cggcagcttt ttggtcaaag tctttcaggg tgcaggctat caggaataca
    16621 tggcagccat gcgcgaaatt ttcggcactg tgcagacgcg caaacccgaa gcctcgcgca
    16681 atcgctccag tgagatttat ttattgggca aaaataaacg ctgacaatac agacggcgtg
    16741 ctttacaatc atttccgttt tacacctcaa ttatggagcc ttgctaagtg gggaacacct
    16801 ttaaatcaat ccttgtctgg gtcgccttgg gtatcggcct gatggctgcg ttcaacgctt
    16861 tggacggcaa aaaagaagac aacgggcaaa tcgaatattc tcagttcatc cgacaggtca
    16921 acaacggcga agtatccggc gtcaacatcg aaggatccgt cgtcagcggc tacctgatta
    16981 agggcgagcg caccgacaaa agcaccttct ttaccaacgc gcctttggac gacaacctga
    17041 ttaaaacact gctcgacaaa aacgtccgcg taaaagtaac gccggaagaa aaaccgagcg
    17101 cgctggctgc cctgttttac agcctgctgc ccgtcctgct gctgattggc gcgtggttct
    17161 actttatgcg tatgcagacg ggcggcggcg gaaaaggcgg cgcattctca ttcggcaaaa
    17221 gccgcgcacg cctgctggac aaagatgcca acaaagtgac ctttgccgat gtcgccggct
    17281 gcgacgaagc caaagaagaa gtgcaggaaa tcgtcgatta cctcaaagca ccgaaccgct
    17341 atcaaagctt gggcgggcgc gtgccgcgcg gcatcctgct ggcgggcagc ccgggtacgg
    17401 gtaagacgct tttggcaaaa gccattgcag gcgaagccgg cgtgccgttc ttcagcattt
    17461 ccggttccga ctttgtcgaa atgttcgtcg gtgttggtgc gagccgcgtc cgcgatatgt
    17521 tcgagcaggc gaagaaaaac gccccctgca tcatctttat tgatgaaatc gacgcagtcg
    17581 gccgccaacg cggcgcaggt ttgggcggcg gcaatgatga gcgcgagcaa acattaaacc
    17641 aattattggt tgaaatggac ggttttgaga gcaatcagac tgtaattgtg attgcggcaa
    17701 ccaaccgccc cgacgtactc gatcctgcgc tgcaacgccc cggccgcttc gaccgccaag
    17761 tcgtcgtccc cctgccggac atccgggggc gcgaacagat tttgaacgtc cattctaaaa
    17821 aagtgccttt ggacgaatct gtggatttat tgtccctcgc gcgcggcacg cccggttttt
    17881 ccggcgcgga tttggcgaac ctggtcaacg aagccgccct gtttgccggc cgccgcaaca
    17941 aagtcaaagt cgatcaaagc gattttgaag acgccaaaga caaaatctat atgggtccgg
    18001 aacgccgcag tatggtgatg cacgaagacg aaaaacgtgc gacggcgtat catgagtccg
    18061 gacacgcgat tgttgccgaa agcctgccct ttaccgaccc cgtccacaaa gtaaccatta
    18121 tgccgcgcgg acgtgcgctg ggtctgactt ggcagcttcc ggagcgcgac cgcatcagta
    18181 tgtataaaga tcagatgttg agccagctct ccatcctgtt cggcggacgg attgccgaag
    18241 acatcttcgt cggacgcatc tccaccggtg catcaaacga ctttgaacgc gcaacccaaa
    18301 tggcacgcga aatggtaacg cgctacggca tgagcgacaa aatgggcgtg atggtttatg
    18361 cggaaaacga aggcgaagtc ttcttggggc gcagcgtaac ccgttcgcaa aacatttccg
    18421 agaaaaccca gcaggacatc gacgcggaaa tccgccggat tttggacgag cagtatcagg
    18481 ttgcctacaa aatcctcgat gaaaaccgcg acaagatgga aacgatgtgc aaagccctga
    18541 tggaatggga aaccatcgac cgcgatcagg tactggaaat tatggcgggc aaacaaccca
    18601 gcccgcccaa ggattacagc cacaacctgc gcgagaatgc ggacgcggcg gaagataacg
    18661 cgccgcacgc tccgactcgg gaaaaaaccg aagcacctgc cccggcagac accgcctcga
    18721 cagagtccgg gcagcagcct gaaaacaagg cttaacttcc cgaacaaacg gcagcccgca
    18781 agctgccgtt ttcccatccc gttttcagac ggcattttcc gctcaatgcc gtctgaaacg
    18841 ccaaagccgt atcggttttt gcgaatctgc gatatggcgg tttaaattga aatcagaacg
    18901 gggcaaggct gtaccggttt aaaattaaac cgctataata ccgcccttcg aatacacgcc
    18961 cgcattggtg atgcccgtga aaaaagttga aaaaaacatc ttggtgctgc acggcgcgga
    19021 caaaatgttc gagctggtcg acaaggttga ggattacccg cactttctgc cgtggtacag
    19081 caagaccgag gtcatcgggc gtagcggcaa cgaactgaag gcgcggctgt ttatggatta
    19141 tatgcgcgtt cgccaatcgt ttgccacgca caaccgcaac atccccggca gggaaatccg
    19201 tatggaactg ctcgaaggcc cgttcaaaac cttacgtgga acgtggaaat ttatcgattt
    19261 gggcgacgat atgtgcaaaa tcgaattcaa tttggaatac gatttttcca atgccgtttt
    19321 gtccgcctta atttcccccg tcttcaacca cctttccgcc acgctggtcg aagcgttcgt
    19381 caaagaggca gaccgccgtt atgcttgaaa ttgagattgt gtacggtctg cccgaccgac
    19441 aggttttgaa aaccatgcag cttgccgagg gaacaaccgt ccgcaccgcc gcactgcaaa
    19501 gcggtttgga cggcatattt gaaaatttaa acctgcattc cgcgcctttg ggcattttcg
    19561 gcaaagccgt caaagacgac acgccgctgc gcgacggcga ccgcatcgaa gtgtaccgcc
    19621 cgctgttgat cgaccccaaa gaagcgcgcc gcaaacgcgt tcaaaatcaa gaagaataac
    19681 catgccgtct gaagccttca gacggcattc gacagaaaca cggaaaatat catgtcaaac
    19741 acaatcaaaa tggttgtcgg cttgggcaac ccgggcaaag aatacgaaca gacccgccac
    19801 aatgcgggct tttggttcct cgacgaactg gcgtggaaat ggaaggcttc gtttaaagaa
    19861 gaaaaaaaat tcttcggcga agttgcccgc gccgccctgc ccgacggcga tgtttggctg
    19921 ctcaaaccgg ccacgttcat gaaccgttcc ggacaggcgg ttgccgcgct tgcacagttc
    19981 tacaaaatca aacccgaaga aatcctcgtc gtccacgacg aactcgacat cccttgcgga
    20041 cggatcaaat tcaaactcgg cggcggcaac ggcggacaca acggcttgaa agacattcag
    20101 gcaaaactcg gcacggcaga ctattaccgc ctgcgcctcg gcatcggcca ccccggcgac
    20161 cgcaacctcg tcgtcggcta cgtcttgaac aaaccgagcg cggaacaccg ccggcaaatc
    20221 gacgatgccg tcgccaaatc cctgcaggcc gtacccgaca tcatttccgg caaatgggaa
    20281 gaggcaacgc gcttcctgca cagcaaataa tccaatgccg tcttaagccc tttcagacgg
    20341 cattttcccg atttccgtat ccgaacagtc atgaccgaac tcaagcagct tatccaaacc
    20401 gaatccatcc ccgtcatcga agaaaccctc gatttcctgc tgtacgaatg cagcatcgac
    20461 gacgcaccgt ccgccgaaga agtggcacaa tggcgcgaca tacttgccgc acgcggcggc
    20521 aaattcctgc gcctgtccaa aatctgccaa acgtggctgg acgaggaggc ggcatgaagc
    20581 tgccgcgcaa ccgcttcagc ctgctttccg cattgtggtt tgccggcggc atctattcgc
    20641 tgctcttcaa agctgccgac accgcgccgc cgccgtttcc acatttcgac aaagcagcac
    20701 accttgccct gtttttcgca caaatcttgc ttctggccaa agcattcaaa accggaaaac
    20761 ttcccatccc ctaccgcagc ctgattgcgt tcgccttctg ttttgccgtc ggcagcgaat
    20821 gcgcgcaggc atggtttact gcaacgcgaa ccggcagttt gggcgatgtc cttgccgacc
    20881 tgacgggcgc agcccttgcc ctctttgccg cgcgttctgc ctgccgcccg gactaaatcg
    20941 gtttatttct cccaaacagg atgaatcccc ctgatgcatc ctgtttttcc ccttacatat
    21001 caatcccatc tcataaacaa aaccgacaaa tcgtttacaa tatatttaca ctacaccgaa
    21061 ttacaaatat actcgaagcg gttgcagaac cgacggtttc gcaaaccgtt tcggattgca
    21121 cgacacaaaa gaaaaaccga ttttgttgcc ttaaaggagc attcatgaac ctgcatgcaa
    21181 aggacaaaac ccagcatccc gaaaacgtcg agctgctcag tgcgcagaag ccgattaccg
    21241 actttaaggg tttgctgacc accattattt ccgccgtcgt ctgtttcggc atttaccaca
    21301 tcctgcctta cagccccgat gccaataaag gtatcgcgct gctgattttc gttgccgcac
    21361 tttggtttac cgaagccgtc cacattacca taaccgcact gatggtgccg attctcgccg
    21421 tcgtactcgg tttccccgac atggacatca aaaaggcgat ggctgatttt tccaacccga
    21481 ttatctacat ttttttcggc ggcttcgcgc ttgccaccgc cctgcatatg cagcggctgg
    21541 accgtaaaat cgccgtcagc ctgttgcgcc tgtcgcgcgg caatatgaaa gtggcggttt
    21601 tgatgttgtt cctcgttact gcctttctgt ccatgtggat cagcaacacc gccaccgccg
    21661 cgatgatgct gcctctagca atgggtatgc tgagccacct cgaccgggaa aaagaacaca
    21721 aaacctacgt cttcctcctg ctcggcatcg cctattgcgc cagcatcggc ggcttgggca
    21781 cgctcgtcgg ctcgccgccc aacctgattg ccgccaaagc cctaaatctg gacttcgtcg
    21841 gctggatgaa gctcggcctg ccgatgatgc tgttgattct gcccttgatg ctgctctccc
    21901 tgtacgtcat cctcaaacct aatttgaacg aacgcgtgga aatcaaagcc gaatccatcc
    21961 cttggacgct gcaccgcgtc atcgcgctgt tgattttcct tgccacagcc gccgcgtgga
    22021 tattcggctc caaaatcaaa accgccttcg gcatttccaa tcccgacacc gttatcgccc
    22081 tgagtgccgc cgtcgccgtc gtcgtcttcg gcgtggcgca atggaaggaa gtcgcccgca
    22141 ataccgactg gggcgtgttg atgctcttcg gcggcggcat cagcctgagc acgctgttga
    22201 aaacatccgg cgcgtccgaa gccttgggac agcaggttgc cgccaccttt tccggcgcgc
    22261 ccgcattttt ggtgatactc atcgtcgccg ccttcattat ttttctgacc gagttcacca
    22321 gcaacaccgc ctccgccgcc ctgcttgtac cgattttctc cggcatcgct atgcagatgg
    22381 ggctgcccga acaagtcttg gtattcgtca tcggcatcgc cgcatcttgc gccttcatgc
    22441 tgccggttgc cacgccgcct aacgcgattg tgttcggcac gggcttaatc aaacaacgag
    22501 aaatgatgaa tgtcggcata ctgctgaaca tcctatgcgt agtattggtt gctctgtggg
    22561 cttatgctgt actgatgtaa accatcgacc taaacaacaa gaccgtctga aagaatattt
    22621 ttcagacgac cttgaagttt gtcgttcaga cacaatttgt cgaataattc aaaaccggat
    22681 tctaacgaaa ggaaaaccca tgattatcct gcacaccaac aaaggcgaca tcaaaatcga
    22741 actcgatttc gacaaagccc ctgttaccgc caaaaacttc gagcaatacg tcaaagacgg
    22801 cttctacgac ggcgtaatct tccaccgcgt catcaaaggc ttcatgattc aaggcggcgg
    22861 catggatgaa aacatgaacg aaaaagaaac ccgcgatccg attcaaaacg aagcgtccaa
    22921 cggcctgccc aacgataaat acaccatcgc catggcgcgc acttccgacc cccattccgc
    22981 cggcgcgcaa ttcttcatca acactgccga caacgctttc ctaaacttcc gttctaaaga
    23041 gctgtacggc aaaaccgtcg tccaagactg gggctatgcc gtattcggta aagtcgttga
    23101 cggttttgac gttgtcgatg ccatcgaaag cgtatccacc aaacgccacg gctaccacga
    23161 cgatgtgccg accgaacctg tcatcatcat taaagccgaa gcagtataaa ccggcaatcc
    23221 gaaagcagcc tgcataaaag gctgctttta tttcagacgg catgttattt gatgtaaccg
    23281 ggctatgccg tctgaaaacc ggacgcaagc cttcagacgg cataccgcta tgttaaaata
    23341 cgcccgtttt tcctaaacgg acacaaagga aaccttatgg caagcatcac ccgcgacatc
    23401 ttcaaagcct acgacatccg tggcatcgtc ggcaaaaccc tgaccgacga tgccgcttat
    23461 ttcatcggca gggccatcgc cgccaaagcc gccgaaaaag gtatcgcccg catcgcgatc
    23521 ggacgcgacg gacgcttgag cggccccgaa ctgatggagc acatccaacg cggcctgacc
    23581 gacagcggta tcggcgtact caatgtcggc atggttacca ctcctatgct ctacttcgca
    23641 gccgtcaacg aatgcggcgg cagcggagtg atgattaccg gcagccacaa tccgcccgat
    23701 tacaacggtt tcaaaatgat gctcggcggc gacacgctcg caggcgaagc cattcaagaa
    23761 cttttagcta ttgttgagaa agacggtttt gttgccgccg acaaacaagg cagcgtaacc
    23821 gaaaaagaca tctccggcgc ataccacgac cacatcgtcg gacacgtcaa actcaaacgc
    23881 ccgataaaca tcgccatcga cgcgggcaac ggcgtgggcg gcgcgtttgc cggcaaactc
    23941 tacaaaggtt tgggcaacga agtgaccgaa cttttctgcg aagtggacgg caatttccct
    24001 aatcaccacc ctgatccttc caaaccggaa aacctgcaag atttgattgc cgcgctgaaa
    24061 aacggcgatg ccgaaatcgg cttggcgttt gacggcgatg ccgaccgctt gggcgtggtt
    24121 accaaagacg gcaacattat ttatcccgac cgccaactga tgctgttcgc ccaagacgtt
    24181 ttgaaccgca atcccggcgc gaaagtcatt ttcgatgtca aatccacacg cctgcttgcc
    24241 ccgtggatta aagaacacgg cggagaagcc ataatggaaa aaaccggcca cagcttcatc
    24301 aaatccgcta tgaaaaaaac cggtgcactg gttgccggcg aaatgagcgg acacgttttc
    24361 tttaaagaac gctggttcgg cttcgacgac ggcctgtatg ccggcgcacg cctcttggaa
    24421 atcctgtccg cctccgacaa tccgtccgaa gtgttggaca acctgccgca aagcatttcc
    24481 acgcccgaac tcaacatctc cctgcccgaa ggcagcaacg ggcatcaagt tatcgaagaa
    24541 ctcgccgcca aagccgaatt tgaaggcgca accgaaatca tcaccatcga cggcctgcgc
    24601 gttgaatttc ccgacggctt cggtctgatg cgtgcttcca ataccacgcc gattttggtg
    24661 ttgcgttttg aagcggatac gcaagcagcc atcgagcgca ttcaaaaccg attcaaagcc
    24721 gtcatcgaaa gcaatccgca tttaatctgg cctctgtaaa aatagaaaaa atgccgtctg
    24781 aaactttcag acggcatttt tcacaagtag ccaatcaaat cttggcagat tggatttggc
    24841 ttaaaaatct ccgcgtccgt tcgtgtttga ggtggtcgaa caactctttc gggctgccct
    24901 gctctacgat aacgccgccg tccatcacga cgacgttcgt ggcaacttcc agcgtgaact
    24961 tgatttcgtg ggtaacgacg accatcgtcc aaccttcccg cgccaattcc ttcatggcgt
    25021 ccaacacgtc ttgcaccaac tcggggtcca gcgcggaagt gggttcgtca aacagcatca
    25081 attcaggctg aatcgccagt gcgcgggcga taccgacacg ctgctgctga ccgccggaaa
    25141 gctggtaggg atacaaatcc actttatcgc ccaagccgac tttttccagc agtttcaaag
    25201 cctcttcgcg cgcttgggcg gcaggcttgc cctgtacggc aaccggccct tccatcacgt
    25261 tttccaacac ggttttatgc gggaagaggt tgtattgttg gaataccatt ccggacttgc
    25321 ggcgcagtgc caaaatatcg tgtttgcttg tttttttgga aaaatcaatg cgtaacggcc
    25381 gcgcgttgtc gaactcgatt tgtccgtctt cgggcatttc caacgcgttt aggcagcgca
    25441 gaaatgttgt tttacccgag ccggaaggcc cgaggatgac gaccacctgc cctttgccca
    25501 catccaaatc gatgccgcgc aaaatggtgt tttcgccaaa ggtcttatgg atattgcgga
    25561 ttttaatcat gacaactcct tatttggcga cataacggtc gaaacgtttt tccaaacgcg
    25621 cctgaatcaa aaacagcact ttacagaaac accaataaac caatgcagct tcgatataga
    25681 caggcaaaaa gtcataagtg cggtttgccg tttcctgtgc gacacggaaa agctccgtta
    25741 ccgttaccac ggcggcaagc gaggtgtttt tgaacaagcc gataaactcg ttgctcaacg
    25801 gcggaacggc gacgcggaat gcctgcggtg cgacgatgcg gcggaacgtc tgcatatagg
    25861 tcataccgat ggagaaacct gcttcccact gccctttcgg cacggacaaa atcgccgcgc
    25921 gtatggtttc ggaagcgtat gcgccgacat tgagcgaaaa gccgatgatg gcggcgggaa
    25981 tcggattgat atagatgccg acggacggca gcccgtaaaa cacaatcaca agctgaacca
    26041 acagcggcgt accgcgaacg acggaaatat aaaattccac cagcttcaac aagcattttt
    26101 ggaaaatacc gccggaaggc atgattctta ccaaagcaac ggctacggca ataatcatgc
    26161 cgataacgaa agaagcgatc gccaaaggca aagacactgt aaagccggct ttaaccatgg
    26221 gccaaaacgc gctgatgagc atatcagcgc gtgtttccgt cataaacggc agagaggcaa
    26281 ggaaattatt gaacactgat gtcttttccg aagaattgtt cgcccagttt tttcaacgtg
    26341 ccgtcggctt tcagctcgtt gattgccgtg ctgaatttcg ccacggcctc gtcattgccc
    26401 ttgttgacaa tcagaccgga accgactttt tcatcggcag gcgcggacca cacgattttc
    26461 acccccgcat tcgggttttt cttcagatag tccaaaaccg ccaattcatc gttcaacgtc
    26521 gcatcggcgc gtttttgttc aatcagggtc agcgattgcg ccaaaccgtc caccggcacg
    26581 agttgcgcac ctgcggcttt ggctttttcg ccgtagttgc tggtcaggga ttgcgcggtt
    26641 ttcacgcctt tgatgtcggc tatggattta atgttgctgt cgttacgcgc aaccaaaacc
    26701 gcaccgctcc agctgtaagg ttcggatttg tcaaatgtcg cctggcgttc ggggctggtc
    26761 aggccgactt ggtttgccac cacgtcgaaa cgtccggctt tcaaacccgc catcatcgaa
    26821 tcccattgcg tttctttaaa ctcgactttt acgcccagtt tttccgccac ggcgcgggtt
    26881 acttccacgt cgtaaccggt cagtttgccg tctttgtcgt ggtaggtaaa cggtgcgtaa
    26941 gtgccttccg tgccgacggt aaccgtgcct ttattgttga tgcgctcgat taaagaaccg
    27001 gaaattgccg attgtgcagg cgcggaagat gctccgctgc cgccttccga accgccgcag
    27061 gccgccaaaa ccaatgcggc aatgccgccg agtacgaatt ttttcaacat cattccctcc
    27121 tgtaaaaagt gtgcaagtgc ggcattctaa gtgaaattgc ggcaaacaca aagaatactt
    27181 tttttatatg gttattccaa atcggaatgg gaaagatatg ccgaagccgc cggaagcgga
    27241 aataaagggg gcgggaaagg cggcgggcgg cacgaaaccg aaaagcggca tcaggtatgc
    27301 aaacggcgga ttcacgcacg ccgttccggc aaaaatccgc ctgccgattc ggtgctataa
    27361 tccacccgaa acagatgaca ccaaaaagga acatctcatg aacacgcccc aatccgccat
    27421 cattcccgac cacgcccaag ccggcatctt tatcgaagcc gacttcgccg ccaaccgcct
    27481 caacgatatt aaagccgcct gccgcgcttc gctcgacgcg ttatccgcct tgaaagcccg
    27541 ctttccggac gatattttgg gtctgaccat cgccttcggc agcaaagctt gggcaacatt
    27601 cggacacacg gacgaaggca gtgaaatcaa acccttcccc gaaatgggca acgggcttgc
    27661 gccgtccacg cagcacgata tgtccatcca catccaatcc ttccgccaaa acgccgccta
    27721 cgcgcttgcc caatccgttt tgggcgcgtt cggcgacagc atatgcgtcg cgtccgaaga
    27781 acacggtttg cgcctgtatc aggatcgcgg gctggacggt ttcgtcgacg gcacggaaaa
    27841 cccgcaaggc gatgaaacaa tccgcgaagt cgccatcatc cccgaagggc tgcccgatgc
    27901 aggcggcagc tatgtcctgc tgcaaaaata cctgcacgat ttgaaaaaat gggatgccgt
    27961 ccccgtcgcc gaacaggagg cctcggtcgg acgcagcaag gaaaccgacg acgaattcag
    28021 ccgcgatgtc cgcctgcccg attcgcacct cggccgcgtc aacctgaaag aaaacggagt
    28081 cggcctgaaa atcgtccgcc gcagcctgcc cttcggcaaa atcagcggcg aacacggttt
    28141 gatgtttacc gcatactgcc gcacgctgca taatatcgaa gcgcaattat tgagtatgtt
    28201 cggcgacacg gacggcaaaa ccgacctgct gctccgacac ctctccgccg ccgtttcggg
    28261 cggatactac tacgcgccgt ctgtcgaacg cctgcaaaac ctctaagccg gacgatgccg
    28321 tctgaaaccc tttttcagac ggcattttcc cgttttaccg tccaaccgtt ttcatacacg
    28381 ctatgtccgc acccatccga gcattcgacc cgctgaccgc ccccatttcc ggcaccaacc
    28441 tgattgaggc ctctgccggc accggcaaaa cctacggcat tgccgccctg tttacgcgcc
    28501 tgatcgtatt ggaacaaaaa agcgtcgagc gcgtattggt cgttaccttc accaaagccg
    28561 ccactgccga gctgaaaaca cgcctgcgcg cgcgtttgga cgatgtgttg caggttttgg
    28621 aaagcaaaga aattgccgaa cttggagacg acacgctttc agacggcatt gccgcctact
    28681 gcgccgaaca ccacgaaggc gacaccttcc tgcccgcact cttggaacag gctttgcaaa
    28741 aagaaagccg gacgcgtctg attgtccgcc tcaaggccgc catcgggcaa ttcgacaacg
    28801 ccgccatcta caccatccac ggcttctgcc agcgcatcct gcgcgactac gccttcctgt
    28861 gccaagcacc gttcgatgtc gaactgaccg aagaagacgg cgaccgcctg cttgtcccgg
    28921 ctcaagattt ttggcgggaa cgcgtcagcg gcgacccggt gcttgccgca ttggcgttta
    28981 aacgcaaagc tgtgccgcaa accgtccttg cccaaatccg cgcctacctg tcccgcccgt
    29041 acctgaattt ccgccgtccg caggcggatt tgaaacaggc gcagcgcgac gccgaaacct
    29101 catggcaaac cgtctgccgc ctgctgcccg agctggaagc cggcttttgg cgcatccatc
    29161 ccgacctcaa cggcaacagt taccgcaaaa acagcttcgg caaccttttt aaagaactgg
    29221 cacaaaaatc cgccgccgga caactgccct ttctggacaa agacacgcac gaacggctct
    29281 tgaaactctc atccgacaaa ctcgaagccg gactgaaaaa aggcaaaacg cccgatgcgg
    29341 cagtatttgc cgaattgcag aaactggcag acttcgggcg cgatttgaac gcactcgaag
    29401 aagcggaaga agcaacaatg atccggctgc aactggattt aatcgaatat ctcaacagca
    29461 gccttgccga aatgaaaaaa tcgcgccgcg aacgcggttt cgacgacctg ctgctcgatg
    29521 tccacaccgc gctgaccgac aatccgcacg ccgaaacccc cgcccgcgcc gttgccgaaa
    29581 actgggaaat cgcgctgatc gacgagttcc aagacaccga cccgctgcaa tacgaaatct
    29641 tccaaaagat tttcatcgcc cgaaaccgcc cgctgttcct cgtcggcgac cccaaacagg
    29701 cgatttacag ctttcgcggt gcggacattt acgcctacct tcaggcggcg gaagacgcgc
    29761 ggcaccgcta cacgctcgcc accaactacc gcagccacgc cgcgcttatc ggcagcatcg
    29821 gcgcgctgtt ccgcctcaaa gaacgcccgt tcgttttgga aaacatcggc tattcggaag
    29881 tcggtgcggc gcgtgccgaa agcaggttgt cgcccaaacg tcctgccgta cagctccgtt
    29941 ggctgcacga aaacgacaat gaaaaagcca acaaagacgt tttgcgccgc cgtgccgccg
    30001 actattgcgc cgacgaaatc gcccacgcgc tcaacgaagc ctccggaggc cgtctgaatt
    30061 tcaaaggccg cccgttgcag tcgggcgata ttgccgttct ggtacgcacg cacaacgagg
    30121 cggtgatgat ttccgccgca ctgaaaaaac ggcaggtgca aagcgtcctg ctttcgcgcg
    30181 aatccgtgtt cgcctcgccc gaagccgccg ccctgtccgc gctcatcggc ttctggctcg
    30241 aaccgcgccg cgccggaacg ctgcgctttg tcctgacaag cggcatattc ggttatgacg
    30301 cgcagcaatt gcacgacttc aaccaaaacg aaagcgagat tttgcattgg gcggaatctg
    30361 cccgaaccgc gctcgacata tggcaaaaat acggcatttt cgccgctatg cagcagtttt
    30421 cccaaacaca cggcatcgaa acgcgcctct taagccggaa caacgggcgc agcctgacca
    30481 attatttcca actgctcgaa ctgcttgccg ccgaagacgc gcaaaaccgc aaccccgccg
    30541 cactgcacaa atggctgcgc gatcaaatca gcctggccgg caacaacggc ggcgacaacc
    30601 gcgccatccg tttggaaagc gacgaagatt tggtcaaaat cgttaccatg cacgcctcga
    30661 aaggtttgca gtatccgctg gtgtactgcc cgtttgcgtg ggacgcgcaa gataccggac
    30721 cgtccgactg gcaaatcctc caccaaggcg caaaccgggc cgaacttttg gcaaaggcgc
    30781 aactgtcgga aagcgaacag aagcaatacg ccgacgaaga aatggcggaa cgcctgcgcc
    30841 tgctttatgt cgcgctgacg cgtgccgagg aacagctcaa catctacgcc gcctattctt
    30901 ccgataccgc cgacaacccc cttgcctacc tgatcgaagg cttgccgcaa gacagccgcg
    30961 aaaccgtccg ccgtacctat gcgtgtgaaa aagacggcat aacgatgctc aaacgcaact
    31021 ggcggcgcgt ggcggacaat gccccggccg gcacggattt cgccttcaca gaagatgcgc
    31081 cgccccctgc cgcctatcgc ggcaacgccg atcaagccgc cgaatttgcc gcaaacagca
    31141 ttcccgaacg cggattccga tttgtccgcc acaccagctt caccgcctta agccgccaaa
    31201 cccaaacgcc cgacggcggc gaagaagatg cctgcccgtc cttggatgcc gccgaaacct
    31261 cggtgccggc gatgccgtct gaaacgccga cggcttcaga cggcatatcg atacacgact
    31321 ttccgaaagg cacgcaggcg gggctgtgcc tgcacgaaat tcttgaagat ttcaaattcg
    31381 gacaagcagc cgccgaacag gaaaccctca ttgccgacaa gctgaaaaaa tacggttttg
    31441 aagaaatatg gctgcccgcc gttgccgaaa tggcggaagc ctgccgcaaa acgccgctga
    31501 ctggggcata cggcctgtcc gacatcccgc ccgggtgccg ccgtcccgaa atgggcttta
    31561 ccctccacac cgaagacttc ggcctcaaac ggctgcgcga ctggcttgcc cgcgacgaca
    31621 tcaggctgcc cgaagtctgc cgtgccgccg ccgagacgct cgacttccac accgtcaacg
    31681 gctttttaaa cggctttatc gatatggtct gccaagaccc cgacggcaat atctgcatca
    31741 tcgactacaa atccaaccac ctatccgcct acacgcggca ggcgatggac gaagccgtcg
    31801 cgcaccagca ctattacctt caggcactga tttacgccgt tgccgccgcg cgctacttca
    31861 aactgcgcgg acaaccgccc gccgccgttt ccgtccgcta cctgtttttg cgcggattgg
    31921 atggcaaagg cggcggcgtt tggcgttggg acatagatgc cgccgctttg gaacagataa
    31981 aataacttcc cgtcccttaa gacgacaacc gcaggagaac cctatgaata tcaaacaatt
    32041 gattaccgcc gcactcattg cctcagccgc ctttgccacg caggcagccc cgcaaaaacc
    32101 cgtatccgcc gcccaaaccg cgcaacattc agccgtttgg atcgatgtcc gttccgaaca
    32161 ggaatttagc gaaggtcatt tgcacaacgc ggtcaacatc cccgtcgacc aaatcgtccg
    32221 ccgcatatac gaagccgcgc ccgacaaaga cacgccggtc aacctctact gccgcagcgg
    32281 acggcgtgcc gaagccgccc ttcaagagct gaaaaaagca ggttatacaa atgttgccaa
    32341 tcacggcggt tatgaagacc tgctcaaaaa agggatgaaa tgacgcaatg ccgtctgaaa
    32401 ggacaggctt tcagacggca tacggcgttc cggcacgcgg cacggcatca ggcgcggcgc
    32461 gtgccgcccc gtccgaagcc cgataaacaa accgataagg aaatacgatg aaacacatac
    32521 tccccctgat cgccgcatcc gcactctgca tttcaaccgc ttcggcacac cctgccgaca
    32581 aaccgcccac ccaaaacgaa accgctatga ccacgcacac cctcacctcg aaatacagtt
    32641 ttgacgaaac cgtcagccgc cttgaaaccg ccataaaaag caaagggatg gacatttttg
    32701 ccgtcatcga ccatcaggaa gcggcacgcc gaaacggcct gaccatgcag ccggcaaaag
    32761 tcatcgtctt cggcacgccc aaggccggta cgccgctgat ggtcaaagac cccgccttcg
    32821 ccctgcaact gcccctgcgc gtcctcgtta ccgaaacgga cggcaaagta cgcaccgcct
    32881 ataccgatac gcgcgccctc atcgtcggca gccgcatcag tttcgacgaa gtggcaaaca
    32941 ctttggcaaa cgccgaaaaa ctgatacaaa aaaccgtagg cgaataagcc cgtcgccgac
    33001 atatggcgga tggaaatcaa atgcacacgc ccctcattcc cccgaagtcg ggaatccaag
    33061 cggttgggtt accgtgattt ccaatcattg ccgaaacgga cgattccgga ttcccgcctt
    33121 cgcagggatg acgggcgtat gcgttttgcc atatcacagg caactttgcc gcaaatctta
    33181 tctgtcgccg gtatgccttc ttgtttcaat ccgccacaaa ccaaatgccg tctgaaacct
    33241 gatttcagac ggcatttccg tttattccct gctgtcgcgg caaatatcga tggcttcctg
    33301 caaacttgaa acgccgtgga ttttcaggtt cggaaactct ttggcattgc gcggcatatt
    33361 ggctttgggg acgatggcgc gtttgaagcc gagcttttcc gcttctttga gccgctcttg
    33421 tccgcgtgcg acggggcgga cttcgccgct taggccgatt tcaccgaaga ccacggtttt
    33481 ttcaggcata gggcggttgc ggaagctgga aagcatcgcg aggatgaccg ccaaatccgc
    33541 cgccggttcg ccgattttca cgccgccgac ggcgtttaaa aaaacatcct gatcgaaaca
    33601 ggcgatgccg ccgtgccggt ttaacacggc aagcagcatc gcaaggcggt tttgttccaa
    33661 tccgacgctg aggcgtttgg gtgtgaagcc gtgcgcgtca tcgaccaatg cctgaatttc
    33721 gaccaaaagc gggcggctgc cttcctgtgt aaccaaaacg cacgaaccgg gcgtgtcgtc
    33781 gcggtagctg gcgaggaaga tggcggacgg gttggatacg cctttcaaac cgttttcggt
    33841 catcgcgaac acgcccaatt cgtttgccgc gccgaaacgg tttttgatgg cgcgtatcat
    33901 gcggtagttg gaatgttggt cgccctcgaa atacagcacg gtatcgacca tatgttccag
    33961 cacgcgcggg ccggcaatcg ctccgtcttt ggtaacgtgt ccgaccagta tcatggcgat
    34021 acccatttgt ttcgccatac gcgtcagttg ggcggcacat tcgcgcacct gcgacacgga
    34081 accgggggcg gacgtgattt ggtcggaata catggtttgg atggaatcga taacgacgac
    34141 ttcgggctga tgctgtttca aggccgcctg aatcgcttcc atgcggattt cggcaagcag
    34201 gtttacgcct tcggcgtgca gttccaaacg ttgcgagcgc agggcgacct gttgggcgga
    34261 ttcttcgccg gaaacgtaca gcactttgcg gctttgcgcc attttggcga tggtttgcaa
    34321 caacagcgtg gatttgccga tgccgggatc gccgccgagc aggatgaccg cgccatcgac
    34381 caaaccgccg cccaatacgc ggtcgagttc gcccataccg gtcggattgc gcggcacttc
    34441 ggcagcggta acggcggaga gggattggac ggtcgaagcg tccgccgccc aagattggaa
    34501 tcgggcgttt ttcggctcgg gcgcggcaaa gctttcctga agcgtgttcc actcgccgca
    34561 atgcggacat ttgccctgcc atttcgggga agtgccgccg cattcggtgc attggtaaag
    34621 ggttttaagc gtttttgcca tgatgttttc cctatgggat ttttgaagtg taaaaaaatc
    34681 gtctgaaact tcttttctaa aaaatttttt attattagtt tgttaatttt gattacaaga
    34741 ccatatcacg atacaaacaa gggtggcttt ctgttatttt gcggcataat gacggaaaat
    34801 acaaaacagg aatcccaaac aatgactttc ccattaaata taatggtaga
//
LOCUS       DAXDXW010000015        34458 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXDXW010000015 DAXDXW010000000
VERSION     DAXDXW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34458
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.015"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(184..1074)
                     /gene="sucD"
                     /locus_tag="ACFB64_000610"
     CDS             complement(184..1074)
                     /gene="sucD"
                     /locus_tag="ACFB64_000610"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC1209026.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLDLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(1085..2305)
                     /gene="sucC"
                     /locus_tag="ACFB64_000611"
     CDS             complement(1085..2305)
                     /gene="sucC"
                     /locus_tag="ACFB64_000611"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC1209027.1"
                     /translation="MGLAAGRFLKPTHLKEKSMNLHEYQAKELLASYGLPVQGGILAH
                     NGEEAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVKSREKAKEVAESLIGTNLVTY
                     QTDANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETP
                     EKIFKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFAKLMTGAYKAFVENDFALFEVN
                     PLAVRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEG
                     NIGCMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVL
                     INIFGGIVRCDMIAEAIVAAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADG
                     LNDAAEKIVAAVNA"
     gene            complement(2323..2610)
                     /locus_tag="ACFB64_000612"
     CDS             complement(2323..2610)
                     /locus_tag="ACFB64_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209028.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAVMYLLARMWLPFSK"
     gene            complement(2730..4163)
                     /gene="lpdA"
                     /locus_tag="ACFB64_000613"
     CDS             complement(2730..4163)
                     /gene="lpdA"
                     /locus_tag="ACFB64_000613"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC1209029.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            complement(4424..5605)
                     /gene="odhB"
                     /locus_tag="ACFB64_000614"
     CDS             complement(4424..5605)
                     /gene="odhB"
                     /locus_tag="ACFB64_000614"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC1209030.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATVAAEAPAAAPAEAAPAAVPAA
                     AQNNAAMPAAAKLAAETGVDVNVLQGSGRDGRVLKEDVQNAAAKPAAAVAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVTALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPVRLLLD
                     L"
     gene            complement(5705..8533)
                     /locus_tag="ACFB64_000615"
     CDS             complement(5705..8533)
                     /locus_tag="ACFB64_000615"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC1209031.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALRFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQVEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREKIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLLTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRNMGEGLGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(8738..10021)
                     /gene="gltA"
                     /locus_tag="ACFB64_000616"
     CDS             complement(8738..10021)
                     /gene="gltA"
                     /locus_tag="ACFB64_000616"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC1209032.1"
                     /translation="MSKSIKLNVPGRAGLELPVLEASIGHDVVDIRGLTKNTGLFAFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDITNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPAGER"
     gene            complement(10133..10381)
                     /locus_tag="ACFB64_000617"
     CDS             complement(10133..10381)
                     /locus_tag="ACFB64_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC1209033.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALIHGHSETDKGHLIPMLEKIRRA"
     gene            complement(10385..11092)
                     /locus_tag="ACFB64_000618"
     CDS             complement(10385..11092)
                     /locus_tag="ACFB64_000618"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC1209034.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRSLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRVV"
     gene            complement(11212..12975)
                     /gene="sdhA"
                     /locus_tag="ACFB64_000619"
     CDS             complement(11212..12975)
                     /gene="sdhA"
                     /locus_tag="ACFB64_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC1209035.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVQEIMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(12978..13319)
                     /gene="sdhD"
                     /locus_tag="ACFB64_000620"
     CDS             complement(12978..13319)
                     /gene="sdhD"
                     /locus_tag="ACFB64_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC1209036.1"
                     /translation="MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLVVLFALPKEY
                     PAWQAFFSQAWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSVKVIWG"
     gene            complement(13313..13690)
                     /gene="sdhC"
                     /locus_tag="ACFB64_000621"
     CDS             complement(13313..13690)
                     /gene="sdhC"
                     /locus_tag="ACFB64_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC1209037.1"
                     /translation="MSVKLRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGILWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(13977..15380)
                     /locus_tag="ACFB64_000622"
     CDS             complement(13977..15380)
                     /locus_tag="ACFB64_000622"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC1209038.1"
                     /translation="MKKIQADIVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMRRVKSERDRFVGFVV
                     TDVEEWPADKRIMGSAKFIDEHTVQIDDHIQIAAKSFVIATGSRPVILPQWQSLGDRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIDKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGSGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQTDEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(15629..16366)
                     /locus_tag="ACFB64_000623"
     CDS             complement(15629..16366)
                     /locus_tag="ACFB64_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC1209039.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAICCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKVKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(16645..18921)
                     /gene="metE"
                     /locus_tag="ACFB64_000624"
     CDS             complement(16645..18921)
                     /gene="metE"
                     /locus_tag="ACFB64_000624"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC1209040.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLDVAKDLRE
                     KNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLPLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQTLGLKAKPTVVGPL
                     TFLWVGKEKGSVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLNKVSAKILLGTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFAGY
                     DKVLSAGVIDGRNIWRANLNKVLETVGPLQAKLGERLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPVNADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSASDYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQNLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLAAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(19057..19935)
                     /gene="metF"
                     /locus_tag="ACFB64_000625"
     CDS             complement(19057..19935)
                     /gene="metF"
                     /locus_tag="ACFB64_000625"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC1209041.1"
                     /translation="MNYAKEINALNNSLSDLKGNINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASHDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPKWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGARP"
     gene            20132..20407
                     /locus_tag="ACFB64_000626"
     CDS             20132..20407
                     /locus_tag="ACFB64_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC1209042.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAGTHGKTMVWTDGKE
                     YPLFSLDTSSSSHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            20407..20532
                     /gene="ykgO"
                     /locus_tag="ACFB64_000627"
     CDS             20407..20532
                     /gene="ykgO"
                     /locus_tag="ACFB64_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC1209043.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKSRQR"
     gene            complement(20618..20836)
                     /locus_tag="ACFB64_000628"
     CDS             complement(20618..20836)
                     /locus_tag="ACFB64_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209044.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRVLREVAEISAWVLLG
                     AAAATLFWYLFMLYFKHIPDSYRRKNAV"
     gene            20979..22118
                     /locus_tag="ACFB64_000629"
     CDS             20979..22118
                     /locus_tag="ACFB64_000629"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC1209045.1"
                     /translation="MSQNTSVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPMVTVKDWVKSQAALADYLGIEQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYSVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVTAYMNNVDKDCRL"
     gene            22115..22696
                     /gene="metW"
                     /locus_tag="ACFB64_000630"
     CDS             22115..22696
                     /gene="metW"
                     /locus_tag="ACFB64_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC1209046.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALAEHKKCSGYG
                     IEIDTDNVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RLAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            22813..23610
                     /locus_tag="ACFB64_000631"
                     /pseudo
     CDS             22813..23610
                     /locus_tag="ACFB64_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
     gene            complement(23790..24350)
                     /gene="efp"
                     /locus_tag="ACFB64_000632"
     CDS             complement(23790..24350)
                     /gene="efp"
                     /locus_tag="ACFB64_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC1209047.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVDYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(24393..25544)
                     /gene="earP"
                     /locus_tag="ACFB64_000633"
     CDS             complement(24393..25544)
                     /gene="earP"
                     /locus_tag="ACFB64_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC1209048.1"
                     /translation="MNTYAFPACWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWT
                     DDVSALRALCPDLPDVPFVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLN
                     IIRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYRE
                     AVRFDTEALRRRLVLPEKNAPEWLLFGYRGDVWAKWLDMWQQAGSLMTLLLAGAQIID
                     SLKQSGVIPQNALQNDGGVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVR
                     TQLAGKPFFWHIYPQDENVHLDKLHAFWDKAYGFYTPETASVHRLLSDDLNGGEALSA
                     TQRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(25563..25841)
                     /locus_tag="ACFB64_000634"
     CDS             complement(25563..25841)
                     /locus_tag="ACFB64_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209049.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFETIKHHLKQGFDLKRQTMFLFIPIVLLVVYLFHYFGAF"
     gene            26192..27133
                     /gene="miaA"
                     /locus_tag="ACFB64_000635"
     CDS             26192..27133
                     /gene="miaA"
                     /locus_tag="ACFB64_000635"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC1209050.1"
                     /translation="MPTPKAFTLLGPTACGKTALALKIAETLPVEIISLDSALLYTGM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLTGEITARGKCPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGRPMSAHLNGQPEHTLPFELYTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEHLDGATDRQTFIEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSGTRLIEAAKRFFGE"
     gene            27137..27856
                     /gene="tadA"
                     /locus_tag="ACFB64_000636"
     CDS             27137..27856
                     /gene="tadA"
                     /locus_tag="ACFB64_000636"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC1209051.1"
                     /translation="MLATPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTPPQEMSQAHKDCLLAELKNHPPVAAFPPQEEMGYFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIITSAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            27861..28349
                     /locus_tag="ACFB64_000637"
     CDS             27861..28349
                     /locus_tag="ACFB64_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC1209052.1"
                     /translation="MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGILWTVGLISLGGILADYMAGMLGVKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILLILLVKYIAY
                     LF"
     gene            28415..29167
                     /gene="rlmB"
                     /locus_tag="ACFB64_000638"
     CDS             28415..29167
                     /gene="rlmB"
                     /locus_tag="ACFB64_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC1209053.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSITELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDIGGNADLYHCNLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNKKA"
     gene            complement(29229..30603)
                     /locus_tag="ACFB64_000639"
                     /pseudo
     CDS             complement(29229..30603)
                     /locus_tag="ACFB64_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
     gene            30983..31858
                     /gene="dapA"
                     /locus_tag="ACFB64_000640"
     CDS             30983..31858
                     /gene="dapA"
                     /locus_tag="ACFB64_000640"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC1209054.1"
                     /translation="MLQGSLVALITPMNQDGSIHYDQLRQLIDWHIENGTDGIVAAGT
                     TGESATLSVEEHLSVIEETVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAVPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNVGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCGPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            31867..33063
                     /gene="bamC"
                     /locus_tag="ACFB64_000641"
     CDS             31867..33063
                     /gene="bamC"
                     /locus_tag="ACFB64_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC1209055.1"
                     /translation="MPSEPFGRHNMTNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIESEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGDKNKDTTMWQPSASDPNLEAAFLTRFMQYLGVDGRQAENALAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTGLALDRIGLTVVGQNTERRAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVADGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(33120..34052)
                     /gene="pip"
                     /locus_tag="ACFB64_000642"
     CDS             complement(33120..34052)
                     /gene="pip"
                     /locus_tag="ACFB64_000642"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC1209056.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFHPDVFRIVIIDQRGCGRSHPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEGVDEDAYASL
                     AIARLENHYFVNGGWLQGDKAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWELSKAFP
                     EAELRVVQAGHCAFDPPLADALVQAVEDILPRLL"
BASE COUNT         8530 a         9321 c         8519 g         8088 t
ORIGIN      
        1 accttcaacg ttgagtgttg tactattttg tttttaatcc actatattct gactgcctca
       61 aagggcaaaa ataatgtgag tatgttgagg ctgcctcttt ttgaaatgac aaacaaaatg
      121 ccgtctgaaa cccgatttcc ggtttcagac ggcatttgat gttcaatcag ttgtcaaacc
      181 tgattatgcc aaaccttttg ctttcaacac ttccagcatc gtcgtgccca actcggcagg
      241 gctgcgggtg taggcgatac cggctttttc aaacgcggcg aatttttctt cggcagtacc
      301 tttaccgccg gagataatcg caccggcgtg gcccatgcgt ttgcctttag gagcggtaac
      361 accggcgatg taaccgacaa caggtttggt tacgttggat tggatgtatt cggctgcttc
      421 ttcttccgca gtaccaccga tttcaccaat catgatgatg gcatcggtat ctgggtcttc
      481 ttggaagagt ttcagtgcgt cgatttggtt cataccagga atcgggtcgc cgccgatacc
      541 gatacaggtt gattgaccca agcccagttt ggtggtttgt gccacggctt cgtaagtcaa
      601 tgtaccggaa cgggaaataa taccgatgcg gcccggagtg tggatgtggc ccggcataat
      661 gccgattttg cactcgcccg gagtaatcac gcccgggcag ttagggccga ccaaacgggt
      721 accgttgccg ttggtttcca agtaacgttt ggctttgagc atgtccagag ttggaacgcc
      781 ttcggtaatc acaacgacca agcctacgcc tgaatcaacg gcttcaacga tagaatccaa
      841 cacaaacgga gcgggaacgt aaatcacgga tgcgtcagcg ccggtttctt taacggcttc
      901 tttcatggtg ttgaatacag gcaggtctag gtgggtttga ccgcctttgc ctggggtaac
      961 gccgccgaca actttagtgc cgtaagccag agcttgttcg gagtggaaag taccgttttt
     1021 accggtgaaa ccttgaacca atactttggt gtctttatta atcaatacgc tcattctttt
     1081 ctccttaggc gtttacggct gcaacaattt tttcggctgc gtcattcaga ccgtctgcag
     1141 aagtcagttt cagacctgat tcgttcagga ttttcgcgcc gagttcggcg ttgttgcctt
     1201 ccaaacgaac aacgacagga acgttgacgt tgatttcttt aacggctgcc acgatggctt
     1261 ccgcaatcat gtcgcaacgt acgataccgc cgaagatgtt gatcaatacg cctttaacgg
     1321 atttgtcttc caaaatcagt ttgaacgctt caaccacgcg gtctttggtt gcgccgccgc
     1381 caacgtccaa gaagttggca ggttggccgc ctttgagttt gatgatgtcc atggtggcca
     1441 tcgccaaacc ggcaccgtta accatacagc cgatattacc ttccagggca acatagttca
     1501 ggtcgaattc agaagctttc agttcacgtt cgttttcttg agatttgtcg cgcaattcag
     1561 cgatttttgg caggcggtag agcgcgttgc tgtcgatgcc gattttgccg tccacgcaag
     1621 ccagtgcgcc gttttcgcga actgccagcg ggttgacttc aaacagggcg aagtcgtttt
     1681 cgacaaacgc tttgtacgcg ccggtcatca gtttggcgaa ctcgttgatt tgtttgtctt
     1741 tcaagcccag ttggaaggca acttcgcgag cttggcaagg ttgcaggccg accagcggat
     1801 caacggttac tttgaagatt ttttctggag tttcagcagc aactttttcg atttccacgc
     1861 cgccttcggt agaagccatg aatgtaacgc ggcgggtaga acggtcaacc actgcgccca
     1921 agtacagctc ggtttgaaca gggtacatgt cttcgcaaac caaaacgctg ttgacaggtt
     1981 ggccgttggc atcggtttgg taagttacca agttggtgcc aatcaggctt tcagccactt
     2041 ctttggcttt ttcgcggctt ttaacgactt ttacgccgcc cgctttaccg cggccgccgg
     2101 cgtgtacctg tgctttgaca acagcgaatt tgccgcccaa tttgtcgtaa gctgcagcgg
     2161 cttcttcgcc gttgtgcgcc aaaataccgc cttgtacggg caaaccgtag ctagccagca
     2221 gttctttagc ctgatactcg tgtaaattca tggatttctc cttaaggtga gtgggtttta
     2281 ggaatcgccc tgcggccaat cccatatttt cagacggtcc tactatttac tgaagggcaa
     2341 ccacatccgc gccagcagat acatgactgc accgcctccc ccgagaataa aaaagacgat
     2401 gcctttttta cgcgaattcg gacagagcag ataaacggca aggctgaaac tgataaacaa
     2461 caatgccacg cgccaaacca attctttatc tttgaaacgg ttgagcgtat aagtttcggt
     2521 catcatcaca tacatctgcc acaatgcagc catacacatg ctgatgatgc ccatcggtat
     2581 aaagaaaata ccgactattt ctttgttcat aatgaatgta aatgctgttt gtctgacgga
     2641 attataaatc agaaaacgaa gctctcggaa cattcaattt gccggaggcc ttaaggccgt
     2701 ctgaaaattt cagacggcct taatgtctat caaccgtgca aagcgcgttt gtcggccgcc
     2761 aatgcagctt cgtgaaccac ttcggacaag gttgggtggg catggataat acgggcgata
     2821 tcttcgctgc tggcgaagaa ttcgagcgca gtcacgcctt cggtaaccaa ttcgctgaca
     2881 accgggccga tcatgtgtac gcccaagata cggtcggttt tggcatctgc caacacttta
     2941 accgtacctt tggctttgcc cattgccaat gcacggccgt ttgcaccgaa acctgaagta
     3001 ccttttttgt actccacgcc ttcggctttg agctgctctt cggttttacc cacccaagcg
     3061 atttcagggt cggtgtaaat cacaaacggt acgttgttga agtcgatatg cggtttttga
     3121 ccggcaatgc gctcggcaac ggcaacgcct tcgtcgctgg ctttgtgtgc caacatcggg
     3181 ccgcgaacca cgtcgccgat tgcccatacg ttaggcaggt tggtacggca ttcgccatcc
     3241 actttgataa agccgcgctc gtctttttcc aagcctacgg cttcagcgtt caggcctttg
     3301 gtgtttggaa tacggccgat ggcaacgatc agtttgtcga atacttcggt tttggcttcg
     3361 ccggcagcag tttcgtaagc aacggaaaca cctttgcctt cagacttgat gtcgccgatt
     3421 ttcacaccca attcgatgct cagaccttgc tctttggtga agtatttgaa ggcttctttg
     3481 gcgatttgtt ggtcggcggc agccaggaag gtcggcgcgg cttcaagaat ggtaacttcc
     3541 gcacccacgc ggttccatac ggaacccatt tccaaaccaa tcacaccgga accgattacg
     3601 ccgagtttgg caggtacttc ggtcaggttc aatgcgcctt cgttgtccaa tacgttcacg
     3661 ttgtcgatag cgacttgcgg caacggacgc ggtacggaac cggttgctac gatgacgtgt
     3721 ttggcttcga taacggtttt ctcgcctttg ttatcgactt caatttggta agcatcgcca
     3781 tttttaccgg caaaggaagc agtaccgaac aggctggtta ctttgttttt ttggaacagg
     3841 aatttcacgc cgccggtcag tttggtcacg atggcatctt tgcgctcaat cattttggcc
     3901 acgtcaaatt ttacgtcgcc gacagtgata ccgtgttcgg caaaatcgtg ttgcgcagcg
     3961 tggaaatgtt cgctggattg caacagggct ttagaaggga tacagcctac gttcaagcaa
     4021 gtaccgccca atgcaggggc attgcctgct ttgttaacgc ctgcatcgac acaggcagtt
     4081 ttgaaaccca gttgtgcggc acggatggcg gcaacgtatc cgcccggacc tgcaccaatc
     4141 actactacat catattgaga catcgtgtca tcctttgctt ttagcagtat ctcaaaataa
     4201 aaacgatact tttcgtttct ttttttattt tgaatgactg cgttttgatg gttttaagaa
     4261 aaagccgtct gaaagtccat atctgccgat tcattcgaat caaatgtttc agacggcctc
     4321 aatcgttcgt tgagcctatt gtagcacgat actacattac gctacatcta aataaaaatt
     4381 cagacggcct tttcattaac aaataaaaag ccgtctgaaa cgattacaga tccaacaaca
     4441 ggcggaccgg gtcttccaac gcgtctttaa tggctaccaa ggtcaataca gcttcgcggc
     4501 cgtcaatgat acggtggtcg taagacagag ccagatacat cattggacgg acaacaactt
     4561 ggccgttttc aaccacagcg cgctctttag tggcatgcat acccaaaatc gcagattgag
     4621 gcgggttgat gatcggggtg gacatcatag aaccgaaagt accgccgttg gtaatactga
     4681 atgtaccgcc ggtcaggtct tcgatagcga ttttgccgtc tttggctttt ttcgcgtaat
     4741 caacaattgc ttgttcgatg tcggcaatgc tcatttggtc ggcatcgcgc aggattggta
     4801 caaccaaacc gcgtgggctg ccaattgcga taccgatgtc gaagtagccg tggtacacaa
     4861 tgtctttgcc gtcaacagaa gcattcacaa ccgggtattt tttcagggct gtaacagcgg
     4921 ctttaacgaa gaaggacata aagcccagtt ttacgccatg ttctttctcg aatttttctt
     4981 tgtacttcgc acgcaagtcc atgatcggtt tcatgttgac ttcgttgaat gtagtcagaa
     5041 tggcgttttc ttgttgagaa gccaagaggc gttctgcaac acgggcacgc aggcggctca
     5101 ttggtacgcg ttcttcagga cgtgcgccgg caggaagtgc aacggcaggg gccacggctg
     5161 cggcaggttt ggcagcggca ttttgtacgt cttctttcaa tacgcgaccg tcacggccgg
     5221 aaccttgcaa tacgttcacg tcaacaccgg tctcggcagc cagttttgca gcggcaggca
     5281 tggcggcgtt gttttgtgca gcagcaggaa cggcagccgg ggcagcttcg gcaggagcgg
     5341 ctgcaggtgc ttcagcagcg acagtagcag ctgtatcgat acgggccaaa acttggtcgg
     5401 caacaacggt ttcaccgtct tgcgctacga tttcaaccaa tacgccggct tgtggagaag
     5461 gtacttccaa aaccactttg tccgtttcga tatcgatcag gatttcgtca cgggcaactg
     5521 cttcgccaac ttttttcttc cattccaaga gtgtgccttc agatacgctt tcagacagca
     5581 taggtacttt tacatcaata atcattttgt gtctccaatg gccctttcag acggcctgtt
     5641 gtgtgttttt aattctgcat atgggcattc cgcctgagca gactcagacg gaatgctact
     5701 tggtttataa cgccaaagcg tcttcaacca attgtttcaa ttgagcaatg tgtttgcttg
     5761 agtagcccac tgcaggcgat gcgctgcttg gacggccggc ataagacagt ttttgctctt
     5821 cgctgataac gtcttcgatg cggtggcgga tttggtagaa cgcgccttgg tttttcggct
     5881 cttcttgcgc ccaaaccaca gattttgcgt tcggatattt cgccagttcg gctttaacct
     5941 cgtcgtatgg gaacggatac agctgctcaa cgcggacgat agcgacatca tcttccagtt
     6001 tacgttcggc tcgaccggct tccaagtcat agtaaacctg accggcacac aataccacgc
     6061 gtttcacgct gtcgttgctt gcgcgttcgg cggtatcacc aataaccgga cggaaagtcg
     6121 aaccttcggt gaagttttcc agcgggctca ttgcaccttt gaagcgcaac aggcgtttgg
     6181 acatgaaaat caccagcggt ttgcggtatg aacccaagac ttggcgttgc aagagatgga
     6241 acatttgcga cgcttcagac ggcatgatga cttgcatatt gttctcagaa cacagttgca
     6301 accaacgttc tacgcgtgca gaagagtgtt cagggccttg accgtcgtag ccgtgcggca
     6361 ggatggtggt cagaccgcac aagcgacccc acttggtttc gcctgaagac aggaattgat
     6421 caatagttac ttgcgcgccg ttggcgaagt caccgaattg agcttcccaa atggtcagct
     6481 tgtcaggagc ggagcaggca aagccgtact cgaacgccat cacggcttct tcgttcaaga
     6541 tagagtcgat aaccaggaac tcgcccaagc cttcgcccat gttgcgaaga ggaacataag
     6601 taccgtcgtc ccatttttcg cgtttttgat cgtgcagaac ggcatgacgg tgtgagaatg
     6661 taccgcggcc ggagtcctca ccggagatac gcacgccatg acctttggtc agcaggcttg
     6721 cgtatgcgag ggtttcggcc ataccccagt cgatggcttg tttgccggat gccatggctt
     6781 tacgcgcttc gatcacacgt tttgcagtcg gatgcagggc aaagccttcc ggcacagcgg
     6841 tgaatttctc agtaagacgc tcaatgtcgg cggcaggcaa accggtttcg attttttcgc
     6901 gccaatcttt accttggtat ttgctccagt cgatttgtgt gcgttggaag ttgctcaacg
     6961 ttgtttgctc aacatgttcg cctttgtcca aagcatcacg gtaggcttgg atgtaaccgt
     7021 cggcctcaac ttgagttacc acgccttcgg caatcagttg ctcggtgtac aaagcacgcg
     7081 cacccggatg ttgcgatact tttttgtaca tcatcggttg ggtcaaagtc ggatcatcgc
     7141 cctcgttgtg accccattta cggtagcaga caacgtcgat tacgatgtct ttatggaatt
     7201 ttttgcggta atccaaagcg gcttgaatgg caaagcaaac gcgttcggga tcatcgccgt
     7261 tcacatggat aaccggagcg gaaaccattt ttgcgatatc ggtacagtgt acggttgaac
     7321 gggtatcgcg gatatcggaa gtggtaaagc cgatttggtt gttgatgacg atatggaccg
     7381 taccgcctgt ggtataaccg cgcgttttag acaggttgaa tgttgcttgg ttgacaccca
     7441 aaccgataaa tgcggaatcg ccgtgaatca ataccggcaa gactttatcg cggccgtttt
     7501 cgcccaaacg tttttgtttg gcgcgtgcag aaccttccac taccggattg acgatttcca
     7561 agtgtgacgg gttgaacgcc aaagaaacgt gcatcgggcc gtgaggggtg gcaatatcgg
     7621 agctgaagcc catatggtat ttcacgtcgc cactaggcag tttgatttcg gcacggcctt
     7681 caaattcggc aaacaaatcg ccgggttttt tgcccaaaat gttcaccaaa acattcagac
     7741 ggccacggtg cgccataccg atgatgactt cttccacgcc gtccttaccg gcgttttgaa
     7801 tcaggtagtt caaaccggca atcgcgcttt cgccgccttc gacaccgaaa cgtttctgac
     7861 cgacatattt ggtatgcaga taacgttcca aagtctcggc agcggtcatc tctttcaaga
     7921 tacggcgttt ttgatcggca ttgtaatgcg gtgtggacaa tacgctttca aaatagttgc
     7981 gtacccaacg gcgctcttcg gtattgggaa tatagatata ttccaatgcg atgtggccgc
     8041 agtaggtttg tttgaggttg ctgatgattt gggacaaggg cagtttgccg cgattggcaa
     8101 aatcaccctc gcccatattg aatcgaagcg ccatatcggc atctgacaga ccgtggaatt
     8161 tcggatcgag ggcttcaata tcgcgcggag ggatacgttt gagcggatca agttgggccg
     8221 cacccacgcc ttggatacga taggcagaaa tcagccgtaa aacgctgact tgctttttca
     8281 gcattgcctc atccgcaccg cccgcaacgg cagatgcaat tttctttttc gccaaagtaa
     8341 caaatgattc gcgaatcggt gtgtgtgcga catcgacagc aaccgtcccc ggctgtttgc
     8401 tcaaatcggt gaaatactgc ttccattttt catcaaccgc atcggggttt tccaaaaaag
     8461 cctcgtacaa ttcctcaatg tagggtgcgt tcgaaccgaa cagataagag aaattgagtt
     8521 tttcgtccat catggcgtgc gctcttttct ttaaaatata aacaaagggt agaagtccgc
     8581 gtcctgacgg ctgaactgat gccgtctgaa aagacatttt attctaccct attttaaacc
     8641 ggattgcttg tcttgatttc atccgactac aatcagacgg attcaggaaa caatccggtt
     8701 ctctgatatt gcctgtttga caatatatta atgtatgtta cctctcgcct gccggcacat
     8761 aatcgcggcg ttccgaaccg gtataaagct ggcgcgggcg gccgattttc agcgaaggat
     8821 cgctaatcat ctcgtgccag tgcgaaatcc agcccacgct gcgcgacagg gcgaagatga
     8881 cggtaaacat ttcggtcggg atgcccagcg cggacaggac gatgccggaa tagaaatcga
     8941 cattgggata cagtttgcgt tcgataaaga acgggtcttt cagcgcaatc tgttccaatt
     9001 ccatcgccag tttgaatttc ggactgtctt ccaagcccaa ttccttcaaa acttcatagc
     9061 aggtttcgcg cataatgctg gcacgcggat ccatattgcg gtatacccgg tgtccgaagc
     9121 ccatcaggcg gtatttgcgc tgtttcacgc cttccatgta tgcggcaaca ttggacacat
     9181 cgccgatttc atccagcatt ttcaacacgg cttcgttcgc accgccgtgc gaagcacccc
     9241 acaggcaggc gataccggca gcaatacagg caaacgggtt cgcacccgaa gagcctgcca
     9301 gacggacggt tgaagttgag gcgttttgct cgtggtcggc atgcaaaata aagatgcggt
     9361 cgagcgcgcg tgccaaaacg ggattaggtt tgtagtcttc acacggtgtg gcaaacatca
     9421 tatgaaggaa gttttctgaa taagaaagat tattcttcgg ataattgaac ggcagaccgt
     9481 ttgaatagcg gtaacacatt gccgcaatgg tcgggatttt ggaaataagg cggtaaatcg
     9541 cgattttgcg gtgttcggga ttagtaatgt ccaagctgtc ttggtagaac gcagacagcg
     9601 cgccgaccac gccgaccatc atcgccatcg gatgcgcgtc gcggcggaaa ccccggaaga
     9661 accaagtcag ctgttcatgc accatcgtgt ggcggcggac tgtattgtca aattctgcct
     9721 tttgctcggg agtcggcagt tcgccgtaaa tcaacaggta gcagacttcc aaataatcgg
     9781 acttttcggc cagctgctcg atggggtatc cgcgataata aagcaagcct tgatcgccgt
     9841 cgatgtaagt aattttagac tcacagcttg cggttgaaac aaatccgggg tcgaaggcaa
     9901 acaaacctgt attttttgtc agcccccgaa tgtcaaccac atcatgcccg atgctggctt
     9961 ccaataccgg cagctccaaa cctgcccgac ccggtacgtt gagtttgatt gatttggaca
    10021 tattatcgct cctttgcaag ttataaccca cgttactgca tccgcagcac gttctttgct
    10081 ttcagacggc ctttgcattt tgaaatctgc cctcagacaa atttcagatg cctcatgccc
    10141 gtctgatttt ttcaagcatc gggataaggt gccctttgtc cgtttccgaa tgcccgtgaa
    10201 tcaaggcaag caattcttga tcttgaaatt caaggatttc ggaaaactcg gacagctctt
    10261 tatcgctcaa atgctcgaat tctttttcca taaacctgcc gaagattaaa tctagttcca
    10321 acaatccccg gcgggtttga aaacggattt tccgtttggc aatgtcgtca aaaaccatca
    10381 tttcttaaac aacccgtttc aacataatct ctttaatctt accgatggcc cgggtcggat
    10441 tcaagtgttt aggacatacg tccacgcagt tcataatggt gtggcagcgg aacaaacggt
    10501 acggatcgtt caaattatcc aaacgctcat tagtgatggt atctcggctg tccgcaatga
    10561 agcggtaggc gttcagcaag ccggacggac cgacgaattt gtcgggattc caccagaatg
    10621 acggacaggc ggtcgaacag caggcgcaca aaatacattc gtacaaacca tccaactctt
    10681 tgcgctcttc ctgagtttgc agacgctctt tgtccgcatc aatcggattg tcgttaacaa
    10741 cataaggttt gacggaatgg tattgtttga agaactgggt catatccaca atcaggtcgc
    10801 ggatgacggg cagaccgggc agcgggcgga ttttgacagg ctgtttcaag ctgcgtaaat
    10861 cggtcagaca cgccaagccg tttttgccgt tgatgttcat accgtccgat ccgcaaatcc
    10921 cttcgcggca ggagcggcgg aaagacaagg tatcgtcttg tgctttcagg cgtaccaaag
    10981 cgtccaaaag tttcacgtcg gtcggttcca attccaactc gtaacgctgc atataaggct
    11041 tggcatcaac gtccgggttg taacggtaaa tttcaaaact cattttttcc atgagaagtg
    11101 ttccttttct catcaactgc ccaatgattt tgtattataa attcaagcag ttcaatgtgg
    11161 gtatggttag gtttcagacg acctttgagg cgaagaccgt ctgaaaacgc atcaataaac
    11221 gcgtttggcc ggtttgatgt attccacgct caaaggcttg gtgtgtaccg gtttgtaaga
    11281 caaggtattg gtgtccgaat gatacagggt gtgtttcatc caattttcgt catcgcgctc
    11341 aggatggtcg tctgaagcgt gcgcaccgcg tgattcttta cgtgcttcgg cagacaccaa
    11401 agtcgctttc gccacttcaa tcaggttatc caattccaaa gcttcgatac gcgcggtatt
    11461 ccacactttg ctcttgtctt tgatttcggt acgtttcaca cgctcggcaa tcgccatgat
    11521 ttcttgaacg cctttgctca gaatctcatc ggtacggaac acgccggcgt gcagttgtac
    11581 ggagcgttgc agttcgcgac gcaatgcatc aacgttttca ccatcggttt gactgtccaa
    11641 acgctcgata cgttggcggg tcaactcacc ggcattagca ggcagaggtt tccaatcgct
    11701 ttgctctttg atgaatttaa tcatgctgtc accggcagct ttgccgaaca ccaccaagtc
    11761 cagcagggag ttcgtaccca aacggttcgc accgtgtacg gaagcacagg cgcactcacc
    11821 tgcggcatac aggcctttta caggtacttc gtactcgtcg ccttgcggaa caacaacttc
    11881 accgtgataa ttggtcggaa tgccgcccat catatagtgg gtagtcggca caaccggaat
    11941 cgggtcttta atcggatcga taccggcaaa ctgaatggaa atctcgcgga tgcccggcag
    12001 tttttccata attttttctg caccgatatg gtcgattttc agtaagacgt ggtctttgtt
    12061 tttaccacag ccgcgacctt catagatttc catcgccatc gcgcgtgaaa ccacgtcgcg
    12121 agaagccaag tcttttacgg tcggcgcata gcgttccata aagcgttcgc cgtcggcgtt
    12181 caacagaata ccgccctcgc cgcgtacgcc ttcggtaatc aacacgcccg cacccgccac
    12241 gccggtcggg tggaattgcc agaattccat atcttccaac ggaatgcccg cacgggcgca
    12301 aatgcccaaa ccgtcaccgg tattcatata agcattggta gaagaagcat aaatacgacc
    12361 gccgccaccg gtagcaaaca tcacggcctt ggcgtggaaa atataaactt cgcccgtttc
    12421 catttccatg gcggttacgc cgacgacatc gccgttttca tcacgaatca aatcttgcgc
    12481 cgtccattcc acaaagaatt gtgtattggc acggacgttt tgttggtaca aagtatgcaa
    12541 catcgcatga ccggtacggt cggcaaccgc acatgcacgt tctaccgcac gtttaccatg
    12601 ttcggcagta tgtccgccga aaggacgctg ataaattttg ccgctttcaa cgcggtcaaa
    12661 aggcataccc atgtgttcca actcaatcac cgcttcaggc gcagcgcgac acataaactc
    12721 aatcgcatct tggtcgccca gccagtcgga acctttcacg gtatcgtaca tgtgccagtc
    12781 ccaacggtcc tcctgcacat tacccagaga ggcggaaata ccgccctgcg ccgctacggt
    12841 atgcgagcgg gtcgggaaca ctttagacaa aacggcacaa ttcaaaccgg atttggataa
    12901 ttggagggct gcacgtaaac ctgcaccgcc accgccgaca atcacggcat caaacttgcg
    12961 aacaggaaaa cccatactta cccccaaatc actttaactg aatacacgag gcagccgacc
    13021 agccagacaa tggtggcaac ctgcaaaaac aaacgcacgc cgaagggttt gatatagtcc
    13081 atccacaaat cgcggatacc cacccaagcg tgcaagaata cggcgataaa gctcacttgg
    13141 gtaaatactt ttacccaagc ttgactaaaa aatgcctgcc atgccggata ttctttaggc
    13201 agggcaaata gaaccactaa aagtgcaacg gtataaatca acataataac cgcagtcgca
    13261 cgctgcatta cccaatcgcg caaaccgtaa tgggcaccgg tcaatttacg ttctaccata
    13321 acaacgctcc caaaacgaca gtcaaaacca atgcagaagc aaatacggct ttagcggtat
    13381 tgcgcgcagt attcagctca aggcctttgt gcgcatccaa aaataaaaag cggataccgg
    13441 cgagagaatg gtgcagataa gcccacaata taccgattaa aaccagcttg accaaaggat
    13501 gggaaacaat ggcacggtaa gtttcaaatg cagactcttg actcagggta ccggacagaa
    13561 aatacagcag gaaaggcagc ataataaaca gcccgacccc gctaatgcgg tgaaggatgg
    13621 aaactatccc gggtatcggc agacggatgt tcggcaaatc cagataaaca ggacgcagtt
    13681 tgacagacat agtcaattcc tctgtagtta tatatcgact caaaaaccgt aaacccattg
    13741 ctacactggg ttacatcaac gttaatttac ctgttttatc ccattttgaa cagtgtattt
    13801 taaaaacaaa ctgatagatt ttgacaaaca cgcgtggaaa cagcagatta aaagaaagat
    13861 ttttagtttt gccgctccct tcccgacacg gtatcagcat ccgaccccca accccatccc
    13921 caaaaataaa atgccgtctg aaaaaaattt caggcggcat tgttttgcca tatcggtcag
    13981 gctttcaatt tcgcatcggc atcgcgcaac gcggtacgca gaccttcctc gataacagga
    14041 tggtagaacg gcatatccag catttgcgga acggtcatct tcatttgatg cgcccatgcc
    14101 aacaagtgag ccaaatgttc ggcggcagga cctacgattt ccgcgccgat aaagcggccg
    14161 gtggcttttt cggcatacag gcgcatatgg cctttgttta ccagcatcac gcggctgcga
    14221 ccttgatttt tgaacgatac ttcgccgatg acaaattcgt cggtttggta ttgcgcggca
    14281 acctgcgcgt atttcaaacc gacaaagccg atttgcggac tggtaaacac cacgccaatg
    14341 gtgctgcggc gcaaaccgct gccgatattc gggtagcggc ccgcgttatc gccggcaatc
    14401 ttaccttggt cggcggcttc atgcagcagg ggcagttggt tggacgcgtc gcccgcaata
    14461 aagatatgcg gaatgctggt ctgcatggtc agcggatcgg caacgggtac gccgcgcgcg
    14521 tctttgtcga tattgatgtt ttccaaaccg atattgtcaa cgttcggacg gcgacctacg
    14581 gctgccaaca tatattcggc aacaaatacg cctttttcgc catcctgctc ccaatggact
    14641 tctacattgc cgtctgcgtc gagtttgacc tcggttttag catccagatg cagtttcaat
    14701 tcttctccga acacggcttt cgcctcgtct gaaacaacgg ggtcggaaat gccgccgatg
    14761 attccgccca aaccgaaaat ttcaactttc acacccaaac ggtgcaatgc ctgacccagt
    14821 tccaaaccga taacgcccgg cccgaacacg gcaacgcgct taggcagcgt atcccacgag
    14881 aaaacgtcgt cgttgataat caaacggtcg cccaaagact gccactgcgg caggatgacg
    14941 ggacgcgaac cggtagcaat cacgaaactt tttgccgcaa tttggatatg gtcgtcgatt
    15001 tggacggtat gctcgtcgat gaatttagcc gagcccatga tgcgcttgtc ggcaggccat
    15061 tcttccacat cggtaacgac aaagccgaca aaacggtcgc gctcggattt gacgcgccgc
    15121 ataacctctt ctccgttgac gacgatgctg tctttgtcca aatgcacgcc gaaggggtcg
    15181 gtatgcaatg cgtggtgacg cgcttctgcg gcggcaatca agagtttgga aggcatacag
    15241 cccacgcgcg cgcaggttgt gccgaacaca ttgttttcaa tcaggtaaac attatccgaa
    15301 tgtaaacggg cattgcgaaa cgcacccata ccggcagtac cgccgccgat tacgacgata
    15361 tccgcttgaa tttttttcat aatctttgtc cttccgtttt cgtctgaaac attgccgaca
    15421 cagaacatat cggtttcaga cagcatttaa atatcttccc aatcaaaaac attattcaga
    15481 taaataaaaa caaaatttcg attgagtgcg acaatatcca aatcttcttg taaatataga
    15541 gttattcgga gcggcagtat agaaccgccc gaataactct atccggtata tcagacagtt
    15601 taatcaaact gccttaagca aacagctatc agtttttagc caagtaagct tccaaatctt
    15661 cgctgccgcc gatgtatttg ccgccgatga agacttgagg ggcagtcatc ttgccggtaa
    15721 tagcgcgaac ggaagtaacg gttgcatctt tgcccaatac gatttcttcg taagacaaac
    15781 ctttgtcttg caaagcttgt ttgactttgg cacagaattg gcaacctggt ttagtgaaaa
    15841 ttgccacaga ctcttgagcc ttccaatcgg gagcaacgaa tttcagcata gtatctgcat
    15901 cggatacttt gaaaggatcg cccggttctt caggttcgat gaacattttt tcaaccacgc
    15961 cgtcgttaac cagcatggag taacgccaag agcgtttgcc gaagcccaag tcttctttac
    16021 cgaccagcat acccatacct tcggtaaatt cgccgttgcc gtcaggaatc atgtagatgt
    16081 tgtctgattc ttcttcggca gcccaagcgt tcattacgaa agtatcgttt acagatacgc
    16141 agcagattgc gtcaacgccg ttttctttga acgcgccgaa taattcgttg taacgcggca
    16201 ggtgtgaaga agaacaagtc ggggtaaatg caccgggcag ggagaatacg actacttttt
    16261 tgcctttgaa caaatcatcg gtagacacat ctttccaagt gtcgccgacg cgggtgcgga
    16321 atactacgga aggtactttt tgaccggtac gatcttgcaa agccattttg agctccttaa
    16381 tagattggtt tgagaaataa ttgtgaatcg ccgcgcattc taccccatgc aaaatgattt
    16441 gtataattta tagtttaaat aatataaatt atttaaaact atcaaataca actccgattt
    16501 gcctgttccc cacatataaa gccgaattag agaaaaacaa gctgcaatga aaagattttc
    16561 cggcaactgc tcccctgcgc cgcaactcag gacaaaatgc cgtctgaaag gctttcagac
    16621 ggcatttgtt catacggtct cggcttattt cgccaattcg gcacgcagtt tgcgggttac
    16681 gttcatcatt acttgaagct gttccagagt ttctttccag ccgcgtgttt tcaggccgca
    16741 gtccgggtta acccacagac gctcaaccgg aacaacctca atggctttgc gcaacaggtg
    16801 ttccacttcg gcttctgtcg gtacgcgcgg gctgtggatg tcgtaaacgc ccgggccgat
    16861 gtcgttcggg tatttgaatt cgccgaacgc agccaagagt tccatgtcgg aacgtgaagt
    16921 ctcaatggtg atcacgtccg catccattgc agcaatcgca ggcaggatgt cgttgaactc
    16981 agagtagcac atatgagtgt ggatttgagt gctgtcttcg caacctgcag aggataggcg
    17041 gaaggattcg cccgcccagt tcaggtaggc atcccaatcg gcacgtttca aaggaagacc
    17101 ttcgcggatg gcaggttcgt caatttggat gactttaatg ccggcttttt ccagatccaa
    17161 tacttcgtcg ttcagagcca gtgcgatttg tttgcacacg gtagcgcgcg gaatatcgtt
    17221 gcggacgaaa gaccattgca gaatggttac aggaccggtc aacatacctt tcatcgggcg
    17281 tttggtcagg ttttgtgcgt aagtagacca agcgacagtc atagcttcag gacggcttac
    17341 gtcaccgaag atgataggtg gtttaacgca gcgtgagccg tagctttgca cccagccgta
    17401 ttgggtgaat gcaaaaccgc tcaacagttc gccgaagtat tcgaccatgt cgttacgctc
    17461 ggcttcgccg tgcaccagta cgtccaagtc cagtttttct tgctcttcaa ccaccaaggc
    17521 gatttctttt ttcattgcgg cttcgtaatc ggaggcagac agttcgcctt ttttgaaggc
    17581 tgcgcgtgct tggcggattt cggtagtttg cgggaaagaa ccaatgttgg tcgtcggcag
    17641 cagaggcagg ttcaaccatg cttgttgcgc tttgatacgg tcggcaaacg gtgatttgcg
    17701 ttggtctgcg ttgacaggca ggtcggccaa gcgtttggca acgtctgcac ggtggatttc
    17761 actgctgttg gcacgggagt cggcagcagc ttggctggcg gccagttctt cggcaacaga
    17821 atcacggcct tcattcaatg cggctttcag aacacgcaat tcttgggttt tttgcagagt
    17881 gaatgccaac caagagtaca ggtcgggttt gttggctttc agtttttctt cgactgacaa
    17941 gtcaaacgga gtgtgcagca gcgagcaaga gctggaaatc cacaaacgct cgcccagttt
    18001 ggcttgcaga ggcccgacgg tttccaaaac tttgttcagg ttggcgcgcc aaatgttgcg
    18061 gccgtcaatc acgccggcag acaggacttt gtcgtagccg gcgaacgcgt ccagttgctc
    18121 gggtgcgcgt accaagtcga tgtgcaggcc gtcaacaggc agggatttca acaatgcggc
    18181 gtgttcggca acagaaccga agtaagtgcc caacaggatt ttggcgctta ctttgttcaa
    18241 agtagcgtaa acgtctttgt atgcttctac ccattcttta ggcagatcga cagccaaagc
    18301 aggctcgtcg atttgaatcc actcggcacc ggcttcaacc aaagcagtca ggatttcaac
    18361 gtaaacaggc aacagtttag gcaacaggct cagacggtcg aattcaacgg agcctttttc
    18421 tttacccacc cacaggaaag tcaaaggacc gacaacggtc ggtttggctt tcaagcccaa
    18481 agtttgggct tcttgcagtt gttgaacgta gtgtttggcg ttggctttga attcggtatc
    18541 ggcatggaat tcaggcacca ggtagtggta gttggtgtcg aaccatttgg tcatttcgat
    18601 ggcgaattgg tctttgttgc cgcgcgccag ttggaagaat tgttccaaag gcaggttttg
    18661 gctatcgaag ccgaaacggg cgggaatcgc gccggtggca acttgcaggt cgaggatgtg
    18721 atcgtagaaa gtgaaatcac ctacagcaac gtaatcagcg ttggcagcag cttggtgttt
    18781 ccagtttttc tcgcgcaggt ctttggcaac gtcgagcagc tcttgttcac tgatctcttt
    18841 gcgccagtat ttttcttggg cgaatttcaa ttcacggaag gcgccgacac gcgggaagcc
    18901 tgagaaatgt aatgttgtca tgttgaactc tccagttgga ttttgtaagg accggttcaa
    18961 tccgaatcgg ccggtattgt aaatcagaat ggctcggata gggcggcgtt ttgcttttca
    19021 gacggcctcg gatgctgtct gaagccgttt tcggctttaa gggcgcgcgc ctaaaatatg
    19081 gcagatggcg taagtcagct cgctgcggtt gagcgtgtag aagtggaaat ctttcacgcc
    19141 ttcgcgggac aggactttaa ccatatcgat ggcgatactg gcggcgacga ggttgcgcgt
    19201 gccttggtcg tcgtccaagc cttcatacat ttgcgacagc catttcggga ttttgacgtt
    19261 ggttacttgt gccattttgc cgagttgctt gaagttggta acaggcagga tgccgggaac
    19321 gatttcgacg tcgatgccca acatcacgca gcggtcgcgg aagcgcaggt agcgttccac
    19381 atcgaagaag aattgggtga tgacgtggtt cgcgcccgca tcgattttgc gcttcagatt
    19441 aatcaggtcg gcttgtgcgg atttcgcttc gggatgtact tcggggtatg ctgctacaga
    19501 gatgtcgaag tcggcgacgg agcgtaatag cttaaccaag tcttcggcgt aaaacggttt
    19561 tttctcataa ccggccggct cgtcgccacg cagggcaacg atgcggcgga taccgctgtc
    19621 ccaatagtct ttggcgattt ggcgcaattc gtcgtgggat gcatcgatgc cggtcaggtg
    19681 cggagctgct tccaagccgg tttcctgttt gatgcgtttg acgatgccgt gcgtgcggtc
    19741 gcgttcgccg gagtttgcgc cgtaagttac ggatacgaac ttgggatgca gggtttgcag
    19801 acggtggatg gaatcccaca gcatcgtttc catttgttcg tttttcggcg ggaaaaattc
    19861 aaacgaaacg ttgatgttgc ctttcaaatc ggaaaggctg ttgtttaacg cattgatttc
    19921 ttttgcataa tttatctgca taccttcgtt atcttttaca tgatgtttca ggcggtatga
    19981 taaggttgca ccgaatatga gtcaatttca aaattttcag gtttatgatg aaaattttta
    20041 atatttagca aaaactatac gcttttcttg tacaagcctt ctaaattatg ataaagtttg
    20101 ttatgttata acacttcaat caaggcaata tatgaaaccg aatattcatc ctgacaatta
    20161 tcgaaccgtc ctttttttcg acagcagtgc caatgaaggc tggctgattc gttcttgcgc
    20221 cggaacgcac ggaaaaacaa tggtttggac agacggcaaa gaatatccgc tcttttcctt
    20281 agatacttcc tcttcctcac atcccgtcta taccggcaaa cagcgtaacg tcaataccga
    20341 aggccgtgcc agcaagttca accaacgttt ccaatccgtc atgtcatcgt ttagaaagga
    20401 caaataatgc aggttctgtc ttcgctcaaa accgccaaac aacgccaccg cgactgccaa
    20461 atcgtcagac gcaggggcaa agtgtacgtc atctgcaaaa gcaatccgcg ttttaaatcg
    20521 cgccaacgct gaccccctgc ccttcctgct tcggttgtaa agcccctcct accgcaagcc
    20581 cttttcaaat atgccgtctg aaacagaaaa atgcgtttca gacggcattt ttccgtcgat
    20641 acgaatccgg aatgtgtttg aaatacagca taaacagata ccaaaacagc gtcgcggcgg
    20701 ctgcaccgag cagtacccat gcgctgattt cggcgacttc gcgcaggaca cgtttctgcc
    20761 tgcggctaaa gaggcggttg ataatcagcg cggcaagtgc gaaaaagaaa aaaatacgga
    20821 aaagtctgcc tatcatgtcg ggaagcgaaa agtatggttc gggcgcggcg gcatgggaaa
    20881 ggatgtttca gcgcgtcagg ctgcttgtcc gaccgtgtca aaaagtcatg ccgtttgcga
    20941 tataataacg cttttcgccg catttgttgg aacaggatat gagtcaaaat acctcggtgg
    21001 gcattgtaac gccccaaaaa attccgtttg aaatgccgct ggttttggaa aacggtaaaa
    21061 ctttgccgcg tttcgatctg atgattgaaa cctacggcga gctgaatgct gaaaaaaaca
    21121 atgcggtttt aatctgccac gcgctgtcgg gcaaccatca cgttgcgggc aggcattcgg
    21181 cggaggataa atatacgggc tggtgggaca atatggtcgg tcccggaaaa ccgattgata
    21241 cggaacgttt tttcgtggtc gggttgaaca atctgggcgg ctgcgacggc agcagcgggc
    21301 ctttgtcgat caatcctgaa acgggcaggg aatacggcgc ggattttccg atggttacgg
    21361 tgaaggactg ggtaaaatca caagccgcgc ttgccgatta tctcggcatc gaacaatggg
    21421 cggcggttgt cggcggcagc ttgggcggca tgcaggcttt gcagtgggcg atttcctatc
    21481 ccgaacgtgt gcgccacgcc ttggtgattg cgtctgcgcc gaaactgtcc acgcaaaata
    21541 tcgcgtttaa tgatgtagca cgtcaggcga ttttgaccga ccccgatttc aatgaaggac
    21601 attaccgcag ccacaacacc gttcccgcgc gcggtttgcg gattgcccgt atgatgggac
    21661 acattacgta tcttgccgaa gacggtttgg gcaaaaaatt cggacgcgat ttgcgttcca
    21721 acggctatca atacggctat agcgttgaat ttgaagtaga atcctatctc cgctatcaag
    21781 gcgacaaatt cgtcgggcgg tttgatgcta atacatattt gctgatgacc aaagctttgg
    21841 actatttcga tccggcggcg gatttcggca acagcctgac ccgcgccgtg caggatgtgc
    21901 aggcaaaatt ctttgtcgcc agcttcagca ccgactggcg tttcgcgccc gaacgttcgc
    21961 acgaactggt caaggcactg attgccgccc aaaaatccgt gcagtatatc gaagtcaagt
    22021 ccgcacacgg gcacgatgcc tttttaatgg aagacgaagc ctatatgcgc gccgtaacgg
    22081 cttatatgaa caatgttgac aaggattgcc gattatgaac ctgcgcgatg atttgcaact
    22141 gatttacgac cggatacccg aaggcagccg cgtcttagac ttgggctgcg gcgacggcga
    22201 attgctggcg gctttggcgg aacacaaaaa atgcagcggc tacggcatcg aaatcgacac
    22261 cgacaacgtg attgctgcga tgtcgcgcgg cgtaaatgtt atccaagccg atttggaaga
    22321 agggttgacc gcattcaacg atcaaagttt tgatgtgatt gttttgagcc aaaccatcca
    22381 agcgatgcag aataccgaaa aaatcctgcg ctgcctgatg cgcctcgcca aacaggcaat
    22441 cgtcagtttc ccgaatttcg gctactggcg caaccgcgtc caaatcgcac tcggcggaca
    22501 tatgccggtt tccgaacgta tgccctacca ttggtacgac acgcccaata tccattggtg
    22561 cacgctcaaa gactttgatt tgttgtgcgc caaaaacaat atccgcgtcc tcgaacgcgc
    22621 cgtgatgacg ggcaacagac aggtcaaaca tttccccaac cttttgggca gccttgcgtt
    22681 ttaccgcgtc gggtaagaca ggttgaaaac cttgtcggca ataacgggtt tctcggttct
    22741 attaacaaca gtccgtctga aaaatgaaac ggcaggtttt cagacggcct tttcaatatt
    22801 ggaaacaata gaatgaaaaa caaaacgtca tcacttccct tatggcttgc cgcaatcatg
    22861 ctggccgcgc gttccccgag caaagaagat aaaacgaaag aaaacggcgc atccgccgct
    22921 tcgtcttccg cgtcccaatc cgatttgcaa ccggccgcat ccgcccctga taacgtcaag
    22981 caggcagaaa gcgcgccact gtgaaattgc accggcctgc accccgccgc cggcattggc
    23041 gatctcatac agcaaatcgc cgaacacatc gactcggact gtctgtttgc cctttcccat
    23101 aacgaactgg aaacccgttt cggcttaccc ggcggcggct atgacaacat acagcggctg
    23161 ctgtttcccg acatccgccc tgaagatccc gactaccatc agaaaatcat acaggcaatt
    23221 gaagacttgc gttacggaac gcgcaccatc agccggcagg cacaagatgc catgatggaa
    23281 caggaacgcc gcctccgaga agcgacgctg ctgctgacac aaggcagtca agaaacccgc
    23341 ggacaaggcg aggaaccgaa acgcgcacgt tattttgaag tttcggcaac atctgcctat
    23401 ttgaaccggc acaacaacgg actcggcggc aatttccaat acatcggcca attgcccggc
    23461 tatctgaaaa tgcacggaga aatgcttgaa aaccaatcac tcttccggct gtccaaccgt
    23521 gaacgcaatc ccgacaaacc gtttttagac atccattttg acgaaaatgg caaaatcacg
    23581 cgtattgtcg tttacgaaaa aaatatctat agtggattaa caaaaatcag gacaaggcga
    23641 cgaagccgaa gacagtacag atagtacggc aaggcgaggc aacgctgtac tggtttaaat
    23701 ttaatccact atacttcaat ccaaacacgg ggcgaatata aaaaaatgcc gtctgaaagc
    23761 ctttcagacg gcatttttca agtaggatat caggcgcgtt tgcggaattc gccggtacgg
    23821 gtatcgattt cgactttgtc gccgttttcg atgtaagaca tcacttggat ttcggtgccg
    23881 ccgaccagac gcgcggtttt catcactttg ccggaagtat cgcctttgac ggcaggctcg
    23941 gtgtagtcga cttcgcgcac gatgatggtg ggcagttcga cagaaatggg attgccttca
    24001 tagaaggtaa cttcgcattg gtcttccata ccgtcaacga tgaatttcaa cgcgtcgccg
    24061 atgttgtcgg cttcgatttc gtattggttg aattcttcgt ccataaagac gtacatcggg
    24121 tcggcaaaat agctgtacgt acagtttttg cgggacagga tgaccacgtc gaatttgtcg
    24181 tcggctttgt aaatggtttc ggaagcagcg ccggtcagca ggtttttcag tttcatgctg
    24241 actttggcgg aagagcggcc gcctttgatg tattcggttt tttgaacgac cataggatcg
    24301 ttgccgacca taaatacatt gccggcgcgc agttcttgtg ctgttttcat tgtgattttc
    24361 caatcaaatc ggttgtcgta aaacgcgcta ttctagcgta ttttttgatg ctttgaaaca
    24421 aaggcggcga gtttttcgga tgcggaaggc tgcccgaaaa gataacggct ccaatcctcc
    24481 gcgccttgcc gccagccgtt ttgatgttgt tgcagggttt gccaacattc gaggcgttgc
    24541 gtggcggata aagcctctcc gccgttgagg tcgtccgaaa ggaggcggtg caccgatgcg
    24601 gtttcgggcg tgtagaagcc gtatgcctta tcccaaaagg cgtggagttt gtcgagatgg
    24661 acgttttcat cttgcgggta gatgtgccaa aagaaaggtt ttccggcaag ctgggtacgc
    24721 acgaaactgt cttcgccgcg tatcacggcg cagtcggcga ggtgcagcaa tttgtcgaag
    24781 tcctgttgcg gcacgaacgg gattttgaca aggcggacgg atgccgtctg aaagacaccg
    24841 ccgtcatttt gcagggcgtt ttgcggaata acgccgcttt gtttgaggct gtcgataatt
    24901 tgcgcccccg ccagcagtag ggtcatcagg ctgcctgcct gttgccacat gtccagccac
    24961 tttgcccaaa catcgccccg atagccgaaa agcagccatt cgggggcgtt tttttcgggc
    25021 agcaccagcc gccggcgcag ggcttcggta tcgaaacgga cggcttcgcg gtaatcgcgt
    25081 tcgcgtatca acccgccgct tttttcgctg aaacccataa accaaaaata tttttgaacg
    25141 ccctcctgcg gcgaaggcat caggtgcagc ctttcattgc tttcctccgc gctcaaatat
    25201 tcccaattca gccaaagcgg tttgtgtcgg cggatgatgt tcagcacatt ttcgggcagg
    25261 tcgcaggcaa aagtttcgat aacggcatcg ggaacgggcg cggtatcaat gtctgccgca
    25321 tcggaatgcc aagtgcggac atgaatatcc tgatgaacga agggaacatc gggcaaatcg
    25381 ggacaaagcg cgcgcaaggc ggacacgtcg tccgtccaca aatgcacctg ccaaccgagt
    25441 tcgcggtgca aaacacgggc gagccgccac gaaacgccga tgtcgccgaa attgtcgatg
    25501 accttgcaaa aaatccaaca ggcaggaaaa gcgtatgtat tcataaccat cgggaaaaca
    25561 agctaaaacg cgccgaaata gtggaacaaa tacacaacca gcaaaacaat cggaataaac
    25621 agaaacatgg tttgacgttt caaatcaaac ccttgtttaa gatgatgctt gatggtttca
    25681 aagaggacga atttaaacaa aaacgtcgct tcgcaggcaa ggatgatgcc gttgatcaga
    25741 tgctggggtt tgtcttcggg cggattggcg gcgaaaacgg caagcgtcgc caaggggatg
    25801 aggcagacgg cgagccagcg gacaagcagg tttcgggaca ttgcaaggct ttcaaaaaag
    25861 caaaacgcca ttatcgcctc aagaacgcac cttgtctaac ggacgcgccg gagatgccgt
    25921 ctgaagcctt gccgccggaa aacgccgcac cgccgaacct ttaaatatac ccgggacggg
    25981 cgttccccga gaaatcggac gctgccgccg atgcctgcgg acaaggcggc atcgggcgcg
    26041 ggcatattga aagcccgacg catcggtttg aacgcatttt ataaaggcgt acggcaaacc
    26101 cgccccttgc cgtcggcgtg cttccgcaaa gcaaaatata agggcggcgc gctttccgct
    26161 ataatccgca tttttccaaa cctgaaacat tatgccgacc ccgaaagcct ttaccctgct
    26221 cgggccgacc gcctgcggca aaaccgcgct cgccctcaaa attgccgaaa cccttccggt
    26281 cgaaatcatc agcctcgatt ctgcactgct ctataccggt atggacatcg gcacggcgaa
    26341 accgtccgcc tccgaacgcg cctttgtccc gcaccacctt atcgacatca ttacgcccgt
    26401 tcagacatac agtgccgccc gttttgtcga agactgcacg cgtctgaccg gcgaaatcac
    26461 ggcgcgtggg aaatgtccgc tgattgtcgg cggcacaatg atgtatttcc gcgccctgac
    26521 ccaaggtttg aacgatttgc ccgaagccga tgcctgcctg cgtgccgact tggacgaaca
    26581 aaaacagatg tacggtttgg attttctcta ccgcaccctg caaaaagtcg accctgaaac
    26641 agcatgccgt ctgaaaccga acgacagcca gcgcatcgga cgcgcccttg aagtctatta
    26701 cctgaccggc aggccgatga gcgcgcacct gaacggacag cccgaacaca ccctgccctt
    26761 cgaactctac accgccgccc tgattcccga aaaccgcgcc cgactgcacg aaaacatcgc
    26821 cctgcgtttc cacctgatgc ttgaacaggg ctttatcggc gaagtagaaa acctgcgccg
    26881 ccgctatccc ggcctgaccg ccgactcccc cgccatccgc tgcgtcggct accgtcaggc
    26941 gtgggaacac ctcgacggtg caaccgacag acaaaccttc atcgaaaaag gcattgccgc
    27001 cacgcgccaa cttgccaaac gccaactgac ttggttacgc aaaacacctt tagactgcgt
    27061 tgccgacccg ttttcagacg gcacttcagg cacgcgcctg attgaggcgg caaaacggtt
    27121 tttcggcgaa taaaccatgc tggccacgcc gccgctcgcc cccaaaaccg tcgccgccct
    27181 gcacaggctc ggtatccgta cgctcgaaga actgcggcaa aacggttcgg tcaaagcctt
    27241 tctgctgctc aaagcatcgg gtttgaccct cacgaaaagc acgctgtggc aactcgagtc
    27301 gctgctgaac ggcacgccgc cgcaagaaat gtcccaagcg cataaagact gcctgcttgc
    27361 cgagttgaaa aaccatccgc ccgtcgccgc ctttccgccg caggaagaaa tgggatactt
    27421 catgcgcgaa gcactgcgcc aagcagaaca atccgccgcc gacggcgaaa tacccgtcgg
    27481 ggcagtcatc gtttcagacg gcaaaatcat cacatcggca cacaacacct gcattgccga
    27541 ctgcaatgtc agccgacacg ccgaaatcaa cgccttggca caggcaggca gcgaaatgca
    27601 aaactaccgc cttgacggat gcgacatcta catcaccctc gaaccctgcg ccatgtgcgc
    27661 gtccgcactg atacaggcac gaatcaggcg cgtgatctac ggtgcggcag aacccaaaac
    27721 cggcgcggca ggcagcatcg tcaacctgtt tgccgacaaa cgcctcaata cgcacaccgc
    27781 catacggggc ggaatcctgc aagaagaatg ccgcgccgta ttaagccgtt ttttccaaaa
    27841 caaaagaaaa ggttgaaccc atgaccgccc tactcgtcat cctcgccctc gccctgatag
    27901 ccgtcggcac ggcaggcatc gtctatcccg ccctgcccgg cttggcattg atgtttgccg
    27961 gaacatggct gcttgcctat gccggcggct atcaaatcta cggcgcaggc atcttgtgga
    28021 cggtcggact catcagcctt ggcggcatac tggcggacta tatggcaggc atgttggggg
    28081 taaaatacac tggggcaggc aaactcgccg tccgaggtgc attggccggc agcatcatcg
    28141 gcatattttt ctccctgccc gggctgatac tcggcccctt tatcggcgcg gcggcaggcg
    28201 aactgatcga tcggcgcaat atgcttcagg caggtaaagc gggcttgggt acgctgttgg
    28261 ggcttgtcgt cggcacggcg ttcaaaatcg gctgcgccgt atccatcttg cttatcctgt
    28321 tggtgaaata catcgcctac ctgttttaac cccttcagac ggcataccgg caaattgcta
    28381 taatgccgtc tgaaatttcc gacaaagcac cattatggca aaccaaagac ccatctacgg
    28441 cttccacgcc gtcaacgccc gattgtggca aaaccccaaa tcgattaccg aactctatat
    28501 ccaagaaggc aaatccgacg cacgcacgcg cgaagtgctg gaaaaggcgg caaacgaaaa
    28561 catccgcgta tattttgccg atgccgaccg cctcaacgcc atcagcaaag gcgcgcgcca
    28621 tcagggcgtg gtcggattta tcgatgcctc caaaaaccac gtccacctcg aagacgtatt
    28681 ggaaaacctc agcgaaccgc cgctgctgct gatactcgac ggcatcaccg atccgcacaa
    28741 cctcggcgcg tgcctgcgta ccgccgacgc aatgggcgta cacgccgtca tcgcgccgaa
    28801 agacaaaagc gcgggactga acgccaccgt cagcaaagtc gcctgcggcg cggcggaaac
    28861 cgtcccctac atcaccgtaa ccaacctcgc ccgcaccctg cgcgaattga aagaatacgg
    28921 catctggatt atcggcaccg acataggcgg caacgccgac ctttaccatt gcaatctgcc
    28981 cgacagcgcg gcatgggtga tgggcaacga aggcgacggt atgcgccgcc tcacgcgcga
    29041 acattgcgac atgctggtgt ccatacccat gttcggcacg gtcgaaagca tgaacgtctc
    29101 cgtgtccgca ggaatggtat taagcgaaac ccgccggcaa cgcgtattga aaaacaaaaa
    29161 agcgtaagcc tcaaaaacaa tgccgtctga aacagggatt ccgtttcaga cggcattcgt
    29221 tttcaaaatc agtgttccaa atggtcggta tcaaacttga ccgccgcctc ggttttatct
    29281 tccggcaaga ccaaattcag caagactgcc gtaatgccgc cgccggaaat agagttttgg
    29341 aacaagacgg gcaggttttt aaacacttcc ggctcaaacc ccacgcccaa gcccaaaccg
    29401 accgacgttg ccgcaatcac cgcctcgcgc ctgcggatgc cgtggccgac caaaatccgc
    29461 acgcccgcaa tcgcaatcag gccgaacatc aaaaccatcg cgccgcccaa caccggactc
    29521 ggaatcgtcg taaacgcgcg tccgacaacg gggaacagac ccaacagcac caaaatcacg
    29581 gcaatatatt tgcccacgtg gcgcgaagcc actccggtca tctgaatcac gccgttgttt
    29641 tgcgcaaacg tcgtcagcgg cagcgaaccc aaagccgtcg caatcaccga taccaagccg
    29701 tcagccaaca cgccgccgcg caggcgtttg gtgtattctt cgccttcaat cggctggtcg
    29761 gacaccattg ccgttgccgt caaatcgccg accgcctcaa acacgcccaa caagaaaatc
    29821 gcacccgccg caataaatgc gtgccagtca aaagcaaaac catatttaaa cggtacgggc
    29881 agcgtaaccg gcggcaggtt ttgcagtgcg gaaaaatcca ccttgcccaa aaacagcgcg
    29941 acaatgtagc cggcaatcag accgaccgca atgccgctca tccgcagcag cggatttttc
    30001 atgcagttaa atatcaacac aatcagcagt accagcgaag ccagccccaa gttttccatc
    30061 gagccgaacg tgccgtccgc cttcgcgccg aagccgccgc cgaaatcggc aataccgacg
    30121 tgtaccaaac tcaaaccaat gagcatcacg accacgccgc taaccgtcgg cgtaatcact
    30181 tttttcaaat acggcagcag ccacgccgag aaacacacca aaaacgcacc ggcaaacgat
    30241 acgcccaaga gcgtcgaaac catcgccccc tcactcaaac cgccctcttt catccccgcg
    30301 ccgagcgcaa tcatgacggt aacgctggat ggacagcatc cccgagccga ccggcccgaa
    30361 gcggttgacc tgcaaataag tgccgacacc cgacgcaacc atcgccatcg acaccagata
    30421 cgccgtcatc tccaccggca attccagcgc gccgcccaca atcagcgcgg gcgtaatcat
    30481 cggcacgaaa atcgccaaaa gatgggtaac cgcgctcaag agcgcattac cgaacggcgg
    30541 cctgtcttcc aaaccgtaca ccaaatcagg cgaatccgcc tgttttttca ttgtttcagc
    30601 cattggagtc tttctatccg ttgccaaacg ttaaaaaatt ttaagaacga aattgtaacg
    30661 aatcaaagat tccttatcaa ttcaaatcct catgtgcaaa aacaccaccg gttgtccgaa
    30721 ttcaaatttc tttgaatttc cgcaccggac agtccttcat ttccagagag ccttcatgcg
    30781 cttaacacat tttaatcctc aacggcgcat ccactgctta aacccaataa tttcatgatt
    30841 ataaaaaaaa tattatttat taagatatga tgggacttaa gccttggaac caaccgtgcg
    30901 gccgatatgt ttcacacaaa caacaaagta agctacaatc cgaagcataa tcgacctcac
    30961 tcaatgaata aaaggtttcc gtatgttaca aggtagcttg gttgccctga ttaccccgat
    31021 gaatcaagac ggcagcatcc attacgacca actccgccaa ttaatcgact ggcacatcga
    31081 aaacggcacg gacggcatcg tcgctgccgg cacgacaggc gaatccgcca ccctctccgt
    31141 cgaagaacat ttaagcgtaa tcgaagaaac ggtcaaacac gttgccaaac gcgtccccgt
    31201 catcgccggc acaggggcga acaacaccgt cgaagccatc gccctttccc aagccgccga
    31261 aaaagccggc gcggactaca ccctctccgt cgtcccctat tacaacaaac cttcccaaga
    31321 aggcatgtac cgccatttca aagccgttgc cgaagccgcc gcggttccga tgatactcta
    31381 caacgtgccc ggcagaaccg tcgtcagcat gaacaacgaa accatcctgc gccttgccga
    31441 aatccccaac atcgtcggcg ttaaagaagc aagcggcaac gtcggcagca acatcgaact
    31501 catcaaccgc gcgcccgaag gcttcgtcgt cctttccggc gacgaccaca ccgccctgcc
    31561 gttcatgctc tgcggcggac acggcgttat taccgtagcc gccaacgccg cgcccaagct
    31621 ctttgccgat atgtgccgtg ccgcgctgca aggcgacatt gcccttgcac gcgaactcaa
    31681 cgaccggctg attccgattt acgacaccat gttctgcgaa cccagtcccg ccgcaccgaa
    31741 atgggcggta tccgccttgg gcagatgcgg accgcacgtc cgcctgccgc tcgttccgct
    31801 gacggaaggc ggacaggcaa aagtccgcgc cgccctgaaa gcctcaggac aactttaaat
    31861 atagccatgc cgtccgaacc gttcggacgg cataacatga caaacacttt aatatccatc
    31921 acacaggatg acacgatgac ccatatcaaa cccgtcattg ccgcgctcgc actcatcggg
    31981 cttgccgcct gctccggcag caaaaccgaa cagcccaagc tcgactacca aagccggtcg
    32041 caccgcctga tcaaactcga agtcccgcct gatttgaaca accccgacca aggcaacctc
    32101 taccgcctgc ctgccggttc gggagccgtc cgcgccagcg atttggaaaa acgccgcaca
    32161 cccgccgtcc aacagccagc cgatgccgaa gtattgaaaa gcgtcaaagg cgtccgcctc
    32221 gagcgcgacg gcagccaacg ctggcttgtc gttgacggca aatcccccgc cgaaatctgg
    32281 ccgcttctga aagccttttg gcaggaaaac ggcttcgaca tcgaatccga agaacccgcc
    32341 atcggacaaa tggaaaccga gtgggcggaa aaccgtgcca aaatccccca agacagcttg
    32401 cgccgcctat tcgacacagt cggtttgggc ggcatctact ccaccggcga gcgcgacaaa
    32461 ttcatcgtcc gtatcgaaca gggcaaaaac ggcgtttccg acatcttctt cgcccacaaa
    32521 gcgatgaaag aagtgtatgg cgacaaaaac aaagacacga ccatgtggca gccttccgct
    32581 tccgacccca accttgaggc cgctttcctg acgcgcttta tgcaatattt gggcgttgac
    32641 ggacggcagg cggaaaacgc attggcaaaa aaaccgaccc ttcccgccgc caacgaaatg
    32701 gcgcgtatcg aaggcaaaag cctgattgtc tttggcgact acggcagaaa ctggcggcgc
    32761 accggccttg ccctcgaccg catcggactg accgtcgtcg gtcaaaacac cgaacgccgc
    32821 gccttcctgg ttcaaaaagc cccgaacgaa agcaatgcag ttaccgaaca aaaaccgggg
    32881 ctgttcaaac gcctactggg caaaggcaaa gcggagaaac ctgccgaaca gccggaactg
    32941 attgtctatg ccgagcctgt cgccgacggt tcgcgcatcg tcctgctcaa caaagacggc
    33001 agcgcatatg ccggcaaaga cgcatccgca ctgttaggca aactccattc cgaactgcgt
    33061 taaaccgttc aaaacaaaag cagcttccaa atagaagctg cttttttgtg cggaactttt
    33121 tacaacaaac ggggcaaaat atcctcaacc gcctgaacca acgcatccgc caaaggcgga
    33181 tcgaacgcac aatgccccgc ctggaccacc ctcaattccg cttcgggaaa ggctttcgac
    33241 agctcccacg cactctgcat aggcgtacac aaatcatacc gcccctgcac gataacggtc
    33301 gggatatgcc gtattttgcc gatattgttc aaaatcgcct tatcaccctg caaccaaccg
    33361 ccgttgacaa aataatggtt ttccaaacgc gcgattgcca gtgaggcata agcatcttca
    33421 tccactccct ccggctcgaa acgaatcaga tagctttccc aatccgccca agccttcgcg
    33481 gcagacaggc acacttcttc atcttgatga aacagcaacc cgtgatacgc ctcaatcagc
    33541 cggttccgcc gattttcagc aatcggcgcg acaaattttt gccattgttc cggataaatc
    33601 cggctcacgc cgcccgcctc gttcagccac gccgtttcag acggcctgca caaaaatatc
    33661 ccgcgcaaca ccaatccctt tacccgttcg ggatgggttt gggcataagc cagcgacaaa
    33721 gtgctgcccc acgaaccgcc gaacaccagc catttcccga tacccagcat ttcacggact
    33781 ttttcaatat ccgccaccaa atcccaagtc gtattgtctt ccgcacaagc atacgggtgc
    33841 gaacgtccgc aaccgcgctg gtcgatgatg acgatgcgga acacatcagg atgaaaaaaa
    33901 ccccgacatt caggcgaagc ccccgcgccc ggcccgccgt gtaaaaaaat gaccggcaca
    33961 ccgtcgggat tgcccgactc ttcccaataa atttgatgaa tttcagacac ctgcaagtat
    34021 ccgctatgaa aaggctgttt tatttcatac atcatcttct cccatattgc ttctgacaat
    34081 ccgaacatcc cgacacgcga catcccgcac gaaacacaag ccgccccgaa aggcaaatgc
    34141 cgaaaaatcg cgataaaaac catcaatcgg aaaatattgc ataaacaggc gaaaatatat
    34201 cattgaaaag aaaacccata caatgtctga cagaaacaag ccgctttgcc attgtttcct
    34261 aacagttaaa ccccgccctt caaggcggcg gggcagggct tgattcaaaa tggcgcaagc
    34321 ccctgccctc aaatccaaca cgcaggatta aaccataata gcggctttct tattatttct
    34381 tattgaaaca ccgcccggaa cccgatataa tccgcccttc aacatcagtg aaaatctttt
    34441 ttttaaccgg tcaaaccg
//
LOCUS       DAXDXW010000016        30356 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXDXW010000016 DAXDXW010000000
VERSION     DAXDXW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30356
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.016"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..173
                     /locus_tag="ACFB64_000643"
     CDS             <1..173
                     /locus_tag="ACFB64_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HFC1209057.1"
                     /translation="EPLPPSALSESEANRDLGWMLHDIDFDHGNTPHFFRTQMKDGLI
                     DVPPFYAEEVKA"
     gene            170..1456
                     /gene="cas8c"
                     /locus_tag="ACFB64_000644"
                     /pseudo
     CDS             170..1456
                     /gene="cas8c"
                     /locus_tag="ACFB64_000644"
                     /inference="COORDINATES: protein motif:HMM:NF021243.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
     gene            1476..2236
                     /gene="cas7c"
                     /locus_tag="ACFB64_000645"
                     /pseudo
     CDS             1476..2236
                     /gene="cas7c"
                     /locus_tag="ACFB64_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777774.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
     gene            2263..2631
                     /locus_tag="ACFB64_000646"
     CDS             2263..2631
                     /locus_tag="ACFB64_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209058.1"
                     /translation="MKYWLVGASWGGQGHQDQFFVENGYWVLEWESSQQPDQFAKGEK
                     IQVGDRIAIKRMKGQGSSEIKILHIGIVKGVISETNKIICVVDWIVKNLDRNVESRGC
                     FKSIHDPYDKDEWIEKIFCL"
     gene            2638..3288
                     /gene="cas4"
                     /locus_tag="ACFB64_000647"
     CDS             2638..3288
                     /gene="cas4"
                     /locus_tag="ACFB64_000647"
                     /EC_number="3.1.12.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HFC1209059.1"
                     /translation="MTALLTETQRENQDTRLIPLSALQHYAFCPRQCALIHNAQAWAE
                     NCLTAQGKALHERVDSDEPETHKGVRFERTVHVSAEKPGISGILDLVEADTKTGRLKP
                     VEYKRGKPKPDPGDEIQLCAQGLCLEEMTGQTVSEGALWYMQTRHRVPVVFSDGLRAQ
                     TLATIAAVCKLLNSGQTPPPDYGKRCKACSLAEICQPELLGKRDRSVGYVKGLFGE"
     gene            3404..3763
                     /locus_tag="ACFB64_000648"
     CDS             3404..3763
                     /locus_tag="ACFB64_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209060.1"
                     /translation="MTRNIEHQFSNLSDVGEKLELENPTVENVVDILVDIGHDDRVYT
                     FHDDFLGLKSGLPQDLLSKHIDELEEGDFADRYSDEIDKILDNANIIFYHLERELSED
                     DLEEIREERERLGLEDD"
     gene            3906..4529
                     /locus_tag="ACFB64_000649"
     CDS             3906..4529
                     /locus_tag="ACFB64_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209061.1"
                     /translation="MTQKNDFKNANFYGNTQIVNGDVHNVHTQNTPNSINENEIATYT
                     PEPIWRSPITMAWLTWASFLISLAGLLPIYNCIVKPILYLVNGKTMIQSEINNTYIFI
                     LIAIAVLFLITITLRRITKYQTRYPLVFNYAISGMGHRLSLEKISISQCPQCGGEMKY
                     YNKPIEWENIRYSDGSKKRRVTRTTPALECKRNREHWYKVDPAEDKI"
     gene            4589..4945
                     /gene="cas1"
                     /locus_tag="ACFB64_000650"
                     /pseudo
     CDS             4589..4945
                     /gene="cas1"
                     /locus_tag="ACFB64_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
     gene            4906..5226
                     /locus_tag="ACFB64_000651"
                     /pseudo
     CDS             4906..5226
                     /locus_tag="ACFB64_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
     gene            5404..5589
                     /locus_tag="ACFB64_000652"
                     /pseudo
     CDS             5404..5589
                     /locus_tag="ACFB64_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
     gene            complement(5629..7557)
                     /gene="rpoD"
                     /locus_tag="ACFB64_000653"
     CDS             complement(5629..7557)
                     /gene="rpoD"
                     /locus_tag="ACFB64_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC1209062.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKICKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEVSAANLAELKQKVIGHFAQIEKDYKKMIGCLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLQWIEEEIAKGRVWSNALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            complement(7744..9516)
                     /gene="dnaG"
                     /locus_tag="ACFB64_000654"
     CDS             complement(7744..9516)
                     /gene="dnaG"
                     /locus_tag="ACFB64_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC1209063.1"
                     /translation="MIPSDFIDELLAKTDIVGIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAVAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRHYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLVQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETIDRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            complement(9661..12411)
                     /gene="secA"
                     /locus_tag="ACFB64_000655"
     CDS             complement(9661..12411)
                     /gene="secA"
                     /locus_tag="ACFB64_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC1209064.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRVLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSETGHEH
                     AEQILTQMGLLAENDSLYSAANISLMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSRLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDIGDLMQEIR
                     SDAVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLQEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQAIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAAVEEQPVGNIQSIHSES
                     PDIEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            12543..12965
                     /locus_tag="ACFB64_000656"
     CDS             12543..12965
                     /locus_tag="ACFB64_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC1209065.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFDSAAAKLEERHPELAEALEELVRKYGA"
     gene            13248..13799
                     /gene="azu"
                     /locus_tag="ACFB64_000657"
     CDS             13248..13799
                     /gene="azu"
                     /locus_tag="ACFB64_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC1209066.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAGEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKVTLVD"
     gene            13894..14403
                     /locus_tag="ACFB64_000658"
     CDS             13894..14403
                     /locus_tag="ACFB64_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC1209067.1"
                     /translation="MNPFETQSVTFAEPIEMLYACHGKVRRFCGQIAMLSGYIAENGC
                     NQLVLQTIRQISRYFNVAAPLHHEDEEENFFPLLLQYAPQAREGVDELLRQHIGLYDN
                     WAAVSAEFAKLEADNAYIPDAEAFKRFVEGYDVHLAIEEPLFDMGKTFIPEEKLTEIG
                     EIMAARRCK"
     gene            14437..15057
                     /locus_tag="ACFB64_000659"
     CDS             14437..15057
                     /locus_tag="ACFB64_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI_0552 family protein"
                     /protein_id="HFC1209068.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAADLSEPFAPPHIERWCNGWQVRAHFFAYFKYAQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTEKYAAFDMWHGAESEYDDYRTVAQQSESDR
                     RLQNDEDFFCIGKHIERDDLGKQDVVKWIAETVEDLLPLYEACHGK"
     gene            15170..16315
                     /gene="dapE"
                     /locus_tag="ACFB64_000660"
     CDS             15170..16315
                     /gene="dapE"
                     /locus_tag="ACFB64_000660"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC1209069.1"
                     /translation="MTETQSLELAKALISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAVNPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTETGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARTAIAETCGVEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYERILARLLAEKAV"
     gene            16735..17550
                     /locus_tag="ACFB64_000661"
     CDS             16735..17550
                     /locus_tag="ACFB64_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1209070.1"
                     /translation="MGALGGIVQHHTALYRSVAVYEPTVVIVRRGCKKLRWAGRELRI
                     AAGEAVGLAGGQTLDVINIPDSDGLYQAQWIAFEQETVERFAAQYGTAQAVCDAVKLP
                     HPGRMGAAFDYAAAVLADEEVPHNAAEAALCGVLAWLQHDGIGFAVYGGVNLMRQIRK
                     LITADMAADWSSAMLAQRLNCSEAALRRRLARQDTNFRTLLTDVRMMRALTLLQVTQW
                     SVAQIAGAVGYDCPSRFSARFKELFGCVPSVVRSEAEPAAYRRTGQVSVGVRP"
     gene            complement(17528..18085)
                     /locus_tag="ACFB64_000662"
                     /pseudo
     CDS             complement(17528..18085)
                     /locus_tag="ACFB64_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273197.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            18435..19187
                     /locus_tag="ACFB64_000663"
     CDS             18435..19187
                     /locus_tag="ACFB64_000663"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC1209071.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPYPNLTECEAGQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLELRYPDSLNELVCAAIATPLGEMLAVFGVKGLC
                     LLEFVGQKYLEQEIAAVQKALRGRFVFREDGRMQFLRQELDLYFQCRLKTFATPLEMI
                     GTAFQKQAWDVLLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSVMIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            19378..19453
                     /locus_tag="ACFB64_000664"
     tRNA            19378..19453
                     /locus_tag="ACFB64_000664"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19411..19413,aa:Asn,seq:gtt)
     gene            19584..20594
                     /gene="waaF"
                     /locus_tag="ACFB64_000665"
     CDS             19584..20594
                     /gene="waaF"
                     /locus_tag="ACFB64_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC1209072.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKKLHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRELGRRGYDRVIVLPGSLKSAVIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHQSQEDFDGHSRFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPARHFAELGKHYSEAGWQVWLFGS
                     QKDNEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            20649..21107
                     /gene="smpB"
                     /locus_tag="ACFB64_000666"
     CDS             20649..21107
                     /gene="smpB"
                     /locus_tag="ACFB64_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC1209073.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHITTHIKPDAVRPRKLLLKQSEINKLIGKTERAGY
                     TIVPLDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            22165..23508
                     /locus_tag="ACFB64_000667"
     CDS             22165..23508
                     /locus_tag="ACFB64_000667"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC1209074.1"
                     /translation="MGALELTNSLWEPRWNSNIDYLITKNAEIRFNTKNESLLVKEDY
                     AGGARFRFAYDLKDKVPEIPVLTFEKNITGTSDIIFEGKALDNLKHLDGHQIVKVNDT
                     ADKDAFRLSSKYRKGIYTLSLQQRPEGFFTKVQERDDIAIYAQQAQAANTLFALRLND
                     KNSDIFDRTLPRKGLWLRVIDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQL
                     SIGLMGGQAEQRSTFRNPDTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYVDSWMQYQ
                     RFRHRINTEYATERFTSKGITASIEAGYNALLAEHFTKKGNSLRVYLQPQAQLTYLGV
                     NGKFSDSENAQVNLLGSRQLQSRVGVQAKAQFAFTNGVTFQPFVAVNSIYQQKPFGVE
                     IDGDRRVINNKTVIETQLGVAAKIKSHLTLQASFNRQTSKHHHAKQGALNLQWTF"
     gene            complement(23491..23796)
                     /locus_tag="ACFB64_000668"
     CDS             complement(23491..23796)
                     /locus_tag="ACFB64_000668"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209075.1"
                     /translation="MDPIVGLARARGRGELDRIEQMDLDFFHRTRARYLELVKDNPKA
                     VVINAEQTIELVQAEIESAVKIGGNYTKNDRTFKIIKSAVNFPADFILLQFNLKRPL"
     gene            complement(23837..25204)
                     /locus_tag="ACFB64_000669"
     CDS             complement(23837..25204)
                     /locus_tag="ACFB64_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC1209076.1"
                     /translation="MPDLHTEFSRLLPADEIAEPSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFPPEILSNEVYRYEN
                     DINSTVYRNVLARNGTIAAEHGIGIIKKQWLPAVRTPSEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(25314..25670)
                     /locus_tag="ACFB64_000670"
     CDS             complement(25314..25670)
                     /locus_tag="ACFB64_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209077.1"
                     /translation="MKKSLFAAALLSLALAACGGEKAAEAPAAEASSTEAPAAEAPAA
                     EAPAAEAPAAEAPAAEAPAAEAPAAEAAATEAPAAEAAATEAPAAEAAATEAPAAEAP
                     AAEAPAAEAPAAEAAK"
     gene            26143..26625
                     /locus_tag="ACFB64_000671"
     CDS             26143..26625
                     /locus_tag="ACFB64_000671"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC1209078.1"
                     /translation="MAIAKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSSDDAFGEQDSELVRIEDAGAFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGSHGHYHH
                     "
     gene            26712..27524
                     /gene="hpnC"
                     /locus_tag="ACFB64_000672"
     CDS             26712..27524
                     /gene="hpnC"
                     /locus_tag="ACFB64_000672"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC1209079.1"
                     /translation="MSVGHYENFPVGSLVLPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLSGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGDLTDYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(27597..28067)
                     /locus_tag="ACFB64_000673"
     CDS             complement(27597..28067)
                     /locus_tag="ACFB64_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC1209080.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLGALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     PEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            28158..29999
                     /locus_tag="ACFB64_000674"
     CDS             28158..29999
                     /locus_tag="ACFB64_000674"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC1209081.1"
                     /translation="MRTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELTVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVHHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDGRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPAFMLLVNGRRQKRRPKA
                     VAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKFSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAVYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRADGSRSEPLLGFVKADKFIEWYEQNR"
BASE COUNT         7240 a         7040 c         8339 g         7737 t
ORIGIN      
        1 gtgaaccgct gccgccgtcg gcgttatcgg aaagcgaggc aaaccgcgat ttgggctgga
       61 tgctgcacga tattgatttt gatcacggca acacgccgca ttttttccgc acgcaaatga
      121 aagacggctt gattgatgtg ccgccgtttt acgccgagga ggtgaaagca tgattcttgc
      181 gtcccttgtc cgctattacc gccgtttggc aacggaaacc gatgaaacgg gcaacccgaa
      241 agtgccgtct tatggtttta gcgaggagaa aatcggctgg attttggtgt tggataaaga
      301 aggtcgtctg aaaaccgttg tgccgaatct gactgccgat aaaaagccgc agccgaagct
      361 gatgagtgtg ccgcgccctg aaaaacgcac gtcgggtatc aaaccgaatt ttttgtggga
      421 taaaaccgcc tacgcgcttg gcgtggaagc caataaaaac aaagccgaag ccaaagaaaa
      481 accgtttacg ccgtctgaaa aaacctttga agccttcaag caataccatc tcgatttact
      541 gcaaaacagc gaagacgaag gtttacaagc cttatgccgt tttctgcaaa actggcagcc
      601 tgcacatttc gctgccgaaa acctgcctgc cgaaatgctt gattccaaca ccgcattttc
      661 tcttgaaaaa ccgaccgctc ttatccataa acgcgaagcc gcgcaaacct tgtgggcagg
      721 ctgcctgaaa agtgatgaag cactcgaaag cttatgcctg attagcggcg acaccgcgcc
      781 gattgcacgg ctgcatccgg cgattaaagg cgtgtttggc gggcaaagct ccggcggttc
      841 gattatttcg tttaataaag aagccttttc ctctttcgga aaagagcaag gcgcaaatgc
      901 gcctgtttcc gaacaatccg cctttgccta caccaccgcg ctgaactatc tcttgcgccg
      961 cgaaaataat cactgcctga ccataggcga tgccagcacg gtcttttggg cggaagcgga
     1021 tgatatagtg gattaaattt aaatcaggac aaggcggcga aaccgcagac agtacaaata
     1081 gtacggcaag gcgaggcaac gccgtactgg tttaaattta atccactata acgccacggc
     1141 gcaggctgcc gaaggcttct tcgcgcaagt gtttacgccg tcggatgacg aacaagaaag
     1201 cgccaaaatt ttcaacgtat tggaacaaat cggcaaaggt cgtccgctgc aagaaattgc
     1261 gcctgaactc tctcccaata cccgttttta catcttgggg cttgccccca atgctgcgcg
     1321 gatttctgtt cggttttggc tggacaccac atttgggcag ctggcggaaa acttggcgca
     1381 tcattggcaa gatttagccc ttgagccttg tgcgtggaaa acgccgccgt ctatttggcg
     1441 gctcttgttg caaactgccg tgctgggcaa aagcgaaaac atccaaaggc ttggtaaccg
     1501 atgtctgcct gaaacgcaaa atccgtaatt ttgtcgaaat cagcagcgaa aacgaagccg
     1561 gttacgaaat ctacgtcaaa gaaaaaagcg tgctgaacct gcaaaacaaa cgcgcttatg
     1621 aagcactcgg cattgagtct gaagccaaaa actgccgaag gacgaagcca aagcccgtga
     1681 cattaccgcg tggatgtgta aaaacttctt cgatatccga accttcggcg ctgtgatgac
     1741 caccgaagtc aacagcggac aagtgcgcgg cccggtacaa ctggcgtttg cccaatccat
     1801 cgacccgatt gtgccgccgg aagtttccat cacacgcatg gcggtaacca acgaaaaaga
     1861 cttggaaaaa gaacgcacca tggggcgcaa atacatcgtc ccttacgtgg tctaccgcgt
     1921 gcatggcttt atctccgcca accttgccgc caaaaccggt ttttcagacg acgacttagc
     1981 caaactctgg caagccctga cgttgatgtt tgaacacgac cgctcagccg cccgtggcga
     2041 aatggcggca cgcaaactgg ttgttttcaa acacgacagc gcactcggca gccagcctgc
     2101 acataaactg tttgacaccg taaaagtcga acgcgtaaac ggcgaatcag gtacgcccgc
     2161 aagcggtttt ggcgattaca aaatcagcgt ggtttcagac gggctaaatg gcgtgagtgt
     2221 ggaagagtat ttgtaagttt tcaataaatc aggagtaacg atatgaaata ttggctagtt
     2281 ggagcctctt ggggagggca ggggcatcaa gatcaatttt ttgttgaaaa tggttactgg
     2341 gttttggagt gggaatcctc tcaacaacca gatcaatttg caaaaggaga aaaaatccaa
     2401 gtaggtgatc gaattgcaat caaacgcatg aaaggtcaag gatcgtctga aattaagatt
     2461 ttacatattg gaatcgttaa gggggttatt tctgaaacga acaaaattat ctgtgtcgtt
     2521 gattggatag ttaaaaattt agatagaaat gttgaaagta gaggatgctt taaaagtatt
     2581 catgatccat atgataaaga tgaatggata gaaaaaattt tttgtttgta gcaaaagatg
     2641 accgcacttt taaccgaaac ccaaagggaa aatcaggata cgcgcctgat tcccctttcc
     2701 gccctgcaac actacgcctt ctgcccgcgt caatgtgctt tgattcacaa cgcgcaggcg
     2761 tgggcggaga actgtttgac cgcgcagggc aaggcactgc atgagcgggt ggattcggac
     2821 gagcccgaaa cgcacaaggg cgtgcgtttt gagcggacgg tacacgtttc ggcggaaaaa
     2881 ccgggcatca gcggcatatt ggatttggtg gaagcggata caaaaacagg ccgtctgaaa
     2941 cctgtggaat acaaacgagg caagcccaaa cctgacccgg gggatgaaat ccagctttgc
     3001 gcccaaggtt tgtgtttgga agaaatgacg gggcaaaccg tttcagaggg cgcgctgtgg
     3061 tatatgcaaa cccgccaccg tgtccccgtc gtgttttcag acggtctgcg cgcccaaacc
     3121 ctcgccacca tcgccgctgt gtgcaaactc ctaaacagcg gccaaacccc gccgcccgac
     3181 tacggcaaac gctgcaaagc ctgctcgctg gcggagattt gccagccgga gttgctggga
     3241 aaacgggata ggagtgtggg atatgtgaag gggttgtttg gggagtaata ttttatttga
     3301 aaaaaattat tacgttaatt aacttgaatc ttccaaatta atcgcaattt ctgaaaacga
     3361 gcaatcatgc tcaattgtaa ttacacattt taaggagtcg taaatgacaa gaaatataga
     3421 acatcaattt agcaatctat ctgatgttgg tgaaaaacta gagttagaaa atcctactgt
     3481 agagaatgtc gtagatattt tagtggatat tggacatgat gaccgagttt atacttttca
     3541 tgatgacttt ctcgggttaa aaagcggttt gccacaagac ttattgtcga aacatatcga
     3601 cgagcttgaa gaaggtgatt ttgcagatag atattcagat gaaattgata aaatactgga
     3661 taatgcaaat ataatattct atcaccttga acgagagctt agtgaagatg atttagagga
     3721 aatacgagaa gaaagagaaa gactgggttt agaagacgat tagtatactt gttgtcatag
     3781 aatagattca atacatacag gttgggttat gctgttttac ccaaccagct aacctcaggg
     3841 catatttaat attatttaat tctgaataat attctattga agtttcaaga tatctaagga
     3901 aaaacatgac acaaaaaaat gattttaaaa atgctaattt ttatgggaat actcaaatag
     3961 ttaatggtga tgttcataat gtacatactc aaaatacacc aaattcaata aatgaaaacg
     4021 aaatagccac atacaccccg gaaccaattt ggagaagccc cattacgatg gcttggctga
     4081 catgggctag ttttctgatt tctttagcag ggctattacc tatttataat tgtatagtta
     4141 aacccatatt atatttagta aatggtaaaa ctatgataca aagtgaaata aataatactt
     4201 atatatttat tttaatagct attgctgtat tatttcttat cactattaca ttacgcagaa
     4261 ttactaagta tcaaactaga tacccattag tttttaatta tgctattagc ggtatggggc
     4321 atcgtttaag tctagagaaa attagtattt ctcaatgtcc acagtgcggt ggagaaatga
     4381 aatattataa taagccaatc gagtgggaga atatacgtta ttctgatggt agcaagaaaa
     4441 gaagggtaac aagaactaca ccagcattag agtgtaaaag aaatcgtgag cattggtata
     4501 aagttgatcc ggcagaagat aaaatctaat ttttagctag cccctcgttt aaccccaaaa
     4561 gcacggtcaa aacctaagga gttttcccat gcgcaaactg caaaacacac tctacatcac
     4621 cacccaaggc agctatctgc ataaggagcg ggagacactg gtggtggagc aggagcgtaa
     4681 gaaggtggcg cagttaccgg tgcattccat cgggcatatt ttctgttttg ggaatgtgct
     4741 tgtgtcgccg tttctgttgg ggttttgcgg cgaaaataat gtgaatttgg cgttttttac
     4801 cgaaaacggg tgttatttgg gacggcttca ggggcggcag aacggcaatg tgctgttgcg
     4861 tcgggcgcag tatcgggtgt cggagcaaag tcccatgccg attgcgtgca atatcattgc
     4921 ggcaaagatt cgggcgatcc ggaaacgtgc aagggcgtgc gttttgagcg gacggtacac
     4981 gtttcggcgg aaaaaccggg catcagcggc atattggatt tggtggaaat ggatacaaaa
     5041 acaggccgtc tgaaacctgt ggaatacaaa cgaggcaagc ccaaacctga cccggtggat
     5101 gaaatccagc tttgcgccca aggcttgtgc ttggaagaaa tgacggggca aaccgtctct
     5161 gagggcgcgc tgtggtatat gcaaacccgc caccgtgtcc ccgtcgtgtt ttcagacggc
     5221 ctgagacctt tgcaataaca taggttacta aaattttatg ctcaatctca ttttcaaaat
     5281 gcaaaacttt tctgattttt cctacttttt gctcaatatt aggaaggttt taggcaattg
     5341 aaaatttttt ggcgcatttt tatgcgtcaa atttcgttaa cagactattt ttgcaaaggt
     5401 ctcggcctac gcgcccaaac cctcgcaact atagccgccg tccgcgaact cttaaacagc
     5461 ggacaaaccc cgccgcccga ctacggcaaa cgctgcaaag cctgctctct ggtggaagtc
     5521 tgtcagccgg agttgttgga gaaacgggat gggagtgtgg gatatgtgga agcgttgttt
     5581 attgtgtaag aaaaaaccgc aggtatttga aacctgcggt tttttggttt acagcttgct
     5641 gtcttcgctg tccaagaaac ttctcaaacg gtcgcttctt gtcggatgcc gcagcttgcg
     5701 gagtgctttt gcctcgattt gacggatgcg ttcgcgcgtt acgtcaaact gtctgccgac
     5761 ttcttccagc gtgtggtcgg tgttcatatc gatgccgaaa cgcatacgca ggacttttgc
     5821 ctcacgcggt gtcaggcttt cgaggatttc tttggttact tcgtgcaggc tggtgtacat
     5881 tgccgcatcg gccggcgcaa cattgttggc atcttcgatg aagtcgccca agtgcgaatc
     5941 gtcgtcgtcg ccgatggggg tttccatcga aatcggctct ttggcgattt tcatgatttt
     6001 gcggattttg tcttcgggca tctgcatcag ttcggcaagt ttggcggaat cgggttcttc
     6061 gccggtttct tgaaggtgtt ggcgcgagat gcggttcatc ttgttgatgg tttcaatcat
     6121 atgtaccgga atacggatgg tacgcgcctg atcggcaatc gagcgggtaa ttgcctggcg
     6181 gatccaccag gttgcgtagg tggagaattt gtagccccgg cggtattcga acttgtcgac
     6241 cgccttcatc aggccgatgt tgccttcctg aatcaaatca aggaactgca agccacggtt
     6301 ggtgtatttt ttggcaatgg aaatgacgag gcgcaagttt gcctgaatca tttcctgttt
     6361 ggcggctgcg gtttcttttt cgctcgacac catatttttg ttgatttctt tcaactcttc
     6421 gatggaaatg cgggtttctt tttccatatc cgccaactcg gtttgttttt cgaggatggc
     6481 gtggcggaag cggtcgagtg cgttgctcca aaccctgcct ttggcgattt cttcttcaat
     6541 ccattgcaga ttggtgattt cgggcaggaa gttttggatg aagtagtcgc gttccatatg
     6601 gacgcggtcg aggcagatgt cgcggatttc gcgttcgagt ttgcggatgt tttctacttt
     6661 tccgcgcagg ctgctgctga ggctgtcgat ttgccgggtg gcgaaacgga cttccagcag
     6721 tttgttggca atcgcgtcgc ggtaggcgag atagtctttg tgccggctgt ggtgtttttc
     6781 caaacagccg atcatttttt tgtagtcttt ttcgatttgg gcaaagtggc cgatgacttt
     6841 ttgtttcaat tcggcgagat tggctgccga gacttcatcc gcatcttctt ccgattcttc
     6901 gtcgtcttcg ttttcgtccg aattgtcgtt ggaaggtttc tcgggtgctg tggtttccaa
     6961 gtgccccaag cccaattcgt tgagcaatac ttcattcggg tcgataatgg cttctacgac
     7021 ttcgtcgacg cggatttcgt ctttgcagat tttttcgatg agttctaaga tttccgcaat
     7081 ggaacccggg caggcggaga tggcctgaac catatttttc agggcgtttt cgattttttt
     7141 ggcgatgatg atttcgtctt cgcgggtcag caggtcgacc tgtcccattt cgcgcatata
     7201 catacggacg gggtcggtcg tcctgccgaa ctcggaatct gcgctggaaa gggcggcctc
     7261 ggcttcttcg acggcatcat cgtcggtaac ggcggcattg tcgcttaaca atatgtcttc
     7321 cgcatcaggg gcgtgttcgg taacttggat acccaaaccg gaaatcatac tgacgatgtt
     7381 gtcgatttga tcggcatcgg acatatcgtc gggcagtgcg tcgttgattt cggagtaggt
     7441 gatgtagccg cgttctttgc ccataatgat gagttggcgc aggcgcgcac gttgttcctc
     7501 aatcgtcagg gggcgggcat cgtcttggta ttcttcgtga ttttggtttt tggacattgt
     7561 ctgctttctg ttggcaagat gccgacggtt tgcgtttgga cgcacctgaa ggcgtggcaa
     7621 agtagggtac tctgatgccg tctgaaggct ttttgggatg ggtgttctgc aggtgtcgtt
     7681 gttgcagccg gggtgaatgc ttttcagacg gcataacggt ttacagatgg cggaatccgc
     7741 cggtcaattt tgttttgcgg tcagcagcga cagcaaaagt tttttctcgc tttcatttaa
     7801 gccggattgc aggctttttt gttttaatgt ttcaatttgg ctgtatttta actcattgag
     7861 cagttttttg atgccgattt ggaagttttc gcaatcttct tcgccgccgc cttccatttc
     7921 ttccgattga agggccgacc ggaagatgcg gtcgattgtt tcttcgtagg gcgaaccgcg
     7981 catatgttcc aaaacctgtg cggttgccgg cacggaagga tggtttttaa tggtttcggc
     8041 gaggttggca aggcaggcga aatcgccgtc caacgccaga taatcgggca ggtctatata
     8101 tgcagcccaa tccggattta tcaagaggct gcggatttgc cgttgcacca atgtcggcat
     8161 aacgggctgt ttgacggaaa tcggaggcag tttgtagttt ttttgtttga cgtgccgctt
     8221 tggcgcttcc tgtccgagca gttgcgcgag gttgtcgggg tcgatgccga ccagctcgct
     8281 aagccgttgt tttaacaaat aagccaatgc cggtgcggta atctgcacca aaagcggcga
     8341 actggttttc accaattccg ccttgccttc ctgcgtattg agatgaatgc cgtctgaaag
     8401 gtgttcccag aaatattccg acaaaggctt gctttgattc agaagcgcgt cttcaaattg
     8461 cgctttgccg taggcgcgga tgtagctgtc ggggtcgtgt tcttccggca ggaataaaaa
     8521 atgcagcgat ttgtcgtctt tcaactgcgg cagcgcgttt tccagcgcgc gccaagccgc
     8581 ttttcgcccc gcgctgtcgc cgtcgaaaca gaaataaata ctgtccgcct gccgcatcag
     8641 gattttgacg tgttccgccg tcgtcgccgt gcccaaggcc gccacaccgt agcccacgcc
     8701 gaactgtgcc agcgcgacca cgtccatata gccttcgacc accaaaatcc gttctgcctc
     8761 ttttacagca gcacgcccct catacaagcc gtaaaggttt ttccccttat cgaacaaagg
     8821 cgtatcgggc gaattcaaat atttcggttt cgagtcgtcc agcaccctgc cgccgaaacc
     8881 gatgacctgc ccgcgcggat tgcggatggg gaacataatc cgatggcgga agcggtcgta
     8941 atgccgcccc tcattgtcaa tcaccatccc cgtatccacc aacgcggtat tcggatacgg
     9001 ttggaacact tgcgccaaag gctgccagcc gtcgggcgcg tagcccaaac cataatgcgc
     9061 gatgacttcc gcgctcaagc cgcgtttgtc caaataagct ttcgcagccg gattgaattt
     9121 cagctgctgc gcgtaaaaat cagctgccac cgccgtcgtt tcttccagcg tctgctgttt
     9181 tttcttgcgc tcggcacgga cttcgggatt atcgttctgc ccgcgcactt taggcacaat
     9241 catacctacg cggtcggcaa ggaactgaac cgcctccgga aacgacagtc cctgatgttc
     9301 catcacaaaa ccaatcgccg aaccatgcgc cccgcaactg aaacaatgat aaaactgctt
     9361 ggtcggactg accgaaaacg acggtgtttt ttccttgtgg aacgggcaac acgccatata
     9421 gttcgccccg ccttttttca gcggaacctg ctcgtcgatg atgccgacaa tatcggtttt
     9481 ggccagaagc tcgtcaatga agtcggatgg aatcatgatg tggggggacg ggatgtctgt
     9541 tttcggtacg gacggtacga aatcgggatt tttcggaatg gcggtcggat gtggcggttc
     9601 aggcaaaatg ccgtctgaag ccggaaaccg gatgttcaga cggcatttgc gttcaaacgc
     9661 ttaagccaat ttgccgtggc attgtttgta tttcaaaccg ctgccgcagg ggcaggggtc
     9721 gttgcggtgg acgatttgtc cccgcgcttc caaggcttcg gggctgaaat ccgtcccatc
     9781 gggattaaag gcttcggtaa ccagatcggt ttgcgactga cccaaaagtt cttctatatc
     9841 gggcgattcg gaatggatgg actggatgtt gccgacgggt tgctcttcaa ccgccgcgac
     9901 agggttttgt tcgatttgaa ccgaagtaag cagggaggcg atatggaatt tgatgccgtt
     9961 ccacaggtct tggaacatgg taaaggcttc gcgtttatat tcctgcttcg gatttttctg
    10021 ggcatagctg cgcaggtgta tgccttgtcg caggtagtcc atggcggcaa gatgttcgcg
    10081 ccattggttg tcgatggcct gcaacatcac gttgcgctcg aaatcagcca ttgcctgctt
    10141 gccgaccagt tcggttttgg cggcatattc gttttcgata cgttcgatca ggcgctcttt
    10201 gatgtcttga ccgtcaatcg cattgtccgc cttcagccag gattggatgt cttcctgaag
    10261 cctgaattcg gcagccaggc ggttttccag tgtcgggatg tcccattgtt cttccatgct
    10321 gtcgggcggc atataggtat ccacgaggtc gctgacggca tcagaacgga tttcctgcat
    10381 caggtcgccg atgtctttgc tggtcagaat ttcgttgcgc tggctgtaaa tgactttgcg
    10441 ctgttcgttg gcaacgtcgt cgtattccaa aacctgtttg cgcatatcga agtttctgcc
    10501 ttcgactttg cgttgcgccc cttcgatttg gcgcgtcagc aggttgtgct cgatggcaac
    10561 gccgcgttcg ggggcgaggc ggttgaggat ggcggcggcg cggtcgagtg cgaagaggcg
    10621 caacagcggg tcttcaaacg agaggtagaa acggctggat ccggggtcgc cctgacggcc
    10681 ggaacgtccg cgcaactggt tgtcgatacg gcggctttcg tggcgttccg taccgatgat
    10741 gtgcaaaccg cctgcttcca tcactttatc gtgttctgcc tgccagccgt tttcgagtgc
    10801 ggcgatttgt gcctgtttct cttcgtcgct caaggtttcg tcggcgcgga tggcatcggt
    10861 ttggtgcttc aggttgccgc ctaaaacgat gtccgtaccg cgtcccgcca tattggtggc
    10921 gacggtaatt gcgccgactt tgccggcttg ggcgacaatc agggcttcgc gttcgtgttc
    10981 tttggcgttg aggacgttgt gcggcagtcc ggctttttgc agaaggcggg agaccagttc
    11041 ggagttttcg atgctggtcg taccgacgag gacgggctgc ccgcgtttgt ggcattcctc
    11101 gatgtctttg acgacggctt cgaatttttc ttcggcggaa cggaaaatct ggtcgttgaa
    11161 gtctttgcgc tgtacggggc ggttggtcgg gatgatgacg gtttcgaggt tgtagatgct
    11221 ttggaactcg aaagcctcgg tatctgccgt accggtcata ccggagagct tggtgtacag
    11281 gcggaaatag ttttggaagg tgatggaggc gagggtttgg ttttcgcgtt tgatttccac
    11341 gccttctttg gcttctacgg cttgatgcag gccttcggac cagcggcggc cggacatcag
    11401 acggcctgta aattcgtcaa cgatgacgat ttcgccgtct tggataacgt aatgttggtc
    11461 tttgtggaac agcgagtgcg cgcgtaatgc tgccataagg tggtgcatca gggagatatt
    11521 tgccgccgag tacagcgagt cgttttctgc cagcagcccc atttgggtca ggatttgttc
    11581 ggcgtgttcg tgacctgttt cgctcaggat gacctgatgt gccttttcgt cgacccaata
    11641 gtcgccttcg ccttcttctg tctcttgacg gacgaggtgg ggcggaacgg tgttcatgat
    11701 ttggtagagc tggatgttgt cgtctgcctg accggagata atcagcgggg tgcgcgcttc
    11761 gtcaatcaag atggagtcca cttcatcgac aacggcaaaa ttcaattcgc gctgcacttt
    11821 gtcgtattgg tcggttacca tattgtcgcg caggtagtcg aagccgaatt cgttgttcgt
    11881 gccgtaggta atgtcggcag cgtaggcgtt ttgacggtcg aacggctgca tatctgaaat
    11941 aatcacgccg acggtcagac cgaggaaatt gtagagcggc tccataatgc ctgcgtcgcg
    12001 cgaggcgagg tagtcgttga cggtaacgac gtgcacgcct ttgccggcca gcgcgttgag
    12061 atagacggcg agggtggcga ccagggtttt gccttcgccg gtacgcattt cggcgatttt
    12121 gccgtcgtgc agcaccatac cgccgataag ctgcacgtcg aagtggcgca taccgagtac
    12181 gcggcgggac gcttcgcggc agacggcgaa ggcttcgggc aaaatgccgt ccaaagtctg
    12241 accgtcggcg aggcgttgtt tgaattcggc agttttggct tgcagatcag catcgcttag
    12301 ggcttgcatc tgttcttcga gcgcgttgat tctggcaacg gatttacggt attgtttcag
    12361 caagcggtcg ttgcggctgc cgaagatttt cttggcaatg tttgtcagca tgaataattc
    12421 ctgcgcctga tataatagtg gggcgaaaac gcgattttaa cacaagcgtt tgtgcaaagt
    12481 gctaatgccg tctgaaataa aggcgcgttt gcggtatttc aatcttttgg ggtgtatgtt
    12541 ttatgaattt ggaacagttg ggcaggcggg acgcgctgct ttccggactt ttgaaacagg
    12601 cgggacagtg gcggcggttg gatgctgccg tgaaaaaact gctgcctgcc aacctgcacc
    12661 cccactttca gacggcatgt atcgaagacg gcaggcttgt ccttttggcg gcaaacaata
    12721 tggcggcatc gcgtttgaag atgattgccc cgtcggtatt gccgcagttg gcagggcttg
    12781 atgcttcgat acggtctgtt tcggtcaggc tggttccaaa accggaaaaa ccgccgaaaa
    12841 ccaataccct gcatttgagt aaggctgcgt tggagagttt cgattcggcg gcggcaaagt
    12901 tggaagaacg gcatcccgaa ttggcggagg cactggagga gctggtcagg aagtacgggg
    12961 cataaggcgg ccggcaggga tgttgtaaat atccgttaat atttgcgcgg cgggggacgg
    13021 ctgccctaat attaaagaaa taggttcggg gtaagattgc cctttttttg ggtaaacgat
    13081 tgtaaacttg caaacaggct ttgatttcaa atgaagtttg tagcaaaatg ccgccccgaa
    13141 acatctgttt gtgcaacacg gcggaatctt tttcaaggtt ttgttaatgg cggttgcact
    13201 ttgatttccg taaaaccgaa tattatttta tcgattggag atttaccatg aaagcttatc
    13261 tggctctgat ttctgccgcc gttatcggtt tggctgcctg ctctcaagaa cctgccgcgc
    13321 ctgctgccga ggcaactcct gccggtgaag cacccgcttc cgaagcgcct gccgccgaag
    13381 ctgctcctgc agatgctgcc gaagcccctg ctgccggcaa ctgtgcggca actgtcgaat
    13441 ccaacgacaa tatgcagttc aacaccaaag acatccaagt cagcaaagca tgtaaagagt
    13501 tcaccatcac tctgaaacat accggtacgc aacccaaagc cagcatgggt cacaaccttg
    13561 tgattgccaa agctgaagac atggacggcg tatttaaaga cggcgtaggt gctgccgata
    13621 ccgactatgt caaacctgac gatgcgcgcg ttgttgccca caccaaactg atcggcggcg
    13681 gcgaagagtc ttccctgact ttggatcctg ccaaattggc tgacggcgac tacaaatttg
    13741 cctgcacctt cccgggtcac ggtgctttga tgaacggcaa agtgactttg gtcgattaat
    13801 ccgcttaaag tttcaaaaga cagacagcct gctttgtgca ggctgtttta ttataaaatg
    13861 actgcttgaa aagtccccgt tgagaacgaa aacatgaatc cgtttgaaac ccaaagcgtt
    13921 acctttgccg aaccgattga aatgctgtat gcctgccacg gcaaagtgcg ccgtttttgc
    13981 ggccagattg ctatgttgtc gggctatatc gccgaaaacg gctgcaatca gcttgttttg
    14041 caaaccatcc gccaaatctc ccggtatttc aacgttgccg cgccgctgca ccatgaggac
    14101 gaagaagaaa actttttccc gctgctgctg caatacgcgc cgcaagcccg tgaaggcgtg
    14161 gacgagctgt tgcgccaaca tatcgggctg tacgacaact gggcggctgt ttccgccgaa
    14221 tttgccaaac tcgaagcaga caacgcttat atccccgatg cggaagcgtt caaacgcttt
    14281 gtggagggat atgatgtgca tctggcgatt gaagagcctt tgttcgacat gggcaaaacg
    14341 tttatccccg aagagaagct gaccgaaatc ggcgaaatca tggcggcgcg ccggtgcaaa
    14401 taatgccgtc tgaaacaatc gtctcaagga tatattatgc tgacgcccaa aagctgcgat
    14461 ttgttcaata tcccgttttt ccagtttgcc cagctcaaaa aataccagcc cgagagcatt
    14521 ccgcaaatca aagccgacta caaggaaaac tggcagatat ggcagcagtt gatacagcag
    14581 gtcgccgcag atttgagcga acctttcgcc ccgccgcata tcgaacgctg gtgtaacggc
    14641 tggcaggttc gcgcccattt cttcgcctac ttcaaatacg cccaatacaa aaattccgcc
    14701 gccatcctgt cgatactgct caaccgccgc cgcctgagcg tcagcttgga ctggcattgc
    14761 tacaaagccg acgtgtcgcc gattgcgctg cccgagtaca accgctggct ggatgatttc
    14821 gataccgaaa aatacgccgc attcgatatg tggcacggcg cggaaagcga atatgacgac
    14881 taccgcaccg tcgcacaaca aagcgaatcc gaccgaaggt tgcaaaacga cgaagacttt
    14941 ttctgcatcg gcaaacacat tgagcgcgac gatttgggca agcaggatgt agtaaaatgg
    15001 atagccgaaa cggtggaaga tttactgccg ctttacgaag cctgtcacgg caaatgattc
    15061 agaaaactga cgggtacgaa tgcccgtctt atacaccaat ccaacccgga ggtcgtctga
    15121 aaatcagctt caggacattt cagacgacct gatttccaaa gtgaacaaaa tgaccgaaac
    15181 ccaatcccta gaactcgcca aagcgttgat ttcccgcccg tccgttaccc ccgacgaccg
    15241 agattgccaa aaactgcttg ccgaacgcct gcacaaaatc ggttttgcgg ctgaagaact
    15301 ccatttcggc gacaccaaaa acatctggtt gcgacgcggc acgaaagctc ctgtcgtctg
    15361 ttttgcaggg cataccgacg ttgtgccgac aggtcctgtt gaaaaatggg attcgccccc
    15421 gttcgaaccg accgagcgcg acggaagatt atacgggcgc ggcgcggcgg acatgaaaac
    15481 cagcatcgcc tgtttcgtta ccgcctgcga acgctttgtt gccgaacatc ccgaccatca
    15541 aggcagcatt gcactcttga ttacttccga cgaagagggc gatgcactgg acggcacgac
    15601 caaagtcgtc gatgtgttga aagcacgcgg agagttgatt gactactgca tcgtcggcga
    15661 accgaccgcc gtggacaaat tgggcgatat gattaaaaac ggccggcgcg gttcgctgtc
    15721 gggcaatctg accgtcaaag gcaagcaagg gcatatcgcc tatccgcatt tggcggtgaa
    15781 tcccgtgcat acttttgccc cggccttgtt agagttgacg caggaagttt gggacgaagg
    15841 caacgaatac ttcccgccga ccagctttca aatttccaat atcaacggcg gcacaggcgc
    15901 gaccaacgtc attccgggcg agctgaacgt caaattcaat ttccgcttct ccacagagtc
    15961 caccgaaaca gggctgaaac aacgcgtcca cgccattttg gacaaacacg gcgtgcaata
    16021 cgatttgcag tggtcgtgtt cgggacagcc cttcctgacc cacgcgggca aactgaccga
    16081 cgtggcacgc accgccattg ccgaaacctg cggcgttgag gccgaattgt ccaccaccgg
    16141 cggcacttcg gacggacgct tcattaaagc cattgcgaaa gaactcatcg aattaggccc
    16201 gtccaatgcc accatccacc aaatcaacga aaatgtgcgg ctggacgata ttccgaagct
    16261 gtcggcggtg tatgagagga tattggcgcg gttgttggct gaaaaggccg tctgaaaaga
    16321 agatgggctg gttgtcggat atggatgtcc gacaatctga ttagacccaa catgagtttg
    16381 agataacgca ttgattttta tgtagagcga tttatttgaa cggtaatcca ttccctctcc
    16441 cgtgggagag ggttagggag agggcattgg gacggcaacg gaaatattgt ctgtatctgc
    16501 cacgttttac cctccctccg accctcccca caggggaggg agctaagttg caggcacacc
    16561 gacggaatat ggttgaaacc ctgcccttca gggcagtaga attttggttt attttttggg
    16621 aagtaacccc ttccaaatca gggtaaacag ggctgcgcct tatgtgcggc cctgtgtgtt
    16681 gaaacattga ggaatataac cgtgccgtca aattactttc atacgcgtga acgattgggt
    16741 gctttaggcg gcatcgtgca acatcatacc gccttgtacc gcagcgtggc ggtatatgaa
    16801 ccgaccgtgg ttatcgtgcg gcgcggatgc aagaagctgc ggtgggcggg gcgcgaattg
    16861 aggattgcgg cgggtgaggc tgtcggcttg gcaggcgggc agacgcttga cgtcatcaat
    16921 attcccgatt cagacggcct ctatcaggcg caatggattg ctttcgaaca ggaaaccgta
    16981 gaaaggttcg ccgcacaata cgggacggcg caggcagtgt gcgatgcggt gaagctgccg
    17041 caccccgggc gtatgggcgc ggcgtttgat tatgcggctg cggtactggc ggatgaagaa
    17101 gtgccgcaca atgcggcgga ggccgcgctt tgcggcgtgt tggcgtggtt gcagcatgac
    17161 ggcatcggtt ttgccgtgta cggaggcgtc aacctgatgc ggcaaatccg caaactgatt
    17221 actgccgata tggctgcgga ctggtcgtcg gcaatgctgg cgcagcggct taattgcagc
    17281 gaggccgcgt tgcggcggcg gttggcgcgg caggacacga atttccgtac gctgctgacg
    17341 gatgtacgca tgatgcgcgc gctgacgctg ttgcaggtta cgcaatggtc ggtagcgcag
    17401 attgccggcg cggttggcta tgactgcccg tcccgtttca gcgcgcgctt caaagagctg
    17461 ttcggttgcg taccgtcggt ggtccgctcg gaagcggagc cggcggcata tcggcgcacg
    17521 gggcaggtca gcgtcggtgt tcgaccgtga atttcgcttc gcccaagctg tttgccttag
    17581 tggagaaacc gaccagcacg ggtgtggatt cggcggtgat gttcggcagc ttggcgactt
    17641 tcagcgcggt aagcgtgaat tcgtagcggt gtttcctgcc ttcgggagga cacgcgccgc
    17701 cgtagcccgg cgcgccgaag tccgtgcggg tttgcagcgc gcctttgggc ggcctgcctc
    17761 attgtgcggt aatgcctgcg ggcaggcggc ggacatcggc gggaatgtcg gcgaccaccc
    17821 agtgcatccg gtccagaccg atcggtgcgt ctttatcgta aacggtcagg acgaaacttt
    17881 ttgtccctgc gggcgggttt taccacgaca gcgcgggcga agcattgccg cccgaacagc
    17941 cgaagccgta aggcgcgctc aaaagctggt tttgcgtgaa gccgccgtct tcggacgggt
    18001 tgtcgaattg cagcgtgaat gcgcccccgg caaaggcagc ggttgaaaaa gccagcagta
    18061 cggcggagag gatgcgggtt tgcatagatg attcctttgc ttgatgaata agtggattca
    18121 ttataggctg tcaacggcgg caggaatgtg ccgaaacggt tggatgatgt gccggaatgc
    18181 gcaaatgcaa aaatcgtggt ttcagacgac cctttgaagg ccgtctgaaa tttttcatag
    18241 ggcgacgctt tatgtgtcgt cctgtgtgtt gaaacattcc tttccctgat gctttggaaa
    18301 tattgtatat ttaggcattg ccgcaaccag accctcaagg tttccgccgc agcctgttcc
    18361 tgtaccggcg tgcgggaatg gcggaaatcg gggatttcgc cgtcaaattt atgataggga
    18421 tacggagcaa acgaatgatt accctgcctt ccttaaacaa tctgccgtca aaatgggatg
    18481 aaatccgcca ttgcctcgaa acccgggttt ttgagtgcgg cgttatgccg tatcccaatc
    18541 tgacggaatg cgaagccggg cagttcgagc gggatttttg ggacaatatc ggctgcgcgc
    18601 ctgaagaata tgtccgtatc cgccgcgcca tccgtctgtt ggaattacgt tatcccgaca
    18661 gcttgaacga gctggtgtgt gccgccattg ccacgccttt gggcgagatg ttggcggtgt
    18721 tcggcgttaa gggattgtgc ctgctggagt ttgtcggaca gaagtatttg gaacaggaaa
    18781 ttgccgccgt ccaaaaagcc ttgcgcggac ggtttgtgtt tcgggaagac gggcggatgc
    18841 aatttttgcg tcaggaattg gatttgtatt tccaatgccg tctgaaaacc tttgccacgc
    18901 ccttggaaat gattggtacg gcgtttcaaa aacaggcgtg ggacgtgctt ttggcgattc
    18961 cttacggcga aacgcgcagt tataaggagc aggcgcagcg tttgggcaac cccaaagccg
    19021 tccgcgccgt tgccgccgcc aacgggcaga acaaggtgtc tgtcatgatt ccctgccacc
    19081 gcgtcatcgg cagcgacggc aaactgaccg gctacgcggg cgggttgaac cgcaaacagt
    19141 ttttactggc tttggaacgc ggcgaagttc agacggcatt gttttaagcg gggaaatgca
    19201 gaacggctca tcgggcttaa atatggtgtg atgacggctg tgaaaattca tacaacggca
    19261 atccttttaa ggcatcggtc ttaatgtctt tctgattcat atgcttgtcg ttttgtcgcc
    19321 catttttcca aaattaagga ttgacacatt ttttccggac gctataatac gccccattcc
    19381 ccgatagctc agtcggtaga gcgacggact gttaatccgc aggtccctgg ttcgagccca
    19441 ggtcggggag ccagaatttt gaagcccaaa cttgaatgtt cgggcttttt cacatttgca
    19501 cgtccggcgc tcggaatgcg gcgagatagg ttttccaacg gtttcgcggt aaaattctgc
    19561 ctgtctgatt tcggtttcgt tttatgtcta tcaaaatcct gattatttct cccagttgga
    19621 taggcgactg cgtgatgacc cagcccttgt tccgccgttt gaagaaactt catcccggtt
    19681 gcacgattga tgtgttcgca ccgaagtggt cgatggcggt gttcgagcgt atgccggaag
    19741 tgaatgagat tcttgaaaat ccgttcggac acggtgcgtt ggagctgaaa cgccgttggc
    19801 gggtcggcag ggagttggga cggcgcggat acgacagggt tatcgtgttg ccgggttctt
    19861 tgaagtcggc agtcatcgcg ctggcaacgg gcatcggaaa aagaacgggt tatgtcggcg
    19921 aaagccgtta ttttctgttg aacgatatac gcaggctgga taaggaacgt ctgcctttga
    19981 tggtggaccg atatacggct ctcgcgcatc agagtcagga agattttgac gggcattcga
    20041 gattccccga gttttccatt gatgaacggc ggcgggaaat ttctgtcgaa actttcggtt
    20101 tgaatcttgg aaagcctgtt ctggcttttt gcccgggtgc ggaattcggg ccggcaaagc
    20161 gttggccggc aaggcatttt gccgagttgg gcaaacacta ttcggaggcg ggttggcagg
    20221 tttggttgtt cggttcgcaa aaagataatg aaatcgccga agaaatcaac tgcctttcag
    20281 acggcatgtg tgtcaatttg tgcggcaaaa ccgatttgtc gcaggcaatg gatttgctgt
    20341 cgttggcgga cacggtcgtg tgcaacgaca gcgggctgat gcatttggcg gctgctttgg
    20401 gcaggaaggt agtggcggtt tacggttctt ccagcccgac gcatacgccg cctttgagcg
    20461 acagggcgaa aatcgtcagc ctgcacttgg aatgttcgcc ctgtttcaaa cgtgaatgcc
    20521 cgttggggca taccgactgc ctcaacaggc tgtatcccga gaagattgtg caggcggttg
    20581 aagaggcggt atgatggctt ctgttttcgg atttataccg tctgacggcg gatattgata
    20641 ggatgtttat ggctattgcg aacaataaaa aagcatttca cgattttttc attgaagacc
    20701 ggattgaagc cggtttggtc ttggaaggct gggaagtcaa agcaatccgc gccgcgcgcg
    20761 tacagcttaa agagagttat atctattgga aaaaagatgc gttttatctg gtcggctgcc
    20821 atattacggc tttgcctacg gcttcgacac acatcacgac acacatcaaa ccggatgccg
    20881 tacgtccgcg caaactgtta ttaaaacagt cggaaatcaa taagttaatc ggaaaaaccg
    20941 aacgtgcagg ttacaccatc gtgccgctgg atttgcattt ttcacgcgga aaaatcaaga
    21001 tggaaatcgg tttggcaaaa ggtaagaaac agcacgataa acgacaaagt atgaaggaag
    21061 ccgactggaa acgcgagaaa cagcggttga ttaagcatac gcgctgattg tggaaacaat
    21121 gcttcagacg gtacgggcaa ggctgccgcc tgatgcgtaa acgttctaca cctaaaccag
    21181 catccgcttt aatcagcacg gcatgatgcc ccaaaccttc gtcaaaggtt tgagcaattt
    21241 gatgataaat tggcattagc caagggtaaa gtgcggtcat ttttcctgaa atttttgtaa
    21301 ataacgaggg caattataca cattttcaaa ccggcttgac aaaatagcaa aaagcaaacg
    21361 tttactttta ataaataaga aataaataga gaaaatttga actaaatgac attttttgtt
    21421 cagttttatg ctaaaatcca cgccctattt atttttactt gacaaaggaa aatttattat
    21481 gaaactcgaa gcaagcaagc agaagtttaa aaaatcattt attataagtc tatttttttc
    21541 tattctttaa tacctctccg cttttggctg ttgattatgt ttacgacaaa accaagctca
    21601 ctgatgatga aattatccgc ttaaaaaaaa ctccgcgata gaaatagtga atattggaaa
    21661 gaagaaactt atcacataaa aagtaacggg cgaacttatc caaacattcc cgcattattc
    21721 cctaaacatc ctttcgatcc attcgaaaac atcaacaact caaaaaagat ttctttttat
    21781 gacaaggaat acactgaaga ttaccttgtt ggcttcgccc gaggtttcgg ggttgaaaaa
    21841 agaaatgggg aagaagaaaa accactccgg caatatttta aggactgtgt aaatactgag
    21901 aattccaata atgataattg taaaatttca tccttcggaa actatggccc aatattaata
    21961 aagagcgata ttttcgcctt agcatcacaa ataaaaaata gccatatcaa cagcgaaatt
    22021 ttatctgtag gtaattatat cgaatggctt cgcccaacac ttaataagct tactggttgg
    22081 caagagcatc tatatgctgg tttagatcca ttccactata ttgaggttac agacaactcg
    22141 cacgttatcg gacaaacgat tgatttggga gcattagaat taacaaattc tctatgggaa
    22201 ccccgttgga actctaatat tgattattta ataacaaaaa atgctgaaat tcgtttcaac
    22261 actaaaaatg aaagtttact cgtaaaagaa gattatgctg gcggagctcg ttttcgtttt
    22321 gcttacgatc taaaagataa agtccctgaa ataccagttt taacttttga aaaaaatata
    22381 actggcacat cagatattat ttttgaggga aaagcgttgg ataatttgaa acacctagac
    22441 gggcatcaaa ttgtcaaagt aaatgacacc gcagataaag atgcctttag actttccagt
    22501 aaataccgaa agggaattta tacgctctct ttacaacaac gcccagaggg cttttttacc
    22561 aaagtgcaag aacgcgatga tattgcgatt tatgcacaac aggctcaagc cgccaatacc
    22621 ttattcgcct tgcgtttgaa cgacaaaaac agtgatattt tcgaccgcac tttaccacgc
    22681 aaaggcttgt ggttgcgtgt gattgacgga cattccaacc aatgggtgca aggcaaaaca
    22741 gcacctgtag aaggctatcg aaaaggtgtg caactcggcg gcgaggtgtt cacatggcaa
    22801 aacgaaagta atcaactttc tattggctta atgggcggac aagcagaaca acgcagcact
    22861 ttccgcaatc cggatacaga caatcttaca acgggaaatg tgaaaggctt tggtgcgggc
    22921 gtttacgcca cttggcatca gcttcaggac aaacagacag gtgcgtatgt tgatagctgg
    22981 atgcaatatc aacgtttccg ccaccgcatt aacactgaat atgctacaga acgttttact
    23041 tcaaaaggta ttactgcctc aattgaagcg ggttacaatg cgttattggc ggaacacttc
    23101 actaaaaagg gcaacagcct tcgtgtttac ctacaaccac aggcgcaatt gacttatttg
    23161 ggggtaaacg gaaaattcag cgatagcgaa aatgcccaag tgaatttgct tggctcccgc
    23221 caattacaaa gccgagtggg cgttcaagct aaagcgcaat ttgctttcac taatggcgtc
    23281 actttccaac catttgttgc tgtcaattca atctaccaac aaaaaccttt cggtgtggaa
    23341 atagacgggg atcgtcgagt gataaacaat aagacggtaa ttgaaactca attaggtgta
    23401 gcagcgaaaa tcaaatctca cttaaccttg caagcctcat tcaaccgcca aacaagcaaa
    23461 catcatcacg ctaaacaagg tgcgttgaat ttacagtgga cgttttaagt taaattgtaa
    23521 caaaataaaa tcggcaggga aattcactgc cgattttatt attttaaaag tgcggtcatt
    23581 tttcgtgtaa tttccaccaa tttttaccgc actttcaatt tcagcttgaa caagttcaat
    23641 agtctgctct gcattaatta cgactgcttt tggattatct tttactaatt ctaaatagcg
    23701 tgctcgagtg cgatggaaaa aatctaaatc catttgctca atacggtcta actcgccacg
    23761 tccacgagct cgcgctaaac cgacaatcgg atctattgtc cttttctttg ttgtgtatgc
    23821 aaatcagaaa tgccggttac ggaagcagtt tgccaggatt cataatgtta tatggatcaa
    23881 ggtgttgttt gatgcttttc atcagggcaa tttcagaggg cgtgcggaca gcgggcagcc
    23941 attgtttttt gatgatacct atgccgtgtt cggcggcaat cgtgccgttg cgggcaagga
    24001 cgttgcgata gactgtgctg ttgatgtcgt tttcgtaacg gtaaacttca ttgctgagga
    24061 tttcgggcgg aaaagtattg tagtgcaggc tgccgtcgcc cagatgtccg aagcagacga
    24121 tttgtatgcc tttaaaattc tgttccaaat ctttggcgca ccggcggaca aagtcggcaa
    24181 cgcgcccgat aggaacggca atatcgtgtt tgatgctggt gcccagtttg cgttgggagg
    24241 cggagatgtt ttcgcgcaac gcccacatat ggatacgttc ttgttcgctt tgcgccaata
    24301 cgctgtcggt aaatcctttt ttataaagaa attcgacaag ccgatcatca agattgctgt
    24361 cgggtaatga gtcggtcaac tcaagtaaaa tatgccattc tgaatgtgtc ggcaggggga
    24421 gtttgctgaa ttcggaagac aactcggcgg caaaacggcc gatcagttca aaactgcata
    24481 agcgttcggc aaagtgcgct tgggtttcgg tcagcaggcg gacggcggat tcgatgtcgg
    24541 gaatgccgac ccatgccgtt gccttgtccg agggacgggc aaaaagcttg agcgtggcgg
    24601 cagtgatgat gcccagtgtg ccttcgctgc cgataaacag atggcgcagg tcgtagccgg
    24661 tggtgttttt atgcagggga tggagatggg aaaccagttc gccgttgggg aggacgactt
    24721 ccaaaccgat aaccaggtcg cgcatcgtgc cgtaacgcaa tacgttcaaa cctccggcat
    24781 tgcaggcgat gttgccgccg atttggcacg agccttcgct ggcgagactg agtgggaaca
    24841 gcctgtttga ggcttcggct gcctgttgga cggtttggag tacggaacct gcttcgacgg
    24901 ttatgcagtt gtctgacaaa ttgatgctgc ggatgcggtt gagtttggaa aggttcagca
    24961 atacgccgtt ttccgatact gccgcgccgc acaaaccggt attgccgcct tgcggcgtaa
    25021 ccggaatgcg gtgttcgtaa caaaaacgca taatggtttg cacgctttca acgctgcgcg
    25081 gctgcaaaat gatgtctggt gcagacgtaa agcggttgcg ctgatctttt aaaagcgtcg
    25141 gagaaggttc ggcaatttca tctgccggca ggagtcggga aaattcggta tgaaggtcag
    25201 gcatgtttgt cggaagggta gggagaagat tgaggaaggg aatctgatgc cgtctgaaaa
    25261 atcaaaaaag caggatactg aacgtatcct gcttttttgc aagcggaaaa tgcttatttt
    25321 gcagcttcgg cagcaggagc ttcagcggca ggtgcttcgg cagcaggagc ttcagcggca
    25381 ggtgcttcgg tagcagcagc ttcggcggca ggtgcttcgg tagcagcagc ttcggcggca
    25441 ggagcttcgg tagcagcagc ttcggcggca ggtgcttcgg cggcgggagc ttcggcggca
    25501 ggtgcttcgg cggcaggtgc ttcggcggca ggtgcttcgg cggcaggtgc ttcggcagca
    25561 ggtgcctcgg tagaggaagc ctcagcagca ggagcttcag cggctttttc tccgccgcag
    25621 gcagccaaag ccaaagacaa caaagcagcg gcaaacaggg attttttcat tttagtaacc
    25681 tttaaaatca atttaaatta ttgaaacaaa taggtctttt tcgaccatac tacaaaccgg
    25741 gttggaaatt catcgctgaa tttgcgtaat tatgcaccat ggaataaggg attgcaatgt
    25801 cgtacagatg ggaaaaaggt ttgaatgaaa agttttctgg cgggcggatt gataaatcct
    25861 gtaaaacggt tttgctgtta aaaatccgat agggataaat attatagaga ttaatataat
    25921 tcagtatctt gtatgattta ttaaggcaga ttggggaagt cggcagatgg gtcggaatgg
    25981 tttttgagag tgttgtaaaa aatttacact gcaataaaat aacaaaaatt tacattgaaa
    26041 tatgtgccgt acagtttatg aacaggttaa cggtaatcgg ccggcgcggc ggttgtgtta
    26101 gaatgcgcct gtaatcaatc tgctgttcct gaaaggacaa atatggctat tgcaaaaaat
    26161 tccgtggttt cgctgcatta tgagatgtat gatgccaaca atcagctttt ggataaaacc
    26221 gaagaaccga ttgcgtatct gcacggcggt tacgacggca ttttcccttt ggtggaagag
    26281 gcgttgcacg gtaaggatgc cggcgatacg gtcgatgtgg cgctttcgtc cgacgatgcg
    26341 ttcggcgagc aggattcgga gttggtccgt attgaagatg cgggcgcgtt ccctgttgaa
    26401 gtcgaagtcg gcatgatgtt tgaagccgac gatcctgaaa ccggcgatgt tgtcgtctat
    26461 cgtgtaaccg atgttgccga cggcaaggcg gtggtggacg gcaaccatcc tttggcaggc
    26521 atgaaaatcc gctttaaagc tacggttgaa agcgtgcgcg atgcatccga tgaggaaatc
    26581 gcacacggtc atgtccacgg ttcgcacggt cattaccacc actgattgcc ggcagcttgg
    26641 aggtatctga tgccgtctga aaagggtttg ttccgtttca gacggcattt tgtttgatgg
    26701 atggagaagg tatgtcggtt gggcattatg agaattttcc cgtcggttcg ctggttttgc
    26761 cgcgcaggtt gaggaagccg gttcatgcgg tgtatgcgtt tgcacggacg gcggacgata
    26821 tggcggacga gggcagtatg ccgtctgaag ccaggttgtc ggggttggag ggtttgcggc
    26881 gcgagttgga cgtgttggca tcgggcggcc ggtcggcgca tcctttgatt gcacgattgg
    26941 atgccgaggc ggttgtgccg ttcggtttgg atttgcagcc gttttatgat ctgctctcgg
    27001 cgttttcgca ggatgtggtt aaaacgcggt acgcgcattt cggcgatctg accgattatt
    27061 gccggcgttc cgccaaccct gtcggacgca ttatgctggc tttatacggg aaaacggatg
    27121 cggtgtgcgt agcgcaaagc gacggtattt gtacggcttt gcaactggtg aatttttggc
    27181 aggatgtagc tgtggattgg caaaagggca gggtttatat cccgcaggac gatttgttga
    27241 aattcggtgt ttctgaggaa cagatcgcag caggacgggc ggatgcggcg tttcagcggc
    27301 tgatggcgta tgagtgccgg cgtgcattcc gtatgctgaa ggcgggttcg cctttggcgc
    27361 gcgaattgaa agggcgtatc ggtttggaac tccgtatgat tgtgttgggg gcgcagttga
    27421 ttttgcagaa actggacgcg tgccgatacg atgtgtttgc acaacgcccc gttttggata
    27481 agaaggattg gttgattatg ttgaaacgcg cgttgtggaa atgattgggg gaaaatgaat
    27541 gccgtctgaa aggggttgtt ccgcttcaga cggcattttg caggcggtcg gtatggtcag
    27601 gcgatttcat gcgggtttaa cctggcttga tcctctgcac ccaagccgat gagtttgcgc
    27661 agtcgggtca tgtagaaatt gacatccaaa ccggtgccgt agcgttgcgc cgtccacagt
    27721 gtttccgcca gtgcctccat catttcgtgt tcggcttccg gccagccgcg tttggcgcac
    27781 agggtgtcgt ggatggcgcg tatgccgtgc ggctggtcta tgcccgcctg ttcttggacg
    27841 gacaaatgca gcgacatatg caggaagggg ttgctttcgc cgttttcggg cagccagtcg
    27901 gtgtccagat ggtcttcgat gcgttcgaga taacggtggt attcgggatg ggcttcgaca
    27961 atgcggaggg ctttctgttc caacgcgccc aattgcagcg gattgagccg ctgctgccac
    28021 acgcgggcga aaaagcggcg gacatcgtgg gtattgacat cgtacatggc gggtccgata
    28081 agatgtttat ggaaataggt tcggataatg gcgggtttta cataaattaa atcctaccgt
    28141 caaccttacc atttataatg cggacaaatt atttgtctga aacggattcg attatgaaaa
    28201 aactgatttg cctgttcgcc gtatttttga tgttgtgcgg acgggctttc gcgctggatg
    28261 cgaacgatct gctgccgccg gaaaaggcat tcgtgccgga gcttactgtt gccgacgacg
    28321 gcgtgaacgt ccgtttcagg attgccgacg gatactatat gtatcaggcg aaaatcgtcg
    28381 gcaagaccga tccggcggat ttgttgggac agccttcttt cagtaagggc gaagagaagg
    28441 aagacgagtt tttcggcagg cagacggttc accatcacga ggcgcaggtt gcctttcctt
    28501 atgcaaaggc tgtcggcgaa ccgtataaat tggttttgac ctatcagggc tgtgccgaag
    28561 ccggcgtgtg ctatccgccc gtggataccg agtttgatat ttccggcaac ggcacttacc
    28621 atccgcaaac cgacgaaccg gcatccgcca aagaccgttt tttgcagcct tcctctcaaa
    28681 acggcagcgg ggcgttgccg cccccgaagg gggacgaggg cggcgacggc cgtttcaagc
    28741 tgtcttggga tacgctcaac gccaatcttt tggcgttttt tctcgccggt ttgggcctga
    28801 gttttaccgc ttgtatgtat cccctgctgc cgattgtgtc cagtatcgtg gtcggtgaca
    28861 aaaaggcggg caaggcgcgg gcgtttgtgc tgtccgtcgt ttatgttcag ggtttggctc
    28921 tgacttatac gctggtcggc attgttgccg gactgacggg cgcgctgctg accgtatggt
    28981 tgcagcaggc ttgggtcgtg ttggcggcat cggctttgat ggtcgtcttg gcgctgtcta
    29041 tgttcgggct gttcaacatc cagcttccca acgccgtaca gtcatatttc caaaaccaga
    29101 gcagcaggct ctccggcggt aaaatcgttt ccgtctttat tatgggcatt ctgtccgcac
    29161 tgattgtcgg accgtgcgtc gccccgccgc tggcgtttgc cttgggctat atcggtcaga
    29221 cgggcgatgc ggttttgggc ggtttggcac tttacacttt ggcgttgggc accggcgtgc
    29281 cgctgattgc catcggcacg ttcggaggac atatcctgcc taaggcgggc gattggatga
    29341 atgccgtcaa atacgctttc ggctttatcc tgcttgccgt cgccgtttac ctcgccacgc
    29401 cgcatttgcc ctattatctc gtcgtcgcgc tgtacacgct gctgatgctg gttcctgcct
    29461 ttatgctgct ggtcaacgga cgcaggcaga aacgccgtcc gaaagctgtg gcattcgcat
    29521 tgggcagcat attgctgata ggcggcgcgt ggttcggctg gcagggcgca aacggcaaaa
    29581 cgaccgcgct gcaccatttc ttgaccctca atccgccggc cgaagcaggc aaattttcgg
    29641 aacacggcaa aatgtttgcc gatactgccg cgctgaaggc agcgatggat acggcgttga
    29701 aagaacatcc cgacaaacct gtcgttttgg atttttatgc cgactggtgc atttcctgca
    29761 aagaaatggc ggtttacacg ctcaatcagc cggaagtgca tcaggcagtc gatatggaac
    29821 gctttttcca gatcgacgta accgccaaca cgcccgaaca tcaggcgttg ttgaaagaat
    29881 acggtctgtt cgggccgccg ggcgtgtttg tcgtccgcgc cgacggcagc cgcagcgagc
    29941 cgctgctggg ttttgtcaaa gcagacaagt ttatcgagtg gtatgaacaa aaccgctgat
    30001 tccgatgttt caaatgccgt ctgaagttct cagacggcat ttttgtcaga caggaaaacg
    30061 gcaatatccg ctatacacac ggaagcagtc caaagatgtg aaaaccgtgt gtataagtcg
    30121 gtttgcgtat tgaaattaaa agataaaaat acgatgatta aaaaataggc ataagaaaag
    30181 ataaacacag ccggtgtttg ccggtgtgtg tatcttttgt ggaaaaacat cttaatgcca
    30241 tgtattttat ggaaaataat aacatgatta aaaaatagtc aatcattaag gttcaggtag
    30301 gcggctgcaa ccgtcaatgt ctgaaaagag gtagtgctgt ttaagcatgg aaagcc
//
LOCUS       DAXDXW010000017        30302 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXDXW010000017 DAXDXW010000000
VERSION     DAXDXW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30302
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.017"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(85..2127)
                     /gene="recG"
                     /locus_tag="ACFB64_000675"
     CDS             complement(85..2127)
                     /gene="recG"
                     /locus_tag="ACFB64_000675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC1209082.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLGRLKLPRLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRVSGTAAALRGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTHPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVCLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLSIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPEPNIG
                     LVHGRMKAAEKAEVMAEFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAGESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2213..2656)
                     /gene="secB"
                     /locus_tag="ACFB64_000676"
     CDS             complement(2213..2656)
                     /gene="secB"
                     /locus_tag="ACFB64_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC1209083.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGDPEVDMRVST
                     GSQKLEDGYYDVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRVGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2677..2934)
                     /gene="grxC"
                     /locus_tag="ACFB64_000677"
     CDS             complement(2677..2934)
                     /gene="grxC"
                     /locus_tag="ACFB64_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC1209084.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQRLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3153..4646
                     /gene="rng"
                     /locus_tag="ACFB64_000678"
     CDS             3153..4646
                     /gene="rng"
                     /locus_tag="ACFB64_000678"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC1209085.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSGHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNIEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4681..5932)
                     /locus_tag="ACFB64_000679"
                     /pseudo
     CDS             complement(4681..5932)
                     /locus_tag="ACFB64_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(6014..6643)
                     /locus_tag="ACFB64_000680"
     CDS             complement(6014..6643)
                     /locus_tag="ACFB64_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC1209086.1"
                     /translation="MSASLKDGGYAVDWVKNGAQVAAAAAAQPYDLMLLDLGLPGRDG
                     LDVLSEIRAAGCTVPVLIVTARDDLYSRLNGLDGGADDYIVKPFDMAEFKARMRAVLR
                     RGSGQAQACLSNGALSLNPATYQVEIIAEGRQVALSNQEFSVLQALLARPGVILSRSD
                     SEDKVYGWGGEVESNAVDFLIHGLCKKLGKESIQNVRGVGWLMPRQDAV"
     gene            complement(6911..8224)
                     /locus_tag="ACFB64_000681"
     CDS             complement(6911..8224)
                     /locus_tag="ACFB64_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC1209087.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLIGALLAGFDVS
                     QLKEFYSGGTKSVTQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLSVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPRGGPI
                     GRVASVLGADVGELYKPLLTVQIIGVVFILVLSLFLGVREKRRIVRELGALPAVADLI
                     KPAPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDLVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILISSIAAGIVPLP"
     gene            complement(8602..9174)
                     /locus_tag="ACFB64_000682"
     CDS             complement(8602..9174)
                     /locus_tag="ACFB64_000682"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC1209088.1"
                     /translation="MIVAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGH
                     LPLYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGS
                     KPNADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLF
                     DENGKLIDSARDFVQSYINQFVGLIERNAK"
     gene            9333..10742
                     /gene="dacB"
                     /locus_tag="ACFB64_000683"
     CDS             9333..10742
                     /gene="dacB"
                     /locus_tag="ACFB64_000683"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC1209089.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRAGIPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLRDKGIRNITGRLMLDHSLWGEVGSPDHFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSVKKLMRASF
                     SGNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNRWLLGGGRISDGIGIADTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVAKNI
                     ISGGDGWLDAKLMCKERRA"
     gene            complement(10807..11976)
                     /gene="metK"
                     /locus_tag="ACFB64_000684"
     CDS             complement(10807..11976)
                     /gene="metK"
                     /locus_tag="ACFB64_000684"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC1209090.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPAISHEELSKAVIEQIIKPVLPP
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALV
                     CEHFDLRPKGIVQMLDLLRPIYGKSAAYGHFGREEPEFTWERTDKAASLKAAAGL"
     regulatory      complement(12159..12269)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            12322..13215
                     /locus_tag="ACFB64_000685"
     CDS             12322..13215
                     /locus_tag="ACFB64_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC1209091.1"
                     /translation="MFRLQFRLFPPLRTAMHILLTALLKCLSLLSLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGLNPDTQTVKAVFAETAKCGLELAPAFFKKPEDIETMF
                     KAVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQQLPFHLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTGIQGVKQIIKALRAGEATIILPDHVPSPQEGGGVWADFFGKP
                     AYTMTLAAKLAHVKGVKTLFFCCERLPDGQGFVLHIRPVQGELNGNKAHDAAVFNRNT
                     EYWIRRFPTQYLFMYNRYKTP"
     gene            complement(13260..14324)
                     /gene="tsaD"
                     /locus_tag="ACFB64_000686"
     CDS             complement(13260..14324)
                     /gene="tsaD"
                     /locus_tag="ACFB64_000686"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC1209092.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRSHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYELLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNNICRAFQDAVVEVLEAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPMAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWSLSEIVK"
     gene            complement(14440..15627)
                     /gene="ccsB"
                     /locus_tag="ACFB64_000687"
     CDS             complement(14440..15627)
                     /gene="ccsB"
                     /locus_tag="ACFB64_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC1209093.1"
                     /translation="MTEHYKTLPEHELLIQKSLIRNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAVQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEEKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(15620..17572)
                     /locus_tag="ACFB64_000688"
     CDS             complement(15620..17572)
                     /locus_tag="ACFB64_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC1209094.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWTRIFDF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVRGFQGKTVSREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLAGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGMLVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLRDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPILQDKDYFWLTGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKGQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWRQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVFMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(17850..18473)
                     /locus_tag="ACFB64_000689"
     CDS             complement(17850..18473)
                     /locus_tag="ACFB64_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC1209095.1"
                     /translation="MRRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            18680..19309
                     /gene="yihA"
                     /locus_tag="ACFB64_000690"
     CDS             18680..19309
                     /gene="yihA"
                     /locus_tag="ACFB64_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC1209096.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLRH
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(19430..21826)
                     /locus_tag="ACFB64_000691"
     CDS             complement(19430..21826)
                     /locus_tag="ACFB64_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC1209097.1"
                     /translation="MIKKILTTCFGLFFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRRELYE
                     KYGEDAYTQGFKVYTTVRTDHQKAATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVALDRRALGFAARAVDNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLPQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRPSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRAGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLM
                     LDNSGIAPQPSRRAKEDDEAAVENEQQGRSDETRQDVQETPVLPSNTDSKQQQLDSLF
                     "
     gene            21978..23093
                     /gene="pilM"
                     /locus_tag="ACFB64_000692"
     CDS             21978..23093
                     /gene="pilM"
                     /locus_tag="ACFB64_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC1209098.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIVASVPQ
                     NLATIEQLTYTAKDAELDLQGFVESSISEASSISLEEANYDYQVLSQSAVGEAVLSVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELADEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTAEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAVRQKGIAQTVASQTNADVQ
                     CVHPARYFANDLKTDEQQFELDAPTLTKAFGLAVRGL"
     gene            23096..23695
                     /locus_tag="ACFB64_000693"
     CDS             23096..23695
                     /locus_tag="ACFB64_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC1209099.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINKQSEKNTLLEISIAHLDTELSKIQKLKQEKDAFLIKKNKIGELQIKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            23696..24343
                     /locus_tag="ACFB64_000694"
     CDS             23696..24343
                     /locus_tag="ACFB64_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC1209100.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLVVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKKLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            24361..24906
                     /locus_tag="ACFB64_000695"
     CDS             24361..24906
                     /locus_tag="ACFB64_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC1209101.1"
                     /translation="MKHYALLISFLALSACSQSSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETAKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIILNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            24925..27120
                     /gene="pilQ"
                     /locus_tag="ACFB64_000696"
     CDS             24925..27120
                     /gene="pilQ"
                     /locus_tag="ACFB64_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC1209102.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPFTESVV
                     SVSAPFSPAKQQAAASAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQ
                     HDHIIVTLKNHTLPTALQRSLDVADFKTPVQKVTLKRLNNDTQLIITTTGNWELVNKS
                     AAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNI
                     VASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQ
                     AEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATN
                     TLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGRKKLK
                     NETSAFGWGVNSGFGGGDKWEAQTKINLPVAAAANSISLVRAISSGALNLELSASESL
                     SKTKTLANPRVLTQNRKEAKIESGYEIPFTVTTASGGGNSTNTELKKAVLGLTVTPNI
                     TPDGQIIMTVKINKDSPAQCASGNNTILCISTKSLNTQAMVENGGTLIVGGIYEENNG
                     NTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIIDTAGNSLRY"
     gene            28388..28900
                     /locus_tag="ACFB64_000697"
     CDS             28388..28900
                     /locus_tag="ACFB64_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC1209103.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLIVLPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            28980..30059
                     /gene="aroB"
                     /locus_tag="ACFB64_000698"
     CDS             28980..30059
                     /gene="aroB"
                     /locus_tag="ACFB64_000698"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC1209104.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFIQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMALERAPLTQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAVEAEMGYGVWLHGEAVAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLAHMSHDKKVSGGIMRFIGL
                     NRLGEAVITEITDTDILRRTLQPYL"
BASE COUNT         7660 a         8797 c         7323 g         6522 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta taaaaaatca gatttccgtt acactttttt
       61 ctaatatttc agacggcatt ttgctcacac acccaaataa ccttccctgc cggaaagcca
      121 ccttgccaaa tgcgcttcga cgatttcagg gttttgttca atcagcatcg gggcggtttc
      181 gcgcgcttgt tccaaaaggt gtaagtcttc ttcgagcttg gcgaagcgca gcataggcac
      241 gccgctttgg cgcgcgccga gaaattcgcc ggggccgcgg atattgaggt cttggcgggc
      301 gatttcgaag ccgtcggtgt gttcgtagat gactttcagc cgcgctttgg cgagttcgcc
      361 caagggttcg gcaaacagga ggacgcacac gctttccccc gcgccgcgcc ctacccgtcc
      421 gcgtaattgg tgaagctgcg ccaagcccat gcgctcggcg tgttcgatga ccatcagggc
      481 ggcattgggc acatctacgc cgacttcgat aacggtggtg gcgaccaaga cgttcagacg
      541 gcctgcggca aattccgcca tgacttcggc tttttcggcg gccttcatgc gcccgtgtac
      601 cagtccgata ttgggttcgg gcaatgccgt ctgaagccgg gcgagggttt cggcggcggt
      661 ttgcagttgc agggtttcgc tttcttcaat caatgggcag acccaatatg cctgccgccc
      721 ttttcggcaa gtgccgagga cgaagccttc gacttcggcg cggcggacgt tgttgacgag
      781 gcgcgtttta atcggagtgc gcccgggcgg caattcgtcg atgacggata cgtccaagtc
      841 agcgaagaaa ctcatggcga gcgtgcgcgg gatgggtgtg gcggacatca tcagctgatg
      901 gacttcgcgc cctttgtttt tgagtgcgag gcgttgtgca acgccgaaac ggtgctgttc
      961 gtccacaatg ctcaagccca aattgtgaaa cgccacgccg tctgaaaaca gggcgtgcgt
     1021 gccgacggcg attttgacgc tgccgtcggc gagtttggct ttggcttcgt ctttggcttt
     1081 tttgcgcaaa ctgccaaaaa ggcagacaac ttcaatgccc aaaggttcga gccattgttt
     1141 gaacttgatg aagtgctgtt cggcaaggat ttcagtgggc gccattacag ccacttgcgc
     1201 gcctgattcg atggcggtca gcgcggataa ggcggcgacg atggttttgc cgctgccgac
     1261 atcgccttgc agcaggcggt gcatggggtg ggtttgcgcc atatcgcggc agatttcgga
     1321 aacaactttt tcttgtgcat cggtcagggc aaacggcagg gcttggcgca gggcttgggt
     1381 caatgtgccg tcgccgcgca atgccgccgc cgtgccgctg acgcgcttct gccgcgccaa
     1441 acgcatggac aactgttgcg ccaaaagttc gtcgaatttg agccgctgcc acgccggcag
     1501 cgtgccgtct gaaagttgat gaatagtgaa actcggcggc ggcgaatgca aaaggcgcag
     1561 gctttcggca aggcgcggca gcttcagacg gcctaataaa gcatcaggca aggtgtcgtg
     1621 cagcggcgta acgtccaacg ccgtctgaat gatgcggcgc aaagtgggct ggttcaaacc
     1681 gtttacggtc gggtaaaccg gcgtgaggct ttccgccaaa ccgccgccct cggcatcgcg
     1741 gattttggga tgaatcatct cgtcgccgta aaatccgtgt ttgatttcgc ccacggcgcg
     1801 gatgcgtttg ccgaccgccg tctgcttctg atggctggcg taaaagtgga tgaagcgcag
     1861 aaaaaggacg ctgccggaac cgtcggcaat ttgaacaatc agctgcttgc gcggtttgaa
     1921 cgttacttcc tgatggataa cctcgccctc gacctgacac ggcacgccaa tcggcgcgtc
     1981 cttaatcggc ataatgtgcg tctcgtcctc gtaacgcagc ggcaggtgca acaccaaatc
     2041 ccacgcggta tggaggttga gtttgtcgag cttcttggcg gaaacatcgg tgattttgag
     2101 ctgtttctgg gtttcgggcg acatcatagg cagattcctt tggacgcgcc tattttatcc
     2161 gaaaacaaaa atgccgtctg aaacggattc agacggcatc ggcaggcagg aatcaagccc
     2221 cggcggcttc ggcttcctgc tgttgttggt aaatcgcttc aaaattaatc ggcgcaagca
     2281 ggacgggcgg gaagccgacg cgcgttaccg taccggaaac agcttcgcgc gcgtaaggga
     2341 agaggatgtt cggacacgcc acgcccaaca gcagctgcac atcttcttcg gggatgtttt
     2401 ccagacggaa aataccgctt tgggttactt cgttcaaaaa catcgtgcgc tcgttatcca
     2461 atttggcggt aacggttacg gtaacgtcca cgtcgtagta gccgtcttcc agcttttggc
     2521 tgccggtgga aacgcgcata tccacttcgg gatcgccctg ttccaaaaag atttgcggcg
     2581 cgtgcggcac ttccaaagac aagtctttga catacagtcg ctcgatgctg aatacgggtt
     2641 gcagttcttc gctcattttg ttttcctagt tgtgggttaa gggttcagca gtccgtccag
     2701 cccgccttcc tgctgaaggc ggtagaggtc ggtaaatcct ccgacgtgcg tttcgccgat
     2761 gaaaatctgc ggcacgctgc gctgtcccga aagccgctgc atttcggcaa aggcttcggg
     2821 gcttgcatcg acacggattt cgtcgatatg tccgacacct gccgcgtgca gcagcctttt
     2881 cgccatcgcg cagtaggggc aaaacggacc tgtgtacatg gtaacggtct gcatattggg
     2941 tttccgaaag ttttgcaatg ataatcaata taggggcaat cgctccgttt ggcaagtgcg
     3001 gaacagatgc acgttcagac ggcatgtgcg gagtgtgtca aagtttcttt tttaaagtat
     3061 gataggcatt gtgaaaaata tttttgcacc cgctctgcgc ggcggggcgg aacggatgca
     3121 aaaatatttt tattacattt tcaggaaaaa ccatgttgtc aggactcccc atccccaaag
     3181 acatcgcgcg cccgcccgaa acgatattgg tcaacatcac gccgcaggaa acgcgcgtgg
     3241 cggtgttgga ggaaaacaat atctgcgagc tgcacatcga gcgcaacagc gggcacagcc
     3301 tggtcggcaa tatctatctg ggcgtggtgc gccgcgtgct gcccgggatg cagagcgcgt
     3361 ttatcgacat cggcttggaa cgcgcggcgt ttttacacat cgtcgatgtc ctcgaacaac
     3421 gccgcaaccc cgaagaaacc cagcgcatcg aacatatgct gtttgaaggg cagtcggttt
     3481 tggtgcaggt catcaaagac ccgatcaaca ccaaaggcgc gcggctttcc acccaaatct
     3541 cgctggcggg gcgtttcctc gtccaccttc cgcaagaaga ccacatcggc gtgtcccaac
     3601 gcatcgaaga cgatgccgaa cgcagcagcc tgcgcgaacg cctcgacaag ctcctgccgg
     3661 aaaatgcctg ccggggctac atcatccgca ccaacgccga aaacgccacc gacgaacagc
     3721 tccagtccga catcgactac ctgaccaaag tgtgggaaca catccaagaa caggcgaaaa
     3781 tccggccgcc ggaaaccctg ctttatcagg atttgccttt aagcctgcgc gtgttgcgcg
     3841 atatggtcgg ctgcgacacg cagaaaatcc tcgtcgattc caccgtaaac cacgggcgca
     3901 tgacgcgttt tgccgaacaa tacgtccacg gcgcattggg caggatagaa ttgttcaaag
     3961 gcgaacgccc gctgtttgaa acccacaaca tcgaacagga aatcagccgc gccctgcaac
     4021 cgcgcgtcaa cctcaacttc ggcagctacc tgattatcga gtccaccgaa gccatgacca
     4081 ccatcgatgt caacaccggc ggcttcgtcg gcgcgcgcaa tttcgacgaa accatcttcc
     4141 gcaccaacct cgaagcctgc cacaccatcg cccgcgaatt aaggctgcgc aaccttggcg
     4201 gcatcatcat catcgacttc atcgatatgg cgcaggaaag ccaccgcgaa gccgtgttgc
     4261 aggagcttgc caaagccctc gccttcgacc gtacccgcgt taccctgcac ggttttacca
     4321 gcctggggct ggtcgagctg acgcgcaaac gctcgcgcga aaacttaaac caagtcctct
     4381 gcgaaccctg cccttcctgc caaggcaggg gccgcctgaa aacgccgcaa accgtgtgct
     4441 acgaaatcca gcgcgaaatc gtccgcgaag cgcgccgtta cgatgccgaa agtttccgca
     4501 tcctcgccgc ccccaacgtc atcgatttgt ttttggacga agaatcgcaa tccttggcaa
     4561 tgctgataga tttcatcggc aaaccgattt ctttggcggt cgaaaccgct tacacgcagg
     4621 aacagtacga tattgtgctg atgtaaaaaa tgccgtctga agccttcaga cggcatctgt
     4681 ctatttcagg gtttccttgt ccaacagcgc gcgtatcaac agcccgtgtc cgaaacgtcg
     4741 gctgtcggtc agttccaaat atccgccgta ttttttggcc agcgtgtcgg caatcgacag
     4801 ccccagcccc gccccctgct gctccgttcc caaaatacgg taaaacggat cgaggacgcg
     4861 ggcgcattcg gattcgggaa tgccgttccc gttatcctcc acccacacgg cgagatattt
     4921 cccttcgtcc gtgaaaccca aatctatcct gcccccgttc ggcgtgtaac gtaccgcgtt
     4981 gtcggcaaag gttttaacca gcgtatagat ttccgtttcg tcggcagaca cttcgacatc
     5041 gcctccgacc gccacaccga tgtcctgacg tttttccaaa accagcggca tcagttcctg
     5101 caacacttgg cggaaacggc tttgcagacc gaatgtcgtt ttcgccaagg ggggtttcgt
     5161 ccgactgcga acgcgccagt gccaaaagct gttcgagcag gtgtttgttg cgcctgatgc
     5221 tctgctgcaa aacggcggac tgccgccccg catcgggtgg gagcggcata ttgttgagcc
     5281 gttccgcctg aagggaaagg gcagtcatcg gcgtacgcaa ttcgtgtgcc gcgtcggcga
     5341 caaaccgctg acggcggcgt atatcttcat caacacgttt caaaagcaga ttgatggcgg
     5401 ttacgaaccc tctgatttca ctgggaatat tgtacacact caaagcaggc aggccgttga
     5461 ttcggcgttg ttcgagactt tgcgacaatt tgcggacggg gcgcatggct ttgtgcgtaa
     5521 tccacacagt cagcagtatc atcagcggca gtgccgccaa caggggcaac acgctttgcc
     5581 gtgccgcatc ctccgccaaa tcttcacggt attcgttttc ctgcatgacg gcaatccgtc
     5641 cctgttcggt cgtgcggata taggcgcggt aataatcgtc gtccccgtcc gcccgaagcg
     5701 tgtgcagacc gtccgccaga tgcgcgggca ggctgacaac agggtcttcc tgccgcggca
     5761 tctgtaccaa aatacgcgta tcgccgtcgc cctcgggcaa agtttcgggc ctggaatcgg
     5821 ggccgacata caatgccgcc tgacggagca ggtcgtcctg caactcttcc gtttcgtgga
     5881 aggtttcgta gtaggaaaac atacctgcaa gcattgccag cggaacaaac atccaaatca
     5941 gggcaaggct gattctgacc tgaagcgaat gttttaaaat tttaaaaaaa cggtccggca
     6001 tcttcgttcc tgttcagacg gcatcttgac gcggcatcag ccagccgaca ccgcgcacat
     6061 tttgtatgct ttccttaccc aatttcttgc acagcccgtg aatcagaaaa tccaccgcat
     6121 tgctttcgac ttccccgccc caaccgtaaa ccttgtcctc cgaatccgag cgcgacaaaa
     6181 tcacacccgg cctcgccagc aaagcctgca ataccgaaaa ctcctggttg ctcaatgcca
     6241 cctgcctccc ctcggcaata atttctacct gatacgttgc aggattgagg gataacgcac
     6301 catttgacag acacgcctgc gcctgtccgc tcccgcgccg caacaccgcc cgcatccgcg
     6361 ccttaaactc cgccatatcg aacggcttga cgatatagtc atccgcaccg ccgtccaaac
     6421 cgttcagccg gctgtacaaa tcatcccgcg ccgtaacgat cagcacgggg acggtacagc
     6481 ctgccgcgcg tatttccgac aaaacatcca aaccgtcccg cccaggcaaa cccaaatcca
     6541 gcagcatcaa gtcataaggc tgggcagcgg cagccgccgc aacctgcgcg ccgtttttga
     6601 cccaatccac cgcatagccg ccgtctttca aacttgccga caccgcttac gcaatcatcg
     6661 cattgtcttc caccagcaaa acacgcatca actttccctt caaaataaac cgtgcctatt
     6721 ctaacacccc aaaattagcc gcaatttagc ggcctttacg cttgccggta tttttcaaaa
     6781 ctgcggcaca aaaaaaccgc gccggcaact gccttcagac ggcattgggg cgcgattgca
     6841 acacacggac aggcaggcaa agcctgcgac agatcacagg aacgattcgg gcttcaggcg
     6901 gcttcgccgt ttacggcaga ggcacgattc ctgccgctat cgaactgatc aatatcgcca
     6961 gcgacaaacc ccacgcccag aaaaacgaat aacggatgtg tttgcccatc gacaatttcg
     7021 ccaaacccaa acccatccac aaagccggcg aaagcggcgt aacaaaagta ccgacgatac
     7081 tgccgatcaa catcgcatag cctgccgctt cgggtgcaac gcccgcctgc gaggtaatct
     7141 gttccacaat cggaaacagt ccgaaataat aagcgtccgt actcaaaacc aactcaagcg
     7201 gaatacccaa cacaccgatg gcaatatgca gataaggcag caacgcgtcc ggcaggatat
     7261 gcacaaggtc tttggaaatc gcgtccaaca tccccgcgcc cttcaaaatc cccaaaaacg
     7321 tacctgccgc caaaataatg gacgccatca tcaccgcgcc gccggcgtgg gcataaatcc
     7381 gctccatctg ttcctgcggg ctgcggtaat tcaaaagcaa cgccgccgtt gcagccagca
     7441 taaatacata acccggcggg aagatgcccg aaaaaagcag gctcatcgcc gccaaaaaca
     7501 gcaggacatt ccaccaaaac agtttcggac gcgccaattt ttgttcttct tccgacaaag
     7561 gcgccggctt tatcaaatcc gccacggcgg gcaacgcgcc caactcccgg acaatccgcc
     7621 ttttttcacg cacacccaaa aacagggaca gcacaaggat aaacaccaca ccgataattt
     7681 gcaccgtcaa caaaggttta tacaattcgc ccacatctgc gcccaacacg cttgcaaccc
     7741 gcccgatcgg cccgccccgc ggcaaaaggt tgattagccc cgcgctggaa gtcagcagca
     7801 aaaacagcag gtaaggattc atatgcagac gcttgtaaag cggcaaaagg gcggggacga
     7861 ccgataaaaa cgtcgtcgcg cccgccccgt ccaactgtgc caccaccgac accaagaccg
     7921 tccccacact cactgccacg atattacccc gagtcagctt aatcaaaccg cctatcatcg
     7981 gacggaacag ccccacatcg ttcatgattc caaaaaacaa aatggaaaac ataaacataa
     8041 tcacaatctg cgtcaccgat ttcgtgccgc ccgaataaaa ttcttttaat tgggatacat
     8101 caaaccccgc cagcaacgcc ccaatcagcg gcaccaagat taatgcgatg atgggcgaca
     8161 ctttttccgt cagcaacagc catacgatga ccccgataat cagcaatccg ataaacgtca
     8221 gcattatttc tcctttattt tattttgaac ataaaaccgc ccgtgcaggc aaaaccgcca
     8281 acatatgcgg gataagcctt gcattccatt cacgcctgcc gacatttctg catccgtccg
     8341 tcatttcacg gcggcaaccg atacggaaca accggcaaac cggtatcggg acggcgcggg
     8401 ggcatccgtc ccggtgcacc gattcaaacg aaactgcccc ctatcattgc ggagcgcggg
     8461 gcgtgccgta cacgcgggat tttaaacacg ccgatcattt gccgcgcatt atccggcaaa
     8521 cggcaaacct tgacgctgcc cggcccatat aaaaaagccg caaaacccga accggttttg
     8581 cggcttttcg atttggtttg tttatttggc atttctttca atcaaaccga caaactgatt
     8641 gatatatgac tgaacaaaat cccttgccga gtcaatcaac ttaccgtttt catcaaacag
     8701 cgtcggcgaa ttgcccaaaa atacttccgg ctgtccggtt acgggcatat caaaatacga
     8761 cagggcaagg cgcaggtttt tttgggaact gtaaccgccc atcttgccga cggaatggct
     8821 gatgatgccg gccggtttgt ttttccaagc cacgtcggca ttcggtttcg agccgatgtc
     8881 caccgcattt ttcaaacagg cgggaatggt gcggttgttt tcggacgtaa cgaacaaaat
     8941 gccgtccgaa gccttaatcg tttcgcggaa agccgtgtag ctttcgggca gcggcacatc
     9001 ttccaccgca gggtcgtcat aatcgaaatt gtaaagcggc agatgtccga tttcgacgat
     9061 ttccgcctgc cagccttcgg ggaacatctc cgccgcattc aatgccactt tgcgcgcaaa
     9121 cgaagcacgg cgcaggctgc ccaccaaaat actgattttc ttagccacaa tcattcctcc
     9181 tgaatattaa gtttgtgcgt ttcaatcatt tcataatggt aacgattatt atatatgtga
     9241 tttcccctgc aaacaagccg gtccgccgcc ccggcgttcc cacttatccg gctttgcctt
     9301 ataattgctt tttttatgta acagattcac ctatgaattt ccccaaaaca gcggcctccc
     9361 tgctgctgct tctcgcctcc ctcgccgcac acgcgctcga taccggccgc attccgcaaa
     9421 acgaaatcgc tgtatatgtc caagagcttg acagcggaaa agtcatcatt gaccaccgtg
     9481 ccggcatacc cgtcaatccc gcgtccacga tgaagctcgt taccgcgttt gccgccttca
     9541 aaaccttcgg cagcaattac cgctgggcga ccgagtttaa aagcaacggt acggtaaacg
     9601 acggcacgct tgacggaaac ctgtattggg cgggcagcgg cgaccccgtt ttcaatcagg
     9661 aaaacctgct tgccgtccaa cgccagttgc gcgacaaagg catccgcaat atcacggggc
     9721 gcctgatgct cgaccacagc ctgtggggcg aagtcggcag tcccgaccat tttgaagccg
     9781 acagcggttc gccgtttatg acgcccccaa atccgactat gctgtctgcc ggtatggtta
     9841 tggtgcgcgc cgaacgcaat gccgccggca gtaccgacat cctcaccgat ccgcctttgc
     9901 cgcatatttt tgcccaaaac aacttgaaaa ttaccgcctc ccaagctgcc tgcccttcgg
     9961 tcaaaaaact gatgcgcgca tctttttcgg gcaatacgct gaaattgcgc ggcaatattc
    10021 ccgaaagctg tttgggcaag cctgtcggtg tccggatgtt cgcgcttgac gaactgatcc
    10081 gccaaagttt taccaaccgc tggctgctcg gcggcggacg gatttcagac ggcatcggca
    10141 tagccgacac accggaaggc gcgcagacgc ttgccgttgc acactccaaa ccgatgaagg
    10201 aaattttgac ggacatgaac aagcgttcgg acaatctgat tgcgcgttcc gtcttcctca
    10261 aactcggcgg cgacggcaaa ctgcccgccg tttccgaaca ggcggcgtct gccgtccggc
    10321 gagaacttgc cgtatcgggc atcgatgttg cggatttggt tttggaaaac ggttcgggtc
    10381 tgtccagaaa agaaagggta acggcgagaa tgatggcgca aatgttggaa acggcttatt
    10441 tcagcccgtt tgcacaagat ttcatcgaca cgctgcccat cgccggcaca gacgggactt
    10501 tacgcaaccg cttcaaacaa agcggcgggc tgttgcgctt aaaaaccggc acgctcaaca
    10561 atgtccgcgc ccttgcaggt tattggctgg gcgacaaacc gatggcggtg gtcgtcatca
    10621 tcaacagcgg ccgcgccgtt tccctgctgc ccgacttgga caacttcgtt gccaaaaaca
    10681 tcatctccgg cggcgacggc tggctggatg cgaaactgat gtgcaaagaa cgccgcgcct
    10741 gaatcgggaa aatataaaaa tgccgtctga actgttcgga cggcattttt gattttcaaa
    10801 ccggaattac agccccgctg ccgctttcaa tgaggccgct ttgtcggtgc gctcccaagt
    10861 aaactcaggt tcttcgcggc cgaaatgtcc gtaggcggca gattttccgt aaatcgggcg
    10921 caagaggtcg agcatttgga cgatgccttt gggacgcagg tcgaaatgtt cgcaaaccag
    10981 cgcaatcagt ttttcctcgc tgattttacc cgtaccgaaa gtatcgatgg aaatcgaagt
    11041 cggttcggca acgccgatgg cgtaggaaac ttggatttgg cattgggttg ccaagcctgc
    11101 ggcgacgatg tttttcgcca cataacggca ggcgtaagcg gcggaacggt ccactttgga
    11161 cgggtctttg ccggagaatg cgccgccgcc gtgcggagcc gcgccgccgt aggtatcgac
    11221 aatgattttg cggccggtca aaccgcagtc gccttgcggg ccgccgatga cgaagcggcc
    11281 ggtcgggttg atcaggtatt ttgtttcgtc ggtcagcagt tcgggcggca aaacgggctt
    11341 gataatctgc tcaatcacgg ctttactcag ttcttcatgg ctgatggcag gatcgtgctg
    11401 ggtggacagg acgacggtat caatgcgttt caccttgccg gtttcgctgt cgtaaaccac
    11461 ggtcagttgg gcttttgcgt cagggcgcag ccaaggcagg cggccgtctt tgcgcaattc
    11521 gctttgacgc tgcatcaggc ggtggctgta atagatggca aacggcatca gggtcggggt
    11581 ttcgtcgcag gcatagccga acatcaagcc ttggtcgccc gcgccttggt tcaggtcgat
    11641 gccttcgcct tcgttcacgc cttgggcgat gtcgggggat tgttggtcgt agtacacgcc
    11701 gaccgcgcag ccgttggcgt caaagcccag ctcggaggag ttgtagccga tgcgtttgat
    11761 ggtttcgcgt gcgactttga tgtagtccac ttgggcggtg gtggtgattt cgcccgccaa
    11821 tacgcacaag cctgtgttga ccaaagtttc cgccgcgaca cgcgctttgg ggtcttgcgc
    11881 caagatggca tccaaaatcg catcggatac ttggtcggct actttgtccg gatggccttc
    11941 ggaaaccgat tcggaagtaa acagatattc gctcattgaa attttccttg aaaaacaaac
    12001 catcttcttc agacagcatg ttgtatgaac ataatgtcga cagcgggaaa tatagcaaaa
    12061 tttccctatt cgtgccattc agttgagaaa tattcctatt taaacggcgc tttggaatct
    12121 ctgtccgtac gtttcgtaca ggcaaaaaaa ttcccgcatc aagcgggttt ggattgctcc
    12181 ggcgagccac atcggttttt caaccgtcca ccttaccttt ccgtttgaaa agcaggttgg
    12241 catggaattc ccaactctta atgcagcgcg catcgtagca gaaaaggcat attgccgcaa
    12301 tacttccctt tttcagacgg catgtttcgt ttacaattca ggctgtttcc ccctttgcga
    12361 accgccatgc acatcctgtt gaccgccctg ctcaaatgcc tctccctgct gtcgctttcc
    12421 tgtctgcaca cgctgggaaa ccggctcgga catctggcgt tttacctttt aaaggaagac
    12481 cgcgcgcgca tcgtcgccaa tatgcggcag gcgggtttga accccgacac gcagacggtc
    12541 aaagccgttt ttgcggaaac ggcaaaatgc ggtttggaac ttgcccccgc gtttttcaaa
    12601 aaaccggaag acatcgaaac aatgttcaaa gcggtacacg gctgggaaca cgtgcagcag
    12661 gctttggaca agggcgaagg gctgctgttc atcacgccgc acatcggcag ctacgatttg
    12721 ggcggacgct acatcagcca gcagcttccg ttccacctga ccgccatgta caagccgccg
    12781 aaaatcaaag cgatagacaa aatcatgcag gcgggcaggg tgcgcggcaa aggcaaaacc
    12841 gcgcccaccg gcatacaagg ggtcaaacaa atcatcaagg ccctgcgcgc gggcgaggca
    12901 accatcatcc tgcccgacca cgtcccttct ccgcaggaag gcggcggcgt gtgggcggat
    12961 tttttcggca aacctgcata caccatgaca ctggcggcaa aattggcaca cgtcaaaggc
    13021 gtgaaaaccc tgtttttctg ctgcgaacgc ctgcccgacg gacaaggctt cgtgttgcac
    13081 atccgccccg tccaagggga attgaacggc aacaaagccc acgatgccgc cgtgttcaac
    13141 cgcaataccg aatattggat acgccgtttt ccgacgcagt atctgtttat gtacaaccgc
    13201 tataaaacgc cgtaacgaaa ataaaaatgc cgtctgaaca ggtttcagac ggcatcttgt
    13261 catttgacga tttcggacaa cgaccaacgc ggacggacgt tgaacgcacc gacctccctg
    13321 cccttgccca ggtgcatcgc accggcaaag gcaatcatgg caccgttgtc cgtgcagtat
    13381 gccatcggcg ggaaaaacac gctgactttt tcggacggat gtttcggctt gcctttgggg
    13441 gtcgggattt gtaccgtcat gttgccgaaa gtttcacgga gtttgcggtt tgcaccgacc
    13501 ccgccggcga ccactacggt tctgaaccct gtctgcaaca gggctttttt cacttttgcc
    13561 tccaacactt ctactaccgc atcttgaaac gcacggcaaa tattgttgcg ggtttgctcg
    13621 ggtatggttt ccgaaccgtt tgcctcacgc actttttcaa cggcagtcaa aacagcggtt
    13681 ttcaatcccg aaaaactcat ctgcaaatcg tcggaatgaa tcatcgggcg cggaaaaaca
    13741 aacgcttcgg gcctgcccga ttccgcaagt tccgacagtt tcgcaccgcc cggatacggc
    13801 aaacccagca gtttcgccgt tttgtcgaat gcctcgcccg ccgcatcatc gacgctctcg
    13861 cccaaaagct cgtaatcgcc tatgcccctg accgccataa tctgcgtatg cccgcccgaa
    13921 accaacagcg cgacaaaagg aaagtcgggt ttttcctccg ccaacagcgg cgacagcaga
    13981 tgtccttcca aatgatggac ggggatgacg ggcttatcca acgctaaagc cagcgcgttg
    14041 gcgtagctcg aacccgccag cagcgcaccg cccaaaccgg gcccctgcgt aaaggcaacc
    14101 gcgtcaatat cgccatacga tgcgcctgcc tgcgccaaac agccttcagt caacggaaca
    14161 aggcggcgga tatggtcgcg gctcgccaat tccggcacga ccccgccgta ttccgcgtgc
    14221 attgccattt gagtgtgcag gcagtgcgac cgcaatcctc gttcggtgtc gtacagcgca
    14281 acgccggttt cgtcgcaaga agattcgatt cctaatacca acatggtctg atgccgttaa
    14341 aaactgaaaa acgtattcta gcgaatttcg gcacgactgc cgtatcccca aaacggaaca
    14401 tgccgtctga agcccttcag acggcatcgt cgcaccgtat caaagcgttc cgtaagaatg
    14461 cagcccgctc aaaaacatat tcacgccgat aaaggcgaat gcggttacga acaaaccgat
    14521 aatcgcccac cacgccagca ctttgccgcg ccagcccgcc accagccgca aatgcagcca
    14581 cacggcgtaa ttcaaccaaa cgatgaacgc ccaagtctct ttcggatccc agctccaata
    14641 gcgtccccaa gcgtccgccg cccacaacgc gcccaaaatg gtggcaatgg taaagaacaa
    14701 aaagccgacg gcaatcgcct tatacatcac ctcgtcgatc aatgccgacg gcggcagcca
    14761 cagttttccg cctttttctt ccgcacgcag ggaaaccagt tcggcaatac cgagcatcgc
    14821 ggaaatgcag aacgcgccgt aaccgataaa gtttgccgga acgtggattt tcatccacca
    14881 ggactggagc gcgggaatca gcggctggat ggtatgcgcc tcacgcgaca cgctgtacca
    14941 caacacaaag ccgaccacga ccgccataaa gccgaacacg aagccgccca atttctgcac
    15001 ggcaaattta ccctcgtaat acaaatacat cagcgcggta atgaccaaaa acagaatgaa
    15061 cacttcataa aggttggaca cggggatatg ccctgcatcg ggacggagca gatagctttc
    15121 gtgccaacgt accagcagac cggtaaagcc tgctacggca gacacccatg caaacacggt
    15181 tcccataccc aacagcgtgt tggtcggcac atttttcacg cttgccaaaa ccgcgcccga
    15241 aatataggcg aacagggcga agaagacaaa agcacactgc cacatgatcg ccgattggct
    15301 gctgaggaaa taccgcaaca ggaaaatctc tgccgattta atgttgccgc catacagacc
    15361 gacggcggca taggcaagca atacgcttaa aggaacaaac cagcgcatcg gtttgaaaaa
    15421 ccaacccaaa aacacggcaa taccggcact cgcccacaac atgaccgttt cgtaaatgtc
    15481 catatgcata ccggaacggg tctgcacgaa aaccgtagcc gcaaaaacca gcacggcaaa
    15541 tacccaatcc caaagattca gattgcggat caaagatttc tgaatcagca gctcgtgttc
    15601 cggaagggtt ttatagtgtt cagtcatgat tcaagtcctt gccgagccgt tgcaggctct
    15661 cgacgtgttt tggaaattcc ttctgcaaat cccgttcgct gcgggccgaa gacatagcaa
    15721 aacggatttt gccgtctgaa aacaataccc acgcccgttt ttcgcgcaca taaaacataa
    15781 ataccgtacc caaaaccaac aataccgagc cgagatagac caaaagcgca cccggcgaac
    15841 gggtcatctg caaacctgag gaacgcacct cggaaaaccc gtcaagctgg agcagcatag
    15901 gcgcgggata ttccgtcagc cccgtatagg catccatact gtgcagcagg aaacggttcc
    15961 gcgcttcatc ctgccgccat tcgggcaagc cgtaccggcg tatggtttca tccaaagcag
    16021 cgttcatcac gccgtaaagc atttcgtaga aatagccctg catcttatcc tgctgccctt
    16081 tcgggatatt ggacgtaata aattcgtcca atcccaaata gcctttttgc gcaaagatat
    16141 tcagcgtgtt ttccgcagcc agcatgaatt gttcgcggat ttcggcaggt gcgtctttgg
    16201 ttgcgtcggc aaccagacgt ttgcgccctt ccccatcttt caaaaactca cgcaatgcca
    16261 taaaggtgtc cgctttcaac tgcttgtcca aggggatacg cagccagcgg tattgctgct
    16321 gcaagccgct gcgcgtgccg gtcagccaaa aataatcttt gtcctgcaaa atcggcagca
    16381 tatagttttt atattcgacc gcctgccctg ccgcatcacg gatgcggtac acgatggaag
    16441 ggccgatatt ggtgtatttt ttaccttcct gagtaacggc gcggacatcg ttcagagtgg
    16501 atttcaggct tttttcccgt tccgcaccct cgctcatgtc ctccacattc atagaagtga
    16561 actgatcgaa ctcaagacga tatttgtgtt tgccgatttc caacggaaac tggtgtatgg
    16621 aggttgcctt caacacgaca ggttcgcgcg aagcatccct caaattccac gccttgaatg
    16681 tcaaatccga accgccgtcg gcaaaactcg cctgataaat cgtgatgccg tgcaaggtca
    16741 aaggatggtt cacgcggatg gtgcgctcga gtttctcacc ggttgccttg tccgttactt
    16801 caatatcgct ggcaaaatcg cgcggcatac ccgtattgta aaaatcgata tggaattttt
    16861 tcagtttgac ttcaaaaggc aagtcctgaa ccaacatccc gttgtcggca ttcaggaaaa
    16921 ccacatccgc actttgcccc tcggaaatat tgacgttgcc cctaaatgag agattggacg
    16981 cacccaaaat actttcgggc ttgaaatcct tggcataaac cgcctgattg tccggaacaa
    17041 tccgaccggc cagcataccc agcttcagca gcaggttact gtctatcaac ccgcccaggc
    17101 aaatgacaat caaagcaaca tgggcaaaga tatagcccca tttgttcatc gtgccttttt
    17161 tggcggcaat cagaaccgac ccgtcctcac ggctgacggt ttttccctga aaaccccgca
    17221 cctccagata acgtttggca acttcggggg caatttttac atccaacagc gaagaatggc
    17281 gcatcgccgc cagagatttt tctttaacct tttcccggaa agacttcatt tcgcgccaaa
    17341 acggcggaac gttacggatt aaacacaaac tggtagaaac caccagaaac atcatgataa
    17401 cgacaaacca tgccgaagca tagacatcgt acaaacccaa aaaatcaaaa atccgagtcc
    17461 aaaacggtcc gaatttgacc aaataatccg tctgcggctg gttttgctgt aacaccgtgc
    17521 cgataaccga tgcaataccc agcagactga gcaaagcgac cgcaaagcgc atggagctga
    17581 aaaaagcgaa ccacggacgg gaaagaagtg tgggggatat acgggattta ctcattgtgt
    17641 gtttattccg ccatcaggaa tatgggaaag cagaattggg cagacagaaa acaacgtccc
    17701 gattctactg tcttgatgct ttttatttca agacaatgaa gacagcctgc atcgattcca
    17761 acggttgcga ttgaaaaaac ttatcgcaaa attttcagaa gccgtctgaa aaccttttca
    17821 gacggcttct gaagcagacc cttggatgat taacgcaaac cctggataaa gttagcaact
    17881 gcttttaaat cttcttcgga catacggttt gcaatatctt ccatgatggt atttttacgc
    17941 tgaccggact tgtaggcatt catctgttca acaatatatg cctggtgctg gccgcccaaa
    18001 cgcggataag cctgaatttc gcttccgcct cccggcatac ccgcaccgct cggaccgtgg
    18061 caggacatac acgccggcac ttttttatcg ctcaaaccgc cgcgatagat tttcgcgccc
    18121 aattcgggat tttccttagg attggcttcg ccggatttgg gctgctgttt ggcatagaat
    18181 gcggatacgt tcaaaatatc ctgatcgctc aaattcatta ccaccggttt catcacagct
    18241 gccgaaccgt gggtgcgttt gccgtcgcgg atgccgatag tttgatgata gatgtaagca
    18301 gtatgctgtg ccgccaaacg cggatacatc gcaatgccgc tgttaccgtc tgctgcatgg
    18361 caagccgcac aaaccgttgc ggcaacctgt ttgccttttt ccacgtctgc tttgggagat
    18421 gcggaaaccg caccggcagc caaaacaaag gccaataaag tcaatcgtct catggagtgc
    18481 tcctgattac agcattggat aacgcaacaa tgctcttttt tatattcaaa tatgggattt
    18541 ttgacccgat taaaaccgat gattctgtaa acgtgttatt ctatactaaa tttacattaa
    18601 attaccactg tgtttcacat aaaaccaacc gcatattttt gctgtcggac aaacggcggc
    18661 ggaaaacaag gatatgccca tgaacctttt tcaaaacgcc aaattcttca cgacggtaaa
    18721 ccatctcaaa gacctgccgg acacaccgct cgaaattgcc tttgtcggca ggagcaatgc
    18781 cggaaaatcc agtgccatca ataccctgac caaccatgtc cgtcttgcct acgtttcaaa
    18841 aacaccggga cggacacagc atatcaactt cttcgagctg cagaacggca attttatggt
    18901 cgatttgccc ggctacggtt atgcccaagt ccccgaagcg gtacgcgcac attgggtcaa
    18961 tctgctcggc gactacctcc gccatcggaa acagcttatc gggctggttt tgattatgga
    19021 tgcccgccat cctttaaaag aactcgacat ccgtatgctg gactttttcc acacgaccgg
    19081 cagaccggtt cacatcctgc tgtcaaaagc cgacaaatta tccaaaaacg aacagataaa
    19141 aaccctgtcc caagtcaaaa aactgctcaa accttattcc gacaggcaaa acatcagcgt
    19201 acagctgttt tccagcctga aaaaacaagg tattgacgag gccaaccgaa ctgtcggaag
    19261 ctggtttgac gcagcagatg ccgccgcttc ctctccagag gaaaactgac ctcaattata
    19321 cggaaaccgt attcccccca cttgaccggc cgcaaacatt taaaaaattg ccactgccaa
    19381 atctaaaatg ccgtctgaaa gacttttcag acggcatttt gcggagtctt taaaacaggg
    19441 aatccaactg ctgctgtttg gaatccgtat tgctcggaag caccggcgtt tcctgtacgt
    19501 cctgacgcgt ttcgtcagac cttccctgct gttcgttttc tactgccgct tcatcatctt
    19561 cttttgcccg tcgggaaggt tgcggcgcaa taccgctgtt gtccagcatc aagcccggat
    19621 cggttaccat acgttccttc atatagtatt cgccattgct gctgaccaca ccttcaggca
    19681 ttttcatccc tttgccctgc tttcctttca acgcaaaacg catatagtcc acccaaaccg
    19741 gcaccgcaat cgtaccgccg tagccggcac gccccatact cttaggtttg tcgaagccga
    19801 tatatacggc agtaaccaca tcagggttaa aaccgacaaa ccacgcatct ttattgtcgt
    19861 tggtcgtacc cgttttaccg gcaatatccg ttcttcccaa cgcagctgcc ccccttgccg
    19921 taccgacacg gaccacatcc tgcataatct tatacataat ataggcattg cgcggatcga
    19981 ttgcctgagg cgcattttgc cctgccacca aaggttgcat ttgggcgcgc aacctgccgt
    20041 ctctgtcata aatcttatcg atcacgtgcg aagaaaccct atatccgccg ttcgcaaata
    20101 cactatatgc ctccgccact ttcaacggcg tcgtctcgcc cgtacctaaa gccatagaca
    20161 ggcttgccgg cagctcggac ggcctgaagc cgaaacgccg gatatactgt tgcgcgtaac
    20221 cgacaccgat agacatcaaa atacggatgg aaaccatatt cttggaagcc gtcagagcct
    20281 gtctcaaagt aatgtagccg gaatatctgc cgtctgaatt tttaggtgtc caaaccgaac
    20341 cgttcggccc tttccccggc agggaaatcg gcgcatcgtt aaccactgtg gacgcggtca
    20401 tccccttaga taatgccgcc gaatagacaa acggcttaaa ggtcgaaccc ggctgccgca
    20461 ttgcctgaac ggcacgattg aatgttttgc tgtgaaaatc ataaccgccg accagcgcgc
    20521 gcacagctcc ggtttttgca tccagcgaaa ccaaagcccc ctgcggcaac ggctcttgaa
    20581 ccaccgccca acgcccgccg ttgtttttga cacggatgac cgcgcccctg cggatacggt
    20641 cctcccccat tttctcatta tcgaccgctc gggccgcaaa acccaaggcg cgcctgtcaa
    20701 gcgcaacccg cctgccgccg ggcagctgta tgacgacatt tttcttttta gtaacatcca
    20761 acacaacggc gggaaccatt ttatcgacgg tatagagtcc cgacagatac tggctgacag
    20821 tctcctcgac atcttcactc ttactcaaat cgatatagtt ttccgcaccg cggtagctgc
    20881 tgccgcgatc gaaattccgt agagccttgc gcaatgcctc ggttgccgcc ttctgatgat
    20941 cggtgcggac cgtggtataa accttaaaac cctgcgtata ggcatcttca ccatatttct
    21001 catacagttc ccgacgcacc atttccgcca catataaagc actctgatcg attttccgaa
    21061 caaaccgctc ataatgcagt tcctcattca atgcctgatc gcgctgttgc acggtaatca
    21121 tcttctcctc gagcatattg ttcaaaatat acttctggcg caacttggca cgctccggat
    21181 taacaatcgg attataggca gacggagcct tgggcagtcc cgcaagcatg gcggcttccg
    21241 ccaaagtcaa atctcggaca ttcttattga aatagatttg cgcggcagat gcaaaaccat
    21301 aggcgcgctg accgaggtaa atctgattga aatacaactc aaggattttg tctttgctta
    21361 aagactgctc gattttatag gcaagcaaca cctcattgaa tttgcgtgtg aacgtttttt
    21421 cactgctcaa ataaaaattt ttcgccacct gctgtgtaat cgtactcgca cccgactgca
    21481 cgctgccgga cacgacattg ccgacggcag cgcgggcaac accccaaaca tccacccccc
    21541 aatgccggta aaagcgttta tcctcggcgg caataaccgc attccgcaac acctcgggga
    21601 aatcgccgat ttttgtaaat tcgcgccgct gctccccata cataccgatg acttctccat
    21661 ccgccgaata aatagtcaac ggcattttag gctggtaatg ctgcaaagaa tccaaagacg
    21721 gcagtttcgg atacgttacc aaaatagcaa tggcaaccag acccactcca aatacacaaa
    21781 aaccaaaaaa caaaccaaaa caagtagtta aaatcttttt aatcatagct gaataataat
    21841 ttaccattat tggtattaaa taaagtaaaa tagcaaccga tttctacaaa gcacggtttc
    21901 aatgtgcaaa gaacaaggaa tccattacgg ataccgaaac ggttactcac tgtacaaata
    21961 aagcaggaac tttcatcatg cgcttgttta aaagcttgaa aaaccctaaa aaaacagatg
    22021 ccaagctccc taaaaaatct tcgggactca ataaccgcgc ggcaatcggc atcgatatcg
    22081 accagcattc catcaaaatg gtacaattgt caggacgtag tttaaaccaa attcaattgg
    22141 aaaaatacgt cattgccaaa ttaccaaaga atatcattca aggcaataaa gtccaaaatt
    22201 acgatcaact tgttacatat ttgcaacaag cctatgccaa actgggtact tcgtgcaaaa
    22261 acatcgtcgc gtccgtcccg caaaatctgg caaccatcga acaattgacc tacacggcca
    22321 aagatgcaga attggacctg caggggttcg tggagtcctc catctccgaa gccagctcga
    22381 tatcgctcga agaagccaat tacgactatc aggtcttgtc ccaatcggcc gtcggcgaag
    22441 ctgttttgtc cgtcgcatcg agaaaggatg aaatcgaacc cctgattgac gcattcaacg
    22501 cagccggtat gaaattatcc gcgcttgatg tggacatttt cggacaatac aacgcctacg
    22561 cgctgtggat aaaccatttt gcccccgagc ttgcggacga aaaagtggcc attttcggcg
    22621 tatatgccgc acagacctac gccctggtca tccaagacgg aaaaatccta tacaaacagg
    22681 aaacctccgt cagcgaagaa cagctcaacc aactcatcca gcgcacctat caggtaacag
    22741 cagaaaaagc ggaagagatc atcaactccc cgcaaaaacc ttccgattac caagaaagcg
    22801 tggcaaacta tttcaaccag cagattaccc aagaaataca aagggtcttg cagttttatt
    22861 acaccacgca gaccgcagac gatatgaccg acatcaagca tatcctgctg acgggggaag
    22921 cggtgcgcca gaaaggcatc gcccaaaccg tcgcctcgca aaccaatgcg gatgtacaat
    22981 gcgtccatcc cgcgcgttat tttgcgaacg acctcaaaac agacgaacaa caattcgaac
    23041 ttgatgcgcc gacactgacc aaggcgttcg gtttggcggt acggggatta taattatgaa
    23101 caatttaatc aaaatcaacc tcctccccta cagggaagag atgaacaaac gcaaacagca
    23161 gcagtttaaa acgctgatgt acggcgccgt gctgacgggc gttgccgccg ttgcggcaac
    23221 ctacctgttt atcgacaata tgatcaataa acagtcggaa aaaaacacgc tgctggaaat
    23281 ctccatcgca cacttggata ccgagctgtc gaaaatacaa aagctcaaac aggaaaaaga
    23341 tgccttcctg attaagaaaa acaaaatcgg ggagctccag atcaaacgcc tccaagccgc
    23401 aaaaatcctc gacagcctga atgaggccgt ccccggaagc acctacctaa cctcgctgga
    23461 tgccgttacc gccgactctt atcggctcag cggcaggaca tccagcgaca accgcgttgc
    23521 cgccatgatg agggcgatgc ccaataccgg catattcaag caacccgaat tgttaagcat
    23581 caagaaaaac aattcgcatc aagaatttac ccttcaggca acattacaac ctatcgtaaa
    23641 ggcggccgaa tccaaagaga atccggcttc gggaaacgca caggaggcaa actgaatggc
    23701 ttctaaatca tctaaaacca acttggatct caacaacctt cacctgctca accttcctgc
    23761 caggcttttt atcgccctgc tggtcgttgc cgccgtgctg gggctcggtt atgccggatt
    23821 gttcaaaagc cagatggaat cccttgagga atatgaagca aaagaaaccg aactgaaaaa
    23881 cacctacaaa cagaaaagta tcgacgcggc cagcctgaac aaccttaggg acgaacttgc
    23941 ctcaatccgc tctgccttcg atatcatgtt gaaacagctg ccgacagatg cagaaattcc
    24001 caatttggtt caagagcttc atcaggcggg ttcgagcaac ggtctgcgct tggacagcgt
    24061 tatgccccaa cctcccgtag atgacggtcc catcaaaaaa ttaccctatt ccatttccat
    24121 taccggaaat tacgaacaga tcagccaatt tacccgcgat gtcggcagtc tctcccgaat
    24181 cattaccctt gagtcgctga aaatcgccca atctccggaa aacggcggca atcctgacgg
    24241 caagagcagt atcctgaacc tcagcgccat tgccaccacc taccaagcaa aatccgtaga
    24301 agagcttgcc gcagaagcgg cacaaaatgc cgagcaaaaa taacttacgt tagggaaacc
    24361 atgaaacact atgccttact catcagcttt ctggctctct ccgcgtgttc ccaaagttct
    24421 gaagacctaa acgaatggat ggcacaaacg cgacgcgaag ccaaagcaga aatcatacct
    24481 ttccaagcac ctaccctgcc ggttgcgccg gtatacagcc cgccgcagct tacagggccg
    24541 aacgcattcg acttccgccg catggaaacc gccaaaaaag gggaaaatgc ccccgacacc
    24601 aagcgtatta aagaaacgct ggaaaaattc agtttggaaa atatgcgtta tgtcggcatt
    24661 ttgaagtccg gacagaaagt ctccggcttc atcgaggctg aaggttatgt ctacactgtc
    24721 ggtgtcggca actatttggg acaaaactac ggtagaatcg aaagcattac cgacgacagc
    24781 atcatcctga acgagctgat agaagacagc acgggcaact gggtttcccg taaagcagaa
    24841 ctgctgttga attcttccga caaaaacacc gaacaagcgg cagcacctgc cgcagaacaa
    24901 aattaagaag aggattactc cattatgaat accaaactga caaaaatcat ttccggtctc
    24961 tttgtcgcaa ccgccgcctt tcagacggca tcggcaggaa acattacaga catcaaagtt
    25021 tcctccctgc ccaacaaaca gaaaatcgtc aaagtcagct ttgacaaaga gattgtcaac
    25081 ccgaccggct tcgtaacctc ctcaccggcc cgcatcgcct tggactttga acaaaccggc
    25141 atttccatgg atcaacaggt actcgaatat gccgatcctc tgttgagcaa aatcagtgcc
    25201 gcacaaaaca gcagccgtgc gcgtctggtt ctgaatttga acaaaccggg ccaatacaat
    25261 accgaagtac gcgggaacaa agtttggata ttcattaacg aatcggacga taccgtgtcc
    25321 gcccccgccc gcccagccgt aaaagccgcg cctgccgcac cggcaaaaca acaggctgcc
    25381 gcaccgttta ccgagtccgt agtatccgta tccgcaccgt tcagcccggc aaaacaacag
    25441 gcagcggcat cggcaaaaca acaggcggcg acaccggcaa aacaaaccaa tatcgatttc
    25501 cgcaaagacg gcaaaaatgc cggcattatc gaattggcgg cattgggctt tgccgggcag
    25561 cccgacatca gccaacagca cgaccacatc atcgttacgc tgaaaaacca taccctgccg
    25621 accgcgctcc aacgcagttt ggatgtggca gacttcaaaa caccggttca aaaggttacg
    25681 ctgaaacgcc tcaataacga cacccagctg attatcacaa caaccggcaa ctgggaactc
    25741 gtcaacaaat ccgccgcgcc cggatacttt accttccaag tcctgccgaa aaaacaaaac
    25801 ctcgagtcag gcggcgtgaa caatgcgccc aaaaccttca caggccggaa aatctccctt
    25861 gacttccaag atgtcgaaat ccgcaccatc ctgcagattt tggcaaaaga atccgggatg
    25921 aacattgttg ccagcgactc cgtcaacggc aaaatgaccc tctccctcaa agatgtgcct
    25981 tgggatcagg ctttggattt ggttatgcag gcgcgcaacc tcgatatgcg ccagcaaggg
    26041 aacatcgtca acatcgcgcc ccgcgacgag ctgcttgcca aagacaaagc cttcttacag
    26101 gcggaaaaag acattgccga tctgggcgcg ctgtattccc aaaacttcca attgaaatac
    26161 aaaaatgtgg aagaattccg cagcatcctg cgtttggaca atgccgacac gaccggaaac
    26221 cgcaacacgc ttgtcagcgg caggggcagc gtgctgatcg atcccgccac caacaccctg
    26281 attgttaccg atacccgcag cgtcatcgaa aaattccgca aactgattga cgaattggac
    26341 gtacccgcgc aacaagtgat gattgaggcg cgtatcgtcg aagcggcaga cggcttctcg
    26401 cgcgatttgg gcgttaagtt cggcgcgaca ggcaggaaaa aactgaaaaa tgagacgagc
    26461 gcattcggct ggggcgtgaa ctccggcttc gggggcggcg ataaatggga ggcccaaacc
    26521 aaaatcaacc tgccggttgc cgctgccgca aacagcattt cgctggtgcg cgcgatttcc
    26581 tccggcgcgt tgaatttgga attgtccgca tccgagtcgc tttcaaaaac caaaacgctt
    26641 gccaatccgc gcgtgctgac ccaaaaccgc aaagaggcca aaatcgaatc cggttacgaa
    26701 attcctttta ccgtaactac agcctcgggc ggcggcaact ctaccaacac ggaactcaaa
    26761 aaagccgtct tggggctgac cgttacgccg aacatcacgc ccgacggaca aatcatcatg
    26821 accgtcaaaa tcaacaaaga ctcgcctgca caatgtgctt caggcaacaa cacaatccta
    26881 tgtatttcga ccaaaagcct gaatacgcag gctatggttg aaaacggcgg cactttgatt
    26941 gtcggcggta tttatgaaga aaacaacggc aatacgctga ccaaagtccc cctgttgggc
    27001 gacatccccg ttatcggcaa cctctttaaa acacgcggga aaaaaaccga ccgccgcgaa
    27061 ctgctgattt tcattacccc gaggattata gatacggccg gcaacagcct gcgctattga
    27121 tgcgtcaaaa taagggcata tgttttacag catatgccct ttctttatgc tttttgccgc
    27181 gaccgaaatg ccgtcattcc cgcgcaggcg ggaatccaac ttgtccggtt tcggttgttt
    27241 ttcgtctcgt aacttttgag ccgtcattcc cgcgaaatcg gaaatccagt ccgttcagtt
    27301 tcggtcattt ccgataaatt cctgttgctt ttcatttcta gattcccact ttcgtgggaa
    27361 taacggcgga agggataaat cctcgcaatc caaagcctcg tcatttccac aaaaaacagc
    27421 aacccgaaac accccgtcat tcccgagcag gcgggaatct aggtctttaa acttcggttt
    27481 ttttccgata aattcttgcc tcattgaaat tctagattcc cgctttcgcg ggaatgacgg
    27541 gatatgggtt tccgtgcgga cgtgttcgga tttccgcctg cgcgggaatg acgattcggg
    27601 tattactgac gattcatcgg gccgtagggt gggcttcagc ccactaattt caccgcatca
    27661 agggttttgg ggaataggcg taactcggcg gtttcggttt ttggcgaagt tggttggaat
    27721 cggcgggctg aagcacaccc tacagtccac cctacaaccc atcttacgca tccgccttac
    27781 gcatttatcc ttcgcctgcc gatatgccgt cattcccgca caggcgggaa tctagaaccg
    27841 caacgcggca ggaatctgtc ggatacggct gaaaccgaac ggactggatt cccgcctgcg
    27901 cgggaatgac ggctgcagat gcccgacggt ctttatagcg gattaacaaa aatcaggaca
    27961 aggcggcgaa gccgcagaca gtacggatag tacggaaccg gttcgcccgg tgcttcatca
    28021 ccttagggaa tcgttctctt tgagccgggg cggggcaacg ccgtaccggt ttttgttcat
    28081 ccgctatagt attgataaac atattatctt caatatattc aattggataa ttgtttacct
    28141 aagcaaagat aattgccttt tcctgacaaa ttaagtgaaa tcaacggatt gtcaaaacac
    28201 agcctgaaat aaaaaacctc cctgatttct tttatttttc cttaaaatca gaaaagttcg
    28261 ggatggtcgg gttatttttc caaacgtacc gccgccctgc cgatttcgta taaaatcccg
    28321 ccgtaacccg acaagcccga accctgccgc cccgaaaggc gggggggtca aacattaagg
    28381 aaccgtgatg aaaaacttta acggcaaact catcctcatc ggactgatgg gcgcgggcaa
    28441 aactacgctg ggccggcaaa tggcgcagcg gctggattac cgtttttacg acagcgatca
    28501 cgaaatcgcc gcagcggcgg gcgttcccat ccccaccata tttgaaatgg aaggcgaaca
    28561 gggattccgt tcgcgcgaaa ccgccatact caaaaaactg attgtcctgc cgcatatcgt
    28621 cctgtccacc ggcggcggcg cggtgttaaa agaagaaaac cgcgccctta tccgcaaaag
    28681 cggcacggtc gtttatctgc acgccccgcc cgaaaccctg ctcgaacgca cgcgctgcga
    28741 caacagccgc cctttgctgc aagttgccga tcctttggcg aaattgcgcg aactctacgc
    28801 cgcacgcgac cccgtttacc gccaaaccgc cgactttacc gtagaatcgg caaactgccg
    28861 ggaaaccgtg caaaccctgc tcaaacgctt atcccgataa accggcatat gcgccgctcc
    28921 ccgaaaacca aaccgcaccc cgcccggcgg gccggcggtt caaactttaa ggaacaacaa
    28981 tgaaaacact gaccgtacac acgccttcgc acagctaccc catctttatc ggcaacgggc
    29041 tgctgccgca ggcgggaagc ctgctcaaac cgcatttggg caaacgcgcc gccatcatca
    29101 ccaacgaaac cgtcgccccg ctctacctcg gcacgcttca gacggcattg gatgcggcag
    29161 gcgtatccca tttcagcatc atcctgcccg acggcgaggc gcacaaaaac tggcagacgc
    29221 tcaacctcat ctttgacggg ctgatgcaaa accgcgccga acgtaaaacc acgctgattg
    29281 cgctgggcgg cggcgtgatc ggcgacatgg tcggctttgc cgccgccacc taccagcgcg
    29341 gcgcgccgtt catccaaata ccgaccacgc tgttgagtca ggtcgactca tcggtgggcg
    29401 gaaaaaccgc catcaaccac ccgctcggca aaaatatgat cggcgcgttt taccagccgc
    29461 aggcggtgct tgcagatttg gacacgctgc acaccctgcc cgcccgcgaa ctgtccgccg
    29521 gtatggcgga agtcatcaaa tacggcgcgc tcggcgacat cggctttttt gaatggctgg
    29581 aacagcatat gcccgaactg atggcactcg aacgcgcgcc cctcacccaa gccgtgtacc
    29641 gctgctgcca aatgaaggca gacatcgtcg cccaagacga aaccgaacag ggcatacgcg
    29701 catggctcaa cctcggacac accttcggac acgccgtcga agcggaaatg ggttacggcg
    29761 tatggctgca cggcgaggcc gttgccgccg gctgcgtgtt ggcggcgcgt ttgtccgaac
    29821 aactgggcaa aacctccgcc gcagataccg cgcggctcgc cgccctgctc gaagccgccg
    29881 gactgccgtc cgcaccgccc gtgtttgcct ttgaaaaatg gctggcacat atgagccacg
    29941 acaaaaaagt cagcggcggc atcatgcgct ttatcggtct gaaccggctg ggcgaagccg
    30001 tcatcaccga aattaccgac acggacatcc tccggcgcac cctgcaaccg tatctctgat
    30061 ttcctctgcc gatgtgttgc cgcgcgggtt tgacgcacga tgatgacttt ccatcatcat
    30121 tctccgcgaa agcgggcatc cggttattcc atattgcagg gatttatccg gaataacgac
    30181 aacctgtccg ccgtcatttc cgcgcagatg gaaatttgga aattcaaaat cacaggaatt
    30241 tatccggaat tatagcggat taacaaaaat caggacaagg cggcgaagcc gcaggcagta
    30301 cg
//
LOCUS       DAXDXW010000018        30220 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXDXW010000018 DAXDXW010000000
VERSION     DAXDXW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30220
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.018"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(120..1262)
                     /locus_tag="ACFB64_000699"
     CDS             complement(120..1262)
                     /locus_tag="ACFB64_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC1209105.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQAGSAPAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTVAITAKGK
                     ELLGGKLPENGWDLLFKPEYTRKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
     gene            1567..1830
                     /gene="rpsT"
                     /locus_tag="ACFB64_000700"
     CDS             1567..1830
                     /gene="rpsT"
                     /locus_tag="ACFB64_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC1209106.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            1990..3138
                     /locus_tag="ACFB64_000701"
     CDS             1990..3138
                     /locus_tag="ACFB64_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC1209107.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPTASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPFWYNNSPNYAPSSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAENLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDWDGI"
     gene            3280..3981
                     /locus_tag="ACFB64_000702"
     CDS             3280..3981
                     /locus_tag="ACFB64_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC1209108.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPPEKAELPEQQ"
     gene            4039..5847
                     /gene="aspS"
                     /locus_tag="ACFB64_000703"
     CDS             4039..5847
                     /gene="aspS"
                     /locus_tag="ACFB64_000703"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC1209109.1"
                     /translation="MRTNYCGLISEQYLGQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLGITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDAGNLSNGEDSGLQSPIVKFLSENALKEIIERTAAQ
                     NGDIIFFGADKAKVVNEAVGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTDAPNSVDDKQLRELSLRLRQKAVETKEA"
     gene            5997..6281
                     /locus_tag="ACFB64_000704"
     CDS             5997..6281
                     /locus_tag="ACFB64_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC1209110.1"
                     /translation="MKKLLLAAVVSLNAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVYDVDADDYWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            6448..8340
                     /gene="speA"
                     /locus_tag="ACFB64_000705"
     CDS             6448..8340
                     /gene="speA"
                     /locus_tag="ACFB64_000705"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC1209111.1"
                     /translation="MPILTIREVCNINHWSIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPFLGFFMVGAYQEILGNMHNLFGDTATADVVVREDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            8439..9362
                     /gene="speB"
                     /locus_tag="ACFB64_000706"
     CDS             8439..9362
                     /gene="speB"
                     /locus_tag="ACFB64_000706"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC1209112.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSFGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            9560..11026
                     /locus_tag="ACFB64_000707"
     CDS             9560..11026
                     /locus_tag="ACFB64_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC1209113.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKVMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLSEMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFVMSVVNFLIFSVIGSIWWKVLGYW"
     gene            complement(11230..12306)
                     /locus_tag="ACFB64_000708"
     CDS             complement(11230..12306)
                     /locus_tag="ACFB64_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFC1209114.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWHIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGIRSHGCVRMKS
                     PDALEFAKTIATGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDTASLKKSIGQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEVSETRTPEVPDVHTPEAQAHLNTQSDGTPTAYTEPAADSSPQVESPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            12370..12507
                     /locus_tag="ACFB64_000709"
     CDS             12370..12507
                     /locus_tag="ACFB64_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HFC1209115.1"
                     /translation="MATETNIAEISALDYEGRGVAKVGGKTIFIKGALLDCLMLGCFR
                     L"
     gene            12529..13671
                     /gene="bioF"
                     /locus_tag="ACFB64_000710"
     CDS             12529..13671
                     /gene="bioF"
                     /locus_tag="ACFB64_000710"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC1209116.1"
                     /translation="MKVFRQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSETCIVPYILGGNEATLAK
                     AEYLQGQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            13681..14328
                     /locus_tag="ACFB64_000711"
     CDS             13681..14328
                     /locus_tag="ACFB64_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC1209117.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGMPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRSKFERRICGDKASFEDYQQFPARPFDEIHQELTALFAMIGQDRR
                     TDLIRWTNALFGSGDKIFIPANQHRYWTPRCTVQETDGGHYLFSRFTHWSALWNH"
     gene            14316..15098
                     /gene="bioC"
                     /locus_tag="ACFB64_000712"
     CDS             14316..15098
                     /gene="bioC"
                     /locus_tag="ACFB64_000712"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC1209118.1"
                     /translation="MESLTAINKPRIRQAFQKALSDYDRHALIQQKMTINLIAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVRSRLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARHSAAGRQ"
     gene            15340..16777
                     /locus_tag="ACFB64_000713"
                     /pseudo
     CDS             15340..16777
                     /locus_tag="ACFB64_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            16780..17878
                     /locus_tag="ACFB64_000714"
                     /pseudo
     CDS             16780..17878
                     /locus_tag="ACFB64_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            17890..18425
                     /locus_tag="ACFB64_000715"
                     /pseudo
     CDS             17890..18425
                     /locus_tag="ACFB64_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            18427..19069
                     /locus_tag="ACFB64_000716"
                     /pseudo
     CDS             18427..19069
                     /locus_tag="ACFB64_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
     gene            19090..19449
                     /locus_tag="ACFB64_000717"
     CDS             19090..19449
                     /locus_tag="ACFB64_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC1209119.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            19541..19616
                     /locus_tag="ACFB64_000718"
     tRNA            19541..19616
                     /locus_tag="ACFB64_000718"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19574..19576,aa:Asn,seq:gtt)
     gene            complement(20114..21025)
                     /gene="crgA"
                     /locus_tag="ACFB64_000719"
     CDS             complement(20114..21025)
                     /gene="crgA"
                     /locus_tag="ACFB64_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC1209120.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLEMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLNLTEEGAQYFRRAQRILQEMAAAETEMLAVHEVPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSHFRVVASPEYLAKHGTPQSAEDLANHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSSCGIACLSDFLVDNDITEGKLIPLFAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVKELGKNMNRTNTK"
     gene            21264..21842
                     /locus_tag="ACFB64_000720"
     CDS             21264..21842
                     /locus_tag="ACFB64_000720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC1209121.1"
                     /translation="MNILLLDGGKAFGHSHGGLNHTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYMDEVFTGGHGKLYQSDGRHRVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFIGLS
                     RLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            complement(22050..23435)
                     /gene="pntB"
                     /locus_tag="ACFB64_000721"
     CDS             complement(22050..23435)
                     /gene="pntB"
                     /locus_tag="ACFB64_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC1209122.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKRGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIHKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLAAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     ISSSGSQEIEEYREVKAADVAEMLKGASSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     IEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFDENSVMCFGDAKK
                     TVDGILAELKK"
     gene            complement(23515..23898)
                     /locus_tag="ACFB64_000722"
     CDS             complement(23515..23898)
                     /locus_tag="ACFB64_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC1209123.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRGFLAHTQ
                     GAAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(23902..25440)
                     /locus_tag="ACFB64_000723"
     CDS             complement(23902..25440)
                     /locus_tag="ACFB64_000723"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC1209124.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLKEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFLQ
                     ESGGSGDGCAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVNL
                     TKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSARPQQTPSEKAAPAAK
                     PEPKPVPLWKKLAPAAIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYHVVWNVSHSL
                     HTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLNM
                     FKKG"
     gene            25729..26562
                     /gene="serB"
                     /locus_tag="ACFB64_000724"
     CDS             25729..26562
                     /gene="serB"
                     /locus_tag="ACFB64_000724"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC1209125.1"
                     /translation="MPQALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEKDAALLDSRQIDHAVLPNMAFGELGLIVSDMDSTLITIECIDEIAAGVGLKGKVAE
                     ITERAMRGELDFGQSLRSRVALLAGLDEQILADIYENVLKLSPGAEFLLDECKRHNVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANILEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPILKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            26626..27849
                     /locus_tag="ACFB64_000725"
     CDS             26626..27849
                     /locus_tag="ACFB64_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC1209126.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIRHTAYGYDADGAY
                     ISFREGVAQAFGMRRVAMLTLCGAIAGGGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAAFFRRTAQKFPFIKRDNIKIIPLAVCMFALIGVIAVWFP
                     EILGNGKAGNQLTFGGLTGWQYSLELTAVKWAVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            28010..29590
                     /gene="purH"
                     /locus_tag="ACFB64_000726"
     CDS             28010..29590
                     /gene="purH"
                     /locus_tag="ACFB64_000726"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC1209127.1"
                     /translation="MSVIKRALISLCDKAGAVEFAQTLHKLGVEILSTGGTAKLLAGA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPGCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGQPQIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYNQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLKAGANRFELKRVGGGLLVQTPDIRR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMAVTGIRHFRH"
BASE COUNT         7381 a         7385 c         7911 g         7543 t
ORIGIN      
        1 ctataaatgt ccgcaatctg aaatcccggc cgtgggggcg acggtataaa tatttctatt
       61 tccatccaat ataaaatgcc gtccgaacat cgttcggacg gcatttttaa tgcttcgaat
      121 cagttggtgc cgactttgat tttctgccac aggctgaccg acagtttttt cgcatccgtg
      181 ctcatttgcg gcatgacgaa accgtctttc atatcctgct cgttcgggaa gatggaacgg
      241 gtgttcacca actcggcagg cattttttcg cgcgccggtt tgctggcggg ggcgaaggtt
      301 acggcgatgc cgtttttcgc cgcgatttcg gggtcgagcg tgtagttgat gtatttgtgg
      361 gcgttggcga cgtttttcgc atcggcggga atcagccaag actcaatcca gaagcccata
      421 cctttcggtg tcagcacttc gatgccgacg ttgtttttca cttcttcgga acgcgctttc
      481 gccaagttca aatcgccgcc gttgcctgcc gccaggcaga tgtcgccgcg cgccagctcg
      541 tcgatgatgg acgggctgaa acgtttgacg tccggacgga tagacttcaa cacttccgcc
      601 gccgccttca aatcttcagg attcgaacct ttggggtctt tgcccaagta gttcagcaaa
      661 atcgggaaca tttcgctcgg ggtgtcccac aaggcgatgc cgcaggattt cagcttgcgg
      721 gtgtattcgg gtttgaacag caaatcccag ccgttttcgg gcagcttgcc gcccaaaagc
      781 tctttgccct tcgccgtaat cgcaaccgtg ttcacgccgg agaaataggg gacggcatac
      841 tggttgcccg ggtcggcggt ttccagcatt ttcaagagtt cgggatcgat gtttttatag
      901 ttgggaatca ggtctttgtt gactttttga tacgcgcccg cctcgatttg gcgcggcagg
      961 aaggcgatgc ccggcacaac caaatcgtaa ccggatttgc cggtcagcat tttggcttcc
     1021 agcgtttcgt tgttttcgta caagtcgtaa gtcagcttca gattgttggc ttttttaaag
     1081 tcttcgaccg tactctcgtc aacatagttc gaccagttgt agatgttcag agtatcggtg
     1141 gcagcggctt cggcattggc agcaggtgcg ctgcctgctt gaggctgcac ggcgtttttt
     1201 tcgctgccgc cgcaggctgc cagagacagc gcggccaaaa cggctaatac ggattttttc
     1261 atacgggcag attcctgatg aaagaggttg gaaaaaaaga aaaccccgcg ccccatagac
     1321 accccggcgc aaggtttggg cattgtaaag taaatttgtg caaactcaaa gcgatattgg
     1381 cctgattttc ctaaaaaatt accctgtttc caaaaaaggg gggggaaacg gccgcccgat
     1441 tttgccgttt ttttgcgccg tcagggtgtc cgacgggcgg atagagaaaa aaggcttgca
     1501 tataatgtaa acccccttta aaattgcgcg tttacagaat ttatttttct ttcaggagat
     1561 tccaatatgg caaacagcgc acaagcacgc aaacgtgccc gccagtccgt caaacaacgc
     1621 gcccacaacg ctagcctgcg taccgcattc cgcaccgcag tgaaaaaagt attgaaagca
     1681 gtcgaagcag gcgataaagc tgccgcacaa gcggtttacc aagagtccgt caaagtcatc
     1741 gaccgcatcg ccgacaaagg cgtgtttcat aaaaacaaag cggctcgcca caaaagccgt
     1801 ctgtctgcaa aagtaaaagc actggcttga tttttgcaaa accgccaagg cggttgatac
     1861 gcgataagcg gaaaaccctg aagcccgacg gtttcggggt tttctgtatt tcgggggtaa
     1921 agttcgaaat ggcggaaagg gtgcggtttt ttatccgaat ccgctataaa atgccgtttg
     1981 aaaaccaata tgccgacaat gggggcggag atgaatacac gaaatatgcg ctatattctt
     2041 ttgacaggac tgttgccgac ggcatccgct tttggagaga ccgcgctgca atgcgccgct
     2101 ttgacggaca atgttacgcg tttggcgtgt tacgacagga tttttgcggc acagcttccg
     2161 tcttcggcag ggcaggaagg gcaggagtcg aaagccgtac tcaatctgac ggaaaccgtc
     2221 cgcagcagct tggataaggg cgaggcggtc attgttgttg aaaaaggcgg ggatgcgctt
     2281 cctgccgaca gtgcgggcga aaccgccgat atctatacgc ctttgagcct gatgtacgac
     2341 ttggacaaaa acgatttgcg cgggctgttg ggcgtacgcg aacacaatcc gatgtacctt
     2401 atgccgtttt ggtataacaa ttcgcccaac tatgccccga gttcgccgac gcgcggtacg
     2461 actgtacagg aaaaattcgg acagcagaaa cgtgcggaaa ccaaattgca ggtttcgttc
     2521 aaaagcaaaa ttgccgaaaa tttgtttaaa acccgcgcgg atctgtggtt cggctacacc
     2581 caaagatccg attggcagat ttacaaccaa ggcaggaaat ccgcgccgtt ccgcaatacg
     2641 gattacaaac ctgaaatttt cctgacccag cctgtgaagg cggatttgcc gttcggcggc
     2701 aggctgcgta tgctcggtgc gggttttgtc caccagtcca acggacagag ccgtcccgaa
     2761 tcgcgttcgt ggaacaggat ttatgccatg gcaggcatgg aatggggcaa attgacggtg
     2821 attccgcgcg tgtgggtgcg tgcgttcgat cagagcggcg ataaaaacga caatcccgat
     2881 attgccgact atatggggta tggcgacgtg aagctgcagt accgcctgaa cgacaggcag
     2941 aatgtgtatt ccgtattgcg ctacaacccc aaaacgggct acggcgcgat tgaagccgcc
     3001 tacacgtttc cgattaaggg caaactcaaa ggcgtggtac gcggattcca cggttacggc
     3061 gagagcctga tcgactacaa ccacaagcag aacggtatcg gtatcgggtt gatgttcaac
     3121 gactgggacg gcatctgaat accgtttcaa acggcatatc aagttgccgt gccgtctgaa
     3181 gccgccggcg gtttggcggg gcgggataaa aaattcgttt gaatccgggc aaacgcgtat
     3241 aatgtgcggt ttgtacggac tttgtcggaa tcaagcaaga tgacggaacc tgcggccgaa
     3301 ggcggcaaag ctgccaaggc gttaaaaaaa tatctgatta caggcatttt ggtctggctg
     3361 ccgattgcgg taacggtttg ggtggtttcc tatatcgttt ccgcgtccga ccagcttgtc
     3421 aacctgctgc cgaagcaatg gcggccgcaa tatgttttgg ggtttaatat ccccgggctc
     3481 ggcgttattg ttgccattgc cgtattgttt gtaaccgggt tatttgccgc aaacgtgttg
     3541 ggccggcaga ttcttgccgc gtgggacagc ctgttgggtc ggattccggt tgtcaaatcc
     3601 atctattcga gtgtgaaaaa agtatccgaa tcgctgctgt ccgacagcag ccgttcgttt
     3661 aaaacgccgg tactcgtgcc gtttccccaa tcgggtattt ggacaatcgc attcgtgtcc
     3721 ggtcaggtgt cgaatgcggt taaggccgca ttgccgcagg atggcgatta tctttccgtg
     3781 tatgtcccga ccacgcccaa cccgaccggc ggttactata ttatggtaaa gaaaagcgat
     3841 gtgcgcgaac tcgatatgag cgtggacgaa gcgttgaaat atgtgatttc gctgggtatg
     3901 gtcatccctg acgacctgcc cgtcaaaaca ttggcaggac ctatgccgcc tgaaaaggcg
     3961 gagttgcccg aacaacaata aagccgccgt tcagacggca ttttccgttt tcagtttaaa
     4021 tcaataaaag gtgattttat gcgtaccaac tattgcggcc tgatcagcga gcaatactta
     4081 ggccaaaccg ttaccgtcaa aggctgggta caccgccggc gcgaccacgg cggtgtgatt
     4141 tttatcgacc tgcgcgaccg cgaaggcatc gtccaagtcg tgatcgatcc cgacacgccc
     4201 gaagcgtttg ccgctgccga ttccgcccgt aacgaatacg ttttgggcat taccggccgc
     4261 gtacgcaacc gtcccgaagg cacgaccaac gataaaatga tttccggcaa aatcgagatt
     4321 cttgccaaag aaatcgaagt gttgaacgcc gccgctacgc cgccgttcca aatcgacgac
     4381 gaaaacatca gcgaaaacgt gcgcctgacc aaccgcgtta tcgacctgcg ccgccctgtg
     4441 atgcagcgca acctgcgcct gcgttaccaa gtcgctatgg gcgttcgccg ttatctggac
     4501 gcgcaaggct tcatcgacat cgaaacgccg atgctgaccc gctccactcc ggaaggcgcg
     4561 cgcgactacc tcgtaccgag ccgcgttcat ccgggcgagt ttttcgcgct gccgcaatcg
     4621 ccgcaattat tcaaacaact gttgatggtg gcgggtttcg accgttacta ccaaatcacc
     4681 aagtgcttcc gtgacgaaga cttgcgcgcc gaccgccaac ctgaattcac tcaaatcgac
     4741 ttggaaacct cgttcctgaa cgaggatgaa atcatggata tcactgaagg catggccaaa
     4801 caagtcttca aagatgcttt gaacgtagat ttgggcgact tcccacgcat gccttattct
     4861 gaagccatgt tctactacgg ctctgacaaa ccggacatgc gcatcaactt aaaattcacc
     4921 gagttgaccg acctgatgaa aacggaagaa ttcaaagtct tccgtggcgc agccgacatg
     4981 aaaggcggcc gcgtagtcgc tctgcgcgtg cctaacggcg ccaaattcag ccgcaaagaa
     5041 atcgacgaat acactaaatt tgtcggcatc tacggcgcga aaggtctggc atacatcaaa
     5101 gtaaacgatg cgggcaacct ctccaacggc gaagacagcg gcctgcaatc tccaatcgta
     5161 aaattcttgt ctgaaaacgc cctgaaagaa atcatcgagc gtaccgccgc gcaaaacggc
     5221 gacatcatct tcttcggtgc ggacaaagcc aaagtcgtga acgaagccgt cggcgcattg
     5281 cgtatcaaag tcggcttgga acacggcaaa gacaacggct acttcacaga cgaatggaaa
     5341 cctttgtggg ttgtcgattt cccgatgttc gaatacgacg aagaagccga ccgctacgtt
     5401 gccgtacacc atccgtttac cgctcctaaa gaaggtcatg aagacctgat ggtttccgat
     5461 cctgcaaact gcctggcccg tgcctacgat atggtattga acggctggga aatcggcgga
     5521 ggctctatcc gtatccaccg tgcagacgta caagagaaag tgtttgccgc gctgaaaatc
     5581 agccctgaag agcaacaaga gaaattcggc ttcctcttgg acaacctgaa attcggtgcg
     5641 cctcctcacg gcggccttgc attcggcctc gaccgtctgg taacgctgat gaccggtgcc
     5701 gaatccatcc gcgacgtgat tgccttcccg aaaacacaac gcgcccaatg cctgctgacc
     5761 gatgcgccca acagcgtgga cgataagcag ttgcgtgaat tgagcctgcg tctgcgtcaa
     5821 aaagcagttg aaacaaaaga agcataagga aaacagagcc gtttgacggc tctgtttttt
     5881 cagacggcat ttacgcttct tgacttccct ctaattcaaa cctaattttg ctgtgtttta
     5941 atggcggtat tgaaaaacac atcttgttca atcaaccgat aaaaaaagga ctgaaaatga
     6001 aaaaactgtt attggccgcc gttgtttccc taaatgccgc aaccgcattt gccggcgact
     6061 ctgccgagcg tcagatttac ggcgatcccc attttgaaca aaaccgcaca aaagccgtga
     6121 aaatgttgga acagcgcggt tatcaggttt acgatgtcga tgccgacgac tactggggca
     6181 aacctgtttt ggaagtggaa gcctataaag acggccgcga atacgacatc gtgttgtctt
     6241 accccgacct gaaaatcatc aaagagcagc tcgatcgctg actcctttga tggaaagatg
     6301 aaccaaaatg ccgtctgaag cgttcagacg gcattttgcc tgttcctcat caggtatgag
     6361 gcaggctttt cttattaaaa aaatgacatt tcacgctgat ttgttataat cattcctttt
     6421 caacacgaca gacggagcag gtttattatg cctatcctta ccatccgtga agtgtgcaac
     6481 attaatcatt ggagcatagg ttattatgat gttgacgatt ccggcgaaat catcgtccgc
     6541 cccaatccct cgcaacacaa tcaaactgtt tcactgcaaa aactgactga agccgtgcaa
     6601 caaaaacatc aggcgcgcct gcctgttttg ttttgttttc cgcaaatcct cgaacaccgc
     6661 ctccgcgaca ttaaccgcgc ctttcagacg gcacgggaag agtgcggcta taagggcggt
     6721 tattgtttgg tttaccctat caaggtcaac caacaccgcc gcgtcatcga gtcgcttatg
     6781 tcaagcggac aaccgcatgg tttggaagcc ggttctaaag ccgaactgat ggcggttttg
     6841 gcacacgccg gcacccggca aacattaatc gtctgcaacg gctataaaga ccgtgaatat
     6901 atccgtttcg ccttgatggg cgaaaaactg gggcatcagg tttatttggt gattgagaag
     6961 ctgtccgaaa tacaaatggt attggaagag gcggaaaaac tcggcatcaa gccccgtttg
     7021 ggtgtgcgcg ccagactggc ttcccaaggt tcgggaaaat ggcagtcttc gggtggggaa
     7081 aaatcaaaat tcggcttgtc ggcttcccaa gttttgcaac tggtcgatat tttgaaacaa
     7141 aaaaacaggc tggactgcct gcagcttttg catttccatt tgggctcgca gcttgggaac
     7201 atccgtgatg ttgccacagg tgtacacgaa tcggctcggt tttatgttga gttgcacaaa
     7261 ctgggggtaa atatccgctg ttttgatgta ggtggcgggc ttggcgtgga ttacgaagga
     7321 aaccgcacac aatcggattg ttccgttaat tacagcctga acgaatatgc cgccacagtc
     7381 gtatggggca tcagtcaggc ttgtctcgaa cacgggctgc cgcatccgac aatcattacc
     7441 gagagcgggc gcggcattac cgcacatcac gccgttttgg ttgctaatgt tataggcgtt
     7501 gaacgttaca aaccgcgtcg gctggatgcg ccatcgcccg aagcaccgcg tgtgttgcac
     7561 agtatgtggg aaacttggac ggatatttcc gcctcgcggg aaaaacgttc cttacgcagc
     7621 tggatacacg aagggcagtt tgatcttgcc gatgtgcata atcagtataa tgtcgggctg
     7681 ttgagtttgg cgcaacgtgc gtgggcggag caactgtatt taaatatctg tcatgaagtc
     7741 ggcgaattgt ttaatgaaaa acaccggtct caccgaacca ttattgacga attgcaagaa
     7801 cgttttgccg ataagctgta tgtcaatttc tcactcttcc aatctttgcc cgatgcttgg
     7861 ggcatagatc aacttttccc tgtttgtcct attaccggtt tgaatgaacc gattgcgcgc
     7921 cgcgccgtgt tgttggacat tacctgcgat tcagacggta cgattgacca ctacatcgac
     7981 ggcgacggca tcgccggtac gatgcctatg cctgattatc ccgaagaaga gccgccgttt
     8041 ttaggctttt ttatggtggg ggcatatcag gaaatactcg gcaatatgca caatcttttc
     8101 ggcgacactg ccactgctga tgttgttgta agggaagacg gacaatttac cgtcatcgat
     8161 tacgatgaag gaaacaccgt tgccgatatg ctcgaatacg tttatcaaga tccgaaagag
     8221 ctgatgaaac gctatcgcga acaaatcgaa cattcagacc ttcctgcctc gcaggctatg
     8281 tctttcttaa aagaactcga agcggggctt aatggttata cctatttgga agacgaatag
     8341 acgcatcaag gtatcggata tgtcgtctga agcccgattt tcttactcaa acaccaatca
     8401 tcacgaccga ttgaaaccaa ttacaaggaa tcattacgat gcaatacagc acactggcag
     8461 gacaaaccga caactccctc gtttccaata atttcgggtt tttgcgcctg ccgcttaatt
     8521 ttatgccgta tgaaagccat gccgattggg ttattaccgg cgtgccttat gatatggcgg
     8581 tttcagggcg ttccggcgcg cgtttcggtc ctgaagccat ccggcgcgcc tccgtcaacc
     8641 tcgcttggga gcaccgcagg tttccgtgga catttgatgt gcgcgaacgc ctgaacatta
     8701 ttgattgcgg cgacttggtt ttttcttttg gcgacagcag ggattttgtc gaaaaaatgg
     8761 aagcgcacgc cggcaaatta ctttctttcg gcaaacgctg tttgagtttg ggcggcgacc
     8821 atttcattac cctcccgttg ttgcgcgccc acgcccgcta tttcggcaaa ctcgcactga
     8881 ttcattttga cgcgcacacc gacacctacg acaacggcag cgaatacgac cacggcacga
     8941 tgttttatac cgcccccaag gaaggcctca tcgacccgtc ccgttccgta caaatcggca
     9001 tacgcaccga acacagtaaa aaattgcctt ttactgtgtt gtccgccccc aaagtcaatg
     9061 aagacagtgt tgaagagacc gtccgtaaaa tcaaagaaac cgtcggcaat atgcccgttt
     9121 acctgacttt cgacatagac tgtctcgacc cgtcgttcgc ccccgggacc ggtacgcccg
     9181 tatgcggcgg cttgagcagc gacagggcat taaaaatcct acgtgggctg acggatctcg
     9241 acatcgtcgg tatggatgtt gtagaagttg ccccctctta cgaccaatcc gacattaccg
     9301 ctttggccgg cgccacaatt gccttggaaa tgctttacct tcaaggtgcg aaaaaggact
     9361 gaacgtccgg catcccccgg gttttcgccg tgccgttcaa acggcgtatt cagtctaatg
     9421 aaaattcaaa tactgaaaca aaagttgccc ggagccgcat atcggaaaga cggtgaaata
     9481 tcgtaatata acttataaaa caattagtta aatattattt ttccgatttt tcgggacggt
     9541 cttttttacg gaggtcaata tgatgaaatt gggtttcaaa ccgatacccc tcgccattgc
     9601 cgcagtattg tgcgccctgg ttttggcact gcccgtaccc gacggggtca agcctcaggc
     9661 ttggacgctg ctggctatgt ttgtcggtgt gattgccgcc attatcggca aggttatgcc
     9721 gttgggcgcg ctgtcgatta ttgccgtcgg gttggtcgca gtaaccggcg taaccgccga
     9781 caaaccgggc gcggcgatga gcgatgcgtt gagtgcgttc gccaatccgt tgatttggct
     9841 gattgccatc gcagttatga tttcgcgcgg tttgctcaaa acagggctgg ggatgcgtat
     9901 cggatatttg tttatcgccg tttttggaag aaaaacgctg ggcatcggtt acagtctcgc
     9961 tctttccgaa ctgctgctgg ctcccgttac cccttccaat accgcgcgcg gcggcggcat
    10021 tatacatccg attatgcagt cgattgccgg cagttacggc tccaatcccg caaaaggcac
    10081 agaaggcaag atgggtaaat atttggcttt ggtcaattat cattccaatc ccatttcgtc
    10141 ggctatgttt attactgcaa ctgcccccaa ccctttaatc gtcaacttga ttgccgaaaa
    10201 tttaggcagt agtttccgtc tttcttgggg ggcgtgggcg tgggcaatgg ctgttcccgg
    10261 cgttatcgcc tttttcgtta tgcctttgat tttatatttt ttgtatccgc ctgaaattaa
    10321 agaaacgccc aatgctgttc aatttgccaa agaccgtctg agcgagatgg gtaaaatgtc
    10381 ggcagacgaa atcattatgg cggtcatttt cggtatcttg ctgctgttgt gggcagatgt
    10441 tcccgccctt attaccggca atcacgcttt tagtatcaac gccaccgcca ccgcatttat
    10501 cggattaagc ctgcttttgc tttccggtgt attgacttgg gacgatgttt tgaaagaaaa
    10561 aagcgcgtgg gatacgatta tttggtttgg cgcattgatt atgatggccg catttttaaa
    10621 taaactcgga ctgattaaat ggttctccgg agtgttggcg gaaagtgtcg gcggtttggg
    10681 cgttagcggc acggctgcgg gcgtaatcct cgtgcttgct tatatgtatg cgcattatat
    10741 gtttgccagt actactgcac atattaccgc tatgttcggc gcatttctcg ctgctgccgt
    10801 ttcactgaat gccccggcga tgccgaccgc gctgatgatg gcggccgcat ccaacattat
    10861 gatgaccctc actcattatg cgaccggtac ttcacctgtg attttcggct cgggctacac
    10921 cacaatggga gaatggtgga aggcgggttt tgtcatgagc gtagtcaatt ttctgatttt
    10981 ttccgttatc ggcagcattt ggtggaaagt tctgggatat tggtaaaggg aaaaataaat
    11041 ttccaatctg tgtttatttg attgggcgac tattatcgtg aaatatgccg tctaaagcct
    11101 tcagacggca tatttgtgcg cttggatgtt gcagaaagcg gcagggcggc ggtgtaggaa
    11161 aagccaaaca aaaaccaaac cgcctgtcaa cttctgataa acataagtat taaataatca
    11221 gaaggttatt caattaccta aacgcaaatt cccctgccgt atcacatcta ttgaaaataa
    11281 tacatcaacc ggctcggaag cagcctgatc aggtgattct acttgcggcg atgaatcggc
    11341 agccggttcg gtataggcag tcggcgtgcc gtcggattgg gtatttaaat gcgcttgtgc
    11401 ctcgggcgtg tgtacatcag gcacttcggg cgtgcgtgtc tcggatactt cggcagagtt
    11461 ggtttcctca gtttgttcaa tgacttcagc ttggctgtat gaggaagaac cctgtatcca
    11521 cgccagcgat ttgagcggca tcttcatctt gccgtttttg ccgcaggtca gacagacggc
    11581 cgatccggtg cggtctttga gtacggcatc gactttctcg ggcgcgatgg ttttgccctg
    11641 tgtttttgcc cattgcccga tgcttttttt cagggaggcg gtgtcaaggt tgttcttacc
    11701 gtaagggtcg cggaaggcgg caagccacag gttgcgatcc gcatcttcat tgaggcccgc
    11761 catttgatag atgacggagg cgggcgagcc ggtggcgatg gttttggcaa actcgagcgc
    11821 gtcgggcgat ttcatgcgga cgcagccgtg actccggatg cccgggacgc tggccggtgc
    11881 attggttccg tgtatgccca aaccgagttt cgggtctcct aagcggacaa aaaccggccc
    11941 caaagggttg tccgggccgg cggctatggt ttttacgccg tcgccgcgtt ctttctgtat
    12001 ggattttggg atgtgccaaa cagggttata ggctttcgcc ccgattttat gttcgcctag
    12061 attggtttgt gtcatcgccc gacctactgc aacgggataa accttggtca gtttgccgtc
    12121 ggtgtagagg aacaggcgtt gctgagggat gttaatgaag acatgttgac cttgtgcgac
    12181 gggggagaca tcgggaatga cggtgtttgc gtatgaaaaa ccgcttatca atagtgcggc
    12241 agtgcggcag attgttttat tcatatcaaa atatggtgtg tgtccgatag gttttcggca
    12301 aatcatacct gaaaccgtac caatttgtgc gaaaatatgc gcttcggtac agtgcggacg
    12361 gattgggtaa tggcaacgga aacaaatatc gcggaaattt ccgccttgga ttatgaaggc
    12421 aggggtgtgg caaaggtcgg cggcaaaacg attttcatta aaggggcatt acttgattgt
    12481 ttgatgctgg gttgcttcag gctttaactc aggaatattt acatcataat gaaagttttt
    12541 agacaacagc ttgaacaact cggcgcgcaa aatcaatatc gttcgattcc ggatttgatt
    12601 catcaagggc ggtatattac gcgggaaaac cgcaaaatgc tgaatatgtc gtctaatgat
    12661 tatttggggt tggcatcaga tgaaaactta cgccggtctt ttttgcagca atacggcggt
    12721 aattttccct cttttaccag ttcttcatca cgtttattaa cgggcaactt tcctatttat
    12781 accgatttgg aagagcttgt cgcacaacgt ttccaacggg aaagcgcgtt attgttcaac
    12841 agcggctatc acgccaatct cggtattttg cctgctttga cgacgacgaa aagtttgatt
    12901 ttggcagata aattcgttca cgccagtatg attgacggca tccgtttgag ccggtgtgcg
    12961 tttttccgtt atcgtcataa tgattatgaa catttgaaaa atctgcttga aaaaaacgtc
    13021 ggaaaatttg accgcacttt tatcgttacc gaatctgttt tcagtatgga cggcgatgtg
    13081 gcggatttga aacagcttgt ccaattaaaa aaacagtttc ccaatactta tctttatgtg
    13141 gatgaagccc acgcaatcgg tgtttatggg caaaacggat tggggattgc cgaacgggat
    13201 aatctgattg ccgagattga tttattggtc ggcactttcg gtaaagcctt agcctcggtg
    13261 ggggcgtatg ccgtctgcaa ccaagtattg aaagaatgtt tgattaatca aatgcgccca
    13321 ttgatttttt caaccgcatt gccgccgttt aatgtggctt ggacttattt tatttttgaa
    13381 cgattgccgc aattctcaaa agaaagaagt catcttgagc agttaagcgc atttttacgg
    13441 cgggaagtgg cgcatcggac gcaaataatg ccgagcgaaa cctgtatcgt cccctatatt
    13501 ttaggcggga atgaagccac ccttgccaaa gcggaatatc tgcaaggtca gggttattat
    13561 tgcctgccca tcagaccgcc gacagtacca aaaaacacat ccagaatccg cctgtcttta
    13621 actgccgata tgacaacgga tgaagttcgg cagtttgcgg cgtgcctgta aggatatgat
    13681 atgaaaacaa aattttacaa tcatcaaggc gaacatttaa tcctgtattt tgcaggttgg
    13741 ggaatgccgc ccgatgctgt aaatcatttg attttgccgg aaaatcacga tttattgatt
    13801 tgctatgatt atcaagattt aaatttggat tttgattttt ccgcctatcg gcacatccgt
    13861 ttggtggcgt ggtcaatggg cgtttgggcg gcagagaggg cattgcaagg aataagatta
    13921 aaatccgcaa cggcagtgaa tggcacaggt ttgccttgcg atgataattt cggtatccct
    13981 tgcgccgttt ttaaaggcac attggagaat ctcacagaaa atactcgttc aaagtttgaa
    14041 cgtagaatct gtggcgataa agcatctttt gaagattacc aacaatttcc cgcccgtccg
    14101 tttgacgaaa ttcatcaaga acttaccgca ctttttgcga tgatcgggca agataggcgt
    14161 acagatctta tccgctggac aaacgccttg ttcggatcgg gcgataaaat ttttattcct
    14221 gccaatcagc accgatattg gacaccgcgt tgcaccgttc aagaaacaga cggcgggcat
    14281 tacctgtttt caagatttac ccattggtcg gcactatgga atcactgact gccataaata
    14341 aaccgcgcat tcggcaggct ttccaaaaag cattaagcga ttatgacagg cacgccttaa
    14401 tccaacaaaa aatgacgatt aatctaatcg cgcatttgca agattatttg ccggatatgc
    14461 cattggaaaa cgtgttggaa ttgggctgcg gctcaggaat gttgagtgcc ttgctgcaaa
    14521 aacagatttc agcgaattat tggttattta atgatttgtg cgatgtacga agccgactgg
    14581 ctgaaaaatt gcctcaatcc tttgattttt attgcggcga tgcggaaaac tttccttttc
    14641 aacgacaatt tgacttaatc gcaagcgcat ctgccgtgca atggtttcac caacccgacg
    14701 cttttatcgc ccattgcaaa acaggcttga aaacaaacgg attattggcg gttgcgacct
    14761 ttggcaaaga caatttaaaa gaagtccgcc aaatcacaaa tataggctta aattacccga
    14821 ctttatccca atggcaggct tggttagcca aagattttga gcttttatgg tgtgaggatt
    14881 ttaaggtaat actagacttt gatacgccgt tagatgtact caaacacctt aaatatacag
    14941 gcgttaccgc cacgaaccaa aaaaattgga caagaaaaaa tctcaatgga tttattggcg
    15001 attacttgtc ggcgttcggt atgccgtcgg gcaaagtgcg cctgacttat catccgttat
    15061 tttttatcgc gcgccactct gcggcgggca ggcaataagg cagcttatgg gcaaagttat
    15121 ttttatatcg ggtattgata ctgatgtggg taaaaggtaa tatggcgagc cttatgcaga
    15181 aggcatattg tcaaacgtta aattatggta tgatttaaaa tttacaagtc tatttcagta
    15241 aatcgttaat aataaaagcg gacaatttaa taataaaagc ggataattgg ccgttgcagg
    15301 cggtcagcta tccgctcgtt acggtatcgg gggtttcctt tggaaaactc attaattatc
    15361 ggtctggcac tggcggtatt gctgatgctt ttggttatgc gtgcgaagca gggtaagaaa
    15421 acaccgaagc gcaaatccca aggtgtcggc aatacgcagg ggcagccgcc cggaagcaat
    15481 gattcggact gggtcgatca gacttggtca atcggtttca gacggcacgc aactcgactg
    15541 gtcttggaac gaaagtgccg agaccgcatc cgccgccgta tccgcgcaag aagtcgatcc
    15601 gcttacggag tatcaggttt ataagcaatt cggttatcag ggcaaggctg ccgaatcttt
    15661 ggctgcctat ctggacggca ttccggatgg tgaagcgaaa cctgagaacc ttatccgcga
    15721 gctgatcgat atcaatctcg aagtggggga tgtcgatgtt ttggcagaca atctgcaaaa
    15781 atacggcaga ctgattcctt tcgaactttt ggcaaaatat atcgaacagg cattacagcg
    15841 agattcaaac catttgcgta tccgcgtctt ggcggaagaa ggtttgggat agggcactca
    15901 ggagattgaa aaacgtgcgg aaggcggttc tgcgacggca gcttccgcat cgcccccgcc
    15961 ggatgtcggc ggtaaggctt atggagccga agaaatcaag cgcatcccga ttgtgcgggg
    16021 caaaaaagac gtgtccggaa tcagccaaga gaaaatcggt gcgattgccg gcctggtccg
    16081 cgccgatcaa ggtgcgaaaa tccttaaaga caaagtcagc cacgaaacgg catcgaaaca
    16141 atacgaccgt gccatccaaa cttccgaaaa acctgcaaac ctgattatcg atgcgttgaa
    16201 actcgattac caacacgcgg acatagaccg ttttgccgga catttgtgga aactttacca
    16261 aacgttgggc aactacggca ggcaggttaa agagcggatg ctgggttagg ggtacagcct
    16321 gggttaccat gaagttttcg atgatttgga aaagggggcg aacgatcggc aaatcaaaga
    16381 catcggtatg aggcacgggt atctgccgaa aaatatacag aaattcaaag cgcaacatcg
    16441 ggatttggtg cttcaagatt cttcgttgat caacactggt tcgtctccgg cagacgatgc
    16501 ggttaaagaa gtagagtcgt tgctgatgta tggtcagatt gaagcggcaa tggatgtgtt
    16561 ggagcaggcg gtattgaaat atcccgacga atcccagctt tatattacgt tgatcgatat
    16621 ttatgaacgt actgaagatt gggataggtt ggggcagttt ttaagggtat tgagggaacg
    16681 tgcggacagg cttcctgaag aggtcgttat gctgatgagc cggctgctgc agcgtatgaa
    16741 tcaaaatatt aaaaaaataa aacggtacgg aaaataaaaa tggaagttca actgccgaaa
    16801 attaaaacag tacgcgtaat gttggcgggg atgacggcgc agcaggaatc cgttttcaaa
    16861 atggcattca aaatgcacaa taccacccgt tatgaaacag tatccccttc agacggcagt
    16921 gccgtgcccg atttggtttt ggcggatacc gatgccgagg gcggttttga actttggaaa
    16981 gagcttgccg ggcgttataa ggctataccc gtcgccgtct gttcggagaa agttcccgat
    17041 tctgaagttc cctacctgcc caaaccgatt cggtttgaaa cattgtttcc tatgctccgc
    17101 aagttattgc agggcgagaa tgtttatggg aaatcgttta ttgcacccgc agaccggtcg
    17161 gcgaaaaaca acgggaatgt gcagcgtacg gttacgatac gccagtttaa cccgaataaa
    17221 ggattattgg gggcgttgcg gtttgcggaa aagaacaggc aggacatcgc tatcttgcat
    17281 ggaaataagc cggtccttaa ttgttttccc ctcgatacaa cgggttttgc tgacagaaag
    17341 tgtgtaaaaa ctcgaagaat tgtgcaaaga cgaaaatttg caggtcagtt gcaagactgt
    17401 tcccgataac tcgcaatggc gcgaaaaggc taaagtaggc attatgtcct gtatgtggca
    17461 gttttccatt tggacggcgc aggacaggtt gatttatccg atttttcccg atactccgtt
    17521 tacgttgaaa tcttggccaa acctgacccg gtaggcaaat gtgccggggt cgatacgctt
    17581 gtcggcattt ttgaccaagg catccgtcaa ccttaacgtg ttgtataaag tgatgccttt
    17641 aaacctcaat gatattctga attatcttgc ggcaacctat acaaccgggt ttttgtcggt
    17701 agatttgaaa acggtttcac aacaggcata ctccgatatg gcggataaaa taaatatcgg
    17761 ggccgattct gcttctgata gtgaaatgat gaaaaaagcg gaaaaaatca cgacaccatc
    17821 ccaatcccag tcgcgcggcc ttctgcaaag actgatgaaa aaactgttgg gcagctaaga
    17881 ggcggagaga tgagagaaaa taaaattatt ttcacaggac ctgtcggcgt agggaaaacc
    17941 actgccattg cggctatttc ggacgaagca ctcgtccaga ccgatgcttc cgcatccgac
    18001 atgactttgg ataggaaaag gaatacgaca gtggcgatgg actacgggcc atcagcttgg
    18061 atgaggatac caaagtccat ttatatagta cgcccggtca ggaacggttc aactttatgt
    18121 gggaaatctt aagccaaggc agtatgggtt tggtattgct tttagataat gcccgaacca
    18181 actcgttgaa agatttggaa ttctttttac attcgtttcg agggctgctg gagaaggcat
    18241 ccgtcgttgt cggtattacc aagatggata tacgctctca gcccggtatc gacgtgtatc
    18301 acaaatatct tgcaaaacat aatcttaatg ttccggtttt tgaaattgat gcccgtaagg
    18361 aagatgacgt aaaacaattg gttagtgcaa tgttattttc tattgatccg ggactggagg
    18421 tttaatatgg aatcaacact ttcactacaa gcgaatttat atccctgcct gactcctgcc
    18481 ggtgcatttt atgccgtatc cagcgatgcc cccagtgccg gtaaaacttt gttgcgcagc
    18541 ctgttgaaag cggatgcgga cgaagtggtc agcagtgaga agctgctcgc gggcggacac
    18601 cgccgacatc gataccgctt tgaacctgtt gtaccgtttg caaaaactcg aattcctcta
    18661 tggcgatgaa aacggtcatt cagacggcat caatttgtcg gacgagcaat tgccgttgct
    18721 gatggaacaa ttgtccggca gcggtaaggc attattggtc gatcggaacg gtctgtatct
    18781 tgccaacgcc aatttccatc atgagtcggc ggaagagttg gggttgttgg cggcagaagt
    18841 cgcacagatg gaaaagaaat accggctgct gattaggaac aacctgtata tcaacaataa
    18901 cgcttggggc gtttgcgatc cttccggtca gagcgaattg acatttttcc cattgtatat
    18961 cggttcaacc aaatttattt tggttatcgc cggcattccc gatttgagca aagaggcatt
    19021 tgttactttg gtaaggattt tataccgccg ttacagcaac cgcgtgtaaa acttgggaca
    19081 ggagggatta tgcagcaatt attgatttca atccttgaag atttaaataa tacatctacg
    19141 gatattatcg cgtctgccgt tatctcaacc gacggattgc cgatggcgac aatgcttcct
    19201 tcacatttga attcggacag ggtaggggcg atttctgcca ctttgcttgc tttggggagt
    19261 cgctcggtgc aggaactcgc ctgcggggaa ttggaacaag tgatgattaa aggaaaatca
    19321 ggctatatcc ttttaagtca ggcgggtaaa gatgccgtgt tggtgctggt ggcaaaagaa
    19381 accggcagac ttggtttaat cctattggat gccaaacgtg cggcaaggca tattgcggaa
    19441 gccatataac atataaagat tgcgggcttg cagataagtg caatcgattg tcaatttata
    19501 ttgacacgtt cggtatttct gttttattat tcgcgtttac tccccgatag ctcagtcggt
    19561 agagcgacgg actgttaatc cgcaggtccc tggttcgagc ccaggtcggg gagccaaatt
    19621 ccaaaaccct ctaagtattt tcttagaggg ttttgtttta ccggcggtca gaaacgcatt
    19681 tttgagatga ttgttttgag atggaataaa atctttgcaa aattcctttc gtgatggtta
    19741 tgaaaaataa atttctaaag atttaattgg ttatttttat aattttgaaa tttatcggtt
    19801 ttgtaaaggt ctcgggataa gcaatttcgg tcttcggcgc gtcttttacg ccaaacccat
    19861 aagaattttg agggtaatct tcatcgtttt gcctgaattg ccgacgcaaa gcgatttatc
    19921 ggcggttgtg tttgagcgtt tgattgcagc ttgcccaatc tcaaatattt ctaaatacca
    19981 attgaaattc aatattttat aaaaacactg atttggaacg aacagattta taaaaattcc
    20041 gtgtatcctt gagtaaggga gttgattgaa aagacttgtt tgattgatga tgaagaataa
    20101 gttaattaat gtcttatttg gtattcgttc tattcatatt ttttcccagt tccttcacta
    20161 aaaaatccaa aaatacgcgt aagcggaggt tgacggcttt atcgctgtaa taaacagcat
    20221 taaaggggtg tgttttattg gaggtttgtt cggcgaatag gggaattaac tttccttcag
    20281 tgatgtcgtt gtcaaccaaa aaatctgata agcaagcaat accgcaactt gaaaggcaca
    20341 acgagcgtaa gatttcaccg ctgctggcgg taaagtgcgg tgaaatttta tagggatttc
    20401 cctgcgcatc taaaaccgcc catgtattta gagaaccggg ttctgtgaag cctaaacatt
    20461 gatggttggc aagatcttct gcagattgtg gcgtgccgtg ttttgctaaa tattcaggac
    20521 tggcgactac gcggaagtgg ctgtcaaaca gatggcgtgc acgcagcccg gaatcgtcca
    20581 attctccggc ccgtaaggca atatcgactt tgcgttcaat cagattgata tagccttcgg
    20641 aagaaacgag cgaaagtcgg atatgcggat agcgttcgtt gaattttgct gccagcggcg
    20701 ccagcagatg cagcaccatc ggcatcgcgg aatccacgcg caacacgcct tgcggtactt
    20761 cgtgcactgc cagcatttcg gtttccgccg ctgccatttc ttgcaggatt ctctgcgcgc
    20821 ggcggaaata ttgcgcgcct tcttccgtca gattgagttg ccgcgtggtg cggttgagca
    20881 ggttcacgcc caacttttcc tccagccgtt tgacgatgcg gcttacggca gaatttgcca
    20941 tctccaactg ctccgccgca cggctgaagc tgccgctttc caccacttga acaaatacgg
    21001 tcagttcttc tgaattggtt ttcatcgtgt ttccttttcg gttggaaccc caccctttag
    21061 ggcggcagga tcagacttta tttgggaagg gcgtaaccca ttcccaatca ggacggcaca
    21121 tagggagacg atttatgtgt catcccgtgt gttgaaacat ctgattattc catttgacgc
    21181 aaaagtgttt tcttattttt gcacttttaa atcataaagt aaaacggtac aatacattca
    21241 tcaattcaca aacgaggtaa caaatgaata ttttattatt agacggcggc aaagcgttcg
    21301 gacattctca cggcgggtta aaccacacgc ttcacaaaaa agcgaaagaa gttttgaccg
    21361 cgctcggaca caatgttcaa gaaaccgtga ttgatgccgg ctatgatgtt gaggcggaaa
    21421 tcgaaaaatt cgtttggatg gatgctgtga tttggcagat gccgggctgg tggatgcacg
    21481 agccttggac agtgaaaaaa tatatggacg aagtctttac cggcggacac ggcaaactct
    21541 accaaagcga cggcagacac cgcgtcaatc cgactgaggg ctacggcaca ggcggcttgt
    21601 tgcaaggcaa aaaacatatg ctttcactga cttggaatgc gccgattgaa gcctttaccc
    21661 gcgaaggcga tttctttgaa ggcaaaggcg ttgatgtttt gtatatgcac ttccacaaag
    21721 ccaacgagtt catcggcttg agccgcctgc cgacattctt atgtaacgat gtggttaaaa
    21781 atccgcaagt ggaaaaatac ttggcagatt atcaggcaca cttggaaaaa gtgttcggct
    21841 aattaaaaat ccatcttcaa cacggagatg gattttgttt gtttcgttga ttttgtgtca
    21901 gtttcagatg tgggtgctta tccggacggg tcgtctgaaa atgtttgccc caatgcaaaa
    21961 aaatcactgc aaaccttcac aaacggggct ggtagtgatt ttttcaaatc aaacagattg
    22021 aaaacctgcg ccgaattgtt cagacggcat tattttttca attccgccaa aatcccgtca
    22081 acggttttct tcgcgtcgcc gaaacacatc acgctgtttt cgtcgaagaa cagcgggttt
    22141 tgtacgcctg cgtagccggt gttcatcgag cgtttgaata cgacgacttc ttttgccttc
    22201 catacttcta aaaccggcat acctgcaatc gggctgttcg gatcggtttg ggcggcaggg
    22261 ttgacggtat cgttcgcacc aatcaccaag accacatcgg tttcggggaa gtcgtcgttg
    22321 atttcgtcca tttccaaaac aatgtcgtag gggactttgg cttcggcgag cagtacattc
    22381 atatgaccgg gcaggcggcc ggcgacgggg tggatgccga agcgtacttc gatgccgttt
    22441 ttacgcaaaa gctcggtgat ttcggcaacg gggtattgtg cctgcgccac tgccatgccg
    22501 tagcccgggg tgataatgac gctgcttgcg cctttcagca tttcggcaac atcggcagct
    22561 ttgacttctc ggtattcctc tatctcttgg ctgccggaag acgatatgcc gctgtcgctg
    22621 ccgaaaccgc cggcaatcac ggaaacaaac gaccgattca tggctttgca cataatgtag
    22681 gacagaattg cgccgcttga gccgaccagc gcgccggtaa cgatgagcag gtcgttggag
    22741 agcataaagc ctgccgctgc ggccgcccag ccggagtagg agttgagcat agatacgacc
    22801 acgggcatat ccgcgccgcc gatggaggca accaagtgcc agccgaatgc gagggcgatc
    22861 agggtcataa tcagaaggat gaagccgctg ccgtcaatgc cgacaaatac gagcagcaac
    22921 acaaacgata cggcgagcgc cagtgcgttg agcttgtgtt tggcgggcag ttgcagcggg
    22981 ctgctgctga ttttgccgtt gagtttgccg aatgcggcca gtgagccggt aaaggttacc
    23041 gcgccgatga aaatgcccaa atagacttcg accagatgga tggtgtgcat atcgtgcgaa
    23101 acgttgcccg gctcgatgta gctgttgaag ccgaccaaaa cggctgccaa gccgacgaag
    23161 ctgtgcagaa gtgcaatcag ttcgggcatt tcggtcattt ctactttttt ggctttgtgg
    23221 atgccgattg ccgcgccgat gagcatggcg atgataatcc agcccagtcc gtgggtattg
    23281 tcggaaaaga ccgttacgaa gagggcgacc gccataccgg cgataccgga atagcagccc
    23341 cgtttggcgg tttcctgttt ggacagcccc gccagtgaga agatgaataa aattgcggca
    23401 acgatatacg ccgctgttac gagtcctgaa gacatggaaa ttctccgatt ttcgatgatt
    23461 tgttttcaat gccgtctgaa aaattgacgt tcgtgttttc agacggcatc tgtttcaagc
    23521 agccgcgaca aacagtccga cggtgcaggc aaatccgccc gcccacatca gcgagcgcaa
    23581 tgctgctttg tctgcgatgt agcaccagat aaaggcgagg cggaacagga tgaacaatcc
    23641 ggcaagcgtg ttgacggttg cttgtccggc attgccggtt gcgtgtgccg tcaaaacggc
    23701 ggcggcaaac ggtgcaaagg cttcaaaacc gttttgctgc gcggcgtggg cacgggcggc
    23761 tgcgccttgc gtatgtgcca gaaaaccgcg aggattgtgg ttgtctttaa accggaatcc
    23821 gcccgctttt ttggcatacg ccgcacaaaa aagcggcaat aggcaggcaa tcagaatgca
    23881 ccaataggcg aaagtcatgg cttacccttt cttaaacata ttcagcatac gccgtgtaac
    23941 cgcaaagccg ccgaagatat tgatgccggc aatcaggatg gcaacaaacg acagcagcga
    24001 aacgaagccg ttgccctgac cgatttgcag cagcgcgccg acgacgatga tgccggagat
    24061 ggcgttggtt accgacatca gcggtgtgtg cagcgagtgg ctgacgttcc aaacgacatg
    24121 gtagccgatg acgcaggcga ggacgaagac gataaagtgg ttcaagaatg ctgcgggtgc
    24181 gaccgcgccg acccacagca ccaatacggc ggcgatggcg gcgggcgcga gttttttcca
    24241 caggggaaca ggtttcggct cgggcttggc ggcaggcgcg gctttttcag acggcgtttg
    24301 ctgcggccgg gcggaaacct gaatcggcgg aggcgggaag gtgatttcgc cgtcgcgggt
    24361 aacggtcata ttgcggataa tcacgtcttc gaagtccagc gtgatttcgc cgtctttgtt
    24421 cgggcttaac agcttggtca ggttcaccaa gttggtggcg taaagctggg aagactgtcc
    24481 ggcaaggcgg tttgccatgt cggtgtagcc gatgattttc acgccgttgc cggttacgga
    24541 caattcgccc ggtcgggtga gttcgcagtt gccgcccgtc gccgccaaat cgacgatgac
    24601 ggatccggat ttcatgcttt ccaccatttc tttggtaatc agcttgggag cgggtttgcc
    24661 cggaatggcg gcggtggtga tgatgatgtc cacttctttc gcctgttcgg caaagagctt
    24721 catttcggcg gcgataaatt cgtcgctcat cactttggcg cagccgtctc cgctgccgcc
    24781 cgattcttgc aggaagtcga gtttcaggaa cttaccgccc atcgattcga tttgttccgc
    24841 cacttccaag cgggtatcga acgcgcgcac cactgcgccg agcgaatttg ccgtaccgat
    24901 tgccgccaaa cccgccacac ctgcaccaat caccaaaacc tgcgcaggcg gcactttgcc
    24961 ggcggcagtg atttgaccgg tgaagaaacg gccgaaggcg ttggcggctt caatcacggc
    25021 gcggtagccg ctgatgtttg ccattgaaga caaagcgtcc aaggcctgag cgcgggaaat
    25081 gcggggaacc atgtccatcg ccagcgcgtt gactttcttg gcgcgcaagg cctcgaccaa
    25141 agcctcgttt tggcgcggcc acaggaagct gacgatggtt tgaccttctt tgagcagcgg
    25201 cagctcgcct tcggacggcg cgttgacctt ataaattaaa gggcaggccc aaaccgccgc
    25261 tttgtcggca acggttgcgc ctgctgtttg gtaagcggca tcgtccaaac ttgccgccaa
    25321 acctgcaccg ctttcgacaa cggtttcaaa gcctagtttg cccagcaggg caacggtggc
    25381 gggcgtgcag gcgacgcggg tttcgccgga taatgactcg cgtgggatac cgattttcat
    25441 ctctgaatcc tttttcgggt tgttcatatg tatcgtgggt taaatttaaa tcggtgcagg
    25501 gcggagcaac gccgtaccgg tttaaaaccg attcacttca aatgttaata tattttagat
    25561 aatcccctta taacgaattt tcatcaggct ggcaatagtt gcgcattttc ccgtgttgtc
    25621 cgacacatac tgccgtttgc gtttgaagtc gttttccgat tgcaggcacg cccgccgtaa
    25681 cattctttgc cgtttcacta tataatccgc attttttgag ccgccgttat gccgcaagcc
    25741 ctcgtcctcc aatttccctc cgccgaagcc ctgccttccg acttcccctc acgcctgccc
    25801 gaacctgatt atgctgatga aaagcgtatg cgttttattg ttgaagaagg attttcttta
    25861 agcgaaaaag acgcggcgtt actggacagc cgtcaaatcg accacgccgt tttgcccaat
    25921 atggcgtttg gcgaactcgg gctgattgtc agcgatatgg attcgacgct gattaccatc
    25981 gaatgtatcg atgaaattgc ggcaggtgtg ggtttgaaag gcaaagtagc ggaaattacc
    26041 gagcgcgcca tgcgcggaga gttggatttc ggacagtctt tacgcagccg cgttgcgttg
    26101 ttggcgggat tggacgaaca gattttggcg gacatttatg aaaacgtttt gaagctctcg
    26161 cccggtgcgg aatttttgtt ggacgaatgc aaaaggcaca atgtgaaatt cctgctggtg
    26221 tccggcgggt tcacgttttt taccgaaagg ctgcaacaac ggctcggctt cgaataccaa
    26281 cacgccaata ttttggaaat tgaaaacggc aggttgaccg gccgtctgaa aggcagaatc
    26341 atcgacgcgc aggcaaaggc ggatttattg cgcgaatacc gcagccgcct cggattgcag
    26401 ccgcatcagg tgttggcgat gggcgacggt gcgaacgata ttccgatact caaagaagcg
    26461 ggcataggcg tggcttaccg cgccaaaccg aaagcgcggg ccgccgccga tgcctgtatc
    26521 aacttcggcg gtttggagcg tgtacgcggc ctgttcggat aggcggatag gaaacggatg
    26581 ccgtctgaaa ggttttcaga cggcatttga acggcaggaa cgacagtggg acgcagaaaa
    26641 ctttggtttg ccctggcggc agcaggcgtt atcggcggtt tggtcggcat tgtgctgacg
    26701 gaactgatgc acttcatacg gcatacggca tacggttatg acgcggacgg tgcgtacatc
    26761 tcgttccgcg aaggtgtggc acaggctttc ggtatgcggc gcgttgccat gctgacgctg
    26821 tgcggcgcga tcgcaggcgg cggctggtgg ctgctcaaac gtttcggcaa gccgcaaatc
    26881 gaaatcaaag ccgccttgaa gcagccgttg caggggctgc cgtttctgac gacggttttc
    26941 catgttctgc tgcaaatcat aacggtcgga ctcggttcgc cgctcggacg cgaagtcgcc
    27001 ccgcgtgaaa tgactgccgc gtttgctttt gccggcggca ggcggttggg tttggacgaa
    27061 ggcgaaatgc gcctcttgat tgcctgtgcg tccggagcgg gtttggcggc ggtgtataac
    27121 gtgccgctcg cctccacgct gtttatcctc gaagcgatgt tgggcgtgtg gacgcagcag
    27181 gcggttgccg ccgcattgct gacttccgtc atcgccaccg ccgtcgcgcg catcggctta
    27241 ggcgacgtgc agcagtatca tcccgccaac cttgccgtaa atacttcatt gctttggttt
    27301 tccgccgtca tcggcccgat acttggcgca accgccgcct ttttccggcg caccgcccaa
    27361 aaattcccct tcatcaagcg cgacaatatc aaaatcattc ccttggccgt ctgtatgttt
    27421 gcactcatcg gtgtgattgc cgtttggttt cccgaaattt tgggcaacgg caaagcaggc
    27481 aaccaactga cttttggcgg tttgaccggc tggcaataca gccttgagtt gaccgctgtc
    27541 aaatgggcgg tcgttttgat ggcgctggcg gcaggcgcat acggcggact gattacgccg
    27601 tctatgatgc tcggcagcac catttctttt gcagccgccg ctgcgtggaa cagcgtcttc
    27661 cccgaaatgc cgtctgaaag cgcggcagtc gtcggagcgg cggtgtttct cggcgtatcg
    27721 ctcaaaatgc cgctgaccgc cattgtgttc gtattggaac tgacctacgc gccgctgtcc
    27781 ctgctgctgc ctttgtgcat cggcatggcg ggggcattgg gaacatcggg aaaaatgggc
    27841 ttcaaatgaa tgccgacaaa tgccgtctga aaggtttcca gacggcattt ctttgcaaat
    27901 tgtcaacaat atctgcaaaa acctactgcc aaaaaactga aaatagcgga taataccgcc
    27961 ccgaaaaaga tcccctttca ccatccaacc gtcagaaaag gaagccccaa tgtccgtcat
    28021 caaacgcgcc ctgatcagcc tgtgcgacaa ggcaggcgcg gtcgaatttg cccaaaccct
    28081 gcacaagctc ggtgtcgaaa tcctctccac cggcggcacg gcgaagctgc ttgccggtgc
    28141 gggcgttccc gtgattgaag tcgccgacta taccggtttc cccgaaatgc tcgacggccg
    28201 cgtgaaaacc ctgcatccta aaattcatgg cggcatcctc ggtcggcgcg atttggacga
    28261 acacgtcgcc aagatggaag agcacggcat cggcaacatc gaccttgtgt gtgtcaatct
    28321 ttaccccttc gccgccacca tcgccaagcc cggctgcaca ctggaagacg caattgaaaa
    28381 catcgacatc ggcggcccga ccatggtgcg ttccgccgcg aaaaactgga aacacgtcgc
    28441 catcgttacc gataccgccg acttccccgc cattgccgcc gaactcgaag ccaacaacgg
    28501 cgcgttgagc gacaaaaccc gtttcaacct ctcacgcaaa gcattcagcc acaccgccca
    28561 atacgacggc atgatttcca actacctgac ctccctttca gacgacgttt tgagcggcca
    28621 gccccaaatc ggcgaattcc caagccagtt caaccaaagc tggattaaag tgcaagacat
    28681 gcgctacggc gaaaacccgc atcagcgcgc cgcgttttac cgcgatattg accccgccgc
    28741 aggcagcctt tccgcctaca atcaactgca aggcaaagaa ttgtcttaca acaacatcgc
    28801 cgatgccgat gccgcttggg aagccgtcaa atcctttgac gcgcccgcct gcgtgatcgt
    28861 gaaacacgcc aatccgtgcg gcgtagccgt tgcagccgat accttgaccg cctacaaact
    28921 cgcctacgcc accgacacca ccagcgcgtt cggcggcatc atcgccttca accgcgaagt
    28981 cgacggcgaa accgtcaaac agattaccga caaccaattt atggaagtcc tcatggcgcc
    29041 gaagtttacc gccgaagccc ttgaaatcgc cgccgccaag aaaaacgtgc gcgtattgga
    29101 agtgccgctc aaagcaggtg ccaaccgctt tgaactcaaa cgcgtcggcg gcggactgtt
    29161 ggtacaaact cccgacatcc gccgcatcag ccgcgccgat ttgaaagtcg tctccaaacg
    29221 ccaaccgacc gagcaggaat ggaacgattt gctgtttgtc tggaacgtcg ccaaatacgt
    29281 caaatccaac gccatcgtgt tcggcaaagg cggtcaaacc tacggcatcg gtgcaggcca
    29341 aatgagccgc gtggacagca cccgcatcgc cgcccgcaaa gcgcaagatg ccggtctcga
    29401 cctcaacggc gcatgtgccg cttccgatgc cttcttccca ttccgcgacg gcgtggacgt
    29461 gattgccgaa cagggcatca aagccatcat ccatccggca ggctcgatgc gcgatcagga
    29521 agttttcgac gcggcggacg aacacggcat cgccatggcg gtaaccggca tccgccactt
    29581 ccgccattaa ggcagatgaa taaggcaatg ccgtctgaag ggttttcaga cggcattttg
    29641 cgccatttac cgtaatcctg aaatcccgtc attcccacgc aggcaggaat ctagatgaga
    29701 atccatcctg tcagaaatac ccgaatcgtc agaaataccc gtgtcatcat tcccgataca
    29761 ccgtaatcct gaaacccgtc attcccgcgc agacagaaat ccggatttgt ccgcgcggaa
    29821 acttatgcgc cgccattccc gcgaaggcgg gaatccggac cgttcggttt cggttatttc
    29881 cgataaattc ctgctgcttt tcattcctag attcccactt tcgcgggaat gacgaaggag
    29941 tgggaatccg gtttttgagt tccggccatt tccgacaaat tgctttggca ttggatattt
    30001 cctattttaa tccactatag tggattaact ttaaaccagt acggcgttgc ctcgccttag
    30061 ctcaaagaga acgattccct aaggtgctga agcaccaagt gaatcggttc cgtactattt
    30121 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccgccat atccgatcgc
    30181 cgcaccggaa ttttaaaaaa taaatacatt ctttaacaaa
//
LOCUS       DAXDXW010000019        27430 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXDXW010000019 DAXDXW010000000
VERSION     DAXDXW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27430
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.019"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            530..976
                     /gene="yciA"
                     /locus_tag="ACFB64_000727"
     CDS             530..976
                     /gene="yciA"
                     /locus_tag="ACFB64_000727"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC1209128.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLATP"
     gene            complement(1027..1848)
                     /locus_tag="ACFB64_000728"
     CDS             complement(1027..1848)
                     /locus_tag="ACFB64_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC1209129.1"
                     /translation="MAVLITGASAGFGEAMCRTFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSCRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDNERVAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKTAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1967..2425
                     /locus_tag="ACFB64_000729"
     CDS             1967..2425
                     /locus_tag="ACFB64_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC1209130.1"
                     /translation="MKTQISLAATALALFLSACGNGGAPAQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2568..3704)
                     /gene="lst"
                     /locus_tag="ACFB64_000730"
     CDS             complement(2568..3704)
                     /gene="lst"
                     /locus_tag="ACFB64_000730"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC1209131.1"
                     /translation="MGLKKVCLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGKPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFYLPYGLNKSFNFIPTMAELKVKSMLLPKVRRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTNNLIRESSYLGDEFYVNGAIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISQKAAKNFNIQY
                     VAPHPRQTYGLSGVTALNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFRQADIPILAFDDKNQSHGKSK"
     gene            3840..4010
                     /locus_tag="ACFB64_000731"
     CDS             3840..4010
                     /locus_tag="ACFB64_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix 2"
                     /protein_id="HFC1209132.1"
                     /translation="MPPHIRHNAADAAAKRGKAISPFLSRRRVISPFLSRRRVQTRQD
                     RTKREQSRQNMP"
     gene            4077..6302
                     /locus_tag="ACFB64_000732"
     CDS             4077..6302
                     /locus_tag="ACFB64_000732"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC1209133.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPGANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWAKDSKTHVATMQSGDFFHNEQSVTVPDATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDAAVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLIANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGNVLMQHD
                     VEAGGIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFAPLAAALTADEAKIVEELSAVQGKAVDIGGYYAANPEKAAQAMRPSATFNQVLNA
                     L"
     gene            6599..6901
                     /locus_tag="ACFB64_000733"
     CDS             6599..6901
                     /locus_tag="ACFB64_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209134.1"
                     /translation="MKTVPTILTGILLATALPASAHGMHKSKPLAMDELPPICQQYFK
                     RTETCYNKAGNKADFARNNTKFLFQALPAADLGQRKQMCQIAMDSFAEKTRNLNCE"
     gene            7860..8027
                     /locus_tag="ACFB64_000734"
     CDS             7860..8027
                     /locus_tag="ACFB64_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209135.1"
                     /translation="MVNEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNLDFSQW
                     VKSIIKEKIKK"
     gene            8187..9032
                     /locus_tag="ACFB64_000735"
     CDS             8187..9032
                     /locus_tag="ACFB64_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFC1209136.1"
                     /translation="MNAVQVLNFQQNSVRTVADNKGELWFLANDVCEILGYTNPRRTV
                     DLHCKSRGVTKRYTPTASGEQEMTYINEPNLYRLIIKSRKPAAEAFEEWVMETVLPAI
                     RKTGGYQITPKTTADDRTGLRRAVAALVGRKGIDYSSAYSMIHQRFNVESVEDLPAGK
                     LPEAVAYVHALTLHTGLTGEVLDAPPKAEPKLPIDGNSLADIAAMVYYGTWMIESGKD
                     ISAPLKQLGCRQAVTMWTVWHETRSILKRSAAALEVLRGYADKDASDRIAACLEGIYG
                     KATVR"
     gene            complement(9069..9218)
                     /locus_tag="ACFB64_000736"
     CDS             complement(9069..9218)
                     /locus_tag="ACFB64_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209137.1"
                     /translation="MSAEEFCRKQIAYWLNESRKASDNADLKAFEFAGREPADYREML
                     KRYAA"
     gene            complement(9221..9895)
                     /locus_tag="ACFB64_000737"
     CDS             complement(9221..9895)
                     /locus_tag="ACFB64_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209138.1"
                     /translation="MNLKALIARFRVLANDKAEPYFWSDEEVSGWLNDAVHEACLRGR
                     LLHSDDAFVTDVEKGRPLYAYAAGGFAAGYAYEIDSIRFVSDGKPVCLKLVSPEAADV
                     CAPGWRDGAQTGLPVYAVQGDGKLTLAPAPDRDGRLFAGGYCLPRDMAGDGDEPEINS
                     IHHRNLVYWALAEAFSIPDAETFDPQRSESARRRFELYFGLPADSDLRRITREDAPHL
                     NRHFWI"
     gene            complement(9892..10377)
                     /locus_tag="ACFB64_000738"
     CDS             complement(9892..10377)
                     /locus_tag="ACFB64_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209139.1"
                     /translation="MKRRPGICLCGVAVFDNAVRTAAGGWMDKVFIKYIGGRAVWRDR
                     IYHTGLVFEDGQVREVSAGAAAKLLRHGDVFAAVPGKRVEKADDTEALEKAGASELER
                     ETAAFDAVQDVILQINRMGKDELELYAKANYGQGLDKRKSAENLRGDVVRMVRQFGIA
                     Q"
     gene            complement(10383..10856)
                     /locus_tag="ACFB64_000739"
     CDS             complement(10383..10856)
                     /locus_tag="ACFB64_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209140.1"
                     /translation="MAEIYVKNNGGNRFGGVPYGNLAAEHYRIVAKQDGAILGADASE
                     PPKAGDVLALGVLEQGFRLDDAQIIVKTAMSSGITADVGFAYADGADDAKVPQDAAYF
                     ASGADFASAGRIRCQSAKLVTLPKQALLTVTLKGAENKKAADIDILIYGEKFGQL"
     gene            complement(10911..12206)
                     /locus_tag="ACFB64_000740"
     CDS             complement(10911..12206)
                     /locus_tag="ACFB64_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HFC1209141.1"
                     /translation="MAQKTNTAYGDPQAMMRQAAGLFAMHMQRNSTLNRLAGKMPAGT
                     AGAEATLRKQTTQHMPVVRCQDLTRGMGDEIRFNLVNPVSALPIMGDNTAEGRGVGMS
                     LSEAGLRVNQARFPVDGGGTMTNQRSPADYRALIRPAAQSLMDRYADQTLLVHMAGAR
                     GFHDNIEWGVPLAGDPKFNDYAVNPVKAPSKNRHFTASGDAVTGVGDNGGELKIASTD
                     LFTMDTVDSMRTVLDQIPLPPPIVKFEGDKAAGDSPLRVWLLSPAQYNRFAADPKFRQ
                     LQASAIARASQANQNPLFLGDAGLWNGFILVKMPRPIRFYAGDEMKYCADKFSEAESG
                     LKIPASFADKFAVDRSVILGGQAVLEAFANTGKHGGMPFFWSEKELDHGNRVETLVGT
                     IRGVAKTRFAVDVGGGAKEITDYGVTVVDTVVPLHGGIR"
     gene            complement(12227..12964)
                     /locus_tag="ACFB64_000741"
     CDS             complement(12227..12964)
                     /locus_tag="ACFB64_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209142.1"
                     /translation="MGGIAGGRQYGRGGRRGYRRGGRRADKVIRAKDGKHTIPYEKLV
                     EAREAEKSAKAEAQALRERIAQLEKGAEKPGVETADGSDNSLFGDFSDEDVKKGVEKL
                     IQEKLAGYEADMKRQEAAKAHYREIYTAHPDADSIVESRELEEWLAGQNPLVRKAFND
                     ALKDGTAAEVIGAFDMFKAAKSAAEPEKPAEKPPAGKNTPNTLSDIPAGRDHTASDGP
                     PDYLSGNALAEKLASMTEEQVEKFLNS"
     gene            complement(12946..18741)
                     /locus_tag="ACFB64_000742"
                     /pseudo
     CDS             complement(12946..18741)
                     /locus_tag="ACFB64_000742"
                     /inference="COORDINATES: protein motif:HMM:NF032893.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
     gene            complement(18944..20158)
                     /locus_tag="ACFB64_000743"
     CDS             complement(18944..20158)
                     /locus_tag="ACFB64_000743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209143.1"
                     /translation="MSDLVRYDPLEHGRLVGGLKEYRGFTQKDARAAADDTALTRGFK
                     NSMRSARMGWNALTGDKEELGRLKAEDMDYRKIQEGRKSQARRELGEAWEKGGGVGGG
                     LSNVWGELKKDWREKGLDGALEDVGEMAGAVLEQAPNALVPIATTTAGGILGALAGGN
                     AAVGAYAGATLGNTLMEYGGQLDRAAEAAGVDPADKDAVMAFIGRGAPGALKNAAVKG
                     AVVGAADMAAMKLGGSILNMGKKAAGKAALEKMGVAAADKAAVAAAKGTPEFAALAKE
                     SAKGGLGGAARHAAAYATESAGEFAGEYLGTGLANGEWDEKGAALEAFSSLGHSAVGF
                     AGTKAYAAVTDPLRPPAGRKAGAQGGIGGNRKAGRPAPEGAQALRAAAQAAADGGTGG
                     CGRRRGTGRRGF"
     gene            complement(20155..21351)
                     /locus_tag="ACFB64_000744"
     CDS             complement(20155..21351)
                     /locus_tag="ACFB64_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209144.1"
                     /translation="MGLFEPSAGDFWEMKEKEKKEKARKGAEERERAAAQAHRADAVR
                     RTVANYEAGPARYRNVMDLSRNNIEDGARRLRRAGAFERGADAGLGFSGGDKALSPDA
                     RAGADFARRDTRPTDAGGRTPPPLGFDGNVYRDGKPVRDFDAQRPLVSAGPDALSPEE
                     RELYRRATTPYAGALNGQLTAAQLNAARGIVAEHNKNAAVRELGRERLAAAAAENAAN
                     REAVLQKGRFDAAVKANEGALNREMAQRNADRAFDVQQAELGMKRQGFEMKREADALE
                     LEDRKRIADLTRAYGFAKSDGQRGEIARQIDALNGKFERQGEKGFDPNVFKTISYEVA
                     DPDTGLTAKREGIVDLRTGKPLDVEFAGEREKRYAALGFKPNGQKTAGGKIIYENEKG
                     EKRVEQ"
     gene            complement(21588..23855)
                     /locus_tag="ACFB64_000745"
     CDS             complement(21588..23855)
                     /locus_tag="ACFB64_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209145.1"
                     /translation="MEALLMGTDVPETGVLPDKNGEPLTIGEYRLFVGEMMNQPAWRA
                     VADKEMDYADGRQLDNELLQKQRELGLPPAVENLITPTLLSVQGYEATIRTDWRVTAD
                     GETGGRDVADALNFKLNRAERQSRADKACSDAFRGQIACGIGWVEVTRNPNPFEFPYE
                     CGVIHRNAIHWDMKSYKYDLSDARWLIRRRWLLPERLAQFFPEYAGHFKAMGRGGSDW
                     RISGEMLDGGGNTGLADAWGISGRNTVSEEFWFNETTRELAVAEVWYRRWVTADCLRD
                     KKTGRTVEFDGANPNHREMAANGAVLFAASVPRMRRAFVVGDLVVRDEPTPYPHQKFP
                     YVPFFGFREDNTGIPYGYVRNMKYAQDNLNSTNSKLRWGLSAIRTVRTKGIVDMSDEQ
                     FRRNIARVDADIVLNKIEAAQPGARFDVSRDFELSAQHWQMLQDSRATIRQISGITPS
                     FMGNRGNATSGRQESIQVEQSNQSLGLVMDNFRQSRSLVGELLLAMIIEDLGSDEQTV
                     VIEGDAVTQGRTVVINRPETDPVTGKAYLSNDLQNIRLKVALEDVPSTNSYRSQQLGA
                     MSEAVKSLPPEYQAAVLPFMVSLMDIPFKDKVIEKIKEVRVQETPEQIEARIAQAVQD
                     ALAKSGNDIKRRELALKEQRTASEIKEIEARAVQIGVQAAYAAMQAGGQIAAMPQIAP
                     VADAVMQGAGYIRPARGDDPGFPVPAMPPETQIPPEGIPEAYGADTGPMTAVPPKSAN
                     HAQTGMETPTVSDNL"
     gene            complement(23840..25114)
                     /locus_tag="ACFB64_000746"
     CDS             complement(23840..25114)
                     /locus_tag="ACFB64_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFC1209146.1"
                     /translation="MTGKTVDLKLPAKLDGLFKPCRYKVMYGGRGGGKSHGAASALLA
                     LGAQRPLRILCVREIQKSMRDTVHRLLKDKVAQLGLGHFYEITDFEIRGANGTLFVFS
                     GLQSHTVDSIKSFEGIDIVWVEEGHGVSKKSWDVLTPTIRKEGSEIWITLNPDMETDE
                     TYRRFIAMPSEDTWLCEINWRDNPWFPEALNRERLKAQRSMNKEDYGNIWEGRPRMVS
                     EGAVYRHEIQDAFHSGRVTLVPYDSSLPVHTVWDLGWNDAMTIGLVQRDLTSVRIIGY
                     IEDTHRTLDWYVAELEKLPYRWGTDFLPHDGRTRNFQTGKSTMEILTGLGRKSVFVQN
                     ATGIEEGIRAARMLFPKVYFDKDKTARLLECLKRYGRQIHAKTGVAMGPLHDEYSHGA
                     DMFRYLAQAVDLMDTGSNTGYTETPVSDWRLY"
     gene            complement(25095..25634)
                     /locus_tag="ACFB64_000747"
     CDS             complement(25095..25634)
                     /locus_tag="ACFB64_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209147.1"
                     /translation="MPRLSDEDWRKVELDYRRGVLSIAEIGRKYNVSAQHVGRVAKER
                     VWTRDLNDEVQAKARAMVLSADKNGNAPDYADFNLKQVTDAEAKRVAAVQRRHRNLAE
                     NLSKSAEQIVAELYGSPEDTFTKARMFQTVAAGFKVLVEIERKSYGMDTAESKISESA
                     KTAGIRIEFVGPEDDGKDG"
     gene            complement(25634..26056)
                     /locus_tag="ACFB64_000748"
     CDS             complement(25634..26056)
                     /locus_tag="ACFB64_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209148.1"
                     /translation="MSGLSDIDKVYQGVVDLHNAEQVVSREALAETTGLKMSEITKFT
                     KLLVEHGKIYRVTRGIFKPAIGFGETRPVSVSVLDSGMGVLEIGDTVLHLNPQEMRSL
                     GALMSGFGQQFSSIQMGREFSVLRNYLECSAKNGRLDL"
     gene            complement(26121..26639)
                     /locus_tag="ACFB64_000749"
     CDS             complement(26121..26639)
                     /locus_tag="ACFB64_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC1209149.1"
                     /translation="MGLTQEVLKELLRYDDNTGKLYWAERPRKYFNSGLHYKSWNTGF
                     SGKEVFLYKGRLGYLKLKIFKKQYNAHRLIWLFVYGKHASSIDHINRDKTDNRISNLR
                     DVTHAENMKNRGKFKNNTSGHTGVYFHKPSKKWQARIMVNRKNKILGLFEHIEDAVKA
                     REAASKDFGFVV"
     gene            complement(26630..27013)
                     /locus_tag="ACFB64_000750"
     CDS             complement(26630..27013)
                     /locus_tag="ACFB64_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFC1209150.1"
                     /translation="MNGAEFTLTPQNKKQVMRSIWDSPDGWFENGNLEITIRPRKSKR
                     SVEQNRRLWFLYREISEKVFIDGRRFSQDVWHEFLKRKFIGCIEMPNGQLMGISTTKL
                     SVREMSEYQEKIISWASMEHGVLWD"
     gene            complement(27010..27315)
                     /locus_tag="ACFB64_000751"
     CDS             complement(27010..27315)
                     /locus_tag="ACFB64_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease domain-containing protein"
                     /protein_id="HFC1209151.1"
                     /translation="MSSAIRKAAKGGQCTPNIAGVCNDNPETVVLCRFPGETHGAGLK
                     SGGLGAGFGCGCRRGAIDGRGAGLSREDKEFYMRRSQLRTIRRLEALGVVGVKGRLK"
BASE COUNT         6151 a         9112 c         6312 g         5855 t
ORIGIN      
        1 cgggaatgac ggttcggttg catagggtca gattgtcgaa aggggcggat tcgatggatt
       61 cgatgaaaac ggtagaaatg ttggattgat gggaatggcg gactgaagcc caccgattca
      121 tcgactccaa cgtttacgat gcttccaacg gtttcagacg gcatttttta cacaattccc
      181 gccattttcc atcattcccg acaacaccgc aatctcgaaa cccgtcattc ccgcgcaggc
      241 gggaatctag gatgcggaat ctagagaaac cttttaaccg ataagtttcc gtgcggacag
      301 gtctggattc ccgcctgcgc gggaatgacg gttcggttgc ttagggtagg atttggcggg
      361 attggtgggc tgaagcccgc cctacgcatc cgccctacaa tactccttgc gaatctgtcc
      421 cgcgcccgac cttgtatgtc tcaaatgggt taaactacgc acttcccgac ttcccgtttc
      481 gggcaggggt gcaggtccga tttatatttc aataaaacaa ggaaacttta tgcagcacga
      541 agaaggcaac cgccaacgcc ctcaaggcga actgctcctg cgtaccgtcg ctatgccgcg
      601 cgataccaat cccaaccaag acattttcgg cggctggatt atgtcgcaaa tggatttggg
      661 cggcggcata ttggcggcgg aaatcgcgcg ggggcgcatc gttaccgtcg ccgttcagga
      721 aatgaacttc atccgcccgg tcaaggtcgg caacgtcgtc tgctgctacg ggcattgcgt
      781 ccgcgtgggc aatacttccc tccagcttaa agtcgaagtc tgggtgaaaa ctttgatgaa
      841 cgattgcgtt accgaagacc gccatctcgt aaccgaggcg gtgttcactt atgtcgccat
      901 cgatgcggaa ggcaatccgc gcccgattcc gaaagaaggc aaccccaaat tgtcaggctt
      961 attggccacc ccgtaagaat acccgtaaaa atgccgtctg aaactgtctt cagacggcat
     1021 tccgtatcat ttgaaccaag acctgatgcg ggaaaacagc gacatactct gtttttcaaa
     1081 gtcttcccgc acttcgggca cggctgtttt tatgaccttc attcctgcaa aagtctgcgc
     1141 cacaggcata atttcaatcg tgttcacatt catatgcgcc ggccggcggt acagccacaa
     1201 tgcggtttcc gcaatatctt cggggcggat aaattccaca ccctcataca cgcccgccac
     1261 cctctcgtta tcgcctttga agcgcacgtt ggaaaactcc gtattgccgc acaaacccgg
     1321 ctcgatattg gtaacgcgga tgttcttatc cgccagctct gcgcgcaaat tcaggctaaa
     1381 ctggcgcaca aacgccttgg tcgccccgta aacgttgctg ccggcataag cataattgcc
     1441 tgcaatcgaa cccaaattca tcacataacc gccgccgcgt tccaccattt gcggcaaaat
     1501 cttgcgcgtc aggaacgtca aacccaaaac attggtttga atcattgttt cccaatcttc
     1561 aaaatccgcc ttgtccgccg tgtccaaacc caaagccagc ccggcattgt tgatgaggca
     1621 gtcgatgtcg gaaaattcat cggggatgcc gttcaaggcg ttttccaccg actcgcggca
     1681 cgacacgtcc atttccaaag ggtaaaacaa agcacccaat tcatccgcca aagcctgaag
     1741 cctgtccgca cggcgcgccg caccgataac gcggtatccc gccccgacaa acgtgcggca
     1801 catcgcttcg ccgaagcctg ccgaagcacc ggtaattaaa acagccattg tttttccttt
     1861 ctttctcgtt tcccaaacct gtttcaggta tcataacacc cttcagacgg catcgcgccg
     1921 gctgaaaacg acaacaaccg taccaccacg acaggacaca ttcaacatga aaacccaaat
     1981 cagccttgcc gcaaccgccc tcgccctatt cctttccgcc tgcggaaacg gcggcgcgcc
     2041 cgcacagccc aaaggcgaaa tttccgaaaa ccgcaccgcc gcgttcaaat ctatgatgcc
     2101 cgacttctcg cgtatgggca aaatggtcaa aggcgaagag ccttacgatg tcgaaaaatt
     2161 caaacaggcg gcggcggcgt ttgccgaaag cagcaaaaaa ccgttcacac tttttgagtc
     2221 cgatccgcaa ggcaacggcc gcgccctgcc tgccgtttgg tcggatggtg caaaatttga
     2281 agccgaaaaa acaaaattcg ccgccgccgt cgaaaaactc aacgccgccg cccaaaccgg
     2341 caaactggac gaaatcaaag ccgcctacgg cgaaaccggc gcaagctgca aatcctgcca
     2401 cgacagtttc cgcgcgcccg aataacatcc gaaacagaac aatgccgtct gaagccttca
     2461 gacggcattt tctttgtcat ttcgccacgc gcaaagcaat cggaaattat cttcaattca
     2521 aaaaatacgc catatcctga tttttgcctt acttactccg ccattttcta ttttgattta
     2581 ccatgcgatt gatttttatc gtcaaatgcc aaaatcggaa tgtcggcctg acggaacagc
     2641 gcataaacgg gcttgagcca ataatcttcc ggaagggaag ccggtttcaa tgcgtaaacg
     2701 tgcacattgg gaaaatcctt catcgtcaac gccgcaccgc tgaaaaaggt ataaatttca
     2761 tacctcgtat gcgggttttt cttaatttcg cgcaaaatat agtcttcgat gacatagggc
     2821 gaatttaacg cggttacgcc ggaaagcccg taggtctggc ggggatgcgg cgcgacatat
     2881 tgtatgttga agttttttgc cgccttttgc gaaatttcct tcatctcttt gtcgggcgaa
     2941 cccaaaagta tccgcaccgt gccgcccgtt tcgtcccccg ccttcagttc ggacgcatcg
     3001 aacagcggca ggtaagtcat cttgcggcgg ccgtcgtcca taatgttttt caaacccttg
     3061 aatatcgtgt aatgctcgtc ggaagcattg cgggttttgg cgatgctcca atctccaatc
     3121 atcatccggg caaaattccg cttaatcgcc ccgtttacat aaaactcatc gcccaaatag
     3181 ctgctctccc gtatcaggtt gtttgtgccg tcgtcaaagg tcttgatttc cgcatccggg
     3241 taagtactca aaaaggcggc aatactgact ttttccaaac tcgccaaata aatccgcctg
     3301 accttcggca gcagcatcga cttcaccttc agctccgcca tcgtcggaat gaaattaaac
     3361 gatttgttca aaccgtaggg caggtagaaa aaatacgccc gctccgcctt atcctttatc
     3421 tgattgaaat aataatcgta tttttcattc ctgttttcag acatcagcac cacataaaac
     3481 cgctcccccg gatgctgcgc cataatcctt tctgccacct tcatctgcaa tatggtatag
     3541 cagaaaatca gattgacggg tttcccctct tcattgaaga gtttgtcctt cagcagggaa
     3601 accgcgttcc tttccccctg atttacccgg tcaaacgtat aaaatatccc gaagcaaaaa
     3661 acaatcaggc acaacacggt caaacagact tttttcaacc ccatatccct aaaaactcca
     3721 ttccgacaaa ttgaacatac gccccgagtg tcccggtgcg gaaacccggt gcggaaaatg
     3781 cggaatttta gcagatattc cgacagttgc cgaatattaa aatcaatttt accgaaaatg
     3841 tgccgccgca catcaggcac aacgccgccg acgccgccgc caaacgcggg aaagccattt
     3901 cccccttcct ttcccgccgc cgcgttattt cccctttcct ttcccgccgc cgcgttcaaa
     3961 cacgccaaga tcgaacaaag cgcgaacaaa gccgccaaaa tatgccataa tagccgcgca
     4021 cattccccgc gcaatgtgtt tcaaaagaaa aaaccaacct acaagagaat aatcctatga
     4081 ctcaaaaatc caccattgtt tatacccata ccgacgaagc ccccgcgctg gcgacccaat
     4141 cgctgctgcc gattgtgcag gcgtttgccc gccacgccga tattgatgtc aaaactgccg
     4201 acatttccct gtccggccgc attttggcgg cattccccga atatctgacc gaagcgcagc
     4261 gcgtacccga cgcgcttgcc gaattgggcg aactggtgaa acaacccggt gcaaacgtaa
     4321 tcaaactgcc gaacatcagc gcatccgtac ctcaactgac cgccgcgatt aaagaattgc
     4381 agtctaaagg ctttgccgtt cccgactatc ctgccgaccc tcaaaccgat gaagaaaaag
     4441 ccgtacgcga acgctacgac cgcatcaaag gcagcgcggt aaaccctgtc ctgcgcgaag
     4501 gcaactccga ccgccgcgcg cctaaagcgg tgaaaaactt tgccaaaaaa aatccgcaca
     4561 gcatgggcgc gtgggcgaaa gactccaaaa cccacgttgc caccatgcaa agcggcgact
     4621 ttttccataa cgaacaatcc gttaccgtac ccgatgcgac ttccgtatcc atcgtgttca
     4681 ccgacaaaca aggcaataaa aaagagctgc gcgagcctgt tgccctgaaa gccggcgaaa
     4741 tcattgacgc ggccgtaatg agcaaaaaag ccctgctcgc cttccttgcc gaacaagtga
     4801 aagacgcaaa agctaaaggc gtattgttct cgctgcatat gaaagccacg atgatgaaag
     4861 tgtccgaccc gattatcttc ggacacgccg tcaaagtgtt cttcgcgcct gtatttgaaa
     4921 aattcggcgg caaactggct gccgcaggcg tcaacgtcaa caacggcttc ggcaacctga
     4981 ttgccaatct ggacaaactg gatgcggaca cccgcgccgc cgtcgaagct gaaatcgccg
     5041 ccgtttacgc cgccaacccg gatttggcga tggtcgattc cgacaaaggc atcaccaacc
     5101 tgcacgttcc cagcgatgtc atcgttgatg cttctatgcc tgcgatgatc cgcaattccg
     5161 gccgtatgtg ggacaaaaac ggcaaagcgc aagacaccaa agccgtgatt cccgacagca
     5221 gctatgccgg tgtttaccaa gcaaccatcg atttctgccg cgaacacggc gcattcgatc
     5281 cgacaacaat gggtactgtg cccaacgtcg gactgatggc gcaagcggcg gaagaatacg
     5341 gctcacacaa caaaaccttt gaaatcgaag ccgacggtca ggttcaagtg attgatgcgg
     5401 cgggcaatgt cctgatgcag cacgacgttg aggcaggcgg catctggcgc atgtgccaaa
     5461 ccaaagacgc tcccgtcaaa gactgggtac aactcgccgt caaccgcgcc cgcctgagca
     5521 acacgcccgc cgtgttctgg ctcgacgaaa accgtccgca cgacaagagt ctgctcgcca
     5581 aagtcaaagc ctaccttgcc gaattggata ccaacggcct cgacatccgc gtcctcgcgc
     5641 ccgaagaagc cgctaaattc agcttgggcc gtctgaaaaa cggcgaagac accatttccg
     5701 taaccggcaa cgtcctgcgc gattatctga ccgacttgtt cccgattttg gaactgggta
     5761 ccagcgcgaa aatgctgtcc atcgttccat tgatgaacgg cggcggtatg tttgaaaccg
     5821 gcgcgggcgg ttctgcaccg aaacacgtcc aacagttcct cgaagaaaac cacctgcgtt
     5881 gggactcatt gggcgaattc ctcgcgcttg ccgtatcgtt tgaacatctg gcgcaaaaaa
     5941 ccggcaacgc caaagcccaa gtcctcgccg acactttgga tgccgccacc gaaaaactgc
     6001 tgttgaacga caaatcgccc aaacgcaaag cgggcgaact cgacaaccgc ggcagccatt
     6061 tctacctcac cctctactgg gcgcaagaat tggcggcgca agacaaagat gccgaactga
     6121 aagccgcatt tgctccattg gcagccgctt tgactgccga cgaagccaaa atcgttgaag
     6181 aactctctgc cgtacaaggc aaagcggtcg acatcggcgg ctactacgca gccaatcctg
     6241 aaaaagccgc acaagcgatg cgtccgagcg caacctttaa tcaagtacta aacgccttat
     6301 aagcacaaag gcaaaaatgc cgtctgaaca ttcgtcgttc agacggcatt agttatccct
     6361 atctgcctga ttatgattaa acgccgcacc cattggatat atagtggatt aacaaaaacc
     6421 agtacggcgt tgccccgcct tagctcaaag agaacgattc tctaaggtgc tgaagcacca
     6481 agcgaaccgg ttccgtacta tttgtactgt ctgcggcttc gtcaccttgt cctgattttt
     6541 gttaatccac tataaatcat ataatcttta gttttttacc ctacggaaag acaataatat
     6601 gaaaactgtt ccaaccatcc tgaccggcat ccttttggca accgcccttc ccgcatccgc
     6661 acacggtatg cataagagca aacccctggc tatggacgag ctgccgccga tttgccaaca
     6721 atatttcaaa cgcaccgaaa cctgttacaa caaagccgga aacaaagcag atttcgcacg
     6781 caacaacacc aaattcctat tccaagccct acctgccgcc gatttgggac agcgtaagca
     6841 aatgtgccaa atcgctatgg attcgtttgc agaaaaaacc cgcaatctga attgcgaata
     6901 aagccgcatc agcaatagca atgccgtctg aaacaaaggt tcagacggca ttctcacatc
     6961 aaaacgtacc ggctgataaa acccgccttc cccgcatccc tgacccgaac aggattaaac
     7021 ggagtaacgg tttcggccat tatggcaagt ccccatccca aaaatgacca tataaattaa
     7081 taccataaaa cattgataaa aagcgtctgc cggttgcggt ttgttgcagg gcgttacgga
     7141 aaatcatcgt aaaccgatga tttttatata gtaattgaca caacctgaac aaaaaagaac
     7201 cgccctgaat cagggcggtt ttgctttgcg gcggaaacgg caggattcga ctaaatttta
     7261 ttttattgat ttaaatacat ttatttctta taaaaattta atttaccata aaaacagcca
     7321 tatacaaaaa tcttggagta actactgcat tacactatta gaaggatgca gggtcatgta
     7381 atatttaatt aaattaatat ataaattaaa tatttgaatt atatttataa aaaaaggtgc
     7441 aaataaggtg caaataaaat tagtttttcc caatgggtta aagaacaaat ccgaaaagag
     7501 ttcttaaaat agcccgaccg ccgccaagcc gcaaacttgg cggcttcttt atgccgtccg
     7561 aaagcccttc agacggcatt tttctttgat tttgttgggg gaatggttga ctttgaacgt
     7621 gtccacgttt ataattcaat tcatcggcgc atcgggtgcc gatacgggaa aagccccacc
     7681 gaagcggggc ttgtacaggg agtaggaaaa tgaagctcat ccttcaaatt ctcatcctgg
     7741 ttttaatctt gatgctggca ggcgatacgc ctattagcgg ataaaaccgg ctggggcggt
     7801 agccgccgcc ccagtcccta tcttacaaaa ggcagttcaa aaaatcaagg agtttgccta
     7861 tggttaatga aaaactggca gaaaaccgaa agcgttacga gcaaaaacga gtcattaaaa
     7921 aagtctcgtt taatgcggaa acagaaaaag agcttttgga atatgcccaa aatctcgatt
     7981 tttcccaatg ggttaaatca atcataaaag aaaaaatcaa aaagtaattg actttgaacg
     8041 tgtccacgtt tattattctt acaacggcac agccgttaat ggaaaaccga accaagcggg
     8101 aacttggttc ggttaagtga aaagcagttc taggaaaact cttttcgcgt cttttaaata
     8161 acctctaaaa gaaagactat tctatcatga atgctgtcca agttttaaac tttcaacaaa
     8221 actccgtccg tactgttgcc gataacaaag gcgagttgtg gtttttggca aacgatgttt
     8281 gcgagatttt aggctacacc aaccctcgcc gaacggttga cctgcactgc aaaagcaggg
     8341 gcgtaacaaa acggtacacc cccaccgcca gcggcgaaca ggagatgacc tatattaacg
     8401 agccgaatct ctaccgcttg attatcaaat cccgcaagcc tgccgcagaa gcgtttgaag
     8461 aatgggtaat ggaaaccgtc ctgcccgcca tccgcaaaac aggcggctac caaattactc
     8521 ccaaaaccac cgccgacgac cgtaccggat tgcgccgggc tgttgccgcg cttgtcggac
     8581 gcaaaggtat agactactcc tccgcgtaca gtatgataca ccaacgcttc aacgtcgaat
     8641 ccgtcgaaga cctccctgcc gggaagctgc ccgaagccgt cgcctacgtc cacgcattga
     8701 cactgcacac gggcttgaca ggcgaagtgt tggacgcacc gcccaaagcc gagccgaaac
     8761 tgcccatcga cggcaactct ttagccgaca ttgccgctat ggtttattac ggcacatgga
     8821 tgattgaatc gggcaaagac atctccgcgc cgctgaagca gctcggctgc aggcaggcgg
     8881 ttacgatgtg gacggtttgg cacgaaaccc gttcaatcct gaaaagatcc gccgcggccc
     8941 tcgaagtgtt gcggggatat gcggacaaag acgcctccga ccgcatagcc gcatgtcttg
     9001 aaggcattta cggcaaggct acggtaaggt aaacggaaaa tgccgcccga atcttcggac
     9061 ggcatttttc acgcggcata gcgtttcaac atttcccgat aatccgccgg ttcccgtccg
     9121 gcgaactcaa aggccttcaa atcggcgtta tctgatgcct tgcggctctc gttcagccaa
     9181 taggcgatct gtttcctgca aaactcttca gcactcatcg tcaaatccaa aaatgcctgt
     9241 tcaggtgcgg cgcatcctca cgcgtgatac ggcgcaaatc actgtcggcc ggcaggccga
     9301 aatacagctc gaaacgcctc cttgccgatt ccgaacgctg cgggtcgaaa gtctccgcat
     9361 ccgggatact gaaagcctcc gccaaagccc aataaaccaa attccgatga tgtatggaat
     9421 tgatttccgg ctcgtccccg tcccccgcca tatcccgcgg caggcaatac cccccggcaa
     9481 acaacctgcc gtcacggtcc ggcgcaggcg caagcgtcag cttcccgtcg ccctgcaccg
     9541 cataaaccgg cagccccgtt tgcgccccgt cgcgccaacc cggggcgcaa acatccgccg
     9601 cctccggcga aaccaacttc aggcacaccg gcttgccgtc ggacacaaaa cggatactgt
     9661 cgatttcata cgcataaccc gccgcaaacc cccctgccgc ataagcataa agcggccgcc
     9721 ctttttccac atccgttaca aacgcatcgt ccgaatgcag cagccggccg cgcaggcacg
     9781 cctcgtgaac cgcatcgttc aaccatcccg acacctcttc gtcgctccaa aaatacggct
     9841 cggccttatc attggcaagc acgcggaaac gcgcaatcaa agccttcaaa ttcattgcgc
     9901 gataccgaac tgacgaacca tccgaaccac gtccccgcgc aaattctccg ccgatttgcg
     9961 cttatccaaa ccctggccgt aattcgcctt cgcatacagt tcaagctcgt ccttgcccat
    10021 ccggttgatt tgcaggatca catcctgcac cgcgtcaaac gccgccgttt cccgttcgag
    10081 ttccgaagcg cccgcctttt ccaaagcctc cgtatcatcc gccttttcca cgcgtttgcc
    10141 cgggacggcg gcaaacacat cgccgtggcg caacaacttt gccgcagccc cggccgacac
    10201 ctcgcgcacc tggccgtctt cgaaaaccaa gcccgtatga tagatacggt cgcgccacac
    10261 ggcacgcccg ccgatatact tgataaaaac cttatccatc caaccccccg ccgccgtccg
    10321 gacggcattg tcaaatacag caacgccgca aaggcaaatc cccgggcggc gtttcattcc
    10381 gcttacaact ggccgaactt ctcgccgtaa atcaaaatat cgatatcggc ggccttctta
    10441 ttttccgccc ctttaagcgt aaccgtcagc aaagcctgct tgggcagcgt aaccagtttg
    10501 gcagactggc agcggatacg cccggcagac gcaaagtccg cgcccgaagc aaaataagcc
    10561 gcatcctgag gcactttcgc atcatccgca ccatccgcat acgcaaagcc gacatcggca
    10621 gtgataccgg aagacatcgc cgtcttcaca ataatctgcg cgtcatccaa acggaaaccc
    10681 tgttccaaga cacccagcgc caacacatcg cccgccttag gcggctcaga agcatccgcc
    10741 cccaagatcg caccgtcctg cttcgcaacg atacggtaat gctccgccgc cagattgccg
    10801 taaggcacgc cgccgaaacg gttgccgcca ttgtttttca cataaatctc agccataaaa
    10861 aacctcaacc caacaaatag gaaaagccgc cctttcaagc ggcattcgaa tcagcggata
    10921 ccgccgtgca aaggaaccac cgtatccaca accgtgacac cgtagtcggt aatttccttc
    10981 gcgcccccgc cgacatccac ggcaaagcgc gttttcgcca caccgcgtat cgtaccgacg
    11041 agcgtttcca cacggttgcc gtggtcaagc tccttctcag accaaaagaa aggcataccg
    11101 ccgtgtttgc cggtattcgc aaacgcctcc aacaccgcct ggccgcccaa aataaccgaa
    11161 cggtcgaccg caaacttgtc cgcaaacgaa gccgggattt tcaagcccga ttccgcctcg
    11221 ctgaacttat cggcgcaata cttcatctca tcgcccgcat agaaacggat ggggcgcggc
    11281 attttcacca ggataaagcc gttccacaaa cccgcatcgc ccaaaaacag cggattttga
    11341 tttgcctggg aggcgcgcgc gattgccgaa gcctgaagct ggcggaattt cggatcggcg
    11401 gcaaaacggt tgtactgcgc cggggaaagc agccacacgc gcaaaggcga atcacccgcc
    11461 gccttgtcgc cctcaaactt cacaatcggc ggcggcagcg gaatctggtc gagcacggta
    11521 cgcatactgt ccaccgtatc catcgtaaac aaatcggtag aggcaatctt caactcgccg
    11581 ccgttgtccc caacgcccgt taccgcatcg cccgaagccg taaaatggcg gtttttggac
    11641 ggggctttga ccggattgac cgcataatca ttgaatttcg ggtcgcccgc caaaggcacg
    11701 ccccattcga tattgtcatg aaaaccgcgc gcgcccgcca tatgcaccaa cagcgtctgg
    11761 tcggcataac ggtccatcag gctttgcgcc gccggacgaa tcagcgcgcg ataatcggca
    11821 gggctgcgct gattcgtcat cgtgccgccg ccgtcaacgg ggaaacgcgc ctgattcaca
    11881 cgcaaacccg cctccgacag gctcatcccc acgcccctgc cttccgccgt gttgtcgccc
    11941 ataatcggca gggcggaaac agggttgacc aaattgaaac ggatttcgtc acccatgccg
    12001 cgcgtcaaat cctgacagcg cacgaccggc atatgctggg tcgtctgttt gcgcaaagtc
    12061 gcctccgcac ccgcggtacc ggcaggcatc ttgcccgcca aacggttcag cgtactgttg
    12121 cgctgcatat gcatcgcaaa cagccccgcc gcctgcctca tcatcgcctg cgggtcgccg
    12181 taggccgtat tcgtcttttg tgccataaag agcctccctc aaaaactcaa gaatttaaaa
    12241 acttctcaac ctgctcttcc gtcatggaag ccagtttttc cgccagcgcg ttgccgctca
    12301 aataatccgg cgggccgtcc gaagccgtat ggtcgcgccc cgccggaata tccgacagcg
    12361 tattgggcgt attcttcccg gcaggcggct tttcggcggg cttttccggt tcggcggcgg
    12421 attttgccgc cttaaacata tcgaacgccc cgatgacctc ggcggcagtc ccgtccttaa
    12481 gcgcgtcatt aaacgccttg cggacaagcg ggttctgccc ggccagccac tcctccagct
    12541 cgcggctttc cacaatcgaa tccgcatcgg ggtgcgccgt atagatttcg cggtaatgcg
    12601 cctttgccgc ctcctgccgc ttcatgtccg cctcataacc cgccagcttc tcctgaatca
    12661 gcttttccac gccctttttc acatcctcgt cggaaaaatc cccgaacaac gaattatcgg
    12721 aaccgtcagc cgtttccact ccgggttttt ccgccccctt ctcaagctgg gcaatgcgct
    12781 ccctcaaggc ttgggcttcg gctttcgccg acttttccgc ctcgcgcgcc tctaccagct
    12841 tttcataagg gatagtgtgt ttgccgtcct tggcacggat gaccttgtcc gcacgccgtc
    12901 cgccgcgccg atacccgcgc cgaccaccgc gcccgtactg ccgaccacca gcgattccgc
    12961 ccacttctta ccctccaaac cgtcttggat atcggggaaa accaactggt ccaaacccaa
    13021 acgccccgac acgtccagcg gcgtcaggcg gctgaagccc ttggccaaca cctcgcccgc
    13081 cttatccccg aaagtgtccg ccaacatatt gcgcaacgcc gcttccgcat cccacgggtc
    13141 atcgtcgtca ctgcccaaca tcgaagccac cgcaagcagc gtcgacacaa acggcagccc
    13201 caagatgccc gaagccatcg catgcgagac caacagcccc gccagcgtct tacgcgcctc
    13261 cttatccccc ttaaacgcaa ggtacgcatt gcgccccagc gcatacagga tattctgcga
    13321 atactgcttg aacaggaaga ccaccttcgc cgcattgccc atcataaagc gcggacggtt
    13381 ttgcgccgca tagtcaaaat gcccgtcgta cgtcgccttt ttcgcctgtt cgaaagccgc
    13441 ctcactgtcc gcccccgccc gtttcgccaa acggtaggcc gcgacaaacg taacctggcg
    13501 gttaaacttc tccgcatgat ggaacagcca ggccgcctta tccatcacct tctgccaagc
    13561 cgaccccgcc aaccccgggt cgccgttggc cacccccgcc aaatcatgcg cctgcgacaa
    13621 atcgatcaca ccgtaatccg ccgcctttcg gaacgccgcc ttctcatccc cgttcaaact
    13681 gtcctcaatc gtattgaact tctccccgaa cctcagcccg atctgttttg aagcccgcag
    13741 caattcccgc gccgcaccgg cataacccca cttcgccgcc atcaccggat aagccaccat
    13801 tgccgtctgc gacaggttca caaccgcaga agcgggcgac atccccatca tccacaaaaa
    13861 gccgaagccc gtcaaagcct gcgccagctt ggaaggattc ggattcatca ccgcctcgtg
    13921 gcgtttcctc atctcatccg ccacacgctg caactgacgc tggtcgaaac cctcctcata
    13981 tttgcgcccg tccacaaaat cctgcatcac atccaactgt tccgccatac ggtccgcata
    14041 gcgcaacttc gccagataat tcgcaccgct gcccatattc tgcgcatatg cgcgcctcgc
    14101 atcatcgctg aagcccggca cgcccttgcg gtggatgccg tgtttcgccc aagacgtatc
    14161 gggcagggag ttcaaataaa gctgcgtcag cgtatcgttc aatcgggccc gttgcgccgg
    14221 atccaaatcc atcctgccga cagcctcgcc cagctccttc ataaatccgc tgccgaccgc
    14281 atcgcggctt cggatataat cccgggactt catcacgggc gaaaccttaa accccggccc
    14341 gaaatcagcc ttcagcgcat cgcgcagctt ctccgcctcg ctcaaagttt ccgcgcggga
    14401 cacattcgca ctattcccgt ccgcatcctt gaccaccacc agataatcgc cgaaacgcgc
    14461 caaagggaaa tacacaccgc ccagctcgtt gtcaaaacgc tccttcaggc ggcgcaccgt
    14521 ttccgcatcc tgccccgcac gcgccaaccg ttcggcaagc gcatcgcgca cctgcccgaa
    14581 atgcgcccta taatcatccc gcgccttgcg gtacaccctc tgcgccgcat caggcagccc
    14641 ggcaaaacgg cggttcagca aagcaagccc ctccaaagcc ttttgcgccc caacctcatc
    14701 cttttccaaa cgcttcacat ccgcgcgcgc ctgctccaac atccgtaccg catccgtttt
    14761 agcccggctt tccgccgccg catccgcccc ggcgcgcctc aacgcccgct tcgcctccga
    14821 atccgcataa aacagacggc gcaggcgcat atccgcctca tccgccaaaa tttcccgacc
    14881 gtgtttttcc tgcgccttct ccagcgcata agccgcctta tccgccgccc gttgcgcctt
    14941 attgtaagca gcatccgcac gcgcggtacg cgcaccggcg gaagcaacgg ccgcccgcgc
    15001 cttcgcgatt ttaccgtccg caatatccaa agccgtccgg atgccgtcca aacgcccccg
    15061 ggcatccttt cgcatcagcg ggtcggcatc caccttcgcc agcgtcgcat cgtgcatcag
    15121 atccgcaagc gctttcgcat cctcctcctt caacctgccc cattccctga caaccttgtc
    15181 cgcctccgta accgccttgt tcgcatccgc gccgaagagt tccgacaaac gcccgtattc
    15241 cttcaggccg tccaaaacgc cgccgtaaat gtcctcgatc tgccgcctgc ccaaaaactg
    15301 gagctgaatc cccttggaag catcccagcg gtctttcagc gcgtttctca aatcggattt
    15361 tttacccgtt atacggctga ttgcggcccc gatgttcgcc gcacgggaaa aacgcaccgc
    15421 ctccgtgtca aaatcggcaa tgccgccctt gcccgcatct ttgtcttgca aaatctgcaa
    15481 caggcgttta tcatcaatgc ccgataaagg cgatttgctt tcagaggctc ccgtagttga
    15541 tacgcggtca agtaatgaaa gcaagttgcc tttttctatt ttcgtcagct tatggtcgta
    15601 atagcggtta ccgttcctgc taaccgcgac ggcagccctt accgtgtaat ccgcaccgcc
    15661 gacattcagt cccgaaacat aatattcata accctgaata tcgccgttct tagccaaatc
    15721 ctcgttcggc agcgtatcga tatacaccgc attctcaata atctgcggaa ttgccgcgat
    15781 actctgcaaa tgttccgcgt ccttatagtc gtgacgcaat atttcagtga tgcccgaacg
    15841 tcccaaactg atttcacgcc ccgtgtcctt attcacataa ggcccgcgca aagatttgcc
    15901 gtattccagc gcattgcgct tatattcgcg caaattgccg ccttccggaa cctccctgcc
    15961 cgagatacgg atggtttccg cacggcgcag cttctcaagg ctttccgact tggaacgttc
    16021 cgccgcaccg tcttcagccc gcgcgaacat cactgcacga tgattgccgt cccaaggcgc
    16081 atcccgcgcc ccttccacat tcccgtgcaa atccgccagc atcgcaaaca catccgcgtc
    16141 cgacatcgca ccgccggcct tgcccgccag gcgttgcagc acacggcgca acagggcgcc
    16201 gatacgcgcc aacacgcccg caatgccgcg tttcaaaccg ttgccgacct tcacgccgta
    16261 acggttttca atgcccgccc aaccgccggt acgctgcgcc gcataaagct ccgcgaccgc
    16321 ctcttccacc gcggcggagc gcaccgaggc cgccgcatcg cccgtgcctt cgcgcccttc
    16381 ctgcaccgcg tccgcaatgc gccgtatcag gccgttgccg tccgcacgtt ccaattcttc
    16441 acggtacttg gcgaaaccat ccgccgcaaa gcccctgtgc cccagctcgt gccacgccgc
    16501 ccataccgcg cgttcgggcg taaggttttc cgccaccagc gtgattttgc ccgtcctgcc
    16561 gtcaaaccag ccctccacac gctgcgacaa cagcatctgc gccttgtccg gcgccgtttc
    16621 ggacacggcg gcgacatcca cccgttccgc cagtccgcct atgctttcgg cgaggcgttt
    16681 tttgatattt tcggcgtgct cgcgcaatgc cttaaaacct tgcggcgcct gacgctcttt
    16741 caccgtataa acaggccctc cctgcaacac acgcgccttg cgccgtgcct cttccggatt
    16801 gtccgaagcc gttttcgcaa acgtttcagg cttggcagcc ttatccgaag acggcttttc
    16861 ggttgccgcg ccttgtgcgg cggccgactt gccctttccg ccatcgaagt gggattttcc
    16921 cgattcgata tttcgcacct tatccgaaga cggcttttcg gttgccgcat tttttgcagc
    16981 agccgccttg cccgcaacag gcgtatcttt tgcccgggca ggctgcgccc tgcccgccgg
    17041 tttgccgtcg gcggcggcat cgcgcggccg cgccatcacc ctggccggcg cccttgcttc
    17101 ctgcgtcgga cgataatccc ggcccggctc ggacggcacg gcttccgaca aaccgcccgc
    17161 ccgcaaggtt tccgcctccc gccccttcaa ataacggcgg ccgtcggaga caaggccgtc
    17221 agagttggaa ggaccggcca tatcggcaaa ctcctccggc atcccgcctt tgcccggcct
    17281 gcccggcacg cccgcaagcg catcaatatg cctgcggtcc gtatgctcga aacggtattc
    17341 gccgcccccc aaaggcacac ccgccttttc cggcctgcgc ctgtccgccg ccgcatccgc
    17401 catccgtttc ttaggcgata ccctgcccgc cgaagccgca acctgcggat tcaataccgg
    17461 caacaccgta tcggggcgtt tgcccgcaac aggcgcacgt ttcccgccgt ccgtcccggc
    17521 aaggttttca gacgcggaca ggccgggcgc ggcaccttcc gcgcgcgccc gaacctgtaa
    17581 gttttgccga cgggccggcg cattgccgga cgcaacggcg gcggaccctg gcaaaccctg
    17641 caaggcagcc gcatttgcat ccgcccccgc atcaggggtc aaacccctgc cgccttgggt
    17701 caaaccctgc cgcacatcct gccgcacatc ctgcgccaaa cccttatctt ccgtgggtaa
    17761 agcccgggtc aaacccctgc cgccgccgac aacggcctca gacgcgccgt catccggcaa
    17821 cgccgtccga accccctgca aacgctcccc ctcgcggctc aacagcggca cgctccaacc
    17881 gttgttgcgg cggtaaccgt aaaccgcacg gttaagcctg tccaacacat ccgccgattc
    17941 cggatgttcc gcacggtaag cgcgtacagc cccggccgat tccgccaaac cctcaggata
    18001 atgtccgcgc atatccgcca tacccgcctc ccggcgcata atcccgtccg ccgtatagtc
    18061 cgccaccgtc tgcgcatgac ggcgcacccc ttccggcagc gcgtcaaacc gcctgcgcga
    18121 ttccttatcc aacgcatcgg cagggacgga aaaccctttt ttaggaacag gcacggcatc
    18181 cgccatacgc acgggcggcg gcaggtaacc ctgtttggct tcggaagccg cctgataatc
    18241 cgcctcaatc cgcgcgtgat acgcctgctc gatttttcgg cgcacaggcg cggcgatttg
    18301 cccgaaatcc gcaacaccct taatatcaag gcccgcctcc ttcgccaaag ccttgcgccc
    18361 gcgcgtatcc aagccgtcga aatattggcc cgcaggcagc ggcgagaccc gcgcaatccc
    18421 gcccgaagca gcggcggcag aaccgcccgg cgcaaccgaa gccgcaccgc gcggcgcacc
    18481 gccggggcgc acattgccgt aagcggacgg aacaccgccc ctgtccgcat ccgccgaccg
    18541 tccggacggc gcatcatccg caaccgaccg ggcttccata tcgtcagaca ccgcagacgg
    18601 cacagccgcg cccgaatccg ccgccaaagc agcggccgca gaaaccgcac cgtcatccgg
    18661 atcaatcccc accgcctccg acggcttggc ccggtattta gccgccatgt ccgcatactg
    18721 acgcgccaaa ccgtccggca tcacgcccgt atccaaaaat tccgccccgt cctccgacgg
    18781 gatacccgaa acatccggct gtttttccgt cccgcgcgcc aattcctccg cgtgcggcgt
    18841 attgccgtcg gcagggccgc tgaaaaaccg ccccgcctcc tcctgcttcg tacggtccgc
    18901 aaactgccgc aaagccggat gcgactgatc gtgatgcgcc gtatcaaaac ccgcgccgcc
    18961 ctgttccgcg ccgccgtccg cagccgcctg tgccgccgtc cgcagccgcc tgtgccgccg
    19021 cacgcaacgc ctgcgcccct tccggggccg gcctgcccgc cttcctgttg cccccgatac
    19081 ccccctgcgc acccgccttc cgtccggccg gcggcctgag cgggtcagtt accgccgcat
    19141 aagccttcgt tccggcaaac cccaccgcag aatgccccaa agaagagaaa gcctccaaag
    19201 ccgccccctt ctcgtcccat tccccgtttg ccagccccgt acccaaatac tcgcccgcaa
    19261 actcaccggc cgattccgtc gcataagccg ccgcgtgccg tgccgcaccg cccaaaccgc
    19321 ccttggcaga ctccttcgcc agcgccgcaa attcaggcgt tcccttagcc gccgcaaccg
    19381 ccgccttatc cgccgccgca acacccattt tctccaatgc ggctttcccg gcggccttct
    19441 tgcccatatt caaaatactg ccgccaagtt tcatcgccgc catatccgcc gcgccgacca
    19501 ccgcgccctt gaccgccgca tttttcaacg cacccggcgc accgcggccg ataaacgcca
    19561 tcaccgcatc cttgtccgca gggtcgacgc ccgccgcctc tgccgccctg tccagctgcc
    19621 cgccgtattc catcagcgta ttgcccaagg tcgcgcccgc ataggcgccg acagccgcgt
    19681 taccgcccgc caaagcgccc aatatgccgc cggcggttgt cgtagcaata gggacaagcg
    19741 cattgggcgc ctgctccagc accgcacccg ccatctcgcc cacatcttcc aaggcgccgt
    19801 ccaaaccctt ctcgcgccag tccttcttaa gctcccccca cacattcgac aggccgccgc
    19861 cgacaccccc gcccttttcc caagcctcgc ccagctccct gcgcgcttgg gatttgcgcc
    19921 cctcctgaat cttccgataa tccatatcct cggccttgag ccggcccagt tcctctttgt
    19981 cgcccgtaag ggcattccac cccatacgcg ccgaacgcat agaattttta aacccgcgcg
    20041 tcaacgccgt atcgtcggcg gccgcccgcg catccttttg cgtaaagccg cggtactcct
    20101 tcaaaccccc gacaagccgc ccgtgttcca acggatcgta tctgaccaaa tcactcattg
    20161 ctcaaccctc ttctcaccct tctcattctc ataaatgatt ttaccgccgg ccgttttctg
    20221 accgttcggc ttaaagccca acgccgcata acgcttctcg cgctctcccg caaactccac
    20281 atccaaaggc ttgcccgtcc gcaaatcgac aatcccctcg cgcttcgccg tcaagcccgt
    20341 atccgggtcg gcaacctcat aactgattgt cttgaacaca ttcgggtcaa agcccttctc
    20401 cccttgccgc tcaaacttcc cgttaagcgc atcaatctgc cgcgcaatct ccccgcgctg
    20461 cccgtccgac ttcgcaaaac cataagcccg cgtcaaatcg gcgatgcgct tcctatcctc
    20521 aagctccagc gcatccgcct cacgcttcat ctcaaacccc tgccgcttca tccccagctc
    20581 ggcctgctgc acatcaaacg ccctgtccgc attcctctgc gccatctcgc ggttcaacgc
    20641 accctcgttc gccttaaccg ccgcatcaaa ccgccccttc tgcaacaccg cctcacggtt
    20701 cgccgcattc tccgccgcag ccgccgccag cctttccctg cccaattccc tgaccgccgc
    20761 atttttatta tgttccgcca caatcccgcg cgccgcatta agctgcgccg ccgtcaactg
    20821 accgttcaaa gcccccgcat aaggcgtagt cgccctcctg taaagctccc gctcctcagg
    20881 cgacagcgca tccggcccgg cagacaccaa aggacgctgc gcgtcaaaat cacgcaccgg
    20941 tttgccgtcc cgatacacat tcccgtcaaa tcccaaaggc ggcggcgtcc gtccgcccgc
    21001 atccgtcggg cgcgtatcgc gccgcgcgaa atccgccccg gcccgcgcat cgggggaaag
    21061 cgccttatcg ccgcccgaaa accccaaacc ggcatccgca ccccgttcga aagcacccgc
    21121 ccggcgcaac cgccgcgccc catcctcaat attgttacgg ctcaaatcca tcacattccg
    21181 ataacgcgcc ggcccggcct cataattcgc aacggtacgc cgcaccgcat cggcacggtg
    21241 cgcctgtgcc gccgcccgct cccgctcctc cgcccccttc cgggccttct cttttttctc
    21301 cttttccttc atctcccaaa aatccccggc agacggctca aacaaaccca tcgcaaaccc
    21361 tccgaacaaa taaagcccaa aataagcaaa aacccaaacc caatcaaacg cctaaagtta
    21421 agaacaaaca aaaatcgcaa ccacaaacca attaataaac aacaagttac acaaaaaaac
    21481 acaccgacgg gccatttccg ggggcaaata aaaaccgcct tcgtgcaagg cggttgcaaa
    21541 aaagcctttc atttaacgaa aggctttttg ccgcccgcgt accgcgttca gaggttgtcc
    21601 gacaccgtcg gcgtttccat gcctgtttgg gcgtgattcg cactctttgg cggcacggcc
    21661 gtcatcgggc cggtatccgc gccgtaggct tcagggatgc cttcgggcgg tatttgcgtt
    21721 tcaggcggca tggcggggac ggggaagccg ggatcgtcgc cccgcgccgg ccggatatat
    21781 cccgcgccct gcatgaccgc gtcggcaacg ggggcgattt gcggcatggc ggctatctgc
    21841 ccgcccgcct gcatggccgc gtaagccgcc tgcacgccga tttgtaccgc ccgcgcttcg
    21901 atttccttga tttcgctcgc ggtacgttgt tctttaagtg ccaattcccg ccgtttgatg
    21961 tcgttgccgg attttgccaa tgcgtcctgc accgcctgcg cgatacgcgc ctcgatttgt
    22021 tcgggcgttt cctgcactcg gacttctttg attttttcaa tcactttgtc tttaaacggg
    22081 atgtccatca gggacaccat aaacggcagc accgccgcct gatattcggg cggcagggat
    22141 ttgaccgcct cgctcatcgc acccagctgc tggctgcggt aggagttggt gctgggcacg
    22201 tcttccaaag ccactttcag ccgtatgttt tgcaggtcgt tggacaaata agccttgccg
    22261 gttacggggt cggtttcagg cctgttgatg acgaccgtcc gcccttgcgt gacggcgtcc
    22321 ccttctatga cgacggtttg ctcgtccgag cccaaatcct cgataatcat cgcaagcagc
    22381 aactcgccga ccaatgagcg gctctggcgg aagttgtcca taaccagccc cagcgactgg
    22441 ttggactgct cgacttggat gctttcctgc ctgccgctgg tggcgttgcc ccggttgccc
    22501 ataaatgacg gggtaatccc gctgatttgc cgtattgtcg cgcggctgtc ttgaagcatc
    22561 tgccaatgct gtgccgacaa ttcgaaatcg cggctgacgt cgaaacgcgc gcccggctgg
    22621 gcggcctcta ttttgttcag cacgatgtcc gcgtccaccc gtgcgatatt gcggcggaac
    22681 tgttcgtccg acatatcgac tatgcctttg gtgcgtaccg tgcgtattgc cgacaaaccc
    22741 catcgtaatt tgctgttggt gctgttgagg ttgtcctgcg cgtatttcat attgcggaca
    22801 tatccgtagg ggatgccggt gttgtcctcg cggaatccga aaaacgggac gtaagggaac
    22861 ttttgatgcg gatacggggt cggctcgtcg cggacgacca aatcccccac gacaaaggcg
    22921 cggcgcatac gcgggacgga agcggcaaac aataccgcgc cgtttgccgc catctcccga
    22981 tggtttgggt ttgcgccgtc aaactccacc gtgcgccctg tttttttgtc gcgcaggcag
    23041 tctgccgtta cccaccgcct gtaccatact tccgccaccg ccagttcgcg cgtggtttca
    23101 ttgaaccaaa actcttcgct gacggtgttg cgccccgaaa taccccaagc gtccgccagt
    23161 ccggtattgc cgccgccgtc aagcatttcc ccgctgatgc gccagtccga accgccgcgc
    23221 cccatcgctt tgaagtgtcc ggcatattca gggaagaatt gcgccaggcg ttccggcagc
    23281 agccagcggc ggcgtatcag ccagcgggca tcggacaggt cgtatttgta agatttcata
    23341 tcccaatgga tggcgttgcg gtggatgacg ccgcactcat aaggaaactc gaaagggttg
    23401 gggttgcgtg taacctccac ccagccgatg ccgcaggcta tctgccccct gaacgcgtcc
    23461 gaacaagcct tgtcggcacg gctttgccgt tccgcgcggt tgagtttgaa gttcaatgcg
    23521 tccgccacgt cccgcccgcc ggtttcgccg tccgccgtca cgcgccagtc cgtccgtatc
    23581 gtcgcctcat atccctgtac cgacagcagg gtcggggtaa tcaggttttc aacggcgggg
    23641 ggcaggccca actcgcgctg tttctgtaaa agctcgttgt caagctgcct gccgtcggcg
    23701 tagtccattt ccttgtcggc aacggcacgc catgcaggtt ggttcatcat ttcaccgaca
    23761 aacagccggt attccccgat agtcagcggt tcgccgtttt tatcgggcaa tacgcctgtt
    23821 tccggtacgt ccgtccccat caataaagcc tccaatccga aacgggcgtt tccgtgtatc
    23881 ccgtattgct gcctgtatcc attaaatcaa ccgcctgcgc caggtagcgg aacatatccg
    23941 cgccgtgcga atattcgtcg tgcagcggcc ccattgccac gcctgttttc gcatgtattt
    24001 ggcggccgta ccgtttcagg cattccaaaa gccgcgctgt tttgtcttta tcgaagtaca
    24061 ctttgggaaa cagcatccgc gccgccctga tgccttcttc gataccggtc gcgttttgca
    24121 cgaaaaccga cttgcgcccc agtccggtca aaatctccat cgtacttttg cctgtttgga
    24181 agttgcgcgt cctgccgtcg tgcggcagga agtccgtccc ccaccggtag ggcagctttt
    24241 ccaattcggc aacataccag tccaacgtcc ggtgcgtgtc ttcgatgtag ccgatgatgc
    24301 gcacgctcgt caaatcgcgc tgcaccagcc cgatggtcat ggcatcgttc cagcccaaat
    24361 cccaaaccgt atgcacgggc aaagaagaat cataagggac gagtgtaacg cgtccggaat
    24421 gaaaagcgtc ctgtatttca tgccggtaaa ccgccccctc cgataccatg cgcggcctgc
    24481 cttcccaaat attcccgtag tcctctttat tcatcgaacg ctgtgctttg agccgctccc
    24541 ggttcaatgc ttcgggaaac cacggattgt cgcgccagtt gatttcgcac aaccaagtgt
    24601 cttcggacgg catagcgata aaacgccggt aggtttcgtc cgtctccata tcgggattga
    24661 gggtaatcca aatttccgaa ccttctttgc gtatggtcgg cgtgagcacg tcccagcttt
    24721 ttttactgac gccgtgccct tcctcaaccc atacgatgtc gataccttca aacgatttga
    24781 tgctgtccac ggtatgcgac tgcaggcccg aaaacacgaa cagcgtgccg tttgcgccgc
    24841 gtatctcgaa gtcggttatt tcgtagaaat gccccaaacc caactgcgcc actttgtctt
    24901 tcaacaggcg gtgtacggta tcgcgcatcg atttttgaat ttcgcgcacg cataaaatac
    24961 gcaaagggcg ttgcgccccc agcgccagca gtgcggatgc cgcgccgtgc gatttgccgc
    25021 cgccgcgccc gccgtacata accttgtacc ggcaaggctt gaacagcccg tccagttttg
    25081 cgggcaattt caaatcaacc gtctttcccg tcatcttcag gccctacaaa ttcgatacgg
    25141 atgcccgccg tcttggcgga ttccgaaatc ttcgattccg cggtatccat accgtatgat
    25201 ttacgctcga tttccaccaa aaccttaaac cccgccgcca ccgtttggaa catacgcgcc
    25261 tttgtgaatg tgtcctccgg gcttccgtac aattccgcga caatctgctc cgcgcttttg
    25321 gacaggtttt ccgccaaatt ccgatgcctg cgctgtacgg cggcaacccg tttcgcttcc
    25381 gcgtccgtta cttgtttaag gttgaaatcc gcataatccg gcgcattgcc gtttttgtcc
    25441 gcggacagca ccatcgcccg cgcctttgcc tgtacctcgt cattcaaatc gcgcgtccac
    25501 acgcgttctt tggcaaccct gccgacgtgt tgcgccgaaa cattgtattt gcggccgatt
    25561 tcggcaatgc tcaaaacccc gcgccggtaa tccagctcga ctttgcgcca atcttcatct
    25621 gacaatctcg gcattaaagg tccaacctcc cgtttttggc ggaacattcc agataattcc
    25681 gcaatactga aaactcgcgc cccatctgaa tactggaaaa ctgctgcccg aaccccgaca
    25741 tcaaagcccc caacgagcgc atttcctgcg ggttgaggtg caataccgta tcgcctattt
    25801 ccaatacgcc catccccgaa tccaataccg atacgctgac ggggcgcgtc tcgccaaacc
    25861 ctatggcggg cttgaaaatc ccccttgtca cgcggtagat tttgccgtgc tcgaccagca
    25921 gcttggtaaa tttagtgatt tccgacattt tcaggcctgt cgtttccgcc aaagcctcgc
    25981 ggctgaccac ctgctccgcg ttgtgcaaat ccacaacccc ctggtacacc ttgtcaatat
    26041 ccgacaatcc cgacatcaaa tcaaactcct aaacatccca aacgattcca aactccgccg
    26101 ccgcccgcga ttgcaggcgg ttacactaca aagccaaaat ctttagatgc tgcctctctc
    26161 gctttcactg catcttcaat atgttcaaat aaacctaata ttttattttt tctattaacc
    26221 ataatcctag cttgccattt cttagacggc ttatggaaat aaaccccagt atgcccgcta
    26281 gtattatttt taaacttccc tctatttttc atattttcag catgtgtaac atctctcaaa
    26341 ttagatattc tattatctgt cttatcccta ttgatatggt ctattgaaga agcgtgtttc
    26401 ccataaacaa aaagccaaat taatctatgt gcattatatt gtttcttaaa tatttttaac
    26461 ttcaaatacc ccaacctgcc tttgtataag aaaacctcct tgccggaaaa tccggtattc
    26521 caagatttgt aatgcaaacc gctattgaaa tactttcttg gacgctccgc ccaatataac
    26581 tttcccgtat tgtcatcata tcttaataat tcttttaaaa cctcttgtgt taatcccata
    26641 aaacaccatg ctccatagat gcccaagata taatcttttc ttgatattca gacatttccc
    26701 gaactgataa ttttgtcgtt gatataccca ttaattgccc gttaggcatt tcaatacatc
    26761 caataaattt tctttttaaa aattcatgcc atacatcttg actaaacctt ctcccatcga
    26821 taaaaacttt ttctgaaatt tcacgataca aaaaccatag ccgcctgttc tgctcgacgc
    26881 tccgttttga cttgcgcggg cggattgtga tttcaaggtt gccgttttca aaccacccgt
    26941 ccgggctgtc ccaaatcgac cgcataacct gctttttgtt ttggggcgtc agtgtaaatt
    27001 ccgccccgtt catttcagac ggcctttcac gccgacaacc cccaatgctt caaggcggcg
    27061 tatcgtgcgc aattgcgaac ggcgcatata aaactctttg tcttcgcggc ttaggcctgc
    27121 gcccctgccg tcgatcgcgc cgcggcggca accgcacccg aaacccgcgc ccaaaccgcc
    27181 gcttttcaat cccgccccgt gcgtctcgcc cgggaaacgg cacaacacga ccgtttcagg
    27241 gttgtcattg cacacgcccg cgatgttcgg cgtgcattgc ccgcctttgg cggctttgcg
    27301 tatcgcgcta gacatcccaa ccctcctgtc cgatgaaatc ccaaccctcg tctgccgcat
    27361 ccgtctgttc catctccaac tcccccacct ccaccgccaa accgccgcct gcgaccggct
    27421 cgccgccata
//
LOCUS       DAXDXW010000020        27416 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXDXW010000020 DAXDXW010000000
VERSION     DAXDXW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27416
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.020"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(50..3094)
                     /locus_tag="ACFB64_000752"
     CDS             complement(50..3094)
                     /locus_tag="ACFB64_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC1209152.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPEFSLMLFHAVKTAVYWLFVGAVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMQSESKTSPVRPVFKEITLEEATRALSSAALRETKKRYIDAFEKNGTAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQEKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIHIEEPAAPDA
                     WVVEPPEVPEVAVPEIDILPPPPVSEIYNRTYEPPAGFEQAQRSRIAETDHLAADVLN
                     GGWQEETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFEDVPSERPSCRVSDT
                     EADEGAFQSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGITHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKTAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3259..3939
                     /locus_tag="ACFB64_000753"
     CDS             3259..3939
                     /locus_tag="ACFB64_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209153.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASVGGGQLAQMNYHLLAVNLVTLMVDTSAALWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     IGILDAANAFPSEILWTAALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSERGIWL"
     gene            3968..4327
                     /locus_tag="ACFB64_000754"
     CDS             3968..4327
                     /locus_tag="ACFB64_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HFC1209154.1"
                     /translation="MLSNILPLSIGAIFGTTARWLLNLAVPASLSPATGNLFANWTGA
                     LLIGIFAETVSHPQWKLLLITGFFGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGTAVK"
     gene            4405..4511
                     /locus_tag="ACFB64_000755"
                     /pseudo
     CDS             4405..4511
                     /locus_tag="ACFB64_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4932..5468)
                     /locus_tag="ACFB64_000756"
     CDS             complement(4932..5468)
                     /locus_tag="ACFB64_000756"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC1209155.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLSNDEDEITETVLMSKDEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(5502..5858)
                     /gene="folB"
                     /locus_tag="ACFB64_000757"
     CDS             complement(5502..5858)
                     /gene="folB"
                     /locus_tag="ACFB64_000757"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC1209156.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVHVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            5914..6516
                     /gene="plsY"
                     /locus_tag="ACFB64_000758"
     CDS             5914..6516
                     /gene="plsY"
                     /locus_tag="ACFB64_000758"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC1209157.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALVATTAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGEKR"
     gene            6608..7453
                     /locus_tag="ACFB64_000759"
     CDS             6608..7453
                     /locus_tag="ACFB64_000759"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC1209158.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGLYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLIRIGDKLRLFELHPADFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     VILIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLYAELHVHAPKTDGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DNKIR"
     gene            complement(7562..7894)
                     /locus_tag="ACFB64_000760"
     CDS             complement(7562..7894)
                     /locus_tag="ACFB64_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGK_0946 family protein"
                     /protein_id="HFC1209159.1"
                     /translation="MKNLKNISVVAVCAVLLAACASENSVANYAIGDDSAVIKAGRNR
                     AEARISRAELAQHRRQRKNVSEELALEREKRANKHDAIRQGMGTAAGGLMLLNGVVGT
                     VGVMKSVF"
     gene            complement(7944..8192)
                     /locus_tag="ACFB64_000761"
     CDS             complement(7944..8192)
                     /locus_tag="ACFB64_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209160.1"
                     /translation="MKMKKLILLSVAAMLVTACTYADRRFVTQESAAEIQAKSRAIQI
                     SERAERAEYRKERREEMMDAARAIKKANENSPNIYFIR"
     gene            complement(8249..8410)
                     /locus_tag="ACFB64_000762"
     CDS             complement(8249..8410)
                     /locus_tag="ACFB64_000762"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209161.1"
                     /translation="MPSERRQIAAGRSFGAFRRISRGIVVEKNGAGQVGTRQADREVQ
                     DAKFIFKKY"
     gene            8435..9409
                     /locus_tag="ACFB64_000763"
     CDS             8435..9409
                     /locus_tag="ACFB64_000763"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC1209162.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDTFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILAKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYVFTLNAENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYVDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYLQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNAYQAMLDIQPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(9568..9786)
                     /locus_tag="ACFB64_000764"
     CDS             complement(9568..9786)
                     /locus_tag="ACFB64_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC1209163.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLANNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            9980..10243
                     /locus_tag="ACFB64_000765"
     CDS             9980..10243
                     /locus_tag="ACFB64_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC1209164.1"
                     /translation="MKGTPQFPQCGFSARAVEVPTQIGRPFAFVNILENPEIRSTLPL
                     IANWPTFPRLWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
     gene            10411..12179
                     /gene="ggt"
                     /locus_tag="ACFB64_000766"
                     /pseudo
     CDS             10411..12179
                     /gene="ggt"
                     /locus_tag="ACFB64_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            12243..12407
                     /locus_tag="ACFB64_000767"
     CDS             12243..12407
                     /locus_tag="ACFB64_000767"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209165.1"
                     /translation="MSNQTKIQAIKQASEQILAICETPNTALQAIHLILRHGGAGRIV
                     LASGVSAGYG"
     gene            12352..12594
                     /locus_tag="ACFB64_000768"
     CDS             12352..12594
                     /locus_tag="ACFB64_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209166.1"
                     /translation="MAGQEELSWQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDVLP
                     LISLVLNKGDETLKTGMLNKLPDNAKENLRIMGYLP"
     gene            12661..13911
                     /gene="glyA"
                     /locus_tag="ACFB64_000769"
     CDS             12661..13911
                     /gene="glyA"
                     /locus_tag="ACFB64_000769"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC1209167.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDRRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPAKRYYGGCEYVDIVEQLAIDRVKELFGAAYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAVMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFDETDARVLSNLVADVLANPE
                     DEANLAKVRGQVTALCDKYPVYGN"
     gene            complement(13984..14373)
                     /locus_tag="ACFB64_000770"
     CDS             complement(13984..14373)
                     /locus_tag="ACFB64_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1209168.1"
                     /translation="MEVHDKIRTLREVNQWTQEEMAEKLEMSVNGYSKIERGKSGINL
                     DKLRQIAQIFNIDVVELLAEQNRSFFFSIGDNTNNHHNIIGSDEMLVFENEKLRSLLD
                     AKDELIRQKDSEIAVLKKLVILLEEKK"
     gene            15313..16104
                     /locus_tag="ACFB64_000771"
     CDS             15313..16104
                     /locus_tag="ACFB64_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFC1209169.1"
                     /translation="MKKALLALTIAAISGTAMAQLPDFLGKGEYTVRTDISKQTLKNA
                     DLKEKHKVQKNIGFRADMPFDDIHHGMRFEVSHSRDKKDMYVVTESTTKPFGKDVKEK
                     RTDVYAGYTYTQPLSEATKLRAGLGLGYEKYKDAVANEKGTVSTEREAFYTKAHADLT
                     SDLGGGWYLNPWAEVKVDLDAKLKHNATVAGVSADINAKTRGWGVGVGANIGKQITDT
                     VGIEAGPFYKHRHFKASGSFVLDGGNIRVDPTKINEYGVRVGVKF"
     gene            16255..16899
                     /locus_tag="ACFB64_000772"
     CDS             16255..16899
                     /locus_tag="ACFB64_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC1209170.1"
                     /translation="MLASVIDFILHIDQHLLSLSAQYGVWIYAILFLIVFCETGLIVT
                     PFLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRCEYLGKTRRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGIIEIARAKLAAKSER"
     gene            16918..18828
                     /locus_tag="ACFB64_000773"
     CDS             16918..18828
                     /locus_tag="ACFB64_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC1209171.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILTGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEICDLLRRYH
                     HVSHELENGSSELLLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGKKSGKIIAELEHASFAYDDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGQGRLKEYIGGYQDYIDAKSRENKIQTASAPKASDVEPAKEKPKANRTVKLSYKE
                     QRELDALPDEIAALETEQAEINAQLSDPGIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            18850..19216
                     /locus_tag="ACFB64_000774"
                     /pseudo
     CDS             18850..19216
                     /locus_tag="ACFB64_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19328..20803
                     /gene="trpE"
                     /locus_tag="ACFB64_000775"
     CDS             19328..20803
                     /gene="trpE"
                     /locus_tag="ACFB64_000775"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC1209172.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKAGGKHVDVYQNGEIVEQYDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETVYNFEHFAHRLKNTAKANPLGTPDILLMLSQEL
                     AVIDNLSGKIHLIVYADPSQPDSYERARERLEDIRTQLRQSCAIPLSLGSKQTQAVSE
                     FGEEPFKACVDKIKDYIFAGDCMQVVPSQRMSMEFTDNPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGCLKEGVTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEIEPEKRGIYGGAVGVWGFNNDMDLAIAIRTAVIKNNTL
                     FIQSGAGVVADSDPTSEWQETQNKARAVVRAAQMVQEGLDK"
     gene            20887..21825
                     /locus_tag="ACFB64_000776"
     CDS             20887..21825
                     /locus_tag="ACFB64_000776"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1209173.1"
                     /translation="MQFTSLEICAGAGGQALGLERAGFSHVALIEIEPSACQTLRLNR
                     PDWNVIEGDVRLFQGEGYDGIDLLAGGVPCPPFSKAGKQLGKDDERDLFPEAIRLAKE
                     TDPKAIMLENVRGLLDPKFENYRNHITEQFAKLGYLGQWKLLYAADYGVSQLRPRVLF
                     VALKNEYTNFFKWPEPNSEQPKTVGELLFDLMSENNWQGAHNWRLKAAQIAPTLVGGS
                     KKHGGADLGPTRSKRAWAELGVDGSGLWDSAPPEDFSGMPRLTVRMTARIQGFPDNWQ
                     FFGKKTPMYRQIGNAFPPPVAEAVGRQIIKALKKEN"
     gene            21826..22686
                     /locus_tag="ACFB64_000777"
     CDS             21826..22686
                     /locus_tag="ACFB64_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoMIV family type II restriction endonuclease"
                     /protein_id="HFC1209174.1"
                     /translation="MNPLFTQERRIFHKKLLDGNILATNNRGVVSNADGSNTRSFNIA
                     KGIADLLHSETVSERLPGQTSGNAFEAICSEFVQSAFEKLQHIRPGDWNVKQVGSRNR
                     LEIARYQQYAHLTALAKAAEENPELAAALGSDYTITPDIIVTRNLIADAEINRNEFLV
                     DENIATYASLRAGNGNMPLLHASISCKWTIRSDRAQNARSEGLNLVRNRKGRLPHIVV
                     VTAEPTPSRISSIALGTGEIDCVYHFALYELEQILQSLNYEDALDLFYIMVNGKRLKD
                     ISDLPLDLAV"
     gene            22703..23839
                     /locus_tag="ACFB64_000778"
     CDS             22703..23839
                     /locus_tag="ACFB64_000778"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC1209175.1"
                     /translation="MNTPPILPPAMLGILGGGQLGRMFAVAAKTMGYKVTVLDPDPNA
                     PAAEFADRHLCAPFDDRAALDELAKCAAVTTEFENVNADAMRSLAKHTNVSPSGDCVS
                     IAQNRIQEKARIRKAGLQTAPYQAVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTLDELKAAFAEHGGVDCVLEKMVDLRGEISVIVCRLNDENVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQTAQRLADELDYVGVLAVEMFVVGDTHELLVNETAPRTHNS
                     GHHTIDACAADQFQQQVRIMCNLPPADTKLLSPCCMANILGDVWQEDGGEPDWLPLQS
                     RPNAHLHLYGKKTAQKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            23886..24368
                     /locus_tag="ACFB64_000779"
     CDS             23886..24368
                     /locus_tag="ACFB64_000779"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC1209176.1"
                     /translation="MSLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LRQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            24474..25522
                     /locus_tag="ACFB64_000780"
                     /pseudo
     CDS             24474..25522
                     /locus_tag="ACFB64_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
     gene            26102..26707
                     /locus_tag="ACFB64_000781"
     CDS             26102..26707
                     /locus_tag="ACFB64_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC1209177.1"
                     /translation="MNTDFQKILKQLGKQAKKEAAEKQEAEKNKQKQEYDLDFSQAVG
                     QVSPLKNRQQYYAQSDKTPIKARPKDNRTDEENYFYIGSTYNDPPASFCKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYVEPREGNDGCVRILLKRKLRQQD"
     gene            26770..27132
                     /gene="ssrA"
                     /locus_tag="ACFB64_000782"
     tmRNA           26770..27132
                     /gene="ssrA"
                     /locus_tag="ACFB64_000782"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         7224 a         7542 c         6211 g         6439 t
ORIGIN      
        1 aaattccgtc tgaaacagtc ttcagacggc atttttattt gcaaaaaaat caagcattgt
       61 ccaaggggac gagaatcgta cggttgccgt tgtgttccgg tgcggacaca atgccttccg
      121 cctccatctg gtcaatcaga cgcgcggcgc ggttgtagcc gatacgcaag gcgcgctgta
      181 cgcccgaaat gctggctttg cgcgttttca ggacaacgga tacggcctcg tcgtacatcg
      241 gatcggtttc gccgtcgccg ctgcgcccga tgccgggcag ctcttcgctg ccgccgccgc
      301 tcaaaatatc gtcaacatag tccggctcgc caaactgctt cagatattcg accacgcggt
      361 gcacctcttc atccgaggca aacgcgccgt gaacgcgctg cggataggca gtacccggcg
      421 gcaggaacag catatcgccc tgaccgagca ggttttccgc gcccatttgg tcgagaatcg
      481 tgcggctgtc gattttgctg gacacttgga acgcgatacg cgtcgggatg ttcgccttaa
      541 tcagacccgt gatgacatcg acgctggggc gttgtgtggc aaggataagg tggatgcctg
      601 ccgcgcgggc tttttgggcg aggcgcgcaa tcagttcttc gattttcttg cctgccgtca
      661 tcatcaaatc ggcaaactca tcgaccacga ccacgataaa cggcagtttt tccaaaggtt
      721 cgggatcgtc gggcgtgagg ctgaacggat tgccgatttt ttctcccctt gctgcggctt
      781 cggcggtttt ttggttgaag cccgcaagat tgcgcacgcc cataaagctc atcaggcggt
      841 agcgtttttc catttcgtta acacaccagt tcagcgcgtt tgccgccagc ttcatatcgg
      901 taacgacagg ggcgagcagg tgcgtgatgc cttcgtaaat gctcaattcc agcattttcg
      961 gatcgatcat aatcatacgc acgtcttccg gcgcggcttt gaaaagcatg gacagaatca
     1021 tcgcgttgac acccaccgat ttgcccgaac cggtcgtgcc ggcaaccagc aaatgcggtg
     1081 ctttgcccaa gtcggttacg acgggctgtc cggtgatgtc ctgaccgagc gcgagcgtca
     1141 gcttggattt ggattcggca aactcgggcg aattgaaaat ttcgctcagg cgtatcattt
     1201 ggcgtttcgg gttcggaagt tccaaaccca tgcaggtttt gccggggatg gtttcgacaa
     1261 cgcggatgga agccacgccg agcgaacgcg ccaagtcttt ttccaaattc agaacggaat
     1321 tgccgcgcac gccgacatcg ggttcgattt cataacgcgt aatcacgggg ccggaataag
     1381 aatcgacaac cttgaccttg actttgaact ccgccaattt ttcttcgatg gtgatgctgt
     1441 tttccaacag ttcttcttcg gtttgcgtcg cctcgggatt gaacagcgga ggcagaagca
     1501 ggtcggttgt cggcaggtgt tcggataccg caccggtctc ttccgattgg aacgcccctt
     1561 catccgcttc cgtatccgat acccggcagg acgggcgttc agacggcaca tcttcaaacg
     1621 gacaaaccgc ctgactgtca tgcccgaacg cttcggtttc cgacagatat tgcccgcttg
     1681 accgctctgc cgcaccctca ctgccgtcat ctgcaatagc ggcggtttcc tcctgccaac
     1741 ctccattcaa aacatcagcg gcaagatggt cggtttcggc aatgcggctg cgttgcgcct
     1801 gctcgaatcc tgccggcggc tcataggtac ggttgtagat ttccgatacg ggaggcggcg
     1861 gcagaatatc gatttcgggt acggctacct ccggcacttc gggtggttcg accacccaag
     1921 catcgggcgc ggcaggctct tcaatatgga tatcggcagc ttcatcataa accggaccgc
     1981 cgtatcccac agacgaaacg gtttccgtga aaacattttc ggtttcagac ggcatttcga
     2041 catcccgttt cccgacaacc gtccgacttt ccggaatcag gcgcgaaatc cgcgcctccg
     2101 cagaaaccgt tgccttgttc ggttctttca aattgacgga aacgcggcgg cgggcatcgc
     2161 ggacggctgt gccgtctgaa acggactgcc cttgggaaac atccggggat tttgcctccg
     2221 cctgcccttt ctcctgccct gcatgacggc ggaacggcgc attccgtgca ttttcggctt
     2281 tgactgcaga aaaggcagac ggatgctgct tctcaaaata cggctcaaat ccgtaatccg
     2341 cagactcgga aaacgcttct ttgtccgcat caaacatacg ggaatacgtg cgttgaagca
     2401 cagggtcgtc caaaccgata atctgcagcc cttccatcgg ggtatcggac acgcgtactt
     2461 tggggacggc tgttccgttt ttctcaaatg catcgatata gcgttttttc gtttccctta
     2521 aagccgcgct gcttaaagca cgcgttgctt cttccaaagt gatttcctta aaaaccggac
     2581 ggacgggcga agttttgctt tcagactgca tcaacccttc agcaatactc cggtcgaatg
     2641 ggatgcggcg gttgtcgatt acggcagttg caatgtcttc cgtatcggca gcctcttcct
     2701 ccgcagcttc tgctgcttcc gtttccgctt cttccgtccc gtttccactg tctgaatacc
     2761 cgtcggatgc ggtcggaaca tccgcacggt ttgcagaagc aggcggaacg ggcctgtccg
     2821 gttcggattc gtgcgccaga tagtttcggc agaaacggac ggcaccgaca aacagccaat
     2881 acactgccgt tttgacggca tggaaaagca tcagggaaaa ctcgggaaaa tcgggcatac
     2941 cgtctttgat ttcaggcagc tccgcctgtt tttccccttt cttttcctgc cacgcagaaa
     3001 cctcgcgcat ccactcgcgt tcggattgtg cgcggacaaa aaacaggccg gcaagcgcaa
     3061 gcaacaaaat aacgatcaaa actatccaaa acatatgccg tttcccgcaa gacaaacgat
     3121 taaccgagtt tattttaacg tgtttccagc ccaaaaaaaa caaccggccg gcaattctgc
     3181 cgaaaccgat tcggctttcg gtttgccccc gccgacagta taatagccgc cccttccctt
     3241 aatctttgcg gcattaccat gattttccaa acagtctggt tttcagatgt ggtactgtcg
     3301 gtttcgtgga ttgtccttat cctgatactg gcagcttccg cgccgtcggc attccgttcg
     3361 cttgcccgct accgttccgc ccttcccctg tgtaccgtaa ttttttccgc agcatggtgt
     3421 ctgaacgcct ctgtcggcgg cggacagctt gcccagatga actaccacct gcttgccgtc
     3481 aatctggtta cactgatggt ggatacctcc gccgcccttt ggcttgccgc gctgctgatg
     3541 ctgccctact gcctgctgtt tgccggttct gccggcgcat atccgccgaa cgctttggtg
     3601 ctgatactgc ccgcgcttgt cgtcaaccgc ctgtcgcgta tgctggtcaa ccgtctgccg
     3661 cccaatattt tcatctttat tttcgtcaac ggctttctcg cttccgccgc cggcattctg
     3721 ctgaccgggc tggtgctgat cggtattttg gatgccgcaa acgcttttcc gtccgaaata
     3781 ttgtggacgg ccgccctgcc cgtctttatt ctgctggcgt gggcggaagc cttcctcagc
     3841 ggcatttcaa ctgccatttt cgtggcactg aagccccatt ggatcaacac tttcgacgac
     3901 aaccgctatc tgaagtccga gcgcggcata tggctgtagc cttcagacgg cataggcaga
     3961 caaaaccatg ctttccaaca tcctccccct ttccatcggt gcaatattcg gcacaacggc
     4021 gcgttggctg ctcaacctcg ccgtccccgc atcgttgtct cccgccacag gcaacctgtt
     4081 tgccaactgg acgggcgcgc tgctcatcgg catctttgcc gaaaccgtca gccatccgca
     4141 atggaagctg ctgctgatta ccggtttttt cggcagcctg accacgcttt ccggattttc
     4201 actggaaacc gtaaccctgc tccaatcgaa ccgtcccgct tcggcacttg ccaatatttt
     4261 cctacacacg gcaggttcgc tgctgctgac ttggctcggg ctgaagatag ggacggcagt
     4321 caaataaccg caaaaaattg cttcctttgg ttttgacata atctataaat tggcttcgct
     4381 tgtttaaata ctgcctgtgc caattatagt ggattaaatt taaatcagga caaggcggcg
     4441 agccgcagac agtacagata gtacggcaag gcgaggcaac gccgtaccgg tttaaattta
     4501 atccactata ccgttctcaa attcaaattg ggcttttagg tctataacaa agaaaaagcc
     4561 ttgggaggat aaacctacca aggctttttc ttaaaaaacg ctctatcact gtgcctaaaa
     4621 atgcaaaaaa gtaaccgtca taatcctttt gccaaaatca atatcatcat ccgatgaatg
     4681 aacggacggt atataagaaa gcgttaataa actcataaac cagtaagaaa aatcagtaac
     4741 gaagatattc attattaccc attcttaaaa attatcacgt tcgtttgggt aattatcata
     4801 cccttttccc tcttattact aaagcacgaa ttactttaaa gtacgcaccc agagacaagc
     4861 cgaaaaggca tatccgatcc gccaaacaaa atgcccgccg acttactccg gcgggcggtt
     4921 ccaacatcct gtcaatcctt catcagccag tattgcaaac cgattaatgt tttaccgtct
     4981 ttaatttcat cgtttgccaa tgcctgacgg acttcatctt tcgacatcag cacggtttcc
     5041 gttatttcat cttcatcatt agaaagcgta ctgcccaagc gcacgccttc cgcctcgaac
     5101 agatacattt tttcattgca aaaaccgacc gccgtataaa aactgtacag caggcgcacg
     5161 ctgtcggcaa cataaggcgt ttcctccgcc aattctcgca gcgcacacgc tgccatatcc
     5221 tcgcccgcca catccagctt gcccgcagga agttccaatg tcgcctgatt tgccgcataa
     5281 cgccactgcc gcaccaaaac gaccttttcg tcatccgtta ccgccaacac gcacgccgcg
     5341 cccggatgcc ggatgacgat gcgctgccct tcattgccgt tgggcagcct gaccttatcc
     5401 ctgctgatac tgacgaaacc gccctcgtaa atggtttcgc cgcctaattt tacttccctc
     5461 aaatccactt catatctcct ttccgtttcc tattctgccg ttcaatcttc acgcttgccg
     5521 cgctcgattt ccactccaac ctcgcgcacg ccttccaaaa tacccggctt gacgattttc
     5581 acgtgcaccc acaccgcgcc gaaatatccc aaaaccaaat cggcaatata ttccgccaac
     5641 gcttccaaaa gcaggaaatc ctgttctttc agatgtcggc gcagcgtttc gcatacctcg
     5701 gcataatgca ccgtattggc aatatcgtcg tccgaacccg ctttctcggg aacgccgatg
     5761 tccaaatcga caatcagggt ctgcaaccgt tcgcgttccc agccgtacac gccgataagc
     5821 gtatccgcct tcatgccgtg caaaaagatt ttatccataa tccgccccgt tttttgctaa
     5881 aataaccgca ccattctaag taaagtaccg caaatgttca acataccggc cgtcgccgtt
     5941 tcctatttaa tcggttcact ttctttcgcc gtcatcgttt ccaagtatta cgggatggac
     6001 gacccgcgca cctacggatc gggcaatccc ggcgcgacca atgttttacg cagcggcaaa
     6061 aaaaaggcgg ccgcgctgac gctcttgggc gatgccgcca aaggtttggt tgccgttttg
     6121 cttgcacgcg tgcttcaaga accgctcggt ttatccgaca gcgcaatcgc cgccgtcgca
     6181 ctcgccgcgc tggtcgggca tatgtggccg gtgtttttcg gatttaaggg cggcaaaggc
     6241 gtggcaacgg cattgggcgt gcttctggca ctctctcctg caactgcctt ggtctgcgcg
     6301 ttgatttggc ttgtgatggc attcggcttc aaagtatcct cccttgccgc gctggtcgcc
     6361 acaaccgccg ccccccttgc cgcactgttt tttatgccgc atacttcttg gattttcgca
     6421 accctcgcaa tcgccatatt ggtgttgctc cgccataaga gcaacatcct caacctgatt
     6481 aaaggcaaag aaagcaaaat cggcgaaaaa cgctgattcg gcatttgttt tccattacgt
     6541 catgccgtct gaaaccatgt tcccgcgttc agacggcatt tccgcacaaa cggctttcat
     6601 acataccatg ctcagttaca gacacgcatt tcacgccggc aaccacgccg atatgctcaa
     6661 acacttcacc ttgtttttag tgctgcaata tttcaaccgc aaagacaagc cctactggta
     6721 catcgacacg cacggcggcg cgggtttgta caatctcgaa ggcagcgagg cgcagaaagt
     6781 cggcgaatac cggcaaggca tcgccctgct ccgacaggcg caaaacctgc ctgccgaact
     6841 ctctgacttt gccgcgcaca tacaaaaaat cctaccctcg cccgaacttt actgcggctc
     6901 tccgtggctg gcgcaatcgc tgatccgcat cggcgacaaa ttgcgcctgt ttgagctgca
     6961 ccctgccgac tttgtccatc tgcaaaacaa tatgggcgaa gcgggattgg gcaaacgcgg
     7021 acaagtgttg cgcgaagacg gttacaaagg gctgatttcc ctgctgccgc ccccgccgcg
     7081 acgcgctgtt attctcatcg acccgcccta tgaggaaaaa caggactacc ggcgtgtaac
     7141 ggaaacgctg aaagccgctt taaaacggtt tgagtccggc tgttatctca tttggtatcc
     7201 ctgcctcagc cgcgaagaaa gccgcaagct gccggaagaa ttgaaaaaac tcctgcccga
     7261 caactacctt tacgccgaac tgcacgtcca cgcgccgaaa accgacggtt tcggtatgca
     7321 cggcagcgga atgttcgtga ttaatccccc ttacctttta gccgaacaat tggcggcaaa
     7381 cctccccgcc ctgacacggc tgctggcgca agacgaaggc gcgcgctacc tgttggacaa
     7441 caaaatccgc tgaccccgat gccgtctgaa acccttcaga cggcatcttc cgtcaaacaa
     7501 acaaaaccgg gcgatacaca ctccatatcg tccggttctg cataaaacca gtattcaaaa
     7561 atcagaacac gcttttcatt acacccacgg taccaaccac accgttcagc agcatcagac
     7621 caccggcagc cgtacccata ccttgacgga ttgcgtcatg cttattggca cgtttttcac
     7681 gttccaaagc caactcctca gaaacatttt tacgttgacg gcggtgttgc gccagttccg
     7741 cacggctaat tcgcgcttcg gcacggttac gaccagcctt gattacggcc gaatcgtcgc
     7801 cgatagcata gttggctaca gaattttcag aagcgcaggc agcaagcaaa acggcacaca
     7861 cagcaacaac ggaaatattt ttcaagtttt tcatacctgt cctattatct caaaataaag
     7921 aaaaaactaa tccaactgga gttttatcgg ataaaataaa tattgggtga attttcgttt
     7981 gcctttttga tggcgcgtgc cgcatccatc atttcttccc ggcgttcttt acggtattcg
     8041 gcacgctcgg cacgctcgct tatctgaatg gcacgacttt tcgcctgtat ttccgctgca
     8101 gattcttgag ttacaaaacg gcggtctgcg taagtacatg ccgtcaccaa catcgcagcc
     8161 accgacaaca aaatcagttt cttcattttc atctacctat atttaagttt atttgtaaaa
     8221 tatgttatca ctatatatat atataatgtc aatatttttt aaaaataaat ttggcatctt
     8281 gtacttctcg atccgcctgc cgcgttccga cttgccccgc gccgtttttt tccactacaa
     8341 tcccacgcga tatccggcgg aacgcaccaa aggaacgccc cgcagcaatc tgccgccttt
     8401 cagacggcac aaacctttcc gaaacgagca aaccatggac acactgaccc gattcctccc
     8461 cgaacacctg caacaaaacc aactgcccga agcactcggc ggcgttttgc tgtccgtcgt
     8521 ttccgcctgt accgaaatca acgccaaagt ccgcctcggc gcacttgccg gcgtattggg
     8581 tatggcgggc acgggcaata ttcagggtga agaccagaaa aaactggatg ttatcgccaa
     8641 caacattatg attgacacac tcaaagccaa ccctgccgtt gccggtttgg caagcgagga
     8701 agaagacact ttcgtaagtg ccggtgaaaa cggacgctat ctcgtcctat tcgacccttt
     8761 agacggatcg tccaatattg atgtcaacat ttccgtcggt acgattttct ccattctcgc
     8821 caaacccgaa ggcgcattgg caaccgaatc attccttcaa acgggcagac agcagcttgc
     8881 cgcaggctat gtcctctacg ggcctcaaac ccagctcgta ttcacattcg gacacggcgt
     8941 gtacgtattt acactcaatg ccgaaaacga atttgtgctg accaaagaaa acccgaaagt
     9001 acccgaaagt accaaagaat tcgccatcaa tatgtctaac cgccgccact ggctaccccc
     9061 cgttcaacaa tacgtcgacg agctcttggc gggcgaaacc ggtacgcgcg gcaaaaacta
     9121 caatatgcgc tgggtagcca gtatggttgc cgaaatccac cgcatcctga tgcgcggcgg
     9181 cgtgttcatg tatctgcaag acaaacgcga cccgtccaaa cccggcaaac tgcgcctgat
     9241 gtacgaagcc aaccctatgg ccctgatact cgaacaggcg ggcgcatcgg caagcaacgc
     9301 atatcaagcc atgctcgata tacagcccga aagcctgcac caacgcgtcg ccgtcattat
     9361 gggcagcagc gaagaagtgg attacctcaa ccgtctgcat tcaaaatagg cagagaccgt
     9421 actgataaag accggcggca gcgtgaatcc gatactgcgc cgatatttca agcataccct
     9481 ccggaaaaac agaaaactgc ctttccgaac gataaaaata ccgtctgaaa aattttcaga
     9541 cggtatttta aaaattaaag ctcaaattta tttgcccgcc gctttttgca ggatggcgta
     9601 caactcgtct ttcagtttca atttggcttt tttcagctca tcgatggttt ccgcaccgct
     9661 ggtaaccgga ttgttggcca gaccggtaat tttatcgtcc agctcgttat gctcgtcgaa
     9721 cagacgggcg aagcgggaat tttcctgttt caatttggaa atcaaatcac ggtattctgg
     9781 gaacataatc gctttcctct cttggtttac ggttaaaaag tcaaattgaa tctttacatt
     9841 aattataaca aagataatat ttttatccag tgttttgtta tacacggcaa caccgtccgc
     9901 agaaaaccgc cccaccgtcc gataaaatcg ttgacaatct tccgctttac gcataaattt
     9961 tcagcccttc ctactgcata tgaaaggcac gccccagttt ccccaatgtg gattttcagc
    10021 gcgcgcagta gaggtgccga cccaaattgg cagacccttt gcttttgtta atatcctaga
    10081 aaatccagaa attcgcagta ctctaccact catcgccaat tggccaacct ttccacggct
    10141 ttgggttaat ggtgagttaa tcggtggtag tgatattatc ctacaaatgt accaatcagg
    10201 tgagctaaaa ccactcattg aagagcatag cccaaaggtc tgattactta atatcttaaa
    10261 ataaatatca aaaaagactg ctgatgacat tagcggtctt tttttgatgg tcaagattga
    10321 tagtttggat tgaattattt agccatatta catacaataa tcagcatgct ttgtatgaat
    10381 tatcaatcaa actcaggcga aggagtgctt gtggctaaaa catatttatt gactgcattg
    10441 ataatgtcta tggtaatctc cggatgtcaa gtcatccatg ccaatcaagg taaggttaat
    10501 actaattctg ctgtcatcgc aggtgcagac gctcacacgc ctgaacatgt aacgggactg
    10561 accgaacaaa agcaggtgat tgcaagtgat tttatagtag cgtcagccaa tccattagca
    10621 acacaagctg gctatgatat cttaaagcaa ggcggtagcg ctgcagatgc gatggtggcg
    10681 gtgcagacga cactaagctt ggtagagcca cagtcgtcag gcttgggcgg tggtgcattt
    10741 gtgttgtatt gggacaatac cgccaaaaca ttgaccacat ttgatgggcg tgagacggca
    10801 ccgatgcgtg cgacgccaga attatttttg gataaagatg gttaaccatt gaaatttatg
    10861 gaagcggtgg tcgctcggta ggtacgcctg ctatccctaa actgacggag acaatacatc
    10921 agcgatacgg tgtattgcct tggggaaaat tatttgatac gccgatccac ttggcaaaac
    10981 aaggctttga ggtgtcgcca aggcttgcca tctcggttga gcaaaatcag cagcatttgg
    11041 cacgctatcc aaaaacagcc gcttattttt tgccgaatgg tgtgccgcta caagcaggca
    11101 gcttgctgaa aaatttagaa tttgctgaca gtgttcaggc gttagcagct caaggtgcaa
    11161 aagctctgca tactggtaaa tatgcccaaa atattgtttc agttgtccaa aatgctaagg
    11221 ataaccccgg tcaattatcc ttgcaaaatt tatctgatta tcaagtggtg gaacgcccgc
    11281 ctgtttgtgt gacttatcgt atttatgaag tatgcggtat gggtgcacca agctcaggtg
    11341 ggattgctgt gggtcagatt ttggggattt taaatgaatt ttcacccaat cgggtggggt
    11401 atgatgctga aggtttgcgt ctcttgggcg atgcttctag gcttgcgttt gcggatcgtg
    11461 atgtatattt gggcgaccct gattttgtac cagtacccat tcgccagttg atttctaaag
    11521 actacctaaa acatcgcagc cagctgcttg agcagtcgga taaggcattg cctagcgtat
    11581 ctgcaggcga ttttattcat gagtgggcgt cctcacaagc gattgagtta ccttctacca
    11641 gtcatatatc aattgtggat aaggcgggca atgtgctatc aatgacgact tccattgaaa
    11701 atgcgtttgg ctcaactttg atggcaaatg gctacctgct gaataatgag ctgacagact
    11761 ttagttttga gccaataaag cagggcaaac aggtcgccaa tcgtgtcgag cctggcaaga
    11821 gaccacgctc ctcaatgaca ccaaccatcg tatttaaagc aggcaagcct tatatggcaa
    11881 tcggctctcc aggtggtagc cgtatcattg gctatgtcgc taagacgatt gtggcacata
    11941 gtgactggaa tatggatatc caagatgcca tcagtgcacc caatttatta aatcgctttg
    12001 gtagctatga actagaaacg ggtacaaccg ccgtccagtg gcaacaaaca ttaaatgatt
    12061 tgggctataa gactgatgtg cgtgagctaa attcaggtgt tcaagccatt attatcgagc
    12121 caagcaggct ggttggtggt gtcgatccac gccgagaagg tcgagtgacg ggtgattaga
    12181 ggtgaagctt gtattttgtg aatttgaccg cattaagtta attattaaaa tagggggaaa
    12241 ttgtgagtaa tcaaacaaaa atacaagcca tcaagcaagc ttctgagcag atcttggcaa
    12301 tttgtgagac gccaaacaca gcattacaag ccattcatct gattttaaga catggcgggg
    12361 caggaagaat tgtcttggca agtggtgtat cagcgggtta tggctgataa agatgtggtt
    12421 ggtgctggtt atttgattga ttttgcacaa actgccgaaa atttaccatt tgatgtattg
    12481 cctttgatta gccttgtgct aaacaaaggt gatgagacgc taaaaaccgg catgttaaat
    12541 aaattacctg ataatgccaa agaaaattta cgcattatgg gctatctgcc ttaggttttg
    12601 aagtattttt ttatacgaaa aacgctcatg ataccctgat tacacaaagg agccgagaat
    12661 atgttttcaa aaagcgttac cctcgcacaa tatgaccccg atttggcagc agccattgcc
    12721 caagaagacc gacgccagca agaccacgtc gagctgattg cctctgaaaa ctacgtcagc
    12781 tgcgccgtga tggaggcaca aggttcgcaa ttgaccaaca aatacgccga aggctatccc
    12841 gcaaaacgct actacggcgg ctgcgaatac gtcgatattg tcgaacaatt ggcaatcgac
    12901 cgcgtaaaag aactgtttgg cgcagcctat gccaacgttc aaccgcactc cggttcccaa
    12961 gccaaccaag ccgtatatgc ttccgtttta aaaccgggcg acaccatttt gggtatgtct
    13021 ctggcgcacg gcggacacct gacccacggc gcgagcgtta atatttccgg caaactctac
    13081 aatgccgtta cttatggctt ggatgaaaac gaagtcctcg attatgccga agtcgaacgc
    13141 ctcgcgctcg aacacaaacc caaaatgatt gtggcgggcg cgtctgccta cgcgttgcaa
    13201 atcgactggg caaaattccg cgaaatcgcc gataaagtcg gcgcatacct ctttgtcgac
    13261 atggcgcact atgccggact ggttgccggc ggcgaatatc ctaaccccgt gccattctgc
    13321 gacttcgtaa ccactaccac ccacaaaacc ctacgcggcc ctcgcggcgg tgtgattttg
    13381 tgccgtgaca atacccacga aaaagcgctg aactcttcca tcttcccaag cctgcaaggc
    13441 ggtccgttga tgcacgttat cgccgccaaa gccgtagcgt ttaaagaagc gttgcaaccc
    13501 gagttcaaac aatacgcaaa acaagtgaaa atcaatgctg ccgtcatggc ggaagagttg
    13561 gttaaacgcg gtttgcgcat cgtttccggc cgcaccgaaa gccacgtttt cctcgtcgac
    13621 ctgcaaccga tgaaaatcac cggcaaagcc gccgaagccg ctttgggcaa agcgcacatc
    13681 accgtcaaca aaaacgccat cccgaacgat ccggaaaaac cgttcgttac ctccggcatc
    13741 cgcatcggtt ccgccgccat gaccactcgc ggctttgacg aaaccgacgc tcgcgtattg
    13801 tcgaacttgg ttgccgatgt attggctaat cccgaagacg aagccaacct cgccaaagtc
    13861 cgcggacaag tgactgcctt gtgcgacaaa taccccgttt acggaaacta aacgcccgat
    13921 gtttccatac aaaatgccgt ctgaacattt tccagacggc attttttgtt ttcggtaaca
    13981 ttgctatttt ttctcttcca gcaaaatgac caactttttc aatactgcga tttcactatc
    14041 tttctgcctg atcaattcat cctttgcatc cagcagcgac ctcaattttt cattttcaaa
    14101 caccagcatt tcatcagagc cgataatatt atgatggtta ttggtattgt cgccgataga
    14161 gaagaaaaac gatcggttct gctccgccag cagctcaacc acatcgatat tgaaaatctg
    14221 ggcaatctgg cgcagcttgt cgagattgat accgcttttc ccgcgttcta ttttcgaata
    14281 cccgttaacc gacatttcca atttttccgc catctcttcc tgcgtccact gattgacttc
    14341 ccttaacgtc cgaatcttgt catgcacttc catcctgccc tccaaggtta attttcacca
    14401 ctcaaacaac cgtaaccggt atcttcagtt gatggtcgga tattttaaaa ataaataata
    14461 taacacaata gtattttgat aaaaatttac ctaacgaggt acagacatgt attcagaaaa
    14521 caagcctaaa cgcaccgtaa aaatgaaaaa atactggcag cctgcaccct ttccctgtcc
    14581 ctttctgcct gtacttcgac tcaagtaact tcttttgtcg agggactctc cgagggactc
    14641 gtagatgtca gcgaagcctt tatcacaaca aaggcagaat catattctgc cccgtctctt
    14701 caggataagc aaattttcat aaatcgcgtc tcagaacgag cacaaaaaaa ttacaaagaa
    14761 agaaggaaaa agaaagaagc agacttacta aattcagcat cgtcattaaa tgaatcaaca
    14821 tcagaagccc tatctgccct taaaaacgct atcgtaattc ctaaacgccc taaacgctcc
    14881 ttgggcaaaa aatataaaaa cggttttcat cctgtaaata cgtgtgcgga tgtggaagct
    14941 cggtgggcaa acggagcaat gagagatgtt agggacatag aaaggctaat aagccgaaac
    15001 gtccctttac gtcgtatcgg agacgccatt caaaacatgt gcatggaaac caatgtgtcc
    15061 attaggatgt atcgccagga gtgtcgttca gaatcgtcag atttttatga cgaaatgctt
    15121 gagcatcacg agaatttcat aggatcctcc aaatcattcg catcatcact tcttcccctt
    15181 agcgttacaa actttacaac agacatggcg tgtactcccg aattttaatc taatcacact
    15241 ccacaactcc ctatcaatat aactgctact tctctgagta gtatcaacca acccttaaca
    15301 aaggactaaa aaatgaaaaa agcactgctt gcactgacta ttgccgccat ctccggtact
    15361 gctatggccc agttgcccga ctttctgggt aaaggcgaat ataccgtccg tacagacatc
    15421 tccaaacaaa cgctgaaaaa tgccgatttg aaagaaaaac acaaagtaca aaaaaacatc
    15481 ggtttccgtg ccgatatgcc gtttgacgat attcaccacg gcatgcgttt cgaagtatca
    15541 cacagccgag acaaaaaaga catgtacgtt gtgaccgaaa gtactactaa accattcggc
    15601 aaagacgtta aagaaaaacg caccgacgtg tatgccggtt acacctacac ccaaccgctc
    15661 agtgaagcca ccaaactgcg tgccggttta ggtttgggct atgaaaaata caaagatgcg
    15721 gtagctaatg agaaaggaac agtcagcacc gagcgcgaag ccttctatac caaagcgcac
    15781 gccgacctga cttccgattt gggcggcggc tggtatctga atccttgggc agaggttaag
    15841 gttgacctgg acgccaaatt gaaacacaac gcaactgtag ccggtgtctc agccgacatt
    15901 aacgcaaaaa cacgcggatg gggcgtgggc gtaggtgcga atatcggcaa acaaattacc
    15961 gataccgtcg gcatcgaagc cggcccgttc tacaaacacc gtcacttcaa agcatccggc
    16021 agcttcgttt tagacggcgg gaacatccga gtcgatccta ccaaaatcaa cgaatacggt
    16081 gttcgtgtcg gcgtgaaatt ctaatcgcgg cgatatgccc cagacacaaa accgcaagcc
    16141 ttttggtttg cggtttttga tgttgtagca gaccctaata ccgtctgaaa cttttcagac
    16201 ggcatttttc caatcggtta aaataccgcc tcgacattgt gttttggaaa acccgtgctt
    16261 gcttctgtga ttgattttat cctgcatatc gaccaacacc tgctcagcct gtcggcgcaa
    16321 tacggtgtgt ggatttatgc gattctgttt ttgattgttt tttgcgaaac cggtctgatt
    16381 gttacgccct tcctgcccgg cgattcgctc ttgtttgccg ccggcgggat tgccgcgctg
    16441 ggcggtatgg atattcattt gatggttgca ttattaagcc ttgccgccat actcggcgac
    16501 gcgctcaatt ttactgtcgg caaatatttc ggcggcaggc tgttcgccaa ccccgattct
    16561 aaaatcttcc ggtgcgaata cctcggcaaa acccgccgtt tttacgaaaa acacggcggc
    16621 aaaacgatta tcatcgcgcg cttcgtgccc atcgtccgca ccttcgcccc cttcgtcgcc
    16681 ggtatgggaa aaatgcacta tgccaaattt atccgctaca acatcatcgg cggcttgttg
    16741 tgggttatct tgttttctta cgcgggctat ttctttgcca acttccccgt cgtaaaaaac
    16801 aatctgggct tggtgatggg cggcatcatt attgtttccg tcctgcccgg catcatcgaa
    16861 atcgcccgcg ccaaacttgc ggcaaaatcc gaacgttaat tgttggaaat tgacattatg
    16921 aatatattat ccgtagaaaa cgcttccttt gccgtcggac atgtcgccct gctcgacaaa
    16981 acgtccttcc aactcgacag cggcgaaaaa gtcggcttga tcggacgcaa cggcgcaggc
    17041 aagtcttcgt ttttaaaaat cctcaccggc gtgcaaaaac tcgacgacgg gcagattatt
    17101 gttcaaaaca atctcaaaat cgtttatgtg ccgcaggagt ccttttttga taaggacgca
    17161 accgtatttg atactgttgc cgaaggtttg ggcgaaattt gcgatttatt gcgccgttat
    17221 catcatgtca gccatgagtt ggaaaatggt tcgagtgagc tcttattgaa agagcttaac
    17281 gaattgcaac ttgaaatcga agcgaaggac ggttggaaac tggatgcggc agtcaagcag
    17341 actttggggg aactcggttt gccggaaaac gaaaaaatcg gcaacctttc cggcggtcag
    17401 aaaaaacgcg tcgccttagc gcaggcttgg gtacagaagc ccgatgtatt gttactggac
    17461 gaaccgacca accatttgga tatcgacgcg attatttggt tggaaaacct gctcaaagcg
    17521 tttgaaggca gcttggtcgt gattacccac gaccgccgtt ttttggacaa tatcgccacg
    17581 cgcattgtcg aactcgatcg cggtattttg cgctcctatc ccggctcgtt ctccaaatac
    17641 agcgagaaaa aagcgcaaga gttggcggtt gaagccgaac acaaccgcct cttcgacaaa
    17701 ttccacgcgc aggaagaggc gtggatacgc aaaggcatcg aagcgcgccg tacccgcaac
    17761 gaaggccgcg tgcgccgttt ggaagaactg cgccgccagc gtgccgaacg ccgcaatgta
    17821 caaggacagg tcaacttcaa actcgacagc ggcaagaaaa gcggcaaaat catcgccgaa
    17881 ctggaacacg cttcgtttgc ctatgacgac aaagttatca tggataaatt ttccgccatc
    17941 ttgcagcgcg gcgacaaaat cggcttaatc ggcccaaacg gtatcggcaa aaccaccttc
    18001 cttaagctga ttttgggcga attgcagccg acctacggca gaatccgtat cggcagcaag
    18061 caggaagtcg cctatttcga ccagttccgc agcgccttga atgaaaacga tactgtgttt
    18121 tacacgctcg gacagggcaa cgattacgtc gaagtcggcg gcaagaaaaa gcatgtgatg
    18181 agctatttgg aagatttcct gttcccccct gcccgcgcgc aaagtcctgt ttcttcactc
    18241 tccggcggcg aacgcaaccg ccttttgctg gcaaaactct ttactcgtcc cgccaatatt
    18301 ttggttttgg acgaaccgac caacgacttg gacatcgaca cccaagaatt gcttgaagat
    18361 ctgttgcgcg attaccaagg cacggtattc ctcgtctcgc acgaccgtat gttcctcgac
    18421 aatgtaatta cccaaagcat tgttttcgaa ggacaaggcc gtctgaaaga atacatcggc
    18481 ggctatcagg actatatcga cgcaaaatcg cgggaaaata aaattcagac ggcatccgca
    18541 cccaaagcat ccgatgtcga acccgccaaa gaaaaaccca aagccaaccg tacggtcaaa
    18601 ctttcctaca aagaacagcg cgaactcgac gccctgcccg acgaaatcgc cgccttggaa
    18661 accgaacagg cagaaatcaa cgcgcagctt tccgatcccg gaattttcaa agattacgaa
    18721 aaagcaggag cattgcaaaa ccgcgccgaa gaaatcgaaa tgctgctttt ggaaaagctg
    18781 gaacgctggg agctgctgga aaccaaacag aacggcaatg ccgtctgaaa tcaaacagga
    18841 agaaaaaaaa tgttcaaacg acctgaagaa atcatcgtcc tgatacttgc cgtcctctgg
    18901 attgccggta cgtattttct cgccgccctg tttggagcgg atgcttatac cgtgttaaaa
    18961 atcactgccc ttaccctgct ttggtctgcc gcatcattcc tcctttggca aaaaaaacct
    19021 cagccggcat atctggccgc tgctgctcgg ctgcctgact gcctgttggt ggccgtatct
    19081 gaatcaatcg gtcgcacccg gttctttacc ttggcatgca tcatggacgt tcaaaaccat
    19141 cttagccctg attcccgcaa ccgcaggtta tctgtttaaa tggaaacaat accggcagcg
    19201 caaaacaaag ccgtaagccc gtcttttttc agacggcatc tccggtcaga ctgtcaacaa
    19261 ttccgataaa atgttatact cgcgcccgtt gccgcgccgt ccgaaaccgc cttttgggaa
    19321 agcagaaatg atcagcaaac aagaatacca agcccaagcc gcccaaggct acaaccgcat
    19381 cccgctcgtg caagaactcc ttgccgactt ggatacgccg ctttccctct atctcaaact
    19441 cgccaaccgc ccctatacct acctgctcga atccgttgtc ggcggcgaac gtttcggccg
    19501 ctattccttt atcggcctgc cttgcagcca ctatctcaaa gccggcggca aacacgtcga
    19561 tgtttatcaa aacggcgaaa tcgtcgaaca atacgacggc aatccgctac cctttatcga
    19621 agctttccac aaccgtttca aaacacccga aatcccaagc ctgccgcgct ttaccggcgg
    19681 cttggtcggc tacttcggtt acgaaaccgt ctacaatttc gaacacttcg cccaccgcct
    19741 gaaaaacacc gccaaagcca acccgctcgg cacgcccgat attttactga tgctgtcgca
    19801 agagttggcg gtaatcgaca atttgagcgg caaaatccac ctcatcgttt atgctgatcc
    19861 ctcgcagccc gacagctacg aacgcgcccg cgaacgcctc gaagacatcc gcacccagtt
    19921 gcgccaaagc tgcgccatcc cactttccct cggcagcaaa caaacccaag cagtcagcga
    19981 gttcggcgaa gaacctttca aagcctgcgt cgataaaatc aaagactaca ttttcgcagg
    20041 cgactgtatg caggtcgtcc ccagccaacg catgagcatg gaatttaccg acaatccgct
    20101 tgccctctac cgcgccctgc gcacgctcaa cccttcgcct tacctctttt actacgattt
    20161 cggcgatttc cacatcgtcg gttcctcgcc cgaaatcctc gtccgccgcg aacgcgacga
    20221 cgtcatcgtc cgccccatcg ccggcacgcg cctgcgcggc aaaacccccg ccgaagacct
    20281 tgccaacgaa caagatttgt taagcgacgc caaagaaatc gccgaacacg ttatgcttat
    20341 cgatttgggg cgcaacgatg tcggacgcat cagcaaaaca ggcgaagtca aagtcaccga
    20401 caaaatggtg attgaaaaat actcccatgt gatgcacatc gtttccaacg tcgaaggctg
    20461 tctgaaagag ggcgttacca atatggacat cctcgccgcc accttccctg ccggcacact
    20521 ctccggcgcg ccaaaagtcc gcgcgatgga aatcatcgaa gaaatcgaac cggagaaacg
    20581 cggcatctac ggcggcgccg tcggcgtatg gggcttcaac aacgatatgg atttggcaat
    20641 cgccatccgc accgccgtga ttaaaaacaa taccctcttc atccaaagcg gcgcgggcgt
    20701 ggttgccgac tccgacccaa cttccgaatg gcaggaaact caaaacaaag cccgcgcagt
    20761 ggttcgggcg gcgcagatgg tgcaggaagg gttggataaa taaatttcag acaaccttcg
    20821 cagatgaaaa gaaggtcgtc tgaaaatcac aaaacaacat cagagatact tacataaaaa
    20881 atcacaatgc aattcacatc attagaaatt tgtgccggag caggcggaca ggctttgggc
    20941 ttagaaaggg caggcttttc ccatgttgcg ttaatcgaaa tcgaaccgtc ggcttgtcaa
    21001 accctacgtt taaaccgtcc tgattggaac gttattgaag gagatgtccg cttgtttcag
    21061 ggcgaaggat atgacggtat tgatttattg gccggcggcg tgccttgccc tcctttttcc
    21121 aaagctggaa aacaactggg aaaagatgat gaacgcgatt tattccctga ggctattcgt
    21181 ctagctaagg aaactgaccc taaagccatc atgttagaaa atgtgcgggg gttacttgac
    21241 ccaaaatttg aaaattatcg caaccatatt acagaacaat ttgccaagtt gggttacctt
    21301 ggacaatgga aactacttta tgctgccgat tacggagtat cgcaactcag acccagggtt
    21361 ttatttgttg cgttgaaaaa cgaatacacc aattttttca aatggcctga accaaattcg
    21421 gaacaaccga aaaccgttgg agaactgctt tttgacttaa tgtcagaaaa taactggcag
    21481 ggggcgcata actggcgttt gaaggccgcg cagattgcac cgaccttggt cggcggttca
    21541 aaaaaacacg gcggtgctga cttggggcct acacgatcca aacgcgcatg ggcggagttg
    21601 ggtgtggatg gttcaggttt gtgggatagt gcgccgcctg aagacttttc gggaatgcct
    21661 agattgactg tccgaatgac ggcgcgtatc caaggatttc ctgataactg gcaatttttt
    21721 ggtaaaaaaa cgcctatgta ccgccaaatc ggcaacgctt ttcccccgcc ggtagccgaa
    21781 gcggtgggca ggcagattat caaagcctta aaaaaggaaa attgaatgaa tccgcttttt
    21841 acccaagaac gcaggatttt ccataagaaa ttattggatg gcaatattct tgcaaccaac
    21901 aatagaggcg tagtcagcaa tgcggatggg agcaataccc gaagtttcaa tatagccaag
    21961 gggattgccg acttactgca ttcggaaacg gtttcggaaa gattacccgg tcagacatcg
    22021 ggaaatgctt ttgaagcaat atgcagtgag tttgtccaat ctgcttttga gaaattgcag
    22081 catatcagac ccggcgactg gaatgttaag caggtcggtt ctcgcaaccg tctggaaatc
    22141 gcacgttatc agcaatacgc acatctgaca gccttagcca aggctgccga agaaaacccc
    22201 gaattggctg ccgccttggg cagtgattac accattactc ccgatattat cgttaccaga
    22261 aatttgattg cagatgcaga aatcaaccgt aatgaatttt tagttgatga aaatattgcc
    22321 acatatgcca gtttgcgtgc gggtaatggc aatatgccgc tgctccacgc cagtatctct
    22381 tgtaaatgga cgattcgcag cgacagggct caaaatgccc gttctgaagg attgaatttg
    22441 gttagaaaca gaaaaggcag actgccgcac attgttgtcg taacggcaga accaacgcca
    22501 agccgcattt catctattgc cttgggtaca ggggaaatag attgcgttta ccattttgcc
    22561 ttatatgagc tggaacaaat tttacaatca ttgaattatg aagacgcttt ggatttgttt
    22621 tacatcatgg tcaatggtaa acgattaaaa gatatttccg accttccctt agatttggca
    22681 gtttaaacag aattattcca ctatgaacac accccccatc cttcctcccg ccatgctcgg
    22741 catcctcggc ggcggacaat taggcagaat gtttgccgtt gccgctaaaa ccatgggcta
    22801 caaagtaacc gttctcgatc ccgacccgaa tgcgccggcg gcggaatttg ccgaccgcca
    22861 tttgtgcgcg ccgtttgacg accgggccgc gttggacgaa ttggcaaaat gcgcggcggt
    22921 tacgaccgaa tttgaaaacg tcaatgccga cgcgatgcgc tctctggcaa agcataccaa
    22981 cgtttccccc agcggcgact gcgtgtccat tgcacaaaac cgcattcagg aaaaagcgcg
    23041 gatacgcaaa gcaggcttgc aaaccgcgcc gtatcaggcg gtttgcaagg ccgaagacat
    23101 tactgaagca agcgcgcaat ttttgcccgg catcctgaaa acggctacgt tgggctacga
    23161 cggcaaaggt caaatccgcg tcaaaacgtt ggacgaactc aaagccgcgt ttgccgaaca
    23221 cggcggcgtg gattgcgttt tggaaaaaat ggtggacttg cgcggcgaga tttccgtgat
    23281 cgtatgccgt ctgaacgatg aaaacgtgca aaccttcgac cccgccgaaa acatccacga
    23341 aaacggcatc ttggcttatt ccatcgtccc cgcgcggctg agtgccgacg tgcagcaaca
    23401 ggcgcggcag acggcgcaac gcttggcgga cgaattggat tatgtcggcg tattggcggt
    23461 agaaatgttt gttgtcggcg acacacatga attgctcgtc aatgaaaccg ccccgcgcac
    23521 gcacaattcc ggccaccata cgatagatgc ctgcgccgca gaccagttcc aacagcaggt
    23581 acgcattatg tgcaacctgc cgcccgccga caccaaatta ttatcccctt gctgtatggc
    23641 gaatattttg ggcgacgttt ggcaggaaga tggcggcgaa ccggattggc tgccgttgca
    23701 aagccggccg aatgcacacc tgcacctata cggaaaaaaa accgcacaga aaggtcggaa
    23761 aatgggacac tttaccgttt tgaccaccga ttcggacacc gcatttcaag aagcaaaaaa
    23821 actgcatcag tccctataat ccgaccaacg gcacagatgc cgtctgaagc cgccgacata
    23881 ccgccatgag cctgctgaca ctgttacgcc ccgccaccgt gcaagactgc aaagacatct
    23941 tcaaagtcca cctgcactcc gtccaataca cctgcatcct cagctacaac gaacacgccc
    24001 taaaagtatg ggaaggcttg ttaaacacag aaagctacct gccgaccatt tccgatcccg
    24061 acaaagcact ttgggttgcc gaatacaaag gcaacataca gggatttttc caaatcgact
    24121 gccaagaagc acaactcgat gcgctttacg tccaccccct gttccacaat ctcggactgg
    24181 gcactgccct gctccgtcag gcagaaacca tcgcccacaa atccggtttg agctttctga
    24241 aactctacgc atctctcaac tccgtcccgt tttaccttct caaccgctac gaatcgctcg
    24301 gttcggcagt attgcagctt gaccccagca tcaaaatcaa atgcgaactg atgcgcaaac
    24361 acctctaatc ccgccttata accaaaactg ataatcttca aatcaaacat tatttccaca
    24421 agcgcagaca gccgtataaa gttgccccat ctaccaaccg acaggacaag catatgaaaa
    24481 cctacgcaca ggcactctat accgcagccc tgctcaccgc ctgcagcccc gcagccgatt
    24541 caaaccatcc gtccggacaa aatgccccgg ccaataccga atccgacgga aaaaacatta
    24601 ccctgctcaa tgcctcatac gatgtgacac ggtattttta caaagaatac aaccacttat
    24661 ttatcaaaac ataccaatcc gaacaccccg gcacatccgt cagcatccaa caatcccacg
    24721 gcggcttcag caaacaggca ttatccgtag ccaacggcct tcaagccgat gtcgtaacca
    24781 tgaaccaatc ttccgacatc gacctgctcg aaaaaaagga ctggtagaaa aaggctggca
    24841 acaagccctc cccgatcacg ccgcacccta caccagcact atggttttcc ttgtccgaaa
    24901 aaacaacccc aaacagatcc gcgattggaa cgaccttgcc aaagacggcg ctaacatcgt
    24961 catcgccaag acctcgggca acggacgcta cgccttcctc ggcgcatacg gttacggtct
    25021 gaaagccaac aacggcaacg agcaggaagc ccaaaaactc gtcgcatcca tcctcaaaaa
    25081 cacacccgtt tttgaaaacg gcggacgcgc cgccgccacc accttcacac aacgcaacat
    25141 cggcgacgta ctcatcactt ttgaaaacga agccaactac gtcagcaaaa aactgaccca
    25201 aggacagttt gaaatcgtct atccgagcta caccatttcc gccgaaagcc ccgttgccgt
    25261 cgtcaacagc gtcgtcgcga aaaaaggcac gcaaaaaacc gcccgcgcct atctcgaata
    25321 cctttggagc gaaccggcgc aagaactcgc cgcctcactc tacctgcgtc cccgcaaccc
    25381 cgaagtattg gcacgacaca aagccgactt ccccgactta gacaccttcc ctcccgaaga
    25441 aaaattcggc ggatgggaca acatcatgaa aacctacttc gccgacggcg gcgtattcga
    25501 ccgcctgacc gcacaaaaat gaccattatg ccgtctgaac cgcctttcag acggcatttc
    25561 acacatcaaa acagaaaacc gcacccaaaa tcatcatcac aagacacaac ggcaaaccgg
    25621 atacaaaacc caacaccgcc gccaaaatgc cgtctgaagc cgtcggcgca accatcctta
    25681 tctggggcgc gcctcatcaa aggggcgcat aactttcctt aaccatccct tttgacagcc
    25741 aaataaacag tgcttttgcc tgtcagaaac aaaaccaata ttttgcggtt cgtatcggaa
    25801 catttataaa acatcagatt tccgtgtttc gcgtttccgg caattcaaga ttagcaaaac
    25861 cattaacctg cttcgcaagg caggttttta aaactttatc aggcgttcga gcatatcggt
    25921 tcgaccgacc cgcatttccg gcaatcaaca ctgccgacgg ttgccgcatt tctttttgat
    25981 atacaatcac actttcaaaa aacatacgac ggcaacaaaa tcatcctccc cccgcttggc
    26041 aaacatccgg caaattaaaa ccccgcccga aacagccatc ggacaaataa caggaaaaaa
    26101 tatgaatacc gacttccaaa agatcctcaa acaactgggc aaacaggcca aaaaagaagc
    26161 ggcagaaaaa caggaagccg aaaaaaacaa gcaaaaacaa gaatatgatt tggatttttc
    26221 acaagcagtc ggacaagtct ccccattaaa aaaccggcag caatattatg cgcagtccga
    26281 caaaaccccc atcaaagccc gtcccaaaga caaccgcacc gacgaagaaa actacttcta
    26341 cataggcagc acatacaacg acccacccgc cagcttctgc aaaaacggac agggaaaaaa
    26401 cgacatccaa cgcctgaaaa acggatacta tcccgtcgtt accgatgtcg acctgcacgg
    26461 ctacacccaa gaagaagcac aaaaagtcct caacgaattc atcgcattca cccaaaaacg
    26521 cggcgtatgc ggcgaaatca tccacggcag cggattaggt tccaaaggct acaaacccgt
    26581 tctgaaaaac atgacccgaa actggctgat gcaacacccc gacgtactcg cctacgtcga
    26641 accccgggaa ggcaacgacg gctgcgtccg catcctgctc aaacgcaaat taaggcagca
    26701 agactgacaa atttctccga cagacttggc agcaccgcac aatatccgta taatttgcaa
    26761 accatttctg ggggcgacct tggtttcgac gggggttgcg aagcagatgc gggcataccg
    26821 gggtctcaga ttcccgtaaa acactgaatt caaatagtcg caaacgacga aacttacgct
    26881 ttagccgctt aaggctagcc gttgcagcag tcggtcaatg ggctgtgtgg cgaaagccac
    26941 cgcaacgtca tcttacattg actggtttcc agccgggtta cttggcagga aataagactt
    27001 aaggtaactg gtttccaaaa ggcctgttgg tcggcatgat ggaaataaga ttttcaaata
    27061 gacacaacta agtatgtaga acgctttgta gaggactttc ggacgggggt tcgattcccc
    27121 ccgcctccac catgccctat tttcagcagt atcctgcaac acaaaaaatc cttgaacttc
    27181 aaaagtttca aggctttttt attgccttga atatgatggc gtatgcttga gttttcgcaa
    27241 tttatggggt aacttttggg gtaacttttc aggaatcacc ctaaaaaata tcttaaaaat
    27301 acaaaagcca tcacgcattt tttaaaaagg ttacccaaaa cttaaatgaa gcctttattt
    27361 ataaggcttg cagctttcca aacaagttac ccccaaacgg ataaaaaatg ccgaag
//
LOCUS       DAXDXW010000021        26751 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXDXW010000021 DAXDXW010000000
VERSION     DAXDXW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26751
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.021"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(54..599)
                     /locus_tag="ACFB64_000783"
     CDS             complement(54..599)
                     /locus_tag="ACFB64_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209178.1"
                     /translation="MNTQISGMKKYLKFWEYKRLLRVRGIEDLEKEIKLKLVDFELLI
                     DEAQSFHEACQGLNLIYPIVREHLKLSNKSLAGLDLKKYYIPNMIFFKNVVSSSGRMS
                     RKKFFKWADISTALDDTNASLDERLLHMKVYFDCRQYFCRGRQIAGDLAELFSMKEIF
                     DEILRIENLDRKNLPSNIMVK"
     gene            complement(695..1744)
                     /locus_tag="ACFB64_000784"
     CDS             complement(695..1744)
                     /locus_tag="ACFB64_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcaB family transcriptional regulator"
                     /protein_id="HFC1209179.1"
                     /translation="MEFAKQRYIIKPGAIHVAQQFDLKVTTKQSPFSDGYDMARAALA
                     LGDKVNQKLTEDDEQFQQWEEFKAHELLLKQHLQKETGRKHHLVWGEYEILSSENDRF
                     KNIGSLTSSGDDGFFMHTKQGIRMWEGNNNKKNGPRWPGIRYGLTLSNELFGMAMSDN
                     PYAQSRLLQIEKQMSEIEKFFETVKKQVHAQIDSLAAAGMKITLIQNNNPVHIRLNVL
                     RAYGFKLVKLLRDYDSFVCAVKTLNIKGMMANKQCNDTLYNGGRMMRKLLNDLYIAVM
                     ETRAIKSIRRDSLLDPEQLSKLKSAVEQEILPRIPLSVWVYESQPSLVYIQRKMNQED
                     LNKLVDIVRDNGLSG"
     gene            complement(1936..3453)
                     /locus_tag="ACFB64_000785"
     CDS             complement(1936..3453)
                     /locus_tag="ACFB64_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209180.1"
                     /translation="MVTATKLPPPKVFNSELTQCWQALQGGRRVAIYRCLIDITGSLK
                     TAAMLSQLIYWTRVSKEVAERDGWIFKSIAQMEAETGLTVREQRTCKNKLLETNLIQT
                     CRKGVGAALAIKVNLDAIAELIAKSSGTDDQLALTLADLQNTSSLYFRKHFSKRIAYH
                     RDLVSITGCINSAVLLSCVLNDAVGLVSQNPHLQRHAFATLTAADWEERTSLSYKSQL
                     TARNRLKNLNLIYERNFLASRRIFTLVNGHDIVISIKKLLAGKQDDGFSPYNSKKREI
                     LSLAERAKCDWRKGPNGLDKGFQSGFEGDAPLFPSMTSGSVEREYSDVSNWNFKKGRS
                     GKFISVDWGSSELPNRQIIYYINYKGVYNYNYMTVEPNKPRSSEQDHVVVVDDVDLIY
                     PKLFNVQMRQQADLIFTKHLSKSSWALRQEILDEIQGQKKEVTSPLGLLINLCRCASQ
                     GNFTALVAPQVRFQREQDQKLQAYLEKQNNQKQDSLDAMDVELARNNLRKFYSKR"
     gene            complement(3453..4196)
                     /locus_tag="ACFB64_000786"
     CDS             complement(3453..4196)
                     /locus_tag="ACFB64_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209181.1"
                     /translation="MDFSTSYDYETGFVPSKNMSTDNFIRSLVALWEEPATYVRQFAQ
                     AVGSVSEGYFLSWFVEKCLPGEWVSLNNDLLFNEAGITAKQWRNIRNSLLQKGILLNR
                     RTVAPTESQFTLNDALFEDLVSKHGKSDVLDMVSPPISINKLHLKTLADRGLAFVSIL
                     YLSFLQSEAEYQDLSKRGDWSPWTHAAEKHVFYSTMLSRRQQERAIAELTSFGLIESC
                     VDGMPAARRCRYSLKMLADLTSEYLNGCV"
     gene            complement(4205..4810)
                     /locus_tag="ACFB64_000787"
     CDS             complement(4205..4810)
                     /locus_tag="ACFB64_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209182.1"
                     /translation="MRSKPLISNPIYRLFNTDPVYTNLALTRLASAPNTPRESSYNAL
                     VAREHEDVLNTLKRMNPKRIEKLASMCPDVVTYSIDLKVLKSKISEMEHYEQRFMEQM
                     QRLKWMLEHKASNLMIMNLCSLISTAEIKKLRIEMNIPVVKGRIVMPSIDVRVRVLKA
                     WSKSEKEPDLFIRYQLLIDEFPDYSLAQLHSIINDVKFFGV"
     gene            complement(4800..6290)
                     /locus_tag="ACFB64_000788"
     CDS             complement(4800..6290)
                     /locus_tag="ACFB64_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFC1209183.1"
                     /translation="MNLDQNKATLSLHIGTPAQTNMSFGQEDTPSRGMLMSLPISEID
                     FFDKNPRRLHDVSSYAAIKESIRSSGIQQPVHVTQRPGESKYVLAQGGNTRLKILCEL
                     LEETGDNRFRMMPCIYIPYSDETTLQVAHLVENELRAEMCFWDKACKYAEIRDIFQDE
                     SDSGKLSLRELEVMFSDKGLTVSHKTLGLFFFASDNLQELQEYAFKLSHAKVEEIKKL
                     HSTLFSISKSIQLDDSFQYLWSDVLEQWHNQYSATELDVHELYQFVLDRFKFEYGSDA
                     FSETENSSDKDKIETAAKQDVNLHQKDSDMEFVPAKVNGTKADAEDEPKEAEPITLEQ
                     TSKKLFSAVRKLLAKVSLSDCFKIHKDFPYGWYVEYPAFEHISASSAGNAIYAIDVLH
                     EEAGNVFTFLWKLSGIDTFMFDVGDSSNPLLTLKSDSKLRIAYEDPDKYDEYTNCGIG
                     NKENIIASLLNWQTNTSHEFHANIVEILSLSTQYNNLRKTGAKSEE"
     gene            complement(6287..7174)
                     /locus_tag="ACFB64_000789"
     CDS             complement(6287..7174)
                     /locus_tag="ACFB64_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC1209184.1"
                     /translation="MSAPVILSAAATKGGVGKTTLIANVSAVLADIGLRVLMIDCDVQ
                     PSLSKYYPISHRAPNGIVELLLGENTEEIIRSTISNTVFPNLDIVISNNISAFVETEV
                     TSRPDRAFLLKSKLHSQYIQNNYDVVLIDTQGAVGPLLDTAAFAATCLLCPIMPEVLS
                     AREFLTGTQELLKRLQKGEAMRIPMPQLRAIIYAQDRTNDAKFIASSIKDFFSNTLDD
                     RRKLLSVAVPAAKAYKEATTLRVPVHCHERKQLGKMLPAYEIMHRIVYEIFPGIEQKN
                     LRGSCFNDMASLLQKEVQS"
     gene            complement(7415..7597)
                     /locus_tag="ACFB64_000790"
     CDS             complement(7415..7597)
                     /locus_tag="ACFB64_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209185.1"
                     /translation="MGVCPDARCSAAGIARQIGNGGGIHGTLAFSLLFLSLGHIGMVA
                     YHHIKGESVLPLFLLI"
     gene            complement(7653..7787)
                     /locus_tag="ACFB64_000791"
     CDS             complement(7653..7787)
                     /locus_tag="ACFB64_000791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209186.1"
                     /translation="MKFKQFILATVLGATAFSAWADDSYQHIRAVRILLLILSVWCVG
                     "
     gene            complement(7969..8628)
                     /gene="ung"
                     /locus_tag="ACFB64_000792"
     CDS             complement(7969..8628)
                     /gene="ung"
                     /locus_tag="ACFB64_000792"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC1209187.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPEADVFNA
                     FRLTAFDRVKVVILGQDPYHGVGQAHGLAFSVRQGVRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGILLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSQHGIEPINWK
                     L"
     gene            8709..9116
                     /locus_tag="ACFB64_000793"
     CDS             8709..9116
                     /locus_tag="ACFB64_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC1209188.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDTGKYAEILRYTGGNRYEVFYRGT
                     HWQAQNTGQEVFEPGTRALIVRKEGNLLIIANP"
     gene            9131..10078
                     /locus_tag="ACFB64_000794"
     CDS             9131..10078
                     /locus_tag="ACFB64_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC1209189.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRAMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(10185..11255)
                     /locus_tag="ACFB64_000795"
     CDS             complement(10185..11255)
                     /locus_tag="ACFB64_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC1209190.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVYALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FIQNTLLPWLKNTIGGYVEIDQASIIAWFQAHTGELSNALKAWFPVLMKQGGNIVSSI
                     GNLLLPPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLMLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LAVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGELMGFVGMLAGLPLAA
                     VTLVLLREGAQKYFAGSFYRGR"
     gene            11440..11715
                     /locus_tag="ACFB64_000796"
     CDS             11440..11715
                     /locus_tag="ACFB64_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC1209191.1"
                     /translation="MTEQKNKASLIEFPCAFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            11717..12340
                     /gene="lipB"
                     /locus_tag="ACFB64_000797"
     CDS             11717..12340
                     /gene="lipB"
                     /locus_tag="ACFB64_000797"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC1209192.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGIYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTQ
                     INPCGYAGMEMTQIADFVQPCPAPDEVASKLTAHLETQLTPKADNNE"
     gene            12333..13316
                     /gene="lipA"
                     /locus_tag="ACFB64_000798"
     CDS             12333..13316
                     /gene="lipA"
                     /locus_tag="ACFB64_000798"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC1209193.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFTNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            13791..14255
                     /gene="bfr"
                     /locus_tag="ACFB64_000799"
     CDS             13791..14255
                     /gene="bfr"
                     /locus_tag="ACFB64_000799"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC1209194.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            14283..14756
                     /gene="bfr"
                     /locus_tag="ACFB64_000800"
     CDS             14283..14756
                     /gene="bfr"
                     /locus_tag="ACFB64_000800"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC1209195.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELAKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            15084..15404
                     /locus_tag="ACFB64_000801"
     CDS             15084..15404
                     /locus_tag="ACFB64_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1209196.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQVPS
                     ESGI"
     gene            15453..18011
                     /gene="glnD"
                     /locus_tag="ACFB64_000802"
     CDS             15453..18011
                     /gene="glnD"
                     /locus_tag="ACFB64_000802"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC1209197.1"
                     /translation="MPENLSSALETFKQQRNAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEHFQNSALCLMAVGGFGRGEPYPCSDVDLAVVSPAPLSDGIQEQIARFIQTLW
                     DCKLMPSVKSGSVDELCESVRDDITGDTAFLEARFLFGNRQTADELAEKMNVQRNVAA
                     FIEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLAANLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIRLHLNAKRAEDRLLFDLQPQVAESMGYQDENRRR
                     QSEELMRVFYRAVKTVKQLGGILTPMLRSRVSSTPVRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRRRFAGF
                     FRSGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAVQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSAVAQKEDIQDPGVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRCLAGNDGNPHALFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFEPPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSCNRRISRRSRYMPIAPSITITPEEDYPDRYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERVEDSFTVFSPDLKNPKIQSSLKQALLEQLA"
     gene            18158..19621
                     /gene="guaB"
                     /locus_tag="ACFB64_000803"
     CDS             18158..19621
                     /gene="guaB"
                     /locus_tag="ACFB64_000803"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC1209198.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTYKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTDERVKALVEAGADVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(19960..22335)
                     /gene="rnr"
                     /locus_tag="ACFB64_000804"
     CDS             complement(19960..22335)
                     /gene="rnr"
                     /locus_tag="ACFB64_000804"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC1209199.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLMPMDEGDFVLYERQMRGVMHGDTVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGKIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHRFSEACAKSAKKIPDHVRKSDLKGRVDL
                     CDLPLVTIDGETARDFDDAVFAEKVGRNYRLVVAIADVSHYVRPDDAIDADAQERSTS
                     VYFPRRMIPMLPENLSNGICSLNPDVERLCMVCDMVVTYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSDGIGNPHKAQIDTLYKLFKILQKKRLARGAVEFESVETQMIFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALAEQFKGRPDAELLQVMMLRSMQQAVYEPHCEGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNRKTYTPNKSWQALGVHTSFCERRADDAGRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGESGRRRKVKLSASAKPAG
                     AAGKGKSKTTAEKKTARCGKVRGRGVPAVAESGKKAKKPVPIKVKKRKGKS"
     gene            22424..22510
                     /locus_tag="ACFB64_000805"
     tRNA            22424..22510
                     /locus_tag="ACFB64_000805"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22458..22460,aa:Leu,seq:cag)
     gene            22549..22639
                     /locus_tag="ACFB64_000806"
     tRNA            22549..22639
                     /locus_tag="ACFB64_000806"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22583..22585,aa:Ser,seq:gga)
     gene            complement(23036..24847)
                     /gene="typA"
                     /locus_tag="ACFB64_000807"
     CDS             complement(23036..24847)
                     /gene="typA"
                     /locus_tag="ACFB64_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC1209200.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGCHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKYTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLRVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(24964..25854)
                     /locus_tag="ACFB64_000808"
     CDS             complement(24964..25854)
                     /locus_tag="ACFB64_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC1209201.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVRAGFNQDTLPFAVIAGFPEIMLDTADGLTKRQG
                     INAWTAGCGLGTDAAAVETVAAVLARNRDEAVVLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAILGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     SAGEIAPAARWLRNWITESM"
     gene            25919..26575
                     /locus_tag="ACFB64_000809"
     CDS             25919..26575
                     /locus_tag="ACFB64_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC1209202.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LLVLFLLVFRLCWGIWGSDTARFSRFVRGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLIRKIHLNFFKLLAVFSAVH
                     IAAVAAYRIFKKKNLVRPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
BASE COUNT         6983 a         7213 c         6075 g         6480 t
ORIGIN      
        1 aaaaaaacca tcacaaaagt gatggcaaat gatttttaag gctattaaat atcttactta
       61 accatgatat tactaggaag atttttgcga tccaaatttt caattcttaa aatttcatca
      121 aaaatttctt tcatcgaaaa caattctgcc aagtctccgg caatctgcct acctcggcaa
      181 aaatactgtc tgcaatcaaa ataaaccttc atatgtaata accgttcatc aaggctggca
      241 ttcgtatcat ccaacgccgt actaatatcg gcccatttaa aaaacttctt tctcgacata
      301 cggccactgg aagaaacaac atttttgaaa aaaatcatat tcggaatata gtattttttt
      361 aaatccaaac cagccaaact tttattactc aatttcagat gttctcttac tataggatat
      421 atgagattaa ggccctgaca tgcttcatga aacgactgag cttcatcaat taatagctcg
      481 aaatcaacca atttcaattt aatttctttt tctaaatcct caatccccct tacccttaaa
      541 agccttttgt attcccaaaa ttttaagtat tttttcatac ctgatatttg agtattcata
      601 gtattcatat cacatcataa aaaaatccat acaaacaagg gccaatcaat accatcttaa
      661 aactcgattg taaccccacg ttccaatttc acaactagcc cgataatcca ttgtcacgaa
      721 caatatcaac caacttattg agatcctcct gattcatttt tctctggata taaaccaaag
      781 agggttgtga ttcatacacc caaacagaca atggaattct aggcagaatc tcttgctcaa
      841 cagcagattt caacttcgac aactgctctg ggtcaagcag actgtcacgt cgaatgctct
      901 tgatagccct ggtctccatt accgcaatat ataaatcatt cagtagcttt ctcatcatcc
      961 gaccgccatt atacaacgtg tcattacatt gtttattagc catcatgcct ttgatattaa
     1021 gtgtcttaac ggcacataca aaactatcgt aatcccgcaa caatttaacc agtttgaaac
     1081 cataagcccg caatacattc aatctgatat gaacagggtt attgttctga atcaaggtta
     1141 tcttcatacc tgcagcggcc aaactatcaa tctgagcatg aacctgtttt ttcacagtct
     1201 caaaaaattt ctcaatttcg gacatctgtt tttcaatctg caataaccga ctttgggcat
     1261 aaggattatc ggacatagcc ataccaaaca actcgttact gagtgtcaga ccatatctga
     1321 ttcccggcca acgtgggccg ttcttcttgt tgttattgcc ctcccacata cggatacctt
     1381 gcttggtatg cataaaaaaa ccatcatcac cggaagaggt caggctgcca atatttttaa
     1441 accggtcgtt ctcagaagaa agaatttcat actcccccca aaccaagtga tgctttcgac
     1501 ctgtctcctt ttgtagatgt tgcttgagca gcaattcatg tgctttaaac tcttcccact
     1561 gttggaactg ttcgtcatcc tcagtcaact tttgattgac cttatccccc aaagccaaag
     1621 cagcacgagc catatcgtaa ccgtcactga aaggtgactg ttttgttgtg actttgagat
     1681 caaattgctg tgcaacatgg atcgcaccag gtttgatgat atatctttgt tttgcaaatt
     1741 ccatttttaa atccttataa gttattaaaa agagcttcat aaagcactgt tgttgcatat
     1801 cagaggctaa ttagtcatgt acaccatcat atctgtcctt tgccgatgac tgtaacgccc
     1861 cgttacatcc gcttcgaagt aatagattta ttcgtctttc aaaaattcat caaaaatatt
     1921 tttttaaatt aaattttatc ttttggagta gaactttctt aaattattgc gggcaagctc
     1981 gacatccatg gcatctaatg agtcctgttt ttggttattc tgcttttcaa gataagcctg
     2041 taatttctga tcctgctcac gctggaatct aacctgtggg gcgacaagag cagtaaagtt
     2101 accttgcgaa gcgcagcggc ataggttaat caaaagacca agggggctag taacttcttt
     2161 tttctgtccc tggatctcat ccaagatttc ctgcctcaaa gcccaggaac tttttgagag
     2221 atgttttgta aaaatcaaat ccgcctgttg acgcatttgc acattaaaaa gttttggata
     2281 aatcaaatca acatcatcaa ccactaccac atgatcttgt tcggaagaac gtggtttgtt
     2341 gggttcaaca gtcatgtagt tgtagttata aacccctttg taatttatat aataaataat
     2401 ctgccgattc ggcaattctg aacttcccca atcgacactt atgaatttcc cactacgacc
     2461 tttcttaaaa ttccaattcg acacatccga atattccctt tcgacacttc cggatgtcat
     2521 acttgggaat aggggggcat caccctcgaa accgctctga aaccctttat ccaatccatt
     2581 tggccctttc cgccaatcac atttggccct ttccgccaaa ctgagaattt cgcgtttttt
     2641 ggagttatat ggactaaagc cgtcgtcctg tttgccagct agaagttttt tgatggatat
     2701 aacaatatcg tgaccattta ccagggtaaa aatacggcgg ctggccaaga aatttctctc
     2761 ataaatgaga tttaaattct tcagcctatt gcgagctgtc aattgagatt tataagagag
     2821 agaggttctt tcctcccaat cagcagcagt caaagtagca aaggcatgac gttgtaaatg
     2881 tggattttga gaaaccaaac caaccgcatc atttaacaca cacgacaata atacagccga
     2941 gttgatacag cctgtaatag aaaccaggtc tcggtgataa gcaatccttt tagaaaagtg
     3001 ttttctaaaa tacagggaac ttgtgttctg taaatcagcc agcgtaagtg caagttgatc
     3061 gtcagtaccg ctcgatttag cgatcaactc agctatggca tccaagttca ctttgattgc
     3121 taaagcagcc cccacacctt tacggcatgt ttgaataagg tttgtctcaa gaagcttgtt
     3181 tttacaagtg cgctgctctc tcactgtcaa accagtttca gcttccatct gagcaataga
     3241 cttgaaaatc caaccatccc gttcggcaac ctctttgctt actcttgtcc aatagattaa
     3301 ctgagaaagc attgcagcag tcttcagaga gcctgtaata tcaattaaac atcgatagat
     3361 ggcaaccctt cgaccacctt gcaatgcttg ccagcattgt gtcagttcag agttaaatac
     3421 tttgggaggg ggtaatttgg tggcggtaac catcacacac atccgttcaa atattcagaa
     3481 gtaagatcag caagcatttt caagctgtat ctacaacgtc gcgctgctgg cataccatca
     3541 acacagctct caatcaaacc aaaagaggtt aattcggcaa tagctctttc ctgttgccgt
     3601 cttgacaaca ttgttgaata aaaaacgtgc ttttcagctg catgagtcca aggagaccag
     3661 tcaccacgct tactcaaatc ttgatattca gcttcacttt gaagaaacga caaatacaaa
     3721 atggatacaa aagcaagacc tctatcggct aatgtcttca ggtgaagttt attaatcgaa
     3781 atagggggcg aaaccatgtc aagtacatct gatttaccat gtttacttac caaatcctca
     3841 aaaagagcat cattcaatgt gaattgtgat tcggttggcg ctacggttct ccgattaagc
     3901 aaaatacctt tctgcagaag gctattgcga atattccgcc actgctttgc ggtaatacct
     3961 gcttcattaa acaacagatc attattcagg cttacccatt cgccaggcag acatttctcg
     4021 acaaaccaac tcaaaaaata tccctctgaa acagaaccga ctgcctgagc aaactgacgc
     4081 acatatgtgg ccggttcctc ccacaaggca accaagctac ggataaagtt atcggttgac
     4141 atgttcttag atggtacaaa accagtctca taatcatagg aagtagagaa atccatatac
     4201 atactcatac tccaaagaac tttacatcgt taattattga atgtaattgg gcaaggctat
     4261 aatcggggaa ttcatcaatc aatagctggt atctaataaa caaatctggc tctttttccg
     4321 acttactcca tgccttcaat accctgacgc gaacatcaat agatggcatc acaatgcgcc
     4381 ctttaacgac aggaatattc atttctatac gcaatttttt aatttccgcc gtagaaatta
     4441 atgaacatag attcataatc atcagattgc tggccttatg ttctaacatc catttaagac
     4501 gctgcatctg ttccataaac ctttgttcgt aatgttccat ttctgaaatt ttgcttttta
     4561 aaaccttcag atcaatggag taggtcacaa catcaggaca catggatgct aatttctcaa
     4621 tccgcttcgg gttcattcgt ttgagcgtgt tcaaaacatc ctcatgttca cgagcaacca
     4681 aagcgttata agaagattca cgcggcgtat ttggtgcgga tgccaacctt gtaagtgcaa
     4741 gattggtata aaccggatcc gtattaaaca gtctataaat gggattacta attaaaggct
     4801 tactcctcac tcttagctcc cgtttttctc aagttgttgt actgtgtgct cagagataat
     4861 atttctacga tattggcgtg aaattcatgg ctggtattag tttgccaatt cagtaaagag
     4921 gcaataatgt tctctttatt accaattccg cagttcgtat attcgtcgta cttatcgggg
     4981 tcctcgtatg caatacgaag cttagaatca cttttaagtg tcagcagggg attggaacta
     5041 tcaccaacat caaacataaa ggtgtcgatt ccactaagct tccataagaa tgtgaaaaca
     5101 tttcccgctt cttcatgcaa aacatcaata gcatagatgg catttccggc acttgatgct
     5161 gaaatatgct caaaagccgg atattcgaca taccagccgt atggaaagtc cttatgaatc
     5221 ttaaagcaat cggacagact cactttggct aacagtttac gaacagcaga aaacagtttt
     5281 ttgctggttt gctccaaagt aatcggttcg gcttctttcg gctcatcctc tgcatcagct
     5341 ttagtaccat ttactttggc tggcacaaat tccatatccg aatccttttg atgcaaattg
     5401 acatcttgtt tggcggctgt ttcaattttg tctttatcag aagaattttc tgtttcagaa
     5461 aaggcgtcag agccatattc aaatttaaat ctgtcgagca cgaactgata aagctcatgc
     5521 acgtctaact ctgttgcaga atattggtta tgccactgct ccagtacatc cgaccacaaa
     5581 tattggaaag aatcgtccaa ctgaattgat ttagaaattg agaatagggt agagtgaagt
     5641 tttttaattt cttctacttt tgcatgggaa agtttgaatg cgtattcttg cagctcttgt
     5701 aaattatcgg aggcaaaaaa gaataatcct aaagtcttat gagaaaccgt aaggccttta
     5761 tcagaaaaca tcacttccag ttcgcgcaaa gaaagtttcc ctgagtcaga ttcatcttgg
     5821 aaaatatcgc ggatttcggc atatttacag gccttatccc aaaaacacat ttcagcacgg
     5881 agttcatttt caacaagatg cgcaacctgc aatgtagttt cgtcagagta ggggatgtaa
     5941 atacaaggca tcatacggaa gcggttatca ccagtttctt ccaagagttc gcacaagatt
     6001 ttgagacggg tattaccacc ctgtgccagt acatatttac tttcaccagg tctctgggta
     6061 acgtgtacag gttgttgaat acctgaagaa cggatggact ctttgatggc cgcgtagctg
     6121 gaaacatcat gtaaacggcg agggtttttg tcaaagaaat cgatttcgct gattggcaac
     6181 gacattaaca tgccccgaga aggagtatcc tcttggccaa aactcatatt tgtctgtgca
     6241 ggggtaccga tgtgcaggct caaggttgct ttattttggt ccaaattcat gattgcacct
     6301 ccttttgcag caggctggcc atgtcattga aacagcttcc acgcaggttc ttctgttcga
     6361 taccagggaa aatctcataa acaatacggt gcatgatttc ataagcaggc agcatctttc
     6421 caagctgttt acgctcatga caatgaaccg ggactcttaa agttgtagct tccttatatg
     6481 ctttggcagc aggaactgct acgcttaaca atttacgtct gtcatctaga gtattgctaa
     6541 agaaatcttt gatagaagat gcgatgaatt tcgcatcatt ggtacgatct tgtgcgtaga
     6601 tgatggctcg taattgaggc atcgggatac gcattgcttc gcctttttgc agacgcttta
     6661 gaagttcttg cgtaccggta aggaactcac gggcactcaa aacttcgggc ataattgggc
     6721 aaagcaggca tgtagcagca aatgctgcgg tatctaagag aggaccaact gcaccctgag
     6781 tatcgattaa aaccacatcg tagttattct gtatgtattg ggaatgcagt ttgcttttga
     6841 gcaagaaggc acggtcggga cgagaggtta cttcagtctc gacaaaagca gaaatgttat
     6901 tggaaataac aatgtctaaa ttcggaaaaa cggtattgga aattgtcgag cggatgattt
     6961 cttcggtatt ttcaccgaga agtaattcaa caataccatt tggagcgcgg tggcttattg
     7021 gatagtattt tgacaatgat ggctgaacat cgcagtcaat cattaagaca cgcagtccga
     7081 tatcagcgag aacagcagag acattagcaa tgagtgttgt tttacctacg ccaccttttg
     7141 tagcggctgc agacaatatt acgggtgcgg acataatatt tccttatatt taattgattt
     7201 ataaagagaa aaacatccgc aatcgaacag gtttaaaata tgaaatttac atatttattc
     7261 tagttcgcat aatgtatatt atgttaaatt tataatggat tgaatagata tttttacaat
     7321 ctcattcaaa actcagacac ttaaaaaatt taatcagaac aaataaaaaa gcaaacaaaa
     7381 atatccatct ttcaaactca tattttgcaa taacctagat aagcaagaac aggggcagaa
     7441 cggattcgcc tttgatgtgg tggtaggcaa ccattccaat atgtcccagc gacaaaaaca
     7501 gcaatgaaaa tgccagcgtg ccgtgaattc ctccgccatt gccgatttga cgggcaatgc
     7561 ctgcggcgga acagcgagca tcagggcata cgcccaatgc ccaatttggt gacggcggtg
     7621 ttggcggggc ggtttttacg ttggcggatt ccctacccta cgcaccaaac agacaatatc
     7681 agcagcagga ttctgactgc acggatatgt tggtatgaat cgtccgccca agcggaaaag
     7741 gctgtcgcgc ccaaaacagt ggcaagaata aattgcttga atttcatgat gttgcttctt
     7801 ttgcacaaag cgttcggaat agtgaagccc gcagcctatg gctttgcggc agtcgcagct
     7861 ttacaagcgg tattgcagga aaccttaaaa cacgatcatt gcatcaagag ctgttcgaat
     7921 tccgccaaat gaacctgaca cgcaaacttt gacgcttgaa tacggcattc atagcttcca
     7981 gtttatcggt tcgataccgt gttgactcaa atagctgttt gcctgtgaaa aatggcggca
     8041 gccaaaaaaa ccgcgatatg ccgacagagg agacggatga ggcgcggtca gtatcaaatg
     8101 attttgactg tctatcagcc tccctttttg ttgtgcatac ccaccccaca acatgaagac
     8161 aaggtgcttg cggtgtgtcg caagctgcct gatgacggta tcggtaaagc gttcccagcc
     8221 taaaagggcg tgcgaatgcg cctgtcctgc acgcaccgtc aaaaccgtgt tcagaagcaa
     8281 tatgccctgc tccgcccacg ctgtcaggca gccgtgcgcg ggaatggaaa agccttcgat
     8341 gtcggtttcc aattctttgt aaatattgag taaagacggc ggtatgcgga caccctgccg
     8401 gacggaaaat gccaaaccgt gcgcctgccc taccccgtga tacggatctt gtccgagaat
     8461 gacgactttg acccggtcga acgctgtcag ccggaatgcg ttgaacacat cggcctccgg
     8521 cggatagata atttgccccg acaaacgttc ctgcctgacc gcatttaaaa tttcctgaaa
     8581 atacggctgc tgtttttcgc cgccgagtgc atcgtgccaa gtgtccatat ctgccctatc
     8641 ctttcaaatt cggctatact gctgatattg cgttattttg tcaaacgacg cgctttgtgg
     8701 ggaaatgtat gactgtatgg tttgttgccg ctgttgccgt cttaatcatc gaattattga
     8761 cgggaacggt ttatcttttg gttgtcagcg cggctttggc gggttcgggc attgcctacg
     8821 ggctgactgg cagcacgcct gccgccgtct tgaccgccgc actgctttcc gcgctgggca
     8881 tttggttcgt acatgccaaa accgccgtgg gaaaagttga aacggattca tatcaggatt
     8941 tggataccgg aaaatatgcc gaaatcctcc gatacacagg cggcaaccgt tacgaagttt
     9001 tttatcgcgg tacgcactgg caggcgcaaa atacggggca ggaagtgttt gaaccgggaa
     9061 cgcgcgccct catcgtccgc aaagaaggta accttcttat catcgcaaac ccttaacact
     9121 cggaggaatt atggaatttt tcattatctt gttggcagcc gtcgccgttt tcggcttcaa
     9181 atcctttgtc gtcatccccc agcaggaagt ccacgttgtc gaaaggctcg ggcgtttcca
     9241 tcgcgccctg acggccggtt tgaatatttt gattcccttt atcgaccgcg tcgcctaccg
     9301 ccattcgctg aaagaaatcc ctttagacgt acccagccag gtctgcatca cgcgcgataa
     9361 tacgcaattg actgttgacg gcatcatcta tttccaagta accgatccca aactcgcctc
     9421 atacggttcg agcaactaca ttatggcaat tacccagctt gcccaaacga cgctgcgttc
     9481 cgttatcggg cgtatggagt tggacaaaac gtttgaagaa cgcgacgaaa tcaacagtac
     9541 cgtcgtctcc gccctcgatg aagccgccgg ggcttggggt gtgaaagtcc tccgttacga
     9601 aatcaaggat ttggttccgc cgcaagaaat ccttcgcgca atgcaggcac aaattaccgc
     9661 cgaacgcgaa aaacgcgccc gtattgccga atccgaaggc cgtaaaatcg aacaaatcaa
     9721 ccttgccagt ggtcagcgtg aagccgaaat ccaacaatcc gaaggcgagg ctcaggctgc
     9781 ggtcaatgcg tccaatgccg agaaaatcgc ccgcatcaac cgcgccaaag gcgaagcgga
     9841 atccctgcgc cttgttgccg aagccaatgc cgaagccatc cgtcaaattg ccgccgccct
     9901 tcaaacccaa ggcggggcgg atgcggtcaa tctgaagatt gcggaacaat acgtagccgc
     9961 gttcaacaat cttgccaaag aaagcaatac gctgattatg cccgccaatg ttgccgacat
    10021 cggcagcctg atttctgccg gcatgaaaat tatcgacagc agcaaaaccg ccaaataagc
    10081 cgtgatgaaa ataccgtctg aagctctgtt ttcgggtttc agacggtatt tttatttaaa
    10141 cccgaacagg gttgtattcc gcttcaaata tgtttcggaa ccgtctacct gccccggtaa
    10201 aaactgccgg caaaatattt ctgcgcgccc tcgcgaagca agaccaaggt tacggcggcc
    10261 aaaggcaatc cggccaacat tccgacaaag cccatcagct ctccgaacgc catcagcgaa
    10321 aagataaccc aaaacggcga caggccgata cggtctccta caattttcgg cgtaatgaaa
    10381 aaactttcga gaaactgacc gacggcaaaa accgcccaaa cagccaagat tccgttccac
    10441 gaaccgaact ggagcaaggc tgcaacagtg gcaagcagca atcccgtaaa cgcacccaaa
    10501 taggggacaa acaccaaaat accggcaacc ataccgatgg caaatcccga atccagtccg
    10561 actagcatca atcccaaacc gtaaaccaag cccataatca gcatcaccag aagctgaccg
    10621 cgcaaaaatt cgcccaatac ctcgttcaaa ttacccgtaa tgcgcgtata agcaccggca
    10681 aaacgcctcg gaaccagttt ggcgatgccg cacgaccacc gctgccaatc cagcaggaaa
    10741 tagtaaagca gcaagggcgg cagcagcagg ttgccgatac tgctgacaat attgccgccc
    10801 tgtttcatca aaacgggaaa ccacgcctta agcgcgttgc tcaactcgcc cgtatgcgcc
    10861 tgaaaccacg caataataga tgcctgatcg atttccacat atccgccgat tgtatttttc
    10921 aaccacggca gcagcgtgtt ctgtataaaa ccgattaatt ggggcaggcg agatgccaaa
    10981 ttattgaact gcccgaccag catagggaca ataatcaaca ataatgccaa caacaaaatc
    11041 aaggaaaaca ccatcacaga catcgaagcg gatgcacggt tcaaaccctt tttctgcaac
    11101 cattcgacca aagggtccaa cacatacgcc agcaccgccg caaccgcaaa cggagtcaaa
    11161 gtatcgccga gcgcgtaaac cagccagacc aaggcggcaa acgccgcgcc ggcacccatc
    11221 cacggcttga tgccccgtcc ttttctccga tacataaaat ccacccttcc gaaaataata
    11281 ttgcgcgcca gtatagcaga accgccgcgc cgtcaaaaaa gcctccgcct tcccgatagc
    11341 ctgttatcgt ggtttaggct aaaatactgc acatccgatg ccctctgaaa ggcaaacccg
    11401 ccttcagacg gcataccgta cacgataagg cagcacatca tgaccgaaca aaaaaacaaa
    11461 gcatccctca tcgaatttcc ctgcgccttc ccattgaaag tgatgggcgc ggtgcatccc
    11521 gagttcgagc aggcggtatt ggacaccgtc cgccttcacg cccccgacac gcaggcgcac
    11581 cacatcacca cgcgtccgag cagcaaaggc aactataccg gtgccaccgt tcaggtaaag
    11641 gttgaaaacc aagaacaatt ggacaacatc taccgcgcgc tgacttcgca cgaactggtc
    11701 aaagtggtac tttgagatga aaatcataca caaaggtttg gtcgaatatc tgccgacttt
    11761 tgaagcgatg aaaaccttta atgccggacg gaatgcagac accgaagacg aattgtgggt
    11821 cgtcgaacac ccgcccgtct ttacgcaagg actggcggga aaacccgagc acctgctgat
    11881 tagggacgac atccccgtcg tccaaatcga ccggggcggg cagattacct atcacgggcc
    11941 cgggcaattg gtcgtttata cgatgattga tttcaaacgg cgcaaaacca gcgtccgcaa
    12001 catcgtttcc gcgcttgaaa acagcatcat cgccacattg gcggaatacg gcatcgaagc
    12061 ggcggcagat cccaaacgcc ccggcattta cgtcggcgaa cgcaaaatcg cctcactggg
    12121 gctgcgtatc aaaaacggtt ccgtctatca cggactcgca ctcaacgtca atatggattt
    12181 aagcccgttt acccaaatca acccctgcgg ctacgccggt atggaaatga cgcaaatcgc
    12241 ggattttgtc caaccctgcc ccgcgccgga cgaagtcgcc tccaaactca ccgcacacct
    12301 tgaaacacaa ctcacaccga aagcagacaa caatgagtga aatcaaaacc gacgacccca
    12361 aacgcggcat caaactcaga ggcgcggaca aaaccgcccg catccccatc aaagtcgtcc
    12421 cccttcagga aaaactgaaa aagcccgaat ggatacgcgc caaactccca tcgcgcaaat
    12481 tctttgaaat caaagacatt ttgcgcgaac aaaagatgca caccgtttgc gaggaagcct
    12541 cctgccccaa catcggcgaa tgcttcagca aaggcacggc gaccttcatg attatgggcg
    12601 acatctgcac ccgccgctgc ccgttctgcg acgtgggaca cggccgtccg aatatgctcg
    12661 accccgacga gccgaaaaac ctcgcagaat ccgtcaaagc catgaacctg cgttacgtcg
    12721 tcatcacctc ggttgaccgc gacgacctgc gcgacggcgg cgcccaacac ttcgccgact
    12781 gcatcaaagc catccgcgaa accagcccga acaccaaaat cgaaatcctc gtccccgact
    12841 tccgaggccg cttggacatc gcactgaaaa tccttgccga aaccccgccc gacgtgatga
    12901 accacaactt ggaaacccat ccgagcctgt acagaaaagc ccgtcccggt gccaattatc
    12961 aacattcttt agacttatta aaacgttata aagaaatgat gccgcatatc ccgaccaaat
    13021 ccggcattat ggtaggcttg ggcgaaacag acgaagacgt gcgtgaaatt atgcgcgata
    13081 tgcgggcgca caatatcgag atgattacca tcggacagta cctccagcct tcagacggac
    13141 acttgcctgt cttgcgctac gttacacccg aacaattcaa aatatttgaa aaagaagcat
    13201 acgaactggg ctttaccaat gccgccatcg gcgcgatggt acgttcaagt taccatgccg
    13261 acgaacaggc ggcggaagcc ttacgcgaaa gccacggcgg ttgcgggcat cattaaactc
    13321 ggaacaccgt tccgaagtaa aaaataattt cctacgctgc tgtctgccgc cttgtttgat
    13381 gtaaacaaca tcaatttgaa ttgtccatgc cgtctgaaag tccttcagac ggcataattt
    13441 attcccaaaa tccgataatc tcaaaagaat ctgtcggtta atgagaaaga ttcattataa
    13501 attttgataa aaaaccgtgc tctatttgtt ttttgctatg gttaacaagt gtaaatgttc
    13561 tttattgatg agatggattc ttagttttag cagcaaaaaa aaccgataag aaacagcata
    13621 ctgatttgga aggcatcggg gaatacgccc tatttatttt gataattgtt tgtattttaa
    13681 atagaaaatt tgtcatgccg acagcttttg catacaatgc acgcatcccg aacagcgtta
    13741 cagaacgccg tttaaccgca cgaaacccaa attaagaaaa gagagatatt atgcaaggca
    13801 atcaagctgt tgttgattac atgaacgaat tgctgtccgg cgagctggcg gcacgcgacc
    13861 aatacttcat ccactcccgc ctctactccg aatggggcta caccaaactt tttgaacgtc
    13921 tcaatcacga gatggaagaa gaaaccacac acgccgaaga cttcatccga cgcatcctga
    13981 tgctgggcgg tacgccgaaa atggcacgcg ccgaactgaa tatcggcacg gacgtggttt
    14041 cctgcctcaa agccgacctg caaaccgaat acgaagtgcg cgacgctttg aaaaaaggca
    14101 tcaaactgtg cgaagaggct caagactatg ttacgcgcga cctgatggtt gcccaactga
    14161 aagacaccga agaagaccac gcacactggt tggaacagca gctgcgcctg atcgagctga
    14221 ttggcgaagg caactactac caaagccaac tgtaatccgt attaggaata aaaggagtcc
    14281 gtatgaaagg cgaccgtttg gttatccgcg agctgaacaa aaacttaggc ttgctgctgg
    14341 taaccattaa ccaatatttc cttcacgccc gtattttgaa aaactggggc tttgaagaac
    14401 tgggcgaaca tttcttcaaa caatcaatcg tagaaatgaa agctgccgac gatttgattg
    14461 aacgcatcct cttccttgag ggattgccca atttgcaaga gctggccaag ctgttgattg
    14521 gtgaatctac cgaagaaatc atcgcctgcg atttgaccaa agaacaggaa aaacacgaag
    14581 ccctgcttgc cgccatcgcc acagcagaag cgcaacagga ttatgtcagc cgtgatttgt
    14641 tggaaaaaca aaaagatact aacgaagaac acatcgactg gctcgaaacc cagcaggaac
    14701 tgatcggcaa aatcggtttg ccgaactacc tgcaaacagc ggcgcaagag gactaaaaca
    14761 caaaccactg ccaaatacag cagtcccctg catcatcaga cggcatatcc gtataaacgg
    14821 gtatgccgtt tttctttgcc ggtttacgaa taaaatctaa attgatttga atcaatactc
    14881 gataacggtt tttgtttgat aataccgata tgaaatctag attgtttgtt tgctgtttcc
    14941 caatccgtct tgattttacc tctttcctct tgatgtgtgt gtgtgtttgg gtgtggctgc
    15001 cgccacccct tttttttggc ttttatgtga agtaaaatcc gtaacagcaa atacttaact
    15061 tttaaccatt tatccggaaa caaatgatgg gcaacaaatt gactctgcct gccgaattgc
    15121 ctgacgaaca ggatttgcgg gcggtattgg catacaatat gcggcttttc cgcgtgaaca
    15181 agggttggtc gcaagaggaa ttggcgcggc aatgcggttt ggacaggact tatgtgtcgg
    15241 cagtcgagcg caaacgctgg aacattgccc tgtcgaatat cgaaaaaatg gcggcggctt
    15301 tgggcgtggc ggcgtatcag ttgctgctgc cgccgcagga acggttgaac ctgatgacca
    15361 attccgccga tacccgacaa gtgccgtctg aaagcggtat ttgaccgccg aaccatccgc
    15421 ctcccttttt cccgccaatc gaaagaataa aaatgcctga aaacctgtct tcagcactgg
    15481 aaaccttcaa acagcagcgc aatgccgccg aagcgcatta tttgaaagcc aaccgcgtgt
    15541 cggtattttt cagagaatac acggcggcag tcgaaacctt gctggcggca ttgtgggcag
    15601 aacattttca aaacagcgcg ttatgcctga tggcggtagg cggcttcggg cgcggcgaac
    15661 cgtatccctg ttcggatgtg gatttggcgg ttgtctcccc tgccccgctt tcagacggca
    15721 ttcaggaaca gattgcccga tttatccaaa ctctgtggga ttgcaaacta atgccgtctg
    15781 taaaaagcgg cagcgttgac gaactgtgcg aaagcgtgcg cgacgatatt acgggggaca
    15841 cagcgttttt agaggctagg tttttgtttg gcaaccgcca aacagcagac gaattggcgg
    15901 aaaaaatgaa cgtgcaacgc aatgtagcgg catttatcga agcaaaattg gtggagatgg
    15961 aacaccgcca cgccaaatcg caaggttcgg gggcggtgtt ggagccgaat atcaaaagct
    16021 gtccgggcgg tctgcgcgac atccacaccc tgctttggat agcgaaggcg caaggcttgg
    16081 cggccaacct gcccgacctg ctcaaacagc ggattttgac acgtgccgaa gccggtatgc
    16141 tttcgcacgg ctaccgccgc ctcgcccaca tccgcatccg gctgcattta aacgccaagc
    16201 gcgccgaaga ccgcctgctt ttcgatttgc agccgcaagt tgccgaaagt atgggttatc
    16261 aagatgaaaa ccgccgccgt caaagcgaag aactgatgcg cgtgttttac cgtgcggtca
    16321 aaaccgtcaa acaactgggc ggcatcctca cgcccatgct gcgaagccgc gtttcctcca
    16381 cgccggtgcg cgttaccctg cggattgacg acgactacat ccaagtcaac aaccaaatcg
    16441 ccgcgcggca caccgatatt tttttcagac ggcccgaaca cattttcaaa atcgtcgaaa
    16501 tcatgcagca gcgcaacgac attactgcgc tcgaaccgca aaccctgcgc gcatggtggg
    16561 gggcgacgcg caaaatcaac cgcagcttct accaaaattc tgaaaaccgt cgccgcttcg
    16621 ccggtttttt ccgcagcggc aacggactga cccaaaccct gcgctttctc aacctctacg
    16681 gcgtgttggg ccgctatctg cccgcgtggg aaaaaatcgt cggcctgctt caacacgact
    16741 tgttccacat ctatcccgtg gacgaccaca tccttgccgt cgtccgcaac gtccgccgcc
    16801 ttgccctaga tatgcacagc catgagctgc cctacgcctc cgcgctgatg cagtcctttg
    16861 aaaaacaaga catcctctac cttgccgcct tcttccacga catcgccaaa ggacgcggcg
    16921 gcgaccatgc cgtacaaggc atcgcagacg cgcgccaatt tgccgccgac cacttcctga
    16981 ccgaagaaga aagcgacctg ctcgcctggc tggttgaaaa ccacctcctt atgtctgccg
    17041 tcgcccaaaa agaagacatc caagaccccg gcgtactcga tgccttctgc aaacgcgtcc
    17101 aaacccacga acgcctcagc gcgctctacc tcctgaccat ttccgacata cgcggcacca
    17161 atcccaagct gtggaacgcc tggcgcgcca gcctgctgga aagcctgttc cacgccgccg
    17221 gacgctgcct cgcaggcaac gacggcaacc cgcacgccct cttcggccgc cgccggcagg
    17281 aagccgccga cttacttacc cgcgccgccg tccccgaaaa acagcagaaa aaactatgga
    17341 acgcgctcgg ttccgcctat ttcgcccgcc atcagtcccg cgaaatcctg tggcacgccg
    17401 ccaacctcgt acacgacttt gaacccccca tcgtccgcag ccgcatccta ccccaaagcg
    17461 acagctttca agtgatggtc tttatgccca acggcccgcg cctgttcgcc cgcctctgcc
    17521 gcatcttcag ccgccacggc ttcgatatcc tcgccgcccg cgcctttatc accgaacacg
    17581 actacatcct cgacaccttc atcgtgcaaa tcccctcgca gcacgccccc gaagactacc
    17641 ccgacatcca aagcgcgctc gaagccgaac tcaacagctt tatccacgga cacaccgttg
    17701 ccgaaaccca aagctgcaac cgccgcatca gccgccgcag ccgctatatg cctattgcgc
    17761 caagcatcac catcaccccc gaagaagact atccggaccg gtattccgtc gaaatcaccg
    17821 ccgtcaaccg ccccttcctg ctcgccgaca tggcggaagt cttcttcgcc cacaacgtca
    17881 gcctgcgcta cgccaaaatc tccacgttgg acgaacgcgt cgaagacagc tttaccgtct
    17941 tcagccccga cttgaaaaac ccaaaaatcc agtcctcatt gaaacaggct ttgctggaac
    18001 agttggcata agcaccctgc cggttttcaa acggcatccc gccgccacgc cgtatgccgt
    18061 ctgaaggcgg gggatattgc aaacaatata aactgtcaag ccgccggcaa cgtgctataa
    18121 tgccgtgcat cttgcacaaa ccgaaagacc gagcattatg cgtatcgtag aaaaagccta
    18181 tactttcgac gatgttttgt tggttcccgc acattcgacc gtgctgccgc gagacgttaa
    18241 acttcaaacc aagcttaccc gcgaaatcac actcaacctc cccctgcttt ccgccgcgat
    18301 ggataccgtt accgaggcgc gcctcgccat ttcgatggca caggaaggcg gcatcggcat
    18361 catccataaa aacatgccgc ccgaaatgca ggcgcgcgcc gtttccaaag taaaacgcca
    18421 cgaaagcggc gtggtcaaag accccgtaac cgttgcgccg acaacgctca tccgcgaagt
    18481 cttggaaatg cgcgcgcagc gcaaacgcaa aatgtccggc ctgcccgtcg ttgaaaacgg
    18541 caaagtcgtc ggcatcgtaa ccaaccgcga cctgcgtttt gaaaaccgcg tcgatttgcc
    18601 cgtttccgcc attatgaccc cgcgcgaacg tctggttacc gtccccgaag gcacaagcat
    18661 agacgaagcg cgcgaactga tgcacacgta caaagtcgaa cgcgttttgg tcctgaacga
    18721 aaaagacgag cttaaaggct tgattaccgt taaagatatt ttaaaaacca ccgagtttcc
    18781 caatgccaac aaagactccg aaggccgtct gcgcgtcggt gcggcagtcg gcaccggcgg
    18841 cgacaccgac gagcgcgtca aagccttggt cgaagccggc gcggacgtga ttgtcgtcga
    18901 tactgcccac ggtcatagcc aaggcgtgat cgaccgcgtg cgttgggtca aagaaaccta
    18961 tccgcacatc caagtcatcg gcggcaacat cgccactgcc aaagccgctt tggatttggt
    19021 tgccgccggc gcggatgccg tcaaagtcgg tatcggtccg ggttcgattt gcaccacccg
    19081 tatcgtggca ggcgtgggtg tgccgcaact gaccgccatt cacaacgttg ccgaagccct
    19141 caaaggcacg ggcgttccgc tgattgccga cggcggcatc cgcttctccg gcgacatcgc
    19201 caaagccctc gccgcaggcg cgtacagcgt catgctcggc ggtatgtttg ccggcacgga
    19261 agaagccccg ggcgaaatcg aactctacca aggccgctcg tacaaatcct atcgcggtat
    19321 gggttccttg ggcgcgatga gccaaggttc tgccgaccgc tacttccaag acaaaaccga
    19381 cagcaccgac aaatacgtcc ccgaaggcat cgaaggccgc gttccctaca aaggcccgat
    19441 tgtgaacatc atccaccaac tgaccggcgg tttgcgttcc agtatgggtt atttgggttg
    19501 cgccaatatt gccgaaatgc acgaaaaagc cgaatttgtg gaaatcactt ccgcaggtat
    19561 gagcgaatcg cacgttcacg acgttcaaat taccaaagaa gcgccgaatt accaccgctg
    19621 atttgaacag ccttttcaag gcaaaatgcc gtctgaaacc taatttccgt gtttcagacg
    19681 gcattttttg tccgttcaag gcaagccgca cgggctgatt gcgcggacag aaaaagccat
    19741 tctgacggac acgccgaaag aatgaaaatg ccgtctgaac gccgtttgca ccccgtctgc
    19801 cgaataccgt cggcctttaa aacggcggtg gaaataaacg caatctttca atacgggcgt
    19861 ttcgattggt cggaaatgat gcgccggccg ccatatcaaa aaatcgggcg gccgcttcgg
    19921 gaaagtatgc cccgccctcc ccaagccgcc ccgtcagcat tatgatttgc ctttccgttt
    19981 tttgacctta atcggaaccg gtttctttgc ctttttcccc gattcggcaa cggcaggcac
    20041 gccccttccc cttactttgc cgcatcgggc tgtttttttc tcggcggtgg ttttcgattt
    20101 ccctttcccc gccgcccctg ccggtttggc agatgcggat aatttgacct tccgccgcct
    20161 gccgctttct ccggcaatta ggacaaagtc gatttttcca tcatccaaat cggcacgcgc
    20221 gacccggacg gcaaccctgt cccccatatt gaaacggatg ccgctgcgtt cgccttcgat
    20281 tgccatgatt tcggggcgga agttgaaata atcttcgccc aaatcgctga tatgtaccag
    20341 accgtcgata tggatatcgt ccaaagtgac aaatattcca aaatttgcca ccccggagat
    20401 tttgccttca aatatttcac cgaccttatc gcgcatataa taagttttca gccagttttc
    20461 cacatcgcgg ccagcatcgt cggcacggcg ttcgcaaaac gaagtatgca cgcccaaagc
    20521 ctgccagctt ttgtttggcg tgtaggtttt ccggttcaat acggctttga tggcacggtg
    20581 gacggtcagg tcgggatagc ggcggatggg cgaggtaaag tgggcgtatg cttcataagc
    20641 caaaccgaaa tgcccttcgc aatgcggttc gtaaaccgcc tgctgcatgg agcgcaacat
    20701 catgacttgc agcaattcgg catccggcct gcctttgaat tgttcggcaa gcgcggcata
    20761 gtctttcggc gacgggttgt cgccgccgcc aagttgaagc cccaacagac cgagctgctc
    20821 gcgcagggtg gcgagttttt cgggcgtggg gcccaaatgg ttgcggaaca aagccgtatg
    20881 tttgtttttc aacagaaaat ccgccgcgca aacattcgcc gccagcatac attcttcaat
    20941 cagcttgtgg gcatcgttgc ggacgacggg cacgattttt tcgattttgc cgttgtcgtc
    21001 gaaaatcatc tgggtttcga cgctttcaaa ctccaccgcc ccgcgcgcca gacgtttttt
    21061 ctgcaaaatt ttaaacagct tgtaaagcgt gtcgatttgg gctttgtgcg gattcccgat
    21121 gccgtctgaa agccatttcc aaacttggtt gtaggtcagg cgggcatgag agcgcatcac
    21181 ggcgggatag aagcggtatt ctttgatatt gcccgcatag gtaacgacca tatcgcacac
    21241 catacacaaa cgctcgacat cgggattgag cgagcagatg ccgttggaca ggttttccgg
    21301 cagcatcgga atcatacggc gcgggaaata cacgctggta ctgcgttctt gagcatctgc
    21361 atcaatcgca tcgtcagggc ggacataatg gctgacatcc gcaatcgcca cgaccagacg
    21421 gtaattgcgt ccgacttttt cggcaaacac cgcgtcgtcg aaatcgcgcg ccgtttcgcc
    21481 gtctatcgtt accaaaggaa ggtcgcacaa atcgacgcgg cctttcaaat cgcttttgcg
    21541 tacatggtcg ggaatttttt tcgcggattt ggcacacgct tcactgaatc ggtgcggcaa
    21601 atgatgcttg cgcacggcaa tttcgatttc catcccgctg tcggcataat cgcccaaaac
    21661 ttcaatgatt tttgccactg caggccggtt ttgctcggga taaacctcaa ttttgccgac
    21721 gataacctga ccggattcgg gtttgaaacg cgccacgccg tccggttcca acacgatgct
    21781 ttggttcaga cgcttgtctt ccggctccaa aatcgccacg cccctatcca tatagaaacg
    21841 gccgacaact ttgctttgcg cgcgttcgac aatatccaga accgtccctt cgcggcggcc
    21901 cctgcggtcg atacccgcag gacggacggt aacggtgtcg ccgtgcatga caccacgcat
    21961 ctggcgttcg tataaaacga aatccccttc gtccatcggc atgagcggca cggcgaagcc
    22021 gaaaccgtcc ttatgcgcct cgacgcggca tttgaccaaa tccagcttgt ccgccgcgca
    22081 aactgcgcct cggcggttga ttaaaacctg accgtcccgc gccatcgcct tcagacggcg
    22141 ttcaaaaaag acatactcgt cttccgtaat cgacagctcg cgtgcaagcg attcgatttt
    22201 tgaaggcaca cctttgcgct ccaacaattc gattatccat tcccgactgg gcaaaggatg
    22261 ttcataacgc tgtttttcac gacttaaaaa cgggtctttt tcccgtaaat ttaaagattt
    22321 aatattttta ttcattttga ctgttgacat tctctttatg aactatataa tagcgacttc
    22381 tttgcggcag gacactgtac cgcaaaacag caaataaagc aaagcccggg tggcggaatt
    22441 ggtagacgcg ccagcttcag gtgctggtat cctcacgggt gtggaagttc gagtcttctc
    22501 ccgggcacca aataacaaat tatcccgatt aggataattt ccgccattag agaggtggat
    22561 gagtggttta agtcgcacgc ctggaaagcg tgtatacgtg aatagcgtat cgagggttcg
    22621 aatcccttcc tctctgccaa gtacaaatcc caaactttta cagtttggga ttttttgtta
    22681 cttacgtatt gattttattg cgtaaaatca gccacaacct attaccctac gttaccattg
    22741 attactatcc gttaccgttt ttttatggta tgttttgcgg tatgttttat cctcttccta
    22801 agcctgtaag gactcaaaag gcgtatccct tcaaacgata ttgttcgaca tgctgccggg
    22861 aaaccgaacc cgataccgaa acgcgacggg gcgatatgcc gctggaaaaa tggcggcagt
    22921 ttcttgattg gataggaaaa acgccgtctg aaacagcttc aagaagccgc aaagcccgtc
    22981 cgaataaaaa ataccgtctg aaatttttca gacggcatcg cgtatcattt aaacattaat
    23041 ccagcttttt aaaatgacgg cggcgttcca attcgctcag gtaacgcttg cgcaggcgga
    23101 tggattgcgg cgtgatttcc accagctcgt catcgtcgat aaactcgacc gcgccttcca
    23161 gcgtcagttt gatcggcgtg gtcaggcgca ccgcttcgtc ggtaccgctg gcacggatat
    23221 tggtgagttt tttgcctttg agcgggttga ccaccaaatc gttgtcgcgg ctgtggatgc
    23281 cgataatcat accttcgtag attttgtcgt tgggcgatac gaacatacgg ccgcggtctt
    23341 caagattcca caaggcgtaa gcaaccgcct cgccctgctc ttgggacacc agtacgccgt
    23401 tgtggcggcc gggcatatcg ggtttgacgg gcgcgtagtc gtcgaacacg tggctcatca
    23461 gcccgacgcc gcgcgtcagg gtcatgaatt cgccttggaa accgatcaag ccgcgcgctg
    23521 gaatatggta ttcgaggcgg gtgcgtccgt tgccgtcgct ttccatattg gtcagttcgc
    23581 cacggcggcg gccgagttct tccattaccg cgccttggtt gtcgtcgggt acgtccacag
    23641 tcaggttttc ataaggttcg catttttgac cgtcgatgtc tcggtacacg acgcgcggct
    23701 tgccgacggc gagttcgtag ccttcgcggc gcatattttc cagcaaaatc gtcaggtgca
    23761 gttcgccgcg cccggatacg cggaacacgt cggcatcggc ggtgtcttcc acgcgcaggg
    23821 caacgttggt cagcaattct ttttgcaggc ggtcgcggat ttggcggctg gtcacgaatt
    23881 tgccttctgt acctgcgagc gggctggtgt ttaccataaa gtccatcgtc agcgtcggtt
    23941 cgtccactct caacatcggc aggcctttgg ggttgtcttt gtcggtgatg gttacgccga
    24001 tgccgatgtc ttcgataccg gaaataatca cgatgtcgcc ggcttcggct tcttcaagcg
    24061 gcacgcgttc caagcctttg aaacccaaaa gctggttgat gcggccttgg gcgatttgct
    24121 gctcgtggtt catcacggca acggtttgac cgggtttgat gcgtccgttc aagatacgac
    24181 cgataccgag gcggccggtg tagttgtcgt agtcgagttg ggaaatttgc agttgcagcg
    24241 gctcgtccgc gctgccgctc ggtgcaggcg tgtattttag gatggtgtcg aacagcgggc
    24301 gcatatcgct gctctcgtcg gtttcttcca gcttggcaaa gccgctcaaa cctgaagcgt
    24361 aaacaatcgg gaaatccaac tgctcgtcgg tcgcacccaa gttgtcgaac aactcgaaag
    24421 tctggtcgat aacccagctc ggacgggcgg acggtttgtc gattttgttg atgacgacaa
    24481 tcggtttcag ccccaaagcc aaggcttttt tggtcacgaa acgggtttgc ggcatcggac
    24541 cttcctgtgc gtccaccaac aagacgacgc aatccaccat ccccaaaacg cgctccactt
    24601 cgccgccgaa gtcggcgtgt cccggcgtgt cgacgatatt gatgtggcag ccttcgtaat
    24661 cgatggcggt gtttttggcg aggatggtga tgccgcgttc tttttcaagg tcgttgctgt
    24721 ccatcacgcg ctcgtcaacc tgctggttgg cgcggaatgt gccggattgg cgcagcagtt
    24781 ggtcgaccaa tgtggttttg ccgtggtcga cgtgtgcgat gatggcgatg ttgcggattt
    24841 gtttcataat taatgttttt caaaaactgt aagagataac tgcgtattat accacgattt
    24901 accggacagt atttggaaaa tgaagatgcc gtctgaagcc cttgcctttc agacggcatc
    24961 ttcttacata ctttcagtta tccagttgcg cagccacctt gccgccgggg cgatttcccc
    25021 tgccgacagc ccagccgcaa tgcctgccga ttcctttata acatccgccg ccgcgccgtg
    25081 cagccacgcg cccgcgcagg cggcttcaaa aaccggcacg ccctgtgcga gcagactgcc
    25141 gatgatgccg cccaatacgt cgccactgcc cgccgttgcc aatcccgcat tgccgctttc
    25201 gttgacatag atttccgtat cggacgcggc aaccaatgtt ttgtgcccct ttaaaaccac
    25261 ggttgcaccg agaattgccc ctatcttcct tactgccgcc gtccgatccg cctgaacctg
    25321 tgcaactgtc gttccaagca ggcgcgcggc ttcggcgggg tgcggcgtta aaatcaggtt
    25381 tttacacccg cgcgccagat ttcgggtttc ggcatcggtt gataatatgt tcagcgcgtc
    25441 cgcatccaaa acgacggctt cgtctcgatt ccgcgccagc actgccgcca ccgtttccac
    25501 tgccgccgca tctgtaccca agccgcaacc cgccgtccaa gcgtttatgc cttgacgctt
    25561 ggtcaaaccg tctgccgtgt ccaacataat ctcgggaaaa ccggcaatga cggcaaaagg
    25621 cagcgtatcc tgattgaaac ctgcccgcac cttaccgcag ccgagataca ttgccgccga
    25681 tgccgccaat acgggcgcgc cgctcatccc tgccgatccg ccgactacgg cgagcgtgcc
    25741 gaaagtccct ttgtgcgaat cttccgcacg ggctttgaaa acatggggaa aacgcaatgc
    25801 cgtctgaagc atccggcggc ggctttcgcc tgaaagatgg aaaacgggaa acatttcccc
    25861 tccaatcggt taaaattaag gctcgaaaac aaaccgattc taccgccgga aaccgattat
    25921 gaaaaacaaa accaaagtct gggacttccc cacccgcctt ttccactggc tgcttgccgc
    25981 atccctgccc tttatgtggt atagcgcaaa agccggcggc gatatgctgc aatggcacac
    26041 gcgcgtcggg ctgctcgtcc ttttcctgct cgtattccgc ctctgctggg gcatttgggg
    26101 cagcgatacc gcccgtttct cccgtttcgt ccgaggttgg gcaggtatac gcggctatct
    26161 gaaaaacggc attcccgaac atatccagcc cggacacaac cccttgggcg cactgatggt
    26221 cgttgcgctt ttggccgccg tctcatttca agtcggcacg gggctttttg ccgccgatga
    26281 aaacaccttc agcaccaacg gctacctcaa ccatttggtt tccgaacata cgggcagcct
    26341 tatacggaaa atccacctca actttttcaa gctgctcgcc gttttttccg cagtccacat
    26401 cgccgccgtc gccgcatacc gcatattcaa aaagaaaaac ctcgtccgcc cgatgataac
    26461 cggcttcaaa tacatcgaag gcaaaacctc aatccgcttt gccggcaaag ccgcgcttgc
    26521 cgccgcatta tcggttgccg cgcttgccgc agccgccatc ctgctcctgt cctgaaaccc
    26581 gacatcatgc cgtctgaagc ccttttctgc ctttctgacg gcattttccc caccgcacaa
    26641 acggtctgaa tgcccgaaac ggcttcaggc cgtttttccg tccaaaaccg ttatatagcg
    26701 gattaacaaa aatcaggaca aggcggcggg ccgcaggcag tacaaatggt a
//
LOCUS       DAXDXW010000022        24889 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXDXW010000022 DAXDXW010000000
VERSION     DAXDXW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24889
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.022"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(59..253)
                     /locus_tag="ACFB64_000810"
     CDS             complement(59..253)
                     /locus_tag="ACFB64_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC1209203.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMNK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(243..2324)
                     /locus_tag="ACFB64_000811"
     CDS             complement(243..2324)
                     /locus_tag="ACFB64_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC1209204.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMTVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRHIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLNFPVDAATLLHTAKEVGENTILSRTGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVALVPAVGVLLVTCYAGLQKLFHNDPRASFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVTVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEEQSEA"
     gene            2848..3228
                     /locus_tag="ACFB64_000812"
     CDS             2848..3228
                     /locus_tag="ACFB64_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC1209205.1"
                     /translation="MNIRFFALTVSVLSLAACAVPEAYDGGGRGYMPPVQNQAGPDDF
                     RAFSCENGLSVRVRNLDGGKIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3364..3852)
                     /locus_tag="ACFB64_000813"
                     /pseudo
     CDS             complement(3364..3852)
                     /locus_tag="ACFB64_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
     gene            4184..5572
                     /locus_tag="ACFB64_000814"
     CDS             4184..5572
                     /locus_tag="ACFB64_000814"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFC1209206.1"
                     /translation="MNEYSQLIKHPDISLSPISDGIGVGNPATGEISAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIVKP
                     ASLTPLSAYALAVLAYEAGVPQDLLPVVSGRASEISHEFATNPTVRKISFTGSTEVGA
                     KIFAGSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKFRNSGQTCVCTNRVYV
                     QSGIYDVFCRKLSEKAAALKLGDGLDEGVNQGPLIEEKAVEKVEQHIADALSKGAVCL
                     TGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFETEAEVIEAANNTEYG
                     LAAYLFTSDTARQWRVGEVLEYGMVGINTGLISNEAAPFGGVKRSGLGREGSKYGADE
                     YLELKYLCIDAG"
     gene            5683..6468
                     /locus_tag="ACFB64_000815"
     CDS             5683..6468
                     /locus_tag="ACFB64_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC1209207.1"
                     /translation="MMENGKTFPRWRFALKSAGWHLLISLLVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIIP"
     gene            6836..8392
                     /locus_tag="ACFB64_000816"
     CDS             6836..8392
                     /locus_tag="ACFB64_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC1209208.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARITGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFAVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMVVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESV
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDGTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLEAIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDTDFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8409..9155
                     /gene="surE"
                     /locus_tag="ACFB64_000817"
     CDS             8409..9155
                     /gene="surE"
                     /locus_tag="ACFB64_000817"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC1209209.1"
                     /translation="MNVLISNDDGYLAEGIAILARVASEFANVRVVAPERDRSGVSNS
                     LTLDRPLQLKQAQNGFCYVNGTPTDCIHVGQFALPDFKPDVVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGMPAVAFSLNDASGRYWATAEKALWTLLAHFFKKPPSAPVLWNVN
                     IPAVAPEDVRGIKITRLGRRHHEQNIVPSRNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHTD"
     gene            9257..10270
                     /locus_tag="ACFB64_000818"
     CDS             9257..10270
                     /locus_tag="ACFB64_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC1209210.1"
                     /translation="MLKQTTLLAACTAVAALLGGCATQQPAPVIAGNSGMQTVSSAPV
                     YNPYGATPYNAAPAANDAPYVPPVQTAPVYSPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKTAAVE
                     SRPAVPAAAQTPVKPAAQPPVQSAPQPAAPAAENKAVPAPAPAPQSPAASPSGTRSVG
                     GIVWQRPTQGKVVADFGGGNKGVDIAGNAGQPVLAAADGKVVYAGSGLREYGNLVIIQ
                     HNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSY
                     IAF"
     gene            complement(10672..11154)
                     /locus_tag="ACFB64_000819"
     CDS             complement(10672..11154)
                     /locus_tag="ACFB64_000819"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC1209211.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRREISLQASGDVSCGC
                     "
     gene            complement(11307..11468)
                     /locus_tag="ACFB64_000820"
     CDS             complement(11307..11468)
                     /locus_tag="ACFB64_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209212.1"
                     /translation="MSEQPEKHHASPIEDERKKPVYRMGRAVAGFMLAVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(11542..12003)
                     /gene="recX"
                     /locus_tag="ACFB64_000821"
     CDS             complement(11542..12003)
                     /gene="recX"
                     /locus_tag="ACFB64_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC1209213.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEKTSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAANLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDSC"
     gene            complement(12076..12786)
                     /locus_tag="ACFB64_000822"
     CDS             complement(12076..12786)
                     /locus_tag="ACFB64_000822"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC1209214.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLVIITNKNEILAAELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDAANMLMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDTTRPDRIIG
                     ALPEIYENLRPQKNKDEE"
     gene            13181..14400
                     /locus_tag="ACFB64_000823"
                     /pseudo
     CDS             13181..14400
                     /locus_tag="ACFB64_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
     gene            complement(14460..15266)
                     /locus_tag="ACFB64_000824"
     CDS             complement(14460..15266)
                     /locus_tag="ACFB64_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC1209215.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVTAEYQ
                     PTFRSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDTT
                     DGKGEVDIIPLRQGFWKASVEYKADFPDQSLCQKQANYTTLTFQIGHSHH"
     gene            15458..15667
                     /locus_tag="ACFB64_000825"
     CDS             15458..15667
                     /locus_tag="ACFB64_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC1209216.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVIELLARVLGKNPETTVV
                     VIDEVDTDNWDIGGKSVSERRKEGR"
     gene            15756..16970
                     /locus_tag="ACFB64_000826"
     CDS             15756..16970
                     /locus_tag="ACFB64_000826"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFC1209217.1"
                     /translation="MDKFPKSAKLDHVCYDIRGTVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQNKGLRDITVNDVYIGN
                     GVSELIAMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITSKTKAIVVINPNNPTGAVYSKEILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHCHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYHQ"
     gene            complement(17042..19621)
                     /gene="clpB"
                     /locus_tag="ACFB64_000827"
     CDS             complement(17042..19621)
                     /gene="clpB"
                     /locus_tag="ACFB64_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC1209218.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADSSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGEILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEIDG
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGADEVAEIVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQNEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIKVEADGDRL
                     KFA"
     gene            19881..20891
                     /gene="trpS"
                     /locus_tag="ACFB64_000828"
     CDS             19881..20891
                     /gene="trpS"
                     /locus_tag="ACFB64_000828"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC1209219.1"
                     /translation="MSKKRVLTGVTTTGTPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEQEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFQELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAEFTQMLADGLAWGEAK
                     KLSAAKINAELAELRERYNALTSNPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            20972..21517
                     /locus_tag="ACFB64_000829"
     CDS             20972..21517
                     /locus_tag="ACFB64_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209220.1"
                     /translation="MLKIPFAVLGGCLLLAACGKSENTAEQPQNAAQSAPKPVFKVKY
                     IDNTAIAGLALGQSSEGKTNDGKKQISYPIKGLPEQNAVRLTGKHPNDLEAVVGKCME
                     TDGKDAPSGWAENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            21834..22178
                     /locus_tag="ACFB64_000830"
     CDS             21834..22178
                     /locus_tag="ACFB64_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209221.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAAQAVESDVKDTAASAA
                     ESAASAVEEAKGQVKDAAADAKASAEEAVTEAKDAAAETKEAVSEAAKDTLNKAADAA
                     QEAADKMKDAAK"
     gene            complement(22388..23896)
                     /locus_tag="ACFB64_000831"
     CDS             complement(22388..23896)
                     /locus_tag="ACFB64_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC1209222.1"
                     /translation="MTTNPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSTSSQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPCGGKMLVIDIGGSSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMGGIIGGNEMLWYAVLSLRLAALFCRSRQDLGFP
                     KNMQLRTDTESCGFILRIDRKWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(24059..>24889)
                     /locus_tag="ACFB64_000832"
     CDS             complement(24059..>24889)
                     /locus_tag="ACFB64_000832"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC1209223.1"
                     /translation="NASSEKTIEFAGRSWTKTADSLPQKAHGLRYGLGVSREINAGGH
                     HFLYGGISRGVHYWDNKDFSEQSLRLSFGHQNRSVTRSFGTVPFVGQNLLGGSRYNFA
                     GGFNADFSRRLGERWRLTLNAGNMWKHYQEDRTAARYGSHMPLAGATLMYSAPKDRLL
                     YGGADWPHDMTKEAEQASVRKGLRVGAVKTFDGGLGLRANLRYTRRTFDAPGTIVYRF
                     PRKDHEYQANLSLRHDKISWKGFTPQLNFRYLKIDSNMKSFYTRKNTQIFMSVEKDFK
                     "
BASE COUNT         5691 a         6270 c         6905 g         6023 t
ORIGIN      
        1 ccttattata aaaataccgt ctgaagtggc gttcagacgg catttgtttc aagcggggct
       61 aacagcagcg tccgccgttt gcgccgcagc ggcgtttgcg gcaatagtct tgaaattgca
      121 gcttgttcat cacgggggcg ttgggattat gtttacgttg ttgtgcaacg tagttttcat
      181 aatcgggcac gcctgccatc aagtttgccg tcagcttgat ggttttccac caagacgcga
      241 gcttatgctt cactttgttc ctccggttgt ttgccgtcgc ggtacaccgc cgggatttct
      301 ttggcggtcg gccagccgac tttgcgtgct ttgagggcgg tacgcaaacc gtacgcggca
      361 acagtcacga caaccgacaa gaagaggatg gtcagaccgg cattaatctt gtcgttgaag
      421 atgatttgcg ccatttcgcc gatgtcttta gcgggcgcaa ggacttcgtt tttagccaat
      481 gcgtcgctgt atttgcctgc gtgggcaagg aagctggcgc gcgggtcgtt gtggaacagt
      541 ttttgcagac cggcgtagca ggttacaagc agtacgccga cggcaggaac gagtgccacc
      601 cagacataac ggtcgcgttt catcttaatc agcacaacgg cacacataat caaggctacg
      661 cctgccagca tttggttggc gatgccgaat aaaggccaga gcgagttgat gccgcccagc
      721 gggtcggtta cgccggtgta gaggaagtag ccccacaatg ccacggcgaa gaaggtcgca
      781 atcaggttgg cggggatgga gtcggtgttg ccgaaaggtt tgtagaagat gctgcccaag
      841 tcttgaatca taaaacgtgc gacgcgcgta ccggcatcga cggcggtcag gatgaacaag
      901 gcttcaaaca acagggcgaa gtgataccag aatgccatca tcgcctcgcc cggaatcaga
      961 cggctcataa tgtgcgccat accgactgcg agggtaggcg caccgcctgt gcgggacagg
     1021 atggtgtttt cgcccacttc cttagcggtg tgcaacaggg ttgcggcatc gacagggaag
     1081 ttcagcttgg tggtaattac ttcggcggcg gtgttggcat ccgtaccgat cagtgcggcg
     1141 gggctgttca tcgcgaaata cacgccggga tcgagcgatg cggcggcggc aagcgccata
     1201 atcgccacga aactttccat caacataccg ccgtaaccga tcatacggac gtgggtttcg
     1261 ttttccagca ttttcggcgt ggtgccggaa gaaatcagtg cgtggaagcc cgaaaccgca
     1321 ccgcaggcga tggtaatgaa caagaacggg aacaatgcgc ctgagaatac cgggcccgaa
     1381 ccgtcgataa agtgggtaac cgcaggcatt tgcaaagcgg gattgacgat gacgataccc
     1441 aaagccaagg ccgcaatcgt accgattttc aggaaggtgg agagatagtc gcgcggggtc
     1501 agcaagagcc agaccggcaa aaccgaagcc acgaaaccgt aaatcataat cgcccaagtg
     1561 agctggatgc cgtcaaggtc aaaccaatgc ccgatggagc ttttcgccac atcttcgccg
     1621 taaattaccg ccaacatcag caaaataaag ccgacgatgg aaatctcgcc gattttgccc
     1681 ggacggatat ggcgcgtgta aatacccata aacagcgcaa tcggcatcgt tgcggcaatg
     1741 gtgaacgtac cccaagggct gtgaaccaat gcttttacga caatcaacgc caaaaccgcc
     1801 ataatgatga ccataatcat caaaataccg atggaggcaa tcacgccggg gacagtgccg
     1861 agttcctgtt tcacaatatc gcccaaagac ttaccgtcgc ggcgcataga gacgaacaag
     1921 accgtcatat cctgtaccgc gccggcaaat accacgccga agataatcca caaagtaccg
     1981 ggtagataac ccatttgcgc cgccaaaacc ggaccgacca aagggcccgc gccggcaatc
     2041 gcggcaaagt ggtgtccgaa caatacgcct ttgtgcgtcg gaacgtagtc caagccgtcg
     2101 ttgtgccgct cggcgggcgt gagacggttc ggatcgagcc gcatcacgcg gttggcgatg
     2161 tagaggctgt aaaaacggta ggcgatacag taaacggata tggcggcggt aaccatccat
     2221 accgcgctga cctgttcgcc tcggctgagg gccagagtgg taaaggatgc taagccgacc
     2281 agtaccacta tgccccaaat gaggaaggtt ttgagtgatt tcatcgaata aatccttatc
     2341 tcacactgtc ggaatatgtc ggagcggaca ggcaacggtt ggctacccgt aatccgatgc
     2401 cgacacaaac cggagtttga atgattgacg aagtatgaaa cagtgcgctt gtcgggactt
     2461 gtgccttatg cccgcatcaa acagtgcagg aatgtacgcg cacaaacgtt aaacgcccga
     2521 ataggatttt aacgcaaatt agcacactga tatcggtttt tacttggaat ttggaaaaat
     2581 ttacattcct ccgggcgggc aggcaggttc agacggcatc gtcaggcaaa aggcggtgtc
     2641 ggaagacggg taaagaaggg gcgcgcaatc cggattattg atttatcgtg tttccttttc
     2701 ggttgaacct cagccctttg gggcggtaaa atcagacttt atttgggagg ggcgcaaccc
     2761 cttccgaatc aggacggcac atagggcggt gctttaatgt gtcgtcctgt gtgttgaaac
     2821 atattttctt tttattgggg agcttttatg aatatcaggt ttttcgcgct gaccgtatcg
     2881 gttttgtctt tggcggcctg tgccgtgccg gaagcgtatg atggcggcgg acgcgggtat
     2941 atgccgcctg ttcaaaacca agccggcccg gacgattttc gagcgttttc atgcgagaac
     3001 ggtttgtctg tgcgcgtccg caatttggac ggcggcaaaa tcgcgttgcg gctggacggc
     3061 aggcgtgccg tcctctcttc cgacgttgcc gcatccggcg aacgctatac cgccgaacac
     3121 ggtttgttcg gaaacggaac cgagtggcac cagaaaggcg gcgaagcctt tttcggcttt
     3181 accgatgcct acggcaattc ggtcgaaact tcctgccgcg cccgttaaac ggggcagcct
     3241 gtttgaaccg ccgtccggat ttttcgggcg gttttttatg ccggttttgt tttggccgtc
     3301 ggctggttcc ggcggttttc gggcgtggcg gattattttt cgtttcgttc tgggtttatc
     3361 ctgtcggcag cggctcaatg ccaactttaa acctgctccg atttcttcag ggctgttatc
     3421 caatgataaa attacatcgt ctgcatcaat ggcatcccac gcttccggct tgacatggcg
     3481 gctcgggctg attttcaggc agccgttgtg cagccaaata tccccgctca tcatgttttt
     3541 aaatagggcg cgtctggttt tatagcccaa gttcccgcat aggttggcaa cccaatcctc
     3601 atagcgttgc cgaaattttt cggtatcaaa aaaatcttgg tcttccgaac tgtcataaac
     3661 gaaagtcctg ctgtttgcca acgcttgcaa gaccgtcgtg cctaaagttt cattgtcggt
     3721 atccaatggc aggatatggg gggatatagg tggtctattg ccgttaaccc caaacctgaa
     3781 catgtttgaa caatcagagc ttcttcatta gcataaaaaa ctgcccaata attgatcctg
     3841 tttaaaaatc attatttaat ctccgtaatt ttgactgtaa tgttttgact tttgccatac
     3901 tctaccacgc gttgtaactg caatctttgc ttcttatata ttgtacccta tcaaagggct
     3961 gcattacttt tctttctaaa gattactatc gcataaaaag tatttttctt aaacgacagg
     4021 gatgcgcccg tgccatattc aaacaccgcc cggatgttgc gctgcccgat cggatgcttc
     4081 agacggcatc ggaagggttt gcagttttgg aatatgaaga tgataatgtc cgcgagattg
     4141 acggcatttg aaagcggtta tcccgtatac cggaggatac gaaatgaacg aatattccca
     4201 attaatcaag catcccgata tttccctttc cccgatttca gacggcatcg gggtcggcaa
     4261 tccggcgacg ggcgaaattt cggcgtatgt ccgcaacacg ggttcggaca agctgaaaaa
     4321 cctgattcaa aaggcggcgg cagcgcaaaa attatgggcg gcgaaaaccg ccttggaacg
     4381 cgccgacata ttgtggcgtt ggtatttttt agttaaagaa aacaaagaag cactggcgcg
     4441 tctcatgacg atggagcagg gcaaaagcct gaccgaggcg cgcggcgaaa tcgattatgc
     4501 ggcttcgttt gtgcgctggt ttgccgaaga ggcgcggcgg attgacggcg atgtgctgac
     4561 gagtgtgaaa gcgtcgcaaa aactggttgt gttgaaacag cccgtcggcg ttaccgctgc
     4621 gattacgccg tggaatttcc cgtccgcgat gatagtcaaa cctgcatcgc tcacgccttt
     4681 gagtgcgtat gcgttggctg tactggccta cgaagcgggc gtgccgcagg atttgcttcc
     4741 cgtcgtcagc ggccgcgctt cggaaatcag ccatgaattt gccacgaacc cgaccgtgcg
     4801 caaaatcagc ttcaccggct cgaccgaagt cggcgcgaaa atttttgccg gcagcgcggc
     4861 ggacattaaa aaactcagtt tggaactggg cggcaacgcg ccgtttatcg tgtttgacga
     4921 tgccgatttg gacaaagccg tcgaaggcgc gctggcgagc aagttccgca acagcggtca
     4981 gacctgcgtc tgcaccaacc gcgtttacgt ccaatcgggc atttacgacg tattttgccg
     5041 caaattgagt gaaaaggcag ccgcgctcaa attgggcgac ggcttggacg aaggtgtgaa
     5101 ccaaggaccg ctgattgagg aaaaagcggt agaaaaagtc gaacagcaca tcgccgacgc
     5161 actctccaaa ggcgcggtct gtctgaccgg cggcaaacgc agcgcgttgg gcggaacgtt
     5221 ttttgagccg actgtcttaa gcggcgttac ggcgcaaatg gcggtagctc gcgaggaaac
     5281 cttcgggcct ttgtgtccgg tgttccgttt tgaaaccgaa gccgaagtca tcgaggctgc
     5341 gaacaatacg gaatacggtt tggcagctta ccttttcacc tccgacaccg cccgtcaatg
     5401 gcgcgtcggc gaagtcttgg aatacggcat ggtcggcatc aatacgggct taatcagtaa
     5461 tgaagcggca ccgttcggcg gggtgaaacg cagcggtttg ggacgtgaag gcagcaaata
     5521 cggtgcggac gaatatctag aattgaaata tttgtgcatt gatgccgggt gacggatgcc
     5581 gtctgaatgg cagggtttca gacggtgtcg cattttaagc agtctctatc tgttgtacaa
     5641 tgcgccctgt ttttacggtt atttttgatt tgaatgtaag atatgatgga aaacggaaaa
     5701 acattcccga gatggcgttt tgccttgaaa agtgcgggct ggcacctctt aatcagccta
     5761 ttggttgcag ggctggcggc attgctggtt tttaaggttt ggtatcctta tccttatgcc
     5821 gagctgacgg gcggtctgtc gctttatcag ctggtggtgg cggtcgatat cgtatgcggc
     5881 ccgctgctga ctttaatttt ggcaagcccg aagaaaaaga caaaggcacg catggtcgat
     5941 ttttccatgg tcggcatcat ccagctggcg gctttggcgt acggtttgca cagcgtttcg
     6001 ctggcgcgtc ccgtggtgga agcgtttgaa caggaccgca tgaccattgt tacggcggcg
     6061 gaagtcgtgg tcgaagattt gcacaaagcc cccgaagggc tgcaaagcct gtcgtggttc
     6121 ggcatccgcc gcattgcatt gaaagaacct gaggatgcgg atgagaagaa caagacgctg
     6181 gatttgtccc tgaaaggtat cgagccgagt atgcgtcccg accggtggct gccgtattcc
     6241 gacaaggaag cagaaaaaat ccgcaaacat ctgaaaccgc tgaaagtctt ggcggatgcg
     6301 agaaaaacga cggttgcgga cattctgaaa caggcaggtc tcgccgaagg ggaggagctg
     6361 tattacctgc cgtttaccag cagcaggcag aaagagtgga tagtcattac cgataaagag
     6421 ggcaacacca aaggctacgc gccgatagac ggcttcatca tcccttaacc attgggattc
     6481 cgcccgcact cgaacatccg ttcttcgcgg cggtagaatc agactgtatt tgagaggggc
     6541 gcaacccctc aatcagggca acacataggg agacgcttta tgtgccgtcc gtgtattgaa
     6601 acatttcaaa ataagcccat gccgtccgaa catcctttca gacggcatgg cgttttgcca
     6661 ttgccccgat aagctgttaa actgtttaaa ttatttcgac cgaaggtaca cgcacatgaa
     6721 agaccccgag cagagcagta agcccgcccg ccgttttttg tgcgtaccgt ctgttcaggc
     6781 agcttgagcg tttcaggctg cctttttgcg tttgaacttg aggaacacga aaatcatgga
     6841 tttcagttgg ttggcagaac cgcatacctg gataggtttt gccacgcttt tggtgttgga
     6901 agtcgtattg gggatagaca accttgtctt tgtggcgatt ttggcaaaca aggtccagcc
     6961 cgcacggcgc gaccgcgcac ggattaccgg gctggggctg gcagtcgtca tccgcatcat
     7021 tatgcttgct tttatggcgc atatcatcac gctgaccgaa ccgctgttcc aaatcggcgg
     7081 cctcgccgtt tccggcaagg acatgattat gctcgcgggc ggtattttcc tgctttacaa
     7141 agccactacc gaactgcacg aacgcctcga agggcataac cggtttgccg ttgccgacaa
     7201 ccaaaaaaaa cacgcgccgt tttggggcgt ggtcgcgcaa atcctgatac tggatgccgt
     7261 gttttccatc gattcggtca tcaccgccgt ggcgatggtc gatcacatcg tcgtggcgat
     7321 gggcgcggtc gtcgtcgcaa tggttgtcat gatttccgcc agcaaactct tgaccgaatt
     7381 tgtggacagg catcccaccg tcgtgatgct ctgccttggt tttttgttga tgatcggttt
     7441 cagcctgatt gcggaagcct tccatttcca cattcccaaa ggctacctct acgccgccat
     7501 cggcttctcg attttaatcg aactgtttaa ccagatttcg caacgcaaca gccgcaaaaa
     7561 cgactacatc ggcagctcgt ggcgcaagcg caccgccgaa aacgttttgg gtatgatggg
     7621 catacgcgaa agcgtgcttg ctgacgcggg cggcgaatcc gtggatgacg cgcattttga
     7681 agaaaacgaa aaatcgatga tacgcagcgt gctgacgctt gccgaacgcc cgattatggg
     7741 ggtgatgatt ccacgccgcg acatcgaacg gctggacatt tcccaaagcc gcgaagaaca
     7801 gtatgcccaa ctgcaaaaca cgccttacag ccgcctgctc gttgtcggaa aggcgggcgt
     7861 ggacgaaccg ttgggctaca tcaacaaaaa agacctgctg tcccaactgc tggaaacagg
     7921 cggtctcgac attcagacgg cattgcgcca gccgctcgtc ctgcccgacg gcacgaccgc
     7981 cttgggcgcg ctcgaactct tccgccaaag cagcgcggat tacgctttgg tggtggacga
     8041 gttcggcgcg gtgttgggta tggtgacgat gaaagacctg ttggaagcca tcgcaggcga
     8101 gttccccgaa gaattcgagc gcgaagaaga acccgccgtt caggggaatc ccgatgaaag
     8161 cctgacggtg gaaggcgcgt tggaatatgt ggagctggca tcccaactca acctgccgca
     8221 gcaggaggaa gataccgatt tccataccgt tgccgggctg attatggaag aattgcaaac
     8281 catccccgat gtcggcgatt ttgccgattt tcacggctgg cggtttgaag tggtcgaaaa
     8341 agaagggcag cgcatcgagc gggtcaaaat caccaaattg cccgaagaat aagcatccag
     8401 gataaaaaat gaacgttttg atttccaacg acgacggcta tctggcagaa ggcattgcca
     8461 ttctggcgcg ggttgcgtcg gaatttgcca atgtcagggt ggtcgcgccc gagcgggaca
     8521 ggagcggggt cagcaattcg ctgacgctcg accgtccttt gcagctgaaa caggcgcaaa
     8581 acggcttctg ctacgtcaac ggcacgccga ccgactgcat ccatgtcggc cagtttgccc
     8641 tgcccgattt taagcccgat gtcgtgtttt caggtatcaa ccgtggcgca aatatgggcg
     8701 acgatacgct ttattcgggg acggttgcgg cggcgaccga agcctatctg atgggtatgc
     8761 ctgctgtggc gttttcttta aacgatgctt ccggacgcta ttgggcaacc gcagaaaagg
     8821 cactgtggac attgttggcg cattttttta aaaaaccgcc gtccgcgccc gtcttgtgga
     8881 atgtcaacat ccctgctgtc gcaccggaag atgtcagggg aatcaagatt acccgtttgg
     8941 gcagacggca tcatgagcag aacatcgtcc cgtcccgcaa tccgcgcggc gaacagattt
     9001 attggatagg gccggtcggc gaagtttccg atcgggaaga ggggacggat tttggtgaat
     9061 gcggcgcagg ttttattacc gtaacgccgc tgcaaatcga tttgaccgcc tatccggaca
     9121 tggcggagac ggcggccttt tggcatacgg actgaccgtt tttcaaatac cgtcgggttt
     9181 tgcgcttgag aagaacgcgc atttttatta tgatataccg cattcaggct atatatcgga
     9241 acatgaaggt tatcttatgt tgaaacaaac gacacttttg gcagcttgta ccgccgttgc
     9301 cgctctgttg ggcggttgcg ccacccaaca gcctgctcct gtcattgcag gcaattcagg
     9361 tatgcagacc gtatcgtctg cgccggttta caatccttat ggcgcaacgc cgtacaatgc
     9421 cgctcctgcc gccaacgatg cgccgtatgt gccgcccgtg caaactgcgc cggtttattc
     9481 gcctcctgct tatgttccgc cgtctgcacc tgccgtttcg ggtacatatg ttccttctta
     9541 cgcacccgtc gacatcaacg cggcgacgca tactattgtg cgtggcgaca cggtgtacaa
     9601 catttccaaa cgctaccata tctctcaaga cgatttccgt gcgtggaacg gcatgaccga
     9661 caatacgttg agcatcggtc agattgttaa agtcaaaccg gcaggatatg ccgcaccgaa
     9721 aaccgcagcc gtagaaagca ggcccgccgt accggctgcc gcgcaaaccc ctgtgaaacc
     9781 cgccgcgcaa ccgcccgttc agtccgcgcc gcaacctgcc gcgcccgctg cggaaaataa
     9841 agcggttccc gcccccgcgc ccgccccgca atctcctgcc gcttcgcctt ccggcacgcg
     9901 ttcggtcggc ggcattgttt ggcagcgtcc gacccaaggt aaagtggttg ccgatttcgg
     9961 cggcggcaac aagggtgtcg atattgccgg caatgccgga caacccgttt tggcggcggc
    10021 tgacggcaaa gtggtttatg ccggttcagg tttgagggaa tacggaaact tggtcatcat
    10081 ccagcacaat tcctctttcc tgaccgcgta cgggcacaac caaaaattgc tggtcggcga
    10141 aggtcagcag gtcaaacgcg gtcagcaggt tgctttgatg ggcaataccg atgcttccag
    10201 aacgcagctt catttcgagg tgcgtcaaaa cggcaaaccg gttaacccga acagctatat
    10261 cgcgttctga ctttccgttt gcaatcaggt taaaaatgcc gtctgaaagg tcttcagacg
    10321 gcatttttgt tttggcggct gtgttttcaa atcattccga agggacgggg cgtataaatt
    10381 tgtttgactg tgtttgggta tttgcttgat ttcatccgtt tgcaatggaa aagccgctta
    10441 tttccgttcg gagggcggtt ttatcggata aaaggcattt tgtccgactg attagggctg
    10501 catcaaggga atatgaaaga aaacacaacc gcttagtctc acggctgtgt tccaaaatgg
    10561 cgttgaaaaa cggatgagcc agaatcagcc gattttactt caataaatca aaccattaca
    10621 ccgaatgtcg gaacaatgct tgtcctgtct ttcagacggc ataggccgcc gtcagcagcc
    10681 gcaggacacg tctccggagg cttggaggct gatttctctg cgttttttgg ctttttcgta
    10741 gcggcagcgt tggcggtcgg tcaggatttc cagcggaggg acggggacgg gtttgccgtc
    10801 tttgaccgca accatcgtga agtagcagct gttggtatgg cggatttcgc cggtgcggat
    10861 gttttgtgct tcgacacgga tgccgatttc catcgaggtg cgtcccgtgt agttgacagc
    10921 ggcgtaaaag gtaaccaaat cgccgatgtg gatgggttct ttaaacagga ctttgtcaac
    10981 cgaaagggta acgcaataat tgccgctgta acggctggcg caggaatagg caacctggtc
    11041 gagcaggagc aggagttcgc cgccgtgtac gttgccgctg aaattggcgg tgtccggcat
    11101 catcagttcg gacataatga gttcgtggga aggcagttgg cgttgttgtg tcatggtgta
    11161 cctgttttaa gaaagggtgt tttgaaatgg gtgcgggaaa attttaggga atggggaagt
    11221 ggaattcaag ctaaaaaaag ctatggaggg atagtctgcc gaaatgggcc gccgatgccg
    11281 tctgaagcct tcagacggca ttttgttcag gaaagccaaa aacggaaaac caagaaaaac
    11341 acgagtgcca gcacgcccgc ccaaacggcg agcatgaatc cggcaaccgc ccgtcccata
    11401 cggtaaaccg gttttttgcg ttcgtcttcg atgggggagg cgtggtgttt ttcgggttgt
    11461 tcggacattt tgttctcaat cggaaaagtt ggaaacgggc gtaaggttac gctgccgtgc
    11521 aaaaagattt aagaaggaag gtcagcagct gtcttcccaa ttttcgtccc aagcgtgttt
    11581 caatgccgtc tgaacggtat cggcatcaaa accgcgatag gcgaggaaac gcgcctgttt
    11641 ttgtttttct ttgaggttgg cggcggggtg tttgaatttt ttacgcaaca cggctatggc
    11701 ggcttgcttt tcgcttgagc ggtcgggaag caggttgcgg ctggtttttt catctatgcc
    11761 ctgttgcgcc aaagcctgtt tcagcctcaa tgaaccgtgt ttgcggcttt tgctgcggat
    11821 ataggcttcg gcgtagcgca aatccgactg ccagttgcgt tcggcaaatt cgtttaacac
    11881 gttttccaac tcctcttcgc tttcggcgtg cggtgcaagt ttgcgtttca gaccgatgcg
    11941 gctgacttct tggcgcgaga ggatgtccat cgcgcgggcg cgtagggatt tttgcggttt
    12001 catggcggcg gtttcggcac ggaaagattc agacggtatg ggaaacggat tccgatgccg
    12061 tctgaacaga ttgttttatt cttcgtcttt gtttttctgc ggtcgcaggt tttcgtaaat
    12121 ttcgggcagc gcgccgataa tccggtcggg gcgggtcgta tcgtcttgcg agagcagcgt
    12181 catatcgccg taaccgaagg taacgccgac actcaggcag ccggcggctt tggcggcgat
    12241 gatgtcgttg cgcgagtcgc cgaccataag catattggcg gcatcgatac ccaaaacttc
    12301 ggcggcgtgc cgcagcggca gcgggctggg ttttttttcg ggcaggctgt ctccgccgag
    12361 tatcaggctg aagtagtcgg cgagtcccag ttgttttaga agctcggcgg caaggatttc
    12421 gtttttgttg gtaatgatta ccagcggaat gcccaaggat ttgagcaatg ccagcccggc
    12481 ttcggtttcg ggataggggc gggtgaagac gctcaaatgg tcgcggtagt atttcatata
    12541 ggacacgaaa cctttttccc acagttcgga atcggcttcg cggtcgcggt cgttggtgag
    12601 gacgcggtga accagtttgc cgatgccgtc gccgacgtag ctttcgacca ctttggcggg
    12661 cagcggtttc ataccgagtt gttccaacat cgcttctgcg gcggcggcaa ggtcggggac
    12721 ggaatcgcac agtgtgccgt ccaaatcgaa ggcgacggct tggacgtgtt cgatggctgc
    12781 attcataatc cggtttcctg tcgggttgaa agggtttatt ttaacatcgt ttgcacaggg
    12841 cttctaatgt atagattatt aacaatcgca acaggttttt cgggcggtgt tttcggattt
    12901 tatggcgggg tctggcaagg gtttgaaatc ccggcgatgt tccaattaaa tttttaaaaa
    12961 ataaaatgat gactatatct ttatttaatt ggacatttgc ttttcggatg cgcgctgctt
    13021 ttttcagacg gatgctgtgc ggcttgaaaa ataaaatcat atatagatta ataaaatcgg
    13081 gcttatgtcg actacatgct attacgtttt gtcgggaaat tggttatagt aatgagcgaa
    13141 atgcacgccc ctgaaaatct ttttgcgaaa aggaagcaaa atgtctgatg ccgttgcaaa
    13201 agaaaccctc aatccgtttg aaatcgcgcg aaaacaggtc aaaaccgcct gcgaccggct
    13261 gaaaaccgat ccggccgttt atgaaatcct gaagagtccg acccgcgtgt tggaagtcaa
    13321 actcgacgac ggcacggtca aaacctttac cggttaccgt tcgcgtgcgt tcccatcccg
    13381 gtgtgaatct ggacgaagtc aaggcactgt cgatttggat gaccatcaaa tgctgcgttg
    13441 caggcattcc ttacggcggc ggcaaaggcg gcgttacctt ggatccgcgc gattattccg
    13501 aggcggaact ggaacgcatc gcccgagctt atgccgaagc gattgccccg ctgatcggcg
    13561 aaaaaaatcg atattcccgc ccccgatgtg aacaccaacg gcaaaattat gtcgtggatg
    13621 gtggatgcct atgaaaacgt ggtgaaacat tccgcgccgg gcgtatttac cggcaaaccg
    13681 gttgagttcg gcggctcttt ggcgcgtacc gaggctaccg gttacggcgt gaacctcgcc
    13741 gccgtccaag ctttggaaaa actgggcaaa gatgtgaaag gcgcaaccta cgccattcaa
    13801 ggtttcggca atgtgggcta ccacaccggc tattacgcgc atcaatccgg cgcgaaagtc
    13861 gttaccgtat ctactgttga cgttgctatc tacaatgaaa acggcttgga tatggaagcg
    13921 ctgtttaaag agttccaaga aaaaggcttc atcaccaaca aagccggtta cggcaaagaa
    13981 attaccaacg ccgaactttt ggctttggat atggacgtac tcgccccttg cgcattggaa
    14041 aaccagctga cttccgaaaa cgccggtaaa gtccgcgcga aaatcgtggt tgaaggcgca
    14101 aacggcccga ctacgcccga agccgacgtt atcctgcgtc aaaacggcgt attggtcgtg
    14161 cccgatattt tggcaaactg cggcggcgtg gtcgtttcct atttcgagtg ggtgcaaaac
    14221 ctgcaaggct attattggga gtttgacgaa gttcaggaaa aagaaatcgt cgtcctgcgc
    14281 cgcgcgttcc gcgatatttg gaacttggcg caagagtatg atgtcgattt gcgtaccgcg
    14341 tcttatatga tgagtatccg ccgtgttgaa aaagcgatga agctgcgcgg ctggtattga
    14401 tgtttgaaca aaggatgccg tctgaaacca tttcagacgg catttttgtt tggacggaat
    14461 taatggtgag aatggccgat ttggaaggtt aaagttgtgt agttcgcctg tttttggcac
    14521 aggctttgat cggggaaatc ggctttgtat tcgacactcg ctttccaaaa gccttggcgc
    14581 aaggggatga tgtccacttc gcctttgccg tcggtggtgt cggagaaggc ttgggcttcg
    14641 gttttgtgcg ttttgctgcg gtcgctggtg tcgaagccgt caaatgtagc ggtaacggtg
    14701 gcattgggca gcggttcgcc acggaacaga acgcggactt tgaagcgttc gcctacgtga
    14761 atgttggcgg gattgtccag cgggacgatt tccaagtttt gtccgaccgg tttggtgatg
    14821 atggcggtgt ccgcgctttc gtgtcccacg ttgacaatgt ttttaccgaa catacgggtt
    14881 tgttcgcaat agcttgcgtc aggcatttct ttgatgccag cctgtttcca gcctgctttg
    14941 ttttttgacc ggaaagtagg ctgatattcg gcggtaacga ggtagctgcc gtctttgacg
    15001 ggacggttgc tgcggtattg gtagttgtat gtgccgcgtt gaatcatgtt ttccttacct
    15061 ttttcggtaa ccagctgcat cggtttgctg aagatgtgca ggcggtcttt ggcgatgggt
    15121 tcgagttcgg ggaattcgcc gtagcccaag tcggctttaa ggtattcgcc gccgtgcgtg
    15181 tgggcggttt cgacccagac gcggtgggcg tgtgcggatg tggcaaatag tgcggaaacg
    15241 gcgagcagtg tcaatgtttt cttcatggtg cagcctttat atcgtgcggg ttaaaaattt
    15301 tgttatgcta taacaaaata tgtatcgtgt gaagacggat ttgccgcctg aaaactgtct
    15361 ttttgtatcg atttgaattt ttcagaaaat gaagcggacg tttgggcgga ggcaacttgt
    15421 ttgataagat agcaatattt caaaacggag aaatatcatg ccttatgtca atattaaagt
    15481 aaccggcggc aaggaagcac cgactgccgc gcaaaaagcg gaactgatcg gcggcgtaat
    15541 cgaattgctg gcacgcgtgc tgggtaaaaa tcccgaaaca acggttgtcg tgattgacga
    15601 agtggatacc gataactggg atataggcgg caaaagcgtc agcgaacggc gcaaagaggg
    15661 caggtaaaaa gcctgaaaac cccgccctga tgctttaaat ttcgcgtgaa aaagagtaca
    15721 ttcccaccca ttgcccaaaa tttacggaac acattatgga taaatttccc aagtctgcaa
    15781 agctcgacca cgtctgctac gacatacgcg ggacggttca caaaaaagcc cttcagttgg
    15841 aagaggaggg caacaaaatc ctcaagctca atatcggcaa ccctgcgccg ttcggctttg
    15901 aagcacctga tgaaatctta gtcgatgtca tccgcaacct gccgacttcg caaggctatt
    15961 gcgattcgaa ggggttgtat tccgcacgca aagccattgt tcactactat cagaacaaag
    16021 gtttgcgcga tattacggta aacgatgtct atatcggcaa cggcgtgtcc gagctgattg
    16081 cgatgtctat gcaggcgttg ctcaacgacg gcgacgaaat cctgattccc gcgcccgact
    16141 accctttgtg gacggcggcg gcaacgcttg cgggcggtac ggtacgccat tatctgtgcg
    16201 acgaagaaaa cggctggttt cccaaccttg ccgatatgga agccaaaatc acgtccaaaa
    16261 ccaaagccat cgtcgtcatc aaccccaata acccgacagg agcggtgtac agcaaggaaa
    16321 tcctgctgga aattgccgaa ctggcgcgca agcatggttt gattattttc gccgacgaga
    16381 tttacgacaa aatcctttat gatggcgcgg ttcactgcca catcgccgcg ctcgcccccg
    16441 accttttgac ggtaacgttc aacggtttgt ccaaagccta ccgtgtagcc gggttccgcc
    16501 aaggctggat ggtgctaaac gggccgaaac atcacgcaaa aggttatatc gagggtttgg
    16561 atatgctctc gtccatgcgc ttgtgtgcca atacgccgat gcagcacgcg attcagacgg
    16621 cattgggcgg ttatcagagc attaacgaat tcatcttgcc cggcggacgg cttttggaac
    16681 agcgcaacag agcgtgggaa ctggtcaacc agattcccgg cgtgtcctgc gtcaaaccga
    16741 tgggcgcgat gtatatgttc ccgaaaatcg ataccgaaat gtaccgcatc cgcgatgata
    16801 tgaaattcgt ttacgatttg ctggtgcgcg aaaaagtcct gctggtacag ggaacggggt
    16861 ttaattggat caaacccgac catttccgca ttgttacgct gccttacgtc catcagattg
    16921 aagaggcgat gggcaggttg gcaagattcc tgcaaaccta ccatcagtag gattctgata
    16981 aaaaatgccg tctggaacgg agattcccgt ttcagacggc attttcaaca gcaggaacaa
    17041 atcaggcaaa tttcagtctg tcgccgtcgg cttctacctt gatttcgctt tcgggcgcat
    17101 agtttccggc aagcagggct ttggccagcg ggttttcgat ttccgattgg atcgcgcgtt
    17161 tgagcggacg tgcgccgtaa atcgggtcga aaccggcttt ggcgatgatg tccagagcgg
    17221 catcggaaac agtcaggcgc aggttttgtt tttccaaacg tttttccaaa cctttgagct
    17281 ggattttcgc aatactgcgg atattggcct gatccaagcc atggaacacg accacttcgt
    17341 cgatgcggtt gatcatttcc ggacggaaat gttctttcac atcctccatc acaacttctt
    17401 tcaccgcttc gtagtcctgc gtgcccattt gttggatgtg ctggctgccg atgttggaag
    17461 tcatcacaat cacggtattt ttaaaatcta ccgtcctgcc ttgtccgtcg gtcaaacgac
    17521 cgtcatccaa tacttgcagc aggatgttga atacgtcggg atgggctttt tccacttcgt
    17581 ccaaaagaat cacgctgtac ggtttgcggc ggacttgttc ggtcaggtag ccgccctctt
    17641 cgtatccgac ataacccgga ggcgcgccga ttaagcgggc aacggcgtgt ttttccatgt
    17701 attcggacat gtcgatgcgg atgaggtggt cttcgctgtc gaacaggaag cctgccaaag
    17761 ctttgcacaa ctcggtctta cccacaccgg tcgggcctaa gaacaggaag ctgccgtaag
    17821 gtttgttcgg atcggcaagg ccggagcggc tgcggcggat ggcatcggac acggcgcgca
    17881 cggcttcgtt ttgaccgact acgcggcggt gcaacacttc ttccattttc aacaatttat
    17941 cgcgttcgcc ttccatcatt ttggatacgg gaatgccggt catacgggaa acgatttcgg
    18001 ccacttcatc cgcgccgaca ttattacgca agagtttgtt tgccggtttt gtgctgtccg
    18061 tatctgcccg ttcggcggct gcacgctgtt tttccaaatg ctccaaatct tcatacatca
    18121 attttgaagc cagtgccaaa tcgccttgcc gttttgcctg ttcgatttta attttgactt
    18181 cgtcaatttg tttcttaata ttggcagcac cgtctgaaat cgctttttcg gctttccaga
    18241 tttcgtccaa atcggcgtat tctttttgca agccgtcgat ttcctcgtct atcagttcca
    18301 aacgtttttt gctggcatcg tctttttctt tttcaacgtg cgccttttcc atccgaagct
    18361 gaatcaaacg gcggtcgatt ttgtccatag cttccggttt ggtttctttt tccatcttga
    18421 cacggctggc agcttcgtca atcaaatcaa tcgccttgtc gggcaaaaag cggtcggtaa
    18481 tgtagcggtc gctcaactcc gctgcggcaa caatggcggg gtcggtaata tcgatgccgt
    18541 ggtggatttc atagcgttcc tgcaaaccgc gcaggatagc gatggtgtct tccacgctgg
    18601 gttcgccgac caatactttt tggaaacggc gttcgagtgc cgcgtctttt tcgatgtatt
    18661 ggcggtattc gtccaaagta gtcgcaccga tacagtgcag ttcgccccgc gccaaggccg
    18721 gtttcaacat attgcccgcg tccatcgcgc cgtcggtttt gcccgcgccg accaaagtat
    18781 ggatttcatc gatgaaaatc agggtgttgc cgtcgtcttt cgccaaatcg ttcaatacgc
    18841 ctttcaagcg ttcttcaaat tcgccacggt atttcgcacc ggcaatcaaa gccgccaaat
    18901 ccaaaaccag caggcgtttg ttacgcaggg attcaggtac ttcgccgttg acgatgcgtt
    18961 gcgccaaacc ttcgacgatg gcggttttac ccacaccggg ttcgccaatc agcacggggt
    19021 tgtttttggt gcggcgttgc agcacctgaa tcgcgcggcg gatttcatca tcacgaccga
    19081 taacggggtc gagcttaccg tctcgggcgc gttgggtcag atcgagcgtg tattttttca
    19141 aagcatcgcg ttggtcttcg gcattcgcat cgttcacgtt ttgtccgcct cgtaccgcat
    19201 cgatcgcggc attgatgttt tgttcggtcg caccggcttc tttcaagatt ttgccggccg
    19261 catcgttctg ctgcaccaag gcgagcagga aaagttcgct ggcgatatag gcatcgccgc
    19321 gtttggttgc cgctttgtcc atcaggttca acaccgcctg caattcgcgg ctgggcagaa
    19381 tctcaccacc ttgtccggac actttcggca ggctgttcaa atgctgctgc aaacgctgtt
    19441 taacctgcgg cacgttcacg cccgcatgag ccaagagcgc ggcagctccg ctgttttggt
    19501 cgtcaagcag ggctttcagt acgaaacctg cttccagata gctgctgtcc gcagccaacg
    19561 ccaaactctg agcttctgca agggcttgtt ggaatttggc ggttaatttg tcgtatcgca
    19621 ttttcgtttc cttttcaaaa tgtccgctgt cgaagcctat atgtgcatga ctgtggataa
    19681 ctcaagtgtt gttttctgtt tttctatatt taattcgata tattgttgaa tttaaagtat
    19741 atagaaatgt atgataatat gtataactat atcttcttaa tatggaaaag tctgttgtcg
    19801 gctggatgta tgtggcaaat cgggtataat cggcgcatct tttccctttc agacggcatt
    19861 gctgccgtaa ggacattttt atgagcaaaa aacgagttct gaccggcgta accaccaccg
    19921 gcaccccgca tctgggcaac tacgtcggcg ccatccgccc cgccgtccgc gcggcgcaaa
    19981 accccgatac cgaatccttc ctcttcctcg ccgattacca cggcatcatc aaatgccacg
    20041 agcaggagat gattcaccaa tccacccaag ccgttgccgc cacttggctt gcctgcggac
    20101 tcgaccccga gcgcaccacc ttctaccgcc aaagcgacat tcccgaagtg atggagttga
    20161 actggattct gacctgcatc accgccaagg gtttgatgaa ccgcgcccac gcctacaaag
    20221 ccgccgttca agccaatgcg gaaaacgggc aggaagaccc cgatttcggt gtggaaatgg
    20281 gtttgttcag ttatccgatt ctgatgactg ccgacatttt gatgttcaac gccaacgaag
    20341 tccccgtcgg gcgcgaccaa atccaacacg tcgaaatggc gcgcgacatt gccggccgct
    20401 tcaaccaccg tttccaagaa ctcttcaccc tgcccgaagt gaaaatcgac gaaaacgtcg
    20461 aactcttggt cggtttggac ggacgcaaaa tgtccaaatc ctacggcaat accattccgc
    20521 tttgggaaaa cgacaaaaaa acccaaaaat cggtcaacaa aatcattacc aacatgaaag
    20581 agccgggcga gccgaaacag cccgacgaaa gccctttgtt tgaaatctat aaagccttct
    20641 ccacgccgtc tgaaacggcg gaatttacgc aaatgcttgc cgacggcttg gcttggggcg
    20701 aggcgaaaaa actttcggcg gcgaaaatca acgccgaact ggcagaactg cgcgaacgct
    20761 acaacgcatt gacctctaat ccttcgcaaa tcgaagagat tttgcaggca ggcgcgcaaa
    20821 aagcgcgcaa agaagcgcgc gaattgttgg ataaagtacg cgatgcggtc ggcatccgcc
    20881 cgttgaaata aacccaatgc cgtccgaacc tccgcctgcc acgtttcaga cggcatttca
    20941 gacggcattt tgaaaccatc aggagtgcgg aatgctgaaa ataccttttg ccgtgttggg
    21001 cggctgcctg ctgcttgccg cctgcggcaa atccgaaaat acggcggaac agccgcaaaa
    21061 tgcggcacaa agcgcgccga aaccggtttt caaagtcaaa tacatcgaca atacggcgat
    21121 tgccggtttg gctttgggac aaagtagcga aggcaaaacc aacgacggca aaaaacaaat
    21181 cagttatccg attaaaggct tgccggaaca aaacgccgtc cggctgaccg gaaagcatcc
    21241 caacgacttg gaagccgtcg tcggcaaatg tatggaaacc gacggaaagg acgcgccttc
    21301 gggctgggcg gaaaacggcg tgtgccatac cttgtttgcc aaactggtgg gcaatatcgc
    21361 cgaagacggc ggcaaactga cggattacct gatttcgcat tccgccctgc aaccctatca
    21421 ggcaggcaaa agcggctatg ccgccgtgca gaacggacgc tatgtgctgg aaatcgacag
    21481 cgagggggcg ttttatttcc gccgccgcca ttattgagtt attcggacat cacggaatat
    21541 atttggattt ttcaaacctt gcggaaaaag tccgcgccgc cgggaaactc aaaaggaggg
    21601 gatgtgtgtt acaattttcc gaactgtttt cataaaatag ttttcggacg tgtttcaata
    21661 tggcattgat gccgccttgt tgttcgagaa aaaaaccttt atatttaata taatgggttt
    21721 taactaaacg ggaaaccgtt ttctctccgg ccgatgggca aaatcagccg attgatggaa
    21781 cacggtccgg tttttaagca aaaccttaat caacaactca agaaggataa gacatgaaaa
    21841 aattattgat tgccgcaatg atggcggctg ccttggcagc ttgttcgcaa gaagccaaac
    21901 aggaggttaa agaagcggcc caagccgttg agtccgatgt taaagacact gcggcttctg
    21961 ccgccgagtc tgccgcttct gccgtcgaag aagcgaaagg ccaagtcaaa gatgctgcgg
    22021 ctgatgcaaa ggcaagtgcc gaggaagctg taactgaagc caaagacgcg gcagccgaaa
    22081 ccaaagaagc ggtaagcgaa gcggctaaag acactttgaa caaagctgcc gacgcggctc
    22141 aggaagcggc agacaaaatg aaagacgccg ccaaataatt tgttgccttg gcaaaaatga
    22201 cgagatgccg cctgccggca acccaaatga aaccgcctga agattttcag gcggtttttg
    22261 agttgtatgc cccttgtttt ttacgcctgc atgaccgtgc agggaggatg tgccgcgcat
    22321 atcgggatat tgcggacagg gaaatgccgt ctgaacatca tttagacggc atttggtttg
    22381 tcaaggttca gacggcttca actttgaacg gcatattgat tttttgccat tggacgcttt
    22441 catattccaa tgcgtcggca atcagggggt ggcgttccag ccatttcctg tcaatacgca
    22501 ggatgaagcc gcagctttcc gtatccgtac gcaactgcat atttttcggg aaacccagat
    22561 cttggcgcga acggcagaac agggcggcaa ggcgcaggga caaaacggca taccacaaca
    22621 tttcgttgcc gccgataatg ccgcccattt ttttcatatc gccgcgatga ccgatgacca
    22681 gttgggcaag tatggtctgt tccttgcgtg aaaaacccgg catatcggcg ttttcgagga
    22741 tgtaggcgga atgcttgtga tagccggtgt gggcgatgtc caaaccgatt tcatgcagcg
    22801 cggcggcgcg tccgagatac tgttgccaca aggcaagttc ttgaaccgta acgtttttgg
    22861 catgacagag gctgtccata aaggtttgcg cggtctcggc ggtgcgtttc gcctgattga
    22921 ggctgacgtg gtagcggtgt tggaactcgg caaccgtttg tccgcgcata tcttcgttta
    22981 agccgcgccc gatcaaatcg taaaacacgc cgtcgcgcag ggcggcttcg gttacggtca
    23041 tcctgtcgag tttcatttcc tcaaacgccg ccatcatcac ggcaagtccg ccggcaaaaa
    23101 cttcgatgcg ttccggtttc aggttttcaa atttggcttt tttgaccgaa ccggcttcga
    23161 tgatgcgttc ggcgagggcg cgcattccct tgtaggtaat gtccgcctct tggggcattt
    23221 cggcggcaag cacgtcgcgg atggattttg ccgaacccga tgtgccgacg gcgaaatccc
    23281 aaccttcgcg cctcatattt ttgctgatgc gctggatttc gttgcgggcg gcggaaatgg
    23341 cggcttggaa gtctttggcg gtgattttgt tttggaagaa gcgcaggctg taggttacgc
    23401 agcccaaggg caggctttcg gtaatgtcgg gattcagcgt cgaaccgatg acaaattctg
    23461 tcgaactgcc gccgatgtcg ataaccagca ttttgccccc gcaggggggg agggtgtgga
    23521 tcacgccggt ataaatcagc cgcgcctctt cgcgcccggc gatgatttcg atggggaaac
    23581 ccagtgccgc ttcggccttg ggaaggaaat ctgcgatgtt tttggcgacg cggaatgtat
    23641 tggttgccac ggcgcgcacc tgctcggggc ggaagccgcg caggcgttcg ccgaattttg
    23701 ccaggcagtc caaagcctgt tcctgcgagg atgtgctgag gtttttctgt tcgtccagac
    23761 cggcggcaaa acggaccatc tgtttgaagg aatcgatgac ttttaattgt ccgttgttgt
    23821 tttcgcaaat ctggaggcgg aaactgttgg aacccaaatc gacggaggcg aggacgtttg
    23881 cgggattggt ggtcatggcg gataccggtg ggggaaaaac gcaatgttac tctgacggcg
    23941 caggcgttga caataaatga tgcggctgtt tttgattctg ctcacggatg ttgccgacgg
    24001 tatgtttccg acagcaaaaa atgccgtctg aaaaacgttc ggacggcatt ttttgcgctt
    24061 atttgaaatc cttttccacg ctcatgaaaa tctgcgtatt tttgcgtgtg taaaaacttt
    24121 tcatattgct gtcgattttc agatagcgga aattgagttg cggcgtaaag cccttccaag
    24181 agattttgtc gtgccgcaac gacaggtttg cctgatattc gtggtctttg cgcgggaagc
    24241 ggtacacaat tgtcccgggt gcatcaaaag tcctgcgggt atagcgcagg tttgcccgca
    24301 gccccaagcc gccgtcgaac gttttgaccg cgccgacacg caaacccttg cggacggaag
    24361 cctgttccgc ctctttcgtc atgtcgtgcg gccagtccgc accgccgtaa agcagccggt
    24421 ctttcggcgc ggaatacatc agcgtcgcgc ccgccagcgg catatggctg ccgtatcggg
    24481 cggcggtgcg gtcttcctga taatgcttcc acatattgcc cgcgttcagc gtcagccgcc
    24541 agcgttcgcc caagcgtcgg gagaaatcgg cattgaagcc gccggcgaaa ttgtatcggc
    24601 tgccgcccaa gaggttttgc ccgacaaacg gcacggtgcc gaacgagcgc gttaccgaac
    24661 ggttttgatg gccgaacgac aggcgcaggc tctgttcgct gaaatctttg ttatcccaat
    24721 aatgcacgcc gcggctgatg ccgccgtaga ggaaatgatg cccgcccgca ttgatttcgc
    24781 gcgacacgcc caagccgtag cgcaaaccgt gtgccttttg cggcaggctg tcggcggttt
    24841 tcgtccagct tctgcccgca aattcaatcg ttttttcgga cgaagcgtt
//
LOCUS       DAXDXW010000023        24062 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXDXW010000023 DAXDXW010000000
VERSION     DAXDXW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24062
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.023"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            33..1919
                     /gene="mafB"
                     /locus_tag="ACFB64_000833"
     CDS             33..1919
                     /gene="mafB"
                     /locus_tag="ACFB64_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFC1209224.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDPFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPVKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHAQREWENKTGLDFNHF
                     IGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIKKPDGSWEVKTKKGG
                     KVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTSKSGIKIEGFTEPNRTAY
                     PIYE"
     gene            1950..2369
                     /locus_tag="ACFB64_000834"
     CDS             1950..2369
                     /locus_tag="ACFB64_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209225.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            2459..2530
                     /locus_tag="ACFB64_000835"
                     /pseudo
     CDS             2459..2530
                     /locus_tag="ACFB64_000835"
                     /inference="COORDINATES: protein motif:HMM:NF026862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 17 domain-containing
                     protein"
     gene            2527..2769
                     /gene="imm31"
                     /locus_tag="ACFB64_000836"
     CDS             2527..2769
                     /gene="imm31"
                     /locus_tag="ACFB64_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm31 family immunity protein"
                     /protein_id="HFC1209226.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            2847..3092
                     /locus_tag="ACFB64_000837"
     CDS             2847..3092
                     /locus_tag="ACFB64_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC1209227.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLIREGLHHDTGHIGWRAMSKGK"
     gene            3095..3485
                     /locus_tag="ACFB64_000838"
                     /pseudo
     CDS             3095..3485
                     /locus_tag="ACFB64_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3525..4982)
                     /locus_tag="ACFB64_000839"
     CDS             complement(3525..4982)
                     /locus_tag="ACFB64_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC1209228.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHALATL
                     SLGGFSTHDASIGYYNSPLIEAVIIVFTIFGGINFASHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGHYAGFGESLRYTAFNFVSIGLANGLSNTDFAQWPLLIS
                     LWMFFLANILASSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(4997..5827)
                     /locus_tag="ACFB64_000840"
     CDS             complement(4997..5827)
                     /locus_tag="ACFB64_000840"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC1209229.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLYLLAVGCGEGALKRSDTIEPILKHPDGGKMLDWL
                     RAQPLLIREGGRVMIHAGILPQWRIAKAESLAGEAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     HTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(5904..6293)
                     /locus_tag="ACFB64_000841"
     CDS             complement(5904..6293)
                     /locus_tag="ACFB64_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC1209230.1"
                     /translation="MSKTVIHTDKAPAAIGAYSQAVRAGGTVYMSGQIPLDPATMTVV
                     GNGDFHTEARQVFQNLQAVAEAAGGSLDDIVKLNAYLTDLGNFAVFNEVMAEFITEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(6440..6967)
                     /gene="nspA"
                     /locus_tag="ACFB64_000842"
     CDS             complement(6440..6967)
                     /gene="nspA"
                     /locus_tag="ACFB64_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC1209231.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKQAPSTDFKLYSIGASVIYDFDTQSPVKPYF
                     GARLSLNRASAHLGGSDSFSKTSAGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVK
                     NVRSGELSAGVRVKF"
     gene            complement(7410..8585)
                     /gene="hemW"
                     /locus_tag="ACFB64_000843"
     CDS             complement(7410..8585)
                     /gene="hemW"
                     /locus_tag="ACFB64_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC1209232.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYSLPNQTVQTALDDVQTAIATDATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGTLEGAGFIHYETSAFAKPTMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPGEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPAAKIMVQIETARQKGLLETDPTVFRPTEKGRLFLNDLLQCFL"
     gene            complement(8642..11113)
                     /gene="ligA"
                     /locus_tag="ACFB64_000844"
     CDS             complement(8642..11113)
                     /gene="ligA"
                     /locus_tag="ACFB64_000844"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC1209233.1"
                     /translation="MNPTAQRIHELTDLLNRYAYEYYTLDAPSIPDAEYDRLFRELEA
                     LERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPMLSLTNAFSPQDENGVFDHAEMYA
                     FDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVLVQAATRGDGTTGEDVTRNVKTV
                     SNIPLRLHGENVPELIEVRGEVLMLKADFAGLNKRQAENGQKPFANPRNAAAGSLRQL
                     DSRITAQRKLHFFPYSIARQQGGFEAEEHIQELAYFQELGFSLPNGNFGCFKNIGEVL
                     AFYEHMQQKRPELPYEIDGMVVKVNSLAQQRELGFISRAPRWAVAHKFPAEEALTIVE
                     AIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQDEVSRKDVRVGDTVVVRRAGDV
                     IPEVVRVIFERRPMQETAVAVSDGIGHQQDDLFAETPSAKQTESVPLHKPYRLPARCP
                     ICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFASRKAMDIDGLGEKQIEQLVAQDL
                     VRHFADLYRIDIPTLQKMKETADKGSSENENGDAETVSGDLSKYNTQNGKKQPTKWAQ
                     NILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGTLERVRRAPEPVLACLPDI
                     GTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPAAQYAGPQRWITRLPGFKI
                     SENKAQALWELAGQSIEGLQNDKALPADWQAWRSKAQNTALLENLKTFFAQMPSEDEA
                     AQGSDGINKAVAGKTFVLTGTLPTFKRDQAQALIEAAGGKVSGSVSKKTDYVVAGEAA
                     GSKLEKANALGVSVLSEAELLTLLC"
     gene            complement(11247..12533)
                     /locus_tag="ACFB64_000845"
     CDS             complement(11247..12533)
                     /locus_tag="ACFB64_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC1209234.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKPAKPQDSAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEEIGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDMRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQADAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(12727..13299)
                     /gene="ampD"
                     /locus_tag="ACFB64_000846"
     CDS             complement(12727..13299)
                     /gene="ampD"
                     /locus_tag="ACFB64_000846"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC1209235.1"
                     /translation="MDNHAGAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLHVSSHFLIARDGETVQFVSCGDMAYH
                     AGASSFRGREKCNAFSIGIELEGCDFEPFAEAQYRSLETLLAALCRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFPVDRNAV"
     gene            13383..14378
                     /gene="mltG"
                     /locus_tag="ACFB64_000847"
     CDS             13383..14378
                     /gene="mltG"
                     /locus_tag="ACFB64_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC1209236.1"
                     /translation="MLKKMLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIGHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     HEIDAGGSDLQIYQTAYKAMQRRLNEAWAGRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            14438..15058
                     /gene="tmk"
                     /locus_tag="ACFB64_000848"
     CDS             14438..15058
                     /gene="tmk"
                     /locus_tag="ACFB64_000848"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC1209237.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEEVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQAREKDRFEQEQAD
                     FFMRVRGVYLDRAAACPERYAVIDSNRSLDEVRNSIEKVLDGHFGC"
     gene            15280..16560
                     /locus_tag="ACFB64_000849"
     CDS             15280..16560
                     /locus_tag="ACFB64_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HFC1209238.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRYTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGRRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLGEWKL"
     gene            16962..17993
                     /gene="lpxK"
                     /locus_tag="ACFB64_000850"
     CDS             16962..17993
                     /gene="lpxK"
                     /locus_tag="ACFB64_000850"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC1209239.1"
                     /translation="MPNFFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKWRADF
                     LSGKRQSEKLSVPVVVVGNIHAGGTGKTPIAAALVSGLQEKGVKVGIISRGYGRKSKA
                     VYVLNAASRAEDAGDEPLLLFRKTGAPTAVGSSRVEAGRALLAAHPELELIVADDGLQ
                     HYALQRDVEIAVFPAADTGRTDLDLLPNGNLREPLSRLESVDAVVVGGRAADGFMPSE
                     HLFGSRIEAGAVYRLNRPSEKLDISTLSGKRVAAVAGIARPQRFFDTLTHMGIRLDQT
                     VALPDHADIFNRDLPPADVVLVTEKDAVKFSDGICTDNVWVLPVCAIIEPDLAEFVLE
                     RLEGVPKAV"
     gene            18199..18732
                     /locus_tag="ACFB64_000851"
     CDS             18199..18732
                     /locus_tag="ACFB64_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFC1209240.1"
                     /translation="MKLKTLLLPFAALALCANAFAAPPGDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PEFTEELRRIICGGIVD"
     gene            18717..18824
                     /locus_tag="ACFB64_000852"
                     /pseudo
     CDS             18717..18824
                     /locus_tag="ACFB64_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            18954..19136
                     /locus_tag="ACFB64_000853"
     CDS             18954..19136
                     /locus_tag="ACFB64_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC1209241.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            19133..19894
                     /gene="kdsB"
                     /locus_tag="ACFB64_000854"
     CDS             19133..19894
                     /gene="kdsB"
                     /locus_tag="ACFB64_000854"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC1209242.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTACQAHGIEVVMTSNRHESGTTRLAEAAAALKLPPHLIVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAGHELHDFDELMNPNAVKVVLDKNGNAIYFSRAP
                     IPYPRDAMRAGKREMPSETAVLRHIGIYAYRVGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            19917..20432
                     /locus_tag="ACFB64_000855"
                     /pseudo
     CDS             19917..20432
                     /locus_tag="ACFB64_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            20589..20681
                     /locus_tag="ACFB64_000856"
     tRNA            20589..20681
                     /locus_tag="ACFB64_000856"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20623..20625,aa:Ser,seq:gct)
     gene            20955..21740
                     /gene="trpA"
                     /locus_tag="ACFB64_000857"
     CDS             20955..21740
                     /gene="trpA"
                     /locus_tag="ACFB64_000857"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC1209243.1"
                     /translation="MSRIRQAFAALDGGKALIPYIAVGDPDIRTTLALMHGMVASGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PIHKMGYREFAQEAAKAGVDGVLTVDSPIETIDSLYRELKDNEVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLRQYIDIPIGVGFGISNAESA
                     RKIGRVAAAIIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            21777..22649
                     /gene="accD"
                     /locus_tag="ACFB64_000858"
     CDS             21777..22649
                     /gene="accD"
                     /locus_tag="ACFB64_000858"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC1209244.1"
                     /translation="MSWLDKILPPKIKNRGKDGSSNVPEGLWRKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEEGREEVAGNVKPTDPLKFKDSKKYPDRLSAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAT"
     gene            complement(22709..23320)
                     /locus_tag="ACFB64_000859"
     CDS             complement(22709..23320)
                     /locus_tag="ACFB64_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC1209245.1"
                     /translation="MRRAIFLILTLTVGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAELPPLPDAHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARSSIGFGNGKLSSYKVFGYGDTVNSRWIQPRNAEWKPIGGTLGRN
                     DALRAVLYQAFCEGGVPADTQGLVQRLKERAGRYAPSMKPHDK"
     gene            23428..23652
                     /locus_tag="ACFB64_000860"
     CDS             23428..23652
                     /locus_tag="ACFB64_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC1209246.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKVLAQMRQGEVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDSSEQDGVFTLVVKHK"
     gene            23683..>24062
                     /locus_tag="ACFB64_000861"
     CDS             23683..>24062
                     /locus_tag="ACFB64_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209247.1"
                     /translation="MLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLFRSACVLTQGS
                     FEPVSKFAAVGHFVPVGKEDLSRLPPAVAEPDVQALWTLERDGGIYYLSLTRDSCSVK
                     AERADSAALLEHFSGLVRQPPKNA"
BASE COUNT         5510 a         5774 c         7122 g         5656 t
ORIGIN      
        1 tttcattccg taccatctta aggaacatca aattgggcat ttcccgcaaa atatccctta
       61 ttctgtccat actggcagtg tgcctgccga tgcatgcaca cgcctcagat ttggcaaacg
      121 atccctttat ccggcaggtt ctcgaccgtc agcatttcga acccgacggg aaataccacc
      181 tattcggcag caggggggag cttgccgagc gcagcggcca tatcggattg ggaaacatac
      241 aaagccatca gttgggcaac ctgatgattc aacaggcggc cattaaagga aatatcggct
      301 acattgtccg cttttccgat cacgggcacg aagtccattc ccccttcgac aaccatgcct
      361 cacattccga ttctgacgaa gccggtagtc ccgttgacgg attcagcctt taccgcatcc
      421 attgggacgg atacgaacac catcccgccg acggctatga cgggccacag ggcggcggct
      481 atcccgctcc caaaggcgcg agggatatat acagctacga cataaaaggc gttgcccaaa
      541 atatccgcct caacctgacc gacaaccgca gcaccggaca acggcttgcc gaccgtttcc
      601 acaatgccgg cgctatgctg acgcaaggag taggcgacgg attcaaacgc gccacccgat
      661 acagccccga gctggacaga tcgggcaatg ccgccgaagc cttcaacggc actgcagata
      721 tcgtcaaaaa catcatcggc gcggcaggag aaattgtcgg cgcaggcgat gccgtgcagg
      781 gtataagcga aggctcaaac attgctgtca tgcacggctt gggtctgctt tccaccgaaa
      841 acaagatggc gcgcatcaac gatttggcag atatggcgca actcaaagac tatgccgcag
      901 cagccatccg cgattgggca gtccaaaacc ccaatgccgc acaaggcata gaagccgtca
      961 gcaatatctt tatggcagcc atccccatca aagggattgg agctgtccgg ggaaaatacg
     1021 gcttgggcgg catcacggca catcctgtca agcggtcgca gatgggcgcg atcgcattgc
     1081 cgaaagggaa atccgccgtc agcgacaatt ttgccgatgc ggcatacgcc aaatacccgt
     1141 ccccttacca ttcccgaaat atccgttcaa acttggagca gcgttacggc aaagaaaaca
     1201 tcacctcctc aaccgtgccg ccgtcaaacg gcaaaaatgt caaactggca gaccaacgcc
     1261 acccgaagac aggcgtaccg tttgacggta aagggtttcc gaattttgag aagcacgtga
     1321 aatatgatac gaagctcgat attcaagaat tatcgggggg cggtatacct aaggctaagc
     1381 ctgtgtttga tgcgaaaccg agatgggagg ttgataggaa gcttaataaa ttgacaactc
     1441 gtgagcaggt ggagaaaaat gttcaggaaa cgagaagaag gagtcagagt agtcagttta
     1501 aagcccatgc gcaacgagaa tgggaaaata aaacagggtt agattttaat cattttatag
     1561 gtggtgatat caataagaaa ggcacagtaa caggagggca tagtctaacc cgtggtgatg
     1621 tacgggtgat acaacaaacc tcggcacctg ataaacatgg ggtttatcaa gcgacagtgg
     1681 aaattaaaaa gcctgatgga agttgggagg tgaaaacgaa aaaaggtggg aaagtgatga
     1741 ccaagcacac catgttccca aaagattggg atgaggctag aattagggct gaagttactt
     1801 cggcttggga aagtagaata atgcttaagg ataataaatg gcagggtaca agtaaatcgg
     1861 gtattaaaat agaaggattt accgaaccta atagaacagc atatcccatt tatgaatagt
     1921 aatatttatg aaaaattagg agattaatga tgaaaagaat taagtgcttt tgtgataaat
     1981 ttccatcagg agatacattt agaatgtgta tcattctgga tgactatgat aatagggttg
     2041 attattatgt aggaatatat gattacatta cgtctacctt aatgagcgat atttactatc
     2101 gatccacgat tgatgagcat ttcaagatta tagaattaat agaaaataat ccaaatgaaa
     2161 tttatgatga tggcggtggt caacaatttt gcctagaatt tcatcatgat aaggtcattt
     2221 tttaccacaa tgaatttgat gaagaagatg gttatccagt attaagctgt tcgctgcata
     2281 cttttaaaac tgctttaatt gcttggaatg cttttttgca attgcctaaa agtattcatt
     2341 cggtggtgga gactgtgatt gaggaataag cataattagc ttaatgaata gaatcagcga
     2401 tatagattgg actgcaaatc cacgcttata cgctgtgcca tgattaacag gaagagagca
     2461 acttggatgg gctagtaggg atggtaagca tttaaatata tcaattgatg gaaagattac
     2521 acacaaatga ctaatttaaa attagatttc tactctgaag ttattataaa agattcttgc
     2581 cctaatgatt tgttagaaaa tggtgagact attaaaggaa aaaaaggagt ggtattaggt
     2641 ataagcgaag aagatggtat aatctatggc tatactattt tactttttga tataaaatat
     2701 tgtatatata tagataagaa atatattata ccgactggaa aaaaattctc tcgcgatgat
     2761 ttttattagc taatttaagt aatttaagca agttttttag atactgaaac tggtaattat
     2821 gtttatgtaa gcataaggag ttcagattga aggattctac agggcaaatg aggctggcaa
     2881 ccaaggattt ggcggaagcc attaaacgag gagaagtacg tagttctgct tttacaacaa
     2941 agcaactaaa ggcaatcgaa aaaggagaat ctaaaatacc agattacact tggcatcatc
     3001 atcaagatac aggaaggatg caattgattc gtgaaggctt gcatcatgat accggccata
     3061 tcggttggag agcgatgagt aaaggaaagt aactatgtgg aaaatcataa aagaggatag
     3121 tgatgattta ggatttgcaa ttaaatgctt attctctcaa tctattgatt taaatgaatt
     3181 caagttatgg attgaacaag taatacgcga tatgcccatc gaggacatcc ctttttatat
     3241 ttttgacttg gcggatttca atgggggaat tggagacatt gacaatattg taggttttgt
     3301 ttcgagttac agcctatcaa aatcgaaaaa aaatgccttg acaggcattg ccttcttaag
     3361 ggggatagat gtctatagac cctcccgttt caaaagaaaa agcattaaaa gccttagaga
     3421 aatatcctga aatttatcat aaatttaaat ggttctttcc ttttgtagag cttccgcttc
     3481 tttaaaggac aatatgccgt ctgaaaagtt ttcagacggc atttttattt cttccagtag
     3541 gcgggggtga agaggatgaa gacggtgaag atttccagcc tgcccaagag cattgcggta
     3601 acgcagatcc atttctgcat cacgtccaaa ccggcgtaat tgccggcggg cccgacttcg
     3661 cccagtccgg ggccggcgtt ggtaatgcag gcgatgacgg cggtaaaggc ggtggtaaat
     3721 tccataccgc tcgccatcag caggaagctg aagaggacga cggtcataaa gtagatgaag
     3781 atgaaggaca taacggtcag cgcgaggcgg tcgggtatgg ctttgccgct gattttgacg
     3841 gtgcggacgg ctttggggtg cagcagcacc atcatttcgc gcaggctgaa tttgaacagg
     3901 acgagggcgc gtatggtttt gatgccgccg ccggtcgagc cggagctggc gaggatgttg
     3961 gcgaggaaaa acatccacag ggaaatcagg agcggccatt gtgcgaagtc ggtgttggac
     4021 agcccgtttg ccaatccgat ggagacgaag ttgaaggcgg tgtagcgcag ggattcgccg
     4081 aagccggcgt aatggccggt gtgccacagg tacagggcgg cggcaaggat gctgccggag
     4141 agcagcagca gcatcgtccg gcattcttcg tctttccaat aggttttgag gctgcggctg
     4201 ttgagggcgg cgaaatggct ggcaaaattg atgccgccga aaatagtgaa aacgatgatg
     4261 actgcttcga tgagggggga gttgtaataa ccgatgctgg catcgtgggt ggaaaacccg
     4321 cccagcgaga gggttgccag cgcgtgacag acggcatcga accaacccat cccggcaaaa
     4381 tgcaggcagg ctgccgcgag gatggtgatc agggtgtagc cgaaccagag ttttttcgcc
     4441 acttgggaaa tgcgcggcga cattttgctt tctttgtcaa tgccggggat ttcggctttg
     4501 aataactgcg tgccgcctac gccgagcata ggcaggatgg cgacggcaag gacgatgatg
     4561 cccatcccgc ccagccagtt gagcatatgc cgccaaaagt tgacggaggg ggcgagcccg
     4621 tcgacgtggg ggatgacggt cgcgccggtg gtggtcagtc ccgacatcga ttcaaaaaat
     4681 gcgtcggtaa agcccatatt cgggaaatac aggtacatcg gcatcgcagc catagcggca
     4741 aacgccagcc acaacatcag gacgagggtg aagccgtcgc gcgggcgcag ttcgcgcctg
     4801 aaccggaggg tggcgagccg gacgatgcac gagccggaaa gggtaacggt cgcggtggtg
     4861 gcgaatgcgg tgtacgcgcc gtccgaaaag gcgtaggaaa gggcggcggg tatcagcagg
     4921 ataaaggaaa acagcatacc cagtcgggag aggacgtggg cgatgggcag gattttgtgc
     4981 atagtggggc ggtccgttat tttgcgaagc ttttccagtc tatgccgccg gcggcttgga
     5041 cttgggtaat ttcttccgtt tcgaggttga cggcggtcag ctgcccgccc cacagcgcgc
     5101 cggtgtccag cgagatgacg ttgtcggcat tcgtgtagcc cagcgaggac cagtgtccga
     5161 agatgatggt gtggtcgagg ttttgccgat cgggggcttt gaaccacggg cgcaggtaag
     5221 gcggcatttt tttcactgtg gatttgtagt cgaaatccag ttcgttttta aaggtcaggg
     5281 cgcgcatccg cgtgaaggcg ttgacgatga agcgcaggcg ggcatagcct tccaaacctt
     5341 cgtcccatgc ggccggtttg ttgccgtaca ttttggagaa gaatttgacg tattttttgc
     5401 cgcgcagttc ggcttcggct tctccggcga gcgattcggc tttggctatg cgccattgcg
     5461 gcaggatgcc ggcgtgtatc atcacgcggc cgccctcgcg tatcaaaagc ggttgcgcgc
     5521 gcagccagtc gagcattttt ccgccgtcgg ggtgtttgag tatgggttcg attgtgtcgc
     5581 tgcgtttgag cgcgccttcg ccgcagccga cggcgagcag gtacaggtcg tggttgccga
     5641 ggacgatttg cacgctgttt tcgtgccgga tgcagaattg cagcgtttcg agggatttcg
     5701 ggccgcggtt gacgatgtcg cccgtcagcc agagggtgtc cgtgccgtgg ttgaagccga
     5761 ttttgccgag cagcgcggtc agttcgtcga aacagccttg gatgtcgccg attgcgtaat
     5821 gtgccattgc gggtgttgtg aagtgggaaa gtgttgcggt tcggacggca cggttggaaa
     5881 tcttatgccg tccgaacgtg gaattatgcg ttcaaaacga ggacggcttc cgcttcgacc
     5941 tgcacgcctt tgggcagcga ggcaacgccg acggcggcgc gggcggggaa cggctcggtg
     6001 ataaattccg ccatcacttc gttgaagacg gcaaaattgc ccaagtcggt caggtaggcg
     6061 ttgagtttga cgatgtcgtc cagcgaaccg cctgccgctt cggcgacggc ctgcaggttt
     6121 tggaacactt ggcgcgcttc ggtgtggaaa tcgccgttgc cgaccacagt catcgtggcg
     6181 ggatcgagcg gaatctgacc gctcatgtaa acggtgccgc cggcgcgcac ggcctggctg
     6241 tatgcgccga tggcggcggg ggctttgtcg gtatggatga cggttttgga catttcggat
     6301 tcctcaaaaa atagggcggc agaagccgca gcgttcggga ttatcgtaca aaaccgccgg
     6361 cttgtgtagt tttaggcggt atatgtgccg gaaacaaaaa accgccgaag gctcggcggt
     6421 ttgcggaata acgcgtatat cagaatttga cgcgcacgcc ggcggacagt tcgccggaac
     6481 ggacgttttt gacagtgttg actttgccga tgtagttgta gcggtagccg gcatccagat
     6541 cgacattggg tgtaacggca tagcttacgc ccgccaatac gccgaggccg gcggaggttt
     6601 tgctgaagct gtcgctgccg cccaagtggg cggaagcgcg gttgaggctc aagcgcgcgc
     6661 cgaaatacgg tttgacgggc gattgggtgt cgaagtcgta aatgacggac gcgccgatgc
     6721 tgtaaagttt gaaatcggtg gatggggctt gtttatagtt tttgtagcgc gtgtaatcga
     6781 cggcgaagcg gaggtcgttg atgcggtagc ctgcggagat gcgcgggctg aagcctttgg
     6841 cagaacctaa agagcttgag gctttggcgt gtgcggcatc ggcttggacg taaaagccgg
     6901 atgcgccttc cgccagtgcg gcggccggga gtgcgagggc aatcagtgcg gcaagtgctt
     6961 ttttcatatt ttggttcctt tatggtcagg gagaaaaatt gttaataatc cgttaaagaa
     7021 tcctgccgta ttatactgaa attttctttt tgcatcgtaa tattttcaat gcctcaagat
     7081 acgcggcggc atccggctgc tttgccgacg gcaaagccgt taacccgcgc gttgccctta
     7141 aacggcggca tcacgcggcg gatgggtgaa acttgcaaac ggtttggaaa aaaccgcaaa
     7201 ggcggggcgg cggcatttgt cagattgttg caggcgcagg cgtacggttt tttgtgtgcg
     7261 gcgttacctt aggcgtcgga catttccggc ggcggctgtg ccgtctgaaa cgcccggcgg
     7321 gggatgcggc tgcgtttccc atcgataggc atatttgccg gccgcgttcg gggcgggttt
     7381 tacccgggcg gccgccgatt tgtttgcgct tataaaaaac actgcaacaa atcgtttaaa
     7441 aacaagcgtc ctttttcggt cgggcggaat acggtcgggt cggtttccag caggcctttt
     7501 tgccttgccg tttcgatttg caccatgatt ttggcggcgg gtacgcccgt gcgctcctgc
     7561 aacatcgcgg cgggtacgcc gtcggtcagg cgcagggcgt tcatcatgaa ttcgaacggc
     7621 aaatcttcgg cggcgacggt tttgcgttcg acggcttcgc ccggctgact ttgcattaag
     7681 gcgaggtagt cgttggggtg gcggcggcgg acggtgcgct cgatgcggtc gggataggag
     7741 attttgccgt gcgcgcccgc gcctatgccc aaataatcgc cgaactgcca gtagttcaaa
     7801 ttgtggcggc actgcatggt gggtttcgca aaagccgatg tttcgtagtg gataaaaccc
     7861 gcgccttcca gtgtgccgtg taccgcgtct tcgatgtcga gcgccgcttc gtcttgcggc
     7921 aaacctttcg gcggcgtatg accgaacggc gtgttcggct ccatcgtcag atgatatgcg
     7981 ctgatgtggg ttgcgtctgt ggcgatggcg gtttgcacgt cgtccaatgc cgtctgaacg
     8041 gtttggttcg gcagggagta catcaagtcg atattgactt tatcaaataa ttttaaggcg
     8101 gtatcgatag cggttaaggc ttctttgcca ttgtggacgc gccccagcct tgagagcata
     8161 tcgtcgttga aactctgtac gccgatagaa agccgcgtga tgcctgcgtc tttaaatcct
     8221 tgaaacttct cgatttcaaa tgtacccgga ttggcttcca acgtaatttc cgcttcgggc
     8281 tgcaagcgca acagcgaacg cacgccgctt aacaaacggt caatcgattc cgcctgaaac
     8341 aggctgggcg taccgccgcc gaaaaatatc gtttccaccg gcctgcccca aatattgggc
     8401 aattcaagct gcaagtcggt cagcagcgcg tcgatatagg cggcttcggg caatccgttt
     8461 ttcaggctgt gggaattgaa gtcgcaatac gggcattttt tgatgcacca cgggatgtgg
     8521 atatagagcg acaggggcgg cagggcggtg agtcgggtgc ggtttggaaa ggaaatggtg
     8581 tgcatggtgc ggttcggaaa agtggacgat gccgtctgaa ggcggttcgg acggcatggg
     8641 ttcagcagag cagggtaagc agttcggctt cgctgaggac ggaaacgccc aaggcattgg
     8701 ctttttccag cttgctgccc gcggcttctc cggcgacgac gtagtcggtt tttttggaca
     8761 cgctgccgga cactttgccg cctgcggctt cgattagggc ttgggcttgg tcgcgtttga
     8821 aggtgggcag ggtgccggtt aacacaaagg ttttgcctgc cacggcttta ttgatgccgt
     8881 ctgaaccttg cgccgcctcg tcttcagacg gcatttgcgc gaagaaggtt tttaggtttt
     8941 cgagcagggc ggtgttttgc gctttgctgc gccacgcctg ccagtcggca gggagggctt
     9001 tgtcgttttg cagtccttct atactttgtc cggcgagttc ccataaggct tgggctttgt
     9061 tttcgctgat tttgaaaccg ggcaggcggg taatccagcg ttgcggcccg gcatattgtg
     9121 cggcaggcag gctgacggct tgggcttgcg gggcaacgcc tgcggcgagc agctcgtcta
     9181 tcatcgcctg ctgttcggct tgggcgaaga agtgggcaat ggaacgcgcc actaccgtgc
     9241 cgatgtcggg caggcaggcg aggacgggtt cgggggcgcg gcggacgcgt tccaatgtgc
     9301 cgaatgcctg cgccagcgtt ttggcggtgc gttcgccgac gtggcggatg ccgagcgcga
     9361 acaggaagcg ggcaagttcg ggcgttttgc cggattctat gcctgcgagg atgttttgcg
     9421 cccacttggt cggttgtttt ttgccgtttt gggtattgta tttagacagg tcgcctgaaa
     9481 ccgtttctgc gtcgccgttt tcgttttcag atgacccttt atccgccgtt tccttcattt
     9541 tttgcagggt cggaatatcg atgcggtaga ggtcggcgaa atggcggacg aggtcttggg
     9601 cgaccagctg ttcgatttgt ttttcaccca agccgtcgat gtccatcgct ttgcgcgagg
     9661 cgaagtggat taagccttgc gcgcgttgtg cctgacaaag cataccgccg ctgcatcggg
     9721 cgacggcttc gccttcttcg cgttcgattt cgctgcggca gatggggcag cgggccggca
     9781 ggcggtaggg cttatggagc ggaacggatt cggtttgctt ggcggacggt gtttcggcaa
     9841 acaggtcgtc ctgctgatgc ccgatgccgt ctgaaacggc aacggcggtt tcctgcatcg
     9901 ggcggcgttc gaaaatcacg cgcacgactt cgggaatcac gtctccggcg cggcgcacga
     9961 cgacggtatc gccgacgcgc acgtctttgc gcgatacttc gtcctgattg tgcagggtgg
    10021 cgttggtaac ggttacgccg ccgacgaata cgggttgcag gcgggcaacc ggcgttaccg
    10081 cgcccgtcct gccgatttgc acgtcaatcg cttcgacaat ggtcagggct tcttcggcag
    10141 ggaatttgtg ggcaaccgcc caacgcggcg cgcgggagat gaagccgagt tcgcgctgtt
    10201 gcgccaagct gttgactttg accaccatgc cgtcgatttc gtagggcagt tcggggcgtt
    10261 tttgctgcat gtgttcgtaa aacgccaata cttcgccgat atttttgaaa cagccgaaat
    10321 tgccattggg cagactgaag ccgagttctt ggaaataggc gagttcttgg atgtgttctt
    10381 cagcttcgaa accgccttgc tgacgggcaa tcgaataggg gaaaaagtgc agtttgcgtt
    10441 gcgcggtgat gcgcgaatcg agttggcgca ggctgccggc ggcggcgttg cgcggattgg
    10501 caaagggttt ttgcccgttt tcggcttggc gtttattgag cccggcaaag tcggctttga
    10561 gcatcagcac ttcgccgcgt acctcgatga gttcgggcac gttttcgccg tgcagccgca
    10621 gggggatgtt ggatacggtt ttgacgtttc gggtaacgtc ttcgcccgtc gtgccgtcgc
    10681 cgcgcgttgc cgcctgcacc aatacgccgt cgcggtagag caggctgatg gcgaggccgt
    10741 cgaatttggg ttcgataacg tattcgggat tgccgccgtc caagccgtcg cgcacgcgtt
    10801 ggtcgaaggc gtacatttcg gcatggtcga acacgccgtt ttcatcttgc ggggaaaagg
    10861 cgttggtcag cgacagcatc ggcacttcgt ggcgcacttc ggcaaagcct gccaaaggct
    10921 cgccgccgac gcgctgggtc gggctgtcgg gcagcttgag ctcgggatgg tttcgttcca
    10981 acgcttcgag ttcgcggaac aggcggtcgt attcggcatc gggtatgctg ggcgcgtcga
    11041 gggtgtagta ttcgtaggcg tagcggttga ggaggtcggt gagttcgtgt atgcgttgtg
    11101 cggtcgggtt catggattcg tacaggttca gacggcatgg acaaatgccg tctgaacggt
    11161 aaaacgtgtt ggaaatggtc ggattttacc tgaaaacaaa aggcggatgc accgttgccg
    11221 atgtatccgc ctttgtgttt gcggagttat gaaaacaggc gcagggcggt tttgccgccc
    11281 ggttcgatac cgactttgag catctcggac tgacgcgcca atacataagt gcgtacgtct
    11341 ttgagccatt gggtcgaaac ttcttccatt ttgtcgttga ccagattcag gttcaactga
    11401 ccggacaggc gtaccgccaa atccataaac aaatcgtcga aggttttttc gcctgccgga
    11461 gagtgcggga tgtcgagcag catactgaag cctttgtagg actggttgtc caaaagggca
    11521 ttggtaaacg gctcgttgtt gagcgagcag atggagaaca tggtcgagcc cgacgtgtcg
    11581 gtatagtgga acgcgccgtc gtcttccaaa acgaaaccca cgcccgttac ggcggaacgc
    11641 agttctacgc cgctgatgct ggtcggcgaa accaaatgga tggcgatggt ctggtcgacg
    11701 cgcgcgcaga atgcgtccag tgcggaagcc acttcgataa aggcggcaag gtcggtgtgc
    11761 agcgtctgac cgcccatgct ttgtgcgaat gcgtccgcct ggcggttgaa tgcggagagt
    11821 tcttcctgcg aggcaagtcc gttgcggctg actgcctgga tacccacgat aaatgcctga
    11881 tagcggatgc ccgggatggg ttcggcaatc tggaaatggt cgtccatggt gcagccgaca
    11941 atctggtagc ggcagcggtt ggaaaggcgc ggcagtgcgt gcagttcttt ggcttcggtc
    12001 agcgcgatat aggagatgaa gtcgaagcgc atgtcaaacc agggcagctc gaccttcgac
    12061 agctctttga gcgtaatcag cggttttgcc ggcgtttgcg gaacgggaac gggttttgcc
    12121 ggcgcgtcgg caggtttcgg tgcggaatgt ccggtttggg gttcggaaac ggtgtgggcg
    12181 gagttgccga taatgccgat ttcttccaag gcggtttcga tttcggtttt gaacggggag
    12241 gcttttgcct gtttctgctt ggcgatgtag acggcatcct gttcttgcag gttgcgcatg
    12301 gcggagtctt ggggtttggc cggttttttg accgccggtt ggggtttcgg catcatgact
    12361 ggcccgccgg acggtttgcc gtcgcggaca tggctggttt tgctgttgag cagggcatct
    12421 ttgtcggagt gtccgaactg gtcgcgcact tttttgcggt attggttttc ctgatacata
    12481 ttgtaggcga caacggcgag gacggcggcg aggaacagta cgatgtaaat catggcaatc
    12541 actttaaatt tcgggatgca ggatgcgcaa agtgcgggta ctgcggttaa atcgggcttg
    12601 cgtttccggc agtctgacgg aacggccggt tataacgttt gaattataac gaaaattgca
    12661 gggtctgaca gcagtgtgtc gaaataagcg gaaattttcc gaaatgccgt ctgaaatctg
    12721 tggttttcag acggcatttc tgtccacggg aaaccctttt tcccgtatcc gccgccagtc
    12781 gaaaaaatgg ccggggtcgg ttttgcggcc gggtgcgatg tcttggtgtc ccgttaccgc
    12841 cgtgatgggg tagcggcggc agagggctgc caacagcgtt tcgagcgaac ggtattgcgc
    12901 ttcggcaaac ggttcgaaat cgcagccttc cagttcgatg ccgatggaaa acgcgttgca
    12961 cttctcccgc ccccgaaacg aggatgcgcc ggcgtggtac gccatgtcgc cgcatgatac
    13021 gaactgcacc gtttcgccgt cgcgtgcaat caggaaatgg ctggacacgt gcaaagtgtg
    13081 tatcaggctg aagaacggat gtccgtcggg gtcgagccgg ttggcaaaca gcttttccac
    13141 cgcatccgtg ccgtattcga acggcggcag cgaaatgttg tgcagcacga tcagggaaac
    13201 cgtttctccc gtttccctcg ggctgaaatt gggcgacggg gtatggcgta tgctttgaag
    13261 ccagccgttt tgccagtgtg ctccggcgtg attgtccatg atgttcttcc tgtccggcgg
    13321 gcaatttggg ttatactgtc gcccgaattt taagacgtat tccgaatgct gggaatccta
    13381 ccatgttgaa aaaaatgttg aaatggactg ccgttttttt gaccgtatcg gcagccgttt
    13441 tcgccgcgct gctttttgtc cctaaagaca acggcagggc ataccgaatc aagattgcca
    13501 aaaatcaggg tatttcgtcg gtcggcagga aacttgccga agaccgcatc gtgttcagca
    13561 ggcatgtttt gacggcggcg gcctacgttt tgggtgtgca caacaggctg catacgggga
    13621 cgtacagatt gccttcggaa gtgtctgctt gggatatctt gcagaaaatg cgcggcggca
    13681 ggccggattc cgttaccgtg cagattatcg aaggttcgcg tttttcgcat atgaggaaag
    13741 tcatcgacgc aacgcccgac atcggacacg acaccaaagg ctggagcaat gaaaaactga
    13801 tggcggaagt tgcgcccgat gccttcagcg gcaatcctga agggcagttt tttcccgaca
    13861 gccacgaaat cgatgcgggc ggcagcgatt tgcagattta ccaaaccgcc tacaaggcga
    13921 tgcaacgccg cctgaacgag gcatgggcag gcaggcagga cgggctgcct tataaaaacc
    13981 cttatgaaat gctgattatg gcgagcctga tcgaaaagga aacggggcat gaggccgacc
    14041 gcgaccatgt cgcttccgtc ttcgtcaacc gcctgaaaat cggtatgcgc ctgcaaaccg
    14101 acccgtccgt gatttacggc atgggtgcgg catacaaggg caaaatccgt aaagccgacc
    14161 tgcgccgcga cacgccgtac aacacctata cgcgcggcgg cctgccgcca accccgattg
    14221 cgctgcccgg caaggcggca ctcgatgccg ccgcccaccc gtccggcgaa aaatacctgt
    14281 atttcgtgtc caaaatggac ggcacgggct tgagccagtt cagccatgat ttgaccgaac
    14341 acaacgccgc cgtccgcaaa tatattttga aaaaataaac catgccgtct gaaaagtttg
    14401 tgttttcaga cggcataccc ttaccggaac tgcaagcatg aaaccgcaat tcatcacttt
    14461 ggacggcata gacggtgccg gaaaatccac caaccttgcc gtcatcaagg catggtttga
    14521 acggaggggg ctgcccgtgc tgttcacgcg cgagccgggc ggaacgccgg tcggtgaggc
    14581 cttgcgcgaa atcctgctca accctgaaac caaagccggt ttgcgtgcgg aaacactgat
    14641 gatgttcgcc gcgcgtatgc agcacatcga ggaagtcatc ctgcccgcgc tttcagacgg
    14701 catccatgtc gtgtccgacc gttttaccga tgcgaccttc gcctatcagg gcggcgggcg
    14761 ggggatgccg tctgaagaca ttgaaatttt ggaacattgg gtgcagggcg gtttgcgccc
    14821 cgatttgacc ctgctgttgg atgtgccgct ggaagtatcg atggcgcgta tcggacaggc
    14881 gcgcgagaaa gaccggttcg agcaggagca ggcggatttc tttatgcgtg tgcgcggcgt
    14941 ttatctcgac cgagccgccg cctgtcccga acggtacgcc gttatcgaca gtaaccgcag
    15001 cttggatgaa gtcagaaaca gcatagaaaa agtgttggac ggacatttcg gctgttgatg
    15061 cggcaaatat tgaaacaagc gcatccgccc gcgccgaaaa ccaaacggca gtgccgcagg
    15121 tgaaaatggc ggtatgcgcc aaactttcgg catgatagaa ttacgctcgg ttacaaggca
    15181 ggatgcgtcg gcaatattaa cgaaccgccc gtaacatgat gacccgaaag cgtttcggac
    15241 agttcgattc aaatcttttt ctcgcaacag gattgacaca tggaaaactc attgaaagaa
    15301 gccgccctca agttccacga attacccgtt ccgggcaaaa tttccgttac cccgaccaaa
    15361 tctctggcga ccgacaaaga tttggcgttg gcgtactctc cgggcgtagc cgccccctgt
    15421 atggaaatcc atgccgatcc gcaaaatgcc tacaaataca ccgccaaagg caacttggtc
    15481 gccgtcattt ccaacggtac ggccgttttg ggcttgggcg acatcggcgc gctggcgggc
    15541 aaacccgtga tggaaggcaa aggcgtattg ttcaaaaaat tcgccggtgt ggacgtgttc
    15601 gacatcgaaa tcgacgaaaa agacccgcaa aaactggtgg acatcatcgc cgctttggag
    15661 ccgaccttcg gcggcatcaa cctcgaagac atcaaagcac ccgagtgttt ctacatcgaa
    15721 cgcgaattac gcaaacgctg caaaatcccc gtatttcatg atgaccagca cggcacggcc
    15781 atcattaccg ccgccgccgt attgaacgcc ctgcgttata ccggccgtaa aatcgaagaa
    15841 gcgactttgg tgtgctccgg cgcaggtgcc gccgcgattg cctgcctgaa ccaactgctg
    15901 gatttgggct tgaaacgtga aaacgtaacc gtttgcgact ccaaaggcgt gatttaccaa
    15961 acccgcgaag acaaagaccg catggacgaa tccaaaaaat tctacgccgt tgaagacaac
    16021 ggccggcgcg tacttgccga tgcggttaaa ggcaaagaca tcttcttggg cctctccggc
    16081 gcaaacctgc tgacgcctga aatgttgaac acgatgaacg aaaaacccat cgtgttcgcc
    16141 atggccaacc cgaatccgga aatcctgccg ccgctggcga aagaaacccg tccggacgtg
    16201 gttatcggta ccggccgctc cgacttcccg aaccaagtga acaacgtatt gtgcttcccg
    16261 ttcatcttcc ggggcgcgtt ggatgtcggc gcaaccacca tcaacgaaga aatgaaacgc
    16321 gcctgcgtgt atgctttggc agatttggcg atggaagaag taaccgaaga agtggttgcc
    16381 gcttacggta agaaattcga attcggcgcg gaatacctga ttcctactcc gttcgattcc
    16441 cgcctgctgc ctcgcgtcgc tacggctgcc gccaaagcag cgatggaaag cggcgtggca
    16501 acccgtccga ttgcagattt ggaagcttac gctgccaagt tgggcgaatg gaagctgtaa
    16561 gccgtttgtg gttaaaatgc cgtctgaact gttttcaggc ggcattttgt tgtcagattg
    16621 ataaatgaaa gatactggaa aatgaaagag atgaaacctg tccgttatca tattggcgat
    16681 atacccgaaa cttcaaaaca aaccgccccc ggtcatgacg acagggcagt gggtgttgac
    16741 gatgattgtt ttcatgattc ctttggtcaa tatcgttatg ttttttgttt gggcgttcgg
    16801 cagaggcaac cccaaccgtg ccaatttctg taaagcactg ttcttattta ccttgttggt
    16861 tcgcttatcg gtttgatttt catattgctt ataggtgggt ctgcatcggg tacgcattat
    16921 taatgtgccg gctgattctg cttcgaagat ttgtatcgaa tatgccaaat ttttttaaat
    16981 ttcataccgt tatcgaacgg cattggcaaa aaccttatcc ggttttgtct tttctgctca
    17041 agccgctttc cgggctgttt gccaaaattg cggcaaaatg gcgggcggat tttttatcgg
    17101 gaaaacggca aagcgaaaag ctgtccgtgc ctgtggtcgt ggtcggcaac atccacgcgg
    17161 gcgggacggg caaaacgccg attgccgccg cgctggtgtc gggcttgcag gaaaaaggtg
    17221 tcaaggtcgg catcatcagc cgaggctacg ggcgcaagag caaggcggtt tatgtattga
    17281 atgccgcgag ccgtgcggaa gatgcgggcg acgagccttt gctgctgttc cgtaaaacag
    17341 gtgcgccgac ggcagtgggc agcagccgtg tggaggcagg cagggcgttg ctggcagcgc
    17401 atccggagct tgaattgatt gtggcggacg acggtttgca gcattacgcc ctgcaacgcg
    17461 atgtggaaat cgcggtgttt ccggcggcgg atacggggcg tacggatttg gacttgctgc
    17521 ccaacggcaa tttgcgcgaa cctttgtcga gattggaaag cgtggatgcg gtggtggtcg
    17581 gcggcagggc ggcggatggt tttatgccgt ctgaacattt gttcggcagc cgtattgagg
    17641 cgggtgcggt ttaccgtttg aaccgtccgt ctgaaaaact ggatatttcg acattgtcag
    17701 ggaagcgcgt cgcagcggtt gccggtatcg ccaggccgca gcgtttcttc gatactctga
    17761 cacacatggg catccgcctt gaccaaacgg ttgctctgcc cgaccatgcc gatattttca
    17821 atcgggattt gccgcctgcc gatgtggtgc tggtaactga aaaagatgcg gtcaaatttt
    17881 cagacggcat ttgcaccgat aatgtttggg tgctgccggt ttgtgcgata atcgaacctg
    17941 atttggcgga gtttgtgctg gagcggttgg agggtgtacc gaaggccgtc tgaaagcgcg
    18001 gtttggacga agtgataacg gactggaata agaacgccct acgccgtcat tcccgcgcag
    18061 gcgggaatct aagtctcgaa ttttcaggaa tgcctaggag acttcagaaa tcccaaatct
    18121 ccggatttcc acttgggcag gaatgagaaa gccggttgta ttttttatct gcattaatca
    18181 ttcgttaaag gagtggatat gaagctgaaa accttgttat tgcccttcgc cgcactggca
    18241 ttgtgtgcca acgcatttgc cgccccgccc ggcgacgcgt cgttggcacg ttggctggat
    18301 acgcagaatt tcgaccggga tatagaaaaa aatatgattg aaggctttaa tgccggattt
    18361 aaaccgtatg cggacaaagc ccttgccgaa atgccggaag cgaaaaaaga tcaggcggca
    18421 gaagccttta atcgttatcg tgagaatgtt ttgaaagatt tgattacgcc cgaagtgaaa
    18481 caggctgtcc gcaatacctt attgaagaat gcccgtgaaa tatacacgca agaagaaatt
    18541 gacggcatga ttgcctttta cggttcgcct gtcggtcagt ccgtggttgc caaaaatccg
    18601 cgcttaatca agaaatcgat gagtgaaata gcggtatctt ggactgcatt gtcagggaaa
    18661 atcgcgcgac atcatctgcc cgagtttacg gaagagttac ggcgcatcat ctgcggcggt
    18721 atagtggatt aaatttaaat caggacaagg cggcgaagcc gcagaccgta caaatagtac
    18781 ggcaaggcga ggcaacgccg taccggttta aatttaatcc actataaaaa tcccgatgcg
    18841 ggctgcaagc aggccggaca gcttgggaaa aggtatcaga agtaaataat agccgcctga
    18901 aatattgcgg agggcatccg attgattaaa ccatcaaacc cgaaagcaac cttatggaaa
    18961 aaaaattctt agacatcctc gtctgccccg ttaccaaagg caggctggaa tatcatcagg
    19021 acaaacagga attgtggagc cgtcaggcga agcttgccta tccgattaaa gacggcattc
    19081 cctatatgct ggaaaacgaa gcacgagcgt tgagcgaaga ggaactcaaa gcatgaccga
    19141 attcgtcgta ttgattccgg cgcggctgga ttcgtcgcgc ctgcccggaa aagccttggc
    19201 ggacattcac ggcaaaccga tggtcgtgcg cgttgccgaa caggcggcaa aaagcaaagc
    19261 cgcgcgcgtc gtcgtcgcca ccgatcatcc cgacattcag acggcctgtc aggcgcacgg
    19321 tatcgaagtc gtcatgactt caaaccggca cgaaagcggc acgacgcgcc ttgccgaagc
    19381 cgccgccgcg ctgaagctgc cgccgcattt gattgttgtg aacgtacagg gcgacgagcc
    19441 gctgattgcc cccgaactca tcgaccgcac cgccgaagta ctcgtcgaaa acaacgtcca
    19501 aatggcgacc gccggccacg aattgcacga tttcgacgaa ttgatgaatc ccaatgccgt
    19561 caaagtcgtc ctcgacaaaa acggcaacgc catctacttc agccgtgccc cgattcccta
    19621 tccgcgcgat gcgatgcgtg ccggaaaacg cgaaatgccg tctgaaaccg ccgtcctacg
    19681 ccatatcggc atttacgcct accgcgtcgg cttcctgcaa cgctatgccg aaatgagcgt
    19741 ctcgccgctg gaaaccatcg aatcgctgga acaactgcgc gtcctgtggc acggctaccc
    19801 catcgccgtc gaaaccgcca aagaagcccc cgccgccggt gtggatacgc aggaagattt
    19861 ggacagggtt cgcgctgtat ttcagaccgt ataaaacagg ttcaaaggga aaagatatgc
    19921 agcaacatat tgaaaagtgg caacacttga gccgtgaaga acagaaaatc cttgccgaag
    19981 tatggggtct cgtgcaaaac gatgatcagg aggttcacta tgaaatgctc aaattgaacg
    20041 cacctgatga agtcagcggt gaattttggt tcagaatggc agaaacactc agcaccctgc
    20101 cgcccaatcg ttccctcgac cttagaatga acggcggcag gctgtcgacc gccgtatcca
    20161 tcctttccgt catgattgaa gacaatcccg acataccgca gctttgggcg taaaaaatta
    20221 ccgccctcaa ttatttggta cacggacaca aagcccgtgc cgacggtttg gtacaacagc
    20281 ccgacaaagc ggcagaagcc aacgaggagg aatacctgac caaagccctg tcgcaaaacc
    20341 tgctgtcaac attggatgcc gcacttgcac gttttcctga agacgcgtgg tttcaggaaa
    20401 tcaaacagga tgcacaaaag cattttgctt gaggacgtgg cggtcaggaa tatttccatt
    20461 caggaagaaa agaagtgcct aattgggtat aatcagggta aatcttattt tatttcaaaa
    20521 gattaatatt tgttttccgt ttttccttga cggtatcgga aaagttgatt atagttacag
    20581 cttccttagg agtaatggct gagaggctga aggcacttcc ctgctaagga agcatgtggg
    20641 atcaacctgc atcgagggtt cgaatccctc ttactccgcc agataaaaaa atagacgctg
    20701 tgttttacag cgtctatttt tttatgcaat tttatagcgg gttggcgcaa acccggtatg
    20761 gtattgccct gtcttgattc tgaattttgt taatccgaga tgtttgccgt ttatttttgc
    20821 ctcgttcaaa cggcggctct gatctgcgcg gtttctgttt gccgtattcg cctacccgta
    20881 ccgcaaatgt tatactggga aaaattcacc gattgtattt tacggcgtat ttgccgatag
    20941 gatggaagag acaaatgagc agaatccggc aggcttttgc cgctttggat ggcggaaagg
    21001 cattgattcc ctatattgcg gtgggcgatc ccgatattcg gacaactttg gcattgatgc
    21061 acggcatggt tgcaagcggt gcggatattt tggagttggg cgtgccgttt tccgatccga
    21121 tggcggacgg gccggttatt cagcgtgcgg cggagcgggc gttggcaaac gggatttcgc
    21181 tgcgcgatgt cttggatgtc gtcagaaaat tccgtgaaac cgacacgcaa acgccggttg
    21241 ttttgatggg atatttgaat cccatacata agatgggtta tcgggagttt gctcaggaag
    21301 ccgcaaaggc gggggtggac ggcgtgttga cggtggattc ccccatcgaa accatcgatt
    21361 ctttatatcg cgagctgaag gataacgagg tcgactgtat tttcctgatt gcgccgacga
    21421 cgacggaaga ccgtattaaa accattgccg agctggcagg cggatttgtc tattatgttt
    21481 cgctcaaggg cgtaacgggc gcggcaagtt tggatacgga tgaggtttcg cgtaaaatag
    21541 agtatttgcg tcagtatatc gatattccca tcggtgtcgg tttcggcatc agcaatgcgg
    21601 aaagcgcgcg caaaatcggc cgggttgccg ccgcgatcat tgtcggcagc cggattgtga
    21661 aagagattga aaacaatgcg ggcaacgagg ctgccgccgt cggtgctttg gtcaaagagt
    21721 taaaggatgc cgtgcgctga ttctgtcgcg cattctaaat gttttaggag ttgtccatga
    21781 gctggttaga taaaatcctg ccacccaaaa tcaagaatcg tgggaaagac ggttcttcca
    21841 atgttcccga gggtttgtgg cgcaaatgcc cgtcttgttc ggcaaccgtt tattcgaccg
    21901 agttgcagca gaacaatcag gtctgcccga aatgcaacca ccacaatccg ttatcggcgc
    21961 gccagcgttt gaacctgctt ttggatgagg aaggcaggga ggaagttgcg ggtaatgtca
    22021 aaccgaccga tcctttgaag tttaaagaca gcaaaaaata tccggaccgt ttgagtgcgg
    22081 cacgcaagct gaccggggaa gatgacgcgc tggtggtgat gaaggggatg atgaacggtc
    22141 tgcccgtcgt cgttgccgcg tttgaattcc gctttatcgg cggttcgatg ggttcggttg
    22201 tgggcgagcg tttcgtacag ggtgtccgcc gtgcggttgc cgataattgt ccgtttgtct
    22261 gtgtggcggc ttccggcggc gcgcgtatgc aggagggtgt aaactcgctg atgcagatga
    22321 cgaaaaccag tgccgcgctg catttgctga cggaaaaacg cctgccgttt atatcggtgt
    22381 tgaccgatcc gactatgggc ggcgtatctg ccagcttcgc atttttaggc gatgtcgtgc
    22441 ttgccgaacc gaacgcgctg atcggttttg ccggtccgcg cgtgattgag cagacggtgc
    22501 gcgaaacgct gccggaaggc ttccaacgcg ccgagtttct gctggaaaaa ggcgcgatcg
    22561 accagattgt cgaccgccgc gatatgaagc ggcgcatcag tgatttgatt acgctgttgt
    22621 gccgtcagga caaagtttcc gccacctgat ggctgatgaa tcgaatgccg tctgaaaccg
    22681 atgtttcaga cggcattttt gtgtctggtt atttgtcgtg cggcttcatc gacggggcat
    22741 agcgtccggc acgttctttc aggcgttgta ccaaaccttg cgtgtcggcg ggtacgccgc
    22801 cctcgcagaa tgcctgatac aggacggcgc gcaatgcgtc gttgcgccca agtgtgccgc
    22861 ctatcggttt ccattcggcg tttcggggct gtatccagcg gctgttgacc gtatcgccgt
    22921 atccgaatac tttgtaggag gaaagtttgc cgttgccgaa cccgatggag gagcgggcgc
    22981 agaatatgcc ttcggcagtc aggttgtcgt agcctttgtc ggagcggata ttgagaatgt
    23041 agcggatgct gccgtcgggc gcgggcataa tttgcaggct gtcgagcagg attttcggct
    23101 gtttgccgta attttcatcc acataaatgt caaaccagcc gtccgagtgc gcatcgggca
    23161 gaggcggcag ttcggcggta tgttctttaa attcgcgggc ggcggcttct tcgggcgttt
    23221 cgcggtagcg ggtgttgatc ggcgtgtctt tttggctgaa gccggcggca agggacgtgc
    23281 cgacagtcag ggtcagaatc agaaagatgg cgcggcgcat aagtttctcc aaattgaaaa
    23341 cggctttatt ttatggtttg gcgggaaggg ctgcaagcaa tcggggtata atctgacctg
    23401 attttcattt taaagcggtg tcgaaccatg aacagcgaaa ctttagacgt aaccggattg
    23461 aaatgtcccc tgccgatttt gcgggcaaaa aaggttttgg cgcaaatgcg gcagggcgag
    23521 gtattgaccg ttctggcgac cgacggcggc gcgccggggg actttgaggc tttttgccgc
    23581 caaaccggtc atgtgctgtt ggattcttcc gaacaggacg gcgtgttcac actggtcgtc
    23641 aagcacaaat aaatgccgtc tgaaatgcgg atgtcctgat tgatgttatt gctgtcggca
    23701 tttgtgcttg ctgcgtgtaa tgcccaaaac cggcaatccg gtgtacggca tcaggatttt
    23761 gcacgcgtag ccgtcgattt gtttcggagt gcctgcgtcc tgactcaagg tagtttcgaa
    23821 ccggtttcca agtttgccgc tgtcggacat tttgtccctg tcggaaagga agacctgtcc
    23881 cgtctgccgc ccgctgttgc agaacccgat gtacaggcgt tgtggacgct tgaacgcgat
    23941 ggcggcattt attatttgag cctgacgcgc gacagttgca gtgtgaaagc cgagcgggca
    24001 gacagtgccg cgcttttgga acatttctca gggcttgtcc gacagccgcc gaaaaatgcc
    24061 aa
//
LOCUS       DAXDXW010000024        23413 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXDXW010000024 DAXDXW010000000
VERSION     DAXDXW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23413
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.024"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(2..466)
                     /locus_tag="ACFB64_000862"
     CDS             complement(2..466)
                     /locus_tag="ACFB64_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209248.1"
                     /translation="MKRFILPVLLSATTALASPIVGTWHCIGTDENIHSDTKVKYLQD
                     GSFRGDAILKIDDDGNILAYRVVGEGKWRFANNALTQSQIKYGEVSRQHSPETLAWLE
                     KSEDARLLESMMYTGLVAQMDKPGKDDVYQLDKSGKLVSEDGTSREACTKVE"
     gene            complement(543..3380)
                     /locus_tag="ACFB64_000863"
     CDS             complement(543..3380)
                     /locus_tag="ACFB64_000863"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC1209249.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFRPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDGVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESMEEQGSMWHIRYPLADNPTEAVIVATTRP
                     ETLLGDAAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQPGFSLPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKPHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDNEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKAVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLMKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGHSFPDMWIIGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPETDGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGETGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEARQVAAL
                     PESGDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(3485..4744)
                     /locus_tag="ACFB64_000864"
     CDS             complement(3485..4744)
                     /locus_tag="ACFB64_000864"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC1209250.1"
                     /translation="MKVLVLGAGIAGVSSVWYLAEAGHEVTVIDRTEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKRLFKSHPPLLFRPDGGLYQIEWLWRMLQNCTATRYQ
                     INKERMVRISEYSREMFRRFEAQTDMNFEGRKKGTLQIFRQTEEVEAAKQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIVGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     YFNQTISRIDHNGLRIKAVETETGRFETDAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYETKLPEKRRETLAL
                     VVNDLFPEGGDLSQALSWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSPGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQKL"
     gene            complement(4823..6214)
                     /locus_tag="ACFB64_000865"
     CDS             complement(4823..6214)
                     /locus_tag="ACFB64_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC1209251.1"
                     /translation="MQVFLDNLKAFFETVSSWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGTGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIVINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPELSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAALYRVGYVSSVMPGTVLSLDLVWLASDTFNGLMALPNLTALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            6420..6560
                     /locus_tag="ACFB64_000866"
     CDS             6420..6560
                     /locus_tag="ACFB64_000866"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209252.1"
                     /translation="MWRAAEIINAADCHTFEIYSGLNLNRYGVASPCRTSCTVCGFVA
                     LS"
     gene            complement(6677..7453)
                     /gene="lpxA"
                     /locus_tag="ACFB64_000867"
     CDS             complement(6677..7453)
                     /gene="lpxA"
                     /locus_tag="ACFB64_000867"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC1209253.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVRIGANTEIGPHA
                     VINGHTTIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFQDFFAQSTRGIIR"
     gene            complement(7496..8176)
                     /locus_tag="ACFB64_000868"
     CDS             complement(7496..8176)
                     /locus_tag="ACFB64_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFC1209254.1"
                     /translation="MERYKNAVRKDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CFRQEFKDRNRLYDSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDAAGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINNFRNRLAHHEPICFKGAIKDTGYARNIYQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(8383..8832)
                     /gene="fabZ"
                     /locus_tag="ACFB64_000869"
     CDS             complement(8383..8832)
                     /gene="fabZ"
                     /locus_tag="ACFB64_000869"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC1209255.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(8868..9911)
                     /gene="lpxD"
                     /locus_tag="ACFB64_000870"
     CDS             complement(8868..9911)
                     /gene="lpxD"
                     /locus_tag="ACFB64_000870"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC1209256.1"
                     /translation="MIPATCTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAIIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRHVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHIAGIFPMSEHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDSKDTQ"
     gene            complement(9944..10444)
                     /locus_tag="ACFB64_000871"
     CDS             complement(9944..10444)
                     /locus_tag="ACFB64_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC1209257.1"
                     /translation="MTRLTRAFAATLIGLCCTTGTHADTFQKIGFINTERIYLESKQA
                     RNIQKTLDGEFSARQDELQKLQREGLDLERQLAGGKLKDAKKAQAEEKWRGLVEAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQDVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(10510..12888)
                     /gene="bamA"
                     /locus_tag="ACFB64_000872"
     CDS             complement(10510..12888)
                     /gene="bamA"
                     /locus_tag="ACFB64_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC1209258.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSDRFDRQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTRQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAGTKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDIYGKAFDPRKASTSVKQYKT
                     TTAGGGVRMGIPVTEYDRVNFGLAAEHLTVNTYNKAPKRYADFIKQYGKTDGADGSFK
                     GLLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGRTYTAAENGNNKSVYSENAH
                     KSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(12945..14285)
                     /gene="rseP"
                     /locus_tag="ACFB64_000873"
     CDS             complement(12945..14285)
                     /gene="rseP"
                     /locus_tag="ACFB64_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC1209259.1"
                     /translation="MQTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARTGFQSGDKIQSVNGVSVQDWSSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTVEWIRGKPLGERVQNIGLRFGLALMMLMMAAAFFNDVTRLIG"
     gene            complement(14320..15555)
                     /gene="ispC"
                     /locus_tag="ACFB64_000874"
     CDS             complement(14320..15555)
                     /gene="ispC"
                     /locus_tag="ACFB64_000874"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC1209260.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAAGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAIFQVLPRDYTDRLNEHGIDSIILTASGGPFLTTDLSTFDSITPE
                     QAVKHPNWRMGRKISVDSATMANKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGKLDFGALSALTFQKPDFGRFPCLKF
                     AYETINAGGAAPCVLNAANETAVAAFLDGQIKFTDIAKTVAHCLAQDFSNGMGDIEGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(15560..16357)
                     /locus_tag="ACFB64_000875"
     CDS             complement(15560..16357)
                     /locus_tag="ACFB64_000875"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC1209261.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALTALWEYA
                     RMAGLCKTETNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLLMPFWFALVSLRPHPDDALPLLAVMGLVWVADVCAYFSGKALGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAIMSVLN"
     gene            complement(16360..17106)
                     /locus_tag="ACFB64_000876"
     CDS             complement(16360..17106)
                     /locus_tag="ACFB64_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC1209262.1"
                     /translation="MKSSTQTILEHTAIPRHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDET
                     ALDAAVASFQKRERRFGRTSEQLPIGQQRN"
     gene            complement(17162..17719)
                     /gene="frr"
                     /locus_tag="ACFB64_000877"
     CDS             complement(17162..17719)
                     /gene="frr"
                     /locus_tag="ACFB64_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC1209263.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYIAEADKVLAAKEEDLMAV"
     gene            complement(17861..18853)
                     /locus_tag="ACFB64_000878"
     CDS             complement(17861..18853)
                     /locus_tag="ACFB64_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1209264.1"
                     /translation="MKIISLFSGCGGLDLGFEKAGFEIPAANEYDKTIWATFKANHPK
                     THLIEGDIRKIKEEDFPEEIDGIIGGPPCQSWSEAGALRGIDDARGQLFFDYIRILKS
                     KQPKFFLAENVSGMLANRHNGAVQNLLKMFDGCGYDVTLTMANAKDYGVAQERKRVFY
                     IGFRKDLEIKFSFPKGSTVEDKDKITLKDVIWDLQDTAVPSAPQNKTNPDAVNNNEYF
                     TGSFSPIFMSRNRVKAWDEQGFTVQASGRQCQLHPQAPKMEKHGANDYRFAAGKETLY
                     RRMTVREVARIQGFPDNFKFIYQNVNDAYKMIGNAVPVNLAYEIAAAIKKTLER"
     gene            complement(18858..19373)
                     /locus_tag="ACFB64_000879"
     CDS             complement(18858..19373)
                     /locus_tag="ACFB64_000879"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HFC1209265.1"
                     /translation="MTACLQKPAKMRKNGKRKTLSIIVGVVDKKKNLKHLAMVYGIDY
                     CADAECYLKIKNQIKEGIGNIGGIQFAETKELGRVNRIDPLNITYLRVRGMWGIENPW
                     FVFNYIYQRNMEKSFNFMAIINEDKWNSFNNTDKLLAIQDSKLAISDIKIKNPNNPAR
                     LRNAKLITYHL"
     gene            complement(19291..19695)
                     /locus_tag="ACFB64_000880"
     CDS             complement(19291..19695)
                     /locus_tag="ACFB64_000880"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HFC1209266.1"
                     /translation="MNIIDAIINLANNPVVGVESHSQSNNRANQAGDALEEYVKDLFS
                     GSFNLNETQRIARHAKVFSYLGNNSNPPDAMLRNGDAIEVKKIESKDSALALNSSHPK
                     SKLSVDDSMLTKACKDAEKWEEKDIIYNCRRR"
     gene            complement(19804..20931)
                     /locus_tag="ACFB64_000881"
     CDS             complement(19804..20931)
                     /locus_tag="ACFB64_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC1209267.1"
                     /translation="MNSSQRKRLSGRWLNSYERYRHRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTIGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLADCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPSMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTAALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(21001..21076)
                     /locus_tag="ACFB64_000882"
     tRNA            complement(21001..21076)
                     /locus_tag="ACFB64_000882"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21041..21043),aa:Thr,seq:cgt)
     gene            21183..21662
                     /locus_tag="ACFB64_000883"
     CDS             21183..21662
                     /locus_tag="ACFB64_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209268.1"
                     /translation="MFVNEKYPYATLFAGLVFLTLPFALAVHDAFACAFGRAGLLVSV
                     SDGGFGRRGGWDGTVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAVAWIFKLLLGSAALGGLSGEAVSEYAFAVWLVSMLTLPKRLTRAPVQPVVFHRKK"
     gene            21757..22380
                     /gene="rsmG"
                     /locus_tag="ACFB64_000884"
     CDS             21757..22380
                     /gene="rsmG"
                     /locus_tag="ACFB64_000884"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC1209269.1"
                     /translation="MERKERLRAGIAAMGPDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPYIEGAQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLRQAAIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            22480..23253
                     /locus_tag="ACFB64_000885"
     CDS             22480..23253
                     /locus_tag="ACFB64_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC1209270.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELAARVSGK"
BASE COUNT         4720 a         5888 c         6858 g         5947 t
ORIGIN      
        1 ttcactccac tttcgtacat gcctcacggg aagttccgtc ttcggacact aatttgcctg
       61 atttatccag ttgatacaca tcatcttttc cgggtttgtc catctgcgcg accaatccgg
      121 tgtacatcat gctttccaag agtcgtgcat cctccgactt ttctaaccac gccagcgttt
      181 cggggctgtg ctgtcgtgaa acttcaccgt acttgatttg gctttgagtt aaggcattgt
      241 tcgcaaaacg ccatttacct tcaccgacaa cacggtatgc caagatattg ccgtcatcat
      301 cgattttgag tatagcatcg ccacggaagc tgccgtcctg aagatatttg acttttgtat
      361 cactgtgaat gttttcatca gtgccgatgc aatgccatgt accgacaatc ggagaagcca
      421 aggcggttgt tgcggataaa agcaccggca gaataaagcg tttcatctat ttcctttttt
      481 aaaggaggcc gtctgaaaac cccggtttca ggttttcaga cggccttgtc tattcaatca
      541 aatcagtctt tcaacttcgc caactgattt tgcacttttg ccattttgtc ttccaattcc
      601 gccaaatcgg ctttgtcttt ttccaccaga tgcgccgggg ctttttcggt gtagccgggt
      661 ttggagagtt tggcattgag tttgtccaag gctttttgca gcttctcggc ttctttgctc
      721 aaacgggcgg tttcggcggc tttgtcgatt tcgactttca gcatcaggcg cgcgccgttg
      781 cagacggcga cgggcgcgtc tccgctttcg ggcagggcgg cgacttgccg tgcttcggtc
      841 aggcgggtca tcatcggcag gtatttgagg tagtccgcca agtcgtccgc gctttcgaca
      901 aacagcgggg ctttcacgtt gggctggatg cccgtttcgc cgcgcaggtt gcggactgcg
      961 ccgatcaaat cctgcaacac ggtcatttgc ccgaatgccg tctgaacaat ctcgccgccg
     1021 tcggtttcgg ggaagcgggc gagcatgatg ctgtcggcgg ttttggcatc gcacatgggg
     1081 gcgacggttt gccacagttc ttcggtgatg aacgggataa tcgggtgcag caggcgcagg
     1141 gcggcttcga gtacgcgcaa taaggtatgg cgtgtggcgc gctggcggct ggcgcagccg
     1201 gtttggagct gcactttggc gagttccaga taccagtcgc aataatcgtt ccatacgaag
     1261 ctgtacaggg tttccgccgc caaatcaaag cggtaggttt cgtaggcttg cgtaacctgc
     1321 tcgatggtct gattcagacg acctatgatc cacatatcgg ggaaggaatg gccgcgcggt
     1381 tcggcagcgg ttgcgccgta gccgcagtct tggttttcgg tgttcatcaa gacgaagttg
     1441 gtggcgttcc agattttgtt gcagaagttg cggtagcctt cggcgcgttt gaagtcgaag
     1501 ttaaccgaac ggcccaagct ggcgtagctc gccatggtga agcgcaatgc gtccgcgccc
     1561 atgctgggga taccttcggg gaagagtttt ttcgtggctt cttccacttt cggcgcggtt
     1621 tcgggtttgc gcaggccggt cgtacgtttc atcagcagct tgtccaagcc gataccatcg
     1681 atcaaatcca cagggtcgat gacgttgcct tcggatttgg acattttttt gccttcgtgg
     1741 tcgcgcacga tgccgtggat gtacacggct ttaaacggta ctttgccggt gaagtgggtg
     1801 gtcatcataa tcatgcgcgc cacccagaag aagatgattt catatccggt aaccaagacg
     1861 ttggacggca ggaaggcttt gagctcgtcg gtttcagacg gccagccgag cgtggagaac
     1921 ggcacgagcg cggaggagaa ccatgtgtcc aatacgtctt cttcgcgggt caaacctgtt
     1981 ttgccggctt gtttttcggc ttcagcctga ttgcgggcga cgtaaacatt acctgcttcg
     2041 tcgtaccacg cggggatttg atgcccccac cacagttggc gcgaaataca ccagtcttgg
     2101 atgttgttca tccattggtt gtaggtattg acccagtttt cagggataaa gcgcactgcg
     2161 ccgctgtcga cggctttttt ggctttgtcg gcaaggctca agcctttgaa ttcgttgtca
     2221 ggctcgccgc cgttcggtgt ggcggacatg gcgacaaacc attggctggt cagcatcggc
     2281 tcaatcactg aacctgtgcg gtcgcctttc ggcgtcatca gcgtgtgcgg tttgatttcg
     2341 accaagaagc cttgctcctg caaatcggca accatttgtt tgcgcgcggc aaagcggtcc
     2401 aagcctgcgt atttttcagg cagggaaaag cctggttgcg cttcgccttt gaagttgaac
     2461 acttcggcgt ttgccagcac tttggcttct aagtcgaaca cgttaatcag gcgcgtgtcg
     2521 tggcgtttgc cgacttcgta atcgttgaaa tcgtgcgcag gcgtgatttt cacgcaaccg
     2581 gtaccgaagt ctttttcaac gtattcgtcg gcgataaccg gaatggtgcg accggtcagc
     2641 ggcaggatta attctttgcc gattaagtgg gtgtaacgtt catcttctgg attgacggca
     2701 acggccgcgt cgcccagcaa cgtttcagga cgggtggtcg ccacgataac ggcttcggtg
     2761 ggattgtcgg ccagcggata gcggatgtgc cacatagagc cttgttcttc catgctttcc
     2821 acttccaaat ccgataccgc cgtgccaagc acgggatccc agttcaccaa gcgtttgccg
     2881 cggtaaatca agccctgttc atacaggcgc acgaacactt cggtcacggt ttcggcgcgt
     2941 acgccgtcca tcgtgaaata ctcgcgcgtc cagtcggcgg agcagcccac gcggcgcatt
     3001 tgctgggtaa tcgtgccgcc ggaaacttct ttccattccc acactttttc caagaatttt
     3061 tcgcggccca agtcatgacg ggacacgttt tgcgcggcaa gctgacgctc gaccacgatt
     3121 tgcgtggcga tgcccgcgtg gtcggtaccg ggaatccagg cggtgttgca gcctttcatg
     3181 cggtagtagc gggtcaggcc gtccatgatg gtttggttga aggcatgacc catgtgcagc
     3241 gtgccggtta cgttgggcgg cggcagttgg atggaaaaag acggtttggt caaatccata
     3301 tcgggccgga aatagccttg gctttcccag ttttgatagt gtttggattc gatttcggcg
     3361 ggggagtatt tgtctaacat atgaagccct gagaaataag atgatttgat ggtttgaatt
     3421 atagcgcaaa tgccgtctga aacgctttcg gggtttcaga cggccttaaa tttaaatatt
     3481 gcctttacag tttttgatag cgcgacaggc tcaaatcgtc gctgcggatt tcggtgtctt
     3541 tgccgctcac gatatcggcg gtcaattttg ccgaacccgg cgacatggtc cagcccaaag
     3601 taccgtgccc ggtattcaga aacaggtttt caaagcgggt gcgcccgatt aacggcgtac
     3661 tgtcgggcgt catcggcctg aggccgctcc aggacaatgc ttggctcaaa tcgccgcctt
     3721 ccgggaacaa gtcgttgacg accaaagcca aggtttcgcg gcgtttttcg ggcagtttgg
     3781 tttcgtagcc ggacaattcc gccattccgc cgacgcggat tctgttgtca aaacgcgtga
     3841 tggcgacttt gtagctttca tctaaaacag tggacaccgg tgcgccgtcg gaatttgtga
     3901 ccggcagggt caaggaatag cctttgacgg gataaatggg cagattgaga tccaactgcg
     3961 ccaacacagt cctgctgaag cagccgagcg cgcagacaac ggcatctgtt tcaaaccgcc
     4021 ctgtttcggt ttcaacggct ttgatgcgca gcccgttgtg gtcgatgcgg ctgatggttt
     4081 ggttgaagta gaaccgtacg cccttctctt gacacaattt gtacaggttt tcggtaaaga
     4141 ggcggcagtc gccggtcgca tccgcaggca ggtgcagacc gccgacaatt ttggcggtaa
     4201 cgcgtgccag cgcaggctcg aattctgcgc attcttcggg cttcagacgg cggtacggca
     4261 cgccgtagcg ttccaaaacg gcaatgtctt gttttgccgc ttcgacttct tcggtttggc
     4321 ggaaaatctg caacgtccct tttttgcgtc cctcaaaatt catgtcggtt tgcgcttcaa
     4381 aacggcggaa catttcacgg ctgtattcgg aaatcctgac catgcgctct ttattgattt
     4441 gatagcgcgt tgccgtgcag ttttgcagca tccgccacag ccattcgatt tgatacaggc
     4501 cgccgtcagg gcggaacagt aaaggcggat ggcttttaaa cagccgtttc agtgctttgg
     4561 tcggaatacc gggtgcagcc caaggcgtgg tatagccgta agaaagctgg cctgcattgg
     4621 caaaactggt ttccatcgcc acaccctcgg tgcggtcgat gaccgttact tcatgtccgg
     4681 cctctgccag ataccacacg gaggatacgc cggcaatacc cgcacctaaa acaagcactt
     4741 tcatgtttcc ccctccggct ttttcaaaac aggcttaata tgccgtgccg tctgaatatt
     4801 cggattcaga cggtctcgga tattaatgcg gcaattcgcc gtttgtgatt ttttgtttga
     4861 agtcgcgtgt ttcattgacg atgactttcg ccatcaacaa aagcgcagtc aggttgggca
     4921 atgccatcaa gccgttgaat gtatccgaag ccagccatac caaatcaagg ctcaacacgg
     4981 tacccggcat gacggaagaa acataaccga cgcggtacaa agcggcaaac ttctcgccga
     5041 agacgtaaac cgcgcatttc tcgccgtaat aacaccagcc gagaatggtt gagtaggcaa
     5101 agaaaatcag gccgatggtc acaatccagc cgccgatgcc gggcagcatt ttttggaaag
     5161 tgacggttgt cagcgccgcg ccgctcaatt caggtttcac aaactcgccg cctgcgccga
     5221 gcagacccat gaccaacacg ataccggtaa tcgagcagac aacaatggtg tccaaaaaag
     5281 taccggtcat ggaaaccaaa gcctgacgaa cggggtggtc ggttttcgcg gctgcggcgg
     5341 caataggcgc agaacccata cccgcctcat tggagaacac gccgcgcgcc acgccgtagc
     5401 ggatgaccgt accgatagcg ccgcctgcta cggcctgcgc gctgaacgca tcggagaaaa
     5461 tcagcttgac ggcaggcatc agtgcatcgg aattaatgac gataatggaa agaccgccca
     5521 acacataaaa caccgccata gcaggcacga tgaaagaagc ggctttggcg atgcctttaa
     5581 taccacctaa aacgacaacg gcagtcagaa cggtcaacgt aatgccggta taggcaggtt
     5641 cgataccgaa gctggtttgc acagcctgtg caaccgagtt ggactgcacc gagctgccga
     5701 taccgaagga agcgaatgtg ccgaacagcg caaatgcaac ggccatccat ttccagtttt
     5761 tgcccaagcc tttttcgatg taatacatcg ggccgccgga catttcgcct ttggaattgt
     5821 tgacgcggta tttcaccgcc aacacgcctt cgccgtattt ggtggccatg ccgaaaatag
     5881 cggtcatcca catccagaat accgcgcccg ggccgccggt taccactgca gtcgccacgc
     5941 cggcgatgtt acccgtaccg atggtggcgg acagcgcggt catcaacgcc gcaaaatggg
     6001 aaatatcgcc ttcgtggcct gtgccgcctt catacttctt tgacggcaca aacgcctgtt
     6061 tcagcgcata gcccaacata gtgaactgca agcctttcag cataacggtc agcacaatgc
     6121 cggtgccgac cagcagcatc aacataacag atccccaaac ccagctgctg acggtttcga
     6181 aaaaggcttt gagattgtct aaaaatactt gcatggcttt ctcctttgtc tgttttattt
     6241 ttaaaacacc acttttgtag tgtccggtaa tttcagcaca gaatattcaa taagacaata
     6301 tgttcttttg aaaaatactt ttggtttttt caccgaagac aggccggttc aagctgcgga
     6361 aattgtttgc aattatttaa aagcagaggc ggaggtcaca atgaaatgtc cgaatgggga
     6421 tgtggcgggc ggcagaaatc atcaatgctg ccgactgcca tacttttgaa atctatagtg
     6481 gtttaaattt aaaccggtac ggcgttgcct cgccttgccg tacttcttgt actgtctgcg
     6541 gcttcgtcgc cttgtcctga tttaaattta aaccactata caaaatgatg catcgatcaa
     6601 acaacatacc gctttaaaaa aaaccgatgc cgtctgaaac gctttcgggg tttcagacgg
     6661 cattgaaagg gtgcggtcag cggatgatgc cgcgcgtcga ttgtgcgaag aagtcttgaa
     6721 atacggcaag ctcggcttgg gtttcggcgc ggcggagaat gtccgccttg gcttcttcaa
     6781 acggaatgcc gcgatggtag agggttttgt atacgtcttt gacggcggaa atctgttctg
     6841 cggtaaaacc gttgcggcgc ataccttcgc tgtttaggcc ggccggttcg gcgcggtagc
     6901 ccgatgccat aaagtagggc ggcacgtctt tgtgtacgcc tgcggcgaag gcggtcatgg
     6961 cgtagtcgcc gatgcggcag aattggaaga ccagcgtgta gccgcccaaa acgacgtagt
     7021 cgccgacggt aacgtgtccg gcaagcgagg cgttgttggc gaaaatggtg tggttgccga
     7081 ccacgcagtc gtgcgcgagg tggcagtacg ccataatcca gttgtcgtcg ccgatacggg
     7141 tttcgccgat gccggttacc gtgcctaaat taaaggtggt gaattcgcgg atggtgttgc
     7201 cgttgccgat aatcagcttg gtcggctcgt cgcggtattt tttgtcctgc ggaatttcgc
     7261 cgaggctggc aaattgaaaa atgcggttgt tttcgccgat ggtggtgtgg ccgttgatga
     7321 cggcgtgcgg accgatttct gtattcgcac cgattcggac gttggggccg atgacggtgt
     7381 acgcgccgac tttgacgccg gagtcgagtt cggctttggg gtcgatgacg gcggtcgggt
     7441 ggatgagggt catgttttga atactccgct gcttgttaag acaaataata gaaaatcaca
     7501 gtttgtcgat ttcatcgcag accgcaatca cttgatcgct aaaatggcta aatactgaag
     7561 cagtatctac atccatgtaa ttaagcagtt caaaaataga ttggtaaata tttctagcgt
     7621 agccggtatc ttttattgca cctttaaaac aaatcggttc gtgatgtgcc aaacgattac
     7681 ggaaattgtt aatattggaa agttctcgaa aaatccattt ttggttatat tgcacacttg
     7741 gtgtagattt gggctttttt ggaaaaactt tcatcaatac ttttcctgca gcatcaaatt
     7801 gagcatcttt ccctcccgca aacagatacc gccaaaaacc gaatcctagt cctgcaacca
     7861 attggttgtg gtcaaacttt cctccgctat tattttgtat tttagataat gcaaccttga
     7921 ttaattcagc agattctttc gtaccatttc ttaaaaagca accttgatat ttcaatgcag
     7981 gattcgtttg tggttgtatg ctatcatata accggtttct gtctttaaac tcctgccgaa
     8041 agcaaatatc aattttgttt cttaaaacga tctcaaatat agaaacaaca tgaaaaagtt
     8101 ctcttgataa agataaattc aataaataca attcagctgc tttgtctttc ctaacggcat
     8161 ttttataccg ttccattctg aaatcagaaa aaatactaca gcattcctta taattcacgc
     8221 taacccttga ttctttattt ttaaattgac gtttcaaact atatttgata tagttagata
     8281 aaactaatcc ccaccgtgtc cctgggtttg aataaaccta aaccggtggg ttttcttttt
     8341 attaggtcgt ctgaaacttt cagacgacct ttttccgaac gctcaaacca cgcgtttggc
     8401 gcacataatc acggcttcga cggcgacttg tccgtccact ttggcaacgg cgttgaattt
     8461 gccgatgccg cgccggctgg tcaggagttc gacttcaaag acgagttggt cgccggggat
     8521 gacttggcgt ttgaaacggg cttcgtctat gccggcgaag aagaaaaatt cgttttcctt
     8581 gcgcccgcct tcgctcaaaa tcgccaacgt gccgcacgcc tgcgccatcg cttcgatgat
     8641 gagtacgccg ggcataacgg gcaggtcggg gaaatggcct tggaattggg gttcgtttat
     8701 ggttacgttt ttgatggcgg tcagggtttt catcggctcg aaggcggtga tgcggtcgag
     8761 ctggagaaac gggtagcggt gggggatgag tttttggatg tctttggctt cgatggggag
     8821 ttgtacgtcc atgtctgtcg tattccttga ataaagtcgg tttggattta ttgcgtgtct
     8881 ttgctgtctg aaagctgctg ttccagcgtt ttgaggcgtt tgttcatttc gcttaagcgg
     8941 tggatgtaaa cggcgttgcg tgcccattct ttatgctcgg acatcgggaa gatgccggcg
     9001 atgtgtttgc cgctttcggt aatgctgtgg gtgacggacg tgccgccgcc gatggtggtt
     9061 ttgtcggcga tttcgatgtg tccgaccgta ccgacgccgc cgccgatgat gcagtagctg
     9121 cctatggtta cgctgcctga gatgccggtt ttggcggcga tgacggtgtg cgaaccgatt
     9181 ttgcagttgt gtccgatttg gacttggttg tcgattttgg tgccgttgcc gacggtggtg
     9241 tcgctcatcg cgccgcggtc gatgttggtg ttcgagccga tttctacgtc gtcgcccagc
     9301 gttaccgcgc cggtttgcgg gattttgaac cacgaatcgc cggcgaaggc gagtccgaaa
     9361 ccgtccgcgc cgatgaccgc gccgctgtgg atttcgacgt gtctgccgag tgtgcagccg
     9421 taataaacga cggcgttggg atgcaggacg acttcgtcgc ccagtttgca atcgtgttgg
     9481 acgacggcgt ttgccaagat gcggcagcct tcgccgagca cggtgttcgc gccgatgtag
     9541 gcgttcgcgc cgatttcaca gcttgcggga acggtcgcgc ccggttcgac gacggcggtc
     9601 ggatggatgc cgccgcgcgc tttgacgacg ggtgaaaaca ggcgcgcgac tttggcgaaa
     9661 tagagatagg gatcgtcggc gacaatcagg ttgcgccctt caaatccgtc tgctgctttg
     9721 gcggaaacga tgatcgcgcc cgcgctgctg tcgtggactt cggctttgta tttcggattg
     9781 gcgaggaagc tgatgtgttc cgcctgcgcg tctgcgagcg ggcgcacggc ggtaacggaa
     9841 atgtcctcgc cgcgccattc gccgccgagc cgcgcggtga tttgggacag ggtgcaggtg
     9901 gccggaatca tggttttcct gtttggtatg ccgtctgaaa gggttagcgg gcgttcattt
     9961 ctttaatgac gctgtcggta acgtcgtatt gggtgttgac gtaaatcacg tcctgcaaaa
    10021 tgacatcgta accttcctgt ttggcgattt tgacgatgac gcggttggcg ttttgctgga
    10081 gggaggcaaa ctcttcgttg cggcggaggt tgtagtcttc ttcaaactgc gcctgttttt
    10141 tgcggaacgc ttcgaccagc ccgcgccatt tttcttcggc ttgcgccttt tttgcgtcct
    10201 taagtttgcc gccggcgagc tgcctttcca aatccaagcc ttcgcgttgc agtttttgca
    10261 attcgtcctg acgggcggaa aattcgccgt ccagcgtttt ttggatgttg cgcgcctgct
    10321 tggattcgag gtagatgcgc tcggtgttga taaagccgat tttttggaag gtgtcggcgt
    10381 gcgtgcctgt ggtgcagcat aaaccgatca gggtggcggc aaacgcgcgg gtcaaacggg
    10441 tcatggtaaa actccttcgg atgttgccgc gaaatgccgt ctgaagggct tcagacggca
    10501 tttgcgggat tagaacgtcg tgccgagctg gaattggaag cgttggattt cgtcttccgg
    10561 ttttttcttc agcgggtagg cgtagctgaa tttcatcggg cccaaaggcg agagccaggt
    10621 aaccgcgccg ccggcggaat agcgcaattc gttggtaaag gtggatttat gcgcgttttc
    10681 cgagtaaacc gatttgttgt taccgttttc ggcggcggta taggttctgc cgtcccacac
    10741 gctgcctgcg tcggcaaaca ggctcaggcg gacggtgcgt gcgtctttcg caccgggcat
    10801 cgggaagagc agctcggcgg agacgttggc ttttttgttg ccgccgtagc tgattttttc
    10861 gccgtattcg tcatacactt tcgggccgag cgtgccgctt tcgtagccgc gcaccgaacc
    10921 caggccgccg ccgtagaagt tttcaaagaa ggggatttct ttggttctgc cgtagccgcc
    10981 cgcaatgccg acttcgccgc cgagcatcag cgtgaaggtt ttgcttaagg ggaagaacca
    11041 ggtttggttg tgggtggcgg agtagtattg cagtttgctg ccgggcaggg cgatttcggc
    11101 atttacgccg gtcaggtagc cgcgcgtcgg ccataaggcg ctgtcggtct tgttgcgccc
    11161 ccagccgaca gtgcctttgt acagcaggcc tttgaagctg ccgtctgcgc cgtcggtttt
    11221 gccgtattgt ttgataaagt cggcatagcg tttgggtgct ttgttgtagg tgttgacggt
    11281 caggtgttcc gccgccagcc cgaaattgac gcggtcgtat tcggtaacgg ggatacccat
    11341 ccttacgccg ccgccggcgg tggtggtttt atattgtttg acgctggtcg atgctttgcg
    11401 cgggtcgaag gcttttccgt aaatatcgta gcccaggctg accccgtctg ccgtgaagta
    11461 cgggtcggta aacgacagcg agccgttgag cgtggttttg cttcgcgagg cgcgcagggc
    11521 ggccgacttg cccgtaccga acaggttgtc ctgcgatacg ccggcggaca tgaccaagcc
    11581 ggtatcctga acccagcccg cgctcaagtc gagcgagccg gtggagcgtt cggtcaggct
    11641 catgttcaaa tcgactttgt cgggcgtgcc ggcaagcggg acggcatcaa actgtacgtt
    11701 gtcgaagtag cccaaaagct cgacgcgctc tttggaacgt tgcagcttgg aggtgtcgta
    11761 aggcgcggat tccatttggc gcaattcgcg gcggacgact tcgtcgcggg ttttgttgtt
    11821 gccggtgatg tggatttcgt tgacgtagat ttttctgccc ggttcgatgt gcaggacgaa
    11881 atcgacggtt ttggttccgg cgttcggcag cggctgtacg ctgatttcgc tgtatgcgta
    11941 gcctgccgag cccatgcggt tctgaatctc acccaaaacg gcggtcatct gctggcgttc
    12001 gtaccatttg ccgggcttca tggtcagcag tttttccagt tcggccttgg ggacttcgtt
    12061 ggtgtcgcct tcaatcgaca ctttgcccca gcggaaacgt ccgccttcgt ggacggtgat
    12121 tttgatggtc tgcctggttt tgtcttcgtt ggtttggatg tcggtatcga ggatacggaa
    12181 atcgaagtag ccgttgttct ggtagaagtc ggttactttt tccatgtctt gggcgaattt
    12241 ctggcggtcg aaccggtcgc ttcgtgtcag ccatgtccaa atgccgcctt cggtcagcga
    12301 catctgccgc atcagtttgc ggtcggaata gacttggttg ccttcaaatt cgatgtcggt
    12361 gattttggcg gatttgccct cgtcaatcgt gatgtcgatg tcgacgcggt tgcgggcgag
    12421 tttggttact ttgggcgtga tttggatatt gagtttgccg cgcccgaggt attcttcttt
    12481 caggccggcg actgcctggt tgagtgtcgc ctgattaaag tattgcgact gcgccagccc
    12541 gaacgattcg aggtttttct tgatggcgtc gttttgcagc attttggcgc cggtgatgtt
    12601 gagcgagccg atggtggggc gttcgataac ggtcagcaga agctgcccgt ccgcagtttc
    12661 gactcgtacg tcgtcaaaga aaccggtggc gtacaggctt ttgatgatgg cactgccgtg
    12721 tgtgtcgttg taggtgtcgc cgactttgac gggcaggtag ttgaatacgg tgctcggctc
    12781 ggtacgctgc aagccttcga cacggatgtc ttggatggtg aagtcggcaa atgccaaagg
    12841 cgatatgccc aacatcatca gtgcggaggc aatctgtttc agtttcatcg taagttcctt
    12901 gtggtgcgga atgcggtttc agactgcatt ccgaaacgta aaatctaacc gatcagccgg
    12961 gtaacgtcgt tgaagaaggc ggccgccatc atcagcatca tcagggcgag cccgaagcgc
    13021 aaaccgatgt tttggacacg ttcgcccaaa ggtttgccgc gtatccattc gacagtataa
    13081 aacacgaggt gcccgccgtc caaaacgggg acgggcagca ggttcagcac gccgaggctg
    13141 atgctgacca acgctaaaaa ttccaaataa ctttgcaagc cgagttcggc ggactgtccg
    13201 gcaatgtcgg caatggtcag cggcccggaa atatggctga cagaggcgtt gccgctgatt
    13261 agtttgccga aaaatttgag ggttgtccac gagtgggaaa cggttttttc ccagcccatg
    13321 ccgaatgcgc ggacaacaga cggacggtag ctgcggcgga tttgcgcgtc ccacgccctg
    13381 tccggctgcg gacggaggcc gacgcgcccg atcagggtgt ggtcgggctg ttcgacagta
    13441 tcggggcgga tgtcggcggt atgggtttgt ccggcgcgtt cgtaggtcag ggtgattttt
    13501 ttgcccgggc tttggcgggt caggtttgcc cattcctgcc atgaggcgat gggtttgccg
    13561 tcggcggcag tcagcctgtc gcccggtttc aggcctgctt tttcggcggg gctgcctttt
    13621 tccacgccgc cggcaacggt tgtgatttta aagggcatca gtccgatgta gccttggttt
    13681 tttgcgattt taccggcttc cggcgtgcct gcggcatcga tggtgcggac ggtttgcgcg
    13741 cccgatgccg tctgaacgcc gacggcgact ttgccggctt cgaggttgag gacgatttcg
    13801 gtttgcgcgc tgctccagtc ttggacggaa acgccgttga cggattgtat tttgtcgccg
    13861 ctttggaagc cggtgcgggc ggcaatggtg tcgggttcga ctgtgccgac atagggccgc
    13921 agttcggtta cgccgaagga aaagctcagt ccgtacagca aaaccgccag tgcgaggttg
    13981 gtcagcggac cggcggcgac gatggcgatg cgcttggcgg ggtgttgttt gtcaaaagcg
    14041 tagggtaaat cggcttctga tacttcgcct tcgcgcgtat cgaccatttt gacgtagccg
    14101 cccaacggaa tcggggcgag gcaccattcg gtgtcgccgc gctttcgggt gaaaaacggt
    14161 ttgccgaagc cgacggaaaa acgcacaacc ttgacgccgc acaacctggc gacgatgtag
    14221 tgtccgaatt cgtgcaggct gaccaaaatc aggatggcga agataaaagc tagaagggtt
    14281 tgcaaatggt tttcctttga taacggtgtt cggacggtat cagcgcagtg tgccgataaa
    14341 tgcccgcgct tgtgcgcgtg tccgggcatc ttgcgccaac agtccttcta tatcgcccat
    14401 gccgtttgaa aagtcttgtg caagacagtg ggcgacggtt ttggcaatgt cggtaaactt
    14461 aatctgtccg tccaaaaagg cggcgacggc ggtttcgttg gcggcgttca atacgcaggg
    14521 cgcggctccg cctgcgttta tggtttcata ggcgaacttc aggcagggga agcggccgaa
    14581 gtcgggcttt tggaaggtca gcgcggacaa tgcgccgaaa tcgagtttgc cgacacccga
    14641 atcgatgcgc tcgggcaagc ccaaacaata ggcgatgggc gttcgcatat cgggattgcc
    14701 cagttgcgcc agcacggagc cgtcgcggta gcgcaccata ctgtgtatca cggattgggg
    14761 atggatgacg acttcgagtt tgtcgggcgg acagttgaac agccaatgcg cttcaatcag
    14821 ttccaagccc ttgtttgcca tagtggctga atcgacggag attttgcgcc ccatacgcca
    14881 attggggtgt ttgaccgcct gctcgggcgt aatgctgtcg aacgtgctta aatcggttgt
    14941 taaaaacggg ccgccggaag cggtcaggat aatcgaatcg atgccgtgtt cgttcagacg
    15001 gtctgtgtaa tcgcgcggca aaacttggaa aatggcgttg tgttcgctgt cgacgggcaa
    15061 cactgccgcg ccgtttgcgc gggcggtttc cataaacaac gcgccggaaa ccaccagcgt
    15121 ttctttgttt gccagataaa tggttttgcc tttttgcgcc gctgcgagcg cggaaggcag
    15181 ccccgccgcc ccgacgatgg cgcacatgac gccgctgact tcgtcggcag acgcaacgtc
    15241 aaccaatgcc tgcgcgccgt gtaaaacctg agtcgccgtg ccgtcgcgtt tcaacagggc
    15301 ttcaagccgg gcggcgtgtt cggcatcggc aacgacggca tattcggggc ggaacgtttg
    15361 acattgagcc gccagtttct cgacctgctt atgccctgcc agcgcgaata cgcggaattt
    15421 ttcgggatgg cgggagacaa cgtccagcgt gctttcgcct atgctgccgg tactgcctaa
    15481 tatggtcagg acttgtggtg tcataatgag gataacttta taccggatgc cgtctgaagc
    15541 gttttcagac ggcatagaat caatttaaaa ccgacattat cgccgcatag acgctgataa
    15601 cggcaatcag gctgtcggta cggtcgaaca cgccgccgtg tccgggcagc aggttgctgc
    15661 tgtctttgat gcctgccgcg cgcttgagcc agctttccaa caggtcgccg catacgctga
    15721 caacggtcaa caccaaaccg attaacacgg tatcgaacca gcctgtatcg aatgccagcc
    15781 agccggcact tcgtacggca gtcatgtaca ctgccacgca aaccgcaccg ccgattgcgc
    15841 cttcccagct cttgccgggg ctgattgccg gtgcgatttt gtgtttgccg agcgccttgc
    15901 cgctgaaata cgcgcaaacg tcggcaaccc agaccaaacc catcacggcg agcagcggca
    15961 gggcatcatc gggatgcggg cgcagggata cgagcgcgaa ccaaaacggc atgagcaaaa
    16021 gccagccgac ggcataaacc tgccaaccgc cgttgagcct ccatttgaat ctcaaccaca
    16081 aaggcataac ggcgagccaa aatgccaaaa caacatacca aaccaaatta ggcagcatcc
    16141 agccgcccgc ataggcaact acgccgaaaa ccaaggttgc ggcgaggtaa tggttggttt
    16201 cggttttgca caaaccggcc atacgggcat actcccacaa ggcggtcagg gcaatcagcc
    16261 cgcaaaatgc agcccacagc cattgcggcg cgtaaaacag catgcccagc atcagcggca
    16321 gcagccacat agcggttatt acccgttgtt tcagcatatt cagttccttt gctgtccgat
    16381 aggcagttgc tcggaagtgc gtccgaaccg ccgttcgcgt ttttggaacg aagcgacggc
    16441 ggcatctaag gcggtttcgt cgaaatcggg ccacaggatg tcggtgaaat acagttctgc
    16501 atacgccatc tgccagagca ggaaattgct gatgcgcgtt tctccgcccg tgcggatgaa
    16561 caaatccggt tccggcgcgt cgcccagcat caagtgtttg gccagcgtgt cttccgtaat
    16621 ctcggatacg ccttcggcaa tcagtttgtt tgccgcctgc aaaatatccc agcggccgcc
    16681 gtaatccgca gcaatgctca gggtcagacc ggtattattt gccgtcaagg cttccgcctc
    16741 ttcgatacct tgcagaatct gccgattaaa gcgttcgcgg ctgcccaata ttttcaggcg
    16801 catattgttt tcgtgcaggc ggcgtacctg tttttgcaaa gcctgtaaaa acagccccat
    16861 caggaacgaa acttcgtctt cggggcgacg ccagttttcg gttgaaaagg caaacacggt
    16921 cagatattgc acgcccagtt tggcgcaatg cttcaccata ttttccaacg cgtccaagcc
    16981 gcgtttgtgt cccattatac gcgggagaaa acgttttttc gcccaacggc cgttgccgtc
    17041 cataattacg gcgatgtgcc tcgggatggc ggtgtgttcc aaaatggtct gcgtgctgct
    17101 cttcatatct gcctttcgcg gttcggcgtt caaatgccgt ctgaacgccg cgccgtgaga
    17161 tttaaaccgc catcaggtct tcttctttgg cagccaaaac cttatcggct tcggcaatgt
    17221 acttgtcggt cagtttttga accgcctctt cgccgcgacg tgcctcgtct tcggaaattt
    17281 ctttgtcttt gaggagtttt ttgatgtggt cgttggcatc gcggcgcacg ttgcggatag
    17341 agacgcgtcc ttcttccgcc tcgccgcgca cgactttaat caggtctttg cggcgttcct
    17401 cggtcagcat gggcatcggc acgcggatca aatcgccgac agctgccggg ttcagtccca
    17461 agtttgaatc gcggatggct ttctcgactt tggccgccat attgccctca aacggtttca
    17521 cgccgatggt gcgcgcgtcc aaaagggtta cgttggcaac ctggctgacg gggaccatgc
    17581 tgccccagta ttcgacttcc acttggtcga gcaggccggt atgcgcgcgg ccggtacgca
    17641 ctttcgccag attttctttc agtacttcga ccgaacgctg catcttgcct tcggctgttt
    17701 tttgaatatc gttgatcata ttgttctttc ggtaagataa ggtaggcggg cagccgttcg
    17761 aacgcgctcc aagccgttca gacggcataa agaccgttaa ctgcgaatag taccgttatt
    17821 cgggcatgac gacaaggcag atgcctgtag gaagggtgtt tcacctttct agggtttttt
    17881 taattgccgc tgcaatttcg taggcaaggt tgacggggac ggcgttgcca atcattttgt
    17941 atgcgtcgtt gacattttga tagatgaatt tgaagttgtc gggaaagcct tggattcttg
    18001 caacttcgcg taccgtcatc ctccgatata gtgtttcttt gccggcagca aaacggtagt
    18061 cgtttgcccc gtgcttttcc atctttgggg cttgcgggtg cagttggcac tgcctgcctg
    18121 aagcttggac ggtaaaaccc tgttcatccc acgctttaac tcggttccgg ctcataaaaa
    18181 taggggaaaa actgccggta aaatattcgt tgttgttgac tgcgtcgggg ttggtcttgt
    18241 tttgcggggc ggaaggtacg gctgtgtcct gcaaatccca aataacgtct ttcaatgtaa
    18301 tcttgtcttt gtcttcgacc gtcgaacctt ttggaaaaga aaattttatt tccaagtctt
    18361 tacggaaacc gatgtagaag acccttttgc gttcctgtgc tacaccgtag tctttggcgt
    18421 tggccatagt caaggttacg tcgtatccgc atccgtcaaa cattttcagc aggttttgta
    18481 cggctccgtt gtggcggttt gccagcattc cgctgacgtt ttccgctaaa aagaattttg
    18541 gctgtttgct tttcaaaata cggatgtagt cgaaaaacaa ctgtccgcgc gcatcgtcga
    18601 tgccgcgcaa agctcccgct tcagaccaag actggcaggg tgggccgccg ataatcccgt
    18661 cgatttcttc agggaaatct tcttctttaa tcttgcgtat atcgccttct atcaaatggg
    18721 tctttggatg gtttgccttg aaggttgccc aaatggtttt atcgtattcg ttggcggcgg
    18781 ggatttcaaa tcccgctttt tcgaaaccca aatccaaacc gccgcaaccg ctaaacaaac
    18841 taatgatttt cataggatta taagtggtag gtgatgagtt ttgcatttct taatcgcgcc
    18901 gggttgttgg gatttttaat tttaatgtcg gaaatggcca gtttggaatc ctgtatggca
    18961 agcagcttgt ctgtattgtt aaaactgttc catttgtctt cgttgataat ggccatgaaa
    19021 ttgaaacttt tttccatatt ccgttggtaa atatagttga agacaaacca tggattttcg
    19081 attccccaca taccccgtac tctcagataa gttatgttga gcggatcaat gcggttgacc
    19141 ctgcccagct ctttggtttc tgcaaactgt atgccgccga tgttgccgat tccttcctta
    19201 atttgatttt ttatcttcaa atagcattcg gcatcggcgc agtagtcgat tccgtaaacc
    19261 attgccaagt gtttgaggtt tttctttttg tcaacgacgc cgacaattat agataatgtc
    19321 tttctcttcc catttttccg catctttgca ggcttttgta agcatgctgt catcaacaga
    19381 gagctttgat tttgggtggc tgctattcag cgccagtgcg gaatctttac tttcaatttt
    19441 cttaacttca atggcatccc cgtttcgcaa catggcatca ggagggttgg agttgttgcc
    19501 caaatatgag aatacttttg cgtgtcgggc tatgcgttgg gtttcattta ggttgaaact
    19561 gccggaaaac aagtctttta catattcttc caaagcatca cctgcctgat tggcacgatt
    19621 gttgctttga gaatgggatt cgaccccgac aacagggttg ttagccaaat tgatgatggc
    19681 atcgataatg ttcatggttt ttccttgggg ttttgcggca gattatagca aaaccgtcag
    19741 ggtgtttatc ggaccgatgc cgtctgaagc ggcaggcgtt tcagacggca tcgtgtccgg
    19801 ccgtcagccg tgttcccgtg tttcaagcag gctttggcgc aggtgttggc gttcgtgggc
    19861 atccagccat ttgcggcggg tgcgttgcag caggatgacg agggcggaaa tttcctgacg
    19921 catattggtg ctgagccaga ggaagccctg ccattggtag tggaggtgtt cggcgagggc
    19981 ttccagttcg gggttgatgg cggtgtcgat gcggatgcgg cgggcgtgtc tgccgttgat
    20041 gagggcggcg gtttgttgca ggtcggtttg gagcagtgtg aagtggcggt cgagcagccg
    20101 gatttcgctg ccgttgagtt tgggagattg cagcttggcg gcggtggtca ggagcagctc
    20161 ggtggtgttg acgattttgc ggtgggcgtg ctgcatggct tccatcatgg aggggctgat
    20221 gcggctttcg cccgatgtgg cggcgaggtg gctgcggctt ttgaccatgc gtgcgttgat
    20281 ttggcgcatt ttgaccatat tctgctccaa acgttcgcgc gtcatacgcc tgccgttgct
    20341 gatttcggca atcattttgc tgcagtcggc caggttgtcg gcaagcatga aacgccacat
    20401 cagtgtggat ttcagcggca gcagtttggc ggcggcaatg gcgatggcgg cgccgatgag
    20461 gacgttcatc gcgcgcatca ggccgctgtc gagccattcg ctgccgttgt cgccgatgag
    20521 catgcacatc gtcagccccg ccagcatagg gacgtagccg tttttgccga ccgccgccca
    20581 gccggccagt gcgcttgccg tgccgatggt caggtagaag aggaggttgc cgtggaaata
    20641 atgctggttc agccataaaa cgcccaaacc cgcgcccagc ccgatgaccg taccgagcat
    20701 acgttccacc gccttggagt aaatcgcgcc ttggaactgg agcatgccga ggacgacgaa
    20761 gacggtcatc cctatccatt cgccgtgttg gaggtggagt agccgggcga gtgcggtggc
    20821 gaacaggacg gctccgccga gccgcacggc atgtatgagg cggcggtggc ggtagcgttc
    20881 gtaggagttg agccagcggc cggaaaggcg tttgcgttgc gaggagttca tatcggatgc
    20941 cgtctgaagc ggaaatgtga aaaagcacag gcttcccgag ggagggtctg tgcttggtat
    21001 tggtgccgga gaagggaatc gaacccccga ccttcgcgtt acgaatgcgc tgctctaccg
    21061 actgagctac accggcgttt ttcgttatga tatatataaa cggttgtttg tgcaactttt
    21121 cgggcgggcg gcaaggcggt gcgcggtata atgcggcctg cttcggaaga ggggggacgg
    21181 cgatgtttgt gaacgagaaa tatccttatg cgaccctgtt tgcggggctg gtgtttttga
    21241 cgctgccgtt tgcgttggcg gtgcatgatg cctttgcatg tgcgttcgga cgggcggggc
    21301 tgctggtgtc ggtgtcggac ggcggattcg gccggcgtgg cggttgggac ggcactgttt
    21361 ggtttgtgtt cggtgtgttt gcgtttttga atgtggttgt gtcggcgggc ctgacgaaac
    21421 tggcgtacaa aaagatgatg cggcggcatt cgcgttacgc gctgtttctg tcgggcgtgg
    21481 cggcttgcgc ggcggtggct tggattttca agctgttgtt gggcagtgcc gccttgggcg
    21541 ggctgagcgg ggaggcggtg tcggaatatg cgtttgccgt gtggctggtg tctatgctga
    21601 cgctgccgaa acgcctgacg cgcgcgccgg tgcagcctgt ggtgttccac aggaaaaaat
    21661 aggacggaac ggaaatgccg tctgaaaccc gatacgcggt ttcagacggc atgtttttcc
    21721 gctaacatta cgcctgaata tggacaggaa gcagatatgg aacgtaaaga acgcctgcgt
    21781 gcaggcattg ccgcgatggg gccggatatt tcggaaacgg cgcaggacag gcttttggcc
    21841 tatgtggatt tgttgaaaaa gtggaacaaa acctacaatc tgaccgccct gcgcgacgag
    21901 gaaaaaatga ttgtccatca tcttttggac agcctgacgc tgctgcccta tatcgaaggc
    21961 gcacagacga tgctggatgt cggttcgggc ggcggccagc ccggcattcc ggcggcggtg
    22021 tgccgtccgg atgtgcagat aaccttgttg gatgcgaata cgaagaaaac ggcttttttg
    22081 cggcaggcgg ctatcgagtt ggggctggac aatgtgcgcg tggtgtcggg gcgcgtggag
    22141 gcggtttcgg acgtgcgtgc cgacgtggtt accagccgtg cgtttgcgga actggcggat
    22201 tttgtgtcgt ggacggcgca cctgttgaaa gacggcggct actgggcggc gatgaagggc
    22261 gtatatccgc agggggaaat cggccgcctg ccgcaggatg tgtgcgtcga aaaagtccaa
    22321 aggctcgacg tgccgggctt ggatgcggaa cgccatatcg tcatcctgag caagcgttga
    22381 gcgcgcttca gacggcatga ataccttttt tgtgcggata aaggtaaaat tccgcactgt
    22441 ttttcttttt ttcaacatca gacgggacac gggcgggaca tgagtgcgaa catccttgcc
    22501 atcgccaatc agaagggcgg tgtgggcaaa acgacgacga cggtaaattt ggcggcttcg
    22561 ctggcatcgc gcggcaaacg cgtgctggtg gtcgatttgg atccgcaggg caatgcgacg
    22621 acgggcagcg gcatcgacaa ggcgggtttg cagtccggcg tttatcaggt cttattgggc
    22681 gatgcggacg tgcagtcggc ggcggtacgc agcaaagagg gcggatacgc tgtgctgggt
    22741 gcgaaccgcg cgcttgccgg cgcggaaatc gagctggtgc aggaaatcgc ccgggaagtg
    22801 cgtttgaaaa acgcgctcaa ggcagtggcg gaagattacg actttatcct gatcgactgc
    22861 ccgccttcgc tgacgctgtt gacgcttaac ggcttggtgg cggcgggcgg cgtgattgtg
    22921 ccgatgttgt gcgaatatta cgcgctggaa gggatttccg atttgattgc gaccgtgcgc
    22981 aaaatccgtc aggcggtcaa tcccgatttg gacatcacgg gcatcgtgcg cacgatgtac
    23041 gacagccgca gcaggctggt tgccgaagtc agcgaacagt tgcgcagcca tttcggggat
    23101 ttgctttttg aaaccgccat cccgcgcaat atccgccttg cagaagcgcc gagccacggt
    23161 atgccggtga tggcttacga cgcgcaggca aagggtgcca aggcgtatct tgccttggcg
    23221 gacgagctgg cggcgagggt gtcggggaaa taggtcaatc caaatcgggc tgcccgtgcc
    23281 tttatgctgt ttggccgggt gcgttaatat ggcggattaa aataaaaata cttaatatcg
    23341 tcatttatag cggattaaca aaaaccggta cggcgttgcc ccgccccggc tcaaagggaa
    23401 cgattcccta agg
//
LOCUS       DAXDXW010000025        23396 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXDXW010000025 DAXDXW010000000
VERSION     DAXDXW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23396
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.025"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(1..189)
                     /locus_tag="ACFB64_000886"
                     /pseudo
     CDS             complement(1..189)
                     /locus_tag="ACFB64_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            390..6464
                     /locus_tag="ACFB64_000887"
                     /pseudo
     CDS             390..6464
                     /locus_tag="ACFB64_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            6682..8424
                     /locus_tag="ACFB64_000888"
                     /pseudo
     CDS             6682..8424
                     /locus_tag="ACFB64_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119804.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            8496..8810
                     /locus_tag="ACFB64_000889"
     CDS             8496..8810
                     /locus_tag="ACFB64_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209271.1"
                     /translation="MFGAVPDNRVKFPLAHYFKIEILYFISLLLKRCSHFFIGFIIHH
                     LIYGFWRQGLTAFAAVIDFWNRGNAFKESKAIRPDADGGAIIHLLEFVAIGADSCDDY
                     VE"
     gene            complement(9186..10226)
                     /gene="mutY"
                     /locus_tag="ACFB64_000890"
     CDS             complement(9186..10226)
                     /gene="mutY"
                     /locus_tag="ACFB64_000890"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC1209272.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VAAVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYGRARNLHKAAQQIVGQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFAFNRRETILDGNVKRVLCRVFAQDGNPQDKKF
                     ENSLWTLAESLMPSENADMPTYTQGLMDLGATVCKRTKPLCRQCPMADICEAKKQNRT
                     AELPRKKTALEVQTLPLYWLIVRNRDGAILLEKRTAKGIWGGLYCVPCFESLNGLSDF
                     AAKLSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSEHHSDGIWIKPGHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            10389..11429
                     /locus_tag="ACFB64_000891"
     CDS             10389..11429
                     /locus_tag="ACFB64_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HFC1209273.1"
                     /translation="MKAMVYHGANDIRFEEKPRPQIIDPTDAVVKIVKTTICGTDLGI
                     WKGKNPEVADGRILGHEGIGIVEEVGEAVKNIKVGDKVIISCVSKCCTCDNCKIQLYS
                     HCRNGGWILGYMIDGTQAEYVRTPYADNSLVPPHDNVNEEIALLLSDALPTAHEIGVQ
                     YGDVKPGDTVFIAGAGPVGMSALLTAQLYSPAAIIVCDMDENRLKPAKELGATHTISP
                     ASGDVSKQVFAIVGEDGVDCAIEAVGIPATWNMCQDIVKPGGHIAVVGVHGQSVDFKL
                     EKLWIKNLAITTGLVNANTTEILMKAIFSSSVDYTKMLTHRFKFSELEKAYDVFKHAA
                     ENQAMKVVLEAD"
     gene            11527..11639
                     /locus_tag="ACFB64_000892"
                     /pseudo
     CDS             11527..11639
                     /locus_tag="ACFB64_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            11650..11835
                     /locus_tag="ACFB64_000893"
     CDS             11650..11835
                     /locus_tag="ACFB64_000893"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209274.1"
                     /translation="MGYFGGNGGYLKRKQPKNLWLDVGCLKKRNFAKVSERYFLTTVN
                     LLVSAAHHGILPIGRFL"
     gene            complement(12024..12662)
                     /locus_tag="ACFB64_000894"
     CDS             complement(12024..12662)
                     /locus_tag="ACFB64_000894"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC1209275.1"
                     /translation="MSKLTPRQILTAGAVVPVMAIDDLSTAVDLSRALVEGGIPTLEI
                     TLRTPVGLEAIRLIAKEMPNAIIGAGTVTNPEQLKAVEDAGAVFAISPGLHESLARAG
                     RNSGIPLIPGVATPGEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(12872..14707)
                     /gene="edd"
                     /locus_tag="ACFB64_000895"
     CDS             complement(12872..14707)
                     /gene="edd"
                     /locus_tag="ACFB64_000895"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC1209276.1"
                     /translation="MNHTPIHPKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVVKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFICVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNVREVERIDLGANQQGCGRELFAGFRSMTSSAETGAMSFGGEFA"
     gene            complement(14791..14909)
                     /locus_tag="ACFB64_000896"
                     /pseudo
     CDS             complement(14791..14909)
                     /locus_tag="ACFB64_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            15227..16672
                     /gene="zwf"
                     /locus_tag="ACFB64_000897"
     CDS             15227..16672
                     /gene="zwf"
                     /locus_tag="ACFB64_000897"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC1209277.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSGEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTIE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFRPLQNHIFDNSQTAPNRLVIELQPNESVRLYTQVKTPGAGNKVEV
                     TPLGVDLGKAVEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWANNT
                     TPPHGYGAHSWGPEAARELLARDGNKWHEEQ"
     gene            16823..17518
                     /gene="pgl"
                     /locus_tag="ACFB64_000898"
     CDS             16823..17518
                     /gene="pgl"
                     /locus_tag="ACFB64_000898"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC1209278.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTNHADSNTGLVREYLLKNKAAAAVWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLILGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERISMTLDAIAHTGHVFLAIQGEEKKAVFDQAAQGENREYPISLVL
                     NHQGVNCHVFYAE"
     gene            17499..18485
                     /locus_tag="ACFB64_000899"
     CDS             17499..18485
                     /locus_tag="ACFB64_000899"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC1209279.1"
                     /translation="MSSTPNKHADYPRLVADIGGTNARFALETAPCVIEKVAVLPCKE
                     YDTVTDAVRAYLNQSGATGVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQALGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSPAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYRHVSAERFLSGAGLSLIYETLAAKQKAEP
                     AKLMPSEITEKALNCESPLCRQALDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRM
                     LDYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGIAGAAAALGNHLKNV"
     gene            18534..19382
                     /gene="hexR"
                     /locus_tag="ACFB64_000900"
     CDS             18534..19382
                     /gene="hexR"
                     /locus_tag="ACFB64_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC1209280.1"
                     /translation="MLSKISESLADLSGAERKVAECALAEPKWFVHAAVAEIADRASV
                     SQPTVIRFCRSLGYKGLPEFKLSLSASIGHEGMPYVHEELNADDDMANVVEKVLGNAA
                     AALLGERRFLKESELENGIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            19608..21254
                     /gene="pgi"
                     /locus_tag="ACFB64_000901"
     CDS             19608..21254
                     /gene="pgi"
                     /locus_tag="ACFB64_000901"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC1209281.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIMVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     TMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNRKAVAEFGIDIANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDRHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMCGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETEPQKHDSSTERLINLYLQTNRK"
     gene            22032..22484
                     /locus_tag="ACFB64_000902"
     CDS             22032..22484
                     /locus_tag="ACFB64_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFC1209282.1"
                     /translation="MALIKEPLDKAKQRNEKLEAAEATAQEAREAEEAAAQEALGREQ
                     EAARVSEWEERYKLSRSEFEQFWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGE
                     TPNGIKVSELACKTAETEARLEELHNRKKALIDEMVREEDKKELPKRL"
     gene            22529..22669
                     /locus_tag="ACFB64_000903"
     CDS             22529..22669
                     /locus_tag="ACFB64_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209283.1"
                     /translation="MPEYRNWQNGLGAKCADGDEYGVAQSDCRTREINAKTKEIQGYL
                     ID"
     gene            22774..23157
                     /locus_tag="ACFB64_000904"
     CDS             22774..23157
                     /locus_tag="ACFB64_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFC1209284.1"
                     /translation="MIYYPAVLFQDPDQSNWGVIIPDLPGCYPVGDTVADALADAKAA
                     AMFHLEGLLQENLPIPEAQSIEVNRDNPDYADAVLWTMVEADDAALTGQVRFNVSWPQ
                     HILNRVDACTAARHETRAVFWRKPP"
     gene            complement(23241..23375)
                     /locus_tag="ACFB64_000905"
                     /pseudo
     CDS             complement(23241..23375)
                     /locus_tag="ACFB64_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
BASE COUNT         6235 a         5808 c         6044 g         5309 t
ORIGIN      
        1 tttacctttg cgttgtccgc caaaataact ttcatctgct tctacttcgc catcaaacat
       61 ttctaaatgc ggaccgtttt gataaatcag tcatcgtaaa cgatgaaaat cataggctgc
      121 ggtattttta ttaacgccta ccaactctgc cgctgttctt gcagttacgc ctgcgacaaa
      181 tagcccaatg agtttatttt gtttatgccg gcttagacgg cttttctcat tggcttattc
      241 taacctaaat gggattttta gccgttatcc ggtacgtccc ctaatttaac aaaaaaatgg
      301 cggtaattga cttaccaaaa tctttgtcct agaatgccca gactgtccaa tgtttgtaat
      361 aactctataa aaatcaaata actaaaatca tgaacaaaac cctctatcgt gtgattttca
      421 accgcaaacg cggtgctgtg gtagctgttg ccgaaaccac caagcgcgaa ggtaaaagct
      481 gtgccgatag tggttcgggc agcgtttatg tgaaatccgt ttctttcatt cctactcatt
      541 ccaaagcctt ttgtttttct gcattaggct tttctttatg tttggctttg ggtacggtca
      601 atattgcttt tgctgacggc attattactg ataaagctgc tcctaaaacc caacaagcca
      661 cgattctgca aacaggtaac ggcataccgc aagtcaatat tcaaacccct acttcggcag
      721 gggtttctgt taatcaatat gcccagtttg atgtgggtaa tcgcggggcg attttaaaca
      781 acagtcgcag caacacccaa acacagctag gcggttggat tcaaggcaat ccttggttga
      841 caaggggcga agcacgtgtg gttgtaaacc aaatcaacag cagccatcct tcacaactga
      901 atggctatat tgaagtgggt ggacgacgtg cagaagtcgt tattgccaat ccggcaggga
      961 ttgcagtcaa tggtggtggt tttatcaatg cttcccgtgc cactttgacg acaggccaac
     1021 cgcaatatca agcaggagac tttagcggct ttaagataag gcaaggcaat gctgtaatcg
     1081 ccggacacgg tttggatgcc cgtgataccg atttcacacg tattcttgta tgccaacaaa
     1141 atcaccttga tcagtacggc cgaacaagca ggcattcgta atcaagggca gttgtttgct
     1201 tcttccggta atgtggcgat tgatgcaaat ggccgtttgg tcaatagtgg cacgatggct
     1261 gccgccaatg tgcaagatat gaataataca gcggaacaca aagtcaatat ccgcagtcaa
     1321 gcctttgaaa acagcggtac ggcggtatcg caacaaggca ctcaaattca cagtcaatcg
     1381 attcaaaaca ctggcaaatt attgtcggca ggaacagagg atttagccgt ttcaggcagc
     1441 ctgaacaatc aaaatggcga aatagcgacc aatcaacaac tgattattca cgatggtcag
     1501 caatctaccg ttgtcattga taatacgaat ggcacgatac aatcaggccg tgatgttgcc
     1561 attcaggcaa aatcgttatc caacaacggc acacttgccg ctgataataa actggatatt
     1621 gcgttacaag atgattttta tgtagaacgc aagatcgtgg cgggcaatga attgtcgctc
     1681 agtacacgag gcagcctgaa aaattcacat accttgcaag caggaaaacg cattcggatt
     1741 aaagcaaata accttgataa tgcagtacaa ggcaacattc aatccggcgg tacgacagac
     1801 attggcacgc agcacaattt aaccaataga ggcttgattg acggacaaca aaccaaaatc
     1861 caagccgggc aaatgaataa tatcggtaca ggtcggattt atggcgacaa tatcgctatt
     1921 gcggctaccc gcttagacaa tcaagatgaa aacggtacag gtgccgccat tgcggcacgc
     1981 gaaaacctga atttaggcat tgaacaatta aataaccgtg aaaacagtct gatttacagc
     2041 ggtaacgata tggcggttgg cggcgcatta gataccaatg accaagccac aggcaaagcc
     2101 caaaggatac acaatgccgg cgcaatcatt gaagctgcag gcaaaatgcg tttaggtgta
     2161 gaaaagctgc acaataccaa tgaacatttg aaaacgcagt tggtagaaac agggcgcgag
     2221 cgtattgttg attacgaagc atttggacga cacgaattat tgcgagaagg cacgcaacat
     2281 gaattaggct ggtttgtcta caacaatgaa tcagaccact tacgcacccc tgatggagtg
     2341 gcgcatgaaa attggcataa atacgattat gaaaaagtaa cgcaagaaac tcaagtaacc
     2401 ggaactgcgc ctgctaaaat cattgcaggt agcgatttga ttattgatag caaagcagtc
     2461 ttcaacagcg acagccgaat catcgccggc ggcaatttgc ttgtgcaaac agaaaaagac
     2521 ggtttgcata acgagcaaac ctttggcgag aagaaagtct tcagcgaaaa tggtaagttg
     2581 cacaactact ggcgtgcgcg tcgtaaagga catgatgaaa cagggcatcg tgaacaaaat
     2641 tatactttgc cggaggaaat cacacgcgac atttcactgg gttcatttgc ctatgaatcg
     2701 catagcaaag cattaagccg tcatgcgccc agccaaggca ctgagttgcc acaaagtaac
     2761 cgggataata tccgtactgc gaaaagcaac ggtatttcgc taccctatac gcccaattct
     2821 tttaccccat tacccggcag cagcttatac attatcaatc ctgccaataa aggctatctt
     2881 gttgaaaccg atccacgctt tgccaactac cgtcaatggt tgggtagtga ctatatgctg
     2941 ggcagcctca aactagaccc aaacaattta cataaacgtt tgggtgatgg ttattacgag
     3001 caacgtttaa tcaatgaaca aatcgcagag ctgacagggc atcgtcgttt agacggttat
     3061 caaaacgacg aagaacaatt taaagcctta atggataatg gcgcgactgc ggcacgttcg
     3121 atgaatctca gcgttggcat tgcattaagt gccgagcaag cagcgcaact gaccagcgat
     3181 attgtttggt tggtacaaaa agaagttaaa cttcctgatg gcggcacaca aaccgtattg
     3241 atgccacagg tttatgtacg cgttaaaaat ggcggcatag acggtaaagg tgcattgttg
     3301 tcaggcagca atacacaaat caatgtttca ggcagcctga aaaactcagg cacgattgca
     3361 gggcgcaatg cgcttattat caataccgat acgctagaca atatcggtgg gcgtattcat
     3421 gcgcaaaaat cagcggttac ggccacacaa gacatcaata atattggcgg cattctttct
     3481 gccgaacaga cattattgct caatgcgggt aacaacatca acaaccaaag cacggccaag
     3541 agcagtcaaa atgcacaagg tagcagcacc tacctagacc gaatggcagg tatttatatc
     3601 acaggcaaag aaaaaggtgt tttagcagcg caggcaggca aagacatcaa catcattgcc
     3661 ggtcaaatca gcaatcaatc agatcaaggg caaacccggc tgcaggcagg acgcgacatt
     3721 aacctggata cggtacaaac cggcaaatat caagaaatcc attttgatgc cgataaccat
     3781 accatccgag gttcaacgaa cgaagtcggc agcagcattc aaacaaaagg cgatgttacc
     3841 ctattgtcag ggaataatct caatgccaaa gctgccgaag tcggcagcgc aaaaggcaca
     3901 cttgccgtgt atgctaaaaa tgacattact atcagctcag gcatccatgc cggccaagtt
     3961 gatgatgcgt ccaaacatac aggcagaagc ggcggcggta ataaattagt cattaccgat
     4021 aaagcccaaa gtcatcacga aactgctcaa agcagcacct ttgaaggcaa gcaagttgta
     4081 ttgcaggcag gaaacgatgc caacatcctt ggcagtaatg ttatttccga taatggcacc
     4141 cggattcaag caggcaatca tgttcgcatt ggtacaaccc aaactcaaag ccaaagcgaa
     4201 acctatcatc aaacccaaaa atcaggattg atgagtgcag gtatcggctt cactattggc
     4261 agcaagacaa acacacaaga aaaccaatcc caaagcaacg aacatacagg cagtaccgta
     4321 ggcagcctga aaggcgatac caccattgtt gcaagcaaac actacgaaca aaccggcagc
     4381 aacgtttcca gccctgaggg caacaacctt atcagcacgc aaagtatgga tattggcgca
     4441 gcacaaaacc aattaaacag caaaaccacc caaacctacg aacaaaaagg cttaacggtg
     4501 gcattcagtt cgcccgttac cgatttggca caacaagcga ttgccgtagc acacaaagca
     4561 gcaaacaagt cggacaaagc aaaaacgacc gcgttaatgc catggcggct gccaatgcag
     4621 gttggcaggc ctatcaaaca ggcaaaggcg cacaaaactt agccaatggt acaaccaatg
     4681 ccaaacaagt cagcatctcc ataacctacg gcgaacagca aaaccgacaa accacccaag
     4741 ttcaagccaa tcaagcccaa gcgagtcaaa ttcaagcagg cggcaaaact acccttattg
     4801 ccgaaggtgc ggcgaacaat ccaatatcaa catcacaggc tcaggtgttt caggcagagc
     4861 aggaaccggc ctgattgccg ataagcaaat ccatctgcaa tcagccgagc aaagcaatac
     4921 cgaacgcagc caaaacaaat cagcaggctg gaacgcaggt gctgccgtat cattcggaca
     4981 aggaggctgg tcattaggcg ttgccgcagg cggcaatgtc ggcaaaggct acggcaatgg
     5041 cgatagcgta acccaccgcc atagccatat tggcgacaaa ggcagccaaa cccttatcca
     5101 aagtggtggc gataccatca tcaaaggcgc gcaagtacgc ggcaaaggcg tacaagtcaa
     5161 tgccaaaaac ctaagcattc aaagtgtaca agatagagaa acttatcaaa gcaaacaaca
     5221 aaacgccggt gcacaagtta ccgtaggtta tggcttcagt gccagtggcg attacagcca
     5281 aagcaaaatc cgagccgacc atgcttcggt aaccgagcaa agcggtattt atgccggaga
     5341 agacggctat caaatcaagg tcggaaacca tacaggcctc aaaggcggca tcatcaccag
     5401 cagccaaagc gcaaaagaca agggtaaaaa ccgattcagc acaggcacac tcgccggcag
     5461 tgatattcaa aattacagcc aatacgaagg aaaaagtttt ggattgggtg ccagcgttgc
     5521 cgtaagcggc aaaacactgg gacagggcgc aaaaaataaa cctcaagaca aacacctgac
     5581 aagcgtagcc gataaaaacg gcgcaagttc atcagtaggg tacggcagcg acagcgacag
     5641 tcaaagcagc atcacaaaaa gcggcatcaa tacccaaaaa cattcaaatc acagacgaag
     5701 ccgcacaaat caggctgaca ggcaaaatag cggcacaaac caaagccgat attgatacaa
     5761 acgtaaccac agacaccgcc gaacgacatt cgggcagcct gaaaaacata tttgacaaag
     5821 atagagtgca aagtgaactg gatttacaaa gaaccgtcag ccaagatttt agtaaaaatg
     5881 ttcaacaaac caataccgag attaaccaac atttagacaa actcaaagca gacaaagaag
     5941 cagccgaaac agcagcagcc gaggcattag ccaatggcga tatggaaact gccaaacgca
     6001 aagcccatga agctcaagat gcggcagcaa aagcagataa ttggcaacaa ggcaaagtca
     6061 ttctcaacat gttagcctca ggtttagctg agccgaccca aagcggagcg ggcatcgctg
     6121 cggctaccgc atcgccagac gtatcgtatg cgattggaca gcactttaaa gatttagccg
     6181 gtcaaaacgc gaatggcaaa ctaaccgcca gtcaagaaac cgctcacgtt cttgcccacg
     6241 cggtattagg agcagcggtt gccgcagcat gaggcaacaa tgccccggca ggagcattgg
     6301 gtgcgggcgg gtcggaagcg gccgccccaa tcatcggcaa atggctgtac ggcaaaggag
     6361 acggcggcag cctgaatgcg gaggaaaaag agaccgtttc ggcgattaca aggatgctgg
     6421 gtacggctgc cggagcagct gagggaaact cgtccgccga tgctgtgtgg ggttgttttc
     6481 agacggcttc agatttcgct tcctcttttt catatcctat aaacatgtga ttaaattttc
     6541 atttatggag tttttttatc tttttgtttg tttttgtgca ttgctataat tgcccgctta
     6601 cattacttag accaatccta tgaaaccttt cttttcctcc aaaaatgtat ttcatcccta
     6661 tcccttatca ctgttagtcc tatcattgat ggctgcctcc ccaccctaac atcagccgcc
     6721 cccacacctg aagctgtttc acaacaacag gatattctcc aacgtcaacg tgagaagcaa
     6781 ttgcgtgaac agatgcagcc ggaacaagat gttcgtttgg atggtaccga tacgggcata
     6841 gagaagatgg caacacaggt aggcggtgca aattcggatg aggcaagccc ctgctttcct
     6901 atttctgagg tggaattggt gggtgaagaa gcggctaaat tccggtttgc gctcaaccat
     6961 gccttgtgcc aaacacattt tgtttccggc aagtgtctgc atgcgggcga cattaatcaa
     7021 atcatgtcct tagcacaaaa tgctttgatc ggcaggggat ataccacgac ccgtatcttg
     7081 gctgcgccac aggatttgaa tagtggcaag cttcaattaa ccctgatgcc gggctatctg
     7141 cgctccatac gaatcgatcg gtccaacgat gatcaaaccc atgcaggacg tattgcagca
     7201 ttccaaaaca aatttcccac ccgctcgaac gatctgttga atctgcgtga tttggaacaa
     7261 ggactggaaa atctcaaatg tctcccgact gcggaagccg atctccaaat cgttcccgta
     7321 gagagagaac caaaccaaag tgatgtcgtg gtgcaatggc ggtaacgtct gctgccctac
     7381 tgtgtgagtg tggggatgga taattcgggt agtgaggcga caggaaaata ccaaggaaat
     7441 atcactttct ctgccgacaa tccttttgga ctgagtgata tgttctatgt aaattatgga
     7501 cgttcaattg gcggtacgcc cgatgaggaa aattttgacg gccatcgcaa agaaggcgga
     7561 tcaaacaatt acgccgtaca ttattcagcc cctttcggta aatggacatg ggcattcaat
     7621 cacaatggct accgttacca tcaggcggtt tccggattat cggaagtcta tgactataat
     7681 ggaaaaagtt acaacactga tttcggcttc aaccgcctgt tgtatcgtga tgccaaacgc
     7741 aaaacctatc tcagtgtaaa actgtggacg agggaaacaa aaagttacat tgatgatgcc
     7801 gaactgactg tacaacggcg taaaaccaca ggttggttgg cagaactttc ccacaaagga
     7861 tatatcggtc gcagtacggc agattttaag ttgaaatata aacacggcac cggcatgaaa
     7921 gatgctctgc gcgcgcctga agaagccttt ggcgaaggca cgtcacgtat gaaaatttgg
     7981 acggcatcgg ctgatgtaaa tactcctttt caaatcggta aacagctatt tgcctatgac
     8041 acatccgttc atgcacaatg gaacaaaacc ccgctaacat cgcaagacaa actggctatc
     8101 ggcggacacc acaccgtacg tggcttcgac ggtgaaatga gtttgcctgc cgagcgggga
     8161 tggtattggc gcaacgattt gagctggcaa tttaaaccag gccatcagct ttatcttggg
     8221 gctgatgtag gacatgtttc aggacaatcc gccaaatggt tatcgggcca aactctagcc
     8281 ggcacagcaa ttgggatacg cgggcagata aagcttggcg gcaacctgca ttacgatata
     8341 tttaccggcc gtgcattgaa aaagcccgaa tattttcaga cgaagaaatg ggtaacgggg
     8401 tttcaggtgg gttattcgtt ttgatgcggt tgaagtgaat aatgtatgac aagatacaca
     8461 aaagctgccg gttttttgca ggcagctttt gttttatgtt tggggccgta ccggacaacc
     8521 gggtcaaatt tccattagct cattatttta aaattgaaat tttatatttt atttcactgt
     8581 tgttaaaacg ctgttcacat ttcttcatcg gttttatcat tcatcacctt atttacggct
     8641 tctggagaca ggggcttacc gcatttgctg cagtaatcga tttttggaac aggggaaatg
     8701 cctttaaaga aagtaaagct atccgacccg atgccgacgg aggtgccata attcatcttt
     8761 tggaattcgt ggcaataggg gcagattcct gcgatgatta tgtagaataa aattatgata
     8821 cctccgaaac tgaaatatat cacaaatatt atttacataa ttgttatttg cagaaaatta
     8881 gaacccgtta tcacgtcttg gataaatgcc aaaaccactg tgattccgag aattaaaaag
     8941 aaaaatttcg gaatcctcat ccttttctga aacctgattg cggtatccat ggctttgtct
     9001 ttgtcattta agtttgtgtt catgtttttg accctctttg tttctaaaat cctttttaaa
     9061 ttgccgactt tttccgttcc aaatgtccca tttgaacatt atcccgctgc ctaaaatacg
     9121 acccaaattc atcggttgtt cttgctgcga tacctgctaa caacagttca acgggtttat
     9181 tttgtttatt ctaacctatt accgtttaaa taaatttcca aaggcttggg caggccgtaa
     9241 tctttcaaat ggcccggctt tatccaaatg ccgtctgaat ggtgttcgga cggcatttgc
     9301 ccttcaaacg gcgtaatcat cagcagccgg tgcgtcaggc ggtgggtcag ggcggtttgt
     9361 tcgtccatat ccgccatcgt cagggagagt ttggcggcaa agtcggaaag cccgttcaaa
     9421 ctttcaaaac acggcacgca atacagtccg ccccaaatgc ctttggcggt gcgtttttcc
     9481 agcaagattg cgccgtcccg gttgcggaca atcagccagt aaagcggcag ggtttgcact
     9541 tcgagggcag tttttttacg cggcagctcg gcggtgcggt tttgcttttt tgcttcgcag
     9601 atgtccgcca tagggcattg acggcacaag ggtttcgtcc gtttgcacac ggtcgcgccc
     9661 aaatccatca aaccttgcgt gtaagtaggc atatcggcgt tttcagacgg catcaggctt
     9721 tcggcaagtg tccagagcga gttttcaaat tttttatctt gcggattgcc gtcttgggca
     9781 aaaacacggc agagaacgcg tttgacgttg ccgtccaaga tggtttctcg tcggttgaaa
     9841 gcaaaggcag aaatggcggc ggcggtgctt ctgcctacgc cgcagagcgt ttccaagtct
     9901 ttgcgctccg atggaaacgt accgccgaat tgtccgacga tttgttgcgc ggctttgtgc
     9961 agattgcgcg cgcggccgta atagcccaag cccgcccaca acgacaacac ttcgtcttgc
    10021 ggcgcggcgg caagcgtctg aacggtcggg aatttttcca agaaacgcgg atagtagtcc
    10081 aacacggcgg cgacctgcgt ttgctggagc atgatttcgg aaagccagac gcaataaggg
    10141 tttttgacct gccaagggag gtggtggcga ccgtgttgtt tttgccagcg gatgagccgt
    10201 tcggagaagg ggatgggtgt gttcattaat atcaatcggt ggttttattt atatttaaaa
    10261 cagtatgtta ttgtataaaa ttgtgaaaat aattcttatt gacttatttt ttgtaggggc
    10321 atataactca tataaagaaa ctttgttgcg gtattgaaat tatttatcaa caagcaagga
    10381 gtatcagcat gaaagcaatg gtttatcacg gcgcaaacga catccgtttt gaagaaaaac
    10441 cccgcccgca gattatcgat ccgaccgatg cggtggtgaa aatcgtcaaa accacgattt
    10501 gcggtaccga cttgggtatt tggaaaggca aaaaccccga agtcgccgac ggtcgtatcc
    10561 tcggtcatga aggcatcggt attgtagaag aagtcggcga ggctgtaaaa aacatcaaag
    10621 tcggcgataa agtcattatt tcatgcgtca gcaaatgctg cacttgcgac aactgcaaaa
    10681 tccaacttta ttcgcactgc cgcaacggcg gctggatttt gggctatatg attgacggca
    10741 cgcaggccga atacgtccgc acgccttatg ccgacaacag cctcgtgccg ccgcacgaca
    10801 acgtcaacga agaaatcgcc ctgctgttga gcgacgcttt accgaccgcc cacgaaatcg
    10861 gcgtgcaata cggcgatgtg aaacccggcg ataccgtatt catcgcaggt gccggccctg
    10921 tcggtatgtc cgccttgttg accgcccaac tgtatagccc tgccgccatt atcgtgtgcg
    10981 atatggacga aaaccgtttg aaaccggcga aagagttggg cgcgacccat accatcagcc
    11041 ccgcttccgg cgacgtctcc aaacaagttt tcgccatcgt cggcgaagac ggcgtagatt
    11101 gtgccatcga agcggtcggt atccctgcaa cttggaatat gtgccaagac atcgtgaaac
    11161 ccggcggtca tatcgccgtc gtcggcgtac acggtcaatc cgttgatttc aaactggaaa
    11221 aactgtggat taaaaatctt gccatcacta ccggtttggt caatgccaac accaccgaaa
    11281 tactgatgaa ggcaattttc agcagctcag tggattacac caaaatgttg acccaccgtt
    11341 tcaaattcag cgaattggaa aaagcctacg acgtgttcaa acacgccgcc gaaaaccaag
    11401 cgatgaaagt ggttttggaa gcggattaat cgccgttgaa tcagttgttt tttaagcgga
    11461 tgccgtctga acgtgtttca gacggcattt tttcagccgt gtaaaacggg ctgttcctga
    11521 tcaatcaggt atagtggatt aaatttaaat caggacaagg caacgaagcc gcagacagta
    11581 aaatagtacg gcaaggcgag gtaacgccgt actggtttaa atttaatcca ctatatttaa
    11641 gggggattaa tgggatattt tggtgggaac ggcggttatt tgaaacgaaa acagccgaaa
    11701 aacctgtggt tggatgtcgg ctgtttgaag aaaaggaatt ttgcaaaggt ctctgagcgt
    11761 tattttttaa cgacggttaa cttgttagtt tcagccgctc atcacggcat tttaccgata
    11821 gggcgatttt tatgactaat tgataaatat attataatta ttttttcgtc ataggtctcc
    11881 gcatatcgcg cgtcattcgc attgttctgg agatggatgg gcattttagg ggcggtgctg
    11941 ctgtgtatgc cgactgagtc gccaagcggc aaaatgccgt ctgagactgg aaagggcttc
    12001 agacggcatt tttacgatgc ggttcaggct ttgggtttca atgccgccgc ttctttggcg
    12061 aggcgggtaa tggtgtccca gtctttgttt tttacagctt ctttcggtgt cagccaagag
    12121 ccgccgacgc ataagacgtt gggcaatgcc aagtaatcgg gcgcagtggc gaggctgatg
    12181 ccgccggtcg ggcagaagcg tacgtcggcg taaggtccgt agagggcttt gagcatcgct
    12241 ttgccgccaa cgacttcggc agggaagagt ttgagtgtgt cgatgccgtg ttccaaagcc
    12301 agttggactt cgcccggagt ggcaacgccg ggaatcaggg gaatgccgct gttgcggccg
    12361 gctctggcga gggattcgtg caaacccggg ctgatggcga aaaccgcgcc tgcgtcttcg
    12421 acggctttga gctgttcggg attggttacc gtacctgcgc cgatgatggc gttgggcatt
    12481 tctttggcaa tcaggcggat ggcctcgagt ccgacggggg tgcgcaaggt aatttcgagg
    12541 gtggggatgc cgccttcgac aagggcgcgg gacaaatcga cggcggtgct taagtcgtca
    12601 atcgccatca caggcacaac tgcgccggcg gtcaggattt ggcggggggt cagtttggac
    12661 atttcggttc tccaagtggg gggtgtcgtt tttgttgagg tcaaacggga tggggggggt
    12721 taagttttca gacaaccttt ggggtaaaac attctgatgt tgcttgggtt ttgttcgcgg
    12781 gcaaagctca cgctacgtct gcatctattt taaacggctt caataatttt tcagacggcc
    12841 ttctgtttta aaaggccgtc tgaaacgcgc atcaggcaaa ttctccgccg aagctcatcg
    12901 caccggtttc cgcactgctg gtcatactgc ggaagccggc gaagagttcg cggccgcagc
    12961 cttgttggtt cgcgcccaaa tcgatgcgct cgacttctcg gacgttccat tcggcctcgt
    13021 taatcaggac gttgagttcg ccggtaacgg agtcgaagcg aatcaggtcg ccggtacgga
    13081 ttttggcgat gttgccgccc atcagggctt cgggcgtcat gtggatggac gcgggcactt
    13141 tgcctgacgc gccggacata cggccgtcgg tcagcagcgc cactttgaag ccgcggtctt
    13201 gcaggatgcc caaaggcggg gtcagtttgt gcaattcggg cataccgttg gcacgcgggc
    13261 cttggtagcg gacgacgcag ataaaatcgc gttccaactc gccgcgttca aatgccgcca
    13321 acacttcgcg ctggtcgttg aacacgatgg cgggtgcttc gatgatgcgg cagccttcgc
    13381 gtacggctga cactttaacc acgccgcgtc cgatgttgcc tttcatcagg cgcagaccgc
    13441 cgtccgggga gaacggattg tcggctttac gcaggatgtc gtcgttgccg ctggtctcgg
    13501 gggcttcgcg ccattcgagt ttgccgtcga tgaggaaagg ctctttagtg tagtggcgca
    13561 taccgtgtcc gacgacggta tcgacatcgt cgtgcaataa gcctgcgtcc agcaattcgc
    13621 ggataacgaa aggcaggccg cctgctgcgg taaagtggtt cacgtcggct ttgccgttgg
    13681 gatacacgcg gatgagcagc gggatgatgg aagaaatttc gtcgaagtcg tcccagttca
    13741 aaatcacgcc tgcggcgcgc gccatagcga cgaggtgcat ggtgtggttg gtcgagccgc
    13801 cggtcgccat caaaccgatc agggcattga tgaaggattt ttcggtcaac atttcgccca
    13861 aaggtttaat cgtgccgttt ttgatgccgc gcgcgaggtg tccggcggcg tagcgggtca
    13921 gggcttcgcg caggtcggta taggggtgga caaaggcggc ggcaggcagg tgtacgccca
    13981 tcatttccat catcatttgg ttggagtttg ccgtgccgta gaaagtgcag gtgcccgggc
    14041 tgtggtaaga acccatttcg cttttcaaaa gttcgtcgcg tccgactttg ccttcggcga
    14101 aaagttggcg ggtacgggct ttttctttat tgccgatgcc gctggacatc gggcctgccg
    14161 gaacgaagat accgggaata tgaccgaacg aaagcgcacc tatcatcaga cccggaacga
    14221 ttttgtcgca cacgcccata aacaggctgc cgtcaaacat ttggtgcgac agcccgacgg
    14281 cggtactcat cgcaatcacg tcgcgggaaa acagcgacaa ttccatgccg gcgtagcctt
    14341 gcgtgatgcc gtcgcacatg gcaggcgtgc cgccggcgac ttgtgcggtc gcgccgtttt
    14401 tttgtgcttc gtctttgatt tggtcaggga agtctttaaa cggctggtgt gcggaaacca
    14461 tgtcgttgta ggcagtgatg atgcctaagt tggggacggt ttcctgaagc atttcgattt
    14521 tgatgctttt gggcatggcg gcataaccgt gcgccaaatt gctgcagccg agctggttgc
    14581 gctctaagcg tcccatttgt ttggcactgc ggattttcgc cagatatttt tcacgcgtcg
    14641 gacggctgcg ttcgataatg cgcccggtga tttcggcgag tttggggtgg ataggagtgt
    14701 ggttcatgtt cggtctcctg gcggaacggg gtggtattgg ggatttgttt gtgcgtttct
    14761 gatttttgta tcgggtatgt tagacggtga gtatagtgga ttaaatttaa accggtccgg
    14821 cgttgcctcg ccttgccgta ctatttgtgc tgtctgcggc tcgccgcctt gtcctgattt
    14881 aaatttaatt cactatattt taaaaggtac gccgtctgaa agtgggaagc gggtgctatt
    14941 atagtacaaa atttgtaatt ttgttacaaa aaaatcagac attgaaaaga aatttgattt
    15001 aaatcgcaga atcttctgat tgttgtaacg gaatagggga aacctgcctg atttttggac
    15061 taggtcaaat aaattgtaaa aatgggaata gacaaaaagt gtagcggtgt gtagtattac
    15121 tactcattat gccgcctgcc tttgtttccg atattgcgat gctgcgtgat ggaaaaaaca
    15181 ggtttcagac ggcatcgaaa aaatcctttc ctgacgagat aatgcgatga gtacacagac
    15241 aaattttgat ttggtgttgt tcggtgcgac cggcgatttg gcaatgcgca aacttttgcc
    15301 ctgcctgtat caggcgcatg ttgccggtct gcttcatccc gaagggcgta ttttgggggt
    15361 cagccgcagc gagttggata ccgaaggttt tctggcgaag gtggaaacca gttccaaaat
    15421 ccatgtgaaa gaaaattttt ccggtgaggc gtgggcttcg tttgttgaac gtctcgcata
    15481 tctcaaagtc gatgttacgc agccggacga ttttgccgct ttgggcgatt tggtcaaagc
    15541 acgcaaagaa accgacaacg tcgtcatcta tctgtccacc gcgccgaaat tcttcgcgca
    15601 agcctgcgaa aaccttgctg cgatcggttt gaatgccgac aatgtgcgcg tggttttgga
    15661 aaaaccgctg ggtacggatt tggcttcttc ccaacaaatc aataccgatg tggcgcgtta
    15721 tttcaaagaa gggcaaattt accgcatcga ccactacctt ggcaaagaaa gcctgcaaaa
    15781 cctgcttgcg ctgcgttttg ccaatgtaat gttcgagccg ctgtggaaca ataaatacat
    15841 cgaaagcgtg cagctgacca tcgccgaaca gctcggtgtg gaagagcgcg gcgagtttta
    15901 cgacattacc ggcgcgttgc gcgatatggt gcaaaaccat ttgatgcaga tgctgtgcat
    15961 gactgcgatg gaagcccccg ccagcttgga tgccgacgcg gtgcgcgatg aaaaagtcaa
    16021 agtcatcaag tcattgaagc cgctgaccat cgaatctgtc aatgaaaatg tcgtgcgcgg
    16081 acaatatacc gctgccaaag gcatgaacgg ctatcttgaa gaaatcaacg ttccgcaaga
    16141 cagctttacc gaaacctacg tcgccattaa agccgaaatc gaaaacgaac gctggaaggg
    16201 cgttcccttc tacctgcgta ccggcaaacg catggcgggc aaagtggcgg aaatcgtttt
    16261 gaacttccgc ccgctgcaaa accatatttt cgacaacagc caaaccgcgc cgaaccgttt
    16321 ggttatcgag cttcaaccga atgaatccgt ccgcctttac acgcaggtga aaaccccggg
    16381 tgcgggcaac aaagtggaag taacgccttt gggcgtggat ttgggcaagg ccgttgaagg
    16441 ccgccgcgcc gaggcttacg aacgcctgct gttggatgtg attaacggca aactcgcttt
    16501 gttcaaccgc cgcgacgaac tcgaagccgc gtgggaatat gtgatgccga ttttggaaaa
    16561 ctgggcaaat aacaccacgc cgccgcacgg ttacggcgca cactcttggg gccctgaagc
    16621 cgcccgcgaa ctgttggcgc gcgacggcaa caagtggcac gaagaacagt aatgcgcttc
    16681 agacggcatc gggttcgcaa tgccgtctga aggagggcgg gcagaaaaag cagtcccgct
    16741 tcgaggcgga agcggctaag ccattgcccg gcagccgccc tgtaacggga tatgccgtct
    16801 gaaatcacaa aaggacacaa atatgtttgt ttggcacgaa tacgaaaatg cggcagaagc
    16861 ggcgcagtct ttggctgacg cagtggcgga tgctttgcag ggcgcactgg acgagaaggg
    16921 cggtgcggtg ttggcagttt ccggcggacg ttcgccgatt gcatttttca acgccctgtc
    16981 gcaaaaagat ttggattgga aaaacgtcgg aattaccttg gcagatgaac gcatcgtgcc
    17041 gaccaaccac gccgacagca ataccggtct ggtgcgcgaa tacctgttaa agaacaaagc
    17101 cgctgcggca gtgtggattc ctatggtgga agacggaaaa actgaaaccg aattacatcc
    17161 cgatgctgtt gtcgattatg cgctgaaaca ttacaagcag cccgatgttt tgattttggg
    17221 tatgggaaac gacgggcata cggcttcgat tttcccaaaa gctccgcagt ttcagacggc
    17281 aatcgacggt tcggcaggtg tcgcgctggt gcataccacg cccgttaccg cgccgcacga
    17341 gcgcatcagc atgactttgg atgcgattgc ccatactggg catgtatttt tggcgataca
    17401 gggcgaagag aaaaaagccg tgttcgacca agccgcacaa ggcgaaaacc gcgaatatcc
    17461 gatcagcctc gttttgaacc atcaaggagt gaactgccat gtcttctacg ccgaataaac
    17521 acgccgatta cccgaggctg gttgccgaca tcggcggcac aaacgcccgt ttcgcattgg
    17581 aaaccgcgcc gtgcgtgatt gagaaagtcg ccgtcctgcc gtgtaaggaa tacgacacgg
    17641 ttaccgacgc ggtgcgtgcc tatctgaacc aaagtggtgc aacgggcgta cggcacgcgg
    17701 catttgccat cgccaacccg attttgggcg actgggtgca gatgaccaac caccattggg
    17761 cgttttccat cgaaaccacc cgtcaggctt tggggctgga caccctcatc cttttgaacg
    17821 actttaccgc gcaggcattg gcggtaacgc agacttcaag caaagacctg atgcaggtag
    17881 gcgggcaaaa gcctgtcgaa ttcgccccca aggccgttat cggccccggt accggcttgg
    17941 gcgtgagcgg attggtgcac agccccgcag gctgggtggc gttggcgggc gagggcgggc
    18001 ataccagttt cccgccgttt gacgatatgg aagtgttgat ttggcagtac gccaaaaaca
    18061 aataccgcca cgtttccgcc gaacgtttcc tcagcggcgc aggtttgagc ctgatttatg
    18121 aaacgctggc ggccaagcag aaagccgaac cggcaaaact gatgccgtct gaaatcaccg
    18181 aaaaggcatt aaattgcgaa tcgcccctgt gccggcaggc attggacatt ttttgcgcga
    18241 tgctgggcac ggtcgcttcc aacctcgccc tgactttggg cgcgcgcggg ggcgtgtatt
    18301 tgtgcggcgg cattatcccg cgaatgttgg attacttcaa aacctccccc ttccgcagcc
    18361 gctttgaaaa caaagggcgt tttgaagcct accttgccgc gattcccgtt tatgtcgtct
    18421 tgagcgaatt tccgggtatt gccggcgcgg cggcggcgct cggcaaccac ctgaaaaacg
    18481 tctaaccacg gcagcccttg cagcagggcc gcattatcga agggcatatc attatgttaa
    18541 gcaaaatcag cgaatcactg gccgacctgt ccggtgcgga acgcaaagtt gccgaatgcg
    18601 ccttggcaga acccaagtgg tttgtccacg ccgccgtcgc cgaaatcgcc gaccgcgcct
    18661 cggtcagcca gccgaccgtc atccggttct gccgcagcct cggttataag gggctgccgg
    18721 aattcaagct ctccctgtcc gccagcatcg ggcacgaagg tatgccttat gtccacgaag
    18781 aactcaatgc cgacgacgat atggcgaatg tggttgaaaa agtgttgggc aacgccgccg
    18841 cagccttgtt gggggagagg aggttcttaa aagaatccga actcgaaaac ggcatcgcca
    18901 ccctgatgca cgcccgccgc gtcgaatttt acggcgtggg caattccggc attgtggcac
    18961 aggacgcgca gcacaaattt ttccgtttcg gcatgtccac cgtcgcctat gtcgatacgc
    19021 acacgcaact gatggcggca tccgttttga gcgatcagga tgttttggtt gccatttcca
    19081 acacgggttc gtctatcgaa cttttggatg cggtcagcat cgccaaagaa aacggcgcgt
    19141 ccgtcatcgc actgacccgc aacgattcgc ctctggcgca acttgccgac tgcgtgttga
    19201 gcgttgccac acaggaaaac gccgaactct acacgcccat ggtttcccgc ctcttgcagc
    19261 ttgccgtcat tgatattctc gccatcggac ttgccctgcg cttgggcgat gctgccagcc
    19321 tgcaattgca gaaaagcaaa aaaagcatac acaacaagca catcgattac gacaaagact
    19381 gatgttcaga cggcatcccg ccaatgccgt ctgaacggcc ggacaggctc cggcaagcct
    19441 tttatcagaa tttccttaat ggcggaaacc ccgcccttta aggcggcagg gttcgggttt
    19501 gccgcaaccc cttccaaatc ggggcggtac gcagggttgc gccttatgtg cgcatcgatg
    19561 tgcggaaacg ccccccgtca cggaaaacct caatcgggag catacagatg aaacaccttc
    19621 acgacttacc ggcctggtca aaattgtgga accactttga cgacagcaaa acattgcata
    19681 tgcgcgaaat gttcgagcaa gacccgcagc gggcggaacg ctactggctg caagtcggcg
    19741 gactgacgct ggactactcc aaaaaccgca tcaacgacga aaccatgtcc ctactgttcg
    19801 agttggcgcg tgaagcaggc gtgccggagc ggatgcggca gatgttccac ggcgaaaaaa
    19861 tcaataccac cgaaaaccgc gccgtcctgc atgtcgccct tcgcaaccgc accaattcgc
    19921 cgattatggt tgacggtgaa gatgtgatgc ccaaagtcaa ccgcgttttg caacgtatgg
    19981 gcgaatttgc acacgaagtc cgcagcggaa gctggctggg ctataccaac caagtcatta
    20041 ccgacgttgt caacatcggc atcggcggat cggatttggg cccgctgacg atgtgtaccg
    20101 cgctcaaacc tttcggtcat ccgcgcctca atatgcactt cgtctccaac gtggacggct
    20161 cgcaactgcg cgacgtattg tccaaagtcc acccggaaac cacgctgttc atcatcgcct
    20221 ccaaaacatt taccacgcag gaaacgctga ccaacgcgct aaccgcgcgc gaatggtttt
    20281 tgaatcatgc gggtgacgaa gaagccgttg ccaaacactt cgccgccgtt tccaccaatc
    20341 gaaaagccgt cgccgaattc ggcatcgaca tcgccaatat gtttgaattt tgggattggg
    20401 tcggcggtcg gtacagcctg tggtccgcca tcggattgcc gattatgctg tatctcggcg
    20461 aagaaaactt catcgaaatg ctcaacggcg cgcacctgat ggaccgacac ttcatcaaca
    20521 ccccgctcga gcgcaacctg cccgtcatcc tcgccctcat cggcatctgg tatatcaact
    20581 actacggtgg cggcagccac gtcatcgcgc cttacgacca acatttgcac cgcctgccca
    20641 aattcatcca gcagctcgat atggaaagca acggcaaaca ggttacgttg gacggcaaag
    20701 cagtcggaca cgaaacctcg ccgattatct ggggagaaac gggcattaac ggtcagcacg
    20761 ccttcttcca actgctgcat caaggcacgc acatcacccc catcgacctg attgcctcgc
    20821 ttgaaaaacg cagcaacctg cccggacacc acgaaatcct gcttgccaac gtctttgcac
    20881 aagcagaagc ctttatgtgc ggcaaaaccc ccgacgaagt ccgcgccgaa ctcaaagcgc
    20941 agggtatgga tgaggcgcgc atcgaagagc tggttccgca caaaaccttc tccggcaacc
    21001 gcccgaccaa cctcatcctg atggataagg tcaacccccg caatatgggc agcctgattg
    21061 ccatgtacga acacaaaacc ttcgtccaag gcatcatttg gggcatcaac agcttcgacc
    21121 agtggggcgt ggaactcggc aaacaactgg ctaaaaccat tttgggcgaa ctgacaggcg
    21181 aaaccgaacc tcaaaaacac gacagctcga ccgaacgcct gattaacctc tacctgcaga
    21241 ccaaccgcaa ataaaacctg cggaaaaatg ccgtctgaac gccgaccgtt tcagacggca
    21301 tttttatcga acaggaaaac cgtcggtaaa tttgcagggc gtgtgcaatc ccgatatggc
    21361 ggtttgcata aatttaaaca tatatgttcc gcagccatgt acttcaagtg ccgtagcgtg
    21421 tgtggaaata cgcaacagac agtccgtgtt tggacggcaa aggaaatcta atgaaaaaaa
    21481 atttacccgc actggcattg gcaagtatgc tgattttgtc gggctgcgac cgtttgggaa
    21541 taggcaaccc gttttccgga aaggaaattt cctgcggaag cgaagagact aaagagattt
    21601 tggtcaaact ggtccgcgac aatgtcgaag gtgaaaccgt caaaactttt gacgacgacg
    21661 cattcaaaga ccaagcattt gccgatatcg gcatatcgca tatccgcaga atggtcgaac
    21721 gtttgggcat aaccgtcgat gaagtccgaa ctaccgagaa aaccgacacg tccagcaaac
    21781 tcaaatgtga agccgcgtta aaactggacg tgcccgacga tgttgtcgat tatgccgtcg
    21841 ccgccaacca atctataggc aacagccata agaaaacgcc cgactttttt gaaccctact
    21901 accgcaaaga aggcgcgtat tatgtcaaaa ctatttctta cagcgtccag ccgacagacg
    21961 acaaaagcaa aatctttgcc gaactcagtc aggcacacga tatcatccat ccgctcagcg
    22021 agctggtgtc tatggcactg attaaagagc cgttggacaa agcgaaacaa aggaacgaaa
    22081 aacttgaagc ggcagaagcc accgcgcagg aagcgaggga ggcagaagaa gcggcggcgc
    22141 aggaggcatt gggtcgggag caggaagccg cccgcgtatc cgaatgggaa gaacgctaca
    22201 agctgtcgcg cagcgagttc gagcagtttt ggaaaggatt gcctcaaact gtacagaata
    22261 agctgcaagc ctcgcagaaa acatggaaaa gcggtatgga caagatctgt gccaacaatg
    22321 cgaaagccga aggtgaaacg ccaaacggca taaaagtcag tgagttggcg tgtaaaacgg
    22381 cagaaaccga agcacgcttg gaagagctgc acaaccgtaa aaaagccctt atcgacgaaa
    22441 tggtcaggga agaggacaag aaagaactgc caaagcggct ctgaacagcg cggttcaggc
    22501 actgcccgcc gatattgccg aaaccgttat gcccgagtac cgcaactggc aaaacggttt
    22561 gggtgccaaa tgcgccgacg gcgacgaata cggggtggca caatctgact gccgtaccag
    22621 agaaatcaat gcgaaaacca aagaaatcca aggttatctg attgactgaa acttggatgc
    22681 gggaatgtcg gcggcttttg cgtttttgtc gtttttaaat ccccttcgtt gcgtttggac
    22741 tttgccgata ccgcatatcc gagaaaggaa gcaatgattt actatcccgc cgtgctgttc
    22801 caagaccccg atcaaagcaa ctggggcgtg attattcccg atctgccggg ctgctatcct
    22861 gtcggcgata cggttgccga cgcgcttgcc gatgccaaag ccgccgcaat gttccatctc
    22921 gaagggcttt tgcaggaaaa cctgccgatt cccgaggcgc aaagcataga ggttaatcgg
    22981 gacaatcccg attatgccga tgcggtgttg tggacgatgg ttgaagcgga cgatgccgca
    23041 ttaaccggac aggtgcggtt taacgtcagt tggccgcaac atatcctcaa ccgtgtcgat
    23101 gcctgcactg ccgcccgtca tgaaacccga gcggtttttt ggcgaaagcc gccttaaagg
    23161 cgatgcagcc ttaactttca gcccgatttt ttcgggcttt tttattgccg atgccgtctg
    23221 aatgcggcgc ggcggcaatt ttaccgcgta tccctttcat tggcggcgcg gctttggttg
    23281 aaccgcgcgt cgtgctgcct gcctttcagt tcgctgatat acgcctgccg gcagtggtgg
    23341 cggtcgaaaa atacgccgtt gatcagggta tatatagtgg attaacaaaa accggt
//
LOCUS       DAXDXW010000026        23065 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXDXW010000026 DAXDXW010000000
VERSION     DAXDXW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.026"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..1146
                     /locus_tag="ACFB64_000906"
     CDS             <1..1146
                     /locus_tag="ACFB64_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFC1209285.1"
                     /translation="RGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDAL
                     EDEDVSESVDTSDKQPQDNTELHEKVEAVSLQPRAAQPRTQAAAQADAVSTNTNSALS
                     DAMASTQSILLDTGASLTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRRFSSKRT
                     QTQIGIDRSLSENMQIGGVLTYSDSQHTFDQAGGKNTFVQANLYGKYYLNDAWYVAGD
                     IGAGSLRSRLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKL
                     GDDSVKVSSMAVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGKSFAY
                     KADNQQQYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(1222..2424)
                     /gene="trpB"
                     /locus_tag="ACFB64_000907"
     CDS             complement(1222..2424)
                     /gene="trpB"
                     /locus_tag="ACFB64_000907"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC1209286.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALKELEQAYNEAKNDPE
                     FWAEFRRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     ARRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VIGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFYPYIEEENVRLVGVEAGGLGVDTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIEL"
     gene            complement(2507..3136)
                     /locus_tag="ACFB64_000908"
     CDS             complement(2507..3136)
                     /locus_tag="ACFB64_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC1209287.1"
                     /translation="MSENLLEIETHPFDPVLPPKAAVMMMGTFPPKEDKRAMQFHYPN
                     FQNDMWRVYGLVFFNDAAHFRMPSHGEAAHSEKAFDAEKIKAFLHERGIASCPTVLKA
                     AREHGNASDKFLKVVETVDLAAVLAKIPDCRHICTTGGKATEILLDIQGGGIKMPKTG
                     ETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAFFEMAGLCEKQL"
     gene            3412..4191
                     /gene="rsmA"
                     /locus_tag="ACFB64_000909"
     CDS             3412..4191
                     /gene="rsmA"
                     /locus_tag="ACFB64_000909"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC1209288.1"
                     /translation="MKEHKARKRFGQNFLQDTRIIGDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGISGKKKIVG
                     NLPYNISTPLLFKLAEVADDVADMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     ELLIDVPPESFDPAPKIDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFRQRRKTIRNN
                     LKELADDDDLQAVGISPQDRAEHIAPEKYVALSNYLADKAV"
     gene            4206..4775
                     /locus_tag="ACFB64_000910"
     CDS             4206..4775
                     /locus_tag="ACFB64_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HFC1209289.1"
                     /translation="MESNLQKTERLIREINRLHAQYSQDYFETGKVRKINLSHTLKNV
                     PTEHILSYRLNLHEAVNDYLAFADTRGIDFFYRVKTAESIHDKINRYLARGTQYPTNN
                     ILNDIFGARLIWPSETVAGILEKLDGWKTEYGLKNWYLRDAGGYIGIHIYFKNSSNFY
                     YPWELQVWDEKDAKANIENHMAYKRNFVR"
     gene            4886..5341
                     /locus_tag="ACFB64_000911"
     CDS             4886..5341
                     /locus_tag="ACFB64_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209290.1"
                     /translation="MKYTAALLTFLLTACMNPNDAFFQNRRYQMPEAQLNGSNAVFHY
                     GYSQNPDHDLLVDQVRVPDAYRASFARDWIAKEQGYRKNDCVLEKAESGGVTYYTCDA
                     EKGFNGTRFVIYVVNMKNDKGYVKVYRGMNKPNQEELTKLVADLNKFYP"
     gene            5383..6111
                     /locus_tag="ACFB64_000912"
     CDS             5383..6111
                     /locus_tag="ACFB64_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC1209291.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLDNITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            6184..6543
                     /locus_tag="ACFB64_000913"
     CDS             6184..6543
                     /locus_tag="ACFB64_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209292.1"
                     /translation="MYFVDRTAAVLKPTARFLEWLKSTDENMPDLTIEQLRADCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            6548..6988
                     /locus_tag="ACFB64_000914"
     CDS             6548..6988
                     /locus_tag="ACFB64_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC1209293.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASIAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGRAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGKTVRQCIMPDMTDKESYRRLKVWVLWRQPKKTAETDTSD"
     gene            7019..8293
                     /gene="folC"
                     /locus_tag="ACFB64_000915"
     CDS             7019..8293
                     /gene="folC"
                     /locus_tag="ACFB64_000915"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC1209294.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVSEVKKRMNLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKTGTLTSPHLLHYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFIREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGAYQLSNAACALTVLECLDDRLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRNLINLAYAQKRTAV
                     FSMLSDKDIDGVSETVKDQFDEWYIAPLDVPRGMTADALKAKLEQHHIENIQTFAAVR
                     DAYRAAASKAGEDDRIVVFGSFHTVADVMSVL"
     gene            8307..9347
                     /gene="ftsN"
                     /locus_tag="ACFB64_000916"
     CDS             8307..9347
                     /gene="ftsN"
                     /locus_tag="ACFB64_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC1209295.1"
                     /translation="MSENKQNEVLTGYEQLKRRNRRRLVTASSLVAASCILLAAALSS
                     DPADSNPAPQAGETGATESQTANTAQTPALKSAAENGETAADKPQDLAGEDKPSAADS
                     EISEPENVGAPLVLINDRLEDSNIKGLEESEKLQQAETAKTEPKQAKQRAAEKVSATA
                     DSTDTVAVEKPKRSAEPKPQKAERTAEAKPKAKETKTAEKVADKPKTAAEKTKPDTAK
                     SDSAVKEAKKADKAEGKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKAGKKAA
                     IQAGYAEKERALSLQRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKL
                     RVHGIAGQVTNE"
     gene            9340..9837
                     /locus_tag="ACFB64_000917"
     CDS             9340..9837
                     /locus_tag="ACFB64_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC1209296.1"
                     /translation="MNSLPIADLLASAVIAACIVISTMRGVIAEAGSMVAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLIMLASKTDLPDTEEWQQSYTVPFFVSLSEAVLNHTDNAPE
                     SLDDD"
     gene            10031..11575
                     /gene="purF"
                     /locus_tag="ACFB64_000918"
     CDS             10031..11575
                     /gene="purF"
                     /locus_tag="ACFB64_000918"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC1209297.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVSEVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHINTGSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMVAFRDPYGIRPLALGSQTDSEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFNGTIIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLNKPYREGLIKNR
                     YIGRTFIMPGQSTRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYRTGDIDDAYLDRLSAEKSGCAGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            11675..12166
                     /gene="greB"
                     /locus_tag="ACFB64_000919"
     CDS             11675..12166
                     /gene="greB"
                     /locus_tag="ACFB64_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC1209298.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGVDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            12225..12851
                     /locus_tag="ACFB64_000920"
     CDS             12225..12851
                     /locus_tag="ACFB64_000920"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC1209299.1"
                     /translation="MRKIRTKICGITTPEDALYAAHAGADALGLVFYPQSPRAIDIIK
                     AQKIAAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAATRFPNAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
     gene            complement(13325..16156)
                     /gene="lbpA"
                     /locus_tag="ACFB64_000921"
                     /gene_synonym="tbpA"
     CDS             complement(13325..16156)
                     /gene="lbpA"
                     /locus_tag="ACFB64_000921"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFC1209300.1"
                     /translation="MNKKHGFPLTLTALAIATAFPAYAAQAGAAALDAAQSQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSRGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVEYGIDRLDAFRQTYDIKRKTTEPFFLVEGENTLK
                     PVAKLAGYGIYLNRQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKNLVKGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEKYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISESTG
                     FDEKNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRQNFTTSEELVRSGRYADRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADQ
                     KTKLPDSAGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSAGRVTSSWKTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(16153..18342)
                     /locus_tag="ACFB64_000922"
     CDS             complement(16153..18342)
                     /locus_tag="ACFB64_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC1209301.1"
                     /translation="MRKLNYYGIALLPLMLASCGGNFGVQPVVESTPTAYPVTFKSKD
                     VPTSPPAEPSVETTPVNRPAVGAAMRLLRRNTAFHREDGTAIPDSKQAEEKLSFKEGD
                     VLFLYGSKGNKLQQLKSEIHKRDSDVEIRTSEKENKKYNYKFVDAGYVYTKNGKDEIE
                     QNSGGKRFTHRFGYDGFVYYSGERPSQSLPSAGTVKYFGNWQYMTDAKRHRTGKAVAS
                     DDLGYITFYGNDIGATSYAAKDADDREKHPAEYTVDFDKKILKGELIKNQYVQKRNDP
                     KEPKKPLTIYNITADLNGNRFTGSAKVNTEVKTRHADKEYLFFHTDADQRLEGGFFGD
                     NGEELAGRFISNDNGVFGVFAGKQKTNASGTNPAMPFGKHTKILDSLKISVDEATDEN
                     PRPFEVSTMPDFGHPDKLLVEGREIPLVSKEKTIDLADGRKMTVSACCDFLTYVKLGR
                     IKTERPAVKPKAQDEEDSGINNGEESEDEEIAEESEDEVSEDDNGEDEDEIVEEEADE
                     AEEIEEEAEEEEPEEESPEEGNGVSDGIPPAPEALKGRDIDLFLKGIRTAEADIPKTG
                     TAHYTGTWEARIGEPIQWDNKADKAAKAEFDVDFGNKSISGTLTEQNGVEPAFRIENG
                     VIEGNGFHATARTRDNGINLSGNGSTNPQSFKADNLLVTGGFYGPQAAELGGTIFNKD
                     GKSLGITEDIENEVENEADVGEQLEPEVKPQFGVVFGAKKDNKEVEK"
     gene            complement(18495..19418)
                     /gene="era"
                     /locus_tag="ACFB64_000923"
     CDS             complement(18495..19418)
                     /gene="era"
                     /locus_tag="ACFB64_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC1209302.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRLTDADRVVLKQLPKHTPVILVINKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEGDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDNKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(19465..20184)
                     /gene="rnc"
                     /locus_tag="ACFB64_000924"
     CDS             complement(19465..20184)
                     /gene="rnc"
                     /locus_tag="ACFB64_000924"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC1209303.1"
                     /translation="MKDDVLKRQAHTAIQKKLGYAFRDMSLLRRALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFAERVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            20352..20675
                     /locus_tag="ACFB64_000925"
     CDS             20352..20675
                     /locus_tag="ACFB64_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC1209304.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            20722..21198
                     /gene="ribH"
                     /locus_tag="ACFB64_000926"
     CDS             20722..21198
                     /gene="ribH"
                     /locus_tag="ACFB64_000926"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC1209305.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSQMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVANESGAGIGR
                     VALDYNIPIANAVLTTENDAQAIERIGEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            21276..21701
                     /gene="nusB"
                     /locus_tag="ACFB64_000927"
     CDS             21276..21701
                     /gene="nusB"
                     /locus_tag="ACFB64_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC1209306.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(21780..22814)
                     /gene="pyrC"
                     /locus_tag="ACFB64_000928"
     CDS             complement(21780..22814)
                     /gene="pyrC"
                     /locus_tag="ACFB64_000928"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC1209307.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASENGARFYGIPENADTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(22875..>23065)
                     /locus_tag="ACFB64_000929"
     CDS             complement(22875..>23065)
                     /locus_tag="ACFB64_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209308.1"
                     /translation="LRAEQSVQAPFETRQISYAWRESGSPEETVLTAQTADSPDLPVQ
                     AVLNLTHRSHNGKPLILP"
BASE COUNT         5560 a         6544 c         5734 g         5227 t
ORIGIN      
        1 cgcggacgca gatctactct gccggcaccg ccctcgccat catttgattc atcagcttac
       61 gcagcaccca gggccttgca taatccggac tggtatgaaa atgattatga agaaatcccc
      121 ttggatgcgt tggaagatga agatgtatcc gaatcggttg acacatcaga caaacagcct
      181 caagacaata cggaacttca tgaaaaagtt gaggcagtga gtttgcaacc aagagccgcg
      241 cagccgcgaa cccaagccgc cgcgcaagcc gatgcagtca gcaccaatac taactcggct
      301 ttatctgacg caatggcaag cacgcaatct atcttgttgg atacaggtgc ttcattaaca
      361 cggcacattg cacaaaaatc acgcgctgat gccgaaaaaa acagtgtttg gatgtcaaac
      421 accggttatg gccgtgatta tgcttccgca caatatcgcc ggtttagttc gaaacgcacg
      481 caaacacaaa tcggcattga ccgcagcttg tccgaaaata tgcagatagg cggagtattg
      541 acttactctg acagtcagca tacttttgat caggcgggcg gcaaaaatac ttttgtgcaa
      601 gccaaccttt atggtaagta ttatttaaat gatgcttggt atgtggccgg cgatattggt
      661 gcgggcagct tgagaagccg gttacaaacg cagcaaaaag caaactttaa ccgaacaagc
      721 atccaaaccg gccttacttt gggcaatacg ctgaaaatca atcaattcga gattgtccct
      781 agtgcgggta tccgttacag ccgcctgtca tctgcagatt acaagttggg tgacgacagt
      841 gttaaagtaa gttctatggc agtgaaaaca ctaacggccg gactggattt tgcttatcgg
      901 tttaaagtcg gcaaccttac cgtaaaaccc ttgttatctg cagcttactt tgccaattat
      961 ggcaaaggcg gcgtgaatgt gggcggtaaa tccttcgcct ataaagcaga taatcaacag
     1021 caatattcag caggcgccgc gttactgtac cgtaatgtta cattaaacgt aaatggcagt
     1081 attacaaaag gcaaacaatt ggaaaaacaa aaatccggac aaattaaaat acagattcgt
     1141 ttctaaaata ccaaattcat agcaaaataa aatgccgtct gaactcaagc ttcggacggc
     1201 atttttatca gactaacaaa gctacagctc aatgcctttg agtttcgcca cggtattgat
     1261 gtctttgtcg ccgcgacccg acaggttgac caaaatcact tggtctttac ccattttcgg
     1321 cgcgtttttc accgcccaag caacggcgtg gctggattcg agcgcgggga tgatgccctc
     1381 gaatcggcag agcaagtcaa aggcttcgag tgcttcgtca tctttggcaa cggtgtattc
     1441 gacgcgcttg atgtcgtgca gatggctgtg ttccgggccg atgccggggt aatccaagcc
     1501 tgcggaaaca gagtgcgtcc ccaagacttg gccgttttcg tcctgcatca gatagctgcg
     1561 gaagccgtgc agtacgccaa tcggtgcgcc cgaagtaatc ggcgcggcgt ggtcaggcgt
     1621 atccacgccc aaaccgcctg cttctacgcc gaccaaacgt acgttttctt cttcgatata
     1681 gggatagaac aaaccgatgg cgttcgagcc gccgccgacg caggcaacgg cgacgtcggg
     1741 ctgtctgccg atagcttcct gcatctgcgc tttggcttcg ttgccgatga cgcattggaa
     1801 atcacgcacc atttcgggat acggcgcggg gccggcggcg gtgccgatga tgtagaacgt
     1861 atcgtccact cgggcgaccc attcgcgcat cgcttcgttc atcgcgtctt tcaaagtacg
     1921 gctgccactg tcaacgccga tgacattcgc accaagcagc ttcatacgga acacattcgg
     1981 catttggcgt tgaatatcgt ccgcacccat gtacacgtcg caagtcatgc cgaaacgtgc
     2041 ggcgacggtg gcagaagcca cgccgtgctg acccgcgccg gtttcggcga tgacgcgttt
     2101 tttgcccata cggcgggcaa gcaatgcctg accgatggtg ttgttgacct tgtgcgcgcc
     2161 ggtgtggttc aagtcttcgc gcttcaacca gatttgcgcg ccgcccaaat gctcggacaa
     2221 ccgcgcggcg tggtaaacgg ggctgggcct gccgacataa tgtttcaaat cgcggcggaa
     2281 ctccgcccaa aattcaggat cgtttttcgc ttcgttgtag gcctgttcca gctctttcaa
     2341 agcgggaatc agggtttcgg aaacataaag cccgccgtgt tcgccgaaaa agcccttttc
     2401 gtcgggcgcg tggtagttct tcatcggatt cttccttatg cttcaggttt tcagacggca
     2461 tttccgtttt ttcaaacatg ccgtctgaac gggaaattgt cggcaattat aactgctttt
     2521 cacacaagcc cgccatttcg aaaaacgccc gatacgccgc tgcctttttc gccaaactca
     2581 aaggataggc gcgcgaagtc gaaggcaggc gcgtcagtgt caaatcccgt ccggcaaacg
     2641 gaaacggcac ggtttcgccc gttttcggca ttttgatgcc gccgccctga atatcgagca
     2701 ggatttccgt cgccttgccg cctgtcgtac aaatatggcg gcagtctggt attttcgcca
     2761 acaccgccgc caaatcgacg gtttcaacta cctttaaaaa cttgtcggac gcattgccgt
     2821 gttcccttgc cgccttcaac acggtcggac aggacgcaat cccccgttcg tgcaaaaacg
     2881 ccttgatttt ctccgcgtca aacgcttttt cagagtgcgc agcctccccg tgggacggca
     2941 tccggaaatg cgccgcatcg ttaaaaaaca ccagcccgta aacgcgccac atatcgtttt
     3001 ggaaattcgg ataatgaaac tgcatcgcgc gtttgtcttc cttgggcgga aacgtcccca
     3061 tcatcatgac cgcagccttc ggcggcaaca cgggatcgaa gggatgggtt tcgatttcaa
     3121 gcagattttc agacacgttc aatcctccaa caggcaaagc ggcagacagc cggtttgcac
     3181 caaaccgcca aacacggcaa gccttcaaac agcattatca cgccctgccg ttttcggtta
     3241 caatgccgac atcaaacgga tactgtaaac acatttatgt tccaacacac aggacgacac
     3301 ataaagcacc gccctatgtg tcgtcctgat ttggaagggg ttacgccccg cccaaacaga
     3361 gtctgatcct gccgccctaa agggcggggt ttcaaccgaa gaggaaacac gatgaaagaa
     3421 cacaaagccc gcaagcgttt cgggcagaat tttttgcagg acacgcggat tatcggcgat
     3481 attgtcaacg ccgtgcgccc gcaggcggat gatgtcgtga ttgaaatcgg cccgggtttg
     3541 gcggcaatta ccgaaccttt ggcgaaaaag ctgaaccgcc tgcacgttgt cgaaatcgac
     3601 cgcgacatcg tatgccgtct gaaaacgctg ccgtttgcgg ataaactggt gattcacgaa
     3661 ggcgacgtgt tgcagtttga tttcaacggc atctcaggca aaaagaaaat cgtcggcaac
     3721 ctgccgtata acatctccac gccgcttttg ttcaaactgg cggaggtggc ggacgatgtc
     3781 gccgatatgc actttatgtt gcaaaaagaa gtggtcgagc gcatggttgc cgcgccgaaa
     3841 agcaacgact acggtcgctt gggtgtgatg ctgcaatatt ttttcgatat ggagctgctg
     3901 attgacgtgc cgcccgaatc gttcgatccc gcgccgaaaa tcgattccgc agtggtgcgc
     3961 atgattccgg tgaaacaccg tatcggcaag gcggacgatt tcgagcattt cgccaaactc
     4021 gtgaaactcg ccttccgcca acgccgcaaa accatacgca acaatctgaa agagcttgcc
     4081 gacgacgatg atttgcaggc agtcggcatc agtccgcaag accgtgccga acacatcgcg
     4141 ccggagaaat atgtggcgtt gagcaattat ctggcggaca aggccgtctg aaaaggaacg
     4201 cggcgatgga aagcaacctg cagaaaaccg agcgtttgat ccgcgaaatc aaccgcctgc
     4261 acgcacaata ttcgcaggat tattttgaaa ccggcaaagt gcggaaaatc aatttgagcc
     4321 acacgctcaa aaacgttccg accgaacata ttttgtctta ccgccttaat ttgcacgaag
     4381 cagttaacga ttatttagcc tttgccgata cgcgcggcat agactttttc taccgcgtga
     4441 aaaccgcaga aagcatacat gataaaatca accgctatct ggcacgcggc acccaatatc
     4501 cgaccaacaa catcctgaac gatattttcg gcgcgcggct gatttggccg tctgaaacag
     4561 tcgccgggat tttagaaaaa cttgacggct ggaaaacaga atacggcttg aaaaactggt
     4621 atttgcgcga tgccggcggc tatatcggca tacacattta tttcaaaaac agcagcaatt
     4681 tctactatcc gtgggaattg caggtttggg atgaaaaaga tgccaaagcc aatattgaaa
     4741 accatatggc gtacaaacgg aatttcgtgc gttgaattgc cggcaagccg ccttgaaacc
     4801 gtttataact gtttataaga gaataaacca taaaatacac aaacgtattc ctgacttttc
     4861 ctttaaccgc ccgagtgaac aaactatgaa atacacagcc gcactcctga cttttctttt
     4921 aactgcctgc atgaacccga acgatgcgtt tttccaaaac cggcgttatc aaatgccgga
     4981 ggcgcaattg aacggttcaa acgccgtttt ccattacggt tattcccaaa atcccgatca
     5041 tgatttattg gtcgaccaag tccgcgtgcc ggacgcgtac agggcttcat ttgcgcggga
     5101 ttggatagcg aaggagcaag gttaccgcaa aaacgactgc gtactggaaa aagcggaaag
     5161 cgggggtgtg acgtactaca cctgcgatgc ggaaaaagga ttcaacggca cacgtttcgt
     5221 catctatgtc gtgaatatga aaaacgacaa aggctacgtc aaagtttacc ggggcatgaa
     5281 taagccgaat caggaagagt taaccaaatt ggttgccgat ttgaataagt tttaccctta
     5341 atcagccaga cggattcaga ctgcccgaaa agaatggaca ctatgatcaa attcaaaaac
     5401 gtacacaaac atttcaaaga cctgcacgtc atcaacggcg taaacttgga aatcaaaaaa
     5461 ggcgaagtgg tcgtcgtctg cgggccttcg ggcagcggca aatccacact catccgcacc
     5521 gtcaaccagc ttgaaagcat cgaaagcggc gagatttggg tggacggcgt caacgttgcc
     5581 gaccccaaaa ccgatttgaa caaaatccgc gaagaagtcg gctttgtgtt tcaaagtttc
     5641 aacctctacc cgcacctgac cgtgttggac aacatcaccc tcgcaccgat gaaggttaag
     5701 gggcaaaatg cggaacaggc ggagaaaaag gcaatggagc ttttggaacg cgtcggactg
     5761 gcacacaaaa aagacgcctt cccttcccaa ctttccggcg gccagcagca acgcgtggcg
     5821 attgcgcgcg ggctggcgat ggagccgcgc gtgatgctgt ttgacgagcc gacttccgca
     5881 ctcgaccccg aaatggtcgg cgaagtgttg aaagtgatga aagacttggc ggaaagcggc
     5941 atgacgatga tgtgcgtaac ccacgaaatg gggtttgccc gcgaagttgc cgaccgcgtg
     6001 attttcgtcg ataaagggca aatccttgaa gacgaaacgc cggaagcgtt tttcaccaac
     6061 ccgaaacacg aacgcgccaa gcagttcctg caacaagtca tgacccattg atgctatgcc
     6121 gtctgaaaac cgaacgttca gacggcatag cccgacaccg caagatgatt gagaaagcac
     6181 accatgtatt ttgttgaccg caccgccgcc gtcctcaagc cgaccgcgcg ctttttggaa
     6241 tggctcaaaa gcaccgacga aaatatgccc gacctgacca tagaacagct tcgcgccgac
     6301 tgttccgtct tcctcgttcc gcagttcgac gaaccggaag ccgtcgtttc ctacttcgac
     6361 gaacgctacc ggcagatttt tgaggcggag ctggcaggtt gggacatcga caaggacaaa
     6421 tggccgcaag atatggggct gaaggcgttt tgggaatttt tcgacattga aatccacgat
     6481 atggttttgg atatggaaga agcggatttg aacatcacgc ccgtgttcga caatatgatg
     6541 taacgccatg cgcgcctttc agacggcatt gaggccgtcc cgcatcctga acatcctgac
     6601 cgtatcactc cactcggctt ccattgccgt ctgcctgact tggttttacg gccggatgat
     6661 gtggttcggt ttggcggctt tggcggcaag ttacgcatac tcgctcagga taacgaatct
     6721 gaaacaccgc catgccataa ccgccattac catagaccgc gacggacggg cggaaatcgt
     6781 atccggcaaa gacaaaacag cggcaacact tgcgggcagc agtatggtta cgccttatgc
     6841 cttattctta caatgggaca caggcggcaa aaccgtccgc caatgcatca tgcccgacat
     6901 gacggacaaa gaatcctacc ggcggctgaa agtctgggtg ctttggcggc agccgaaaaa
     6961 aaccgccgaa accgatacat cagactgatt tcagacggca tttgaaaacg aaacactcat
     7021 gaaaacacta caagactggc tctcgcattt ggaaaccgcc cacagcggcg gtttgatcga
     7081 catgggtttg gagcgcgtaa gcgaagtaaa aaagcgcatg aacctgacac cgcaatgccc
     7141 cgtcgtcgtc gtcgcgggaa caaacggcaa aggttcggtc tgcgcctacc tgacacagat
     7201 ttacaaacag gcaggcttca aaaccggcac gctgaccagc ccgcatttat tgcattacaa
     7261 cgaacgcatc gccatcaacg ccgaacccgt ttcggacgat acgattattg cctctttcga
     7321 gcgcatcgaa gccgcgcgcg gcgaaatatc gctgacttat tttgaattca atgccttggc
     7381 ggctgtcgac attttcatac gcgaacaagt ggatgtaatg atattggaag tcggcttggg
     7441 cggacgtttg gacgcggtca acgcgtttga cggcgattgc gcggtggtta ctagtgtgga
     7501 tttggaccat caggcatttt tgggcgatac ggtcgaacag gtcggctttg aaaaggcagg
     7561 cgtgttccgc agcggcaaac ccgccatctg cggtcaaaac cccgcgcccg catcattggt
     7621 cgcacacgcc gaagccatag gcgcgaaact gctgatggta cagcgcgatt tcgagtttca
     7681 cgcgatggaa aacatccaat ggaactaccg cttccggccg cagcattcag acggtcccgc
     7741 acgcaaccgc aatgccctgc ccttccccgc attgcgcggc gcataccagc tttctaacgc
     7801 cgcctgcgcg ttgaccgtat tggaatgcct cgacgacaga ctgccggtgg acattggtgc
     7861 aatcaaacgc gggctgctgc tggttgaaaa tcccggacgc ttccaagtcc tgcccggccg
     7921 gccgctgacc gtattggatg tcggacacaa cccccacgcc gcccgcgccc tgcgccgcaa
     7981 cctaatcaac cttgcctacg cgcaaaagcg caccgccgtg ttcagtatgc tgtccgacaa
     8041 agacatagac ggcgtgtcgg aaaccgttaa agaccagttt gacgagtggt atatcgcgcc
     8101 tttggacgtg ccgcgcggta tgacggcgga tgcactgaaa gccaaattgg aacaacacca
     8161 catcgaaaac atacagacat tcgccgccgt ccgcgacgcg taccgggcgg cagcctcaaa
     8221 agcgggcgaa gacgacagaa tcgtcgtatt cggctcattt cataccgtcg ccgacgtaat
     8281 gtcggtactg tgaaaaggaa acttctatgt ccgaaaacaa acaaaacgaa gtcctgaccg
     8341 gttacgaaca gctgaaacgg cgcaaccgcc gccgcctcgt aacggcaagc tccctggttg
     8401 ccgcctcctg catcctgctg gcagccgcac tcagttccga tcctgccgac agcaatcccg
     8461 caccgcaggc cggcgaaacc ggcgcaacgg aaagccaaac ggcaaacacg gcacaaaccc
     8521 ctgccttgaa atccgccgcc gaaaacgggg aaaccgccgc cgacaaaccg caggacttgg
     8581 caggcgaaga caagccttct gccgccgaca gcgaaatcag cgagcctgaa aacgtaggcg
     8641 cgccgctggt gctgattaac gaccggctcg aagacagcaa catcaaaggt ttggaagaat
     8701 ccgagaaact gcaacaggca gaaaccgcca aaaccgaacc gaagcaggca aaacaacgcg
     8761 ctgccgaaaa agtgtcggca actgccgaca gtacggatac ggtagcggtt gaaaaaccga
     8821 aacgctctgc cgaacccaaa ccgcaaaaag cggaacgcac tgccgaagcc aagcccaaag
     8881 ccaaagaaac caaaaccgcc gaaaaagttg ccgacaaacc gaaaactgct gccgaaaaaa
     8941 ccaaaccgga tacggcaaaa tccgacagcg cggtaaaaga agcgaaaaaa gccgacaagg
     9001 ctgaaggcaa aaagacagcc gaaaaagacc gttcggacgg caaaaaacac gaaacggcgc
     9061 aaaaaaccga caaagcggac aaaaccaaaa ccgccgagaa ggaaaaatcc ggcaaggcgg
     9121 gcaaaaaagc cgccattcag gcaggttatg ccgaaaaaga acgcgccttg agcctccagc
     9181 gcaaaatgaa ggcggcgggt atcgattcga ccatcaccga aatcatgacc gacaacggca
     9241 aagtttaccg cgtcaaatca agcaactata aaaacgcaag ggatgccgaa cgcgatttga
     9301 acaaactgcg cgtgcacggc atcgccggcc aggtaacgaa tgaatagcct ccccattgcc
     9361 gacctcctcg cctccgccgt catcgccgcc tgcatcgtca tttccacgat gcgcggcgtg
     9421 attgcggaag caggttcgat ggtggcatgg gtggtttcct tcttttttgc caaactcttt
     9481 gccgcaccct tcgccgacct cgcctttgcc tcgttccaac cccgcctgtt tgcattggct
     9541 ctgtcattca tttccctgtt cgtcattgcc tgtctgatcc agaaaatgct ccgttcgctg
     9601 ctgaccggcg cagtttcggc ggtcggtctg ggctttgcca accgcatttt gggcggtgta
     9661 ttcggtgcat tgaaaggcgt tttgattgtt accctgctga tcatgcttgc ttcaaaaacc
     9721 gacctgcccg ataccgaaga atggcaacag tcctataccg taccgttttt cgtatcgctt
     9781 tccgaagcgg tgttaaacca tacggacaac gcacccgaat ccctcgacga cgactaatcc
     9841 gcatacgtgg cgtttcagac agcatacctg atttatgccg tccgaaacgc cttttctttc
     9901 cttgacacac cccgacagac aaagcgcacc tgcctgactt tacacaaata ttgtcaacaa
     9961 ttcgggcaaa aagccgccgt ttgctttata attccgtttc acccttaaac ctcgaatcgg
    10021 agaagacgat atgtgcggcg tattaggttt ggtcagtcat gaacccgtga accagcttct
    10081 gtacgacggt ttgcagatgt tgcagcacag ggggcaggat gcggcgggca ttgcaacggc
    10141 ggaaggcggt accttccata tgcacaaagg caaagggatg gtgagcgaag tgttccgcac
    10201 gcgcaatatg cgcgatttga ccggcaacgc cggcatcgcc cacgtccgtt atcccacggc
    10261 gggcaacgcc ggcagcagcg cggaggcgca gcctttctac gtcagctcgc ctttcggcat
    10321 cgttttggcg cacaacggca acctcaccaa cactgccgaa ctgtatgaaa acgtgtgtaa
    10381 caaacacctg cgccacatca acaccggctc cgattccgaa gttttgctca acgtattcgc
    10441 gcacgaattg cgccgcgaag tctctaaaaa cgccgacccg caccggctca atgccgacaa
    10501 tattttcaac gccgttgccg aagtccaccg cctggtgcgc ggtgcatacg gcgtggttgc
    10561 catgattgcg ggctacggca tggttgcctt ccgcgatcct tacggcatcc gcccgctggc
    10621 attgggttcg caaaccgaca gcgaaggcag gaaatcctat gccgtcgcct ccgaatccgt
    10681 cgccttcaac gcgcttgcct acgatttgga acgcgatatc cggccgggcg aagcggtatt
    10741 tgtcggcttt aacggcacaa tcatcgcccg acaatgcagc gaccgcgcca aactcagccc
    10801 ctgccttttt gaatatgttt attttgcccg ccccgactct gtgattgacg gcgtgtccgt
    10861 ttaccagtcg cgcttggata tgggcgtgtc cttggcggaa aaaatcaaac gcgagctgcc
    10921 cgtggacggc atcgacgtcg tgatgcccat tcccgatacc agccgcccca gtgcaatgga
    10981 gcttgccgtc cacctcaaca aaccctaccg cgagggtttg attaaaaacc gctatatcgg
    11041 ccgcaccttt atcatgcccg gtcagtcgac gcgcaaaaaa tccgtgcgcc aaaaactcag
    11101 cccgatggaa accgaatttg caggcaaaag cgtgttgctg gtggacgact ccatcgtgcg
    11161 cgggacgacc agccgcgaaa tcgtcgaaat ggtacgcgcg gcgggcgcgc gcaaagtcta
    11221 tatcgcctcc gccgcgcccg aagtgcgcta tcccaatgtg tacggcatcg atatgcccac
    11281 gcgcgaagag ttgattgcca acgggcgcag cgcggcggaa atcgccgccg aaatcggcgc
    11341 ggacggcatc gtgtttcaag acttgggcga tttggaagcc gtcgtcaaag cactcaaccc
    11401 gaaaatcgaa tccttcgatt cgtcctgttt caacggcatt tatcggaccg gcgacatcga
    11461 tgatgcctac ctcgaccgcc tgtctgccga gaaatccggc tgcgccggtc tgaaaatcca
    11521 cccgagcagg atggaacaca gcatcagcat cagcgatgcg ggcgacgaag aataaggctt
    11581 cccaaaccgc gatgccgtct gaaatccgaa cgttgggttt tcagacggca tttcccatat
    11641 cggcatcaca tttcccgttt catccgcaca caatatgagt accgaaacca aaaactacat
    11701 tacgcccgcc ggctggcagg cattgaaaga cgagctttat caactggtca ataaagaacg
    11761 ccccgaaatc gtccaaatcg tcaactgggc ggcaggcaac ggcgaccgca gcgaaaacgg
    11821 cgactatctt tacggcaaac gccgtatgcg cgaaatcgac cgccgcatcc gtttcctgac
    11881 caaacgtttg gaggctgccg tcgtcgtcga tcccgaattg cgcgaagcga ccgaccaagt
    11941 atttttcggc gcgaccgtcg gtctgttgcg cggcgacggg cgcgagcaaa ccgtcaaaat
    12001 cgtcggcgtc gatgaaatcg ataccgcgca aaacaaaatt tcttggattt cgccgttggc
    12061 gcgttgcctg attaaagcgc gcgaaggcga cgaagtggtt ttgaacacgc cggaaggccg
    12121 tgaggaaatt gaaatccttt ctgtcgaata catcaaaatc gactgatcgg gaatgccgtc
    12181 tgaaacggca caagcgcacc gtttcccaaa taaaggaata ccgaatgagg aaaatccgca
    12241 ccaaaatctg cggcatcacc acaccggaag acgcactgta tgccgcccac gccggcgcag
    12301 acgcattggg actggttttt tacccccaaa gcccccgcgc tatcgacatc attaaagcac
    12361 aaaaaatcgc cgccgcactg ccgccgtttg tcagcgttgt cgcccttttc gtcaacgaaa
    12421 gcgcgcaaaa catccgccgc atccttgccg aagtgccgat acacatcatc caattccacg
    12481 gcgacgaaga cgatgcattc tgccggcagt tcgaccgccc ctatattaaa gccattcgtg
    12541 ttcagacggc atcagacatc cgaaacgccg ccacgcgctt ccccaacgct caggcactgc
    12601 tgttcgatgc ctatcaccct tcggaatacg gcggcaccgg acaccgcttc gactggacgc
    12661 tgttggcgga atattcgggc aagccgtggg tgcttgccgg cgggctgacc cctgaaaacg
    12721 tcggcgaagc cgtccgcatc accggagcgg aagcggtcga cgtatccggc ggcgtggaag
    12781 cgtctaaagg caaaaaagac cccgccaaag tcgccgcctt tatcgcaacc gccaaccgcc
    12841 tatcccgtta aagcaacaaa aattgccgcc ggaatgactt atagtggatt aacaaaaacc
    12901 agtacggcgt tgcctcgcct tgccgtacta tttgtactgt ctgcggctcg ccgccttgtc
    12961 ctgatttttg ttaatccact ataatctcaa aaatttatgc cattaaatca ataatttctg
    13021 atgaattttg aaaacttaat cccgtcattc ccgcgcaggc gggaacccgg ttcattgagc
    13081 ttcagctatt tagaataaat tttgaaactc taatcgcgtc attcccacga aagtgggaat
    13141 ccggtttttt gagtttcagt catttccgat aaattgcctt agcattgaat gtccggattc
    13201 ccgcctgcgc gggaatgacg gcggaaatct tgtttatatt gaatcaaaaa aacctgcacc
    13261 ttaatcagtt ggcggtttag tccgactttt ggggtgcaga tcaagcttcc agacggcatt
    13321 tcctttaaaa cttcatttcg agcgcgaggc tgaagttcct gcccggtgcg gcataccttc
    13381 catagttgct gtcgccgccg tgccggtttg ccgtgctttc cgcagtctgg cgcaaggatt
    13441 cccaagtaac gtagcggtag ttgccgatat tgtagatagc cgccctcaag gtcagccgtt
    13501 ttttcagatt cagataagcg gaaacatctg ccgttttcca agaagacgta acccttcctg
    13561 ccgaatatcg tttttgatcg cctgccagat aagcaagctc gtcagggttt ttccctttgg
    13621 aataggtcag cataatgttt gcgccccatt tcccctcggg ctggtcgtat ccgaacccca
    13681 aaacataacg cgacggctgt accgcatcca aagcatagct gcggagggac aagtccggcc
    13741 ggttggatac cgatttcggt ttgatacggt tgtacgccaa tgtggtgtac aggccttcgg
    13801 gcagtttgcc gtatacgccg ttccagtcga ttttccccaa gatgttgatg ccttgaagcg
    13861 acatattttg ggcattgtaa taatcgcgta tatcaatctc tgtcaatcgt cctgctgaat
    13921 ccggcaattt ggttttttga tcggcaacgg caatcatatc ggtataacgg ttgcggaaac
    13981 tgctgatttc caaaaagccg aaatcgccct tccactgcaa accgatttcc cggttggctg
    14041 ccttttccga tttcagggcg ggacgctgcc agcctttcgg ataatcgtga taaatgtcta
    14101 tcccgaaaag ttcttggaag gaaggcgttc tgaagccgct ggaggcgcgg taagacacgg
    14161 aaaaatgccg gttcggtttg aacacgatgc cgctgttcca cgaacggtcg gcataccgcc
    14221 cgctgcggac gagttcttcc gacgtggtga agttttgccg gtcatacctg ccgcccaagc
    14281 tgaaatcaaa atatttgccg attgaaaaac ggtcgttcaa agaaatgtgg atattgctgc
    14341 cgttgatttt tcttggcacg catttgcggg aacgcagggt ttcgatgtag ccgcagaccg
    14401 acccttcgac gacttcgggc ttacccaaac ggtacttatc ttgattcttt tcatcgaatc
    14461 ccgtggattc cgaaatcctt gccgcattgt gggaaagctg ttcggggcgg gagatcgctt
    14521 tggaagcatc gtaaccgaag cccaaagtca gatggtgttt cgtccatttg tttttcagcg
    14581 acttctcaaa cgaggcattc aaaacattgt gctgttcgcg gtagtggaaa cggtcgctgc
    14641 tgtcgtagga atacggtttg tccgccgacg cgcggcagga tttgtccacg gcaggataca
    14701 cggcgcaatt cagcttcagc gtgttgttgt cggttgccac gccctgtttg tcaaacgaca
    14761 acaccgcctt atccgcccag ttgtccgagt atttctcatt ttcataacgg tacagcaaac
    14821 ccatacggcg gcggcggtga tgttcgtcaa taaatttggt gcgggaatat ttcaaaccta
    14881 tgcccttgac caaattttta tcgcccttcc actcttctat attcggcaca aaatacaagc
    14941 cgtcgcggaa atcgtcgccg tcgtacaccc cgctcttgtc tctaaacttt tccgcctcgt
    15001 ccgtaccgta atactgtttt tccgtcatat cgcggatgtc gtaacgctgt ttggtatcct
    15061 caaacacgcc gccgacataa tgcctgccgc cgaagcggta gcccagcttg gcaagccaag
    15121 agccgctgcg gtaatccatc ggatcgggca atatcctgcc gccgcccgtg taggcttggg
    15181 cggacaggtt ttcgtggcgc gcctgcgcct cccgcacctg cgcctcttct tcagcactta
    15241 aaggctgatt ttgttcaata cgttctttta cccagcggtt gagctggcgg ttcaaatata
    15301 tcccgtagcc cgccaatttt gccacgggct tgagtgtatt ctcgccctct actaagaaaa
    15361 atggctctgt tgtcttgcgt ttaatatcgt atgtctggcg gaacgcgtcc aaacggtcta
    15421 tgccgtattc caccccgtcc gcaatatcgc cgtgcgggcg cgtttcccgc ccttggcgtt
    15481 cggttcggat tagcagccct tcccaaccgt ctttgctgaa ccccgcgccg agcgacttca
    15541 taaattggcg gtttttactg ccgtaagcgg tttttgcctg tatcccccgg cttttgccgt
    15601 ctgaaatcag gtctgccgcc tctttggtgc ggaaggcgac cgcgccgccg agtgcgccgc
    15661 tgccgtgatc ggacgaaccg gcgcctttgt cgatttccac cgtgctgatg ttttcatatt
    15721 cgatttcgtt gattgcgccg ctgccgccgc gtccgccgta tccgctcaac gatccctgca
    15781 cggtaaacgc ctgtatttgg gcaacgccgt caaccgaaac cgccacacgg tttttatcta
    15841 cgccgcgtat cgagtagccg ccgctcgcgc cgttgccctg ttcgacaacc gccacgcccg
    15901 gatcgtagcg cgtcaggtcg cggataccga gtacctgttc tttgttcaac gtttccgacg
    15961 ttttgacgat tttgcccaaa cctgtcgcct ctttcgatcg ccgtcccact ttggcggcac
    16021 ggacggtaac ctctttcaat gattgacttt gcgccgcatc aagtgccgcc gcccccgctt
    16081 gggcagcata agccggaaaa gcggttgcaa tggccaacgc cgtcaaagtc agcggaaaac
    16141 cgtgtttctt attcattttt ccacctcttt attatctttc ttcgcaccga ataccacgcc
    16201 gaattggggt ttaacttcag gttctaactg ttcgccaaca tcagcttcat tttcaacttc
    16261 attttcaata tcttcagtta taccaagaga tttcccatcc ttattgaaaa tagtgccgcc
    16321 caattccgcc gcctgcgggc cgtaaaagcc gcccgttaca agaagattgt cggctttgaa
    16381 actttgagga ttagtcgaac catttcccga aagattgatg ccgttatccc gagtgcgtgc
    16441 tgttgcgtgg aaaccgttgc cctcaatcac gccgttttca atacggaaag caggttctac
    16501 accgttttgc tccgtcagcg ttccggaaat cgatttattg ccgaaatcaa cgtcaaattc
    16561 tgcttttgcc gctttatccg ccttattatc ccattgaatg ggttcgccga tacgcgcttc
    16621 ccaagtgccg gtataatgcg ccgttccggt ttttggaatg tcggcttccg ccgtgcggat
    16681 acctttcagg aaaaggtcga tgtccctgcc tttaagggct tccggggcgg gcgggatgcc
    16741 gtctgaaaca ccgttgcctt cttccggcga ttcttcttcg ggttcttcct cttcagcttc
    16801 ttcttcgatt tcttcagctt catcagcttc ttcttcgacg atttcgtctt cgtcttcgcc
    16861 gttatcatct tcggaaactt cgtcttcgct ttcttcggcg atttcttcgt cttcgctttc
    16921 ttcaccgtta ttaatacccg aatcctcttc gtcctgcgcc ttcggtttga cggcggggcg
    16981 ttcggttttt atccgtccga gtttcacata ggtcaaaaag tcgcaacaag cactgacggt
    17041 cattttcctg ccgtcggcaa gatcgatggt tttctctttg ctaaccaaag gaatttcacg
    17101 cccttcgaca agaagtttgt caggatgacc aaaatcgggc atagtggaaa cctcaaacgg
    17161 acggggattt tcatcagtcg cctcgtcaac ggaaattttt agagaatcca agattttggt
    17221 gtgttttcca aacggcatag caggatttgt gcctgatgcg tttgtttttt gtttgcctgc
    17281 gaatacgccg aatacgccgt tgtcgttact gataaaccgt ccggcaagct cttctccgtt
    17341 atcgccgaaa aaaccgccct caagccgctg atcggcatcg gtatggaaaa acaaatattc
    17401 tttatcagcg tgtctcgtct tcacctcggt gttaactttg gcactgccgg taaagcggtt
    17461 gccgtttaaa tcggcagtga tgttgtaaat ggtcagcggt tttttaggtt cctttggatc
    17521 atttctcttt tgcacatact gatttttaat cagctcgccc tttaggattt ttttgtcgaa
    17581 atcaaccgta tattcggcag gatgtttttc cctgtcgtcg gcatccttag ccgcataaga
    17641 agttgcacca atatcgttac cataaaatgt tatataaccc aaatcgtcac tggcaaccgc
    17701 cttacctgtc cgatgacgtt tggcatcggt catatattgc cagttgccga aatattttac
    17761 cgttcccgcg ctcggtaaag attgggaagg acgttctccg gaataatata caaaaccgtc
    17821 ataaccaaaa cggtgggtaa accgcttacc acctgagttc tgttcgattt catcttttcc
    17881 gtttttagta tatacataac cggcatccac aaatttataa ttatattttt tattttcctt
    17941 ttctgacgtc ctaatttcta catcggaatc acgtttatga atttcgcttt taagttgttg
    18001 aagtttattt ccttttgaac cgtataaaaa cagaacatca ccttctttaa acgacagctt
    18061 ttcttctgct tgtttgctat cgggaattgc cgtgccatct tcacgatgaa aagcagtatt
    18121 ccgcctcaac aaccgcattg ccgcaccgac ggcgggccgg ttgaccggcg tggtttctac
    18181 cgaaggctcg gcaggggggg aagtgggaac gtccttagac ttgaaagtga cggggtacgc
    18241 ggtcggcgtt gattcgacaa caggctgcac gccgaaattg ccgccgcagg atgccagcat
    18301 caacggcagc aaggcaatac cgtaataatt cagtttacgc atccctatcc cccctattta
    18361 attaataata attatcatta tattaatatg cacagataat atcaacccgt ttttaacaaa
    18421 gatattcccg attgaaacaa aatgccgtcc gaaacctgtt cagacggcat ttgcgtaaag
    18481 ttcagcaaga aaaactacaa acccagctcg cgcaggaagc gaatgtcgtc tgcccaaccg
    18541 gatttgactt tgacccagac cttcaaaaat actttgttat caaacagttt ttccatatcc
    18601 agccgcgctt cggtggaaat ttttttcaaa cgctccccgc ctttaccgat caaaatcgcc
    18661 ttttggcttt ctttgtcgac caaaacggcg atgtagatgc ggttcaaacc gtctccctct
    18721 tcaaactgct ccacttcgac gttcatcgca taaggcagct cctcgcccaa atagcggaag
    18781 agtttttcac gcacgatttc catcgccaaa aaacgcgccg atttgtccgt aaccatgtct
    18841 tcgggataca tcggtacgct ttcgggcaga tacggcttga gcagctccaa caggttggca
    18901 atccgcaaac cgtgtttcgc actgaccgcc tccgccgccg caaattcaaa ttcggcgcgc
    18961 acttgggcaa caaacgcctc cagcgcgtaa cggtctttcg ccttgtcctt gtcgattttg
    19021 ttgatcacta aaatgaccgg cgtgtgcttg ggcagttgtt tcaacacgac gcggtcggca
    19081 tcggtaaggc gcatcgcctc cacgacgaaa accaccacat ccacaccgcc gagcgcctcg
    19141 gtaacatttt gattcagcct gtcgttgagc gcgttgcggt ggtcggtttg aaagcccggc
    19201 gtatcgacaa acacgaactg cgcggtatcg tcggtataaa tccccgttac gcggttgcgc
    19261 gtcgtctgcg cctttttgct ggtaatactg attttctgac cgatgagatg gttcatcagc
    19321 gttgatttgc ccacgttcgg acgaccgaca atcgccacga agccgcaacg gtatccgccg
    19381 gcggcgcgtt cccctgcaag gaaggtttca atatccatat tcgctttcat atccacattt
    19441 cagacggcat attcacgcgc cgcctcattt ctttttcttc ttcagcggca gcttctcttc
    19501 cagccatttc aaagcctctt tcgccgcctc ctgttccgcc gccttgcggc tcgtcccttt
    19561 ggcacggcac acgaaaccca gttcgcccaa atcgcaggaa atgacaaaca tactgtcgtt
    19621 ggcatggccg atttgctctt cgatgcggta tttcggcaag gcgaaacggc gcgcctgcaa
    19681 cgcctcctgc aaagcagttt tgccgtcttt tgcctgattt tggaaatcgg cgcgccggac
    19741 gcgttctgca aacaaatggc gcaccacctt ttccgccgtg ttgaaatcgg catcgaagct
    19801 gacggcggca aacatcgcct ccatcgcgtc cgccagtatc gaaggccgtc tgaagccgcc
    19861 gctcttcaac tcgcccgccc ccaaatacag accgtcgccg acattcattt ccgccgccat
    19921 ttccgccagc acgccctcat tgaccagact cgccctcaac cgcgacaact cgccctcggt
    19981 caacttcgga aacgcgtcaa acagcatccg cgccaccgta taattcaaaa tcgaatcgcc
    20041 gacaaactcg aaccgttcgt tgtgcttcgc atgatggctc ctgtgggtca aagcccgccg
    20101 caaaagcgac atatcgcgga acgcgtagcc taactttttc tgtatcgcag tatgtgcctg
    20161 ccgtttcaaa acatcgtctt tcattaccgt cttccgcaaa aaacagcccg aatgcgtttc
    20221 catactccaa aaatacagaa acaccttaag gctgcaaacg gcgcacattg taccaccaaa
    20281 ccgccgccgt ctgccgacaa cgcgggaatc gtggtaaaat ccgcaagttt cccccccaac
    20341 gtattcccgc aatgaacgca tttgcacaag cactttcctc ggcactcgac cgctgcatcg
    20401 ccaccaacac cgtcgccaaa caaaaccggc ccgtcggctt cctttaccgc gaagcccccg
    20461 tttttgaaaa cgacagcggc tggcgtttct tcagcggcga cgaaaccgac gaatacaccg
    20521 acgaccccga caacttcagc atcgtcagcc tcgccgacat caccaaaacc aaccctgaaa
    20581 ccgccgcact gctttcccaa cccgaaggca gcgcgtggga gcttgccgaa gacggcacat
    20641 tccaaaccgt tgccgactgg cagccgcaag actgaaacct tcagacggca ttccgaaacc
    20701 ccgaatacca aaggacaccc aatgaacacc atcgccccca acctcgacgg caaacacctc
    20761 cgcatcggca tcgtacaggc acgcttcacc aacgaaatcg gcagccaaat gctcaaagtc
    20821 tgctgccgca ccctccaaga attgggcgta gcagacgaaa acatcaccgt cgccaccgta
    20881 cccggcgcgc ttgaaatccc catcgcgctg atgaactttg cctcttccga aaaatttgac
    20941 gcactgattg ccatcggcgt cgtcatccgt ggcgaaacct accatttcga gctggttgcc
    21001 aacgaatccg gcgcagggat cggccgcgtc gcactcgact acaacatccc gattgccaac
    21061 gccgtcctga ccaccgaaaa cgacgcgcag gcaattgaac ggattggaga aaaagcctcg
    21121 gatgccgcca aagtcgccgt agaatgcgcc aacctcgtca accttctgct cgaagaacag
    21181 tttgaagacg aagaataaca aacagggcgt acaccgatgc gccctttccg tttcagacgg
    21241 cataccgtgc cgcccaaccg tttcaaggaa aaatcatgaa aacagcccgc cgccgttccc
    21301 gcgagcttgc cgtacaagcc gtttaccaat cccttatcaa ccgcaccgcc gcgcccgaaa
    21361 ttgctaaaaa catccgcgaa atgtccgact ttgccaaagc ggacgaagaa ttgttcaaca
    21421 aactcttctt cggcacacaa accaacgcag cggactacat ccaaaaaatc cgcccgctgc
    21481 tcgacaggga cgaaaaagac ctcaacccca tcgaacgcgc cgttttgctg accgcctgcc
    21541 acgagctttc cgctatgccc gaaacgccct accccgtcat tatcaacgaa gccatcgaag
    21601 ttaccaaaac cttcggcggc acggacgggc acaaattcgt caacggcatc ctcgacaaac
    21661 tcgccgccca aatccgccca gacgaaccca aacgccgttg atactcggaa aatccgaatc
    21721 ggaaaataca caaatgccgt ctgaaaacct ttcagacggc attttgcttg accgttcaat
    21781 caatactgca ccgtccagcc gatttcgccg cccgcgcgca tcgggacaag ttcgccgtcg
    21841 ccgtaaggga cgcttgcggg aacggtttgg ctttgtttga caagggtaat cgtgtcggcg
    21901 ttttccggaa tgccgtagaa ccttgcgccg ttttccgagg cgaaggcttc gagtttgtcc
    21961 aacgcgcctg ctttttcaaa cacttcggcg taaagctcga tagcggtcat cgcgctgaac
    22021 ataccggcgc agccgcaggc gttttctttg gcggatttgg cgtgcggcgc ggagtcggtg
    22081 ccgaggaaga atttgtgcgc cttctcgccg gtaacggcgg cgaccaatgc ctgacggtgg
    22141 gtttcgcgct tgaggacggg caggcagaaa tgatgggggc gcacaccgcc gaccaagagg
    22201 tcgttgcggt tgagcaggag gtgttgcggg gtcacggagg cggcaacgtt gtcgcccgct
    22261 tccaaaacga ggcgggcggc ttcggcggtg gtgatgtgtt cgaacacgac tttgagattc
    22321 ggcacttgcg ccaaaacggg tttcatcacg cgcccgataa aggcggcttc gcggtcgaaa
    22381 atgtcgattt cggggtcggt tacttcgccg tgaacgagga acaggatgcc ctgttttgcc
    22441 atttcttcca acacggggat gagcttgaac aggtcggtta cgccggaatc ggaattggtg
    22501 gtcgcgcctg cggggtagag tttgaaggcg acgatgccgg cggctttggc ttcgcgcacg
    22561 agttcgggcg tggcttggtc ggtcaggtaa agcgtcatca gcggctcaaa cgcgctgcct
    22621 tcgggcaacg ccgccataat gcgcgctttg taggcaagcg cgtcggctac gctgacgact
    22681 ggcggtttga ggttgggcat gatgacggcg cgccccatct ggcgggcggt ataaggggca
    22741 acggctttga gcgcgtcgcc gtcgcgcagg tgcaggtgca tatcgtcggg tcggatgatg
    22801 gtcagggttt gcatggttgt tcctttttag atttttggtt tccagacggc atgccgactt
    22861 tatgccgtct gaagtcaggg gagaattagg ggtttcccgt tatgggaacg gtgcgttagg
    22921 ttcaatactg cctgtacggg taaatccggg gaatcggcgg tttgtgcggt caatacggtt
    22981 tcttcagggc tgcccgattc ccgccaggca tagctgattt gcctggtttc aaacggtgcc
    23041 tgcaccgact gttccgccct caatt
//
LOCUS       DAXDXW010000027        22914 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXDXW010000027 DAXDXW010000000
VERSION     DAXDXW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22914
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.027"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(142..1530)
                     /locus_tag="ACFB64_000930"
     CDS             complement(142..1530)
                     /locus_tag="ACFB64_000930"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC1209309.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGCDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLAPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGTSFGYQTRALSKPVDLIVATPGRLTDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPADRQTLLFSATWDGAVGKLARKLTKDSEIIEVERVDGQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLTDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPARKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDSFKKTDEGFKGKRKAGDS
                     FAGKSERRYKDR"
     gene            1858..2805
                     /locus_tag="ACFB64_000931"
     CDS             1858..2805
                     /locus_tag="ACFB64_000931"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC1209310.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPGEYEAWLAEVLEAVAFVTGFEPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVIAENGRKFWVNLDKYLDTGFFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDMERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMPGILDIQRDHQKLIDGAVNLLASDGILYFSNNLRSF
                     VLDDSVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            complement(2857..3189)
                     /gene="trxA"
                     /locus_tag="ACFB64_000932"
     CDS             complement(2857..3189)
                     /gene="trxA"
                     /locus_tag="ACFB64_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC1209311.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            3487..3960
                     /gene="dnrN"
                     /locus_tag="ACFB64_000933"
     CDS             3487..3960
                     /gene="dnrN"
                     /locus_tag="ACFB64_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HFC1209312.1"
                     /translation="MTDFSVWEAAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLADMRDELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTGNFHAPEGACGSWTRLYALAKEMADDLNDHIHLENDILFARVLDS"
     gene            complement(4031..4852)
                     /gene="nadE"
                     /locus_tag="ACFB64_000934"
     CDS             complement(4031..4852)
                     /gene="nadE"
                     /locus_tag="ACFB64_000934"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC1209313.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAAQANAKGFVVGVSGGI
                     DSAVVSTLAARTGRTTLLLDMPIRQHPGQLERARRHIRNLQGQYANVSAQTANLTDTF
                     QTFEQTVGVHQTAFANQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKVEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRRREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            5049..6404
                     /gene="xseA"
                     /locus_tag="ACFB64_000935"
     CDS             5049..6404
                     /gene="xseA"
                     /locus_tag="ACFB64_000935"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC1209314.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAQPLKEGDHIEVAGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPAAVQGAGSGFQIAQAIKTASQRAECDVLIVCRGGGSIEDLRAFNE
                     EPVVRAIEACTIPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLVQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMTQNLRA
                     HTARFERQTQALQHCRPDVSVYRQDIVRLQTALPAAFSRLLARRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFSDGETDVRVSKEQGQQDLFDC
                     I"
     gene            6697..8238
                     /locus_tag="ACFB64_000936"
     CDS             6697..8238
                     /locus_tag="ACFB64_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC1209315.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYVGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLFYLALMMFGAFTIRVPADGWK
                     PEGYTVPKTQNKPVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSAGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIVGLLCNLAVKSVHEKHH
                     EKDIKTAARSGNPDDETAISDAYLVGEKVSGDGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(8297..9346)
                     /locus_tag="ACFB64_000937"
     CDS             complement(8297..9346)
                     /locus_tag="ACFB64_000937"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC1209316.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETRKTAAQAY
                     GQKASDGIKLQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTTEQMRSLTEEGVMLEDGLAKVERIYEQGGEGANKWY
                     NIVIKEGRNREVRRIFESQGLTVSRLVRVAFGPIGLPNRLKRGQFYELNPAEVANILK
                     WADMLLPGERRRKKA"
     gene            complement(9355..9507)
                     /locus_tag="ACFB64_000938"
     CDS             complement(9355..9507)
                     /locus_tag="ACFB64_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209317.1"
                     /translation="MHKLCPQSRSDDFIRKTAASGTISNRPTEDGILSTRPAAHAAEE
                     PPYQAS"
     gene            9567..10199
                     /gene="pdxH"
                     /locus_tag="ACFB64_000939"
     CDS             9567..10199
                     /gene="pdxH"
                     /locus_tag="ACFB64_000939"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC1209318.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAEVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLELNPFAAMTFFWPELE
                     RQVRVEGRVGRLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            complement(10329..11339)
                     /locus_tag="ACFB64_000940"
     CDS             complement(10329..11339)
                     /locus_tag="ACFB64_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFC1209319.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLSAAARQNLNLPHSCKNGVCGQC
                     KAELASGDIQMGGHSEQALSEAEKAQGKILMCRTTAQSDININIPGCKADALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILPATDTGYAP
                     IRSILLDLIRQNSSRAAHFYWGARHQDDLYALEEAQGLACRLKNTCFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(11431..12861)
                     /gene="gatB"
                     /locus_tag="ACFB64_000941"
     CDS             complement(11431..12861)
                     /gene="gatB"
                     /locus_tag="ACFB64_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC1209320.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGGDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRIDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLDQAINYEAEAQIEILEDGGTVQQATMLFDPEKGETRVMRLKEDAHDYGYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRVQAAYFEEAAK
                     ESGQGKPTANWMNGELAATLNKEGMELADSPITAPRLAALVGKIADGTLSGKLAKKAF
                     EAMWAEPETSIAEIIEKHSLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKTSKGKANPAQVQELIKAKLA"
     gene            complement(12904..13836)
                     /locus_tag="ACFB64_000942"
     CDS             complement(12904..13836)
                     /locus_tag="ACFB64_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC1209321.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQDAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDVLC
                     QMEDCRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPSGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAALKLDGAIGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDDMVTKMKDSLKTEARGISDGIQTEKPLRRTV"
     gene            complement(13833..15278)
                     /gene="gatA"
                     /locus_tag="ACFB64_000943"
     CDS             complement(13833..15278)
                     /gene="gatA"
                     /locus_tag="ACFB64_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC1209322.1"
                     /translation="MTQYTLKQAGSLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFVSPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSFEREKEDYTRDLDKPLKGVKIGLPKEYFSEGNSTDVQTA
                     LQNTIDLLKAQGAEPVEVSLPQTKLSIPAYYVLASAEAGTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPSAAPKIGADTSPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            complement(15341..15631)
                     /gene="gatC"
                     /locus_tag="ACFB64_000944"
     CDS             complement(15341..15631)
                     /gene="gatC"
                     /locus_tag="ACFB64_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC1209323.1"
                     /translation="MALTLADVDKIARLSRLQLTAEEKEKSLQELNDIFTMVEQMQNI
                     NTDGIEPMAHPHEVALRLREDEVTETDRAAEYQAVAPEVRNRLYIVPQVIEE"
     gene            15781..16434
                     /locus_tag="ACFB64_000945"
     CDS             15781..16434
                     /locus_tag="ACFB64_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC1209324.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGALLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            16439..17782
                     /locus_tag="ACFB64_000946"
     CDS             16439..17782
                     /locus_tag="ACFB64_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC1209325.1"
                     /translation="MFHSVNVFTGETLYRRPDQDYAEFERRLADLKMHGRAFAQLGVT
                     ERAARLQKFAGRLEAEKERFAEMVCEEVGRCLHECRAEIVKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSRALFDLASDGIPLAGAWLDEADTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFIQLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKVPQGSGWFY
                     PATVLDRVNPACRVWREEVFGPVALILRAENEEHAICLANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(17831..18259)
                     /locus_tag="ACFB64_000947"
     CDS             complement(17831..18259)
                     /locus_tag="ACFB64_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC1209326.1"
                     /translation="MNFALSVITFTLASFLPVPPAGTAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTLQTKPAVKPKPAVDTNADSAKENEKDIAEKNGQLEEEKKKIAETERQ
                     NKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            18407..18547
                     /locus_tag="ACFB64_000948"
     CDS             18407..18547
                     /locus_tag="ACFB64_000948"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209327.1"
                     /translation="MEYGYATVYFGKEKENCRQARIKSDEARDSAFGVESVESAVFDI
                     FN"
     gene            complement(18605..19861)
                     /locus_tag="ACFB64_000949"
     CDS             complement(18605..19861)
                     /locus_tag="ACFB64_000949"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC1209328.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFRKHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFFG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWSEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESPDAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQGEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERIGSEHDTRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVAKLLEQQH
                     SILDAASKLVKPQGCLVYATCSVLPEENELQIKRFLSEHPEFEPVNCAELLQNLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(19897..20766)
                     /locus_tag="ACFB64_000950"
     CDS             complement(19897..20766)
                     /locus_tag="ACFB64_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC1209329.1"
                     /translation="MTDIRISMNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELS
                     VRELLGERGFRYLLALDADPAPLTDYLAQRAPFDHRLGIYAEELLAFWFTNAPHTKLH
                     AYNLTVFSDGQTLGAADFVVSLNQQPYHIELACKYYGGDQVQNLRGLNPKDTLTDKAA
                     KLVQQFQLVHTSQGKATLAAQDLPENPLPASIVRGIGFFPQGFHAFEPPLNPYGWRGI
                     YIQDWAEYGFERQEARYHLLDRMVYLAPARVAETETLNATEIRRIDQGLIAVLECRPD
                     GFWHEIERIMKAV"
     gene            complement(20807..21622)
                     /locus_tag="ACFB64_000951"
     CDS             complement(20807..21622)
                     /locus_tag="ACFB64_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC1209330.1"
                     /translation="MTALKPALPDYLGNIRIILTRTSHPANIGSAARAMKTMGLHRLT
                     IVTPNLMATPMTENPPVFNPDDVQSFALPEESFILASGAADVLHNAEIVATLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVPELLQAANRGEKVALVFGNETFGLSIEEVRACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTDSPMTHLQQEDHAATHEQIKGMLAHME
                     SVMDDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            21866..22651
                     /locus_tag="ACFB64_000952"
     CDS             21866..22651
                     /locus_tag="ACFB64_000952"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC1209331.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGFHGKATAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDAWLESGDIVAANPKVLAQMLKIISAHV"
BASE COUNT         4650 a         5807 c         6746 g         5711 t
ORIGIN      
        1 gtaccggttt aaatttaatc cactatattg cagattaatc ctttgggtat caaagccggg
       61 cggtgctgaa acattacaaa aatgccgtct gaaactcgaa atcggtttca gacggcattg
      121 gtttacgcgg caggttgggg cttagcggtc tttgtaacgg cgttcgcttt tgcctgcaaa
      181 agagtcgccg gctttgcgtt tgcctttgaa gccttcgtcg gtttttttga aactgtcttt
      241 cttaaagcct tctttcttga aaccttcgcc gcgcgttttg ccgccgaagc cttctttgcc
      301 cggtttatga tcgccgcgcc ggccgccgga tttcctatcg ccccagccgc ctttgccttt
      361 cggcttgccg cctgcggatt tgcgtttgcg ggccggctcc atgccttcga tggtcagttc
      421 gggcagtttg cggttaatgt atttttcgat tttgtggact ttgacgtatt cgttcacttc
      481 ggcaaacgta atcgcaatac ccgtgcggcc tgcgcggccg gtgcgcccga tgcggtggac
      541 gtagtcttcc gcctgtttcg gcaggtcgta gtttatgacg tgggtaatgg tcggtacgtc
      601 aataccgcgt gcggcaacgt cggtggcaac caaaattttg cagcggcctt tacgcaaatc
      661 cgtcagcgtg cggttgcgcc agccctgcgg catatcgccg tgcaggcagt tggcggcgaa
      721 acctttttcg tacaattcat ccgcgatgac ttcggtcatc gctttggtgg acgtgaaaat
      781 cacacattgg tcgatgttgg catcgcgcag gatgtggtcg agcaggcggt ttttgtggcg
      841 catatcgtcg cagtacaaca actgctcttc gattttgcct tgaccgtcca cgcgttcgac
      901 ttcgataatt tcagagtctt tggtcagttt gcgcgccagt ttgccgactg cgccgtccca
      961 agtggcggag aacaataaag tctgacggtc ggccggggtg gcttccacga tggtttcgat
     1021 gtcgtcgata aagcccatat ccaacatacg gtcggcttcg tccaaaatca gcacttccaa
     1081 acgttcaaaa tcaactttgc cgctttgcat caggtccgtc agacggcccg gagtggcgac
     1141 aatcaaatcg accggtttgc tcagggcgcg ggtttggtag ccgaacgatg tgccgccgac
     1201 gatgctgacg gtgcggaacc agcgcatatt tttggcatac gccagtgcgt ttttctcgac
     1261 ttgcgccgcc agttcgcggg tcggtgccaa caccagcgcg cgcgggcctt tgcccggttt
     1321 ttcgctgcgt ttggtcagtc gctgcaaagt cggtaacaga aaggctgcgg tttttccgga
     1381 gccggtttgc gccgaagcca tgatgtcgca gccttccaaa gcaaacggaa tagcttgcgc
     1441 ctgaatcggc gtcgggcttt cgtaaccctc gctgctgacg gcggacaaaa tgtttttatc
     1501 aaggttcaaa tcggcaaatt taatagacat ggctatcctt ggaagacaac aacgcccgcg
     1561 tccgaaaggt ttagacggcc tgaagcaggg aagggtaacg atacggggaa tgtgaagtta
     1621 cagggttgtc gcaagcggct tgcttgtggt ttacatcgac ggcaacctgc acagatacgc
     1681 tttgcaagat caaagcaaca aaagaaacgc gcccgtaagg gcggtttagg ttggaaggcg
     1741 atggcttcgt atgacgatat aaacggagaa tggtataggg tttgcccgaa aaggtcaagc
     1801 aatcgggcgt taattacagt aaaatttccg ttttccactt tattccgcct gaaatcaatg
     1861 accgacatca caccgtttgc caaccgcttg ggcaaaaaca tcaaacacct tatgaaatgg
     1921 gcgaaacgca acggcatcga agcttggcgc atttacgacc gcgacatccc ccaattccct
     1981 tttgccgccg atgtttacgg cgatcgaatc caccttcagg aatacgatac cggctggctg
     2041 atgcggcccg gagaatacga agcgtggctt gccgaagtat tggaagccgt cgctttcgtt
     2101 accggttttg aacccgaaca aatccgtctc aaacgccgcg aacgccaaaa aggtttgcag
     2161 cagtacgaga aaaccggcaa ggcgggcgac gatttcgtca ttgccgaaaa cggccgcaag
     2221 ttttgggtca acctcgacaa atacctcgac accggcttct tcctcgatca ccgcaacacg
     2281 cgcaaaaaag tcggcgaaac ggcggcgggc aaacgttttt tgaacctgtt ttcctacacc
     2341 ggcagcttca ccgtctatgc cgccaccggc ggcgcggcat ccagcgaaac cgtcgatttg
     2401 tccaacactt atctcgattg ggcgaggcgc aattttgaat tgaacggcat cgatatggaa
     2461 cggcacaaaa tcgtccgcgc cgacgtgttc caataccttc agacggcata tggggaaggc
     2521 aggcagttcg acctcattgt gatggacccg cccagttttt ccaacagcaa gaaaatgccc
     2581 ggcatcctcg acatccagcg cgaccatcaa aaactcatag acggcgcggt gaacctgctc
     2641 gcttcagacg gcattttgta tttttccaac aacctgcgca gcttcgtttt ggacgattcg
     2701 gtatcggaac aatacgccgt gaaagacatt tccaaacaat ccgttcccga agatttccgc
     2761 aacaaaaaaa tccaccggtg ttgggaaatc cgccacaaat cttaagggtg cgaaaaagcc
     2821 gccggaatct tccggcggct tttcgattgc actttatcag gcgatggagg cttcgacaaa
     2881 ggcggtcagc tgaccttttg ccaatgcgcc gactttggtg gcgacgactt cgccgttttt
     2941 gaacaccatc agggtcggaa tgccgcgcac gccgaaacgg gacggggtgg cttcgttgtc
     3001 gtcgatgttg attttgacta ctttcagacg gccttcaaat tcggcggcaa tgtcgtccaa
     3061 aatcggggca atcattttgc aggggccgca ccacggagcc caaaagtcca gcaggacggg
     3121 gaggtcggat ttcaaaacat cttgctcgaa ggcggcatcg ccggtgtgta cgattaattc
     3181 gctgctcatt atttttcctt ttcggttggg attcgcacga tagcgtgcag gatagggctt
     3241 gggcataaaa atttcaagtg ggcgggcggg ataatagttt ttctgtaccg ccccaatcgc
     3301 ctttgcaaat tctaaaaaac ggttcggaca gtattttatt tttgaaaatt acttttatta
     3361 tcaaagtata aggaatgttt ttgaggctgt acagaaacgc gcgggcggtg tatgctccgt
     3421 ttaaatagat tcttaaagta tgaatgttca aaaccaaacc cgaaacccta taaaaggaac
     3481 actgcaatga ctgatttttc cgtttgggaa gctgctccct ttggcgcaac cgttgatcat
     3541 atcctgcaac gctaccacaa tgtccaccgc gcacaatttg aagaattggt gccgctggcg
     3601 cagaaagtgg cccaagtcca tgccgatacc tttccggcgg aaattgccgg gctgcttgcc
     3661 gatatgcggg acgagctgct gatgcatatg atgaaggaag agcggatgct gtttccgatg
     3721 attaatcagg gtgtcggacg cggtgcggcg atgcccatca gcgtgatgat gcacgaacat
     3781 gaagaacacg accgcgccat cgcacggctc aaagagctga ccggtaattt ccacgcaccc
     3841 gaaggggctt gcggaagttg gacgcgcctt tacgcgctgg caaaagaaat ggcggacgat
     3901 ttgaacgacc acattcattt ggaaaacgat attctgtttg cccgcgtact cgattcttaa
     3961 gggataggag tcggatattt caatgccgtc tgaaatacgg tttttccgtt tcagacggca
     4021 ttttcatgtt tcagcccagc aattcgggcg gaatgcggca cacaggaatc gggttgattt
     4081 tgtgctgcat cgcgcggtga agccgcgtat agatttccag aacttcgcgc cgccgtcctt
     4141 caaaatcttc gggtttgcgc gtgccgtaca cgcccattgc ccactccagt tcgggatagc
     4201 ttgcgcccat ctgttcctcg tcggtgcgtt ccgtatccca caggccgtcg gtcggcgggg
     4261 ctttttgaat cgcctcgtcc acgcccaatg cttcggcaag ccggtaaacc tgcgttttgg
     4321 tcaggtcggc aatcgggctg atgtccacgc cgccgtcgcc gtatttggta aaaaaaccca
     4381 cgccgaaatc ttcaacctta ttacctgtcc ccgtaaccag cagtccgtgt atctgcccgt
     4441 agtagtacag ggtcagcata cgcaggcggc ttctggcgtt ggcgagcgaa agcggctgat
     4501 tggcaaatgc cgtctgatga acgccgacgg tttgctcaaa ggtttggaag gtgtcggtca
     4561 gattggccgt ttgcgcgctt acattggcat attgcccttg cagattgcgg atgtgccgcc
     4621 ttgcccgctc gagctggccg gggtgttggc gtatcggcat atccagaagc agcgtggtgc
     4681 ggccggtgcg ggcggcgagc gtggagacga cggcggaatc gatgccgccg gaaacgccta
     4741 cgacgaaccc ttttgcattt gcctgagcgg cgtattcgtc caaccatcgg acaatatgtg
     4801 tgataactgc ctgcgtatcc atttttcctc ctgtcgaaaa tatccgcatc atagcctgcc
     4861 ggcggtgcaa tttgaataga ttgagggcgg gttgatgaat atttttccca ttccccgctt
     4921 aaaaaagtca tataggaaaa ggctggcggc aagatggcgc gtgttataat attgttgaca
     4981 atttagacgg cacgatgccg ccttgggctt caaaacatat tcatattttt tattatttga
     5041 ttgattcgat gtctgatttt tttcattctg acgtgttgtc cgtttccgag ttgaacgcat
     5101 ttgccaaaag tattttggaa aaccaccttg ccggcctgtg gattgcgggc gaagtgtcca
     5161 atctgacccg cgccgccagc gggcattatt atttctcact caaagacagc cgcgcgcagg
     5221 tgcgttgcgc gatgttcaag ggtgcggcgg cgcgtttggc gcaacctttg aaagaaggcg
     5281 accatatcga agtggcgggc aaaatcagta tttacgaggc gcggggcgaa tttcagatta
     5341 ccgtaaacga agtacggctc aaaggtttgg ggcagcttta cgaagcctac gagcggttga
     5401 aggcgcagtt gcaggcggaa ggcgcgtttg cggcggaacg caaaaaacct ttgcccgtcc
     5461 gtccgcaatg tatcggcatc gttaccagtc tggcggcggc ggctttgcgc gatgtcgtaa
     5521 ccaccttgaa acgccgcgcg cccgaaatcc ccgtcatcgt ttatccggcc gccgttcagg
     5581 gcgcgggcag cgggtttcag attgcccaag cgattaaaac cgcatcgcaa cgcgccgaat
     5641 gcgatgtgct gattgtctgt cgcggcggcg gcagcatcga agacttgcgg gcgtttaacg
     5701 aagaaccggt cgtgcgcgcc atcgaagcct gcacgattcc cgtcgtcagc ggcgtgggac
     5761 acgaaaccga tttcacgctc gccgatttcg ttgccgacgt gcgcgcgccc acgccgaccg
     5821 gcgcggcgga actggtcagc cccaaccgcc aagaatcact gcaccgcctc gtccaagccc
     5881 aaggccgtct gaaaaccgtt ttggagcaac gctatttcga tgccagccaa aaactcgact
     5941 ggctcgcgcg gcaaatccgc cacccgcgcc aaaaacttga cgagcagcgc gcttcaatcg
     6001 gcaagctggc gcaaacgctg tcttactcga tgacgcaaaa cctccgcgcc cacaccgccc
     6061 gttttgaacg ccaaacccaa gccctgcaac attgccgtcc tgatgtttcc gtttaccggc
     6121 aagatatcgt ccgccttcag acggcattgc ccgccgcctt cagccggctg ctcgcccgtc
     6181 gccgtcaaag cctgaccgcc caagccgcat tgctcgaagc cgtttcgccg cagcacattc
     6241 tggaacgcgg cttctccgtt gtcaaaaata cacgcggaca agtcatccgt aacgccgatg
     6301 ttttgaagca ggggcagaag ctgcacatta ccttctccga cggcgaaacc gacgtgcgcg
     6361 taagcaaaga acaagggcag caggatttgt tcgattgcat ttaaacccaa tcggaaaaac
     6421 cgctccccct ccgccgtcat tcccgcgaaa gcgggaaccc agtcccttga ccttaagata
     6481 atctttaatt cgtcattcct gcaaaagcgg gaatccggaa gcggaaagcc gcaggaattt
     6541 gtcggaaaaa acagaaacct ctccgcacac ccgaaatgtt tcagacggcg catcttgggg
     6601 cggataaaaa cccaacccga tattttttta taatttttaa taagtttcca ttgggcaaat
     6661 gaaattttat ttttcaactg acagaaagag gatgttatga agtttttaga ccgtgaggca
     6721 agcattgcca agccgggttt caaccgttgg ctcgtaccgc ccgcagcatt agcggtgcac
     6781 cttgccatcg ggcagattta cgcctattcc gtattcaacg cgccgctgac caaacttatc
     6841 ggcataaccg aatcggcggc cggagattgg aagctgacga ccgtaggttg gattttcagt
     6901 atcgcactcg cgatgctggg cgcgtcggcc gccctgttcg gcacatggat ggaacgggta
     6961 gggccgcgca aagccatatt tgccgccgcc tgctgtttca gcctgggctt cttcgtatcc
     7021 gcattcggcg tgcgtacgca caacctcttc ctgctttatt tgggcaacgg cgtcatcggc
     7081 ggcgtaggct tgggtttggg ctacgtcggg ccagtttcca cgctgatgaa atggtttccc
     7141 gacaagccgg gtatggcgac cggtttggcg attatgggtt tcggcggcgg cgcaatgctg
     7201 gcctcgccgc tttccgtatc actgatgaac gccttttcaa acgccgcttc ggtcggggtt
     7261 gccgaaacct ttgccgtatt gggcctgttt tacctcgcac tgatgatgtt cggcgcgttt
     7321 accatccgcg tacctgccga cggctggaaa cctgaaggct ataccgtgcc gaaaacgcaa
     7381 aacaagccgg tcagcagcaa tcatgtcaac gtgtcccaag ccatgaaaac gccgcagttt
     7441 tggctgttgt tctgggtatt gtgcctgaac gtaaccgccg gcatcggcgt attgggccag
     7501 gcatccgtga tgattcagga actcttttcc gaaacctccg caggcaggca ggcggcagtc
     7561 ggcgcaggtg cggcggcagg cttcgtcagc ctcctgagcc tgtttaatat gggcggacgc
     7621 tttttgtggt cgagcgtttc cgacaaaatc ggacgcaaaa acacctacac catcttcttc
     7681 gtactcggtt cgctgctgta tttcgccgtt ccctccatcg gcgaaggcgg cagcaaagcc
     7741 ctgttcatca tcggcttctg cgtgattatt tccatgtacg gcggcggatt tgccgccatt
     7801 cccgcctacc tgaaagacct gttcggcacc tatcaggtcg gcgcgattca cgggcgcatc
     7861 ctgctggcct ggtcaaccgc cgccgtcatc ggcccggtac tggtcaatta catacgccaa
     7921 agccaaatcg acagcggcat accggcggca caggcataca gcgtaaccat gtacattatg
     7981 gcgggattgt tgattgtcgg actgttgtgc aacctcgccg tcaaatccgt acacgaaaaa
     8041 caccacgaaa aagacatcaa aaccgccgca cgcagcggca accccgacga cgaaaccgcc
     8101 atatccgacg cttaccttgt gggtgaaaaa gtttccggcg acggcatatc ggtctggtgg
     8161 cgttgggcat tggccgtcat accgctcgcc tacggcgtgg tgatggtctt tgtcaaagca
     8221 ctcgaccttt tctcctgaaa acgcccgaat aaaatgccgt ctgaaacagc aggtgtttca
     8281 gacggcattt ttactttcag gcttttttgc ggcggcgttc gcccggcagc agcatatccg
     8341 cccatttgag gatgttggcg acttcggcgg ggttgagttc gtagaactgt ccgcgtttga
     8401 ggcggttggg cagtccgatg gggccgaagg cgacgcgcac gaggcggctg actgtgaggc
     8461 cttggctttc gaatatgcgg cgcacttcgc ggttgcggcc ttctttaatc acgatgttgt
     8521 accatttgtt tgcgccttcg ccgccttgtt cgtagatgcg ttcgactttt gccaagccgt
     8581 cttcgagcat cacgccttct tcggtgagac tgcgcatttg ttcggtagtc agcccgccca
     8641 gtacgcgcac ggcgtattcg cgttcgactt cgaagctggg gtgggcgaaa cgttggacga
     8701 gttcgccgga ggtagtgaga atcagaagtc cgctggtgtt gatgtccaag cgtccgatgg
     8761 cgacccagcg gctgctggcg gcctgcggca ggcggtcgaa tatgctgacg cggccttgcg
     8821 ggtcgtcgcg ggaaacgatt tcgccttctt gtttgtaata caggatgatg cgcggcaggc
     8881 ggtcggccca tttgagtttg atgatgctgc ctttgacggt aacgtggtcg tcgggggtaa
     8941 ctttgtcgcc cagttgcgcg gttttgccgt tgaccgttac ccagccgttg ttgatccatt
     9001 cttccatttc gcggcgcgag ccgacgccgg aggctgcaag cactttttgc aggcgttcgg
     9061 gttccatacg cgacaggtcg ctgcggcgtt ctttcaaatc gcgcgcgtgt tccataattt
     9121 tttggttggg attgcggacg acgagttttt tggctttggc ggcgcgctgt ttgggggcgt
     9181 tttgcagctt gatgccgtct gaagcttttt gtccgtaagc ttgggcggcg gttttgcgcg
     9241 tttcgtcttt gggacgggct ttgcttttga aaggtttggc ggttttcttg gcagacgggg
     9301 ctgcgccgtc gcgccattgg cgtttgctgg tgggttgctt ggacatcatt tttcctaact
     9361 cgcctgataa ggcggttctt ctgcggcgtg tgcggcagga cgggttgata aaatgccgtc
     9421 ctcggtcgga cggttcgata ttgtacctga tgcggcggtt ttgcggatga aatcgtcgct
     9481 gcggctttgg ggacaaagtt tgtgcaacgg ttgaaatcgg tttgcgtgcg ggagtagaat
     9541 ctttcaaatg ataagaggag cggtaaatgg atttgcacaa tattcgggaa gattacagca
     9601 aacgggaatt gtcggaagcc gattgcgccg ataatccgat cgagcagttc gagcggtggt
     9661 tggacgaggc ggtacgcgcc gaggtcaacg agccgacggc ggtcaatgtg gcggcggtcg
     9721 acgggcgcgg gaggcccaac agccgtatgg tgcttttgaa ggaggttaat tcagaaggtt
     9781 ttgttttctt tacgaattac cacagccgca aggggcgttc gctggaactc aatccgtttg
     9841 cggcgatgac gtttttttgg ccggagctgg agcgtcaggt gcgcgtggaa gggcgtgtcg
     9901 gaaggctggc ggagaagttg tcggacgaat attttgagag tcgcccgtat cagagccgtt
     9961 tgggtgcgtg ggcaagcgcg cagagcgagg tgattccgaa caaggcggta ttggtggcaa
    10021 aggcggcggc ggtcggactc aaacatcctt tgcacgtgcc gcgtccgccg cattggggcg
    10081 gctatattgt gattcccgat ttgattgagt tttggcaggg caggccgagc aggctgcacg
    10141 accgcatcca ataccgtttg ctggatggcg gctggatacg cgagcggttg tcgccttgaa
    10201 caggaaaaaa catatgccgt ctgaaaaccg gaatcaggtt tcagacggca ttttttcacg
    10261 gggaaaatac ggacgggaag ttttttgtgt tccgaacgga aagcccgaat cctctttata
    10321 ccgaggaatt atgatgcgga cggcgtgaat gcgtcggaga aaaacaagtt ttccggcagc
    10381 ttatgctgtt gcacaaacag attttttgct tgttcggtca tagccggaga gccgcaggca
    10441 aacacttcgt attccgacag gtcggggtgg tcttgtgccg cgatgtcttg tacgtggcct
    10501 tttcttccct gccagccctc tccgggacgg gacaatacgg gggtgaagca ggtgtttttc
    10561 agacggcatg ccaacccttg tgcttcttcg agggcataca aatcatcttg atgacgcgcg
    10621 ccccagtaga aatgggcagc gcggctgctg ttttggcgga taaggtcgag caggatgctg
    10681 cggatggggg cgtagcctgt gtcggttgcc ggcaggatga cgggtttgcc gctgtcttcc
    10741 tgcaaggtaa acgaaccgag cgggccttta acgcggacga tgcctttttc tttgactttg
    10801 ggttcgctgc cgaaaatcat ttccgagcag acaccgtttt cgcgcctgcg gatgtgtagt
    10861 tccaaaatgc cttcttggtc gggcgaattg gcgatggagt agctgcggct gacgttgccc
    10921 ggcagcagta aatcaatgta ttgcccggcg tagaaggcaa acggcggggc tttgggcagg
    10981 gcaagtttca ggagggcgac atcgtgtttg aaaaccatac tttcgatgcg tgcgggcagg
    11041 gtgcggacgg gtagggcatc ggctttgcag ccggggatgt tgatgttgat atcgctttgc
    11101 gcggtggtgc ggcacatcaa aatcttgcct tgcgcttttt ctgcttcgga taaagcctgt
    11161 tccgagtgtc cgcccatttg aatatcgccg ctggccagtt cggctttgca ttgtccgcag
    11221 acaccgtttt tgcaggaatg gggcagattg aggttttgac gggcggcagc ggacaaaacg
    11281 gtttcgccgt cgccggcggc aaaggtggtt tggtcgggca gggtaacggt gtggttcatg
    11341 gaatattgtt ttttatgctg ttttaatgtg gtcagactgt gaaaatgccg tctgaacgtc
    11401 ggatttgggt ttcagacggc atcttgctta tcaggcaagt ttggctttaa tcaactcctg
    11461 cacctgcgcg ggattggctt tgcctttgct ggtcttcatc acttgcccca caatcgcatt
    11521 cagggctttt tcgttgccgg atttaaactg ttccacggct ttggcgttgt ttgccagcac
    11581 ttcgtccacc atcgcttcaa ccgcgccggt atcggtcatc tgttgcaaac tgtgtttttc
    11641 gatgatttcg gcaatgctgg tttccggttc cgcccacatg gcttcaaagg cttttttcgc
    11701 cagtttgccg cttaatgtgc catcggcaat tttgcccacc agcgcggcaa ggcgtggggc
    11761 ggtaatcggg ctgtcggcaa gttccatgcc ttctttgttc agcgtagcgg caagttcgcc
    11821 gttcatccag ttggcagtcg gcttgccttg tccgctctct ttggcggctt cttcaaaata
    11881 ggcagcctgc acgcggctgg cggtgagcag gcgcgcgtcg tattcggaca cgccgtaatc
    11941 cgccacgaaa cgcgccgcca tttctttcgg cagctcgggc atttctgctt tggctttttg
    12001 catttgggca tctgaaatga tgacgggcag caaatcggga tcggggaagt agccgtagtc
    12061 gtgcgcgtct tctttcaggc gcatcacgcg ggtttcgcct ttttccgggt cgaacagcat
    12121 cgtcgcctgc tgaactgtgc cgccgtcttc caaaatctcg atttgcgctt cggcttcgta
    12181 attaatcgcc tgatccaaga aacggaagga attgaggttt ttaatttcgc ggcgcgtgcc
    12241 gaattccgct tggcctttcg ggcgaacgga tacgttggca tcgatgcgga acgaaccttc
    12301 cgccatattg ccgtcgcaaa tgtccagcca ggttaccaag ctgtgtaagg ctttggcgta
    12361 ggcaacggct tcggcggcgg agcgcatttc gggttcggat accacttcca acagcggcgt
    12421 gccggcgcgg ttcaggtcga tgccggtcgc gccgttcaag ccctcgtgca cggatttgcc
    12481 tgcgtcttct tccatatggg cgcgggttac gttgatggtt ttcacatcgc cgccgaccac
    12541 gatttccaat ttgccgtgtt cgacaatcgg caaatccaac tggctgattt gatagccttt
    12601 cggcaagtcg ggatagaagt agtttttgcg gtcgaacacg tttttccggt tgatttttgc
    12661 atccaaagcc aaacctaatt tgatggcttt ttcaacgact tcgcggttca ttaccggcag
    12721 tacgcccggc agcgcgcatt ccactacgct ggcgtgcgcg ttgggttctg cgccgaatgc
    12781 ggtcgatgca ccgctgaaga ttttggattt ggtgttcaat tggacgtgga tttccaagcc
    12841 gattacagtt tcccaagtca taggaggttc tttctctaaa atatcaattc gatagtcttc
    12901 gcatcagact gtccggcgga gcggtttttc cgtttggatg ccgtctgaaa tgccgcgtgc
    12961 ttctgttttc agactgtcct tcatctttgt aaccatatcg tccagcattt cataacgctt
    13021 gcggtacatc gaacgctttt tgctttcgat gtcggcaagg tctttgcggg cgggggtttg
    13081 gggaagatgc cagtagccgt cccgttccaa acttgcgccg tattcctgcc agaatgcgtc
    13141 gtaattcatt ttgacgcgct ttttaagttt ccagcgtaat ttgacttgat gtttttgtgc
    13201 aatgcctatc gcgccgtcta attttaatgc ggcggcgaaa tattgcagtg cggttaccat
    13261 cagttgttgg ggacgtaagc cgtgaagttg tttggttatg cggcggacgg tgtctttggc
    13321 ttcttcacct gacggccctt gtacggaggc tgtcagcagg tgtgtgccga caaaggcgaa
    13381 ggtcgccata tacagccggt tgcctgcttc atcgcgcaaa gataaagacc acgcgccttc
    13441 ttcgacgcag ttgtcgttgc ggttgagcca caagctgatg ccgtctgaaa ggtgcgccaa
    13501 gaccagatgg aagcggcagt cttccatttg gcacagaacg tctgtgccga agagtttttc
    13561 cgccatgaga aaatctgccg tcatcgcatc taaacggcgt ttgcagttaa aacgcctgtc
    13621 aacaaattcg cgcagcagcg gataggcatc ctgcgggcag tggaggaaca gcccctgacg
    13681 gataggggag gcgttgacaa actgctcgaa ggcgtaaatt tctttttttc cgaaaaattt
    13741 tcgcaatgtg tattttaaat gttgcagccg atataaaggt gcatagctgt aaacggtgcg
    13801 aaaatcgggg aaggtaaagg tgcggttctg cttcattcgg gtcgtttgcc gtgccaatcg
    13861 ctgttgagtt ggatttgatg cgccgcaccg aggattttgg cttcggcgaa gtagttgccg
    13921 acaagctgta caccgacggg cagtccgccg ccgctgaagc ctgcgggcag ggtcaatgcg
    13981 ggcagtccgg cgaggttgac ggcgatggtg taaatgtcgc tcaagtaggt ttcaaccgga
    14041 gaagtatccg ctccgatttt gggtgcggcg gagggtgcgg tcggcgcgag gatgaggtcg
    14101 caccgtgcaa atgccgtctg aaagtcatcg gcaacgaggc ggcgcagttt ttgggctttg
    14161 agataatagg catcgtagta gccgtgcgac agtacgtaag tgccgatcat gatgcggcgt
    14221 ttgacttcgc tgccaaaacc ttcggcgcgg gttttgccgt acatttcttc caaatcgccg
    14281 aattgggcgg cacggtgtcc gtaacgtacg ccgtcgtaac gtgaaaggtt ggtgccggct
    14341 tctgcggagg cgaggacgta gtaggcgggg atggacagct tggtttgcgg cagggaaact
    14401 tcgaccggtt ccgcgccttg tgctttcagc aaatcaatgg tgttttgcaa tgccgtctga
    14461 acatcggtgc tgttgccttc gctgaagtat tctttgggca ggccgatttt cacgcctttg
    14521 agcggtttgt ccaaatcgcg ggtgtagtct tctttttcgc gttcgaagct ggtggagtct
    14581 ttggggtcga aacctgccat tgcattcaac agaatcgcgc agtcttcggc ggtttgcgcc
    14641 atcgggccgg cttgatcgaa gctggaggcg taggcgacca taccgaagcg ggaaaccgtg
    14701 ccgtaggtag gtttgatgcc ggtaatgccg cagtgcgatg cgggttggcg gatagagccg
    14761 cccgtgtccg aaccgagcgc ggcaggggcg aggcgcgcgg caacgacggc ggcggaaccg
    14821 cctgacgaac cgccgggtac gtgttccgga ttccacgggt ttttggccgc gccgtagaat
    14881 gagttttcat tggtcgaacc catagcgaac tcgtccatgt tggtgcggcc gagcgttacc
    14941 ataccttcgt cgagcaggtt ttggacgacg gtggccgtgt agggggagac gaagttgtcg
    15001 agcattttgg acgcgcacgc gctgcgccag ccggtttggc agaaaatgtc tttgtaggcg
    15061 acgggaacgc cggtaagtgc cgaagcgttg ccctgtgcga tgcgttcgtc ggcggcacgg
    15121 gcttcggcga gggttttatc ttggtcgatg gtgatgtagc cgttgagggc gggatttttt
    15181 tcggcaatgg cggcaaggta tgcgcttgcc agttcgacgg cggaaatctg tttggactgc
    15241 aacaggctgc ctgcctgttt caatgtgtat tgggtcataa atcttccatc cttaaagggc
    15301 ttcgggcggt attgctgatg ccgtctgaag cgtattcgga ttattcttcg ataacttgcg
    15361 gtacgatgta caggcggttg cgtacttccg gcgcaacggc ttggtattcg gcggcgcggt
    15421 cggtttcggt tacttcgtct tcacgcaggc ggagggcgac ctcgtgcggg tgcgccatcg
    15481 gctcgatgcc gtctgtgttg atgttctgca tctgttcgac catagtgaaa atgtcgttta
    15541 attcttgaag cgatttttct ttttcttccg cagtcagctg cagtcgggag agtcgggcga
    15601 ttttgtctac gtcggcaagt gtcagtgcca tgaaaagcat ccttgtctga tggtaaagat
    15661 accgtcgttc gggtatgatg gggcgtattg cttggatttt taattataac ggaaaaatcc
    15721 gttgcggcgg caatttgcag catcggatgc ctgccgcacg ggtttgaaag aggggaaata
    15781 atgggattgg gtatggaaat cggcaagctg attgtggctt ttttggtgct gatcaatccg
    15841 tttagcgcgt tgtcgcttta ccttgacctg accaacggac acagcacgaa ggagcgcagg
    15901 aaggtcgcgc ggacggccgc cgtcgccgtg tttgccgtga ttgcggtatt tgcgctgatc
    15961 ggcggtgcgc tattgaaggt tttgggcatc agcgtcggtt cgtttcaggt cggcggcggg
    16021 attttggtgc tgctgatcgc catttcgatg atgaacggca acgacaatcc cgccaagcag
    16081 aatctcggcg cgcagccgga aacggggcaa gcgcgccccg cccgcaatgc aggggcgatt
    16141 gccgtcgtgc ccatcgccat accgatcacc atcggtccgg gcggtatttc gaccgtgatt
    16201 atttacgctt cggcagccaa aacgtacagc gatatcgcgc tgattatcgc ggccggtttg
    16261 gtggtcagtg cgatttgtta tgccatttta atcgttgccg ggaaggtcag ccgcctgctg
    16321 ggcgcgacgg ggctgacgat tttaaaccgc attatgggta tgatgctggc ggcggtatcg
    16381 gtggagatta ttgtgtcggg actgaaaacg atattcccgc aactggcagg ttgattcgat
    16441 gtttcacagt gtcaatgtat ttacgggcga aacgctttac cgccgccccg atcaagatta
    16501 tgcggagttt gaacggcggc tggcggattt gaaaatgcac ggcagggcgt tcgcgcaatt
    16561 gggcgtaacc gaacgtgccg cgcgtctgca aaaatttgcc ggccgtttgg aggcggaaaa
    16621 agagcgtttt gcggaaatgg tgtgcgaaga agtcggacgc tgcctgcacg aatgccgtgc
    16681 cgaaatcgtc aagtctatcg aactgatacg ctattacgcc cgcctcgccc ccgaactgct
    16741 cgcccacaaa accattgcga cgcaggcgag tttgagtcag gtgcgcttcg agcctttggg
    16801 cgtggtgttt gccgtcatgc cttggaacta tcccgtctgg caggtattgc gttttgccgt
    16861 tcccgctatg tgcgcgggca acgcgtgcgc cgtcaaaccc gcgcccagcg tggcacgcgt
    16921 cagtcgggcg ttgttcgatt tggcttcaga cggtattcct ctggcaggcg cgtggctgga
    16981 tgaggcggac acgctcaagg cagtcgaaga taccgatgcg atggcgttta ccggttcgac
    17041 gcatacgggg cgcattcttg cggcacatgc gggcgcaaac ctcaagaaaa ccgtgttgga
    17101 gctcggcggc agcaacgcct ttatcgtgat gcccgatgcc gatttggaac gcgccgccgc
    17161 agaagcctgt tattcccgtt tccgcgatgc ggggcaatcg tgtaacgccg ccaagcgcat
    17221 catcgtaacc gaagccgcag ccgaccgctt tattcagctg tttctcgccg aatgcgccaa
    17281 attgaaaatg ggcgacccga aacatcccga taccacgctt gcaccgctgc accgcgaaga
    17341 tttgcgggac cgggttcacg ggcaggttga agatgccgtt tcaaacggcg cggtgtgcct
    17401 gaccggaggc aaagttccac aggggtcggg gtggttttac cctgcgacgg ttttagacag
    17461 ggtaaatcct gcctgccgcg tctggagaga agaagtattc ggccccgtcg ccctgatttt
    17521 gcgcgccgaa aacgaagaac acgccatctg ccttgccaat gattccccgt tcgggctcgg
    17581 tgcctgcatt tataccgccg atactgaacg cgcctggcgg tttgccgaaa aaatacaggc
    17641 cggatcggtg tttatcaacc gccataccag cagcgatttg cgcctgcctt tcggcggagt
    17701 caaagattcc ggttacgggc gcgaactgtc cgaattcggg ttgtacgagt tcgtcaacgt
    17761 caaaacctac tggcagaaat aaacaaaccc cgtgccgctt caggcggcat cgggtttgcg
    17821 ctagagccga ttaacggcag tatttgttta cggcgttatt gtatttacgg atcaaatcat
    17881 ccttgttttt cgcatttgag tttcccaccg ccttcaggtt catttttgaa atccggcagt
    17941 tttcttcttt gttctgccgt tcggtttcgg caattttttt cttttcttcc tcaagctgcc
    18001 cgtttttctc ggcgatatcc ttttcgtttt ccttcgcact gtccgcattc gtatcgacgg
    18061 caggtttggg cttgaccgcc ggtttggttt ggagcgtccg caggttgagg atttggctct
    18121 ggtcgggatg aagctgtttc ggcacatccg aatagctgtt gccgccgccg tctttccaag
    18181 taaagacggc ggttccggca ggcgggacgg gcaggaaaga ggcgagggta aatgtgatga
    18241 cggataaagc aaagttcatt agggtttcct tgatggctga ctgcccgggc agaatcggga
    18301 ttggttcggg cggtgtcttt tccgggttcg ggtcaaggca gggaagacac tccgccttaa
    18361 tatggtgctt tatatattgg ggtaaaacag attcccaaaa atatcattgg aatatggtta
    18421 tgctaccgta tattttggca aagaaaaaga aaattgcagg caggcgcgta taaaatccga
    18481 tgaggcgcgt gattctgcct ttggtgttga atcggttgaa tctgcggttt ttgatatttt
    18541 taattgatga aatgccgtcc gaacgctttg aaaagggttc tgacggcatt ttgttcaaac
    18601 cggtttattt gcgttgcaat acggcggcga agaagccgtc ggtttggtgt cgggcggaat
    18661 cgaggcgcag gtatttgccg gtatccaaat cgaccttcag gttttgcagc agttcggcgc
    18721 agttgacggg ttcaaattcg ggatgttcgg acaggaaacg tttgatttgc agttcgtttt
    18781 cttccggcaa cacgctgcag gtggcgtaaa ccaaacagcc ttgcggtttc accagtttgg
    18841 aggcggcatc gaggatactg tgttgctgtt ccaaaagttt ggcgacggtc tcggcggatt
    18901 ggcggtattt gaggtcggga ttgcggcgca gtgtgcctaa gccggagcag ggcgcatcga
    18961 ccaacacgcg gtcggctttg cctgccagtc gtgcgatacg ggtatcgtgt tcgctgccga
    19021 tacgttcggg gtggatgttg gtcagtccgg cacgggtcat gcgcggtttg aggttggcaa
    19081 ggcgtttttc ggcgatgtcg aaggcgtaga ttctgccttt gttggccatt tgggcgccga
    19141 cagccaaggt tttaccgccg gctccggcac agaaatccac aatgatttcg ccgcgttttg
    19201 cgcccaccaa taaggcaagc agctggctgc cttcgccttg gacttccagc gtgccgtcca
    19261 aaaacagttc gtgtttgtta agcgcgattt tgtttttcag gcggatgccc caaggagaat
    19321 aaggcgttgc ctctgcatcg gggctttcgg cttgcaacaa tggcaatact ttgtcgcgtt
    19381 tgcctttcaa ggtattgacg cggatgtcga gcggcgcagc ttggttgatg ctgcggccga
    19441 aagcgaggat ttcttcttcg ctccaatgct gctgcagttg ttccaccagc cattgcggca
    19501 attctgcggc ggtattcagg ccgtctgaaa actcggtttt acgggctttc aaattgccga
    19561 aaaactctgt ttcttcttca tcaagcaggt ctttgatttg gctgatgttg gtacttctgc
    19621 cgagaaccag cgcggcgagt gcggctttgc gcggctgcgc gtgcggacgg cgcagggcgg
    19681 tactgatttt ttgatagtgt cgcaacgcgg caaaggcggt ttcggcgatt tcgtggcggt
    19741 cttggcggcc gagctttttg tgtttgcgga aataggcgga gaggacggca tcggcaggct
    19801 gtttgaaagt cagcatttca gccaaaactt tggcggtatg gtcgagttgt gcggcgttca
    19861 taatgtaatg ggttctctta aagttggtaa agggtttcag acggccttca taatacgttc
    19921 gatttcgtgc caaaagccgt ccggccgaca ttccaaaacg gcaatcaagc cttggtcgat
    19981 gcggcggatt tcggttgcgt ttaatgtttc ggtttcggcg acacgcgcag gcgcgagata
    20041 gaccatgcgg tcgagcaggt gatagcgcgc ttcttggcgt tcaaacccgt attccgccca
    20101 atcttgaata tagatgccgc gccagccgta tggattaagc ggcggctcaa aagcatggaa
    20161 accttgtgga aaaaatccga tgccgcgcac gatggaagca ggaagtggat tttccggcag
    20221 gtcttgtgcg gctaaggttg ctttgccttg cgacgtatgc accagctgga attgctgcac
    20281 caatttggcg gctttgtccg tcagcgtgtc tttgggattg agaccgcgca ggttctgcac
    20341 ttggtctccg ccgtagtatt tacacgccag ctcgatatga tagggctgtt ggttgaggga
    20401 aacgacaaaa tccgcggcgc ctaaagtttg gccgtctgaa aaaacggtca gattatatgc
    20461 gtgcagtttg gtgtgcggtg cgttggtaaa ccaaaaagcc agcagctctt cggcataaat
    20521 gccgagacgg tggtcgaacg gggcgcgttg tgcaaggtaa tccgtcagcg gcgcgggatc
    20581 ggcatccaat gctaaaaggt agcggaaacc gcgttccccc agtagttcgc gcacgctcaa
    20641 ttcgcaaccg ctttgccaca aaggcggcgc agtcagcagc gaggcgaggt cgcggacggg
    20701 ttggctggtg agtttccacc ataatgcgtc tagggcgtaa ttcatgctta tccgaatatc
    20761 agtcatgccg tctgaaaaag cttgtaatgt tttcagacgg ccttaatcag tcttttttat
    20821 ggatacggtg gctgacggta ttgaaaaaac cgcgcaggat gtcgatgtct tcggtttgcg
    20881 tgttggcgcg tccgaacagg ctctgcatac ggcgcatcag acgctcgccg ttgcggcggt
    20941 tgaaaaagcc gatgtcgtcc atcacgcttt ccatgtgggc gagcatgcct ttgatttgct
    21001 cgtgggttgc ggcgtggtct tcctgttgaa gatgggtcat gggcgaatcg gtttggctga
    21061 aaatttcgta gcacacgacc tgcacggctt gggcgaggtt gagcgagaaa tagtcggggt
    21121 tgccgttgat ggtcatcagt cggttgcacg ctcggacttc ttcgatgctc aagccgaaag
    21181 tctcgttgcc gaagaccagc gccactttct cgccgcggtt ggcggcctgc agtaattcgg
    21241 gcaccaaatc gcgcggggtt tgcagcggcg cggtaatttc gcggcggcgg ctggtcaggg
    21301 cgcaggcgat ggtggtgtcg gcaagggctt cgtccagtgt ggcgacgatt tcggcgttgt
    21361 gcaatacgtc cgccgcgccg gaagcgagga tgaaactttc ttcaggcagt gcaaaacttt
    21421 gcacgtcatc gggattaaaa acgggcggat tttccgtcat cggcgttgcc atcagattgg
    21481 gggtgacgat ggtcagcctg tgcagaccca tcgttttcat cgcgcgtgcg gcagagccga
    21541 tgttggcggg gtggctggtg cgtgtgagga tgatgcggat gttgccgagg tagtcgggca
    21601 gggcgggttt gagagcagtc atgttcggtt ttcggtcgga atggaatagg gtataatgcg
    21661 ccgtcttttt cgttcggacg gtgaaatgcc gtttgaactg ttctttaaca acgtcggaaa
    21721 tttaacgttg cgcctgaagc agacacgcgt caggcggtat tttacccgat ttggcagggt
    21781 ttgccccaac cagataaact ccgattttgc cgctccgaag gactgcacgt ccgaaccggc
    21841 ggtatgccac ccttaaggaa atctgatgaa tccgtttttg aatacagcct ttaaagccgc
    21901 ccgccgtgcc ggtcagatga tgattcgcgc cgcaggtaac ctcgatgccg tcaaaaccga
    21961 cagcaaagcc ttcaacgatt ttgtttccga tgttgaccgc aattccgaaa tcatcctggt
    22021 tgaggctttg aaagaagcct atccgcatca caaaatcact tgcgaagaaa gcggcttcca
    22081 cggcaaagct acagccgagt acgaatggat tatcgatccg ctcgacggga cgaccaattt
    22141 ccttcacggc catccccaat acgccatctc tatggcactg ctgcacaaag gcgtgttgca
    22201 ggaagctttg gtgtacgccc ccgaacgtaa cgacgtgtac atggcttcgc gcggtaaagg
    22261 cgcgttgctt aacgaccgcc gcatccgtgt ctccaaccgc atcgaattga accgctgcct
    22321 gatcggtacc ggtttccctg ttgtcgatca aagcatgatg gacaaatatc tggcgatttt
    22381 gaaagacttt ttggcaaaaa ccgccggagg ccgccgcgaa ggcgcggctt ctttggattt
    22441 gtgcgccgta gcaacaggac gttttgacgg ctttttcgag ttcaacctca aaccgtggga
    22501 cattgccgcc ggtgcattga ttgtccaaga agcaggcggc atcgttaccg atatgtcggg
    22561 tgaggacgct tggttggaaa gcggcgatat tgtggctgcc aatcccaaag tactggcgca
    22621 aatgttaaaa atcatttccg cacacgttta aatttcagcc agctcccgga aatgcgggaa
    22681 aacgggcggg tatgccgtct gaagtatcgt gccgatattt cagacggcat ttttattggc
    22741 ggcggggcag gaaggcggaa acggtcaaat gcccgaaatt gccgggaatg gaaatccgta
    22801 tctggttaaa cggagaaccc tgtgcaattt gtcccttttc tttcaattgt ttaaaggcgg
    22861 tttgctggaa tctaaatcga ctgtggcaga aaacagacac cggggaataa aaaa
//
LOCUS       DAXDXW010000028        22847 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXDXW010000028 DAXDXW010000000
VERSION     DAXDXW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22847
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.028"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(41..211)
                     /locus_tag="ACFB64_000953"
     CDS             complement(41..211)
                     /locus_tag="ACFB64_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFC1209332.1"
                     /translation="MAQYMCGPCGWIYDEELGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
     gene            complement(344..1425)
                     /locus_tag="ACFB64_000954"
                     /pseudo
     CDS             complement(344..1425)
                     /locus_tag="ACFB64_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            complement(1563..2111)
                     /locus_tag="ACFB64_000955"
     CDS             complement(1563..2111)
                     /locus_tag="ACFB64_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC1209333.1"
                     /translation="MARLTVHTLETAPEAAKPRVEAVPKNNGFIPNLIGVLANAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIRIIAARTNQCGFCVAGHTKLATLKKLLSEQSLNAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDDELKAFFDAGYNRQQAVEVVMGVALA
                     TLCNYANNLAQTEINPKLQAYA"
     gene            complement(2251..2622)
                     /locus_tag="ACFB64_000956"
     CDS             complement(2251..2622)
                     /locus_tag="ACFB64_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC1209334.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     MTGAMFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGG"
     gene            complement(2807..4498)
                     /gene="dld"
                     /locus_tag="ACFB64_000957"
     CDS             complement(2807..4498)
                     /gene="dld"
                     /locus_tag="ACFB64_000957"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC1209335.1"
                     /translation="MNASQLISSLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQKKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKFLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFSHHGGAFFECNAEETQAAMLHRFAVASAAIRYRSVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            complement(4595..5014)
                     /locus_tag="ACFB64_000958"
     CDS             complement(4595..5014)
                     /locus_tag="ACFB64_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209336.1"
                     /translation="MKKLLMITLTGMLAACSTGVNVGRLMVEMPQGERSVVVQVPATN
                     NPLSDAVAVGMIKTSGSPSASNMIEMLGADNINVGVAGGSQMFNKATALYSLNHAKKV
                     GNNVSVYMTGDSESDKADLENAANAKNIKLHYFFNQK"
     gene            complement(5237..5333)
                     /gene="ffs"
                     /locus_tag="ACFB64_000959"
     ncRNA           complement(5237..5333)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFB64_000959"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5594..9427
                     /locus_tag="ACFB64_000960"
     CDS             5594..9427
                     /locus_tag="ACFB64_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC1209337.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWHILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     QQADVLIAAAEKAVELFDGSFDETRQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAVPLDANSAVIN
                     TEKLDKHRSVEYVELAGLYGRHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTAATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVAAVKARWDWLLAHLDAPLADYKSRYGTA
                     VHAAPEAKDDESCFTAFRDFRLRVSVKADVMKPLAEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLTDEDLQPFWNYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIHNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYSDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            9504..10505
                     /locus_tag="ACFB64_000961"
     CDS             9504..10505
                     /locus_tag="ACFB64_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFC1209338.1"
                     /translation="MIDGQTTEPKQKTRIIPAPMRGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAD
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTGKMRLGYEDKS
                     PALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIHDTVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSTWIGQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEMDV"
     gene            complement(10566..10748)
                     /locus_tag="ACFB64_000962"
     CDS             complement(10566..10748)
                     /locus_tag="ACFB64_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209339.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPKAGEKRQ"
     gene            complement(10921..11052)
                     /locus_tag="ACFB64_000963"
     CDS             complement(10921..11052)
                     /locus_tag="ACFB64_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209340.1"
                     /translation="MSETAKIKLNDRPENAEKPNEKVELPIVDNDKKGGHGEGGCCG"
     gene            complement(11311..12455)
                     /locus_tag="ACFB64_000964"
                     /pseudo
     CDS             complement(11311..12455)
                     /locus_tag="ACFB64_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            complement(12780..13256)
                     /gene="greA"
                     /locus_tag="ACFB64_000965"
     CDS             complement(12780..13256)
                     /gene="greA"
                     /locus_tag="ACFB64_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC1209341.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPAEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            complement(13520..13648)
                     /locus_tag="ACFB64_000966"
     CDS             complement(13520..13648)
                     /locus_tag="ACFB64_000966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209342.1"
                     /translation="MAKRKYKDKNSLHYTGANAVWKISGLAFSVLKYVNLVFYAEN"
     gene            13647..14948
                     /gene="aroA"
                     /locus_tag="ACFB64_000967"
     CDS             13647..14948
                     /gene="aroA"
                     /locus_tag="ACFB64_000967"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC1209343.1"
                     /translation="MTESVRLPAASLKPSTVALPGSKSISNRTLLLAALSDNVCEIHS
                     LLKSDDTDRMLEALDKLGVQIEHLAEGRLKVHGTGGRFPNRSADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGNEHYPPLHIGKRQDCGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     EGYRVFKIPADAHYHAPEHLHVEGDASGASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKGRAVQAFDLDANHIPDAAMTLAIVALATRQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLISLLGVPVVINDPKCTHKTFPTYFEVFSSLTETAE"
     gene            complement(15026..15559)
                     /locus_tag="ACFB64_000968"
     CDS             complement(15026..15559)
                     /locus_tag="ACFB64_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC1209344.1"
                     /translation="MFSPDKTLFLCLGALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIGRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPGSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(15616..17142)
                     /locus_tag="ACFB64_000969"
     CDS             complement(15616..17142)
                     /locus_tag="ACFB64_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC1209345.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVLLDNI
                     LQIRHTPHNNGLSDIYLLDDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     MYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDNPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDKHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            complement(17254..18795)
                     /locus_tag="ACFB64_000970"
     CDS             complement(17254..18795)
                     /locus_tag="ACFB64_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFC1209346.1"
                     /translation="MIFDNNYHYNAMPIRLLVNIQDADSAGIQTASENNNGTRMGYNH
                     PMEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADLNESPPDMQQAVVA
                     YTLTVALLIPLSSYLADKFGTKKVFFGSIAVFMLGSALCAASGSLFELALSRVVQGIG
                     GSMLVPIPRLTILRVYEKSKLLNAINYAVMPALIGPALGPLAGGYLVEYASWHWIFLL
                     NLPIGLLGFILGRNIMPDVKGNDTALDFKGYLTFSVAACLLLLAAESLSHALPPYFAL
                     LPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFSRLGISSIPFLMPL
                     MFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLLWNTKLLAAFIMLL
                     ALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGSGNSLMAVNQQLAI
                     SVGIVAGALILKNWTFLIPASSGLHFAFRMTLLSIGGITLASSLVFKRLHVSDGANLT
                     RGTRP"
     gene            18943..19722
                     /locus_tag="ACFB64_000971"
     CDS             18943..19722
                     /locus_tag="ACFB64_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC1209347.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAIALIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKNTEYESKAVDCANRIIEFTHFLLAIGYKPEDKGEPVKVAVHTSCAARREINV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKV"
     gene            19719..20420
                     /locus_tag="ACFB64_000972"
     CDS             19719..20420
                     /locus_tag="ACFB64_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC1209348.1"
                     /translation="MSARENILAKLKKAGALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNID
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            20417..21871
                     /locus_tag="ACFB64_000973"
     CDS             20417..21871
                     /locus_tag="ACFB64_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC1209349.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDVPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(22020..22439)
                     /gene="fitB"
                     /locus_tag="ACFB64_000974"
     CDS             complement(22020..22439)
                     /gene="fitB"
                     /locus_tag="ACFB64_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin FitB"
                     /protein_id="HFC1209350.1"
                     /translation="MILLDTNVISEPLRPQPNERVVAWLDSLILEDVYLSAITVAELR
                     LGVALLLNGKKKNVLHERLEQSILPLFAGRILPFDEPVAAIYAQIRSYAKTHGKEIAA
                     ADGYIAATAKQHSLTVATRDTGSFFAADVAVFNPWHD"
     gene            complement(22439..22675)
                     /gene="fitA"
                     /locus_tag="ACFB64_000975"
     CDS             complement(22439..22675)
                     /gene="fitA"
                     /locus_tag="ACFB64_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin FitA"
                     /protein_id="HFC1209351.1"
                     /translation="MASVVIRNLSEATHNAIKFRARAAGRSTEAEIRLILDNIAKAQQ
                     TVRLGSMLASIGQEIGGVELEDVRGRNTDNEVSL"
BASE COUNT         5274 a         6110 c         6214 g         5249 t
ORIGIN      
        1 cggaacacgc cgagggattt tccaaggggt gcggctgccg ctatatcacg aaatccaaca
       61 ggtagaaatc ttctttgccc acgccgcatt cggggcattt ccagtcgtcg gggatgtctt
      121 caaactttgt cccgggggcg atgccgtgtt ccgggtcgcc gagttcttca tcgtaaatcc
      181 agccgcaggg gccgcacata tattgagcca tttgtgtttc cttgtttgta ccgtgctttt
      241 ttgcagcggg ccgccggggt ttggttcgga tgccgtctga agcgtgccgc cgcatctgtc
      301 ccgccttggg cggtttgtgc ttcagacggc attttccgcc tgtttatttt gcggcttcga
      361 ttgctgctat ttctttgcag aggtgtttga ttgcgggggt tacgatggcg acaaacatcg
      421 cttcgccgac gcgcctttgg gcgggactcc gcatcaggta gcctttcgcg ccggaatgca
      481 gggcggcgga ttgcgcagcg gcaagggcca gtacggcggc ggcttcgcgc agcttgaggg
      541 tggcgaggtt gtcgggcgtg ttgtcccacg ccaatccggc gagccgttcg gtttctgccc
      601 acgcgccgtc cagccttgct ttgaggctgt cgtagccgtc gtcgagatag ctgttgactt
      661 ctgcattgac gatgttggcg aggcggatga tgccgaggct gccgtctatc acgcccgcgc
      721 cgatgccgat ttgcaggagg ataaagcctg ctttgatgct ttggatgtag ccggcaaact
      781 gttcgggcgc ggcgatgatg tcttcgtcgg ggataaatac gtctttgaag ttcaggctga
      841 agggcgcgtg ccttcgaggg cgcaaaattc ggggcagttt tgcaggctta cgccttccct
      901 ttgcccgcct gtgatgaaca tgacgtagcc gccgccgatt tgggcggtat tcgcccaaat
      961 gtggtcttcg ccgatgttgg acacccacgg cagcgcgccg ttgactttgt aaccaccgtc
     1021 cacgcactcg gcttgaaggt tgtgtttttc aatgccggca aggtgtttga cggtattgga
     1081 cattccggtg cccgccaata ctttgccttg caggatgtcg gcgaggtatt tgtctttgac
     1141 ggcttggttg ggcgtttggt gcagatacca cgcgcaagcc gcctgacacc acgcgctgaa
     1201 ggaggtcgcg ccgcattctt tgccgatttc gcgcaagacg gcgattcgtg ttgccaagcc
     1261 taagccgttg ccgccttcgg cttctatacc gactgcgccg attgcgccga gttcgcgcat
     1321 aaatgcttcg gggtagtatc ctttgcggtc gatgtcgtcg acgaggggtt tgagcttggt
     1381 tttgacgagt tcggcaacgt tggcaagcag ggtttgggcg ttcatctttg ttccttaagg
     1441 tttttggggg gaaatcgggg gcacgcctga tgcgcggctg cttttcccgc cgcttgggcg
     1501 gcttgttttt gtttgagggg gtgtttttga aaaaccgcct gcattcaggc ggttttgttt
     1561 gcttaggcgt atgcctgcaa tttggggttg atttcggttt gggcgaggtt gttggcgtag
     1621 ttgcacaaag ttgccaaggc tacgcccatt acgacttcga ctgcctgctg ccggttgtag
     1681 cccgcatcga aaaatgcttt gagttcgtcg tcggatactg cgcctttttt cgccattacg
     1741 gcttgggtga aggcggcaag cgcgccgagt ttggcatcgt caaattcacc tgccgccaaa
     1801 gcgcgggcgg cattgaggga ttgctcggac aggagttttt tcagggttgc gagtttggtg
     1861 tgccctgcca cgcagaagcc gcattggttg gtgcgtgcgg caatgatccg gatgacttcg
     1921 acttcgccgg cggtcaggct gttggcggcg ttgagcttgc cgacttcttg gtaaaacgcc
     1981 aaggcttcgg gggcgtttgc caatacgccg atgaggttgg ggataaagcc gttgtttttg
     2041 ggtacggcct ctacgcgcgg tttggcggct tcgggggcgg tttcgagggt gtgtacggtt
     2101 aaacgtgcca ttttctttcc ttgttcgcaa atattgggga cgggcgcatg gtacgcattt
     2161 cggaacggaa taggaaagac tgtgccacaa acaaaaagtt ataagaaatg ccgcctgaag
     2221 cctttcagac ggcattgccg ggaaacacgc ctacccgccc ccgcgtccga aatattgcgc
     2281 caatgcggct tgggggttgg cggcttcgac tgtttcgggc aattcggtat agccgcgcga
     2341 taaaaggtgg cgtgcataaa ggctgttgcc ctttgcgccg aaccagacgg aaaccgccaa
     2401 gcccaatacg ttaaacgccg agtctgtggc gggcgtgtcg ccgtaaacca gttgggcaaa
     2461 caccgccaac acgaacatcg cgcccgtcat ccccagcccc aaaccccaca ttcttttgaa
     2521 acacgcccac agcgtgccga acaaaagacc cggccacgac cagccttgtt tgacggcttg
     2581 gggcggcagg gcgggatggg tgtagatttt gtatggtttc atcgtgtttc cttttcggtt
     2641 gaaaccccgc cctttggggc ggtaggatca gactttattt gtgagacgcg cgcccgtgtg
     2701 ccgacatacg gcttgaacat gcccgtcggg gaagacagat ccgacaatgc cgtctgaacg
     2761 gtgttttgcc ttcagacggc attgcctgcc ttcaaagcgg acgcgcttat tccgcccagt
     2821 ttttcttttt gctggttttg ccgatacccg ggttgaagct gttggtcggg tcgagtttgc
     2881 ggtaaaactg tttgagcgcg ggtttggctt catacaaatg gccgacgttg tgttcggcgg
     2941 gatattgcgc gccgcgttgg tctaagagat gcagcatttc gtgttccaac gccatacagt
     3001 cgttgccttt tttgatgatg taatcctgat ggaaaacgtg gcacataaaa tgcccgtaat
     3061 acaatttatg gatgatttta ttgtcgattt ccggcggcag tttttcaaac cagtcgcggt
     3121 cgtcgcggcg cagggcgata tccagcgcga ccaagtcctc cacttcgtcg tcgtgcacgg
     3181 aacggtagcg gatggcggcg gaagcgacgg cgaaacggtg cagcatcgcg gcttgggttt
     3241 cttcggcgtt gcactcgaaa aacgcgccgc cgtggtgtga aaaatattct tttaagaacg
     3301 cgcgcgcctc atccacgcct tttccgccca ttttcagaat caggtggtgt tcgtatttgt
     3361 cgcggtaatc gcgcatggat ttgggcaggt ggtcgggcag gaatttgctg acgaactgca
     3421 ttgccttgtc ggaaaaatgt ttgggcagga agctgacttt tttgccgaac ctgtccacgc
     3481 gcgccttcaa gtcaaataat ttcggcagtt ggtgcgtacc gaattttttg atgacgtaga
     3541 acgtgtcttt gccgtacacg tcggcaatgt cgaaagcgtg gcggtggatg tattcgccgg
     3601 aaacgggcag gctttcaaat tcgcccaagg cggcgcggcg gatgtcggtc agctcgttga
     3661 tgtcgttcgt gccgatgtag aacacggcgg tttgtttttc ttgcgggaag gtgtccaaac
     3721 ggacggcaaa aaccatcagc ttgcccgcgc agcccgaggc ttcgtaatgg cgggcggggt
     3781 cggcattgaa acgcgcggcg gtcggttcgt ccacttggcg gacgtgttcg caataggcgt
     3841 ggtcatgtcc tttgcccgcg tcttgtttga tgtctttttt ctgataatga tgaccttgca
     3901 ggttggtcag gatttcttcg ggcgtgtcgc ccaagtctat gcccaagtgg ttgaccagtt
     3961 ccaacctgcc ctcttcgttg atttgggcga acaacgccat ttcggtgtag gccgggccgc
     4021 gctgtaccaa cgcgccgccg gagttgttgc acacgccgcc taaaacggac gcgccgatgc
     4081 aagaagagcc gatgaccgaa tgcggttctc gtcccaaagg tttcagcagc agttcaagct
     4141 ggttcagggt cgagccgggc aggcagacga cttgttcgtt gttgttgatg gtttggatga
     4201 tgttcatccg catggtgttc acaatcacga tgtcgcggtc gtaatcgttg ccgtcggggg
     4261 tcgaaccgcc ggtcagtccg gtattcgccg cctgcgtaat cacaatcacg tccgcttcga
     4321 cgcaggcctg cagaattttc cacatttcca aaatgcttcc cgggcgcacc accgccaacg
     4381 ccttgccttc gccgaaacgg tagccttggc ggtattgttc ggttttcgcg gggtcggtga
     4441 tgatgtattt ttcgcctacg gtttgggtca ggctgctgat taattgcgag gcgttcataa
     4501 cactctccta tcgggataag cggcggcgca aatcccggta cgccgccgta tgttcaattt
     4561 ggatattttg gtgcggcaag ttgcacgctg caaattattt ttggttaaag aaataatgta
     4621 atttaatatt tttggcattt gccgcgtttt ccaaatcggc cttgtcgctt tcgctatcgc
     4681 ccgtcatata gacactgaca ttatttccga ctttctttgc atggtttaag gaataaagtg
     4741 cggtcgcctt attaaacatt tggctgcctc ccgccacgcc gacgttgata ttgtccgcgc
     4801 cgagcatttc aatcatattt gatgccgaag gcgatccgga tgttttaatc attccgacag
     4861 ccaccgcatc ggaaagcggg ttattcgtcg cgggaacctg cacgacaaca gagcgttctc
     4921 cctgcggcat ttcaaccatc aaccggccga cattgacacc tgttgaacaa gctgcaagca
     4981 taccggtgag ggttatcatt agaagttttt tcatttgcct actcctttag tctttaatca
     5041 aaccgccctg ttcgtcatat aacttgaaat tgaaccatac ttccattttt atgaagccgg
     5101 ttcttcattg gcctgtatgg atacattgta atgatgcgtt atggcaaagt aaaggaaatg
     5161 ccgtctgaac ggctttcaga cggcatcggt gcgatacggg aacgccggaa catcgaagct
     5221 ccggcgtttc aaataaggcg gcgggccaaa cccccggcac tggcgcatcg gagtgggctg
     5281 ctggcttccg cccctgaccc ggtgttccga tttgccatgc ggggagaccc gcctcagaga
     5341 aacggcatta taacgggttt tctgaaaaac tcaaccgttt tgatacggtc atacgccgga
     5401 aacaccgcct aaaatttata tttgataata ttgtcaacaa tttctcaaag cgttattttg
     5461 tttctataag ggtatttcct gtttcggcat tgaaaaatat caaaaattga actacattat
     5521 cgccttttca aacccggctg aaaccgactt ttcaaacggc attcaaataa aaactgccaa
     5581 acacggacac accatgacca cgactaccgc cccgcagcgt attcgggaaa tcccctacaa
     5641 ctatacttcc tacaccgacc gcgaaatcgt catccgcctg ctgggcgacg aggcgtggca
     5701 cattctgcag gacttgcgcg gacagcgcaa aaccgggcgt tcggcgcgga tgctgtttga
     5761 agtgttgggc gatatttggg tggtcgtacg caatccgtat ctggtcgatg atttgctgga
     5821 gcatccgaaa cgccgcgccg cgctggtgcg ggaaatgcgc cacaggttga acgaaatccg
     5881 caaacgccgc gacgataatc agcaagcgga tgtgttgatt gcggcggcgg aaaaggcagt
     5941 ggagcttttt gacggcagct ttgacgaaac gcgccaaaag cggcggcaga ttttggagcg
     6001 tttgagcaaa atcaccaagc cgcacaatat tatgtttgac gggctggcgc gggtaacaca
     6061 cgttaccgat gcgaccgact ggcgcgtcga atatccgttt gtcgtcgtca atcccgacac
     6121 ggaggcggaa gtcgcgcctt tggtgcgcgc cttaatcgag ctggatttgg tcattatccc
     6181 gcgcggcggc ggtacgggtt ataccggcgg cgcggtacct ttggacgcaa acagcgcagt
     6241 catcaatacc gaaaaactcg acaagcatcg cagcgtcgaa tacgttgagc tggcaggctt
     6301 gtacggcaga catccgatta tccggtgcgg cgcgggcgtg gttacgcggc gggtggaaga
     6361 aaccgcgcat caggcgggct tggtgttcgc cgtcgatccg acttccgccg acgcgtcctg
     6421 cgtgggtggt aatgtggcga tgaatgcggg cggtaaaaaa gccgtattgt ggggtactgc
     6481 cttggacaac ctcgcctact ggaacatggt taaccctcaa ggcgaatggc tgcgtatcga
     6541 acgcgtgcgc cacaatttcg gcaaaatcca cgacgaagaa actgccgtgt tcgacgtgca
     6601 cacgctggat tcagacggca tcaacatcgt taaaaccgaa cgcttggaaa tccccggaca
     6661 caaattccgc aaagtcggtt tgggcaaaga cgttaccgac aaatttttga gcggactgcc
     6721 cggcgtgcag aaagaaggta cggacggcat cattaccggc gttgccttcg tattgcacaa
     6781 aatgccgaaa tacacgcgca ccgtgtgtat ggagtttttc ggcacggccg ccaccgccac
     6841 gccttctatc gtcgaaatcc gcgattttct gctcgcacac gacagcgtgc ggctggcggg
     6901 tttggagcat ttggactggc gttatgtccg cgccgtcggc tacgccacca aagcggcggg
     6961 caaaggacga ccgaaaatgg tcttgctggc ggacgtggtt tcagacgacg aagccgccgt
     7021 agaggcagcc gccgaacaca tctgcgaact tgcccgcgcc cgtgacggcg aaggctttat
     7081 cgccgtatcg cccgaagcgc gcaaaacctt ttggctcgac cgcagccgca ccgccgccat
     7141 cgccaaacac accaacgcct ttaaaatcaa cgaagacgtg gtgattccgc tggagcggct
     7201 gggcgagtat tcggacggca tcgaacgcat caacatcgag ctttccatcc aaaacaaact
     7261 caaactctgt gccgctttgg agcaatatct ttcgggcaaa ctccccatcg acaaaatggg
     7321 caccgacctg ccgaccgccg aactgttggg cgaacgcggc aaacacgccc tcgcccacgt
     7381 tgccgccgtc aaagcgcgtt gggactggct gctcgcccat ttggatgcgc cgcttgccga
     7441 ctacaaatca cgctacggta cagccgtcca cgccgcgccc gaagccaaag acgatgaaag
     7501 ctgcttcacc gccttccgcg atttccgcct gcgcgtgtct gtcaaagcag acgtaatgaa
     7561 gccgcttgcc gaaatcttca gcggcaaaac cgataccaaa attatccaag gcttgggcaa
     7621 aatccacgcc aaaaccgtac gcagccgcgt ctttgtcgcc ctgcatatgc atgccggcga
     7681 cggtaacgtc cacaccaata ttccggtcaa ctcagacgat gccgaaatgc ttcagacggc
     7741 ctaccgttct gtggaacgca ttatgaaaat cgcccgttcg ctcggcggcg tgatttccgg
     7801 cgaacacggc atcggcatca ccaagctcga attcctcacc gatgaagatt tgcaaccgtt
     7861 ttggaactac aaaaaccaag tcgaccccaa gcacaccttc aaccgccaca aactgatgaa
     7921 aggttcggac ttacgcaacg cctacacgcc gtctttcgag ctgttgggcg cggaatcgct
     7981 gattatggaa aaatcagacc tcggcacgat tgccgattcc gtcaaagact gcctgcgctg
     8041 cggcaaatgt aaacccgtct gctccaccca cgttccgcgc gccaacctgc tgtacagccc
     8101 gcgcaacaaa atcctcggcg tgggtttgct gaccgaggcc ttcttatacg aagaacaaac
     8161 ccggcgcggc gtttccgtca aacattttga agaactgatg gacatcggcg accactgcac
     8221 cgtgtgccac cgctgcgtca aaccctgccc cgtcaacatc gacttcggcg acgttaccgt
     8281 agccgtccgc aactaccttg ccgattccgg ccacaaacgc tttgcgcctg cggcttcaat
     8341 gggtatggcg tttttgaacg ccaccggtcc gaaaaccatc aaagccctgc gcgccgcgat
     8401 gatacagacc ggcttccccg cgcagaactt tgcctacaaa atcggcaagc ttcttccgat
     8461 cggcacgaaa aagcaaaaag ccgaacccaa ggcaaccgtc ggcaaagccc cgattaaaga
     8521 acaggttatc catttcatca accgcccact gcccaaaagc gtacccgcca aaacaccgcg
     8581 ctccttattg ggcatcgaag acggcaaaag catccccatc atccacaacc ccgccgcgcc
     8641 cgaagatgcc gaagccgtgt tctacttccc cggctgcggt tcggagcgtt tgttcagcca
     8701 aatcggactc gccgtccaag ccatgctctg gcacgtcggc gtacaaaccg tcctgccgcc
     8761 cggctatatg tgttgcggct atccgcaaga cgctggcggc aacaaggcaa aagccgaaga
     8821 aatgagcacc aacaaccgcg tcgccttcca ccgtatggcg aacaccctca actacctcga
     8881 catcaaaacc gtcgtcgtca gttgcggcac ttgttacgac cagcttgaaa aataccgctt
     8941 cgaggaaatc ttccccggct gccgcatcat cgacatccac gaatacctgc tcgaaaaagg
     9001 cgtgaaactc gacggcgtga aaggtcagca atacctctac cacgacccct gccacacccc
     9061 gattaaaacc atgaacgcca cccaaatggc cagcagcctg atggggcaga aagtcgtttt
     9121 aagcgaccgc tgctgcggcg aatccggtat gttcgccgtc aaacgccccg acatcgccac
     9181 acaggtcaag ttccgcaaac aagaggaaat cgagaaaaac ctcaaagagc tgccgcaggg
     9241 cgaacccgtc aaaatgctga cctcctgccc cgcctgcctg caaggactga gccgctattc
     9301 cgacgacaac aatatgcctg ccgactacat cgtcattgaa atggcgaaac acatcctcgg
     9361 cgaaaactgg ctggatgagt ttgtaaaaaa agccaacaac ggcggcgtag agaaagtgtt
     9421 gctataacaa cggacacgga aatgccgtct gaacgccgaa agccttcaga cggcattgtt
     9481 tgaatcaaat acctacccga atcatgattg acggacaaac aaccgaaccc aaacaaaaaa
     9541 ccagaatcat accagccccc atgcgggggc tggtagacga tgtgatgcgc gacctgctga
     9601 cacgtatcgg cggctacgac gaatgcgtca gcgaatttgt acgcattacc cataccgtgc
     9661 attcacgatc catatggtta aaatatgtcc ccgaaatcgc caacggaaac aaaacgtttt
     9721 ccggcacgcc ctgcaccgtc caacttctgg gcagcgatgc ggacaatatg gcggcaaacg
     9781 cattggaagc cgtccgattc ggcgcggaca aaatcgattt gaacttcggc tgccccgcgc
     9841 ccaccgtcaa caaacacaaa ggcggcgcaa ttcttctgaa agagccggaa ctgatattcc
     9901 acatcgtcaa aacgctgcgc ggacgtttgc ctgcacatat tccgctgacg gggaaaatgc
     9961 ggctaggcta tgaagacaaa agccctgctt tggaatgcgc ctgtgcgatt gccgaagggg
    10021 gcgcgtgcgg actgaccgtg cacgcgcgca ccaaagccga gggctacgaa cccccggcgc
    10081 attgggaatg gataaggaaa atccacgata ccgtcaatat tcccgttacc gccaacggcg
    10141 acgttttcag cctgcaggac tatatcggca tcaaaacaat cagcggctgc aacagcgtga
    10201 tgctcggtcg cggtgcggtc atccgccccg atttggcgcg ccaaatcaag caatacgaga
    10261 acggcggacc ggtcaaagac acggattttg ccgaagtttc cacatggata gggcagtttt
    10321 tcgagctgtg cctgacaaaa gaggcaaaca acaaataccc gatcgcccgt cttaagcaat
    10381 ggctgggcat gatgaaaaaa acgttcgacc cggcgcaaac gctgttcgac cgcgtccgaa
    10441 cggttaaaga tgcggatgaa gtgcgccgaa tcctgaacgc atttgagcat gagatggatg
    10501 tttgatgtct cccgcgcttc aaagcaaatg ccgtctgaaa acctttcaga cggcatttgt
    10561 tatctttatt gccgtttttc ccctgctttc ggtgtatttt tgtttttcag tttcgccccc
    10621 aaacccaaac gtttttcata atccgattgc ggggtatcgt cttcctgcat accgaacgcg
    10681 ccggcattga cccacagcga cagcagcgcg acgacaaagg cgcaaaagcc gattacatac
    10741 caaaacattg cccctccaaa tttgttaaaa tcatatcaaa tacagtgccg aatttatcac
    10801 aagcgcacga gcaaataaat cttctgaccg cagtatttca ttcatttacc ttgaaaacaa
    10861 aaaagggcac ctgaaaattt tgcgaaactt ttcagatgcc ctgctttgcc gaaaaacgta
    10921 tcagccgcag caaccgcctt cgccgtgtcc gccttttttg tcgttatcga caatgggcag
    10981 ttcgactttt tcgtttggtt tttctgcatt ttcgggccgg tcgttgagtt tgattttcgc
    11041 ggtttcgctc atttccttac ctcatttgga aaacggtttg aaagattcat aaatatagca
    11101 tgtttaatcc gaatctgaaa agggaagccg tatgcgaaat cccgtctgaa gcccaatcag
    11161 ggcttcagac gggatttgct atctttattg ccgcttttct tccgtatccg gatttttgtt
    11221 tggggctgaa gcagattggc agtcagattg caatcgaaga atgaaggcaa gccatcaaaa
    11281 acaaagctat ccgcttcacc gccccgatat ttagaatttg tggcgcagac caacggaggc
    11341 ggcattaatt tgagtgtagt tgccgatgcc ggtatttcgt ttcagccaag cggcagacat
    11401 gatggcggaa gtgcgcttgg aaaaatcgta atcgacaccg gcgatgattt gatcatagct
    11461 ggtatgttcg cgtttctgac tgcgttcgac aaagtcgaaa ccatgggcat agctgatgcg
    11521 cgggactgta ttaccgaagc ggtaggaagc agtggcggca atttcggtcg tactgttttt
    11581 ggttttgtcg gcattttcag acaaatccaa ctgagccgcc aaggcgagat tcaagccgcc
    11641 ttccccatag ccgcccgtca ggcggtgtac ctgatggttt ttcaatggat cggtatcact
    11701 cgctctgccg agcaagaaca aaaagaaagc atcatgcccc tcattggcgt gtttcgcata
    11761 tttaagggca taatttccga aaaagccgcc atttttgtaa ttcagaccgg cataatacac
    11821 atccgacccc ggcttgccga caacagccgg aactggtttc atctgattac tttccagcgt
    11881 gaaagtagca ggcgtatagg cggacttgct gttttgactc ggaacaaatt gaatgctgcc
    11941 gctgaaaccg gaaaatccgg gggaatcgta acgcacggaa accggcatac cgtcgtggcg
    12001 tttgaaaata cccaattgcg aagccacatt attattgctg tcccaaggat caatggcttt
    12061 gctggcatcg tcaaacggat tcgcaacgcg accggcgagc gccgtgccga attcgcctgc
    12121 caagccgata aaggattccc tgttacccca acgggtcgcg ccgccgccgg caacggatac
    12181 gtcttgctcg agctgccaaa cagccttcag cccgccgccc aaatccccac ccccttaaag
    12241 ccgataaacg agccgaaatc actgactttc gtcctgatgc ggcttttggc cttagtaact
    12301 gtattgcccg tttgaccttc tgagggtggc tcggtcaact gcagccggat gttcctgcct
    12361 tccacaccag ctttgacttc gccgtacagg ctgacatcgg caactgccgc aaacggcagt
    12421 gcggacaata cgagggcggt aagttttttt cgcatatcgg cttccttttg taaatttgct
    12481 aaaaacatcg ggcaaacacc caatcagtct ccaattaccc cccgaaaaaa aaaccatttt
    12541 tcggaacaaa tttcgggtaa attttggaat ttttattttg aaaaataata tttcaatata
    12601 aaacaataaa ttaacttttt tgaaaatgtt gctttcccgc aacatgattt aaatacagac
    12661 cttgtcaaag ccgatgcggc gcagacccgg atgccgtctc gttttttaaa acaccataaa
    12721 aaaacacacg cggtacttta atttcctgcg tgtgtcgagt gtatcgaaat caagccgaat
    12781 caaatatacc ggacttcgat aatatcgtat tcgcgtacgc cgcccggtgc ctgaacttcc
    12841 gccgtatccc cttcttcctt gccgatcagg gcgcgggcaa tcggagagcc gacatagatt
    12901 ttgccctgtt tgatgtcggc ttcgtcttcg ccgacaattt gataggtaac gtgttcttcc
    12961 gtttccaaat cttccagcgt aaccgtcgta ccgaacacga ttttgccttc ggcgtggatt
    13021 tcggccggat tgatgatgtg ggcaacggaa agtttgtgtt ccaactcgga aatgcggccc
    13081 tcgataaaac cttggcgttc tttggcggct tcgtattcgg cgttttcgga caaatcgccg
    13141 tgcgatctgg cttcggcaat cgcttcgatc acttcgggac gcgccacgct tttgagctgc
    13201 tgcaattcct gtttcagcaa ttccgcaccg cgtacggtca gggggatttt ttgcatcggt
    13261 ctttttctcc atattccggc acaccggttt gcggcggcaa gcataccgcg taccgtcttg
    13321 ttttgtgcgt ccggatatta aaataaaaat acaagccgcc cggaaaatcg gcggcttgtc
    13381 tgtcgttgaa cagcggctat tctaccaaat tctatgaaat tggcaatcgt gccgtgccgc
    13441 cggcagacgc gctatgtccg caacaaaagc cgaaaatatg ccggcaaaaa aattttagaa
    13501 acaaaaaatt taaaaaaaat caattttcgg cataaaaaac caaattcacg tactttaaaa
    13561 ccgaaaatgc caaacctgag attttccaga cggcatttgc accagtataa tgcaggctgt
    13621 ttttatcttt atatttacgt tttgccatga ccgaatccgt ccgcctcccc gccgcctcgc
    13681 tcaaaccttc caccgtcgcc ctgcccggct ccaaaagcat cagcaaccgc accctgctgc
    13741 tcgccgccct gtccgacaat gtttgcgaaa tccattccct gctcaaatcc gacgataccg
    13801 accgtatgct cgaagcactc gataaactcg gcgttcaaat cgaacatctt gccgaaggcc
    13861 gtctgaaagt acacggcacg ggcggacgct tccccaaccg ctctgccgat ttgtttttgg
    13921 gcaacgcggg cacggcgttc cgtccgctga ccgccgcgct ggccgttttg ggcggcgatt
    13981 atcatctgca cggcgtgcct cgtatgcacg aacgccccat cggcgatttg gtcgatgcgt
    14041 tgcggattgc cggcgccgat gtcgaatatc tcggcaacga acactatccg ccgcttcata
    14101 tcggcaaacg tcaagactgc ggcgagcgcg tgattcctat taaaggcaat gtgtccagcc
    14161 agtttctgac cgccctcttg atggcgctgc cgctgaccgg gcaggcgttt gaaatccgta
    14221 tggtcggcga gttgatttcc aagccctata tcgacattac tttgaaactg atggcgcaat
    14281 tcggcgtaca ggttgccaat gaaggctacc gcgttttcaa aatccctgcc gatgcgcact
    14341 atcacgcacc cgaacacttg cacgtcgaag gcgatgcctc cggcgcgtcc tacttcctcg
    14401 cagccggttt gattgccgcc acgcccgtcc gcgttaccgg catcggcgca aacagcatac
    14461 agggcgatgt cgcctttgcc cgcgaactgg aaaaaatcgg tgcggacgtg gtttggggcg
    14521 aaaacttcgt cgaagtctcg cgcccgaaag gacgcgccgt ccaagccttt gatttggatg
    14581 cgaaccatat ccccgatgcc gcgatgaccc tcgccatcgt cgcgcttgcc acaaggcaaa
    14641 cctgcacgct tcgcaacatc ggttcgtggc gcgttaagga aaccgaccgc atcgccgcaa
    14701 tggcgaacga gttgcgcaaa ctcggtgcaa aagtcgtcga agaagccgaa gcgattcaca
    14761 ttaccccgcc cgaaacgccg acacccgatg ccgtcatcga cacttacgac gaccaccgca
    14821 tggcgatgtg tttctcgctg atttcgctgt tgggagtacc cgtcgtcatc aacgacccga
    14881 aatgcaccca taaaaccttc ccgacttatt ttgaagtgtt ctcatcactg accgaaacag
    14941 cggaataagg cggcaaaatg ccgtctgaaa cccgaaaaca gggcttcaga cggcattttg
    15001 ccgcgattcc ggcgcggcgg gcggctcatt ctgtaaaaaa cgtatgcgct ccaaggtagt
    15061 ttttggcgta aaacggtgtg gagagttttt cggttttgat ggttttgccg ctgccggggg
    15121 catggatgaa ttcgccgttg ccgatgtaga gtccgacgtg tgagtagcgg tgtgcgccgc
    15181 cggtgttgaa gaatacgagg tcgccggcct tgaggcggct gtcggggatt ttgcggcttg
    15241 ccgccgccat gtcgcgggcg gtgcgcggca gcttgacgtt gagggcgttt ttgtaaacga
    15301 attgaatcat gccgctgcag tcgaagccgg ttgcggtgct gctgccgccc catttgtagg
    15361 gcgtgccgat gagtccgagg ctgtggagca tgagttcctg cgagccttgt gtgcggccga
    15421 tgtggctgat gcggacggct tggatttgcc ggactgtctg tttgggtttc ggttggcggt
    15481 gtttgccgga ggtcgtgccg catgaggcga ggagcagtgc gccgagacag aggaaaaggg
    15541 ttttgtcggg ggaaaacatg gttttttcct ttgcgggttc ggatatgccg tctgaaggtg
    15601 tttcagacgg catttctata ataaaccttc gatgggcagc agggatagga tttttgcggc
    15661 gatgcgtttc caaagtttgg cttcgggttc gttcgggtag gtttttcggg tggcgggatc
    15721 gtgccattgc aggcggttgt gtttgtcgag ggtaacgcgg taggcgtatt cgggtgtggt
    15781 atcggcgagg gtgcgctcca tctgttctgc gattttgggg ctttcgatga cgacgcccat
    15841 ttcggtattg agccgtgcgg aacgggggtc gaggttgaat gagccgatga agatgcgttt
    15901 gccgtccaca atgaaggttt tggcatgcag gctggttacg gagctgccgg tcaggccttt
    15961 gtcttttgtg gcggggacgg catggttggg ttgcagctcg tagagtttga tgccggcttt
    16021 gagcagcggt tttcggtatt tgacgtagcc ggaatggacg gcggcaacgt cggtcgcctg
    16081 tagcgagttg gtcaggacgg taacgtctat gccgtcctgc accagttttg ccagtgcgtc
    16141 tgtgccggat tttgtaggga cgaaataggg tgaaaccaga tagacgcttt tttcgggctg
    16201 tttgagcgcg tcttgcagtc tcccggcaat cggcggtttg cggcggtcgc ggtcgagtcc
    16261 ttttgcaggg ttgtcgctga tcaggcgggt ttggacgctc tgccagtcga tgcgtcccgt
    16321 ctgtattttt tggtagaggg gcgactgttc gacggtttcg cggtagcgca ggagcgcgtg
    16381 tctggatgtt tcgtcgttgt atccgagtgc ttgaagaccc ttgccgatgt tgccgctgcg
    16441 gatgatgcgc gtggcgttgt gggcggaatg gcttgcccag tagcggtcga agtcgtgcga
    16501 tacttcgccg acgacgctgc cggtggcgag gatgtccagg tcggcgaaaa cggtgtcctc
    16561 accgactttg aagtattcgt cgccgatatt gcgtccgccg agtatggtgg cgcggttgtc
    16621 ggcggtaaag gatttgttgt gcatgcggcg gttgaggcgg gggaagtcgg tcaggtagcc
    16681 gagtgcgcgc catttgcgta ggacgaaggg gttgaacagg cgcacttcga tattgggatg
    16741 gctgtcgagg gcgagcagga gatcgtccaa cccgcgcgtg ttgttgtcgt ccaacagcag
    16801 gcgtacgcgc acgccgcgtt ctgcggcaag gtacatgagg ttgaacagca gcctgccgga
    16861 aatgtcgttg cgccaaatgt agtattgcaa atcgaggctg tgttcggcag attcgataag
    16921 ggcggcgcgg gcggcaaagg cttcgtgggg gtcgtcgagc aggtagatgt cggatagccc
    16981 gttgttatga ggggtgtgcc ggatttgcag gatgttgtcc aggaggacag gtttggaagt
    17041 attgaaatga cggctttccg tccgttcttc cagtgggggc aaccatgaag aacatgaaca
    17101 gagaaggagg cataaaaggg aaatgaggct gcgtgttttc atcggggata tagtttcaga
    17161 cggcattgcc tgtattttgg ggtctgtatg cattgaaatg aagcatcata atctaaaggt
    17221 tggaatgggt aaaagttttg tcgggatgcg gggttaaggc cgcgtgcctc ttgtcaggtt
    17281 tgcaccgtct gaaacgtgca gacgcttaaa aaccaacgac gaggcaaggg tgatgccgcc
    17341 gatactgagc agggtcatac ggaaggcgaa atgcaggcct gaagaagccg gtatcagaaa
    17401 tgtccagttt ttaaggatta atgcgccggc aacaatgccc acgctgatgg caagctgttg
    17461 gttgaccgcc atcaggctgt tgccgctgcc ggtctgttgc gggcgcaaat cggcgagggt
    17521 cagtgtgttc atggcagaaa actgtaggga gttgcatgcg ccgatcgcca gcgagaggaa
    17581 aatccaaatc cacagcggcg agtttccgtc aggcagggcg agcagcatga tgaaggcggc
    17641 aagcagcttg gtgttccaaa gcagtaccgt gcggtagccg aaacgtttca tgagcggtgc
    17701 aatcagcggt ttgaccagca gcgaagacag ggcgacgggt gcgaccagcc agcccgacag
    17761 gcttgcgccg aagccgaaag cgacttgaaa catcaggggc atcagaaaag gaatcgagct
    17821 gatgccgaga cggctgaaca gattgcccgc cagtcccaaa cggaaggtgc gtatcagaaa
    17881 caggtcggcg gaataaatcg gtttggacgc ggttttcata tgtcggaaat aacggcgtgc
    17941 aaacagcagt ccgccgcaca gcggtaacag tgcaaaatac ggaggcagcg cgtgcgacag
    18001 gctttctgcc gcaagcagca gcaggcaggc ggcaacggaa aaggtcagat agcctttgaa
    18061 atccagagcg gtatcgttgc ctttgacatc gggcatgatg ttgcgtccca atatgaaacc
    18121 cagcagaccg atgggcaggt tgagcaggaa aatccagtgc cacgaagcgt attcgaccag
    18181 ataaccgccc gccagaggtc ccaacgcagg gccgatcagt gcaggcatga ccgcataatt
    18241 gatggcgttg agcagcttcg atttttcata tacacgcaag atggtcagac gcggtatcgg
    18301 aaccagcatc gaaccgccga tcccctgaac gacacgggaa agcgccaatt caaacagcga
    18361 acccgatgcg gcgcacaatg ccgatccgag cataaaaacg gcaatcgaac cgaaaaagac
    18421 ttttttcgtt ccgaacttgt ccgccaaata actgctcaaa ggaatcagca gggcaaccgt
    18481 cagcgtatag gcaacgactg cctgttgcat atccggaggc gattcgttca ggtcggcggc
    18541 aatttcgggc agtgcggtat ttaaaatggt cgcatccaac atctgcataa aaatggcaat
    18601 tgccagcaga agcggcagcc aaggggatgg tgcgcgggcg gacagggtgt ttttttccat
    18661 agggtgattg taccccatcc ttgtgccgtt attgttttca gatgctgtct gaatgccggc
    18721 agagtcggca tcctgaatgt tcacaagcaa acgaatcggc attgcattgt aatgataatt
    18781 attatcgaaa atcatcagag taaggtacag taagcgttat ggggcagttt gtaagaaaaa
    18841 ccggattatt ttttaaaatt agacttgacc cgcaacggtc aattacttaa agtaaacgct
    18901 tacctttcta cagaaaaaac gggtttcccg ttatcaaaaa atatgagcgc aaccattccc
    18961 ccaaaaatca tccgatacga cagcaatccg acagatgtct attttttcgg cacttgcgtc
    19021 cttgatcttt ttatgcccga agcaggcatg gatgccattg ccctaatcga gcagcagggc
    19081 atacgcgtcc atttcccgat ggcgcaaagc tgctgcggtc agcctgccta ttcatccggc
    19141 catccgaccg aagccttcga tgtcgccaaa gcgcaactcg accttttccc cgaaaactgg
    19201 ccgattgtcg taccgtccgg ctcatgcggc ggcatgatga aacatcactg gcctaccctg
    19261 tttaaaaata cagaatacga atccaaagcg gttgattgtg ccaaccgtat cattgagttt
    19321 actcatttct tgcttgccat cggctacaag cctgaagaca aaggcgagcc agtcaaagtc
    19381 gccgttcaca cttcctgtgc cgcccgccga gaaataaatg tccatctttc aggctggcag
    19441 ttgattgacg gtatggaaaa cgtcgaacgc atcgtccacg accacgaaag cgagtgttgc
    19501 ggcttcggcg gcacattctc cgtcaaacaa gccgatattt ccggcgcgat ggtaaccgac
    19561 aaagttgccg cgctgaaaga aaccggcgca accgaaatca tcagcgcgga ctgcggctgt
    19621 atgatgaaca tcggaggcaa aatcgccaag gacgagccgg atatgccgcg tccgaagcat
    19681 atcgcatcct tcttgttgga acgcaccgga ggcaaagtat gagcgcgcgc gaaaatattt
    19741 tggcaaaact gaaaaaagcc ggcgcattgc cgatggaaga gcctgcggtt tttgattatt
    19801 accgcgaaaa gggcgtttct tgggacagcg aagccgagcg tctgaaacat tgggctgccg
    19861 ccatgcgcgc ggtcaaaacc gaaatctatt gggtgacgaa aagcaattgg atgcaggttt
    19921 tccgcgaagc ggcagaaggc aagggtttaa aaaacatcct gctgcccttg gcgaccgaac
    19981 acggacaaat tgcccgtgcc gcattggcgg gcagcaatat cgacccgatt gccttcgagc
    20041 gcgaaatcga tacttggaaa accgagtttt tcacgaacat cgatgcaggc ttcagcggcg
    20101 cgcaatgcgg catcgcccgc accggcacgc tgatgctgtt ttccagccct gaagaaccgc
    20161 gtactttgag cctcgttccg cccgtgcatt tctgcctgtt cgatacgtcc aagatgtaca
    20221 acgagtttca caacgccgtc gaaggcgaaa aactggtgga aaacggtatg ccgaccaatg
    20281 tattcctgat ttccggcccg tccaaaaccg cagacatcca gttgacgctc gcttacggtg
    20341 cgcacggtcc gcgtgatttg gtcatcctcg ccattttgcc cgaccacatt tcccctgccg
    20401 atttggagga aaacgcatga ctacgcaaac catcaagttc cacatgaagc cggaaacttt
    20461 caaacaaaac gccgcaattt cccttcaaga caagcccttg cgtaaaagcc tgcgtaccgc
    20521 gatggatatg ctgatgacca agcgcaaagc cgttttgacc gacgaagaag agttgcaaag
    20581 cctgcgcgat ttgtgcgaac acatccgtca gcgttccctc tccaaattac ccgcgctgct
    20641 ggagcagttg gaagaaaacc tgactaagtt gggcgtgaaa gtacactggg cggaaacccc
    20701 tgccgaagcc tgccaaatta tccacgacat catcacagcc aaaaacggca agctgatggt
    20761 caaaggcaaa tccatggtca gcgaagaaat cgagctgaac cattatcttg aagcaaaagg
    20821 cattaaggcg gtggaaagcg acttgggcga gtttatcgtc caaatggcag gcgaaaaacc
    20881 gacccatatc gtgatgcccg caatccataa aaccaaagaa caggtcagcg aactgttcca
    20941 ccaaaacctc ggcacaccgc ttacagacga cgtggaccaa ctgaccggat tcgcccgcaa
    21001 agccctgcgc gatatttaca gcactgccga tgtcggcttg agcggcgtaa acttcgccgt
    21061 tgccgaaacg ggcacgctgt gtctggtcga aaacgaaggc aacggccgct tgagtactac
    21121 cgttccgccc gtacatatcg ccattacggg tattgaaaaa gttgtcgcca agctgtctga
    21181 tgttccgcct ttgtacagtc tgctgccgcg ttccgccatc ggtcagaaca ttaccactta
    21241 tttcaatatg attaccggcc cgcgccgcag tgaagaatta gacggtccgc aggaaatgca
    21301 cttggttttg ctcgacaacg gccgcagcca agcttatgcc gaagaccaaa tgcgccgcac
    21361 cctgcaatgt atccgttgcg gcgcgtgtat gaaccattgc ccggtttata cacgcatcgg
    21421 cggcgcggca tacggcacaa cctatcccgg tccgattggc gagattattt ccccgcacct
    21481 gttgggtctt gatgccaccc gcgacctgcc gaccgcctgc acgatgtgcg gcgcgtgcgt
    21541 ggaagtttgt ccggtacgca tcccgattac cgaacaaatg caacgtttgc gcgttgaagc
    21601 acaacgttcg ccgaccgaaa ccgtgccgca ccccatccga gggcaaggcg catcgcatac
    21661 cttcggcgaa caaatggcgt ggcgcacatt caacggtatt ttcagcggta gcaaaaccta
    21721 ccgcgccttc ggttgggcag ccaccaagtt ccgcaatctg accccgcgca aacagttggg
    21781 ttggacgcaa aaccgcgtac cgatgaaacc ggcgaagaaa accctgcacg aactgatggc
    21841 ggaaaaaatg cgccaaaaag aacaggcata aaaagttgtt cgcaaaaata ccgtctgaaa
    21901 cccgaaaaca gggcttcaga cggcctgaaa agcagcctga acgcgcaaaa ataaataatg
    21961 aaacggaatg ctaagacgct ttcaggcagc ctgacattaa ggctgcttaa aagcgtcttt
    22021 taatcgtgcc acggattgaa caccgcgaca tcggccgcaa aaaatgagcc ggtatcacgc
    22081 gtagcaactg tcaaactgtg ctgttttgca gtggcggcaa tatagccgtc tgcggcagca
    22141 atctctttgc catgtgtttt ggcataggaa cgaatttgcg catagattgc ggcaaccggt
    22201 tcatcaaaag gcagaatccg ccccgcaaat aaaggcaaaa tggattgttc caaacgttcg
    22261 tgcagcacat tctttttctt gccattgagc aacaacgcca cacccaaacg caattctgca
    22321 acagtaatgg cagacaaata cacatcttcc aatatcaaac tatccaacca tgccaccaca
    22381 cgttcattgg gttgtgggcg caaaggttcg gaaatcacat tcgtgtccag caaaatcatc
    22441 acaaagaaac ctcgttatca gtattacgac cgcgtacgtc ttccagctca acacctccga
    22501 tttcctgccc tattgatgcc aacattgacc ccaaacgtac agtttgttgt gctttggcga
    22561 tgttatccaa aattaagcgg atttctgctt cggtactgcg ccctgcggct cgcgcacgga
    22621 atttgattgc gttgtgcgtg gcctcggata aatttctaat cacaacagaa gccattgcct
    22681 ttccttcctg aaaaaagttt ttcaaatgct atcaaaataa aaaaaatgat agcaatctat
    22741 tttttaacgc ctgcggatgc ctgaaaattc aggtggccgc aggcgttttt ttacatctaa
    22801 aaaaacgtct cccctccctc tttctgataa ataaaaaaca gtcgctc
//
LOCUS       DAXDXW010000029        22713 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXDXW010000029 DAXDXW010000000
VERSION     DAXDXW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22713
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.029"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(200..1039)
                     /locus_tag="ACFB64_000976"
     CDS             complement(200..1039)
                     /locus_tag="ACFB64_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFC1209352.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1324..1399
                     /locus_tag="ACFB64_000977"
     tRNA            1324..1399
                     /locus_tag="ACFB64_000977"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1357..1359,aa:Arg,seq:ccg)
     gene            complement(1464..2657)
                     /locus_tag="ACFB64_000978"
     CDS             complement(1464..2657)
                     /locus_tag="ACFB64_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC1209353.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGRMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDVYRSAVQHLLFGKGNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSERQWDEVLQIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMDLPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSSELYALWQNEVYMMRDRIRAMRQKLYGVLTARIP
                     DRDFTYFIKQRGMFGYTGLSVGQVRRLRDEFAVYLLDSGRMCVAGLNTSNITYVADAL
                     AEVLK"
     gene            complement(2677..3765)
                     /gene="trmA"
                     /locus_tag="ACFB64_000979"
     CDS             complement(2677..3765)
                     /gene="trmA"
                     /locus_tag="ACFB64_000979"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC1209354.1"
                     /translation="MNDYTQQLQGKKDYLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNHW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMKAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLRVGDRDFHYRQIEGSFTQPNAAVCRKMLEWACRAAEGLGGDLLELYCGNGNFTL
                     PLSRYFRQVLATEISKTSVSAAQWNIEANWIGNIKIARLSAEEFTEAYTGKREFTRLK
                     ESGIVLTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLTETH
                     TVGRAALFDQFPFTHHIESGVLLKKKIL"
     gene            3912..5012
                     /gene="aroC"
                     /locus_tag="ACFB64_000980"
     CDS             3912..5012
                     /gene="aroC"
                     /locus_tag="ACFB64_000980"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC1209355.1"
                     /translation="MAGNTFGQIFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATAF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKKIRFEGSEHISQNPFFAANQSQIAELEHYMDGVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDIHGNPVELATRGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAADTPDISRSDK"
     gene            5103..5528
                     /locus_tag="ACFB64_000981"
     CDS             5103..5528
                     /locus_tag="ACFB64_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC1209356.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5602..7587)
                     /gene="parE"
                     /locus_tag="ACFB64_000982"
     CDS             complement(5602..7587)
                     /gene="parE"
                     /locus_tag="ACFB64_000982"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC1209357.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATEIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVSVKRDGKVHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTSLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARIRSVKKIEKKKGSGVAILPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHAINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAQLDI"
     gene            complement(7654..8202)
                     /locus_tag="ACFB64_000983"
     CDS             complement(7654..8202)
                     /locus_tag="ACFB64_000983"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC1209358.1"
                     /translation="MNLTEGDTVLDREGYRPNVGIILINERNEVFWGKRVREHSWQFP
                     QGGIKPGESPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRG
                     QKQIWYLLRLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELS
                     SRFLRGMESYEDFAARQPSGNR"
     gene            8344..9639
                     /gene="serS"
                     /locus_tag="ACFB64_000984"
     CDS             8344..9639
                     /gene="serS"
                     /locus_tag="ACFB64_000984"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC1209359.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYEFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPPGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYGALEEMVGHAENILKALELPYRVITLCTGDMG
                     FSAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVTKLEVK"
     gene            complement(9712..10710)
                     /locus_tag="ACFB64_000985"
     CDS             complement(9712..10710)
                     /locus_tag="ACFB64_000985"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC1209360.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAACIFVNDDASRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEVEKLGGRYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKCRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10835..11911)
                     /gene="prfA"
                     /locus_tag="ACFB64_000986"
     CDS             complement(10835..11911)
                     /gene="prfA"
                     /locus_tag="ACFB64_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC1209361.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIDELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGGEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRTDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREVQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(12001..12771)
                     /locus_tag="ACFB64_000987"
     CDS             complement(12001..12771)
                     /locus_tag="ACFB64_000987"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC1209362.1"
                     /translation="MRLTDTHCHLADSVLRGNLAQVLAEARQAGVWRFIVPATCPQDW
                     QDVAELSEMPSEHGQIRIALGIHPWFSDGIAEQDFSGLEAVLVRYPRAWVGEIGLDFY
                     DKTQTPPQRERQIQVFSRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFTQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLENTV
                     NTPANILRIAEIAAEIRGTGAAEIAAITERNADSLLRP"
     gene            complement(12774..13766)
                     /locus_tag="ACFB64_000988"
     CDS             complement(12774..13766)
                     /locus_tag="ACFB64_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC1209363.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSAGLRPDTALVGQALAPFSDGMDFD
                     WHVCEPLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANMFALALQGL
                     DKPIVLTGSQWPYNAEGSDAPRNLVTAVAAFPLGLKQTLIAFDGKLYPAVGSSKISTE
                     TAAGFGNAHFGALAEWDETRGWHNVRIPNQDAADVSDGLEIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTSAGEGFIRAVQDFTQQGKLLLNISQVPQGC
                     AAAVYAQGDALRRAGVVNGGKCNLETATALMTLAVSEGWGADGVKHELQRLGLV"
     gene            complement(13833..14516)
                     /locus_tag="ACFB64_000989"
     CDS             complement(13833..14516)
                     /locus_tag="ACFB64_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC1209364.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGVMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14705..16039)
                     /gene="glmM"
                     /locus_tag="ACFB64_000990"
     CDS             complement(14705..16039)
                     /gene="glmM"
                     /locus_tag="ACFB64_000990"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC1209365.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNTYSDNGIKFFAEGGVKLSDEVELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPGHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGNEPNGYNINEK
                     CGATHTKTLQAAVLQNEADYGIALDGDGDRLMMVDKNRQVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQILNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIAAAIGGI"
     gene            complement(16169..17023)
                     /gene="folP"
                     /locus_tag="ACFB64_000991"
     CDS             complement(16169..17023)
                     /gene="folP"
                     /locus_tag="ACFB64_000991"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC1209366.1"
                     /translation="MARHVWRAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPGADFVPPEEEEWARVEPVLAEAAGWGVPVSLDTR
                     RTVVMEKALALGGIDIINDVAALTDEGAVELLARQADTGICLMHMRGLPETMQDNPKY
                     QDVVGEVARYLKTRSETCVAAGIAPQRITLDPGFGFGKNLQHNIALMRHLPELMAETG
                     LPLLIGVSRKSMIGELTGEADAAARVHGSVAAALASVARGAQIVRVHDVKATADALKV
                     WEALGVNR"
     gene            complement(17163..18056)
                     /locus_tag="ACFB64_000992"
     CDS             complement(17163..18056)
                     /locus_tag="ACFB64_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFC1209367.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWSNADAVFLDPFFTSANLYDTLSADEGRGRCRRLAEQAASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLGHTKGHPYRLDVESIVKVRQISHLPVIANITGLWSRDMPQEILY
                     GLAKAAGACGIVGTCFEKAGG"
     gene            18147..20249
                     /locus_tag="ACFB64_000993"
     CDS             18147..20249
                     /locus_tag="ACFB64_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC1209368.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTMLLLAAIGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIRRRLLPRPTVILKNLTITEPDGGRVAVSVKETKIGLSWK
                     NLWSDRIQVEKWVVSGADLALTRDRNGAWNIQDLFDGAKHSASVNRIIVENSTVRLNF
                     LQQQLILKEISLNLQSPDSSGQQFESSGILVWRKLSVPWKSRGLFLSDGIGTPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPALALK
                     NNSIKTGTVNGTFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRLQTNF
                     SLGSPLVWSRDNGLDAPRLHISTLQDTVDRLPQPRFISRLDGSLSIPNLQNWNAELNG
                     TFDRQPVAAKFKYTREGAPRLEAAAALQKLNLAPYLDEFRQQNGKIFPDILGRLSGNV
                     EAHLKIGSIQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTASGENRKQLIRSLQGSLSLNISN
                     GAWHGIDMDSILKNGLSGKISGSTPFYRFTLNSEISDGISRHIDTELFSDSLYVTSNG
                     YTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRKEKQK
                     ILEDTLLEQWQWLKPKEP"
     gene            complement(20313..21791)
                     /gene="ubiD"
                     /locus_tag="ACFB64_000994"
     CDS             complement(20313..21791)
                     /gene="ubiD"
                     /locus_tag="ACFB64_000994"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC1209369.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLVMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPSTILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGSDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQGHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFSEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMTGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATSKWPGETDREWGRVIKKDPAVTVKIDGIWGKLGL"
     gene            complement(21811..22326)
                     /locus_tag="ACFB64_000995"
     CDS             complement(21811..22326)
                     /locus_tag="ACFB64_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC1209370.1"
                     /translation="MHTCTSCGEKHEDMPATGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYSGQTDRFIRAALPIPIIGHQETLEYGVWVSVSEKSFNDYQSRFYDNPENAVYF
                     GMICNRLPPYETDTFGLHCNVVTQSDNQRPLLQLHQSGGHPPVRDFYHGMGYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22333..>22713)
                     /locus_tag="ACFB64_000996"
     CDS             complement(22333..>22713)
                     /locus_tag="ACFB64_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209371.1"
                     /translation="NLIHYITLFFAAVLVFGRRDSMYWYPVLVNLMMLAVFGSSLFAG
                     QTVIEKLARLQQPDLPEKAVRYTRRVTQVWCVFFIANGTLAALLARLGRYDWWAVYTG
                     VIAYVLMGMLFAGEWLYRKLVLKV"
BASE COUNT         5041 a         6554 c         6020 g         5098 t
ORIGIN      
        1 caaatgggtt aaaccagtat ccatacatca gcttttttat catcctactt tttattcatc
       61 cgatcgggca gacagatttc aaagatgaaa agcctattct attcacaccc atttgatgtc
      121 atttccacac ggacgaacaa ataaattccc ataaaaaaac ggagcagata cctgccccgt
      181 tttttaattt aatccgaatt tagaattttg caccggattg gtttgccata tagtcaacag
      241 ccgctttgac ttcgtcatcg ctcaaacctg cattgccgcc tttggccggc atcgcgttaa
      301 agccttcaag ggcatgtttg tgcaaggttt ctttgccttt tttgatacgc ggtgcccaat
      361 cgtctttttt gcctatgccg ggaataccgg gaatcgaacc gccgtggcac acctgacagg
      421 ttgcttcgaa gactttttta ccgtcaacgc cgaccgcagg ggctgccgca cccttgtctt
      481 ctgccttcgc ttctgccgga gctgcactat cggcaggagc agaagctgtt cctgaagcgg
      541 cattgtcggc aggcgcagcc tcatcaggat tcgggaaaga accgccgctt ttgttcgcca
      601 tgtaggtaat cgcccgtttg agttcctgat cggtcaggtc tgccgcaccg cctttggcag
      661 gcatggcgtt aaagccgttc agcgcgtgtt ggaacaaggt atcgaagcct tgcgcgatac
      721 gcggcgccca gtcgccgttg tgttccagtt tcggagcgtt cggcacattg ctgtccgccg
      781 cgtggcattg gatacagatt ttgccgaaaa tctgttcgcc ttggcgttcg ccgacgggga
      841 tgccgtcacc catcgtcaat tgtccgacag gctggatgcg ggtctgcgtt gccgcttccg
      901 tagtggcatc gacatcgccg aacgatccac tgcccgccag cttaatcagg aaataaagga
      961 ctgcaataac aataacgata ccgctcacaa gggtaaacag tgcagagcct tgggctttgt
     1021 tgtcgcggag ttgtttcatt tggtaggcct cgccgtcagg ttaggttgtg ctgtagatta
     1081 tagtttggcg tgttaaacac agttaacaat attttgctgg attatactga attcacaggg
     1141 tctttccaat cgctatcatt gaaaatatga aaaaatttgc caatggtatc tgtacaaaac
     1201 aaataatccg ttgaaaatac cggattatct ccaagaaaac tccccttgtg ccgccatacg
     1261 ccgcctgccg gcgcaagata acctttgcca atttgcagaa tttacgttaa ccttacgttt
     1321 tccgcaccca tagctcagtt ggaagagtgt cagtttccga agctggaggt cacaggttcg
     1381 atccctgttg ggtgcgccaa ttataaagag gccgtctgaa agataaatat ttttcagacg
     1441 gccttttgac ttacttcaaa ttcttatttc aacacttcgg caagcgcatc ggcgacatag
     1501 gtgatatttg atgtattcag cccggcgacg cacatcctgc cggaatccag caggtaaacg
     1561 gcaaattcgt cgcgcaacct gcggacttgc cccacgctca accccgtgta gccgaacatt
     1621 ccgcgctgtt tgatgaaata agtgaaatcg cggtcgggga ttcgggcggt caatacgccg
     1681 taaagtttct gccgcatcgc acggatgcgg tcgcgcatca tataaacttc gttttgccac
     1741 aaggcgtaaa gttcggagct gttcatcacg tcggcggcga tatacgcgcc gtgcgcgggc
     1801 gggctggagt agatgcggcg gacggtgaat ttgagttgtc cgaacaccaa atccgcttct
     1861 tctttattgg ggcaaaccac gcttaagccg ccgacgcgtt cgccgtagag cgacaggttt
     1921 tttgaaaacg aattgctgac aaacagcggc aaatccattt ccaccgcttt gcggacggcg
     1981 taggcatcgc tgtccaaatc gccgccgaag ccttggtagg cgatgtccat aaacggaatc
     2041 agtttgcgcg ttttgatgat ctgcaacact tcgtcccatt gccgttccga catatccacg
     2101 ccggtcgggt tgtggcagca gggatggagg atgaggacgc tgttttcggg cagggtgttg
     2161 aaaaacgcgg tcatttcgtc gaatttcacg ccgacagtgg cagggtcgta atatgggtaa
     2221 gtgccgacct cgaaacctgc gccttcaaaa atgccgcgat ggttgtccca agtcggatcg
     2281 ctgacgtagg cgcgcgcttc gggaaaccag cggtgcagga aatccgcccc gactttgagc
     2341 gcgcccgagc cgcccagagt ctgtacggta acgatgcgtc cctgcgcgag cgcggggttg
     2401 cctttgccga acaacaaatg ctgcaccgcg ctgcggtaaa cgtccaaacc ttccatcggc
     2461 aggtagggcg acggcgctgg cgcggcggca cgggcggttt cggcacggct tacggattcc
     2521 aatacgggca ttctgccttc gtcgtcgaaa taaatgccta tgctcaaatt gactttttcg
     2581 gggcgcgggt cgtttttgaa ggtttcgacc aaactcaaaa tcgggtcgcc gggatagtat
     2641 tcgatgtgtc ggtacatagt ccttaccttt tgcttttcaa aggattttct ttttcaacag
     2701 tacgccgctt tcgatatggt gcgtaaacgg gaattggtcg aacagggcgg cacgtccgac
     2761 cgtatgggtt tccgtcaagg tgtccaaatt ggcgcgcagc gtttcgggat tgcaggaaat
     2821 gtagatgatg ttgtcgaact gcgacaccag cttcaaagtt tcctcatcga tgccggcacg
     2881 cggcggatcg acgaaaatag tggaaaatgc gtaatccgtc aaaacaatac cgctctcctt
     2941 aaggcgtgta aactcacgtt ttccggtata ggcttcggta aactcctcag cagacaggcg
     3001 ggcgattttg atgttgccta tccagtttgc ctcaatattc cattgcgccg cactgacgga
     3061 ggttttggag atttcggttg ccaaaacctg tcggaaatat cgggacagcg gcagggtgaa
     3121 attgccgttg ccgcaataca gttcgagcag gtcgccgccc aagccttccg ccgcgcggca
     3181 cgcccattcg agcattttcc ggcatacagc ggcattcggc tgggtaaagc tgccttcgat
     3241 ttggcggtag tggaaatcgc ggtcgccgac gcgcagggtt tccgtcacat agtcctgttt
     3301 taagactatt ttctgccccc tgctccgccc gataacggaa atatccaact gttgctgtaa
     3361 cgcttgcgcc gccttcatcc actcatcatc aagccttttg tggtaaatca tggtaaccag
     3421 catttccccg ctgagcgtgg acagaaattc gacggcatac caatggtttc tcagttcggc
     3481 agattgggcg gcgacggcaa tcaattcggg catcaggcag ttgactgcct ctgaagcagc
     3541 atcgaaacgg tcgcaacgga tcaggcttgc cccgctagcc ttctgccctt tttcaaacat
     3601 ggcgtaaaac atttcaccgc cttcgtgcca aatgcggaat tcggcgcgca tgcggtagtg
     3661 tttgtccgga gattcgtaca cttcccactc aggaacatcc aaacctgcaa aaagggtttt
     3721 aaggtagtct tttttacctt gaagctgctg cgtgtagtca ttcatatcgc tatcctgaaa
     3781 aatcagaccg gattataagc cgattttcct cgccgcacgc aaactctttg actgccgccc
     3841 cttcaatgac ggacgggctt ttgtgctaaa atccgccatc tttccacact ataccgataa
     3901 agggaaaaat catggcaggc aacactttcg gacaaatctt caccgtgacc accttcggcg
     3961 aaagccacgg cgcgggtttg ggctgcatca tcgacggctg cccgcccggc ttggaattaa
     4021 gcgaagcgga tatccaattt gacctcgacc ggcgcaaacc cggcaccagc cgccacgtta
     4081 cccaacgccg cgaagccgac caagtcgaaa tcctctccgg cgtattcgaa ggcaaaacca
     4141 ccggcacacc catcgccctc ctaatccgca acaccgacca gcgcagcaaa gactacggca
     4201 acatcgccac cgccttccgc cccggacacg ccgactatac ctattggcac aaatacggta
     4261 cgcgcgacta ccggggcggc ggcaggagtt ccgcccgcga aaccgcagcc cgcgttgccg
     4321 ccggagccgt tgccaaaaaa tggctgaaag aaaaattcgg cacggaaatc accgcctacg
     4381 ttacccaagt cggcgaaaaa aaaatccggt ttgaaggcag cgaacacatt tcccaaaatc
     4441 ctttttttgc cgccaaccaa agccaaatcg ccgaactgga acactatatg gacggtgtgc
     4501 gcaaatcctt ggattccgtc ggcgcgaaac tgcatatcga agccgccaac gtccccgtcg
     4561 gtttgggcga acccgttttc gaccgcctcg atgccgaaat cgcctacgcg atgatgggca
     4621 tcaacgccgt caaaggcgtg gaaatcggcg caggttttga cagcgtaacg caacgcggca
     4681 gcgaacacgg agacgaactg accccgcaag gcttcctgtc caaccattcg ggcggcatcc
     4741 tcggcggcat cagcaccggg caagacatct gcgtcaatat cgccatcaaa cccaccagct
     4801 ccatcgccac accgcgccga agcatcgaca ttcacggcaa ccccgtcgaa ctcgccaccc
     4861 gcggcaggca cgatccctgc gtcggcttgc gcgccgcgcc gattgccgaa gctatgctcg
     4921 ccttggtttt aatcgaccac gccctgcgcc atcgtgcgca aaatgcggat gttgccgcgg
     4981 atacgcccga tatttcccgc tcggacaagt aaaaagattt agccaaaaca cgggctttat
     5041 actaaaatac cgaccattgc cgtgccgtct gaacgcacga ttcttagagg aatacaaccg
     5101 aaatgacaca agaaaccgct ttgggcgcgg cactgaaatc cgccgtccaa actatgagca
     5161 aaaagaaaca gaccgaaatg atcgccgacc acatctacgg caaatacgat gtattcaaac
     5221 gcttcaaacc gttggcgctc ggcatcgatc aggatttgat tgccgctttg ccgcaatacg
     5281 attccgcact gattgcacgc gtcctcgcca accactgccg ccgtccgcgc tatctgaaag
     5341 ccttggcgcg cggcggcaaa cgtttcgatt tgaacaaccg tttcaaaggc gaagttaccc
     5401 ccgaagaaca ggcgattgcg caaaaccatc ctttcgtgca gcaggctttg caacagcagt
     5461 ccgcccaagc tgccgccgaa acgccgtctg ttgaagccga agcagccgaa tcttccgcag
     5521 cagaataaat ccccaaacga aatgccgtct gaaaaccgat ttggtttcag acggcatttt
     5581 ttcgtatgcg gcaatcacgg ctcaaatatc gagttgcgcc gtatcgcctt cgcgttccat
     5641 ccaagcgcgg cgggcggcgg cttcgccttt gcccatcagt ttgacgaaga tgtcgcgcgt
     5701 ctcgtcatcc gcgccttcgg ggatttgcac ctgcaacagg cggcgggtgt cggggtgcat
     5761 ggtggtgtct ttgagctggt cggggttcat ctcgcccaag cctttgaaac ggctgatgga
     5821 ataggcggtt tctttaacgc cttctttttg cagccgctct aaaatgccgt caagttcgtt
     5881 ttggtcgagg gcgtagaatt tgcgggcggg tttgcttttg ccttgtgcgt tgacatcgac
     5941 gcggaacagc ggcggctggg cgacgtagat gtgtccgtcg gcgaccagtt tggggaagtg
     6001 gcggtagaac agggtcaaca gcaaaacctg aatatgcgaa ccgtccacgt cggcatcgga
     6061 caagatggct attttgccgt agcgcaggcc gcttaagtcg ggatggtcgt tgatggcgtg
     6121 cggatcgaca ccgatggcga cggaaatgtc gtggatttcg gcgttgccga aaagctggtc
     6181 ggggtggact tcaaagctat tgagcacttt gccgcgcagg ggcaggatgg cttgtgtggc
     6241 tttgtcgcgg gcgagtttgg cggagccgcc ggcggaatcg ccttcgacga ggaagagttc
     6301 gttttcgcgg atgtcttcgc tttcacagtc ggtcagctta ccgggcagga tggcgacgcc
     6361 gctgcctttt ttcttttcga tttttttaac cgaacgtatc cgtgcctgtg cctgacggat
     6421 ggcgagttcg gcgatttttt tgccgaagtc cacgttttgg ttcagccaca attccaaagg
     6481 atcgcccgat acggcggcga caagtttcag cgcgtcgcgg ttggtcagct tgtctttggt
     6541 ctgaccttgg aattgcgggt cgaggacgcg ggcggagagg acgaaggcgg tttttccgaa
     6601 cacgtcgtcg ctttgcactt taacgccgcg cggtaagagg ttgtgcaggt tgatgaaatt
     6661 gttgacggcg ttgaacacgg cttgtttcaa gcctgcttcg tgcgttccgc ccagcgaggt
     6721 cgggataagg ttgacgtagc tttcgttggc gcacgagcct tcttccagcc aagtcaaagc
     6781 aaacgcggca ccttcgccaa tgctgaaatc gccgttgtgg ccgtctgaaa tgtagttttc
     6841 gcaggagaac agcggcacgg cttcctgtgc gtcggcaatc aggtcggtca gatagctttt
     6901 cagaccgtcg gggtaatgcc aggtttgggt gtgcgcttcg tcttcgcctt tgaccggacg
     6961 ggtcagggaa acgcgcacgc ccggcagcag cacggctttg gcgcgcaata gacgctcgag
     7021 ttcgggaatg ctgtaattcg ggctttcaaa atatttgccg tccggccaca cgcgcacttc
     7081 ggtgccgctg tctttgacgg cgcatttgcc cacttgtgcc aacggttcga ccacatcgcc
     7141 gccggcaaac acgatgcggt ggactttgcc gtctcgtttg acactgactt caagtcgggt
     7201 ggaaagcgcg ttggtaacgg atacgcccac accgtgcagg ccgcctgaaa aggcatacgc
     7261 actgccgccg tcttttttgt tgaacttgcc gcccgcgtgc aggcgggtaa acacgagttc
     7321 gactacgggt acgccttctt cagggtgcag gccgacggga atgccgcgcc cgttgtcgcg
     7381 cacggaaagc gaaccgtctt catgaatttg cacgtcgatt tcagtggcga aaccgcccaa
     7441 cgcctcgtcc gccgcgttgt cgatgacttc ttggcagata tgtgtcgggc tgtcggtgcg
     7501 ggtgtacatg ccgggacgtt ctttgaccgg ctccaagcct ttgaggacgg tgatgctgga
     7561 ttcgctgtat tggttgtttt tagccatagg aataatctga aagtaagaaa aacaacgctt
     7621 tcagacggcc tgaaagcgtt gcgttccgtt gttttaccgg ttgccggaag gttggcgggc
     7681 ggcaaagtct tcataacttt ccataccgcg caggaagcgg gaagagagtt ctttcaacgc
     7741 ccccaaataa acgtcgcgtt tgaaatcaat cacttcgtca acgggcgccc aatattgatg
     7801 ccaacgccag ccgtcaaatt cggggtggcg ggtggcgcgc aggttgacat cgcaatcccg
     7861 gccggtcagg cgcaggagat accaaatctg cttctgcccg cgataagagc cgcgccattc
     7921 gcggcgcacc cagttgttcg gcacgtcgta acgcagccag tcgcgcgtcc gcccgacgat
     7981 tttgacgtgt tgcggcagaa gtccgacttc ttcgtaaagt tcgcggtaca tcgcggtttc
     8041 ggggctttcg ccgggcttga tgccgccttg aggaaactgc caagaatgtt cgcgcacgcg
     8101 cttgccccaa aagacttcgt tacgttcgtt aattaggata ataccgacat tggggcgata
     8161 gccttccctg tccaacacgg tgtcgccctc cgttaaattc aatcttggga ttttcccaca
     8221 aatcggacgg ttttgacaaa tcggacggta tggttcaagc atgtcgaaac acggacggat
     8281 tcgggaaaat atcttaaatt tggtttacaa taatacgttt caaattaatt ccggaatcag
     8341 actatgttag acatccaatt gctccgcagc aacaccgccg ccgttgccga acggcttgca
     8401 cggcgcggtt atgaattcga taccgcgcgt tttgacgcac tggaagagcg acgcaagtcc
     8461 gttcaggtga aaaccgaaga actacaagcc tcgcgcaaca gcatttccaa acaaatcggc
     8521 gcactgaaag gtcagggcaa acatgaagag gcgcaggcgg ccatggatca agtcgcccaa
     8581 atcaaaaccg atttggaaca ggctgccgcc gatttggatg ccgttcaaaa agaattggac
     8641 gcatggttgt tgagcatacc taacctgccg cacgaaagcg tacctcccgg taaagacgaa
     8701 accgaaaacg tcgaagtccg caaagtcggt acgccgcgcg aatttgattt tgaaatcaaa
     8761 gatcatgtcg atttgggcga gcctttgggc ttggatttcg aaggcggcgc gaaactctcc
     8821 ggcgcacgat ttaccgtgat gcgcggacaa atcgcccgtc tgcaccgcgc cttggcgcaa
     8881 ttcatgctcg atacgcacac gctgcaacac ggctacaccg agcattacac gccttatatc
     8941 gttgacgata cgacgctgca aggcacaggt cagctgccca aatttgcaga agatttgttc
     9001 cacgttaccc gcggcggcga tgaaaccaaa accacgcaat atctgattcc gacagccgaa
     9061 gttaccctga ccaataccgt tgccggcagc attatcccgt ccgaacaact gccgctgaag
     9121 ctgaccgcgc attcgccctg tttccgcagt gaagcgggat catacggcaa agacacgcgc
     9181 ggcctaatcc gccagcacca gttcgacaaa gtagaaatgg tacaaatcgt tcatcccgaa
     9241 aaatcatacg gcgcgctgga agaaatggtc ggccatgccg aaaacatcct gaaggctttg
     9301 gaactgccct accgcgtgat taccctgtgt accggcgaca tgggtttcag cgcggccaaa
     9361 acgtatgact tggaagtttg ggttcccgca caaaatacct accgcgaaat ctcaagctgc
     9421 tccaactgcg aagatttcca agcacgccgc atgaaggcgc gtttcaaaga cgaaaacggc
     9481 aaaaaccgct tggtacatac cttaaacggc tccggtttgg cagtcggtcg cactttggtc
     9541 gcggttttgg aaaaccatca aaacgccgac ggcagcatca atatccccgc cgcactgcaa
     9601 ccgtatatgg gcggtgttac caagttggaa gtgaaataag ccttaaccct gaacataaat
     9661 gccgtctgaa acctgtttca gacggcattt cctttaaact cttaaaacac atcaaccgtc
     9721 ggcacgaacc gcattgccgc aatcgccggt ctgtccgacc tcgcggatat tggacagcgt
     9781 aacttccgaa atattaccca acgcctcttc cgtcaaaaat gcctgatgtc cggtaaacag
     9841 tacgttatga caagacgaca ggcggcggaa cacgtcgtcg gtaatcacat cgttggattt
     9901 gtcttcaaaa aacagctcgc gctcgttctc gtacacatcc atgcccaatg caccgatttt
     9961 ccggcatttc aacgcctcga tggcggcggc gctgtcaatc agcccgcccc ggctggtgtt
    10021 gataatcatc acgccgtctt tcattttgtc gaacgccgct tcgttcagca tatagtggtt
    10081 ttccggcgtg gcgggacaat gcagcgtgat gatgtccgat cgggcataca gctcgtccaa
    10141 atccacatac ctgccgccga gtttttccac ttcggggttg caaaacggat cgtaagccag
    10201 caggttcata ccgaaaccct ttaaaatccg catcgttgcg ataccgattt tccccgtgcc
    10261 gataacgccc gccgttttgc cgtacatatt gaagccggtc agaccttcca gcgaaaaatt
    10321 cgcatcgcgg gtacgctggt aggctttgtg gatacggcgg ttcaaagtca gcatcagacc
    10381 gaccgtatgt tccgcaaccg attcgggcga ataggcaggc acgcgcacga ctttcaagcc
    10441 caactcttca gccgccttca aatccacatt attgaagccg gcacaacgca acgccacagt
    10501 tttcacgcca atttgcgcca atttttccaa cacgggccgg ctggcgtcgt cgtttacaaa
    10561 aatacaggcc gcttccgcgc cttccgccat tttcgccgtt ttcgcatcca gcatgaaatc
    10621 aaaaaactcc agctcgaagc cgaaatgccg gttggcgcgg gtaaaatgtt cgcggtcata
    10681 gcttttcgta ccgtaaatcg caatcttcat caatatgtcc agttgtcgtc tatggttgag
    10741 aaacggcata atacaccgaa ttcaaacaaa tcagtagaat atggcggatt aaaattgatt
    10801 tcatgcacgg catttccatt tcaaaacaca aaactcaatc gcccatcgcc gccagaagct
    10861 ccgcctgatg ttcggcaatc agggcatcgg tgatttctgc caaatcgccg tccatcacaa
    10921 aatccagctt gtgcagggtg aggttgatgc ggtggtctgt taccctgcct tggggatagt
    10981 tgtaggtgcg gatgcgttcg ctgcggtcgc cgctgccgat gagggatttg cgttcggcgg
    11041 cttctttggc ttggacttcg cgtttttgcg cgtcgttcag gcgggcggcg aggactttca
    11101 tcgcctgcgc tttgttggcg tgttggctgc ggccgtcttg gcattcgacc accatgcctg
    11161 tcggcaagtg ggtaatgcgg acggcggagt cggttttgtt aatgtgctga ccgcccgcgc
    11221 cggatgcgcg gaaggtatcg gtgcgcaggt cggcggggtt cagttcgatg tcttcgagct
    11281 cgtccgcttc gggcatgacg gcaacggtac aggcggaggt gtggatgcgg ccttggcttt
    11341 cggtggcggg gacgcgctgc acgcggtgtc cgcccgattc aaatttcaga cggctgtacg
    11401 cgccgagtcc gacaatacgg gcgatgactt ctttatagcc gcccaattcg ctttcgttgg
    11461 cggacacgat ttcaacctgc cagcggttgc gttcggcgta gcggctgtac atacgcagca
    11521 aatcgccggc aaacagcgcg gcttcgccgc cgcccgtgcc ggcgcgtatt tcgataaaga
    11581 tgtttttgtc gtcgtcggca tctttgggca gcagcagttt ttgcaattcg gtatcgagtt
    11641 cgtcgatttt ggctttcgcc gcttcgattt cttcggcggc aaagtctttc atttcggggt
    11701 cggacaacat ttcttcggca tccgccaagt cgctttgggc aagccgatag ttttggaata
    11761 cttcgacgac gggggtcagt tcggcgtgtt cgcgcgtgag cttgcggtag ttgtccatat
    11821 cggatgtggc ttcgggttgc cctaaaaggt gggtaacttc ttccaaccgg tcgccgagtt
    11881 gttgcagttt ttctaagata gacggcttca taattcttcc ataacaaacg ccgcctgaat
    11941 ttcagacggc atcaaaacgc ggtcattata atatattttc tgaatattca aaaagataat
    12001 ctagggacgg agcagactgt cggcattgcg ttccgttatc gcggcgattt ccgccgcgcc
    12061 ggtaccgcgt atttccgcag caatttcggc aatccgaaga atgttggcag gcgtattgac
    12121 ggtgttttcc aacatgaacg ggctatccgt ttccaatacg aaatccccgt cgttcaaggc
    12181 tttaagcgta tcgcgcactt tacgcgcgtt cggattgagc agcagcgaac cgatgccgat
    12241 tttgaacccc agttttgtca acacgcgcgc ttcttccgcg ctgcctgaga aagcgtgaac
    12301 gatgccgcct tgagtaaagc ctgtctgttt gacggcggcg gcgatgtcgg cggtggcttt
    12361 gagattatgg ataatcacgc ggcggcgcag ggtttgcgcg atggcaagct ggcggctaaa
    12421 aacttgaatt tgccgttcac gctgcggcgg cgtttgggtt ttgtcgtaaa aatccaagcc
    12481 gatttcgccg acccacgctc gggggtaacg caccaatacg gcttccaaac cggaaaaatc
    12541 ctgttccgca atgccgtctg aaaaccaagg atggatgccc agcgcaatac ggatttgacc
    12601 gtgttcggac ggcatttccg acagttccgc cacgtcctgc caatcctgcg gacaggtggc
    12661 gggaacgata aaccgccaca cgcccgcctg tcgcgcttcg gcaagcacct gcgccagatt
    12721 gccgcgcaaa acggaatcgg caagatggca atgtgtgtcg gtcagcctca tcttcacacc
    12781 agccccaacc gctgcaactc gtgtttcacc ccgtccgcgc cccatccttc ggaaacggca
    12841 agcgtcatca atgcggttgc ggtttccaaa ttgcatttgc cgccgttgac cacgcccgcc
    12901 cgacgcagcg cgtcgccctg tgcgtaaacg gcggcagcgc aaccctgcgg cacttggctg
    12961 atgttaagca acaatttgcc ttgctgcgta aagtcttgca cggcgcggat gaagccttcg
    13021 ccggcggagg tattgccgtg tccgtagctt tgcaggataa gggcttgggc gggaagctgt
    13081 ccgagcccgt cggcaagttc ttggacggca aagccgggga taagcgtgcg gacggcgatt
    13141 tttgcctgcg ggtcgggata acggatttcg aggccgtctg aaacatctgc cgcgtcctga
    13201 ttcgggatgc ggacattgtg ccagccccgg gtttcgtccc actcggcaag tgcgccgaaa
    13261 tgggcattgc cgaaaccggc ggcggtttcc gtgctgattt tgctgctgcc gacggcggga
    13321 tacagtttgc cgtcaaaggc aataagcgtc tgcttcaatc cgagtgggaa tgcggcaacg
    13381 gcagtcacca gattgcgcgg cgcgtcgctg ccctctgcgt tgtacggcca ttgcgaaccg
    13441 gtcaggacga tcggtttgtc caaaccttgc agggcgaggg cgaacatatt ggcggtatag
    13501 gcgagtgtgt ccgtgccgtg cagcaccaaa acaccgtcgt actgcggcag cgcggcggca
    13561 atgatgtcca gccaagcgca ccagttttgc agttggacgg cggaagaatc aatcagcggc
    13621 tcacaaacgt gccaatcgaa atccatgccg tctgaaaacg gcgcgagtgc ctgcccgacc
    13681 agcgcggtat cggggcgcaa gcccgcactg ctttgcgtca tgccgatggt gccgccggta
    13741 taaaggacaa aaattttctg tttcatggct cattctgaaa atgcaaagac cgcctgaaac
    13801 gcgaaatgcg tccggacggt cgaaaatccg atttactgct gcttctgtgc cgcttttttc
    13861 gctgcctttt ccgccttgcg tttggcgcgt ttttcgctca attgtgcgcg gtaaagctga
    13921 taatgtcggc gtttgcgcca ccagaaccac gccagcgccg ccgccagcac gcccaatgcg
    13981 atgaagatgc ccgattgcag gctgtgcatt ttcgccatca gccaatcgat gttgtgcgcg
    14041 ccgtactcgc ccaagtaaat ccaaacgggc acggaaatca gtgcggccag cccgtccata
    14101 atcagaaagc gcagatacga tactttgcgg ctgatgccgg cggtaacgaa aacggcagtc
    14161 cgcaaacccg gcaggaaacg ggcgacaaac agaacccagt tgccgtattt gtcgaatttt
    14221 tcctgaacct gcgcgtaacg tttcggcgtc atgatgcgcg caatcggttt gaacttgagg
    14281 attttctgcc cccagatgcg tccggcggca aacatcacgc cgtcgcccgc caacacgccg
    14341 agcataccga ccgcaaacat aatatgcgga ttggtataac ccatacccga aatcacgccg
    14401 cccgttacca aggtcaaatc ttcgggaatc ggcacgccga aaccgcagat gaccaaaacg
    14461 aaaaacacgg ccgcatagcc gtattcgaca aaaaaggctt ccaaaagggc aaacatagcg
    14521 gatattccat tgtcggagat aaaaagtcag aacaagccga aacattttct gcgcgaaacg
    14581 gcattctatc aaagattatg ccgtctgaaa gcggcagggg gggcagattc gcgccccgcc
    14641 ggacgttcag acggcattta aacaggaaaa ccgccgctgt tgaaacggtt ttgcctgatt
    14701 ttgcctaaat gccgccgatg gcggcggcga tacgctccgc cccgtcgcgc gcccaatcgg
    14761 cctgacgcgc ctcgaccatc acgcgcacga cgggttcggt tcccgaagcg cgcaacacga
    14821 cacgcccttt gccttcgagt tctttttcca cttcggccaa cacgtctttc gaagcctcct
    14881 gccatttctg acccttttgg atgcgcacgt taatcatcgt ttgcggatac ggctgccagt
    14941 cggcgcaaac ggtggcgagg tcttggttca ggatttgcag cgccgccaag acttgcagcg
    15001 cggaaataat gccgtcgccg gtgttatgtt tgtccatgca caaaatatgg ccgctggctt
    15061 cgccgccgat gagccagccg cgttggttca gctgttccaa cacatagcgg tcgccgactt
    15121 tggcgcggca gaaatcgacg ccctgctctt tcagggcgat ttccatcgcc atattggtca
    15181 tgaccgttcc gaccacgccg ccgatgttga taccttctcg ggcgcgggct ttggcgatga
    15241 cgtaaatcag gctgtcgccg tcgtaaacct gcctgttttt atcgaccatc atcaggcggt
    15301 cgccatcgcc gtccaaggca ataccgtaat cggcttcgtt ttgcaacacg gcggcttgga
    15361 gggttttggt atgggtcgcg ccgcattttt cgttgatgtt gtagccgttg ggttcgttgc
    15421 cgatgctgac gacctgtgcg ccgagttcgt ggaacacttt gggtgcaacg ccgtatcctg
    15481 caccgttggc ggtatcgata accaacttca aaccccgaag gtcggaatgg ccggggaagg
    15541 tggatttgca aaactcgata tagcggtcgt ccgcaccgct gatgcggcgg gcgcgtccga
    15601 gacgtgcgga cggttgggtt ttcatttctt catcgatttt ggcttcgatt tccaactcga
    15661 cttcatcgga aagtttcacg ccgccttcgg caaagaattt gatgccgttg tcggaatagg
    15721 tgttgtgcga cgcggaaatc atcacgccgg cggacaggcg caatgcgcgg gtcagataag
    15781 ccacaccggg cgtgggcaac ggcccggtct gtaccacatt cacacccgcc gccgtaaagc
    15841 ccgctaccag cgcggcttcc agcatatagc ctgaaatgcg cgtgtctttg ccgatgagga
    15901 cggtcggttt ctgatcggta tcgtgctgca ccagcacctg ccccgccgca tagccgagtt
    15961 tcaatacaaa atcgggcgta atcggaaact gaccgacttc gccgcgcacg ccgtccgtgc
    16021 cgaaatattt ttttgccatc atttgctccg tgaatgtgaa ccgttgtccg agattataca
    16081 gtcagtttgc gccctgctgc ctgcaccgtt gatgccgtct gaagccgtcc cgcccttttc
    16141 agacggcatt cggcgcaaaa accgccgttt accggttcac gcccaacgct tcccacacct
    16201 tcaacgcgtc cgccgtcgcc ttcacatcgt gcacgcgcac gatttgcgct ccgcgtgcca
    16261 cggaagccaa agccgctgcc acgctgccgt gtacgcgcgc cgccgcgtcc gcctcaccgg
    16321 tcagctcacc tatcatgctt ttgcgcgaca caccgatcag cagcggcaaa ccggtttccg
    16381 ccatcaattc gggcaaatgc cgcatcagtg cgatattgtg ttgcaggttc ttgccgaagc
    16441 cgaaacccgg gtcgagcgta atccgttgcg gcgcgatgcc tgccgcgaca caggtttctg
    16501 accgtgtttt cagataacgt gccacttcgc cgaccacatc ctgatatttc ggattgtcct
    16561 gcatggtttc gggcaagccc cgcatatgca tcaggcaaat gcccgtgtcc gcctggcgcg
    16621 ccagcaattc gaccgcacct tcgtcggtca acgccgccac atcattgata atatcgatgc
    16681 cgccgagtgc caacgccttt tccataacca ccgtgcggcg cgtgtccaaa ctgacgggaa
    16741 caccccaccc cgccgcttcc gccaatacag gctcaacccg cgcccattct tcttcttcgg
    16801 gcggaacgaa atccgcaccc ggccgcgtcg attcgccgcc gatgtcgaga atgtccgcac
    16861 cctctttcaa aagccgttcg gcatgcgcca aagctgtttg ggcgttttgc gaatacgcgc
    16921 cgccgtcgga aaaagaatcg ggcgtgagat tcacgatgcc catgattttc ggtttgtcca
    16981 aaccgatttc aaaccgtcct gcccgccaaa cgtgtcgtgc catctgaact cctcccgaaa
    17041 taaaaaacag attatatgcc gtctgaaacc gtcttgtgcg cttcggacgg caccgctatt
    17101 cgggcggcag acggcatgtt gtcccaatgt ctgcgccgcc tttgaatctg ccggcatgcc
    17161 tgctatccgc ccgccttttc aaaacaggtt ccgacgattc cgcacgcgcc tgccgccttt
    17221 gccaagccgt acaggatttc ctgtggcata tcgcggctcc ataatcccgt aatattcgca
    17281 atcacgggca gatggctgat ttggcggact ttcacgatgg attcgacatc caaacggtag
    17341 ggatggcctt tggtatggcc caacacgccc gactcgccca gaatcaggtg gcggttgccg
    17401 cgcgagacga catattctgc ggcattcaac caatcttcgg cagaatggtg cttgtcttta
    17461 cacagaacca agggaatgtt caggcttccg gcttcattca atacggcaag atcggacatc
    17521 agcccgccgc ccaaatacag gatgtccgct cccgcattca aagccgcttc gacatggcgg
    17581 acgttgcgaa cgcgcaccaa tacgggtttc cctgcgccat gcgccgatgc ggcctgttcc
    17641 gccaaccgtc tgcaccgtcc ccgcccttca tccgcactta aagtgtcgta taagtttgcc
    17701 gaagtgaaaa acggatccag aaacactgca tccgcattgc tccatactga cggttctgca
    17761 gcgatacgga cggtttcccc gccgccgaaa gccacgcctt tggcggcaac gcggctgtct
    17821 tccgcccgat tttcccgact gacggttttc catgtgtcca aaatgcggac ggctttctcg
    17881 acctccggca gcgtctgtac ctccctgacg ctcaaaaccc tatcgtcgcc gattgcgccg
    17941 atgacggtac gctcgtcgcc gtgagaaatg tgttctcgca gacctctgct gcggatgaag
    18001 gcgacaacgc cggcaatgtc cgcttcggcg gcacgcctgc tcatgacaat aatcatattt
    18061 cctcctgaca caagagacgg cccgcccaaa ataggatttt tgcaagccgt gttatactgt
    18121 ggcgtgtttt acagattgtt cgggctatgg atttattatc ggttttccac aaataccgtc
    18181 tgaaatatgc ggtggcggtg ctgacgatgc tgcttttggc ggcaatcggg ctgcacgctt
    18241 ccgtatatcg caccttcacg cccgaaaaca tccgcagccg cctccaacaa agcattgccc
    18301 atacccaccg gaaaatctcg tttgatgcgg atatacggcg caggcttctg ccccgcccga
    18361 ccgtcatcct gaaaaacctg accattaccg aacccgacgg cggccgggtc gccgtttccg
    18421 tcaaagaaac caaaatcgga ttgagctgga aaaacctgtg gtcggatcgg atacaggttg
    18481 aaaaatgggt ggtttcgggt gcggatcttg ccctgacgcg cgacagaaac ggcgcttgga
    18541 acatccaaga cctgttcgac ggcgcgaaac actccgcctc agtcaaccgc attatcgtcg
    18601 aaaacagcac cgtccgcctc aatttcctgc agcaacagct tatcctgaag gaaatcagcc
    18661 tcaacctgca atcccccgat tcgtcggggc agcagtttga aagttcgggc atactggttt
    18721 ggagaaagct gtccgtcccg tggaaaagca gggggctgtt cctttcagac ggcatcggca
    18781 cgcccgaaat ctcaccgttc cattttgaag cttccacttc gctggacgga cacggcatca
    18841 ccatttccac caccggcagc ccttctgtcc gcttcaacgc cggcggagcg gatgccgccg
    18901 gcctcggcct gcgtgcagac acttccttcc gcaacctcca cctgaccgcg caaatccccg
    18961 cactggcact caaaaacaac agcatcaaaa ccggcacggt caacggcacg tttaccgccg
    19021 gcggcgaata tgcccgatgg gacggttcgt tcaaactcga caaagccaac ctgcactccg
    19081 gcatcgccaa catcggcaac gccgaaatct ccggcagctt caaaacaccg cgccttcaaa
    19141 ccaatttctc cctcggctcg ccgttggttt ggagtcggga caacgggctg gacgccccgc
    19201 gcctgcacat atcgaccctt caggataccg tcgaccgcct gccgcaaccc cgtttcatca
    19261 gccggctcga cggttcgctg tccataccga atctgcaaaa ttggaatgcc gaattaaacg
    19321 gcacattcga ccgccaaccc gttgccgcaa aattcaaata tacgcgggaa ggcgcaccgc
    19381 gcctggaagc cgccgccgcg ctgcaaaaat taaacctcgc cccctatctt gacgaatttc
    19441 ggcaacaaaa cggcaaaata ttccccgaca tcctcggcag gctgtccggc aacgtcgagg
    19501 cacacctcaa aatcggcagc atccaactcc ccggcttgca actggacgat atggaaacct
    19561 acctccacgc cgacaaagac catatcgcgc tcagccgttt caagtcaggg ctttacggcg
    19621 gccataccga aggcggcatc agcatcgcca acacccgtcc cgccacttac cgcctgcaac
    19681 agaatgcaag caacatccaa atccaaccgc tgctgcaaga cctgttcggc ttccacagct
    19741 tcagcggcaa cggcgatgcg gtcatcgacc tgaccgcaag cggcgaaaac cgcaaacagc
    19801 ttatccgctc gctgcaaggc agcctgtcgc tgaatatttc caacggcgcg tggcacggca
    19861 tcgatatgga cagcatttta aaaaacggcc tttccggaaa aatctcgggc agcacaccct
    19921 tctaccgatt cacgctcaac agcgaaattt cagacggcat cagccgccac atcgataccg
    19981 aactcttctc cgacagcctc tatgttacca gcaacggcta taccaatctg gatacgcagg
    20041 aattgtctga agatgtcctt atccgcaacg ccgtccatcc gaaaaacaaa ccgattcccc
    20101 tgaaaatcac cggtacggtg gacaagccgt ccattaccgt cgattacggc aggctgaccg
    20161 gcggcatcaa ttcgcgcaaa gagaaacaga aaatcctcga agacaccctg ctggaacaat
    20221 ggcagtggct caaacctaaa gaaccgtaaa taccctgcgt acaaaaatgc cgtctgaaac
    20281 acattaatct tcagacggca tgaccgtaaa acctacaacc ccaatttccc ccaaatccca
    20341 tcaattttaa ccgtaaccgc agggtctttt ttgattaccc gtccccattc gcggtcggtt
    20401 tctcccggcc atttgctggt cgcatccaaa cccattttgc cgccgagtcc gctgacgggg
    20461 ctggcgaagt cgaggtagtc gatgggcgtg ttttccacca aaacggtgtc gcggacgggg
    20521 tccatgcgcg tggttaccgc ccagatgact tctttccagt cgcgcacgtt tacatcgtca
    20581 tccaccacga tgatgaattt ggtgtacata aactggcgca ggaacgacca gcagcccgtc
    20641 atcacgcgct tggcgtgtcc ggcgtactgt tttttcatgc tgaccaccgc catgcggtag
    20701 gaacagcctt cgggcggcag gtagaaatcg gtgatttcgg agaactgctt ttgcaaaagc
    20761 ggtacgaaca cttcgttcaa cgccacgccc aaaacggcag gttcgtcggg cggtttgcct
    20821 gtgtaggtag agtggtaaat cgggttttcg cgcatggtga tgcgttcgac cgtaaacaca
    20881 gggaaatggc cctgctcgtt gtaatagccc gtgtggtcgc cgtatgggcc ttccaacgcg
    20941 gtttcgtttg gatgaatcac gccttccaat acaatttcag cacgggcagg cacttgcaaa
    21001 tcgctgccga tacatttcac cagctccgtc cgcgaaccgc gcagcagtcc ggcaaactgg
    21061 tattcgctca aagtatcggg aacgggcgtt accgcgccca aaatggtgga ggggtcgcaa
    21121 ccgagtacga cggcgacggg atacggcgta tcgggattga gtttgcggaa ttcctgataa
    21181 tccaacgcgc cgccgcgatg cgacagccag cgcataacca gcttgttttt gccgatgagt
    21241 tgttggcggt aaatgccgag attttggcgt tttttgtgcg gcccgcgcgt gacggtcaag
    21301 ccccacgtta ccagcggcgc aacgtcttcc ggccagcaat gttgaatcgg aagctgatac
    21361 aaatcaacgt cttctccttc ccacacgatt tcctgacacg gcgcgttttt caccacgttc
    21421 ggcgccatgc tccaaatatc tttcaacagc ggcagtttgg aaaacgcgtc tttgatgcct
    21481 ttgggcggtt cgggttcttt caaatacgcc agcgtctgcc cgatttcgcg cagcttggac
    21541 acgctgtccg cgcccatgcc catcgccaca cgttcggggg tgccgaacag gttcgccaac
    21601 acgggataat catagcgcgt accgtcgggc ttaaccgggt tttcaaacaa caacgccggc
    21661 ccttcggcgc gcaacacgcg gtcggcaatt tcggtcattt ccaaatgcgg ggaaacgggg
    21721 tgggcgacgc gcttgagctt gccctgctgc tcgagcatag cgatgaagtc gcgcaggtct
    21781 ttgtatttca tgtttatcct ttttgtcctt ttatcctgaa caatccgatt cggataccgc
    21841 ccctatcctt gcctgcgctt cggcatatcc catgccgtga taaaagtcgc gtaccggcgg
    21901 atgtccgccg ctttgatgaa gttgcagcaa agggcgttgg ttgtcggact gggtaacgac
    21961 attgcagtgc agaccgaagg tgtcggtttc ataagggggc agccgattgc aaatcatacc
    22021 gaaataaacg gcgttttcag ggttgtcgta aaagcggctt tgatagtcgt taaaactctt
    22081 ttcgctgacg gacacccaca cgccgtattc cagcgtttcc tgatgcccga taatcggaat
    22141 aggcagcgcc gcgcggataa agcggtcggt ttggccggaa tagcggatga tgcagaaatc
    22201 agaatcgcat tccgcttgat aagcaatgcg ttcttcttca ctgagttgat tatagggata
    22261 gggggcggta aagccggttg cgggcatatc ttcatgtttt tcgccgcagg aagtgcaagt
    22321 gtgcataagg tttcagactt tcaacacaag tttgcggtaa agccattcgc cggcaaacag
    22381 catccccatc aatacatagg caatcacgcc tgtataaacc gcccaccaat catatcgccc
    22441 caaccgggcc aacaaagcgg caagcgtccc gttggcaata aagaatacgc accaaacctg
    22501 cgttacccgg cgggtatagc gcacggcttt ttcaggcagg tcgggctgtt gcagccgcgc
    22561 aagtttttca atcaccgtct gcccggcaaa caggctgctg ccgaacaccg ccaacatcat
    22621 caaattaacg aggacaggat accaatacat tgaatcacgc cgcccgaaca ccaatactgc
    22681 ggcaaaaaac agtgtaatat agtggattaa att
//
LOCUS       DAXDXW010000030        22278 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXDXW010000030 DAXDXW010000000
VERSION     DAXDXW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22278
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.031"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..120
                     /locus_tag="ACFB64_000997"
     CDS             <1..120
                     /locus_tag="ACFB64_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HFC1209372.1"
                     /translation="ILAVVPKKAEAISNKLFFTHHPKRQTEFAVGQIRQGRAE"
     gene            248..1543
                     /gene="hisS"
                     /locus_tag="ACFB64_000998"
     CDS             248..1543
                     /gene="hisS"
                     /locus_tag="ACFB64_000998"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC1209373.1"
                     /translation="MAQKIQSVKGMNDLLPVGQKDFKLTAVFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEAQLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENSRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELADGTVTLKDMNGAHGQQTVSATDLTDTLQQWKNA"
     gene            1544..2173
                     /locus_tag="ACFB64_000999"
     CDS             1544..2173
                     /locus_tag="ACFB64_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC1209374.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALMILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELSKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQEKSQEALKNYGQALEKMPQDSVGRELLQMKLDSLK"
     gene            2323..3780
                     /gene="der"
                     /locus_tag="ACFB64_001000"
     CDS             2323..3780
                     /gene="der"
                     /locus_tag="ACFB64_001000"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC1209375.1"
                     /translation="MKPTIALIGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYFVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGDRAVLAAEFYELALGEPHVISGAHGDGVYYLIEEIL
                     ENFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEKRVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDISRKLYFLDFAKFHFISALKERGI
                     DGLFESIQAAYNAAMIKMPTPKITRVLQTAVGRQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            3811..4710
                     /locus_tag="ACFB64_001001"
     CDS             3811..4710
                     /locus_tag="ACFB64_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC1209376.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTRLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            4762..5179
                     /locus_tag="ACFB64_001002"
                     /pseudo
     CDS             4762..5179
                     /locus_tag="ACFB64_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5246..5812
                     /gene="dcd"
                     /locus_tag="ACFB64_001003"
     CDS             5246..5812
                     /gene="dcd"
                     /locus_tag="ACFB64_001003"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC1209377.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGQRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEVCETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(5878..6582)
                     /locus_tag="ACFB64_001004"
     CDS             complement(5878..6582)
                     /locus_tag="ACFB64_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC1209378.1"
                     /translation="MLRSVLAASLLAVSFPAAAEALNYNIVEFSESAGIEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKAEGRDFDALNRFIADVQTDASLEDTDFSVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNFGQIGSHIAGDGAVRAKMLRAMPMAASVNMKGTDSAA
                     PGVEEISISINGMVQF"
     gene            6726..7921
                     /locus_tag="ACFB64_001005"
                     /pseudo
     CDS             6726..7921
                     /locus_tag="ACFB64_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            7947..8900
                     /locus_tag="ACFB64_001006"
     CDS             7947..8900
                     /locus_tag="ACFB64_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC1209379.1"
                     /translation="MTHTVHLHLEETDNPTLQRLCGSFDSNLDSLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDSIIRLAAVETQTEDAGHQEKNHEHAYYF
                     RTKRGSIGGRTPRQNGYIRALLDHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVAGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(9005..9328)
                     /locus_tag="ACFB64_001007"
     CDS             complement(9005..9328)
                     /locus_tag="ACFB64_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209380.1"
                     /translation="MKKCILGILTACAAMPAFADRISDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCGDEAIR
                     CRKFD"
     gene            complement(9501..10859)
                     /locus_tag="ACFB64_001008"
     CDS             complement(9501..10859)
                     /locus_tag="ACFB64_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC1209381.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGNAAERYQEDPVRILRAIRLSGKLGFELSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDIHPLLNALRVSDGIAGKM
                     TALALKNTDERLRADKSVSVGFVLAALMWPELDRHWKSNLQQGLKPTPALSDAINTMR
                     ETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAETG
                     NADRALAEWWTAFQTASAEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGTD
                     WE"
     gene            complement(11217..12917)
                     /gene="recJ"
                     /locus_tag="ACFB64_001009"
     CDS             complement(11217..12917)
                     /gene="recJ"
                     /locus_tag="ACFB64_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC1209382.1"
                     /translation="MSVKIQTRPVNTDVFNHLLAAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAAGRLADAVERKEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLTALRAELRRRNYFSDGIKEP
                     NLGGLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAARLNNLNIERREIEQSM
                     LRDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTYITDGSLPACDITLEQAQNLACHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRDNLELQLYIDYWEAA
                     "
     gene            complement(12986..13699)
                     /locus_tag="ACFB64_001010"
     CDS             complement(12986..13699)
                     /locus_tag="ACFB64_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209383.1"
                     /translation="MLFRKTTAAVLAATLILNGCTMMLRGMNNPVSQTITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFAVNPEDSAKLTGLLKAGLDKPFQIVEDTPS
                     YARHQALPVKFEAPGSQNFSTGGLCLRYDTGRPDDIAKLKQLEFKAVKLDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFGNILYTPPLLILDAA
                     AAVLVLPMALIAAANSSDK"
     gene            complement(13746..14252)
                     /gene="yjgA"
                     /locus_tag="ACFB64_001011"
     CDS             complement(13746..14252)
                     /gene="yjgA"
                     /locus_tag="ACFB64_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC1209384.1"
                     /translation="MFEQEDEWISKTQMKKQMNGLQDLGMELTRLSNDTLKKIGLDED
                     LYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     QARVRLLADEGALTQFMSDFPNADAGRLRTLVRNTKKEQEQDKPPKNFRALFQELKTV
                     MENGGAEI"
     gene            14406..15737
                     /gene="pmbA"
                     /locus_tag="ACFB64_001012"
     CDS             14406..15737
                     /gene="pmbA"
                     /locus_tag="ACFB64_001012"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC1209385.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALDTDSRIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQSTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGTLSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLGSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(16036..16239)
                     /locus_tag="ACFB64_001013"
     CDS             complement(16036..16239)
                     /locus_tag="ACFB64_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC1209386.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            16484..16786
                     /gene="clpS"
                     /locus_tag="ACFB64_001014"
     CDS             16484..16786
                     /gene="clpS"
                     /locus_tag="ACFB64_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC1209387.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLSVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            16788..19079
                     /gene="clpA"
                     /locus_tag="ACFB64_001015"
     CDS             16788..19079
                     /gene="clpA"
                     /locus_tag="ACFB64_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC1209388.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDAAVPNV
                     LKLCGADLKAVSEQLAASVAENTPLIPDHLLDTVETRPTLGFQRVIQRAMVHTQSAGK
                     GLAEPLDVLVALMSETDSHAVYFLGLQSVTRFEVLRCIAHGSPDEDEDDGNYFSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALADGLAHQIVNDDIPDALKEAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTGGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVAETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLNNMVYGQEDAIDALVSAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTE
                     VAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHRLLDKK
                     VEAEFTPALRKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLADGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(19119..22217)
                     /locus_tag="ACFB64_001016"
     CDS             complement(19119..22217)
                     /locus_tag="ACFB64_001016"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFC1209389.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPAENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVVVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTGFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKDGNKIIVTTIQKLNNLMKS
                     EDNLPVYHRQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKELSAAENRQALL
                     HPERIREITQYILSRFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKTATIFSFAANEEQNAVGEIVDENFEPEAMDSSAKEFLQAVINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNREVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYTDSQTG
                     EARRGYLDVAKELHERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QNVDAADEDAMKAFQEKYYLSDEDVQEMRKVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEGLNETA
                     AKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGA
                     DI"
BASE COUNT         5347 a         6343 c         5565 g         5023 t
ORIGIN      
        1 attttggcgg tcgtgccgaa aaaagcggag gcgatttcaa ataaattgtt tttcacacat
       61 catcccaagc ggcagacgga gtttgcagtc ggacaaatca ggcaagggcg cgcagagtaa
      121 gtcaaatgcc gtctgaaaac tgtttcagac ggcattttta cccgtccgca aaccatctcc
      181 atcctcacaa gcccttaaaa atccgctata atcgcccgca atttgatttc agcaccccct
      241 ttttgaaatg gcacaaaaaa tccaatccgt caaaggcatg aacgaccttc tgcctgtcgg
      301 gcaaaaagat ttcaaactca cggctgtgtt ttggcaggcg tttgaggata cggtaaaccg
      361 ttggacgcga gcttacggtt atcagcaaat ccgtacgccg attgtcgagc aaaccggttt
      421 gtttgtccgc tccatcggcg aggaaaccga tgtagtcggc aaggaaatgt acaccttctc
      481 cgattcaaac gattctttga gtttgagcct gcgtcccgaa ggtacggcct cttgtctgcg
      541 tgcggtggtc gaacacaacc ttctgtacaa cagcccgcaa aagctgtggt atatggggcc
      601 gatgttccgc cgcgagcgtc cgcaaaaagg acgctaccgc caatttcatc aggtcggtat
      661 cgaggctttg ggttttgaag ggccggatat tgatgcggaa atcatcgcaa tgtctgccga
      721 cttgtgggaa aaattgggta ttcgcgaata cctgactttg gaaatcaaca gcttgggcaa
      781 ccgtgaggaa cgcgcggcac accgtgcggc attggttgaa tatctgaccc gctatgaagc
      841 gcaactggac gaagacagca aacgccgtct gaaaaccaat cctttgcgcg ttttggatac
      901 gaaaaaccct gatttgcagg aaatctgcaa tgccgcgccg cgtttggtag attacttggg
      961 cgaggcttcg caaaaccact atgcacgctt caaggcgatg ttggacggtt tgggtattca
     1021 atatattgaa aattcgcgct tggttcgcgg tttggattat tacaatcaga cggtttttga
     1081 gtggacgacc gacaaactcg gcgcacaggc gactgtgtgc ggcggcggac gttacgacgg
     1141 tttgattgaa gaactcggcg gcaagcctgc gccgtccatc ggctttgcga tgggcatcga
     1201 acggctgctg cttttggtga gcgaatacgg ttcgctggaa gtgaacgccg cgcctgatgt
     1261 ctatgcaatg caccaaggcg aaggggcgga cttgcaggtg atgaaatacg cacaagcctt
     1321 gcgcgcgcaa ggtttcaatg taatgcagca ttccggctat caaagcctga aggcgcaaat
     1381 gaaaaaagcc gacaacagcg gcgcgcgctt tgccctgatt gtcgcgcaag acgaactggc
     1441 ggacggtacc gttacgctca aagacatgaa cggcgcacac ggtcagcaaa ccgtttccgc
     1501 caccgatttg acagatactt tacaacaatg gaagaacgca taaatggcag cccatctcga
     1561 agaacaacaa gagttagaca actttaaata tttttggaaa accacgggca aatggctgtt
     1621 tgccctgatg attttggcgg cactcggcta cttgggatac acggtttacc aaaaccgtgc
     1681 ggcttcccaa aatcaggaag cggcggcggt gctggcaaac atcgtggaaa aggcgcaaaa
     1741 caaagccccg caaagcgaaa tcaatgccga actgtccaag ctccaacaaa gctaccccca
     1801 ttccatttcc gccgcccaag ccacgctgat ggcggcggca accgaatttg acgcgcagcg
     1861 ttacgatgtt gccgaaggtc atttgaaatg ggtgttgtcc aaccaaaaag acagcctgat
     1921 tcaggcgttg gcggcgcagc gtctgggcgt tgtgttgttg caacaaaaaa aatacgatgc
     1981 cgcgcttgcc gcactcgaca cgccggttga ggcggacttc gcccccctgc tgatggaaac
     2041 taaaggcgat gtttatgccg cacaggaaaa aagccaggaa gccttaaaaa actacggaca
     2101 ggctttggaa aaaatgcctc aagattctgt cggtcgcgaa ttgcttcaaa tgaaactcga
     2161 ttcgctgaaa taaataccgt ctgaaatgcc gctctctctt cagacggcat ttcagacaac
     2221 cccatcctac ggcaattccc gtaaaaccat ccgccgtgtc ccaacggaca cctgctcgtg
     2281 ttcagttcta ccgaacggca cttgacacag aaagacccaa tcatgaaacc aaccatcgcg
     2341 cttatcggcc gccccaacgt cggcaaatct accttgttca accgtttgac gcgcaccaaa
     2401 gacgcgctcg tgcatgacct gccgggtctg acccgcgacc gccattacgg acacggcaaa
     2461 gtcggcagca aaccttattt tgtcatcgat accggcggtt tcgagccggt tgtggacagc
     2521 ggcattttgc acgaaatggc aaaacaaacc ttgcaggctg tcgatgaagc cgatgcggtt
     2581 gtgtttttgg tggacggccg taccggttta acaccgcaag acaagattat tgccgaccgt
     2641 ttgcgccaaa gtccgcgtcc cgtttatttg gccgtgaata aaggcgaggg cggcgacagg
     2701 gccgtacttg ccgccgagtt ttacgagctg gcattgggcg agccgcacgt tatttccggc
     2761 gcgcacggcg acggcgtgta ttacctgatt gaagaaattt tagaaaactt ccccgagcct
     2821 gaagccgaag aagccgatgc aaaacatccc gtttttgccg ttatcggtcg tccgaacgtc
     2881 ggcaaatcta cgctggttaa cgccattctc ggcgaaaaac gcgtcatcgc cttcgatatg
     2941 gcaggcacaa cgcgcgacag catccacatc gatttcgagc gcgaaggcaa accgtttacc
     3001 atcatcgata ccgcaggcgt gcgccgtcgc ggcaaagtgg atgaagcggt ggaaaagttc
     3061 tccgttatca aagctatgca ggcggttgaa gcggcaaacg ttgctgtttt ggtattggac
     3121 gcgcaacagg acattgccga ccaagatgcg acgattgccg gttttgcttt ggaagcggga
     3181 cgcgcgctgg tagttgccgt caacaaatgg gacggcatca gcgaagagcg gcgcgaacaa
     3241 gtgaaacgcg acatttcccg caaactgtat ttcctcgatt ttgccaagtt ccactttatt
     3301 tccgcgttga aagagcgcgg tatagacggt ttgtttgaaa gcattcaagc tgcctacaac
     3361 gcggcgatga ttaagatgcc gacgccgaaa atcacgcgcg tattgcaaac cgccgtcgga
     3421 cgccaacagc cgccgcgtgc cggcttggtg cgtccgaaaa tgcgttatgc ccaccaaggc
     3481 ggcatgaatc cgccggtgat tgtggtacac ggcaattcgc tgcacgcgat ttccgacagc
     3541 tatacgcgct atctgaccca gaccttccgc aaagccttca acctgcaagg cacgccgttg
     3601 cggattcaat acaatgtttc ggaaaacccg tatgaaaatg cggaagacaa accgaagaaa
     3661 aaaccgctgc gccgcgtcag cctgagcaac cgtattgaga aacgcgaagg ccgaaaggaa
     3721 gagaaaaacc gcttcaagaa gaaaaccaaa gtcagtgtga aaaaacaatt cagcaaataa
     3781 ttcccgacat ttcaaacaat aggaaacatt atgtggttca agcagattag tttttatccg
     3841 ctcaacaaag aaaagctgcc tgaggcggac gtacttgccg ataaacttgc tgaagctgaa
     3901 tttacccatt gccaaggttt ggactggttc agcgaaggct ttaccgcacc ggtttctttt
     3961 tcgcccgaac tcgttttccc tgccgacttt accttgcgcg tcgccctgaa aaaagaggaa
     4021 aaagtcctgc ctgccggcgt catccgcgat attttggaag agaaggtagc ggaaatccaa
     4081 aacaatgaag cccgcaatgt cggtcgcaaa gaaaaacaag agcttaaaga gcaaattaca
     4141 gacgacctgc tgccccgagc gtttacccgc agcagccgta cagaagcggt gtttaacacc
     4201 cgccacggct acctgctcgt caataacgcg gcttccgcca aagcggaaaa catcctgacc
     4261 aagctgcgcg aagctttggg aggtttggaa gcctcgctgc cgaataccaa acaatcgccc
     4321 tcttccctga tgaccggctg gctgttgcaa gggcattgcg aaggcggttt tgaattagac
     4381 agcgattgcg aactcaaagg tacgggcgat attgttcccg tcgtcaaagt atccaaacaa
     4441 gacttaaccg ccgacgaagt ggttcaacac gtcaaaaacg gtaaaaccgt tacccggctc
     4501 ggtttggttt ggcgcgaaca aatcgccttt atcctcactc aagacttcac actcaagcgc
     4561 atccaatacc tcgacgtatt gcaggaagaa gccgaaagca acggcgacga tgccgccggc
     4621 cttgccttcg cctcgcaaat cctgatggcg gaatccgtca gcaccatgtt ggaagagctg
     4681 gtttcctatt tgggcggctg gcaagattga tttttcagac ggcttttata ccgtcagcca
     4741 aaatccaatg gaataaccct tatgcttaaa catctcgcat tcctactgcc cgccatgatg
     4801 ttcgccctcc ccgcccagac cgccgtccta agcccctatc aggaaaccgg ctgcacctac
     4861 gaaggcggga tcggaaaaga cgggcttcct tcaggcaaag gcatatggcg ttgccgggat
     4921 gggcgcggtt ataccggttc attcaaaaac ggcaaattcg acgggcaagg cgtttatacc
     4981 gttgccgccg gccgcgaagt atttctcgag ccgttcaatt ccgacagtac caaattccgc
     5041 aatatggcat tgtcgggcac gttcaaacaa ggcttggcac acggcaggtt cgccgcctcg
     5101 caaaacggcg aaaccctctt ttattatgaa atgcgaacac ggcatgatta aagaagtgaa
     5161 actgcccaaa aacaaataac cccccgtttt tcaaacagca tcccccaatg ctttatccga
     5221 acaccgcaaa aaacaaggaa accccatgag catcaagtcc gacaaatgga tacgccgaat
     5281 gagcgaagaa ttcggcatga ttgatccctt cgagccgaac caaatcaaag aagccgacgg
     5341 acagcgcatc atctcctacg gtacgtccag ctacggctac gatatccgct gcgccaatga
     5401 atttaaaatt tttaccaaca tcaacagcac catcgtcgat cccaaaaact tcgacccgaa
     5461 aaatttcgtt accgtcgaag acgactgctg catcatcccg cccaattcct tcgcactggc
     5521 gcgcacggtc gaatatttcc gcattccgcg caacgtcctg accgtctgtt tgggcaaatc
     5581 cacctacgcc cgctgcggca tcatcgtcaa cgttaccccg ttcgagccgg aatgggaagg
     5641 ctacgttacc ctcgagtttt ccaacaccac ccccctgccc gccaaaatct acgcaggcga
     5701 aggcgtggcg caagtcctct tcttcgagag cgacgaagtg tgtgaaactt catacaaaga
     5761 ccgcaacggc aaatacatgg gacaaaccgg cgtaaccctg cccaaagcct gaaatacaaa
     5821 atgccgtctg aacccttatc gtcgggtttc agacggcatc tacctattta tccgtggtta
     5881 gaactgaacc atcccattga tgctgatgct gatttcctcc acacccggtg cggctgaatc
     5941 cgtacccttc atattgacgc ttgccgccat cggcatcgcg cgcagcattt ttgcccgaac
     6001 agccccatcg cccgcaatat ggctgccgat ttgcccaaaa ttcaatttga cgattttata
     6061 accggacgca cccagaacgc ccgccagttt ttcggcacgc gccttgaaac gcaaaacggc
     6121 atccttgctg acctgatcga tgacctcgtt tcggcgttcg cgcgacacgc tgaaatcggt
     6181 atcttcaagc gaagcatccg tctgaacatc agcaataaaa cggtttaaag catcaaaatc
     6241 cctgccctcc gccttaaatt ccgcacgctc ctcccagcct gtttgaatgc gtctgccgtt
     6301 ggtatattga tagcgcggca tcgcactgcg cgataccaat tcggttttaa agctaccatt
     6361 tttcgatttt ctggtgaaat tgttgaattt tttaacaaac tcggcattga cggcattttt
     6421 gtcccgtcct tccgccgcca cctggaaacg cgcggacatt gtatcctgag ccacctcgat
     6481 acccgccgat tcggaaaatt cgacaatatt gtaattcaat gcctcagccg ccgccggaaa
     6541 agataccgcc agcagggaag ccgccaaaac agaacgcagc atatccgtat ccttacttga
     6601 aaaaccgcca atctaacata taggtacgct aaattgcgtt agcataagac aacaatttaa
     6661 atattcgatc ggtctgcctt ataataaccg tctttccgat actgaatcat attatgtccg
     6721 aaccgttgca cgtcctcgtc atccccttat ggtatccacg atccgaacag gatgtggacg
     6781 gaatattttt ccaaaatcaa gcacaggccc tccaacgcaa aggcatcaaa accgccatcc
     6841 tcgcccccat gttccgttat ctgcggaaag aaacagcaag catcctgacc ggtccttacg
     6901 gttttgcccg ataccggcaa agcggtttgg acatctacgc atggcgcagc atgtatttct
     6961 tccccgcttt ccgtttatcg acatcgaccg catccgctgg gtgcgcgccg gtttgaaagc
     7021 cttcaaacac tacatccgcg aaaacggact gcccgacctg attcacgccc actgcatgaa
     7081 ctatgcaggc atacttgccc aaaaaatttc cgaaaaatac ggcattccct acgttttaac
     7141 cgaacacagc agcaccatca cgcgcggtct gatccgtcac caccaatggc agccgatgga
     7201 aaaagcagcg gcgcccgcct ccgcacgtct cgccgtcagc cgccatttcg cacacgtcct
     7261 gcaacacaaa tacggctgcg aatggcagta cctccccaac atactgggtg gaatattcaa
     7321 gcaaacattt gaataaaaag aaaaaaataa caaaccgcat ttcgtgttct gcaccgtctc
     7381 gcaccttcgc cgcctcaaag gacacgatgt cctgctcact gcttttgccc gggcgttggc
     7441 acaatgcccg caactgcgcc tgaacatcgg cggcagcgga caggaagaac agcggctgaa
     7501 acagcaggcg gcagacttgg gcatcgccca tgccgttaca tttttaggcg cactgcaacc
     7561 cgaagcaatc ttggatttga tgaggaacag cgacgccttc atcctcgcca gccgcaccga
     7621 aaccttcggc gtggtctata tcgaagccct ctcgcaaggg ttgcccgtca tcgccataca
     7681 ttgcggcggc gcggaatcca tcgtttcaga cggcaacgga tacctcgttt ccgttgacga
     7741 tgccgatgtc ctagcctttc tcaaaatata tgaacaccat gccgattttt ccgccgaaca
     7801 gctcagaacc gactgcctcg caacattcgg cgaagatgcc gttatcggca gactgattgc
     7861 cgtattccgt caggcggcgc ggaacacggc gcgaaaacgc ccgaaaaata gattaaaatg
     7921 acgcaaaaca accttcggat tcaaatatga cccataccgt ccacctgcac ttggaagaaa
     7981 ccgacaaccc gacgctgcaa cgcctgtgcg gttctttcga cagcaacctc gattcgctcg
     8041 ccaaagcact cgacatccat atcagccgcc gtttcgagca ttttaccttc aacggtgcat
     8101 ttgcacacgc cggcaaacgc gcactgctca aactcttgga aacggcgcag acgcgcgacc
     8161 taaacgacag catcatccgc cttgccgccg tcgaaaccca aaccgaagat gccgggcatc
     8221 aggaaaaaaa tcacgaacac gcctattatt tccgaaccaa gcgcggcagc ataggcggca
     8281 gaacgccacg gcagaacggc tatatccgcg ccctgctcga ccacgacatc gtattcggcc
     8341 tcgggccggc aggtacgggc aaaacctatc ttgccgtcgc cgccgccgtc gatgcgatgg
     8401 aaaaacacca agtcgaacgc atcattttag tgcggcctgc cgtcgaagcc ggtgagaaac
     8461 tgggcttcct gcccggagac ctgacccaaa aagtcgaccc ctacctccgt ccgctttatg
     8521 atgccctcta tgacctgatg ggctttgacc gtgtaaccaa gctgattgaa aaaggcctga
     8581 ttgaaatcgc cccgctcgcc tatatgcgcg gcaggacgct caacggcgca tacatcatcc
     8641 tcgacgaagc gcaaaacacc acgcccgaac aaatgaaaat gttcctgacc cgcatcggct
     8701 tcggcgcgaa agccgtcatt accggcgaca ccagccaaat cgacctgccc aaaaacatca
     8761 aatcgggatt gaaagacgca cgtgagaaac tgcacggcgt ggcaggactg tatttccata
     8821 ccttcaccgg cgaagacgtt gtccggcatc ctctggtgca aaaaatcgtc gaagcctacg
     8881 aatcggcaga acacgactga cactccaatg ccgtctgaaa cccgatgcat ggaaaatcgg
     8941 gcagagaaaa acagagacgg aagccaagct ccgtatccgt ttctttatcc gtccaaccga
     9001 tacatcaatc gaattttctg catcggattg cctcatctcc gcaaaacatt gccgaagttt
     9061 cgcggttgca cgcctgacgc actttctgcc gcgccacgcc ttcattccga tcgcttgcct
     9121 caaacgtctt ctgaaaaggc gtaacgctgc atacatacat ggtggaattt gaaccgaaaa
     9181 ggtcgatttt cacggtattg ccgccgcttt ccaatacggc gacacggtgt tccaactgcg
     9241 ccagacgtgc ttccaaatcg ctgattctgt cggcaaatgc aggcatggcg gcacacgcgg
     9301 tcaaaatgcc caaaatacat tttttcatca atttttcctt gttgcttgaa ccctgcccac
     9361 ccggacatcc tttttccggg cggcagggtc agactttatt tgcagggatg aagcccctta
     9421 caaatcagaa taatacatga atcggcactt tatgcttcat cctatgtgtt gaaacttcat
     9481 tgctccttgt ctgttgaccg ttattcccaa tccgttccca caaccttcgg cttgggtttg
     9541 cgcctgcgac ggcggcgttt tttcgcctgt ccttcgtttt tttcgtgccg ggcggcggcc
     9601 tcgtttttgg tcatttccgt ccgctgttcc gccgatgccg tctgaaacgc cgtccaccac
     9661 tcggcaagtg cgcggtccgc attgccggtt tcggcgcgca agagcaggaa atcataggcg
     9721 gcacggaaac gcgcctgtgc aaacagtttg tgcggacgtg cgcctttgcg gttttcaaac
     9781 tgcggctgga acatccaaat ttcacgcatc gtggcggaaa agcgttgcgg tacgccccaa
     9841 ccgcgttcga cggtttcgcg catcgtattg attgcatcgg acagggcggg cgtaggtttc
     9901 aaaccctgtt gcagattgct tttccaatgg cggtccaact ctggccacat cagggccgcc
     9961 agtacgaaac cgaccgaaac cgatttgtcg gcacgcagcc gctcatcggt atttttcagg
    10021 gcaagcgccg tcatttttcc ggcgatgccg tctgaaacgc gcaaagcatt gagcagcgga
    10081 tggatgtccg gtatgtcaaa tccgttcaaa cgtttcagac actcgcgcgc gtgccctgaa
    10141 aacagcagtt tcatgatttc gtcgaacagc cttgccacag gttcgtgctt cagacggcat
    10201 atcgattcgg caatcggtgc ggcggtttct tccgacagct caaagcccaa tttgcccgac
    10261 aggcggatgg cgcgcaaaat cctgacaggg tcttcctgat agcgttcggc ggcattacca
    10321 atcataacca gcctgcgggc ggcaacatcg gcaatcccgt tgtggaaatc caaaatctct
    10381 tctttttcag gatcgtaata caaggcattg caggtaaaat cgcgccgcat cgcatcttct
    10441 tcgatgctgc cgtaggtgtt gtccttcatg attctgccgt gcgcgttctg atgaaccttc
    10501 gcaccgccgc gaaacgtcgt tacttcgata atctccgcac cgttcatcac atggacaatc
    10561 tgaaaacgcc tgccgatgat gcggctgcgg cggaagagtt tgtgcacctg ttcgggcgtg
    10621 gcatcagttg cgacatcgaa atctttgggt tcgatgccga gcagcaggtc tctgaccgca
    10681 ccgccgacca cataagcctg aaaccccgcc cctttcaggc ggcgtatgac gttttcggcg
    10741 gcaaagctca acatttcggc acggatgttg tgtctttcgg caggaatgac cgttttactt
    10801 tccgcttttt tactgctccg accggaaggc agcatcttat tcaaccattt tttcagcata
    10861 aatattctga cccgaagagc ggcatctcca cgggcaaccg tgttcagact gcatcaggcg
    10921 gtacgattcc acaagatgcc gaaaaccata agcaaaaggc gtagaaacta cgccgtccga
    10981 atcacgccgc cctcgcggcg gcaacgcgtc attataacag attgcccctt aaaaaatcag
    11041 accctgcttt tgtggcggag tctgaaattt gatgcacctt atatattgcg tccctaagaa
    11101 gggatgatta acaaaaatta acacccttta ctttcttttc cgattgggaa cattcccgtc
    11161 gtacaaacaa gcaaatgaag gaacagaaat cacattcaaa cggcgttccg ccgcctctac
    11221 gccgcttccc agtagtcgat ataaagctgc aattcgaggt tgtcgcgcca ttcgttggca
    11281 acggggcggt acaccgtgcg gatgtattcg ggaatgtctt cgctgcaacg ccagaacatc
    11341 gcttcaaatt cgcagccgtc tttttgcaac cacgcttttt tgtgtttgcc ttccgcgccc
    11401 aaaggctgct ggcgaacgac gtggaactcg tcggtaaagc tcggcggcgc aaagccctgc
    11461 ccccaaacgt ggcaggcgag gttttgcgcc tgttccaacg tgatgtcgca ggcgggcagg
    11521 ctgccgtcag taatataggt ttgcgacaaa tcgtcttcgc acaccatctc acgcacggct
    11581 tcttcaaagg ccgtctgaaa cgcgggaata ttgtgttcaa gtatgcttaa acccgccgcc
    11641 atcgcgtgtc cgccgaattt caaaatcaaa tcgggatggc gtttggacac caagtccaaa
    11701 gcatcgcgca agtgcagatt gggaatggag cgtcccgaac cgcgcacttc gccgttgtcc
    11761 gccggcgcaa acacgatggt cggacggtaa aaacggtctt tgaggcggct ggcaacaatg
    11821 ccgaccacgc cttgatggaa gtcgtcgcga tacgccacca aagtcgtctg acctgaaggc
    11881 agggtttcgg gaaaggcgtt caacgcgtct cgcagcatag actgctcgat ttcgcggcgt
    11941 tcgatattga ggttgtttaa ccgagccgcc agttcctgcg cttcggaatc atctcgcgcc
    12001 aacaggcagg cgatgccgac cgacatatcg tccagccgtc cggcggcgtt gatgcgcggg
    12061 cccaacgcaa agcccatatc aaacggctga gccttgcgcc aatcgcgccg cgccacttca
    12121 aacaaggcgc ggatgccggg gcgcattttg cccgaacgca tccgtttcaa accttgcgac
    12181 acgaggatgc ggttgttgtg gtcgaggggg acgacatcgg cgacggtacc gagtgcgacc
    12241 aaatccaaaa ggccgcccag attcggctct ttgatgccgt ctgaaaaata attgcggcgg
    12301 cgcagttcgg cacgcaacgc cgtcaacaca taaaaaatca cgcccacgcc cgccaagctt
    12361 ttgcttggaa aaccgcagcc tttttggttc ggattgacga tgatgcagtc gggtacggta
    12421 tcggctggca aatggtggtc ggtaacgatg acatccaaac ccagagcctg cgcgcgcgcc
    12481 acgcctgcaa tactggcaat gccgttatcc accgtaatca gcaaatccac gccttgcgcg
    12541 gcggcgattt cggcgagttc gggcgttaag ccgtagccgt gctcaaagcg gttgggcacg
    12601 aggaaatcca ctttcgcgcc catcgccgcc aaaccgtcca aaccgacggc acacgcggtc
    12661 gcgccgtcgg catcatagtc ggcgacaatc aggatttttt ccttgcgttc aaccgcatcc
    12721 gccaaacggc cggcggcggc ttcgcaattc gtcagcgatt gataaggcag gagggaagcg
    12781 agtttgtcgt ccaactcggc aggactttgc acaccgcgcg aggcacaaag ccgggcgatt
    12841 aaaggatcgg cgccggcggc gagcaaatga ttgaaaacgt cggtattgac ggggcgggtt
    12901 tggattttga ctgacatagc gcggggtttt acagtgcggg tgtgcttgaa atgccgtctg
    12961 aaggcttttc agacggcatt gccgttcatt tgtctgagga attcgcggct gcaatcagag
    13021 ccataggcaa gaccagcacc gcggccgccg catccaatat caacaagggg ggcgtatata
    13081 agatatttcc aaacagcttg gatttgtcgg tatgtttttc agtaaccgta taataaatat
    13141 cggcgggcac actttgctca aaatgataat cggcgttcag tttttgcggc gtggcgtagt
    13201 atttgccttt ggcggatacg cagcgcgtgt aaatggtccg attgtcgagt ttgaccgctt
    13261 taaactcaag ctgtttcagc ttggcgatgt cgtcaggtct gccggtatca tagcgcaggc
    13321 aaagacctcc ggtactgaaa ttctggctgc cgggcgcttc gaatttgacc ggcagggctt
    13381 ggtggcgggc atagctcggg gtatcctcaa ctatttggaa gggcttgtcc aacccggcct
    13441 tcaaaaggcc cgtcagcttc gccgaatctt cgggattgac ggcgaaccag tatttcccgc
    13501 ccatcatcac caggctgccc ttttccaatt gggcattgtc ttcggcaacc acaccgaagg
    13561 cgcggatttg gtctttgtca acgtgtttgc gggtaattgt ttggctgacc gggttgttca
    13621 tcccccgcaa catcatcgta cagccgttca gtatcaaggt tgccgccaaa acggcggcgg
    13681 tcgttttacg gaacaacatt ttatcttcca atctaaataa cggcggatgc cgtctgaaaa
    13741 tatgcctaaa tttccgcgcc cccgttttcc atcacggttt tcaattcttg aaacagggcg
    13801 cggaagtttt tcggcggttt gtcctgttcc tgttcttttt tggtgttgcg gacgagcgtc
    13861 ctcagcctgc ccgcgtcggc gtttggaaaa tcggacataa actgcgtcag cgcgccctcg
    13921 tctgccaaca accgtacacg cgcctgttcc acgcgttgca aaaaggcgtt gtgcgccgca
    13981 tcgtcgccgc gcagcttggc aaggaacgcc tcgatgggcg cgggatcggt atcgcgcatc
    14041 agacgtccga taaattgcgc ctgacgtttg agcgcgccgt tggacgtaat ttttttatag
    14101 gtaacgacgg cctcgtacaa atcttcatcc aaaccgattt ttttcagcgt gtcgtttgag
    14161 agcctggtca gttccatacc caaatcctgc aaaccgttca tctgcttttt catttgggtt
    14221 ttgctgatcc actcgtcttc ttgttcaaac atcttgattc tcgaattttt tccaatagcg
    14281 tattttaaca gaatgccgcc gcgttcagac ggctttttgc accgcacgcc ctgcaagcgg
    14341 cacgcttttg cccctgccgc ccgaaacggt taaaatgccg caacgctcca ccaagaaaga
    14401 ttgttatgct gttcaaccac accgcttccg aactcctcga cctctgccgc cgcacgctcg
    14461 acttggcaaa agcagcgggc gcgaccgccg ccgaagccga cttcagcgaa tcgctcggac
    14521 aaagcgtcag cgtgcgcttg ggcgaaatcg aacaaatcga gttccagcag gacaaatcac
    14581 tggacattac cgtttacgtc ggcaaacgca aaggccgcgc cggcaccgcc gacttctccg
    14641 aacaagccct gcgcgacacc gtccgcgccg ccgccgacat cgcccgccac accgccgaag
    14701 acggttgcgc cggacttgcc gaccccgagt tgatggcgca acacatcggc gaccccgacc
    14761 tttaccacga atgggatttg gatacggaag ccgccgtcgg cttggcaaaa caatgcgaac
    14821 aggcagcgtt ggacacggac agccgcatcg aaaactccga aggcgcggcg gtgcaaaccg
    14881 gacactacca atacgtttac ggcaacaccc acggttttgc cgcacaccgg caaagcacgc
    14941 accacagcat ttcctgcagc gtcgttgccg ccgacgaaaa cggaatgcag cgcgactact
    15001 ggtacgattc cgcctgccgc catccggata tggacagccc cgaaaccatc ggtcaaaccg
    15061 ccgcccgccg cactttgcgc cgactcggca gccgcagcat cccgacaggc agctaccccg
    15121 tcctcttcga taccaccgtt tcgggcgggc tgatcggaca cctcgtcggc acactctccg
    15181 gcggcgcgct ctaccgccaa agcagtttcc tgatcgacag catcggcaaa aaagtcctgc
    15241 ccgatttcct caacctgcgc gaagaaccgc acatcccccg ctctttccgc agcagctatt
    15301 ttgatgcgga aggtgttgcc accgcgccgc gcttcgtgat tcaaaacggc attgtcgaag
    15361 gctatttcct cggcagttac agcgcgcgca aactcggaat gcagaccacg ggcaacgccg
    15421 gcggcgcgca caacctgtat ttgaaccata cgcacgaaac gcaatccgac ctgctgaaag
    15481 aaatgggcac gggattgctg gtaaccgaat tgatgggaca gggtgcgaac accatcaccg
    15541 gcgactattc gcgcggcgcg gcgggttttt gggtggaaaa cggcgtgatt gcctaccccg
    15601 tccacgagat taccgttgcc ggacgcttgc aggatatgta ccgcgacatc gtcggcgtgg
    15661 cggatgacgc tttgcggcgt tcgtccaaca aaatcggttc gattctgatt gcggggatga
    15721 cggttgccgg aagctgaacg ccctgccgat gccgtctgaa gacgaaggcg gcgcgttttg
    15781 ccccgtgcct gcccgtttgc cgttttatgc cgcaccgcct tttaccggcg acattgcaca
    15841 tttttttcat cagcactgat tttcccttat ccgtcaaaat actaagccac ttgcccacaa
    15901 cagggtacac acaagttcag gataaggcgg gcggggattg gggacttttc cgccatcggc
    15961 aaaacggcag acaaaaacgg cgggacattg cctgtcccgc cgttttcaaa tatacatttg
    16021 accgtacatc aggaattaag ctgcctgaat gttggcggcc tgtttgcctt tagggccggt
    16081 ggttacgtcg aaagagacgc gttggccttc tttcagggtt ttgaaacctt ccatattgat
    16141 cgctgagaag tgtgcgaaca aatcttcgcc gccttcgtca ggagtgatga aaccaaaacc
    16201 tttagcgtcg ttaaaccatt ttacgatacc ggttgccatt agaaacttcc tatacttaaa
    16261 aattaacaaa atcagcaaaa taaggcatac agcaaactta aacgttcagc gtttccaccc
    16321 agctttatat acttggtcga gtgccgactt ttgcttaacc gtctttttac atttgttaag
    16381 acaaaacgtc aagctatgtt ttcaaaaaag tgggaaaata ggcgaaagta ccgaaaatac
    16441 aacaaaaatg ccgtctgaac gcggctttca aatgggacac gctatgaccg cgcaacacca
    16501 atccgacacg cttttgcacc gcctgaacac cctgccgccg aaacgttacg gcgtattttt
    16561 attgaacgac gattacacca cgatggaatt tgtcgttgaa atcctgaccg aaatctttat
    16621 gctcgggcag gaacaggcgg tagcggtaat gctctcggtt catcacgaag gcaaaggcct
    16681 gtgcggcact tacacgcgcg atatcgccca aaccaaacag caacaagtca tgcagcgggc
    16741 aaaagccgaa ggacatccgc tgcaatgtat tgtcgaggag atttaatatg cttgcccctg
    16801 aattggaaca gattttgcag cagctttacc gcgaggcgcg taaggctcat tatgaattta
    16861 tcagcctcga gcatctgctt ttggtactca tcgaagaaga tgccgccgtg ccgaacgtct
    16921 taaaactctg cggtgcggat ttaaaagcgg tgtccgaaca gctcgccgcc agcgttgccg
    16981 aaaacacccc cctgattccc gaccaccttt tagacacggt cgaaacccgg cccacgctcg
    17041 gcttccaacg cgtcatccag cgggcgatgg tgcataccca atcggcaggc aaaggcttgg
    17101 ccgagccttt ggacgttttg gtcgcgctga tgagcgaaac cgacagccac gccgtttatt
    17161 ttttgggttt gcaatctgtt acgcgttttg aagttttgcg ctgtattgcc cacggctctc
    17221 ccgatgaaga tgaagacgat ggcaactatt tttcagacgg catggacgac gataatgaga
    17281 accgcaccaa accgggcaaa aaccctttat cggcgtacac cgtcaacctc aacgccgaag
    17341 tcaaagccgg ccgcatcgac cctttgattg gtcgcaaaca cgaaatggaa cggctggtgc
    17401 aaatcctgtg ccgccgccgc aaaaacaatc cgcttttggt cggcgaagca ggcgtgggca
    17461 aaaccgcgct ggcggacggt ttggcacatc aaatcgtcaa cgacgacatt cccgacgcgc
    17521 tcaaagaggc cgaagtgtac gcgctggata tgggttcgct tttggcgggc acaaaatacc
    17581 gcggcgactt tgaagcgcgg gtcaaatccg tcttgaaaca gctcgaaaaa atcccgcacg
    17641 ccattttatt catcgacgaa atccacacca tcatcggcgc gggcagcacc ggcggcggca
    17701 cgatggacgc gtccaacctg ctcaaacccg cattggcgaa aggttcattg cgctgtatcg
    17761 gcgcaaccac ttacgacgaa taccgtacta tttttgacaa agaccacgcc ctaagccgcc
    17821 gcttccagaa aatcgacgtg gtcgaaccca ccgttgccga aaccgttcaa atcctgcgcg
    17881 gcttgaaacc gatgttcgaa gccttccacc aagtccgcta cacgcaaggc gcactcgaag
    17941 ccgccgccga actctccgcc cgctacatca acgagcgctt cctgcccgac aaagccatcg
    18001 acgtaatgga cgaagcaggc gcggcgcaac ggattctgcc caaatccaaa cagaaaaaag
    18061 tcatcggcaa agcgcaaatc gaaaccgtca tcgccaaagt cgcgcggatt cccgaaaaga
    18121 ccgtgtcgca cgacgacaaa caagtgttgc aattcctcgg ccgcgatttg aacaatatgg
    18181 tttacgggca ggaagacgcc atcgacgcgc tggtttccgc cgtcaaaatg tcgcgttcgg
    18241 gcttggggct gcccgacaaa ccgataggca gcttcctctt ctccggccct accggcgtcg
    18301 gcaaaaccga agtcgccaaa cagcttgcct actcaatggg cgtaccgctg caacgctttg
    18361 atatgtccga atacatggaa cgccacgccg tatcgcgact catcggcgcg ccaccgggct
    18421 acgtcggctt tgaacaaggc ggccttttga ccgaagccgt caacaagcag cctcattgcg
    18481 tgttgctctt ggacgaaatc gaaaaagccc atccagatat tttcaacgtc ctcctgcaag
    18541 tcatggacgc aggcaaactg accgataaca acggtaagag cgccgatttc cgcaacgtca
    18601 tcctgatcat gaccaccaac gcaggcgcag aaagcctcag ccgacccagc ctaggcttta
    18661 ccgccaaacg cgagcgcggc gacgaaatgc aggcaatcaa caaactcttc acgcccgagt
    18721 tccgcaaccg cttggacgcg atcatcccgt ttgcaccttt gtccgaaccc atcatcgtca
    18781 aagtcgtgga caaattcctg ctccagctcg aacaccggct cctcgacaaa aaagtcgaag
    18841 ccgaattcac accggcattg cgtaaatact tggcagaaaa aggtttcgac ccgcaaatgg
    18901 gcgcgcgccc gatgcaccgc ctgattcagg aaaaaatccg caaaccgctc gccgacgaac
    18961 tcctgttcgg caaactcgcc gacggcggct ttgtgcggat agactgggat gcggcgaaag
    19021 aagaagccgt attgaagttt aagaaaagca aggtcaaaat aaaaaccgcc tccgcataaa
    19081 atcaaaaatg ccgtctgaaa ggttcagacg gcattttgtc aaatatcggc ccctattccg
    19141 gcaaacttct ccacaaacgc cgcgattttt tggaagacgc tttgtttctt cgtcagatat
    19201 tgcgggttga gcggactcat tttcggcagg gtttcggtca gttccgtacc gttttcgctg
    19261 gcatagccgc gtttgagcga gccggtcaaa tagcgtttgg cggcggtttc gttcaggcct
    19321 tcggcggcaa tcaatcctgc cgcttcgtgc cgcatcacct cttgcgcgta ggtgtaaaag
    19381 gtttccagta tggcgggaac gtcgggcact ttatccaaat ccgtatcgtt gatgaaatcc
    19441 acaatcaggc cctctttggc gcggtggccg atgctggcgc ggatgatgcg gcggatttct
    19501 tccaccagct ccgctttgcc tttgattttt ttgtgatgct cgaaaaccag ttgcaggatg
    19561 taatccagat tgatttcctg cgatttgagc aaatccacct caaaaaccac atcgtcccag
    19621 tcgatttttg actgttcttt ctgttcgcct gctttttggc ggcgcagcca gtctcggatg
    19681 tcgttatagg cggaacggta gtcctgcacc gccctttcag acggcatcgg cactttccgc
    19741 atttcctgca cgtcttcgtc gctcaggtag tatttttcct gaaacgcctt catcgcatct
    19801 tcgtccgccg cgtccacatt ctgcaattcg cgcagcgcgg caaattcatc gtagttctgc
    19861 aatacatttt ccgcccgcag gtattcgccg aagagtttgg caaaatcttt tttgtctttt
    19921 tccgtttcga ttttgtcggg atcggggaaa cgctcgtgca attcttttgc tacatccaga
    19981 taaccgcgcc gtgcttcgcc ggtctggctg tcggtatagc cgttcatgta ttcttcgtag
    20041 cttttttcca gcactacatt tttggtgttt ttgtcgccga agagcgtgat cgcatcaatg
    20101 gttgcctgct ccaagtcgcg gaagcagaca atgttgccga aggttttggt agcatcgtaa
    20161 atgcggttgg tgcgagaaaa cgcctgcatc aggccgtgat agcgcaggtt tttatcgacg
    20221 aacagcgtat tcagcgtcgg tgcgtcaaaa cccgtcaaaa acatgccgac cacaatcagc
    20281 aagtccactt cccggttttt cacccgtttt gccaaatcgc ggtagtagtt ctgaaaggct
    20341 ttgctgtccg tgccgaaatt ggttttgaaa caggcgttgt aatcgttgat gacagcctgc
    20401 aaaaattctt tcgcgctgct gtccatcgct tccggttcaa agttctcatc gacaatttca
    20461 ccgacggcgt tttgctcttc attggccgca aaggaaaaaa tggtggctgt tttcaacggg
    20521 tgcaggctgc ctgcctgttg tgttttgaac gcttcgtaat agcacttcgc cgcatccacg
    20581 ctgctgacgg caaacatggc gttgaagcct ttgccgcccg cattcaagcg gtgcgttttc
    20641 tgcctgaacc ggctcaggat atattgcgtg atttcgcgga tgcgttcggg gtgcagcagg
    20701 gcttggcggt tttcggcggc actcagttcc ttctcgtcct gttccgcttc cacggctttg
    20761 aactgcgggc gcacatcgtt gtaatccact ttgaatttca atactttttc atcgcggatg
    20821 gcatcggtaa tcacataaga atgcagctcc cgcccgaaca cgcccgccgt ggtttccgcg
    20881 cccaaagcgt tttcgggaaa aatcggcgtg ccggtaaagc cgaactggca gaatttttta
    20941 aatttctttt tcaggttttt ttgcgcttcg ccgaattgcg agcggtggca ttcgtcgaaa
    21001 ataaatacaa cctgccggtg ataaaccggc agattatctt cacttttcat caggttgttc
    21061 agcttttgga tggtggtaac gatgattttg ttgccgtctt tttccaaatt gcgtttcaag
    21121 cccgccgtac tttccgagcc gtttacgctg tcgggcgaaa aacgttggta ttccttcatc
    21181 gtctgatagt ccaaatcctt cctgtccacc acgaagaaaa ccttgtcgat aaacgccgat
    21241 tccgtcgcca gacgcgccgc cttaaagccg gtcagcgttt tgccgctgcc cgtggtatgc
    21301 caaacatagc cgccgctttc cggcccgctc caattcttcg cctgcgccga gctgttgatt
    21361 ttccacaaaa tgcgttcggc ggcggcaatc tggtacggcc gcataatcag cagcgtatca
    21421 ttcgcatcga acacgctgta atgcagcaaa acgctcagca atacgctttt ctgcaggaac
    21481 gtggcggtaa agtctttcaa atccttaatc ggatgattgt ccgaccgcgc ccaattcatc
    21541 gtgaaatcga agctgttttt gtcgcgcttg gtggtgttgg cgaaataacg cgtgtccgtg
    21601 ccgttggaaa tcacgaagat ttgcaggaat ttgaacagcg aattttcgct gttgaagctc
    21661 tctttgctgt aacggtgcac ctgattgaat gcctcgcgca ccaccacgcc gcgctttttc
    21721 aattcaatct gcaccagcgg caggccgttt accaacacgg taacgtcata acggtttgca
    21781 tgcgtgcccg tctgctcaaa ctggttgata agctgcacat ggttgcgggc aaggtttttc
    21841 ttgtccagca gataaatgtt tttcagacgg ccgtcatcaa aagcgaaatc gtaaatatgg
    21901 tcgtcgtgga ttttgcgggt tttatcggta atgttttcag ccggcctgtc cagatattcc
    21961 gtcaaaaacc gcgcccattc gccgtctgaa aacgccacat cgttcagccg ctgcaactgg
    22021 gcgcgcaggt tttccagcag cctgctttgg ctgttcaaat ccttgcggta ttcgtacccc
    22081 tgattctgca aatcggcgat taactccgct tccaaccggt tttccgattg gtagctgccc
    22141 gactgttcga ttttttcata ttggtcgagc acgatgaaat tcggcgtttc ggcgatgggt
    22201 ttggtttcga ggttcatggg tcgtcctttg cggtgtcggg ttttatagtg aattaacaaa
    22261 aaccggtact gcgttggc
//
LOCUS       DAXDXW010000031        20762 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXDXW010000031 DAXDXW010000000
VERSION     DAXDXW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20762
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.032"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(102..869)
                     /locus_tag="ACFB64_001017"
     CDS             complement(102..869)
                     /locus_tag="ACFB64_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC1209390.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPKSRNRAV
                     IALGKGALAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVAGDADVAV"
     gene            complement(957..1784)
                     /gene="mutM"
                     /locus_tag="ACFB64_001018"
     CDS             complement(957..1784)
                     /gene="mutM"
                     /locus_tag="ACFB64_001018"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC1209391.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVILRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEERHPLLEKLGPEPLSEAFCTDYLYAGLKAQKRAV
                     KLALMDNTVVVGVGNIYANESLFRAGISPHRPANRLKKKECAVLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCLRCGGLVVKETLGQRGTFYCTNCQK"
     gene            complement(1837..2502)
                     /locus_tag="ACFB64_001019"
     CDS             complement(1837..2502)
                     /locus_tag="ACFB64_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFC1209392.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFNRYVASYRFSGMCAVQVGGQWL
                     SLSEDIVCVPRDMSMSAENMALADVFADMLLLPHTLECGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFCRWFDGVRLPEKRFCLPLPELKRQLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYWGGKVLAAGAAR
                     VAD"
     gene            2681..4657
                     /locus_tag="ACFB64_001020"
     CDS             2681..4657
                     /locus_tag="ACFB64_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC1209393.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQVGLAIMRLNSSILD
                     LPPTKQYFQSGSLWDELRQGFRMGEVNPELVRRHESKFIASRSYFDRVVNRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAVNRARDQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPGRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKNKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLHTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVSIARIQPAAAQTADITVAPLPQETVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQVVPPNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAETSPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4725..5417
                     /locus_tag="ACFB64_001021"
     CDS             4725..5417
                     /locus_tag="ACFB64_001021"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC1209394.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     YVETLDIDGEAVTVVPEPYFYILLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVPLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMVAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            5891..7261
                     /locus_tag="ACFB64_001022"
     CDS             5891..7261
                     /locus_tag="ACFB64_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC1209395.1"
                     /translation="MSSPSNTNRQTWSSRLTYILTVAGATVGFGATWRFPYLVGENGG
                     GAYVFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPVAKIWKLVGRMGL
                     LGAFGIMAYYMVLGGWVISYIVNIIGGNLNISSPVDGVVTKGFFTEHIENSPWEIAFY
                     TLLFVAVNQWILVKGVIGGIEKTAKYLMPLLFLFLIAMVVRNVTLPGAMEGVAFYLKP
                     DFSKITAELFVFVLGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAVL
                     AGFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSHMWAGPVFAVIFFSLLLIAALTTSLT
                     IYEVLITTIQEKTKIRRTAAITIVLSVIFVFGNIPSILSYGPWKDVSVFGKNIFDAFD
                     YISGNILFMLTALGSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLI
                     FVSNLF"
     gene            7494..8150
                     /gene="cmk"
                     /locus_tag="ACFB64_001023"
     CDS             7494..8150
                     /gene="cmk"
                     /locus_tag="ACFB64_001023"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC1209396.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSALAKKLPAVFSGNRILLDGEDVSDGIRTEAIGMGASAVAQWP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGFTFERILSDIETRDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            8303..9988
                     /gene="rpsA"
                     /locus_tag="ACFB64_001024"
     CDS             8303..9988
                     /gene="rpsA"
                     /locus_tag="ACFB64_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC1209397.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QATRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            9999..10313
                     /locus_tag="ACFB64_001025"
     CDS             9999..10313
                     /locus_tag="ACFB64_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC1209398.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(10509..10916)
                     /locus_tag="ACFB64_001026"
     CDS             complement(10509..10916)
                     /locus_tag="ACFB64_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFC1209399.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLYLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            11066..12176
                     /locus_tag="ACFB64_001027"
                     /pseudo
     CDS             11066..12176
                     /locus_tag="ACFB64_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
     gene            12185..13012
                     /gene="fghA"
                     /locus_tag="ACFB64_001028"
     CDS             12185..13012
                     /gene="fghA"
                     /locus_tag="ACFB64_001028"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC1209400.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPDNPENQPLG
                     VIYWLSGLTCTEQNFITKSSFRRYAAEHQVVVVAPDTGPRGEQVPNGAAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEEHFPTNGKRSIMGHSMDGHGALVLALRNRE
                     HYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTKDFIETCRAANQPIDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            13542..14693
                     /gene="zapE"
                     /locus_tag="ACFB64_001029"
     CDS             13542..14693
                     /gene="zapE"
                     /locus_tag="ACFB64_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC1209401.1"
                     /translation="MNNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHRRLKALKSESNPLKSVAAEITKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSGFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRTIWFDF
                     RALCFSPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            14752..15177
                     /gene="ndk"
                     /locus_tag="ACFB64_001030"
     CDS             14752..15177
                     /gene="ndk"
                     /locus_tag="ACFB64_001030"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC1209402.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPTEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            15321..16415
                     /gene="rlmN"
                     /locus_tag="ACFB64_001031"
     CDS             15321..16415
                     /gene="rlmN"
                     /locus_tag="ACFB64_001031"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC1209403.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQASIEIPKLMMSQESSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGVNDKAQHAY
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRIFRDILQQAEFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            16418..17179
                     /gene="pilW"
                     /locus_tag="ACFB64_001032"
     CDS             16418..17179
                     /gene="pilW"
                     /locus_tag="ACFB64_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC1209404.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSNPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNVQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            17195..18460
                     /gene="ispG"
                     /locus_tag="ACFB64_001033"
                     /gene_synonym="gcpE"
     CDS             17195..18460
                     /gene="ispG"
                     /locus_tag="ACFB64_001033"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC1209405.1"
                     /translation="MNTLQRRKTHQVLIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLVSSAPKPPEEVMKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVHDLIQVYRE
                     LGSRCVYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGNNIASEFL
                     AIVEEYVKTNYGKNSSKRNKGKVIPIQSL"
     gene            complement(18514..19128)
                     /gene="clpP"
                     /locus_tag="ACFB64_001034"
     CDS             complement(18514..19128)
                     /gene="clpP"
                     /locus_tag="ACFB64_001034"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC1209406.1"
                     /translation="MSFDNHLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLRL"
     gene            complement(19224..20534)
                     /gene="tig"
                     /locus_tag="ACFB64_001035"
     CDS             complement(19224..20534)
                     /gene="tig"
                     /locus_tag="ACFB64_001035"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC1209407.1"
                     /translation="MSVTVEILENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGYPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVDREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEPTLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALIKAVELKVPVALVNEEAARLANEMKQNFVNQGMTDAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADTSRLQAPTSLAVESNVVDFVLGKAKVNKKALSFDEVMGAQA"
BASE COUNT         5452 a         5629 c         4780 g         4901 t
ORIGIN      
        1 aacaaactat ccttacggca tttcttttga ggtactcccg ttaccgcagc cttaaggcat
       61 ccgcaaaagc gaatcatacc gctacattcc gacaaccgct ttcagacggc aacatccgca
      121 tcgcctgcga caacggcacg gatgctttct tcgattttat cttttaaagc ataacggtct
      181 tcgctttccg ccgcgtccgc cacgcaaacg aaatcgactt tgatagtcag ttttttcata
      241 gacacgattc gccacaggca agtcggcaaa ccgacatcgg catacgacgg acgggccgtc
      301 ctttttcccg tttcgtcata ataacgcagt gcgaccgcca aaacctttgc ccccgcatcg
      361 atggcggatt ggaacagcgc agccttgaac ggcaggagcc ccagtccgga ggaagttcgc
      421 gcctcaggga aaaaactgac gttttgaccg cgttgcaagg tttcgcagac ggcgcggtta
      481 atcggttcga tgtcgcgccg cgaattgcgg ttgatgaaca ccgttcccgc gttctgcccc
      541 atcttgccca ataccggcca gcttttgatt tcctgcttgg cgataaagct actcggataa
      601 accgcgctca tcgcgaaaat atccagccag gacacgtggt tggcggcaac caagacaccg
      661 ttcggatgtt cgggtgcggg tctgcccacc tccaatccga tatccaaagc cgccaaagcg
      721 cccttgccca acgctataac tgcccgattg cgcgacttgg ggcagccgcc gtcaataccg
      781 cgcaggtttt tgccggtttt gaacagccag accgtcaagc ggcacaagcg gcgcagacgt
      841 gtaaaaaatg aagctttatt tgaagacatg ggcaattctt tcccgataat cggtgtttgt
      901 tgttgaaacg ttcggacggc ataccgataa aatgccgtca gaaaccgttt tccgccctat
      961 ttctgacagt tcgtgcaata aaacgtgccg cgctgcccca aagtttcttt cacaaccaaa
     1021 ccgccgcacc ggaggcacgg ctgattgtgc cgcccgtaca ccgtatattc ctgttgaaag
     1081 tagccgcttt tgccgtcgct gtccacaaaa tccctcagcg tactgccgcc cgtttcaatg
     1141 gcgcgctgca acaccgcttt gacggtttca accaaaaccg cgcactcttt cttttttagg
     1201 cggttggcag gacggtgggg cgaaatgccc gccctgaaca gactctcgtt ggcataaata
     1261 ttgcccacgc cgaccacgac ggtattgtcc atcagagcga gtttgaccgc gcgtttctgc
     1321 gccttcagcc ccgcatacag ataatccgtg caaaatgcct ccgacaaagg ctccggcccc
     1381 agtttttcca acagcggatg acgttcttcg attccctcat accaaagtat cgcgccgaac
     1441 tttctcggat cgcggtaacg catgaccgtg ccgtctgaaa acacaatatc gacgtgatcg
     1501 tgtctgtccg gcctgccgat acgtccgtcc gacggcgtaa agatccgcaa gctgcccgac
     1561 atccccaagt gaatcagcaa aatccccgtt tggaaacgga caatcagata tttggcacgc
     1621 ctgccgcagg acaaaacctg ccggccggac aaaatctccc ccaaatcggg attgatctgc
     1681 cagcgcagct tcagttggcg caatatcacg gcttccaccg tttttccttc aatatgcggc
     1741 gcgatgccgc gcaacgtcgt ttccacttcc ggcaattccg gcataacccc tcccgacatt
     1801 tcttctgaca gatgccgtct gaaagacggc cgtcccttaa tccgcaaccc ttgccgcacc
     1861 cgccgcaagg actttgccgc cccaataccc gtcccacgcg ggcaggggcg ttacgcccgc
     1921 cgcccttttg accaaaacca aaccgtacac tgcggcacat tgcggccacc aacggtcgcc
     1981 cgccttttcc ataaaccgcc aaaagcgtat ttgcccgagc gacgaaacgg gaggcagata
     2041 caccatgaat ttcccaaatt caatatcgaa accggcatcg gcaagctgcc ttttcaactc
     2101 gggcagcggc agacaaaacc gtttttccgg cagacggaca ccgtcaaacc aacggcagaa
     2161 tccccaaagc gaatacggat tgaaacccgt cagcatcaaa cgtccggacg gtttcaatat
     2221 ccggtgcgct tccgacagga tttgcgaagg tacgccgcat tccagcgtat gcggcaaaag
     2281 cagcatatcc gcaaaaacat ccgccaaagc catattctcc gccgacatcg acatatctcg
     2341 cggcacacag acaatgtctt cagacaggct cagccactga ccgcccacct gaaccgcaca
     2401 cattcccgaa aaacggtatg acgccacata ccgattaaag aagtcctgtt ccaattttgc
     2461 aacataccgc cccatcgccg tatcttcaaa ccatgcatcc atattgcgtc cgtttcaaac
     2521 agattgcctg ccgatatttt attccaaaca ggatttctgt caaaaaacac atcggccgcc
     2581 catttccgaa tccgcataaa gttcctgtaa aacttgacgc tttttcagtc aaacagtacc
     2641 atcggacgat aaaatatgtt tattccgccg agtataaatc atgtccaaac tcaaaaccat
     2701 cgccctgacc gcatcaggtc tgtccgtttg tccgggtttc ctatatgccc aaaacacctc
     2761 atcacaccaa gtcggtttag cgattatgcg gttaaactct tcaatactcg acctgccacc
     2821 gacaaaacaa tatttccaat ccggcagcct gtgggacgag ctgcgccaag gcttccggat
     2881 gggcgaagtc aatcccgaac tggtacgccg ccacgaaagc aaattcatcg caagccgcag
     2941 ctatttcgac agggtcgtca accggagccg accctatatg taccatatcg ccaacgaagt
     3001 caaaaaacgc aatatgcccg ccgaagccgc cctgcttccc ttcatcgaaa gcgcgttcgt
     3061 caccaaagcc aaatcacacg tcggcgcatc gggcctgtgg cagttcatgc ccgctaccgg
     3121 caggcattac ggcttggaaa aaacaccggt ttacgacggc aggcacgacg tttacgcagc
     3181 caccgatgcc gcactcaact atctgcaata tctctatgga ctgttcggcg actggccgct
     3241 cgcctttgcc gcctacaact ggggtgaagg caacgtcgga cgcgccgtca accgcgcccg
     3301 cgaccaaggg ctcgaaccga cctacgaaaa cctgcgtatg cccaacgaaa cgcgcaacta
     3361 tgtccccaag ctgctcgccg tgcgcaacat tattgccacc ccccaatctt tcggcatgaa
     3421 tatcagcgac atagacaaca aaccctattt tcaggcagtc gaaccgggcc gtccgctcga
     3481 caacgaagcc atcgcccggc ttgccggcat cacgcaaagc gagctgctcg ccctgaatcc
     3541 tgcattcaac gtccccgcgt tcatccccaa aaacaaacgc aaactgctgc ttcctgtcgc
     3601 gtccgtccaa accttccaaa gcaactacct caacgccgca cccgacagcc tgttttcatg
     3661 ggaagtctat acgcctgccg ccaaaaccag cctgtccgac atctcgacgg caaccggcat
     3721 gagcattgcc gacatcaaac gcctcaacaa cctgaacggc aaccttgtca acgcaggacg
     3781 cagcatcctt gtcgccaaga acggcaagac ccttcatacg gcatcggaat ccgtcgtttc
     3841 catcgacatc gacaatacgc ccgacaccta ccgttccaat atgccggcag gcacggtgaa
     3901 cgtcagcatt gcccgaatcc aacccgccgc cgcacagaca gcggacatta ccgtcgcacc
     3961 tttgccgcaa gaaaccgtcc gtacggaacc cgatcccctt gtccgcattg ccgaacccgc
     4021 ccttgcgaca gccgcagcgc aacctcaaac cgaaaaacag actgccatgc cgtctgaaac
     4081 ccaaaccgca acactcgcgc aggtcgtccc cccaaacgac atgcaggcgg cagacgaact
     4141 catgcagctt gttgcccgaa acaacctgcg ccggcaggct gaagaaacca tctccgccgt
     4201 catcggcacg cctgacacag ttgccgaaca caacatttcc tcatctccgc aacataccgt
     4261 tgccgccgac ggcaaacgcc gggcacgttt ggaaacgcgc gtagccaaag ctgccgacgg
     4321 cgaagccgaa acctccccgc tccatgccag catccaccgc gttgtagaag gcgacaccct
     4381 gttcaacatt gccaaacgct acaacgtcag cgtagccgac ctgattgtcg ccaacaacat
     4441 caaaggcaac accatccaaa aaggacaggt actccgcctg gcgcaggcag cccctgccca
     4501 aacccgtatt gaaaaagtat cctacaccgc gcgcaaaggc gacaccttca aaagtatcgc
     4561 cgcgcgcttc aatatccata tcgacgacat ccgccggctc aatcccaacc tgaacaccat
     4621 caatccggga cagagggtca aactgattgg aagctgattc ggatacggca catgacagga
     4681 ctttctcagt cctgtctttt tcatcccaca tttcccatac catcatgaaa ctcatcaaat
     4741 acctgcaata tcaaggcata ggaagccgca agcagtgcca atggctgatt aggggcggct
     4801 acgtctccat caacggaacc tgcatggacg acaccgatgc agacatcgat tcctcatacg
     4861 tcgaaacgtt ggatattgac ggggaagcag taaccgtcgt tcccgaaccc tatttctaca
     4921 tcctgctcaa caagcctgaa gattacgaaa cttcgcacaa accgaaacac taccgcagcg
     4981 tattcagtct gttccccgac aatatgcgga acatcgatat gcaggcggtc ggcaggctgg
     5041 atgcggatac gaccggcgta ttgctgatta ccaacgacgg caaactgaac cacaacctga
     5101 cttcgccgag cagaaaaatt cccaagctat acgaagtaac gctcaaacac cctacaggag
     5161 aaacgctctg cgaaactttg aaaaacggcg tgccgctcca cgacgaaaac gaaaccgttt
     5221 gtgccgccga tgccgttttg gaaaatccga ccaccctgct gctgaccatt accgaaggaa
     5281 aataccacca agtcaaacgc atggttgccg ccgccggcaa ccgcgtgcaa caccttcacc
     5341 gccggcgatt cgcacatttg gaaacagaaa acctcaaacc cggggaatgg aaatttatcg
     5401 aatgtccgaa attctgaaac aaaattccat aatttcccta tattcaacca tatgcttatt
     5461 aaataagcag ttttacaaaa attaactcaa aaaacagtta gtttacaata taatccgctt
     5521 gtctaagtaa cgcctgttca agcaggatta tgagcaagac gttttccccg taatgtgttt
     5581 tgccatctat acttcccccc ttgtatagat ggttttctat ttcaaattcc ctctctttca
     5641 tcatcattga aatgctatca ttaatatgat taacaaaatt tcacttccgt acaccgatgt
     5701 agaaaaataa cagattaatc tgttcataaa ctccgattct tcctcatcgg tttatactca
     5761 tcagaaatat tcactgaaat atatgcattt tacatggaaa atcactaggc agttgaaaaa
     5821 cattcagtca atttctgtta agaatcacgt gtaaaatttc cgctttatct caatcggaca
     5881 cggtttcaat atgtcttccc cttcaaatac caaccgtcaa acctggtcca gccgtttgac
     5941 ttatatcctt accgttgccg gcgcgactgt cggtttcggc gcgacgtggc gtttcccgta
     6001 tttggtcggt gaaaacggcg gcggcgcgta tgtgttttta ttctgtatcg cgatgctggt
     6061 tatcggcatc ccgatgattt tggtggaaaa cgtcatcgga cggcgcaaag gcgtgaacgc
     6121 gctggatgcg ttcggcggcc cgatgaacgg caaacctgtt gccaaaattt ggaaactggt
     6181 cggccggatg gggctgctcg gcgcgttcgg catcatggct tattatatgg tactcggtgg
     6241 ctgggtaatc agctatatcg ttaatattat tggaggaaat ttgaatattt ccagccccgt
     6301 cgacggtgtg gttacaaaag gcttctttac cgaacacatt gaaaacagcc cttgggaaat
     6361 tgcgttttat acgctgcttt ttgtcgccgt gaaccaatgg attttggtca aaggcgttat
     6421 cggcggcatt gaaaaaacgg cgaaatacct gatgcctctg ctgtttttgt tcctaatcgc
     6481 gatggtcgtc cgcaacgtta cccttccggg cgcaatggaa ggggttgctt tctatctgaa
     6541 acctgatttc agcaagatta ccgccgaact gttcgtcttc gttttgggac aggtattttt
     6601 tgccctgagc ctgggtttcg gcgtgatgat taccttatcc agctatctgg ataaaaacga
     6661 aaatctggtt cagacggcag ttatcacggc aattaccaat accatcatcg ctgtacttgc
     6721 gggctttatg attttcccgt cgctcttcag cttcggcgtc gcgcccaatt ccggcccgac
     6781 tttggtgttc caaagtttac cgattgtgtt ctcacatatg tgggcaggac ctgtgttcgc
     6841 cgtcattttc ttctctctgc tcctgattgc cgcgttgaca acttcgctga cgatttatga
     6901 agtgttgatt acgaccattc aggaaaaaac caaaatccgc cgtaccgccg caatcacgat
     6961 tgtattgtcc gtcatcttcg ttttcggcaa catcccgtct attttgagct atggcccgtg
     7021 gaaagacgtt tccgtgttcg gcaaaaatat tttcgatgcc ttcgactaca tcagcggcaa
     7081 catcttgttt atgctgaccg cgctcggttc cgcattgttt gtcggttttg tgatgaagga
     7141 cgaagcgaag gaagaattgc tttataaagg caaccatacg acggtcaata tttggtttgc
     7201 ctatgtgaaa taccttgtgc cgctggtgat tctgctgatt ttcgtcagca acctgttcta
     7261 atccgcagca atcaatgccg tctgaaggtc atacctcttt cagacggcat tgcattgatg
     7321 cccgccacgc cggaatccga accggcttgc cggcttctgt cggacgcttt ccgggcaggc
     7381 ggcgttccgt ctgcttagac aatcgtcctt taaaacaggt agaatccgcc ccaacgggaa
     7441 acacaccctt cagacggcaa aacccatacc ccaaaccatc aggaatcccc cttatgaaca
     7501 gacaaaaagt catcgccatc gacggtccgg gcgcatcggg caaaggcacg gtcgccgccc
     7561 gcgttgccgc cgcattggga tacgattatc tcgataccgg cgcgctctac cgcctgaccg
     7621 ccctatatgc acaaaaacaa ggcgtggaat ggcacgatga agaaaacgtt tccgcacttg
     7681 ccaaaaaact gcccgccgta ttttcaggca accgcatcct acttgatggc gaagacgttt
     7741 cagacggcat ccggacagaa gccatcggta tgggcgcatc cgcagttgcg caatggccca
     7801 aagtccgcgc cgcactgctg caacgccaac gcgattttct gaccgaaaaa ggactggttg
     7861 ccgacggacg ggacaccgga tcggtcgtct tcccccaagc cgaactcaaa atttttctga
     7921 cagccgaatc taaaatccgt gccgaacgcc gcgccaaaca aatcggcatt ccctgcgaag
     7981 gtttcacatt cgagcgcatc ctgtccgaca tcgagaccag agacgaagca gaccgaaacc
     8041 gcaaggttgc ccccctgaaa caacagcccg atgccctgct tttggatacg agccgcctaa
     8101 ctatagaaga aactgtaaaa aaagtgcttg attggtatcg taaagtttaa attttcaggt
     8161 ataatcgccc gcattgcgct tcagacagca tgaatttcca tgtgccgtct gaaaccttat
     8221 ttacccgcct gccgtgccaa ggactgcaga cacccaccaa cccaacccgc accccttggc
     8281 ggtgtaccga aaagagttat atatgtctat ggaaaatttt gctcagctgt tggaagaaag
     8341 ctttaccctg caagaaatga acccgggtga ggtgattacc gctgaagtag tggcaatcga
     8401 ccaaaacttc gttaccgtaa acgcaggtct gaaatcagaa tccctgatcg atgtagctga
     8461 attcaaaaac gctcaaggcg aaattgaagt taaagtcggc gacttcgtta ccgttaccat
     8521 cgaatccgtc gaaaacggct tcggcgaaac caaactgtcc cgcgaaaaag ccaaacgcgc
     8581 agccgattgg atcgctttgg aagaagccat ggaaaacggc aacatcctgt ccggcatcat
     8641 caacggtaaa gtcaaaggcg gcctgaccgt tatgatcagc agcatccgcg cattcctgcc
     8701 gggttctttg gtcgacgtac gtcccgttaa agacacttcc cattttgaag gcaaagagat
     8761 cgaattcaaa gtgatcaaac tggacaaaaa acgcaacaac gtcgttgttt cccgccgcgc
     8821 cgttttggaa gccactttgg gtgaagaacg caaagccctg ctggaaaacc tgcaagaagg
     8881 ctccgtcatc aaaggcatcg tcaaaaatat caccgactac ggcgcattcg ttgacctggg
     8941 cggcatcgac ggcctgctgc acatcaccga tttggcatgg cgtcgcgtga aacacccgag
     9001 cgaagtcttg gaagtcggtc aggaagttga agccaaagta ttgaaattcg accaagaaaa
     9061 acaacgtgtt tccttgggta tgaaacaact gggcgaagat ccttggagcg gtctgacccg
     9121 ccgttatccg caagccaccc gcctgttcgg caaagtatcc aacctgaccg actacggcgc
     9181 attcgtcgaa atcgaacaag gcatcgaagg tttggtacac gtctccgaaa tggactggac
     9241 caacaaaaac gtacacccga gcaaagtcgt acaactgggt gacgaagtcg aagtcatgat
     9301 tttggaaatc gacgaaggcc gccgccgtat ctctttgggt atgaaacaat gccaagccaa
     9361 tccttgggaa gaatttgccg ccaaccacaa caaaggagac aaaatctccg gtgcggttaa
     9421 atccattacc gatttcggcg tattcgtcgg cctgcccggc ggcatcgacg gtctggttca
     9481 cctgtccgac ctgtcttgga ccgaatccgg cgaagaagcc gtacgcaaat acaaaaaagg
     9541 agaagaagtc gaagccgtcg tattggcaat cgatgtggaa aaagaacgca tctccttggg
     9601 tatcaaacaa ctggaaggcg atcctttcgg caacttcatc agcgtgaacg acaaaggttc
     9661 tttggttaaa ggttccgtga aatctgttga tgccaaaggc gctgttatcg ccctgtctga
     9721 cgaagtagaa ggctacctgc ctgcttccga atttgcagcc gaccgcgttg aagacttgac
     9781 caccaaactg aaagaaggcg atgaagttga agccgtcatc gttaccgttg accgcaaaaa
     9841 ccgcagcatc aaactttccg ttaaagccaa agatgccaaa gaaagccgcg aagcactgaa
     9901 ctccgtcaat gccgccgcca atgcgaatgc cggtaccacc agcttgggcg acctgctgaa
     9961 agccaaactc tccggcgaac aagaataagg ttgcagacat gacaaagtct gagttaatgg
    10021 ttcgcttggc agaagtattt gccgccaaaa acggcacgca tcttctggca aaagacgtag
    10081 agtacagcgt aaaagtcttg gttgacacca tgacccgatc gcttgcccga ggtcaacgca
    10141 tcgaaatccg cggtttcggc agcttcgatt tgaaccatcg tcctgcccgc atcggtcgca
    10201 atcccaaaac cggcgagcgc gtggaagtac ctgaaaaaca tgtaccccac ttcaagcctg
    10261 gtaaagaatt gcgcgagcgg gtcgacttgg ctttaaaaga aaatgccaat taaaccttag
    10321 catcaaaacg ccgctggtac gcggcgtttt ttctttggtt taacttcatc cgctgcttca
    10381 ataccttaag ccaagcaagc aacggattag agcgtggatt ttttaccatt gcttacaatc
    10441 cgctttttaa acaacaaatt gttgatttct attacgaaca ggacaaaatc ctgcttattg
    10501 cactaaaact aagccgtttc aggaatttgc ggcaaattta cagcttttac cgagcctaat
    10561 gctttcgctt tttggtaaaa cgccaatttg tattcaagca aatctaaata acgttttaat
    10621 tcggcaattt gacacttcac attttctatt tgattttcaa acaaggaaag gcgttcttca
    10681 atggtatcgt cgccaatgac ggtacattcc gcaaagcgtt tgatgtcttt taagctcatt
    10741 cccgtatttt tcaagcattg caataagtac aaccattgca aatcgttatc ggtaaaacaa
    10801 cggttaccgt attcatcacg tccgatatta ggcaacaagc cttctttgtc gtaaaagcgt
    10861 aaagtgtggg cggagatgcc tattttttcg gcagctttgg cagtggtata agtcattttc
    10921 cctccctcta aacaaaaaac ggtaaaaaaa cacttgcctt agagtgaact ctaaaatgta
    10981 aactgttgct atgttgctca ggcaaggcta ttttgttaat gaattaagag gaaagacaat
    11041 ggaaatgaaa caaaccgatt caaccatcaa atctcgtgcg gcggtggcat tcgcccctaa
    11101 ccaaccctta caaattgtgg aaatcgacgt ggaaatgccg cgtaaagacg aggtgttaat
    11161 tcgtaacacc cacactggcg tgtgccatac tgatgcattt accttatcag gaagcgatcc
    11221 tgaaggcgta ttccctgtgg tgcttggaca cgaaggtgcg ggtgtggtcg ttgctgtggg
    11281 cgagggtgtg tcaagcgtaa aaccgggtaa tcacgtcatt ccgctttaca ccgcagaatg
    11341 tggcgaatgt gagttttgcc tctcgggtaa aaccaattta tgcgtctcag tgcgtgatac
    11401 acaaggtaaa ggcttaatgc cagacggcac gacgcgtttt tcttatcaag gtcagccgat
    11461 ttatcactat atgggctgtt cgactttcag tgaatattct gttgttgccg aagtttcact
    11521 ggcgaaaatc aacccgaaag ccaaccacga acaagtatgt ttgctcggct gcggcgttac
    11581 cactggtatt ggtgcggtac ataacacggc aaaagtacaa gaaggcgatt ctgttgccgt
    11641 gtttggcttg ggagcgattg gtttggctgt ggtgcaaggt gcgcgtcaag ccaaagccgg
    11701 tcgcattatc gccattgata ccaatcctgc aaaattcgag ttggcaaaac agtgttggtg
    11761 caactgactg tttgagcccg aaagattacg ataaaccaat caaagatgtg ttgttagaca
    11821 tcaacaaatg gggcattgac cacacctttg aatgtatcgg caatgtaaac gtaatgcgtc
    11881 aggcattaga aagtgcacac cgaggttggg gacaatccat catcatcggc gtagcggatg
    11941 caggacaaga aatttcaacg cgtccgttcc aatcggtaac aggccgtgtt tggaaaggct
    12001 cggcatttgg tggtgttaaa ggccgctccg agcttccgaa aatggtggag gattcaatga
    12061 aaggcgatat ccaattagaa ccatttgtga cccatgcaat gacccttgat caaatcaatg
    12121 aagcctttga gttaatgcac gaaggtaaat cgatccgcgc tgttattcat tactaaggtc
    12181 cacgatgaaa ctgattgaac aacatcaaat ttttggtggt tcgcaacaag tttgggcgca
    12241 tcatgcccaa acgcttcaat gcgaaatgaa atttgccgtc tatttgccgg ataatccgga
    12301 aaatcaaccg cttggtgtga tttattggct ttccggcttg acgtgtacgg aacaaaattt
    12361 cattaccaaa tcgagcttcc ggcgttatgc ggcagaacat caagtggttg tggtcgcccc
    12421 cgataccggc cctcgcggag agcaagtgcc gaacggtgcc gcttatgatt taggacaggg
    12481 ggcaggcttt tatttgaatg cgaccgaaca gccttgggcg gcgaattatc aaatgtatga
    12541 ttacattttg aacgagttgc cccgtctgat tgaggagcat tttcctacca acggcaaacg
    12601 ttccattatg gggcattcga tggacggaca cggcgcattg gtattggcac tacggaatcg
    12661 ggaacattat caaagtgttt ctgccttttc gcctatttta tcgccaagcc tcgtaccgtg
    12721 gggagaaaaa gcctttactg cttatttagg gaaagaccgt gaaaaatggc agcaatatga
    12781 tgctaactca ctcattcaac aaggctataa agtgcaaggt atgcgtatcg atcagggctt
    12841 ggaagatgag tttttgccga cacaattgcg taccaaagat tttatcgaaa cctgtcgtgc
    12901 ggcaaatcag ccaatcgatg tgcgtttcca taaaggatac gatcacagct attacttcat
    12961 cgccagtttt attggtgagc atatcgctta tcacgccgca tttttgaagt aagtcaaaga
    13021 gcggtcagtt ttcaaagcag tttggaatag ccggcacgag ggcggtaaga agtgccggca
    13081 taaacgtatg ccgcctgaac cggaaggagc cggccctacg gattaccaaa ccctgccctt
    13141 gatttgatta gtatgatttt taagcttaaa ccctgattga tcaaacatta aaatcgcccg
    13201 tatttcctac ttcactttcg ggcttcgtat tattcgttgc cgaatttgca taagcaacct
    13261 gttcctgttg tgttttcgcc ccctccaagc tgacggtggc gtaacagctt cgcgattcac
    13321 catctgtttc atttgtcctc cttcaatatt aaaacgcagc ctgaaataaa aggcattttg
    13381 gctcatatct gcaccatatt aaaacgccgc ctttgcttat accccctttg tgcgcgtcat
    13441 tattcttttc cacgggaaac gccaagtttg aaggaaatca tttataatac cagcggtaag
    13501 cattttcttt cttagccgca agaagtataa caaggttaaa tatgaataat agagaccaac
    13561 tttttaaagc cccgccgttt gaaaaccaca gcccgctgac ctggtatcag gctgcctcac
    13621 aactgcccaa cttcatccgc gacgacgcac aggcggccgc catcgaacac cttgaccggc
    13681 tttggaccga attgatgatg ttcaaacgca aaagaaaccg ttttttaggc aggagtttgc
    13741 gttccccgca agttcccaaa gggctttatt tctatggcgg agtcggacgc ggcaagagct
    13801 ttctgatgga cgcttttttc ggctgcctcc cttaccgccg caaacgccgc gtccactttc
    13861 atgcctttat ggcggaaatc caccggcggc tgaaagccct gaaaagcgaa agcaacccgt
    13921 tgaaatccgt tgccgccgag attaccaaag aaacccgagt attgtgtttt gacgaatttc
    13981 atgtcagcga tattgcggat gcaatgattt taggccgtct gctggaaaac ctgcttaacg
    14041 agggcgttgt tttggtggcg acttcaaact acgcgccttc cgaactctac ccgcaaggtc
    14101 aaaaccggag cggttttctt cccacaatcg cgctcatcga gtccagtctg accgtcttaa
    14161 acgttgacgg aggggaagac taccggctgc gtaccctccg ccccgccgag attttcttta
    14221 cgcctgccaa tgaagaaaat gaggcaaaac tggcaaaact gttcaaagaa atgacaggca
    14281 ttaccgattt gaaccccggc atcagcacca tccacggccg ggaaattccc cacaaagccg
    14341 agtccggtcg taccatatgg tttgatttcc gcgcactgtg cttcagtccc cgctcacagt
    14401 ccgactatct gtatttggcc gaacattatg aaatggtttt tatttcaggt ttggaacaac
    14461 tctcaccgca agaaaaggcg gaggcgcgac ggctgacttg gctgattgac gtactctacg
    14521 atttccgggt caaactgtgt gccaccggcg cggtagatgt caaccatatc tacacggaag
    14581 gcgattttgc cgaagaattt acccgcaccg ccagccggat ggtcgaaatg cagtccgaag
    14641 tttatttgga acagccgcac ctgaccttat ctcccaaggc ttcaggcgga taagttattt
    14701 ttttggtaga ataccgattt gattcttctt aagtaaaaat aaggatatag catggcgatt
    14761 gaacgtacca tatccatcat caaacccgat gccgtcggca aaaacgttat cggcaaaata
    14821 tacagccgct ttgaggagaa cggtctgaaa atcgttgccg ccaaaatgaa gcagcttacc
    14881 ctcaaagaag cccaagaatt ttatgcggtt cataaagacc gccccttcta tgccggattg
    14941 gttgaattta tgaccggcgg tccggttatg attcaagtac tggaaggcga aaacgccgtt
    15001 ctgaaaaacc gcgaactgat gggggcgacc aatcccaccg aagccgcaga aggcacgata
    15061 cgcgcggact ttgccacttc ggtcagcatt aacgccgtac acggttccga cagtgtggaa
    15121 aatgccgctt tggaaattgc ctactttttc agccaaaccg aaatctgccc ccgttgatac
    15181 aatacaccgc ccaattccta ttcagacggc ataaaatata tccatgccgt ctgaaaactc
    15241 tgttgcaaaa gacttcaaat caaacttgcc tgccctgcaa ttttttattt gaagccttaa
    15301 tttaagaaaa acacaaatac atgaaaacca atctgctcaa ctacgacctt caagggctaa
    15361 cccgacattt tgccgatatg ggtgaaaaac cattccgcgc caaacaggtt atgcgttgga
    15421 tgcaccaatc cggcgcgcaa aattttgacg aaatgaccga tttggcaaaa tcgttgcgcc
    15481 ataaactgaa cgaacaggca agcatcgaaa ttcccaagct gatgatgtcc caagaatctt
    15541 cagacggcac tcgaaaatgg cttttggatg tcggtacggg gaacggcgtg gaaaccgtct
    15601 tcatccccga atcggatcgc ggcacgctct gcatttcctc gcaagtcggc tgcgctttgg
    15661 aatgtacatt ttgttcgacc ggccggcagg gcttcaaccg caatttaacc gctgccgaaa
    15721 tcatcggaca gttgtggtgg gcaaacaaag cgatgggcgt tacaccgaaa aacgagcgtg
    15781 tgatttccaa cgtcgtcatg atgggcatgg gcgagccgat ggcgaacttc gacaatgtcg
    15841 ttaccgcctt gagcatcatg ctggacgacc acggctacgg tttgagccgc cgccgcgtaa
    15901 ccgtttccac ttcgggtatg gttccccaaa tggacaggtt gcgcgatgtc atgccggtgg
    15961 ccttggcggt ttccctccac gcttccaatg acgaagtccg caaccaaatc gtaccgttga
    16021 acaaaaaata tcccttgaaa gaattgatgg ccgcatgcca acgctatctg gtcaaagcgc
    16081 ccagggattt catcactttc gagtatgtga tgctggacgg tgtcaacgat aaggcacaac
    16141 atgcatacga acttattgaa ctggtcaaag atgttccctg caaattcaat ttaatcccgt
    16201 ttaatccttt ccctaattcg ggatatgaac gttcaagtaa cgaaaacatc cgtattttca
    16261 gagatatcct gcaacaagcc gaatttgtcg ttaccgttcg aaaaactcgt ggcgacgata
    16321 tcgatgccgc ctgcggacag ttggcaggtc aggttcagga taaaacgcgc cgccaacaaa
    16381 aatggcagca gattttaatc ggacaacagg ggtaattatg ccttttaagc catccaaacg
    16441 aatctcttta ttacttgttc ttgccttggg cgcgtgcagc acttcctacc gcccctcgcg
    16501 ggcagaaaaa gccaatcagg tttccaatat caaaacccag ttggcgatgg aatatatgcg
    16561 cggtcaggac taccgtcagg caacggcaag tattgaagat gccttgaaat cgaaccctaa
    16621 aaacgagctt gcctggctgg tccgtgccga aatctatcaa tacctgaaag ttaacgacaa
    16681 ggcgcaggaa agtttccggc aagccctctc catcaaaccc gacagtgccg aaatcaacaa
    16741 caactacggc tggttcctgt gcggcaggct caaccgccct gccgaatcta tggcatattt
    16801 cgacaaagcc ctggccgacc ccacctaccc gaccccttat attgccaacc tgaataaagg
    16861 tatatgcagc gcaaaacagg ggcaattcgg attggcggaa gcctatttga aacgttccct
    16921 cgccgcccag ccgcagttcc cacccgcatt taaagaactg gcgcgcacca aaatgctggc
    16981 cgggcagttg ggcgatgccg attactactt taaaaaatac caaagcaggg tagaagtcct
    17041 tcaggccgat gatttgctgc taggctggaa aattgccaaa gccctcggca acgtgcaggc
    17101 ggcatacgaa tatgaagcac aattgcaggc aaatttcccc tactcggaag aattgcaaac
    17161 cgtcctcacc ggtcaataaa cagattcaaa ccatatgaac acactccaac gccgcaagac
    17221 gcatcaagtc ctcatcgatc atattaccgt cggttcagaa gcacccgtcg ttatccaatc
    17281 tatgaccaac accgacactg ccgatgcaaa agccaccgca ttgcagatta aggaattgag
    17341 cgatgccggc tcggaaatgg tgcggattac cgtcaacagc cccgaagccg cgtccaaagt
    17401 tgccgaaatc cgccgccgct tggacgatat gggctatgcc acaccactga ttggcgattt
    17461 ccattttaac ggcgaacgcc tgttggcgga atttcccgaa tgcggcaaag cattgtccaa
    17521 ataccgcatc aatcccggca atgtcggcaa aggcgtaaaa ggcgatgaaa aatttgcctt
    17581 tatgattcgg actgctgctg aaaacgataa agccgtccgt atcggcgtaa actggggttc
    17641 tttggatcag agcctcgcca aacgtatgat ggatgccaac ctcgtttctt ccgcgccgaa
    17701 accgcccgaa gaagtgatga aggaagcact gattgtctcc gctttggaat ctgccgaaaa
    17761 agccgttcta ttgggactgc ccgaagacaa aatcatcctg tcgtgcaaag tcagcgcggt
    17821 tcatgatttg attcaggttt accgcgaact gggcagccgt tgcgtctatc cgctgcattt
    17881 gggtttgacc gaagccggta tgggcagcaa aggcattgtc gcatcaacgg cggcattatc
    17941 tgttttgctt caagaaggaa ttggcgacac catccgcatt tcactcactc cggaaccggg
    18001 cagcccgcgt actcaggagg tcgtcgtcgg gcaagagatt ttacagacta tgggtttgcg
    18061 ctcgtttacg ccgatggtta ccgcctgccc gggatgcggg cgtaccacca gtaccgtatt
    18121 tcaagagctg gcacaagatg ttcaaaatta cctgcgccaa aaaatgtcta tatggcgtac
    18181 cctttatccc ggggttgaat ccctgaacgt tgccgtaatg ggctgcgttg tcaatggccc
    18241 cggagaaagc aaattggccg acatcggaat cagccttccc ggtacggggg aaacacccgt
    18301 cgcacctgtt tatgtagatg gggagcgcaa agtcacactg aaaggtaata atattgcaag
    18361 cgaattccta gctattgtag aggaatatgt taaaaccaat tatggcaaaa acagttctaa
    18421 acgcaataaa ggaaaagtca tcccgataca gtctctataa aacaaatgcc gtctgaacgt
    18481 ttcagacggc atttgtttct ctcagatttc ttattaaagc cgcaaagaag cgcggttttc
    18541 caaaacttgg tcgatcaaac catattcttt tgcttcttcg gcagacatga aattatcacg
    18601 gtcggtgtcg cgctccaaat ctgccaaatc gcggccgcaa tgtttcgcca tcaggcggtt
    18661 gagtttttct ttgattttca acaactcgcg tgcgtggatt tcaatgtcgg atgcctgacc
    18721 gcccaagccg ccgctgatta aaggctggtg aatcataatc cggctgttgg gcagggcgaa
    18781 acgtttgcct ttctcgcctg ccgacaataa gaacgcgccc atacttgccg cctgccccaa
    18841 gcacaaagtc gatacatcgg gcttgatgaa attcatcgtg tcgtaaatcg acataccggc
    18901 cgttaccgag ccgccggggg aattgatgta gaagaaaata tccttatccg gattctcact
    18961 ttccaaaaac aacagttggg cgaccaccag attagcagac tcatcggtta ccgggccaac
    19021 cagaaatacg atgcgctctt tcaaaagccg ggaatagata tcgaatgcac gctcaccgcg
    19081 accgctctgc tcgataacgg tagggacaag atggttatca aaagacattt cgtctccttt
    19141 catgatggaa aagcaccaaa gcgggcttta aaaacggctt cggtgctttc aaaaactgcc
    19201 ttcagacagc attttcagaa caatcaggct tgcgcgccca tcacttcgtc aaaagacaaa
    19261 gcttttttgt ttactttggc tttgcccaaa acgaaatcaa caacgttgct ttctactgcc
    19321 aaagaagtcg gggcttgcag gcgggaagta tctgcgtagt accagtcaat cacttcttga
    19381 ggatcttcgt agctttctgc gaagttggcg acaacggctt tgatttgctc ttcagtcggt
    19441 tccagtttgt tttcgtcaac cagtttggcc aaaatcagac ccaaagatac gcgacgttcg
    19501 gcttgttctt tgaacatatc caaaggcaaa tccaagttcg cggcatcggt cataccttgg
    19561 ttaacgaagt tttgtttcat ttcgtttgcc aggcgggcgg cttcttcatt gaccaaagca
    19621 accggaactt tcaactctac ggctttaatc agcgcgttca ttacggattc tttggtttgt
    19681 tcgttcacgc ggcgttccac ttcgcggctt acgtttttct tcacttcttc acgcattttg
    19741 gcaacatcgc catccgcaat acccaaggct tttgcaaaat ctgcatcgac ttcaggcaga
    19801 gtgggctcgg aaacattgtt cagcgtaatg gtgaacacgg cagttttacc ggcaacatct
    19861 ttgccgtggt attcttcagg gaagttgacg gtaacgtctt tactttcgcc cgctttcatg
    19921 ccgactacgc cggcttcaaa ttcaggcagc atttgacctg cgcccaatac gaaggcgtag
    19981 tttttggatg cgccgccggc aaaaggttcg ccgtcgattt tgccttcaaa gtcgatgatg
    20041 acgcggtcgc cgtttcgggc ttcgcggtca acatggttga agcgggtacg ttgtttacgc
    20101 aggatttcta cagtttggtc gacttcggca tcgccgacgg aagcggttac tttttcgacc
    20161 tcttgtgcag acaaatcgcc gataacgact tcggggaaca cttcaaaaat ggcggcaact
    20221 ttgaaagact ctttatcgtc ttgttcttca acgccttcaa aacgtggata gcctgccact
    20281 ttcaactctt gggcaacggc aacatcgtag aagcggcgtt gcaccagctc gttgatcacg
    20341 tcgttttgcg cgctcgcacc gtacatttgg gcaatcattt ttaaaggtgc tttacccgga
    20401 cggaaaccgt cgatttttgc acggcgttgg gtttgtttca gttttttatc ggtttctgcg
    20461 ttgatttcgg accaaggcag ggacaacact actttgcgtt ccagattttc taaaatttca
    20521 acagttacgc tcatcataag cccttaaatt tgttgtgttg ataaaatgat aaaccttctt
    20581 ccctgcatgg ggaagcaaac agcgcaacgg tacgatattt gaaccgcatt gccgcaaagg
    20641 ggaaatttta gctggcaagt atatcacaat gtttcgcctg aaacataata tgccgagacc
    20701 tttgcaaaat tcccctaaaa tcccctaaat tcccaccaag gcatttaggg gatttcccat
    20761 ga
//
LOCUS       DAXDXW010000032        20759 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXDXW010000032 DAXDXW010000000
VERSION     DAXDXW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20759
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.033"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(49..720)
                     /locus_tag="ACFB64_001036"
                     /pseudo
     CDS             complement(49..720)
                     /locus_tag="ACFB64_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            980..2452
                     /gene="pyk"
                     /locus_tag="ACFB64_001037"
     CDS             980..2452
                     /gene="pyk"
                     /locus_tag="ACFB64_001037"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC1209408.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HILKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQTEYPEAVSTNLAIAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            2686..4038
                     /locus_tag="ACFB64_001038"
                     /pseudo
     CDS             2686..4038
                     /locus_tag="ACFB64_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
     gene            complement(4121..4543)
                     /locus_tag="ACFB64_001039"
     CDS             complement(4121..4543)
                     /locus_tag="ACFB64_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209409.1"
                     /translation="MAGCLTDLENCLEKYLEQFGPVSESIEACTAKLQEQPSFFNRLM
                     KANDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSIHNRKADYAEKGKERIAL
                     FPRGLNGITKLPAAVLLPERPYHFDMKEVLYIFSRIPR"
     gene            complement(4726..4875)
                     /locus_tag="ACFB64_001040"
     CDS             complement(4726..4875)
                     /locus_tag="ACFB64_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209410.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVSYADVSTHRTAHKAS
                     PYVPS"
     gene            complement(4893..5090)
                     /locus_tag="ACFB64_001041"
     CDS             complement(4893..5090)
                     /locus_tag="ACFB64_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209411.1"
                     /translation="MNTVPKSRIPVKPLPEKTTAEAKVEKWRQLGADHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            complement(5100..6116)
                     /locus_tag="ACFB64_001042"
     CDS             complement(5100..6116)
                     /locus_tag="ACFB64_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC1209412.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQDIRGSIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAEANSPLLYGETSLADIVQQKTGGNVEFKDGVLTAAVRFLPAKDARTAFIDNTV
                     GMATQTLSAALLPYGVKSIVMIDGKAVTKEDAVRVLSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDVERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            complement(6215..7429)
                     /gene="gltS"
                     /locus_tag="ACFB64_001043"
     CDS             complement(6215..7429)
                     /gene="gltS"
                     /locus_tag="ACFB64_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC1209413.1"
                     /translation="MEWAFNSYYTLIAATLVLLVGKVLVKKIKILRDFNIPEPVAGGL
                     IAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENTKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGSVTVILAVQTAVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            complement(7682..7963)
                     /locus_tag="ACFB64_001044"
     CDS             complement(7682..7963)
                     /locus_tag="ACFB64_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209414.1"
                     /translation="MKQIKASDFTDEHFGLPTILDILSELEKPQPRTDSRPQTTTQRA
                     CPNQFGDGGCVCEVEAVSPTAGMYSDRCSGGFCPRFFCYFKYVYSHDAV"
     gene            complement(7982..9106)
                     /locus_tag="ACFB64_001045"
     CDS             complement(7982..9106)
                     /locus_tag="ACFB64_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC1209415.1"
                     /translation="MSNNQPNSQIYEDYWAFTNAFTNYNDQKFCTALNICLDFIDANQ
                     DQPYSNELYEALQQNIAQDSVMASQRTSKAMNLASVRKAINQFVKMGFIEPFLSSYTP
                     LSRDYVQARTNRKRQTLLSKIVYTHSGFQRSVTENSNIRQINFLIKTLVEHPQGKLNK
                     KEIAAMMLVDLKTFQQDYLTETELNDYFQQGIESGFIERKYNQISYLWNLLDKLDDLK
                     RVGDDLYFAEDAQRIFGNLDEITVRKRDPYLHRLYKNQLQEESEEHYGNVKCMLEKLA
                     YPVLIASHIKPFILSDDTEAYDPNNGLLLSRTLDSLFDLKYISFDDEGNMVKSKRLSD
                     DVWRRWCDVKLDNNLLNDKRKSYLAYHRELMLQEDQEFHI"
     gene            complement(9169..10176)
                     /locus_tag="ACFB64_001046"
     CDS             complement(9169..10176)
                     /locus_tag="ACFB64_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFC1209416.1"
                     /translation="MNKKYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYSEK
                     FYFHDECQQLDLYRKNIGKLNNQYKRALAFTLMRRAMIRKMPYSRFTIPWEKVKQLRD
                     EEYSYAKYGRRRAYHNESFQSHFLQNLDDYNQAVFNNGWRHHAFNQDIFDLLPNIQAD
                     AVYLDPPYTGTMNNYFGFYGLLDSYITSSIPKPFANHFMDKKQAVELFKRVINHLKPF
                     KYWLLSYNNASYPNRDELEEMLGKNGRNVQILETPHVYKVTGKEKKQSHKEILFLVEN
                     T"
     gene            complement(10169..11170)
                     /locus_tag="ACFB64_001047"
     CDS             complement(10169..11170)
                     /locus_tag="ACFB64_001047"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1209417.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPRHQLLQKDLTTLTEQDLTNCLNGQSVDLVIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKPAVCITGDMRKVFHY
                     EQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNAVPPLLAKAIAESILKMSENE"
     gene            complement(11201..13474)
                     /locus_tag="ACFB64_001048"
     CDS             complement(11201..13474)
                     /locus_tag="ACFB64_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFC1209418.1"
                     /translation="MNITRILSQELSATTVQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRQLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQPQDVEAAAQGYLNENIPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHTQVVEGKETEGEKFSDYFDRREPVRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     GMPESSLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSRLAKSLDAVVEDCVNAVGVDANTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGITAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGAFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRD
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPTNSAMADAFAKLKR"
     gene            13589..14605
                     /gene="galE"
                     /locus_tag="ACFB64_001049"
     CDS             13589..14605
                     /gene="galE"
                     /locus_tag="ACFB64_001049"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC1209419.1"
                     /translation="MTVLITGGTGFIGSHTAVSLVQSGYDAVILDNLCNSSAAVLPRL
                     RQITGRNIPFYQGDIRDCQILRQIFSEHEIESVIHFAGLKAVGESVAEPTKYYGNNVY
                     GSLVLAEEMARAGVLKIVFSSSATVYGDAEKVPYTEDMRPGDTANPYGASKAMVERML
                     TDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGVPNNLLPYICQVASGRLPQLSVF
                     GGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGVAGVHLFNLGSGRAYSVLEIIRAF
                     EAASGLHIPYRIQPRRAGDLACSYADPSHTKQQTGWETKRGLQQMMEDSWRWVSRNPG
                     RYGD"
     gene            14606..15646
                     /gene="rfbB"
                     /locus_tag="ACFB64_001050"
     CDS             14606..15646
                     /gene="rfbB"
                     /locus_tag="ACFB64_001050"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC1209420.1"
                     /translation="MQTAGKKNILVTGGAGFIGSAVVRHIIQNTRDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQYRPDAVMHLAAESHVDRAIGSAGEF
                     IRTNIVGTFDLLEAARAYWQQMPSEKREAFRFHHISTDEVYGDLHGTDDLFTETTPYA
                     PSSPYSASKAAADHLVRAWQRTYRLPSIVSNCSNNYGPRQFPEKLIPLMILNALSGKP
                     LPVYGDGAQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKTNLEVIKTICALLE
                     ELAPEKPAGVARYEDLITFVQDRPGHDARYAVDAAKIRRDLGWLPLETFESGLRKTVQ
                     WYLDNKTRRQNA"
     gene            15751..16617
                     /gene="rfbA"
                     /locus_tag="ACFB64_001051"
     CDS             15751..16617
                     /gene="rfbA"
                     /locus_tag="ACFB64_001051"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC1209421.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNAAFQRLLGDGSDFGIRLQYAVQPSPDGLAQAFIIGEEFIGNGNV
                     CLILGDNIFYGQSFTQTLKQAAAKTHGATVFGYRVKDPERFGVVEFDENFNALSIEEK
                     PQQPKSDWAVTGLYFHDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTQESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLTKKDVETRAKPLEKT
                     AYGQYLLRLIGK"
     gene            16756..17313
                     /gene="rfbC"
                     /locus_tag="ACFB64_001052"
     CDS             16756..17313
                     /gene="rfbC"
                     /locus_tag="ACFB64_001052"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC1209422.1"
                     /translation="MDIIDTALPDVKLLKPQVFTDGRGFFMETFRDGWFKENIADRTF
                     VQENHSNSSKGVLRGLHYQTENTQGKLVRIVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNRYQLWIPEGFAHGFCVLGDAAEVVYKCTDYYNPETEQVLIWNDPAIGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLTLSR"
     gene            complement(17357..19375)
                     /locus_tag="ACFB64_001053"
     CDS             complement(17357..19375)
                     /locus_tag="ACFB64_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC1209423.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDDRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVAVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAALQATLMT
                     TIASGIFAHNLEWAYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNADRIGTLFAAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(19570..20691)
                     /gene="dnaJ"
                     /locus_tag="ACFB64_001054"
     CDS             complement(19570..20691)
                     /gene="dnaJ"
                     /locus_tag="ACFB64_001054"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC1209424.1"
                     /translation="MSNQDFYATLGVARAATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGAS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCRGAGKHIKEPCVKCRGVGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
BASE COUNT         5178 a         5668 c         5018 g         4895 t
ORIGIN      
        1 ccgaccacgc aagccgccga acaaaaaata aggggctgtc ctagataact aggataaact
       61 cgattttact aattgtttta aaatggaaca agaactttta tctcactgtt gttaaaacgc
      121 cattcgcact cctttaaata cagctcaaaa tgcgctttgg gaatgccgtt aaacttgcgt
      181 aaatgacgtt ttgcctgatt ccaaaagttc tcaattccat taatatggtt ttgtcgttcg
      241 gcaaaatgtg tgctgtgatt gatacgaaaa cgaagtttca gcgaagctaa aatggctaaa
      301 ttcgctcaca tctaatacat catagctacg ataacaatcc gtataaacaa tactgtcagg
      361 tttcacttgt tcacggataa taggaaataa agtagcggtt tgagtattcg gtactgtaac
      421 cgtataaacc ttaccatttc gcttcaaaag accgaatacg gcgactttac cggcagcacc
      481 gcgaccgcgt ttgcctttgc gttgtccgcc aaaataactt tcatctgctt ctacttcgcc
      541 atcaaacatt tccaaatgcg gactgttttg ataaataagt aatcgtaaac gatgaaaata
      601 ataggctgcg gtatttttat taacgcctac taactctgct gccgttcttg cagttacacc
      661 tgcgacaaac agttcaatga gtttattttg tttataccgg cttagacgac tttttctcat
      721 agggataatt ctaacttaat ttgaatttcc ctagttatct aggacagccc caaaataaat
      781 ttagcctaaa tcataaaacc gtgtaatttt gcaccaaaaa aggcggtttg caggtaaaat
      841 atcaaaccat taatttaaat catattttta cagaatattc cgccgcgttc accaaatgga
      901 catcaaaccg gttccaaatt tctgtaattt tgcaacaaaa taccgcaaaa cacccgattg
      961 agaccgaaag gactttcata tgaaccagac aagccgcgat ctgacccgca tcagccacaa
     1021 caccaaaatc gtcgccaccc ttgggccggg cagcaacaac gtcgaactgt tggaagacat
     1081 gatccgcgtc ggcggcctga acgtcgtccg cttcaacttc agccacggca cgcccgaatt
     1141 ccatcaggaa aacgcccgca tcgtgcgcga ggcggcaaaa cgcgccggac aggaaatcgc
     1201 catcattgcc gatctgcagg gcccgaaaat ccgcgtgggc aaaatcgccg gcggcggcat
     1261 cgaattgaac aaaggcgaaa cattggtact cgatgccgcg ctcgaaggcg aaggcacgcg
     1321 cgaggcggtc ggtttggact accgcgacct gcccgacgac gttgccgcag gcgatgtctt
     1381 gtggctggac gacggcctgc tgaccctgac cgtggaatcc gtcgaaggca gcaggattat
     1441 cacaagggtg gaaaacagcc acatcctgaa aagcaacaaa ggcatcaaca aacgcggcgg
     1501 cggtttgtcc gcaggcgcgt tgaccgaaaa agacttccgc gacctgaaaa ccgcgattgc
     1561 catcggttgc gactatctcg ccatcagctt tgtgaaatcc gccgaagacc tgcacatcgc
     1621 gcgcgccaaa gtcgaagagg aaatgaaggg cagcactgcc gtccgccccg gtttggtttc
     1681 caaaatcgaa cgcgtagaag cgattgaaaa ccttgatgaa atcatccttg ccggcgacgg
     1741 cattatggtt gcgcgcggcg acttggcggt cgaagtcgga cacgccgccg tccccgccct
     1801 gcaaaaacgg atgatccgcc gcgcccgcga gttgcgccgc ttcagcatta cggcgacgca
     1861 aatgatggaa tcgatgatta ccaaccccgt gccgacccgc gcggaagtca gcgatgtggc
     1921 aaacgcggta ttggacggca ccgatgcggt gatgtgttcc gccgaaaccg ccgtcggcgc
     1981 gtatccgttt gaaaccgtca gccaaatggc gattatctgc gcggctgcgg aaaaagaaca
     2041 ggattcgctc aacggcgttg ccgaacagac agagtatccc gaagccgtca gcaccaactt
     2101 agcgattgcc ggcggcgcgg tcagcgtggc gcgcgcggtt cacgccaaag ccatcgtcgc
     2161 cctgaccgaa agcggttcga ccgcctttga aatcagccgc cacaatatta ccctgccgat
     2221 tttcgcgctg accccgagcg tttccgccca acgccgtatg gcgatgtacc ggggcgtgcg
     2281 cccgctgatt ttggcaacca gcaccgacca cgacacggcg ttgaacgaag tcgaaacgat
     2341 gctggtggaa cacaacatcc tgcattccgg cgaccagtac atcatcacca gcggctcgca
     2401 aatgcgcgaa tccggttcga ccaatacgct ggaagtgctg cgcgtcaaat aatccgccct
     2461 gagtggaaaa tgccgtctaa agccgatact gtcgggcttc agacggcatt ttatttgcat
     2521 tccgtcaggt aaaaccgaaa gaattgtgca aatccggcaa catcggataa aatcgagtgc
     2581 ctatactaaa gcgaaacaag gcatttccga ctgccttttt tatttgtcca ccgtccgcct
     2641 ttttacggaa accgaaatga cccctttcac actgaaaaaa ccgtcctgct gctcggcact
     2701 gcctttgccg ccgcatctgt ccacgcatcc ggctaccact tcggcacaca gtcggtcaac
     2761 gcgcaaagca cggcaaatgc cgccgacgcg tcgaccatct tctacaatcc cgccggcctg
     2821 accaaactcg acagcagcca gatttccgtc aacgccaaca tcgtgctgcc cagcattcat
     2881 tatgaagcag attccgccac cgactttacc gggcttcccg tccaaggttc taaaaacggc
     2941 aaaatcacca aaaccacggt cgcaccccac atttacggcg catacaaagt caacgacaat
     3001 ctgaccgtgg gcttgggcgt gtacgtcccc ttcggctctg ccaccgaata cgaaaaagat
     3061 tccgtgttgc gccacaacat caacaaactc ggtctgacca gcatcgccgt cgaacctgtc
     3121 gccgcgtgga aactcaacga acgccattcc ttcggcgcag gcatcatcgc ccaacataat
     3181 tccgccgaac tgcgcaaata tgccgactga ggaatcccaa aaaaagcgca aatgctgcaa
     3241 gcaacacctt ctaatcctac tgccgctgct caaatcaagg ccgacggaca cgccgatgtc
     3301 aaaggcagcg attggggcgt cggctaccaa ctggcgtgga tgtgggacat caacgaccgc
     3361 gcgcgcgtgg gcgtgaacta ccgttccaaa gtttcacaca cgctcaaagg cgatgccgaa
     3421 tgggcggcag acggcgcggc ggcgaaacaa cagtggaatg acaatatgct cacaccgctc
     3481 ggttacacgg cgaatgaaaa agcccgcgtc aaaatcgtaa cgcctgagtc tttgtccgta
     3541 cacggcatgt acaaagtgtc cgacaaagcc gacctgttcg gcgacgtaac ttggacgcgc
     3601 cacagccgct tcaataaggc ggaactgttt tttgaaaaag aaaaaaatat tgctaatggc
     3661 aaaaaatccg accgcaccac catcaccccc aactggcgca acacctacaa agtcggcttg
     3721 ggcggttctt atcaaatcag cgaaccgctg caactgcgcg tcggcatcgc ttttgacaaa
     3781 ccgcctgtcc gcaacgccga ctaccgcatg aacagcctgc ccgacggcaa ccgcatctgg
     3841 ttctccgccg gcatgaaata ccatatcggc aaaaaccacg tcgtcgatgc cgcctacacc
     3901 cacatccaca tcaacgacac cagctaccgc acggcgaagg caagcggcaa cgatgtggac
     3961 agcaaaggtg cgtcttgcgc acgtttcaaa aaccacgccg acatcatcga cctgcaatac
     4021 acctacaaat tcaaataaac gttacaccgt ttgaatataa aaatgccgtc tgaaacagat
     4081 ttccgtttca gacggcattt ttatccggca gccgatacgc ctatctcggt attcgggaga
     4141 atatatacag aacctccttc atatcaaaat ggtagggacg ctcgggcaac aggacggcgg
     4201 caggcagctt ggtgatgccg ttcaaaccgc gcgggaacag cgcaatccgc tctttgccct
     4261 tttccgcata atctgctttg cggttgtgga tggaaactac ttcgcgatgg cggtaaagca
     4321 gcgtgttcat ttcgatatag gcttcgttgt ggatggctgc cgactgtttc tgcaacacgt
     4381 caatctgcct gttgagcttg tcgttggctt tcatcagcct gttgaagaac gacggttgct
     4441 cctgcagttt ggcggtacag gcttcgatac tttccgatac cggcccgaac tgttcgaggt
     4501 atttttccag acagttttcc aagtcggtca ggcagccggc catcttgcgg gaaaattctt
     4561 cttcaggctt tccctgccga atggacaata tagcggatta acaaaaatca ggacaaggcg
     4621 acaaagccgc agacagtaca aatagtacgg caaggcaagg cgaggcaacg ccgtaccggt
     4681 ttttgttaat ccactatatt tgggaggggc gtaacccctt ccgaatcagg atggcacata
     4741 gggcgacgct ttatgtgccg tcctgtgtgt tgaaacatca gcataggaaa cacaggaaaa
     4801 gccggctgtt ttgcgcctgc gtaccgtatt ggtcaaaatg ccgtctgaaa ccgacaacag
     4861 ggtttcagac ggcatttccg tcaattttcc ctttatctgc cgaacaggtt ggcgatattt
     4921 tccatctgtt cttcggtaaa accgttttcg cccaatctgg cggcaactgc ccattcgccc
     4981 gacaaaccgt ggtccgcacc gagctgccgc catttttcga ctttggcttc ggctgtggtt
     5041 ttttcgggca gcggtttgac gggaatcctg cttttgggga cggtattcat cggtttgccc
     5101 taatcgatgg aatagccgcg aagatactgt atccgttcgc gcgtcatccg cgtgtcgcat
     5161 tggagcttga ggtattcggc gtattcctgc cggtctgcct gcgcggcggc ttgtcggcag
     5221 ttgctgattt tttcctgcgc ccacttgcgc tgttcgccga ccaactcttt ttgcacgtcg
     5281 gtatcgagtc ctccccaaag tttggtaatt tcggattccg cacgttggtt ttgtacgcgc
     5341 gcctcttcca cttcgccccg tgatacggta acggtatcgg cacgctcgac gtcgtcggga
     5401 tgcaggattt cgggttcggg cgcgccttct gcggcttggg gtacgcccgc atcgccgccg
     5461 gcggcattgt gttccaaaat gtcttcgggg gtgggtttgg acggttcttc ttcacgggct
     5521 ttgccgctca aaaccctgac cgcgtcttct tttgtcaccg ccttgccgtc tatcatcacg
     5581 atgctcttca cgccgtaagg cagcaacgcg gcagacagcg tttgcgtcgc cataccgacc
     5641 gtgttgtcga taaatgccgt ccgagcgtct ttggcgggca ggaagcggac ggctgccgtc
     5701 aatacgccgt ctttaaactc gacattgccg cccgtcttct gctgcacgat gtctgccaaa
     5761 gacgtttccc catacagcag ggggctgttt gcctcggcat cggcaagcgt ttcagacggc
     5821 acggtaatgt tcaaatcggc gatacagaac gtgcgcccgc cttcctgcgt ttccgaagcg
     5881 tgttccaaag aaaacgccaa accgtaggcg gcggcgataa ttttgtcggc atcgacaaac
     5941 tgcctgccgt cttcgcgcgc gaaagaacgc gcttcctgcg tgagcgtttc ctgaatactg
     6001 ccgcgtatgt cctgcaacac ggcggggttg gcgcattcca acgccttggg cggttcttcc
     6061 ctgccgcacg ctgcaagcag cagggcaaac ggcagcgcaa tgagtttccg atacatgatg
     6121 ttccttccaa acggtgcaga ccgatggcgg ctatcatagc aaaagcacct gcaggcggta
     6181 aaccttgcag gcgctttgtc ggaaaggcgg aaaatcagcc tttaaagaaa ttcacaaaac
     6241 cggtgagaat cgcggcatta atcaaatcga caaagaacgc gccgaccata ggcacaatca
     6301 aaaacgcctt atgcgacgcg ccgaaagtat gcgtgacgga ctgcatattt gccaccgccg
     6361 tcggcgttgc gcccaaaccg aaaccgcagt ggccggcagc caatactgcg gcatcatagt
     6421 cgcgccccat aaagacatag gtaacaaaag tcgcgtacaa aaccatcact gcggtttgta
     6481 ctgcaagaat cacggttaca gaccccgcca aaccggtcag ctcccacagt ttcaaattca
     6541 gcaacgccat tgccaagaaa agcgaaagcg aagcattgcc gaacacatcg atggcacggt
     6601 cgaacatatt gaccttgaat gcggcggtaa ggatgttgcg gataaccacg ccgccaaaca
     6661 gacaccacac gaatttgggc aggtcgaaca ggtattcttt gtcgaagccg tccataatct
     6721 cggcaaacgc cagacacgcg gcaaacatgg caagcgtttc aacggcagat tccgccgtaa
     6781 tcaggcgggt gcgttttgcc tgctcgaaca cgtcgtccgc gttgtcgtcc tgatcctgtt
     6841 ttgtgttttc aaccggtttg cggcccattt tgttgatcag gcggcgcgca accggcccgc
     6901 cgatcaggcc gccgaacacc agcccgaaag tagccgaagc aatacccaaa ccggttgcgc
     6961 cgaccaagcc gtattgcgtt tcaaaattag gtccccacgc acctgacgtg ccgtgtccgc
     7021 ccgtcagcga caccgaaccg gtaatcagac cgatgagcgg gtccaaaccc aaagccgtag
     7081 ccagtccgac cccgacaaag ttttgcacca agataaatcc gcccacaatc gcggtaaaaa
     7141 ccaccagcgg caaaccgccc gccttcaaac gggaaaaatc cgcgctcaag ccgatggacg
     7201 tgaagaaaat cagcataaac gcattttgca gcggtttctc aaatttgaag ctcacgccgt
     7261 acgcctcgtg cagcgcaaac aggataatcg cggcaatcag cccgcccgcc acgggttcgg
     7321 ggatgttaaa atcacgcaag attttgattt tcttaaccaa aaccttgccg accaacaaaa
     7381 ccaaagtggc ggcaatcaag gtgtaataac tgttaaacgc ccattccata tcgtttgacc
     7441 tcctaaaatg tttttcttta tatccgcccg aaggggacgc aagcgcgaag atattagggg
     7501 aatagttaag gcttgtcaaa gaatcgccgt tttttatagt ggattaaatt taaaccagta
     7561 catcgttgac tcgccttgcc gtactatctg tactgtctgt ggcttcgtcg ccttgtcctg
     7621 atttaaattt aatccactat acaactattt tttacaaaca aaatgccgtc cgaaactccg
     7681 ttcagacggc atcatgggaa tacacatatt tgaaataaca gaaaaagcgg gggcaaaacc
     7741 cgccgctgca tctgtcgctg tacatccctg ctgtcggact taccgcttca acttcgcaaa
     7801 cgcatccgcc atcgccgaat tggttgggac acgctcgttg cgttgtggtt tgcggccggc
     7861 tgtccgttcg cggctgcggt ttttccagct cggacagaat gtccagaatg gtcggcagtc
     7921 cgaagtgttc gtcggtgaag tcggacgctt tgatttgctt cacgcgctcg cggttaactg
     7981 cctaaatatg aaattcttgg tcttcttgta gcataagttc tcgatgatat gccaagtaag
     8041 atttacgttt gtcgttgagc agattgttat ctagcttcac atcacaccaa cgtcgccaaa
     8101 catcatctga taaccgctta gatttcacca tatttccttc atcatcaaac gaaatatatt
     8161 tcaaatcaaa taggctatcc aaggtacgac ttaatagcaa gccgttattt gggtcgtatg
     8221 cttcggtatc atccgacagg atgaaaggct taatatgact ggcaatcaat acaggatagg
     8281 caagtttttc cagcatacat ttcacattac cgtaatgttc ttcactttct tcttgaagtt
     8341 ggtttttgta taggcgatgt aaataagggt cgcgtttcct tacagtaatt tcatccaaat
     8401 taccaaaaat acgttgggca tcctcagcaa aatataagtc atcacctact cgttttaagt
     8461 cgtctaattt gtctaatagg ttccataaat aacttatttg attgtatttt cgttcaataa
     8521 atcctgattc tattccctgt tgaaaataat cattcaattc tgtttcggtt agataatctt
     8581 gttgaaaggt ttttaagtct actaacatca tggcggcaat ttctttttta tttagcttgc
     8641 cttgtggatg ctcgaccagc gttttaatca ggaaatttat ttgtcgaata ttagagtttt
     8701 cagttactga acgctgaaag ccagagtgcg tatatacgat tttggaaagt agggtttgtc
     8761 gttttcggtt agtacgagct tgtacataat ctcgtgataa aggtgtatag cttgataaaa
     8821 acggttctat aaatcccatt tttacaaatt gattaatagc tttacgaacc gaggccaaat
     8881 tcatggcctt gcttgttctc tgcgaagcca taacggaatc ttgggcaatg ttttgctgca
     8941 atgcttcata tagttcgttg ctgtatggct ggtcttgatt cgcatcaata aagtcaagac
     9001 aaatattgag tgcagtacaa aatttttgat cgttataatt tgtaaaggca ttggtaaacg
     9061 cccaataatc ttcataaatt tggctgtttg gctgattatt actcatctct gtctccaaga
     9121 atattcgata ccaatattct gtttttgtat ttaagtaata tctatcattc atgtattttc
     9181 tactaaaaac agaatttcct tatggctttg cttcttttct tttcctgtaa ccttataaac
     9241 gtgaggcgtt tccaagattt gtacatttcg cccattttta cctaacattt cttctaattc
     9301 atctcggttt ggatatgaag cattgttgta gcttaacagc caatatttaa atggtttcaa
     9361 atgattgatt acccttttaa ataattcgac agcctgcttt ttatccatga aatgattggc
     9421 aaatggtttg ggtatggatg atgtaatgta actgtccaat aatccgtaaa agccaaaata
     9481 attgttcatc gtgcctgtgt aaggtgggtc taaatagacg gcatcagctt gtatattagg
     9541 taataaatca aatatatctt gattaaaggc gtgatgccgc cagccattat tgaaaacagc
     9601 ttggttatag tcatccaaat tttgcagaaa atgactttga aaactttcat tgtggtaggc
     9661 gcgtcgacgg ccatatttag cataactgta ttcttcatca cgcagttgtt taactttttc
     9721 ccaaggaatt gtgaatcgtg aatagggcat tttacgtatc atggcgcgac gcattaaagt
     9781 aaacgccaaa gcacgtttat actggttatt cagcttccct atatttttac ggtacaaatc
     9841 aagttgttgg cattcatcgt gaaaatagaa tttttcggaa taacgctgac tcataaaacc
     9901 ggcatgcggg ctgcctgaaa aaatcattgc gacatcatcg tcatttaatg tttcatggtt
     9961 gttttctatt aatgctaaag caatttggta attaattgcc aaaatatcgt tagtaatcac
    10021 acgatagccg cgttttttgg cttcgtaggc aaaggaacag cctccactaa atacatctgc
    10081 aactgtatct acatcagacg gaagctggtc acaaatccag gaagctattt tctctttatt
    10141 accgatatag ttaattttcg gatatttctt attcattttc actcattttt aaaatacttt
    10201 cagcaatagc ttttgccaat aaaggcggca cggcgttacc aacctgctgc tgctgggcaa
    10261 ttttgctgcc gcaaaaaata aaattatcag ggaaagattg taaggcagct aattcacgaa
    10321 cggttaaagc ccgattctgt tcatagtgaa aaactttgcg catatctcct gtaatacaaa
    10381 cggctggttt gttgctgttg taacggatgt atttacggat atcacctgtt ttcggacgta
    10441 atggttcagg aatatcgtta cggttacctc catttttaac aaatgccatt ttttctaaca
    10501 tttgtgccga atgattcata gcttcatgat ttgcaacgtg tggattgctt tcgccagcag
    10561 ccagttttgg aaaatgtcct attgctgatc caacagtctg atgggaaatc tgcaaaggtt
    10621 cgggaaagga aattttgcct ttatccctcc tcccgataaa tatcactcgg ctacgtatct
    10681 gaggaacacc gaaatcggct gcactcagta tcttacattc caccgaataa ccgatattct
    10741 gaaatgcttg aataatctca atacgtgttt tacctgaatt gtgtgtatag agtcgcgcta
    10801 cattttccat aacaaaaaaa tatggttgga caattttaac tattcggaca aactctttaa
    10861 ataaatggtt gcgtgggtca tctgtaaatg tccgtccaat ctttcctgcc atactaaaac
    10921 cttgacaagg tggtcctcca ataaccaaat caactgattg tccgttaaga caattggtta
    10981 aatcttgttc ggttagtgtg gttaaatctt tttgcagtaa ttgatgacgg gggaagttgg
    11041 tacggtaagt ctgacaataa tcagactcca tttcaacaga aagcaattgt tggaatccgg
    11101 cttgttcaaa acccaaggat aggcctcctg ctcctgagaa aaggtcaata taggtaagtg
    11161 gtgtgtgcat aagtcaaaat ccataaactc ttcgattatt ttaccgcttc aacttcgcaa
    11221 acgcatccgc catcgccgaa ttggtcgggg cgcggtcgtt gcgttggggt ttgcggccgg
    11281 ctgtccgttc gcggctgcgg ttttcagacg gcattttatg ttttgcgccg cccggttcgt
    11341 catccaagcg catggtcagc gcgatgcgtt tgcgtgcggc atcgacttcc agcactttca
    11401 cttttaccac gtcgccggct ttcaccacgt cgcgcgggtc ttggacgaac ttattagaca
    11461 gggcggagat gtgcaccaag ccgtcctgat ggacgccgat gtccacgaac gcgccgaagt
    11521 tggcgacgtt ggagaccacg ccctcgagta tcatgccgac ttgcaagtcg ctgatttcgt
    11581 ggatgccttc ggcaaacgat gccgtctgaa acgcaccgcg cgggtcgcgg ccgggttttt
    11641 ccagctcgga caggatgtcc aaaatagtcg gcagaccgaa gcgttcgtcg gtgaagtcgg
    11701 atgctttgat ttgcttcaca cgctcgcggt tgccgatgag ttcggcggcg gtaatgcctt
    11761 gttgcgccag cattttggcg acgacgggat aggcttcggg gtggacggcg ctcgcgtcca
    11821 gcggctcttt gccgccgtta atccgcaaaa agcctgctgc ttgctcgaag gttttttcgc
    11881 ccaagcgcgg tacttttagc aatttttttc ggctgtcgaa cgcgccgttt tcatcacggt
    11941 aggcgacgat gttttgggca agggtttgat tcaagccgga aatccgcgcc aagagcgggg
    12001 cggaggcggt gttcgcgtcc acgccgacgg cgttcacgca gtcttcgacc accgcgtcca
    12061 gcgatttggc gagccggctt tggttcacgt cgtgctgata ctgccccacg ccgatggatt
    12121 tggggtcgat tttgaccaac tcggcaagcg ggtcttgcag cctgcgggcg atggacactg
    12181 cgccgcgcag ggaaacatct aagtcgggaa actcgcgcgc cgccagttcg gacgcagaat
    12241 aaatcgacgc gccggcttcg gaaacgacga ttttgtgcag gctgctttcg ggcattccgc
    12301 gcaccagttc gcccgcgatt ttgtcggttt cgcggctggc ggtgccgttg ccgatggcga
    12361 tgagcttcac accgtgttgt ttaatcaggc gcgacaacgt tgccaacata ttgttttctt
    12421 gatgcaaata gacaatgacc gtatccagca gtttgcccgt gtcgtccacc acggcgcatt
    12481 tcacgccgtt gcggtagccg gggtcgagtc ccaaagtggt cagccgcccg gcgggcgcgg
    12541 cgagcagcaa gtctttgaga ttgcgggcga acacggtaat cgcgtcagtg tcggcagctt
    12601 ctttcaaacg attgagagct tcgagttcca gcgacaaaaa gattttcgcg cgccaagtca
    12661 ggcgcacggt gtcgcgcagc catttgtggc cgtctgaaac cttgaagcgg cgggcgatga
    12721 tttgctcgta ttcgctttgc tgcgtaatcg gcgtgtcgtc gggctggtat ttgagcgcga
    12781 tgttcaacac gccttcgttg cgaccgcgca aaaccgccag cgcgcggtgg ctgggcatgg
    12841 cgcgcacggg ttcgcggcgg tcgaaataat cgctgaattt ttcgccttcg gtttctttgc
    12901 cttcaacgac ttgcgtgtgg atttcggctt cgttccacag cttgtcgcgc agcgtgccga
    12961 tgagttccgc gtcttcggca aactgctcca tcagaatcgc gcgcgcgcca tccagcgcgg
    13021 ctttggcgtc ggggatgttt tcgttcaggt agccttgcgc ggcggcttcc acgtcctgcg
    13081 gctgttcggc aagcaacacg tccgccagcg gctgcaaacc gtgttcgcga gcgatttgcg
    13141 ctttggtgcg gcgtttgggc ttgtagggca gatacaggtc ttccagcgcg gttttgttgt
    13201 cggcggcttc gatttgcgcc ctgaggtcgt ctgaaagctt gccttgctct tcaatgcttt
    13261 ttaacacaac ggccttgcgc tcttccaact cgcgcaggta ttgcagccgc tcggcaagct
    13321 ggcgcagctg cgtatcgtcc agcccgcccg tggcttcttt acggtaacgc gcgataaagg
    13381 ggacggtcgc gccgtcgtcc aaaagctcga tagcggcatt gatttgcacg gtagtggcgg
    13441 agagttcttg ggagaggatt cgggtaatgt tcataaattg cctccgtgat gccgtctgaa
    13501 cagccgacgg cgcaatgtgg gtaatcgttt ggaaaagatg aagataatac tgatatactt
    13561 tgcccgatac aatctgaaag gaatgtttat gaccgtcctg attaccggcg gcaccggctt
    13621 tatcggttcg cacaccgccg tctcgctcgt ccaatccggt tacgatgccg tgattttgga
    13681 taatctgtgc aactcgtctg ccgccgtcct cccgcgcctt cggcaaatta ccggcagaaa
    13741 cataccgttt tatcagggcg acatccgcga ctgtcagatt ttgaggcaga ttttttcaga
    13801 acatgaaatc gaatccgtca tccattttgc cggtttgaag gcagtggggg aaagcgttgc
    13861 cgagccgaca aaatattacg gcaacaatgt ttacggcagc ctggtgctgg cggaagaaat
    13921 ggcgcgcgcg ggcgtgttga aaatcgtatt cagctcgtcg gcaaccgttt acggcgatgc
    13981 ggaaaaagtc ccctatacgg aagatatgcg cccgggcgat accgctaatc cttacggtgc
    14041 gtccaaagcg atggtggagc ggatgttaac cgacatccaa aaagccgatc cgcgttggag
    14101 cgtgattttg ttgcgctatt tcaacccgat cggcgcgcac gaaagcggac ttatcggcga
    14161 acagcccaac ggcgttccca acaatctttt gccctatatc tgtcaagtgg cttcgggcag
    14221 gctgccgcaa ctgtcggtat tcggcggcga ctatccgacc cccgacggta cgggaatgcg
    14281 cgactacatc catgtgatgg atttggcaga agggcatatc gcggcaatga aggcgaaagg
    14341 cggcgttgcc ggcgtacatt tgttcaactt gggttcggga cgcgcctatt ccgttttgga
    14401 aatcatccgc gcctttgagg ccgcatccgg tttgcacatt ccttaccgaa tccaaccccg
    14461 ccgcgccggc gacttggcgt gttcctatgc cgacccgtcc cataccaaac aacaaaccgg
    14521 ctgggaaacc aaacgcggct tgcagcaaat gatggaagat tcgtggcgtt gggtcagccg
    14581 caaccccggc agatatgggg attaaatgca aaccgcaggc aaaaaaaaca tcctcgttac
    14641 cggcggcgcg ggttttatcg gctcggcagt cgtccgccat attatccaaa acacccgaga
    14701 ttccgtcgtc aacctcgaca agttaaccta cgcgggcaat ctcgaatcgc tgaccgacat
    14761 tgccgataat ccccgctacg cttttgagca agtggatatt tgcgaccgcg ccgaactcga
    14821 ccgcgtgttc gcgcaatacc ggcccgatgc cgtgatgcac ttggcggcgg aaagccacgt
    14881 cgaccgcgcc atcggttcgg caggcgaatt tatccgaacc aatatcgtcg gcacattcga
    14941 cctgctggaa gctgcgcgcg cctattggca gcaaatgccg tctgaaaaac gcgaagcctt
    15001 ccgtttccac catatttcta ccgatgaagt ctatggcgat ttgcacggca cagacgattt
    15061 gtttacagaa accacgccat acgcgccgtc cagcccctac tccgcctcca aagccgccgc
    15121 cgaccacctt gtccgcgcgt ggcagcggac ttacaggctg ccctccattg tcagcaactg
    15181 ttccaataat tacggccccc gacaattccc cgaaaaactc attcctttga tgattctgaa
    15241 cgcactttcc ggcaaaccac tgcctgtgta cggcgacggg gcgcaaatcc gcgactggct
    15301 gtttgtcgaa gaccacgcgc gcgcgctgta tcaggtcgtt accgaaggtg ttgtcggcga
    15361 aacctacaat atcggcggac acaatgaaaa aaccaatctc gaagtgatca aaaccatctg
    15421 cgccctcttg gaagaactcg cccccgaaaa accggccggc gtggcacggt atgaagattt
    15481 aattactttc gtacaagacc gccccggcca tgatgcccgc tacgccgtcg atgcggcgaa
    15541 aatcaggcgg gatttgggct ggctgccttt ggaaactttt gaatccggcc tccgcaaaac
    15601 cgtgcaatgg tatttggaca acaaaacccg gcggcaaaac gcataaaacg gcaactgccg
    15661 ttcggaacgc ttaggtacgg gcaaatagtt ttcagacggc atcccgacgc aatgccgtct
    15721 gaaaacccat cgcaaaggaa gaaggaaaag atgaaaggca tcatactggc aggcggcagc
    15781 ggtacgcgcc tatatccgat tacgcgcggc gtatccaaac aactgctgcc cgtgtacgac
    15841 aaaccgatga tttattaccc cttgtcggtt ttgatgctgg cgggaatccg cgatattttg
    15901 gtaattaccg cacccgaaga caacgctgcc ttccaacggc tactcggcga cggctccgac
    15961 ttcggcatcc gcctgcaata tgccgttcaa cccagccccg acggcttggc gcaggcattt
    16021 atcatcggcg aagaatttat cggcaacggc aatgtctgcc tgattttggg cgacaatatt
    16081 ttctacggtc aatcatttac gcaaacattg aaacaggccg ccgccaagac ccacggcgca
    16141 accgtattcg gttatcgggt taaagacccc gagcgtttcg gcgtggttga atttgacgaa
    16201 aacttcaacg ccttatccat cgaagagaaa ccgcaacagc ccaaatccga ttgggcggta
    16261 accggcttgt acttccacga caaccgcgcc gtcgagttcg ccaaacagct caaaccgtcc
    16321 gcacgcggcg aattggaaat ttccgacctc aaccggatgt atctggaaga cggctcgctg
    16381 tccgttcaaa tattgggacg cggtttcgcg tggttggaca ccggcacgca agaaagcctg
    16441 cacgaggcgg cttccttcgt ccaaaccgtg caaaatatcc aaaacctgca catcgcctgc
    16501 ctcgaagaaa tcgcctggcg taacggctgg ctgacgaaaa aagatgtgga aacacgggca
    16561 aagcctttgg aaaaaaccgc ctacgggcaa tacctgctgc gcctgatcgg caaataaata
    16621 ccgtctgaac gccccgacgg ctttcagacg gcatccaaac cgtgccgcgc cggcacctta
    16681 accgacctgc cgcacggcgc gcataaaaaa tcaaaccgca aaaaaacagc ggtaacgata
    16741 ccgccaaagg actttatgga catcatcgac accgccctcc ccgacgtaaa acttttaaaa
    16801 ccgcaagtct tcacagacgg gcgcggtttt tttatggaaa cattccgcga cggatggttc
    16861 aaagaaaata ttgccgatcg aactttcgtg caggaaaacc actccaactc cagcaagggc
    16921 gtattgcgcg gcctgcacta ccaaaccgaa aacacccaag gcaaactcgt acgcatagtt
    16981 gtcggcgaag tgttcgacgt ggccgtcgat atgcgcgaag gttcgccgac tttcggcaaa
    17041 tgggcgggcg caaccttgtc ggcgcaaaac cgataccagc tttggatacc cgaaggcttc
    17101 gcacacggtt tttgcgtttt gggcgacgcg gcggaagtcg tgtacaaatg cacggactat
    17161 tacaaccctg aaaccgaaca ggttttaata tggaacgacc cggcaatcgg cataggctgg
    17221 ccgcttcaaa ccgcgccgct actttcgccc aaagaccttg ccggcaaaac gtgggcacaa
    17281 gccgaaaagc tccgccttac gctttcccga taaaaaatgc cgtccgaacg tttcagacgg
    17341 cattttctcc gacaggctac ctacccgcct tcagtacgcg ctgtgcaaag aaaaacatcc
    17401 cggtaacgaa gaacgccaag cccaaccaag aagcggcggc atcccagttt tgcagattca
    17461 acgcgagcgg cgcatccgag ccgccgttgg tcacagagaa ggcaataata aacgccataa
    17521 ttacaccaat caggctttca ccgacaatca ggcctgccgc gaaaagcgta ccgatacggt
    17581 cggcgttttt cagacggcct tcgcggtttt ccgccttctt gccaatgatg tgtttcaaca
    17641 ccgccgccaa caccgcgcct gccacgatgg gcatattgac ggacggcggc agataaatgc
    17701 ccataccgac cgcaaggacg ggcagggcaa gtttaccgcc tgatgatttt ttcaacacca
    17761 aatcgacgac gattaatacc gctccaatcg cgataccggt aaagatgtac gcccattcga
    17821 ggttgtgggc gaaaatgccc gacgcaatgg tcgtcatcaa agtcgcttga agggctgcca
    17881 aagcctgcgc cgcatccatg ccttcgcgcg gcattgcgcc ggtaaagccg taggcttcgt
    17941 aaagcagttc caacacgggc gaaataacca gcgcaccaac gatacagccg ataatcaggg
    18001 cgacttgctg ccgccaaggc gtagctttga gcaggtagcc ggttttcaag tcttgcaggt
    18061 tgtcgttgga aatcgaagcc acgcagatta ctgccgatcc gcaaaaaagc gtcaacgcca
    18121 gtaagaattt gcggttggcc tcatccgcca acaaacctcc ggattcgcct accagcagca
    18181 aaaccagtga aataacgacg atggacacga tgcccacgcc ggaaatcggg ctggaagacg
    18241 agccgaccaa acctgccata taaccgcagg cggcggcgac caaaaagccg atgacggaag
    18301 ccaaaagcgt gcaaaccgcc accaaaagcc acgccatgcc gcccgtaatg tgcgaatcgc
    18361 cgataaagtg gtaaaacgac acgcctagaa taaacatcat agacaacacc cagaaaatca
    18421 tggctttagg cgacaaatcc tgttcggcgc gttctgtagc gggcgcaccg ccgccaaaac
    18481 tcttgaacga catcttcatg ccttccacca ttggcttgag cagcatcaaa agcgtccaaa
    18541 ccgccgcgat acctatggtt cccgcaccga taaaacgcac tttttccttc cacagcttca
    18601 tcgcaaacgc cgccatttcc atatcggaag gttgcggaat gtgtgaggag aaatacggca
    18661 cggcaatacc ccaagcaatc gaaatgccca gcaggatggc gatgccgccc gtcagtccga
    18721 ccaaataacc cgcgcccaac aaggccagtg aaaagcccat cggcagttgg aaaattgccg
    18781 taccgctttt aaaccaataa ctcgcgctat cggcaatcac acgcagacct ccggcgcaaa
    18841 agctcatcaa tcccgccaac gcgccgccgg ccgccagctc tttgataccg ctgccgccct
    18901 gacggtcatc cccttcttca tgaccgccca ctttcaaaat ttcagcagcc gccacacctt
    18961 ccggataagg caaatcgctt ttcaccacca tcgcgtaacg cagcggaatg gtgaaaatca
    19021 cccccaaaat cccgccggca atacataaaa gcgtcgtctg ccaaaacggg aaaccgctcc
    19081 agtagcccgc catcagcaaa ccgggcagga cgaagatgat ggttgaaagc gtacccgcag
    19141 ccgaggcttg ggtctgcacc atgttgtttt ccaaaatatt gctgcctttg aaaaatttta
    19201 aaaccgccat cgaaatcacc gccgccggaa tcgacgaggc aaaggtcagc ccgactttca
    19261 aaccgaggta aacgttagac gcagtaaaaa ttacagtgat caatgcgccg agtatcatgc
    19321 ctcggagcgt cagttcgcgg tattcttcta ccgaaccgga taaagattta ttcattattc
    19381 ttcctttgac aacagacgtt cacatattgt tggcatcacg ccatgatgtc aagttttaaa
    19441 aagaacagtt aaaaacaatt atcccaccct gcccataccc aattgaaaat aaaaactatt
    19501 tccaaacgaa caaaaacagc cgtatcaggg aggttccccg atacggctgc ttgtttccga
    19561 accttaaaat caatcaaaca aatcgcgcag cttgtctaaa aacgatttct tgcgcggcgt
    19621 ttggttttcc aagcccgtag aaatccgctc aaattcttcc aaaagctctt tttgacggtc
    19681 ggtcaaattg acaggcgttt cgacaacaat atggcagtac aaatcgccgg tcgcgctgct
    19741 gcgtaaagat ttgacaccct tacctttcac gcgcatcctc ctgccggttt gggtttcttt
    19801 ggggacggtg agcttgactt tgccgtccaa ggtcggcact tctaactccc cgcccaaagc
    19861 agccgtggca aaactaatcg gcagttcgca atgcaaatcc aaaccatcgc gttggaaaat
    19921 cttatgcgcc cgaatgcgga cggttacata caggtcgccg gcaggcgcgc cgtgcatacc
    19981 cggcccgcct tcaccgctca aacggatacg ctgcccgtca tcgataccgg cgggaatatt
    20041 gacttccacc gtcttgaccg ccttattccg ccccacgcca cggcatttga cgcaaggttc
    20101 tttaatatgt ttgcccgcac cgcggcaggt cggacaagtc tgctgcatac ggaaaatcgc
    20161 ctgctggatg tgcaccgtac ccgaaccttt gcaagtcggg caggtttccg gggatgcccc
    20221 cggtttcgcg ccgctgccgt tgcagacatc acacgcttca taagtcggaa tattgatgcg
    20281 tttcttcaca ccttttgcgg cttcttcaag cgtgatttcg ataccgacct gcacgtcctc
    20341 accctgataa tcaggctggg cgcgccccga accacctcca aacatttggc tgaaaatatc
    20401 cccaaagtca aaaccctgcg caccgccaaa tccgccaaac cctccgaagc ccccctgtcc
    20461 gccgccttca aacgccgcat gaccatactg gtcgtacatg gcacgctttt ccttgtcgga
    20521 caaagtttca tacgcctttt gtacttcttt aaacttctct tccgcctctt tattgtcagg
    20581 attgcggtcg ggatggtatt tcatcgccaa tttacggtag gcttttttaa tctcatcatc
    20641 ggtagctgct cttgccacac ccagcgtcgc ataaaaatct tgattactca ttttttcatc
    20701 taaaaataaa atcacggctt aaaatgaggg caattgcgca aaacacaaga caaacagac
//
LOCUS       DAXDXW010000033        20685 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXDXW010000033 DAXDXW010000000
VERSION     DAXDXW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20685
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.034"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(126..326)
                     /locus_tag="ACFB64_001055"
     CDS             complement(126..326)
                     /locus_tag="ACFB64_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209425.1"
                     /translation="MPSEGLSDGIFVFVRGIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVIPPLGFQTASYRTVKP"
     gene            complement(340..894)
                     /locus_tag="ACFB64_001056"
     CDS             complement(340..894)
                     /locus_tag="ACFB64_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209426.1"
                     /translation="MKKIIASALIATFALTACQDDTQARLERQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAQAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLIGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPVNYRRPAMRGFGRRR"
     gene            complement(979..1479)
                     /locus_tag="ACFB64_001057"
     CDS             complement(979..1479)
                     /locus_tag="ACFB64_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209427.1"
                     /translation="MDAMPQTAVQNENLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANKRACRFAEKHRLFFTPAQAELSLNKHKNRRLLTIETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            1576..1785
                     /locus_tag="ACFB64_001058"
     CDS             1576..1785
                     /locus_tag="ACFB64_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209428.1"
                     /translation="MGWISSKRKKAPHVCVVPIAVSGCKRFALHDEMPSEDKNIGEIL
                     NQNAAAPQALYRNFFVFFSYPVENI"
     gene            1884..3077
                     /locus_tag="ACFB64_001059"
     CDS             1884..3077
                     /locus_tag="ACFB64_001059"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFC1209429.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVKHSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQAARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCAL
                     DTPYRGRSSEIAATSLKHGVMILVAGADVLRFAPSLLLNDEDIAEGLRRLEHVLTEFA
                     AANRP"
     gene            complement(3184..4428)
                     /gene="clpX"
                     /locus_tag="ACFB64_001060"
     CDS             complement(3184..4428)
                     /gene="clpX"
                     /locus_tag="ACFB64_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC1209430.1"
                     /translation="MSNENRTCSFCGKSKSHAKHLIEGKNAYICDECVANCLEILYEG
                     DNGGTPPENAGGEPEESGKLPTPAEIVANLDDYVIGQGQAKKALAVAVYNHYKRLRHP
                     KADGGVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQHGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTANILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENAGITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGIENVGLEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            4611..4982
                     /gene="rbfA"
                     /locus_tag="ACFB64_001061"
     CDS             4611..4982
                     /gene="rbfA"
                     /locus_tag="ACFB64_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC1209431.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITINEVE
                     VTRDYSHATVFYTVLNQDTREITEEVLEHARGHLRSELSKRIKLFKIPELHFKYDESL
                     ERGMSLSALIDQVAAEKPVED"
     gene            4993..5517
                     /locus_tag="ACFB64_001062"
     CDS             4993..5517
                     /locus_tag="ACFB64_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209432.1"
                     /translation="MNNLIPEHLAAYAHSDNLQIEGGHRCFSLSCQGRDTFHIRYYGE
                     PFDGLITDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSQNQKQNRTLTDL
                     DEYTYRVPIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISIDLID
                     EKHSVRELLNEELS"
     gene            5575..6495
                     /gene="truB"
                     /locus_tag="ACFB64_001063"
     CDS             5575..6495
                     /gene="truB"
                     /locus_tag="ACFB64_001063"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC1209433.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHTG
                     VLDPLATGLLPVCFGEAAKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIAAARADI
                     SLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYSIDIA
                     EFDAPKAVISVRCSKGTYIRTLSEGIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLDETERDNLLLPCDVLVSHFPQTVLNDYAVHMLQCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRMKALRLMNTAASSA"
     gene            6713..7153
                     /locus_tag="ACFB64_001064"
     CDS             6713..7153
                     /locus_tag="ACFB64_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC1209434.1"
                     /translation="MKTGIQTELAQALLSHEKIWANEEKTILAKNILLDLVEKTDPSI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSINNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRNNSQPANYTPN"
     gene            7387..8847
                     /locus_tag="ACFB64_001065"
     CDS             7387..8847
                     /locus_tag="ACFB64_001065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC1209435.1"
                     /translation="MIYIDPPYNTETDSFAYNDKFSHSTWLTFMKNRLEIAKELLKDD
                     GLIFVQCDDKEQAYLKVLLDETFTRENFINCIAVKMSEPSGNKMAHTSHRLPKIKEYI
                     LIYKNKNIKLNPIREQKSEWDNEYNIFLENFTQEDKKFIDLIVNSQTENKEINGNTLK
                     EIDILLKKISPISVNQKLAQLNIKDNEVIKWKLDNAYRIVRTAASSSVKKLADEKKEI
                     CQQQFFSVISKRDKLLYIVKSDYSKDAKAPRVQVLFAEDYLSISLCDLWTNINTTGLE
                     AEGNVELKNGKKPESLIETIIKLATNENDIVLDYHLGSGTTAAVAHKMNRQYIGIEQM
                     DYIETLAVERMKKVIDGEQGGISKAVNWQGGGEFVYAELSPFNETAKQQILACENSDG
                     IKTLFEGLCERHFLKYNVSVNEFSQIIEEPEFQSLALDEQKQMMLEMLDLNQMYISLS
                     EMDDEQFAGCLNDDDKALSRAFYQSVKHQAEKKDGE"
     gene            8837..11611
                     /locus_tag="ACFB64_001066"
     CDS             8837..11611
                     /locus_tag="ACFB64_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC1209436.1"
                     /translation="MANNKTLFDWVEDRKSMLEEMEQTDFFALPEFVSNNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMIAALILYYFEKGYRH
                     FLFFVNKNNIVYKTENNFIDPTHPKFLFTEKILQGDTVIPIRKVETFSQYSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEKEMND
                     RTSDAEIERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENTEVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSGTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYLAFLNWVENVQADDFSFLTTFSTSLNDSNNANEQGKTRTEQALKFMQENK
                     FEFVHLANWIKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYITELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKLD
                     IQNRNELQNKLLKDWQIEFLGLEQDKQVRPDDKLAGCLKILEMVEKHLNESDIPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDIKTAIATQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSQYDVHLIRNEEVFKLNNFADGEGFMPDFVLLLKDKQKSSSDSVDDFLHYQIFI
                     EPKGGHLVENDSWKEAFLKSITVEYGRDKILQKNTPHYRLIGLPFFTDHQKNGQFTEL
                     FPLGAASLEK"
     gene            complement(11760..12932)
                     /locus_tag="ACFB64_001067"
     CDS             complement(11760..12932)
                     /locus_tag="ACFB64_001067"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC1209437.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGGDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTAEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDVSMAFTGHRDIQDVDASILRSKDWGRETV"
     gene            complement(13053..13295)
                     /locus_tag="ACFB64_001068"
     CDS             complement(13053..13295)
                     /locus_tag="ACFB64_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209438.1"
                     /translation="MVSLMVVVARVGFFIVGGIVQYLSVSVKYGGAVADCLFKWRYSL
                     ILLYLFGFLNQLVMFFEERRLGRFAGKRFQNDLIYF"
     gene            13282..13728
                     /gene="iscR"
                     /locus_tag="ACFB64_001069"
     CDS             13282..13728
                     /gene="iscR"
                     /locus_tag="ACFB64_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC1209439.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPPAQINIAQIISAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            13757..14971
                     /locus_tag="ACFB64_001070"
     CDS             13757..14971
                     /locus_tag="ACFB64_001070"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC1209440.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WTAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISIMWVNNEIGVV
                     QNIPAIGEICRERKIAFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            14956..15267
                     /locus_tag="ACFB64_001071"
     CDS             14956..15267
                     /locus_tag="ACFB64_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209441.1"
                     /translation="MGGALRLMSNKGCLRKNTMKVKIINLPNGYKRIIYGKYFEQFDL
                     DYEQDLDVLKKDIEFALSVIEYNRSIFKKFSSLFENKIIFVYQGGHHLDIIDRDKGSL
                     K"
     gene            15573..16067
                     /locus_tag="ACFB64_001072"
     CDS             15573..16067
                     /locus_tag="ACFB64_001072"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HFC1209442.1"
                     /translation="MQPITGNFDLAHLQTIHRELFGNVYDWAGKIRRVDISKGNTRFA
                     NFAFIENESRKLLEKLKNENYLRVLDKDKFAERAAYYLDELNVLHPFREGNGRTLRLF
                     MTQLAIKNGFQIHWQNISAEQMIQACIQAYHADSSLLARLIIRQFRTIVFFRRLKHQQ
                     TTIF"
     gene            16080..16466
                     /gene="iscU"
                     /locus_tag="ACFB64_001073"
     CDS             16080..16466
                     /gene="iscU"
                     /locus_tag="ACFB64_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC1209443.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            16553..16873
                     /gene="iscA"
                     /locus_tag="ACFB64_001074"
     CDS             16553..16873
                     /gene="iscA"
                     /locus_tag="ACFB64_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC1209444.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEANGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEDLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(16833..17054)
                     /locus_tag="ACFB64_001075"
     CDS             complement(16833..17054)
                     /locus_tag="ACFB64_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFC1209445.1"
                     /translation="MGGKAQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKRRDGFDTVPPFLCLKRGSSRRSRRSL"
     gene            17136..17636
                     /gene="hscB"
                     /locus_tag="ACFB64_001076"
     CDS             17136..17636
                     /gene="hscB"
                     /locus_tag="ACFB64_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC1209446.1"
                     /translation="MSQYFTLFRIEPAFDIGTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTSFAPDFLMQQMEWRET
                     LMEARAGNNLESLKNLDNEIRAEQEKLFCGLKQSFARQDCDTAAQQVRQGRFLDKLRH
                     EISSAL"
     gene            17734..20484
                     /gene="gyrA"
                     /locus_tag="ACFB64_001077"
     CDS             17734..20484
                     /gene="gyrA"
                     /locus_tag="ACFB64_001077"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC1209447.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDFAVYATIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLTDTINACLRLLDEPKTEIDELID
                     IIQAPDFPTGATIYGLGGVREGYKTGRGRVVMRGKTHIEPIGKNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKEKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKKQGYYLSEIQADAILRMSLRNLTGLDQKEIIESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     DLEDETADNENTLPSGKNGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGSIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSGASVISNVTEPEA
                     EN"
BASE COUNT         5905 a         5172 c         4772 g         4836 t
ORIGIN      
        1 ccttttccgc tttggcaacg attgaaaatt tccgtaaatt caaatatcta gattccttcc
       61 tgcgtgggaa tgacgcagaa gggtttcaga tgcaggatgg gcattcctgc ctacccaatc
      121 tcgccctacg gcttgacggt tcgatatgat gccgtctgaa aacccaacgg cggtatgaca
      181 atgccgccct gccaacgcac gtagatcaga attgccatcc cgacatcaaa cgcttggaaa
      241 caaaatgccg tctgaaaatc aaacggcaac ataacaatgc ccctaacaaa tacaaaaatg
      301 ccgtctgaaa gcccttcaga cggcattggc gcgccgggtt taccgccttc tgccgaaacc
      361 gcgcatagcg ggacggcggt aattgacggg cgggcgcgtt gtcgggcggt aacgctgcgc
      421 ctgcgccgcc tgttgttttg cacggaggct gcgcgtgttc aaatccctgc tggtgcgcgc
      481 attggggcgt gcggactgat ggtaggcagc acgcgcgcga cgggcgacgg ggctgtcttg
      541 gttgcccgcc cgtgtgaatt tgtttgccag cgcgttgccg ataaacgcgc ctgccgccgc
      601 gccaatcagg ctttgcagca gccagcttcc tgtcgattgg tcgtaaatat actgctgccc
      661 gtctttgccg gtaacgggct gaccgttgtt gccgtttgcc tgcgcctggg caggaatggt
      721 gtctttgact gcttcgggag tcagttggta aaccgtatcg tctgcctgct gtgcgagctg
      781 ctgttgcagg gcttcaatct gtttctgctg ccgttcgagc cgcgcctgcg tgtcgtcttg
      841 gcaggcggtg agtgcgaatg ttgcgataag cgcggaggcg atgatttttt tcatgtgtgt
      901 cctgttcggg tggaaaatcg gttgtattgt atcgccgtcg ggaattttgg caagcattct
      961 gccggcaaat cgtgatgttt acaggggtag ggtgtgcagt ttgcggacaa atgcgaggct
     1021 gttggcgaca gggttgcctt tgttttcgac ttcgtgttcg gtttctatgg tcagcaggcg
     1081 gcggtttttg tgtttgttca ggctcaattc ggcttgtgcg ggtgtgaaaa acaggcggtg
     1141 tttttcggca aagcggcagg cgcgcttgtt tgcggttttc aaaatctgaa gcagcgatac
     1201 gtccagatgg aagcgtcgca cgcccaatac gagaatccgt gcggcaaaaa aatcggcggc
     1261 atcggtaaac tcggcggggc tgctgcggct gaagtcgtgc cgttcggcgg ctatcagggc
     1321 ggcaacggtg cggatttgcg gaatcatcga ttcgctgatg agcgtgtcgt cccgccctgc
     1381 atcgagaagc atgggggaaa aatcctgcgc atcatcgaca ataatgctgc aagtgtgcag
     1441 gttttcgttt tgtacggcgg tttggggcat tgcatccatg gtcattttcc tgattctgtc
     1501 gtgtgttgcc gaatcgggcg acctgtgtga aggtaacaaa aaagccgccc cgttttcgag
     1561 cggcctgttt tgcgtatggg atggatttca agcaagcgca aaaaagcacc gcacgtctgt
     1621 gtggtaccaa tagcagtaag cggttgtaaa cgttttgcct tgcatgatga aatgccgtcc
     1681 gaagataaaa atattgggga gattctaaat caaaacgctg ccgcgcctca agcattatat
     1741 cgaaattttt ttgttttttt cagctatcct gttgaaaata tttaggttta tttttaccac
     1801 tgcccgatat tgtcggcaat ttccctttat ccgccttgaa aaacggtgca taatcccgaa
     1861 caaaaccgca atcaggagca attatgcaaa actatctgac ccccaatttc gcctttgccc
     1921 cgatgattcc cgaacgcgct tcaggcagcc gcgtttggga tacggaaggg cgtgaatata
     1981 ttgatttttc aggcggcatc gccgttaacg ctctggggca ctgccaccct gcccttgtcg
     2041 atgctttaaa cgcgcagatg cacaagctgt ggcacatttc caatatctat acgacgcggc
     2101 cggcgcagga attggcgcaa aaattggtga aacacagttt tgccgacaag gtttttttct
     2161 gcaactcggg cgcggaagcg aatgaggcgg cgttaaagct ggcaaggaaa tatgcgcgcg
     2221 accgtttcgg cggaggcaaa agcgaaatcg tcgcctgtat caacagtttc cacggacgca
     2281 cgctgtttac cgtgtccgtc ggcggacagc cgaaatacag taaggattat gcaccgctgc
     2341 cgcaaggcat tacgcacgtt ccgttcaacg atattgccgc gctggaagct gccgtcggcg
     2401 aacagacctg cgcggtcatc atcgagccga tacagggcga aagcggcatc ctgcccgcca
     2461 ctgcggaata tttgcaggcg gcgcgccgtc tgtgcgaccg gcacaatgcg ttgttgattt
     2521 tggacgaagt tcaaaccggg atggggcata cgggcaggct gtttgcctat gaacattacg
     2581 gcgttgttcc cgatattttg agttcggcaa aagccttggg ctgcggcttt ccgatcggcg
     2641 cgatgctggc gacagaaaag attgccgccg ccttccaacc gggcacgcac ggctcgactt
     2701 tcggcggcaa cccgatggcg tgtgcggtcg gcagccgcgc cttcgacatc atcaacgcgc
     2761 cggaaacctt gcacaacgtc cgcagtcagg ggcagaaact tcagacggca ttgctggatt
     2821 tgggcaggaa aacaggcttg ttctcacagg ttcgcgggat ggggctgctg ctcggctgcg
     2881 cgttggacac gccttatcgc ggacgctcat ccgaaatcgc cgctacctcc ttgaaacacg
     2941 gcgtgatgat cttagttgcg ggtgcggacg tattgcgttt cgcgccttcg ctgctgttga
     3001 acgatgagga tattgcggaa ggtttgcggc gtttggaaca cgtgctgacg gaatttgccg
     3061 ccgcaaaccg cccctaggca gaaagcagat gccgtctgaa gacgggaagg cctcagacgg
     3121 catcagaaac aaaaaaccgc ttcggaaacg tgatccaacg ttccgaagcg gttttgtttg
     3181 ccatcaggac tcgaacacca attccggttc cctgccctct tcgatgactg ccttgccgac
     3241 caccactttt ttcaagcctt gcaaatcggg caggcggtac atcgtatcga gcaggcagcg
     3301 ttcgacgatg gaacgcaggc cgcgcgcgcc ggttttgcgt tccattgcct gccgcgcgat
     3361 ggaacgcaat gcgccttctt caaattccaa cccgacgttt tctatgccga acaaggcttg
     3421 atactgcttg accaaagcgt ttttcggctc ggttaaaata ttaatcagcg cgtcctcatc
     3481 cagttcttct aaagttgcaa tcacgggcaa acgtccgatt aattccggaa tcaggccgaa
     3541 tttgattaaa tcttccggtt cgacgatgcc gaacagcttg gtaatgccgg cattttcgtc
     3601 cttgctgtga acggacgcgc cgaaaccgat accacctttc tcggtgcgct ggcgaatcac
     3661 tttttccaag cctgcaaacg cgccgccgca gataaacagg atgttggcgg tatcaacgtt
     3721 gataaattcc tgattcggat gcttgcgtcc gccttggggc ggaacgcttg ccaccgtacc
     3781 ttcaatcagt ttcagcaagg cttgctgcac gccttcgccg gacacgtcgc gcgtgatgga
     3841 cgggttgtcg cttttacgcg aaattttgtc aatttcgtca atatagacaa tgccgtgctg
     3901 ggctttttcg acatcgaaat cgcatttgcc caacagtttg gtaatgattt gttcgacatc
     3961 ttcgccgaca tagccggctt cggtcagcgt ggtcgcatcc gccatcacga acggcacgtc
     4021 cagtttgcgt gccaaagatt gcgccaacag cgttttaccc gatccggtcg ggccgataag
     4081 caggatgttg gatttcgaca attcgacacc gccgtcggct ttcgggtggc gcaggcgttt
     4141 gtaatggttg taaaccgcaa ccgccagcgc ctttttcgcc tgcccctgcc cgatgacata
     4201 atcgtcgagg ttggcaacga tttcggcggg cgtgggcagt ttgccggatt cttccggctc
     4261 ccctccggca ttttccggag gcgtgccgcc gttgtcgcct tcgtacaata tttcgaggca
     4321 gtttgcgacg cattcgtcgc agatataggc gtttttgccc tcaatcaggt gtttggcgtg
     4381 tgatttggat tttccgcaaa aggaacaggt acggttttcg ttggacatgg ctttctttac
     4441 aatgtatgcg ttgcagaaac ggcatgcgcc gttcgggttg ccaagtataa taactatatc
     4501 cgtgcttatc aacgtattac cttaaaatcc cgccgattcg gctataatac gccctttcgc
     4561 aaccgccccg gcggcagaaa tgccgtctga caccaaatct gaggatattc atgagaaaac
     4621 cccaacgcgg ctacgcccgc caagaccgtg tcaaagaaca aattatgcgc gagcttgccg
     4681 aactcgtccg taccggactg aaagacccgc gcgccggctt catcactatc aacgaagtcg
     4741 aagttacccg cgattacagc cacgccaccg tgttctacac cgttttgaac caagatacgc
     4801 gcgaaattac ggaagaagtg ctggaacacg cgcgcggaca cctccgcagc gaattgtcca
     4861 aacgcatcaa gctgttcaaa atccccgaac tgcatttcaa atacgacgaa tctttggaac
     4921 gcggtatgag cctgtccgcc cttatcgatc aagtggcggc ggaaaaaccg gttgaagact
     4981 gatactgcca gaatgaacaa tctgattccc gaacatttag ccgcctatgc acatagtgat
     5041 aacctgcaaa ttgaaggcgg gcatcgttgc ttttcattat cctgccaagg tagagatact
     5101 ttccacatcc gttactatgg agagcctttt gatggattga ttaccgatac tgataaggcg
     5161 ccggtaaaaa ttgtggcggt agaagctgta agcggcgatg aaatcgtatt gtttgatggg
     5221 gcggaacatg gctataacgc tatgttctgc gacaaatata gccaaaatca aaagcaaaac
     5281 agaacgttaa ctgatttgga tgaatatact taccgagttc cgattcatct ttattacaat
     5341 atagactacg aagatgaata tgaagatttc gtcaattctg aaggacaagt ccccttaatt
     5401 gatggccgca tcattagttt tgactcatta aaacgaaatg gctttgatgc aatcagcatt
     5461 gatctaattg atgaaaaaca ctctgttcgt gaattattga atgaagaatt aagctgacac
     5521 gcaggccgtc cgaacccttt cagacggtct gaacattaat attccctaat taatatgacc
     5581 aataaacccg ccaaacgccc ggtcaacggc gttcttcttc tcgacaaacc cgaaggcctt
     5641 tccagcaaca ccgccctgca aaaagcgcgg cgtttgtttc atgccgaaaa agcagggcat
     5701 accggcgtgc tcgatccttt ggcaaccgga cttttgcccg tctgcttcgg cgaagcggcc
     5761 aagttcgccc aatacctgct ggatgccgat aaagcctaca ccgccacgct gaaactcggc
     5821 gaagccagca gcacgggcga tgccgaagga gaaatcatcg ccgccgcccg cgccgatatt
     5881 tccttagccg aatttcagac ggcctgccaa gcactgacag gcaacatccg ccaagtgccg
     5941 ccgatgtttt ccgccctcaa gcacgaaggc aaaccgttgt acgaatacgc gcgcaaaggc
     6001 attgttatcg aacgcaaacc gcgcgacatt accgtttact ctatcgatat tgccgagttt
     6061 gacgcgccca aagccgtgat aagcgtgcgt tgcagcaaag gcacctacat ccgcaccctc
     6121 agcgaaggca tcgccaaaca catcggcaca ttcgcccacc tgaccgccct acgccgcacc
     6181 gaaaccgccg gctttaccat cgcccaaagc cacacgctcg aagccttggc aaacttggac
     6241 gaaacagaac gcgacaacct gctgctgccc tgcgacgtat tggtttcaca ctttccccaa
     6301 accgttttaa acgattatgc cgtccatatg ctccaatgcg ggcaacggcc gcgtttcgag
     6361 gaagacctgc cttccgatac gccggtacgc gtttacacgg aaaacggccg ctttgtcggt
     6421 ttggcggaat atcaaaagga aatatgccgt atgaaagcct tgcgcctgat gaacacggcg
     6481 gcatcctccg cctgagcggc ggttaaaaat acgggctgcg tttgaataat gtgttgatat
     6541 ttccgtaaaa tcacgacaca ctcggacacc cgccccgctt atcgcaactt tgcgaacttc
     6601 ccccggaaac agcaaagaca tcaaagaatt gattttatta taatttattt gtaaagccat
     6661 ttgccgttac acaagaatgg cacattaaaa taactgatga ggatttataa cgatgaagac
     6721 aggcattcaa accgaattag cccaagccct gctatcacac gaaaaaatat gggcgaacga
     6781 agaaaaaacc attttggcca aaaacatcct gttggatttg gtggaaaaaa ccgacccgtc
     6841 cattatcggt ttgttattga ataatgatga gttaaaacgc catttctttg tggaagtgaa
     6901 tggtgttttg gtgtttaaat tgcaggattt ccgttttttc ttggacaaac acagcatcaa
     6961 taattcctac acaaaatacg ccaaccgcat tggtttgacg gacggcaacc gctttttgaa
     7021 agacaattcg gatattgtgt tggattttcc gtttaaagat tgcgtattga atggcggaca
     7081 aagcaccgaa gaaggcgaag aaatttattt taaacgcaat aatagccagc cagccaatta
     7141 tacaccaaat taaccagaaa aagacaagaa atctttttta atcaaacgct tgcttttgat
     7201 gaaattgacc ggctttttga cgcaaaagca ttctcaaaat tctcccgcca caccgcagac
     7261 ggcaaacaac ccgttggcga aatcaaacgc cgttcagacg gtacgccagc cgaaaatctt
     7321 attatcaaag gcaataatct gattgccctg cattcgcttg ccaagcagtt taaaggcaaa
     7381 gtaaagttga tttatattga tccaccgtat aatacagaaa ccgatagctt tgcatataac
     7441 gataaatttt cacattccac ttggctaaca tttatgaaaa atcgcttgga aattgcgaaa
     7501 gagttattaa aagatgatgg gctaatcttt gttcaatgcg atgataagga acaggcttat
     7561 ttaaaagttt tattagatga aacttttaca agagaaaatt ttataaactg cattgctgta
     7621 aaaatgtcag agccatcagg taacaaaatg gcacatacat cgcatagact accaaagata
     7681 aaggaatata ttcttatcta taagaataaa aatataaaat taaaccctat tagagagcaa
     7741 aaatcagaat gggacaatga gtacaatatt tttttagaaa attttacaca agaagataaa
     7801 aaatttattg atttaatagt aaatagccaa acagaaaata aagaaataaa tggtaatact
     7861 ttaaaagaaa ttgatattct tttaaagaaa atatctccta tttcagttaa tcaaaaatta
     7921 gcccaattaa atattaaaga taatgaagtt ataaaatgga aactagataa tgcgtataga
     7981 attgtaagaa cagcagctag tagtagcgta aaaaaattag cagatgaaaa aaaagaaatt
     8041 tgccaacaac aatttttttc agttatttct aaacgagata aattattgta tatcgtcaag
     8101 tcagattact caaaagatgc aaaagcacca cgagtgcagg ttttgtttgc agaagattat
     8161 ttgagtatta gtttatgcga tttatggaca aatataaata ccacaggact agaagcagaa
     8221 ggaaatgtag agttaaaaaa tggtaaaaag ccagaaagtt taattgaaac catcattaaa
     8281 ctagcaacca acgaaaacga catcgttcta gactaccact tgggcagtgg cacaaccgcc
     8341 gccgtcgccc ataaaatgaa ccgccaatac atcggcattg aacaaatgga ttatattgaa
     8401 acgcttgccg ttgaacgcat gaaaaaagta attgacggcg agcaaggcgg tatctcaaaa
     8461 gctgtgaatt ggcaaggtgg cggcgagttt gtttatgccg aactttcccc atttaacgaa
     8521 accgcaaaac aacaaatttt ggcgtgcgaa aattcagacg gcatcaaaac gctgtttgaa
     8581 ggtttatgcg aacgccattt cttgaaatac aacgtcagcg taaatgaatt tagccaaatt
     8641 attgaagagc ctgaatttca atccttggca ttagacgaac aaaaacaaat gatgcttgaa
     8701 atgttggatt taaatcaaat gtatatttca ttatccgaaa tggacgatga acaatttgca
     8761 ggctgcctga atgatgatga taaagcgtta agccgtgcgt tctatcaatc agtaaaacat
     8821 caagcggaga aaaaagatgg cgaataataa aacgttgttt gattgggtgg aagaccgcaa
     8881 atcaatgctg gaagaaatgg aacagacgga ttttttcgcg ctgcctgaat ttgtttccaa
     8941 taatttaaaa tatccgtttt ttgaatggca aaaatcggct ttagaaaatt ttgtgatttt
     9001 tgaccgcact tcaaaattaa aggatttccc cgacatcaaa aaccgcccta cccatttgct
     9061 gttcaatatg gcaacaggtg caggcaaaac gatgatgata gcggcattga ttctgtatta
     9121 ttttgagaaa gggtatcggc attttttatt tttcgtcaat aaaaacaata tagtatataa
     9181 aacggaaaat aattttatcg acccgacgca tccaaaattt ttatttaccg agaaaatttt
     9241 gcaaggtgat acggtaattc ccattcgcaa agtggagaca tttagccaat attcagacgg
     9301 cattgaaatt aaatttacca gcattcaaaa actatataac gatattcaca ccgagcggga
     9361 aaatcaaacc acattggcgg atttgcacga attaaatctt gtgatgctgg gcgatgaagc
     9421 acaccattta aacgcgcaaa ccaaaggcaa aaaacaaggc gaattagatt tagaaaagga
     9481 aatgaacgac cgtaccagcg atgccgaaat tgaacgcaag ggttgggagc atatggtttt
     9541 ggagttgtta ctcaataaaa atggcaatcc cggccaaaat gtgctgttgg aatttaccgc
     9601 cacgctgcct gaaaatactg aagtgcaaca aaaatatgct gataaaatca tcacaaaatt
     9661 tggtttaaaa gaatttttgc aaaaaggtta taccaaagaa atcaatttgg tatccggtac
     9721 gctgaataag aaagagcggg tgttgcacgc tttattgttt gcctggtacc gccatcaaat
     9781 cgcgttgaaa tacggcattg ccaatttcaa gcctgtgatg ttgtttagaa gtaagacgat
     9841 tgatgaatca aaagcggatt atttggcatt tttaaattgg gtagaaaatg tgcaagcgga
     9901 tgatttttcg tttttaacta cattttcaac aagcttaaac gatagcaata acgccaacga
     9961 acaaggcaaa acccgtaccg aacaagccct gaaatttatg caggaaaata aatttgaatt
    10021 tgtgcatttg gcaaattgga ttaaacaaaa ttatcaaaaa cacaatgtca ttattaccaa
    10081 ctccgaaacc aacaaaaaca aaactgaaaa aaccgacagc gaaaccgaaa aattgctgaa
    10141 caatctggaa gcggctgata atccgatccg tgccattttt acggtggata gattaaccga
    10201 aggttgggac gttttaaatt tgtttgatat tgtgcgtttg tatgaagggc aaaacggtgg
    10261 cggttcgaat aaaaaatcag gcaaaacggc tgccgccacc gtatcggaaa agcagttgat
    10321 tggtcgcggc gtgcgttatt ttccatttgc gtttgaaggt aaacagccga ataaacgcaa
    10381 atttgacaac gatatgcaac atgaattgcg tatcttggaa gaattgtttt attacacgca
    10441 cgatgagcaa tcccgctata ttacagaact gaaaaacgag ttacgaaaag acggttattt
    10501 gcctgaaaaa gacgatgata aggtattggc aacatttaag ctcaaatctg aatttgcaga
    10561 taataaggat tttagagagt tgttaatttg ggcgaataaa aaaatcccca acccaaacgc
    10621 caaaagcaat aatgcagaca gcctgcaagc caatccgcaa acgctttcat tccaagttca
    10681 cggtaatcaa ctgttgcagg aaacgcaatt tacagccgat gaaaatgatg aaacagcccg
    10741 acaaatcggc acacaaaata attttactca aaccataaaa atgagtgaaa tggagcggca
    10801 tattttcaat aaagcattac atatcaaagg gaaaaatagt caatctttat tccattttga
    10861 ccgcctgcaa agcaaactcg acattcaaaa tcgcaatgaa ttgcaaaata aattgttgaa
    10921 agactggcag attgaatttt tgggattaga gcaagataaa caggttcgcc cggatgataa
    10981 acttgcaggc tgcctgaaaa tcttggaaat ggttgaaaaa catctgaatg aaagtgatat
    11041 accgtttatc ggtacaaaag aatttacacc taaaaaattg tgggaaattt tcggcacgcc
    11101 aaaacaaaaa tgggtcaaaa aagatgatat aaaaactgct attgccacgc aaaatgattg
    11161 gtatgtgatg gataattttg ccggaacggg tttggaagaa gcgttaattc aatttatttc
    11221 agcgcgcttg ggcgatttga aatctcaata tgacgttcat ttaatccgca atgaagaagt
    11281 gtttaaattg aataactttg ccgatggcga aggatttatg ccggactttg ttttattgct
    11341 gaaagataaa caaaaatctt cttccgacag cgtggacgac tttttgcatt accaaatttt
    11401 cattgaaccg aaaggcgggc atttagtgga aaatgattcg tggaaagaag cttttttaaa
    11461 atcaatcaca gtggaatatg ggagggataa gatcctgcaa aaaaatacac cgcattatcg
    11521 tttgatcggt ttgccgtttt ttactgatca tcagaaaaat ggacaattta cagagttatt
    11581 ccctttaggt gcggcatcgc ttgaaaaata gagtagtgca ttgcaggcag ccccgttcgg
    11641 caaaacttcc tttacaaaag ggcgttttgt cagatattta atcaacacat tattaaaata
    11701 caaccaaatt ttaatgccgt ccgaaccatg tattcagacg gcatcgtatt tttcagtatc
    11761 taaaccgttt ccctgcccca atccttactc ctcaaaatcg aggcatcgac atcttgaata
    11821 tcgcggtgtc ccgtaaacgc catcgacaca tccatttcct tgtacaaaat ctccagcgcg
    11881 cgcgttacgc cttcttcgcc atacgcaccc aaaccataca ggaacgcccg acctatcatc
    11941 gtgcctttcg cacccaaagc ccacgccttc aaaatatcct ggccgctgcg gataccgctg
    12001 tccatccaaa cttcgatgtc gctgccgact gcggaaacaa tatcgggcaa ggccttgatg
    12061 gcggacacag tatcgtcgag ctggcggccg ccgtggttgg aaacgaccaa tgcatccgca
    12121 ccgcttttcg ccgccttttc cgcgtcttcg ggttccataa tgcctttgat aatcagcttg
    12181 ccgccccaca aatctttaat ccgcgccacg tcgtcccagc ttaagcgcgg gtcgaactgt
    12241 tccgccgtcc acgaagaaag tgaagacaaa tcgccgacgt ttttggcgtg tccgacgata
    12301 ttgcggaacg tgcggcgttc cgtgttcagc attttcatgc accattcggg cttggtcgcc
    12361 agattgatta aattggcgat ggtcggtttc ggcggcgcgg acaggccgtt tttgatgtct
    12421 ttgtggcgtt gacccaaaac ctgcaaatcg gcagtcaata ccaatgccga acatttggca
    12481 tctttcgcac gcttaatcag gttttccata aattcgcggt cgcgcatcac ataaagctga
    12541 aaccaaaacg gcgcgctggt gttctcggca acgtcttcaa tcgagcagat ggacatggtg
    12601 gacaacgtaa acggaatacc gaacttctcc gccgcacgcg ccgccaaaat ttcaccgtct
    12661 gcgtgtgcca tgccggtaaa acccgtcggc gcaatcgcca ccggcatttt cacatccccc
    12721 ccgatcattt tggtttcgag gctgcgtccc tccatattga ccaatacctt ttgtcggaag
    12781 cggatatctt tgaaatccga agtattctcc ctataagtcg tttccgtcca cgaacccgaa
    12841 tcgatgtaat cgtaaaacat acgcggcatt ttgcgcttgg caacgcggcg caagtcttcg
    12901 atgcaggtca ttttgctcaa atcacgtttc atcttgtttt tccctgttta acagcctgat
    12961 ggaatatgcc gtctgaaact gcttgagcct tcaaacggca tttcgggcaa tacccaaata
    13021 aatcactaag atttagataa tctatatcaa tattaaaaat aaatcaaatc gttttgaaac
    13081 cgcttgccgg caaaacgccc caaccgccgc tcctcaaaaa acataactaa ctgattcaaa
    13141 aaaccaaaca aataaagcaa aatcagacta taccgccatt taaacaaaca atcggcaaca
    13201 gctcccccat acttgactga aacactcaga tattggacaa ttccgcccac tataaaaaag
    13261 ccgacacggg caaccaccac catgagactg accaccaaag ggcgtttcgc cgttaccgct
    13321 atgctggatt tggcgatgaa cgcgcaaacc ggcgccgtca aactcagtgc catcagcgaa
    13381 cgccaaaata tatccctctc ctatctcgag caattgttcg gcaaactccg ccgcgccgga
    13441 ctggttgaaa gcctgcgcgg gcccggcggc ggctacatcc tcgccgcccc tcccgcacaa
    13501 atcaacatcg cccaaatcat ttccgccgcc gaagaccgtc tggacgcgac ccaatgcggc
    13561 agcaaagcca actgccacca cggcgcgccc tgcctgacgc acgacctttg ggagaattta
    13621 aacaaaacca tcaacgacta tctcggcagc gttaccctgc aaagcatcat cgaacagaaa
    13681 aacaacggcg acggcagccg cgtcgtccaa tttacacaca tccattaaat aacacccgaa
    13741 aaagaaagag caaaccatga ccgtcaaaac ccccgtttac ctcgactacg ccgccaccac
    13801 acccgttgac aaacgcgttg ccgaaaaaat gattccctat ctgactgaaa ccttcggcaa
    13861 ccccgcctcc aacagccacg cattcggctg gacggcagaa gaagccgtcg aaaaagcccg
    13921 cgccgacatc gccgccctga ttaacgccga ccccaaagaa atcgtcttca ccagcggcgc
    13981 gaccgagtcc gacaacctcg ccatcaaagg tgcggcaaac ttctacaaaa ccaaaggcaa
    14041 acacctcatc accgtcaaaa ccgaacacaa agccgtgctc gacacgatgc gcgaactcga
    14101 acgccaaggc tttgaagtta cctacctcgg cgtgcaggaa aacggtttga ttgatttgga
    14161 agaactcaaa gccgccatcc gcgacgacac catcctgatt tccataatgt gggtgaacaa
    14221 cgaaatcggc gtggtgcaaa acattcccgc catcggcgaa atctgccgcg aacgcaaaat
    14281 cgccttccac gtcgatgccg cccaagcctg cggcaaagtg cctgtcgatg tcgaagccgc
    14341 caaaatcgac ttgctctcga tgtccgcgca caaagtgtac ggccccaaag gcatcggcgc
    14401 gctgtacgtc cgccgcaaac cgcgcgtccg cctcgaagcc caaatgcacg gtggcggtca
    14461 cgagcgcggt ttccgttccg gcacattgcc gacccatcaa atcgtcggca tgggcgaggc
    14521 cttccgcatc gccaaagaag aattggcaca agacacagcg cactacctga aactgcgcga
    14581 catcttcctc aaaggcatcg aaggcatcga agaagtctat atcaacggcg acctcgaaca
    14641 ccgcgccccg aacaacctga acgtcagctt caacttcgtc gaaggcgaaa gcctgattat
    14701 ggcggtgaaa gaactcgccg tatccagcgg atccgcctgc acctccgcct cgctcgaacc
    14761 cagctacgtc ctgcgcgcgc ttggccgcaa cgacgaactg gcgcactcat ccctgcgcat
    14821 caccttcggc cgcatgacca ccgaagaaga agtgcaattc gcggcagaac tgatcaaatc
    14881 caaaatcggc aaactgcgcg aactgtcgcc gctgtgggaa atgttcaaag acggtattga
    14941 tctgaactcg attgaatggg cggcgcatta agattgatgt caaacaaagg ctgtctgaga
    15001 aagaatacca tgaaagttaa aattattaat ttgccaaatg gttataaacg tattatttat
    15061 ggaaaatatt ttgaacaatt tgacttagat tatgaacaag atttagacgt attaaaaaaa
    15121 gatattgaat ttgctttatc tgttattgaa tacaatcgat ctatattcaa aaaattctct
    15181 tccttatttg agaataaaat catttttgtt tatcaaggag gacatcatct tgatattatt
    15241 gatcgtgata aaggaagttt gaaatagtta actattgaag atcattggac aaaagatgtt
    15301 gatgatatca ctggttcatg gaaaatggag aatttagaaa ttagccgaca aactcaatat
    15361 gcaatgcaac ttctaaagat tggtaaaaca aatattgaga aagaaataag aaaaattaaa
    15421 caaaggttct aaaaaaatga gtaaatacgg tggtgaagac gtaatctata tgaatgatgg
    15481 tgtatttaga aataaattag ccattcaaga tccacttaaa tttaaaaaag gtcgaaagag
    15541 acatttctgc tatttccgcc caaaatctgc ttttgcaacc cattacaggc aatttcgatt
    15601 tggcgcattt acagaccatt catcgcgaac tgtttggcaa tgtttatgat tgggcaggaa
    15661 aaatccggag ggttgatatt tctaaaggta atacccgatt tgccaatttt gcttttatcg
    15721 aaaacgaaag ccgtaaactt ttggagaagc tgaaaaacga aaactatttg cgggtgttgg
    15781 ataaagataa attcgccgag cgtgcagcct attatttgga cgagttgaac gtcctacatc
    15841 cttttcgtga aggaaacggc agaacattgc gcctgtttat gacacaattg gcaataaaaa
    15901 acggttttca gatacattgg caaaatatct ctgccgaaca aatgattcaa gcctgtattc
    15961 aggcatacca tgccgatagc agcttattgg cacgcctaat tattagacaa tttagaacaa
    16021 tcgtcttttt cagacgacta aaacatcaac aaaccacgat attttaagga aaccacatca
    16081 tggcatacag cgataaagta atcgaccact acgaaaatcc ccgcaacgtc ggcactttcg
    16141 acaaaaacga cgagtccgtc ggcaccggca tggtcggcgc gcccgcctgc ggcgacgtga
    16201 tgcgcctgca aatcaaagtg aacgatgaag gcatcatcga agatgcgaaa ttcaaaactt
    16261 acggctgcgg ttccgccatc gcttcgtcca gcctgattac cgagtgggtc aaaggcaaaa
    16321 gtctggatga cgcgctggca atcaaaaaca gcgaaatcgc cgaagaactg gaattgccgc
    16381 cggtaaaaat ccactgctcc atcttggctg aagatgcggt aaaagcggcc gttgccgact
    16441 accgcaaacg tcaggaaaac agataaagcc cttcagacgg catcgtccca caatgccgtc
    16501 cgaaccaccc gccgcttcgg atccgtccgg ggcggtacaa caaggaagaa atatgattac
    16561 ccttaccgag aatgccgcaa aacacatcaa tgactatctc gccaaacgcg gcaaaggctt
    16621 gggcgtacgc ttgggtgtaa aaaccagcgg ctgctcgggg atggcgtaca accttgaatt
    16681 tgtcgatgaa gccaacggcg acgacctgat tttcgaagga cacggcgcgc gcatttatat
    16741 cgacccgaaa agcttggttt atctggacgg cacacaagtc gattacacca aagaagattt
    16801 gcaggaaggt ttcaaatttg aaaaccccaa tgtcaaagac tcctgcggct gcggcgagag
    16861 cttccacgtt taaggcataa aaacggcggg acagtatcaa aaccgtcccg ccgttttttc
    16921 gcttcctgcc tgttatagct gcctttgcct ttccttttcc gttccacctt gtgccggaac
    16981 aaatcggatt ttactaaggc ttttaaagca ttgtcgcgta ttttgccttt attgtgctgc
    17041 gctttgccgc ccatattcag tcctttcgtt taagaagccg cagattataa ggcaaaaaca
    17101 gttttctgcc aaaatcttac atttatcatc ctactatgtc ccaatatttc accctcttcc
    17161 ggattgaacc cgctttcgat atcggcaccg aaaacttgga acaaacctac cgcgccttgg
    17221 ccgcccgttt ccatcccgac aaattcgctt ccgcttccgc tttcgagcag aaacaggcgg
    17281 ttatgatgtc ttccaccatc aacgatgcct accgcacctt gaaaaatccc atcgaccgcg
    17341 ccgcgtacct gctgaaaaca tcgggtatcg atgccgatgc gccggaacat acctctttcg
    17401 cccccgactt cctcatgcag caaatggaat ggcgcgaaac gctgatggag gcacgggcag
    17461 gcaacaacct tgaatccttg aaaaatctcg ataacgaaat ccgcgccgaa caagaaaaac
    17521 tgttctgcgg tctgaaacag tcattcgcgc gccaagactg cgacaccgcc gcacaacaag
    17581 tccgccaagg caggtttctc gacaaactcc gccacgaaat ttcctccgca ttataatccg
    17641 caccgtgttt cagacggcat aaccgccgca ccgtttcatg tcgaaatatg ctaaaataag
    17701 caacaatttt ttgccatacg aaacattgaa accatgaccg acgcaaccat ccgccacgac
    17761 cacaaattcg ccctcgaaac cctgcccgtc agccttgaag acgaaatgcg caaaagctat
    17821 ctcgactacg ccatgagcgt cattgtcggg cgcgcgctgc cggacgttcg cgacggccta
    17881 aagccggtgc accggcgcgt actgtacgcg atgcacgagc tgaaaaataa ctggaatgcc
    17941 gcctacaaaa aatcggcgcg catcgtcggc gacgtcatcg gtaaatacca cccccacggc
    18001 gatttcgcag tttacgccac catcgtccgt atggcgcaaa atttcgctat gcgttatgtg
    18061 ctgatagacg gacagggcaa cttcggatcg gtggacgggc ttgccgccgc agccatgcgc
    18121 tataccgaaa tccgcatggc gaaaatctca catgaaatgc tggcagacat tgaggaagaa
    18181 accgttaatt tcggcccgaa ctacgacggt agcgaacacg agccgcttgt actgccgacc
    18241 cgtttcccca cactgctcgt caacggctcg tccggtatcg ccgtcggtat ggcgaccaac
    18301 atcccgccgc acaacctcac cgacaccatc aacgcctgtc tgcgtctttt ggacgaaccc
    18361 aaaaccgaaa tcgacgaact gatcgacatt atccaagccc ccgacttccc gaccggggca
    18421 accatctacg gcttgggcgg cgtgcgcgaa ggctataaaa caggccgcgg ccgcgttgtt
    18481 atgcgcggta agacccatat cgaacccata ggcaaaaacg gcgaacgcga agccatcgtt
    18541 atcgacgaaa tcccctatca ggtcaacaaa gccaagttgg tcgagaaaat cggcgatttg
    18601 gttcgggaaa aaacactgga aggcatttcc gagctccgcg acgaatccga caaatccggt
    18661 atgcgcgtcg ttatcgagct gaaacgcaac gaaaatgccg aagtcgtctt aaaccaactc
    18721 tacaaactga ctccgctgca agacagtttc ggcatcaata tggtggtttt ggtcgacgga
    18781 caaccgcgcc tgttaaacct gaaacagatt ctctccgaat tcctgcgcca ccgccgcgaa
    18841 gtcgttaccc gacgtacgct tttccggctg aagaaggcac gccatgaagg gcatatcgcc
    18901 gaaggcaaag ccgtcgcact gtccaatatc gatgaaatca tcaagctcat caaagaatcg
    18961 cccaacgcgg ccgaggccaa agaaaaactg cttgcgcgcc cttggcgcag cagcctcgtt
    19021 gaagaaatgc tgacgcgttc cggtctggat ttggaaatga tgcgtccgga aggattggct
    19081 gcaaacattg gtctgaaaaa acaaggttat tacctgagcg agattcaggc agatgctatt
    19141 ttacgcatga gcctgcgaaa cctgaccggc ctcgatcaga aagaaattat cgaaagctac
    19201 aaaaacctga tgggtaaaat catcgacttt gtggatatcc tctccaaacc cgaacgcatt
    19261 acccaaatca tccgtgacga actggaagaa atcaaaacca actatggcga cgaacgccgc
    19321 agcgaaatca acccgttcgg cggcgacatt gccgatgaag acctgattcc gcaacgcgaa
    19381 atggtcgtga ccctgaccca cggcggctat ataaaaaccc agccgaccac cgactatcag
    19441 gctcagcgtc gcggcgggcg cggcaaacag gcggctgcca ccaaagacga agactttatc
    19501 gaaaccctgt ttgttgccaa cacgcatgac tatttgatgt gttttaccaa cctcggcaag
    19561 tgccactgga ttaaggttta caaactgccc gaaggcggac gcaacagccg cggccgtccg
    19621 attaacaacg tcatccagct ggaagaaggc gaaaaagtca gcgcgattct ggcagtacgc
    19681 gagtttcccg aagaccaata cgtcttcttc gccaccgcgc agggaatggt gaaaaaagtc
    19741 caactttccg cctttaaaaa cgtccgcgcc caaggcatta aagccatcgc actcaaagaa
    19801 ggcgactacc tcgtcggcgc tgcgcaaaca ggcggtgcgg acgacattat gttgttctcc
    19861 aacttgggca aagccatccg cttcaacgaa tactgggaaa aatccggcaa cgacgaagcg
    19921 gaagatgccg acatcgaaac cgagatttca gacgacctcg aagacgaaac cgccgacaac
    19981 gaaaacaccc tgccaagcgg caaaaacggc gtgcgtccgt ccggtcgcgg cagcggcggt
    20041 ttgcgcggta tgcgcctgcc tgccgacggc aaaatcgtca gcctgattac cttcgcccct
    20101 gaaaccgaag aaagcggttt gcaagtttta accgccaccg ccaacggata cggaaaacgc
    20161 accccgattg ccgattacag ccgcaaaaac aaaggcgggc aaggcagtat tgccattaac
    20221 accggcgagc gcaacggcga tttggtcgcc gcaaccttgg tcggcgaaac cgacgatttg
    20281 atgctgatta ccagcggcgg cgtgcttatc cgtaccaaag tcgaacaaat ccgcgaaacc
    20341 ggccgcgccg cagcaggcgt gaaactgatt aacttggacg aaggcgaaac cttggtatcg
    20401 ctggaacgtg ttgccgaaga cgaatccgaa ctctccggcg cttctgtaat ttccaatgta
    20461 accgaaccgg aagccgagaa ctgaaaatca tctcccgatg ccgtctgaag attcagacgg
    20521 catttatttt tccctcatcc gtcatccagc ttctcacaat atagcggatt aacaaaaatc
    20581 aggataaggc gacgaagccg cagacagtac aaatatagtg gattaaattt aaactagtac
    20641 agcgttgcct cgccttgccg tactatagtc aattaaaaac aaggg
//
LOCUS       DAXDXW010000034        20574 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXDXW010000034 DAXDXW010000000
VERSION     DAXDXW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20574
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.035"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(56..670)
                     /locus_tag="ACFB64_001078"
     CDS             complement(56..670)
                     /locus_tag="ACFB64_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209448.1"
                     /translation="MKKIMFGLLAAMAISNAQAEGNFLNLASKPAKIDGVLEASLDKP
                     YPKFVQDWMKDSKLAGLGRIIQGKIFESTGTIERKFYARSESEHEKTVEDAVGTFKAY
                     CEHHQGTVETLTSDIFCINGRQVQWMGWWYRENYTNFAFSDGYTGEQAYRSKVTDVQP
                     GKKISTSYGSALVLEKGENGLIKIQPNRGSERWITSDSIQVTYK"
     gene            complement(723..1793)
                     /locus_tag="ACFB64_001079"
     CDS             complement(723..1793)
                     /locus_tag="ACFB64_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209449.1"
                     /translation="MSRTNLKGGRKIALWRQTGYIGFYCCLILMMLFFPKDFNRLKII
                     AYTSPDGDEWVTALEAKLKHREGMIYYSRHRPGTRKKMVLTRRKATNFFRYYSEADSG
                     GASAPESLTHLLCKQVLNELSNLPGGLTTVLNCTEHAEQQPPVTIRLNRALSEYRIDI
                     DGKTFYIDVLLEFDQPGNTSLLRHEIRWQRKLAVEIWHTSRLASNAPKCLALSKIGIP
                     VVQIRADKGSFLYIDEDELLNYDNEEIKNRINRHVEKLRNTFRKQILCTLLRNPLSAD
                     FQTALMLHNQIKADEQQAEQIQEKFEALRNKHVLLEAEYSALAAQYAALLEHQNFQAH
                     SGKREIPKKHGILQRMASWFKS"
     gene            complement(1908..2357)
                     /locus_tag="ACFB64_001080"
     CDS             complement(1908..2357)
                     /locus_tag="ACFB64_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HFC1209450.1"
                     /translation="MPDSPMQINGYTIYEHECFTQQLDRLATTIESQKRSEKSSRSEE
                     AKLLERILGEIENIALNPDSPEYRLGKTLGPEYKFWRRAKFMGRYRLFFRYDSNSKII
                     ILAWVNDSGTKRTYGGKNDAYTVFKKMLGNGNPPSDWTALLEVVSKE"
     gene            complement(2359..2670)
                     /locus_tag="ACFB64_001081"
     CDS             complement(2359..2670)
                     /locus_tag="ACFB64_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HFC1209451.1"
                     /translation="MKIIFDAVSTMTSKNQTTIPESVRKALGLEKQDKIRFSVLGGGQ
                     VLLEKNNTEEEEFEHDPVVGKFLHFLENSMTKNPNSIKPTSQSRFNRLRQLTWETDMK
                     D"
     gene            complement(3074..3538)
                     /locus_tag="ACFB64_001082"
     CDS             complement(3074..3538)
                     /locus_tag="ACFB64_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209452.1"
                     /translation="MIENQIDKEITQASCEGRFILKQENGKRFLYLNLPEGSDELNTI
                     WQTDEYDFTVPDLEVSIDVESLHTAVRLLNENQGILHGISTKCSAYSFGFEGKLRYER
                     LDVKPFPIKSFSYYLEFYNDWTGTLYELDLSAFLDEFFGECDPESKLDACLK"
     gene            complement(3627..4406)
                     /locus_tag="ACFB64_001083"
     CDS             complement(3627..4406)
                     /locus_tag="ACFB64_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209453.1"
                     /translation="MPNWCSNSLHFQGNPQRIAEVAEQILIKTDKGYQFSFQHIVPMP
                     EELILPEVPDSYMQQTWFQIAQQPEKKLINLEREMLEQRDKSFWSVQYRLPDELFSEL
                     KLYFSEKFDWDNLTVGEVFLHFEANSEVASRFRYDEPFAKRIAENIRQYGHPNWYDWR
                     LANWGCKWDVNPDCTFVTVGESGIRISCDTAWGPPEGIYRELAKRFPDVEFEAKYLEE
                     GMWFAGTYEGHEGALFDYPCTDDGVRDFATEHFGCEYDDED"
     gene            complement(4977..5549)
                     /locus_tag="ACFB64_001084"
     CDS             complement(4977..5549)
                     /locus_tag="ACFB64_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209454.1"
                     /translation="MPITCGLLAAGAETLLEIVMSIKVDNILLIEGTKESVAEAAKLI
                     LFRSPVHPEHWAFDFNRILPLPENITSDLAKQRIEAWGSSFSPYPTETFVTQREGFIE
                     ICFLTGLAPPFGIYRKLAEIFANLDVCFTAKYINQYGSFGGRYEYRNSVLYHCLCEMA
                     DIRKFGIAEFDIWYEGLSDRLESDGYLVED"
     gene            complement(5584..6165)
                     /locus_tag="ACFB64_001085"
     CDS             complement(5584..6165)
                     /locus_tag="ACFB64_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209455.1"
                     /translation="MQNFIVWMNPDREESAAAAAKLNGAVLTAHTDGVMAAIRRELKR
                     FGLGADLAVFAQTCEEFAKQANADMVCISSSNGDAVAAVTYLEDGYAGKGVSAVADMR
                     YLMPAYRQYFNRPDKMVIFQGALQYLSDEDIAWIFRRHINAIIEWQSNEGLGLYEFGF
                     SLSDVLTDGCRHVLREVVTGWLNREILPELYNV"
     gene            complement(6401..6742)
                     /locus_tag="ACFB64_001086"
     CDS             complement(6401..6742)
                     /locus_tag="ACFB64_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209456.1"
                     /translation="MEQLDSAAPAACDTPSLFDLGRVSMTATVDYRAKEHLPAQLYEN
                     SLYAQILLEAHRHGIWGDIPPEDAKPNQLALKPGEEGRIMSSYKIGDQKIWVVTEWDR
                     SLTTLLFPEDY"
     gene            complement(6819..7940)
                     /locus_tag="ACFB64_001087"
     CDS             complement(6819..7940)
                     /locus_tag="ACFB64_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6094 domain-containing protein"
                     /protein_id="HFC1209457.1"
                     /translation="METQNHLMHPRVAHNHRDNGYFPTDQATLSRIAACLDIPASAGG
                     LLRIFDPCCGTGEALASLASTLQSEKISCQTYGIELEKDRAQEAMSRLTSVIKSDVEN
                     CTMQGSAVGLLFLNPPYGFAAKDNLSNQRTKRLEELFFARTFGTLQPGGVLVMIVPEA
                     ALTEHLTGEIATHCTDVRMIRAAADTYKQLVIFGIRPKNKASISKKQADAQQRLLLDF
                     ENAPETLPAGMPAYCVPIAPNKVFRPRSFKLEQDILERELGQQQSRTLWPSFSQYFGT
                     SAVIAEKRRPLCALGQWHAALALAAGQVNGVVTSTSGRKLLVKGSTHKTKVETKSEEY
                     GKKDRLVVTITSTDRFSPSIRAIDLTEGSSDFGRVLTIK"
     gene            complement(8011..8235)
                     /locus_tag="ACFB64_001088"
     CDS             complement(8011..8235)
                     /locus_tag="ACFB64_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209458.1"
                     /translation="MRYSKNKDYQFFIRQLVSGGEWMFLPKNGRKHSALKHLPTDRKI
                     PIPGSPGQDPRGLLNFKTMVRHIERGGTFD"
     gene            complement(8788..9045)
                     /locus_tag="ACFB64_001089"
     CDS             complement(8788..9045)
                     /locus_tag="ACFB64_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209459.1"
                     /translation="MKFFIFIKPDLPFHKESIESWIKLLEDIGKTALLAMLPLFWLNS
                     YSLTERASGVAVLALVAYFVQVYADKLRKYRDSLNRKDTSC"
     gene            complement(9293..11776)
                     /locus_tag="ACFB64_001090"
     CDS             complement(9293..11776)
                     /locus_tag="ACFB64_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFC1209460.1"
                     /translation="MYNPVSKIDELLPDFAGLDQTVTEMQERQNQITTDFNFIIRQVA
                     ETAQKSTEAAVLPEESPETELPDSDIPLTQFLAEFGDSLFNAVTEQNQPVYQGQTYPL
                     LEEVLDNLERPLFAAQREVVRAVLQQLVAEDKPAAIINAEMGTGKTMMSVAAAAAAHQ
                     AGLHRTLVLSPPHLVYKWRREILKTVPNARVWILNGADTLAKLLQIRALGRKPEVPEF
                     FVMGRVRMRMGYHWRPSYAVRRRRIPSEGASEGFAVSEYYCPKCGCEILDEENKPYNT
                     EGGVKNALAKNRRFCKGNHHGKPCKEPLWTLCRKEQSEDGVVSTVYDRVLKTMTTLPG
                     IGPKTAAKIIGMYGEQYLAEILENNIQAFSNLMDENGEFLFDDRQAARIDRDLGKAEF
                     SLGRGGYQPTEFVKRYLPKNFFGLLVVDEGHEYKNYGTAQGQAMGVLARCCNKILCLT
                     GTLMGGYAEDLFFLLWRLWPQMMIEDGFSYNKGNTLGSASMAFMRKHGVLKDIVRHLG
                     TEYSNGAFSSSKAERNSVRTAKAPGFSPLGIMRYVLPITVFLKLRDLGEGVLPGYKEV
                     FRPVEMTEDQQAVYKNMESVLRQHLKVALRNRDNTLTGVVMSALLSWPDCCYKPQDVY
                     WRRQNKILFHTEPQFDEEKISPKEADMLEVVKDSLAKGRKCLVYTVYTDTRDTTGRLK
                     EIFKRHGIKAAVMKATVKADQREDWVEDQLASGVQVVICNPELVKTGLDLLAFPTIYF
                     LQTGYNVYTLMQAARRSWRIGQKEDVEVYFAGYSDTAQQICLELMGQKVAVTQSTSGD
                     MPDSGLDILNQAEDSVEVQLAKRFVDRQD"
     gene            complement(11872..12363)
                     /locus_tag="ACFB64_001091"
     CDS             complement(11872..12363)
                     /locus_tag="ACFB64_001091"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC1209461.1"
                     /translation="MPLPDFRIRLLDSADDRNAFERPSEPLNRYLQKQVSQDVKRKVA
                     TCFVAVTSDGQILGYYTLAATGVALSNIPAGFRKKLPRYPTVPAVLMGRLAVDGKYGG
                     FGLGSLLLADALKRIGKAEIAAYALIVEAKDEAAIGFYRHFGFTSFEDATKHLFFPLA
                     NLR"
     gene            complement(12365..12649)
                     /locus_tag="ACFB64_001092"
     CDS             complement(12365..12649)
                     /locus_tag="ACFB64_001092"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFC1209462.1"
                     /translation="MFMTATYADCRFEARITSDTRALLKHAAELQGRSMSEFVISAAR
                     EAAQKAVADAEILMLSERDQLQFAQALLNPSKPNGALSRALEKHDELFGR"
     gene            complement(12882..13289)
                     /locus_tag="ACFB64_001093"
     CDS             complement(12882..13289)
                     /locus_tag="ACFB64_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209463.1"
                     /translation="MPTLSEKHEVLSGLNRQDAAAIVALIDLKTEDDMEKVLNKLDFM
                     TQNFDARLDAFEKSVDARISSFEKSVDARISSFEKSVDARFSSFENSVDARISSFENS
                     VDARFSAIDTKINAIYWFIGITTTVAVAAAKFL"
     gene            complement(13515..14096)
                     /locus_tag="ACFB64_001094"
     CDS             complement(13515..14096)
                     /locus_tag="ACFB64_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209464.1"
                     /translation="MLYLTSRNDLMADAFFIWNRQLMFASLHGRDADMLSFQAQLQVS
                     NERLGFRRPEEVLSCPRLTTAEHCLNLSKYMTKYQTLNYGSVTHMFLYSDILIQPNFD
                     SKTGWVMLDDVTADLDKAAWQLVRQLSDIPLLEHWQNAVLSVLEADKSIMRFTPRIDE
                     EYAVVGVQAVRVDIPEDFDVRLTAMLQSGRLHT"
     gene            complement(14106..14621)
                     /locus_tag="ACFB64_001095"
     CDS             complement(14106..14621)
                     /locus_tag="ACFB64_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3275 family protein"
                     /protein_id="HFC1209465.1"
                     /translation="MSINLSGTLTVQVIQGRRGPFKTAKLATSIGTFDVKKPILKQFE
                     PGEYQGNFIIEQLKVKAYEWGGGATSYLDAALDWQHLAEFAQEFSSGSDSPQDDPSDP
                     FDPVPENGIRLPEYAETADQVGEMIAQNREHISLGEYILSDRDELNIARSMMKEAGYS
                     YRPQSQTWCLQ"
     gene            complement(15806..16255)
                     /locus_tag="ACFB64_001096"
     CDS             complement(15806..16255)
                     /locus_tag="ACFB64_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HFC1209466.1"
                     /translation="MTQSNQNYFNLTADGIGFLNRPREVQGKRKSSNYYACTIQASRG
                     DNGEKTRFDLRIVGGQAKELFARLLEEFPQLLSKNYRERPTVVVGFRAGDIQPLKFET
                     NNRETGEVLETLSIDGRLLKFKFIKVDGDMWYQSSSQDETQNNPDAS"
     gene            complement(16519..17091)
                     /locus_tag="ACFB64_001097"
     CDS             complement(16519..17091)
                     /locus_tag="ACFB64_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HFC1209467.1"
                     /translation="MDRRNFLKKFVGVGALYTTGAAGLLLPKDVVAATSMADFWSRDR
                     TINCKRADTGEKHEIRFFQQQNGYDLDAYRNACWLMRDAKDGNAMVQIDVGLLNLMYA
                     MQEWARQSGRSNPVITINSAYRTPRRNATIEGAARNSLHMRGKAVDFTMRGVGIGELE
                     QMAKYYNVGGIGIYNSFVHLDTGRVRHWRG"
     gene            complement(17195..17734)
                     /locus_tag="ACFB64_001098"
     CDS             complement(17195..17734)
                     /locus_tag="ACFB64_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209468.1"
                     /translation="MKKLVKKLSDSIFGNTCIEYVLDENHNYKEQYNANKDLFIYING
                     IQVGSISPGELQELQYRVFYSSSLWWSIFKSILGFLLKPAIIALTALPLGVLLLLWLN
                     TDNPEAFHRLFMMLQDPAHFAKAISGLFRLFYCISFFGTFVKLVFNQDSIFSIFNKEL
                     SKNIQSNKTCSMDIVAVRD"
     gene            complement(17749..19788)
                     /locus_tag="ACFB64_001099"
     CDS             complement(17749..19788)
                     /locus_tag="ACFB64_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFC1209469.1"
                     /translation="MRLFLCEKPSQAKDIGKVLGVLGGRHDGYFQNGDTIVTWAFGHI
                     LTQAAPDAYGEQYADFGNISALPILPEVWQMQVPPKVSKQFKVIKSLLAKADEVVIAT
                     DADREGEVIAREILDYCGYRKKISRFWTSGLDTQSVKKALSSIMPGKDKENLYQAGLA
                     RGRADWLVGMNLSRAYTAAYSAGYGREHILSIGRIQTPTLKLVVQRDKEIDTFVPYNY
                     YTLSVDFETADKSVFTTLWQVPENWRNTDGYCTNLEAVKALVKQLEKSTGKVITSNKE
                     RKSTPPPLPYSLSALQKEAGKKLGLSASKVLKIAQTLYEKYKITSYPRTPCRYLPKSQ
                     MSEVKDVFAAIQTIDKSMLSVIEKADPERQSRVWNDNQVNKHSHHAIIPTKASNFDLS
                     VLDKNELTVYRMIRDRYIAQFYPDFEYDSTVVEVEACSHLFKASSQSPVISGWKVLLG
                     KDVFEGDQIDEGPALPHLKVNDEVDTLSFNPETKKTTPPPRFTEASLLDEMQTLKDFL
                     KNVEDEQIRKILKSTEGLGTEATRATIIDRLFEMGYMEKKRSKIYATEKGRNLIARIP
                     TMIADPITTAKWELALAGIEAGKLTLAEFMAYQQKVITELVGQAKKDAVGKARPPKQT
                     DSAGTKKQAVARNEDDVCPTCKEGRLRQRGFKENPDKRYWGCSRFPECKHFEWVK"
     gene            complement(19980..20411)
                     /locus_tag="ACFB64_001100"
     CDS             complement(19980..20411)
                     /locus_tag="ACFB64_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC1209470.1"
                     /translation="MSVQLFVRGNLGAEFNLRPVASPKNDEASIVLNFSVASPNFKRN
                     AEGKSEVVSTEWISCEYWNRDAAHLHKILQVGMPVFVTGEERYESYTNRDGIEVRVRK
                     LRVQDVFIVPTERVEAIKLRPKREQSQDNNHVEDADDDIPL"
BASE COUNT         5668 a         4820 c         4452 g         5634 t
ORIGIN      
        1 tgtgattggc agcatttcag ctaccaatcc tctttcttat atgatacaca tcccattatt
       61 tataagttac ttggatgctg tcacttgtaa tccaacgttc gctgcctctg ttcggctgaa
      121 ttttaattaa gccattctca cctttttcta agaccaaggc agacccatag gaagtggaga
      181 tttttttacc gggttgtaca tccgtaactt tgctgcggta ggcttgttcc cctgtatatc
      241 catcagagaa tgcaaaattc gtatagtttt ctctgtacca ccatcccatc cactggactt
      301 gtctgccgtt gatacagaaa atatcggaag tcagggtttc aaccgtcccc tgatgatgtt
      361 cgcaatatgc tttgaaagtt cctactgcat cctcaaccgt tttttcatgt tcggattcgg
      421 atcttgcata gaattttcgt tcaatagttc ccgtactttc aaaaatcttg ccttggatga
      481 tacggccaag tcccgccaat ttactgtctt tcatccaatc ttgtacaaac ttgggatagg
      541 gtttgtctaa agaagcttcc aataccccgt caattttggc cggctttgaa gcaagattca
      601 agaaattacc ttcagcttgg gcatttgaaa tggccatagc tgctaataat ccgaacataa
      661 tttttttcat tgcaatattt tcctcatctt attgaaactt agggtttgtt ctaaacagtg
      721 gtttatgatt taaaccaact ggccatgcgc tgcagaatcc catgcttttt cggaatttcc
      781 cttttcccgg agtgagcttg gaaattctga tgttctaata aggctgcgta ttgggcggcc
      841 aaagccgaat attcagcttc caataaaaca tgtttattcc tcagcgcctc aaatttttcc
      901 tgtatttgct ctgcttgttg ttcatcggcc ttaatttgat tatgcaacat caaggccgtc
      961 tgaaaatccg cactcaaagg attacggagt agggtgcaga gaatttgttt ccgaaaggtg
     1021 ttccgaagtt tctctacatg gcgatttatg cggtttttga tttcttcatt atcataattg
     1081 agcagctcgt cttcatcaat atacaaaaat gaacccttat ctgcccgtat ctgaaccacc
     1141 gggataccta ttttcgataa ggctaaacat ttaggcgcat tggaagccaa ccgtgatgta
     1201 tgccaaatct caaccgccaa ttttctctgc caccttatct cgtgcctgag cagggaagtg
     1261 ttacccggct gatcaaattc aagcaaaaca tcaatataaa aagttttgcc atctatatct
     1321 atccgatatt cggataatgc acggttcagc ctaatagtga caggcggttg ttgctcggcg
     1381 tgttctgtgc aatttagaac agtcgtcagg ccgccgggta agttggatag ttcgttcagc
     1441 acttgtttgc aaagcaagtg ggttagcgat tcaggggcgg aagcaccacc ggagtcggct
     1501 tcactgtaat agcgaaaaaa attggtcgcc ttcctcctgg tcaaaaccat tttctttctt
     1561 gtaccgggac gatgacggct gtagtagatc attccttccc ggtgttttag ctttgcttcc
     1621 agcgcagtta cccattcatc accatccgga ctcgtataag caataatttt caaacgatta
     1681 aaatcctttg ggaagaaaag catcatcaga ataagacaac aataaaatcc tatatatcct
     1741 gtttgccgcc aaagggcaat ttttcttccc ccttttagat ttgtccggct catatacgga
     1801 catgggcaac attatagata acagcaatta ttattacaat gttgtaacat tcagtctaat
     1861 actgtatttt gaccatgaga atcttctaag ggctgttttc ttttgctcta ttcctttgat
     1921 actacttcaa gcagtgcagt ccaatctgaa ggaggatttc cgttccccag catttttttg
     1981 aacacagtat atgcatcatt tttgccgcca taagttcttt tcgtacccga gtcgtttacc
     2041 catgcgagaa tgatgatttt actgtttgag tcgtaacgga aaaacaatcg gtatctaccc
     2101 ataaacttgg cacgtcgcca aaatttgtat tccgggccga gtgtcttgcc aagccgatat
     2161 tccgggctat ccggattaag ggcaatattt tctatttcgc ctaagattcg ttccagcagt
     2221 ttggcctctt cggatctact ggatttttcg gatcgtttct ggctttctat cgtagtggcc
     2281 agtctgtcta attgctgcgt aaagcattcg tgttcgtaaa tggtatatcc gtttatctgc
     2341 ataggggaat caggcatatt agtctttcat atctgtttcc caagtcagtt ggcgaaggcg
     2401 gttaaagcgt gattgagatg tgggtttgat ggaattcgga ttttttgtca tagaattttc
     2461 caaaaaatgc aggaattttc caacaactgg atcatgctca aattcttctt cctcagtatt
     2521 gtttttttct agcaatacct gtccgccgcc taagacggaa aaacggattt tgtcctgttt
     2581 ttccagcccc aacgccttgc gtactgactc gggaatggtt gtttggtttt tgctggtcat
     2641 agttgacact gcatcaaaaa taatcttcat ggtctctttg ccctgccttc attcaaattt
     2701 actgttgcta cttgcgactt caatgtccta gttacgcaca tcttctactt aacggcttgg
     2761 gaaattattg atattgttaa aaactgctta aattaaagaa ttctgtttat tgtaaggaaa
     2821 tttccttaca gggtcaatac ccaacccctt cgacctgcaa gggcgtttat gaatacccat
     2881 ttactgactg gcagcgatga cacgcttaag tgagggagca ggcgtaggcc ggaggtaatt
     2941 cctgttcccg agctgtcggc ggcgagaccg ggggattggg ggcagagacc ccaaaaatat
     3001 cagagtatgg gcacaaaaaa acggccgcat tagcggccgt tggttttgaa aaagattatt
     3061 tgaggcacaa atgctacttc aaacaggcat cgagcttgga ttcaggatca cactctccga
     3121 aaaactcatc caggaacgct gataagtcca gttcatacaa cgtccccgtc cagtcgttat
     3181 aaaactccaa ataataagag aacgatttga tagggaaagg ctttacatcc aatctttcgt
     3241 atctcaactt tccttcgaaa ccgaacgaat acgcactgca ttttgtgctg atgccgtgca
     3301 aaatgccctg gttttcgttc aaaagtctaa cagcagtgtg aagggattca acatcgatgc
     3361 tgacttctaa gtcgggtacg gtaaagtcat actcgtccgt ctgccaaatg gtatttaatt
     3421 cgtcactacc ttcgggcagg ttaaggtaaa ggaaacgttt gccgttttcc tgttttagga
     3481 tgaatcggcc ttcgcatgaa gcctgagtga tctctttgtc aatctgattt tcaatcattt
     3541 ctaatctcca caaaaaagcg ggggacaacc ctgcgggttg tccccgcagg gtgagttggc
     3601 aatacgtcta acgaaatcta agttctttaa tcttcgtcat cgtattcgca tccaaagtgc
     3661 tcggtagcga agtcacggac tccatcatct gtgcacggat aatcgaacaa tgctccttca
     3721 tgaccttcat aggttcctgc aaaccacatt ccttcttcca ggtatttggc ttcaaattct
     3781 acgtcaggaa aacgtttggc gagttctcta taaatccctt ccggaggacc ccaggcagtg
     3841 tcacagctga tccggatgcc gctttcccca actgtcacaa aagtgcagtc ggggtttacg
     3901 tcccatttgc agccccagtt agctaaccgc cagtcatacc aattcggatg accgtattgg
     3961 cggatgtttt ccgcaatacg cttggcaaat ggttcatcgt aacggaaacg agatgcgact
     4021 tcagagttag cttcaaaatg gagaaatact tcaccaacag tcagattgtc ccaatcaaat
     4081 ttttccgaaa aatatagctt taattcagag aaaagttcat ccggcaatct atattgaacg
     4141 gaccaaaagg atttatcgcg ctgttccagc atttcccgtt ccaaattgat caacttcttt
     4201 tcaggttgtt gagcaatttg aaaccaggtc tgctgcatgt aactatcagg cacttcagga
     4261 agaatcagct cctccggcat gggtacaata tgctgaaagc tgaattgata ccccttgtcg
     4321 gttttgatca gaatttgctc cgccacttcg gcgatacgtt gaggattgcc ttgaaaatga
     4381 aggctgttgc tgcaccagtt gggcatagta gtctcctgag caggaatccc catccctgcg
     4441 gataaggaaa cccgcaggtt gataggcttg cgcccgggtt gataaaaaag ccgccaccgg
     4501 aaaccggcgc acatttcaga cgacaggtgc tttagataaa aggcaaagaa accgaaacgg
     4561 taactttgcc tttgggaagc ggaacgacgg ttccgccggc ttgatgaaaa atgtgcttaa
     4621 gcacggttat tttttggggg ttttaagata gattctcgca acaataacaa gaaccaggcc
     4681 gactgcggcc atgacccaag ttccggtaac gaagtccatt atttgtcctc cagtttagag
     4741 atgtgaatgc ggataaggaa tgaaacatac agcatttcaa acgacaggag caccaatcct
     4801 gccatggcaa gattcttcat ctcctcacta tatccgttac tccagaccac cacaccgatg
     4861 acggggaata catccccttc agggttgata aaagagccgt gcatccgaaa cggatgtgca
     4921 tttggttaac ggcggtatgc gggcggtttg acggactgcc taccgtgtaa catagtctaa
     4981 tcttctacca aatacccgtc gctttcaagt ctatcggaca agccttcgta ccaaatatcg
     5041 aattcggcga taccgaattt acggatgtcg gccatctcgc aaagacagtg gtaaagcacg
     5101 ctgttacggt attcgtagcg tccgccgaaa cttccgtact ggttgatgta tttcgctgta
     5161 aaacatacat ccaagttggc gaagatttcc gcgagtttgc gataaatacc gaaaggcggt
     5221 gccaatccgg ttaaaaagca gatttcaata aaaccctctc tttgtgtaac aaaggtttcg
     5281 gtgggatatg gggaaaaact gctgccccat gcctcgattc tctgtttggc aaggtcagac
     5341 gtgatatttt cgggtaaagg caaaatccga ttgaaatcaa aagcccaatg ctcaggatga
     5401 acgggggagc gaaataaaat cagtttcgcc gcttcggcaa ccgattcttt ggttccttct
     5461 attagaagga tgttgtcaac tttgatagac ataactatct ccagtagagt ttctgcccct
     5521 gcggccaaaa gaccgcaggt aatgggcatt tacccagttg gaaaagaagc acggcggtta
     5581 tgcctacaca ttgtacagct cgggcaggat ttcgcggttc agccagccgg tgacgacttc
     5641 gcgcagcaca tggcggcagc catcagtcag aacatcagat agactgaaac cgaactcgta
     5701 cagccccagc ccttcattcg actgccactc gatgatggca ttgatgtggc ggcggaaaat
     5761 ccaggcaatg tcttcatctg ataaatactg caatgccccc tgaaaaataa ccattttgtc
     5821 aggacggttg aaatactgcc gataggcagg catcagatag cgcatgtcgg caacggcgga
     5881 tacgccttta ccggcgtatc cgtcctcaag gtaagtgacg gctgcaacgg catcaccatt
     5941 gctactgctg atgcaaacca tgtcggcatt agcctgtttg gcaaactctt cacaggtttg
     6001 ggcaaagacg gccaaatcag cccctaatcc aaatcttttc agttcacgtc taatagcggc
     6061 catgacacca tccgtgtgag cggtcaaaac tgcaccgttc aatttagctg ccgcagctgc
     6121 cgattcttcc cgatcgggat tcatccatac aataaagttt tgcattgtgt tgctccttaa
     6181 aaagaaaacg gagaacacag tccgtaggga ttgtgttccc cacggggttg ataaaagaaa
     6241 atgccgtaca tccataagga tgcgtgtcat gtttaacggc aggacacaag acagataagg
     6301 tctgccagcc tgtaaagaca ggtattttca gacggcctta ctaaagccgt ctgaaaatac
     6361 ctgcccgaag gcaggtgaga tatataaaaa gattacaagg tcagtaatct tctgggaaca
     6421 ggagtgtggt aaggcttctg tcccattctg taacaaccca aattttttgg tccccaatct
     6481 tgtaagaaga cattattctg ccttcttcac ctggcttgag tgccagctga ttcggcttgg
     6541 catcctcagg aggaatatca ccccatatgc catggcggtg ggcctcaagc aatatttggg
     6601 cataaagact gttctcatac agttgtgcag gcaggtgctc ttttgcccgg taatctactg
     6661 ttgccgtcat agacactcgg cccaggtcaa acagagaggg ggtatcacat gcggccggag
     6721 ctgccgaatc aagttgttcc attaggcgta tccttaaaaa aaccggatac acccctgcgg
     6781 gaagtacccg gcagggttta aaaattgaat aggccaatct atttgattgt caggactcgt
     6841 ccaaaatccg acgatccttc ggtcaaatca attgcgcgta tgcttggaga aaaccggtca
     6901 gttgaagtaa tcgtgaccac aagcctgtcc ttcttgccgt actcttccga tttggtttcc
     6961 actttggttt tatgggttga acctttaacc agcagctttc ggccactggt tgaggtgaca
     7021 acgccattaa cttgccctgc ggctaatgct aaagcagcat gccactgacc cagagcacat
     7081 aaaggccggc gtttttcggc tataacggca ctggtgccga aatattgcga aaatgatggc
     7141 cataacgtgc gggattgctg ctgtcccagt tcacgttcca atatatcctg ctccaattta
     7201 aagcttctgg ggcgaaagac cttattgggt gcaataggca cacagtatgc aggcataccg
     7261 gccggcaaag tctcaggggc attttcaaaa tcaagcagga gcctttgttg ggcatccgcc
     7321 tgttttttgc tgatagaggc tttgttcttc ggtcggatgc cgaatataac caactgtttg
     7381 taggtatccg ccgcagccct tatcatacgg acatccgtac agtgggtagc gatttcgccg
     7441 gttagatgct ctgtcagggc ggcttccgga acaatcatta ccaacactcc gccgggctgc
     7501 aaagtaccga aggtacgtgc gaagaaaagt tcttcaagcc gtttggttct ttgattagac
     7561 agattgtctt tggcggcaaa gccgtaagga ggattcagaa acaggaggcc gactgccgaa
     7621 ccctgcatgg tgcagttttc tacatcggat ttgatgacac tggtcagtcg gctcattgcc
     7681 tcttgggcac ggtctttctc cagttcaatc ccgtaagttt gacaacttat tttttccgat
     7741 tgcaaagtag aagctaaaga agccagggct tctcctgttc cgcagcaagg atcaaatatg
     7801 cgtaacaaac ctccggcaga tgccggaata tcaaggcaag cggctatacg cgacaatgtg
     7861 gcctggtcgg tggggaagta tccgttgtca cggtggttat gcgcgacacg ggggtgcatc
     7921 agatggtttt gagtttccat attggtctcc attcaaataa aaaacggaga catgccgtgc
     7981 gggacagtcc ccgcagggtt aaatcaaagt ttaatcgaag gtaccgccac gttctatatg
     8041 acgtaccatt gttttaaaat tcagcaatcc acgcggatcc tgtcccggcg agccgggtat
     8101 cggaattttt cgatcggtcg gtaggtgctt caatgcgctg tgcttacgac cgtttttggg
     8161 gaggaacatc cattctcccc ccgataccag ttggcgtata aaaaattgat aatctttgtt
     8221 tttgctatat ctcatttctt ctccgataca aaaataggtt ttcctcctgc aaatggttaa
     8281 aaatacacgc atacgattaa aaaaaacgta accgcatgac ttccaagaaa aatcccactg
     8341 ccgtacagac gtacggaaca gcgggattgc ggcttcaagc cggatttatc taaaaaggaa
     8401 cgtatcagcg ttctatttaa aatgctttgg tattaaatag caacaaagca tttaaaagat
     8461 gggaatacca attaacttga ccggtcatgt gtattagtgt cttgctgaga ctcgccaaac
     8521 tttaccagtt taaatatgcc aaacaacatg acggcaatca aaaatagtaa gctgtaggtc
     8581 atgatcatgg tttttccctt tccttgggtt cctgtttgac aatctttgat tcatatttat
     8641 ccaagtaaaa gataaatact tgagctacat agaatgaacc gcgttaacgg tttttgtgtt
     8701 cttccgacgt acgcgaagtt tcaagctgct tggtaatcca atagccgaaa gcaagcacca
     8761 ggaagagaat aaatagcaga ccgcctatca gcatgacgta tcctttctgt ttaatgaatc
     8821 acggtatttc cgtagtttgt cagcatatac ttgcacaaag taggcaacca aagccaatac
     8881 ggctacgccg gaagcacgtt cggtcaaaga gtatgaattg agccaaaaca acggcagcat
     8941 tgccaacaag gctgttttac ctatatcttc cagcaatttg atccaagatt caatgctttc
     9001 cttgtgaaaa ggaaggtcgg gtttgatgaa tataaaaaat ttcacatcgg tatctccatg
     9061 aaaaagggag atataccgat gccctgtggg gatacggtat atccccacag ggttaaagaa
     9121 agttgaaaaa gagccgtccg tcccgaagga cgtgcgcaat gggtaacggc agtgcgcggg
     9181 cagataaggt ctgccagcct gtgaaattcc accttacggg ctttttcaga agacctcaac
     9241 acgaggtcgt ctgaaaaaac ccactaaacg cgggttagat gcaaaaattg catcaatctt
     9301 gtcggtcaac gaagcgtttg gccaactgaa cttcaactga atcttcagcc tgattcagaa
     9361 tgtccagacc cgaatcgggc atatcaccgg aggttgactg agtaacggca actttttgcc
     9421 ccatcagttc aaggcaaatt tgctgcgctg tatcgctgta accggcgaaa tacacttcca
     9481 cgtcttcctt ctgaccaata cgccaagaac ggcgggcagc ctgcatcagg gtataaacgt
     9541 tatatcctgt ttgcaaaaag tagatggttg gaaatgccaa caagtccaaa ccggttttga
     9601 ccagctccgg attacagata acaacctgta cgcctgaagc caattggtct tcaacccagt
     9661 cttcgcgttg atcggctttg accgtcgctt tcattaccgc cgccttaata ccgtgccgtt
     9721 tgaaaatctc tttcagacgg cctgtcgtat cacgggtatc ggtataaaca gtatagacca
     9781 gacatttacg tcctttagcc agactgtctt taacgacttc cagcatgtca gcttctttcg
     9841 gtgagatttt ttcttcatcg aactggggtt cggtatggaa aagaatcttg ttttgtctgc
     9901 gccagtaaac atcttgaggt ttgtaacaac aatccggcca tgacagcaag gcgctcataa
     9961 caacgccggt aagggtattg tcacgattcc ttaatgccac cttcaaatgc tgcctcaaaa
    10021 cactctccat atttttatag accgcctgtt gatcttcagt catttcaacc ggccggaaaa
    10081 cttctttata gccaggtaaa acgccttctc ccaaatcacg cagcttaaga aatacggtaa
    10141 taggcaaaac ataacgcatg atgcccaaag gggaaaatcc cggcgccttg gcggtgcgaa
    10201 cgctgttacg ctctgctttt gagctactga atgcgccatt actgtattca gttccaagat
    10261 gtcggacaat atccttcaaa acgccatgct tacgcataaa cgccatgctt gccgaaccca
    10321 aggtattccc tttgttgtaa ctgaaaccgt cttcaatcat catttgcggc caaagccgcc
    10381 acaatagaaa gaacaggtct tccgcataac cacccatcag cgtaccggtt aagcacagga
    10441 tcttattaca gcaacgtgcc aaaacgccca tggcttgacc ttgagccgta ccgtagttct
    10501 tgtactcgtg accttcatct actaccaaca gcccgaaaaa gttcttcggt aaatagcgtt
    10561 tgacgaactc ggtcggctga tacccgcctc gtcccaggct gaattctgct ttgcctaagt
    10621 ctcggtcgat acgcgccgcc tgacggtcgt cgaataaaaa ttctccgttt tcatccatca
    10681 aattggaaaa cgcctgaatg ttgttttcca agatttcagc cagatactgc tcaccataca
    10741 tcccgatgat ttttgctgcg gttttaggac cgatgccggg tagggtggtc atggttttaa
    10801 gtacgcgatc gtacactgtg cttacaaccc cgtcttcact ctgttccttg cggcacaagg
    10861 tccagagcgg ttccttacac ggtttgccat gatgattgcc tttgcaaaaa cggcggtttt
    10921 tagccagggc gtttttcaca ccaccttcag tgttataagg cttgttttcc tcatccaaga
    10981 tttcacagcc acatttagga cagtaatact cggaaacggc aaaaccctca gaagcacctt
    11041 cagaaggtat ccggcggcgg cgaacggcat acgaaggcct ccaatggtag cccatgcgca
    11101 tccgaacacg ccccatcaca aaaaactccg gaacttcggg tttgcggcca agggcacgga
    11161 tttgcaacag ttttgccaat gtatcggctc cgttaagaat ccatacgcgg gcattgggta
    11221 ccgttttgag gatctctcgt ctccacttgt acaccaaatg aggaggactc aataccaagg
    11281 tgcggtgcaa acctgcttga tgcgcggcag cggctgctgc aaccgacatc atggtcttac
    11341 ctgtgcccat ttcggcatta ataatggctg cgggtttgtc ctctgccacc aattgctgta
    11401 atacggcgcg cacaacttcg cgctgcgccg caaacaaagg gcgttccagg ttatccagca
    11461 cttcttcaag gagaggataa gtttgtccct gatagaccgg ttggttttgc tcagtaactg
    11521 cgttaaagag actatcccca aactcggcaa gaaactgtgt caggggtatg tcggaatccg
    11581 gcagttcagt ttccggagat tcctccggca atacggcagc ttccgtactt ttttgggcag
    11641 tctcagccac ttggcggatg ataaagttga aatcagtagt tatttgattc tgacgttcct
    11701 gcatctcggt cacagtttga tccagccctg caaaatccgg gaggagttcg tctatttttg
    11761 atacggggtt atacatcgta tgctcctaaa aaaaagacgg agcaaacccc ggcgacgggg
    11821 gattgccccg tcagggttag aaaccgctac cgtatggtgc ggaaaaaggg gttagcgaag
    11881 attcgccaaa ggaaaaaata gatgcttggt ggcatcttcg aaagaagtaa agccgaaatg
    11941 cctgtaaaag ccgatggcag cttcgtcttt tgcctccaca atcaacgcat acgcggcgat
    12001 ttctgcttta ccgatacgtt tgagtgcatc agccaaaagt aaagaaccca gtccaaaccc
    12061 gccatatttt ccatctaccg ccaaacggcc catcagcact gcgggaaccg tagggtatct
    12121 gggcagcttc ttacgaaaac cggcgggtat attacttagg gcaactcctg ttgcagccaa
    12181 tgtatagtag ccaagaattt gaccgtcgga tgtaaccgcg acaaaacaag tagcgacttt
    12241 ccgttttacg tcttgcgaaa cctgtttttg cagatagcgg tttaaaggtt cggaagggcg
    12301 ctcaaatgca ttgcggtcat cggcagaatc aaggaggcgg attcggaagt ccggtaacgg
    12361 cattttatct cccgaacaat tcgtcatgtt tttccaatgc tctgctcaat gccccattcg
    12421 gtttggacgg attgagcagg gcttgggcaa actgaagttg gtcacgttcg gacagcatca
    12481 gaatctccgc atcggcaact gctttttggg cagcctcgcg ggcagcactg atgacaaatt
    12541 cggacatact gcgtccttga agttccgcag cgtgtttaag taaagcacgg gtatctgaag
    12601 tgattcttgc ttcaaaacgg caatcggcat atgtggcagt catgaacata ctcctaaatt
    12661 atttctagat tgtacggctg attaccgtac aaaacaagat atgaaagggt attttgtaga
    12721 aaacatcaca tgctccaaag taacggagat atacgatctc catagggatg tatatcccca
    12781 tagggttgag tgagaaatga taaaaatccc accggatgcc ggaacatggt gggattgaag
    12841 gttacagata aaaagctccg tttaacggaa cgggtttaat gttacaggaa cttcgcggca
    12901 gcaacggcaa cggtagtcgt gataccgata aaccaataaa ttgcattgat tttagtatcg
    12961 attgcgctaa accgtgcatc tacgctattt tcaaatgagc tgatgcgggc atctacacta
    13021 ttttcaaatg agctaaaccg tgcatctaca cttttttcaa atgagctgat acgggcatct
    13081 acactttttt caaatgagct gatgcgggca tctacacttt tttcaaaagc gtccagtcgg
    13141 gcatcgaagt tctgggtcat gaaatccagt ttgttcaaaa ccttttccat gtcgtcctcc
    13201 gttttcaaat cgattaaggc tactatagca gctgcatcct gacggttcaa gccggacagt
    13261 acctcatgct tttcagatag agtaggcata aaatcctcct aagtttggga atatgcctcc
    13321 ccatgggacg tatatcccca cagggttggt aaaattgaaa aatgccgcaa ccatacgaat
    13381 acggccggat aacctcagca gcaatatcca tcaacggaat atacagtccg tcatctgtgt
    13441 aaaactctta tgtaacagac tttttcagag agcctttgca aggccgtctg aaaaagcccg
    13501 ctaggggctt cagattaagt atgtaagcgg ccgctttgta gcatagcggt caaacgcaca
    13561 tcaaaatctt ccgggatatc aactcgtacc gcttgaactc caactactgc gtactcttca
    13621 tctatacgag gagtaaaccg catgatggat ttatcagcct caagtaccga taaaacagca
    13681 ttctgccaat gttccaataa cggtatatcg gagagttgcc gcaccagttg ccaagctgcc
    13741 ttgtccaagt ctgcggtaac atcatccaac ataacccaac ctgttttact atcgaagtta
    13801 ggttgaatca gaatatccga atacaaaaac atgtgcgtga ccgaaccata attaagagtc
    13861 tgatatttcg tcatgtattt gctcagattc aagcaatgct cggcagtcgt caggcgcgga
    13921 cagcttaaaa cctcctccgg ccgtctaaaa ccaagccgtt cattacttac ttgcagttga
    13981 gcttggaagg acaacatgtc tgcatcgcgg ccgtgcagag aagcaaacat caactgtcgg
    14041 ttccaaatga agaaggcatc ggccatcaaa tcattgcggg aagtcaggta aagcattcca
    14101 acctcctatt gcagacacca agtctgcgac tggggccgat aggaataccc tgcctccttc
    14161 atcattgaac gggctatgtt cagctcgtca cggtctgaaa ggatatactc acccaaactg
    14221 atatgctccc ggttttgggc gatcatttcg ccgacctgat ccgcagtttc cgcgtattca
    14281 ggcaaccgga tgccgttttc aggaaccgga tcgaacggat cggatggatc atcttgcgga
    14341 gaatccgacc cggaagaaaa ttcctgcgca aattccgcca gatgttgcca gtcaagagct
    14401 gcatctaagt aagaggtggc gccaccaccc cactcataag ccttaacttt gagctgttca
    14461 ataatgaagt ttccttggta ttcaccaggc tcaaactgtt tcaatattgg tttttttaca
    14521 tcaaatgttc caattgatgt agctaattta gctgttttga aagggcctcg gcgaccctgt
    14581 atgacttgca ccgtcaaggt gccggaaaga ttgatggaca tggtatcact ccaaaagaaa
    14641 agggataccg gccgcgggca gatatcccgt ggttgggaaa aaacaaatta gcgcttaata
    14701 gcactgtaaa tcttatccaa atatactttt tgggacatac gcagtcccaa tcttgtaaaa
    14761 atacgtttgt cgttatccaa tgcccgaata gccatattca acgctacgga agcgttttta
    14821 tggctatgaa catcgttgac gctcctacaa taccgattga ccgccttttg ggttcgtatt
    14881 ttcgaaatac ggccatccgc atcaacaagt aacaaatgat gttcttgtat gcatgagacg
    14941 acaactttcc gatgagtatg tcggtgggtg aggatgtcac cgatacgtat tctttccggt
    15001 agtaaatttg ggggaaggat gacacctgaa cggtgaaaag atagatttag attcatggta
    15061 atactccaaa ataaagggta caccatgccc attcggggaa gtgtaccccg atgggttaaa
    15121 agaaatctgc ttcagaaaaa agcagctgta ccgtacctga aacaggcttg caatcaacgg
    15181 ctatgcaatg acagaaaaaa cagtctgtca gctgtataaa actcttgaaa tacagattct
    15241 ctgagccgtt ctgcaaatat caaaacgtct tagagaacct gcaaagcagg ttcaaatggg
    15301 gattaccctg gctaatgatt acctagtccg caccgacgaa gtaatcagat aatggtctgg
    15361 atagtaataa aaccgtaaca attacgaata attgcgcgca ctggttatac ggctatacgc
    15421 agacagaaga aacggtctgt caaccgggga agcttacctg aatgacctat ggtagggagg
    15481 ggcattcagg taagctgccc aaagggcagc cttcaaacag ttgcttcaaa agcaacaaaa
    15541 ataaaaaatt cggattgtta aagaactgta tttttttact gatgctcaca atagatatga
    15601 gcatcgataa aaaaacaata ttgctgaaag ccaagtcttt gaggggactt accctttgct
    15661 tcactataat tcaggagttg cttggaaaat taaaccgccg tttcgacaga ggcttaattt
    15721 tccaagcaat gagctggaaa actaagaacg gggaagcccg aaagcttcct accattctta
    15781 aataatcaaa atattcaata gctttctaag aagcatcagg attattttgg gtttcatctt
    15841 gagaggatga ttgataccac atatcgccat ccactttaat aaatttgaat ttcaacaacc
    15901 gaccgtcgat acttaaagtt tctaaaactt cgcccgtctc acgattgtta gtttcaaatt
    15961 ttaaaggttg aatatcaccg gcacggaaac cgaccacgac agtcgggcgt tcacggtaat
    16021 ttttagacag caattgcgga aattcttcca aaaggcgagc aaagagctct ttcgcttgac
    16081 ctccgacgat acgaagatcg aaacgggttt tctccccatt atcacctcgg gaggcttgga
    16141 tcgtacaagc ataatagttg gaagattttc tctttccttg aacttcacgt ggccggttta
    16201 aaaaaccgat accatctgca gtcaggttaa agtagttttg attagattga gtcatgatat
    16261 atctccaaaa aaacagggga tatatcaatc ccaaagggga cagtatatcc ccaaagggtt
    16321 aagttgcggc tttcacggta gccgaactga cttagaagcc ttaaaggctg gctgaaaaag
    16381 aagtaccgta aattcgacga atcgaatctc attcatgaac ggctaaatga cgagactttt
    16441 aagaaaagtc acaaactaca aaaacgatta caatatattc ccgataattt cgtatgtcaa
    16501 tattttttcg taaaatcttt aaccgcgcca atgccgaacc ctgccggtat ccaaatggac
    16561 aaaactatta tagataccaa taccgcccac attgtagtat ttagccattt gctcaagctc
    16621 gccaatgccc acccccctca tagtaaagtc aactgcttta ccacgcatat gaagtgagtt
    16681 tctagcagca ccctcaatag tcgcatttcg tcttggcgtt ctatatgcac tattgatcgt
    16741 aatcacaggg tttgaccgtc ctgattgccg tgcccactcc tgcattgcat acatcaggtt
    16801 taaaagccct acgtcaattt gaaccatggc atttccatct ttcgcatcac gcatcagcca
    16861 acaggcattg cggtaagcat caagatcata tccattttgt tgctgaaaaa aacggatttc
    16921 atgtttctcg ccagtatctg cccgtttgca attgattgta cggtctcgtg accaaaaatc
    16981 agccatactg gttgccgcta ctacatcttt aggcaataac aaaccggcag caccggtcgt
    17041 atatagggcg ccaacgccga caaacttttt caaaaaattt cttctatcca tctgaaattc
    17101 caaaccaatg actccaatca aattttaagc aacactcagt attgatatgc aaacttccca
    17161 gtatgctggt agagggcaac atctttgagc ttcctcaatc tctgacagcc acaatatcca
    17221 tcgagcacgt tttgttactc tgtatattct tagaaagttc tttattaaaa attgaaaaaa
    17281 ttgaatcttg gttaaaaacc aatttaacaa aagtgccaaa gaatgatata caataaaaca
    17341 gacgaaaaag tccggatatt gcttttgcaa aatgtgccgg atcctgtaac atcatgaata
    17401 atcgatgaaa cgcttcagga ttgtcggtgt tcaaccacag taaaagcaag acccccagcg
    17461 gcagggcagt aagcgcaatt attgcgggtt tcaataaaaa tcctagtata gatttaaaga
    17521 tactccacca gaggctacta gaataaaaca ctctatattg caattcttgc agttcgccag
    17581 gtgaaatact ccctacttga atgccattga tgtaaatgaa taaatcttta ttagcgttgt
    17641 attgctcctt gtaattatgg ttttcatcta gcacatactc tatacaagta tttccgaata
    17701 ttgaatcgga taattttttt accagcttct tcatttcaaa gtcctttttt atttgaccca
    17761 ttcgaagtgt ttacattcgg gaaacctact acacccccaa taccgcttat cagggttttc
    17821 cttgaatcct ctctgtctca gacggccttc cttacaggtc ggacagacat cgtcttcatt
    17881 tcgagcgacc gcctgttttt ttgtaccagc tgaatccgtc tgttttggcg gacgagcttt
    17941 tcctacagca tcctttttcg cctgcccaac cagctctgtg atcactttct gctgataagc
    18001 cataaattca gctaaagtta atttgccggc ttctatccca gccaaagcca gctcccattt
    18061 ggctgtcgtt atcggatcgg caatcatagt gggaatacgg gcaatcagat tacggccttt
    18121 ttctgtcgca tagattttgc tccgtttctt ttccatataa cccatttcaa acaaacgatc
    18181 gataatggta gcgcgagtgg cttccgttcc caagccttcc gtacttttga gaatttttct
    18241 gatctgttcg tcttctacat tcttcaaaaa gtccttcaaa gtctgcatct catcgagtag
    18301 agaagcctct gtaaagcgcg gtggcggcgt ggtcttttta gtttcgggat tgaaggaaag
    18361 cgtgtcgacc tcatcgttga ctttcagatg tggtagagca ggaccttcat cgatttgatc
    18421 gccttcaaac acgtccttgc ccaaaagaac cttccaaccg gaaataaccg gtgattggct
    18481 ggaggcttta aacaaatgtg aacatgcctc aacctcaact acagtagagt catattcaaa
    18541 atctggataa aactgtgcga tataacggtc tcgtatcatt cggtacaccg ttaattcatt
    18601 cttatccagg actgacaaat caaaattact ggccttggtc ggaataatcg catgatggga
    18661 atgtttgttt acctggttat cgttccatac ccttgattga cgttcaggat cagctttttc
    18721 aatcactgaa agcatggatt tatcgatagt ctgtatcgcg gcaaatacat ctttcacttc
    18781 actcatctgc gattttggca agtatctgca aggggtgcgg ggatatgacg tgatcttgta
    18841 tttttcgtag agggtttgtg caattttgag cactttggaa gccgataggc caagtttttt
    18901 accggcttct ttttgcaaag ccgaaaggct gtacggtaac gggggcgggg tgcttttacg
    18961 ctctttattt gaggtaataa ccttgcctgt agatttctca agctgtttga caagtgcctt
    19021 tacagcctca aggttggtac agtatccgtc agtattacgc cagttttcag gaacctgcca
    19081 cagtgtagta aacactgact tgtctgctgt ctcaaaatct actgaaaggg tgtagtagtt
    19141 gtaaggcacg aaagtatcga tctctttatc cctttggact acaagcttta aagtcggtgt
    19201 ttgaatccgt ccgatcgaca aaatatgttc tcggccatat ccggcagaat aagcagctgt
    19261 gtaggcacga ctcaggttca tacctaccag ccagtcggcg cggccgcgcg ccaaaccggc
    19321 ctgatatagg ttttctttat ccttacccgg cattattgaa gataaggctt tttttaccga
    19381 ttgggtatct aggccactcg tccaaaatcg ggaaatcttt ttacggtagc cacagtagtc
    19441 caatatttca cgggcaataa cttccccttc acggtctgca tcagtagcaa tcactacttc
    19501 atctgctttg gccaacaagc ttttaatgac tttaaactgc ttcgatacct tcggcggtac
    19561 ctgcatctgc caaacttcag ggagaatggg caatgcggag atatttccaa agtcagcata
    19621 ttgttcgcca taagcatctg gcgcggcctg tgtcaggata tgaccaaatg cccaggtaac
    19681 aatagtgtct ccattctgaa aataaccatc atgcctcccg ccaagaacac caagcacttt
    19741 accgatgtcc ttagcttgag atggtttttc acataaaaac aaacgcattt taaaatccca
    19801 agctttgctt cagctgcaaa acaagccaag atgcgatagt caaaaaagcc caaattgcca
    19861 aagcaattaa gccaccataa ataagggtaa caatgaacat atccttaaag tcagcccata
    19921 atgcagcaag ccaacagttc atttcacgaa aaaaggaagt attcttcgtg gccatatatt
    19981 tacaatggga tgtcatcatc agcgtcttca acgtgattat tgtcctgaga ctgctcacgt
    20041 ttaggacgca acttgatagc ttctacacgc tcagttggaa caatgaatac atcttgaaca
    20101 cgcaatttac gaacgcgaac ttcaataccg tcacgattgg tataagactc gtaacgttct
    20161 tcaccggtaa cgaaaaccgg cataccgacc tgtaatattt tatggagatg ggcagcatca
    20221 cgattccagt attcgcaaga aatccactcc gttgaaacca cttctgattt accttcggca
    20281 ttgcgtttga aattaggtga ggcaactgaa aaattcagta cgattgaggc ttcatcgttt
    20341 ttagggcttg ctacaggacg cagattaaat tcagcaccga gattgccacg aacaaaaagt
    20401 tgaactgaca tatttttctc caaaaaaagg atttaaaaat atgacagaaa agacagggta
    20461 aaataaccca atatgagatt aagggttcta ttatcataac taaataaatt cctcttgtca
    20521 actagcaact attacaaatt aaaccttatg ctatatctag ctaaaatgcc cacg
//
LOCUS       DAXDXW010000035        20461 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXDXW010000035 DAXDXW010000000
VERSION     DAXDXW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20461
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.036"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            150..2177
                     /gene="uvrB"
                     /locus_tag="ACFB64_001101"
     CDS             150..2177
                     /gene="uvrB"
                     /locus_tag="ACFB64_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC1209471.1"
                     /translation="MEVIRYPNSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDALRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAVDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKENLLFGAE"
     gene            complement(2355..3113)
                     /gene="cah"
                     /locus_tag="ACFB64_001102"
     CDS             complement(2355..3113)
                     /gene="cah"
                     /locus_tag="ACFB64_001102"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HFC1209472.1"
                     /translation="MPRFPRTLPRLTAVLLLACTAFSAAAHGNHTHWGYTGHDSPESW
                     GNLSEEFRLCSTGKNQSPVNITETVSGKLPAIKVNYKPSMVDVENNGHTIQVNYPEGG
                     NTLTVNGRTYTLKQFHFHVPSENQIKGRTFPMEAHFVHLDENKQPLVLAVLYEAGKTN
                     GRLSSIWNVMPMTAGKVKLNQPFDASTLLPKRLKYYRFAGSLTTPPCTEGVSWLVLKT
                     YDHIDQAQAEKFTRAVGSENNRPVQPLNARVVIE"
     gene            complement(3522..3869)
                     /locus_tag="ACFB64_001103"
     CDS             complement(3522..3869)
                     /locus_tag="ACFB64_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209473.1"
                     /translation="MTYLKVIAISIVLYILLLQINLKMLEKRIDFLVENIDKYYQQYG
                     SYPNNFDFISTKTDFTTESYCDFWDKNIAGYGNCYFVKNDKDYTILVMGFSSKILFSS
                     HNKIKEFNSNKYD"
     gene            complement(4193..4909)
                     /gene="trmB"
                     /locus_tag="ACFB64_001104"
     CDS             complement(4193..4909)
                     /gene="trmB"
                     /locus_tag="ACFB64_001104"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC1209474.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGMASAEIARRLPETDFLAIDVHGPGVG
                     NLLKLINENHLENIRVMRHDAVEVVKNMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(5031..6435)
                     /locus_tag="ACFB64_001105"
                     /pseudo
     CDS             complement(5031..6435)
                     /locus_tag="ACFB64_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(6552..8438)
                     /gene="uvrC"
                     /locus_tag="ACFB64_001106"
     CDS             complement(6552..8438)
                     /gene="uvrC"
                     /locus_tag="ACFB64_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC1209475.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVRHIETTITRSEAEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKNPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYCDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIIQSDQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAITQRHLQQSNQ
                     QHRIDELAKILGMNSDGINRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKIQEAEANGESVKWPDVVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGELFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLGDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(8493..10670)
                     /locus_tag="ACFB64_001107"
     CDS             complement(8493..10670)
                     /locus_tag="ACFB64_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC1209476.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VTFDGSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLTINIPFLIG
                     MVGMMLKGLNWTRHDWMIPPVWQFVLASIVQLWLAIPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSSHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRNGEWKQLPIDQVQIGDLIRTNHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPTVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGMGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYYVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLEIPAAQNAQTVVGAGITAEVEGVGLVKS
                     GKAEFAELTLPKFSDGVWEIASAVTVSVNGKPIGAFALSDALKADTAEAIGRLKKHNI
                     DVYIMSGDNQSTVEYVAKQLGIAHAFGNMSPCDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALLISQATLENIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLGNALRLKWVKID"
     gene            10809..11759
                     /gene="trxB"
                     /locus_tag="ACFB64_001108"
     CDS             10809..11759
                     /gene="trxB"
                     /locus_tag="ACFB64_001108"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC1209477.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFALKGDMGEY
                     TCDALIVATGASAKYLGLSSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSDQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            11915..12283
                     /gene="rpsF"
                     /locus_tag="ACFB64_001109"
     CDS             11915..12283
                     /gene="rpsF"
                     /locus_tag="ACFB64_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC1209478.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMITEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVGELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLVGAAEEAAAQ"
     gene            12284..12586
                     /gene="priB"
                     /locus_tag="ACFB64_001110"
     CDS             12284..12586
                     /gene="priB"
                     /locus_tag="ACFB64_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC1209479.1"
                     /translation="MGFTNLVSLAALIEKAFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIKEYKG"
     gene            12593..12823
                     /gene="rpsR"
                     /locus_tag="ACFB64_001111"
     CDS             12593..12823
                     /gene="rpsR"
                     /locus_tag="ACFB64_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC1209480.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            12840..13292
                     /gene="rplI"
                     /locus_tag="ACFB64_001112"
     CDS             12840..13292
                     /gene="rplI"
                     /locus_tag="ACFB64_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC1209481.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARARQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVIAAAE"
     gene            complement(13915..14721)
                     /locus_tag="ACFB64_001113"
     CDS             complement(13915..14721)
                     /locus_tag="ACFB64_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC1209482.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQTVDWKTIFAMMPGMIFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGRQTASRPLPGLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLVNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKESFGIMLLLIAGKMLYNLL"
     gene            complement(14722..15468)
                     /gene="pssA"
                     /locus_tag="ACFB64_001114"
     CDS             complement(14722..15468)
                     /gene="pssA"
                     /locus_tag="ACFB64_001114"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC1209483.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            16051..16533
                     /locus_tag="ACFB64_001115"
     CDS             16051..16533
                     /locus_tag="ACFB64_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC1209484.1"
                     /translation="MMKKILAVSALCLMTAAAQAADTYGYLAVWQNPQDANDVLQVKT
                     TKEDSAKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVSLAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGAVRSLIQHLK
                     "
     gene            complement(16580..17791)
                     /locus_tag="ACFB64_001116"
     CDS             complement(16580..17791)
                     /locus_tag="ACFB64_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC1209485.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVTGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTTSIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGIKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFVGVGLCAVLAIV
                     LNSLLPDPKES"
     gene            18013..20451
                     /locus_tag="ACFB64_001117"
     CDS             18013..20451
                     /locus_tag="ACFB64_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC1209486.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIADALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDA
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALRKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQPSLFEDNEVQQNGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPEEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEQAKAAGKPLLNPFSLNLDEPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGTFVSDGEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGIVSPSD
                     LNGSRKILAHKDHL"
BASE COUNT         4830 a         5158 c         5586 g         4887 t
ORIGIN      
        1 accggtttaa atttaatcta ctatacttgg gtagcgattg ggtttcagac ggcatacatt
       61 caggatttca ccgccttata ccgtctgaaa gacgtttctg acccatccct actacacgct
      121 ttgtccgcaa acgtatcaca ggcaagatta tggaagtcat ccgataccca aattccccct
      181 tcaaactcca ccaacccttc ccgcccgcag gcgaccagcc caccgccatt gcgggcctgc
      241 tcgaagggct ttcagacggc cttgcctacc aaaccctgct cggcgtaacc ggttcgggca
      301 aaacctacac gatggcgaac gtcatcgcgc aaagcggcag acccgccatc attatggcgc
      361 acaacaaaac ccttgccgcc cagctttatg ccgaaatgcg cgagtttttc cccgaaaacg
      421 cagtggaata tttcgtgtcg tattacgact attaccagcc cgaagcctat gtgcccagcc
      481 gcgatttgtt catcgaaaaa gacagcgcga tcaacgaaca catcgagcag atgcgccttt
      541 ccgccaccaa aaacctgatg acgcgcgacg acgtgattat cgtcgccacc gtgtccgcca
      601 tttacggtat cggcgacccg accgagtatc aacaaatggt gctgtccgta aaagaaggcg
      661 acaccatcga gcagcgcgac atcatcgcca cgctcgtttc catgcagtac gaacgcggcg
      721 atttggactt caaacgcggc agcttccgcg tgcgcggcga cgtgattgac gtgtaccccg
      781 ccgaaagctc cgaaaacgcc ttgcgtatca gcctgtttga tgacgaaatc gaccgcctcg
      841 atatgttcga cccgctttca ggcagcctgc accagcgtgt cggacgctac accgtcttcc
      901 cgtccagcca ctacgttacc ccgcgcgaca ccgtattgcg cgcctgcgaa tccatcaaag
      961 aagaattgcg cgaacgcatc gaatttttcg ctcgcgaaca acgccccgtc gagcaacaac
     1021 gcatcgaaca gcgcacccgt ttcgacctcg aaatgctcta cgaaatgggc ttctgcaaag
     1081 gcattgaaaa ctactcccgc cacttctccg gtaaaaaaga aggcgaaccg ccgcccacgc
     1141 tgatggacta cctgcccgac aacgccatca tgttcatcga cgaaagccac gttaccgtta
     1201 cccaaatcgg cggcatgtac aaaggcgacg cattgcgcaa gcaaaacctc gtggactacg
     1261 gcttccgcct gccttccgcc cgcgacaacc gcccgctcaa attccacgaa tttgaaaaag
     1321 tcatgccgca aaccgtcttc gtttccgcca cccccgcgaa atacgaagaa gaacacgccg
     1381 gacaagtcgt cgaacaagtc gtccgcccca cagggctggt cgacccccaa atcatcatcc
     1441 gccccgtcgc cacccaagtc gacgatttaa tgagcgaaat caacgaccgt attcaaaaag
     1501 gcgaacgtgt gctcgttacc acccttacca aacgcatggc ggagcaactc accgactatt
     1561 acagcgaact cggcatcaaa gtgcgctacc tgcacagcga catcgacacc gtcgaacgcg
     1621 ttgaaatcat tagagatttg cggctcggcc tgtttgacgt actcgttggc atcaacctgt
     1681 tgcgcgaagg cctcgacatc cccgaagtct ccctcgtcgc catcctcgat gccgacaaag
     1741 aaggcttcct gcgctcccac cgcagcctga ttcaaaccat aggccgcgcc gcgcgcaacg
     1801 tgaacggcgt cgccatcctg tacgccgaca aaattaccga ctccatgaaa gccgccgtcg
     1861 acgaaaccga acgccgccgc gaaaaacaga ttaaattcaa cgaagaacac ggtatcgtgc
     1921 cgcagcagat taaaaaacag gtcaaagaca tcatcgacgg cgtgtaccac gaagaagaca
     1981 gtggcaaagg ccgtagacaa ggcaaaaaca aggtcaaagt cggcgaaatc cacaacgaag
     2041 aagacgcgat taaagaaatc gccaaactgg aaaaagccat gcagcaggcg gctagggatt
     2101 tacagtttga agaagctgct gtgttgaggg ataggattag gaatattaaa gagaatttgt
     2161 tgtttggggc ggagtgattt tgaaatagta atcgcagcct gaatacatag cgtgtgcgcc
     2221 actggcgcgc gctcattgtg ttatcaggaa tccttgaaat catgtacgca gcttgcatca
     2281 cacatcctag ctatacataa acaacaaggc cgtctgaatt ttcagacggc cttttccgtt
     2341 tacaacttta ctacttattc aataactaca cgtgcattca gaggctgtac ggggcggttg
     2401 ttttccgaac cgacggcgcg ggtgaatttt tccgcttgcg cttggtcgat gtggtcataa
     2461 gttttcaaca ccaaccatga tacgccctct gtgcacggcg gcgtggtcag cgaaccggca
     2521 aagcggtagt atttcaaccg tttcggcagt agggtggatg cgtcgaacgg ttggttgagt
     2581 ttcacttttc ctgcggtcat cggcatgacg ttccagatgg aagacaggcg gccgttggtt
     2641 ttgccggctt catacagcac ggctaatact aaaggctgtt tgttttcgtc taagtggacg
     2701 aagtgagctt ccatcgggaa agtgcggcct ttgatttggt tttcgctcgg cacgtggaag
     2761 tggaactgtt tcagggtata ggtgcggccg ttcacggtca gggtattgcc gccttcggga
     2821 taattgacct gaatggtgtg gccgttgttt tccacgtcaa ccatactcgg tttgtaattg
     2881 actttgatgg cgggcagttt gccggaaacg gtttcggtaa tgtttaccgg agattggttt
     2941 ttgccggtgg agcacaaacg gaattcttct gacagattgc cccagctttc gggagagtcg
     3001 tgtccggtat agccccaatg ggtgtgattg ccgtgtgcgg cggcggagaa agcggtacag
     3061 gccaacagca ggacagcggt caggcggggt aaagtgcggg ggaaacgagg cattgtaaga
     3121 tcctttctat taaggagtag aaaaaaggtt gcagtgtgta tgtaaatatt ttatttttta
     3181 ttaaatgata tctatattaa gtgcttaagt caaacttccc ggtctgaaaa ccattagagc
     3241 aatgaaatta aagtgaattt atttttctat aagtcctgct ttatttggat aaccatgagg
     3301 catggctacc tttatccgga aaaatattaa tagccgcaac aaagatattt gaatccgatt
     3361 caaatatcac cagcaagcgt agctcggata cttgtatccg acaaagcctt taacatttcc
     3421 atcgttgcaa tccattgcag caattaccaa aatgtcggat tcgagaatcc gatctacgga
     3481 aaactaaaga ttttatgcgg gctacggctt gcttgttgta tttaatcata tttatttgaa
     3541 ttgaattctt ttattttatt gtgagatgaa aatagtattt tcgatgagaa acccatgact
     3601 aaaatagtat agtctttatc attctttacg aagtaacaat taccatatcc tgcaatattt
     3661 ttatcccaaa aatcacaata tgattcagtg gtaaaatcag ttttagttga tataaaatca
     3721 aaattatttg ggtaagatcc atattgctgg taatatttat ctatattttc tactaagaaa
     3781 tcaatccttt tttctaacat tttaagatta atttgcaata ataaaatgta taaaactata
     3841 ctaattgcta ttacttttaa atatgtcatt ttattttcca gtaaatagaa gtttagttgt
     3901 tttgttaact atgcctgaat gtacacctaa gtcttctttt ggtaacgatg aataattagc
     3961 aagccgtagc ctgcatctcc gccaaccgcg tgccactatc tgcgggcgga ataaacttta
     4021 aaaccgatgt gcaggttaaa acctacaagg atgcgtgcgg tatacacgcg tttttgccgt
     4081 tcaaaccatt gtttcaaaac ttgtattgaa acatcgcccg ctgctttatc ttgaaagaat
     4141 gccgtctgta agcaaacatg cttcagacgg cattttttta tccggcggtc tgttatccga
     4201 tccgtttgaa taccaagtcc caaacgccgt gtccgaggcg tttgccgcgc gcttcgaatt
     4261 tggtttcggg gcggtagtcc ggagtggggg cgtagtctgc cgccgtattt tgcaggctgt
     4321 cgaagctgct gaggacttca agcatctgct gtgcatattc ttcccagtct gtcgccaggt
     4381 ggatatagcc gccggttttg agtttgggca gtagtttggc gatgaagggg gcttgtatca
     4441 gacggcgttt gtggtggcgt tttttgtgcc acgggtcggg gaagaatatg tggatgccgt
     4501 cgagcgagcc gtcttgcagc atatttttga caacttctac ggcatcgtgc cgcatcacgc
     4561 ggatgttttc caaatggttt tcgttgatga gtttgagcag gttgcctacg ccggggccgt
     4621 gtacgtcgat ggcgagaaag tcggtttcgg gcaggcggcg ggcgatttct gcgcttgcca
     4681 ttcccatacc gaagcctatt tcgaggattt taggtcggct gcttccgaaa cgggcatcaa
     4741 gatcggccgg tgttgcttgg tagtcgatgc cgaagctgtc ccataaggta tcgatggcgc
     4801 gttgctgtgc ggcggtcatg tggctttggc ggaggacgaa actgcggatg ctgcgggggt
     4861 gtccggcttg acggtcgttt ggtgtttggt tttcggcggt gtctgtcata atgcttctgt
     4921 gcgcttgtgc aatcaggatg ccgtctgaat gtgaaaggcc tgttcggacg gcgggaaggg
     4981 aaaggggcga ttatacctgt ttcgtttcgg cagggtaggt gtaactctgt tcagacggca
     5041 ttttgccact gttgcagcag ttcttgcagg acgctttttt gcccgtatcc gttgtttatg
     5101 gcttcttgca ggtagcggca ggctttttct ttgtccgcct ggcaccgtgt ccgctgtaat
     5161 ggatgcaggc gaggttgtag aacgccatac tgtctttttg cgcggcggct tgttcaaacc
     5221 atttgcgggc tttgcagtag tcggcggcta tgccctgtcc gaagtagtac atactgccca
     5281 gattggtttg ggctttgctg tgtccgagtt ctgctgcttc tgtgtagagt ttgagcgcgg
     5341 ctgcgtattc gggcgcgcat tgtaatccgt aatgcagggc ttgggctttt tgataaagct
     5401 gttctgccct ttgccgccta atggcggaat ctttgatgcc gtcatattgc tcgggatggt
     5461 ttaacgcgct gtcggatatg agtttctgat aggcggtaag gctgccggct tcggctgcct
     5521 gccggtaata atgccgggct ttttccgtat cgagggctat gcccaaaccg tagcggcaga
     5581 tgtcgcccgg tatccgcaaa ccttcgggat ggcgttcggc ggcggctgct tcggcatgat
     5641 gcagggcaag tttgtggttt ctttccagat acttgccttg caggtagatg tcggccagtg
     5701 cggcatgggc atcggggtca ttttcctgtg ccgcctgttg gaaccggtaa acggcttcat
     5761 cgggagccgt aggtaggatg agttcggcaa gcggggtgta ggcgggaatg tatccttgtt
     5821 gcgcggcggc gcgcaaatga ttgacggcct gtgccgtgtc ggctttcgtc ccttgtccga
     5881 aacggtattg cagcccgagt tgccaatgtg cttccggcaa gccttgtgcc gccgctttgc
     5941 ggtagagcag gtgcgccgtt tcaaaatccg gcggcgtgcc gtgccggcag tattgggcga
     6001 gcaggtattg cccgtgggga ttgttgtttt ttgcgcttgt tttgcccatt ccagggcttc
     6061 ttcaaaatgt cgggacgcgt acagaaggta catcaggcgg acttgcgctt cgggcaggcc
     6121 tgcttctgcc agaggcctgt aaagcggtat cagggtttcc gggggcgcgc cttccaattc
     6181 ttggatatat gccaaattgt aacgcgcata gggatgcccc tgttcggcgg cgatgcggag
     6241 catttccata ccttgttcgc ggtcttggtg cgggctgtcc gcgttcatca ggtgttttgc
     6301 caactcgaat gtggcggcgg gatgatctgc cgaggccgct tctgtcagca gtcggcggac
     6361 ttcggtaaag ttataatatt gtttttgaga caggcgttcc aaggcctgcc gatagggtaa
     6421 gtcgggattg ttcatatcag atcgttttcc gttttgtcga atcatcgtta ttcgtcatga
     6481 agttaggttt atatcggtat gccggcctgc tgtatcatat ttttacaggc ggtttttgct
     6541 ttggcggcat actaatgcag atgattataa atggtttccg ccaatgcctt gctgatgcct
     6601 tccacttttt ccaagtcctc gcggctggcg gcaatcacgc cgcgcagtcc gccgaagcgg
     6661 gtaagcaggg cttggcggcg tttgctgcct acaccgggaa tgtcgccgag agacgaggta
     6721 acgcgtgctt tgtcgcgttt tttacggtgg cctgtgatgg caaagcggtg tgattcatcg
     6781 cgtacggttt gcaataaatg caaggccggg ctattaggag gcaggcggaa gagttcgccg
     6841 gtaaaaggca gtatgagttc ttccatgccg gctttgcgtt cggggccttt ggcaataccg
     6901 actaaaggga tgtgcagccc gagttcttcc catacggata cggctacgcc gatttgccct
     6961 ttaccgccgt caatcaacac gacatccggc catttgacgc tttcgccgtt ggcttcggct
     7021 tcctgtattt tgccataacg gcgcgtcaac acttcgcgca tggcggcgta gtcgtcgccg
     7081 ggtttggcgg tggtgatgtt gtagcggcgg tattgcgagg gttggatgtt ttgttcgtcg
     7141 tacacgacgc aggaggcgat ggtggcttcg ccttgcgtgt ggctgatatc gaaacattca
     7201 aggcggttga ttccgtctga attcatgccg aggattttgg caagttcgtc gatgcggtgt
     7261 tgttggttgc tttgttgcag gtggcgttgg gtaatcgcca tttgtgcgtt ttgttccgcc
     7321 attttcaacc aaactttgcg ttcgcctatg gttttggtaa cgaactgcat ctgcttgccg
     7381 tgttctcctt ccaaagcctc tttcaacgca tcgggaacgg gaaagttgct gatgatgatg
     7441 tcgggtttgc ttttgcccag atagtgttgg gcgacgaagg cttcggcgta atcttgaccg
     7501 tttggttcgg ggtcgttttt ggtgtcggga aagaagcttt tgtcgccgac gtgccgtccg
     7561 ccgcggatgc tgacccagtg tacgcagaca aggccgtctg aaacggcgag cgcgagcagg
     7621 tcgatatcgt tggggttgtt cgggtttttg ctgtcgatga actggtcgct ctgtataatg
     7681 ccgagtgctt ggatttggtc gcgataacgg gcggcttctt caaattgcag attcgcggcg
     7741 gcggtctgca ttttgtgttg cagggtgcgg gtcagttcgt cggttttgcc gttgaggaaa
     7801 gtggcggctt gacgcacgct gtcgcagtag tcttcttcgc tgatgtgtcc gacgcagggc
     7861 gcggtgcagc gtttgatttg gtacagtagg caagggcggt cgcggtgttc gaacacgctg
     7921 tcttcgcagg tacgcagcat gaagactttt tgcaacactt gaatgctgtc gcgcacggcg
     7981 ttgctgttcg gatacgggcc gaagtattgg ttgggatttt tcagcgtgcc gcggtaatac
     8041 gccatttgcg gatattgatg gccgctgagc atcaaataag gatagctttt gtcgtcgcga
     8101 aaaaggatat tgtatttcgg cgacaaggct ttgatgaagt tgttttcgag aatcagggct
     8161 tcggcttcgg aacgcgtgat ggtggtttcg atgtggcgaa cctgtttcac catcaacgcg
     8221 atgcgcggcg aatggtcgtt tttttggaaa tagccggaca cgcgccgctt gaggttgacc
     8281 gctttgccga cgtataagac gttgccgcct tcgtcaaaaa aacggtacac gcccggcagc
     8341 ttgggcaggt ttttgaggaa gagcgggatg tcgaattgtt ccggagaacg gttttctgta
     8401 ttcatatcaa tcatttgaaa aacggcttca gacggcatat cggcagcggc agccttatcc
     8461 gttcggaaaa gctgtcgggc ggttatatgc ggtcaatcga tttttaccca tttcaggcgc
     8521 agggcattgc ccaataccga aaccgagctt gccgccattg ccgcgcctgc tatgacggga
     8581 tttaaaaagc cgagcgcggc gagcggaatg cccaatatat tgtagaagaa ggcgaaaaat
     8641 aggttttgct tgatgttttc caacgttgcc tgcgatatca gcagggcatc ggcgagctga
     8701 ttgaccgaat gctgcatcag cgtggcggag gcggtgtgtt cggcaacgtc cgcaccgcct
     8761 ttcatggcga agctgacgtt ggcggcggca agcgcgggcg cgtcgttgat gccgtcgccg
     8821 accatcgcca cggttttgcc ggcggctttg agtttctgca cttcggcggc tttgtcgcac
     8881 ggactcatat taccgaaggc gtgtgcgatg cccagttgtt tggcgacgta ttcgaccgta
     8941 ctttggttat cgccgctcat aatatagaca tcaatattgt gttttttcag acggcctatg
     9001 gcttcggcgg tatcggcttt caacgcgtcg gagagtgcga atgcgccgat cggtttgccg
     9061 tttacagata cggtaaccgc actggcgatt tcccaaacgc cgtctgaaaa cttcggcaag
     9121 gtcagttcgg caaattcggc tttgcctgat ttcaccaaac ccacgccttc cacttcggcg
     9181 gtaatgcctg ctccgacaac ggtttgcgca ttttgtgcag cgggaatctc caaaccgcgc
     9241 gcttgtgcgg cggagacgat ggcgcgggcg agcgggtggg cggcgttttg ctcgacggcg
     9301 gcggcgatgc ggtacaaagc gtcttcgtca aagccgctgt cgggaacgta ataaacggcg
     9361 gcaacctgcg gcctgccttc ggtcagcgta ccggttttgt ccaatacgac ggcatcgacg
     9421 tgggctgctt cctccattgc cgccgcgtct ttaaaccaaa tgccgtgttt caccgctttg
     9481 cccatgccga ccataatcgc ggcaggggtc gccagaccga gcgcgcacgg gcaggcaatc
     9541 accaaaacgg caacggcgtg catcagtgcg accgtccaat cgcccttaat cagccaagta
     9601 acgataaaag tcagaagcgc gatgcccacg acagttggca caaataccgc cgccgcttta
     9661 tcggccacgc gcgcaatcgg tgctttactg ccttgtgctt cagagagcgc gttcatcatg
     9721 tcgccgagca gggtttggct gccgagctgc gcggcgcggt acaccacgct gccttcggtc
     9781 atcagcgcgc ccgccaacac tttgccgccc gcctttttct cttcgggatt ggattcgccg
     9841 gtaaggtggc tttcgtccgc ccaaccgctg ccgctttcga taatgccgtc ggcagcgatg
     9901 cgttcgccgt ggttggtgcg gataaggtcg ccgatttgca cttggtcgat gggcagttgt
     9961 ttccattcgc cgttgcgttg cacgttgact tgggtcggcg tgagttttag cagtaagccc
    10021 aagctgttca ggctggattt tttggtgcgg tgttccaaaa acttacccag cgacacaaaa
    10081 ccgatcacca tcacgcccgc ttcaaaatac acatgcgcca taccgtgcgc cgcatgcgaa
    10141 ctgaaaaaca gcatataaac ggaatacagg taaatcgaca ccgtgccgat ggtaacgagt
    10201 acgtccatat tcgccagccc gcctttaatg cttgcccacg cgcttttgta aaacgggatt
    10261 gccagccaaa gttgcactat gcttgccagt acaaactgcc atacaggcgg aatcatccaa
    10321 tcgtgccgtg tccaattcag cccttttagc atcatcccta ccataccgat aaggaacggg
    10381 atattgatgg tcagcaaaag ccacaaccgc cagccgatat ggtgttctgc ttcaggttgc
    10441 ggcaatgtat cttccgtttt ttccttcgcg ccgtaaccgg ttttctcaat gattttggca
    10501 atgtcggcaa ccgaggtttt gctgccgtca aacgtaacct gcgcttcctc actggcaaag
    10561 ttcactcccg ccgattcgac aaaatctttt ttgttcaaca ctttttcaat gcgcgaagca
    10621 catgcctgac aggtcatccc ctcgatttgg aaacggattt tttgttgcat aagtttgatt
    10681 cccgttttga tgttcagacg gcagtcaagg ctgcgggatg ccgtctgaac gccggtatgg
    10741 tcaaaatttc tcaaacgcgc cgataattcg ggtagaatcc gaaaacttga cacagcaaag
    10801 gaaaaacaat gtcccaacac cgcaaactga ttattttggg ttccggcccc gccggataca
    10861 ccgccgccgt ctatgccgca cgtgccaatt taaaccccgt cattattaca ggtatcgcgc
    10921 aaggcgggca actgatgaca acgactgaag tggacaactg gcctgccgat gccgacggcg
    10981 tgcaagggcc ggaattgatg gcgcggtttc tcgcccacgc cgaacgtttc ggaacggaaa
    11041 tcatttttga ccaaatcaac gccgtcgatc tgcaaaaacg cccgttcgca ctcaaaggcg
    11101 atatgggcga gtacacttgc gatgccctga ttgtcgccac cggcgcgtcc gccaaatacc
    11161 ttggtttgtc gagtgaggaa gcgtttgcag ggaaaggcgt ttccgcctgc gccacctgcg
    11221 acggtttctt ctacaaaaac caagatgttg ccgtagtcgg cggcggcaat acggcagttg
    11281 aggaggcact ctaccttgcc aatatcgcca aaaccgttac gctgattcac cgccgcagcg
    11341 agttccgtgc tgaaaaaatc atgattgaca aactgatgaa acgcgtggaa gagggcaaaa
    11401 tcatcctcaa gctggaaagc aacctgcaag aagtactggg cgacgatcgg ggcgtaaatg
    11461 gcgcattatt aaaaaacaac gacggttctg atcaacaaat tgccgtcagc ggcatcttta
    11521 tcgccatcgg gcacaagccg aataccgata ttttcaaagg gcagttggaa atggacgaag
    11581 ccggctacct gaaaacaaaa ggcggcacgg cggacaatgt cggtgcaacc aatatcgaag
    11641 gcgtatgggc ggcgggcgac gtaaaagacc atacctaccg tcaggcaatt accagcgcgg
    11701 cctccggctg tcaggcggct ttggacgcgg aacgttggct gggcagccaa aacatttgaa
    11761 tgccgtctga aaccgaaatt ttcggcggaa tgcaagtttt tcgtaccaaa tccttgattc
    11821 cgtcggcaaa ccccaatata atgcctgcta tttgaacctt gccttctgct ttcgcatggc
    11881 agaaggctgt ttttaccatc cataaggaga taacatgcgt cattacgaga tcgtgtttat
    11941 cgttcatcct gatcaaagcg agcaagtgcc cgctatggtt gaacgttaca aaaccatgat
    12001 taccgaggca aacggtaaga ttcaccgttt ggaagattgg ggccgccgcc aactggctta
    12061 cccgattaac aaaatccata aagcacatta tgttttgatg aacatcgaaa ccactcccga
    12121 agttgttggg gagttggaaa ccgcgttccg tttcaatgat gcggtattgc gtcatctgac
    12181 catcaaaacc aaacacgccg ttaccgaagc ctcccctatg ctgggcggcg aaaaggctaa
    12241 gaacctgttg gtcggcgcgg ctgaagaagc ggctgcccaa taattgggat tcactaatct
    12301 tgtttcgctt gccgcgctga ttgaaaaggc tttccctatt cgatatacgc ctgccggaat
    12361 ccctgtttta gatattattt taaagcacga atcgtggcag gaggaaaatg ggcagcaatg
    12421 ccttgtccaa ttggaaatcc ccgcgcggat tttgggtagg caggcggaag agtggcagta
    12481 tcggcaaggt gtatatgttc acgtcgaagg ttttttagct caaaaaagca gacgttccct
    12541 gatgccgatg ctcaggatac aaaatattaa agaatataaa ggttaaacga caatggctcg
    12601 tcaatcattc aaacgtagaa aattctgccg tttcacggct gaaaaaatcc aagaagtcga
    12661 ttacaaacaa gttgatttgc tgaaagactt tatctctgaa aacggtaaaa tcattcctgc
    12721 ccgcatcaca ggaacgaagg cattctacca acgccaattg gctgttgccg taaaacgcgc
    12781 gcgcttcttg gcactcctgc cttataccga ccaacacaaa taattttgga gtttaaatca
    12841 tgcaaattat tctgttagaa aaaatcggcg gtttgggtaa tttgggcgac atcgttaccg
    12901 taaaaaacgg ttatgcccgc aacttcttga ttcctgctgg taaggcaaaa cgtgctactg
    12961 aagcaaacat gaaagagttt gaagcacgcc gcgccgaatt ggaagccaaa caggccgaaa
    13021 ttttggcaga tgcccgagcc cgtcaggaaa aactggacgg tcaaaccgtt accgttgcac
    13081 agaaagccgg tgtggacggc cgcctgttcg gttccgttac caatgccgac attgctgctg
    13141 caatcgttgc tgccggcatc gaagccgtga aagcaaatgt acgtctgccg aacggtcctc
    13201 tgaaagccgt tggagagtac gaagtggaag tggctttaca cactgatgcc gttgctaaaa
    13261 ttactgttgc cgtcattgcc gcagccgagt aattgaaatt atcagatgcc gtctgaaacc
    13321 ctgccgatta tcagaatgtg gtttcagacg gcatttgttt tgttttccgt atattctgca
    13381 aaaatcggga cggttgtttt ttatttgcct gaggtctttg cagaaatggt ttgttaacga
    13441 aatttgatgc ataaaatgcg ccaaaaaata ttggctgtgt tttaatatag tgttgacaag
    13501 cgtacctggt ttcaatatat tcaataggat aggtctcttc ccaaccgaaa aaataattgt
    13561 cttttctctt gtaaatagtt gaaatagatt gtcaaaatac atcctaaaaa atttaataac
    13621 ttatatgatt gcaaaggttg cggcttgtat gccggtcatt atggcggaaa taccgtttgg
    13681 gaggcagtcg catcgcatgg agtcggcacg gcaggcgatg cgccgatgcc ttcgggcgtg
    13741 aaggcaggga aatcggcagt ttggaaaaag gcaatggaat ccgcaggtag gtgtcgattg
    13801 ggtggtgaaa ggcaataagt cgggcgcacg ctcagactgc ctatcccctt gtcggaacgg
    13861 gaggtagggc agctttgtcc tgactaattg cttgtgcaaa cggtaagaag gtttttaaag
    13921 caggttgtac agcatttttc cggcaatcaa aagcaacata atgccgaagg attctttgag
    13981 tttggcagaa gaaagtttgt gggcggtttt gacaccgagc ggggcaaagg caatagttgc
    14041 cgcgctgagg acggcgacgg cgggcaggta aaggaagccc agcgaccctt cgggcaatcc
    14101 tgcaatattc agaccgttga ccagatacga tattgcgccg gagagtgcaa tcggccaggc
    14161 aaggccggat gatgtgccga tggctttatg ggcggggaag ccgcagtgga ttaagaaggg
    14221 gacggaaagt gaaccgccgc ctatgccgac ccagctcgac attgcgccga acagtgtgga
    14281 aaccgcagtc agtccgggca gcccgggcag cgggcgggat gccgtctgac gaccggtatg
    14341 cagtgttttg aatgcgacgg cggttaaaaa caggatgaag aaaatttgaa gcccgaacgc
    14401 ggggatatat tttgcggaga gtgcgccagc gaatacgccg aatatcatac ccggcatcat
    14461 cgcaaatatg gttttccagt cgaccgtctg ttttttgtgc tgccccaaca tactggaaaa
    14521 ggcggtgaag accatgacgg cgaaggatgt gccgacggcg aggtgttgcg cgtaaggatg
    14581 ttgtgccaaa ccctgcaaat caagcaccca taaaacgaca gggacaatca gcgtaccgcc
    14641 gcctacaccg aacaggccgg caataaaacc tgccgcactg cctacggcaa gcaggattaa
    14701 gataatgtcc caatgccaca tttaatccgc ctttctgtat tttttccaaa atcggcgtgc
    14761 cgccataatg tagccggaca ggctgtatcc gagaaagaac aggaaaagga cgagcgacgg
    14821 ttcccaagtg accagaagca gcagtaagac ggcaagcagc attccgacaa aggggacttg
    14881 tctgcggatg ttgatttctt taaaactcca aaaagggatt tggacaatca tcgacaggcc
    14941 ggcaaacagt gtgatgccta atgcccacca gtggacggcg gggaattttt cgacgctgtg
    15001 gttgacccaa atcagcccga caatcagcgc ggcggcagtc ggactgggta cgccgataaa
    15061 ccagcgtttg tccaccttgc cgatgagcgt gttgaacagc gcgaggcgta gggcggcgca
    15121 ggcgcagtag atgaaggcga cggaataacc gattttgccg aactgccaaa gctgccattt
    15181 gtaggcaata agcgcggggg caacgccgaa gctgaccatg tcggcaaggc tgtcgagctg
    15241 ctccccgaac gcgctttggc tgttggtcag ccgcgccacg cgcccgtcca taccgtccag
    15301 cagcatagag atgaataccg cgatggcggc ggtttcataa cgtccgtgca tggattgggt
    15361 aatggcgtaa aacgcggaaa acagcgcggc gatggtaaag gaattgggca gcaggtagat
    15421 actgttttga cgcagggagc gttgcggggt gttggtcggg gtttccatag ctgaagtgtc
    15481 tcacggacgt ggagaatcga taggggatta taacccaaac cattatcgaa caatacgccc
    15541 tttaggatga agccctcgga ggcggtttgt ttttctgatg gcggatgctt tgcggggcgg
    15601 tttgactctg cctgccaccg ggtgtgttgt ttcggatttt gccggatgga tattgttgcc
    15661 gggcatttct gcgcttgccg gaaggtatcg gagggaaggc atcggaatat gcggcgcgcc
    15721 gttcaaaccg gggcggtatt tccctatttt ttggggggtg caggtttcaa caaattgaaa
    15781 gatatttttt gcatttccga ccgtatgctg attttgaagc gggataaatt ataggacaag
    15841 gtttttcgcc cgccgcaaag gttgcccgca atgccgtctg aaaacaacgg cttgccgcta
    15901 aggcggcaat atgaggattt gcgctgccga aaccggtttg ctacaacccg gcagtgaaat
    15961 ctgcagggag ggataggccg ataaggcggg acttatcttg ttttttgttt ataatgcaac
    16021 agaatttgga ggaatcgtcg ggaatttatc atgatgaaaa agatactggc agtatcggca
    16081 ctatgcctga tgactgcggc ggcacaggct gccgatactt acggctatct cgccgtttgg
    16141 cagaatccgc aggatgcaaa cgatgttttg caggttaaaa ccacaaaaga agattcggcg
    16201 aaaagcgaag cgtttgccga gttggaagcc ttttgcaaag gtcaggacac gcttgcgggc
    16261 attgccgaag acgagccgac cggatgccgg tcggtcgtgt cgctgaacaa tacctgtgtc
    16321 tcgctggcat acccgaaagc cttgggcgcg atgcgcgttg aaaacgccgt cgtgattact
    16381 tctccgcgtt ttacgagcgt tcatcaggtc gcactcaacc agtgcataaa aaaatacggc
    16441 gcacagggac aatgcggctt ggaaacagtg tattgcacgt catcttctta ttacggcggg
    16501 gctgttcgct ccttaatcca acacctgaaa taaaaaatgc cgtctgaagg atgttcggac
    16561 ggcatttcca tatcgacggt caggattctt tcggatcggg cagcaggctg ttcaacacaa
    16621 tggcgagtac ggcgcacaag cccacgccga caaagctgaa gctgcccaat ttgagtgtca
    16681 taccgccgat gcccgtggtc agtaccgagc tgacgatgac caggtttttc ggcagcatca
    16741 aatcgacttt ggcatcaatc agggttttga tgcctaaaga agcaatcgtg ccgaacagca
    16801 gcagcataat gccgcccatc acgggcatcg gaatggaagc caaaaacgca ttgaatttgc
    16861 cgaaaaacgc catgcagacg gcaaaaaccg ccgcccaagt catgatgacg gggttgctgt
    16921 ttttggtaat catcaccgca cccgttactt cgccgtaggt tgtaaccggc gggccgccga
    16981 tcagacctgc aacgcacacg cccaagccgt cgcccgcaag ggttttgtcc aagcccgggt
    17041 cttttgtgta gtcttttccc gttacattgc cgattgccat gatgccgccg atgtgttcga
    17101 tggcgggggc gacggcaacg ggcagcataa acagtgccgc ctgccagttg acctgaggcg
    17161 tttcaaaatg gggaacggcg aaccagggcg cgtgtgcaat gcttgtcgtg tccaccagtc
    17221 ccatcagcag tgccaaaaca taacccgaag cgacaccgat caagatggga atcagcttca
    17281 tcatcctgct gccgaaaacc gatacgatgg cggtaacggc aaaggtaaag ccggaaagaa
    17341 tcagcgaatc ggtatagtcg atgacctgtt tgccgtcagc ctgacccatt gccatgctgc
    17401 ttgccgccac ggcaacagac agaccgatga ccatgatgac agggccggtg acgaccggcg
    17461 gcagcagttt gtgtaccgcc gccagtccgc gccaacggat cagcgcggca aacacaaaat
    17521 acataaagcc ggcggcaaac agcccgaaca tggtggaagg cagcccccat tcgccgacgg
    17581 agtagataat cggcgcgata aaggcaaacg aagaaccgag aaaaatcggt actttgcgtt
    17641 tggttgtgat ttggaacagc agcgttccca agcctgcgcc caaaagcgca agagccggat
    17701 tcagaccggt cagcagggga accagcacca ttgcgccgaa tgccacaaat aaaatctgcg
    17761 cgccggaaac ggcaagtttc agttggttca taccatcttc tttcaatcgg ttcaaacggc
    17821 acggattata ctggtttgcc gaggggctgc gtaaaaatac atacgtcggg caaagatatg
    17881 ccgtctgaaa ggcaacgtct gtctgttgca ggatgaaagc gggagaaatg gagtataatc
    17941 cgacactttc catattcaat ccgcaagccg tcccgaaccc ttatttcaga cggcattttc
    18001 cggtaatacg gcatgacaga aaaatcccat aaaaaaacag caaaaggcag ggcgggaagc
    18061 ccgtccccaa cgtccgcacg caacaagaaa gccgacaacg gcgcgcgggg caataaagtt
    18121 tccgagcggc tcaaggcggt caaagagctg cagaaaaccg aaaccaaaaa ggcgcgtccc
    18181 gaacatgtcg tcaacctgat tgccgacgcg ctgtggctga tgggtttggc ggcaaccctg
    18241 tatttggcga tttccctgat cagtttcgat atgggcgatc cgtcttggtc gcacagttca
    18301 ccggttgtgg aagatgccgc caattggggc ggactgttcg gcgcgtatgt tgccgatgtc
    18361 ggctattatc ttttcggctg gtcgttctgg tggtggatag cggctgcctg cgtcgtgctg
    18421 tataaaaatt tccgtctgca cgcaaaacag acggaaaacg aggcatacaa ccacaaaatc
    18481 gctgccgccg cgctgtttgt cctgacggtc ttcagccccg tcttggagta ttttgtgctg
    18541 ggcggaaaat atgccgactc cctgcctgtc ggagcaggcg gtatggtcgg catacgtgtc
    18601 ggcgcagtgt ttgcgtggct gttggggaaa tcgggcagcc tgctgattat cctggttgtt
    18661 ctgctgttgt cgttgtccct gctggtgcag atttcatggc tggaattttt gaacggtgcg
    18721 ggcagggcgg ttcaaaaccg cctgagtgcc ttatccggca aggtcatggc tttgagaaaa
    18781 cgccggccga ataccaaaac agacggtgtc gatacccaaa atacacggcg catggtgaaa
    18841 gaagccaaaa acatcactgc caaacccgtt gccttgcccg aaggcagcag cagcaaccgc
    18901 aaatccgtcg cggtttccgt tgcgccgccg cctaaaatcc aaccttcgct gtttgaagac
    18961 aatgaagtac agcagaacgg cgaataccac aagcctacat tgaacctatt gcggattccc
    19021 gacagcgagc cggtcagcat caatcccgcc gaattggagc gcactgccga actaatcgaa
    19081 tccaaactgg cagaattcgg catcggcgta caagtcgtat ccgcgacatc cggccccgtc
    19141 atcacgcgct acgaaatcga acccgcgcaa ggtgtcaaag gcagccaaat tgttgccctg
    19201 tcgaaagatt tggcacgctc tatgtcgctg caatccgtgc gtatcgtcga aaccattgcg
    19261 ggcaaaaaca cgatgggcat cgagttgccc aacgacaaac gccaagacgt gatgttgagc
    19321 gaaatcttgt cctcgcccgt gtttgccgaa gccaaatcca agctgaccgt cgcgctgggc
    19381 aaagacatcg ccggtacgcc cgtcgtcggc gacttggcga aaatgccgca ccttttggtc
    19441 gccggcatga ccggttcggg caaatccgtc ggcgtgaacg gcatgattat gtctatgctt
    19501 ttcaaagcta cgcccgaaga agtccgcttc attatgatag acccgaaaat gctcgagttg
    19561 agcatttacg acggcattcc gcacctactc tgtcccgtcg ttaccgatat gcgcgaagca
    19621 gggcaggcgt tgaactggtg cgtcgccgaa atggaaaaac gctaccgcct gctctcccac
    19681 gccggtgtac gcaatctgga aggcttcaac caaaaagtcg aacaagccaa agcggcaggc
    19741 aagccgctgc tcaatccgtt cagcctgaac ctcgacgaac ctgagccact ggaaaaactg
    19801 ccgatgatcg tggtcgttat cgacgaactt gccgacctga tgatgaccga acgcaaagct
    19861 gtcgagcagc aaatcgcccg cctcgcccaa aaagcgcgcg ccgccggcat acacatgatt
    19921 gtcgccaccc aacgcccaag cgtcgatgtc gtaaccggtc tgattaaagc caacatcccg
    19981 acgcgtatgg cgtttaccgt gcaaagcaaa atcgacagcc gtaccatcct cgaccaaatg
    20041 ggcgcggacg aactgctcaa atatggcgat tcgctgttcc tgcaaccggg cagtgccgaa
    20101 ccgacccgcc tgcaaggcac gtttgtttca gacggcgaag tgcatcaagt cgtcaactac
    20161 gtcaaatcgc aagccccagc cgactatatt gaaggtctgc tcagcggcga agccgcgctg
    20221 gaaactgcca atatcgttaa tccgaatgca gacagcgacg aattattcga tcaggcagtc
    20281 gcctatgttt tggaaagcaa aaaaacctcc atttcgtctt tgcagcggca gttgcgtatc
    20341 ggctataacc gcgcggcaaa cctgatggag gcactggaaa atgcgggtat cgtttctccc
    20401 tccgacctca acggcagccg taaaattttg gcgcacaagg accatttata gcccgtatcg
    20461 c
//
LOCUS       DAXDXW010000036        20237 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXDXW010000036 DAXDXW010000000
VERSION     DAXDXW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20237
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.037"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            19..665
                     /locus_tag="ACFB64_001118"
                     /pseudo
     CDS             19..665
                     /locus_tag="ACFB64_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            complement(737..2482)
                     /gene="recD"
                     /locus_tag="ACFB64_001119"
     CDS             complement(737..2482)
                     /gene="recD"
                     /locus_tag="ACFB64_001119"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC1209487.1"
                     /translation="MELQTDEFAQAAARAAIRFLERYAGSGNEVLANCTERLFQALQN
                     GHSFIRLSGDEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGAGSEGQRDAAALALLQFFMVITGGPGTGKTTTVAKLLA
                     LICGENENLPHIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLSPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSYVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSDAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGKKTFCQAVNTVKVR
                     QTALGSMLERVFSQE"
     gene            complement(2549..3244)
                     /gene="lolD"
                     /locus_tag="ACFB64_001120"
     CDS             complement(2549..3244)
                     /gene="lolD"
                     /locus_tag="ACFB64_001120"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC1209488.1"
                     /translation="MSDLILKCEGVGKRYRDGGLDVPVLYGLDLEIRTGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRALNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSRKEAVETAMAMLDKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPRQGANA"
     gene            complement(3237..4484)
                     /locus_tag="ACFB64_001121"
     CDS             complement(3237..4484)
                     /locus_tag="ACFB64_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC1209489.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFAENRKGILAAAPYVSNQAL
                     LANAGEIRGVQIRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGGKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTYSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLWGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            4710..4988
                     /locus_tag="ACFB64_001122"
     CDS             4710..4988
                     /locus_tag="ACFB64_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209490.1"
                     /translation="MNIRKISALCAVAVFTVSTAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPVSLNGGSGSITVSGSSAAQCVFTVGSGGTLQIQGLNCK"
     gene            complement(5044..5661)
                     /gene="recR"
                     /locus_tag="ACFB64_001123"
     CDS             complement(5044..5661)
                     /gene="recR"
                     /locus_tag="ACFB64_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC1209491.1"
                     /translation="MNSKKQDAFQRLIGALKVLPNVGPKSAQRMAYHLLQQKRKEAEE
                     LVDALQTALRQVRHCARCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNIEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLDGGEIEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(5728..7266)
                     /locus_tag="ACFB64_001124"
     CDS             complement(5728..7266)
                     /locus_tag="ACFB64_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC1209492.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISEHSINNAMQNEQADGGSPWRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQMIV
                     DDPNFHDANGKFSHALLSQYLSQRHMSEDQFVEEIRDQFALQNLVSLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKASEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPAHEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKNLLFEEAKDAVRQAYIRTEAAKLAENKAK
                     EVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLTGLP
                     APVIVEVQAVTPPEDIAAQLPPAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(7315..7689)
                     /locus_tag="ACFB64_001125"
     CDS             complement(7315..7689)
                     /locus_tag="ACFB64_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC1209493.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFGFRDFKKQMPSEAEICS
                     WLEQVPLATLFNKRGTSWRKLDAETQQKALSSTAEAVKLMSEMPSLIKRPVLECGSRV
                     YVGFSEETYDGIFNRQAPCRQG"
     gene            7839..9467
                     /locus_tag="ACFB64_001126"
     CDS             7839..9467
                     /locus_tag="ACFB64_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFC1209494.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFRNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGREGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALDKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            9546..10778
                     /locus_tag="ACFB64_001127"
     CDS             9546..10778
                     /locus_tag="ACFB64_001127"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFC1209495.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPKTHEEYALARTERKTAKGYAGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRAARFAARYGFEIAEETIKLMRQM
                     VENGEADALVAERVWQELAKGLMEKNPRKMIEMLRECGALQVLLPEVDALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPPDILPRHHGHDINGVE
                     PVREVNQRLRAPRQCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHFIAEQI
                     DRARLAQIAPLQKAFRGA"
     gene            11197..11505
                     /locus_tag="ACFB64_001128"
     CDS             11197..11505
                     /locus_tag="ACFB64_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209496.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGETRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            11587..12618
                     /gene="ruvB"
                     /locus_tag="ACFB64_001129"
     CDS             11587..12618
                     /gene="ruvB"
                     /locus_tag="ACFB64_001129"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC1209497.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMGEEGA
                     MEVAKRSRGTPRIANRLLRRVRDFADVKNNGVIDAAVADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(12863..13546)
                     /gene="rpe"
                     /locus_tag="ACFB64_001130"
     CDS             complement(12863..13546)
                     /gene="rpe"
                     /locus_tag="ACFB64_001130"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC1209498.1"
                     /translation="MTAYRIAPSILSADFARLGEEVANVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYVLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRQV
                     RAMLDRYEGKSGRRIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIAAM
                     RAELEKAAG"
     gene            13692..14126
                     /locus_tag="ACFB64_001131"
     CDS             13692..14126
                     /locus_tag="ACFB64_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFC1209499.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYHSSMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(14139..15002)
                     /locus_tag="ACFB64_001132"
     CDS             complement(14139..15002)
                     /locus_tag="ACFB64_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC1209500.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHVFPLSRLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVMVYATSNRRHLMPEYLDENAGTTGVRGEIHQKEAVEEKVSLSDRF
                     GLWLSFYPFDQNDYLAAVRSWLEDFDVPYDEAARTAALQWAQMRGNRSGRSAWQFACD
                     WAGRLPEQRAP"
     gene            complement(15010..15627)
                     /locus_tag="ACFB64_001133"
     CDS             complement(15010..15627)
                     /locus_tag="ACFB64_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFC1209501.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAAENLQHGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFALPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKAGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(15790..16404)
                     /locus_tag="ACFB64_001134"
     CDS             complement(15790..16404)
                     /locus_tag="ACFB64_001134"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFC1209502.1"
                     /translation="MKTSNFPKSPPALKTFALILAGGLADRMGGEDKGLALLEGRPLI
                     DRVIGKIRPQVSHIVISANRNLEEYARRSPHVFPDARQWQHFGPLSALCTAANDLQLA
                     AADWLLIVPCDMPYLPDDLVARFESVSKRTPLCNAFYVETPVTMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            16611..18380
                     /locus_tag="ACFB64_001135"
     CDS             16611..18380
                     /locus_tag="ACFB64_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFC1209503.1"
                     /translation="MILPTRFSDGIPLSLRLKLLTGLWVGLAALSVVLTLLLSFRLEN
                     AASVIEEAGNLKMQAYRLAYMAGEGSPRAQIDNQIAEFEKSLKRISQSDAIHPLIPSD
                     NPLAYDLIQSMLIIDWQANILPPLQAYRRPTQIELYRFAGNIELFLQALENAGEKNTW
                     WLRRFQWVIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGQRHFDIPVPEDGTPE
                     FKQVGRCFNQMALRLKTLYDDLEGQVAEQTHNLEKQNRNLTLLYRTTRDLHQSYTPRQ
                     AAEEFLNHILPAVGAQSGNICLENGSDTDISVHTAEHGKKPPLEKYHDETFPIEYQNE
                     KLGMLSLGFSDGTSLTGDDRTLLQTLIRQLGVSLAGAKQEEEKRLLAVLQERSLIAQG
                     LHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFRT
                     KISNKEFPEAVADLFARFTQQTGITVETVWENGSFLPTQDEQLQMIFILQESLSNIRK
                     HARATHVKFTLSEYGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAVL
                     EIRSQAQQGTTVSLTGAPKESLP"
     gene            18377..19033
                     /locus_tag="ACFB64_001136"
     CDS             18377..19033
                     /locus_tag="ACFB64_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC1209504.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQRTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
     gene            complement(19234..20237)
                     /locus_tag="ACFB64_001137"
                     /pseudo
     CDS             complement(19234..20237)
                     /locus_tag="ACFB64_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_304669545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         4669 a         5848 c         5067 g         4653 t
ORIGIN      
        1 gtgttacgca atatataagg ggttgccgtt ccgcagttgg gcggcagcat acacgttcct
       61 ttcggcgtat tctcgccgtt gcgcggcgaa tatctcgacc tgcttgccca tgcgccgtca
      121 acgggttttc agacggcatt cgatatcggg acgggctcgg gcgtacttgc cgccattttg
      181 gcgaaacagg gcattccttc cgtcatcggc acggatacca atccgagggc ggttgcctgc
      241 gcccgtgcca atattgcccg tttgggtttt gaaaaacagg ttgaaatccg cgaaaccgat
      301 ttatttcccg aagggtttgc cgacctgatt gtctgcaatc cgccctggct tcccgccaag
      361 ccgacttccg ccgtcgaatc cgcgttatac gaccccgaat ctgcgatgct ggctgcgttt
      421 ttgcgtgatg cgccaaacat ctgaatcccg acggagaaat ccgtcttatc atttccgacc
      481 tcgccgtgca tttggggctg cggtctgccg actttttgga aaaggcattt atccgggcgg
      541 gtttgcgtgt tgaggatgtt ttgaaaacca aaccggttca caaaaaagct gccgattcga
      601 acgatccgct ggcttttgcc cgaaacaggg aaactacttt cctgtgccgt ttgaaaaagg
      661 cataaggggc ggtggcgtgc ttccgacggt aggcatcgga acgcccgcgc cgccggcacg
      721 gcatcattcg ggcggattat tcttgtgaaa atacccgctc aagcatactg cccaacgccg
      781 tctgacggac tttgacggtg tttaccgcct gacaaaatgt ctttttgccg ccgaaaaata
      841 cgaatttttc cctggcgcgg gtaatggcgg tatataacag ctccttactc aatccggaca
      901 atgcatcgtc cccttcgtcc gaaggtgcgt cggaaggcgg cagcagccag acttcccgat
      961 attccgaacc ttggcttttg tggacggtca tggcgaatgc gggttcaaat tcgggcaggc
     1021 agcttaccgc taccttttta aatccgtccg catcggcaaa ataggcggca aggctgccct
     1081 gccgtccgac atcttccata atcagtccga tgtcgccgtt gaacagctca agcgcgtagt
     1141 cgttctgtct gatcataatc ggctctccgg cgaaataggc caaatgttcc ggtatgttca
     1201 ttttgcggcg tacatagctg caataggctt cgttgaagtc ttccgcatcc tgccgccaag
     1261 ctgccagaac cacgatatcc gagatgcctg cgtatgcggc ttcgatattg ccgtctttta
     1321 ccgcctgcca ataggctttg tgcgcccggt acaacctttc gacccgagca ttcggactgc
     1381 attccgaatg ttccagttcg tccggaaacc ggtcaaacaa tgcccacgcc ccttcatcgc
     1441 ccgatacggc ggcacgggca aggcagccga tgccgctgtt gtcgccgaag cggtggctga
     1501 acgacagatg ggcggtgttt tgcgccaaca cgggcggatt tgcgctgacg ctgaaaccgt
     1561 gttccggaag gaaaccggcc agcctttggt gcgtttctcc gtccaaaacg gttttttgcg
     1621 acaaaaccga cagcaccgcc ccgattccga cggacgggag ctggttttca tcgcccagca
     1681 gaatcacgcg cgcgccggtt ttgaccgctt tcaaaagttg cagcatcaat gcggtatcca
     1741 acatggaggc ttcatcgaca atcagcacgt cgaacggcag cggacggata tggtcgaacg
     1801 ccgcctgcat tttgggcggg ctcagcttaa gcagtcggtg gacggtttgc ccttccagtt
     1861 tgagcaaatg gcggcggacg gcctccggcg cgtcaaaacc gttgattgca cggtgcagcg
     1921 cgcgcgccat atgtgccgcc gctttgcccg tcggtgcggc aagcgcgata tggggaagat
     1981 tttcgttttc accgcaaatc agtgccagca gtttggcaac cgttgtcgtt ttgcccgttc
     2041 ccggccctcc ggtaatcacc ataaaaaact gcaacagtgc caaggcggcc gcatcgcgct
     2101 gtccttcgct tcccgcgcct tgaaaccatt ttgcgaggtt ttgccgcgcg cctgccgcgt
     2161 cgggggcgga cgtgccggct gccgccaagc gttttatttc ggcagccaaa tcgtattcca
     2221 actgccacat cctgcccaaa aacagccttc tgccttccaa aatcaaaggc gcggcggatg
     2281 ttccgaccac gggtgcgagt gccgacagcg cgtcagcctc gtccccgctc aaacggataa
     2341 acgaatgacc gttttgcaat gcctgaaaca ggcgttcggt acagtttgca agcacttcgt
     2401 tgcccgaacc cgcatagcgt tccaaaaaac ggattgccgc ccttgccgcc gcttgggcaa
     2461 attcatctgt ctgcagttcc atcgtattcc ttgtcgaaat gccgtctgaa ggcacggggc
     2521 ttcagacggt gcgtgttgtt tcggctgttt aggcgtttgc gccctgtctg gggtgcaggc
     2581 tgccgtcttt catgaccatc acgcgctcga agcggccggc gagttcgtcg tcgtgcgtta
     2641 cgaccaccag ccccgttccc aattccgttt tcagttccag catcatatcc aaaacattcc
     2701 ttgcgtttgc ccggtcgagg ttaccggtcg gttcgtcggc aagcaggcat ttgggttgcg
     2761 taaccaaggc gcgcgcaatg gcggcacgct ggcgttcgcc gccggacaat tcgcccgcac
     2821 ggtgcgtcga acggtgtttc agtccgacct tgtcgagcat cgccattgcc gtttctaccg
     2881 cctctttacg gctttttttg ccgatcagaa gcggcatcat cacgttttcc agtgccgaaa
     2941 attcaggcag aagatgatgg aactggtaca cgaaaccgag atggcggttg cgcaaatcgc
     3001 ccaaacgccg ctggtttaag gcacgcaaat cctcgcccat cagcagcacc ctgccttcag
     3061 acggcatatc cagcccgccc aaaatatgca gcagcgtcga tttgccgctg cccgaagagc
     3121 cgatgatgcc ggtgctttcc cccgtgcgga tttccaaatc caagccgtac agcaccggaa
     3181 cgtccaaacc gccgtcacgg tagcgtttgc ccacgccttc gcatttcaaa atcaaatcac
     3241 tcataacgca aagcctccgc cggttgggtt tttgacgcgc gccagctcgg gtacagtgtc
     3301 gccacgaaag acagccccaa ggaaatgcag gcaatcaggg caacgtcgcc catatcgaca
     3361 tcactgggca ggtagtcgat aaaataaacc tgcgaattga tgaggtggac accgagcagg
     3421 ttttcaaaaa acgccacgac cctgccgacg ttccaacccc aaagcacgcc gcagaccaca
     3481 cccgccagcg tgccgaaaaa gcctgaaaac gcgccctgca ccataaaaat cttcatcacg
     3541 ccggcagggg aaagacccaa agtccgcaaa atcgcaatgt ccgcctgctt ttccgtaacc
     3601 gccatcacca aggaagagac aaggttgaac gccgccacgg cgataatcag cgtcaggata
     3661 atgaacatca tccgtttttc cagttcgacc gcttcaaaat agctgcggtt gctgtacgtc
     3721 caatcgcgca cccaaaccgt gtccctttgc gcctccggaa tcagcgttgc cgtcaaggcg
     3781 ggggcgtttt gcggatcggc aagcttcagc cgcagccccg caacttcctt atccaagcgg
     3841 tacagtacgc gcgcgtcttg gatatgcgtc atcgccaatg agttgtccac ttcgtaaaca
     3901 cccgtcttga ccaggccgac cacggtaaac tgcttcaggc gcggcacgac tccggcaggg
     3961 gtaacgttgc cctccggcgt aattaccgta accttgccgc cgacttccgc ccccaaagcc
     4021 tccgccaaac cgacaccgag gataatgtca aactcgcccg gaatcaggtc ttcaaacttg
     4081 cctgccggca ttttgtcgcc gtattccacc actttgcgct cttcagacgg caaaatgccg
     4141 cgtatctgaa cgcccctgat ttcgcccgcg tttgccagca atgcctgatt ggaaacatag
     4201 ggtgcggcag ccaaaatgcc tttgcggttt tcggcaaacc gaagcaggtt gcgccaatcc
     4261 gtatccgtat tgtcgatata gccgatttcg gcgtgcggcg cgacattcag gagctgcccg
     4321 cgtatttctt tctgaaagcc gttcataacc gacaagacga caatcagcgc ggttacgccc
     4381 aaggcgattc cggcaatcga aaccatcgtg ataaacgaca taaagccgtt gcgctttttc
     4441 gccctgagat acctcaagcc tatccaagcc tctagagaaa acataacgct accttaaaaa
     4501 tgtctgcaaa cgtgccgccc cggacggcgg tttgggggag gcggcaaaag tttattgtac
     4561 cgtaaaaccc cggcagcgtc cgaacggcag gctgtgtaaa taaagacgcg gtatgtggca
     4621 aattcggaaa aacaggacgg aaaacttgca caacgcgccg aactacccta tccttctctt
     4681 gaaacaaaac cttttcttta aggaaaacaa tgaatatcag gaaaatctcc gctttgtgtg
     4741 ctgttgccgt ttttactgtt tcgacagcct atgccaaaga aatcaaaatc gatgccaaca
     4801 acacgcctta ttccgaagcc gacgcgcaaa agctggcggc aacggcagtc ggtatgggcg
     4861 ttaaggaacc tgtcagcctg aacggcggca gcggcagcat taccgtttcc ggcagcagcg
     4921 cggcgcagtg cgtctttacg gtcggcagcg ggggcacttt gcagattcaa gggctcaact
     4981 gcaagtaaac cgcccgaaaa aatgccgtct gaaggcttca gacggcattt tgcattggcg
     5041 gcgttacgcc ccgccttctt taatcagacg ccgttcgtac accgcctgcg ccagcgttcc
     5101 cgcatcgaca tattccaatt cgccgcccaa tgggatgccc tgcgacagcc tgctgacctt
     5161 gtaaggcagg tttttgaaaa actcggacag gacatacgct gtggcattgc cttctgcggt
     5221 aaaggcggtc gcgataatga tttcttcgat ttccccgccg tccagccgtt gcgccagcct
     5281 gtccaatgcg atggcggata cgtccattcc caatgccgta ttgatttgcc ccatcaggac
     5341 gaaatacagc ccgtcgtggc agtttgccgc ttctatattc gacacgtcgg caggcatatg
     5401 caccaccatc agccgccgcc cgtcgcgtgt ttcatcggca caaatatcgc acaatccgcc
     5461 ttcgcaaaat gtattgcacc ttgcgcaatg gcgaacctgc ctcaatgccg tctgtaaggc
     5521 atccaccagc tcttcagcct ctttgcgctt ttgctgcaac agatggtacg ccatccgctg
     5581 tgccgatttc ggcccgacgt tgggcaatac tttcagcgca ccgatcaggc gttggaatgc
     5641 atcctgtttt ttggaattca tcatactccg ccatatggga aaacggtcgg aatattccga
     5701 ccgttatgtt gtcaacaaaa gtgtcaatta ctgaccatcg ccgttgtcaa ccgattgtgc
     5761 tcctttagtc tgtttgattt ttccgttgaa atagcggatc agcaggtcga aagtattggc
     5821 agactgctgt tgcgccaaag cctgtttcgc aggaggaagc tgtgcggcaa tatcctccgg
     5881 aggcgtgact gcctgcacct cgacaatcac gggtgccggc agaccggtca gtctgacata
     5941 ggcgggtttg ccgtttgccg gttttgcttt cagcagttcc gcataagcct cgggcggcat
     6001 ggactgcctt gcctgctgcg cgcccaaaac ggacacttcc gaccatttca cgtcaactgc
     6061 cttgccgccg ttcagttggg taagcacttc ttttgccttg ttttcggcaa gtttggcggc
     6121 ttcggtacgg atataggcct gacgcaccgc atctttggct tcttcaaaca gtaggttttt
     6181 ttcttcgcgg acttctttgg cgcggacgac ccacgcggtt tcgctgttga tggtcagcac
     6241 ttcggaattg tgttttttct tcaatacgtc gtcgctgaat acggcattga ttaggttttc
     6301 gggcatgccg gacatttgtg cgtcctgcct gctcagccaa gtttcttggg tttccacttt
     6361 caaaccgctg tttttggcgg cttcggcaag cgaggaggga tgattgaacg catcgtcgcc
     6421 cagcttttct tttgccttgt tgaagtcggc aaccgccttt ttcattttca attcgttttc
     6481 gacggcggct ttttcctgct cgaaagaagg tttggcttca tgtgccggca aacgcgccac
     6541 gcgctcttca aacgcatttt tcacttccgt ttcactgacg gtctgcttgt ctgcaaaatc
     6601 cttcagattc aaggcgacat attccaattt gaccgcctgc ggcagcagat agtctttttt
     6661 gttcgcatta taaaatttct gcaaatcggc ttcagacgct ttgacttggg cgatgaactc
     6721 gtcggggttg aaagtgtgcg aacggatggt gcggttgacc tgcgtcagcc tgatcagctg
     6781 ttccgcctgc gcgtcgccga ccaatacgcc gttttggacg aggcttacca aattctgcaa
     6841 ggcaaactga tcgcggattt cttcgacaaa ctggtcttca gacatatggc gttgcgacag
     6901 gtattgactc aaaagcgcgt gactgaattt gccgtttgcg tcgtggaaat tgggatcgtc
     6961 cacaatcatc tgcttgattt gttcggaaga aaccgaaatg cccatcagct tcgcgccctg
     7021 tttcaggtag gcgcgttgca gcagggattg gaacaccgcg tcgcgccaag ggctgccgcc
     7081 gtccgcctgc tcgttctgca tggcgttgtt gattgagtgc tcgctgattt tttcgtcgcc
     7141 cacttggacg atgtagtcgg cgcccggatg ggaaaccgtg ctgacgccga agccgacaaa
     7201 agttaatgca atcaggccta ataagacttg ggcgggtgtt ctgtattttt cgatggaatg
     7261 gaacatattt tcagattggg aatgggaaat tcaagtcggg tattgtaacg gtttttatcc
     7321 ctgtctgcac ggggcttgcc ggttgaagat gccgtcgtag gtttcttcgc tgaagccgac
     7381 gtaaaccctg ctgccgcact ccaatacggg acgcttgatc aggctcggca tttcggacat
     7441 cagtttgacg gcctccgccg tcgaggacag ggctttttgc tgtgtttcgg catcgagttt
     7501 gcgccagctt gtcccgcgtt tgttgaacag ggttgccaaa ggcacttgtt ccagccacga
     7561 gcagatttcc gcttcagacg gcatctgttt tttgaaatcc cgaaacccaa actccaagcc
     7621 gtatccggca agcctgtttt tggctttttt gactgtgtcg caatttggaa tgccgtgaag
     7681 gattatcatt tggaaacctt ttgcctgaaa taataaaacc gatattttac tataagtgtc
     7741 tgaaaatttg cccgtctgtt tcagacggcg gggcggttat gttacaatcc gaaaattcga
     7801 aaaatttaat ctcttgttca ataaaggttt tacccataat gatttctacc aacggcatta
     7861 ccatgcagtt cggcgcgaag ccgctgtttg aaaacgtatc cgtaaaattc ggcgaaggca
     7921 accgctacgg cttgatcggc gcgaacggtt cgggcaaatc caccttcatg aaaatcctcg
     7981 gcggcgattt ggaacagacc gccggcgaag tggcgattga aaacggcgtg cgtttgggta
     8041 aattgcgcca agaccagttt gcctacgaag acatgcgcgt gctggacgtg gtgatgatgg
     8101 ggcataccga aatgtgggcg gcgatgaccg agcgcgatgc gatttacgcc aatcccgaag
     8161 ccaccgaaga cgactacatg aaagccgccg aactggaagc caagttcgcc gaatacgacg
     8221 gctacaccgc cgaagcgcgc gccgccgaat tattaagcgg cgtgggcatt tccgaagatt
     8281 tgcacaatgc gaaaatggcg gaagtcgccc cgggcttcaa actgcgcgta ttgctggcgc
     8341 aggcgctgtt ctccaagccg gatgtattgc tcttggacga accgaccaac aacttggaca
     8401 tcaataccat ccgctggctg gaaggcgtgt tgaaccaata cgactccacc atgatcatca
     8461 tctcgcacga ccgccacttc ttgaacgaag tttgcaccca catggcggat ttggactaca
     8521 acaccatcac tatttatccg ggcaactacg acgactatat gctcgcttcc gcccaatcgc
     8581 gtgagcgcgc cctgaaagac aatgccaaag ccaaagagaa actgcaagag ctgcaagaat
     8641 tcgttgcccg tttttccgcc aataaatcca aagcccgtca ggcaaccagc cgtctgaaac
     8701 aggccgacaa aatcaaatcg gaaatggtcg aagtcaaacc ttctacccgt caaaacccgt
     8761 atatccgttt tgaagccgat gaaaaagcca agctgcaccg tcaggctgtg gaagttgaaa
     8821 aactggccaa acgctttgaa acccagttgt ttagaaacct caacttcatc cttgaagcag
     8881 gccaacgcct cgccatcatc ggcccgaacg gcgcaggtaa atctaccttg ctgaaactct
     8941 tggccggcgc gtacaacccc gaatattcag acggcctgtt gccggacgaa ggcagcatca
     9001 aatgggcgga aaaagccagt gtcggctact atccgcaaga ccatgaaaac gactttgacg
     9061 tcgatatgga cttgagcgaa tggatgcgcc aatggggtcg ggaaggcgac gacgaacaag
     9121 tcatccgagg cactttgggg cgtttgcttt tcggcagcaa cgacgtggta aaaaaagtga
     9181 aagttctctc cggcggcgaa aaaggccgta tgctttacgg caaactgttg ctgttgaaac
     9241 ccaatgtctt ggtcatggac gaaccgacca accatatgga catggaaagc atcgaatcgc
     9301 tgaacatggc actggacaaa tacaacggca cgctgatttt cgtctcccac gaccgtcagt
     9361 tcgtttcctc cctggcaacc caaatcatcg aactggacgg caaaggcgga tatgaacact
     9421 acttgggcga ttacgaaagc tatctcgaga aaaaaggcgt agcataagcg cacccctccg
     9481 gttgaaacaa tgccgtctga agccgcttca gacggcattg ttgataactt taaaatagga
     9541 agcatatgca gacttacctc gtcggcggcg ccgtccgcga ttatcttttg ggcctgcccg
     9601 tcaaagaccg cgattgggtg gtcgtcggcg cagacgcgca aaccatgttg gcgcaaggtt
     9661 tccagccggt cggcaaagat tttcctgtat tcctccatcc caaaacgcac gaagaatacg
     9721 ccctagcccg caccgaacgc aaaaccgcca agggttacgc cggtttcagt ttccacgccg
     9781 acaaagacgt tacgctggaa caggacttga tgcgccgcga cctgaccatc aacgcgatgg
     9841 cgcaagatgc ggacggcaag attatcgacc ctttcggcgg gcaacgggat ttggcggcag
     9901 gcattttgcg ccacgtctca cccgccttcg ccgaagatcc cgtccgcatc cttcgtgccg
     9961 cccgctttgc cgcgcgttac ggctttgaaa tcgccgaaga aaccataaag ctgatgcggc
    10021 agatggtgga aaacggcgaa gcggacgcat tggttgccga acgcgtctgg caggagttgg
    10081 cgaaaggttt gatggaaaaa aatccgcgca aaatgattga aatgttgcgc gaatgcggcg
    10141 cactccaagt cttgctgccc gaagtcgacg ccctcttcgg cgtgccgcaa cgcgccgact
    10201 atcatcccga aatcgacagc ggcatccata ccctgatgac gctgcaacgc gccgccgata
    10261 tgggtttgag cctgcccgaa cgctatgccg ccctgctgca cgacttgggc aaagccaaaa
    10321 caccgcccga catcctgccg cgccaccacg gacacgacat caacggcgta gaacccgtgc
    10381 gcgaagtcaa tcaacggctg cgcgcgccga ggcagtgtgc cgagcttgcc gaattggttt
    10441 gccgttggca cattattttc caccaagtcg gacagcttaa aagccaaacc attctgaacg
    10501 tattgaaaaa aaccgatgcc ttccgccgtc ccgagcgttt tcagacggca ttgaacgtct
    10561 gcattgccga cacgcaaggc cgtctgaacc gagaacacac gccctacccc cagcgcgcgc
    10621 actggctcgc cctgctcgaa gccgccaatc aggcggattc gggcaaaatc gccgccgaat
    10681 gccgcgcgca gggaaaagcg cactttatcg ccgaacaaat cgaccgggcg cggctggcgc
    10741 aaatcgcccc gctgcaaaaa gcgtttcggg gcgcgtagga aaaagaaaac gccaaacgcg
    10801 gcatcttttg aggttcgccc gcattgccga aacagcgtcg gagaaaggga tggatgacat
    10861 cggtttattc ggctgccttt taatattccc ttgatgcagg cctgattagt cggacaaaaa
    10921 taccttttgt tcaacggaac cgatttctat ccggacggaa acagccggct ttccccttgc
    10981 aaacggatgg aatcaagcgg gtattcaaac acagccgttt atttttattc cgccgccaag
    11041 ttatgggata gggtttgcgt gtttcgggca ggcaaaccgc gcaaccttag acgcagtccg
    11101 aagttttgaa ggcggcatcc gggccgggtt ttaaaacagc cgccgccata tccgctataa
    11161 tccatccttc agccattccg cccccgacat aaaatcatga ccctgaaaac cgatttattg
    11221 cctaaaatca acaacgaaga ttatcaacgc ctcatcctca aacatagcgc ggaattcagc
    11281 ggcggagaaa cccgcctgtt gaacgaaatc ctcgaaaaat tcaatttcga cgttgttcag
    11341 gcgcaggcat tggcgcaggc ggtaatgcag caagttcgct tcgatcccaa cgcctaccac
    11401 atcgacagcg acgacgaaga caccaccggc atctgccccc actgcatcaa cccgcctatg
    11461 ccgcccctgc gcgactatct cgtttggcgc gaaacacgcg gataaaacgc ttttgaccgt
    11521 tatcttttca atgccgtctg aaacgccgcc gaccgttcgg acggcatacc cgacaaaggg
    11581 aacactatgc tgcaaaccga caacctgacc gccgcgcaac cgcaacgcat cgttgccgcc
    11641 caaaccgcct ccgcacagga agaactgctc gaacgcgccc tccgccccaa aacgctggac
    11701 gactacatcg ggcagcataa agccaaagag cagctcgcca tcttcatcca agccgccaaa
    11761 aaacgcggcg aagcactcga ccacgtcctg cttttcggcc cgcccggact gggcaaaacc
    11821 acccttgccc acatcatcgc caaagagttg ggcgtaaatt tgcgccaaac cagcggcccc
    11881 gtcctcgaac gcgccggcga cctcgccgcc cttttgacca accttgatcc gcacgacgta
    11941 ttgtttatcg acgaaatcca ccggctaagc cccgtcgtcg aggaaatcct ctatcccgcg
    12001 ctcgaagact accggctcga cattatgata ggcgaaggac ccgccgcacg ttccgtcaaa
    12061 atcgacctgc cgcccttcac cctcgtcggc gcgaccaccc gcgccggtat gctgaccaac
    12121 ccgttgcgcg accgcttcgg catcgtctcc cgcctcgagt tttacgaaaa ccgcgacctt
    12181 accaccatcg tcagccgttc ggcacaactg ttgcagctcg atatgggcga agaaggcgcg
    12241 atggaagtcg ccaaacgcag ccgcggcacg ccccgcatcg ccaaccgcct gttgcgccgc
    12301 gtgcgcgatt tcgccgacgt gaaaaacaac ggcgtaatcg atgccgccgt cgccgatgcc
    12361 gctttaagta tgctggacgt ggacgcgcag gggctggacg tgatggacag gaaattcctc
    12421 gaagccgttt tgcacaaatt cggcggcggc cccgtcggtt tggacaacgt tgccgccgcc
    12481 atcggcgaat ctacggacac catcgaagac gttatcgaac cctacctcat ccaacaaggc
    12541 tttttgcaac gcaccccgcg cggcaggatg gcgaccgaac gcgcctacct gcatttcggg
    12601 ctgcccgtcg aaaaataaac catgccgtct gaaaccgaac cgggtttcat acggcattgt
    12661 tatcagacgt ttgcaccggc accggtttca tcacttaaca gaaacctttt gtccggcagg
    12721 acagattctc cccgatgaaa ataactgttt ccctggcaaa cggttgaaac ccgtctggct
    12781 gccggttgaa aaataacgca atgctgtctg aaacagagct aattttcaga cggcatttct
    12841 atttcaatca ttggctcaag gttcagcctg ccgctttttc cagttccgcc ctcatcgcag
    12901 cgattaccgc cttgtaatcc ggtttgccga aaatcgccga accggcaaca aaggtatccg
    12961 caccggctcg ggcaacggcg gcaatattgt cagttttgat gccgccgtcc acttcaatgg
    13021 caatacgtct gccgcttttc ccttcatagc ggtcaagcat ggcgcgaacc tgtctgattt
    13081 tttccaaagt gtgcggaatg aagctctgac cgccgaaccc ggggttgacc gacatcagca
    13141 aaaccatatc cagcctgtcc aacacgtttt ccaacacata tacgggcgtt gccggattca
    13201 acaccagccc cgcctgacag cccatgtccc gaatcaggct caagctgcgg tcgatatggc
    13261 ggctcgcttc gggatggaaa gtgataatcg atgctcctgc tttggcaaac gattgaatta
    13321 aatcgtcaac gggttcgacc atcagatgca catcgatcgg cacgcttgca taaggcttca
    13381 acgccgcgca gaccataggt ccgaaggtca ggttcggcac ataatggttg tccatcacgt
    13441 caaaatggat cagatccgca cccgccgcaa tgacatttgc cacctcttct ccgaggcggg
    13501 caaagtctgc cgacaaaata ctgggtgcga tacggtaggc ggtcatattt tttccttcaa
    13561 tgcctttatg ggcggactca acgcgggaaa gcctcgaaac agcattatta acaaaagtta
    13621 actgccataa taccatcttt cacacggcaa tcaagtatat tgtccgaaat atccggatta
    13681 aaggaaaagc cgtgccgctg cctgctccct gccgttttgc caaacctgcc gcctcttttt
    13741 taagtatggc tttgctttcc tgccagcttt cccacgccgc cacggcttat atccccccga
    13801 acgattttca accgaactgc gacatacgcc ggctcgggct gacacagggt cagcacaatg
    13861 agctgcgtaa aatccgcgcc gccttcaaaa tggcgggcga cagggcgcgt ttgaaggtta
    13921 tgcattccga acacagccgc cgccgctctg tcgtcgaaat catttcttcg gatgttttta
    13981 atcggaacga ggcgcgcgat tatgtcgaaa gccgctacca ctccagcatg gattttgcgg
    14041 tggacgaatt ggaaatccaa caccgcttct tccatattct cacaccgcaa cagcagcaaa
    14101 tgtggctttc ttcctgcctc aaataatccc cgaaacactc acggcgcacg ttgctccggc
    14161 agcctgcccg cccagtcgca ggcaaactgc caagcggaac gtcccgagcg gttgccccgc
    14221 atctgcgccc actgcaacgc cgccgtccgg gcggcttcgt catatggcac atcaaaatct
    14281 tccaaccagc ttcgcactgc cgccagataa tcgttttgat cgaacggata aaaactgagc
    14341 cacaatccga atcggtcgga caaagatact ttttcttcca cggcctcttt ttgatggatt
    14401 tctccgcgca cgcctgtcgt acctgcattt tcatccaaat attcgggcat cagatgccgt
    14461 ctgttggaag tcgcgtacac catcacgttg gcgcaacgtt gggacagacc gccatccaat
    14521 gccgttttca atgccttata ggtttcgtcg ccgctttcaa atgacaaatc gtcgcaaaac
    14581 acaatgaatt tttccgggcg ttccttcaaa agtgtcaaca gatacggcag gccgatcaaa
    14641 tcgcttttat cgacttcgat caggcgcaat cccttatccg catattcgtg cagcagggct
    14701 ttgaccagcg aggatttgcc tgttccgcgc gcgccgctca tcaatacgtt attcgcaggt
    14761 ctgccggcaa taaattgttc ggtattacgc accagcaatt cggtctgcct gccgactccg
    14821 gcaagccgcg acaaagggaa aacgtgcgga tcgggcaagt gttccaaaaa accttttttg
    14881 cccgcactct gccagcggaa ggcaagcgcg ttccaatccg tatgcccggg ttcgggcgga
    14941 agcacggcat ccaaacgccg caaaacggca taggctttat cgagaaattc gttcaattcc
    15001 atctctacct cactttgcat atctttgcgc catcagccgt tcgacggtgt cgacgattgc
    15061 ctgcgtattc ggatcgattt cgatgttgat cctgtctccg gcctttctgc tgccgaacag
    15121 cgtccgttcc aaagtttcgg gaatcagatg gacattgaaa cggctgtctt cgactttgcc
    15181 tatggtcagg ctgcaaccgt ccaagccgac gaaccctttg gtcaggatat agggtttgag
    15241 ttcgtgcggg agcgcaaacc aaaccgtgcg gttgaacccg tcccgttcga tttcgacaat
    15301 gggcacggtt gccataatgt gtccgctcat gacgtgtccg ccgatttcgt cgccgaaacg
    15361 tgccgcccgt tcgatgttga cgcaatcgcc ttccttcagc agccccaaat tggtttttgc
    15421 caaagtttct gccattaaat cgaagctgac gcggtttcct tcgatttcag taatcgtcag
    15481 gcagcagccg ttattggcga ccgatgcgcc gtgttgcagg ttttctgccg cctcttgcgg
    15541 aagctcgacg acataagtct gaaacgcctc cgacgggcgg tggattgccg tcagttttcc
    15601 caatccttga acaatgcctg taaacataat cctgtttccc tgtgtcggta aaaatggtgc
    15661 aaattgtagc atttccccgc gaaaaatgcc gtctgaaact ccttcagacg gcattgtgcc
    15721 tccggttcgg gcaaaaaccg cccggtacgc cttgaccttt cctttccgcg ccggtcggcg
    15781 gtcttgcact tatccctcct gcaaatcggt ttgcgtgttc aagtcggcaa aatgcccgtc
    15841 aaactcgaat ctgaccggcc gcgccctttg ctgctgcaac cagcttctta atgttttcat
    15901 acccgaaaac agataaggaa tcgcgctttg cagaatttgc ggacggatat acataatgtt
    15961 gtagtgcatc gttaccggcg tttccacata aaacgcattg cacaacggtg tgcgtttgga
    16021 gacgctttca aagcgcgcga ccaaatcgtc cggcagatac ggcatatcgc aaggtacaat
    16081 cagcagccaa tcggccgccg ccaactgcaa atcgttggct gcggtacaca atgccgaaag
    16141 cgggccgaaa tgctgccact gccgggcatc cggaaaaaca tgcgggctgc ggcgggcgta
    16201 ttcttccaaa ttccggttgg cactgatgac gatatggctg acctgcggcc tgattttacc
    16261 gataacgcgg tcaatcagcg gtctgccttc caacagtgcc agccctttgt cttccccgcc
    16321 catacggtcg gccaggcctc ctgccaaaat caatgcgaac gtttttaatg cgggcgggct
    16381 tttgggaaag tttgaggttt tcatagtttc agtctgccgt ttccgatatt ttagaagcgc
    16441 gtttgaaaac ggcgtcgtat aaattcatgt tatttgaatt ttatcataac gtgatttaac
    16501 actgaaagca ttgaggctta agttttcctt aacctgtcct ttctgagcag ttgtttctaa
    16561 ttccaaagaa tgatattgtt tgcaccgttt aaaacgactt tcctccgaat atgatactgc
    16621 caacccgatt ttcagacggc atcccccttt ccctgcgcct gaaactcctg accggcttgt
    16681 gggtcggttt ggcggcactg tctgtcgttt taacgctgct gctttctttc cgcctggaaa
    16741 atgccgcctc ggtcatcgaa gaggcgggca atctgaagat gcaggcatac cggcttgcct
    16801 atatggcggg cgaaggctcg ccccgcgcac aaatcgacaa tcagattgcc gaatttgaaa
    16861 aaagtttaaa acgcatttcc caaagcgatg ccatccatcc gctgattccc tccgacaatc
    16921 cccttgccta cgatttgata cagtccatgc tgattatcga ctggcaggcc aacatcctcc
    16981 ccccgttgca ggcataccgc cgccccacac aaatcgagct ttaccgcttt gccggcaata
    17041 tcgaactgtt tttgcaggcg ttggaaaatg ccggcgaaaa aaacacttgg tggctcaggc
    17101 ggtttcaatg ggtcattatg ctgatgacgc tggtgtcgtc cgtgctgatg cttttttggc
    17161 accagatttg ggttatccgt ccgctgcagg cgttaaggga aggtgcggaa cgcatcggtc
    17221 agcggcattt cgacatccct gttcccgaag acggtacgcc cgaattcaaa caggtcgggc
    17281 ggtgtttcaa ccaaatggcg ctcaggctga agactttata cgatgatttg gaaggacagg
    17341 ttgccgaaca aacccacaac cttgaaaagc aaaaccgcaa cctgaccctg ctgtaccgga
    17401 ccacgcgcga cctgcatcaa tcctatacgc cgcgacaggc ggcggaagaa tttttgaacc
    17461 acatcctgcc cgccgtcggc gcgcaatccg gcaacatctg cctggaaaac ggttccgaca
    17521 cagacatttc cgtccatacg gccgaacacg gcaaaaagcc ccctttggaa aaataccatg
    17581 acgaaacctt ccccatcgaa tatcaaaacg agaaactggg catgctttcg ctcggctttt
    17641 cagacggcac ttccctcacc ggagacgacc gcacgctgct tcaaaccctg attcggcaat
    17701 tgggcgtatc gctcgccggg gcgaaacagg aggaagaaaa acgtctgctt gccgtattgc
    17761 aggaacgcag cctgattgca caggggctgc acgacagcat cgcgcaggca ttaaccttcc
    17821 tgaacctgca ggtacagatg ctggaaaccg cctttgccga aaacaaacgg gaggaagccg
    17881 cagaaaacat cggctttatc aaaacaggcg tgcaggaatg ttatgaagat gtccgcgaac
    17941 tgctgctcaa tttccgtacc aaaatcagca ataaagaatt tcccgaagct gttgccgacc
    18001 tgttcgcgcg cttcacgcaa caaaccggga taacggtcga aaccgtttgg gaaaacggtt
    18061 cgttcctgcc tacacaggac gagcagctcc aaatgatttt catcctgcaa gaaagcctgt
    18121 ccaacatccg caaacacgcc cgcgccaccc atgtaaaatt caccctttcc gaatacggcg
    18181 gacgctttac catgaccatc caagacaacg gacaaggttt cgacacggag aaaataggag
    18241 aacctacggg cagccatgtc ggactgcaca tcatgcagga gcgtgccaaa cgcatccgtg
    18301 ccgttttaga aatccgttcc caagcacaac agggaaccac cgtctcattg acgggcgcac
    18361 ccaaagaaag cctaccatga ccatcaaaat catcctgata gacgaccaca ccctcttccg
    18421 cagcggcatc aaagcccttt tgtcgcgcca acacggtttt gaagtcatcg gcgaagccgc
    18481 agacggactc tcgggcatca aaatgatcag tcggctgcaa cccgatgtcg tcctgctcga
    18541 tttggatatg ccaggcatga acgggcgcga ggccttgtcc caaatcatca gcatcaatcc
    18601 gcagcaggca gtcattatgc tgaccgtttc cgaagacagc gacgacctta ccgaatgtat
    18661 gcgcatcggc gcgcgcggct acctgctgaa aaacatcaac gccgattttc ttctcgaaag
    18721 catacgcaaa gccgccgaag gcgataatgt attctcgccc gagatgaccg ccaaactcgt
    18781 caaaagcctg atttcccccc aacctgccca aaggacgcag gcactctcct cacttacccc
    18841 tcgtgaactg gaaatcttgg gctatctcgc cgcaggacac agcaacaaaa tcatcgcccg
    18901 ccacctcgat cttgccgaat ccaccgtcaa agtccacgtt caaaacctgc tccgcaaact
    18961 caacctcagc agccgggtgc aggccgccgt ttacgccatc cggcacaacg tcccccaacc
    19021 tgtgccggaa tagacgttca gacggcatcg ggtcaggaaa aaagcaatgc gcccctttga
    19081 cggggtgcaa tatataaggg acggcacacc ggatgccgtc cctttaagta cgccattacc
    19141 gccgtaaccc ggacggaatg gcttaggcag tttatttcca agaatatttc acacccacgg
    19201 tgccttggta gcctttatgt ctgttgcttc cgcatcctgc cgataattgc cttgcctgtc
    19261 ccgagaaagg gtttgatttt cgccacggcg ttggtggagc gtatccagca tcgcatagcc
    19321 tgattccaag ttttgacgtg gaacgagtgc gtaggccggt gcgcttggcg tgatgatggt
    19381 tttatcttga ttcggcgtgg ttaaagtcca gaagtattct gtcgcgcccg cctcatcttt
    19441 gtgtgaaacc aattgcaatt cccccgcccc tgtggttttg gcagttccgc taaaagtatc
    19501 gcgcattgtg taggtatgtt tagcgtcttc gatatttaat ttaccggttt ccgccccctt
    19561 gtcttgtccc aaaaccttaa caacggcggc actgcgtttg tagcggtcgg aaagttcgcc
    19621 gatcgagccg tcgataatat tttctttgcc atctttaccc actgaaatga ccgttgtttt
    19681 tcctgaagca tcgcctgtga tttccagtaa atcagattga gaatttcctc cttgatcatc
    19741 acccggcgag ttccattctg tattgacttt taacacaccg tctttaccga cataatttcc
    19801 ttcaatggtg agtttgtctg cataggattg attggcaagc gtaatgatgc cgccgttggt
    19861 gaatgttcct ttttcaaata cagaagttgt acccgataaa gaaatacctg tattagatag
    19921 ggttagcgtg ccgcctgata ataatgtagt cccttcgggg gttgttaatg atgtgtcttt
    19981 ttttaggtga aaagtagctt ttgttagttt tatggttcca atatcttctg atttgaattc
    20041 cattgcgcca tttgtaattt ttagcacagc attggtaata gggaggggga agcgcattgt
    20101 aaatgatttt tctgttccat ctggattgat taatgtacca gatgcgccaa agatctctcc
    20161 ttcagggata gaaacagttc catcagaaat tgatggttta tttgcgcttt tatcactgtg
    20221 tttatgaatt aatgtaa
//
LOCUS       DAXDXW010000037        19714 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXDXW010000037 DAXDXW010000000
VERSION     DAXDXW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19714
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.038"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(191..1198)
                     /locus_tag="ACFB64_001138"
     CDS             complement(191..1198)
                     /locus_tag="ACFB64_001138"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC1209505.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNTVYKLGLIPVTDNRTKPI
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFLEIGTVTRNPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDAMIRNIEKSKYQGVLGINIGKNAVTPIQNAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGGELGALLEALKNKQAQLAAAHGKYIPLAVKIAPD
                     LDEAQIEDIARVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGFPVREKSNRVL
                     KKLAEHIDGALPIIGVGGIMEGGDAAEKIRLGTTAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            1430..1666
                     /gene="acpP"
                     /locus_tag="ACFB64_001139"
     CDS             1430..1666
                     /gene="acpP"
                     /locus_tag="ACFB64_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC1209506.1"
                     /translation="MSNIEQQVKKIIAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            1822..3069
                     /gene="fabF"
                     /locus_tag="ACFB64_001140"
     CDS             1822..3069
                     /gene="fabF"
                     /locus_tag="ACFB64_001140"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC1209507.1"
                     /translation="MSQRRVVITGLGQVSPVGNTAAEAWDNLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEATGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHACKTVISST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            3462..4687
                     /locus_tag="ACFB64_001141"
                     /pseudo
     CDS             3462..4687
                     /locus_tag="ACFB64_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4692..4814
                     /locus_tag="ACFB64_001142"
     CDS             4692..4814
                     /locus_tag="ACFB64_001142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209508.1"
                     /translation="MPSETFRRHFPNTPPVAGLHGTQISLIKPQITAAIKPPFK"
     gene            5041..6168
                     /locus_tag="ACFB64_001143"
     CDS             5041..6168
                     /locus_tag="ACFB64_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC1209509.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGVNPFSDIKSTFLIFKALKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRITLPMLESLIVLNPDD
                     KDELLHQYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKGKYPDTVFTALGAIDKPRGSGDLEQPAARDIIRFPGFVNNVSEVIKEHH
                     IFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEK
                     MIYFIENRAAVRLMGNASYAIAKDKFDAEKVDLKFLDILKA"
     gene            complement(6570..7420)
                     /locus_tag="ACFB64_001144"
                     /pseudo
     CDS             complement(6570..7420)
                     /locus_tag="ACFB64_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
     gene            7770..9284
                     /gene="katA"
                     /locus_tag="ACFB64_001145"
     CDS             7770..9284
                     /gene="katA"
                     /locus_tag="ACFB64_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC1209510.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLTQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPASYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRSQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFDNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            9800..10366
                     /locus_tag="ACFB64_001146"
                     /pseudo
     CDS             9800..10366
                     /locus_tag="ACFB64_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
     gene            10588..11751
                     /locus_tag="ACFB64_001147"
     CDS             10588..11751
                     /locus_tag="ACFB64_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HFC1209511.1"
                     /translation="MSFKLRYLASVLALSSLLAACGGQEKSAAGDASPASETEAASQV
                     QASEAVPSASSASPEDQDLLKRAQGVFQPLPTVEEMQKIRPFTEEQVKLGHQLWYEPR
                     LSKGNTVSCNSCHNLASAGVDNMPTSQGHKGQFGGRNSPTALNAALLGSQFWDGRAAD
                     VEEQAGGPLVNPVEMANDSQEAAAAKIAKVPEYQEMFKKAFPEDGAVSFKNITTALGA
                     FERTLLTPTKWDEYLKGNVNALSEQERKGVRAFMDNGCIACHNGVNLGGTTFQKFGLV
                     QGPYWKFIEDPKRDKGRADVTKKTEDEFFFRVPGLRNVAKTYPYFHNGSVWELDKAVT
                     IMGKAQLGKDIPKEDVDNIVVFLNALSGNVSESARTMPELPLTAPMESKPDNK"
     gene            complement(12332..14368)
                     /locus_tag="ACFB64_001148"
     CDS             complement(12332..14368)
                     /locus_tag="ACFB64_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC1209512.1"
                     /translation="MIDNALLHLGEEPRFNQIKTEDIKPAVQTAIAEARGQIAAVKAQ
                     THTGWANTVERLTGITERVGRIWGVVSHLNSVVDTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFATLSPAQKTKLDHDLRDFVLSGAELPPERQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYAGNRELREQIYRAYVTRASELSNDGKFDNTANIDRTLENALKTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFAREHLGLADPQPWDLS
                     YAGEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFAEKTVPVWHKDGRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFADGTLQLPTAYLVCNFAPPVG
                     GKEARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSESDECR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFANSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(14456..15328)
                     /locus_tag="ACFB64_001149"
     CDS             complement(14456..15328)
                     /locus_tag="ACFB64_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC1209513.1"
                     /translation="MEIWNMLNTWPDAVPIRAEAAESVAAVAALLLARALLLNIHFRR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RYLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPAGSETL"
     gene            15594..17984
                     /gene="gyrB"
                     /locus_tag="ACFB64_001150"
     CDS             15594..17984
                     /gene="gyrB"
                     /locus_tag="ACFB64_001150"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC1209514.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKI
                     VGDSDKKGTTVRFLAGTETFGNIEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVINQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVATLITALGAGIGKEEFNPEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEAALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGLVGEGAKLYKGENEYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            18083..19468
                     /locus_tag="ACFB64_001151"
     CDS             18083..19468
                     /locus_tag="ACFB64_001151"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFC1209515.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQTARIAIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLGRIRTQHILRLNGQEIRFIP
                     DRDLNIRGNQVLPKHPNGLRFTAYASDGTVLKEQVYYSVGGGFVVTEEDFEWQSETEK
                     AVPYPYNSCAGLLARCRLNRLDISEVVLANEAALAGCGEAEIRRRVAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(19539..19637)
                     /locus_tag="ACFB64_001152"
     CDS             complement(19539..19637)
                     /locus_tag="ACFB64_001152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HFC1209516.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
BASE COUNT         4596 a         5479 c         5290 g         4349 t
ORIGIN      
        1 ctccggttgc ctgccccgtg ttcacgcatc ggtttgcctg ccccgtcatt ccgccccttt
       61 cgattgacgc ctgttacgaa aagtcttcca aatgacggct gattgcctct tcaacagtcg
      121 gataccaatc tatgaaaatg ccgtctgaac ggcaaaacgt gcgttcagac ggcattttgg
      181 gtggggcgcg tcatcgcgcc aatgccttca aacattcttt gaccaacgcc gggcctttgt
      241 atatcaatcc gctgtacact tggacggcgg tcgtgcccaa gcggattttc tctgccgcgt
      301 ccccgccttc cataatgccg cctacgccga taatcggcaa tgcgccgtct atgtgttccg
      361 ccagtttttt cagcacccgg ttgctttttt cgcgcacggg aaaaccgctc aaaccgccct
      421 gctcgcctgc gagcggatgg ctgccgaggc ttgatttgtc gatggtggtg ttggtagcga
      481 tgatgccgtc catttcgacg gatttgacga cgcgggcgat gtcttcgatt tgtgcttcat
      541 ccaaatcggg ggcgattttg acggctagcg ggatgtattt gccgtgggcg gcggcaagct
      601 gcgcctgttt gtttttcagg gcttcgagca atgcgcccaa ttcgccgccg ccttgcagcg
      661 cgcggaggtt tttggtgttg ggcgaggaaa tgttgacggt aatgtagctt gcgtgcgcgt
      721 aggctttttc gaggcagatt aaataatcat cggcggcgtt ttggatgggc gtaaccgcgt
      781 ttttgccgat gttgatgccc aatacgcctt gatatttgct tttttcaata ttgcgtatca
      841 tcgcgtcgat gccgtggttg ttaaaaccca ttcggttgat gatgccttgg tgttcgggaa
      901 cgcggaagag gcgcggctgc gggttgccgg gctgcgggtt gcgcgtaacc gtgccgattt
      961 cgaggaagcc gaaaccgagc gcgcccaaag cgtcgatgta ttcaccgttt ttgtcgagac
     1021 ctgcggcaag cccgacaggg ttgggcaaat ccatacccat cagttttata ggtttggtac
     1081 ggttgtcggt tacgggaatc aggcccaatt tataaaccgt gttgagcgcg tcgagcgtga
     1141 agtggtgggc tttttcggca tcgagtgcaa acaggatgcg gcgggcaagt ggatacatga
     1201 cagggctttc tgcttcagat ggaaatgccg ccattttaac cgaaatcccg ccaaacatac
     1261 ggcacacagg tcaaacttga acgacaaaac ccgccgcacc caaaaatgta caaatccgca
     1321 aatattgata taataggcat tagatagaaa actctattaa aaatcggtac aataccaccg
     1381 atttatttcc cttaaacaac ctgccgcaag gcataaagga acgactgata tgtcaaacat
     1441 cgaacaacaa gttaagaaaa ttattgctga acaactgggc gtaaacgaag ccgacgtgaa
     1501 aaacgaatct tccttccaag acgacttggg cgcggattct ttggataccg tggagctggt
     1561 tatggctttg gaagaagcct tcggctgcga aatccccgac gaagatgccg aaaaaatcac
     1621 caccgtccaa ctggctatcg actacatcaa tgcccacaac ggctaaccgg tcgtcgcccg
     1681 acacaacagc ctctgctgca ccgcgcaata gaggcttttc ccttatatgg caaactgttt
     1741 gaacccctgc cgcaaattac agacggcatc tgcacgacag ccgcatatgc ccccattccg
     1801 acaaccaaca gcgaggttat catgagtcag agaagagtag tcattacagg cttaggtcag
     1861 gtttcccctg tcggcaacac tgccgcagag gcttgggaca acctgctcgc cggcaaaagc
     1921 ggcatcggcg cgattacccg ctttgacgca tccgacatca acagccgtgt cgccggcgaa
     1981 gtgcgcggtt tcgacatcgg acaatacatc agcgcgaaag aagcgcgccg gatggacgtg
     2041 ttcatccact acggcatcgc cgccgcattg caggcgattg ccgattcggg tttggacgat
     2101 gtggaaaacc tcgacaaaga ccgcatcggc gtgaacatcg gttccggcat cggcggactg
     2161 cccagcatcg aggccaccgg caaagccgta atcgaaggcg gcgcgcgcaa aatcaaccct
     2221 ttctttatcc ccggttcgct gatcaacctg atttccggac acgttaccat cctcaaaggc
     2281 taccgcggcc cgagctacgg catggtttcc gcctgtacca ccggcgcgca cgccatcggc
     2341 gattccgccc gactgatcaa atacggcgac gcggacataa tggttgccgg cggcgcggaa
     2401 ggcgcaatca gcaccttggg cgtgggcggt tttgctgcga tgaaagccct ctccacccgc
     2461 aacgacgacc ccgccaccgc ttcccgtccg tgggacaaag gccgcgacgg cttcgtcatc
     2521 ggcgaaggcg cgggcatatt ggtgttggaa gaattagaac acgccaaaaa acgcggcgcg
     2581 aaaatctacg ccgaaatcgt cggcttcggc atgagttccg atgcttacca tatcaccgcg
     2641 ccgaacgaag aaggccccgc ccttgccgtt acccgcgcgc tgaaagatgc cggcatcaat
     2701 cccgaagacg tggattacgt caacgcgcac ggcacgtcca cccccttggg cgatgccaac
     2761 gaaaccaaag ccctcaaacg cgcgttcggc gaacacgcct gcaaaaccgt catcagctcg
     2821 accaaatcca tgaccggcca cctgctcggc gcggcgggcg gcgtggaggc cgtgtacagc
     2881 attttggcga tacacgacgg caaaatcccg ccgaccatca acatttttga acaagacgtt
     2941 gaagccggct gcgatttgga ctactgcgcc aacgaagcgc gcgacgcgga aatcgacgtt
     3001 gccatttcca actccttcgg cttcggcggc accaacggta cgctggtctt caaacgcttc
     3061 aaaggctgat tccgcaaagc cgccgccgac atcgaaatgc cgtctgaaac cgtttcagac
     3121 ggcattttta tagtggatta acaaaaatca ggacaaggcg gcgggccgca gacggtacaa
     3181 atggtacgga accggttcgc ccggtgcttc agcaccttag ggaatcgttc tctttgagct
     3241 aaggcggggc aacgccgtac cggtttaaag ttaatccact atactaactg gaattaacaa
     3301 atacggcaca aatattaacc cgaatcgcaa acgcccatca agccctttca gacggcatcg
     3361 gcagacatac cgttggctat atccaaagaa tcaggaatta tttagcccga atcatccggt
     3421 ttgttaaaat tcgcaaactt aaccgactca aaatacatat tatgacgcac accgcatcga
     3481 aaacacccaa actctgggcg gtcattgccg ccgccgcatt catcctgctg attaccatcg
     3541 gcatgaggat gacgctcgga ctgttcgtcc aacccgtcgt caacaccacc gaattgaaca
     3601 ttgcccagtt cagcctcatc atcaccgttt tccaactgat gtggggcgta ttgcaaccat
     3661 tgtccggcgc gcttgccgac cgtttcggcg cgttcagggt attaagcggc ggtgccctcc
     3721 tgctcgtctg cgcctgcctg attgcctcca acatcccgac ttattggggg ctgatgattg
     3781 ccgtcgggct gctgctcgca ttcggcaccg gttccggcgg gttttccatc attatgggac
     3841 aggtcgccgc ccaagtcccc acacacaaac gcggcttggc atccggactg gtcaacgcag
     3901 gcggttcggc aggacaattc ctgttcgcac cgctggttca aggactcaag gactcgtcgt
     3961 cctgcccgaa gtcggctgga cgggtacatt ttacgtttgg ggcgcaatcg ccctgctgat
     4021 tctgcccgtc tcatggtggc ttgccggcgg caacaacgcc gcccacaccc aacacaccca
     4081 agccacacac ggacaaagcc tcggagaagc agtcaaaacc gccttcaaaa ccccaagcta
     4141 catcctgctg cacctgagct ttttcgcctg cggcttccac atcgcctttc tcgtaaccca
     4201 cctacccacg gaagtcgccc tgtgcggact gcccgccacc gtcgcctcga catccatcgc
     4261 catcatcgga ctggcaaaca tcgccggttg cgtgttctca ggctgatgca ccggacgctt
     4321 caaaggaaaa cacatcctgt tcggactcta cgcctcacgc gtcgccatgg tgctgattta
     4381 catcttctca cccaaaaccg acctcaactt ttacattttc gccgccgcac tcggattcac
     4441 atggctcgcc accgtcgccc cgaccgccgc cgttaccggc aaactcttcg gcacgcgcta
     4501 cctcgccacc ctgttcggac tggtgatgct cacccaccaa atcggcggat tcctcggctc
     4561 gtacatcggc ggcatcgtga ttacacaatt cggtgactac ggctggatgt ggtacgccga
     4621 tgccgtactc gccggcacgg cagccctgct tgtcctgccc gtccgcgaac cccgaaccgc
     4681 cgcatgacag aatgccgtct gaaaccttca gacggcattt tccaaacacc ccgcccgttg
     4741 ccggactgca cggcacgcaa atcagcctta tcaaaccgca aataaccgcc gcgattaaac
     4801 cgccattcaa ataagcccgt tttcccttcg ggtgtaaaat catcacaaaa cgcgatactg
     4861 tatatcgctg ggcgttatgc agcggatttc aggataaaat gccgtctgaa gccgcccctg
     4921 cccgggcggg cgaagcgtcg gatgcggcgc gcttggcaaa ggtttgcccg ccgcgccccg
     4981 tatccggcat ttcatcaatg cagaccgcat ttccaatcct atattcaagg tgtccgcctt
     5041 atgaaaatcg tttttatcac aacagtcgca tccagcattt acggtttccg cgcccccgtc
     5101 attaaaaaat taatcggcaa aaaccatcag gtgtatgcct ttgtatcgga gttttccgac
     5161 aatgagttgg acattatcag ggaaatgggg gttacacccg ttacctaccg gtcaaaccgc
     5221 agcggggtaa acccgttttc cgatataaaa tccaccttcc tcatatttaa agcactcaaa
     5281 aaaatatcgc cggatttggt tttcccttat ttcgcaaaac ccgtgatttt cggcactttt
     5341 gccgcaaaat tggcaggcgt gcccagaatc gtcgggatgc tggaaggttt gggattcgca
     5401 tttaccccgc agccggaagg cataccgtta aaaacaaaaa tcataaaggg gattttaatc
     5461 gccttgtacc gcattaccct gccgatgttg gaaagcctga ttgtattaaa ccccgacgac
     5521 aaagacgagt tgctgcatca atacggcatc aaaataaaaa acatccatat tttgggcgga
     5581 atcggtctgg atttgcggca atatccttat tccgaggcgg atattcccga tgaaaaagaa
     5641 cccgtaaaat tcctctttat cggcagattt ctgaaagaaa aggggattga tgattttatt
     5701 cgggcggcgg aacaggttaa gggcaaatac cccgatacgg tttttaccgc tttgggcgca
     5761 atcgacaaac cacgcgggag cggggatttg gaacagcctg ccgcccgcga tattatccgt
     5821 ttccccggtt ttgtaaacaa tgtttccgaa gtgataaaag aacatcatat attcgtattg
     5881 ccgtcttatt atagggaagg cgttccccga agcacccagg aggcaatggc cgtcggcagg
     5941 gcggtgatta cgacggatgt ccccggatgc agggaaacgg ttgccgacaa ggtcaacggc
     6001 ttcctgatcg aaccttggaa tccccgcatc ttggccgaaa aaatgattta ttttatcgaa
     6061 aacagggctg ccgtccgcct gatggggaat gcaagttatg cgattgccaa agataaattc
     6121 gatgccgaaa aagtcgattt gaaatttctc gatattttga aggcgtaaac aaggtggtcg
     6181 gcttttgagt ttcggtgatt tctgacaaac tcccgccatt cccgcgaaac cccgtcattc
     6241 ccgcgaaagc gggaatccag gttcgttgag tttcagatgt gtagggcggg ctgtagggtg
     6301 ggcttcagcc caccgaaacc aatcccgcca ccaacctcac cggctcaaac cggcacagca
     6361 acttatatcc cgtcattctc gcgcaggcgg gaatccagcc ccgccggtac agaaacttat
     6421 cgaaaaaaag tttcttcaga ttttacgttc tagattccca ctttcgcggg aatgacggga
     6481 tgcaggtttc cgcgcggacg gattcggcat tcccgctttc gcagaaatgt cggcttttgg
     6541 tttgttgtct ttataagatt attcggtaat tattctgcgg ttttgcgttt gtgtgctgcc
     6601 ggaatctcaa gggattctct gtatttggca acggtgcggc gggaaacctc tataccgcgg
     6661 aaagccagca ggttggcgag tgcctcgtca gaataggact tgggcgtgga cgaggcaaaa
     6721 ccggcggcgg gaaaggggtt gagcgaagca atgaggccga gtgcggcttc gagtgtgccg
     6781 ctgtcggttt ggggcaggcg ttttttattc gcgccggggt ttggctgcgg ttgccgtcaa
     6841 tgctgtcgag ggcgtttcgg acgatatgca gggcggcggg tttggcagca cattcgcccg
     6901 atctttctat ctgcaatatc aacgattcgg tcacatcggc ggcggccatg cctgccggat
     6961 cgaatttttt caatgcggtc agggcttgtt tcagcattgc ttcatccaac atccactcca
     7021 agggcgtatg gtccaggatg tcttcgatgc tgtcggtcag gtagccctgc tcgtcaagga
     7081 aatcgataag gatgtggacg caggcggctt cttggtcgga aagggggtgt tcgcacgctt
     7141 gcgcgtgcag gtattgcttg aaatcttctt cgccggcgat gttggacagc atatcttcgc
     7201 cttcgtctcc gccgatttga cgcgcgggcg cggtgtaatg gctgaattcg gcatcggaaa
     7261 attcatccgt ttctttgcgt tcgagcaggg ggttgtccga cggtcagttt tcgacctcgc
     7321 gttcaagttc gatacccggc atctgcaata cgcgcaaaga ttgttgcagc cgctggtcga
     7381 gctgctgggt ctgtttgagc tttattccga ttaaggtaat gataatgcgg tgaaaattgt
     7441 tattttcagc ctgtcggcgc aaaaaaatgc cgcaaagcgg cattgcatta taaatggttt
     7501 taatgagcgg gttcggattc cgtccgataa caaaaaacaa acgaaaatgg aaacttgatt
     7561 gcttataaca atattaaatc aatttcatag ttttaataac gaaaatcttg gcgtataatc
     7621 gcatccatag tttttgcaaa aggaaaataa aatgtcgatt caagaaattt attacaatca
     7681 agaaaccggt tacgaatacg ctttccgcca aatcgtactg taagccgttt gccccccatt
     7741 tgaccaacct gagaaaagga acaagagcga tgactacctc caaatgcccc gtaacccatc
     7801 tgaccatgaa caacggcgcg cccgttgccg acaatcaaaa cagcctgacc gccggcccgc
     7861 gcggccccct gctgacgcaa gatttgtggc tgaatgaaaa actcgccgac ttcgtgcgcg
     7921 aagtcatccc cgaacgccgt atgcacgcca aaggttcggg cgcgttcggt acgtttaccg
     7981 taacacacga catcaccaaa tacacccgcg ccaaaatttt cagcgaagtc ggcaaaaaaa
     8041 ccgagatgtt cgcccgtttc accaccgtgg caggcgagcg cggcgcagcc gatgcggaac
     8101 gcgacatccg cggttttgcg ttgaaattct ataccgaaga aggcaactgg gatgtggtcg
     8161 gcaacaacac gcccgtgttc ttcctgcgcg acccgcgcaa attccccgac ctgaacaaag
     8221 ccgtcaaacg cgatccgcgc accaatatgc gttccgccac aaacaactgg gacttctgga
     8281 cgctgctgcc cgaagccctg caccaagtta ccatcgtgat gagcgaccgc ggtattcccg
     8341 ccagctaccg ccatatgcac ggcttcggtt cgcataccta cagcttctgg aacgaagcgg
     8401 gcgaacgttt ttgggtgaaa ttccatttcc gcagccagca agggattaaa aacctgacca
     8461 acgaagaagc tgcaaaaatc atcgccgacg accgcgaaag ccatcagcgc gacctgtacg
     8521 aagccatcga gcgcggcgag ttcccgaaat ggacgatgta catccaagtc atgcctgaag
     8581 ccgatgccga aaaagtgcct tatcatccgt ttgacttgac caaagtttgg ccgaaaaaag
     8641 actatccgct gattgaagtg ggcgaattcg agttgaaccg caatcccgaa aacttcttcg
     8701 ccgatgtgga acaatccgcc ttcgcaccga gcaacctcgt tcccggtatc ggcgcgagcc
     8761 cggataaaat gttgcaagcc cgtttgttca attacgccga cgcgcaacgt taccgcttgg
     8821 gtgtgaactt ccgccaaatc cccgtcaatc gtccgcgttg ccctgttcac agcaaccagc
     8881 gcgacggaca aggccgcgcc gacggcaact acggcagcct gccgcactac gaacccaaca
     8941 gcttcggcca atggcagcaa caacccgact tcgccgaacc gcctttgaaa atcaacggcg
     9001 acgcggcaca ctgggactac cgccaagacg atgacgacta cttcagccaa ccgcgcgcct
     9061 tgttcaacct gatgaacgac gcgcagaaac aggctttgtt tgacaacacc gccgcagcca
     9121 tgggcgatgc gcccgacttc atcaaatacc gccatatccg caactgctac cgttgcgacc
     9181 cggcatacgg tgaaggcgta gccaaagccc tcggactgac cgtcgaagac gcacaagccg
     9241 cccgcgcgac cgatcccgca ctgggtcagg ccggtttgct gtaagggggc attatgtgga
     9301 tggaaattga agaaatcctg tcccgcgccg tccgataccg gaaataaccg ggcataaaaa
     9361 tgccgtctga aacgtcgttt gaccgtttca gacggcattt ccacacctcg ccccgccgtt
     9421 tcagtgggcg ttttttatta tagtggatta aatttaaacc ggtacggcgt tgcctcgcct
     9481 tgtcctgatt tttgttaatc cactataaac gcaaaatatc ccgtcattcc cgcaccatcc
     9541 ccaccggttc aaaccggcac gaaaactttt ccgcgtcatt cccgcgaaag tgggaatccg
     9601 gaacgtaaaa tctaaagaaa ccgttttatc cgataagttt ccgtgccgac ggggctggat
     9661 tcccactttc gtgggaatga cgggatttct gtttttgatt ttttgttttt gcgggaataa
     9721 cgggatgcgg gttttcgtgc ggcgtttttc gcgctttgct gttttgttat aatccgccct
     9781 ttttaaggat gggtgcggta tgagtcttta cgctttgctc ttggttgcgc tgggaatgtc
     9841 gatggatgcg tttgccgtcg cgctggcaaa gggtgcggcg gtcaggatgc ccccgcgcaa
     9901 aattgcagca acggctttgg tgttcggtac ggttgaagcg ttcatgccgc tggcgggctg
     9961 ggtaggcggt ttttatgcca agccgtttat cagcgaatga gaccattggg tggcttttgt
    10021 cctgttgggc ggactgggtc tgaaaatgat gcgcgaaggg ttgtccggcg aggcggaaga
    10081 tgtgcgcgaa agcaaacagg aaagcctatg gatgacggtt ttgactgctt ttggaaccag
    10141 tattgattcc atgatcgtcg gggtgggctt ggcgtttatg gaggtaaaca tcgcctttgc
    10201 cgccgccgta atcggtatgg cggctacggt aatggttacc atcgggctga cggcgggaaa
    10261 ggcttttggc gtattgttcg gcaggcgtgc ggaatttgcc ggaggtttgg tgttgattgc
    10321 catcggtaca tggacgctct tatcgcattt gggtttgatt caatgatgtc ggaaaataaa
    10381 atgccgtctg aagctttacc ggtttcagac ggcattttgt taaacttgat tctctatcgg
    10441 tattattgtg taaagctatt gaaaataaaa tattgatttt ccgcaaagat ggctaaaggt
    10501 gcgataggta cactgaagaa ataatcggca gaacaggttt gcctgccggt ttgaacaata
    10561 attttaacct cccataagga atacacgatg tctttcaaac tccgttacct cgcatcagta
    10621 ttggcattgt cttcactgtt ggcggcatgc ggcggtcagg aaaagtctgc ggcaggcgat
    10681 gcttctcctg cttccgagac cgaagcggct tcccaagtac aggcctcgga agccgttcct
    10741 tccgcttcat cggcctcgcc cgaagaccaa gaccttttga aacgcgcgca aggtgtattc
    10801 caacctttgc cgactgtcga agaaatgcag aaaatccgtc cgtttaccga agaacaggtc
    10861 aaactcggac accaactctg gtatgaacca cgcctttcca aaggcaatac cgtaagctgc
    10921 aactcttgcc acaaccttgc ttccgccggt gtggacaata tgccgaccag tcaggggcac
    10981 aaagggcagt tcggcggacg caactcgcct accgcattga atgctgcgct gctgggcagc
    11041 cagttttggg acggacgtgc cgccgatgtt gaagaacagg ctggcgggcc tttggtgaat
    11101 ccggtggaaa tggcgaatga ttcgcaagag gcggctgcag ccaaaatcgc caaagttccc
    11161 gaatatcaag aaatgtttaa aaaagctttt cctgaagacg gcgcggtttc gtttaaaaac
    11221 atcactaccg cattgggtgc gtttgagcgt accctgctga cgccgaccaa atgggacgaa
    11281 tacctcaaag gcaacgtcaa cgccctgagc gaacaggaac gaaaaggcgt gcgcgcgttc
    11341 atggacaacg gctgtattgc ctgccacaac ggtgtcaacc ttggaggcac gaccttccag
    11401 aaattcggtc tggtccaagg gccgtattgg aaattcattg aagatccgaa acgcgacaaa
    11461 ggccgtgctg acgtaaccaa aaaaaccgaa gacgaatttt tcttccgtgt tccggggttg
    11521 cgtaacgtgg ctaaaactta tccgtatttc cacaacggca gcgtgtggga gctggataag
    11581 gcagttacca tcatgggtaa ggcgcaattg ggtaaagaca ttccgaaaga agatgtggat
    11641 aacatcgtcg tattcctgaa tgcactttcc ggcaatgttt ccgaatcagc gcgcacgatg
    11701 ccggaactgc ccctgaccgc accgatggaa tctaagccgg acaacaaata atccggccgg
    11761 tacgcaaaaa tgccgtctga aacgggaatc tgtttcagac ggcattttat atttcgtcgc
    11821 gtcagatacg cagggtatgc cgtagggtgt gcttcagcca gccgtttcca ccaattcaac
    11881 caaccatgtc cgggtcttat gcgtttttca cgaatgcgcg gcgtaggcgg agggattggt
    11941 gggctgaagc ctaattcaat ggctgcgttg tgtaagccat gatgcgtttg gataattaat
    12001 caatattctt gcgctaccat taccatcggc tttgggggtt tgtacttcgg cattatgata
    12061 atcaatagcc tgtttggatt gtattcccga atccgacagt tttagattat aaaaaggccg
    12121 tctgaaaaat tttcagacgg ccttttatag taaatttcaa ataaaaatat ctttgtcgtc
    12181 attcccacgc aggcgggaat ccagacttgt cggcacagaa acttatcggg taaaacggtt
    12241 tcttcaattt tatgttccgg attcccgcct gcgcggggat aacgattcag gtatttctac
    12301 atcgaatccg ctttttatat caacctgact atcaagccgc gttgtcgaag ccgctgtggc
    12361 gcagcagtgc gtctatgctc ggttcgcgtc cgcggaaggc tttgaaggat tccgccgcgc
    12421 tgcgggagcc gccgacggca aggatttcct gccagaagcg tttgcctgtg gcggcgacgt
    12481 cgtcgctttc ttcaaaggcg gcgtaggcat cggcgctgag gacttcggcc catgcgtagc
    12541 tgtaatagcc tgcggaatag ccgccggcga agatgtggcc gaagctgttg gcgaagcggt
    12601 tgtattcggg cggttggatg acggcgactt ctttgcgcac gctgtctaaa acctgctgcc
    12661 agtttttcag acggcattcg tcgctttcac tgtaaatcat catatcgaag agggcgaact
    12721 ccatttgccg gacgaggaac ataccgcgct ggaagttttt ggcggcgagc attttgtcga
    12781 agagttcttt cggcaggggc tcgccggttt cttcgtgggc ggacatttgt gccaatacat
    12841 tgtattccca aacgaagttt tccataaact ggctgggcag ttcgaccgcg tcccattcta
    12901 cgccgttgat gccggacacg cccagttcgt ccacttgggt aagcaggtgg tgcagtccgt
    12961 ggccggtttc gtggaagagg gtgaggattt cgtcgtggct taaacgcgct tctttgccgc
    13021 cgacgggcgg ggcgaagttg cagacgaggt aggcggtggg cagttgcagc gtgccgtcgg
    13081 caaagcggcg gcggcctttg tagtcgttca tccacgcgcc gccgcgtttg ccttcgcgtg
    13141 cgtacaaatc catataaacg ccgccgatgg tttcgccgtt ttgttgcaat tcaaaatagc
    13201 gcccgtcttt gtgccagacg gggacggttt tttcggcaaa tccgatgccg tagagttttt
    13261 tgatttgggc gaacagtccg tttaatactt tgccgacggg gaagtatttt ttgacttcgg
    13321 tttcgctgaa tgcgtatttg gcttcgcgca gtttttcgcc ggcgtagctc aagtcccacg
    13381 gctgcgggtc ggcgagaccg aggtgttcgc gggcgaaggc tttgacttcg gcgaggtctt
    13441 tttcggcgta gggtttggcg cggcgggcga ggtcgtgcag gaagtttaaa acctgttcgg
    13501 gcgtgtccgc cattttggtt gccagcgaca attcggcgta atttttaaag ccgagcagtt
    13561 tggcggtttt caatgcgttt tcgagcgtgc ggtcgatgtt ggcggtgttg tcgaatttgc
    13621 cgtcgtttga aagttcgctg gcacgggtaa cgtaggcgcg gtagatttgt tcgcgcagtt
    13681 cgcggttgcc ggcgtattgg atgacggcaa ggtagtgcgg aatctgcaag ccgattttgt
    13741 aacctgtttt gccttcgctt tgcgcggcgg cggcaaacat cgcgagcgcg tcttcgggaa
    13801 tgccggcaag cggtgcggca tcgtcaaagt aaatgccgaa cgcgtcggtc gcgtctagga
    13861 cgttttggga gaatttggcg gaaagttgcg cgccttcggt ttgcagtttt gccagttctg
    13921 cctgccgttc gggcggcagt tccgcgccgc tcaatacgaa atcgcgcagg tcgtgatcga
    13981 gcttggtttt ttgtgcgggg gaaagcgttg caaattcggg ggaatttttg atggttttga
    14041 agcggttgta cagttcgatg tcttgtccga tttcggtgaa gaagacggtg atttcaggca
    14101 tcagttcgtt atagacggcg cgcagttcgg gcgtgtcgac gacggagttg agatgggaca
    14161 cgacgcccca aatcctgccg acgcgttcgg tgatgccggt cagacgctcg acggtgttcg
    14221 cccagccggt gtgcgtttgc gctttgacgg cggcgatttg tccgcgcgct tcggcgatgg
    14281 cggtttggac ggcgggtttg atgtcttcgg ttttgatttg attaaaacgg ggttcttcgc
    14341 ccaagtggag cagtgcgttg tcaatcataa gatgggtttc ctttgcgtgt tgtgcttcag
    14401 acggcatatc ggcaatatgc cgtctgaagc cagaaatggg gccggccggg gaaagttaaa
    14461 gtgtttcgga gccggcggga tgatttaaca ggcggtattg tacgcgcaaa aattcgtcca
    14521 taaccgcctg ttggatttcc agccgctttg aaacggggga ggcgaagcgg acgatgatgc
    14581 ggtatgcctt gtcgtcgtac ggtacgcggg taacgcgcgg cctggcggcg ggcgtgataa
    14641 acagtttttc cgcctgcacg ttttccaaat accgctgaat ggcggggatg tagggcgcgc
    14701 acaagggctc gagtacggct ttcagacggc atacggcttc atccgaatcc aaatggatgg
    14761 gaacggggat ttcgaccgta tggatgacat agtcgcccaa aatattgtcg cggcgcacgg
    14821 ggtggctcaa caacaggctg ttggggaaag aaacggtggt tcccgcaagc tgtccgacca
    14881 aggggttcgg accgacctgc atcatcagcg tgttcaacag attgatgtcg accacgcgcc
    14941 cgcgcaggcc gttgatttcg atatagtcgc cgaccgagta ttgctgggtg gcagacctta
    15001 aaatactgcc cgacagacac ataatcagtt cttttgtcgc cacgacgacc gccgccgcca
    15061 ccgcaaacat cgacaaagcc agcgtttgaa tttgcgccga ccagataaat gccagcgaaa
    15121 acagcaccaa aagcagcgtt atattgcggc tggcaaccaa aaaccgccgc ttgctttcga
    15181 tgccgaaatc cggatgccgt ctgaagtgga tattcaacag aagggcgcgc gccagcagca
    15241 aagccgcgac cgccgccacg gattcggccg cctccgcgcg tatcgggacg gcatcgggcc
    15301 aagtgttcaa catattccat atttccattt ccgcgcccct gtccgaaaag tatagggtgt
    15361 agattttagt ggcaaaaaaa cgcttttgcc actttgccgg caaattccgc gcccgtaccg
    15421 gccgcaaatg cgccgcacgc cctttcagac ggcatccgga tgcggataac gctgaatcag
    15481 gcggcgaaat cggctataat acacaattat atgcgccgtc ccgcccgctt tttccacaag
    15541 gtgcggactg ttttggcggc atcgcaaaat cttttcaaaa tccggcaaaa aatatgactg
    15601 aacaaaaaca cgaagaatac ggcgccgaca gcatccaggt gctcgaaggc ttggaagcgg
    15661 tacgcaaacg ccccggcatg tacatcggcg acacgcagga cggcagcggc ctgcaccata
    15721 tggtgtttga agtattggac aacgccatcg acgaagcact cgccggacat tgcgacaaaa
    15781 tcacggtaac gatacacgcc gaccattccg tcagcgtcgc cgacaacggg cgcggtatgc
    15841 ccaccggcat ccacccgaaa gaagggcgtt ccgccgccga agtcatcatg accgtcttgc
    15901 acgcgggcgg caaattcgac aacaacagct acaaaatctc cggcggcctg cacggcgtgg
    15961 gcgtatccgt cgtcaacgcg ctgtccgact gggtaacgct gaccatctac cgcgacggca
    16021 aagaacactt cgtccgcttc gtacgcggcg aaaccgaaga gccgctgaaa attgtcggcg
    16081 attccgacaa aaaaggcacg accgtgcgct tcctcgccgg cacggaaacc ttcggcaata
    16141 tcgaatacag cttcgacatc ctcgccaaac gtattcgcga actttcgttc ctaaacaacg
    16201 gcgtggacat cgaattgacc gacgagcgcg acggcaagca cgaaagcttc gccctttccg
    16261 gcggcgtggc gggcttcgtg caatacatga accgcaaaaa aacgcccttg cacgaaaaaa
    16321 tcttctatgc gttcggcgag aaagacggca tgagcgtcga atgcgcaatg caatggaacg
    16381 acagctatca ggaaagcgtg cagtgcttca ccaacaacat ccctcagcgc gacggcggta
    16441 cgcacctgac cgcgctgcgc caagtgatga cgcgcaccat caacagctac atcgaagcta
    16501 acgaagtcgc caaaaaagcc aaagtggaaa ccgccggcga cgatatgcgc gaaggtttga
    16561 cctgcgtgtt gtccgtcaaa ctgcccgacc ccaaattctc atcccaaacc aaagacaaac
    16621 tggtttccgg cgaaatcggc cccgttgtca acgaagtcat caaccaagca ctaaccgact
    16681 tcctcgaaga aaatccgaac gaagccaaaa tcatcaccgg caaaatcgtc gatgccgccc
    16741 gcgcacgcga agccgcccgc aaagcccgcg aaatcacccg ccgcaaaggc gtgatggacg
    16801 gcttgggact gcccggcaaa ctcgccgact gccaagaaaa agaccctgcc ctgtctgaac
    16861 tctacctcgt cgagggcgac tccgcaggcg gttccgccat gcagggccgc gaccgcaaat
    16921 tccaagcgat tttgccgctc aaaggtaaaa ttttgaacgt cgaaaaagca cgttttgaaa
    16981 aaatgctcgc cagccaagag gtcgccaccc tgattaccgc gctgggtgca ggcatcggca
    17041 aagaagagtt caaccctgaa aaactacgct accaccgcat catcatcatg accgatgccg
    17101 acgtggacgg tgcgcacatc cgcaccctgc tcctgacctt cttctaccgc caaatgcccg
    17161 aactggtcga gcgcggctac atttacatcg cccagccgcc gctctacaaa gccaaatacg
    17221 gcaagcagga gcgttacctc aaagacgaac tggaaaaaga ccaatggctg ctcggccttg
    17281 ccttggaaaa agccaaaatc gtttcagacg gccgcaccat cgaaggcgca gaacttgccg
    17341 acaccgccaa acaattcttg ttggcgaaaa ccgtcatcga acaggaaagc cgcttcgtgg
    17401 acgaactcgt cctgcgtgcc atgctgcacg cgtcgcccat tgatttgacg tcgtctgaaa
    17461 acgccgataa agccgttgcc gaactttccg gtttgcttga cgaaaaagaa gccgccctcg
    17521 aacgcatcga aggtcatgaa ggacaccggt tcatcaaaat cacgcgcaag ctgcacggca
    17581 acgtcatggt cagctacatc gaacccaagt tcctcaacag caaagcctac caaaccctca
    17641 cccaaaccgc cgccgcgctc aaaggcttgg tcggcgaggg cgccaagctc tacaaaggcg
    17701 agaacgagta cgacgcggac agctttgaaa ccgctttgga catcttgatg agcgttgccc
    17761 aaaaaggtat gtccatccaa cgatacaaag gtttgggcga gatgaacccc gagcagcttt
    17821 gggaaaccac gatggatccc gccgtgcgcc gcctgttgaa agtgcgcatc gaagatgcca
    17881 ttgccgccga cgaagtgttc gttaccctga tgggcgacga ggtcgaaccg cgccgcgcct
    17941 ttatcgaaaa caatgcgctg attgcgcaaa atatcgacgc ataagtgccg ttttaaaaaa
    18001 ggagacgggc agcttgccgc gtctcctttt ggtttgtcaa acggaagcca tgccgtctga
    18061 aaccgccttt cggagcaaaa ccatgatcag catttttgat attttcaaaa tcggtatcgg
    18121 cccttccagt tcgcatacgg tcggcccgat gaaggccgcc gccgcctttg ccgcaggctt
    18181 ggacgcgcaa accgcccgca tcgccatcga catttacggc tcgcttgccc tgaccggata
    18241 cggacacggc acatttgacg cgctgatgct cggtttggaa ggcagcctgc cgcacgacat
    18301 cccgcttgcc ggcattcccg aacgcctcgg acgcatccgc acgcagcaca tcctccggct
    18361 caacgggcaa gaaatccgct tcatccccga ccgcgacctg aacatacgcg gcaatcaagt
    18421 gctgcccaaa caccccaacg gcctgcgatt taccgcctat gcttcagacg gcacggtatt
    18481 gaaggaacag gtttattatt cggtcggcgg cggctttgtc gttaccgaag aagacttcga
    18541 gtggcagtcg gaaacggaaa aagccgttcc ttatccttac aacagttgtg ccgggctgct
    18601 tgcccgatgc cgtctgaacc ggctcgacat ctccgaagtc gtgttggcaa acgaagccgc
    18661 gcttgccgga tgcggcgaag ccgaaatccg ccgccgcgtc gctgccgttg ccgaggttat
    18721 ggaaggctgc atcaaacgcg gcttgggtgc ggacggcgaa ctgcccggcg gattgaacgt
    18781 ccgccgccgc gccccgcagc ttgccgccaa actcaaagtc ctgcgcgaaa ccgaaatcgt
    18841 caacacccag ctctggccga tggtgtacgc catggcggtc aacgaagaaa acgccgccgg
    18901 cggacgcgtc gttaccgccc cgaccaacgg cgcggcaggc atcattcccg ccgtattgca
    18961 ttatttccgc aagttcaacc cccacgccac acaggaacgt gtcgaaaact tcctgctcac
    19021 cgcaggcgca atcggcatcc tctacaaaac caacgcctcc atttccggcg cggatgtcgg
    19081 ttgtcagggc gaagtcggcg tagcgtgttc aatggcggcg ggtgcatacg ccgaagtcat
    19141 cggcggcacg cccaaacaag tggaaaacgc cgccgaaatg gcaatggaac accatttggg
    19201 cttaacttgc gaccccgtcg gcgggttggt gcaaatcccc tgcatcgaac gcaacggcat
    19261 cgccgccgaa aaagccctca aactcggcac gctcgcgctc ttggaagacg gcacggacaa
    19321 aaaagtctcg ctcgacgaag tcatccgaac catgctgcaa accggccggg acatgaaggc
    19381 gacctacaaa gaaacctcgc ttgccggact cgccgccacg ctccggaaaa aagccgtccc
    19441 cgtatccgtg cgcgtggtcg agtgctgagg atggacagaa acgaaaatgc cgtctgaaaa
    19501 cagtttttca gacggcattt catttatttt ttgcgggttt accgcttcaa cgccaacgtc
    19561 aacacgcccg ccgtaaccag ccccaagcct atccattcct gcgtgttcgg gcgttcgtcc
    19621 aagaaaacca ccgccatcag gaatgcaaac cggcgttgtt tgcagcatcc ggcaagaatt
    19681 tttatagtgg attaacaaaa atcaggacaa ggcg
//
LOCUS       DAXDXW010000038        19711 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXDXW010000038 DAXDXW010000000
VERSION     DAXDXW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19711
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.039"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            65..670
                     /locus_tag="ACFB64_001153"
     CDS             65..670
                     /locus_tag="ACFB64_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC1209517.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDIDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAAANKEQAKAREAIGNGLTMLSPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            742..1134
                     /locus_tag="ACFB64_001154"
     CDS             742..1134
                     /locus_tag="ACFB64_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC1209518.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLRIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     EYRPDTPSENTSAETAPRPAKKGLKLVK"
     gene            1139..1264
                     /locus_tag="ACFB64_001155"
     CDS             1139..1264
                     /locus_tag="ACFB64_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209519.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNLFRRIETVLPLPIRY"
     gene            complement(1333..1779)
                     /locus_tag="ACFB64_001156"
     CDS             complement(1333..1779)
                     /locus_tag="ACFB64_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC1209520.1"
                     /translation="MSLKTRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDGAKIYTEAGRQDLADKENAEIDVLHRYLPQMLSAGEIRTA
                     VEAAVAETGAAGMADMGKVMVVLKTRLAGKADMGEVNKILKTVLTA"
     gene            complement(1911..2123)
                     /gene="rpsU"
                     /locus_tag="ACFB64_001157"
     CDS             complement(1911..2123)
                     /gene="rpsU"
                     /locus_tag="ACFB64_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC1209521.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(2412..4262)
                     /gene="ltgA"
                     /locus_tag="ACFB64_001158"
     CDS             complement(2412..4262)
                     /gene="ltgA"
                     /locus_tag="ACFB64_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC1209522.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQNNDAAAAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLKPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSPEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFRNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLAAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEILATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYASLFGAPHIPLKQRMGTVPAR"
     gene            4639..5376
                     /locus_tag="ACFB64_001159"
     CDS             4639..5376
                     /locus_tag="ACFB64_001159"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC1209523.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIQDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLSNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHAQSEIGRELIRED"
     gene            5378..6064
                     /locus_tag="ACFB64_001160"
     CDS             5378..6064
                     /locus_tag="ACFB64_001160"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC1209524.1"
                     /translation="MADLTFEQAVSTIVGMKDEIVRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVLKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            6223..7089
                     /locus_tag="ACFB64_001161"
     CDS             6223..7089
                     /locus_tag="ACFB64_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC1209525.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASAAAPSADNGAAKK
                     EIVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHK
                     PYLDDFKKEHNLDITEAFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARALV
                     MLNELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYA
                     ISSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGY
                     KYPAAWNEGAAK"
     gene            complement(7152..7721)
                     /locus_tag="ACFB64_001162"
     CDS             complement(7152..7721)
                     /locus_tag="ACFB64_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC1209526.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDVETHLVVSKGAEMAR
                     ASETDYTKDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSIAHTLSLFGIDTPDLAEWQGMAD"
     gene            7879..8739
                     /locus_tag="ACFB64_001163"
     CDS             7879..8739
                     /locus_tag="ACFB64_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC1209527.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTAVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLSEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPESVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            8875..9051
                     /locus_tag="ACFB64_001164"
     CDS             8875..9051
                     /locus_tag="ACFB64_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209528.1"
                     /translation="MNTRINFTATVGKRVSQCYVTSVISTIGVTTSDAICSGGGTNKA
                     KSQCNALLKAAGRC"
     gene            9414..9767
                     /locus_tag="ACFB64_001165"
     CDS             9414..9767
                     /locus_tag="ACFB64_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC1209529.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFVPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            9757..10623
                     /gene="atpB"
                     /locus_tag="ACFB64_001166"
     CDS             9757..10623
                     /gene="atpB"
                     /locus_tag="ACFB64_001166"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC1209530.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHSEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRIVPTADLNTALALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            10680..10916
                     /gene="atpE"
                     /locus_tag="ACFB64_001167"
     CDS             10680..10916
                     /gene="atpE"
                     /locus_tag="ACFB64_001167"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC1209531.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            10986..11456
                     /locus_tag="ACFB64_001168"
     CDS             10986..11456
                     /locus_tag="ACFB64_001168"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC1209532.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKIAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLRDQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            11461..11994
                     /locus_tag="ACFB64_001169"
     CDS             11461..11994
                     /locus_tag="ACFB64_001169"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC1209533.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ALLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            12005..13552
                     /gene="atpA"
                     /locus_tag="ACFB64_001170"
     CDS             12005..13552
                     /gene="atpA"
                     /locus_tag="ACFB64_001170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC1209534.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVSTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTLGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPVEKIAPGVIARKSVDQPMQTGLKAIDSMIPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            13577..14452
                     /gene="atpG"
                     /locus_tag="ACFB64_001171"
     CDS             13577..14452
                     /gene="atpG"
                     /locus_tag="ACFB64_001171"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC1209535.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            14490..15887
                     /gene="atpD"
                     /locus_tag="ACFB64_001172"
     CDS             14490..15887
                     /gene="atpD"
                     /locus_tag="ACFB64_001172"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC1209536.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGSPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFSGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LAVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            15898..16320
                     /locus_tag="ACFB64_001173"
     CDS             15898..16320
                     /locus_tag="ACFB64_001173"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC1209537.1"
                     /translation="MSIMQVEVVSGEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGILEVQPDKVTVLADVAVRSAEMDRARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            16492..17388
                     /gene="glyQ"
                     /locus_tag="ACFB64_001174"
     CDS             16492..17388
                     /gene="glyQ"
                     /locus_tag="ACFB64_001174"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC1209538.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLFAEENADLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYIESREKLGFPLIKK"
     gene            17505..19568
                     /gene="glyS"
                     /locus_tag="ACFB64_001175"
     CDS             17505..19568
                     /gene="glyS"
                     /locus_tag="ACFB64_001175"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC1209539.1"
                     /translation="MTTQTLLIELLTEELPPKALNNLGNHFAASVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGTPTKALEGFARGAGAK
                     IEDLTIIHDGRQDVYAYEYVQTGRPLGGLLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDVVNVSVLGLQSGNQTLGHRFLSDGEMIIENADSYAAQMRGQGKVVA
                     SFAGRKAAIQTALEGQARRLNATVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPQGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     VFKQLPEAAALAAANKRVQNLLKKADAALGAVNESLLQQDEEKALYAAAQGLQPKIAA
                     AVAEGNFRTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            19632..>19711
                     /locus_tag="ACFB64_001176"
     CDS             19632..>19711
                     /locus_tag="ACFB64_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC1209540.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLAAVV"
BASE COUNT         4657 a         5257 c         5186 g         4611 t
ORIGIN      
        1 ggtttaaatt taatccacta taaatcagcc gttttgcagg catcacacag gagcgacaat
       61 tattatgatg accctctatt ccggcattac ctgccccttc agccaccgct gccgcttcgt
      121 tttgtacgaa aaaggtatgg attttgaaat caaagatatc gatatttaca acaaacccga
      181 agacctcgcc gtcatgaacc cgtacaacca agtccccgtg ctggtcgagc gcgatttggt
      241 gctgcacgag tccaacatca tcaacgaata catcgacgaa cgcttccccc atccgcagct
      301 tatgcccggc gatcccgtta tgcgcggccg ggggcggctg gtgctgtacc gtatggaaaa
      361 agaattgttc aaccacgtcc aagttttgga aaaccccgcc gccgccaaca aggaacaggc
      421 aaaagcgcgc gaagccatcg gcaacggcct gacgatgctt tccccttcgt tcagcaaaag
      481 caaatacatc ctcggcgaag atttttctat gattgatgtt gccctggccc cgctgttgtg
      541 gcggctcgac cattacgatg tcaaactggg caaaagtgcc gcgccgctgc tcaaatacgc
      601 cgagcgtatc ttccagcgcg aagcctttat cgaagcactg acacccgccg aaaaagccat
      661 gcgcaaataa gtccgaaatg cttgcaaaac ccgccgtttt gcaggcattt tcacattcag
      721 gcacaccaca cggaacccat catgcccacc tcaaccaaac cctacatcct ccgcgcactt
      781 tgcgaatggt gcagcgacaa cagcctcacg ccgcacatcc ttgtctgggt aaacgagcat
      841 acgcgcgtcc ccatgcagta cgtccgcgac aacgaaatta tgctcaacat cggcgcaacc
      901 gccacgcaaa accttcgaat cgacaacgat tggatcagct tttccgcccg tttcggcgga
      961 caagcgcacg acatatggat acctgtcgga cacgtcctca gccttttcgc acgggagacc
     1021 ggagaaggta tggggtttga attggaggag taccgccccg acacgccgtc tgaaaacact
     1081 tctgccgaaa ccgcgccccg gcccgccaaa aaaggtttga aattggtcaa ataaatctat
     1141 gccgtctgaa cggaatcgtg tttcagacgg cattttgtcc gatggggcgc aaacggaatc
     1201 cgtttatcgg caaaacctgt ttcggcgtat cgaaaccgtg ttgcccctgc cgattcgata
     1261 ttaggacatt gaaaatgccg tctgaacctg cgatacgggc ttcagacggc gttttgtccg
     1321 atattcgggc aatcaggcgg tcagtacggt tttcaagatt ttgttgactt cgcccatatc
     1381 ggctttgccg gcgaggcggg ttttcaatac gaccatcact ttgcccatat ccgccatacc
     1441 tgccgcgccg gtttcggcaa cggctgcttc gacggcggtg cggatttcgc cggcggagag
     1501 catttgcggc aggtagcggt gcagcacgtc gatttcggcg ttttctttgt ctgccaaatc
     1561 ctgacggccg gcttcagtgt agattttcgc gccgtctttg cgctgtttga ccattttggt
     1621 caggatggcg gtgattttgg catcgtcggc ttcggtgcgt tcgtctactt caaactgttt
     1681 gacggcggca ttgatgaggc ggatggtgcc gagggaaact tgatctttgg cgcgcatcgc
     1741 ggttttcata tcttcggtaa ggcgggtttt caggctcatg atgtcctcgc tgggatgtcg
     1801 gatggaaacg gcgggtttcg atgccgtctg aaaagcaaaa cacaccgcag agcaggtctt
     1861 gcggtgtgtc ttcatatcac agggctgacc tgtaatctgt acttgaacgt ttagtacatt
     1921 ttgggcggca gctgttggct gcgcaggcgt ttttgcaggc gttttacggc tgccgctttt
     1981 ttgcgtttgc gttcggtagt cggtttttcg taggcttcgc gggcgcgcag ctcggtcagc
     2041 aggccggttt tttctacggc gcgtttgaaa cggcgcatag cgacttcaaa tggttcattc
     2101 tcttttacgc ggattgcagg cattttattt cctttaataa attcggttgt tccatctgcc
     2161 catcatatcg gtggaaaggg tagggcagac ggtgttgtag gttttccgta tcgctgtgcc
     2221 tttcggcggc gggatacgcg ccgtgacgga aacatcacct cctaacgggc cggcttgtgc
     2281 cgtgtcaagc tgcttgaaaa agtaggaaaa acaattttcg gattgtctta tatttatggt
     2341 tcgccgtcaa tgccgttcgg gataaaaatg ccgtctgaaa gaccgggcgg gtttcagacg
     2401 gcatcggcac gtcagcgtgc gggaacggtg cccatacgct gtttgagcgg gatgtgcggc
     2461 gcgccgaaga gggaggcgta gtaggcggca ttggtcatca cttttttgac atagtcgcgc
     2521 gtttcggaaa acgggatggt ttcggcatat accgcgcctt cgaggggtgt gtccgcctgc
     2581 catcggcgcg ccctgccggg gccggcgtta tagcctgcgg tggcgaggat ttcgttgttt
     2641 tgcaggcggc gtttggtgtc cgccatatac cacgtcccca tacggatatt gccgtcggcg
     2701 gtgtagagct gggcggcatc cataccgatt ttgccggcga tttcgcgcgc ggtggcgggc
     2761 ataacctgca tcagcccctg cgcgcctacg cgggattgcg cgcctataac gaagcggctt
     2821 tcctgacgaa tcaggccgta aacccaagcc ggatcgacat taacattttg cgcgtggcgg
     2881 attaccgtgt ctttaaacgg cgaaatatag cgcaaggtgt agttgagttt gcggtcggtg
     2941 cgttccgcgc tgttgaccgc catatcgtaa aaaccgtggt cgaacgcggt ttgcgcggcg
     3001 gccagcagct tgtcttcgtc aaagccgcgt gtggcaaaac gccattccgc ctgagcctga
     3061 cggcgcattt tcgcatcgcc ggcggttcgg ctgtttcgga acagcaccag cgcgcgctta
     3121 atcgcgccgt cttccgccat acggaggaca ctgtttttgc cggcatcggg cacattgttg
     3181 cgcgtatcga ttttgcgccc caattcttcc cctgccagta cggcataaaa attcctgccc
     3241 gtcgctgccg cctgtttgta aagtttttcc gcttcttgcg tgttgcccgt tgcggcgcgg
     3301 ctgcgtgcca gccagtagag ccaggtcggg cttttttgca gtttttcggg catatgcgag
     3361 atcacggagg ccagctcgtc ccaacgtcgg gcgcgcaagg cggcgcgggc gtaccactcg
     3421 atttggtcgt cggtcagttg gcggcggtcg gcaaccttgc cgtaatagtc caaggcggca
     3481 ggcacattga ggctttgcga ctgataatgc cccaatacgc cccacgcgaa actgcgttgt
     3541 tcggggctta aaccgctttc catttcggac agcagggcgg cggcattcgg cgatttgcgt
     3601 gcttctttgc cgatgacgtt caacagggca tattcgcgcg aaccttgtgt accgccgtca
     3661 aacgggctgc ccaatgcggc ggcaaggttg cgcgcgtccg tcgtttggcg gccggccagc
     3721 agtccgcgca cgcgcctcca ggcatcgttg ccgtccaaca agccggatgc ggctgcctgt
     3781 tccaacagtt tggtgcagcc cgaaggcagt ttgcccgtat ttttgaccag ttcggcggca
     3841 cgcgtatagt cgttgcggct cgaatcggcg tagcattcga cttcttgggc gcgcccttca
     3901 ggtttgagtt tggcatattc ctgtgcaaac agcgtccact gtctgcgcgc gcccaaagac
     3961 ttcagccact cgttgcggac attttccgcc atcgcgctgt cgccggcgtt ttccaaatag
     4021 gcggcggctg ccgcatcgtt gttctgtttc actgcatcca gtgcggacgg gtagccgccg
     4081 taatctgcca gcgtttttcc ttccggttcg gcagggcggg tgggaacgct tgccgaaagg
     4141 tcggcagttt ctatattgtc tgccggggtc ttgccggctg gcagtgtgtt tgtcgaagaa
     4201 cacgcggcaa gcaccagggc cgccagcagc ggcagggaat gcttcataga gggtaggtac
     4261 atcggatttc cttaagaatc ggaaccctga acggtcaggg ttggaaaaga caaaatgccg
     4321 tctgaacagg cgtttgcccg aattatatgc cgaaactgca ccgtctttgg aatgtttccg
     4381 acataattta tatttttcaa tcatttgccg tttgggtgcg aaccgctgcc tttgcccgtt
     4441 tcagacggca ttgtccgaaa tggttgcccg cttcctgcct tattgacaaa aaaacgcttt
     4501 cccgataata tcctacgaaa attaacctgc cgatttgaca cagcttgcgg gcataacagc
     4561 taaagcgttc cgacaatttc agctttatct tccgcgcccg ttgtgtccga catcgggctt
     4621 tgttgtatgg gaaagacaat gattattttg gacaaggttt ccaagcatta ccaaacgcgc
     4681 gacaaaaccc gttttgccgc cgtcgagccg accagcctcg aaattcaaga cggcgaaatc
     4741 ttcgggctga tgggctattc cggcgcgggc aaatccactt tgttgcgcct gatcaacctg
     4801 ttggaacgcc ccgacacggg caaagtcaac gtctgcggac aagaactgac cgcgctcgat
     4861 gccgccgcat tgcgtcaggc gcggcagaat atcggcatgg tgtttcagca gttcaacctt
     4921 ttaagcaacc gcaccgtcgc cggcaacgtt gcctttccac tggaaatcgc cggatggccg
     4981 tctgaaaaaa tcaaagcgcg cgttgccgag tgcctcgaaa tcgtcggctt gaccgaacgc
     5041 gccggccact accccgccca gctttcaggc gggcagaaac aacgcgtcgg aatcgcccgc
     5101 gcgctcgcgc ccaagccgca agtcatcctc gccgacgaac ccacttccgc cctcgacccc
     5161 gccaccacgc gcagtgtctt ggaatgtttg gaagacatca acaaacgctt caacgttacc
     5221 atcgtcatcg taacccacga aatgagcgtc atcagacgat tgtgcgaccg cgccgccctg
     5281 ttggataaag ggaaagtggt ggaaatcgtc gaagtacgcg gcaaccaaat ccacgctcaa
     5341 tccgaaatcg ggcgcgaact gattcgggag gactgatatg gcagacttaa cattcgaaca
     5401 agccgtttcc accatcgtcg gcatgaaaga cgaaatcgtc cgcgccttgg gcgaaacttt
     5461 cgtgatggtc ggcttgtcca ccacattcgc cgtcatcttc ggcacgctct tgggcgtgtt
     5521 gctcttcgta acttccagcc gccaactgca ttacaacaag ccggtgaact tcctgctcga
     5581 caacctcgtc aacctcatgc gcgccttccc cttcgtcatc ctgatgattg cgatgatacc
     5641 cgccacacgc gccatcgtcg gcagcaccat cggcccggtt gccgcctcgc tggtgttgag
     5701 cgtgtcggga ttgttttatt ttgcccgact ggtggaacaa aacctgcgcg aagtcccaaa
     5761 aggtgtgatt gaagccgccg ccgcgatggg tgcgccgccg attgccatcg tcctcaaagt
     5821 cctcttgaac gaagcgcgcg cgggcatggt ttccagcatt accgtgcttg ccatcgggct
     5881 tttgtcatac agcgcggcgg caggcatgat aggcggcggc ggcttgggcg acctcgccat
     5941 ccgctacggc tactaccgct accaaaccga agtcatcgtc ttcatcgtcg ccctcctcgt
     6001 gctgctggtc atcctgattc aaagcaccgg caacgcgttg gcgcggaaac tcgacaaacg
     6061 ttaaacccga atgccgtctg aacgccaaaa accccgccgc tatccgaaaa atgctataaa
     6121 atccccctgt tcgcggcaaa tgccgtctga acgccgaatc cggacggcag gactccctgc
     6181 ctgtcatttt tgtttgaaac cgccacaaca tcaggagaaa atatgaaaac cttcttcaaa
     6241 accctttccg ccgccgcact cgcgctcatc ctcgcagcct gcggcggtca aaaagacagc
     6301 gcgcccgcag cctctgccgc cgccccttct gccgataacg gcgcggcgaa aaaagaaatc
     6361 gtcttcggca cgaccgtcgg cgacttcggc gatatggtca aagaacaaat ccaagccgag
     6421 ctggagaaaa aaggctacac cgtcaaattg gtcgaattta ccgactatgt gcgcccgaat
     6481 ctggcattgg cggagggcga gttggacatc aacgtcttcc aacacaaacc ctatcttgac
     6541 gatttcaaaa aagaacacaa cctggacatc accgaagcct tccaagtgcc gaccgcgcct
     6601 ttgggactgt atccgggcaa actgaaatcg ctggaagaag tcaaagacgg cagcaccgta
     6661 tccgcgccca acgacccgtc caacttcgca cgcgccttgg tgatgctgaa cgaactgggt
     6721 tggatcaaac tcaaagacgg catcaatccg ctgaccgcat ccaaagccga catcgcggaa
     6781 aacctgaaaa acatcaaaat cgtcgagctt gaagccgcac aactgccgcg cagccgcgcc
     6841 gacgtggatt ttgccgtcgt caacggcaac tacgccataa gcagcggcat gaagctgacc
     6901 gaagccctgt tccaagagcc gagctttgcc tatgtcaact ggtctgccgt caaaaccgcc
     6961 gacaaagaca gccaatggct taaagacgta accgaggcct ataactccga cgcgttcaaa
     7021 gcctacgcgc acaaacgctt cgagggctac aaataccctg ccgcatggaa tgaaggcgca
     7081 gccaaataag gcagtcgtat aaaatgatgc cgtctgaacc gcattccgtg ttcagacggc
     7141 atttttgtcc tttaatccgc cattccctgc cattccgcca aatccggcgt atcgattccg
     7201 aacagcgaca gcgtgtgtgc gatactgtgc gccactatgt cgtccgccgt ctgcggcttg
     7261 cggtacatcg caggaacagg gggaaacacc acgccgccca tttccgttac ccgcttcata
     7321 ttgtccaaat gggcaaggtt cagcggcgtt tcgcgcacca tcagcaccag ccgccgcctt
     7381 tccttcaaaa ccacatccgc cgcacgcgtc aagagattgt cgccgaagcc gtgcgcgaca
     7441 gaggcaagcg tccgcatcga acagggtgcg accagcatcc cgtccgtttt aaacgtaccg
     7501 ctggcaatgc acgccccgat attgccgatc ggatggacga aatcagccaa ggcatatact
     7561 tcgtctttcg tataatccgt ttccgaagcg cgcgccatct ccgcgccttt cgataccaca
     7621 aggtgcgttt cgacatcttg cgcgcgcaaa agttccaaag ccttcacgcc gtattggaaa
     7681 ccgctcgccc cgctgatgcc gattatcaaa cgccgtacca tcatccgcct ttcccataaa
     7741 accgcctgca acggcaaacc ggctattata gtgaaaaaac agaaatccga taaacacgga
     7801 tacaaattgt cggcaacacc caatatccga taaaataccc gatttaacat cctatctgaa
     7861 taggcacggg agggcggtat ggcaaaagta aaaggcggat tggggcgcgg cttggattcg
     7921 ctgctcgcca acggtgcgga caacagcagc ggcgaccggc tgactgcggt tgcggttaaa
     7981 gatatccggc ccggccgcta tcaggcgcgc gtccaaatcg atgacgaagc cttgcaggaa
     8041 ctggcagatt cgattaaggc gcaaggcgtg atacagcccg tcatcgtgcg cgaacacgga
     8101 ctgtcccgat acgaactgat tgcaggcgaa cgccgttggc gcgccgctca gattgccggc
     8161 ctgtccgaaa tccccgccgt tatcaaaacc atcagcgacg aaaccgcatt ggcaatgggt
     8221 ttgatcgaaa acctccagcg cgaaaacctc aaccccatcg aagaagcaca aggcttgaaa
     8281 cgccttgccg acgagttcgg gctgacccac gaaaccatcg cccaagccgt cggcaaaagc
     8341 cgaagcgcga tttccaacag cctgcgcctt ttaagcctgc ccgaatccgt gcaggaaatg
     8401 ctttaccaac gccgcctcga aatggggcac gcccgcgcac tgctgaccct gcccgtcgtc
     8461 gaacagctcg aattggcgca aaaggccgtc aaaaacggct ggtcggtgcg cgaagtcgaa
     8521 cgccgcagcc aggccgccct tcaaaacaaa cgtcccgagc caaaaaaaac tgccgccgcc
     8581 gacatcggcc gcctgaatga tttgctgact gaaaaattgg gtgtcaacgc cgaaatcaag
     8641 accgccaacc ataaaaaagg caaaatcatc ctgcatttcg acacgcccga aaccttcgac
     8701 catatcttga agcagttggg catagattac cggccttaat tttgcgggat atgccgtttg
     8761 aaatatagag aatagctttc cagattttaa gtgggaaata taattctatt gacatttttt
     8821 ctgcttcgcg taagaatcgt tttcctgttt tcatttttaa ttttcgagga aattatgaac
     8881 acacgcatca actttaccgc aactgtgggt aagcgcgtga gccaatgcta tgttaccagt
     8941 gtaatcagca caatcggcgt taccacttcc gatgcaattt gttcgggagg cggaacgaac
     9001 aaagccaaaa gtcaatgcaa tgctttgctt aaagcggcag gccgctgcta atcctttatt
     9061 cggaaaaggt cgtctgaaaa tattttcaga cgacctttta tttattgagc aaactcccca
     9121 aactgccgac aatccgaccg ataaactact acaatttcgc gttgattttt tattatttcc
     9181 acgtaaagca aacaaatcac gtaagaacat taaaagtgta aagaaattgc ggacaaactc
     9241 cttaatatcc tgtaatcata tgtaatatgt gttagaatag cggagttgga ttttggggag
     9301 tcgctcgtct gtatcggcgg cgagtttcac aaagattaac atattgcttg acgtaataag
     9361 gcttctaaat tatattagcc gcgccccaaa cctgaggcgg aaacattccc tttatgaagc
     9421 agattatcat cctacaatcc gccgtattgt ccatatgcgc cgcagttgcc tttgccgtat
     9481 ggggttttgc cggattcctt tcggcggtcg ggggcggttt gtcctacctg cttcccacgt
     9541 ttgttgcagt tttacttcta aaacttttca ggggaaaccc cttcctgcaa agcaggatgt
     9601 tcgtcttcgg agagatttta aaagtagtgc tgtcgctgtt gtccatgctt gccgtatttg
     9661 caatatggca tcaatcgctg gtgttcgtcc cgtttttgat ggggctgctc ggtgtcagcc
     9721 atctggtttt tttagtattg ttgagagtga aagattatgg caggtgaaac cattaccgct
     9781 gccgactaca tcaagcacca cttgcagagc ttgaccagtt tgtcggatgt tactcagggt
     9841 cagggactga aaaacattgc tgatttttcg tttattaacc ttgatgccgt tttttttgcc
     9901 gtcctgttgg gcgtaatcgg cagcttcctg ttgtggcgcg gtgcaaaaaa agcaacggca
     9961 ggcgttcccg gacgtttcca ggccgccgtg gaaatcttgt tcgagtttgt ggacgatatg
    10021 tgtaagagca tcattcacag cgaaaagtct agaaaagccg tcgcgccgtt gggtctgacg
    10081 ctgtttgttt ggattttcct gatgaacgcg atggatatgc tgccggtcga tttgctgccg
    10141 atggtatggc agggcattac cggcaaccat cacgccctgc tgcgcatcgt accgactgcc
    10201 gatttgaaca ccgctttggc actcgctgtc ggcgtgttgc tgatttgtat ctattacaac
    10261 atcaaaatca aaggattggg cggctggttt cacgagttgt tcagtgcgcc gttcggtgca
    10321 aaactcgcac ctgcgaactt tctgttgaac ttggtcgagt ttctttctaa aaccgtatcc
    10381 cacggtatgc ggttgttcgg caatatgtat gcgggcgagc tggtattcct gctgatagcc
    10441 ttgcttggcg gtgcttgggc ggcttccggc agcgttgaag tcatggatcc gattctgttt
    10501 gtattccaca ttattgccgg tttggcatgg gcgattttcc atattttggt gattaccctg
    10561 caggcattta ttttcatggc gttggcgttc gtctatatcg ggcaggcgca cgatgcccac
    10621 tgattttccc aagtagtttg tttttgtagt ttaacctttg tttctttaag gagtttaaaa
    10681 tgggtttgat tgctatcgca tgtggtttga tcgttgcatt gggtgcattg ggtgcatcta
    10741 tcggtatcgc aatggtcggt tctaaatatt tggagtcttc tgctcgccaa cctgaactga
    10801 ttggtccgct gcaaaccaaa ctgttcctga ttgccggtct gattgatgcc gcattcttga
    10861 tcggtgtcgc cattgcacta ctgttcgcct tcgtcaaccc gtttgcaggt gcataatcag
    10921 gacggattcg tccgttcgta caggtgcagc ctgtttgatt aaccccaata cgaaggttaa
    10981 gtaacgtgaa tatcaatgca acattattcg ctcaaatcat cgtctttttc ggtttggtat
    11041 ggtttaccat gaaatttgtg tggccgccga ttgcaaaagc tttggatgag cgtgccgcaa
    11101 aaatcgccga gggcttggct gccgccgagc gtggtaaaag cgatttcgag caggctgaaa
    11161 aaaaggttgc agaacttttg gcagaagggc gtaatcaggt ttccgaaatg gttgccaacg
    11221 ccgaaaaacg tgccgccaaa attgtcgaag aagccaaaga acaggcttct tccgaggcgg
    11281 cgcgcattgc agctcaggca aaggccgatg tggagcagga attgttccgc gcacgcgaat
    11341 ccctgcgcga tcaggttgcc gtgttggctg tcaaaggtgc cgaatctatt ttgcgcagcg
    11401 aagtcgatgc ttccaaacac gcaaaactgc tcgataccct gaaacaggag ttgtaatctt
    11461 atggcagagt ttgcaacgat cgccagacct tatgcaaagg cattgttcgg tctggctcag
    11521 gaaaaaaacc aaattgagtc ttggttgggc ggactggaaa aacttgcggc ggttgttcag
    11581 gaagggaagg tggctttatt gattgaccgt cctgaaacga atgcttcaga aaaagcagat
    11641 atcctcatcg atttggtcgg tttgaaagac aaggagttaa aaaactttgt tatcgtcttg
    11701 gccgggcaga aacgtttgtc gatattgccg gaagtgtatg ctcaatatca agacttgacc
    11761 ttatcattca accatatcaa atctgccgtc atttacagtg cctatccgtt gaccgacaaa
    11821 caggtcggcg agttggcgca aatgctgaat aagcgtttcg acagcgagct gaaaatctct
    11881 gtcgaaatcg aaccggagct gattggcggc ataaaagttg aagtgggtga tcaggttttg
    11941 gatttgtctg tacaaggcaa actgagtgct ttgtacacga ctatgacgaa ttaggagagt
    12001 tttcatgcag cttaatcctg ctgaaattag cgatctgatt aaagccaaga tcgaaaatct
    12061 gtctgtaaat gccgaagtga gtacccgtgg tacggtgatt tcggtaactg acggtatcgt
    12121 tcgcatccat ggtttgtcag atgcaatgca aggtgagatg ctcgaattcc cgggtaacac
    12181 tttaggcttg gcgatgaacc tggagcgcga ctccgtcggt gccgtagtgt tgggcgagta
    12241 cgaacatatt aaagaaggcg acacggttac ctgtaccggc cgtattttag aagtgccggt
    12301 cggacgcgaa ttggtcggac gcgtcgtcga tgcattgggt cggcctatcg acggcaaagg
    12361 cccgattaat acaactttga ccgcccctgt cgaaaaaatc gcaccgggcg tgattgcacg
    12421 caaatcggtt gatcagccga tgcaaaccgg tctgaaggcg attgactcta tgattcctgt
    12481 cggtcgcggt cagcgtgagt tgattattgg agaccgtcag acaggtaaaa cggccgtagc
    12541 attggatgcc atcgtcaacc aaaaaggtac gggtgttatc tgtatctatg tcgctatcgg
    12601 tcaaaaagca tcttctattg ccaacgtggt ccgcaaattg gaagagcatg gcgcgatgga
    12661 acacacgatt gtggttgctg caactgcatc tgaagcggcg gcattgcaat atatcgcacc
    12721 ttattcaggt tgtacgatgg gtgaattctt ccgcgatcgt ggcgaagatg ccttgattgt
    12781 ttatgacgat ttgtccaaac aggctgtggc ttaccgtcaa atttccctgc ttttgcgccg
    12841 tccgcccggc cgcgaagcct atcccggcga tgtgttctac ctgcactccc gtctgttgga
    12901 acgtgcggca cgtgtcaatg aacacgaagt ggagaaattg accaacggcg aagtaaaagg
    12961 caaaaccggt tctctgaccg cgttgccgat tatcgaaacc caagcgggcg acgtatctgc
    13021 tttcgttccg actaacgtca tttcgattac cgacggtcag attttcttgg aaaccgacct
    13081 cttcaacgcc ggtatccgtc ctgcaatcaa tgccggtatt tccgtatccc gcgtaggcgg
    13141 tgctgcacaa accaaagtga ttaaaaagct gggtggcggt atccgtttgg cgttggcaca
    13201 atatcgtgaa ttggcggcgt tctcgcaatt tgcatccgat ttggatgaag ctacgcgcaa
    13261 acagttggag catggcgaag tcgtaaccga actgatgaaa cagaaacagt tcagtacgtt
    13321 gaatacggct gaaatggctt tgaccctttg ggcaatcaac aacggttcgt attctgatgt
    13381 tccggttgcc aaagctctag cttttgaatc tgaatttttg agctttgtcc gtacccaaca
    13441 tccggaagtt ttggaagccg tcaatgcttc aggtgcaatg tccgacgaga gcgagaaaac
    13501 gcttgaagca gccatgaaat ccttcaaatc ttcttacgcc tatcaggcat aaggcttgaa
    13561 gtgaaaggag tctgaaatgg cagtaggaaa agagattctc accaaaatcc gcagtgttca
    13621 gaatacccaa aagatcacta aagcgatgca aatggtgtca acctctaaaa tgcggaagac
    13681 tcaggaacgg atgcgtttgg cgcgtccgta tgccgaaaaa gtgcgtatgg tgatgagcca
    13741 tcttgcgcaa accaataccg atcatggtat tccgttactg gaatctcatc gggaaatcag
    13801 acgtgtcggt tttattttga ttacgtctga taagggtttg tgtggcggtt tgaacgccaa
    13861 cgtgctgaaa aagtttttgg cacaagttca agagtatcgg aatcaaggta ttgaagagga
    13921 aatcgtatgc cttggcagta aaggtctgat ggcgtgtcag agcattggtc tgaatgtggt
    13981 tgccagtgcc gtaaatttgg gcgatacccc aaaaatggaa atgctgctcg gacctttgac
    14041 agaacttttc caacggtatg agaaacatga aattgacaga atccatctgg tgtattcggg
    14101 ttttgtcaat accatgcgtc aagaaccgag gatggaagta ttgctgccta tcggtgagaa
    14161 cgtgattggc gattcagctc ccaaatcacc gttcagctgg gaataccgct acgaaccgac
    14221 tgcacttgca gtgttggaat atctggttcg ccgctattta gagtctgtgg tttatcaggc
    14281 gttgagcgac aatatggcat ccgaacaggc agcccgcatg gttgccatga aagctgcaac
    14341 agacaatgca ggcaatgcca tcaaagagtt gcgtctggta tataacaaat cgcgtcaagc
    14401 tgcgattacc acggaattgt cagaaattgt agcaggtgcg gcggccgtct gatgccgtct
    14461 gaatcctgta cggaaattag gatacgataa tgagccaagg caaaatcgta caaattatcg
    14521 gtgcggttgt tgacgtggaa tttccacgcg acatgattcc gcgcgtttac gacgctttga
    14581 aattagacga aaacggtctg actttggaag tccaacagct tttgggtgat ggcgtagtcc
    14641 gtaccatcgc tatgggcagc tcggacggtt tgaaacgcgg catgactgtg agcaatactg
    14701 gttcgcccat tactgtgccg gtaggtaaag gtacgttggg acgcattgtc gatgtattgg
    14761 gaactcctgt tgacgaggca ggtccaattg ataccgacaa gagtcgtgcc atccaccaag
    14821 ccgctcctaa gtttgacgaa ctgtcttcca caaccgaatt gctcgaaacg ggcattaaag
    14881 tgattgactt gctgtgtccg tttgccaaag gcggtaaagt aggtctgttc ggcggtgccg
    14941 gtgtgggtaa aaccgtgaac atgatggaat tgatcaacaa catcgccaaa gcgcacagcg
    15001 gcttgtccgt gttctcaggc gtgggtgagc gtacccgcga aggtaacgac ttctaccacg
    15061 agatgaaaga ttccaacgta ttggataaag tagccatggt gtatggccaa atgaacgaac
    15121 ctccaggcaa ccgtctgcgc gttgctttga ccggtttgac tatggctgaa tacttccgtg
    15181 acgaaaaaga cgaaaacggt aaaggtcgtg acgtattgtt cttcgttgac aacatctacc
    15241 gttacactct ggccggtacc gaagtatccg cactgttggg ccgtatgcct tctgcagtgg
    15301 gttaccaacc gacattggct gaagaaatgg gtcgtttgca agagcgtatt acctctaccc
    15361 aaaccggttc cattacttcc atccaagccg tatatgtacc tgcggatgac ttgactgacc
    15421 cgtctccggc gacaactttc gcccacttgg acgcgaccgt cgtattgagc cgtgatattg
    15481 cctctttggg tatttacccg gcagttgacc cgcttgactc tacttcgcgc caattggatc
    15541 cgatggtatt gggtcaagag cactacgacg tagcgcgcgg tgtacagtct actctgcaaa
    15601 aatacaaaga attgcgcgac atcatcgcca ttctgggtat ggacgaattg tctgacgaag
    15661 acaaactggc tgtaatgcgt gcccgtaaaa tccaacgctt cctgtctcaa ccgttccacg
    15721 ttgccgaagt gtttacaggt tctccaggca aatatgtcgc cctgcgcgat accattgccg
    15781 gcttcaaagc catcttgaac ggcgaatacg atcatctgcc cgaacaggca ttctatatgg
    15841 tcggcagcat cgaagaagcg gttgagaaag cgaaaacctt aaactgagga ggtcggcatg
    15901 agcatcatgc aagttgaggt ggtaagtggc gagcagaaaa tctattcagg cgaggcaaca
    15961 tttatcgttg ttccgactgt acagggcgaa ctcggtattt atccgcgaca cgagccgatt
    16021 atgagtttgg tgcggccggg ggctttgcgt ttgaccgttc cgggcgagga taaagaggtt
    16081 ttggttgctg tttccggcgg tattttggaa gtacagcctg ataaagtaac tgtcttggcg
    16141 gatgttgccg tccgcagtgc ggaaatggat cgggcacgtg cggaagaggc gaaaaaagcc
    16201 gcagaagcgg gcatttccca agctaaagac gataaggctt tggcggaagc acataaagca
    16261 ttggctgctg cgattgcgca gctcaaaact ttggactata tccgttcgca caagaaataa
    16321 acttgtttgg tttgaaaaag cacgaccgtt tggccgtgct ttttattttc cggatttaaa
    16381 ttaaaaaatt atccatcccc tggtttcaaa tcaggctgtc ttcgagataa atctattcaa
    16441 gcggctatgg aaacagtaaa ataaaacctt tattttatcg aacaataagc catgcttacc
    16501 ttccaacaaa tcatcttcaa actgcaaacc ttctgggcag acaaaggctg caccgtcatc
    16561 caacccttcg acatggaagt cggtgccggc acatcccatc ccgccacctg cctgcgcgcg
    16621 ctcggccccg agccttggtt tgccgcctac gtccaaccca gccgccgccc caaagacggc
    16681 cgctacggcg acaaccccaa ccgcctgcaa cactattacc aattccaagt cgccctcaaa
    16741 cccgcccccg ccaatatcca agacctctat ctcgactccc tgcgcgaatt gggcatcgat
    16801 cccaaagtcc acgacatccg ctttgtcgaa gacgactggg aaaaccccac cctcggtgcg
    16861 tggggtttgg gctgggaagt ctggctcaac ggcatggaag taacccagtt tacctatttc
    16921 caacaagtcg gcggtatcga ttgcacgccc gtactcggcg aaattaccta cggcatcgaa
    16981 cgcttggcga tgtacctgca aggcgtggaa aacgtctatg acctcgtttg ggcaaaaacg
    17041 ccggacggca ataccgtaag ctacggcgac gtgtaccatc aaaacgaagt cgagcaatct
    17101 acctacaact tcgaatacag cgatgccgac tggctgctgc gccagttcaa cgactacgaa
    17161 gcgcaagcca aacgcctgtt cgccgaagaa aacgccgacc ttgccttacc tgcctacgag
    17221 ctggtcctca aagcggggca tacgttcaac cttttagacg cacgcggcgc gatttccgta
    17281 accgagcggg caacttatat tggacgtatt cgtgcattga gccgcgccgt ggcgcaaaaa
    17341 tatatcgaaa gccgtgagaa actgggcttc ccgttgatta aaaaataacg atacgtcgtc
    17401 tgaagcaggt tttcaggcga cctttattgc gtatccatta atacaggccg tacggcatat
    17461 gcaggcttta taagcatggc ctacggacaa acagaaagtc tcacatgaca acccaaaccc
    17521 ttttaatcga actccttacc gaagaactcc cgccaaaagc cctgaataat ctgggcaacc
    17581 attttgccgc ttccgttgcc gaaggcttgg aaaaagcgca actggttgac ggcgcggccg
    17641 aatttacggc ttatgcctcg ccgcgccgtt tggcggttca agtcaaaaac gtcaaagctg
    17701 ttcaggccga tcagaaaatc gtgaaaaaag gccctgccgt ggcgaatgcc gtaaaagacg
    17761 gtacgccgac caaggctttg gaaggttttg cgcgcggggc gggggcgaaa atcgaagacc
    17821 tgaccatcat ccacgacggc aggcaggacg tgtacgccta cgaatacgtc caaaccggca
    17881 gaccgttggg cggacttttg gaagacatta tcaatcaagc tgttaagaag ctgccgattc
    17941 cgaaagtaat gcgttggggc agcagcacgt ttaccttcgt gcgccctgtt cacgggctga
    18001 ttgtgctgca cggcggcgat gtcgtgaacg tcagcgtttt gggcctgcaa agcggcaatc
    18061 aaaccttggg acaccgcttt ctttccgacg gcgaaatgat cattgaaaac gccgacagct
    18121 acgccgcaca aatgcgcggg caaggcaaag tcgtcgcttc gtttgccggg cgcaaagccg
    18181 cgattcagac ggcattggaa gggcaggcac gccgtctgaa cgcgaccgtt gccgccgatg
    18241 aagccctgtt ggacgaagtg accgcgctgg tcgaatggcc tgttgttttg gaagccggtt
    18301 ttgaggaaca cttcctcgcc gtgcctcaag aatgcttgat tttgacgatg cagcaaaacc
    18361 aaaaatactt cccgctgctc gaccaaaacg gcaagctgat gaaccgcttc ctgctggttt
    18421 ccaacctgca aaccgaagac ccgtcgcaca tcatccgagg caacgaacgc gtattgcgcg
    18481 cgcgcctgtc tgatgccgag ttcttctaca aacaagacca aaaagcgacc ttggaaagcc
    18541 gcctgcccaa gctggcgaac gtggtgtacc acaacaaaat cggttcgcag gccgaacgca
    18601 tcgaacgcct gcaaagcatc gccgcccaca tcgccaaagc attgggcgcg gatgccgcgg
    18661 cagccgagcg cgccgcgcgt cttgccaaag ccgacttggt aactgaaatg gtcggcgagt
    18721 tccccgaact gcaaggcacg atgggcaaat actacgcccg cttggacggc gaaaccgaag
    18781 aaatcgccga agctattgag caacactacc aaccgcgttt tgccggcgac aagctgcccg
    18841 aaagcaaaat tgccgccgcc gtggcacttg ccgacaaact ggaaaccctg gtcggcattt
    18901 ggggcatcgg tttgattcct accggcgaca aagaccccta cgccctgcgc cgcgccgcct
    18961 tgggtatttt gcggatgctg atgcagtacg gtttggacgt gaacgaactg attcagacgg
    19021 cattcgacag cttcccgcaa ggtttgctca acgagaaaac gccgtctgaa accgccgact
    19081 ttatgcaggc gcgccttgcc gtgttgctgc aaaacgatta tccgcaagac atcgttgccg
    19141 ccgtactcgc caaacagccg cgccgtttgg acgatttgac cgccaaactg caggccgtcg
    19201 ccgtattcaa acaactgccc gaagccgccg cgctcgccgc cgccaacaaa cgcgtgcaaa
    19261 acctgctgaa aaaagccgat gccgcgttgg gcgcagttaa cgaaagcttg ctgcaacagg
    19321 acgaagaaaa agccctgtac gctgccgcgc aaggtttgca gccgaaaatc gccgccgccg
    19381 tcgccgaagg caatttccga accgccttgt ccgaactggc ttccgtcaag ccgcaggttg
    19441 atgccttctt cgacggcgtg atggtgatgg cggaagatgc cgccgtaaaa caaaaccgcc
    19501 tgaacctgct gaaccgcttg gcagagcaga tgaacgcggt ggccgacatc gcgcttttgg
    19561 gcgagtaacc gttgtacagt ccaaatgccg tctgaagcct tcaggcggca tcaaattatc
    19621 gggagagtaa attgcagcct ttagtcagcg tattgatttg cgcctacaac gtagaaaaat
    19681 attttgccca atcattagcc gccgtcgtga a
//
LOCUS       DAXDXW010000039        19296 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXDXW010000039 DAXDXW010000000
VERSION     DAXDXW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19296
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.040"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(78..1148)
                     /gene="lptG"
                     /locus_tag="ACFB64_001177"
     CDS             complement(78..1148)
                     /gene="lptG"
                     /locus_tag="ACFB64_001177"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC1209541.1"
                     /translation="MNLISRYIIRQMAVMAVCALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLASLSQLAAGSELAVIKASGMSTKKLLLI
                     LSQFGFIFAIAAVALGEWVAPTLSQKAENIKAAAINGKISTGHTGLWLKEKNSIINVR
                     GMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSIMGTDKIETSA
                     AAEETWPIAVRRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTQIYAIAWWRKLVYP
                     VAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGTPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            complement(1145..2260)
                     /gene="lptF"
                     /locus_tag="ACFB64_001178"
     CDS             complement(1145..2260)
                     /gene="lptF"
                     /locus_tag="ACFB64_001178"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC1209542.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAILIAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNNLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTISTAQLIGSSNPQHQAELMWRISLTVN
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            2249..2431
                     /locus_tag="ACFB64_001179"
     CDS             2249..2431
                     /locus_tag="ACFB64_001179"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209543.1"
                     /translation="MINHKIAVRGAVSGSAQADCPRKVSDGASKNGVQSCQPSSDGLL
                     SERPSENYRQPSQSGE"
     gene            2434..3840
                     /locus_tag="ACFB64_001180"
     CDS             2434..3840
                     /locus_tag="ACFB64_001180"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC1209544.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCADKAPEHNTAAHALFSALEEGQ
                     NFSDTKIPTDNGLQAVAVVRLEKTDRAALNKAAAEAAKWAQNQETVNVDVHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFSQAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAIVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLMNYLRNL"
     gene            3903..4343
                     /locus_tag="ACFB64_001181"
     CDS             3903..4343
                     /locus_tag="ACFB64_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC1209545.1"
                     /translation="MPKVTFYTHVDQIPLFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSDTSVLLACGGNLPRIPEGMAVLNLSDGFWNTA
                     SVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            4603..5421
                     /locus_tag="ACFB64_001182"
     CDS             4603..5421
                     /locus_tag="ACFB64_001182"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC1209546.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASAAQGDT
                     SSIGGTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTDAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPATFPLSQVIPGWTEGVRLLKEGGEA
                     TFYIPSNLAYREQGAGEKIGPNATLVFDVKLVKIGAPENAPAKQPDQVDIKKVN"
     gene            5528..6154
                     /gene="purN"
                     /locus_tag="ACFB64_001183"
     CDS             5528..6154
                     /gene="purN"
                     /locus_tag="ACFB64_001183"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC1209547.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTESLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGQIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            6170..6841
                     /locus_tag="ACFB64_001184"
     CDS             6170..6841
                     /locus_tag="ACFB64_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC1209548.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAARLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPAYYKDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDVKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVTYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            6959..7381
                     /locus_tag="ACFB64_001185"
     CDS             6959..7381
                     /locus_tag="ACFB64_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC1209549.1"
                     /translation="MQVTSKWIDGMCFVGTAEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVIGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGAEK"
     gene            7902..8618
                     /locus_tag="ACFB64_001186"
     CDS             7902..8618
                     /locus_tag="ACFB64_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC1209550.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILAGLPDLETRLKPLYAMIRCEEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREKKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            8767..9669
                     /locus_tag="ACFB64_001187"
     CDS             8767..9669
                     /locus_tag="ACFB64_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFC1209551.1"
                     /translation="MSSLMTLFSVLVPMFAGFFIRVPKPYLPASDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLGDMALTVLWLFVCTVGANLLALAVLGKLSPWRIGGKGKGVSVG
                     VSGSVRQLGCVLIGFVSGKLMCDIWMPSENAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     LNRRGIRLSVWFILSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            9712..10485
                     /locus_tag="ACFB64_001188"
     CDS             9712..10485
                     /locus_tag="ACFB64_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFC1209552.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRCDIREL
                     EEGGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAAASELVKRRSSLRIITNNIHVASVVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDYDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGGITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            10597..12285
                     /locus_tag="ACFB64_001189"
     CDS             10597..12285
                     /locus_tag="ACFB64_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC1209553.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     DYFDRLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMAAGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGVRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFQAGKTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCGEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPGAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRQDHGKDTPVFNRTVTLKDSWQPK"
     gene            12377..13333
                     /gene="gshB"
                     /locus_tag="ACFB64_001190"
     CDS             12377..13333
                     /gene="gshB"
                     /locus_tag="ACFB64_001190"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC1209554.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     NGGLVTAQASAFEFSGAKNDDDHEWFKAADKVQTALKEFDAVIMRTDPPFDMQYLYST
                     QLLTLAEQQGAKVFNSGRAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IVKPLDGMGGMGIFRLTEKDPNIGSILETLMRFDSRTIMAQRYIPEIVHGDKRILIIG
                     GKVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRSGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKSFDVAAMFADAVAAWSVR"
     gene            13492..13875
                     /locus_tag="ACFB64_001191"
     CDS             13492..13875
                     /locus_tag="ACFB64_001191"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFC1209555.1"
                     /translation="MEPSSYAAEKKGKSGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNALLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLVAMLMLAAVWLSALFG"
     gene            14007..14480
                     /locus_tag="ACFB64_001192"
     CDS             14007..14480
                     /locus_tag="ACFB64_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC1209556.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKMGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMPAMNHGHHHGEAHQH"
     gene            14605..15708
                     /gene="mnmA"
                     /locus_tag="ACFB64_001193"
     CDS             14605..15708
                     /gene="mnmA"
                     /locus_tag="ACFB64_001193"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC1209557.1"
                     /translation="MNTTSNTSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWEN
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTIGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELCYLDDETAELVFDEPQW
                     AVTPGQSAVLYDVDICLGGGIIQTTDKPVIITR"
     gene            15779..17449
                     /locus_tag="ACFB64_001194"
     CDS             15779..17449
                     /locus_tag="ACFB64_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC1209558.1"
                     /translation="MEKIWLESYEKGVSAEIDITQYNSVSDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVARGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLIANPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVPSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDACGFLETGDIAVMDEKGRLK
                     LVDRKKDLVVVSGFNVYPNEIEEFIAHHEKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LIKEELTAFCRTGLTAYKVPKDIEFRDELPKSNVGKILRRELRQSAGK"
     gene            17561..19195
                     /locus_tag="ACFB64_001195"
     CDS             17561..19195
                     /locus_tag="ACFB64_001195"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC1209559.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRKMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAVKNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NKGDVSVLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAVKIYGSGHIRERHRHRYEVNNNYVSALEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         4733 a         5173 c         5235 g         4155 t
ORIGIN      
        1 agggaacggc tatatgtctg cgggaatgtg tcaatgaaaa caaatgccgt ctgaagagag
       61 gttcggacgg catttgttca acgtttttcc tgtttgcgta tcagccaaac agcgagcaag
      121 gcgaaggcta tggtaggcag tgcgccggcg aggaagggtg gggtgccgta gagttggctg
      181 gtaaacccga agagcctgcc ggcaaggtgg aacagcaatc cgagacagat gccgccgaag
      241 agttttaagc ccatattgcc gtggcgcgtg gtttgcggcg taaaggcgaa ggcaacgagc
      301 gccatgaccc atgcggcgac ggggtaaacg agtttacgcc accatgcgat ggcgtagatt
      361 tgggtgtttt ggctgttgtt ttggaggtgg cggatgtagg tggtcagctc gccgacggac
      421 atttggtcgg gcttgacgag caatacgtcc atcaggttgc gtctgacggc aatcggccaa
      481 gtttcttcgg cggcggcgga tgtttcgatt ttgtctgtac ccatgatgct gcggcggatg
      541 tttttcaact gccagctgcc gtcgctgttc aaaacggcgg aatcggcttc cactgcctct
      601 gccaattcgt ttttatcgtt gcgcgcccaa attttgatgc ccaaaagcgt atggtcgggc
      661 aacattccgc gcacattgat aatgctgttt ttttctttca gccaaaggcc ggtatggccg
      721 gtgctgattt tgccgttaat ggcggcggct ttgatgtttt cggctttttg gctcagcgtg
      781 ggcgcaaccc attcgccgag cgcgacggcg gcaatagcaa aaataaaacc gaactgagac
      841 agaatcaaca gcagcttttt ggtgctcatg ccgctggctt tgatgacggc cagttcgctg
      901 ccggcggcaa gctggctgag agaggccagt ccgccgatga ggacggcgag gggcatgagt
      961 tcgtaggcgc gggcgggcat tttgagggcg gtgtagccca gcatttccca tatgccgtaa
     1021 ctgcctttgc cgaggttgcc ggtttcgtac aggatttcaa aaaagctgta caaagcgagg
     1081 aaggcaagga gcgcgcaaac cgccataacc gccatttggc ggatgatgta acgtgaaatc
     1141 aggttcattt tccgcctttc aatgtcagac ttttgccaac cgcctgccag aagggctggc
     1201 tgggcatact gcggacgcgc agaagtacga ttgcgatgac gaacatgatg atgtgcatag
     1261 gcagcagtcc gagccaaaaa tggattttgc cgtcttccac ggcttcaaaa agcagggtca
     1321 gcccgttttg gtaaattaaa aacaaaccga tggcaatcaa gatattgtag gtatgtccgc
     1381 tgcgcgggtt gaaataggaa agcggcacgg cgagtaggca gagcaggagg acgttgacgg
     1441 tcagcgagat gcgccacatc aattctgcct gatgttgcgg attgctgctg ccaatcagtt
     1501 gggcggtcga aatggtgcgg cggtgggaaa cggggtcgat aagtttgggc gtggtgctga
     1561 taatcaggtt gagtttttgg aaggaaacct gattgtagtc ggcgcgtccg ggcgtgccgc
     1621 tgtaacggta gccgtggcgc aattcgagcg tgcgtttgtt gtcgttcagc gagaagttac
     1681 cttctttggc gaagatgatg ttgtcgccgc cgtttttgtc ctgttcgcgc aggaacaggt
     1741 ttttcatgat gccggattcg gtgtcaaagg tttcgacgaa ataaaccctg ccgttgcgct
     1801 tgcccaagtt attgaactcg ccggcttcca ccaaagacaa ttcctgcttc tgcttcaaaa
     1861 tttcggcata ttcgcggctg cgcagttccg cccacggtat cacccaaagc tgcatgacgg
     1921 caatcaggat ggcaaacggc acggcaaact gcatgacggg gcgtatccac tgtttcaacg
     1981 ccaatccgca ggatagccag accgacattt cgctgtcgcg ccagtagcgg gtcaatacgg
     2041 tcagcgtgct gatgaatgcg gtcaacacca gcaaaagcgg ggtcataccg atgacccaga
     2101 agccgactaa ggccaacacg gcatcgatgg cgacacgccc gtcagctgcg cggccaagca
     2161 ggttgatcgc ctgcgtggac accaacaccg ccaagaggac gacgaaaatg ccgacggcgg
     2221 taaaagagag ttctttgatg aggtttcttt gataaatcat aaaatcgcgg tcaggggggc
     2281 ggtaagcggt tcggcgcagg cggattgtcc gcgcaaggtt tcagacggtg cttcaaaaaa
     2341 tggggtacaa tcgtgccagc cgtcttcaga cggtcttttg agcgaaaggc cgtctgaaaa
     2401 ctaccgacaa ccgtctcaat caggagaata aacgtggaat ttagcacaaa aaccgaaatc
     2461 ttgcaggaac agcaggcagg cgcgcagtta tttgtctgtg ccgacaaagc acccgagcac
     2521 aacaccgccg cacatgcgct cttctctgct ttggaagagg gtcagaattt ttccgacacc
     2581 aaaatcccga cggacaacgg tttgcaggca gtcgccgtcg tccgcctcga aaaaaccgac
     2641 cgcgccgcgc tgaacaaagc cgccgccgaa gccgccaaat gggcgcaaaa tcaagaaacg
     2701 gtcaatgtgg acgttcacgc ctttgatgag gcgcaagcag cagccgttgc cgaagctttt
     2761 gccatcgcgt tcggcaacgc tgcatatcgt ttcgaccgct acaaaaaaga agccaagccc
     2821 gccaaatttt cgcaagccgt gttccacagc gcacacgaag ccgccgtcaa agaagccctg
     2881 cgcgttgccg aagcacaagt ttacggacaa agcctctgcc gcgacttagg caatgccgca
     2941 ccgaacgaat gcacgcctga gttcctcgcg cgtaccgcca aagccgaagc cgagaaactg
     3001 ggcgcgcacg ccaaaatcat cgaaaaagac tacatcaaag aaaacatggg ttcgttttgg
     3061 tcggttgcca aaggcagcgt cgaagaccca tatttggttg aactgagcta tttcggtgcg
     3121 gccgacaaag aagccgcgcc tgtggtattg gtcggcaaag gcattacctt cgacaccggc
     3181 ggcatttccc tcaaacccgg cctgaacatg gacgaaatga agtttgacat gtgcggcgcg
     3241 gcaaccgtca tcagcacctt ctgcgccgcc gtcaaactgc aactgccgat caatctgatt
     3301 gccatcgtcg ccacttgtga aaacatgcct tccggcgcgg caaacaaacc gggcgatgtt
     3361 gtgaaaagta tgaaagggct gacgattgaa gtgttgaaca ccgatgccga aggccgtctg
     3421 attttgtgcg acgcgctcac ttacgccgag cagttcaaac ccaaagccgt catcgatgtc
     3481 gccaccctga ccggcgcgtg catcgtcgcc ttggggcacg atgtcagcgg cgtgatgggc
     3541 aataatcagg atttgatcga cagcctgctt gccgcttcct acaacgtgga cgacaaagcg
     3601 tggcaactgc cgctctttga aacctacaaa gaccagctca aatccaactt tgccgacatc
     3661 cccaatatcg gcacgcccgg cgcgggcacg attaccgccg caacattcct gtcctatttc
     3721 accgaaggct acccgtgggc gcaccttgac atcgcgggta cggcgtggaa atccggagcg
     3781 gaaaaaggtg cgaccggccg ccccgtaccg ctgctgatga actatctgcg gaatctttaa
     3841 ttttgccaca atgccgtctg aagcttttca gacggcatca tcctgtcaaa tgctcaaaca
     3901 atatgccgaa agttaccttt tatacccatg ttgaccaaat cccccttttt acctgtcggt
     3961 tgattgcgcg cgctatccga gacggcggca ggatactggt gtggtccgac tcgttcgggc
     4021 ggcttcagga attggacaaa atgctttggc aatacgaggc cgagagtttt attccgcacg
     4081 aaatttggga aacggaagaa gccatgccgt ctgacacatc cgtcctgctt gcctgcggcg
     4141 gcaatctgcc ccgaattccc gaaggcatgg ccgttttgaa cctgtccgac ggtttttgga
     4201 acaccgcttc ggtcttgccc gcgcgcgttt tggaaatcgt cggcaacagc ctggaagatc
     4261 tcgccgacgc gcgcgaacgc tttaccgcct accgccgaag cggttttgcc atcgaacatc
     4321 acggcatgga gggcaaggca tgaaatgccg tctgaaatgc cgcatttaca aaatttaaac
     4381 aggcttgatt ctatttgatt aaaatttcct tatcggttga accccgccac ttggacatct
     4441 gtccttcggg gcggtagaat caaaccttat tttggaaggt tcaatccctt ccaaaacagg
     4501 gcaacacaca gattgacgct ttatgtgcca tcctgtgtgt tgaaacattc aaactcggct
     4561 ataatcccgt ttttccgact ttatcgacag cgaagatcca tcatgaacac cattttcaaa
     4621 atcagcgcac tgaccctttc cgccgctttg gcgctttccg cctgcggcaa aaaagaagcc
     4681 gcccccgcat ctgcatccga acctgccgcc gcttctgccg cgcagggcga cacctcttca
     4741 atcggcggca cgatgcagca ggcaagctat gcgatgggcg tggacatcgg acgctccctg
     4801 aaacaaatga aggaacaggg cgcggaaatc gatttgaaag tctttaccga tgccatgcag
     4861 gcagtgtatg acggcaaaga aatcaaaatg accgaagagc aggcccagga agtgatgatg
     4921 aaattcctgc aggagcagca ggctaaagcc gtagaaaaac acaaggcgga tgcgaaggcc
     4981 aacaaagaaa aaggcgaagc cttcctgaag gaaaatgccg ccaaagacgg cgtgaagacc
     5041 actgcttccg gtctgcagta caaaatcacc aaacagggtg aaggcaaaca gccgacaaaa
     5101 gacgacatcg ttaccgtgga atacgaaggc cgcctgattg acggtaccgt attcgacagc
     5161 agcaaagcca acggcggccc ggccaccttc cctttgagcc aagtgattcc gggttggacc
     5221 gaaggcgtac ggcttctgaa agaaggcggc gaagccacgt tctacatccc gtccaacctt
     5281 gcctaccgcg aacagggtgc gggcgaaaaa atcggtccga acgccacttt ggtatttgac
     5341 gtgaaactgg tcaaaatcgg cgcacccgaa aacgcgcccg ccaagcagcc ggatcaagtc
     5401 gacatcaaaa aagtaaatta agtccgaatc catgcccgaa acaggttttc gggcattttt
     5461 acggcaaatg ccgtctgaag ccaccaaaca gcggttcaga cggcatccct ttcaaaagcg
     5521 caccattatg aaaaacatcg tcatcctgat ttccggacgc ggcagcaata tgcaggcaat
     5581 cgtcaatgcc gccattccca acgtccgcat tgccgccgtg ttgagcaaca gcgaaacggc
     5641 tgccggtttg caatgggcgg ccgaacgcgg catcccgacc gagagcctga atcataaaaa
     5701 ctttgaatca cgacttgcct tcgatacggc catgatggaa aaaatcgacg catatcaacc
     5761 cgatttggtg gttttggcag gttttatgcg gattctgacc cctgagttct gcgcccatta
     5821 cgaaggcagg ctgatgaaca tccatccgtc catcctgccc tcgttcaccg ggcttcatac
     5881 gcacgaacgc gctttggaag caggttgccg cgttgccggc tgtacgattc atttcgttac
     5941 ggccgagctg gattgcggcc agattgtatc gcaagggatt gtgccgatac ttgacggcga
     6001 tacggcagac gatgttgccg cccgggtttt ggctgtcgaa cacaaactct atccgaaagc
     6061 cgttgccgat tttgccgccg gccgcctgat tatcgaggga aaccgtgtca gaaattcgga
     6121 aaacgccgat gccgcccgtt tcctgacggc gtaaaccggg cgggagcaaa tgatgaagac
     6181 ttttaaaaat atattttccg ccgccatttt gtccgccgcc ctgccgtgcg cgtatgcggc
     6241 aaggctaccc caatccgccg tgctgcacta ttccggcagc tacggcattc ccgccacgat
     6301 gacatttgaa cgcagcggca atgcttacaa aatcgtttcg acgattaaag tgccgctata
     6361 caatatccgt ttcgaatccg gcggtacggt tgtcggcaat accctgcacc ctgcctacta
     6421 taaagacata cgcaggggca aactgtatgc ggaagccaaa ttcgccgacg gcagcgtaac
     6481 ctacggcaaa gcgggcgaga gcaaaaccga gcaaagcccc aaggctatgg atttgttcac
     6541 gcttgcctgg cagttggcgg caaatgacgt gaaactcccc ccgggtctga aaatcaccaa
     6601 cggcaaaaaa ctttattccg tcggcggcct gaataaggcg ggtacgggaa aatacagcat
     6661 aggcggcgtg gaaaccgaag tcgtcaaata tcgggtgcgg cgcggcgacg atacggtaac
     6721 gtatttcttc gcaccgtccc tgaacaatat tccggcacaa atcggctata ccgacgacgg
     6781 caaaacctat acgctgaagc tcaaatcggt gcagatcaac ggacaggccg ccaaaccgta
     6841 aatcggtttt caggcggttt tttgccgttt tgtccatatt tctgtataag ccgcttgaaa
     6901 ttgtttacaa taacgccata tgcaaagcgg ttttaacgct attttcggga atgacaccat
     6961 gcaggttaca tcaaaatgga tagacggaat gtgttttgtc ggcacggcgg aaggcgggca
     7021 cagcgtcgtt atggagggat cggcggcaga aggcgcggcc aagcgcgggc ccagcccttt
     7081 ggaaatgctg ctgttggggg tggcgggctg ttcgagcatc gatgtggtga tgattgccga
     7141 aaaacagcgt cagaaagtga ctgactgccg tgcgacggtt acggcgaaac gggcggacga
     7201 tgcgccgcgc gtgtttaccg aaatccacat ccatttcaag gtaatcgggc atgatttgaa
     7261 agaatcggcc attgagcgcg ccgttcagat gtctgccgaa aaatactgtt cggcttcgat
     7321 tatgttgggc aaagcggcaa agattaccca cagttttgaa attgccgggg cggagaaata
     7381 ggcatcgggc ggcattatgg ggaaacggct gatgccgtct gaaggggaaa ggtgcggcag
     7441 gcaatccgga ggcgcataga aaagcagata tattcggact gcacctcccg aatatatctg
     7501 cctgctgttt cctctttatt cagcctttat aatacttgga cttgtcgggg tattgtgcag
     7561 gcttgattcc ggattgtcaa caattttcgg tcaaatttta aatgccgcgt tttaaaatga
     7621 tgcccgcctg cttctgcggg cgggcggaag gcgggaatcc cgttatttta agcaatatat
     7681 taatttattg aattaaaata aatttgtgca atatagtgga ttaacaaaaa ccagtacggc
     7741 attacctcgc cttgccgtac tatttgtact gtctgcggct tcgtcgcctt gtactgattt
     7801 aaatttaatc cactatactt taatttctcc tacacgattt gaaacacagc cgtcttaaaa
     7861 catcttaaaa aatttgaaag cgtaaaaata cggaaaacat catgaacttt gaaaacgacg
     7921 acattatcca tgcgccgacc acgtcttccc tgattctcga agagcggcac gattcggagc
     7981 tgttccgtgt ttacgccctg attttggacg gcattaccga tcaggtgctg ctgcccggca
     8041 aaaagctgac cgaatccgaa ctttgccgtc agatggtgtg ttcgcgcaac accgtccgcg
     8101 gcgcgctgtc gcttttggcg cacgacaaga ttgtcgattt gcaacccaac aggggcgcgt
     8161 tcgtccacgt tcccgatttg aaagaaatgc aggatgtgtt caatgcgcgc atcgaaatgg
     8221 agacgatgat tttgaacatc ctcgccggcc tgccggattt ggaaacgcgc ctcaagccgc
     8281 tttatgcgat gataaggtgc gaagaagagg cctccggcag gggcgaccgc gtcggctgga
     8341 accgcctgtc caatgccttc cacgtcgaac tggcgcgcct ggtgggcaac gatgtgctgt
     8401 tcgacattat gaacacgctg tgcgcgcggt cttccctgat tgtcgccgtg gcgggcgtgc
     8461 atcgcgagaa aaaacacgcc atcaatacgc atacgcattc cgaacaccgc gaaatcctcg
     8521 acctgctgct ggcgggcagg cgcaaccgcg tggtcaaaat cctgcgccgc catttgggca
     8581 actgtatgga gcgtttggaa aagactttgg aagattgaat gccggagcgg ataagccgcc
     8641 tgaaccttca ggcggttttt taatggcgaa cctgatgccg tctgaaatat ggatgcgggt
     8701 atctgcaatt ttcagacggc aatttttaag ccgcacatat catgcggcaa taaaggaggg
     8761 taggggatga gcagcctgat gacgttgttt tcggtattgg taccgatgtt tgccggattt
     8821 tttatccgtg ttcccaagcc ttacctgccc gcttcggaca aggtgctgtc ggttttggtg
     8881 tatgccgtgc tgctgctgat cggcgtatcg ttgtcgcgcg tggaggattt gggttcgcgg
     8941 ttgggcgata tggcgttgac ggttctgtgg ctgtttgttt gtacggtagg ggcgaacctg
     9001 cttgccttgg cagtgttggg aaagttgtcc ccgtggcgga tagggggaaa agggaagggc
     9061 gtttcggtcg gcgtgtcggg cagtgtgagg cagctcggat gcgtactgat cggttttgtg
     9121 tccggcaaat tgatgtgcga tatttggatg ccgtctgaaa acgcgggtat gtactgcctg
     9181 atgctgctgg tgttcctcat cggcgtacag ctcaaaagta gcggcgtatc gttgcggcag
     9241 gttttgctta accggcgggg catccggctg tcggtttggt ttatattgtc atctctttca
     9301 ggcgggctgc tgtttgccgc atcggcagat ggtgtgtcgt ggacgaaagg tttggcgatg
     9361 gcttccggct tcggttggta ttccctctcg ggtttggtaa tgaccgaggc ttacggggcg
     9421 gtatggggca gcatcatgct gctgaacgat ttggcacgag agctgtttgc actggcattt
     9481 attccgctgc tgatgaagcg ttttccggat gcggcggtgg gggtcggcgg cgcgaccagt
     9541 atggatttca cattgcccgt aattcagggt gcgggcggtt tggaagtcgt gccggtagcg
     9601 gtcagcttcg gcgtggtggt caatatcgcc gccccgtttc tgatggtggt gttttccacg
     9661 ctgggctgaa cgcggtaaaa tcggcatccc gatgcaagga agcagaaaac gatgaaaccg
     9721 aaaatccaaa ggcatggaga gattttaagc cttgtccgcc ggcatcagtt tatgtcggtg
     9781 gacgagcttg ccgccgcatt ggacgttacc ccgcagacga tacgttgcga catccgcgag
     9841 ttggaggagg gcggcagcct gaaacgccat cacggcggcg catcttcggg cggaaacttg
     9901 ccggagggtc tgcccgccga ccgccaaacc cggtgtcaaa acgaaaaaaa cgccattgcc
     9961 cggctgattg cggaacacat ccccgacggt tcgtcgctgt tcgtcagtat cggtacgacc
    10021 atggaagccg cggcatcaga gctggtgaag cggcgcagca gcctgcggat tattaccaac
    10081 aatatccacg tcgcctccgt cgtttcggca cgtacggatt acacggtcat catcacatcc
    10141 ggcgtcgtcc gccctttgga cggcggtatt accggcgtgg cgaccgtcga ctttatcaac
    10201 cagttcaaag tcgattatgc cgtgatgagc acgcacggcg tggaaagcga cggttcgctt
    10261 ttggattacg attacaagga agtcagcgtc atgcaggcga tgattgccaa cgcgcgcgtc
    10321 cgttttctcg gcgtggatca cagcaaattc cgcagcaacg cgctggtcag gctcggcggc
    10381 attacggcgt ttgacaaagt atttaccgac aggttgcctg ataccgcgat gcagaagatg
    10441 ctgaaagagg cgggggtgga atgcctgatt gccgatgccg tctgaacgct atgtcaaagc
    10501 gcgcaagtcg ggtacaataa acacatcatc aaaccgcttc agacggcata cggaacctcc
    10561 ccaatgccgt ctgaagccat ctgtttaaag aaaaccatgc tcaataaaga ccaattcgcg
    10621 gacaaccatt tcatccgcac catcatcgaa gacgacctca aaagcggcaa acacgaagcc
    10681 gtccaaaccc gttttccgcc cgaacccaac ggctatttac atatcggaca cgccaaatcc
    10741 atctgcctga acttcggttt ggcgtatatt tacgacggtt tgtgcaacct gcgtttcgac
    10801 gacaccaacc cggaaaaaga aaacgacgaa tacgtcaacg ccatcaaaga agacgtcgag
    10861 tggctcggtt tccattgggc gggcgaaccg cgtttcgctt ccgactattt cgaccggctt
    10921 tatgactacg ccgtcggttt aatcaaagac ggcaaagcgt atgtcgatga tttgacgccc
    10981 gaagaaatgc gagaataccg cggtacgctg accgaagcgg gtaaaaacag cccttaccgc
    11041 gaccgcagta tcgaagaaaa cctcgacctg ttcacgcgta tgaaaaacgg cgagttcccc
    11101 gacggcagca aaaccctgcg cctgaaaatc gacatggcgg caggcaacat caatatgcgc
    11161 gatcccgtca tctaccgcat ccgccgcgcc catcaccaca acaccggcga caaatggtgc
    11221 atctacccga tgtacgatta cacgcattgc atttccgatg ccatcgaagg catcacgcat
    11281 tccttgtgca cgctcgaatt tgaagcgcac cgtccgcttt acgactgggt gttggacaac
    11341 atccccgcgc cgcacgccac ccgtccgcgc caatacgagt tttcccgttt ggagcttttg
    11401 tacaccatta cctccaaacg gaaattgaat cagttggttg tggaaaaaca cgtttccggc
    11461 tgggatgatc cgcgtatgcc gaccatttcc ggtatgcgcc gccgcggcta cacgcccgaa
    11521 ggggtgcgcc tgtttgccaa acgcgccggt atttccaaat ctgaaaacat cgtcgacatg
    11581 agcgtgttgg agggtgcgat tcgcgaagag ctggaaaact ccgccccgcg cctgatggcg
    11641 gttttgaacc cgctcaaagt gaccctgacc aactttcaag ccggcaaaac ccaaagccgc
    11701 cgtgccgcgt tccatccgaa ccacgaagaa atgggcgatc gcgaagtacc tgtttcacaa
    11761 accatctaca tcgaagccga cgactttgcc gaaaatccgc ccaaaggctt caagcgtctg
    11821 attcccggcg gcgaagtacg cttgcgccac ggctatgtca tcaagtgcgg tgaagtcgtc
    11881 aaagacgaag caggcaatgt ggttgaactc aaatgcagca tcgaccacga caccttgggc
    11941 aaaaatccag aaggccgcaa agttaaaggc gtgattcact gggtttccgc cgaacacgcc
    12001 gccgaaatca aagtccgtct gtacgaccgc ctctttaccg tcgagcgtcc cggtgccgtg
    12061 cgcggcgaag acggcgaata cctgccgttt accgatttcc tcaatccgga atccgttaag
    12121 gaaatcactg cttacgccga acctgccgcg aaagatttgc cggcggaaag ccgttggcag
    12181 ttcgagcgca tcggctattt tgtgaccgac cgccaagacc acggcaaaga cacgccggtg
    12241 tttaaccgca cggtgacgtt gaaagattct tggcagccta agtaaacccc atccttgccg
    12301 tctgaatatt gttcgggcgg catttctcct ttacccgcga aatgcggcac attcggcaca
    12361 ccggaaagga aatatgatga aagtcctctt tatcgccgac ccgatggcaa gtttcaaaac
    12421 ctacaaagac accacctacg cgatgatgcg ggaaatggca aaacgcggct ggcggctgtt
    12481 tcataccttg agcggggaat tgtctgtaaa cggcggtttg gtaacggcac aggcatcggc
    12541 atttgaattt tcgggtgcaa aaaacgatga tgaccatgaa tggtttaaag cggcggacaa
    12601 agttcagacg gcattaaaag aatttgatgc cgtgattatg cgtaccgatc cgccgttcga
    12661 tatgcaatac ctttactcca cccaattact gacgctggcg gaacagcagg gcgcgaaagt
    12721 gttcaacagc ggacgggcga tgcgcgactt taacgaaaaa ctggcgattt tgaatttcag
    12781 ccgctttacc gcgcccacgc tggtaacgac ccgttccgcc gatgtccgcg catttttgaa
    12841 agaacacggc gacatcatcg tcaaaccgct cgacggcatg ggcggcatgg gcatcttccg
    12901 cctgaccgaa aaagacccca acatcggcag catcctcgaa accctgatgc ggtttgattc
    12961 ccgcaccatt atggcgcaac gctacattcc cgaaatcgta cacggtgaca aacgcatctt
    13021 gattatcggc ggcaaagtcg tcccctatgc tttggcgcgt atcccgcaaa acggcgaaac
    13081 acgcggcaat ctggcggcag gcgggcgcgg tgtggcgcag gaattggacg gacgcgaccg
    13141 ggaaattgca gagactctgg ctcccgagct taaacggagc ggcatcctgc tggccggttt
    13201 ggacgttatc ggcagcaacc tgaccgaagt caacgtaacc agcccgaccg gattccaaga
    13261 aattatgaaa caaaaaagtt tcgacgtggc ggcaatgttt gccgatgccg ttgccgcgtg
    13321 gtcggtacgt taaaccgatg ccgtctgaaa ggcttttgct tcgtaaccgc ttggtttgtt
    13381 cgggcaggca gggttttccc tcggccggca gggatgcgct taatgccgta cggacacccc
    13441 tccgccccgt tttcagacgg catatattga ggacattttg aaaggatacc gatggaacct
    13501 tcctcctacg cggcagaaaa aaaaggcaaa agcggcatca ggcgcgtcat caacgcattc
    13561 ggctattcga tagacggcat cgccgccgcc taccgttacg aagcggcatt ccgtcaggtt
    13621 ttgtggctga acgcgctgct ggtgtgcgcg gcattttttt gggtttccga aaccgccgtc
    13681 cgcctgccgt tgattatcgc gtcttttgtg tcggtcattg tcgaactgtt caacaccgcc
    13741 gtcgaagccg ccgtcgatca tacttcgacc gaaaaacacg agctggccaa acgcgccaaa
    13801 gatgcaggtt ctgccgcaca actggttgcc atgctgatgt tggcggcggt ttggctgtcc
    13861 gccctgttcg ggtaaaacgc ttgcagcagg atttgtaatc ctttaggatt agtatttatt
    13921 attcacttaa tctacatcaa atttccgagc agtatttgta tgtaagatta agcacattcc
    13981 ccgtctgata ttaaaggagc aggaagatga aaaaattatt ggcagccgtg atgatggcag
    14041 gtttggcagg cgcggtttcc gccgccggag tccatgtcga ggacggctgg gcgcgcacca
    14101 ctgtcgaagg tatgaaaatg ggcggcgcgt tcatgaaaat ccacaacgac gaagccaaac
    14161 aagacttttt gctcggcgga agcagccccg ttgccgaccg cgtcgaagtg catacccaca
    14221 tcaacgacaa cggcgtgatg cgtatgcgcg aagtcaaagg cggcgtgcct ttggaggcga
    14281 aatccgttac cgaactcaaa cccggcagct atcacgtgat gtttatgggt ttgaaaaaac
    14341 aactgaaaga gggcgacaag attcccgtta ccctgaaatt taaaaacgcc aaagcgcaaa
    14401 ccgtccaact ggaagtcaaa accgcgccga tgccggcaat gaaccacggt catcaccacg
    14461 gcgaagcgca tcagcactaa tctgctgaaa atatttgaaa tgccgtctga aaaagcccgg
    14521 gctttcagac ggcattttta tgcccgcctt taaaatgtgt taaaatccgc ctttaaaaac
    14581 cgccgtttcc aagccatcct gcgtatgaat acgacatcaa acacttccaa tatcatcgtc
    14641 gggctttccg gcggtgtcga ttcttccgta accgccgccc tgctcaagca gcagggttat
    14701 caagtgcgcg gtgtgttcat gcagaactgg gaaaacgacg acaacgacga atattgcagc
    14761 atcaaacagg attcgttcga tgccatcgcc gttgccgata ttgtcggcat cgacatcgac
    14821 atcgttaatt tcgccgcgca atataaagac aaagtttttg cttattttct tcaggaatac
    14881 agtgcggggc gcacgccgaa tccggatgtg ttgtgcaatg ccgaaatcaa attcaaatgc
    14941 tttttggact acgccgtagg gcagggcgcg gataccattg ccaccggaca ctatgcgcgc
    15001 aaagaagtcc gcaacggcgt gcattacctg ctcaaaggtt tggatcgaaa caaagaccaa
    15061 agctattttc tctaccgcct caagcctttc caactcgaac gcgcgatttt tccgttgggc
    15121 ggtttggaaa aacccgaagt gcgccgcctt gccgccgaat ttaatttgcc gactgccgct
    15181 aaaaaagaca gtaccggcat ctgtttcatc ggcgagcgtc cgttccgcga gtttctgcag
    15241 aaatacttgc cgaccgacaa cggcaaaatg gtgacgcccg aagggaaaac catcggcgaa
    15301 cacgtcgggc tgatgtttta cacattgggt cagcgcaaag gattgggcat cggcggcgcg
    15361 ggcgaaccgt ggtttgttgc ggctaaagat ttgacgaaaa acgaactcat cgtcgtacaa
    15421 ggacacgacc atccgctgct ctatacccgc agccttgtga tgaacgattt gagtttcacg
    15481 ctgcccgaac gtccgaaggc aggacgctat acctgcaaaa cgcgttaccg tatggcggac
    15541 gcgccttgcg aattgtgcta tttggatgat gaaaccgccg agctggtgtt tgacgaaccg
    15601 caatgggcgg ttacgccggg tcagtccgcc gtgctgtacg acgtcgacat ctgtttgggc
    15661 ggcggcatca tccaaacgac cgacaaaccc gtcatcatca cgcgataaag gtaatgccgt
    15721 ctgaaacggt tttcagacgg cattgttccg ctcaattcca ctttaaagac cgatacctat
    15781 ggaaaaaatc tggttagaaa gctacgagaa gggcgtcagt gccgaaatcg acatcacgca
    15841 atacaattcc gtcagcgacg tattccgcca aagcgtggaa aaatttgccc gtctgcccgc
    15901 ttttcaaaat atgggcaaaa cgctcactta tgccgaaacc ggcaaactgg cgaccgattt
    15961 cgcctcttat ctgcaaaacg tcctcaagct gccgcgcggc gaacgtgttg ccattatgat
    16021 gccgaacgta ttgcaatatc cgattgccct tttcggtatt ttgcaggcag gtttggtggc
    16081 ggtgaacacc aatccgctct atacgccgcg cgagttggag catcagctga aagacagcgg
    16141 tgcgaccgcc atcatcgttt tggaaaattt cgccaacacg ctggagctgg tgctgccgcg
    16201 cacgcagatc aaacacgtca tcgtcgcctc cgtcggcgaa atgttcgggc tgcttaaagg
    16261 ttcgctgatc aatttcatca tccgaaaaat caagaaaatg gttcccgaat accgtattcg
    16321 ggaaaccgtt tcctttcaga cggcattgaa agagggggcg aagcacgttt tccaacctgt
    16381 cgcattaaac cgcgaagata ccgcattgtt gcaatacacg ggcggcacga caggcgttgc
    16441 cagaggcgcg gtgctgagcc acggcaacat ctgcgccaat atgcttcagg caaaagaatg
    16501 gattaaaaac caattgcgcg agggaaaaga aaccgttatc gccgccctgc cgctgtacca
    16561 catcttcgcc ctgacagtga atctgatgat ttttgccaat gccggctcga aaattgtcct
    16621 gattgccaac ccgcgcgata tgaaaggctt tatcggcgaa ctgaaaaagc agcgggttaa
    16681 cgtatttatc ggcgtgaaca cgctgtttaa cgcgatggtc aaccggcccg atttcgccga
    16741 agtcgatttt tcaggattgc ggctgacttt gggcggcggt atggcgaccc aaaaagccgt
    16801 tgccgaaaaa tggaaaaaaa tcaccggcac gcccatcgtc gaagcctacg gtttgaccga
    16861 agccagcccc ggcgtgtgct gcaacccctt aaacatcgaa tcatacagcg gcagcatcgg
    16921 tttgcccgtc ccgtccaccg aagtcgaact gcgcgacgca aacggcaaag aagtccccgt
    16981 tgggcagccg ggcgaattgt gggtaaaagg cccgcaagtg atgcaaggct actggaaccg
    17041 ccccgaagaa accgccaaag ccatagacgc gtgcggcttt ttggaaaccg gcgatattgc
    17101 cgtgatggac gaaaaaggcc gtctgaagct ggtcgatcgc aaaaaagacc tcgtcgtcgt
    17161 ttccggattc aatgtttatc cgaacgaaat cgaggaattc atcgcgcacc acgaaaaagt
    17221 tatggaagtt gcgtgtatcg gcgtacccga cgaaaaaacc ggcgaggcac tcaaagtgtt
    17281 cgtcgtcaaa aaagacccgt ctttgatcaa agaagaactc accgctttct gccgtaccgg
    17341 tctgaccgca tacaaagtgc cgaaagacat cgaattccgc gacgagttgc ccaagtccaa
    17401 tgtcggcaaa atcctgcgcc gcgagttgcg ccaaagtgcc gggaaataaa gaaaagatac
    17461 cgtctgaaaa cagccgtcca ccgttcagac ggcatccgtc cgtttgcaag aaccgcgcgc
    17521 ttcacgttaa aatcacgcat tccaatacgg gtattccatc atgaccaaat tcattttcgt
    17581 aaccggcggc gttgtctcct cactgggtaa aggtatcgcc gccgcttcta ttgccgccat
    17641 cctcgaatcg cgcggcttga acgttaccat gctcaagctt gatccttata tcaacgtcga
    17701 tcccggcacg atgagcccgt tccaacacgg cgaagtgttc gtaaccgacg acggcgcgga
    17761 aaccgacctc gacttgggac actacgaacg tttcatcgat tccacgatga cccgccgcaa
    17821 cagcttcagc acgggtcagg tgtacgaaaa cgtcatcgcc aaagaacgac ggggcgacta
    17881 tttgggcggc acggttcaag tcatcccgca cattaccgac gaaatcaaac gccgcatcca
    17941 cgaaggcgcg gcgggttacg atgtggcgat tgtcgaaatc ggcggtacgg tcggcgacat
    18001 cgaatcgctg ccgtttttgg aagccatccg ccagatgcga agccagttgg gacgcaacaa
    18061 caccctgttc gcccacttga gctacgttcc ctacatcgcc gccgcaggcg aaatcaaaac
    18121 caagccgacc cagcacaccg tgaaagaaat gttgagcatc ggcttgcaac ccgacatcct
    18181 gatttgccgt atggacagga aaatgccggc agacgaacgc cgcaaaatcg ccttgttctg
    18241 caacgtggaa gagcgcgcga ttgtcggcag ctacgatgtg gacagcatct acgaatgccc
    18301 cgaaatgctg cacgaccaag gcatcgacaa catcattacc gagcagttgc agcttaacgt
    18361 gcagcaggcg gatttgaccg cgtggaaaaa aatcgtccac gccgtcaaaa acccgaaaca
    18421 caccgtcaaa atcgcgatgg tcggcaaata cgttgatttg accgaatcct acaaatcatt
    18481 gattgaagcc ttgaaacacg cgggcatcca taccgaaacc gatgtgcaga ttaccttcgt
    18541 tgacagcgaa agcatcgaga aaaacaaggg cgacgtttcc gtactcaaag atatggatgc
    18601 catcctcgtt cccggcggct tcggttcgcg cggcgtggaa ggcaaaatcg ccgccgtgcg
    18661 ctacgcccgt gaaaacaacg tgccatactt gggcatctgc ctcggtatgc agattgcgct
    18721 gattgaatac gcccgcgacg tggcaggttt gaaaggtgcg aattccactg agtttgactt
    18781 gaaatgcgct gcccccgtcg tcgccctgat tgacgaatgg caaaccgccg acggcagcgt
    18841 cgaaacccgt gacgaatccg ccgatttggg cggcacgatg cgtttgggcg cgcaagaagt
    18901 cgaattgaaa gcaggcagcc tcgccgtcaa aatctacggc agcggacaca ttcgcgaacg
    18961 ccaccgccac cgctacgaag tcaacaacaa ctacgtttcc gcgctggaac aggcaggttt
    19021 ggtcatcggc ggcgtatccg ccggacgcga acgcttggtc gaaaccatcg aactgccgaa
    19081 ccatccttgg ttcttcgcct gccagttcca tcccgagttc acgtccaacc cgcgcaaagg
    19141 gcatcctttg ttcaccgcgt ttgtcaaagc cgcgttgaac aataaaaaag cctgataaag
    19201 cgttacttga tgatcaaaat gccgtctgaa agcctgacaa agactttcag acggcatttt
    19261 cgcaaatcgg ggatcataaa agccatcaat ttaatt
//
LOCUS       DAXDXW010000040        18642 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXDXW010000040 DAXDXW010000000
VERSION     DAXDXW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18642
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.041"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            4..628
                     /locus_tag="ACFB64_001196"
                     /pseudo
     CDS             4..628
                     /locus_tag="ACFB64_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448870.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            664..1425
                     /locus_tag="ACFB64_001197"
     CDS             664..1425
                     /locus_tag="ACFB64_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC1209560.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIS
                     ALDFAMVKVPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGGLTNVEQGESVCV
                     PVGDISDWMFVCNGIPYGGFTVQAMRGQMTEEERTEHDTAWGIDFGDPGQVLPVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAMAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            1481..1822
                     /gene="fdx"
                     /locus_tag="ACFB64_001198"
     CDS             1481..1822
                     /gene="fdx"
                     /locus_tag="ACFB64_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC1209561.1"
                     /translation="MPKITVLPHATLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPAELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(1882..2421)
                     /locus_tag="ACFB64_001199"
     CDS             complement(1882..2421)
                     /locus_tag="ACFB64_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFC1209562.1"
                     /translation="MNNMFAAKLSELVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHTLYRVTVCLNYRSRRGLIALDKAAVGCDAAMLRARRWRICMQIAERLSESVGRLH
                     GCGRKREAACPELEGRYLDLVRRAAVSFGFAQGQANPAGGQEETQAASDAVSGRSVKK
                     PIKINIESKVPSSQTSVFR"
     gene            2634..2831
                     /gene="iscX"
                     /locus_tag="ACFB64_001200"
     CDS             2634..2831
                     /gene="iscX"
                     /locus_tag="ACFB64_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC1209563.1"
                     /translation="MKWTDTRRIAEELYDLHGEAIDPKTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(2899..3300)
                     /locus_tag="ACFB64_001201"
     CDS             complement(2899..3300)
                     /locus_tag="ACFB64_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC1209564.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPHKIKVKGLNHQRRPAPEARLLYEEDAKTAALREECKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            3512..4471
                     /locus_tag="ACFB64_001202"
     CDS             3512..4471
                     /locus_tag="ACFB64_001202"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC1209565.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVVVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            4539..5849
                     /gene="tilS"
                     /locus_tag="ACFB64_001203"
     CDS             4539..5849
                     /gene="tilS"
                     /locus_tag="ACFB64_001203"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC1209566.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVCA
                     GKRAGFVPEALHIHHGLSPRADDWADFCRNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAEFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPLGENGIIWR
                     PLLPFSRQDIWDYARKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLEEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRNFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFASEQLAKPAFLKDGTISGNLK
                     KILTEHRFVLKRHPFGLPEAMLEQDGILRTVAASDTLAVGGIHKNVKKILQGKRVLPF
                     LRPIWPLVADSGNRPLALANCCADFQISVSDGILPVHPDFPILF"
     gene            5923..6708
                     /locus_tag="ACFB64_001204"
     CDS             5923..6708
                     /locus_tag="ACFB64_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC1209567.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEAKMPSEEVLKLTAVLPEDGIFSVSDGILKKISSLSCADEILTLIDI
                     PLGGTLPDKGDCAVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLLDIYSQADLEIWLAHYEDRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            6718..6915
                     /locus_tag="ACFB64_001205"
     CDS             6718..6915
                     /locus_tag="ACFB64_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209568.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILSSNAVAAAVLFWIWWKWF"
     gene            7177..7530
                     /locus_tag="ACFB64_001206"
     CDS             7177..7530
                     /locus_tag="ACFB64_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209569.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLP
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEEQ"
     gene            7542..7847
                     /locus_tag="ACFB64_001207"
     CDS             7542..7847
                     /locus_tag="ACFB64_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC1209570.1"
                     /translation="MRVSKMIGSILLVAAVQTVFSANVYECRHNGKTSYSQTPGKDCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEAVQNPKGNAQKDGSDAGIRPH"
     gene            7909..9285
                     /gene="mpl"
                     /locus_tag="ACFB64_001208"
     CDS             7909..9285
                     /gene="mpl"
                     /locus_tag="ACFB64_001208"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC1209571.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFKVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFQADIYVIGNVARRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SKSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGQQQSLQDTLDKGCWTPVEKFGTGHGWQIGEVNADGSFDVLLDGK
                     KAGHVAWDLMGGHNRMNALAVIAAARHAGVDVQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPASLKEADQ
                     VFCYAGGADWDVAEALAPLGCRLRVGKDFDTFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            9492..10544
                     /gene="bioB"
                     /locus_tag="ACFB64_001209"
     CDS             9492..10544
                     /gene="bioB"
                     /locus_tag="ACFB64_001209"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC1209572.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVYQAA
                     EVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTVSVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKADY"
     gene            complement(10640..11413)
                     /locus_tag="ACFB64_001210"
     CDS             complement(10640..11413)
                     /locus_tag="ACFB64_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC1209573.1"
                     /translation="MQTTKICYAFRTPSAYGQYLADAGFDYLSLANNHSNGFGAQGIT
                     ATAGNLDELNIKYSGIENRFETAILKKNGVRYGFVSFAPNLAAVKLNDYAKFKKLIRK
                     TKQKTDIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGADVVFGQG
                     AHVTRAVELYHDRFISYSGGNFATYGISGIAPIFKIITDKQGRFVSGNIIPITQVGDK
                     IPKIDPEKTVIERIIYLNHSDFPNGNGLDVSPGGDITRR"
     gene            complement(11451..11753)
                     /locus_tag="ACFB64_001211"
     CDS             complement(11451..11753)
                     /locus_tag="ACFB64_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209574.1"
                     /translation="MIINKFIWAMAVFSAILALVIGSGFTALEYVKEPPAAPYAVSAP
                     KTAGVKPRRILPERNPPCCRLTVQTPRPFQTPAMRTDRIRLTERQQIPFPSSGSAA"
     gene            complement(11795..13654)
                     /gene="ilvD"
                     /locus_tag="ACFB64_001212"
     CDS             complement(11795..13654)
                     /gene="ilvD"
                     /locus_tag="ACFB64_001212"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC1209575.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQDDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVYTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRIWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEAAVEDILGNQIVAGNIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTIEIDIPKRSIRLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVSQIER"
     gene            complement(13827..15592)
                     /gene="cysI"
                     /locus_tag="ACFB64_001213"
                     /pseudo
     CDS             complement(13827..15592)
                     /gene="cysI"
                     /locus_tag="ACFB64_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
     gene            complement(15619..17412)
                     /locus_tag="ACFB64_001214"
                     /pseudo
     CDS             complement(15619..17412)
                     /locus_tag="ACFB64_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
     gene            complement(17409..18491)
                     /locus_tag="ACFB64_001215"
     CDS             complement(17409..18491)
                     /locus_tag="ACFB64_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC1209576.1"
                     /translation="MNYFPIFANLSGRPVLVVGGGAVAARKISLLPKAGAEVGVAAKH
                     LNAELSALAAENKILWLAKEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VGDRDHCSFIFPSVIDRDPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAGFYFPVQLVVRQDADKADGFRGYQGRIERGSVTVGQTVRIEPNGLTAEVSEIIA
                     PKGEVAQAFAGEAATIRLDRDIDVSRGDLFVDKNFPLAPQKHPEATLCWFDERPLNTA
                     RKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRINLQKPVTATPY
                     AENTAAGSFILIDEATYGTVAAGMIL"
BASE COUNT         4113 a         4787 c         5351 g         4391 t
ORIGIN      
        1 gtcctgattt ttgttaattc actatataaa atcggcattt cggacgtaag caattttgaa
       61 ggcagaatgc gccatttgga aaacaacggc tatgcgaacg ttgccgggtt ggaacgcctc
      121 ctcgccgtca aaaccgacaa ttacaaagaa aaaaaacctg ctccatgaaa ttttcagcaa
      181 aagcaggata ggcgataccg aattgttcgc cgtggacgaa aaccttgtga aacgtttgtt
      241 tttatcgctt cgcggcgaaa tcgtgttccc gaaaaacgaa acggcggaat cggaatttga
      301 aaaaagcgtc cacgaacgcc ggcaggaagg gaatgccggg tccggccgca aacaactgct
      361 tgatttggta cggcgcggac accgggaata cccttacgcg ctgccccggc ttttggcgga
      421 cgcggcatcc tacaagccga aaaaatcgaa aatccgcctt tttaaagaag catatttcgg
      481 caaaagcggc acgaggctga ccgacgaaat tgcagacggc atccatattt acacctgttt
      541 ttcgcgggcg gatttggaaa aagcctattc cgaatatttg gaacttttca aatccgaatc
      601 ggatgccgga ggtagaaaac cgcggtaagg tgcaaacaga taccgtacac gttgaggagc
      661 agtatgatgg gcgattccgt catttattat gtagaacagg cagacgaacc ggtaaaccgt
      721 gccggcgaac gcgcccgtaa aacattcaaa tacttttggc gcgagctttt ttgggaacgc
      781 cgccgcatta tttccgcctt ggattttgcc atggtcaaag tccctttttt ccaagacggc
      841 gaagacggcg aaatttgcga acatatgtgg atcgacgata tttattttga cggtctttat
      901 atttacggtg tgctgaacaa tgaacccggc ggactgacca atgtcgaaca aggcgaaagc
      961 gtttgcgttc cggttggcga catcagcgac tggatgttcg tgtgcaacgg catcccctac
     1021 ggcggcttta ccgtacaggc aatgcgcgga cagatgacgg aagaggagcg taccgaacac
     1081 gataccgcct ggggaatcga tttcggcgat cccgggcagg tattgccggt gtatgaagaa
     1141 aaagaacatc ccgaaaattt ggaagagcat ccgatgtgcc ggaactgtat tgacgatttt
     1201 cggcaacagt tgtcccaaaa tccggatttt ctgcatgagc aggacgaaga cggctatacg
     1261 ccgcttcatc atgaagccat ggcaggaaat gcacttatgg ttcaagccat gcttgaatac
     1321 ggcgcaaatc ctgcctcaaa gacatcggaa ggctataccg ccctcgattt tgcccgcctg
     1381 acgggctggc aaaatgttgc cgacctgctc gaaccgcgac attaggcaga cagttttcct
     1441 aaaaacggac acaaatactt tttacagaaa gacaataaaa atgcccaaaa tcaccgtact
     1501 tccacacgca acattatgcc ccgagggtgc agtcatcgat aacgcgcccg aaggcaaaac
     1561 cgtcctcgac gtgctgctcg accacgacat cgaagtcgat cacgcctgcg aaaaatcctg
     1621 cgcctgcacc acctgccacg tgattatccg caaaggtttc gacagtttgg aagagccggc
     1681 cgaattggaa gaagacctgc tcgatcaggc ttggggtttg gaagccgatt cgcgcctgag
     1741 ttgtcaggcg gttgttgccg gcgaggattt gattgtggaa atccccaaat acaccatcaa
     1801 ccacgcgcgc gaagaacact gaaaacaggc cgtctgaagc cggcacgctt cagacggcat
     1861 tgttgcgcgg ataaggcgca attaccgaaa aacagacgtt tgtgaagacg gaactttcga
     1921 ttctatattg atttttatcg gttttttgac ggaacgaccc gatacggcat ctgaggcggc
     1981 ttgcgtttct tcctgcccgc ctgccggatt tgcctgtcct tgcgcgaaac cgaaagagac
     2041 ggcggcgcgg cggacaaggt cgagatagcg tccttcaagc tcggggcagg cggcttcacg
     2101 ctttctgccg caaccgtgaa gccgcccgac cgactcggaa aggcgttccg caatctgcat
     2161 gcagatgcgc caacgccggg cgcgcaacat tgccgcatcg caaccgacag ccgctttatc
     2221 caaagcaatc agaccgcgcc tgctccggta gttaaggcaa accgttactc tgtagagggt
     2281 atgttgcccg tccaatattt gatacagttt gcgtatcagc agaatcaggc ggtcaaactc
     2341 ctccatctcc gacagggagt cgggatggtc ggaagcggta taaaccagtt cggacaattt
     2401 tgcggcaaac atattgttca tcaatcttcc ttatcggttg aaaccccgct cttcagggcg
     2461 gtagaatcag atttgtttgg gaggggtaac tcctcccgga tcaggacgac acataaggtg
     2521 gtgcttgatg tgccgtccgg cgagttgaaa cattcagcca tcctcaaggg gcggcagttt
     2581 tgccgaaaca tattctacac ggcttcaatg ccggacgata aaaggaaatt tatatgaaat
     2641 ggaccgatac ccggcgcatc gccgaagaac tctacgacct gcacggcgaa gccatcgatc
     2701 ccaaaaccgt gcgctttacc caactgcgcg acctgattat ggcattgccc gaatttgacg
     2761 acgatcccgc ccgttgcggc gaacgcatcc ttgaagccgt gcagcaggca tggatagacg
     2821 aggcggaata agtttcagga atgccgtctg aaatgcggcg gtacgcggtt cgtgcttctg
     2881 tttgcagcgg gaatggtttt accagtctcc ttttttcagc ctgtccagtt ggcggcggtc
     2941 gcgcttggtc ggtctgccgt cgggatgggc ggaagtgatg cggctgaatt ggtcgagctg
     3001 tttgcactct tccctcaatg ctgccgtttt cgcgtcttct tcatacagaa gccgcgcctc
     3061 gggtgccggg cggcgttggt ggttcaaacc tttaaccttg attttatggg gaagggaatt
     3121 gagcgtcagg tcgataatat cgccgatgtc tatggtttta ctgtttttga ctttcgagcc
     3181 gtttacttga accctaccca gttcgatatg cttttgcgca agggaacggg tcttgaaaaa
     3241 acgtgccgcc caaagccatt tgtccagccg catggcggaa gaatcgtgct tgtctttcat
     3301 acgattttgt ttgaaataat tgaatttgtt tcgagtttag cataagatac atcgccttat
     3361 aactagtata tatgcactaa tccgctgttt tccatgctgt ccgaacacaa aaagagggtg
     3421 tggaaaagcc gttttggaca ataaattaac tgcggaatat gcacaaatag cgtatgatag
     3481 cggcagaatc tgttgatgag agcttcattt tatgaaacct gtttttttgg attttgaaca
     3541 acccatagcc gaactgacca acaaaatcga tgagctgcgt ttcgtccaag acgagtctgc
     3601 cgtcgatatt tcggacgaaa tacaccgttt gcagaaaaaa agcaacgatt tgaccaaatc
     3661 gattttcagc aaactcacac ccgctcaaat ttcacaggtt tcccggcatc cgcagcgtcc
     3721 ctatactttg gattacattg acgcgctgtt taccgatttt gaagagctgc acggcgaccg
     3781 ccactttgcc gacgatcatg cgattgtcgg cggattggcg cgtttcaacg gacaaagcgt
     3841 tgtggtcgtc gggcatcaga aagggcgcga caccaaagaa aaaatccgcc gcaacttcgg
     3901 tatgccccgt cctgaaggct accgcaaagc cctgcgcctg atgaaaacgg cggaaaaatt
     3961 cggcttgccc gtcatgacct ttatcgatac gccgggcgcg tatcccggca tcggcgcgga
     4021 agaacgcggg cagtcggaag ccatcggcaa aaacctgtac gaactgaccc gactgcgcgt
     4081 tcccgttttg tgtaccgtca tcggcgaagg cggttcgggc ggtgcgttgg cggtcgccgt
     4141 aggcgattac gtcaatatgc tgcaatattc gacctattcc gttatttccc ccgaaggctg
     4201 tgcttcgatt ttgtggaaaa ccgccgaaaa ggcggctgat gcggcacagg ctttgggtat
     4261 tactgccgac cgcctgcaaa agctggactt ggtcgatacc gtcattaaag agcctttggg
     4321 tggtgcgcat agggatttcg ggcaaaccat gaaaaacgta aaagccgttt tggaaaaaca
     4381 actgcacgaa gcgcaaagca tcccgcttgc cgatttgctt tcgcgccgtt tcgaccgcat
     4441 tatggcttac ggcaaatttt cggaacaata attcgggtag aacaagcagc aagcagtttg
     4501 tctgaaactg cttgcttttt ctttatcggg atgaagccgt gctgacttta gatgcgtttg
     4561 agcaatgctt gaaggattgt tttcctcaag gtctgaatgg aaaaaaaaca gcagtggcat
     4621 taagcggcgg cttggattcc gtcgttttgc tgcatctgct ggtttgtgcc gggaagcggg
     4681 caggatttgt gccggaggca ttgcacatcc atcacggctt gagtccccga gccgacgatt
     4741 gggcggattt ctgccggaat tattgcgata tgctaggggt ggggctggaa acggttaagg
     4801 tctgcgtgga aaaaaacggt ttgggcatcg aggcggcggc aaggcaaaag cgttatgccg
     4861 agtttgccga aaaaggcttt gacgttttgg cgttggcgca ccacagggac gatcaaatcg
     4921 aaacctttat gctggcagtc gcgcgcggcg gaggtttgcg cgctttggcg gctatgcccg
     4981 ccgtccgccc tttgggggaa aacggcatta tctggcggcc tttgctgcct ttttcgcgcc
     5041 aagacatatg ggattatgcc cgaaaacacg gtttgccgaa tatcgaggat gaaagcaata
     5101 ccgatacggc ttatttgcga aaccgcttcc ggcaccgtat tttgcccgaa ctttcggcgc
     5161 agattcccca tttcgggcgg catgtgctga acaatgtccg cgctttgcag gaagatttgg
     5221 ctttgttgga agaggtcgtc gttcaagact gccgttgggt ttgcggggcc ggttatttcg
     5281 atacggcgcg gtggctgacg ttttccccgc gccggaaaac ccatattttg cggaattttc
     5341 taaaggaaaa cggcatcccc gtgccgaatc agaacgcact tgccgacatt gcccgcgttt
     5401 tgacggaggc aaaaaccgga cgttggaact tgcaaggctt tgaattgcat cattatgcag
     5461 gcaggctgtt tgtgtttgcg tcggaacagt tggcaaaacc ggcttttctg aaagatggga
     5521 cgataagcgg aaatctcaaa aaaatactga cagaacatag atttgtgttg aagcggcatc
     5581 cgttcggact tcctgaagcc atgttggaac aggacgggat tttgaggacg gtagcggcat
     5641 cggatacgtt ggctgtcggc ggcatccata aaaatgtgaa aaaaatcctt caggggaaac
     5701 gggttctgcc tttcctgcgc ccaatttggc cgctcgttgc cgacagcgga aaccgtccat
     5761 tggcgttggc aaactgttgt gcggattttc aaatctcggt ttcagacggc attttgcccg
     5821 tacatcctga ctttcccatt ttattttgat aatatcgcaa acagatttcg gcggcgttca
     5881 gtcgggtatt gtccggttgc atatttctaa aaggcttgtg aagtgaaaca catcagttcg
     5941 accaataatg aacatatcag acacctgcac cgcctgttgt cgcaaggaaa gttcagacgg
     6001 cagtatgccc aaaccgtttt ggagggcgtg cacctgcttc aggttttcct gcaatccggc
     6061 agaaagccgg tcggggtata tattcccgaa gctaaaatgc cgtctgaaga agtccttaaa
     6121 ttgacagcgg ttttgccgga agacgggatt ttttccgttt cagacggcat attgaaaaaa
     6181 atcagcagct tgagttgtgc agacgagata cttacactga ttgatatccc attgggcgga
     6241 accttgccgg acaaaggcga ctgtgcggtt ttggacggcg tgcaagatcc gggcaatgtc
     6301 ggcaccgtct tacggagtgc ggcggcggca ggggtaggta cggtcgtttt gggcaggggt
     6361 tgtgcggacg cgtggtcgcc caaggtatta cgcgcgggta tgggcgcgca tttcttgttg
     6421 gacatttatt cgcaggcgga tttggaaata tggttggcgc actatgaaga ccgtgtgttt
     6481 gccaccgcgt tgcgcgagga aaagcaggcg gttttgtacg gcgaagattt gtgcgaaccg
     6541 acagcttggg tgttcggcaa cgaaggtgcg ggtgtcggta aagcagtttt agacagggcg
     6601 gacaagtgtg tcaggatacc gatgcacgat gcaaccgagt ctttaaatgt cgcgatggcg
     6661 gcgacaatct gcctgtttga acagatgcgc caacgggcgg cgtattgagg aagagaaatg
     6721 ccgtctgaaa aaatctatta cggcgtattg attttcttat gtatcgcttc tatgctgctg
     6781 tcaccgtttt tttatgcggg tgctttgaag cccaagaagg cggcattgcg gaaggacggg
     6841 cagtggaaac tcatcttatc gtccaatgcc gtggcggcgg cggttttgtt ttggatatgg
     6901 tggaaatggt tttgacagat attgctcaaa atcgtgctaa tggaatccga acaaataaag
     6961 aatttggtaa aaaatttgtt aaatcaacgg attaaagttt tgtggaaaac aaaacagctc
     7021 taagcaaata gggcgtttgt cggtaaatac ggaagagttg cggcattatc gggcatcttt
     7081 aacaagtagt gtcggcttgg caggcaatcg gtttttatgg gcagcttgca aaatcgcgga
     7141 tataaaattg cgaatcggtt aaagtgtggg gacgctatga aaaattgcga atttttttat
     7201 gatccgacaa gggcaatcta tgatagcggt gcagattact taactaggga aaaacataga
     7261 ttagtcgtaa tcgcaaatag cgcttggggg ctattgctga atttaccttg ttattatgac
     7321 gaggttttgg aaaagcggaa aataccgttc ggcaaacagg aaattgatga cgatatggac
     7381 aaagtgtccg cccttaagcg gaagtttaaa gacatttctg aaatcaaagt aggggatggt
     7441 tgggaatacc cgttcaatta tgagcaagga atgaaagaat tagatgaagt tctattgaaa
     7501 tacattcctt tttttgaaga ggaacaataa aggagtttaa tatgcgcgta tctaaaatga
     7561 ttggaagtat attgcttgtt gcagcggttc agaccgtatt ttcggcaaat gtttacgagt
     7621 gccgccataa tggtaaaacc agttacagcc aaactccggg aaaagattgt accaacgcgg
     7681 gtttggggcg ggatcgggtg tacagttcgg tcaggcctgc cgtgaaagac agggcggaag
     7741 acgcaggagt tggcgattat tcggacacgg tgagggacga agccgtccaa aatccgaaag
     7801 ggaatgcaca gaaagacggt tcggatgccg gcatcagacc gcattgattg aaaccgaatc
     7861 aaccattacg gtgttgaaca atagaatttg aacgattagg gaactctgat gaaacacatc
     7921 cacattatcg gtatcggcgg cacgtttatg ggcgggattg ccgccattgc caaagaagcc
     7981 gggttcaaag tcagcggttg cgacgcgaag atgtatccgc cgatgagcac ccagctcgaa
     8041 gccttgggca taggcgtaca cgaaggcttc gatgccgcgc agttggaaga atttcaagcc
     8101 gatatttacg tcatcggcaa tgtcgccagg cgcgggatgg atgtggtcga ggcgattttg
     8161 aaccgtgggc tgccttatat ttccggcccg caatggctgg ctgaaaacgt gctgcaccat
     8221 cattgggtac tcggcgtggc agggacgcac ggcaaaacga ccaccgcgtc catgctcgcc
     8281 tgggtcttgg aatatgccgg actcgcgccg ggcttcctca tcggcggtgt accggaaaat
     8341 ttcggcgttt ccgcccgcct accgcaaacg ccgcgtcaag acccgaacag caaatcgccg
     8401 tttttcgtca tcgaagccga cgaatacgac accgcctttt tcgacaaacg ctccaaattc
     8461 gtgcattatc gcccgcgtac cgccgtgttg aacaatctgg aattcgacca cgccgacatc
     8521 ttcgccgact tgggcgcgat acagacccag ttccaccacc tcgtgcgcac cgtaccatcc
     8581 gaaggcctca tcgtctgcaa cggacagcag caaagcctgc aagatacttt ggacaaaggc
     8641 tgctggacgc cggtggaaaa attcggcacc ggacacggct ggcagattgg tgaagtcaat
     8701 gccgacggct cgttcgacgt attgcttgac ggcaaaaaag ccggacacgt cgcatgggat
     8761 ttgatgggcg gacacaaccg catgaacgcg ctcgccgtca tcgctgccgc acgccatgcc
     8821 ggagtcgatg ttcagacggc ctgcgaagcc ttgggtgcgt ttaaaaacgt caaacgccgc
     8881 atggaaatca aaggcacggc aaacggcatc accgtttacg acgatttcgc ccaccacccg
     8941 accgccatcg aaaccacgat tcaaggtttg cgccaacgtg tcggcggcgc gcgcatcctc
     9001 gccgtcctcg agccgcgttc caacaccatg aaactcggca cgatgaagtc cgccctgccc
     9061 gcaagcctca aagaagccga ccaagtgttc tgctacgccg gcggcgcgga ctgggacgtt
     9121 gccgaagccc tcgcgccttt gggctgcagg ctgcgcgtcg gtaaagattt cgataccttc
     9181 gttgccgaaa ttgtgaaaaa cgcccgaacc ggcgaccata ttttggtgat gagcaacggc
     9241 ggtttcggcg gaatacacac caaactgctg gacgctttga gatagcccgg gcaatgccgt
     9301 ctgaaagccc tttagacggc attgcccggc tgcgcggcac aaaggcggaa aaaccgtttg
     9361 ccccgtattt tcaaacgcgt tacacttgcc gccgctgttt tcagccattt gattacccgc
     9421 aaccgccgtc attgcgccgg cggtttgcct gtcagcgtca ttgcgccgct gtaaatacga
     9481 aagaacacat tatgaccgta tcccccgtcg ccttgcgccg taagaccgag tgcaagcctc
     9541 atcccaccgc gcgctattgg aaaaaatgcg atgtcgaggc acttttcgga ctgccgtttt
     9601 tagaacttgt ctatcaggcg gcagaagtcc accgccaaaa tttcaacccg cgcgaaatcc
     9661 agctttccac gctgttgtcc atcaaaaccg gcggttgtcc cgaagattgc gcctactgtc
     9721 cgcaatcagc gcaccacaac accaatttgg gcaaagagca gatgatggat gtggatgaaa
     9781 tcgtcgaaaa agccaaaatc gccaaatcgc gcggcgcaag ccggttttgt atgggcgcgg
     9841 catggcgcgg ccccaaaccc aaagacgtgg agacggtttc cgcaatcatc aaagccgtca
     9901 agggcttagg tatggaaacc tgcggcacgt tcggtatgct cgaagaaggt atggcggaag
     9961 acttgaaaga ggcgggcttg gattattaca accacaacct cgacaccgac cccgaccgct
    10021 acaacgacat catccacaca cgccggcacg aagaccgtat ggataccttg ggcaaagtcc
    10081 gcaacgccgg tttgaaagtc tgctgcggcg gcatcgtcgg gatgaacgaa acccgcgccg
    10141 aacgcgccgg gctgattgcc agcctcgcca acctcgaccc gcagcccgaa agcgtgccga
    10201 ttaaccggtt ggtcaaagtg gaaggcacgc cgcttgccga tgccgaagat ttggattgga
    10261 ccgaatttgt ccgtacggtt tccgtagcgc ggattacgat gccgcaaagt tatgtccgcc
    10321 tctcggcagg gcgcagcaat atgcctgaag cgatgcaggc gatgtgcttc atggcgggtg
    10381 cgaactcgat tttttacggc gacaagctgt tgaccacagg caatcctgat gaggacggcg
    10441 atagaatcct gatggaaaag cttaacctgt atcccttgca gtttgagccg gaaggcgagg
    10501 tcgccgaagt ggaaaaagcc tccgggatta aagcggatta ttgacgattg aaaaatgccg
    10561 tctgaaactt gaatgccgtc tgaaacctga aaaaaggctt tcagacggca tttgttcgga
    10621 cggaatttcc aatatctttt taccggcgcg tgatgtcgcc gccgggcgag acatccagtc
    10681 cgttcccgtt ggggaagtcg ctatggttca gataaataat ccgctcaata acagtttttt
    10741 cagggtcgat tttggggatt ttatcgccga cttgagtgat ggggataatg ttgccggaaa
    10801 cgaaccgccc ctgtttgtcg gtgataattt tgaaaatcgg cgcaatgccg ctgatgccgt
    10861 aggtggcaaa gttgccgccg ctgtaagaga tgaagcggtc gtggtaaagt tcgacggcgc
    10921 gagtaacgtg cgccccctgc ccgaatacga catccgcgcc ggaatcgacg gcgagccgcg
    10981 caaactcgac gacgttgccc ctgttttccc catagaagat ttcggtatcg aacggcaggt
    11041 gttcagcctg tttcccttcc gcgccgccgt ggaacatcac aatgacgatg tcggtttttt
    11101 gtttggtttt ccgaatcagt tttttgaatt tggcgtaatc gttcagtttg acggcggcaa
    11161 ggttgggggc gaaggagacg aagccgtatc tcacgccgtt tttcttcagg atggcggttt
    11221 caaacctgtt ttcgatgccc gaatatttga tgttcaattc gtcaaggttg cctgccgttg
    11281 ccgtgatgcc ttgcgcgccg aagccgttgc tgtggttgtt tgcgaggctg aggtagtcga
    11341 atcccgcgtc ggcaaggtat tgcccgtatg cggagggcgt tcggaatgca tagcatattt
    11401 tggtggtttg cacatttttt cggcgtaccg ccttcgtcaa acagcgtacc ttatgccgcc
    11461 gaccccgatg atggaaacgg tatctgctgc ctttccgtca ggcgtatcct gtccgtccgc
    11521 atcgccggtg tttgaaacgg gcgcggggtt tgaaccgtca atcggcagca cgggggattc
    11581 cgttccggca ggatgcgccg aggcttgacc ccggcggttt tcggcgcgga aacagcataa
    11641 ggggcggcag gcggctcttt tacatactcg agcgcggtaa agccgctgcc gataacgagg
    11701 gccaaaattg cggaaaatac cgccatcgcc cagataaatt tgttaataat cataccttta
    11761 ctgttcagac ggcatttgcc gcacgttttg gggcttatct ctcgatttgc gatacgtcgc
    11821 gcaccgcgcc tttgtcggcg gaagtcgcca tcgcgccgta agcccttaat gcggcggaga
    11881 cgtagcggtc gcggttttcc ggtttccacg ctttgctgcc gcgagcttcc atttcggcac
    11941 ggcgtgcggc aagctcttca tcggaaatga caaggcggat gctgcgtttg gggatgtcga
    12001 tttcaatcgt atcgccttcg tgtaccaaac cgattgcgcc gccttccgcc gcttcgggcg
    12061 aggcgtgtcc gatggacaaa cctgatgtgc cgccggagaa gcgtccgtcg gttaagaggg
    12121 cgcaggcttt gcccaagcct ttggatttca ggtaggaggt cgggtatagc atttcctgca
    12181 tacccgggcc gcctttcggg ccttcgtagc ggatgatgac gatgttgccg gcgacgattt
    12241 ggttgcccaa aatatcttcg acggcggctt cctggctttc aaacacgcgc gcgcggccgg
    12301 tgaatttgag gatgctctcg tccacgcctg cggtttttac cacgcagccg cgctcggcga
    12361 tgttgccgaa taagaccgcc aaaccgccgt cttgcgagta ggcgtgtgcc acgtcgcgga
    12421 tacagccttt ttcgcggtca aggtcgaggg ttttccaaat acggttttgc gagaacgcct
    12481 gagtggtgcg cacgccgccg ggagccgctt tgaaacgttc gatggcgcgg gtgttttcgg
    12541 gattggtgac gtcccatttt tcaatcgcgt ctttcagtgt cggcgcgtgg atcgtgtaca
    12601 cgtcggtgtg cagtttgccc gctttgtcaa gttctttcag aatggcgaag atgccgccgg
    12661 cgcggtgcac gtcttccata tagtagtcgt ggttgttggg cgcggttttg cagatgcagg
    12721 gcacgacgcg gcttaagcgg tcgatgtctg ccattttgaa atccacgccc gcttcgttgg
    12781 cgacggcgag caagtgcagg atggtattgg tgctgcctcc catcgcaata tccatcgtca
    12841 tggcgttttc aaacgctttt ttggtggcaa tgctgcgcgg cagcacggtt tcgtcgtctt
    12901 gctcgtaata gcgtttggtg atttcgacaa tcatacgccc tgcttcgagg aacaattctt
    12961 tgcggcctgc gtgggtcgcc aaatacgaac cgttgccggg cagggacaga ccgagcgctt
    13021 cggtcaggca gttcatcgag tttgccgtaa acatacccga acacgagccg caggtcgggc
    13081 aggcgttttg ttcgacttct tcgacttgcc ggttgctgac attgtcgtcc gccgattcga
    13141 tcatggcgtc aatcaagtcc aaacggcgtt cgggttggat gtttgccacg ccgatgacct
    13201 tgcccgcttc catcgggccg ccggagacga agatggtggg gatgttcagg cgcatggcgg
    13261 caatcagcat tcccggggtg attttgtcgc agttggaaat gcacaccagc gcgtcggcgc
    13321 agtgggcgtt gaccatatat tcgatggagt cggcaatcaa atcgcggctg ggcagggagt
    13381 acaacatacc gctgtgcccc atggcgatgc cgtcgtcgat ggcgatggtg ttgaattctt
    13441 tggcaattgc acctgctttt tcaatctcgc gggcaaccag ctggcccata ttgtgcaggt
    13501 ggacatggcc gggcacgaat tgggtgaagg agttggcaac ggcgatgatg ggcttgccga
    13561 agtcggtttc catcacgccg gtggcgcgcc acaatgcgcg cgcgcccgcc atattgcggc
    13621 cgtgggtgga ggttttggag cggtattctg gcatagtgtg tttccttgtg cctataccgt
    13681 ctgaaaggca gggctgtttc agacggcatc gggtacggtt ttttagagtg ggaaaagggg
    13741 gtattttata ccaagtatcg gaattttgcg gaatggaaac gtgtgcggca aaaaagaaca
    13801 tccccgcagg aatgcggggg acgggttcag gcgcgcgcaa tcgggacggc tttggatgcg
    13861 tcccaaaaat cgacgggcgc atccaacacg ggtttgacga tgcccgtccg tatggcgaaa
    13921 tcgccgaaac cttcgtcggt atcgcgtccc gccgcccatt tgccgatcag gtcttccaat
    13981 tcggaaagga tttccggcag ggtgatgttt tctttgtaga ggcgggggat gcgtacgcct
    14041 tcgcggtcgc cgccgatgtg gaggttgtag cgtcccacgg ctttgccgac cagtccgatt
    14101 tccgccaaca tcgcccgtcc gcagccgttc gggcagccgg taatgcgggt aacgatgtag
    14161 tcgtccgacg cgccgtgttc cgccataatc ttatccagct cgccgatgaa gtccggcaac
    14221 acgcgttcgg cttccgccat tgccagcggg caggtcggga aggaaacgca cgacatcgca
    14281 ttttcgcgca gcttgctgac accgtcgcgt atcagcccgt atgagcgggc gagctgttcg
    14341 atccgggctt tgcctccttc gggcacattt gccacgatga ggttttggtt ggcggtgatg
    14401 cggaagccgc ctttgtggat tttggcgatt tccaacacgc cggtcagaag ctgtttcccg
    14461 ccttcgtcaa ccaaacgccc gctttcgatg aaaagggtca aatgccagtt gccgtctatg
    14521 cctttcaccc ggccgatgcg gtcgccgcgc ccggtaaact tgaacggccg tatgggtttg
    14581 aacggcatac ccatacggcg ttcgacttcc gtgcggaagt tgtccaagcc catattttga
    14641 atggtgtagc gggtgcggtc gtttttgcgg tggctgcggt tgccgaagtt gcgctgcgtg
    14701 gttaccaccg cttcggcggc cttcagtgcg tgttccggag gcacgaaacc cagttccagt
    14761 gaaatgttcg gataggtttt ggtgttgccg ttgccgtgtt ccgtcgaaag cccgccgccc
    14821 gccaaaacat tgaagccggc aagctgtccg ttgccgtctg aaacggcgac gaaatccaaa
    14881 tcgttaccgt agcagtccac atcgttcaag ggcgggatga cgactgcggt tttgaatttt
    14941 cgcggcagat aggttttgcc caaaatcggc tcgccttctt gaaggaagtc gtcggaactt
    15001 tgaacttttt tgccgtccac ccacatatcc agataaccgc gcgtgcgcgg cagcaggtgt
    15061 tcggaaatct ttttcgcgta ttcgtaagcc tgccggtgca gctcggactc gatcgggttg
    15121 gaggtgcaaa gcacattgcg gttcatatcc gccgccgtag cgatggaatc caaacccagg
    15181 ctgtgcaaga ggcggggcat cgtctgcaac ttggttttcg gcacgccgtg aaattggaag
    15241 gtttgccggt tggttagccg gatggagcgg taatgactct tttcccgggc gaatttgtcc
    15301 agttctatcc actgggacgg tttgatgatc ccgccggcag ccggcagcgc aaaagcataa
    15361 atttcaaggg ctcgagtttt gcctcggtgc ggatgtcgcg gccatcctgc tcatacatac
    15421 tgtggaagcg gatgagttgg aagttgtcgc ctttgaagcc gcccgtgagc gggtctttca
    15481 aatcgtccaa aatcgtgccg cgtaaaaaat tgctttcggt tttcaggcgt tcgttgtcgg
    15541 atagcggctt ttcttgccac gccaaacctt ttgtcttggc ctgtacggtc attttgtgtt
    15601 cctcccgatt atatttaatc aataaacatc acgctgatag cgtttttctt cgcgcagcat
    15661 atccaaatat ccttctgcgc cgtcttcgtc cgaatgccct gccccgataa tcacatccag
    15721 caaggcggct tccacttctt ttgccatttt tgccgcatcg ccgcacacat agatatgcgc
    15781 gccttcctgc agccattgcc aaagtccttc cgcctgttcg cggattttgt cctgcacata
    15841 gattttttct tcctgatcgc gcgaccaggc gaagtcatat ctgtgcagga agccgtcttt
    15901 ggcaaactgc tgccattcgg tctgatagag gaagtcggcg gcaaaatgcg gattgccgaa
    15961 aatcagccag tttctgcctt ccgcattttc tgcggcacgt tgttggacga aggcgcggaa
    16021 cggtgcgacg ccggtaccgg agccgatcat cacaatcggc ttgcggctgt cttcgggcag
    16081 cctgaagccg tcgttgcgtt ccgcaaacac gcgcaccgtg ccgtcctctt ccagccggtc
    16141 ggcaaagaaa cccgatgcgc cgcccgccct ggcgcgccct tcgtgttcga aacgcactgc
    16201 gccgacggtc aggtgcactt cgtcccccgc ttccgcctgc gacgaggaaa tcgaatacag
    16261 gcgcggcgca agcgggcgca gcaggccggc gaattgttcc gccgtcagtt ttgccgggaa
    16321 gcggtgcagc acaccggcaa tcggcgtgct ttgcacaaag ccttgcaaaa cggcgttgtc
    16381 ggcagcaata cggtcgagtt cgtcattatc ggcgaacgtg gcatagcctt tgacaaaggc
    16441 gggggtgttt tgcgtgagtt cgaaatggga taacagtgcg gaggcaaccg gcagggtttt
    16501 tccgcccgcc tgtatttccg ttgccggatt gatgccgagc aggtctagga tttccccgac
    16561 cagtgccgga tcgttgtcaa accaaacgcc gagcgcgtcg cccgggaggt agtgcaaatc
    16621 cgaaccgctc aaatcgattt cgatgtggcg cacgtcttta tcggattggc gggcggtgat
    16681 tttctgattg gccagcaggg cggcgggaaa ggggtctgcc ttgcagtacc tgccctccga
    16741 tgccgtctga aggccggcgg ggggacgttg cctgcggcgc gggcgttgcc cggttttttg
    16801 cggcttcttc ttttaagcgc gcgacaatgc ggcctgtcca tccgtctgcg gcggcggcaa
    16861 agtccaaatc cgcatcaacg cgttcgagca ggcgttttgc gcccaattct tcaaaacgtt
    16921 tgtcgaaatc tttgcccgcc cggcagaaat tcggatagga gctgtcgccc aaacccagta
    16981 cggcaaattg gagtttgtcc aatttcgggg cttttttgcc gttcagcagt ttgtgcagca
    17041 cgacggcttc ttccggcggt tcgccttcgc cctgggtgga cgtaaccagc agcaggcggc
    17101 gttcgccggc gatgtttttc gccttgtagc ctttcagttc ggcgcgcctg acttggatgc
    17161 cggcggcttt caggctgtcc gccgctttgt cggcaacgga tttcgcattg ccggtttgcg
    17221 aggcggaaag gacggttacg gaaaaaggtt ctgccgccgg caatgccgtc tgaagcgcgg
    17281 gcagccctgc ggatgccccg tttcctgctt ttgcccaagc gtagccggac agccccgaca
    17341 caggagctgc gtgatttcgg gcggcatagg cggtaatggc ggatttgtgt tctgcatatc
    17401 gtgttcactc ataaaatcat acctgccgca acagtaccgt atgtcgcttc gtctatcagg
    17461 ataaacgaac cggcggcggt gttttccgca taaggcgttg ccgtaacggg tttttgaagg
    17521 ttgatgcgga ctttggcgat gtcgttcatc tttaaggatt ccgcgccggc ctcttgttcc
    17581 agcgtgcgga catccaaaac gctttcaatt tccccgactt ttgccggcac ggtttgcgtg
    17641 ccgtgcttga gcaggtattt gcgcgcggtg ttgagcgggc gttcgtcaaa ccagcaaagc
    17701 gtggcttccg gatgtttttg cggggcgagc gggaaatttt tatcgacaaa aagatcgccg
    17761 cgcgaaacat cgatgtcgcg gtccagccgg atggttgccg cctcgccggc aaaagcctgc
    17821 gccacttccc ctttcggcgc gatgatttcg gacacttcgg cggtcagccc gttcggttcg
    17881 atgcggacgg tttgcccgac ggtgaccgaa ccgcgttcga tgcgcccctg atagcctcgg
    17941 aaaccgtcgg ccttgtcggc atcttggcgg acgaccagtt ggacggggaa ataaaaaccg
    18001 gcatcgcgcc accttcctga aatttccgcc atatcgccca aagacggcgg cagcaaggct
    18061 tccagccttt cacgcagcag gcgggcgagg acgggcgcgc tgccggagct ggaaacggca
    18121 atctgaacag ggtcgcggtc gataaccgac gggaagatga agctgcaatg gtcacggtcg
    18181 cccaccacgt tgaccggctt ttggcagctt tcggcaagat ggaaaacgcg ccggttgagg
    18241 gcttggtcgc tgcttgccgc aatgatgagg aaaaccgtgc ggatgtgttc ggcacgaaat
    18301 tctttggcaa gccacaggat tttgttttcc gccgccaacg cggagagttc ggcattcagg
    18361 tgttttgcgg caaccccgac ctctgcgccc gccttcggca gcaggctgat tttgcgtgcg
    18421 gcgaccgcgc cgccgcctac gaccaatacg gggcggccgg agaggttggc gaaaatggga
    18481 aaataattca ctggctgact cctttgctgt ttgcccgcac cttgtttccg atacggtgcg
    18541 tcgcggcatt tttgtcggaa tgcgggtcat tttaaataat aaaaaggcat ttgttagaag
    18601 ctgaaagcta tatggggggc ggctgcggat gcggcggttt tc
//
LOCUS       DAXDXW010000041        18623 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXDXW010000041 DAXDXW010000000
VERSION     DAXDXW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18623
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.042"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            152..712
                     /locus_tag="ACFB64_001216"
     CDS             152..712
                     /locus_tag="ACFB64_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC1209577.1"
                     /translation="MKKIIFAALAAAAVGTASATYKVDEYHANVRFAIDHFNTSTNVG
                     GFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQPFTGHLKSADIFDAAQYPDIRFVS
                     TKFNFNGKKLVSVDGNLTMRGKTAPVKLKAEKFNCYQSPMAETEVCGGDFSTTIDRTK
                     WGVDYLVNAGMTKNVRIDIQIEAAKQ"
     gene            776..913
                     /locus_tag="ACFB64_001217"
     CDS             776..913
                     /locus_tag="ACFB64_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209578.1"
                     /translation="MAEHNPPKDILAAGGACRKVRKVTTENNVSRFQTAYDMDNFIRP
                     V"
     gene            989..2386
                     /gene="aspA"
                     /locus_tag="ACFB64_001218"
     CDS             989..2386
                     /gene="aspA"
                     /locus_tag="ACFB64_001218"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFC1209579.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEAFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAESGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHRNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(2454..3944)
                     /locus_tag="ACFB64_001219"
     CDS             complement(2454..3944)
                     /locus_tag="ACFB64_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC1209580.1"
                     /translation="MNKPLSLALFCLLLPITAHADNPADERRRLLDEGSRQTQQYRES
                     GWLDTEQARGEVEENDGYISIGGEIYQVGDTAEELESAIYHALNARQWHKVRQFAARY
                     AKLPRHKPALIHLADALQKRDEGDFRAAGNSFQTALEAEPDNPRLLLEAGRFYAEDNQ
                     NKESAAAFEKVLKTDIPAETRPIVENYLSELGKRRRWHGQISLGYGYNSNVNQGNGIN
                     QCVWEIAGMCLMERTLPAPTDSTFSSYSATAEKTVPLKGHHGVQVRGVLYGNRYTEKD
                     KDSAAMPDYGYRNGSLYAGYAYADARSSFSLLPYFEYDFRNRHTHYRAWGADADWSRT
                     LSPHWRINSHAGAKKTGYGGQSKTYFADFKQYELGAGAEFSITPKSGLLVNFDAARKA
                     YPEKSSSSKEYTARLGAYRLFSGGTYLNAVLLYRRSLYDAASFVSDNKRRSDKQYIMM
                     AAAGFPQWNIKGVYPELRFRRTIAHSNAVYYRYRQNEWLLGFKYRF"
     gene            complement(4215..5198)
                     /locus_tag="ACFB64_001220"
     CDS             complement(4215..5198)
                     /locus_tag="ACFB64_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slam-dependent surface lipoprotein"
                     /protein_id="HFC1209581.1"
                     /translation="MSIRNKTLLAVLLCGVFGVAAAAPAGGSSQRKSQDPNSDPTTVR
                     PGTSDKQPFGAGKAGLAFSINNGGSWSKSINLDDGPVKYIRAKYQAPINGNDPYANVD
                     GTRNLQYLRLGDVKPVIGWFYNPTLGQVWYENHHNNTEVYSVRQIANPAIPAAPKFGG
                     LVIAKVPGMGGSNGNVFFGEWAPRAGNPQQISTDLNMADGKRTVWYVGDNPTGNTTGV
                     RTATYNVLGINKHTPGQNDFYTGQVTATFGSGITGTMAGRISRSSDHVDFDHAKIDNR
                     NGTFADPSQGMTGRFYGNQAAAMAGYTTRGTGNVGDDVAFGGSATDIETRK"
     gene            complement(5413..8841)
                     /locus_tag="ACFB64_001221"
     CDS             complement(5413..8841)
                     /locus_tag="ACFB64_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFC1209582.1"
                     /translation="MMSGFSPKPKTIILSLAGAFGALAFADTPNNTEQQKELNTIVVH
                     GKRSADQKGADDVYYKNVSNAYVGKEYLERYRVQSAGDVLKGLNGVYNMNTRTAGGAI
                     TPNIRGITGKGRIPVTIDGTEQTIDVWMNNYGVGDRNYLDPALFRSIAVEKSPALTRG
                     VKSGVGGAMSIRTIEAGDIVPEGQKFGFQLKTEFANNRTRPANNLNQWLGWEDYRTLP
                     LGATADGAGGGSDPITGQQSPQALVVDNFTPPQHKSGRDNWRFGGDRSYMAAAAFKTE
                     LSDGLAAYSYRNKGNYFAGRKGAEGYLNNPVYDLQKCYDEGGSDFNCKNSATFIPNMA
                     RIYHPGVEVLNSNTETKTLLLKNNWHLPGSHRLGWQYMRTDVRFGEINPFHTTYVMNM
                     EEHNETNRPKELSPQAQSIDSTIRTDTYKLGWAWKPENSRWVDLQANLWRIKTNSTRH
                     QSGGMDLSSARPDPFYDVWYWCTQRGRIPPEHVDNYSSCNDLMNDFGVNGLTKEQVLA
                     MTPNDNGQFRVISGAEQKTRVSRTGFDISNRFRLRDRLSMTLAADYQKEKLAEEVEIV
                     NSQDLFNLAGMVTGLTKLAGPRGGERREWGTNLVFDWQAADRLKISAGIRYHNFRGFD
                     TALAEGRARRDPRYQAGDGTQHYADGAYLPYFELAGDQERRDWNVVAEQLRQAYQSGD
                     AAAIAAAVQAEQAHGARYHLLKYYDGSYRTDGYVYIGNDGRAYFDGRFGGVNEAQNQP
                     LYRVRPVYVPFVNGKLDSGALPQHFHDFVSNYQEKVTNPQGLHGTHYRYWAGVGNPID
                     CIRSDAASCLAHNLSGKVTNFQVGYPEYGDDTGLGLKIIGRHYTEEQYWAQPKPIRAH
                     AWAPTIAVSYDLTDNSRLFVRYAQMSRFPSVYEVGSFYNDVAYAGMPKAPNFRFKPER
                     SRSWEIGYSFNFAPYWSRLRAGDMRLTYYRNRIQNVIETTDYFRTVQYDRKDTAGLEW
                     QSRIDTGRFFAALGATYRLKQQMCDRDTAFDYDPYGAKGVPICIEGGYGSTRGYQALQ
                     PKYSINLDAGVRLLGEKLELGLRGIYHSRVNTKQYDQLLQKDLGIIFDTTGKPHHWRS
                     SLTWDVYGRYRLHKNLNVNLGITNLTNRYYLDPMSNVAAPGPGRTVTFGLTAKF"
     gene            complement(9049..9924)
                     /locus_tag="ACFB64_001222"
     CDS             complement(9049..9924)
                     /locus_tag="ACFB64_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC1209583.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     SVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKSDSGKKSGSAWRQALLLLGVWAGYGI
                     IDILFKQIAKSGTAFAGNLLVAFALAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDSPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLSLCSI
                     ACLFYWTEVKALFGI"
     gene            complement(9928..10620)
                     /locus_tag="ACFB64_001223"
     CDS             complement(9928..10620)
                     /locus_tag="ACFB64_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFC1209584.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPQAEE
                     KFKEIQRAYDTLSDLSKRTQYDASFRRHEERGRQEEAFRREQARREQFYGEQMRREQA
                     FRREFERQASRSRHAYEPSGSGSGRNYILAAYILFGLGAIMQFMPIVGVILAYMKRNS
                     LDSIVYAAHTEYLIKTFWRTFWLYLLGALTARFGIGVLVIIATTVWYFYRIIAGFIRF
                     NDGRAVAPEKWI"
     gene            complement(10642..11121)
                     /gene="ybaK"
                     /locus_tag="ACFB64_001224"
     CDS             complement(10642..11121)
                     /gene="ybaK"
                     /locus_tag="ACFB64_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC1209585.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGPIVLMHGDKQISTRNLARHLGAKHIEPAAPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPDDLNILNPKTIQAAV"
     gene            11196..11558
                     /locus_tag="ACFB64_001225"
     CDS             11196..11558
                     /locus_tag="ACFB64_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC1209586.1"
                     /translation="MDIRYFGTTPRYSEAVCAGGLIFLSGMVPENGETAAEQTADVLA
                     QTDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            11593..12456
                     /locus_tag="ACFB64_001226"
     CDS             11593..12456
                     /locus_tag="ACFB64_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC1209587.1"
                     /translation="MKTLLPFFGVARVSGEDRQTFLHGQLSNDINNLQAGQACYATYN
                     TPKGRVIANMIVVNRGDDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     AASAEPLAAQEPSLAFTSECVSDGICSVILHHRGILHIAPETALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEAGILLTADGEEAGIVLDSVQDSENFTALAVIKFSAAQKELTVPNGGIFKA
                     VHLFFKTKNAK"
     gene            12548..13507
                     /gene="ttcA"
                     /locus_tag="ACFB64_001227"
     CDS             12548..13507
                     /gene="ttcA"
                     /locus_tag="ACFB64_001227"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC1209588.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDTINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATMFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQNLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(13601..16540)
                     /locus_tag="ACFB64_001228"
     CDS             complement(13601..16540)
                     /locus_tag="ACFB64_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFC1209589.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGICTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIQMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFGEIF
                     YDSDIEHENVANNQIEGVIVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENEH"
     gene            complement(16630..>18623)
                     /locus_tag="ACFB64_001229"
     CDS             complement(16630..>18623)
                     /locus_tag="ACFB64_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC1209590.1"
                     /translation="PDLSDTAGANTEAIYTSDGITANSTQLEQLKKLFPACFDADGNF
                     LIDRLQAEIAPQTDIGREFYEMNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQ
                     NLLIRGDNLEVLKHLKNAYANSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELALLANI
                     DEDEAARILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLL
                     CDEVFGEGNFVAQLPWRKRTAKSDVPFGISQDYEWIFVFAKSCQFIAATKGKERRYYE
                     TDDFPDRPWRTHDLTKQTTAAERPNSFFTMVDPKTGKKYPANPNATWRVTKDTFQDYY
                     NKGKIVFPDDYDFLNISNPVMRYFKDDDMKKAGEDFGKVAVSSRLPENVGTLADAVAE
                     YLAIFSRTLPENIGMTKEGTKEITDLFGSKIFTFPKPSQLIKFLVSISSKSNDLILDF
                     FAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARKAGYPTIFDITKARIEK
                     VAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDLPLNDELSEEQLQTLLT
                     TWTLYDGAALTTPVEPVRLGSYTAYLCEKRLYLLNKGFTSADLLAFIKKLDDDADFNP
                     NRVIVFGSNMASAMQHELDQAVRGYTNKKEIELNVIIRV"
BASE COUNT         4151 a         5010 c         4833 g         4629 t
ORIGIN      
        1 cacggataac accgcgcttt ttcatccgcc ctgttccgcc cgcccgttcg tcaaccgcct
       61 gcaaacccac cgtttaccgg caggaaatgc cataaatccc cgcctatgtt aggataaacg
      121 cctttaccgg cccacacaaa ggattatcat catgaaaaaa atcatcttcg ccgcgctcgc
      181 agcggcagcc gtcggcactg cctccgccac ctacaaagtg gacgaatatc acgccaacgt
      241 ccgtttcgcc atcgaccact tcaacaccag caccaacgtc ggcggttttt acggtctgac
      301 cggttccgtc gagttcgatc aagcaaaacg cgacggcaaa atcgacatca ccattcccgt
      361 cgccaacctg caaagcggtt cgcaaccctt caccggccac ctgaaatccg ccgacatctt
      421 cgatgccgct caatatccgg acatccgctt cgtttccacc aaattcaact tcaacggcaa
      481 aaaacttgtt tccgttgacg gcaacctgac catgcgcggc aaaaccgccc ccgtcaaact
      541 caaagccgaa aaattcaact gctaccaaag cccgatggcg gaaaccgaag tttgcggcgg
      601 cgacttcagc accaccatcg accgcaccaa atggggcgtg gactacctcg ttaacgccgg
      661 tatgaccaaa aacgtccgca tcgacatcca aatcgaagct gcaaaacaat aagccttgat
      721 gcgaatcagc acaaatgccg tcttcatcaa accgccctgc caaccgtaag ccgatatggc
      781 ggagcacaac ccgccgaaag atattttggc ggcaggcggg gcttgccgga aagtccggaa
      841 agtaacgacc gaaaacaacg tttcccgttt tcagacggca tacgatatgg acaatttcat
      901 ccggccggta tagtgttcgt cctgctgcgg catctttccg atggcgcatt ttttcaatat
      961 taattatccg acttacaaag gtaaaaacat gactgtccgt atcgaacacg atttattggg
     1021 cgaccgcgag attcccgccg aagtgtattg gggcatccac accctgcgcg ccattgaaaa
     1081 ctttaaaatc tccacacaaa aaatttccga cgtgccgcag tttgtccgca gtatggtgat
     1141 ggtgaaaaaa gcaaccgcgc aggcaaacgg cgaattgggt gcagtaaaac ccgaaatcgc
     1201 cgccgccatt gaaaaggctt gcgacgaagt tctgctgaac aaccgctgcc tcgaccaatt
     1261 cccgtccgac gtgtatcagg gcggggcggg aacttcggtc aatatgaaca ccaacgaagt
     1321 catcgccaac cttgcattgg aagccttggg ctatgagaaa ggccgctacg acatcgtcaa
     1381 tccgatggat cacgtcaacg ccagccaatc caccaacgat gcctatccca cgggcttccg
     1441 ccttgccgtg tattacagca tcggcgaatt gctcgacaaa ctgaccgtat tgaaaaacgc
     1501 ctttgccgcc aaagccgaag cgtttaaaga cgttttgaaa atgggtcgca cccagcttca
     1561 agatgccgta ccgatgacgg caggccagga attccaatct ttccaagtat tgttggaaga
     1621 ggaaatcctc aaccttgacc gcacccgcca actgctcttg gaagtcaatt tgggcgcaac
     1681 ggcaatcggc acgggcgtga acacgcccaa aggctacgcc gaactggtgg tcaaaaaact
     1741 ctccgaagtc agcggcttgc cttgcaaact gactgaaaac ctgatcgaag cgacctccga
     1801 ctgcggtgca tatgtgatgg tacacggcgc attgaaacgc acggccgtca aactctctaa
     1861 aatctgcaac gatttgcgcc ttctctcttc cggtccgcgc gccggtttga aagaaatcaa
     1921 cctgcccgaa ttgcaggccg gttcttccat catgcctgcc aaagtcaatc ccgtgattcc
     1981 tgaagtcgtc aaccaagtct gcttcaaagt catcggcaac gatacgacga ttaccttcgc
     2041 cgccgaatcc gggcaactgc aattaaacgt tatggagccg gtcatcgccc aatgtatgtt
     2101 tgaaaccatt tccctcttgg gcaatgccgc agtcaaccta tccgacaaat gcgtcaaagg
     2161 cattacggtc aaccgcgaaa tctgcgagcg ttacgttttc aactccatcg ggctggtgac
     2221 ttatctgaat ccgtacatcg gccaccgcaa cggcgacttg gtcggcaaaa tctgcgccca
     2281 gaccggcaaa ggcgtgcgcg aagtcgtact ggaacgcggg ttgttgagcg aagaagaact
     2341 caaccgcatc ctctcccccg agaacctgat gaatcctcat ctgtaaacat tacggacaaa
     2401 tgccgtctga aaggaagcgc cctttcagac ggcatttttt atcacccgac tggtcaaaac
     2461 cgatatttaa aacccaacag ccattcgttt tggcggtagc ggtaatacac cgcgttgctg
     2521 tgtgcgattg ttcgcctgaa acgcagttcg ggatatacgc ctttgatatt ccattgcgga
     2581 aaacccgccg cagccatcat gatgtattgc ttgtcgctcc gccgtttgtt atcgctgacg
     2641 aaacttgccg cgtcatacag gctgcggcgg tagagcagga cggcgtttaa ataagtaccg
     2701 cccgaaaaaa gcctatacgc gcccagccga gccgtatatt ctttgctgga agaggatttt
     2761 tcgggatagg ctttgcgtgc ggcatcgaaa tttacaagca ggccgctttt cggcgtgatg
     2821 gaaaattccg cccccgcgcc gagttcgtat tgtttgaaat cggcaaaata agttttgctt
     2881 tgtccgccgt atccggtctt ttttgctccg gcatgtgagt taatgcgcca gtgcggggag
     2941 agtgtgcgcg accagtcggc atccgcaccc catgcgcggt agtgggtgtg gcggttgcgg
     3001 aagtcgtatt cgaaataagg cagcagggaa aaggaggagc gtgcgtcggc ataggcgtat
     3061 cccgcgtaca gcgaaccgtt gcgatagccg taatccggca tggccgccga atctttgtct
     3121 ttttccgtgt agcggttgcc gtacagtaca ccgcgcacct gtacgccgtg atgccctttg
     3181 agcgggacgg ttttttcggc ggtcgcgcta taggatgaga aggtcgaatc cgttggcgcg
     3241 ggcagggtgc gctccatcag gcacatgccc gcaatttccc acacgcattg gttgatgccg
     3301 ttgccttggt ttacattgct gttatagccg tagccgaggc tgatttggcc gtgccagcgg
     3361 cggcgtttgc ccagttcgga caggtagttt tccacgatgg ggcgggtttc tgcgggaatg
     3421 tccgttttca gaactttctc aaacgcggcg gcagactctt tgttttggtt gtcttcggca
     3481 taaaaccgcc ccgcttcgag cagcaggcgc gggttgtcgg gttcggcttc caaggccgtc
     3541 tgaaaactgt tccccgccgc gcggaaatcc ccttcgtccc gtttttgcag cgcatcggcc
     3601 aaatgaatca gcgcgggctt gtgccggggc agtttggcgt agcgggcggc gaactgccgc
     3661 accttgtgcc actgccgcgc attgagggcg tggtaaattg cgctttccag ttcttccgcc
     3721 gtatcgccga cttggtagat ttcgccgcct atcgaaatat agccgtcgtt ttcctctact
     3781 tccccgcgtg cctgttccgt gtccagccaa ccgctttcgc ggtattgctg cgtttggcgg
     3841 ctgccctcgt ccagcaggcg gcggcgttcg tcggcggggt tgtcggcgtg ggcggtgatg
     3901 ggcagaagca ggcagaagag ggcgaggctg agtggtttgt tcatggttgc cttccgaaaa
     3961 gataaagtaa cgcaggtttt aattgctgtc ccaaaatgtc cgataggccg aaaccgtatg
     4021 ggataggcga tgtttattta caaatatcaa atttcaggta gcctgtgttg aacacgggct
     4081 acctgaaacc gagcagcaat ccgtatcgga cggatgattt atatggcttg gcagtcggca
     4141 taaaacggca gcttgaaact cctcaggggt taaatcgagc ctagccgttg ccaaaccaaa
     4201 tcggatggca gctgttattt gcgggtctca atatcggtgg cgctaccgcc gaaagctacg
     4261 tcatcgccga cattaccggt accacgtgtg gtatagccgg ccatggctgc cgcctggttg
     4321 ccgtagaagc ggccggtcat gccttgactg ggatcggcaa aggtaccatt tctgttatcg
     4381 attttggcgt gatcaaaatc gacgtgatcg gaactgcggc tgattctgcc tgccatcgtc
     4441 ccggttatac cagaaccgaa ggtggcggtt acctgaccgg tatagaaatc gttctgcccc
     4501 ggggtgtgct tattgatacc cagcacattg taggtggcgg ttctcacacc ggtggtgttg
     4561 ccggtgggat tatcgcccac ataccaaacg gtgcgtttac catccgccat attcaggtcg
     4621 gtgctgattt gctgcgggtt accggcacgc ggcgcccatt cgccgaagaa gacgttgccg
     4681 ttgctgccgc ccatgcccgg cactttggcg attaccaggc cgccgaactt gggtgcggcg
     4741 gggattgccg ggttggctat ctggcgcaca ctgtacactt cagtgttgtt gtggtggttt
     4801 tcataccaca cctgacccaa cgttgggttg tagaaccagc cgataaccgg tttcacatcg
     4861 cctaagcgca gatattgcag attgcgcgta ccatcaacat tagcataggg atcgttgccg
     4921 ttaatcggtg cctgatattt cgcacggata tatttgaccg gaccatcatc cagattgatg
     4981 gatttcgacc aacttccacc gttattgata ctgaatgcca aaccggcctt accggcaccg
     5041 aaaggttgct tgtcagaggt gccggggcga actgtcgtcg ggtcactatt tggatcttgg
     5101 cttttccttt ggctggaacc accagccgga gcagccgcag ccacaccaaa aacgccgcac
     5161 agcaagacgg ccaacagggt tttattacgt atagacataa tcgtttcctt aattaagagt
     5221 taaacaaaag ccaccgtttt attaccgccg aaccggcgga aaaaacggta aaaaccatta
     5281 cctcggataa gcggggccgg ttgggtttgt gcgtccggag tgttcaattg tttgttgtta
     5341 tatccaatcc gttttcaggt agccttcccg ccatacccgc aggctacctg aaaaccccac
     5401 tccgccctgc ggctagaact tggcagtcag cccaaaggta acggtccggc ccggcccggg
     5461 ggcggcaacg ttggacatcg ggtcgagata gtagcggttg gtcaggttgg tgatgccgag
     5521 gttgacgttg aggtttttgt gcagccggta gcggccataa acatcccaag tcagcgatga
     5581 ccgccaatgg tggggcttgc cggtggtatc gaagatgatg cccaaatcct tttgcagcag
     5641 ttggtcgtat tgcttggtgt tgacccggct gtggtagatg ccgcgcaggc cgagctccag
     5701 tttctcgccc aacaggcgca ccccggcatc caggtttatt gaatatttcg gctgcaaggc
     5761 ctgatagccg cgcgtactgc cgtagccgcc ttcgatgcag atcggcacgc ctttggcacc
     5821 ataagggtcg taatcgaaag cggtatcgcg gtcacacatc tgctgtttca ggcggtaggt
     5881 ggcaccgagc gcggcaaaga agcggccggt gtcgatgcgg ctctgccatt ccagcccggc
     5941 ggtgtccttg cggtcgtatt gcacggtgcg gaaataatct gtggtttcga tcacattttg
     6001 gatacggttg cggtagtagg tcaggcgcat atcgccggca cgcagccggc tccaatacgg
     6061 ggcaaaattg aagctatagc cgatttccca gctgcggctg cgttccggct tgaagcggaa
     6121 atttggggcc ttcggcatac cggcataggc cacgtcgtta taaaaactgc ccacctcata
     6181 cacgctgggg aagcggctca tctgcgcata gcgtacgaac aggcggctgt tgtcggtcag
     6241 atcgtagctg acggcgatgg tcggcgccca ggcatgggca cggatcggtt tgggctgcgc
     6301 ccaatactgt tcctcggtat agtgccgacc gatgattttg agaccgagac cggtatcatc
     6361 accgtactcg ggatagccga cttgaaaatt ggttactttt cccgataggt tatgagcaag
     6421 gcaagaggcc gcatcacttc ttatacagtc gataggattc cccacgcctg cccaatatcg
     6481 ataatgcgtc ccgtgcagcc cctgcggatt ggtcactttt tcctgataat tgctgacgaa
     6541 atcatgaaaa tgctggggca atgcgccgct gtccagcttg ccgttgacaa acggcacata
     6601 aacggggcgc acccgataga gcggttggtt ctgtgcctcg ttaaccccgc cgaaccgtcc
     6661 gtcaaaatag gcacggccat cgttgccgat atacacataa ccatcggtac gatacgagcc
     6721 gtcatagtat ttcagcaaat ggtaacgggc gccgtgcgcc tgttctgcct gtactgccgc
     6781 ggcaatggcg gcagcatcgc ctgactgata cgcctgccga agctgttcgg ccaccacatt
     6841 ccagtcacgc cgctcctgat cgcctgccag ttcgaaatac ggcagatagg caccatcggc
     6901 ataatgctgg gttccatcgc ctgcctgata gcgcgggtcg cggcgggcac gcccttcggc
     6961 cagggcggta tcgaagcccc ggaagttgtg gtagcggatg ccggcggaga ttttcaggcg
     7021 gtcggcggcc tgccagtcga acaccaaatt ggtgccccat tcgcgccgct cgccgcccct
     7081 gggcccggcc agcttggtca ggccggttac catgccggcc aggttgaaca ggtcttgcga
     7141 attgacgatt tccacttcct ccgccagttt ctctttctga taatcggcgg ccaaggtcat
     7201 gctcaggcgg tctctgaggc ggaagcggtt gctgatatcg aaaccggtgc ggctgacgcg
     7261 ggttttctgc tcggcgccgg aaatcacgcg gaattggccg ttgtcgttcg gggtcatggc
     7321 caacacctgt tctttggtca ggccgttgac gccgaaatca ttcatcaggt cgttgcagct
     7381 gctgtagttg tcgacgtgct cgggcggtat ccggccgcgt tgggtacacc aataccaaac
     7441 gtcgtagaag ggatcggggc gggcggaaga caaatccatg ccgccactct ggtggcgggt
     7501 gctgttggtt ttgatgcgcc acaggttggc ctgcagatcg acccagcggc tgttttccgg
     7561 cttccaggcc cagccgagtt tgtaggtgtc ggtgcggatg gtggaatcga tgctctgcgc
     7621 ctgtggcgac agttctttgg ggcggttggt ctcgttgtgc tcttccatat tcatcacata
     7681 ggtggtgtgg aaggggttga tctctccgaa gcgcacgtcg gtgcgcatat attgccagcc
     7741 gagccgatgg ctgccgggca ggtgccagtt gtttttcagc agcagggttt tggtttcggt
     7801 gttgctgttg agcacttcca ccccggggtg gtagatgcgc gccatattgg ggatgaaggt
     7861 ggcggaattt ttgcagttga aatcgctgcc gccttcgtcg tagcattttt gcaggtcgta
     7921 taccggatta ttcaggtagc cttccgcacc cttgcgaccg gcgaagtagt tgcctttgtt
     7981 gcggtagctg taggcggcaa ggccgtctga aagttcggtt ttgaaggcgg cggcggccat
     8041 ataactgcgg tcgccgccga agcgccagtt gtcgcggccg cttttgtgct gcggcggggt
     8101 aaagttgtct accaccaacg cctgcgggct ctgctgtccg gtgatcgggt cagaaccgcc
     8161 gcccgcgccg tcggcggtgg cgccgagcgg cagggtgcgg tagtcttccc agcccagcca
     8221 ttggttgagg ttgttggccg ggcgggttct gttgttggca aactcggttt tcagctggaa
     8281 gccgaatttc tgcccttcgg gcacgatgtc gccggcttcg atggtgcgga tggacatcgc
     8341 gccgccgacg cccgatttca cgccgcgcgt gagggcgggg cttttttcca ccgcgatgct
     8401 gcggaacagg gcggggtcga ggtagttgcg gtcgcccacg ccgtagttgt tcatccatac
     8461 gtcgatggtt tgttcggtgc cgtctatggt aaccgggatg cggcccttgc cggtgatacc
     8521 gcgtatgttg ggcgtgatgg cgccgccggc ggtgcgggtg ttcatgttgt acacgccgtt
     8581 taggcctttg agtacgtcgc cggcggattg gacgcggtag cgttcgaggt attctttgcc
     8641 gacgtaggcg ttggagacgt ttttgtaata cacgtcgtcc gcgccttttt ggtcggcact
     8701 gcgtttgcca tggacgacta tggtattcag ttctttctgc tgttcggtat tgttcggggt
     8761 gtctgcaaaa gccaatgcgc caaatgcacc tgcaaggctt aggataatgg tcttaggttt
     8821 gggggagaat ccggacatca tgtattcctt attaagaata taaaatgaat tttattaaga
     8881 tacactcata ataagaatta tttgaaattt ttaattattg ttaatattta aattataatt
     8941 aaaaataact attattaatt aaattttgaa tctgcccgta taacaaaatg ccgtctgaag
     9001 cagcattttc ctgtttcaga cggcattttg ttatatcaat cagccggatt atatgccgaa
     9061 cagtgcctta acttctgtcc aatagaacag gcaggcaatg gagcataatg acagtacaat
     9121 gcctgccgta ttgattgtgt tgattttttc cttaaagaac aatgcgcccg aaagcgtccc
     9181 taaaacaatc acaccgatat tcataccggc aaaaaccagc gtgggactgt ccttcatcat
     9241 ttggtgcgca gtgatgtagg ttacgatgtt cataaaattc aaaccgccca agagtatgcc
     9301 gccgaccacg ctctcaacgc gccatctgac cgatttggca aacaggcagg caaacatcag
     9361 cacacccgcc agcgcaaatg caaccagcag gttgcccgca aatgccgtgc cgcttttggc
     9421 aatctgtttg aacaggatgt cgataatgcc gtaacccgcc cacacaccga gcagcaataa
     9481 cgcctgccgc cacgcgctgc ctgatttttt gccgctgtcg cttttccaaa gcaggcagaa
     9541 cagtgcggca aaggcgaggc acagtccgat cagtttgcct tcactgagtt tttcgccaaa
     9601 cagcgtcaaa gcggcaacaa tcggtaaaaa cagcgacaaa cgctgcgccg cgtcggattt
     9661 gacgataccg gcggattcta cggatttgcc cataatgaca aatacggacg gcagcagcac
     9721 gcccaaagcg gcaaacagcg gccacgtcgg caaaaatgca ccgatattgc cgacatccgg
     9781 cttcaatacc gacagggtca gtatgaccgc gaccacataa ttgacggcga ccgcctgcgc
     9841 gatgtcgatt tttttcttcc ttgccatttt cagcaaaatg gaaaccgaca cgctgaacac
     9901 gatgctgatt aacaggtagg ccatgctcta tatccatttc tcgggcgcaa ccgccctgcc
     9961 gtcgttgaag cggataaagc cggcgatgat gcggtagaaa taccagacgg tcgttgcaat
    10021 aataaccagc acgccgatgc cgaaacgggc agtcagcgcg cccaaaagat aaagccaaaa
    10081 tgtgcgccaa aaggttttaa tcaggtattc ggtatgtgcg gcatagacaa tgctgtccaa
    10141 actgttccgt ttcatatagg cgagaatcac gccgactatg ggcatgaact gcatgattgc
    10201 acccaaaccg aacaggatgt aggcggcgag gatatagttg cgcccgcttc cgctgccgga
    10261 cggttcgtaa gcatggcgcg aacgtgaagc ctgccgttca aactcccgtc tgaacgcctg
    10321 ttcgcggcgc atctgttcgc cgtaaaactg ctccctgcgc gcctgttcgc ggcggaatgc
    10381 ctcttcctgc cgcccgcgtt cctcatgccg tctgaaggac gcgtcgtatt gcgtccgttt
    10441 tgacaggtcg gaaagcgtat cgtaagcccg ttggatttct ttgaactttt cttccgcctg
    10501 cggattgccc ggattgcggt cgggatggta tttcatcgcc agcttgcggt aggcgcgttt
    10561 gatttcgtcc gcccccgcct gcggcgacac gcccaatacg gcatacaaat ctttgtccat
    10621 attgctcctt atacttcccg gtcaaaccgc tgcctgtatt gttttcgggt tcaaaatatt
    10681 taaatcatcg gggcggatac cgataatgaa cccgcgtttt ccgccattga tatagatggt
    10741 ttccaaatcc atcaccgact gttcgacgta aatatccaat tttgtccgga tgccgaacgg
    10801 cgttgtgccg ccgaccagat agcccgtcca cttgtttgcc tgcgcaggcg cggcgggttc
    10861 gatgtgtttc gcacccaaat gccgcgccag attgcgggtt gaaatctgtt tgtcgccgtg
    10921 catcaagaca atcggcccct taccgttttc atcttgcaaa acaatggttt taatgaccaa
    10981 gtgttcgtct ttgccgaaca gtcgggcaaa ctgcgccgtg ccgccgtgtt cctcataggc
    11041 ataggtaaaa ggttcaaatt cgatgccgtt ttcacgcaaa acgcgtacgg caggtgttat
    11101 cggataagtg tgtttactca tttttcgtca tgcaaaacat ggaaatcggt caagcgcaat
    11161 gttaccatat atccccttta tcgaaggaaa ccgatatgga tatccggtat ttcggcacaa
    11221 cgccccgcta ttccgaagcg gtttgcgcgg gcggcttgat tttcctctcc ggcatggttc
    11281 ccgaaaacgg tgaaacggct gccgaacaga ctgccgacgt gcttgcccaa accgaccgct
    11341 ggctggcgga atgcggctcg gacaaggcac acgttttgga tgcggtcatc tacctgcgtg
    11401 atatgggcga ctatgcggaa atgaacggcg tgtgggacgc ttgggttgcc gccggcagaa
    11461 cgcccgcccg cgcctgcgtg gaagcgcggc tcgcccgtcc ggagtggcgc gtcgaaatca
    11521 aaataaccgc cgtgaagaga gacgcggcaa ctgcataaac agatttcctt atattttcag
    11581 agtacgcaaa tgatgaaaac cttactgccc tttttcggcg tggcgcgcgt cagcggcgaa
    11641 gaccgccaaa ccttcctgca cggacagctt tccaacgaca tcaataatct tcaggccggg
    11701 caggcgtgtt acgcgactta caacacgccc aaaggccgcg tcatcgccaa tatgattgtc
    11761 gtcaaccgtg gagacgattt gctgctgatt atggcgcaag atttgcttga agcaacggtc
    11821 aaacgcctgc ggatgtttgt gttgcgcgcc aaagccgttt ttgaaatcct tgaagattac
    11881 gccgtcggcg cggaattggc agcatccgcc gaaccgcttg ccgcgcaaga acccagtctc
    11941 gcctttacct ccgaatgcgt ctcagacggc atctgttccg tcatcctgca ccatcggggc
    12001 attttgcaca tcgcccccga aaccgcccta cccccttatg acgctgccgc cgaaaacgcg
    12061 tggcggctgc acgaaatccg cagcggttat ccgtggatat gcgcggctac caaagaaacc
    12121 gccgtcgccc aaatgctcaa ccagcacatc atcggcggcg tacacttcaa aaaaggctgc
    12181 tacccgggac aggaaatcat cgcgcgcgcc cagtatcggg ggcaggtcaa acgcggcttg
    12241 gcggtattgt cgggaaattc ggcagccgaa gcgggcatcc tgctgacggc ggacggcgaa
    12301 gaagccggca tcgtactcga cagcgttcaa gattcggaaa actttaccgc ccttgccgtg
    12361 attaaattct ccgccgcgca aaaagaactc accgtcccaa acggcggtat cttcaaagcg
    12421 gttcatctct tctttaaaac caaaaatgca aaataagccc tctaatgccg tctgaagccc
    12481 ttcagacggc attgccgccc ctgttttcgg ttacaatgcc gcatccctaa tttttcaaac
    12541 caccgccatg tccaaaaaaa ccaagcaaga actcgaaaac aacaaactca gcaaacgcct
    12601 gcgccacgcc gtcggcgaca cgattaacga tttcaacatg atcgagccgg gcgacaaaat
    12661 catggtctgc ctctccggcg gcaaagacag ctacgccctg ttagacatcc tgcgccggct
    12721 ccaagccagc gcgccgattg attttgaact ggtcgccgtc aatctcgacc aaaaacagcc
    12781 gggcttcccc gaagaagtat tgccgaccta tctcgaaagc atcggcgttc cctacaaaat
    12841 cgttgaagaa gacacttact ccaccgtcaa acgcgtgttg gacgaaggca aaacgacttg
    12901 ttcgctgtgc agccgcctgc ggcgcggcat cctctaccgc accgcaaaag aattgggctg
    12961 caccaaaatc gccttgggac accaccgcga cgacatcctc gccaccatgt ttttaaatat
    13021 gttttacggc ggcaaactca aagccatgcc gcccaagctg gtgagcgaca acggcgaaca
    13081 catcgtcatc cgcccgctgg cgtatgtgaa agaaaaagac ttaatcaaat acgccgaatt
    13141 gaaacagttc ccgattatcc cgtgcaacct ctgcggctcg cagcccaacc tacaacgcca
    13201 agtcatcggc gatatgctgc gcgattggga caaacgcttc cccggccgta tcgaatcgat
    13261 gttctccgcc ctgcaaaacg tcgtcccttc ccaccttgcc gataccgaac tctttgactt
    13321 tgccggattg gaacgcggtc aaaatctgaa acacggcggc gatttggctt ttgacagcga
    13381 aaaaatgccc gaacgctttt cagacggcag cgaagaagat gagagcgaaa tcaaaatcga
    13441 gccgcaaaaa gccgaacgca aagtcatcaa cattctggcg aacaagccga aaacctgcgg
    13501 cgcgtaaacc tccaccgtca aaaatgccgt ctgaagcccg tttggcgttt cagacggcat
    13561 tttaaaatcc cgaagcagac ggcttccaaa catttgtttc tcagtgttcg ttttcagaat
    13621 gcccgcctgc tggcatattt tgcccgatca gttcggctat cctctcgccg tcaaactgcg
    13681 ttttgaacac caccttgatt tctttggaaa tctcggcaaa cagcttttcg gcatgtttga
    13741 ttttccgctc ttcgcttttt cgcaaatctt ccgccccgtc agtccctttg gcttcaatca
    13801 caaagttcag aatctcgccg cttttggttt tcacgatata ggcaaagtcg ggcgaatacg
    13861 tgccgccgcc ggcaacaggg attttgatgg agtttctcgg tattttggta aatacgatta
    13921 cgccttcaat ttggttgttg gcgacatttt catgttctat atccgaatcg tagaaaatct
    13981 cgccgaagag atagccggcg gcaggccggt gctccgtatc ttcaaatctg cccaaatctg
    14041 cttttttcac cgcgcgcggt ttgccgtctt tattggtaaa tttggtcgga tggattttgc
    14101 tgccgacaag ccggtaatcc agttcgaatt tatggaagga atgatgaagc aaaaaccggt
    14161 tgaagccgtt tttgatttgg gcgatggtct gcatattcaa aaaatcgcca atgttcagtt
    14221 cgtcgcggac gcggtaaaac gcctgatgca aagtctgcat ctggattttt gccgtttgtg
    14281 ccagtttttc cagaaactca cggtaagtca ttgtgttgaa acggataaaa tcttcatctt
    14341 caatactgtc tatgcggcgg gaaagcataa gcccgttgtt gatataagct tcgtttaccg
    14401 ccgtgcatat gcctgcctgc gggaatttgg cggcgttttc acgcaaatag gcggtaaata
    14461 aatcgacaaa ttcggcttca tctttgattt tgtactgcaa aacggcttta tggtgaatca
    14521 gctcccacaa ggctttgagt tcttcatatt tgccttcgcg catgatgatg gtgtctttgc
    14581 cctcgtcttt ggcgttgctg actttgcctt tgtccaaacc tttggggaag gcttcgggat
    14641 aggcggcttt taatttgtca tagccgtctt cggcaaagtt ttcattgtcg tcaatgatgc
    14701 cgtctgaaaa cagttggttt accaatacca gcggtttgat atcggggtat ttttgcaata
    14761 ttttttgttt cagctcttcg gtaaactttt tggagatttc ttcctgaaaa gaattgtcgt
    14821 tgatttcgcc gacaagctgc ttcacaaagt ctttttcgct gctatcgaca aaataattca
    14881 gtttgtacgg tacatcgcgc acccgcgcca tcagctcgtt taccggcagg cgcaggccgc
    14941 gtccgacttc ttgcagcttg gaagtcgtgc tgccgctgga acgcagtttg caaatctgga
    15001 aaacgttggg attgtcccag ccttcgcgca gcgtccattt ggaaaaaata aagcggcgcg
    15061 ggttgtccaa agacagcagt ttttccttat cgtgcaggat ttcattgatt tcctgctcga
    15121 ttctatcgtc gctgtctgta ttgtctttgg aaaaatagcc gccgtggcag gcggatacat
    15181 cgtccaacgt cttttgcagg taatcgcggt aaaacgggtc gctttccgtt ttcagacggc
    15241 gtgccgcttc cgcgcgaatc cagctttcaa atttatcttt caggctgcct gaaagctcgt
    15301 tgccgctgcg gtagcccgcg atatcgtcaa taaaaaacag cgtcagcggc ttgattttgg
    15361 gctgtggcgc gcgttctgcc aaaagcgcgc gttccagctt gaaatgttcg gcaaccgccc
    15421 gctgcatcat cgcatcctgc accgtttgcg aataggaata agggttgatg acggcacccg
    15481 ttttcaactc caagccgttg cttaacacca ccacggtttt attcattttg tcgattttca
    15541 aatccgaaat agccggatgg atttgcgcca aatcttcgcc ttttgccagt ttgaacgtct
    15601 gctttttgtc cttttcgttt aattcaaatt tcgcttcttt gccgtccgac gacaccagtt
    15661 ttaccgccgc atccatgccg ccctgcattt cttcctgaaa cacgcgcacg cctttgacca
    15721 gcccgtcgtt aaacgcgtct actgccgtca aacggtaaag caagttgtaa tattcatcgt
    15781 taaatgttgc accgtagcgc aaaatatatt gcggttttaa gcgtttgata ttgccccacg
    15841 ttttcgcgct atctcgggtc gggaatttat gcggttcgtc cacaatcata aacgggcgca
    15901 cggcagccaa tgcatcaacg ggattgtcaa acaaatcctt caatgccttg tcgcccgtat
    15961 cgttcatgga cgacgaatta accatgcccg cgttaatcag cagcacatga atttcctttt
    16021 tgttttccgc tttgacaaat tgctcaatcg ttatgggcgc attggacttt ttgcccttat
    16081 tctttttcgc gctttccacc acataggttt tcaggcgtac gccttcataa tcgccgccga
    16141 aatcctgttc aaaatgctct gccaaagcct tgctttgcaa aaactgctgt gttcccgcct
    16201 taatggacaa agtcggcacg accacgataa atttgaacac gcccagccaa cggtgcagct
    16261 cgaacatggt ttgtgtgtag gtataggttt tgcccgtgcc cgtttccatg gaaatatcaa
    16321 ggatattttg gtcgtccgaa cggtcgggga atcggccgtc tataccgttt tggctttgca
    16381 ctttcaggat attgtttgcg tattgttttg cagcaaacaa aagttcggga ttttcgtctg
    16441 ccgtccgata tttgggcgtt gccccgtcaa acacgcccaa aaccgccgaa accgcccgca
    16501 tttggtgcgg ctggtttttc tcgtaattaa aaccgctcat gaatgtttct ccatgaagtc
    16561 attgttgtat cgtccttccc ccgcaagggg aatccaagcc gaaaagcagc ctgcactttc
    16621 ggattccggt tacaccctga taatcacatt caactcaatc tcttttttat tggtataacc
    16681 gcgaaccgcc tggtcgagtt cgtgctgcat ggcgcttgcc atattgctgc cgaatacaat
    16741 cacacggttg ggattgaaat ccgcatcgtc gtccagcttc ttgataaacg ccagcaaatc
    16801 ggcggaagta aaacccttgt taagcagata cagccgtttt tcgcacagat acgccgtata
    16861 actgcccaac cgcacaggct caaccggcgt ggtcagtgcc gccccgtcat acagcgtcca
    16921 ggtggtcaga agcgtttgca gctgttcttc gcttaattca tcgttaagcg gcaaatccgg
    16981 ttgttcgggc gaaaaatcct tgtctggatg ctgcctgaaa ttgtctgccg tttgaaagat
    17041 tttgaagccc gaatcgcccg tgtaatcggg atgttcgacg cggattttgg cggcgacttt
    17101 ttctatgcgg gctttggtga tgtcgaagat ggtcgggtag cctgctttac gggcttcgga
    17161 tttttcagcg gttttttcgg gaagctgtac acagatatag cggcggttac cgttttgtcc
    17221 ttcggcgtta agctgcatca cggcgtgggc ggttgtgccg ctgcctgcga agaagtctag
    17281 gattaggtca ttactctttg aacttattga aactaaaaat ttaatcaatt gactaggctt
    17341 ggggaaggta aatattttgc taccaaataa atctgtgatt tcttttgtgc cttctttagt
    17401 cattccgata ttttcaggta gcgtcctact aaaaatagcc aaatattcgg ctactgcatc
    17461 ggctaatgta ccaacatttt caggtaatct actgcttaca gctactttgc caaaatcctc
    17521 gccagctttt ttcatgtcgt catctttaaa gtaacgcata actggattgc tgatatttaa
    17581 gaaatcataa tcatcaggga aaacgatttt tcctttatta taataatctt gaaatgtatc
    17641 tttggttaca cgccaagttg catttggatt tgctggatat ttttttcctg tcttgggatc
    17701 aaccattgtg aaaaaactat ttggcctttc cgccgcagtt gtttgtttcg ttaagtcgtg
    17761 ggtacgccaa ggacgatcgg ggaaatcatc agtctcataa tagcgtcgtt ccttgccttt
    17821 agttgctgca ataaattggc aagattttgc gaatacaaat atccattcat aatcctgcga
    17881 aataccaaaa ggcacatctg atttagctgt tctttttcgc caaggcaatt gtgcaacaaa
    17941 attcccttcc ccaaacactt catcacacaa caatttcaac tgcgccgctt cgttatcgtc
    18001 aatcgagata aaaatcacgc cgtcgtcctt taacagttcg cgggcgatat acaggcgcgg
    18061 atacataaag gtcagccacg cgctgtgcga gtttgagcct ttgtcggtga aatctaaaat
    18121 ccgcgcggct tcgtcttcgt caatattcgc caggagggca agttcggcgg gtgtgaattt
    18181 gcggtcgtcc tgatagacaa agccgtccga tccggtgttg tagggcgggt cgatgtaaat
    18241 catcttcacg ctgtttgcgt aggcgttttt taagtgtttc aacacttcca gattatcgcc
    18301 gcgaatcagc agattttggc tgcctgcatt ttcgggcttg gcgttgtgcg ttttgtcttc
    18361 acttatcagg gtttcgggcg gcaggttgcg aagcaggcgg gcatatgatt tgcccagcca
    18421 gttcatttcg taaaattcgc gtccgatgtc ggtctgtggc gcaatttcgg cttgtaatct
    18481 gtcgataagg aaatttccgt ctgcgtcaaa acaggcggga aacagttttt tgagctgttc
    18541 gagttgggta gagttggcgg taatgccgtc tgaagtgtag attgcctcag tgttcgcccc
    18601 ggctgtgtcg cttagatcag ggc
//
LOCUS       DAXDXW010000042        18407 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXDXW010000042 DAXDXW010000000
VERSION     DAXDXW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18407
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.043"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            42..115
                     /locus_tag="ACFB64_001230"
     tRNA            42..115
                     /locus_tag="ACFB64_001230"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Cys,seq:gca)
     gene            216..305
                     /locus_tag="ACFB64_001231"
     tRNA            216..305
                     /locus_tag="ACFB64_001231"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:250..252,aa:Leu,seq:taa)
     gene            409..1461
                     /gene="sohB"
                     /locus_tag="ACFB64_001232"
     CDS             409..1461
                     /gene="sohB"
                     /locus_tag="ACFB64_001232"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC1209591.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFETFFLSEEETKHQEKKEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIIGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPGLDIEKI
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKRSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(1512..2366)
                     /locus_tag="ACFB64_001233"
     CDS             complement(1512..2366)
                     /locus_tag="ACFB64_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HFC1209592.1"
                     /translation="MWKMLKHIAKTHRKRLIGTFSPVGLENLLMLGYPVFGGWAINAV
                     IAGRVWQALLYALVVFLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLERDNHFDERQLYRHYGLVSRLRVLISNREAFGYLCVGAAMG
                     ILFGFAFVMMTLKGYGSAGHIYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRIEWS
                     ERNIKAGT"
     gene            complement(2377..2808)
                     /locus_tag="ACFB64_001234"
     CDS             complement(2377..2808)
                     /locus_tag="ACFB64_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFC1209593.1"
                     /translation="MNRLDQLGIRINLICNVFDKWIGQQDLNYNLFTVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRERLLSLTEKGKVHAAPLTE
                     NAQEFSDKVFSTFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            complement(3043..3912)
                     /locus_tag="ACFB64_001235"
     CDS             complement(3043..3912)
                     /locus_tag="ACFB64_001235"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC1209594.1"
                     /translation="MSAETYTQIGWVGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGSTAELVRACPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(3988..4899)
                     /gene="hisB"
                     /locus_tag="ACFB64_001236"
     CDS             complement(3988..4899)
                     /gene="hisB"
                     /locus_tag="ACFB64_001236"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC1209595.1"
                     /translation="MTKTQLHLNNFLTLAQEAGSLPKLAKLCGYRTPVALYKLKQRLE
                     KQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETRIAETA
                     SAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDIDIS
                     CKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLSGR
                     PGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFKAF
                     GRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(4916..5995)
                     /gene="hisC"
                     /locus_tag="ACFB64_001237"
     CDS             complement(4916..5995)
                     /gene="hisC"
                     /locus_tag="ACFB64_001237"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC1209596.1"
                     /translation="MKSVRSFIRNDILAMSAYKITDVPPGFAKLDAMESPAHPFEGHE
                     ALMREWRAQLASAPIHLYPNPSGCGLQEALRSAFDIPDCAAVALGNGSDELIQFITML
                     TAKPGAAMLAAEPGFIMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAAIEASDGIVVVDEAYGAFNGDSFLPQAGRIPNLIVLRTL
                     SKIGFAGLRIGYATGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNAFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITIGSSAQNDAVLDVIRRLYR"
     gene            complement(6041..7330)
                     /gene="hisD"
                     /locus_tag="ACFB64_001238"
     CDS             complement(6041..7330)
                     /gene="hisD"
                     /locus_tag="ACFB64_001238"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC1209597.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAIAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILVKDLNEACEISNYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(7327..8214)
                     /locus_tag="ACFB64_001239"
     CDS             complement(7327..8214)
                     /locus_tag="ACFB64_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC1209598.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLSEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMTEETKNGRKQTRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGGKAYIRPKTE
                     QETLQTIPSPKITKENPKS"
     gene            complement(8306..8971)
                     /gene="hisG"
                     /locus_tag="ACFB64_001240"
     CDS             complement(8306..8971)
                     /gene="hisG"
                     /locus_tag="ACFB64_001240"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC1209599.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIAPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSYLVVNKAALKTKYALLEPIIQSFGGAVKAKW
                     AFI"
     gene            complement(9050..9703)
                     /locus_tag="ACFB64_001241"
     CDS             complement(9050..9703)
                     /locus_tag="ACFB64_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC1209600.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASAPAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKINQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            9979..10063
                     /locus_tag="ACFB64_001242"
     tRNA            9979..10063
                     /locus_tag="ACFB64_001242"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10013..10015,aa:Leu,seq:caa)
     gene            complement(10126..10233)
                     /locus_tag="ACFB64_001243"
                     /pseudo
     CDS             complement(10126..10233)
                     /locus_tag="ACFB64_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            10757..12484
                     /gene="ilvB"
                     /locus_tag="ACFB64_001244"
     CDS             10757..12484
                     /gene="ilvB"
                     /locus_tag="ACFB64_001244"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC1209601.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELVETIKKAFQIAASGRPGPVVLDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNILSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRTDGKA"
     gene            12495..12986
                     /gene="ilvN"
                     /locus_tag="ACFB64_001245"
     CDS             12495..12986
                     /gene="ilvN"
                     /locus_tag="ACFB64_001245"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC1209602.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            13053..13346
                     /locus_tag="ACFB64_001246"
     CDS             13053..13346
                     /locus_tag="ACFB64_001246"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC1209603.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYNLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAV"
     gene            13426..14439
                     /gene="ilvC"
                     /locus_tag="ACFB64_001247"
     CDS             13426..14439
                     /gene="ilvC"
                     /locus_tag="ACFB64_001247"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC1209604.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRHGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVAELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGARLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(14558..15553)
                     /locus_tag="ACFB64_001248"
     CDS             complement(14558..15553)
                     /locus_tag="ACFB64_001248"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC1209605.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKDAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGADATYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFAQETVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKGVGFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPAGIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(15695..18280)
                     /gene="acnB"
                     /locus_tag="ACFB64_001249"
     CDS             complement(15695..18280)
                     /gene="acnB"
                     /locus_tag="ACFB64_001249"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC1209606.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKNPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLVSPKYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVNILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFHLPQAPAESKVGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVIPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRALERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKSDIPVRLWVAPP
                     TKMDAKELSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
BASE COUNT         4119 a         4483 c         5132 g         4673 t
ORIGIN      
        1 ctcgtttccc gctccaagtt ttgtagacaa gccagtttta gggcgagata gcaaagtggt
       61 tatgcagcgg attgcaaatc cgtctacgcc ggttcgattc cgactctcgc ctccatatat
      121 cttacggcgg ggtggcagag tgtttatgct atgaggggtg caaccttcat ataggccggt
      181 taaaatccgc gcccccgcct ccacctttca caaatgcccg ggtggtgaaa taggtagaca
      241 caacggactt aaaatccgtc gggactaaac atcccatgcc ggttcgattc cggctccggg
      301 caccaagctg aaaatgacaa tgccgctcca agcggttatt tttttatctg tcggatgggg
      361 atggacaata ttgatatgtt catgcctgaa caagaggaaa tccaatcaat gtggaaagaa
      421 attttactga attacggtat tttcctgctc gaactgctta ccgtgttcgg cgcaattgcg
      481 ctgattgtgt tggctatcgt acagagtaag aaacagtcgg aaagcggcag tgtcgtactg
      541 acagattttt cggaaaatta taaaaaacag cggcaatcgt ttgaaacatt ctttttaagc
      601 gaggaagaga caaaacatca ggaaaaaaaa gaaaagaaaa aggaaaaggc ggaagccaaa
      661 gcagagaaaa agcgtttgaa ggagggcggg gagaaatctg ccgaaacgca aaaatcccgc
      721 ctttttgtgt tggattttga cggcgatttg tatgcacacg ccgtagaatc cttgcgtcat
      781 gagattacgg cggtgctttt gattgccaag cctgaagatg aggttctgct cagattggaa
      841 agtccgggcg gcgtggttca cggttacggt ttggcggctt cgcagcttag gcgtttgcgc
      901 gaacgcaata ttccgctgac cgtcgccgtc gataaggtcg cggcaagcgg cggctatatg
      961 atggcgtgtg tggcggataa aattgtttcc gctccgtttg cgattatcgg ttcggtgggt
     1021 gtggtggcgg aagtgccgaa tatccaccgc ctgttgaaaa aacatgatat tgatgtggat
     1081 gtgatgacgg cgggcgaatt taagcgcacg gttactttta tgggtgaaaa tacggaaaag
     1141 ggcaaacaga aattccggca ggaactggag gaaacgcatc agttgttcaa gcagtttgtc
     1201 agtgaaaacc gccccgggtt ggatattgaa aaaatagcga cgggcgagca ttggttcggc
     1261 cggcaggcgt tggcgttgaa cttgattgac gagatttcga ccagtgatga tttgttgttg
     1321 aaagcgtttg aaaacaaaca ggttatcgaa gtgaaatatc aggagaagcg aagcctgatc
     1381 cagcgcattg gtttgcaggc ggaagcttcc gttgaaaagt tgtttgccaa acttgtcaac
     1441 cggcgagcgg atgtgatgta gtttgccgaa cggatggaaa tgccgtctga agtgtgttca
     1501 gacggcattt ttcaagttcc ggctttgatg ttccgttccg accactctat ccgttgtccg
     1561 atgtctttca aattggaata ttgttcgacc aatcgcggca catcgtccaa actcatggca
     1621 aacatccaca gataagtgcc gaccgaataa atatgccccg cgctgccgta gcctttgagc
     1681 gtcatcatca caaaagcaaa gccgaacaaa atacccatcg ccgcgccgac gcagagatag
     1741 ccgaaggctt cgcggttgga aatcagcaca cgcaggcgcg aaaccagtcc gtaatggcgg
     1801 tacagctgcc gctcgtcgaa gtggttgtcg cgttccaagc tgttgttcag gcggaaatac
     1861 aggttttcgc tgatggcggc aaaacgtggc aaaagccata aaaacaacgc aagtatgccc
     1921 accgccgaca cgccgaccca aaattccagc accagcagca tgatgcacgc gccgaatatg
     1981 gatacgacgg atgtcgcggc aatcggcagg tgttcttcaa aaaagctgac aaattcacgc
     2041 gacagggcaa cccgtgcagt taccgctgaa tgcgggactt gccgctgccg ttgttccaac
     2101 acaaccggca cggcgatttc ggtataaatc cgcgtaaacg tgcgcgtatc ggcaatccgc
     2161 cgtgccgcac cgaccagcca catcaaaaat acaaccaaag cgtacagcaa cgcctgccac
     2221 accctccccg caatcacggc attaatcgcc cagccgccaa acaccggata ccccagcatc
     2281 aaaaggtttt ccagtccgac cggggaaaat gtgccaatca atcgcttgcg gtgggttttg
     2341 gctatgtgtt tcaacatttt ccacatattt gtccctctat tttttatttt ccgagattgt
     2401 tttttccatc acttccgcca gtgcatccaa atcggcaaac agccgggttg tgcgcttgtc
     2461 gccgaatgtg gaaaatactt tgtcgctgaa ttcctgcgcg ttttctgtta aaggtgcggc
     2521 atggactttg cctttttcgg tcagcgacag caaccgttcg cgcctgtcct gttcgccttc
     2581 ctgccattca atcaaccctt gtccggcaag ggttttgcat acgcctgaaa cggtctgttt
     2641 gggcagactc cactcttctc cgatgtgctt ttgcgtgcgg ctgccttcgg ttgccagggt
     2701 ataaagtacg gtaaagaggt tgtaattcaa atcctgctgc ccgatccatt tgtcaaagac
     2761 attgcaaatc aggttgatac ggattccaag ttggtcgagt cggttcataa ttggtcttga
     2821 tattgactaa atttgtgcgg attatagtgg attaacaaaa accggtacgg cgttgcctcg
     2881 ccttgtcgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
     2941 cactatatag tcataatcgg gactgatttc aagcgtgggg cgataaaaat gccgtctgaa
     3001 acgctttctg gcttcagacg gcatattgtt tggaaaggga agtcagtgtt ctgccaattt
     3061 caggtaaacg ccggaaacgt cctgttcgcc gtagccggct tcaactgctt tgcggtagct
     3121 ggcagcaacg gtttcgacgg cgggcagggt gttgcctgcc tgttcaagct ctttgacggc
     3181 gaggttaagg tctttggaag cgtgtttgag tgcaaaggca ggggggaact cacggtttgc
     3241 ccatagtgat ttttttgttt gaaacatagg cgagtccatt gccgagccgc cgatggcttc
     3301 gacgatggtg tcggtatcga tgccgaactg ccgcgccatc agcatcgctt cgctgtacgc
     3361 ttcgccgaaa atgcctaaga gcgagttcaa gacgagtttc gcgcccgagc ctttgccgac
     3421 atcgccgaaa tggaaggttt ttttgccgac aagggaaaat attttttgca gcgggtttaa
     3481 aacggcttcg ctgccgccga acagaatcag cagtgtgccg ttggtggcgg gtccgaccga
     3541 tccggaaacg ggtgcttcgg caaactgtcc gcctgcggct tcgacaagtg ctttgacggc
     3601 gaggttttcg gtcggggaga tggtgctcat gttgacgatg attttgccgg ccaatccgtc
     3661 gcggactccg ttcaggatgt cgcacacggc ggcatagtcg gaaaccatca ggaaaatgac
     3721 gggacaggcg cggacgagtt cggcggtgct gccgtaaact tttgctcctt tggcggagat
     3781 gggggcagtt ttgtcgggcg agcggttgta tacgccgact tcgatgccgc cgtccaagag
     3841 ccgcgttacc ataggcagac ccatttgccc taagcctacc cagccgattt gtgtgtatgt
     3901 ttctgcggac atagtgtttc tcctttgttg gggcgctgta atgccgtctg aaggcttcct
     3961 gcgcttcaga cggtctgttt ggggatgtta tgcggtcagc gtgcctttgg tcgagggggt
     4021 ttgcccagcc attctcgggt cgtgttcgac tgccatacgc agggcgcgcc cgaaggcttt
     4081 gaatacggtt tccgcctgat ggtgggcgtt tttgccgctg aggttgtcga tgtgcagggt
     4141 catcatgctg tggttgacga tgccgtggaa aaattcttca aacaaatcga catcgaaacg
     4201 tccgattagt gcgcgggtaa attcgatgtt gtacacgagt ccggggcggc cggaaaggtc
     4261 gatgacgacg cggctgaggg cttcgtcgag cgggacgtag gaatgtccgt aacggcggat
     4321 gccttttttg tcgccgagtg cctgccggat tgcttgtccg agtgtgatgc cgatgtcttc
     4381 ggcggtgtgg tggtcgtcga tgtgcaggtc gcctttgcag ctgatgtcga tgtcaatcat
     4441 gccgtggcgg gcgatttggt cgatcatgtg ttcgaggaag ggaacgccgg tatccagcct
     4501 gcttttgccg ctgccgtcga ggttgatgga gacggtgatt tgggtttcgc aggtattgcg
     4561 gttgacggta acgctgcggc agccggcagc agatgcggtt tcggcaattc gggtttcggc
     4621 agttccggtg ctttctgcgg cggtttcggg gacagtgcgt tcgcggtgtt ttctgtcgag
     4681 ccagcctttg ggtttgccgg tgtgtttttc gagttttgcc atcaggctgg gacggatgcc
     4741 gcgcgcatcg gggtcttctg cctgcttttc aaggcgttgt ttgagtttgt aaagtgcgac
     4801 gggggtacgg tagccgcaga gtttggcaag cttgggcagc gaacctgctt cttgggcgag
     4861 agtcaggaag ttgttcaggt ggagttgtgt cttggtcata gggattccgt ttggtttatc
     4921 ggtagagccg gcggataacg tcgaggacgg catcgttttg tgcggagctg cctatggtaa
     4981 tgcgcaggca gtgttccaaa agcggatgtg cgccgtgcag ttttttgacg aggatgcggt
     5041 tttgtttgag tgtgtcaaac aacaaatcgg catcgggtac gcgtatggta atgaagtttg
     5101 cctgacttgg aaaggcgttc agacggcata ttttgcccaa ttcggcgaac atccgttcgc
     5161 gttcgttttt caggctgtcg atgttggcag aaatgatgcc gtagtgttgc agggcgagtt
     5221 tggcggtggt caggctcagt tggttcatat tgtagggcgg caggattttt tgcagttcgc
     5281 cgatgacttc gggacagcct gtcgcataac cgatacgcag tccggcaaaa ccgattttgc
     5341 tgagggtgcg taagactatc aggttgggaa tcctgcccgc ctgcggcagg aagctgtcgc
     5401 cgttgaatgc gccgtaggct tcgtcgacga cgacgatgcc gtctgaagct tcgatggcgg
     5461 cttcgatttc ggcacgcgtg aaacatacgc cggtcgggtt gttcgggtag gcgataaagg
     5521 tcagggcagg gcggtgcttc ctgacggctt cgaggacggc gggcaggttg agggtgaaat
     5581 ctccgttcag tggaacgccg acataatcca tgccgtacag cgcggcgttg tggcggtaca
     5641 tgatgaaacc gggttcggct gccaacattg ccgcgccggg tttggcggtc agcatggtga
     5701 tgaactgtat cagttcgtcc gaaccgttgc ctaaggcgac ggcggcgcag tcagggatgt
     5761 cgaacgccga acgcaatgct tcctgcaaac cgcagccgga ggggttggga tagaggtgga
     5821 tgggggcgga tgccagttgc gcccgccatt cccgcatcag ggcttcgtgc ccctcaaaag
     5881 gatgggcggg gctttccatc gcatcgagtt tggcaaaacc gggcggaaca tcggtgattt
     5941 tgtatgcgga catggcgagg atgtcgttgc ggatgaagga gcggacggat ttcatcgtgt
     6001 ttccttaatg gttggaatat gcctgtacgc cgtttcggca ttatttcata cggaactctg
     6061 ccgcgcgtgc gtgggcggtc aggctttcgc cgtgtgccag cacgctggcg gtttcgccta
     6121 atttttgcgc gccctgttcc gaaacctgaa tcaggctgga gcgtttttgg aaatcatatg
     6181 tccccaaagg cgaggaaaag cgggcggttc ggctggtggg caacacatgg ttcgggccgg
     6241 cgcagtagtc gccgaggctt tcgccggtgt agcgtcccat gaaaatcgcg ccggcatggc
     6301 ggattttttt cgcccattcc tgcgggtttt cgactgacag ttccaagtgt tcgggggaaa
     6361 tgtagttgga gatttcgcag gcttcgttca agtctttaac gagtatcatc gcgcccctgt
     6421 tgccgagcga ggcctcgatg atgtcgcggc gcggcatggt ttcgatgagc ctgtccatgg
     6481 cggcttctac ttcgtcgaga tacgcttgcg acgtgccgat gaggatggct tgggcaattt
     6541 cgtcgtgttc ggcctggctg aacaaatcca tcgccaccca atcggcgggt gtcgtgccgt
     6601 cggcgatgac cagtatttca gacggccccg ccaccatgtc gatgccgacc acgccgaaca
     6661 cgcggcgttt ggcggcggcg acgaaggcgt tgcccggacc ggtgatttta tcgacttggg
     6721 ggatggtttc cgtgccgtag gcgagggcgg cgatggcttg cgcgccgccg acggtgaaga
     6781 ctttggttac gccggcgacg tatgcggcgg caagcacgat gtcgttgcgt tcgcctttcg
     6841 gtgtcggtac gaccataatg atttctttca cgcctgcgac gtgggcgggc atcgcgttca
     6901 tgatgacgga actcggatat gccgccttgc cgcctgggac gtaaatgccg acgcggtcaa
     6961 gcggggtaat ctgttgcccc agcagcgtgc cgtcttcatc ggtgtagctc cacgattcca
     7021 ttttttggcg ttggtggtag ctttcgacac ggcgggcggc ggtctgcaat gctgtctgaa
     7081 cgtcgttcgg aatgcgctcg aacgccgcgt tcaaatcggc ttgcgtgagt attaaatcat
     7141 cgatgctttt agcgtttgtc tgatcgaatt tgttggtgta ttcaatcaaa gccgcatcgc
     7201 cgcgcttttg cacgtcggcg caaatgtcgg cgacgatgcg ttcggtttcg gggttttgcg
     7261 cggtttcaaa agccagcagg gctttgagtc cggcttggaa atcgggcgat tgggtgttga
     7321 gttttttcat gatttgggat tctcctttgt tattttggga cttgggatcg tctgaagcgt
     7381 ctcttgctcg gttttgggtc tgatatacgc ttttcctccc ccgctaccaa acttcaattc
     7441 gggcaggcat ttttgttgca gaccgagttt ggctaatacc tcttcgctac gagaggtagt
     7501 acggaaacgg taacgggttt gttttctgcc gtttttggtt tcctcagtca ttgtttcaaa
     7561 accgtccact tcgtacgcac tgacaacagc gttttgcaga ccggtgtgaa cgccgataac
     7621 gtatttcact tttgaagcaa catctttacc gataacccag ttgcccaacg tacgcgattt
     7681 gaggttggca tcgtcttggt tgtcgaaaag gtagtctaat tcttcgtcag tatctaaatc
     7741 gaaagcattg tggatcttga tggcgagaat cagcccgtcg gggttaatct cgctaagtgg
     7801 gagagaagag aatccaaagc ggcgatctag ttcttctacg ctaataccac ccggaccatg
     7861 cccggcaatt ttatttttga gttttttacc caagacagat ttaacaaaat gaattaaggc
     7921 agattcggcg gcgagcgctt cgacttcagt cagatgatag ctgatgatat agcgaccgag
     7981 tttcttgcat ttggagatgg ctttgagttt ccgatcgata atctcgccgg agactgggtc
     8041 ttgtgaacga ctagcaaccc attcatgctc gaagatacga ttaccgcaac cttttccgat
     8101 gtagaggatt ttgtcttttt tcaggtcggt cagacaatag acataaaacc gacgctcgcc
     8161 gttattcaaa acggaaaggg tcgaatctga gaattttttt atttttgcaa ccattttaac
     8221 ttctcctata cagtttactg ccggtgatat tttcagacgg cctgtcggcg ggaacggata
     8281 gggtcgtctg aaaacgcatc tttattcaaa tgaatgccca cttcgccttc actgcgccgc
     8341 cgaacgactg aataatcggc tccagcagcg cgtatttcgt tttcaaagca gccttgttga
     8401 ccaccaggta gctggaaatg tcgacgatgt gttcgactgc ctccaagccg tttgccttca
     8461 aggtgtttcc cgtcgaaacc aagtccacaa tcgcatcgct caagccgacc agcggcgcaa
     8521 gttccatcga gccgtacagt ttgataatgt ccacatggac acccttgccg gcaaaatgag
     8581 atgccgcgat ttcaggatac ttcgtggcaa tcttcagacg gcatccgggt tgcgaagcgg
     8641 cttcgtaatc aaacccttta cgcacagcaa ccatcatgcg gcacttggca atctccaaat
     8701 ccaaaggccg gtaaagcccc gtgccgccgt gttcgatcag cacgtctttg cccgcaatgc
     8761 cgaagtccgc cgcgccgtag cggacataag tcggcacatc ggttgcgcgg acaatgacaa
     8821 ggcggatgtt ttcatggttc gtcccgataa tcagcttgcg cgatttttcg ggctcttcag
     8881 tcggagcaat gccggcagcg gcaagcagcg gcagcgtctc ctcaaaaatg cgccctttgg
     8941 ataaggcgat ggtcaaagca ttatcctgca tggtgtggat gtcctttgtc ggttgaaaca
     9001 ataatgccgt ctgaacaggc gccggcggtg cagacggcat acggttttgt cagagcaggg
     9061 tccttacatc ggcagcaatc gtttccggct cgcttccgta aggcgagaaa atggcaacct
     9121 caccgttttt gtcgatgaga tacgcaccgg aagagtggtc gaccaaatag ttttcgctgt
     9181 cgtctttttg attgattttg gcagaaacca cgcggtattg ctgcttgatg accggcaggt
     9241 tttgaccgcc cgttgccgtc agaccgataa agtccggatt gaactgtttg gcatacttgc
     9301 cgatgatttc aggcgtgtcg cgttccggat cgatgctgac gaacaccact ttcacgtcct
     9361 tagcctgccc gcccaactgc ttcaaagtgt cgctgtacgt caaaagctct gtcgggcaga
     9421 catcgggaca gtgcgtaaag ccgaaagaca gaatcacgac cttgcctttc aaatcgctca
     9481 ggctgaaagg cttgccttcg ccgtcggtca gcgtgaaatc gccgccgatg tcttccttac
     9541 gcatatccgt accgcgcgtt tgcggctttg ccgcattttc cgcagccggc gcggatgcac
     9601 ttgaagaagc ggcttgcgcc gcactgttgt cttgaggttt gcaggcggca agcgcggcaa
     9661 gtacgaaaac gcccggcaaa aaggaacgcg gtacggaaaa catgacaatc tcccaagaat
     9721 aaatagaaaa acctgattcg tacggaattt gaatatttcc atcttaacgc gtttggcaga
     9781 tttaatgtag tattttttta cgttgtccgt acggcaggaa ttgccgaatc atacagaatc
     9841 tttggagcgg aaagaacagt aagattaaaa taataaaaat tctatttaaa atcaaaaaca
     9901 aaataaaaac agataaaata atttcaaaaa aaggtttaca gaatctcaaa atccattata
     9961 atggcaattt cttcaccagc ccaggtggcg aaattggtag acgcagggga ctcaaaatcc
    10021 cccgccgcaa ggtgtgtcgg ttcgagtccg accctgggca ccacaaccgc ttttggagcg
    10081 gttatttttt tgtctattgc tgtttaatga aatagataag actaaatata gtggattaaa
    10141 tttaaaccgg tacggcgttg cctcgccttg ccgtaccatc tgtactgtct gcggcttcgt
    10201 cgttttgtcc tgatttaaat ttaatccact atacaatcaa tatatttaag tcgttgttag
    10261 ccctcttggt tctgtcaatg ccgtccggtg caatgagcca ttttcaggaa gtatggttgg
    10321 gaatattttt gatatatcaa aaataaaatc aagttcaatc aatatcccga gtgaaaccct
    10381 gtaaatttta aaattcttga tactttcaca tctgccccca tgcaaactgt caacaatttc
    10441 catctttcaa aaaaggtact cctgatttta ttcttttaaa atcaatcaat aatttctccg
    10501 ctttcaaaaa aatttctccg ctttcaaaaa atattaaaag atgaaagatt tttcttgatt
    10561 gaactgtcgg tttgcgttat cgtttttact ctttttcaca aactctgtgt tcctttccaa
    10621 ttgattggat aatctgtctg ttgccgtgtt cccccaatgc gcgtacccta aatcgctgct
    10681 tggtggaatt gcattacagg tgctgtggca aagcggtttg tccctattgg tttgaaaccg
    10741 tataaaagag gtcgttatgc agttatcagg cgcgcaaatc atagtgcaga gtctcaaagc
    10801 cgaaggcgtc gagtacgttt tcggttatcc cggcggtgcg gttatcgaaa tctacgatgc
    10861 cctgttccaa ctcaataaat tcaaacacat tcttacccgc catgagcagg cggcagtaca
    10921 cgcggcagat gcgtatgcgc gcgtcagcgg taaggtgggc gtggcattgg ttacatccgg
    10981 cccgggcgtt accaatgcgc tgaccggtat tgctactgcc tatacggatt cgattccgat
    11041 ggtggtcatc agcggacagg taggcaattc cctgattggt acggatgcgt tccaagaagt
    11101 tgatacggtg ggtattaccc gtccgtgcgt caaacacaat ttcttggtta cggacatcaa
    11161 tgagttggtg gaaaccatta aaaaggcgtt ccaaattgcc gcaagcggcc gaccggggcc
    11221 cgtggtgctt gatgtcccga aagatgttac gcaggcgatg gcgaaattca gctatcctca
    11281 ggaagacatt tttatccgtt cgtatcaacc ggttgttcaa gggcatatcg gtcagattaa
    11341 aaaggccgtg cagatgttgg catctgccaa acgcccggtc gtctattttg gcggcggcgt
    11401 ggtgttgggt aatgcttctg aagagctgac ccgatttgtc cgaatgacgg gtgctccgtg
    11461 cacgggtacg ctgatgggct tgggcgctta tccttccggc gaccgccaat tcctcggtat
    11521 gctcggtatg cacggtactt acgaggcaaa ccttgccatg cagaatgcgg atgtcgttct
    11581 tgccgtaggt gcgcgttttg acgaccgtgt ggtttccgta ccgtccaaat ttttcgagaa
    11641 ggcgaagaag gttatccata tcgatgtcga tccttccagc atcgccaaac gcgtgaaggc
    11701 ggacattccg attgtcggcg acgtgaaaaa cattttgtcc gagatggttg cgctgtggca
    11761 aaaacaagag tccgtgccgt ctgaagatgc tttgggcaaa tggtggaaaa ccatagagga
    11821 atggcgttcc cgcgattgct tgtggtttga caacggcagc gaaattatca agccccaata
    11881 tgtgattcag aagcttgccg agattaccgg caattcggca atcatcacat cggatgtagg
    11941 gcagcatcaa atgtttgcgg ctcaatatta tcctttcgaa cgtccgcgcc aatggctcaa
    12001 ctccggcggt ttgggtacga tgggcgtggg tctgccttat gcgattggtg caaaacttgc
    12061 cgccccggat caagacgtat tctgtattac cggcgacggc tcgatccaga tgaacatcca
    12121 agagttgtcc acctgtttcc aatatcggat tccggttaac gtcattacgc tgaacaacgg
    12181 ttatctgggt atggtacgcc agtggcagga aatatattac ggcggtcgag agtcggaaac
    12241 ctatttcgat tctttgcccg atttcgtcaa acttgccgag gcatacggcc atatcggcat
    12301 ccgcgtggac aagaaatctg atgtggaagg tgccttgttg gaagcattga accaaaaaga
    12361 caggctggtg tttatcgact tcctgaccga ccagaaacag aatgtgatgc ctatggtcgg
    12421 caacggcaaa ggtttggacg aaatggtact tccgccgcat atgcgtacgg acggaaaggc
    12481 gtaaggagag gcaaatgcga catatcttat ctgttctgat cgaaaacgaa tcaggtgcga
    12541 tgagccgcgt ggtcggtttg ttctctgcac gcgattacaa tatcgattct ttggcggttg
    12601 cgccgaccga agacaaaacc ctgtcacgga tgaccatcgt tacccacggc gacgagcagg
    12661 ttatcgaaca aatcaccaag caactcaata aattgattga agtgattaaa gtggtcgatt
    12721 tgaacgaaag ccgttttgtc gaacgcgaac tgatgttggt aaaagtccgt gccgccggca
    12781 aagaccgcga cgaattttta cgcttgaccg aaatctaccg gggcagcatc atcgacgtaa
    12841 ccgaccgcag ctatacgatt gaaattaccg gctcgaccga caagctggat tcctttttgg
    12901 aaacggtcgg acgcgcccaa attttggaaa ccgtacgtac aggtgcggcc ggcatcggtc
    12961 gcggtgagcg tattttgaaa atttaacgcc gtaacctttc agacggcatg gtatttgaat
    13021 gccgtctgaa aaacgaacgg caggagagat ttatgtcgaa cattaaaatt gtcgcactgg
    13081 ttaccgtcaa accggaatac acggaaacac tggcagcaca gtttaaagaa ctggtcaaag
    13141 ccagccgtgc ggaagagggc aacatcagct acaatctcca tcaggaaatc ggcaaaccga
    13201 accgttttgt tttcgtggaa aattggaaat cccaagcagc tattgacgaa cataatgcca
    13261 gcgcgcattt ccaagccttc gtccagtccg tcgacggcaa aaccgaagcg ttggaaatcg
    13321 tattgatgaa tgaagttgcc gtttaagcgg cacactctgt ttaacccgtc cgaagccgct
    13381 caacatttta aggcttcgac aaccatttac cttaaaggaa atcaaatgca agtctattac
    13441 gataaagatg ccgatctgtc cctgatcaaa ggcaaaaccg ttgccatcat cggttacggt
    13501 tcgcaaggtc atgcccatgc tgccaacctg aaagattcgg gtgtaaacgt ggtgatcggt
    13561 ctgcgccacg gctcttcttg gaaaaaagcc gaagcagccg gccatgttgt taaaaccgtt
    13621 gctgaagcga ccaaagaagc cgatgtcgtt atgctgctgc tgcctgacga aaccatgcct
    13681 gccgtctatc acgccgaagt tgcagccaat ttgaaagaag gcgcgacgct ggcatttgca
    13741 cacggcttca acgtgcacta caaccaaatc gttccgcgtg ccgacttgga cgtgattatg
    13801 gttgccccca aaggtccggg tcataccgta cgcagtgaat acaaacgcgg cggcggcgtg
    13861 ccttctctga ttgccgttta ccaagacaat tccggcaagg ccaaagacat cgccctgtct
    13921 tatgcggctg ccaacggcgg caccaaaggc ggtgtgattg aaaccacttt ccgcgaagaa
    13981 accgaaaccg atctgttcgg cgaacaagcc gtattgtgcg gtggcgtggc cgagttgatc
    14041 aaagcaggtt ttgaaaccct gaccgaagcc ggttacgcgc ctgaaatggc ttacttcgaa
    14101 tgcctgcacg aaatgaaact gatcgttgac ctgattttcg aaggcggtat tgccaatatg
    14161 aactactcca tttccaacaa tgcggagtac ggcgaatacg ttaccggccc tgaagtggtc
    14221 aatgcttcca gcaaagaagc catgcgcaat gccctgaaac gcatccaaac cggcgaatac
    14281 gcaaaaatgt ttatccaaga gggtaatgtc aactacgcgt ctatgactgc ccgccgccgt
    14341 ttgaatgccg accaccaagt tgaaaaagtc ggcgcacgac tgcgtgccat gatgccttgg
    14401 attacggcca acaaattggt tgaccaagac aaaaactgat tgttttcaaa cgggactgcc
    14461 tacacatcgt gtaggcagtt tgttatatgg ataccgtctg aacatcgtgt tcggacggta
    14521 tctgtgttgc ggatgaattt aaacaggcac agttctgtca gtcgcccaga gccgcgacca
    14581 ttaccgcttt aatcgtgtgc atacggtttt ccgcctgatc gaacacgatg ccggccggac
    14641 tttcgaatac ttcttctgta acttccacac cgttcagccc gaaggtttcg taaatccatt
    14701 cgccgacttt ggtttcgcgg ttgtggaagg cgggcaggca gtgcatgaat ttgacttgcg
    14761 gattgcccga tgccgccatc agttcgggcg taacgcggta atctttcagc aaatcgatgc
    14821 gttcctgcca gacttctttc ggctcgccca tgctgaccca tacgtcagta tgaatgaaac
    14881 cgacaccttt gacggcttca tgcgcgtttt cggtcagggt aatttttgca ccggtttctt
    14941 tggcggcggc gtgtgcggcg gcgataatgc cttcagacgg ccacaggctt tgaggtgcgc
    15001 cgatacgcac gtccatcccc aattttgccc ctaaaatcag cagggaattg cccatgttgt
    15061 aacgcgcgtc gccgacgtag gcaaacgcgg tttggttcaa aggtttgccg ctgtgttcgc
    15121 gcatagtcag tgcgtcggca agcatttgtg tgggatggaa ctcgttggtc agcccgttga
    15181 acacgggtac gcccgcatat tttgccaatt cttcgacagt ttcctgagcg aagccgcgat
    15241 attcgatggc atcgtacatt ctgcctaaga cgcgcgccgt gtctttgatg ctttctttgt
    15301 gcccgatttg gctggcggac ggttccagat aggttgcatc cgcgccttgg tcgcgtgcgg
    15361 cgacttcaaa cgcacagcgt gtgcgcgtgg atgttttttc aaaaatcagg gcgatgtttt
    15421 tccctttcat ccgctgaatc tcgcgccctg cctttttggc gtctttcaac tcggcggcaa
    15481 ggtcgaggta ggcggtgatt tcttccggcg tgaagtccaa aagtttcaga aaatggcggt
    15541 ttttcaggtt cactgtcgtt tcctttatgt gggggcgtga acgccgtatt tgcagcgttt
    15601 gcggaaagaa gggttagcgg aatgttgcaa tgccgtctga aggttgttca gacggcattg
    15661 cggtcaatcc cttactccat acggatgacg gggatcaaac attcacggtc tcggctactt
    15721 cgttgtagct gtcgatttcg ttgaagttca tatagcggta gattttatcg ccctgttcgt
    15781 tgatgatgcc gatattggct tgatattctt caacggtcgg gattttaccc agtttggagc
    15841 aaatcgccgc caactctgcc gagccgaggt aaacgaaggt gtttttaccc aagcggttcg
    15901 ggaagttgcg ggtcgaagtg gacatgacag ttgcaccttc gcgtacttgg gcttggttac
    15961 ccatacacaa cgaacagccc ggcatttcca tacgcgcgcc ggcgcggccg agtacgccgt
    16021 agtgaccttc gtcggacaac tctttcgcgt ccattttggt cggcggcgct acccacaggc
    16081 ggacggggat gtcgctcttg ccttccaaaa gtttggaggc ggcgcggaag tggccgatgt
    16141 tggtcataca agaaccgatg aacacttcgt cgattttggt gccggagcgt tcggacataa
    16201 agcatacgtc gtccgggtcg ttcgggcagg cgataatcgg ctctttgatg tcgtccatgt
    16261 tgatttcaat cacggcggcg tattcggcat ctttatccgc ttcgagcaac tcgggatttg
    16321 ccagccattt ttccatagct ttgatgcggc gttccaaagc gcgcggatct tgatagccgt
    16381 tggcaatcat atttttcatc aacacgacgt tggatttcat gtactcgata atcggctctt
    16441 tattgagctt cacggtacag ccggcggcgg agcgttcggc ggatgcgtcg gtcaattcaa
    16501 aggcttgttc cactttcaaa tcgggcaggc cttcgatttc gaggatgcgg ccggagaaga
    16561 tgtttttctt accggctttg gcaacggtca gcaaaccttg tttaatcgcg taaagcggga
    16621 tggcgttcac caaatcgcgc agggttacgc ccggttgcag cttgccgctg aaacgtacca
    16681 atacggactc gggcatatcg agcggtatta cgcccgttgc ggcggcaaaa gcgaccaagc
    16741 ctgaacctgc ggggaaggaa ataccgatgg ggaaacgggt gtggctgtcg ccgcctgtac
    16801 cgactgtatc aggcaacagc aggcggttga gccacgagtg gatgacgccg tcgcccggac
    16861 gcagggacac gccgccacgg gtagaaataa aggcgggcag ttctttatgg gtttttacat
    16921 cgacaggttt cggataggca gcggtgtggc agaaagactg catcaccata tcggcggaga
    16981 agcccaaaca agccaagtct ttcaactcgt cgcgggtcat cggcccggtc gtatcttgcg
    17041 agccgaccgt cgtcatgcgc ggttcgcagt aagtacccgg acgcacgcct tgtccttcgg
    17101 gcagaccgca ggcgcgaccg accatttttt gcgccaaggt gaaaccgact ttgctttcgg
    17161 caggcgcttg cggcaggtgg aatgcagtag aggcaggcag tttcagggct tcgcgcgctt
    17221 tggcggtcag accgcgaccg ataatcaggt tgatacggcc gccggcttgc acttcgtcca
    17281 gcaatacttg tgatttcaat tcaaactcgg caacagtctc gccgtttttc acgattttac
    17341 cttcataagg aaggatattg acgacatcgc ccattttcag cgcggaaaca tcgacttcaa
    17401 tcggcagcgc gccggagtct tcttgagtgt tgaagaaaat cggcgcgatt ttaccgccca
    17461 agcacacgcc gccgaagcgt ttgttcggca cgaacgggat gtcttcgccg gtatgccaaa
    17521 tgacggagtt ggtcgcggat ttgcgtgaag aaccggtacc gaccacgtca ccgacgtagg
    17581 caaccggatg gcctttggct ttgagttctt ccaacaattt aatcggaccg acttcgcccg
    17641 gtttgtcggg cgtgatgccg tcgcgcgggt ttttcagcat ggccagcgcg tgcagcggaa
    17701 tatcgggacg actccacgcg tcgggcgcgg gagagaggtc gtctgtattg gtttcgccgt
    17761 caactttgaa gacggtaacg gtgatttttt cgggaacttt ggcacgggag gcgaaccatt
    17821 cggcatctgc ccaagattgc aaaacttcct gcgcgtattt gttgcctttt tcggcttttt
    17881 cctgaacgtc gtggaaggaa tcgaacatca gaagcgtatg tttcaagcct ttggcggcaa
    17941 tgggcgcgag tttgtcgtcg tccaagagtt cgattaaggc gtggatattg taaccgccga
    18001 gcatcgtacc taagagttcg gtcgcatatt tgggggaaac cagcgggctg gatgcgctgc
    18061 cttcggcaac ggcagccagg aatgaggctt tgactttggc ggcatcgtcc acaccgggcg
    18121 gaacacggtg ggcaagcagc tcgaccaaga actcgccttc gcctgcgggc gggtttttca
    18181 gcagctcaac caaatcggcg gtttgctgcg cattcaaagg gagggcggga atgccgaggg
    18241 cggcgcgctc ggcggcggct ttacgatagg cttctaacat ctctttgttc ctttttccgt
    18301 ttttcttttg tcgggttgca aatgatctgc gttaaatatt gtaaacaaca tttgtaccgc
    18361 tatcatagac tagttttaac aaaaatggaa ctgttatgac aggtaat
//
LOCUS       DAXDXW010000043        18399 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXDXW010000043 DAXDXW010000000
VERSION     DAXDXW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18399
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.044"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(223..1440)
                     /locus_tag="ACFB64_001250"
     CDS             complement(223..1440)
                     /locus_tag="ACFB64_001250"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC1209607.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLATGEQVTIGLLAMALKDIGVDAKSYTGWQVSL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGNISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERKDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFDLG"
     gene            complement(1642..2370)
                     /gene="rph"
                     /locus_tag="ACFB64_001251"
     CDS             complement(1642..2370)
                     /gene="rph"
                     /locus_tag="ACFB64_001251"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC1209608.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SVDENAPPFLHGKNQGWVTAEYGMLPASTALRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPILE
                     AVAAVSAGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEGAPFSLDELG
                     KLVALAQKGIGELLRYQQNALSVA"
     gene            complement(2452..2916)
                     /locus_tag="ACFB64_001252"
     CDS             complement(2452..2916)
                     /locus_tag="ACFB64_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC1209609.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVGNTVKHLLVANKGAREMAQDL
                     VDYADENGAGLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3133..3909
                     /locus_tag="ACFB64_001253"
     CDS             3133..3909
                     /locus_tag="ACFB64_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC1209610.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALTHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKEKMRVPCVYSTENIMPPQSGAAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            3913..4044
                     /locus_tag="ACFB64_001254"
     CDS             3913..4044
                     /locus_tag="ACFB64_001254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209611.1"
                     /translation="MPSETGFRRHLNKRGNQAVGSEKPTSARLKPYSQSTPFQNICI"
     gene            4154..5008
                     /locus_tag="ACFB64_001255"
     CDS             4154..5008
                     /locus_tag="ACFB64_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC1209612.1"
                     /translation="MPPIPALSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKIVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVEITGQYLHYIAQMEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAAETKLPEVYIADLMQAWLGILSRAKHTRSHEVIQETLSVRAQMTAILRRLNEHGIC
                     RFHDLFNPEQGAAYVIVNFIALLELAKEGLVGIVQEDGFGEIRISLNHEGAHSDGIFG
                     TRGGRDVF"
     gene            5049..6257
                     /gene="pncB"
                     /locus_tag="ACFB64_001256"
     CDS             5049..6257
                     /gene="pncB"
                     /locus_tag="ACFB64_001256"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC1209613.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNVSTVY
                     PLADIREDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVYLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKG
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFGIPEPRTP"
     gene            6349..8067
                     /gene="argS"
                     /locus_tag="ACFB64_001257"
     CDS             6349..8067
                     /gene="argS"
                     /locus_tag="ACFB64_001257"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC1209614.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRHEFLAQNIHAALNDA
                     RFGVAKTAQPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFTGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVADDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGRLKADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENAKAEFIGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQAATLFSRFYEACPILKAEGASRNSRLQLAKLTGNTLKQGLDLLGI
                     DVLDVM"
     gene            complement(8003..8426)
                     /locus_tag="ACFB64_001258"
                     /pseudo
     CDS             complement(8003..8426)
                     /locus_tag="ACFB64_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257775.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8625..10043
                     /locus_tag="ACFB64_001259"
     CDS             8625..10043
                     /locus_tag="ACFB64_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC1209615.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLVPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAVKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATRL
                     SEPKSQKILFIVAESWGLPGNPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQKIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIRRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
     gene            complement(10496..11242)
                     /locus_tag="ACFB64_001260"
     CDS             complement(10496..11242)
                     /locus_tag="ACFB64_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC1209616.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAACSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTAALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(11226..11405)
                     /locus_tag="ACFB64_001261"
     CDS             complement(11226..11405)
                     /locus_tag="ACFB64_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209617.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            complement(11421..12092)
                     /locus_tag="ACFB64_001262"
     CDS             complement(11421..12092)
                     /locus_tag="ACFB64_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC1209618.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYDSEAVRTAVAVLDVLGTAG
                     DVGSDAPVRRRASAKSGHSYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFETDRYQREVAQVSAGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDSVGE
                     WLGIW"
     gene            complement(12161..12832)
                     /gene="rpiA"
                     /locus_tag="ACFB64_001263"
     CDS             complement(12161..12832)
                     /gene="rpiA"
                     /locus_tag="ACFB64_001263"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC1209619.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSGELLARYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEAVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(12909..13391)
                     /gene="ispF"
                     /locus_tag="ACFB64_001264"
     CDS             complement(12909..13391)
                     /gene="ispF"
                     /locus_tag="ACFB64_001264"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC1209620.1"
                     /translation="MTNIRIGQGYDVHQLIEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(13423..14112)
                     /gene="ispD"
                     /locus_tag="ACFB64_001265"
     CDS             complement(13423..14112)
                     /gene="ispD"
                     /locus_tag="ACFB64_001265"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC1209621.1"
                     /translation="MKRKNIALIPAAGIGVRFGADKPKQYVEIGSKTVLEHVLGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAVPVADTLKRAESGQISATVDRSG
                     LWQAQTPQLFQAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(14109..14843)
                     /gene="dnaQ"
                     /locus_tag="ACFB64_001266"
     CDS             complement(14109..14843)
                     /gene="dnaQ"
                     /locus_tag="ACFB64_001266"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC1209622.1"
                     /translation="MMTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEEKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGETEEEEPTAKPVASAEMKLGAKLKVIKADEAELAAHE
                     EYLDGLGEACIWRKEAVPSENGGENA"
     gene            complement(14999..16309)
                     /locus_tag="ACFB64_001267"
     CDS             complement(14999..16309)
                     /locus_tag="ACFB64_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC1209623.1"
                     /translation="MDAKKGGLGLVKSRRFGPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGVLPAGQMLNLGALLFILPYFLFSALSGQLGNKFDKAVLARWVKVLEIIIMAV
                     AAYGFYIRSAPLLLLCLFCMGAQSTLFGPLKYAILPDYLDDNELMMGNSLIESGTFVA
                     ILFGQILGTAVAGAPPYIVGILVLLVAVGGTAGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHNPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFGRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYSVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLAVFLIKRERRFLGAAAIRKKP"
     gene            complement(16299..16484)
                     /gene="ccoS"
                     /locus_tag="ACFB64_001268"
     CDS             complement(16299..16484)
                     /gene="ccoS"
                     /locus_tag="ACFB64_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC1209624.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKEKENDGR"
     gene            complement(16484..16783)
                     /locus_tag="ACFB64_001269"
     CDS             complement(16484..16783)
                     /locus_tag="ACFB64_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209625.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARLQSGRFAVKLEDGTGN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLIGNM"
     gene            17134..18330
                     /locus_tag="ACFB64_001270"
     CDS             17134..18330
                     /locus_tag="ACFB64_001270"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC1209626.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELEAHGLDSRIGAIGHRVVSGGELYNESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETAHFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITDMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT         4300 a         5434 c         4580 g         4085 t
ORIGIN      
        1 gaatccgttt tttgagtttc gcttaagttc aataaatcct gaaactttaa tctcgccgtg
       61 aaaatgaatt ttctatttca atttgtcata tatagttaat ttttttaggg tattttattt
      121 tttgttccaa agtcaaaata tgccgtccga acgttcgggc ggcagacaaa acggcactgc
      181 ccgataaagg cagtgccgtt gtccgtttta aaccgtgaaa catcagccca aatcaaaggc
      241 tttatgcaat accctggttg ccagttccat gtatttttcg tcaatcaata cggaaacttt
      301 gatttcggag gtggaaatca tttggatgtt gatgccctct tcggcgagcg tgcggaagat
      361 tttggcggct acgccgacgt gcgaacgcat acccaaaccg actgcggaga ctttgcacac
      421 ggtgtcgtcg ccgtcgatag aagctgcgcc gatactgtct ttacgttccg acaggatttc
      481 caaagtctgt ttgtaatcgc cgcgcggtac ggtgaaagaa aaatcggttg tgccttcgct
      541 gccgacattt tggataatca tatcgacttc gatgttggca tctgcgaccg cgcccaaaat
      601 ctgataggcg acgccgggct tatcgggcac accgcgcacg ttgatgcggg cttggttttt
      661 atcgaatgcg ataccggtta cggcagctct ttccatgttg tcgtcctctt caaaggtaat
      721 taaggtgccg ttgccgccgt cttgcaggct gctcagtacg cgcaggcgca ctttgtattt
      781 tccggcgaat tctactgaac ggatttgcaa aactttcgaa ccgaggctgg ccagttcgat
      841 catttcttca aatgtaaccg tatccatacg gcgcgcttcg ggtacgacgc gggggtcggt
      901 ggtgtaaacg ccgtctacgt cggtatagat ttggcattcg tccgctttga gggctgcggc
      961 aagcgcaacg gcggaagtgt cggaaccgcc gcgtcctagc gtggaaatat tgccttcact
     1021 gctgatgcct tggaagccgg caacgatgac gactttgccg gcggcgaggt cggcgcgcat
     1081 tttttcgtca tcaatgcttt ctatgcgggc tttggtgtgg gcggtatcgg ttttgaggga
     1141 gacctgccag cctgtgtagc ttttggcatc cacgccgata tccttcaatg ccattgccaa
     1201 aaggccgatg gttacttgtt cgccggtagc taagacgacg tccagctcgc gcggatcggg
     1261 atgctcctgc atttcgtgcg ccaacgcaac cagacggttg gtttcgccgc tcatggcgga
     1321 tacgacgacg acgatatcgt gtccttcggc gcgggcttta gcgacacgtt tggccacgtt
     1381 tttaatgcgc tcgggcgagc ctacggatgt gccgccgtat ttatgtacga ttaacgccat
     1441 gtttcatgct ttcttgtgtg ggattggtcg ggcagcatgg tttgctggaa aagcggctat
     1501 tattactatt ttttacatga agttcaagaa cggactgcgc tttcccgcct gccgtttgac
     1561 agcggtcagc gaaaaacctg ttctttcaga tttttgacaa aatgccgtct gaacggtttt
     1621 cagacggcat acggacagta attaagcaac ggacaacgca ttttgctgat aacgcagcag
     1681 ttcgcctatg cctttttgcg ccagtgcaac cagtttgccc aactcgtcca aactgaatgg
     1741 cgcgccttcc gccgttccct gtatttcgat gattttcccc gatgcggtca tgacgatgtt
     1801 cacatcactg tcgcagccgg agtcttcggg ataatccaaa tccaaaagcg gcacgccgtt
     1861 cactacgccc gctgatacgg cggcaacagc ttcaaggatg gggttttcac tcaaaatgcc
     1921 gtctgaaacc agtttgccga cggcgatttg cagcgcgaca aacgcaccgg taatcgaagc
     1981 cgtacgcgtg ccgccgtccg cctgaatcac atcgcaatca atcaggattt gtcgttcgcc
     2041 gagtttttcc atatccacga ccgcgcgcag cgaacgcccg atcaagcgtt ggatttcttg
     2101 tgtgcgtccc gactgtttgc ccgccgaagc ttcgcggcgc atacgcaaag cggttgaggc
     2161 aggcagcata ccgtattccg ccgttaccca gccttggttt ttgccgtgca gaaacggtgg
     2221 ggcattttcg tccacggaag cggtacaaat cactttggta ttaccgcatt caataaggca
     2281 cgaaccgtcc gtatgcggca ggaaatgagg ggtgattttg atatcgcgca ggctgtcggc
     2341 ggcgcgtgag gtgcggatat aatcaggcat actgccctcc cgttaaaaac agataaatta
     2401 aaaagcctta aatatgaaaa atcacattta aggccttcaa actgaaaatt tctacgcctc
     2461 ttcggctttg ctacggataa tcaaaagcgg caggtggctt tggcgcatta ccgtttcggc
     2521 gaaactgccc atcaaaaggt gcatcagccc ggtgcgcccg tgcgtgccca acaccagcag
     2581 gccggcaccg ttttcatcgg cataatcgac caaatcctgc gccatttcgc gcgcaccctt
     2641 attggcaacc agcaggtgtt tgacggtatt tcccacaccc agttcccggg cggtgcgctc
     2701 ggcggcatcc aaaacctcgt tgccttgcgc gacggcggcg gcctcgtaac tttcgtgttg
     2761 caaaaattcg ggggcgagcg ccatatattc ggcagggttg gcaacgtgta ccaaagtcag
     2821 acgcgcaccg ttgaccccgg caagctcggc ggcgtgtttc agggcattga tggacgtttc
     2881 actgccgtca acggcaacga ccagatgttt gtacatatcg tattctcctt ttgcaccgtc
     2941 tcacggtgcc gtctcttcag gtttgcgcaa aggcagtctg cgccgtttca ttatagaccc
     3001 gccgtcgggc tttatacaac agccgaacag cctgactgct ttccagtata atatgccgct
     3061 ttcgtgcagt caggcatttt ttgccggctt tcgttcactt tttgatttga cgcaatcttg
     3121 caggattcga ccatgtccga caacgctttg acctcttcgc gacgcttcgg cggcatcgcc
     3181 agactctacg gagactctgc cttgacgcac ttttcacagg cacacgtctg cgtggtcggc
     3241 gtgggcgggg tcggctcgtg ggcggtcgag gctttggcgc gcacgggcat cgggcgtttg
     3301 actttgattg atttggacaa cgttgccgaa tcgaatgtca accgccagct gcacgccctg
     3361 accggcgact tcggcaaagc aaaagttacc gccttgcgcg aacgtattac acaaatcaat
     3421 ccgcaatgcg aagtatttga aattgaagat ttcgttaccg aagacaattt gccggaatac
     3481 ttcggaaaag gttttgattt cgtcatcgac gcgatcgacc aagtgcgcgt caaagcggca
     3541 atggcggctt attttgtgga acgcaaacaa ccgtttgtcc tcagcggcgg cgcgggcgga
     3601 cagaaaaatc ccgccctgat tcaaaccgcc gatttaagcc gcgtaaccca cgacccgctg
     3661 cttgccaacc tgcgctacac cttgcggaaa cgctacggct tcagccgcga tactaaggaa
     3721 aaaatgcgcg tgccgtgcgt gtattcgacc gaaaatatca tgccgccgca atcgggcgca
     3781 gcgtgttctg ccgatgccgc gccgcagggc ttgtcatgcg ccggctatgg cgcaagtatg
     3841 ctcgttaccg cttcgttcgg actgtattgc gcacaggcgg cggtggaaca catcgcgggc
     3901 aaaaaataag caatgccgtc tgaaacagga ttcagacggc atttgaacaa acgcggcaat
     3961 caagctgtcg gttcagaaaa accaacatcg gcacgcctaa aaccgtattc acaatctact
     4021 ccttttcaaa atatttgcat ttaaaagccg ttataatgcc gtccgaacat ctgcccgacc
     4081 acattatgcg tgaatgccgg cagattgttt tcttttgtaa acttatatta aaattcactt
     4141 accaattcac gccatgccgc ccatccctgc cctatccgca ccatccgagc acactgtcgc
     4201 atgggtattc ggccaacccg ttaccgattt gccgcaggat ttgtttatcc cgcccgacgc
     4261 gttgaaaatc gtgttgggca gcttccaagg ccctttggat ctgctgctct accttatccg
     4321 caagcagaac atcgatgttc tcgatattcc gatggtggaa attaccgggc agtatctgca
     4381 ctatattgcc caaatggaag cctatcagtt tgatttggcg gcggaatatc ttttgatggc
     4441 ggcaatgctg attgaaatca aatcgcgcct gctgctgccg cgtaccgaag ccgtcgaaga
     4501 cgaagaggcc gacccgcgtg ccgagttggt gcgccgtctg cttgcctacg agcaaatgaa
     4561 actggcggcg cagggtttgg acgcgctgcc gcgtgcggga cgggatttcg cgtgggctta
     4621 cctgccgctg gaaattgcag ccgagacgaa gctgcccgag gtttacatcg ccgatttgat
     4681 gcaggcatgg ttgggcattc tttctcgggc aaaacatacg cgcagccacg aagtaatcca
     4741 agaaaccctt tccgtgcgcg cgcaaatgac ggcaatcctg cgccgtttga acgaacacgg
     4801 gatatgcagg tttcacgacc tgttcaatcc cgaacagggc gcggcttacg tgatcgtcaa
     4861 cttcatcgcc ctgttggagc ttgccaaaga aggattggtc ggaatcgtac aggaagacgg
     4921 tttcggagaa atccgaatca gcctcaatca tgagggggcg cattcagacg gcattttcgg
     4981 cacacggggc gggcgcgatg tgttctaata cgccccaagc cgccaccaga aatcgggaga
     5041 cacgccatat gaccggcatc atacattcgc tgctcgacac tgacctctac aaattcacta
     5101 tgctgcaagt agtcctgcac cagtttccgc agacgcacag cctttacgaa ttccgctgcc
     5161 gcaacgtctc gaccgtctat ccgcttgccg acatcaggga agacttggaa gccgaactcg
     5221 acgcgctctg ccggctgcgc ttcacccacg acgaactcgg ctatctgcgc tccctgcgtt
     5281 tcatcaaaag cgactttgtc gattatctcg aactcttcca gctccaacgc cgctttgtcg
     5341 aagtcggcac agacgataaa ggccgtctga acatccgtat cgaaggcccg atgatacagg
     5401 cgatgttttt tgaaatcttc atcctcgcca ttgtcaacga actttacttc cgccggctgg
     5461 aaacccctgc cgttatcgaa gaaggcgaac gccggcttca agccaaagcc gcgcgcctga
     5521 aagaaatcgc cgccgcacag aatccggacg aaccgccctt cctgatttcc gacttcggca
     5581 cgcgccgccg ctacaagctc gcgtggcagg aacacgtcat ccgcaccctg ctcgaagccg
     5641 ccccaagcat cgtgcgcggc acaagcaacg tttacctcgc caaaaaactc ggcatcaccc
     5701 ccatcggcac catggcgcac gaattcctcc aagccttcca agccctcgac gtgcgcctgc
     5761 ggaatttcca aaaggccgcg ctcgaaagct gggtgcacga ataccggggc gatttgggcg
     5821 ttgccctgac cgacgtggtc ggtatggatg ccttcctgcg cgatttcgac ctctatttcg
     5881 ccaaactctt cgacgggctg cgccacgaca gcggcgaccc ttacgtttgg ggcgacaaag
     5941 cctacgccca ctatcaaaag ctcaaaatcg acagccgcac caaaatgctg accttctccg
     6001 acgggctgga catcgaacgc tcttgggcat tgcaccaata tttcaaaggc cgcttcaaaa
     6061 ccggcttcgg catcggcacc aacctcacca acgatatggg gcatacgccc ttgaatatcg
     6121 tgttgaaact ggtcgaatgc aacgggcagt ccgtcgccaa gctctccgac tcgccgggca
     6181 aaaccatgac caacaacagc accttcctcg cctacctgcg ccaagtgttc ggcatacccg
     6241 aacctagaac gccgtaaacc ggcagaaaaa gcgtacaatt cctgtttccg ccgcataaaa
     6301 tcttttaaaa taccgcctga tttgaattta accgaaagac cgaacttcat gaacctacat
     6361 caaaccgtcg aacacgaagc cgccgccgcc tttgccgccg caggcatcgc cggcagcccc
     6421 gttgtcttgc agccgaccaa aaatgccgag cacggcgatt tccaaatcaa cggcgtgatg
     6481 ggggcggcga aaaaagccaa acaaaatccg cgcgaactgg cgcaaaaggt cgccgacgca
     6541 ttggcgggca acgccgtcat cgaaagcgcg gaagtggccg gacccggctt tatcaacctg
     6601 cgcctgcgcc acgaatttct cgctcaaaac attcatgcgg ctttgaacga cgcgcgtttc
     6661 ggcgtagcga aaactgccca accgcaaacc gtcgtcatcg actattcctc gcccaatctg
     6721 gcgaaggaaa tgcacgtcgg ccatctgcgt tccagcatca tcggcgacag tatttcgcgc
     6781 gtgttggaat ttacgggcaa caccgttatc cgtcaaaacc acgtcggcga ctggggtacg
     6841 cagttcggca tgttggtcgc ttatttggtc gagcagcaaa aagacaatgc cgcgttcgag
     6901 ctggcggatt tggagcagtt ttaccgcgcc gccaaagtgc gctttgacga agaccctgcc
     6961 tttgccgaca ccgcacgcga atacgttgtg aagctgcaag gcggcgatga aaccgtgttg
     7021 gcgttgtgga aacagtttgt cgatatttcg ctctcgcacg cccaagccgt ttacgacacg
     7081 ctgggcttga agctgcgccc cgaagatgtg gcgggcgaat cgaaatacaa cgacgatttg
     7141 cagcccgtag ccgatgattt ggttcaaaaa ggcctggcgg ttgaggacga cggcgcgaaa
     7201 gtcgtgttct tggacgagtt caaaaacaaa gagggcgaac ccgccgcatt tatcgtgcaa
     7261 aaacaaggcg gcggcttcct ttatgcctcc accgacttgg cgtgtttgcg ctaccgcgtc
     7321 ggccgtctca aagccgaccg cctgctgtac gtcgtcgacc accgccaagc cctgcacttc
     7381 gagcaactct ttaccacttc ccgcaaagca ggctatctgc ctgaaaatgc aaaagccgag
     7441 tttatcggct tcggcaccat gatgggcaaa gacggcaaac cgttcaaaac gcgcagcggc
     7501 gacaccgtaa aactggttga cctgctgacc gaagccgtcg agcgcgccac cgctttggtg
     7561 aaagaaaaaa atcccgaatt gggcgcggat gaagccgcca aaatcggcaa gaccgtcggc
     7621 atcggcgcag tcaaatacgc cgacttgagc aaaaaccgca ccagcgatta tgtgttcgac
     7681 tgggacgcca tgctctcatt tgaaggcaac accgccccct acctgcaata cgcctacacc
     7741 cgcgtgcaaa gcgtgttccg caaagccgga gaatgggacg caaccgcacc gaccgttttg
     7801 accgaaccac tggaaaaaca gcttgccgcc gagcttctga aatttgaaaa cgtgctgcaa
     7861 agcgtggcgg acaccgcgta tccgcactac ctcgccgcct acctttacca agccgccacc
     7921 ctgttcagcc gcttctacga agcttgcccg attttgaaag ccgaaggcgc aagccgcaac
     7981 agccgcctgc aactggcaaa actcaccggc aacacgctga aacaaggctt ggatttgctg
     8041 ggcatcgatg tgttggacgt aatgtaaaac cgcaccgccc gattgcggac aacagccctg
     8101 caatcaaccg gcggaccatc ctcatccgaa tctgaaaaag cggcgcgata caccgtatcc
     8161 gccgcccctc ccaaaatgcg aaacagacaa acgccaagca agcaagcaag caagcaagca
     8221 agcaagcaaa aaattataac cccccttcct gccgacgcac gcactttccg cgcggcttat
     8281 tccccttttc ccgcccctca aacccgcctt ttcttcaggc agggtttcag cccgcctcct
     8341 ttccctgttt tccttccctc aacacgcgtg cgcgcccctg ccgcaccgtg ccgcgctttt
     8401 gcgcccggac ggcatcgttc cgccatccgg ttctctgttt caaatacccc tgtttcagaa
     8461 agaaatgcag atgtttcaac acacaggacg acatataaag taccgcccta tgtgtcgtcc
     8521 tgatttggaa ggggttacac ccctcccaac cagataaagt ctgatcctgc cgccccgaag
     8581 gacggatgtc caagtggcgg ggtttcaacc gaaaaggaaa tacgatgaat attcacgccc
     8641 tgctctccaa acaatggacg ctgccgccat tcctgccgaa acggctgctg ctgtccctgc
     8701 tgatactgct ggtccccaat gcggtgtttt gggttttggc actgctgacc gccaccgccc
     8761 gcccgattgt caatttggac taccttcccg ccgcgctgct gatcgccctg ccttggcgtt
     8821 tcgtcaaaat tgccggcgta ttggcgtttt ggctggcggt tttgtttgac gggctgatga
     8881 tggtgatcca actcttccct tttatggacc tcatcggcgc catcaacctc gtccccttca
     8941 tcctgaccgc ccccgcccct tatcagataa tgaccgggct gttgctgctg tatatgctgg
     9001 cgatgccgtt tgtgttgcaa aaagccgccg tcaaaaccga cttccgacac attgccgtct
     9061 gtgccgccgt tgtggcggca gccggctatt tcaccggcca tttgagttac tacgaccggg
     9121 ggcggatggc caatatcttc ggcgcaaaca acttctatta cgccaaaagt caggcgatgc
     9181 tctacaccgt cagccagaat gccgacttta ttaccgccgg cctggtcgac cccgtcttcc
     9241 tccccttggg caatcagcag cgtgccgcca cgcggctgag tgagccgaaa tctcaaaaaa
     9301 tcctctttat cgtcgccgaa tcttgggggc tgccgggcaa tcccgagctt caaaacgcca
     9361 cttttgccaa actgctggcg caaaaagacc gtttttcggt ttgggaaagc ggcagttttc
     9421 ccttcatcgg cgcgacggtc gaaggcgaaa tgcgcgaatt gtgcgcctac ggcggtttgc
     9481 gcgggttcgc actgcgccgc gcacccgacg aaaaatttgc ccgctgcctc cccaaccgtt
     9541 tgaaacaaga aggttacgcc acctttgcga tgcacggcgc gggtagttcg ctttacgacc
     9601 gcttcagctg gtatccgagg gcgggctttc aaaaaatcaa aaccgccgaa aacctgatcg
     9661 gtaaaaaaac ctgcgccatt ttcggcggcg tgtgcgacag cgagctgttc ggcgaagtgt
     9721 cggcattttt caaaaaacac gacaagggac tgttttactg gatgacgctg accagccacg
     9781 ccgactatcc cgaatccgac attttcaacc acaggctcaa atgcaccgaa tacggcctgc
     9841 ccgccgaaac cgacctctgc cgcaatttca gcctgcacac ccaattcttc gaccaactgg
     9901 cggatttgat ccgacgcccc gaaatgaaag gcacggaagt catcatcgtc ggcgaccatc
     9961 cgccgcccgt cggcaacctc aatgaaacct tccgctacct caaacaggga cacgtcgcct
    10021 ggctgcactt caaaatcaaa taacaacaat gccgtctgaa cgcaccaaca gccttcagac
    10081 ggcattttgc agacagaccg acctcctcaa gcccactttt ttcatcatct ccgataaatt
    10141 gctttgtaag aatccaaaaa tccaaaatca caggaagtta tcaaaaaaac agaaaccatc
    10201 cgccgtcgtt cccgcgaaag cgggaatcca gacccggaaa ccgcagaaat ttatcggaaa
    10261 aaccgaaact cgaaaacaac ggacaaaatt cccgcctgcg caggaatgac ggctcataca
    10321 tcacccaaaa aaaattgaaa ccaaacagct gggattccct cttgcgtgag gctgacagat
    10381 gccgtctgaa agactttcag acggcatagg gcgttttctc tttgaattta aattttgcca
    10441 caaaaaagcc gccccaaatg aatacccggg cagctttttg ttgatatgac tccaatcagc
    10501 ggtgttgcgg attgtaacgt ttttccaaac gcaggaatat ccagcctaag aaagtcgtca
    10561 tcaacagata aatcagggcg gcggtgtaaa gcggttcttc ataaaccgaa taccggcccg
    10621 taatcgtatt ctgaacatac gccaactccg ccacagcaat gaccgacagc agcgagctgt
    10681 ctttcaagag cgtgatgaac tcgctcgcca aaggcggcag catacggcgc aatgcctgcg
    10741 gcagaatcac atagcgcatc gcctgcggat aggtcagtcc caaagaacac gccgcctcca
    10801 tctgtccttt gtctatagac tggatgcccg cgcggaaaat ctcacagata tacgcccccg
    10861 agttggcgat cagtgccaaa gaaccggcaa tcagcggccc gtatccgcga cgcagcgcga
    10921 ttgccgcctc gccgctgacc aaaatgccgt ctgaaggatg gacgaaaaac ggaaaccaca
    10981 catacgccca aatcacaatc tgcacaaaca gcggcgtacc ccggaacagg gtaacgtaca
    11041 gcagcgaaac cttacgcaac gcccacgcca gcacgcgcat cggcgcaccg gctttttcca
    11101 agtgaatcag gcgcgccaac gccaacaaca gacccaatac cgaaccgccc gccgtcgcca
    11161 cgaccgtcag ccccaaggtc gtcagtgcgc cgtaaagaaa catccagcgg tattcgtaaa
    11221 taatgtcaaa acgaaaatcc ataaaccgtc cgtatcaaaa accggcggaa ctgccgccgt
    11281 tgcaaaataa tccgccattt taccgtaaaa accgccgcct gaactttttt atcgcggcag
    11341 acggcggttg cgcgtctccg caaaaatgca gggcgcgcgg ttttcagacg gcatttgccg
    11401 ttcaaggccg tgcggtgtct ttaccaaatg cccaaccatt cgcccacgga atccatccaa
    11461 tccttattgc ccccgccgct cctgcctgcc cggcggtacg cccacggcgc ttgcggattt
    11521 ttagctttcc acaatccttt gcgttccctt tccgcctgaa tttgagcgtc ggcatagtcg
    11581 gcaaaatccg ccttatcctg ctgttcttta gcataacttt tataatgcca cgccgccccg
    11641 tcctgcacct gcatcaggtt caaatcggtt ttgccggcgg atacctgcgc cacttcgcgc
    11701 tgatagcggt cggtttcaaa cacacgtaca ctgactttcc taccctccgc cgccgcgcgc
    11761 aggttgtcgc gcgaacgtgt accgtaagcc tgtttcatct ccggtgcgtc gatatacgcc
    11821 atccgaattt tatgtttcgc gccgtcgccg tcgatgacgt gaagggtatc gccgtcatag
    11881 actttggaca ccgtgcctgt gtagctgtgg ccggatttcg ccgatgcccg tcggcgaacg
    11941 ggcgcgtcgg aacccacgtc ccctgccgtg ccgagtacgt cgagtacggc aaccgccgtc
    12001 cgcaccgcct cactgtcata tcccgtataa cccaacgcgc ccaaaagcga cagggcgacg
    12061 ggaagccatt tcatgatttt tttaatctgc atatttttca aatgccgatg ccgtctgaac
    12121 atctctgaac cggatttcag acggcatctt aacgtcagga ttacccttgg cagggataga
    12181 tgactttcgc accctcttcc gtccccaaaa tcaacacatc ggcggcatcg cgggcgaata
    12241 tgccgttttc gagtacgccg gtgattttgt tgatttcatc ttccatcgtc agcggctgat
    12301 cgatattcaa gccgtggaca tcgacgattt ggttgccgta aaacgtggtg tagccgatac
    12361 gcagttcggg ctgtccgccc atggcaagca gtttgcgcga aacaagggag cgcgcgcttt
    12421 cgacggcttc cacgggcagt gggaatttgc ccagccggga aacatatttg ctttcgtccg
    12481 caatgcagac gaatttttct gatgcgctgg cgacgatttt ttcgttgagg tgcgcgccgc
    12541 cgccgccctt aatcatttgc agggcgtggt tcacttcgtc cgcaccgtcg atatagaccg
    12601 ccaaaccgga cacttcgttc agggaaacga cgggaatgtc gtaccgggca agcagttcgc
    12661 cggatttttt ggaggttgaa accgcgcctt tgattttttt accgcttttg cccaaggctt
    12721 cgatgaagaa attgatggtc gaaccggtac cgatgccgat gtattcgttt tcgggtacga
    12781 attcgactgc tttttcggcg gcgatgcgct tgagttcgtc ttgtgtcgtc atatttttat
    12841 cctttgggaa accgtatcaa caaacagccg ccatcttaac atttttttgc acgtcctgcc
    12901 cgccgcgttc aaatccgtac cagcaatacc gccgcctgcg cctctatgcc ttccatccgc
    12961 ccgagatagc cgagtttttc gttggttttg cctttgatat tgacgcacga aatatcgata
    13021 cccaaatcgg cggcgatgtt ggcacgcatt tgcggaatgt gcggtgcgag tttgggtttc
    13081 tgtgcaatca cggtcgtatc gacattgacc gcctgccaac cctgcgcctg aacgctttga
    13141 tacgccgcac gcaaaaggac gcggctgtcc gcatctttga actctgcggc ggtgtcggga
    13201 aaatggctgc cgatgtcgcc caaacctgcc gcaccgagca gcgcgtcggt aacggcgtgc
    13261 agcagcgcgt cggcatcgga atgcccgagc agcccttttt caaatgggat ttcaactccg
    13321 ccaagtatca gctttctgcc ttcgatcaat tggtggacat cgtagccctg tccgatacgg
    13381 atattcgtca tcgtttgtgt tcctgatgtt ttgaattgaa tttcagacgg cattgagcag
    13441 cagcctgacg atgtatgcgt cctgcggctg cgtcagtttc aaattgcgcg cgtcgccctg
    13501 tatcagtagc ggacgcacac ccagtttttc cacggcggac gcttcatcgg taatgccgtc
    13561 caagttttcc gcagccaatg cgcggtgcag caaacccgct tgaaaaagct gcggcgtttg
    13621 cgcctgccaa agccccgaac ggtcgacagt tgcactgatt tgtccgcttt ctgcgcgctt
    13681 gagcgtatcg gcaacgggaa ctgccaaaat cccgccttcg gcggcgttgc ccgcctgttc
    13741 tatcaaccgc gtcagagctt cagacggcag gcagcagcgg gcggcatcgt gtaccagaat
    13801 attgtcggtt tccgccgcca aaccggtttc caacagtttt gccacaccgt tgcggacagt
    13861 ttcggcgcgg gtctgtccac cgtttttcca cacccgaacc tgtggaaatg ccgtctgaac
    13921 cttatcggca aacgtgtctt cgggcgagac gacaacgacg gtcaaatcga cggcctcatg
    13981 ccgttcaaaa atcccaagta catgttctaa aacggttttg cttccgattt cgacatattg
    14041 cttgggtttg tccgcaccga aacgcacccc gatgccggcg gcgggaatca gcgcgatatt
    14101 tttgcgtttc atgcgttttc cccgccgttt tcagacggca cggcttcctt acgccaaata
    14161 caggcttcgc ccaagccgtc aagatattct tcgtgcgccg ccagttcggc ttcatccgcc
    14221 ttaatcactt tcagtttggc accgagtttc atttcggcag atgcaacagg tttggctgtc
    14281 ggttcttcct cctcggtctc ccccatcaag tcgaattgcc gccgcgtcat cgccagatag
    14341 acttcgccca aaagttcgca gtcgatcaat gcgccgtgca ggacgcgctt gctgcggtcg
    14401 acggaaaaac ggttgcacag ggcatccaga ctggctttct gcccggggaa catttcgcgc
    14461 gccatcgcca gggtatcggt aacggtgcag ccgagttcct cgacggtcgg cagccccatc
    14521 cggcggaact ccatattgag gaagcccaca tcgaatttgg cattgtggat aatcagttcc
    14581 gcaccgcgca gaaaatcggc aatctgcctg ccgacctctg caaacggcgg cgcgtttttc
    14641 tcttccaaaa cctgtatcgt caagccgtgg acgcgtgccg cctcttcggg catatcgcgc
    14701 tcggggtgga catagaggtg caggtttttg tcggtcattt ggcggttgac catttccaga
    14761 ccggcaaact cgaccagacg gtcgccgccg tcggcataca ggccggtggt ttcggtatcg
    14821 aggatgattt ggcgtgtcat catatcggtg tctttcttct gtcttcgtaa attgattatt
    14881 ttttaagcaa tgtatttttc tgttttcatt tcaatgcaca aacccactta ttcacagtgt
    14941 gttcacaaca ttgggcaggc ggattgtgta ttttggggac aattttttca gacggcattc
    15001 aaggtttttt cctgattgcc gccgcgccta aaaaccgcct ttcgcgctta atcaaaaata
    15061 ccgccaacgg aatattgccc aaggcgacaa tcagatacag cagggaaatg ctgtcaaaca
    15121 aaaacagcaa taccgcgctc aaaacggcgg cggaaaccat aaagatgccg ttaacgatat
    15181 tgttggcggc aacggcgcgg gcgcggaaag tctcgctgct ggcggtttgc agccaggtat
    15241 agagcggaac ggagaaaaat ccgccgaaaa agccgatcag cgtcatcacc gccatcacgg
    15301 agtatgccca tccttgcgat aaaaaccaaa aaatgccgtt cagcccttca aaacggtgtc
    15361 cgtgcgtcag ccacaccaaa accaggccgc aaaccgtcaa acccaacgca ccaaccgtta
    15421 cccaagccaa catcagccgt tccctgccga acttggcaca cagtaccgaa ccggcggcaa
    15481 taccgatgga aaacaaagca agcatcaggt taaaaacatt atcgttgccg cccaaatgga
    15541 tttgggtaaa ggtcggcagt tgcgtggtat aaaccgcgcc gacaaaccaa aaccacgaga
    15601 tgccgataat ggcggtaaaa acgggattgt gccgcaccgt ttcacgcagc agggattttg
    15661 taccacggac aatattccac tcgatttggg tatcggcagc cttggcgggt acggacggca
    15721 taaacaggct gccggccgtt cctccgacgg cgaccagcaa aaccagtatc ccgacaatat
    15781 aaggcggcgc gccggcaacc gccgttccca aaatctgacc aaacaggatg gcgacaaacg
    15841 taccggattc aatcaggctg ttgcccatca tcagctcgtt gtcgtcgaga taatcgggca
    15901 gaatggcata tttaagcggg ccgaacagcg tcgattgcgc gcccatgcaa aacaggcaga
    15961 gcaagagcag cggggctgac cggatataaa acccgtatgc cgccaccgcc ataatgatga
    16021 tttccagcac cttgacccaa cgcgccaaaa cggccttgtc gaatttgtta cccaactgcc
    16081 ccgacagcgc ggaaaacagg aaatacggca aaataaacag cagtgcgccc aagttcaaca
    16141 tctgtccggc aggcagaacg ccgttttgcc ccaaaccgta aaacccgatc atcacaaaca
    16201 gcgcggtttt gaacacattg tcattgaacg cgccgagaaa ctgcgtagcg aaaagaggcc
    16261 cgaaacggcg gcttttaacc agtcccaaac cgcctttttt agcgtccatc gttttccttc
    16321 tctttttcaa tcagtttact tgtcgaatca tcatccatca aaatgcggtg cgccggtcct
    16381 tccaaatcgt caaactgtcc gtttttgccc gaccaccaga aaaaccagcc gatgacaaat
    16441 gccaaaataa tgctgatggg caccagaata aacatacttt ccatcacata ttccctatca
    16501 aatcgttcaa aacaaaagtc tgccctgaca cggtcagata ttcgttacgc aaagttccga
    16561 cgggggcttc gtcaaaaaac agctcgatac ggtctttgac cacgcgccaa tattggggaa
    16621 tttccgtctg accgaacggc gacaggacat ggttgcccgt cccatcttcc aatttaacgg
    16681 caaaacgccc gctctgcagc cgtgcttccg attcgtcgtc ggcaagaagg atgaaaaagc
    16741 ctatatgccg tcccgattgg tcatgaatac tgaaataatg cataaattcc ccacccgcct
    16801 tttttcagac ggcatcaact aaaaacaggg cgaatgtacc agtttggacg ggaaaaatgc
    16861 aaagaaattc tccctccccc agccgaaaac accggcaaac cgcatatccc ccttttttcc
    16921 gtcaaaatac ccgacttccg ccattttcac gcaaacgccc gattaagcca agcaattgca
    16981 aagatttttt gctagaatag cctgcttctt ttatcaaccg tttcagacgg ctccactact
    17041 ttcccgtcaa ggaaagcaaa gcggattcgg cacgaatccg attagtatcc gtgtccgatt
    17101 ccaatgccgt ctgaaacttt ccggagtaag aaaatgtccc aaaaattgat cttggttttg
    17161 aactgcggca gctcgtccct caaaggcgcg gtcctggata acggcagcgg cgaagtcctg
    17221 ctgagctgcc ttgccgaaaa actcaacctg cccgatgcct acattacatt caaagtaaac
    17281 ggcgaaaaac acaaagtcga tctgtccgca caccccgacc acaccggcgc ggtcgaagcc
    17341 ctgatggaag aactcgaagc ccacggcctc gacagccgca tcggcgccat cggccaccgc
    17401 gtcgtcagcg gcggcgaact gtacaacgaa tccatcctcg ttgacgacga agtcatcgcc
    17461 ggcatcgaaa aatgcatccc gctggctcct ctgcacaatc ccgcccacct cttgggcctg
    17521 cgtgccgcgc aaagcatttt caaaggcctg cccaacgtcg tcgtattcga tacctccttc
    17581 caccaaacca tgcccgaagt cgcctacaaa tacgccgttc cgcaggagtt gtatgaaaaa
    17641 tacggcctgc gccgctacgg cgcgcacggt accagctacc gcttcgttgc cgacgaaacc
    17701 gcgcacttcc tcggcaaaga caaaaaagac ctgcgtatgg tcattgccca cttgggcaac
    17761 ggcgcatcca tcaccgccgt cgccaacgga gaatcgcgcg acaccagtat gggcctgacc
    17821 ccgctggaag ggctggtaat gggtacgcgc agcggcgaca tcgatccttc cgtattcggc
    17881 ttcctcgccg aaaacgccaa tatgaccatt gcccaaatca ccgacatgct gaacaaaaaa
    17941 tccggcctgc tcggcatttc cggcctgtcc aacgactgcc gcaccattga agaagaagcc
    18001 gccaaggggc ataaaggcgc gaaattggcc ttggatatgt ttatctaccg ccttgccaaa
    18061 tacatcggca gtatggcggt tgccgcaggc ggcttggacg cactggtctt taccggcggt
    18121 atcggcgaaa actccgacat catccgcgaa cgcgtaatcg gctacttggg cttcctcggt
    18181 ctgaacatcg accaagaagc caacctgaaa gcccgcttcg gcaacgccgg cgtgattacc
    18241 actgccgaca gcaaagccgt tgccgtggtc attccgacca acgaagagct gatgattgcc
    18301 cacgataccg cccgtttgag cggtctgtaa ggttttatcc tcacgcgaac tgcctccgga
    18361 aacggaggca gtttttttat agtggattaa atttaaacc
//
LOCUS       DAXDXW010000044        18341 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXDXW010000044 DAXDXW010000000
VERSION     DAXDXW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18341
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.045"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            6..81
                     /locus_tag="ACFB64_001271"
     tRNA            6..81
                     /locus_tag="ACFB64_001271"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Trp,seq:cca)
     gene            196..474
                     /gene="secE"
                     /locus_tag="ACFB64_001272"
     CDS             196..474
                     /gene="secE"
                     /locus_tag="ACFB64_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC1209627.1"
                     /translation="MTEHTPEKKNVKVNQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            476..1012
                     /gene="nusG"
                     /locus_tag="ACFB64_001273"
     CDS             476..1012
                     /gene="nusG"
                     /locus_tag="ACFB64_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC1209628.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1113..1547
                     /gene="rplK"
                     /locus_tag="ACFB64_001274"
     CDS             1113..1547
                     /gene="rplK"
                     /locus_tag="ACFB64_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC1209629.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMESGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1547..2242
                     /gene="rplA"
                     /locus_tag="ACFB64_001275"
     CDS             1547..2242
                     /gene="rplA"
                     /locus_tag="ACFB64_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC1209630.1"
                     /translation="MAKVSKRLKVLRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDSRKSDQVIRGSVVLPKGTGKTTRVAVFTQGVNAEAAKEAGADVVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKIGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGVRVDTSSVNN"
     gene            2470..2970
                     /gene="rplJ"
                     /locus_tag="ACFB64_001276"
     CDS             2470..2970
                     /gene="rplJ"
                     /locus_tag="ACFB64_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC1209631.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFVELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3028..3399
                     /gene="rplL"
                     /locus_tag="ACFB64_001277"
     CDS             3028..3399
                     /gene="rplL"
                     /locus_tag="ACFB64_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC1209632.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEAAGAKVEIK"
     gene            3590..7768
                     /gene="rpoB"
                     /locus_tag="ACFB64_001278"
     CDS             3590..7768
                     /gene="rpoB"
                     /locus_tag="ACFB64_001278"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC1209633.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVYYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEQESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKLSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKERRKAGELREFLNKLYNGSGKKEDLDSLTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSEDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(7733..7873)
                     /locus_tag="ACFB64_001279"
     CDS             complement(7733..7873)
                     /locus_tag="ACFB64_001279"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209634.1"
                     /translation="MDKGTVLSDGISFLNIVGLPRIPAEQLPLCRLKPVSVAFQIDIQ
                     TQ"
     gene            7921..12096
                     /gene="rpoC"
                     /locus_tag="ACFB64_001280"
     CDS             7921..12096
                     /gene="rpoC"
                     /locus_tag="ACFB64_001280"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC1209635.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLNMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLDVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENMEEGVTVAKQTDDVTGLSALVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENILLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12385..13455
                     /locus_tag="ACFB64_001281"
     CDS             12385..13455
                     /locus_tag="ACFB64_001281"
                     /inference="COORDINATES: protein motif:HMM:NF020373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HFC1209636.1"
                     /translation="MKVSQSAMNKLVIAVIAVIASMGLTACAGGGSDSSMSVQPSVSE
                     QLKDNANVDAKDEKVIEYLKKSSLKDVPKELQAKVLKVKGDEYTGVRKQYAGKLGKGE
                     SVKAMLFLDGEEPFSKEQLQKMDVYVNGKKYEGSKGGELDVLPKGLSEQKIEFYGADK
                     EQNYALLKTWVYEQPYSVVRGYFGYSRKDGNPIEEDGQNPEEIPFDLYLGDIRGVATD
                     EDKLPKAGSFQYEGRAFGGNGVLSKESLDNHNGVFRYTIDFDRRKGSGSIEGMEQYGK
                     IKLEEAAIERIPYRESGSSLGLKDRVSYFGVNEGVAMLEKDNEIKKYHLGIFGEAANE
                     VAGAVSQEHKHQAVIGFGGEKK"
     gene            13695..13937
                     /locus_tag="ACFB64_001282"
     CDS             13695..13937
                     /locus_tag="ACFB64_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209637.1"
                     /translation="MKSKNIFFMGYLIASATFAEDIGVPVEPINVGSRAAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGGRSIP"
     gene            14134..14994
                     /locus_tag="ACFB64_001283"
                     /pseudo
     CDS             14134..14994
                     /locus_tag="ACFB64_001283"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
     gene            15173..15544
                     /gene="rpsL"
                     /locus_tag="ACFB64_001284"
     CDS             15173..15544
                     /gene="rpsL"
                     /locus_tag="ACFB64_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC1209638.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            15662..16132
                     /gene="rpsG"
                     /locus_tag="ACFB64_001285"
     CDS             15662..16132
                     /gene="rpsG"
                     /locus_tag="ACFB64_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC1209639.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKAAIEVFNEAIANSKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            16151..18256
                     /gene="fusA"
                     /locus_tag="ACFB64_001286"
     CDS             16151..18256
                     /gene="fusA"
                     /locus_tag="ACFB64_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC1209640.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDSFTGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGEDLAEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4952 a         3970 c         4760 g         4659 t
ORIGIN      
        1 tttagaggcc aatagctcaa ttggtagagt atcggtctcc aaaaccgagg gttgggggtt
       61 cgagaccctc ttggcctgcc aaataaaaaa ttaaccggcc ttgtgccggt taattttttt
      121 gtatttgtta tttagtaaac tctcttgcca tttacatgga ttgagaatag acagatgcta
      181 tgatggataa ataatatgac agaacatacg cctgaaaaaa agaatgttaa agtgaatcaa
      241 ctggttgttc aagataaaga atctgcatct aattccggta aggagggttt ttttgcatat
      301 ttctcaaatt cttggtccga attcaaaaag gtggtttggc ctaagcgtga agatgccgtc
      361 agaatgactg tatttgttat agtgtttgtt gctgtgcttt ctatatttat ctatgcggca
      421 gatacagcaa tttcgtggtt attttttgat gtattgctga gaagggaagg ttgagatgtc
      481 gaaaaaatgg tatgttgtac aggcgtattc ggggtttgag aagaatgtcc aacgaatatt
      541 ggaagagcga attgcccgtg aggagatggg agattatttc ggacaaattc tggtgcctgt
      601 agagaaagtt gttgatatcc gcaatggtcg taagactatt agtgaaagaa agtcatatcc
      661 tggttatgtg ttagttgaga tggaaatgac agatgactct tggcatcttg taaaaagcac
      721 tccccgtgtt tctggtttta ttggagggag ggctaataga cccacgccga ttagtcagag
      781 agaggctgaa attattttac agcaggttca gaccggcata gagaagccga aaccaaaagt
      841 tgaatttgag gttggtcaac aggttcgtgt aaatgaagga ccgtttgcgg attttaacgg
      901 ggtggttgag gaggtcaatt atgaacggaa taagttacgc gtgtctgttc agatatttgg
      961 tagagaaaca cccgttgagc tggagttcag ccaggttgag aagattaact gatttttata
     1021 cttgaaaaaa aagcaataag aggatagaat caaaaattaa cttggggagc ggaaatggtt
     1081 ccgcgtctta cccgttttta ggagtccgtt aagtggcaaa gaaaattatc ggctatatta
     1141 aactgcaaat tcctgcaggc aaagccaatc catccccccc agttggtcct gctttgggtc
     1201 aacgtggttt gaatatcatg gaattctgta aagcatttaa tgctgcaacc caaggtatgg
     1261 aatctggttt gccaattccg gttgtgatta ctgcatttgc agataaatca ttcacatttg
     1321 tgatgaaaac tcctccggct tctattctgt tgaaaaaagc cgccggtttg caaaaaggta
     1381 gttctaatcc tctgacaaac aaggtaggta aattgacccg tgcccagttg gaagaaattg
     1441 ctaaaactaa agagcctgat ttgactgctg ctgacttgga tgcggctgtt cgtactatag
     1501 caggttctgc tcgctcaatg ggcttggacg tggagggtgt tgtataatgg ctaaagtatc
     1561 taaacgcttg aaagttcttc gctcttctgt tgaagctaac aaactgtacg caatcgacga
     1621 agcaattgcc ttggttaaaa aagctgctac cgctaaattt gacgaatctg ttgacgtatc
     1681 tttcaacttg ggcgttgatt ctcgtaaatc tgaccaagtt atccgtggtt cagtcgttct
     1741 gcctaaaggt actggtaaga caacccgtgt ggctgtattt actcaaggtg taaatgcaga
     1801 agctgctaaa gaggctggtg cggatgtcgt cggtttcgaa gatttggctg ctgaaatcaa
     1861 agcaggcaat ctgaactttg atgttgttat tgcttctcct gatgcaatgc gtatcgttgg
     1921 tcaattgggt accatcttgg gtcctcgtgg tctgatgcca aaccctaaaa taggtacggt
     1981 tacccctaat gttgccgaag cggtaaaaaa tgcaaaagca ggtcaagtgc agtaccgtac
     2041 cgacaaggca ggtattgttc atgcaacgat tggtcgtgct tcgttcgctg aagctgattt
     2101 gaaagagaac tttgatgcgt tgctggacgc tatcgttaaa gccaaacctg ctgctgctaa
     2161 aggtcagtac ctgaaaaaag ttgctgtatc cagcactatg ggtttaggtg ttcgcgttga
     2221 tacatcaagc gtgaataact aatctaagaa attttcaagc aactctgttt ttgagttgct
     2281 tgaatttggg ctacttaaaa ttaagtagat gtccaagacc gtagggatcg taagatttaa
     2341 tcgtaactgc cctacgcaga cggtagtcct gaaacacatt gcaagattgc ttgtaagatg
     2401 tctttttagg ttaccgcgct ggtgggatat cgttttggta tcctgtttat aaacagtggg
     2461 aggtagacct tgagtctcaa tattgaaacc aagaaagtgg cggtcgagga aattagcgcg
     2521 gcaattgcta atgctcaaac cctcgtagtc gctgaatatc gcggtatcag tgtttccagt
     2581 atgactgagc ttcgtgcgaa tgcacgtaaa gaaggcgttt atttgcgcgt tctgaaaaat
     2641 actctggctc gtcgcgcagt gcaaggtact tcatttgtag aattggccga tcaaatggtt
     2701 ggtccgttgg tttacgctgc ttctgaagat gctgttgctg ctgctaaagt gttgcaccaa
     2761 ttcgcgaaaa aagatgacaa aattgtcgtt aaagccggtt cttacaatgg cgaagtaatg
     2821 aatgctgctc aggttgctga gttggcttct attccgagcc gcgaagagct gttgtccaaa
     2881 ctgttgttcg ttatgcaagc tcctgtatcg ggctttgcgc gcggtttggc tgctttggca
     2941 gagaaaaaag ccggcgaaga agctgcttaa tcgattttgt ttctgttaat caattatttt
     3001 ttaatacaat atttggagta aaatagcatg gctattacta aagaagacat tttggaagca
     3061 gttggttctt tgaccgtaat ggaattgaat gacctggtta aagcttttga agaaaaattc
     3121 ggtgtttctg ctgctgctgt tgcagttgca ggtcctgctg gtgccggtgc tgccgatgct
     3181 gaagaaaaaa ccgaatttga tgtcgttttg gcttctgccg gcgatcaaaa agtcggcgtg
     3241 attaaagttg tccgtgcaat tactggtttg ggtctgaaag aagctaaaga catcgttgac
     3301 ggcgcaccta aaaccattaa agagggtgtt tctaaagctg aagccgaaga catccaaaaa
     3361 caactggaag cagcaggcgc taaagtcgaa atcaaataat ttgatgcttc ttatgaaggc
     3421 tggcagtttt ctgccagcct tattttgctt cttaaaataa gcattaagta ttgtttacat
     3481 ttatttgcat agtttttatc aaatcattgc aaataaatgt aaatatcaga ttgatgcgta
     3541 ccgttgtttt cagacggcct attattgaaa attacttttc ggagtgtgta tgaactattc
     3601 gtttaccgag aaaaaacgta tccgtaagag ttttgcaaag cgggaaaatg ttttggaagt
     3661 tccttttcta ctggcaaccc aaattgattc ttatgcgaaa tttttgcagc tggaaaatgc
     3721 ttttgacaaa cgtaccgatg acggtctgca agcggcattt aattctattt tcccgattgt
     3781 gagccacaac ggttatgctc gtctggagtt tgtatattac acgctgggcg agcctttgtt
     3841 cgatatccct gaatgtcaat tgcgcggaat cacttatgct gcccctctgc gcgcgcgtat
     3901 ccgtttggtg attttggata aggaagcgtc taaaccgacg gtaaaagaag ttcgtgaaaa
     3961 cgaagtgtat atgggcgaaa ttccgttgat gaccccgagc ggttcttttg tgattaacgg
     4021 cacagagcgt gtgattgttt cccagttgca ccgttcgccc ggtgtgttct tcgagcatga
     4081 caaaggtaag acacactctt ccggcaaatt gttattctcc gcccgcatca ttccctaccg
     4141 tggttcatgg ttggattttg aatttgatcc gaaagatttg ctgtatttcc gtatcgaccg
     4201 ccgccgtaaa atgccggtaa cgattttgtt gaaggctttg ggctacaaca atgagcaaat
     4261 cttggatatt ttctacgaca aagaaacgtt ttatttgtct tcaaacggtg ttcaaaccga
     4321 tttggtcgca ggccgtctga aaggcgaaac tgccaaggtc gatatcttgg ataaagaagg
     4381 caatgtattg gttgccaaag gtaagcgcat tactgcgaaa aatatccgtg atattaccaa
     4441 tgcaggcctg acccgtttgg atgtagaaca ggaaagcctg ctgggcaaag cattggctgc
     4501 cgatttgatt gattcggaaa ccggcgaggt attggcttct gccaatgatg aaattacaga
     4561 agagttgttg gccaaatttg atattaacgg cgtaaaagaa attacgaccc tttacatcaa
     4621 tgagttggat cagggtgctt atatttccaa taccttgcgc acggatgaga ctgccggccg
     4681 gcaggcggcg cgtgttgcga tttaccgtat gatgcgtccg ggcgaaccgc ccaccgaaga
     4741 ggcggtcgag caattgttta accgcttgtt cttcagtgaa gacagctacg atttgtcccg
     4801 cgtaggccgt atgaaattta atacgcgcac atacgaacaa aaactgtccg aagcccaaca
     4861 aaactcttgg tacggccgcc tgttgaacga gacgtttgcc ggtgctgccg acaaaggcgg
     4921 ttatgtcctg agcgtcgaag atattgtcgc ctcgattgcg actttggtcg agttgcgtaa
     4981 cggccatggc gaagtggacg atatcgatca cttgggcaac cgtcgagtac ggtcggtagg
     5041 cgagctgact gaaaaccaat tccgcagcgg tttggctcgt gtggaacgtg ccgtaaaaga
     5101 acgtttgaat caggcggaat cagaaaactt gatgccgcac gacttgatta atgcgaaacc
     5161 cgtttctgcc gccatcaaag aattcttcgg ctccagccaa ttgagtcagt ttatggatca
     5221 gaccaatccc ttgtctgaag taacccataa acgccgtgta tctgcattgg gcccgggcgg
     5281 tttgacccgc gaacgtgccg gcttcgaagt gcgggacgtg catccgaccc actatggacg
     5341 tgtatgtcct attgaaacgc ctgaaggtcc gaacatcggt ttgatcaact cattgtctgt
     5401 ttacgcgcgc accaatgatt acggtttctt ggaaacgcct taccgccgcg ttattgacgg
     5461 caaagtaacc gaggaaattg attacttgtc tgccatcgaa gaaggccgct atgtgattgc
     5521 acaggcgaat gccgatttgg attcagacgg caatctgatt ggcgatttgg ttacctgtcg
     5581 tgaaaaaggc gaaaccatta tggcaacgcc cgaccgcgtc caatatatgg acgtggcaac
     5641 cggtcaggtg gtatccgttg cggcatccct gattccgttc ttggaacacg atgacgcgaa
     5701 ccgcgcattg atgggtgcca acatgcaacg tcaggcagtg ccttgtctgc gtcctgaaaa
     5761 accgatggtc ggtaccggta tcgagcgttc cgttgccgtt gactctgcta ctgcaatcgt
     5821 tgcccgccga ggcggcgtgg tcgagtatgt cgatgccaac cgcgttgtga tccgtgtcca
     5881 tgacgacgaa gcgactgccg gcgaagtggg tgtcgatatt tacaatttgg ttaaattcac
     5941 ccgttccaat caatctacca acatcaacca acgtccggct gtcaaagcag gcgatgtttt
     6001 gcaacgcggc gatttggtgg ccgacggcgc gtccaccgat ttgggcgaat tggctttggg
     6061 tcaaaatatg accatcgcct tcatgccgtg gaacggttac aactacgaag actcgattct
     6121 gatttccgaa aaagtggctg cggacgaccg ctatacttcg attcacattg aggaattgaa
     6181 tgtcgttgcc cgcgatacca agctgggtgc ggaagacatt acccgcgata ttccgaactt
     6241 gtccgagcgt atgcaaaacc gtttggacga atccggtatc gtttacatcg gtgcggaagt
     6301 agaagccggc gatgtgttgg taggcaaggt aacgcctaaa ggcgaaaccc agctgacgcc
     6361 ggaagaaaaa ctgctgcgcg ccatcttcgg cgaaaaagcg tctgacgtaa aagatacttc
     6421 attgcgtatg cctaccggta tgagcggtac ggttatcgac gttcaagtct ttacccgcga
     6481 aggcatccaa cgcgacaaac gcgcccaatc cattatcgat tccgaattga aacgctaccg
     6541 tttggatttg aacgaccaat tgcgtatttt cgacaacgac gcattcgacc gtatcgagcg
     6601 tatgattgtc ggtcagaaag ccaacggcgg tccgatgaag ctggccaaag gcagcgaaat
     6661 cacgaccgaa tatctggcgg gtctgccgag caggcacgat tggtttgata tccgtttgac
     6721 cgatgaagat ttggccaagc agttggaact gattaaattg agcctgcaac aaaaacgcga
     6781 agaagcggat gagttgtatg agatcaagaa gaaaaaactg acccaaggcg acgagctgca
     6841 acccggcgta caaaaaatgg tgaaagtctt tatcgccatc aaacgccgtc tgcaagccgg
     6901 tgacaaaatg gcgggccgcc acggtaataa aggtgtggta tctcgcattc tgcctgtgga
     6961 agacatgccg tacatggcgg acggccgtcc tgtggacatc gtactgaacc cgttgggcgt
     7021 accttcccgt atgaacatcg gtcagatttt ggaagttcac ttgggttggg cggcaaaagg
     7081 tatcggcgag cgcatcgacc gtatgctgaa agagcgacgc aaagccggcg agctgcgcga
     7141 gttcttgaac aaactctaca acggcagcgg taagaaagaa gatttggaca gcctgactga
     7201 tgaagaaatc atcgaattgg cttccaatct gcgtaaaggt gcatctttcg cctctcctgt
     7261 attcgacggt gcgaaagagt ctgaaatccg cgaaatgttg aacttggctt acccaagcga
     7321 agatcctgag gttgagaaac tgggcttcaa cgacagtaaa actcaaatca cgctgtatga
     7381 cggccgttca ggcgaagcat ttgaccgcaa ggttacagtc ggtgtgatgc actatctgaa
     7441 actgcaccac ttggttgacg aaaaaatgca cgcgcgttct accggcccgt acagtctggt
     7501 tactcaacaa cctctgggcg gtaaagctca gtttggcggc caacgtttcg gtgagatgga
     7561 ggtttgggca ttggaagcat acggcgcggc atacacgctg caagagatgc tgactgtgaa
     7621 gtctgacgac gtgaacggcc gtaccaaaat gtacgaaaac atcgtcaaag gcgaacacaa
     7681 aatcgatgcc ggtatgcccg agtccttcaa cgtattggtc aaagagattc gctcactggg
     7741 tttggatatc gatttggaac gctactgata cgggtttcag acggcataag gggagctgtt
     7801 ctgcaggtat gcggggcagc ccgacaatgt ttaaaaacga aatgccgtct gaaagcactg
     7861 tacctttatc catatcgaaa atccgccacg cggtaaaaat atttccttca aggagcagaa
     7921 atgaatttgt tgaacttatt taatccgttg caaaccgcag gtatggaaga agagtttgat
     7981 gccatcaaaa tcggtattgc ctctcccgaa accatccgct catggtctta tggcgaagtt
     8041 aaaaaacctg aaaccatcaa ctaccgtacg ttcaaacccg agcgcgacgg cttgttctgc
     8101 gccaaaatct ttggcccggt caaagactac gaatgcttgt gcggaaaata caaacgattg
     8161 aaatttaaag gcgtaacctg tgaaaaatgt ggcgtagaag ttaccctgtc caaagtgcgc
     8221 cgcgaacgca tgggccatat cgaattggct gcacccgttg cccatatttg gttcttgaaa
     8281 tccctgcctt cccgcttggg tatggtattg aacatgacgt tgcgtgatat cgaacgcgta
     8341 ttgtactttg aagcattcgt ggtgaccgat cccggcatga ctccgttgca acgtcgtcaa
     8401 ttgctgactg aagacgatta ctacaacaaa ctggacgaat acggcgacga cttcgatgcc
     8461 aaaatgggtg cggaaggtat ccgcgaattg ttgcgaacct tggatgtagc aggcgaaatc
     8521 gaaatcctgc gccaagagct tgaatcgacc ggttctgaca ccaaaatcaa aaaaatcgct
     8581 aaacgcttga aagtattgga agccttccat cgttccggta tgaagctgga gtggatgatt
     8641 atggatgtgc tgccggtatt gccgcctgat ttgcgtccgt tggttccgtt ggacggcggc
     8701 cgttttgcca cttccgattt gaacgatttg taccgccgcg tcatcaaccg caacaaccgt
     8761 ctgaaacgcc tgctggaact gcatgcgcct gacatcatcg tccgcaacga aaaacgtatg
     8821 ttgcaagaag cggttgactc gctgttggat aacggccgtc gcggtaaagc catgaccgga
     8881 gccaacaaac gtccgctgaa atcgttggca gatatgatta aaggtaaagg cggccgcttc
     8941 cgtcaaaacc tgttgggcaa acgtgtggac tactccggcc gttccgtgat taccgtaggc
     9001 ccatacctgc gtctgcacca atgcggtttg ccgaaaaaaa tggcgttgga actgttcaaa
     9061 ccatttattt tccacaaatt ggaaaaacaa ggtttggcgt ctaccgttaa agctgcgaaa
     9121 aaattggtag aacaagaagt acctgaagta tgggatatct tggaagaagt catccgcgaa
     9181 catccgatta tgttgaaccg tgcgccgacc ctgcaccgtt tgggtattca agcgttcgag
     9241 cctattctga tcgaaggtaa agcgattcag ttgcacccat tggtgtgtgc cgcatttaat
     9301 gccgactttg acggtgacca aatggcagta cacgttccat tgagcttgga agcgcaaatg
     9361 gaagcacgca cgctgatgct ggcttcaaac aacgtattgt ctcctgccaa cggcgaaccg
     9421 attatcgtac cttcccaaga catcgtattg ggtctgtact acatgacccg cgaccgtatc
     9481 aatgccaaag gcgagggcag cctgtttgcc gatgtgaaag aagtgcatcg tgcataccat
     9541 accaaacagg tcgagctggg tacaaaaata accgtacgtc tgcgcgaatg ggtgaaaaac
     9601 gaagcaggtg agtttgagcc tgtcgttaac cgttacgaaa caaccgtcgg ccgtgcattg
     9661 ttgagcgaaa tcctgccgaa aggcctgccg tttgaatatg tcaacaaagc gttgaagaaa
     9721 aaagaaattt ccaagctgat taacgcatcg ttccgcctgt gcggcttgcg cgatacggtt
     9781 atcttcgccg accacctgat gtacaccggt ttcggatttg cggcaaaagg cggtatttcc
     9841 attgccgttg acgatatgga aattccaaaa gaaaaagcgg ccttgctggc tgaagccaat
     9901 gccgaagtta aagaaatcga agaccaatac cgtcaaggtt tggttaccaa cggcgaacgc
     9961 tacaacaaag tggtggatat ttggggtcgt gccggcgata agattgctaa agcgatgatg
    10021 gacaacctgt ccaaacaaaa agttatcgac cgtgacggca acgaagtcga tcaagagtca
    10081 ttcaactcca tttatatgat ggcggactcc ggtgcccgtg gttctgcggc tcagattaaa
    10141 cagttgtccg gtatgcgcgg tttgatggcg aaacctgacg gatcgattat tgaaacgccg
    10201 attacctcaa acttccgcga aggtctgacc gtattgcaat actttattgc gacccacggt
    10261 gcgcgtaagg gtttggcgga taccgcgttg aaaaccgcga actccggtta cctgacccgt
    10321 cgtctggtag acgtaactca agacttggtc gttgttgaag acgattgcgg tacttcagac
    10381 ggctttgtca tgaaggcagt ggtacaaggc ggtgatgtga ttgaagcatt gcgcgatcgt
    10441 attttgggtc gtgttaccgc gtctgacgtt gtcgatccgt caagcggcga gactttggtt
    10501 gaagccggta cgttgttgac tgaaaaactg gtggatatga ttgaccaatc cggtgtcgat
    10561 gaagtcaaag tccgtactcc gattacttgt aaaacccgcc acggtctgtg cgcgcactgt
    10621 tacggccgcg acttggcgcg cggcaaactg gtcaatgccg gcgaggcagt cggcgtgatt
    10681 gcggctcagt ctattggtga accgggtacc cagttgacca tgcgtacgtt ccacatcggt
    10741 ggtgcggcat cccgtgcggc agcagccagc caagtcgaag ccaaatccaa cggtacggca
    10801 cgtttcagca gccaaatgcg ttatgttgcc aacaacaaag gcgagttggt tgtcatcggc
    10861 cgttcttgcg aagtcgtgat tcacgatgac atcggccgtg aacgcgaacg ccacaaagta
    10921 ccttacggtg ccatcctgct ggtacaagac ggtatggcca ttaaagccgg tcaaaccttg
    10981 gcaacttggg atccgcatac ccgtccgatg attaccgaac atgcaggtat ggtgaaattc
    11041 gagaacatgg aagagggcgt taccgttgca aaacaaaccg atgatgtaac cggtttgtcc
    11101 gctttggtag tgattgacgg taaacgccgt tcctctagtg cttccaaact gctgcgtccg
    11161 actgtgaaac tcttggacga aaacggcgtg gaaatctgta ttcccggtac gtccactccg
    11221 gtttcgatgg cattccccgt tggtgcggtg attaccgtac gcgaaggtca ggaaatcggt
    11281 aaaggcgacg tattggcgcg tattccgcaa gcctcttcca aaacccgcga cattaccggc
    11341 ggtctgccgc gcgttgccga gctgtttgaa gcacgcgtgc cgaaagatgc cggtatgctg
    11401 gcggaaatta ccggtaccgt ttccttcggt aaggagacca aaggcaaaca acgtctgatt
    11461 atcactgacg tggacggtgt agcatacgag actttgattt ccaaagagaa acaaattctg
    11521 gtacatgacg gtcaagtggt aaaccgaggc gaaaccattg tggacggcgc ggtcgatccg
    11581 cacgatattc tgcgtctgca aggtatcgaa gcactggcac gctacattgt ccaagaggtg
    11641 caagaggttt accgcctgca aggtgtgaag atttccgata aacacatcga agtcatcatc
    11701 cgtcaaatgt tgcgccgtgt gaacattgcg gatgccggcg aaaccgggtt tattaccgga
    11761 gagcaggtcg aacgcggcga tgtgatggcg gccaatgaaa aagctttgga agaaggcaaa
    11821 gagccggcac gttacgaaaa catattgctg ggtattacca aagcctccct gtccaccgac
    11881 agcttcattt ctgccgcatc gttccaagaa accacccgtg tgttgaccga agctgccatc
    11941 atgggcaaac aagacgaact gcgcggtctg aaagagaatg tcatcgtcgg tcgcctgatt
    12001 cctgccggta ccggtttgac ttatcaccgc agccgtcatc agcaatggca aggggtagag
    12061 caggagactg ctgaaaccca agtaacggat gaataatctt cggcgcgttc attcaataaa
    12121 aaaccgcaag ccttgagctt gcggtttttc tttgtccgat taaggcaaaa acaagcgttt
    12181 tcgtcatttt gaggcgtgtg gatataagcc cgataagtgt cccatatctt cgtgccgtca
    12241 aattatgcat cgaatagaac gagttaaatc cgaaggttat tgtgttaaat attgcaaaaa
    12301 tactttaaaa atttcgcacg gtgggggggt agaattccca gacttaaagc ttaagttttt
    12361 ttaacaaagt ttgggggagt ctaaatgaaa gtatctcaat cagcaatgaa taaacttgtt
    12421 attgctgtta ttgctgttat tgcttctatg gggctaacag cgtgcgcggg tggcggttcc
    12481 gattcatcta tgtcggttca accaagcgtt tcggaacaac tgaaagacaa tgcaaatgtt
    12541 gatgcgaaag atgaaaaagt tattgaatat ctgaagaaat catcattaaa agatgtacca
    12601 aaagaattgc aagccaaagt attaaaggtt aaaggtgatg aatatacagg agtgcgaaaa
    12661 caatatgcag gtaagctcgg taagggtgaa tctgtcaagg caatgctatt tctagacggc
    12721 gaagagccgt ttagtaagga acaacttcag aagatggatg tatatgttaa tggtaaaaaa
    12781 tacgaaggta gtaagggggg ggaactagat gttctcccca aaggtttgag tgagcaaaag
    12841 attgagtttt acggtgcgga taaagaacag aattatgcgc tattaaaaac ttgggtttat
    12901 gagcaaccgt attctgtggt tagaggctat tttggctaca gtcgaaaaga cggtaatcct
    12961 atagaagagg atggacaaaa tcctgaagag attccttttg acttgtatct gggcgacatt
    13021 agaggcgtgg caacagatga agacaaatta cccaaggctg ggagctttca atatgaaggt
    13081 cgggcatttg gcggtaatgg ggttttatcc aaagaatcgt tagataacca taatggcgta
    13141 tttagataca caattgattt tgatcgtaga aaaggctctg gctcaattga aggaatggaa
    13201 caatatggca agatcaaatt agaagaagct gctattgaga gaatccctta tcgagagtcc
    13261 ggctccagct tggggttgaa agaccgggtt tcttatttcg gcgtgaacga aggggtggct
    13321 atgttagaga aggataatga gattaaaaag tatcatttgg gtattttcgg cgaagcggct
    13381 aatgaagtag caggtgctgt aagccaagag cataaacatc aagctgttat cggtttcggc
    13441 ggcgagaaga aatagagaaa gcactatttc tatttagtgc cgtttgtccg ggcaagatgc
    13501 cgtctgaagt ttcagacggc attttcttca aaaaacagtc cgccaaatcg aatactgcca
    13561 agcggcaatg caatacctca aaacagcaat aatttctttt aaaacagttg attcttactg
    13621 aacataaatg gccgggcaat acgaaaagaa aaatggcgtt gctgggcatt ttatttgaat
    13681 ttgttatgaa attgatgaaa tctaaaaata tcttttttat gggataccta atcgcttcgg
    13741 cgacgtttgc cgaggatatc ggcgtgcctg tcgaaccgat taacgtcggt agtcgggctg
    13801 cgatgccgtc tgaaggggaa agcctcgccc tcctgccgtt tgcagaggat gtgccgccgg
    13861 ttcgcgatgc aatgccgtct gaagttccta aaagcgcggc aggcggcgat gttcgaggcg
    13921 gccgatctat accgtgagtt gttgtcggaa agacccgatc ttgtttatcc ccgttttgat
    13981 ttgggcgtga tgctgtttga agacaaacaa tatcgtgagg cattggttca gttgcaccgg
    14041 gccgaggagg tgttgccgcc cgatatgcgg cagcttgcac gggaatatat ccgtcaggcg
    14101 gaagcggtgc aggcgtggca tccttcgttc aatatgaact atgaacaaac agataatgta
    14161 aacaatgcgt ccctttcacg ggacatcgtc atcaacggcc ggaaatggat aaaatcggaa
    14221 gacagcctgc ccaaacgcgc caacgggata aggtatgaat tgggtattga ccggatgttt
    14281 aacatggcag gcaatcactt tgcccgctta ggcatcagcg gttccggtgt gcattattgg
    14341 aatgcccggg attttagcga acaggcgttt catgccgaag taggctaccg ttatcggaat
    14401 agccgtctag agtggggatt ccggcccttt gtcaaacaaa accggctggg caataaccgt
    14461 tatacggcaa atacgggtat tgcgttggat tacagccgcc gtctgaatga aaaatggcgc
    14521 agcacccaat ctttccagta tggccgcaaa caataccatg acgaatattt ggcaaaacgt
    14581 tacaacagta aaacgatttc ggtgagcggt acgttcagct attacgctat gtcggcttgg
    14641 cagttgtacg ggggtattag cggtatgttt gataatactg tggagaaaga gcaggcttca
    14701 cggcgttatg gtgtaagttt gggtacggtg aaaatattag acggcggtct tggactgaag
    14761 ttgggtgcgg gttacacgaa acggatattt aaagcccctg caacattaat ttacaatttt
    14821 actcggcgtg atgattaaca tcggatgagc gcggctttat ggcataaaaa actgtcgtgg
    14881 aaaggattta caccccaaat aaatttccgt tacaacaaga acaacagcaa tatgcccgcc
    14941 ttttattcgc acagcggcaa gggatggttt gtcagtatgg aaaaaacgta ttgacagtat
    15001 tttctccagc cgtccgactg attgtgaggg atgtcggtaa atatttatcg gcaaacaaga
    15061 aaatcatctt ttttcttgtc gttatgcttg actgtctgct tgcaataaaa atataattcc
    15121 actcttgccg acatggtgtc ggcaagtatt taactcaaca ggacgagaaa atatgccaac
    15181 tatcaaccaa ttggtacgca aaggccgtca aaagcccgtg tacgtaaaca aagtgcccgc
    15241 actggaagcc tgcccgcaaa aacgcggcgt gtgcacccgt gtatacacga ctacccctaa
    15301 aaaacctaac tctgcattgc gtaaagtatg taaagtccgc ctgaccaacg gttttgaagt
    15361 catttcatat atcggcggtg aaggccacaa cctgcaagag cacagcgtcg tactgattcg
    15421 cggcggccgt gtaaaagact tgccgggtgt acgttaccac actgtacgcg gttctttgga
    15481 tactgcaggt gttaaagacc gcaaacaagc ccgttctaaa tacggtgcta agcgtcctaa
    15541 ataattaccg ggactcaaat aggcacgtcg gccgcctaag ctgaacaacg gccgagtaag
    15601 tgaatactca attgggtatt catgggaata gacccgactg aatagattaa aggaaattaa
    15661 aatgccaaga cgtagagaag tccccaagcg cgacgtactg ccagatccta aattcggtag
    15721 cgtcgagttg accaaattca tgaacgtatt gatgattgac ggtaaaaaat ccgttgccga
    15781 gcgtatcgtt tacggtgcgt tggaacagat tgagaaaaaa accggcaaag cagcaatcga
    15841 agtatttaac gaagccattg caaactccaa acctatcgtg gaagtgaaaa gccgccgtgt
    15901 aggtggtgca aactaccaag ttcctgttga agttcgtcct tcacgccgtc tggctttggc
    15961 aatgcgttgg gttcgcgacg cggcccgcaa acgtggtgag aaatccatgg atctgcgttt
    16021 ggcaggcgag ttgattgatg cgtccgaagg ccgtggcggt gcgttgaaaa aacgtgaaga
    16081 agtacaccgt atggctgaag ccaacaaagc attctctcac ttccgtttct aattttgaaa
    16141 ggctaataaa atggctcgta agaccccgat cagcctgtac cgcaacatcg gtatttccgc
    16201 ccatatcgat gcgggtaaaa ccacgacgac agaacgtatt ttgttctata ccggtttgac
    16261 ccacaagctg ggcgaagtgc atgacggtgc ggctactacc gactacatgg aacaagagca
    16321 agagcgcggt attaccatta cctccgctgc cgttacttcc tactggtccg gtatggcgaa
    16381 acaattcccc gagcaccgct tcaacatcat cgacaccccg gggcacgttg actttaccgt
    16441 agaggtagag cgttctatgc gtgtattgga cggcgcggta atggtttact gtgcggtggg
    16501 cggtgttcaa ccgcaatctg aaaccgtatg gcggcaagcc aacaaatacc aagttccgcg
    16561 cttggcgttt gtcaataaaa tggaccgcca aggtgccaac ttcttccgcg ttgtcgagca
    16621 aatgaaaacc cgtttgcgcg caaaccccgt acctatcgtc attccggtag gcgcggaaga
    16681 cagtttcacc ggtgttgtcg atttgctgaa aatgaaatct atcatctgga atgaagccga
    16741 taaaggtaca acctttacct atggcgatat tcctgccgaa ttggtcgaaa ctgccgaaga
    16801 atggcgtcaa aatatgattg aagccgcagc cgaagccagc gaagaactga tggacaaata
    16861 cttgggcggt gaagatctgg ccgaagaaga aatcgtaggc gcgttgcgtc aacgtacttt
    16921 ggcaggcgaa attcagccta tgctgtgcgg ttctgcattt aaaaacaaag gtgttcaacg
    16981 tatgttggac gcagttgtag aattgctgcc agctcctacc gatattcctc cggttcaagg
    17041 tgttaatcct aacactgaag aagccgacag ccgtcaagcc agcgatgaag agaaattctc
    17101 tgcattggca ttcaaaatgt tgaacgacaa atacgtcggt cagctgacct ttatccgcgt
    17161 ttactcaggc gtagtaaaat ccggcgatac cgtactgaat tctgtaaaag gcactcgcga
    17221 acgtatcggt cgtttggtgc aaatgactgc cgcagaccgt actgaaatcg aagaagtacg
    17281 cgctggcgac atcgcagccg ctatcggtct gaaagacgtt actaccggtg aaaccttgtg
    17341 tgcggaaagc gcgccgatta tcttggaacg tatggaattc cccgagccgg taatccatat
    17401 tgccgttgag ccgaaaacca aagccgacca agagaaaatg ggtatcgccc tgaaccgctt
    17461 ggctaaagaa gacccttctt tccgcgttcg tacagacgaa gaatccggtc aaaccattat
    17521 ttccggtatg ggtgagctgc acttggaaat tattgttgac cgtatgaaac gcgaattcgg
    17581 tgtggaagca aatatcggtg cacctcaagt ggcttaccgt gaaactatcc gcaaagccgt
    17641 taaagctgaa tacaaacatg caaaacaatc cggtggtaaa ggtcaatacg gtcacgttgt
    17701 gattgaaatg gaacctatgg aaccgggtgg tgaaggttac gagtttatcg atgaaattaa
    17761 aggtggtgtg attcctcgcg aatttattcc gtctgtcgat aaaggtatcc gcgatacgtt
    17821 gcctaacggt atcgttgccg gctatcctgt agttgacgta cgtatccgtc tggtattcgg
    17881 ttcttaccat gatgtcgact cttcccaatt ggcatttgaa ttggctgctt ctcaagcgtt
    17941 taaagaaggt atgcgtcaag catctcctgc cctgcttgag ccgattatgg cagttgaagt
    18001 ggaaactccg gaagaataca tgggcgacgt aatgggcgac ttgaaccgcc gtcgcggtgt
    18061 tgtattgggt atggatgatg acggtatcgg cggtaaaaaa gtccgtgccg aagtacctct
    18121 ggcagaaatg ttcggttact cgaccgacct gcgttctgca acccaaggcc gcgctactta
    18181 ctctatggag ttcaagaaat attctgaagc tcctgcccac atagctgctg ctgtaactga
    18241 agcccgtaaa ggctaatcag gcaaataggc cgtctgaaag gctgaaatga tttttcagat
    18301 ggcattgttc tttaatcgat ctttaatgta aaggaattag c
//
LOCUS       DAXDXW010000045        18202 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXDXW010000045 DAXDXW010000000
VERSION     DAXDXW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18202
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.046"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            275..973
                     /locus_tag="ACFB64_001287"
     CDS             275..973
                     /locus_tag="ACFB64_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC1209641.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCARLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAAESKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARAGKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(1030..2034)
                     /gene="amgK"
                     /locus_tag="ACFB64_001288"
     CDS             complement(1030..2034)
                     /gene="amgK"
                     /locus_tag="ACFB64_001288"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC1209642.1"
                     /translation="MQRQTELKNWLQTVYPERDFDLSFAAADADFRRYFRAAFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVGLQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTADTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPAEFDEFYRRFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            2104..4509
                     /locus_tag="ACFB64_001289"
     CDS             2104..4509
                     /locus_tag="ACFB64_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC1209643.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAADTVAAEEADGRVAEGGAQGA
                     SESAQASDLTLGSTCLFCSNESGSPERTEAAVQGSGEASVPEDYTRIVADRMEGQSQV
                     KVRAEGSVIIERDGAVLNTDWADYDQSGDTVTVGDRFALQQDGTLIRGETLTYNLDQQ
                     TGEAHNVRMETEQGGRRLQSVSRTAEMLGEGRYKLTETQFNTCSAGDAGWYVKAASVE
                     ADRGKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSVSAGSDGVSLSVPY
                     YFNLAPNFDATFAPGIIGERGATFDGQIRYLRPDYSGQTDLTWLPHDKKSGRNNRYQA
                     KWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGGEEIAGNVNLNRRVWLDYGGRAAGGS
                     LNAGLSVQKYQTLANQSGYKDEPYAIMPRLSADWHKNAGRAQIGVSAQFTRFSHDGRQ
                     DGSRLVVYPGIKWDFSNSWGYVRPKLGLHATYYSLDSFGGKASRSVGRVLPVVNIDGG
                     TTFERNTRLFGGGVVQTIEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYYG
                     NDRINAANSLSTAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKNPRSRSD
                     WVAFASGGIGGRFTLDSSIHYNQNDKRAEHYAVGAGYRPAPGKVLNARYKYGRNEKIY
                     LQADGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEMLAGAEYKSSCGCW
                     GAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPAGRMDVAVPGYIPAHSLSAGRN
                     KRP"
     gene            4611..5612
                     /locus_tag="ACFB64_001290"
     CDS             4611..5612
                     /locus_tag="ACFB64_001290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC1209644.1"
                     /translation="MMKIKALMIAAALLAAADVHAAPQKAKTAPAKAVKAAATAQKEA
                     APAQQQGGIRFSDGIAAVADNEVITNRRLAEAVAEAKATLPEDAQISESELSRQVLMQ
                     LVNQSLIVQAGKRRNIQASEAEIDAVVAQNPALKNLSPTQRRELADNIIAEKVRQQAV
                     MQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSENAAVGAESTIRK
                     IYDQARNGTDFAGLARRYSQDASAGNGGDLGWFADGVMVPAFEEAVHALKPGQVGAPV
                     RTQFGWHIIKLNEVRDAGTPEERIRNSVRQYIFQQKAGQATVNLLRDLHSGAYVDIR"
     gene            complement(6092..7972)
                     /locus_tag="ACFB64_001291"
     CDS             complement(6092..7972)
                     /locus_tag="ACFB64_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HFC1209645.1"
                     /translation="MNIFYEESGQFKVAVVVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPVYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLRTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLSLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVRQGGGMEQIIMQRLSTVYFPGGKITMLPENWITAFSLDAGAYRPAVSIYFDVDGE
                     FNVGEPTCKIEAVNIAANLRIQAIEPHFNAETGLDQAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGNVSVVRRERGSPIDTLVSEMMILANSTWAQMLD
                     ENGLPGIFRVQPAGKVRMSTQSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQKSDAALFAALRDFDTAYAAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLTTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAAAPGGKTAGN
                     AV"
     gene            8128..8760
                     /locus_tag="ACFB64_001292"
     CDS             8128..8760
                     /locus_tag="ACFB64_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC1209646.1"
                     /translation="MENVNRVPEQARYDAERRQADEALADVFPAVSIFGSARTPQDHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIALPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPVVLVG
                     KAFWSGLAEWINAQLLARGMISEGAASLFSISDDEDEIIAYLSEHGLQTA"
     gene            8832..10202
                     /gene="purB"
                     /locus_tag="ACFB64_001293"
     CDS             8832..10202
                     /gene="purB"
                     /locus_tag="ACFB64_001293"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC1209647.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAAPEIAEVPPFSAETLAEIDKVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEARETVILPKLAEIIGKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKILQAQEFLGKINGAVGNYNAHMA
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRVNTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDVTWELLAEPIQTV
                     MRRYGVANPYEKLKDLTHGKDGITPEVLKLFIESLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            10282..12150
                     /locus_tag="ACFB64_001294"
     CDS             10282..12150
                     /locus_tag="ACFB64_001294"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC1209648.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITNIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTIELSTVFLSNIYLGFRLGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCYKK
                     TKSLRVLRNISIILFLILTASSFLPAGFYTDILNQPNTYYLSTLRFPELLVGSLLAVY
                     GQTQNGRRQTENGKRQLLSLLCFGALLVCLFVIDKHDPFIPGITLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYSRGILKQEHLRPLPG
                     APLAAENNFPETVLILGDSHAGHLRGFLDYVGSREGWKAKILSLDPECLVWVDAKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFKARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAINQYLRPIRAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPKNTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHERLLKHSRGGALQ"
     gene            complement(12286..12900)
                     /locus_tag="ACFB64_001295"
     CDS             complement(12286..12900)
                     /locus_tag="ACFB64_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFC1209649.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSGSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGKTEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGI"
     gene            complement(12936..15086)
                     /gene="dinG"
                     /locus_tag="ACFB64_001296"
     CDS             complement(12936..15086)
                     /gene="dinG"
                     /locus_tag="ACFB64_001296"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC1209650.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKAACPNRP
                     ECPFYLARDMLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGLSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALIDRLTGEFGLFIARIEQISAVWDLLSTVSIEGEEPLAKWIARRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGSFNLILRQTGLLWLPETTT
                     LALKSPFDFEKQGELYIPSIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALHLPGDYLPLLLVQGELPKAVLLQKHHQAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            complement(15267..16565)
                     /locus_tag="ACFB64_001297"
     CDS             complement(15267..16565)
                     /locus_tag="ACFB64_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC1209651.1"
                     /translation="MPSETALLLYADTRAAHTLVWFRQNLRIRDNAALCAAVAEGSPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLARRGIPLYTAASPAGLVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRSVFGKTDLTPDNGTAHTDFNRYRE
                     VWLDRFSKQPPAGSDLFAAYRQPFPENLPAPPPAALSDGIFLPQNGGETAAWRQWRRF
                     LEQAASYSVLKDFPSRKNTSLMGAYLSVGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHADDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPARPVAGV"
     gene            complement(16555..17466)
                     /locus_tag="ACFB64_001298"
     CDS             complement(16555..17466)
                     /locus_tag="ACFB64_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC1209652.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHHNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGPLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            17574..17972
                     /locus_tag="ACFB64_001299"
     CDS             17574..17972
                     /locus_tag="ACFB64_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC1209653.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            18021..>18202
                     /locus_tag="ACFB64_001300"
     CDS             18021..>18202
                     /locus_tag="ACFB64_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC1209654.1"
                     /translation="MSRQSLQQAAESRRSVYSLNKNLPVGKDEIVQIVEHAVLHTPFS
                     FNSQSARVVVLFGEEH"
BASE COUNT         3894 a         4976 c         5240 g         4092 t
ORIGIN      
        1 aaggggtgta acccttccaa atcagggcaa cgcatagggc ggtgctttat gtgccgtcct
       61 gtgtgttgaa acatcattgt tccccaacag ccgtatccgt ccggggcggc gcgtattgta
      121 cgacagaaac cgcttcggat ggcatcgggt ttgatgccgt ctgaagcggt ttcccgaaac
      181 gaaaacgtcc tttttcggcg gcaaactgta tcaacacgcg aaaatacaat aaaataccaa
      241 tatccgattt atttccaaca tcacggagag ccgtatgaaa tccagacacc tcgccctcgg
      301 cgttgccgcc ctgttcgccc ttgccgcgtg cgacagcaaa gtccaaacca gcgtccccgc
      361 cgacagcgcg cctgccgctt cggcagccgc cgccccggca ggactggtcg aagggcaaaa
      421 ctacaccgtc cttgccaacc cgattcccca acagcaggca ggcaaggttg aagtgcttga
      481 gtttttcggc tatttttgtc cgcactgcgc ccgcctcgaa cctgttttga gcaaacacgc
      541 caagtctttt aaagacgata tgtacctgcg taccgaacac gtcgtctggc agaaagaaat
      601 gctgccgctg gcacgcctcg ccgccgccgt cgatatggct gccgccgaaa gcaaagatgt
      661 ggcgaacagc catattttcg atgcgatggt caaccaaaaa atcaagctgc aagagccgga
      721 agtcctcaaa aaatggctgg gcgaacaaac cgcctttgac ggcaaaaaag tccttgccgc
      781 ctacgaatcc cccgaaagtc aggcgcgcgc cggcaaaatg caggagctga ccgaaacctt
      841 ccaaatcgac ggtacgccca cggttatcgt cggcggcaaa tataaagttg aatttgccga
      901 ctgggagtcc ggtatgaaca ccatcgacct tttggcggac aaagtacgtg aagaacaaaa
      961 agccgcgcag taggcccgtt tgaaaaatgc cgtctgaaac ttggttttca gacggcattt
     1021 tgattgggtt tagaacgtaa agcccgtttc cagttcttca tcgccgacca gttcgaccaa
     1081 gagcgcgtag agcggggcga gttcggcata acggcgcgat acgcggcgca gatagtttaa
     1141 gaaacgcggg atttccggac ggtatttgtc tttgccgtcg cggtagtaca ggcgtgcgaa
     1201 gatgcccgcg accttcaagt gccgctgcac gcccatccat tcgaaccggc ggtaaaactc
     1261 gtcaaactcg gcagggacag gcaagccggc agcccgcgcc ttttcccagt agcggataac
     1321 caaatccaaa acaaactctt cttcccactc gataaaggca tcgcgcaaca gcgacaccaa
     1381 atcgtaggaa atcgggccgt aaagcgcgtc ttggaagtct aaaacgcccg gcctgccgcg
     1441 cgtcagcatc aggttgcgga cgataaagtc gcggtgcacg tagactttgg gctgcgccaa
     1501 caagggcggc agcagcgtat cggcggtttg ctgccaaagt tggcgttgtt tgaatgttaa
     1561 ttcgcgcccc aattcttttg cgacaaacca ttccgggaac aggttgattt cgcgcagcat
     1621 cgtttcacgg tcatattcgg gcaaaacccc ttcacggctt gccttctgca atccgaccag
     1681 ctcgccgatg gcctccagca acagggcttt gtgtgccgct tcgccctgct cctgaagcat
     1741 cgcggtcaaa aacgtcgtat tgcccaagtc gttcaatacc acaaacccca aatccgtgtc
     1801 cgcgtgcaat acctgcggca cattgaccat gtcaaacagc ttctgcacct tcaaataagg
     1861 cgcgacactc atcttgtcgg gcggcgcatc catacagacg acgctgccgc cgtctgaaaa
     1921 cgccgcacgg aaatagcggc ggaaatcagc atccgccgcc gcgaaggaca gatcgaagtc
     1981 ccgttcggga taaacggtct gaagccaatt tttcagttcg gtttgccgtt gcataacagt
     2041 atccgggcat ttcgggttac aataaacact attctaaccg gtaaaccgac ttgaggggcg
     2101 attttggctc gtttattttc actcaaacca ctggtgctgg cattgggctt ctgtttcggc
     2161 acgcattgcg ccgccgatac cgttgcggcg gaagaggcgg acgggcgtgt cgcagaaggc
     2221 ggtgcgcagg gcgcgtccga atccgcacaa gcttccgatt tgaccctcgg ttcgacctgc
     2281 ctgttttgca gtaacgaaag cggcagcccc gagagaaccg aagccgccgt ccaaggcagc
     2341 ggcgaagcat ccgtccccga agactatacg cgcattgttg ccgacaggat ggaaggacag
     2401 tcgcaggtta aggtgcgcgc ggaaggaagc gttatcatcg aacgggacgg cgcagtcctc
     2461 aataccgatt gggcggatta cgaccagtcg ggcgacaccg ttaccgtagg cgaccggttc
     2521 gccctccaac aggacggtac gctgattcgg ggcgaaaccc tgacctacaa tctcgatcag
     2581 cagaccggcg aagcgcacaa cgtccgtatg gaaaccgaac aaggcggacg gcggctgcaa
     2641 agcgtcagcc gcaccgccga aatgttgggc gaagggcgtt acaaactgac ggaaacccaa
     2701 ttcaacacct gttccgccgg agatgccggc tggtatgtca aggccgcctc tgtcgaagcc
     2761 gatcggggaa aaggcatagg cgttgccaaa cacgccgcct tcgtgttcgg cggcgttccc
     2821 cttttctata cgccttgggc ggacttcccg cttgacggca accgcaaaag cggactgctc
     2881 gtcccgtccg tatctgccgg ttcggacggc gtttcccttt ccgtccccta ttatttcaac
     2941 cttgccccca acttcgatgc cactttcgcc cccggcatta tcggcgaacg cggcgcgacg
     3001 tttgacggac aaatccgtta cctgcgtccc gattacagcg gacagaccga cctgacctgg
     3061 ttgccgcacg ataagaaaag cggcaggaac aaccgctatc aggcaaaatg gcagcaccgg
     3121 cacgacattt ccgacacgct tcaggcgggt gtcgatttca accaagtctc cgacagcggc
     3181 tactaccgcg acttttacgg cggcgaagaa atcgccggca acgtcaacct caaccgccgc
     3241 gtatggctgg attatggcgg cagggcggcg ggaggcagcc tgaatgccgg cctttcggtt
     3301 cagaaatacc agacgctggc aaaccaaagc ggctacaaag acgaacctta cgccatcatg
     3361 ccccgccttt ctgccgattg gcataaaaac gcaggcaggg cgcaaatcgg cgtgtccgca
     3421 caatttaccc gcttcagcca cgacggccgc caagacggca gccgactggt cgtgtatccc
     3481 ggtatcaaat gggatttcag caacagctgg ggctacgtcc gccccaaact cgggctgcac
     3541 gccacttatt acagcctcga cagtttcggc ggcaaagcat cccgcagcgt cgggcgcgtt
     3601 ttgcccgttg tcaatatcga cggcggcaca accttcgaac gcaatacgcg cctgttcggc
     3661 ggcggagtcg tgcaaaccat cgagccgcgc ctgttctaca actatattcc tgccaaatct
     3721 caaaacgacc tgcccaattt cgattcgtcg gaaagcagct tcggctacgg gcagcttttc
     3781 cgcgaaaacc tctattacgg caacgaccgc atcaacgccg ccaacagcct ttccaccgcc
     3841 gtgcagagcc gtattttgga cggcgcgacg ggggaggagc gtttccgcgc cggtatcggt
     3901 cagaaattct atttcaagga tgatgcggtg atgcttgacg gcagcgtcgg caaaaatccg
     3961 cgcagccgtt ccgactgggt ggcattcgcc tccggcggca taggcgggcg tttcaccctc
     4021 gacagcagca tccactacaa ccaaaacgac aaacgcgccg aacattacgc cgtcggcgca
     4081 ggctaccgcc ccgcccccgg aaaagtgttg aacgcccgct acaaatacgg gcgcaacgaa
     4141 aaaatctacc tgcaggcgga cggttcctat ttttacgaca aactcagcca gctcgacctg
     4201 tccgcacaat ggccgctgac gcgcaacctg tctgccgtcg tccgctacaa ctacggtttt
     4261 gaagccaaaa aaccgataga aatgcttgcc ggtgcagaat acaaaagcag ttgcggctgc
     4321 tggggcgcgg gcgtgtacgc ccaacgctac gttaccggcg aaaacaccta caaaaacgcc
     4381 gtcttttttt cacttcagtt gaaagacctc agcagcgtcg gcagaaaccc cgcaggcagg
     4441 atggatgtcg ccgttcccgg ctacatcccc gcccactctc tttccgccgg acgcaacaaa
     4501 cggccctgac cgtcggaaac ccggcaggag caccgttcct gcacaagata gcattccacc
     4561 gacaacccca aacccgccat caaaggcagg attcaaacga taaggaaaga atgatgaaaa
     4621 tcaaagccct gatgattgcc gccgcattgc tggcagcagc cgatgtccac gccgcaccgc
     4681 aaaaggcaaa aaccgcgccc gccaaagcgg tcaaagctgc cgccacggcg caaaaagaag
     4741 ccgcacccgc acaacagcag ggcggtatcc gcttttcaga cggcattgcc gccgttgccg
     4801 acaacgaagt catcaccaac cgccggcttg ccgaagccgt tgccgaagcc aaagccaccc
     4861 tgcccgaaga cgcgcagata agtgaatccg agctgtcccg acaggtgctg atgcagcttg
     4921 tcaaccaatc cctgattgta caggcgggca aacgccgcaa cattcaggca agtgaagcgg
     4981 aaatcgatgc cgtcgtcgca caaaatcccg ccctcaaaaa cctcagcccc acccaacgcc
     5041 gcgagcttgc cgacaacatc attgccgaaa aagtccgcca gcaggcagtg atgcaaaaca
     5101 gccgcgtgag cgaagctgaa attgatgcct tcctcgagca ggcgcaaaaa caaggcatca
     5161 ccctgcccga aggcgcaccg ttgcgccaat accgcgccca acacatcctg attaaagccg
     5221 acagcgaaaa cgccgccgtc ggcgcggaaa gtaccatccg caaaatctac gaccaggcgc
     5281 gcaacggcac ggatttcgca ggtttggcac gccgatattc ccaagacgca agcgcgggca
     5341 atggcggcga cttgggctgg tttgccgacg gcgtaatggt tcccgccttt gaagaagccg
     5401 tccacgcgct caaacccgga caggtcggcg cgcctgtccg cacccaattc ggctggcaca
     5461 tcatcaaatt gaacgaagtg cgcgatgccg gcacgccgga ggaacgtatc cgcaattccg
     5521 tgcggcaata catcttccaa caaaaagccg gacaggcaac cgtcaacctg ttgcgcgacc
     5581 tgcattccgg cgcgtatgtc gacatccgct aaggcggttt gaatccaaaa gtcataccga
     5641 tcgggcaaaa gtccgggcgg tatggctttt ttgaatttcg agttactttt acaccgccat
     5701 tcgtcattcc cgcgcaggcg ggaatctagc aacgaaaagt aacaggaatt tatcgggaat
     5761 ggccggagtt taaaggtctg gattcccgcc ttcgcgggaa tgacgattca gaagttacac
     5821 gaaactcaaa aaaaaaaacg aaaccgaacg aaccggattc ccgcttttac gggaatgacg
     5881 gcatatcggt ttccgtgagg acaaacctag attcccgcct gcgcgggaat gacgggattt
     5941 tagattgcgg gtatttatcg ggaatggcga tttaaaaatt acctaaaact aaacttaaaa
     6001 gcaactgaaa cttaaaaaac taaaatttcg cctgcgtggg aaggatggga tttcagattt
     6061 ctgtttttgt gggaatttgc cgtatcgggc ttcagacggc attgcctgcc gttttgcccc
     6121 cgggtgcggc tgccttgatg tagttgagcg agacaaactg cttctcggca tccaattcgg
     6181 tgattttaaa caatgcctgc gatttgggca gcgcgtcaaa cggaataccg gtcgcgcgcg
     6241 ttgtcagtgg cagaccttcg atgcggacga ggtcttcttt cagaatcgtc gcggtcagtt
     6301 cgctgatgcc ttgttgttgc aggtacacga ggctccagta ggcttccatc tgccgttgga
     6361 agtcggcgta ggcggcgtag gcggtgtcga aatcgcgcag ggcggcgaag agcgcggcat
     6421 cgcttttttg atacagcggc tcggcagtgt cgtcgatcag gctgatcagc tgcttttggt
     6481 tgatgtagtc ggcggcgcgg cgcagcggcg aggtaaacca gccgtaatgc tgcacgccca
     6541 tgccgatgtg cggctcggat tgggtgctca tgcgcacttt gcctgccggt tggacgcgga
     6601 aaatgccggg cagcccgttt tcgtctagca tttgcgccca agtgctgttg gcaagaatca
     6661 tcatctcgct gaccagcgta tcgatgggcg aaccgcgttc gcggcggacg acggagacgt
     6721 tgccttcttc atccaattcg atgctgtaat cgtattgcgg cgcgcggtcg ggttcgtatt
     6781 tgccgcgcgc tttttgcagg gcggtggcga attgatagaa ccaaatcagg tcttgatggt
     6841 gggcgaacat catttcgcct gcttggtcca agccggtttc tgcgttgaaa tgcggctcga
     6901 tggcttggat acgcaggttt gcggcgatgt tgacggcttc gattttgcag gtcggctcgc
     6961 cgacgttgaa ctcgccgtcc acatcgaaat aaatgctgac ggcagggcgg tatgcgcctg
     7021 catcgaggct gaacgcggta atccagtttt cgggcagcat cgtgattttg ccgccgggga
     7081 aataaaccgt actcaaccgc tgcataatga tttgttccat tccgccgcct tgtcggacgg
     7141 caagcgacgg cgcggcgatg tggatgccga cgcgcttcgt gccgttgccc aaatcggtca
     7201 ggctcaaggc atcgtccact tcggtggtcg attcgtcgtc aatggaaaag gcggtaacgt
     7261 cggccttggg caggtcgggc atttcgggaa gggagaggtc ggggaaacct gttcctttgg
     7321 ggaagtattt gatttcaaag ccgtcttgca ggtattgcgg aatggacgta atgccgcccg
     7381 tttttttcgc cagttcgtag gcggaggttt tcagtgcgtc ggcggctttg gtaaaggctt
     7441 tgtaggtcag cgactgtttg tcgggcgcgt gtaagatggt tctcaaatcc gccgcgattt
     7501 cagacggcat ctcgccgcgt ttcaaggctt ccgcccaagc gtcgatttgc gcgtcttgct
     7561 gttttttgcg ttcgatggcg gcaagcgctt gtttcaaagt ttcttcgggc gcggctttga
     7621 acacgccttt ggcttttttg tagaaataca ccggcgcggc gtacagcgca atcaaagtcg
     7681 ccgccagctc ggttttggtc ggcgcatggc cgtaatattc ttcggcgatg gcttcggcgg
     7741 taaactcctc ttcgccgcat acttcccaca ataaatcggt gtcgatgtcc gccgcctgcg
     7801 cctgagcgtt ttccaaaaac gccgccatat cgccgtcaaa ctcggcaaag acattgtttg
     7861 ccttcacttt ggtgcgtttg ccgtgttggg tatcgacttg gtaggtagcg tcgttttttt
     7921 ggacgacgac ggcaactttg aactggccgg actcttcgta aaaaatattc atttttcgga
     7981 tttttctgtg gaaactcaag cgggcgattt tagcagatta ccgaaaatgc cgtctgaaaa
     8041 aaggttgggg gaaggcgggt gcggctttgc ggtgcttgcg ttgcgttttt gacggggtac
     8101 aatcgccgtt tttgaacgga ggacggaatg gagaatgtaa accgcgtgcc ggagcaggca
     8161 cgttatgatg ccgaacgcag gcaggcagac gaagcattgg cggacgtgtt tccggcagtc
     8221 agtattttcg gcagcgcgcg cacgccgcag gatcatgcgg attatgcgtt tgcctgccgt
     8281 ctggcgcggc ggctgtcgga ttcgggtatt gccgtcattt cgggcggcgg gccggggatt
     8341 atggaggcgg caaacaaggg cgcgtttgcc gggaagtcgg tttcggtagg gctgaacatt
     8401 gccctgccgc acgagcagaa accgaatccg tatcaggaca tcgccttgcg gttttcccgt
     8461 tttgccgaac gcaaggcagt gtttttccgc tattcccaag catatgtcgt gatgccgggc
     8521 ggcttcggga cgctggacga attgtttgaa atcctgacct tggtgcagac gggcaaagtg
     8581 ccgccgcgtc cggtcgtttt ggttggaaag gcgttttggt cgggcttggc ggagtggata
     8641 aacgcgcagc ttttggcgcg cggtatgatt tccgaagggg ccgcttcttt gttctccata
     8701 tcggacgacg aagacgaaat cattgcgtat ctgtcggaac acgggcttca gacggcatag
     8761 cgtcctgaga gtgatgtata attgcaaaca atttgtcaat ttttgatgtc ttttccgaac
     8821 aggatgccga tatgatcaac cccatcgcct cgctttcccc cctagacggc cgttatgccc
     8881 aatccgttga agcattgcgc ccgatttttt cagagtacgg actgatgaag gcgcgcgtca
     8941 aagtcgaatt aaactggctc aaagccctgg ccgccgcgcc ggagattgcc gaagtgccgc
     9001 ccttcagtgc cgaaacgctt gccgaaatcg acaaagtcat cgaaaacttt tcattagaag
     9061 acgcggccgc cgtcaaagcc atcgaagcca cgaccaatca tgatgtcaaa gccatcgaat
     9121 attggctgaa aaaacgtttt gccgaagtgc cggaagtcgc cgccgtgagc gagttcatcc
     9181 acttcgcctg caccagcgaa gacatcaaca acctgtccca cgccctgatg ctgcaagagg
     9241 cgcgcgaaac cgtcatcctg cccaaacttg ccgaaatcat cggcaaactc accgctatgg
     9301 cgcacgacct tgccgccgtc ccgatgatga gccgcaccca cggccagccc gccacaccga
     9361 ccactttggg caaagaaacc gccaatgtcg tgtaccgcct gcaacgccag ttcaaaatcc
     9421 ttcaggcgca agaattcctc ggcaaaatca acggcgcggt cggcaactac aacgcccaca
     9481 tggccgccta tcccgatgtg gattgggaaa cacactgccg caacttcgtc gaaatcagcc
     9541 tcggcctgac cttcaacccc tacaccatcc aaatcgagcc gcacgactat atggcggagt
     9601 ttttccaaac cctcagccgt gtcaacacca tcctgatcga ctttaaccgc gacgtttggg
     9661 gttatatttc attgggttat ttcaaacaaa aagtcaaagc gggcgaagtc ggttcttcca
     9721 ccatgccgca caaagtcaac cccatcgact ttgaaaactc cgagggcaac ctcggcatgg
     9781 caaacgccgt attgggcttt ttgtccgaaa aactgccgat ttcccgctgg cagcgcgacc
     9841 tgaccgacag caccgtattg cgcaatatgg gcgtgggcgt gggctatgcc gtgttgggtt
     9901 tcgccgccca cctgcgcggt ctgaacaagc tcgaacccaa ccctgccgcg cttgccgccg
     9961 atttggatgt cacttgggag ctgctcgccg agccgattca aaccgtaatg cgccgttacg
    10021 gtgtcgccaa tccttacgag aagctgaaag acctgacgca cggtaaagac ggcattacgc
    10081 ccgaagtgtt gaaactcttt atcgaatcgt tggaaattcc ggcagaagcc aaagccaaat
    10141 tgcttgagct gacccctgcg ttgtatgtgg gcaaggctga agcgttggcg aaacggattt
    10201 gagtgcttat tgaaaccgat gccgtccgaa cgcgcgttca gacggcattt ttaagataac
    10261 gggacatacg gggcgatatt tatgcaagct gtccgataca ggcctgaaat tgacggattg
    10321 cgggccgtcg ccgtgctatc cgtcattatt ttccacctga ataaccgctg gctgcccgga
    10381 ggattcctgg gggtggacat tttctttgtc atctcgggat tcctcattac caacatcatt
    10441 ctttctgaaa tacagaacgg ttctttttct ttccgggatt tttatacccg caggattaag
    10501 cggatttatc ctgcttttat tgcggccgtg tccctggctt cggtgattgc ttctcaaatc
    10561 ttcctttacg aagatttcaa ccaaatgagg aaaaccatag agctttctac ggtttttttg
    10621 tccaatattt atttggggtt ccgattgggg tatttcgatt tgagtgccga cgagaacccc
    10681 gtactgcata tctggtcttt ggcggtagag gaacagtatt acctcctgta tccccttttg
    10741 ctgatattct gttacaaaaa aaccaaatca ctacgggtgc tgcgtaatat cagcatcatc
    10801 ctgtttctga ttttgaccgc atcatcgttt ttgccggccg ggttttatac cgacatcctc
    10861 aaccaaccca atacttatta cctttcgaca ctgaggtttc ccgagctgtt ggtgggttcg
    10921 ctgttggcgg tttacgggca aacgcaaaac ggcagacggc aaacagaaaa tggaaaacgg
    10981 cagttgcttt cattactctg tttcggcgca ttgcttgtct gcctgttcgt gatcgacaaa
    11041 cacgatccgt ttatcccggg aataaccctg ctccttccct gcctgctgac ggcgctgctt
    11101 atccggagta tgcaatacgg gacacttccg acccgcatcc tgtcggcaag ccccatcgta
    11161 tttgtcggca aaatctctta ttccctatac ctgtaccatt ggatttttat tgccttcgcc
    11221 cattacatta caggcgacaa acagctcgga ctgcctgccg tatcggcggt tgccgcgttg
    11281 acggccggat tttccctgtt gagctattat ttgattgaac agccgcttag aaaacggaag
    11341 atgaccttca aaaaggcatt tttctgcctt tatctcgccc cgtccctgat acttgtcggt
    11401 tacaacctgt attcaagagg gatattgaaa caggaacacc tccgcccgct gcccggcgcg
    11461 ccccttgctg cggaaaataa ttttccggaa accgtcttga tcctcggcga ttcgcacgcc
    11521 ggacacctgc ggggttttct ggattatgtc ggcagccggg aagggtggaa agccaaaatc
    11581 ctatccctcg atccggagtg tttggtttgg gtggatgcga aactggcaga caatccgtta
    11641 tgccggaaat accgggatga agttgaaaag gccgaagctg ttttcattgc ccaattctat
    11701 gatttgagga tgggcggcca gcccgtgccg agatttgaag cgcaatcctt cctgataccc
    11761 gggttcaaag cccgattcag ggaaaccgtc aagaggatag ccgccgtcaa acctgtatat
    11821 gtttttgcaa acaatacatc aatcagccgt tctcccttga gggaggaaaa attgaaaaga
    11881 tttgctataa accaatacct ccggcctatt cgggctatgg gcgacatcgg caagagcaat
    11941 caggcggtct ttgatttggt taaagatatt cccaatgtgc attgggtgga cgcacaaaaa
    12001 tacctgccca aaaacacggt cgaaatacac ggccgctatc tttacggcga ccaagaccac
    12061 ctgacctatt tcggttctta ttatatgggg cgggaatttc acaaacacga acgcctgctc
    12121 aagcattccc gaggcggcgc attgcagtag cctgccttct tgtcggatat tgcctttggc
    12181 agcctatgcc gctgtttgcc ctttgaggcg gcttttattt tgccgttttg aggccggggt
    12241 cgggaataac cgttttttga tgattttccc tccccggctg tgtcatcaaa tccctagttg
    12301 cctttccaaa ctctccacca gattgtcatc cagtttcaaa gcctgcgaca ggcgggcgag
    12361 gaagacggtt tctttccgcg acaaatcggc acagaccaac cttgccgcca gataggcctc
    12421 cgccgccaac gcctcatcgt tgccgacggc ggcggcgatg tccccgatgc ttgcgggcag
    12481 gcggtattcg gcggcgagcc atgcggcagt ttcggggtct gtgccgcttt cctgttcgat
    12541 agtccggcgt tcggcttcgt ctatcatgcc gtctgaagcg gcggcggcta tcatggtacg
    12601 caatacggtg cggctgtatg tttcttcggt ttttccggca ggttggaaat cgctttgtgt
    12661 tacggttgcc cgccctttgt tttgctgcca catctgatag ccccggtagg cgaggtagcc
    12721 caaagcggcg gtcgaaccga ttttggtgat ggttttgcgg tttttaccgt tcagcagcat
    12781 ggaggcgaca ccggcaacca gcgcgcctcc gccgaatgaa ttgagcgggc tgccggagaa
    12841 tgtgttgcct tttttttgaa ccgtgcttaa gacttggttg agcaaccggg tgaagttcat
    12901 gatttttcct ttctgttgcg ggaagggcgg tatgtttacc ctatcctttt aaacggtggc
    12961 aggccggcca ataattgttg gccgtaccgc tgcgttttga tgcggttgtc gaggatggtt
    13021 acgcggccgt agtcttgttc ggtgcggatg aggcggccga cggcctggat gagtttgatg
    13081 ccggcttcgg ggacggtgat ttcgatgaag gggttgccgc cgcgttgttc tatccaacgg
    13141 ttttgggttt tttcgatggg gttgtcgggc atggcgaagg ggagtttggc gatgatgact
    13201 tgcacgcagg cggtgccggg caggtcgagc ccttcggcga agctgtcgag tccgaagatg
    13261 atgctggctt tgccttcttc tatggcttgg tggtgttttt gcaggaggac ggctttgggt
    13321 aattcgcctt gtacgagcaa gagcggcagg tagtctccgg gcaggtgcag ggcgacatcc
    13381 tgcatttgtt tgcgcgagga aaacaggacg agtgtgccga tggcttcggt gggcgaaatc
    13441 agcttgggca gccattcgat gacggcggcg gtgtgggctt cggggtcttt ggggctggcg
    13501 tatatggagg ggatgtagag ttcgccctgt ttttcaaagt caaaggggct tttgagggcg
    13561 agggtggtgg tttcgggcag ccacagcagg ccggtttggc gcaaaatcaa gttgaagctg
    13621 ccgagcgatt gcagggtggc cgaagtcagc accgcgcctg ccgcgcgccg ccacaggctg
    13681 ttggcaaggt gggacgcgct gctgatgggg ctggcattga aaatatagtc gtttttgtcg
    13741 tcggcacggc gggctatcca tttcgccaac ggctcctcgc cctcaatgga aacagtggag
    13801 agcaaatccc aaaccgcgct gatttgttcg atacgggcga taaaaaggcc gaactcgccg
    13861 gtcaggcggt cgatgagcgc accgtcctgt tctttttcgc ggcgtgcggc ggaaagcgca
    13921 tcgttcagcc cgacaacgtg tttgagcagg ctgcgtgcag caatggccgt attggaaacg
    13981 gtggtttcga ggccttcggg gattttgccg tcttcccaca gccaagtcgg ttcgctgttg
    14041 gttcgtctgt cgttttcaga cagccccata cttaaagacg gctcttccgc caaatggaat
    14101 tgccattcgt gcaggctgtc gagcaaggat gcggcggctt cgtcggcaag gttggcaagt
    14161 tcggctttat cggtaagcgc ggcaattttg ccggtaagct gcggcagttt ttccagcgtc
    14221 caaacggcaa tattccatga atgttcggcg gcaaaacggc tgagggcttt tttgggcagg
    14281 tggtgcgctt cgtctatgca atagaaactg ttttcgggcg caggcagaat cacgccgccg
    14341 cccatgctga tgtcggcaag cagaagatcg tggttggcaa caacgacatc gacggtttcc
    14401 aacatatcgc gtgccaggta aaaagggcat tccggacggt tgggacaggc ggctttcagg
    14461 cagccgtggc ggtcgttggt cactttgagc caaatcgcgt catcgatttt ttccggccaa
    14521 gcgtcgcggt cgccgttgaa ccgtcgggcg gaaaattcgt cggcgatgtc gcgcagcagc
    14581 ttcaattctt ccggcttggg tttgctgtcc cacaagacgg cgggggcttc aaagccgagc
    14641 aggttttgct gggcgttgct ttgcgtcagt tgatagagtt tgtaggggca gagatagcgg
    14701 ccgcgccctt tggcaagtgc gaaggtcagt tccaaaccgc ttttttcgac cagaaacggc
    14761 aggtcgcggt ctaccaactg ctcctgcaag gcaaccgtcg cgctgctcac aatcagccgc
    14821 ttgccgcgtg tttgcgccat gatgccgccc gcaagcaggt aggcaagcga tttgcccacg
    14881 ccggtcggcc cttcgattac ggcaatgctt tcgccctcgc gcttgggcgg ctcgccgcct
    14941 tcttcgcgcg tcaacgtccg cgaaaaagcg ttggcaaccg ccgcaatcat ttcccgctgc
    15001 gaagcacgcg gacggaaacc gggcaggttt ttgccgatat tttggtaatg gtcgcggatg
    15061 gcgttttttt ctaaatcggt gagcatagta tcggtgggcg gatgatttgg ggtcatttta
    15121 acattgcgcg gatgcggtac ggtatcgttg tcggacggtg gttcgggcat atctgattat
    15181 tgaaagtaaa aaaacggaag atattgggtc atccgtacaa aatggcgtct gaagcgggaa
    15241 acgggcttca gacggcattg ccgcgcttat acgccggcga cggggcgggc gggatagccg
    15301 tgggtatcga ccgaacaggc aaaccgccaa ggcgtgtgga cggtgtcggc ggacaggtgg
    15361 gcgagttcgg gaatgtgccg tttgataaaa gtaccgtccg ggtcggtacg gtatgaggcg
    15421 gcggcaatgt cggggcaggt gtgccgtgag gcggcaagcc gccagttgcc ttggttgatt
    15481 gccgcatcga aatcggtcag ctgccgggca aaccatatct cgccttcgcg gcgggggagg
    15541 tttaaaacgt ggcagaaaaa atccgcgctc aagcgtctca gggcggggtg gaggctgccg
    15601 gttttgtgca aacagcgcat cgcggcatcg ataatcggaa tgccggtccg gccctgctgc
    15661 caaagcgtca ggcgcagggt gtgttccgga ttgccgtctg aagggtcgtc atccgcgtgc
    15721 tgcaaggcaa gttgaaggaa aaaatcgcgg cggatgatgt tgtccgccca cgcgttcaga
    15781 cggcgttcga ggctttcccg cgcgagcagg cgcggcgaga tgcagccgac actcaaatac
    15841 gcgcccatca gcgaagtgtt tttgcgcgaa gggaaatcct ttaaaacgga gtaggaagcc
    15901 gcctgttcga gaaaccgccg ccactgccgc caagccgccg tttcgccgcc gttttgcggc
    15961 aggaagatgc cgtctgaaag cgcggcaggc ggcggggcgg gcaggttttc ggggaagggt
    16021 tggcggtatg ccgcgaatag gtccgaaccg gcggggggct gcttggaaaa gcggtcgagc
    16081 catacttcgc ggtagcggtt gaaatcggtg tgtgccgtac cgttgtctgg tgtcaggtcg
    16141 gttttgccga aaacggaacg gtcgttgacg aaggttaacg cgatgccgtg tttgtccaat
    16201 tcgtgccaaa gggcgttgtc ggcgagtttg tcggcaaaag tatgggattc gtcggcgatg
    16261 acggcgcgga tattgaggcg gacggcgagc cggacaagcc cggcaggcga tgccgccgtg
    16321 tagagcggga tgccgcgcct tgccagccct tgggcgagtt cggcggcgga ttggcggtag
    16381 aacgcggcgc ggcgcgggtt gtctgtttcg gcatcgtcaa tccaaatgcc gataacgggc
    16441 gaaccttcgg caacggcggc gcataaggcg gcgttgtcgc ggatgcggag gttttggcgg
    16501 aaccagacga gcgtgtgcgc ggcgcgcgtg tccgcataaa ggaggagggc ggtttcagac
    16561 ggcatttcgg cagcctttcc tgctggcgat tttttcgttc agaaaatcga tgaaactgcg
    16621 gactttcgca ctcaaaaatg ccctgtccgc ataaacggcg ttcagccggt cggtcgggac
    16681 ggcgtatccg ggcagcagcc tcaccagcgg gccgcagcgc aaatcgtgtt ccgccgccca
    16741 aagcggctga taaccgatgc acgcgcccgc cttaatcatt tcgcgcatca tcagcgtgtt
    16801 gtcggtacgg atgacggggg tcagttcaag ccggtatttc ttgccgtccg atttgcgggt
    16861 gaggtcgagt ttctgctggt tggtgtaggt cggcaggacg gcaggcagcc ccgccacttc
    16921 ttccggtgtt tccggcacgc cgttgcgcct caggaagtcg ggcgaggcaa gcagggcaaa
    16981 ttcgatttcc gccagcgggc gcgcaatcag cgaaggggaa agggtttggg aaacgcgcaa
    17041 cgccaaatcc acgccttcgg caatcaaatc gacgtggcgg ttgtccaaaa tcagttccaa
    17101 cgccacttcg ggataacgtt cgcggtattc cgccagccag ttgcatatct ggctgccggc
    17161 aaaccacagc ggcatcgtta cgcgcagcag cccctgcggt ttttccgtcc ccccggcggc
    17221 tttttgcgcg gcatcgtcga gcgtgtcgag cgcgtaactg cattgccggt agtattcttc
    17281 cccggcttcg gtcaggctga ggttgcggct gttgtggtgc aggagtttgg cttggacggt
    17341 gttttccaag tgactgacgt gtttgcttgc cattgcggtg gagatgccga gcgcgtcggc
    17401 ggcgcgggtg aagccgccgc tttggacgac ttggcggaaa actttgaggc tgaacagggt
    17461 gtccatattt tcttgtgtgg aaaagttgta tcaataaaag cagtatatat ttgaaaaagg
    17521 gaaacatcta tactctgccg cctgaaatga agacaaatat caaaggagct tttatgtccg
    17581 attgctgcaa ccgtatccaa ccggttttgc tttctgtttt gcgtatcgta accgcctacc
    17641 tgtttttgtt gcacggtacg tcgaaaatct tcgccttccc cattgaaatg ggcagcggtt
    17701 cgcccggcgg gctgttgctg cttgccggta ttttagaaat tgtcggcggc attttgctgg
    17761 tgttgggcct gtttgcgcgc cctgccgcgt ttgttttgtc cggccagatg gcggttgcct
    17821 attttatggc gcacgcttcc ggaaatgctt tgttcccgat tgccaacggc ggcgagtccg
    17881 cagtgctgtt ctgcttcgta tttctctata tcgcggcagc gggcggcgga gcatggtcgc
    17941 tggacaggct gtttttcaag cgtaaagcct gaaccggact gcctaaagtg tattttgttg
    18001 aatgtttttg aggaaaagaa atgagccgtc aatccctgca acaggctgcc gaaagccgcc
    18061 gttccgttta ttcgttaaat aaaaacctgc ccgttggcaa agatgaaatc gtccaaatcg
    18121 tcgaacacgc cgttttgcac acaccttttt cgttcaattc ccaatctgcc cgcgtggtcg
    18181 tgctgtttgg cgaagagcat ga
//
LOCUS       DAXDXW010000046        18128 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXDXW010000046 DAXDXW010000000
VERSION     DAXDXW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18128
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.047"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            183..2189
                     /locus_tag="ACFB64_001301"
     CDS             183..2189
                     /locus_tag="ACFB64_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC1209655.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFNPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVGRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGGIIALMALLKIRIGTLDLSPFLASLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLSKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIINHPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGFGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKGQGHPPSDKP"
     gene            complement(2312..3403)
                     /gene="mltB"
                     /locus_tag="ACFB64_001302"
     CDS             complement(2312..3403)
                     /gene="mltB"
                     /locus_tag="ACFB64_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFC1209656.1"
                     /translation="MEKRKILPLAICLAALSACTAMEARTPRANEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSQAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGRAKFHGARRFYAENRAVIDDVAQKYGVPAELIVAIIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGETLADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(3551..4978)
                     /locus_tag="ACFB64_001303"
     CDS             complement(3551..4978)
                     /locus_tag="ACFB64_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC1209657.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEGK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGGEAQVAQGLDMIFKGEGGGLGVTEPETSGAAIAREMQSCYEYTDEQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSRGDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILDNLNRYVRNDIRFV
                     DYERREIQRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHPAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGIKLDKN
                     QTLLDRLQTDVRFAAVARDYALPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     EEHEDTTAFLLNDIRKDILDCRYCG"
     gene            5163..8867
                     /gene="mfd"
                     /locus_tag="ACFB64_001304"
     CDS             5163..8867
                     /gene="mfd"
                     /locus_tag="ACFB64_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC1209658.1"
                     /translation="MTCPIPKPREKSRWFNLSQGSLPLALARYLPHKRLKAVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSETPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWNDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKAHSLPDLAVNRQSDEPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSSSQQQTVPASEGEGKAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDGLKAAAVSTESSLYLVASDLHGQTRQQSAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRDLAEINIG
                     DPVVHEEHGIGRYTGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     SVALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGHKFEINESDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKATLEGMADGTVDIVIGTHK
                     LVQDDIKFKNLGLLIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMMQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPEDYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIE
                     SHHLRLAAKELGIDAIDATSEAVTVTFGKHHCIDPTGIILLIQTDKKYRLAGADKLRF
                     AAEMENIEVRINTVKTVLKTLQGKRLPKGN"
     gene            9093..9395
                     /locus_tag="ACFB64_001305"
     CDS             9093..9395
                     /locus_tag="ACFB64_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2004 domain-containing protein"
                     /protein_id="HFC1209659.1"
                     /translation="MDAFAAFLSELEKADDAARAALTEYLKRDSRYIDFHTEDTQTSR
                     DAAKFVRTMQLIYISLWAKNPAFAVMDYMPANMESDEILAVKLHLDGSIFSIDWES"
     gene            9557..9940
                     /gene="panD"
                     /locus_tag="ACFB64_001306"
     CDS             9557..9940
                     /gene="panD"
                     /locus_tag="ACFB64_001306"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC1209660.1"
                     /translation="MFRTILGGKIHRATVTEADLNYVGSITVDQDLLDAAGICPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYIQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            9962..10804
                     /gene="kdsA"
                     /locus_tag="ACFB64_001307"
     CDS             9962..10804
                     /gene="kdsA"
                     /locus_tag="ACFB64_001307"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC1209661.1"
                     /translation="MDIKINDITLGNNSPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLENFLIRIKALDDLIKSQPI
                     LTIE"
     gene            10819..11259
                     /locus_tag="ACFB64_001308"
     CDS             10819..11259
                     /locus_tag="ACFB64_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC1209662.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGTVLAAAFAALTRRQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSVAGGSFGMWHGISSMTFMATALLSAVLSWRLSGKEAV"
     gene            11306..12592
                     /gene="eno"
                     /locus_tag="ACFB64_001309"
     CDS             11306..12592
                     /gene="eno"
                     /locus_tag="ACFB64_001309"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC1209663.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEAAEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGKKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKCNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLGK"
     gene            12760..13038
                     /gene="ftsB"
                     /locus_tag="ACFB64_001310"
     CDS             12760..13038
                     /gene="ftsB"
                     /locus_tag="ACFB64_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC1209664.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(13079..13369)
                     /gene="yfaE"
                     /locus_tag="ACFB64_001311"
     CDS             complement(13079..13369)
                     /gene="yfaE"
                     /locus_tag="ACFB64_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC1209665.1"
                     /translation="MARIGTNKGLFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLAGEDEGLSDSLLK"
     gene            complement(13490..14623)
                     /gene="nrdB"
                     /locus_tag="ACFB64_001312"
     CDS             complement(13490..14623)
                     /gene="nrdB"
                     /locus_tag="ACFB64_001312"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC1209666.1"
                     /translation="MSYSTFPKTKNDALKEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWVETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDPEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLSAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(14936..17215)
                     /gene="nrdA"
                     /locus_tag="ACFB64_001313"
     CDS             complement(14936..17215)
                     /gene="nrdA"
                     /locus_tag="ACFB64_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC1209667.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYNHVKKITDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEARLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVSGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPDGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMDRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGNKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            17746..18057
                     /locus_tag="ACFB64_001314"
     CDS             17746..18057
                     /locus_tag="ACFB64_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFC1209668.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHISKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRTLPYAEQKIQCRAAYEAFDDFDGGRFR
                     R"
BASE COUNT         4393 a         5110 c         4481 g         4144 t
ORIGIN      
        1 ccgaaattgt tataatcgaa aaaattaaaa atcaatacgg attattctat cagattgatt
       61 tatcgatatt tattatatca ttaatatagt ttgatttcaa accggcggaa aatgcgccgc
      121 ccgccgcaga tgcgccctcc ccgccatcgg ggcgtttaac gccggattgc cgatgcggta
      181 caatggcaga tatgaagaaa attacccctc aaaacctgcg tcccctgctt tcggaaagct
      241 tgggacatac cgattttgtc aacgtcctca acgcattgat taaatttttg cgccgtggcg
      301 gcaaaaaatg tgcgggggag cgtttcgacc tgattatcga cacattcaaa caagacaggg
      361 aattactgtc ccgcttcagc cggtgttttt acatctggct cgcgcaaata cacatttatc
      421 cggcactcat caaactcggc attttctcgc gccacagctt tgcccgggaa atgggcatac
      481 gcatatacga acgcttcaac ccgtcatata aagattttgc caacttgggc gaagtcttcc
      541 tttatctttt ccattccgaa aacgacgaca aatggctgca aacgctcaat atccgccaat
      601 ggctggtttt atacgaactc atccgtagcc acgccgagcc gtccaaattg cagacggcgg
      661 gcatccgcct tgccgatgcg cgtttgcgcg ccatcgaaat gctgtctgtc tggacggcat
      721 ccgaagccat cgaacccgat ctcatccgta tcgccccgcg cctgctggaa gccgattctt
      781 ccttcgtcgc cctccaacgc gaaaccgcca aactggtcga acactaccgc aacggcaccg
      841 cgccttacga caccgcccac ctcgaagtga tgttcgacca atgtttcagc cagattgact
      901 atttgcgtcg caaagggacg ggcgccggct ccggttcgtc ggtcaaagtc gcccacctgc
      961 tcgaacgact ccggcagacc gtaggccgtc tgaagctgct cacagacata caaaccggtg
     1021 ccggcaacag caaccgcctg accatcgccc tgatgaactc cctcatctac gcggcggtcg
     1081 aacaatacag cacccgccac ctgcgccgca gcagcatccg tatgctcgcc cgcagcatta
     1141 ccgaaaacaa aagccaccac ggcgaacact acatcacccg caaccgcaaa gaatatttca
     1201 aaatgttcta ctcggcagca ggcggcggcg gcatcatcgc cctgatggcg ctgctcaaaa
     1261 tccgcatcgg cacactcgac ctcagtccct tcctcgcttc cttgtcggct gggttcaact
     1321 acggcatcgg ctttatgatc atccatatgc tgcactgcac cgtcgccacc aagcagcccg
     1381 cgatgactgc cgccagcttt gccgaacagg tcgatctcaa cgaaggcggc aaagcggtgg
     1441 acaacaaact ctccaagctc ctcatcgacg tatgccgctc ccaaagcgtc gccgtcttcg
     1501 gcaacgtttc catcgccatc cttttggcgt gcgccatatc gttcggctat gcccatctgt
     1561 accggctgcc catactcgat gcccacaccg ccgcctacca gttcaaatcc atagacatca
     1621 tcaatcatcc taccctatgg tatgccgcta ttgcaggctt gtggctgttc tgctccggca
     1681 tcatcgcagg ttttttcgac aaccgcgccg actacctcaa cctgcgccaa cgcctgccct
     1741 tcaatccctt gctgcgtaaa atcatgcgcc ccgggccccg ccgcgtcctc gccgcctaca
     1801 tccacaaaca ctacggctcg ctggtcggca acttcatctt cggaatgctc ttgggtatga
     1861 ccggctattt cggacacctc ctcgggctgc cgctggacat ccgccacgtc gccttttcct
     1921 ccgccaacct cggctatgcc gccgtcagcg gcaacgtcgg ctttggtaca ttcgtactcg
     1981 gcattttcag cgtcctcgcc atcggcttgg tcaacctctg cgtcagcttc agcctcgccc
     2041 tcttcgtcgc cctgcgctcg cgcggcacaa aaatcggcag catccgcaac ctgatcaaaa
     2101 gtttttggaa tcagattaaa agcaatcctt gcatactttt cctcccgccc gccaaaggac
     2161 agggacatcc tccttcggac aagccttgac cggcaatgcc gtctgaaacg ggattcgccc
     2221 cgaataccgc cctgatgcgg gaaatcccca taaaaggatg caaaaatgcc gtctgaaccg
     2281 aaacgtggtt cagacagcat tttaaaaaac attacaatcc cgggccgccg agcgaattgg
     2341 caatgtccct gaccgccgtt acatacatcc ggctgtggtt gtactgccat accgtataaa
     2401 aattgttcaa gcccaaataa tattcaaaca cgccgggtgc ggtttccagt ttgaacaaaa
     2461 ccgccttttc atcatctgcg agcgtttccc cggggatgat gccgtacgcc ttcaaatccg
     2521 ccaccgtccg cgtcagggcg gttttttcgc caatgattgc ctgaacatcc gcacccggcg
     2581 ccaacgtcgc cgacaccaac attttaccgc ccgtgcgcca accgtgctgc ttcatataat
     2641 tggcaaccga tgccgcgaca tcaccgacgt tgccccatat atcccgatgt ccgtccccgt
     2701 cataatccac cgcccatttc cggtagctcg aaggcataaa ttgcggcata cccattgcgc
     2761 ccgcatagct gcccttaaag gcgaaaacat cgccgccttc ttcttttgcc agctttaaaa
     2821 gctcgaccaa ttctttttgg aaaaacccgg cgcggcgggg ataatcaaag cctaaggtcg
     2881 ccaatgcgtc cgccacacgg aagctgcccg tatttttgcc gtaattcgtt tcaatcccga
     2941 taatcgccac gataagctcg gcaggcacgc cgtatttttg cgccacatca tcgataaccg
     3001 cgcggttttc cgcataaaac ctgcgcgcgc cgtgaaattt cgccctgccc gaatttcccg
     3061 tgcggaacac ataccacgga cgcgatgtgg agggtcggtg cataatcttg acgatgtccg
     3121 ccttgtaagc cgctttgtca aaaaaatcct gccattccgc ctgggaaaaa tcccctttcc
     3181 cgacttcatc gtccacaaaa cggcggacat ttgcattggc ggcaaacccg ctgtcggata
     3241 ccggtacggc tgccgcgtca aacgcggggc ggctttcttt tttcatttca tccgcgcggg
     3301 gggcttgggc ttcatttgcc cggggtgtgc gggcctccat cgccgtacag gcagacaaag
     3361 ccgccaaaca aattgccagt ggcagtattt ttctcttttc cataaacaca ttccgaacaa
     3421 atagggtaag tgggaaagcg gcacaagggt gaggcgcgaa atgcggtatc cgccgcaatc
     3481 ggcggttttg cagaatgccg cgcgttgcct tttgcgccgc ccgaaatctg cctgccgccg
     3541 tatttgcccg ctatccgcaa tatcggcagt ccaatatatc tttgcggatg tcgttcagca
     3601 ggaaggctgt ggtgtcttcg tgttcctcct gtacgaagac aaagagtcgg gcgatgattt
     3661 tgccgatgaa gactttttgc agggcgcagg cgtcggtcag ggtgtgttcc tgcaggaagc
     3721 tgcggatgtc ttcgggcaaa gcgtagtcgc gggcaacggc ggcaaagcgg acatcggttt
     3781 gcaggcggtc gagcagggtt tggtttttgt ccaacttaat gcctgcttct ttttcttcgg
     3841 cggtggcgcg gacgaactgg tcgtaaattt cggcataaag catatcgttc gggccttcaa
     3901 aaatcgtgaa ggggcggata tcgatggcga tattgccggc tgggtgtccg cgttcaaaac
     3961 ccttcgcgcc caagagtttt tgcaacattt gcgcggcggc gtaagtgtat tccgtggcga
     4021 gggttttgac gatgttcgcc tccatcaatt gatgggcgac gggcgcgacg ggcgaaacgg
     4081 aatggcagac gtagcggtaa aggatttcgg aaacctgatg gcggcgttgg atttcgcggc
     4141 gttcgtaatc gacgaatctg atatcgttgc ggacatatcg gttcaggttg tcaaggatgt
     4201 attccataat gccgtgcgtc atgccgatca gttgcaggcg gctgcggata aagatgtttt
     4261 ggaacgcgcg caaaccggca gcgtcgcccc gggagagttt catcacggcg gttgcaggca
     4321 tttcggcatc gatgcggttg acggcgtaac ggacggcgcg caggccttcg gatgcgaggg
     4381 tttcgcagcg gatgtatgtt ttggggacga gcagcaggtc gatgactttg gcgagtttgc
     4441 cgtttttgcg ctctttggcg gcaacgagga ggaagtcgct ttgcgaattg ccctgccagt
     4501 atttcgcggc gttgacgtaa atggtttgtt cgtcggtata ttcgtagcag gactgcattt
     4561 cgcgtgcaat cgccgcgccg gaggtttcgg gttcggtaac gcccaaaccg ccgccttcgc
     4621 ctttgaaaat catgtccaaa ccttgtgcga cttgcgcttc gccgccgaac tcttgcagag
     4681 gctgcaacac cagcgcgcct tcgatgccgg tacgcagcgt aacgggcacg ccgtaatgcc
     4741 ccgcaatcct taggacttct tggatttcaa actgactgcc cttgcgcccg ccgtgttttt
     4801 tgtcgaggaa gggcaacagc aaacccgcct gcttcaaggc aagccatttg ccttcgggca
     4861 ggtatcgcat caggtcgatg ccgtctgaaa aaatgcggcg gaatgcggat tcgatgtgct
     4921 ttaaaaaagc agccgtgtcc atggttgacg gctgcgcgct cggttcggtg tgtatcatcg
     4981 gcttcctctg tcggttccca ttaatcggcg gccggtcaaa ccgcctgcca cagtttagag
     5041 ttaattttct aaactttacc acaaagtgca ccgggcaaca atccgccggc ctttcagacg
     5101 gcatcgcgca ccctcacgtg ctaaaatgac cgtttgcatc actgtccgcc gattgccgca
     5161 ctatgacctg ccccatcccc aaaccccgtg aaaaatcccg ttggttcaat ctttcgcaag
     5221 gctcgctgcc cttggctttg gcgcgttatt tgccgcacaa gcggctcaaa gccgtgctga
     5281 cccaagatgc ggaacaggcg ttgcgccttc agacggcatg gcggtttttc cgtccgcacg
     5341 acacggcggt gttcctgccg gactgggaaa cgctgcctta cgagcgtttt tctccgcatc
     5401 aggatttggt gtcggagcgg ctgtcggcgt tgtggcagat taaaagcggc gcggcggacg
     5461 tgttgttcgt accggttgcc acggcgatgc agaagctgcc gcccgtgccg tttttggcgg
     5521 ggcgcacgtt ttggctgaaa acggggcaga ctttggatat aggccgcctg aaaaccgatt
     5581 tggtggatgc gggctacaat catgtttccc acgttgtcgc ggcgggcgag tttgccgtgc
     5641 gcggcggcat tgtcgatttg ttcccgatgg gcagcgaaac gccgtaccgc atcgatttgt
     5701 ttgacgatga aatcgacagc attaaaacct tcgataccga cacgcagcgc accatttccc
     5761 ccgtttccga aatccgcctg ctgccggcgc acgagttccc caccgacagc gaggcgcaaa
     5821 aaatcttccg cagccgcttc cgcgaggaag tcgacggtaa tccgaacgat gccgccgtgt
     5881 acaaagccgt cagcaacggc cacttcggcg cgggcgtgga atattatctg ccgctgtttt
     5941 ttgaaaacga gctggaaacg ctgtttgact atatcggcga agatgcgctg tttgtctctt
     6001 tgggcgatgt tcatgccgag gcaaaccgct tttggaacga cgttaaatca cgttacgcca
     6061 tggcgcaggg cgacgaaacc tatccgcctt tgcttccaca gcatttgtat ctctctgccg
     6121 atgtgttcgc aggccgtctg aaaaactacg gacaagttct gcctgatgtt tccggcaagg
     6181 cacactccct gcccgacctt gccgtcaacc gccaatccga cgagcctttg caggcattga
     6241 aggattttca gacggccttt gacgggcgga ttttgctgtg tgccgaaagt ttgggacggc
     6301 gcgaaaccat gctcggtttc ttgcagcaaa acggtttgaa agccaaacct gtgtccgact
     6361 ggcagggctt cttgtcggcg cacgagccgc tgatgattac ggtcgcgccg ttggcttacg
     6421 gattcaaact gggcggactg caatcttcaa gccaacagca aactgttccc gcctccgagg
     6481 gagaaggcaa agcggttacc gaccaaaccg aattttccgc atccgcaaca aaccctctcc
     6541 ccagccctct cccgcaggag agggaacaaa gtgccgccgc cgtttcagac ggcctgaaag
     6601 cagccgccgt ttcaaccgaa agcagcctgt atctcgtcgc aagtgatctg cacgggcaaa
     6661 cccgacagca atctgccccc tcccccgtgg gggagggttg gggagagggc aaagcggttg
     6721 ccgctcaaag tgccatcgcc gtcatcaccg aatccgatct ttaccaatac gtcgcccgtt
     6781 cccgcgtcca caaccgccgt aagaaacacg ccgctgtttc agacggcctg ttgcgcgacc
     6841 ttgccgaaat caatatcggc gatcccgtcg tgcacgaaga acacggcatc gggcggtata
     6901 cgggcttggt aacgatggat ttaggcggcg aaaccaacga aatgatgttg ctcgaatacg
     6961 cgggcgaagc gcagctttat gtgcctgttt cgcaactgca tttaatcagc cgctactccg
     7021 gtcaggcgca tgaaagcgtc gccctgcaca agctcggcag cggcgcgtgg aacaaggcga
     7081 agcgcaaagc cgccgaaaaa gcgcgcgata ccgccgccga gttgctcaac ctctacgccc
     7141 aacgcgccgc ccaatcggga cacaagtttg aaatcaacga gtcggactat caggcgtttg
     7201 ccgacggctt cggctacgag gaaaccgaag accaggccgc cgccatcgcc gccgtgatta
     7261 aagatttgac gcaggcgaag ccgatggacc gacttgtgtg cggcgatgtc ggtttcggca
     7321 aaaccgaagt cgccctgcgc gccgcgtttg tggcggtgat gggcggcaaa caggtcgccg
     7381 tactcgcccc gaccacgctt ctggtcgagc agcacgcgca aaacttcgcc gaccgtttcg
     7441 ccgatttccc cgtaaaagtc gccagccttt cgcgtttcaa caacagcaaa gccaccaaag
     7501 ccacgctcga aggcatggcg gacggtactg tcgatatcgt tatcggtacg cacaaattgg
     7561 tgcaggacga cataaaattc aaaaacttag gtttattgat tatcgatgaa gaacaccgct
     7621 tcggcgtgcg acagaaagag cagctcaaac gcctgcgcgc caatgtcgac atcctaacca
     7681 tgaccgccac gccgattccg cgcaccctca gcatggcgct cgaaggtctg cgcgacttct
     7741 cgctgattac cactgcaccg agccgccgcc tcgccgtgaa aacctttgtc aaaccgttca
     7801 gcgaaggcag cgtgcgcgaa gccgtgttgc gcgaactcaa acgcggagga caggtatttt
     7861 tcctgcacaa tgaagtagat acgattgaga atatgcgcga gcggctggaa accctgctgc
     7921 ccgaagcccg tatcggcgtg gcgcacggac aactgcgcga gcgcgagctg gaacaagtca
     7981 tgcgcgactt tttgcagcaa agattcaatg tgttgctctg ttccaccatc atcgaaaccg
     8041 gcatcgacat ccccaacgcc aacaccatca tcatcaaccg cgccgacaaa ttcgggctgg
     8101 cccaactgca ccagcttcgc ggacgcgtcg gccgcagcca ccaccaagcc tacgcctacc
     8161 tgctcacgcc cgaatacatt accaaagacg cagaaaaacg cctcgacgcc atcgcggcgg
     8221 cagacgaact cggcgcaggt ttcaccctcg ccatgcagga tttggaaatc cgaggcgcag
     8281 gcgaaatcct cggcgaagga caatccggcg aaatgatgca ggtcggcttc acgctctaca
     8341 ccgaaatgct caaacaggcc gtgcgcgacc tcaaaaaagg ccgccaaccc gacctcgacg
     8401 caccgttggg cattaccacc gaaatcaaac tgcacagccc ggcattactg cctgaagact
     8461 actgccccga catccacgag cgcctcgtcc tctacaaacg cctcgccgta tgcgaaaccg
     8521 tgcagcaaat caacgccata cacgaagaac tcgtcgaccg cttcggcctg cccgaacaac
     8581 ccgtcaaaac cctcattgaa agccaccacc tgcggcttgc ggcaaaagaa ctgggtattg
     8641 atgccatcga tgccacaagt gaagccgtaa ccgttacctt cggcaaacac cactgtatcg
     8701 acccgacggg gattatcctg ctgattcaga cggataaaaa ataccggctg gcaggggcag
     8761 acaaattgag gtttgcagcg gaaatggaaa acatcgaggt caggatcaat accgtcaaaa
     8821 ccgtactaaa aaccctgcaa ggcaaacgcc tgcccaaagg aaattgatgt aacgatgccg
     8881 tctgaagaac accgttcaga cggcatacaa tcccccaatc ctcaccaaaa ggaacaatca
     8941 tgtcgatttt tcacccctat ttccaacaac tgtccaccga aggttttgac ggagaagaag
     9001 atgtcctctg ggaagacgag ctgaccgtca aaggcaatct tgtcgaggta ttcctgtagg
     9061 cagaaaatga aaacagcctg ccaaccgaaa cattggatgc atttgccgcc ttcctgtccg
     9121 aattggaaaa agccgacgat gctgcacgcg ccgcactgac ggaatatttg aaacgggaca
     9181 gccgctatat cgactttcac accgaagata cccaaacaag ccgtgatgcg gcaaaatttg
     9241 tccgcactat gcagttgatc tatatcagct tatgggcaaa aaatccggct tttgccgtta
     9301 tggattatat gcccgccaat atggaaagcg acgaaatcct tgccgtcaaa ctgcatttgg
     9361 acggcagcat tttctcaatc gattgggaga gttgagccgg attgccaatc ggcaaaaatg
     9421 aaatgccgtc tgaaaacagc ttccctgccc gaagccttgc cattatgaat ttgaaggaaa
     9481 ctccactata atacggcatt cagattttca gacggcatca cgcccgtcag accgcacaca
     9541 aaccaaaagg aaatacatgt tccgtactat acttggcgga aaaatccacc gcgccaccgt
     9601 aaccgaagcc gatttaaact acgtcggcag cattaccgtc gatcaagacc tgttagacgc
     9661 ggcaggcatc tgccccaacg aaaaagtcgc catcgtcaac aacaacaacg gcgaacgttt
     9721 tgaaacctat accattgcag ggaaacgcgg cagcggcgtg atttgcctga acggtgctgc
     9781 agccaggctg gtacagaaag gcgacatcgt catcattatg tcttatatcc aactttccga
     9841 accggaaatc gccgcacacg aacccaaagt cgtcttagtg gacggaaaca ataaaatccg
     9901 cgacatcatc tcctacgagc cgccgcacac cgtactgtaa tcccgcaaac ggacatcgat
     9961 tatggatatt aaaatcaacg acatcaccct cggcaacaat tcgcctttcg tcctattcgg
    10021 cggcatcaac gttttagaag atttggattc caccctccaa acctgtgcgc attacgtcga
    10081 agttacccgc aaactgggca tcccctatat ctttaaagcc tctttcgaca aggcaaaccg
    10141 ctcgtctatc cattcctatc gcggcgtagg cttggaagaa ggcttaaaga tttttgaaaa
    10201 agtcaaagca gagttcggca tccccgtcat taccgacgta cacgaacccc atcaatgcca
    10261 acccgtcgcc gaagtgtgcg atgtcatcca gcttcccgcc tttcttgcgc ggcagaccga
    10321 tttggtggcc gcaatggcgg aaacgggcaa tgttatcaac atcaaaaaac cccagttcct
    10381 cagcccttcg caaatgaaaa acatcgtgga aaaattccgc gaagccggca acgggaagct
    10441 gattttatgc gaacgcggca gcagcttcgg ctacgacaac ctcgttgtcg atatgctcgg
    10501 tttcggcgtg atgaaacaaa cctgcggcaa cctgccggtt attttcgacg ttacccattc
    10561 cctgcaaacc cgcgatgccg gttctgccgc atccggcggt cgtcgcgcac aggctttgga
    10621 tttggcactt gcaggcatgg caacccgcct tgccggcctg ttcctcgaat cgcaccccga
    10681 tccgaaactg gcaaaatgcg acggccccag cgcgctgccg ctacaccttt tagaaaattt
    10741 tttaatccgc atcaaagcat tggacgattt aatcaaatca caaccgattt taacaatcga
    10801 gtaacacggt ttcgccttat gatgcagact ttccgaaaaa tcagcctgta tgccgcaacc
    10861 ttgtggctcg gtatgcagat tatggcaggt tatatcgccg caccggtgct gttcaaaatg
    10921 ctgcccaaaa tgcaggcggg cgaaattgcc ggcgtattgt tcgacatcct ctcttggagc
    10981 gggcttgccg tttggggcac ggtactggct gccgcctttg ccgccctaac ccggcggcaa
    11041 accgccctgc tgcttttttt attgtccgcc cttgccgcca accaattttt ggttacaccc
    11101 gttatcgagg cactgaaata cgggcatgaa aattggctgt tgtcggttgc aggcggatcc
    11161 ttcggaatgt ggcacggtat ttccagcatg actttcatgg caaccgccct actttcagca
    11221 gttttaagtt ggcggctttc cggcaaagag gccgtctgaa gccctcccat tttttacctc
    11281 ccttcacttc acttggagaa cattcatgag cgcaatcgtt gatattttcg cccgcgaaat
    11341 tttggactcg cgcggcaacc ccacagtcga gtgtgatgta ttgctcgaat ccggcgtaat
    11401 gggacgtgcg gccgtaccga gcggcgcatc caccggtcag aaagaagctt tggaacttcg
    11461 cgacggcgac aaatcccgct attccggcaa aggcgtattg aaggcggtcg aacacgtcaa
    11521 caaccaaatc gcccaagccc tcatcggtat cgatgccaac gagcaatctt atatcgacca
    11581 aatcatgatc gaattggacg gtactgaaaa caaaggcaat ttgggtgcga atgcgacttt
    11641 ggcggtctct atggcggttg cacgcgccgc tgccgaagac tcaggcctgc cgctttaccg
    11701 ctacttgggc ggcgcaggtc cgatgtccct gcccgtaccg atgatgaacg tcatcaacgg
    11761 cggcgaacac gccaacaaca gcctgaacat ccaagagttt atgattatgc ccgtcggcgc
    11821 aaaatctttc cgcgaagcgt tgcgctgcgg tgcggaaatt ttccacgcct tgaaaaaact
    11881 gtgcgacagt aaaggcttcc cgaccacagt cggcgacgaa ggcggtttcg cccccaacct
    11941 gaacagccac aaagaagccc tgcaactgat ggtcgaagcg gccgaagccg ccggctacaa
    12001 ggcgggcgaa gacgtattat tcgcattgga ctgcgcgtcc agcgagttct acaaagacgg
    12061 caaataccac ttggaagccg aaggccgctc ctacaccaac gcggaatttg ccgaatactt
    12121 ggaaggcttg gttaacgaat tcccgattat ttccattgaa gacgggatgg acgaaaacga
    12181 ctgggaaggc tggaaactgc tgaccgaaaa attgggcaaa aaagttcaat tggtcggcga
    12241 cgacttgttc gtaaccaatc cgaaaattct tgccgaaggc atcgaaaaag gcgtagcaaa
    12301 cgcattgctg gtcaaagtca accaaatcgg tactttaagc gaaaccctga aagccgtcga
    12361 tctggcaaaa tgcaaccgct acgccagcgt gatgagccac cgctccggcg aaaccgaaga
    12421 cagtaccatt gccgacttgg cagtcgccac caactgtatg cagattaaaa ccggttcttt
    12481 gagccgttcc gaccgcatgg cgaaatacaa ccaactgctg cgtatcgagg aagaattggc
    12541 ggaagccgcc tactaccccg gcaaagccgc attctaccaa ctgggcaaat aaataaaaaa
    12601 gtggatgtat gtttcaacac acagggcaac acatgaagcg tcgccctgtg tgttgacctg
    12661 atttggaaga ggttacacct ctcccaaata aagtctaatt ttaccgccac gaaggacgga
    12721 catccgagtg gtggggtttc aaccagtaag gaaacgttga tgaagtgggt aactgtcgtt
    12781 ttatccttcg cacttgtctg ttgccaatac agcctgtggt tcggcaaagg cagcatcgga
    12841 cgcaacagca gtctgagaga acagattgcc gttcaagaag aaaaaaacca gacactcgcc
    12901 ctacgcaatc attcccttgc cgccgaagtc tatgatttgg aaaacggtca agaagccatt
    12961 tcggaaatcg cccgggtaga actgggttat atccaagacg gcgaaacctt ttaccgactc
    13021 atcaggcata accggtaata ccgtcaaaaa gccgtccgaa ccaatgttcg gacggctttt
    13081 atttcaacaa actgtcagac agcccctcat cctcccccgc caaaccgcaa tccagcctga
    13141 catccccctc gacgcaacag cagcacggca gtatctcgtc ccgccccaaa aaagccaaag
    13201 gcggctcccg ataagtaacg cttccctcca aaatctttac ccggcacgat ccgcaatatc
    13261 cgcttcggca ctgatattcc accatatgcc ccgtccgttc caagccttcc aacagggtct
    13321 caccctccaa gagctcaaaa aggcccttat tcgtaccaat gcgcgccatt tccgaccaat
    13381 caaaaataaa aggaacaata accgatccta cctgctgttt ttcccatcat acaacacaca
    13441 aatgccgtct gaaacatccg gcttcagacg gcattttttc aaaaaacatt tacaactcaa
    13501 aatcgcccaa gtcatccgta ttcacttcag aatcgatttg accaatcaaa taagaggata
    13561 tttccacttc ctgcggcgcg acctgtacgt tgtcggacga cagccacgca ttaatccacg
    13621 gaatcgggtt ttgatttgcg ccttcaaatc cggccgacag ccccaccgcc tgcatacgca
    13681 gattggtaat atattcgacg tattgggata agatttcttt gttcaaaccg atcatcgaac
    13741 cgtctttaaa caaatatgcc gcccattctt tttcctgttc cgccgctttt ttgaagagtt
    13801 ggaaacattc gtcctgcaac tcggcggcaa tttccgccat ttccggatca tcaacacccg
    13861 aacgcatcag attaagcata tgctgcgtgc cggtcaggtg cagggcttca tcgcgggcaa
    13921 tcagtttgat gattttggcg ttgccttcca tcaactcacg ctcagcaaaa gcaaacgagc
    13981 aggcaaatga aacgtagaaa cggatggctt ccaacacatt gacgcacatc aggcagagat
    14041 agagtttttt cttcaacccg cgcaaagaca cagtaacggg ttttccgccg acattgtgca
    14101 ccccttcgcc caacaggttg taatattggg tgtattcgat taaatcatca taatagcagg
    14161 caatgtcttc ggcgcgggcg gtaatgtatt cgttttcgac aatatcatca aacacgaccg
    14221 acggatcatt cacaatattg cggatgatgt gggtatagct gcgcgagtgg atggtttcgc
    14281 tgaagctcca cgtttcaacc caagtttcca gctcgggaat cgaaaccaaa ggcagcaagg
    14341 caacattcgg actgcgccct tggatggaat cgagcagtgt ttgatatttc agattgctga
    14401 tgaaaatatg tttttcgtgt tcgggcaggt tggcgtagtc gatacggtcg cgcgacacgt
    14461 cgatttcttc cggccgccaa aaaaacgaca attgtttttc aatcagtttt tcaaatacct
    14521 cgtatttctg ctggtcataa cgggcgacat ttaccggctg accgaaaaac atcggctctt
    14581 tcagcgcgtc gtttttggtt ttgggaaagg tgctgtatga catgactagg tgttcgcagg
    14641 acataacgtc tctcttttta aaaattagtc gacttttcta ttaattttta attcaccata
    14701 taaaacatgt tgaattctat caataaagtg ccatcctaaa atagcaaata ctaaaagaac
    14761 actaataaat atataaaatt tatattcaac taagaatgac cgaatgacat ccatactttt
    14821 ccttttctgg aaaataacaa cttagcctca tatagtagta ggcactaatt taagaaaatc
    14881 cttgaagcct cctactatct agaagccttg gatatatttc aaaaattaac ctgttttaaa
    14941 tcttacaagc cccaccagcg cagccgtcat cttgaatatc ggtctgcgtg tcgtccgcgc
    15001 cgtcgcgggt gttgtggtag tacagggttt tgacgccgta tttgtaggca gtcagcaggt
    15061 ctttgagcat ttgtttcata gaaactttgt taccttcgaa tttgcccggg tcgtaagcag
    15121 tgttggcgga aatcgcctga tcgacgaatt tttgcattac gccgacaagt ttcaggtagc
    15181 cttcgttgcc cggaagctgc cacagggttt cgtaggcatt tttcaaggtt tcaaactccg
    15241 gcacgacttg tttcaaaatg ccgtctttcg atgctttgac cgttaccaat ccgcgcggcg
    15301 gctcgatgcc gttggttgcg ttggcgattt gcgagctggt ttcagacggc atgagcgcgg
    15361 tcagagtaga gttgcgcagg ccgtatttga cgatgtcggc acgcaggctt tcccagtcgt
    15421 aatgcaaagg ctcgccgcag acggcatcca aatctttttt gtaggtgtcg atgggcagtt
    15481 tgccttgcga ataaacggtt tggttaaaga gcgtgcacgc tccgtattct ttggcaaggt
    15541 ttgccgatgc tttgagcagg taatactgta tggcttcaaa ggtacggtgg gtcagaccga
    15601 gcgcggaacc gtcgctgtag cggacaccgt ttttcgccag atagtaggcg tagttgatga
    15661 caccgatgcc gagcgaacgc cggtccatag tagaggtacg cgcggcttct accggatatc
    15721 cctgataatc taaaagtgca tcgagtgcgc gcacggtcaa atcggcaagc ccttccaatt
    15781 cgtccaagct gtttaatgca cccaagttga aggcggacag tgtacacagg gctatttcgc
    15841 cgtcagggtc gttgatgttg tccagcggtt tggtcggcag ggcgatttcc atacacaagt
    15901 tggactgatg aacaggcgca acgcgcggat cgaacgggct gtgcgtattg cagtgatcga
    15961 cgttttgaat gtagatgcgc ccggtcccgg cacgctcctg catcagcgtg gaaaacaggt
    16021 cggcagccgg aatgatacgc ttgcgtatgt tcgggtcttg ctcgtatttc gtatagagcc
    16081 gctcaaattc gtcttggtcg gcaaaaaacg cttcgtacaa ccccgaaacc tcgttgggcg
    16141 aaaacagcgt aatgttgccg cccttaatca ggcgggtgta cagcaggcgg ttgatttgca
    16201 cgccataatc aagctggcgg atacggttgt cttccacgcc ccggttgttt ttcaacacca
    16261 gcaggctttc ggcttcaata tgccacaagg ggtagaacaa ggttgccgcg ccgccgcgca
    16321 cgccaccttg cgaacaagat ttgaccgccg cctgaaacat cttaaagaag ggaatgcagc
    16381 cggtatgccg ggcttcaccg ccccggattt cgctgtccag cccgcggata cgtccggcat
    16441 tgatgccgat gcccgcgcgt tgggaaacgt atttcacaat cgcgctggta gtggcattga
    16501 tggaatccaa actatcgtcg cattcaatca gcacacagct tgagaactgg cgcgtaggcg
    16561 tacgcacgcc gctcataatc ggcgtgggca acgatacttt gaatgtggaa acggcatcgt
    16621 aaaaccgttt gacgtaaccc aagcgcgcct ctttcgggta tttgctgaaa aggcacatcg
    16681 ccaccaaaac atataaaaac tgcggcgttt cgtaaatctg gcgggtaacg cggttctgta
    16741 ccagatattt gccttcgagc tgtttgacgg cggcatagga aaaggacata tcgcgttcgt
    16801 ggtcgatata ggcgttcagt tcgtcaaatt cttcgcggct gtaatcctca aggatatgcc
    16861 tgtcgtattt tccggcatcg gtgatctttt taacatggtt gtaaaggtgc ggcggctcgt
    16921 attcgccgta ggctatttta cgaagatgga aaatcgccaa acgtgcggca aggtattggt
    16981 agtccggggt atcttccgaa attaaatctg cagcggcttt gatgatggtt tcgtggatgt
    17041 cgtcggtgcg gatgccgttg tagaactgga tgtgcgattt caactcgacc tgcgacacgg
    17101 aaacattttc caatccttcc gccgcccaag taacgacacg gtgaatctta tccaaatcaa
    17161 tggcttccaa tcttccgtct cgtttggtta cttttaaatc agtcggtgta ttcatcgctt
    17221 cctcttccac tcttgatatt caagacacgg tcttttcaaa taaattaagg cagacaatat
    17281 agtggatttt tagcattttc tccagtcttg acaacgattg catttttcag tttcaggcaa
    17341 tgcgcgataa aacccgcctg tcgtattttt cctataacat ttgttttatc tgataattct
    17401 ttaccgataa aaaacgggta aattttttgc cttttgaccg gctccggcta caaggcgatg
    17461 aaataaggat tttccgacga aaaaggaaaa cttccggttt tccgcccctg cgaattgtta
    17521 aattttgcaa agtatgattt ttgccacgcc gccgccgaca aattccattt tcttaccgat
    17581 tggaatttat tattgagatt aatatgttat ttgaatttgc atatcaaacg gcaagttttg
    17641 tcggctgaat gagtctgaaa ctgccgacag tttgcccgtt ccatccttcc atcctatact
    17701 ggaaggaatt acaaactgaa aggatcgtca tgtccgtcaa taaagatgct tgccatactg
    17761 ttgtctgcaa tactgggact ggtatcaaca actgccgctg ccggtacgtc agaacccgcc
    17821 caccgacata ccaaacatat cagcaaggca aacaagcaga tgctgcaccc cgaatgcagg
    17881 aaatatttgg aacgccgtgc cgcgtggtac cgatcgcaag gcaacgtgca ggaattgcgc
    17941 gaaaacaaaa aggcgcgcaa agcattccgc accctgcctt atgcggaaca gaaaatccaa
    18001 tgccgggcgg cttatgaggc tttcgatgat ttcgacggcg gcaggttccg ccgttaatcc
    18061 catataaaat atgctgtctg aacacaagtt aagatggcat tttccataga tatagtggat
    18121 taaattta
//
LOCUS       DAXDXW010000047        18056 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXDXW010000047 DAXDXW010000000
VERSION     DAXDXW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18056
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.048"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..1253
                     /locus_tag="ACFB64_001315"
     CDS             <1..1253
                     /locus_tag="ACFB64_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFC1209669.1"
                     /translation="KYAVALGSSSTASGEYSYASGYNSVASGNKSYAAGYASVASAEG
                     SVVIGDSRQVKPEADQGVAVGSKATVKNKAKQRVVVGSEAKVNAERGIAIGKEAKAGG
                     KTTNTLLDGPAYYADAIAVGYQAEAGKGGAIALGKQAKATKQNGMALGVESEAAGDFS
                     TAVGNESKAKGQGGVGLGNQSKAEADFAVAVGNKAEATKENSLVIGRYARANGNHSVS
                     LGSRSEIKDGVSNSVAPGYGSVASENNVVSVAYKETPQSTELSYRKIVGVDDGVNDFD
                     AVNVRQLKAMQGQNMAELFSVRSEVRGVAASSAALSALTPLSYDANNPTQFMVGFGTY
                     KGRQAMALGLSHFVNDRFMVKVGGTLGGNKTGHMMNVGLTWKFGTSQGVPATSDKVLM
                     EQLMQDNRQLKARLEKLEARLNAM"
     gene            complement(1392..1907)
                     /gene="coaD"
                     /locus_tag="ACFB64_001316"
     CDS             complement(1392..1907)
                     /gene="coaD"
                     /locus_tag="ACFB64_001316"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC1209670.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRNTYTAAERQDMLCAITDNFPNVRIEVFQNRFLVHYAREVDAGFIVRGIRSTSDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMIKGLVGPEGWMETVKRYVPPAVYQ
                     KMIAEHHNNNA"
     gene            complement(2037..2720)
                     /locus_tag="ACFB64_001317"
     CDS             complement(2037..2720)
                     /locus_tag="ACFB64_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFC1209671.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSYISAALTLFISLYNIFSSLLN
                     ILLSLNGDD"
     gene            complement(2877..3143)
                     /locus_tag="ACFB64_001318"
     CDS             complement(2877..3143)
                     /locus_tag="ACFB64_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC1209672.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(3217..3687)
                     /gene="rlmH"
                     /locus_tag="ACFB64_001319"
     CDS             complement(3217..3687)
                     /gene="rlmH"
                     /locus_tag="ACFB64_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC1209673.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDAAYALKEIKPEKRG
                     AGVNAVQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAVSILHNHPYHRE"
     gene            complement(3740..4126)
                     /gene="rsfS"
                     /locus_tag="ACFB64_001320"
     CDS             complement(3740..4126)
                     /gene="rsfS"
                     /locus_tag="ACFB64_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC1209674.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTIWGGEKPSFHAGMQKPWHAAD"
     gene            complement(4185..4790)
                     /gene="nadD"
                     /locus_tag="ACFB64_001321"
     CDS             complement(4185..4790)
                     /gene="nadD"
                     /locus_tag="ACFB64_001321"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC1209675.1"
                     /translation="MKKIGLFGGTFDPIHNGHFHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVRESATYTFDTVQIFRRQFPSAQLWW
                     LMGSDSLLKLHTWKKWQLLVRETNIAVAMRQGDSLHQTPRELHAWLGNALQDGSVRIL
                     SAPMHNVSSTEIRRNLSAAGVSDGIPPVAARYIRKHGLYEK"
     gene            complement(4787..5386)
                     /locus_tag="ACFB64_001322"
     CDS             complement(4787..5386)
                     /locus_tag="ACFB64_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC1209676.1"
                     /translation="MSELSEILSYNQKFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGVDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            complement(5415..6956)
                     /locus_tag="ACFB64_001323"
     CDS             complement(5415..6956)
                     /locus_tag="ACFB64_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC1209677.1"
                     /translation="MDGRCWAVRGAFSLLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFAQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAGASALVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFSVAVLSVCCLFPLSAIVVKAWSAGESRRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRLVWMRGLVFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDNGEGGK
                     RTETL"
     gene            7347..8733
                     /locus_tag="ACFB64_001324"
                     /pseudo
     CDS             7347..8733
                     /locus_tag="ACFB64_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
     gene            8753..9271
                     /locus_tag="ACFB64_001325"
     CDS             8753..9271
                     /locus_tag="ACFB64_001325"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC1209678.1"
                     /translation="MTTHFVVTGVCGCGKTTAAPSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGADHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(9457..10374)
                     /gene="thrB"
                     /locus_tag="ACFB64_001326"
     CDS             complement(9457..10374)
                     /gene="thrB"
                     /locus_tag="ACFB64_001326"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC1209679.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELSFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAGEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(10411..11139)
                     /gene="ubiG"
                     /locus_tag="ACFB64_001327"
     CDS             complement(10411..11139)
                     /gene="ubiG"
                     /locus_tag="ACFB64_001327"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC1209680.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLRGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECVRVEDLAGAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVRPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            11287..12528
                     /locus_tag="ACFB64_001328"
     CDS             11287..12528
                     /locus_tag="ACFB64_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC1209681.1"
                     /translation="MSAKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSGGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGSAVGGKISLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVLTF
                     IWATGGLVADAKPSVLFDTQAPAGTGYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWAGTLVALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSMSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGCVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIANIAAQVLSQMELVPVFKG"
     gene            12680..13204
                     /locus_tag="ACFB64_001329"
     CDS             12680..13204
                     /locus_tag="ACFB64_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC1209682.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQRRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIVAHSHMMRRELVGLYGVPPERIQ
                     VAPPRRYGTLLSTT"
     gene            13218..13733
                     /locus_tag="ACFB64_001330"
     CDS             13218..13733
                     /locus_tag="ACFB64_001330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC1209683.1"
                     /translation="MRAKYGFADHETVFLFPSTGHTRKGLELLADFFEQTELPVKLAV
                     AGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVESALCGTRVVLSE
                     NMACTEVMNEEAGFFFSRQNPETLAQAVARAVSLKKQGGHRLSDPMRALNYNPSLSHH
                     IDRLTDMLASV"
     gene            complement(13800..15452)
                     /locus_tag="ACFB64_001331"
     CDS             complement(13800..15452)
                     /locus_tag="ACFB64_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFC1209684.1"
                     /translation="MVAYAFLFLFVTAAVLLIVRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLGMPWRWAGAVLFAAAFVSVRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETRPYGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQDFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNRELVGNYRNYLYDVLKIAR"
     gene            15595..16158
                     /gene="gmhB"
                     /locus_tag="ACFB64_001332"
     CDS             15595..16158
                     /gene="gmhB"
                     /locus_tag="ACFB64_001332"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC1209685.1"
                     /translation="MKLIILDRDGVINRDRDDFVKSVDEWIPVEGSMDAVAFLTQAGC
                     TVAVATNQSGIGRKYFTVQDLTEMHAKMHRLIRQAGGEIDGIWFCPHTGADGCNCRKP
                     KPGMIEDILERFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PGHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            16188..16931
                     /locus_tag="ACFB64_001333"
     CDS             16188..16931
                     /locus_tag="ACFB64_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC1209686.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVGI
                     LNWSLKHIVGLKYRIIGAEHIPDRPSVICAKHQSGWETLALQEIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVIICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAEMPSETA"
     gene            16928..17617
                     /locus_tag="ACFB64_001334"
     CDS             16928..17617
                     /locus_tag="ACFB64_001334"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC1209687.1"
                     /translation="MTSLIHTLSDGIELTVEINRRAKKNLIIRPIGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRRTLAKTPPHNTANRLPEHIWFHGRQLALTAHQDTQILLMPS
                     EILVPEGAPEKQLALLRDFLERQAHSYLIPRLEHHARATQLFPASSSLTSAKTFWGVC
                     RKTTGIRLNRRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPEAKQRI
                     KIHGRELFALG"
     gene            complement(17681..17872)
                     /locus_tag="ACFB64_001335"
     CDS             complement(17681..17872)
                     /locus_tag="ACFB64_001335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209688.1"
                     /translation="MPSENLSDGIFAGKSVEDFAPAQAFHTARRIGRVSEGRCEMKRC
                     GRRGCRHPAPNRRQSVIAV"
BASE COUNT         4292 a         5459 c         4597 g         3708 t
ORIGIN      
        1 agaaatatgc agtagcatta ggatctagtt ctactgccag tggagagtat tcctatgcgt
       61 cgggctataa ttctgttgcc agtggaaata aatcctatgc tgcaggctat gcttctgttg
      121 ccagtgcaga aggcagtgtt gtaattggcg attcaaggca agtaaaaccg gaagcagatc
      181 aaggggttgc agtaggttct aaagcaacgg taaagaacaa agctaaacaa agggttgtgg
      241 taggttctga ggcaaaggta aatgcagaac gtggtattgc gattggtaaa gaagccaaag
      301 cagggggcaa aacaacgaac acactgttag atggaccggc atattatgcg gacgctattg
      361 ccgtcgggta tcaagcagag gctggcaaag gtggcgccat cgctttggga aaacaagcaa
      421 aagcaacgaa gcaaaatgga atggcacttg gcgtagaatc agaagctgcc ggtgatttta
      481 gtaccgccgt gggtaatgaa agcaaggcaa aggggcaagg cggcgtaggt ctcggtaacc
      541 aatctaaggc agaggcagat tttgcggtgg ctgtggggaa taaggctgaa gcgactaagg
      601 aaaactcact tgtcattggc cgatatgcaa gggcaaacgg aaatcattct gtgagtttag
      661 gatctcgtag tgaaataaaa gatggtgtga gcaattctgt ggcaccgggt tatggttcag
      721 tagctagtga gaataatgtc gtctctgttg cctataaaga aacaccacaa tctacagaac
      781 tttcctatag aaaaattgta ggtgttgatg atggtgtcaa tgattttgat gccgtcaatg
      841 tacgccagtt gaaagcgatg caaggacaaa atatggctga gctattttca gtacgcagcg
      901 aagtgcgtgg tgtggcagcg agtagtgcag ccctatcggc tctgactcca ttatcctatg
      961 atgcaaacaa tcctacgcag tttatggtcg gtttcggcac atacaaagga cgtcaagcta
     1021 tggcattagg attgtcccat ttcgtcaatg accgctttat ggtaaaagta ggcggaacct
     1081 taggcggcaa taagacggga catatgatga atgtaggctt gacttggaaa tttggaacga
     1141 gccaaggtgt tccggctaca agtgataagg tgcttatgga acagttgatg caagataatc
     1201 gtcaattaaa ggcacgattg gagaaattag aagcacgtct gaatgctatg tagccttgtg
     1261 gcgataacgg tttattgagt atatcgccaa tgataaagtg cataattttc aaaccgttga
     1321 taatatacaa taaattgtaa ccaatacctc aaacgggttt gaacccgaat atgcctgaca
     1381 ggaactttct gtcaggcatt gttgttgtga tgttctgcaa tcattttttg gtacacggcc
     1441 ggcggcacat atcttttgac ggtttccatc cagccctcgg gcccgaccag tcctttgatc
     1501 atagtggacg acacttcggc gatttcgcgc ggcggcatga ggaatacggt agatatttcg
     1561 ggtgcgaggt cgctgttgat atggcgcatg gagcgttcgt attcataatc ggaggtggaa
     1621 cggatgccgc gcacgatgaa tcccgcatct acctcacggg cgtaatgcac caaaaaccgg
     1681 ttttgaaaca cttcaattct gacgtttggg aagttgtcgg taatggcgca caacatatcc
     1741 tgcctttcag cggcggtata ggtgttgcgt ttgtcgggat tgatgccgat ggcgacgatg
     1801 agttcgtcaa acatagattg cgcctgccgt atcatccaca gatgccccaa tgtgggcgga
     1861 tcgaaactgc cggcataaac ggcgcggcgc ggtgtattcg gtaacatttg attcctcccg
     1921 gcttcatagc cggctgtgtt tttatgtgtt tgcatccgta ttgtatgccc aaaacaaaat
     1981 gccgtctgaa aataagtgct ttcagacggc atggtttgca tggtgtttcg cctacatcag
     2041 tcgtcgccgt ttaaggacag caggatgttg agcaggctgc tgaagatgtt gtaaagcgag
     2101 ataaacagtg tcagtgccgc gctgatgtaa ctgtcttcgc cgccgtcgat gacggtgcgt
     2161 acctgccaca taatcattaa ggaactgaac aagacaaaac cggcggaaat ggtcagggcg
     2221 agtgcgggaa tacccaaaaa cagattggca accacggcga ccatcagaat gaccgcacct
     2281 acggtcagga agcgtccgag cgcgttcata tcgagccggg ttcggcgtgc caatgcggac
     2341 atcgttaaaa agacggcggc ggtcatcgcg gcggcaatgc cgacgatttt cgcaccgtcg
     2401 gcaatatgga gtgcgtattg cagcacgggg ccgatcaata cgcccatacc gaatgtgaat
     2461 accatcagca gggtaacgcc ggtattgctg taacggtttt tttcgatgaa gtggatcata
     2521 ccgtagaaaa acgccaatac gacggcaaat cctatccagc gcgaaccgaa ggcggcgtaa
     2581 aaattgaaac cggcattggc ggcaagtgcc gcgcctgcgg ctgccggaat gaatgaaaat
     2641 ccgagcaggc ggtaggtttt ctgcaggacg gtgtttttag aaaccgtatg cgcggtgtag
     2701 tcgtaaacgt cgtgttgcat atcatctgct cctgaaagcg cggttggaaa taacggggga
     2761 ttttaacatt gcccaatgtc aaaatttgtc cggttgcgtg aagataaagt tgtccggcgt
     2821 attttaaagg ccgtctgaag cagtttcgga cagcctgtgt tcaaaacgga aaaccgttat
     2881 tgcggaacgt atccctgcac ggcatccgcg ccgtcgccga agaaatactg ctccatctgc
     2941 tgagccaggt attcgcgcgc gcgcggatcg gcaaggctta agcggttttc gttaatcagc
     3001 atggtttggt ggcgcgtcca cgccgcccat gcttcttgcg atacgttttc aaaaatgcgt
     3061 ttgcccaatt cgttgggcag cggcggaaat ttcatgcctt cggcttcttt gttgagcttg
     3121 acgcagaata ccattcgtgc catagcggat tcctttgctg tgttcagaaa taacggggcg
     3181 attttaaccg attagggggt acagacaaaa gcctttttat tcccgatgat aaggatggtt
     3241 gtgcaggatg gaaacggccc ggtagagctg ctcggtcaga aggacgcgca ccatgccgtg
     3301 cggcagggtg aggctggaca ggcgcatcat catgcgggct tgttgtttga ggcggtcggt
     3361 catgccgtcc gcgccgccga tgacgaagca gacgtgttcg ccgttttgcc gccagctttt
     3421 gaggtgttcc gccagctcga cggaggtcgg tgctttgccg cgttcgtcaa gaacgacgag
     3481 gaacgcgcct tgcggaatgg cttcaaggat gcgtttttct tccgccgcca taccttggac
     3541 ggcattcacg cccgcgccgc gtttttcggg tttgatttct ttgagtgcgt aggcggcgtc
     3601 gcgtccgaag cgtttggcgt attcggcgac ggcctcatca acccaacgcg gcattttggt
     3661 gccgactgcc aagacggtaa tgttcaatgc tttccccctt acaggaaatg ccgtatgaat
     3721 attcagacgg catcgggaat cagtctgcag cgtgccacgg cttctgcatt ccggcgtgga
     3781 aactcggttt ctcgccgccc cagatggtgt caatgtcgta gaagtcgcgc acggcaggga
     3841 gcatgacgtg gacgacgagg tctcctgcat caaccaacgt ccattcgccg ctgtctcctt
     3901 cggtactgag gatttcaaaa ccggcttctt tcaaatcgac ggcaacgttg ttggccagtg
     3961 ctttgacttg gcgcgtactg tcgccgctgg cgataatcat tctggcaaac agcgaagttt
     4021 tgtcttgcgt ttcgagaacg gaaatgtctt tggctttgat gtcttcgagg gcattgacgg
     4081 cgaccccgac cattttttgc aggtcttgca gttcttgttc gttcattgtt ttcctaacgg
     4141 gatgttttca gacggcatta tagcctctcc ttactgattt gactttattt ttcatacaaa
     4201 ccgtgttttc ggatataacg ggcggcgaca ggcgggatgc cgtctgaaac accggcggca
     4261 ctcaggttgc ggcggatttc cgttgacgac acattatgca tcggggctga caagatgcgg
     4321 acgctgccgt cctgaagggc attgcccagc cacgcgtgca gttcgcgcgg ggtttggtgc
     4381 aggctgtcgc cctgccgcat ggcgacggcg atattggttt cgcgcacgag caactgccat
     4441 tttttccaag tatgcagctt cagcaggctg tcgctgccca tcagccacca aagttgcgcg
     4501 gacgggaact gccggcggaa gatttggacg gtatcaaaag tataggttgc actttctcgg
     4561 acgatgtcgc agtcgctgac ggcaaaacgc gcgtcttctg ccgtcgccag ttcgaccatg
     4621 gcaaggcggt cggcggcgga agcggaggcg gcgtctttgt gatacgggcc gcctgccggc
     4681 aggaaaacaa ccgcgtccaa cccgatttcg tcggcaaagg cacgggcgat atgaaaatgc
     4741 ccgttgtgta tcgggtcgaa agtgccgccg aacagtccga ttttcttcat gatgtttcca
     4801 ttccttccga taagtccatg ccgtctgaaa cactgccgtc cacaaccagc gtcagtttgc
     4861 cggtaacggg atggatgacc agtccgtgaa cggcgatatg gcgcggcatc agcggatggt
     4921 tgcggataag gtccaccgtg tggcgcacgc tgtcttcgac gttgtcgaaa ccggtcagcc
     4981 agccgtcgag gtcgacacct gcataacgca gggtttcgat acggtcttcg ggaatccggc
     5041 tttcccggac gcgcccgagg aattcttcgg cattcagccc ctgcataccg caatcgtgat
     5101 gggcgatgac cataatctct ctgaccttca attcaaacac ggcaaccaaa aggctccgca
     5161 tcaccgaacc ccacgggtgc gtaaccagcg cgccggcatt tttaatcagc ttggcatcgc
     5221 cgtttttcaa acccaacgcg tcgggcagca gcccgataat ccgcgcatcc atacaggaca
     5281 aaaccgccag cccgcgttcg gggtatttgt cggtaaagta tttttcatat tctcccgact
     5341 cgacaaactt ctgattatag gaaaggattt cgctcaactc gctcattttg ccgtcctctg
     5401 aaaaagggtt cgcattataa cgtttccgtc cgttttccgc cttcgccgtt gtccaacagc
     5461 aggaaaatgc acaccgcaaa tgccgacaac agcaatgtca acaccattgc ccgcgcataa
     5521 ttgtcctcac ccgcacgccc caaataggca taaatcaaag tcgtcaacgt ctgccattcc
     5581 ggacgcgaca ggaacaaggt tgccgcaaat tcgcccacac acgtcgccgc cgccaaagtc
     5641 agaccgcgcc gcaacgccgg tttcaagagg gggaacgtga tacggcatgc cgtctgaaag
     5701 ccgtttgcgc ccaaacctgc cgccgccctg ccgtaatccg gcggcagtgc atcccaggcc
     5761 gataaaacat cttttgccac aaacggatac gccagcagcg catacatcgc cagcagcagc
     5821 ggtaacgaag ccgtccaccc cggataaagc agcagcacgc ccgccgaaac acaaaccggc
     5881 gacaccataa acggtaaaaa caccagtccg cgcatccaca ccagccgccg cgccgccgcc
     5941 gcatacacca cacccaaaac cgccgccgca aacaccgccg ccgccgaaaa gcgcaaagta
     6001 ttccacactg cctgccacgt ttcactttcc attaacacac gccgcgattc gccggccgac
     6061 cacgctttca caacaattgc cgacaaagga aacaggcagc acacggacaa caccgccacc
     6121 gaaaatgcca gcaatacata ttcccccacc gattgaggcg gcgacggcat cacgggggaa
     6181 accgccttat ccgaaaccgc gcgcctgccg aaccacgcat acagcaaccc tgccgccgcc
     6241 gttaccccca acaccagcca caccagcgcc gaagcccccg ccatatcgag ttcgaacata
     6301 accaactggt aaatttccac ttcgaccgtg gcataacggc tgccgcccaa cagcaatgcc
     6361 agcccgaacc ccgaaaaaca atacaggaag acaaggcaca cgccgccggc aagccacggg
     6421 cgcaaaacgg gcatttcaat gtcccaaaac cgccgccacg cccccgcgcc caacgtccgt
     6481 gccgtctgaa gccgtgccgc aggcacttga gcaaacccct gatacgccgc cctgaccaac
     6541 acgggcaggt tgaaaaacac attgccgtac aacaacagat acggcgtatc ctgccggccg
     6601 cgccacaaca gcccgtccgc cccgaacaga gccagcacgc ccacgcccgc caccagcgtc
     6661 ggcatcacaa acggcagcat cagcaggcgc agcaccaaag cccgccccgg gaacgccagc
     6721 cgcgccagca cccacgcgac aggcacgccc aaaggcagca ccagcacaca ggttgccgcc
     6781 gcctgaaaca ccgtccacgc caaacgtttg agcatatagg catccgacag caccgcgcgc
     6841 cacgccaaac cgtcatacgc cgccaccgcc cacaaaggcg caacgaccat taccgccaaa
     6901 aaagccgaag gcagcaggga aaaagcaccc cgtaccgccc aacaccgtcc atccatcgcc
     6961 ttccccactt gaaacactga tgttgcgatt gtacccaaaa gccccacata ccgtatattt
     7021 caatccgact acgtaccgta tccgccttcc tcccgccgtc tgaaatatag cggattaaca
     7081 aaaatcagga caaggcggcg ggccgcaggc agtacaaata gtacggaacc ggttcgcccg
     7141 gtgcttcagc gccttaggga accgttccct ttgagccaag gcggggcaac gccgtaccgg
     7201 tttttgttaa tccactataa atcgttcaaa taaacaggaa tataacttca gacaaacaac
     7261 ttaccgcccg atttgtgcta tcgttttcgc acaacttaaa aaaacctgac aattttgtac
     7321 ttttattaca gagaaaggct ttacaaatgg acggccggac acagacgctg tccgcgcaaa
     7381 ccttgttggg catttcggcg gcggcaatca tcctcattct gattttaatc gtcaaattcc
     7441 gcatccgcgc gctgctgaca ctggtcatcg ccagcctgct gacggctttg gcaaccggtt
     7501 tgcccacagg cagcatcgtc aacgacgtac tggtcaaaaa cttcggcggc acgctcggcg
     7561 gcgtggcgct tctggtcggt ctgggcgcaa tgctcggacg tttggtagaa acatccggcg
     7621 gcgcacagtc gctggcggac gcgctgatcc ggatgttcgg cgaaaaacgc gcaccgttcg
     7681 cgccgggcgt tgcctcgctg attttcggct tcccgatttt cttcgatgcc ggactgatcg
     7741 tcatgctgcc catcgtattc gccaccgcac ggcgcatgaa acaggacgta ctgcccttcg
     7801 cgcttgcctc cgtcggcgca tttatccgtc atgcacgtct tcctgccgcc ccatccgggc
     7861 ccgattgccg cttccgaatt ttacggcgcg aacatcggcc aggttttgat tttgggtctg
     7921 ccgaccgcct tcatcacatg gtatttcagc ggctatatgc tcggcaaagt gttggggcgc
     7981 gccatccatg ttcccgttcc cgaactgctc agcggcggca cgcgagacag cgacccgccg
     8041 aaagaacctg ccaaagcagg aacggtcgtc gccgtcatgc tgattcccat gctgctgatt
     8101 ttcctgaata ccggcgtatc ggccctcatc agcgaaaaac tcgtgagcgc ggacgaaacc
     8161 tgggttcaga cggcaaaaat gatcggttcg acacctgtcg cccttctgat ttccgtattg
     8221 gccgcactgt tggtcttggg acgcaaacgc ggcgaaagcg gcagcacgtt ggaaaaaacc
     8281 gtggacggcg cactcgcccc cgtctgttcc gtgattctga ttaccggcgc gggcggtatg
     8341 ttcggcggcg ttttgcgcgc ttccggcatc ggcaaggcac tcgccgacag catggcggat
     8401 ttgggcattc ccgtcctttt gggctgcttc cttgccgcct tggcactgcg tatcgcgcaa
     8461 ggttcggcaa ccgtcgccct gaccaccgcc gccgcgctga tggctcctgc cgttgccgcc
     8521 gccggcttta ccgactggca gctcgcctgt atcgtattgg caacggcggc aggttcggtc
     8581 ggttgcagcc acttcaacga ctccggcttc tggctggtcg gccgcctctt ggatatggac
     8641 gtaccgacca cgctgaaaac ctggacggcc aaccaaaccc tcatcgcact catcggcttt
     8701 gccttgtccg cactgctgtt tgccatcgtc tgacagacgg aaaggatagt aaatgactac
     8761 gcattttgtc gttacgggcg tatgcggctg cggcaagacc accgccgcgc cgtccctgca
     8821 gaaacacctc ggtcaatgtc cctatgccga aggcgacgag ttccacaccc aagccaaccg
     8881 cgacaagatg ggcgcgggta ttccgctgac cgatgaagac cgctatccgt ggttgggcaa
     8941 tctgcgcgac tggatgacgc aacaggcgca aaacggcgcg gaccacacca tcgtaacctg
     9001 ttccgccctc aaacgcggct accgcgacat tctgcgcgga gccgaaggca aagccgcctt
     9061 catccacctc agtccgccgc aagacatcaa cctcgaacgc atgatgtcgc gcaaagggca
     9121 ctacatgaaa gcagggatgc tcgattcgca actggaaatc ctcgaggaac tgggcgaagg
     9181 cgaatacggg gtcaaaatcg ccaaccccgg cacgcccgaa gcggtcgaag ccgatattct
     9241 gaactgggtt gcctcggaaa acctgctttg aagcaatatg ccgtctgaag cccggcacag
     9301 gatgggtttc agacggcata aacatcggga acagaatgaa ttacattgat ttaaaatgga
     9361 aaatacccgg ctatcgtctc attttcgttt taatcagcca taaaaatgcc gtccgaaacc
     9421 ctttcagacg gcatttctgt caaacgccgg acgcactcaa tccaaactca acagcaggtt
     9481 gcggaacgcg ttcgggtctt tgataaacgt catctcgccc gcctgcggaa aatgaaaatc
     9541 caacaggcgc gacacccaaa aacggatgca gccggcgcgt tgggcggtcg ggaaatacgc
     9601 cttttccccg gcactcaagg ggcgcacgcc ctcataaccg ccgataaacg cctttttcaa
     9661 cgcctcatcc aacttattgt ccgccgtcct tgcccaatcg ttgaccgcaa tcgccaagtc
     9721 atacataaaa ttgccccggc aggcataata gaaatcgatg aagcccgata cctgaccgcc
     9781 gtcaagcaac acattgtctt taaacaggtc ggcgtggatg atgcccgaag gcagatggtt
     9841 gccgagattg tccttcagcg catcgatttc ggaacacagc agcgcggcat cgtcttgcga
     9901 caggacgggc agcagccggg cgcacgcttc cgtccaccac gcatcgtaac gcgggttttc
     9961 catttccaaa gggaaatcgg cggcggcaag gtgcattttc gccaacatcg caccggtatg
    10021 gaaacactgc tcagccgtcg gcagcgcggt atccgaacct ttcaggcagg caaccaggca
    10081 ggccggcttg cccgccaaaa cggaatcgag ccggccgtct ttgcgcgcaa ccggcgcggc
    10141 aaccgccacg cccttcatac tcaaatgccg gttaagctcc agaaaaaacg acagctcttc
    10201 ctgtttcaac acttcaaaca cggtcagcac ataacgtccc gaagtcgtcg tcagaaaata
    10261 attgctgttg gtaatcccct gcgcaatgcc ctgcagggaa acaaattccc ccaaatcgta
    10321 accgctcagg aagccgcgca tttcatcatc ggaaacactg gtatagacag acatatcaac
    10381 ttacttgctc aaaaacgggc aaacccgccg tcagaacgcc ggacggcagg caaacatata
    10441 attcacatcg gtcgaatcgc acagggcata agtttgcgac aacacatggt aagtcatacc
    10501 cttcgtatcc gccacatcca agcccgcctg acggcacatc cgcgccagct cggcaggcgc
    10561 gatgaatttt ttccagtcgt gcgtaccttt ggggacaaac ttcaacagat attccgccgc
    10621 cacaatcaga tgcaggtacg atttcgggtt tttattgatg gtggaaaaaa acaccatgcc
    10681 gtccggcctg accagcttgg cacaggcacg cacgatggcg gcgggatcgg ggacgtgttc
    10741 catcatttcc atgcacgtta ccacatcgaa cgagtgcggt tccgccccgg caaggtcttc
    10801 cacgcggaca cattcgtatt cgatatcggc gacattgttc aaagccgcgt gcaggcgggc
    10861 ggtttccaac gactgctccg ccatgtcgat acctttgaca aacgccgcgc cgcgccgcgc
    10921 catgctttcc gcgaggatgc cgccgccaca gcctacatct aaaacccgtt tgccgcgcaa
    10981 atccgcgtgt ccgtcgatat aatccagccg cagcggattg atgtcgtgca aggttttgaa
    11041 ctcgcccgac ttgtcccacc atttgtcggc aatccggctg aacttggcga tttccccctc
    11101 atcgacatta tattttttgt cggacatttt ccctcccatc tgacgaaccg ccccactcaa
    11161 aaacccaaga ttatactatg ggacgcaatg ccgtctgaaa gccttttcgg gcggcgcggc
    11221 gcaaatacct tacaaaccct tacactttac ggcataatgc aggctgtttt tcccgaggag
    11281 gcttatatgt ccgccaaaac tccgtcgctc ttcggcggcg cgatgattat cgccggtacg
    11341 gtcatcggcg caggtatgct cgccaacccc accgccacct ccggcgtatg gtttaccggc
    11401 tcgctggccg tgttgctgta cacctggttt tccatgctct ccggcggcct gatgattttg
    11461 gaagtcaaca cccattatcc gcacggcgca agtttcgaca cgatggtcaa agacctgctc
    11521 ggacgcggct ggaacatcat caacggcatc gccgtcgcct tcgttttata cctgctcacc
    11581 tacgcctaca tcttcgtcgg cggcgacctg accgccaaag gcatcggcag cgcagtaggc
    11641 ggcaaaattt cgctcaccgt cggacaactc gtcttcttcg gcatcctcgc cttttgcgta
    11701 tgggcatccg cacgcttggt cgaccgcttt accggcgtcc tcatcggcgg catggtatta
    11761 acctttattt gggcaaccgg cggcctggtt gccgatgcca aaccgtccgt cctcttcgac
    11821 acccaagccc ccgccggcac cggctactgg atttacgccg ccaccgccct gcccgtctgc
    11881 ctcgcttcct tcggcttcca cggcaacgtt tccagcctgc tcaaatactt taaaggcgac
    11941 gcgcccaaag tggcgaaatc catctgggca ggtacgttgg ttgccttggt aatttacgtc
    12001 ctctggcaaa ccgccatcca aggcaacctg ccgcgcaacg agttcgcccc cgtgattgcc
    12061 gccgaagggc aagtctccgt cctgattgaa accctgtcca aattcgccca aaccggtaat
    12121 atggataaaa tattgtccct attttcctac atggcaatcg ccacctcgtt tttaggcgta
    12181 accttaggcc tgttcgacta catcgccgac atcttcaaat ggaacgacag catgtccggc
    12241 cgcaccaaaa ccgccgcgct gaccttcctg ccgcccctga tttcctgcct gctcttcccc
    12301 accggcttcg ttaccgccat cggctgcgtc ggcctggcgg caaccgtctg gacaggcatc
    12361 atccccgcca tgctgctcta ccgttcgcgc aaaaaattcg gcgcaggcaa aacctataaa
    12421 gtttacggcg gcttgtggct gatggtttgg gtcttccttt tcggcatcgc caacatcgcc
    12481 gcacaggtat tgagccaaat ggaactcgtc cccgtattta aaggataaag gcaaaatgcc
    12541 gtttgaaacc cgccggcggc ttcaggcggc attgccgcaa caaacggcaa ccgtattccg
    12601 gcacacagcg cattacccta ccccctcacg cacaaatccc gccccgacag gcgcgggacg
    12661 caaccataaa ggaacaatga tgaagctcaa aatagacatt gcaaccaaca acttcaaaca
    12721 cggcggcggc acggaacgct acacattgga tttggtaaag ggtctgaaca gacaaaacat
    12781 cacaccggcc gtttatgcga cgaaatttga tcacggcatt cccgaatacg ccatgatcga
    12841 accccatctt gtcgatcaac gccggacgct gaaaaaacta cgctcgttcc tcttttcaag
    12901 ccggctcgct caaaccagaa aaaacagtgc cgccaaactg attgcctgcc accacgccga
    12961 ttacgccgac ctcctcatct gcggcggcac gcacttgggc tacctgcacc atatggcgca
    13021 aaaaccgaac ctgctcgacc gcctcgccat acgccgcaac cgcagcaact acgccaccgc
    13081 caaactgatt gtggcgcatt cccatatgat gcggcgcgaa ctggtcggac tatacggcgt
    13141 tccccccgaa agaatccaag tcgccccccc ccgcagatac ggaacgcttc tttccacaac
    13201 ctagagaaac tgccgccctg cgcgccaaat acggttttgc cgaccatgaa accgttttcc
    13261 tattcccatc gaccggccac acgcgcaaag gcctggaact gctcgccgac tttttcgaac
    13321 aaaccgaact gcccgtcaag ctcgccgttg ccggttcacc gcttccccgc ccgatgaaaa
    13381 acgtcgtcgg actgggcttc tgcaccgata tgcccgaact ctaccgcgcc gccgacttta
    13441 ccattatggc atccctgtac gaacccttcg ggctggtcgg tgtcgaatcc gccctatgcg
    13501 gcacacgcgt cgtcctctcc gaaaacatgg catgtacaga ggtcatgaac gaagaagccg
    13561 gattcttttt ctcacgacaa aacccggaaa ccctcgcgca agccgttgcc cgggccgtca
    13621 gccttaaaaa acagggcgga caccgcctgt ccgacccgat gcgggcgctg aactacaacc
    13681 cgtccctgtc gcaccacatc gaccgactga ccgacatgct ggcatccgta taacaaaatg
    13741 ccgtctgaac gggttttcag acggcatttt cgcaaccgtt gcagccgtgc ggcaaaaagc
    13801 taacgggcaa ttttcaaaac gtcgtacaga tagttgcggt aatttccgac caactcccgg
    13861 ttcgggcagt cttccaaccc tccgccgcac aaaccgcgca tcgccatatt cgcacgcatg
    13921 aagccgtctt tggcttccaa accagccgcc tccatcagta cgccgcctgc aaaggcaagg
    13981 tcgaggaaat cctgccgctg tacgaatccg ccggcaatat tgctcctgac ggcaaactgc
    14041 gttacataat ccggctgagc gtaatcccat ttcttcctga cggacacgcc ctcaaacggt
    14101 acctgatgat cgccgaaata accgaaaaca aagggtttgc cgcgttcgtg cagataacgg
    14161 tcgaaacttt ccaccgcttt gtccaaatcg gcaatgcgcc cgatgtagtc gttaagcgcg
    14221 gatacggttt tcgcattcaa atcgggcgca tccaaatcaa acacattgtc ggtatccgtc
    14281 cgatacggcc cgtgctcctt catggtcagc acatatacga acatcggctg ccgcacgttt
    14341 tccaaatcgg gatggcgttt ttcgagaatc atccgcgcat actgcatcat ttcctcactg
    14401 gaaatgtgcc acaggttttt gcccatcggc gcgggatagc ccaaatcttg cggctggaac
    14461 atcagattaa agccgaaatg gtcatatgct gccttggcat tgtagttgcc cttggtaaat
    14521 ggcgagagcg ccacgcaaaa ataaccgtgt tcgcgcaggt tgcggacaaa accggtctgc
    14581 aaatgcggta cgaccgaata aaacacgccg cttgccaacg cgccgaaatc tgtggacgga
    14641 acacccgcca aaaacgcaaa ttcggacttc cacgttgcgc cgccgaaagt atgcacgcgc
    14701 aaaggtgagg aaaatacagt atcttcctgc cgtccgaaca ttttcaaatc gggaattttg
    14761 gcggcggcaa aatcgaaaca gtgcggatcg agcgtcgatt ccatcagggt aacgacaata
    14821 tcgggctttt catccgacat gccgtacggc cgggtttcag acggcatctg cctggcaaac
    14881 gcttccccgt cgccctcgaa cacgggtact tggaaaaaca ccgcccgaca ggacatcggc
    14941 aggttcaaaa acacgtcgcg cccgtcgtcc ggcagcgagt ccagccacgt ctttaccgcg
    15001 ccggggtgct tggaaaaatg ccgcaccgac acgaacgccg ccgcaaacag aaccgcgccc
    15061 gcccaacgcc acggcatacc caaagaatcc gcaccgctcc agccgaaaac cgcatatgcc
    15121 gccaaagcca gcagccccgc cataccgata accgcttcct tataatgaaa cagcgtttcc
    15181 caattccgcc agtcggcaat cagcaaaaag tcggaaatca acagcggctg tttgtaataa
    15241 tggattttca gcctgtggaa cagtatcagc accacaaacc agaccgaagc gaaattcaag
    15301 gcgcgctgcc actgcgccgt caacatcagc ataccgcccg ccaaaaagac aaacagcgcc
    15361 gacgcgaaaa aatacgtcca gcggtagtgc gacctgacaa tcagcagcac cgccgccgtt
    15421 acaaacaaaa ataggaaagc ataggcaacc atcccgcccc actcctcaaa acggcatacc
    15481 tgtgccgttg cgaattatta tcgaaagcgt gcattataat gcagcacagg cgcatttcca
    15541 ccgatatttc agtataatgc catccccgaa cccgcccaac ccaaaggaac cgtgatgaaa
    15601 ctcatcattc tcgaccgcga cggcgtcatc aaccgggacc gcgacgactt cgtcaaatcc
    15661 gttgacgagt ggatacctgt cgaaggcagc atggatgcgg tggcattcct gacgcaggca
    15721 ggctgcaccg ttgccgtcgc caccaaccaa tccggcatcg gccgcaaata ttttaccgtt
    15781 caagacctca ccgaaatgca tgccaaaatg caccgcctca tccgtcaggc gggcggggaa
    15841 atcgacggca tctggttctg cccgcatacc ggcgccgacg gctgcaactg ccgcaagccc
    15901 aaaccgggca tgattgaaga catcctcgaa cgcttcaacg cccaagcttc ggaaacttgg
    15961 ctggtcggcg acagcctgcg cgatttgcag gcaatcgatg ccgtcggcgg aaaacccgcg
    16021 ctggttctga ccggaaaagg caaaaaaacg ctctcccaac acggacacga attacccgga
    16081 cacacacagg ttttcgatac cctgctcgat ttctcacaat acatcatgca ggaaaacgcc
    16141 gcaccgcaag ccgactgaac ataccgcatt tccgacaagg caaaaccatg ctcatcatcc
    16201 gcaacctgat ttactggctg atactctgtt ccagcctgat tttcctcttt ccctttatgc
    16261 tgctcgcctc gcctttccgg gacggggcgc acaagatggc gcgggtctgg gtcggcatcc
    16321 tcaactggtc gctcaaacac atcgtcgggc tcaaataccg catcatcggc gcggaacaca
    16381 ttccggaccg cccctccgtc atctgcgcca aacaccaaag cggctgggaa acgctcgcgc
    16441 tccaagagat ttttccgccg caggtttacg ttgccaagcg cgagttgttc aaaatcccct
    16501 ttttcggctg gggcttgaaa ctggtcaaaa ccataggcat agaccgcaac aaccgccgcg
    16561 aagccaacga acagctcata aaacagggtt tggcgcgcaa aaacgaaggt tattggatta
    16621 ccattttccc cgaaggcacg cgccttgcgc ccggaaaacg cggcaaatac aaactcggcg
    16681 gcgcgcgcat ggcgaaaatg tttgagatgg acatcgtccc cgtcgccctc aacagcggcg
    16741 aattttggcc gaaaaattcc tttctgaaat atccggggga aatcaccgtc atcatctgtc
    16801 cgaccatccc gcacgcaagc ggcagcgaag ccgaattgat ggaaaaatgc gaacacctca
    16861 tcgaaacgca gcagccgctc atttccggcg caggcccgtt tgccgccgaa atgccgtctg
    16921 aaaccgcatg acctccctga ttcacaccct ttcagacggc atagaactga ccgtcgaaat
    16981 caaccgccgt gccaagaaaa acctgattat ccgccctatc ggcacacata ccgtccgcat
    17041 cagcgtccca ccctgcttct ccgtctccgc cctcaaccgc tggctgtatg aaaacgaagc
    17101 cgtcctgcgg cggacactgg cgaaaacacc gccgcacaat acggcaaacc gactgcccga
    17161 acatatctgg ttccacggca ggcagcttgc ccttaccgcc catcaagaca cgcaaatcct
    17221 gctgatgccg tctgaaatcc tcgtccccga aggcgcgccc gaaaaacagc ttgcgctgct
    17281 gcgggacttt ttggaacggc aggcgcacag ttacctgatt ccccgtctcg aacaccacgc
    17341 ccgcgccacg caactgttcc ccgcctcctc ctcgctgacc tccgccaaaa ccttctgggg
    17401 cgtgtgccgc aaaaccacag gcatacgcct caaccggcgg ctggtcggcg cacccgaata
    17461 cgttgccgac tatgtctgca tacacgaact ctgccacctc gcccatcccg accacagccc
    17521 cgccttttgg gaactgaccc gccgcttcgc cccctacacg cccgaagcaa aacagcggat
    17581 caaaatccac ggcagggagc ttttcgcctt gggctgacgc agccccgggc cgacgctttc
    17641 agacggcatc cgtgccggaa caggcacgcg cccgcccgat tcaaaccgcg atgacgcttt
    17701 gccgccggtt cggggcagga tggcggcatc cgcgccgtcc gcaccgtttc atttcacacc
    17761 gcccttccga aacccgcccg atccgacgtg cggtatgaaa cgcttgagcc ggcgcgaagt
    17821 cttctactga tttgcccgcg aaaataccgt ccgaaaggtt ttcggacggc atttttttgc
    17881 gttttcctgg atggcggcgg attcgtaaaa ggcgggcagg gtggattgca ggatgggttg
    17941 aggcctgcca aatccgccaa ctctaccgaa tacgccgccg tcattcccgc gcaggcggga
    18001 atttggtttt gtcggtgcgg aaacttatcg gataaaacgg tttcttagat tccacg
//
LOCUS       DAXDXW010000048        17949 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXDXW010000048 DAXDXW010000000
VERSION     DAXDXW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17949
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.049"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(50..1471)
                     /locus_tag="ACFB64_001336"
     CDS             complement(50..1471)
                     /locus_tag="ACFB64_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC1209689.1"
                     /translation="MRHLPLFSLMLFPASVYAADSDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQDRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILITLTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINTMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGPLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLVFFVFKLL"
     gene            complement(1830..2189)
                     /locus_tag="ACFB64_001337"
     CDS             complement(1830..2189)
                     /locus_tag="ACFB64_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC1209690.1"
                     /translation="MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLMMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2186..2791)
                     /locus_tag="ACFB64_001338"
     CDS             complement(2186..2791)
                     /locus_tag="ACFB64_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 family protein"
                     /protein_id="HFC1209691.1"
                     /translation="MTIMNIDTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIVQIAPFHPYFQFEGTDSDGIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2838..3320)
                     /locus_tag="ACFB64_001339"
     CDS             complement(2838..3320)
                     /locus_tag="ACFB64_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC1209692.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3524..3621
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3832..4932
                     /gene="gcvT"
                     /locus_tag="ACFB64_001340"
     CDS             3832..4932
                     /gene="gcvT"
                     /locus_tag="ACFB64_001340"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC1209693.1"
                     /translation="MTALKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVVGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPDTEAVAFFKVLQTA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGRTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLEKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRSGQKQFD"
     gene            5079..5618
                     /locus_tag="ACFB64_001341"
     CDS             5079..5618
                     /locus_tag="ACFB64_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209694.1"
                     /translation="MFFDTEGKSHTAIFMKIVGGNGKIAGEVVIDGEYALDIVRMNDD
                     LSIAVQDNKGRSRASDLEALSQAKSFTLIAFSRYAGTVEVDKVSAVTGICADYRSKGL
                     KVAVADNMRPKQEIVVFRASGVMASNKRKINEAYLDYSASSKLPSAQKQELEQDKNNM
                     IAVHYGRLEELIARGICVR"
     gene            5670..6056
                     /gene="gcvH"
                     /locus_tag="ACFB64_001342"
     CDS             5670..6056
                     /gene="gcvH"
                     /locus_tag="ACFB64_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC1209695.1"
                     /translation="MSNNIPTELKYVASHEWLRLEEDGIITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQSGVVESVKAASDVYAPIAGEVVAVNDDLPGAPETANSDPYGAGW
                     FFKIKPANPADYDGLLTAEQYAGEVD"
     gene            6209..7456
                     /gene="hemA"
                     /locus_tag="ACFB64_001343"
     CDS             6209..7456
                     /gene="hemA"
                     /locus_tag="ACFB64_001343"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC1209696.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYTLDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRAAQEQESMGAKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKNPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            7703..10009
                     /locus_tag="ACFB64_001344"
                     /pseudo
     CDS             7703..10009
                     /locus_tag="ACFB64_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753899.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            10138..12105
                     /gene="nosZ"
                     /locus_tag="ACFB64_001345"
                     /pseudo
     CDS             10138..12105
                     /gene="nosZ"
                     /locus_tag="ACFB64_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
     gene            12390..12545
                     /locus_tag="ACFB64_001346"
                     /pseudo
     CDS             12390..12545
                     /locus_tag="ACFB64_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            12542..13633
                     /locus_tag="ACFB64_001347"
                     /pseudo
     CDS             12542..13633
                     /locus_tag="ACFB64_001347"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            13691..14587
                     /locus_tag="ACFB64_001348"
     CDS             13691..14587
                     /locus_tag="ACFB64_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC1209697.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVSQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQTAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGPDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIVVMKNGVKVADGSMD
                     ELHVQSGLPLTVNIRLNAPRTLSSRWQPLSDGISYRAQCQAEERMELLGELGSLSDLA
                     YFDIHTPTLDEMYARFLKREDV"
     gene            14584..15414
                     /locus_tag="ACFB64_001349"
     CDS             14584..15414
                     /locus_tag="ACFB64_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC1209698.1"
                     /translation="MNPVWIITGKEARDSLRNRWVLAAVLLLAALALSLGFLGSSPTG
                     SVKVDPLTVTVVSLSSLSIFLIPLIAMLLSYDALIGEIERGTMALLLSYPIWRNQILA
                     GKFVGHLIILALATTAGYGLAGITLQLANGGFDIAVWKPFALLIAASVILGAAFLSMG
                     YLISAKVKERGTAAGISIGVWLFFVVIFDMALLGILVADSKQVITAPVVETVLLFNPT
                     DIYRLLNLTGYENTAMYAGMAGLSGQIGLTVPVLLTAQVLWVIIPLVLAAGIFRKRRI
                     "
     gene            15411..15905
                     /locus_tag="ACFB64_001350"
     CDS             15411..15905
                     /locus_tag="ACFB64_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC1209699.1"
                     /translation="MKKTLLAIVAVFALSACRQAEEAPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTVKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDA
                     YIFK"
     gene            complement(16039..17700)
                     /locus_tag="ACFB64_001351"
     CDS             complement(16039..17700)
                     /locus_tag="ACFB64_001351"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC1209700.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VAEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GVGKDGGPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFIYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENFEEVESFDSGKEAGNYQKLFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAVCKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIIE
                     ENGSPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
BASE COUNT         4348 a         4637 c         4861 g         4103 t
ORIGIN      
        1 attatgccgg aatttaaaat gccgtctgaa tgttcagacg gcatagcgtt tacagtagtt
       61 tgaaaacgaa aaagacgagg gtatgtacga tgaagacggg tgtcaggaag gcgaccgacc
      121 acatcatata gccgaagaaa gtcggcatcg gtacgccgcg ctgttcggca atggccttga
      181 ccatgaagtt cggtgcgttg ccgatgtagg tcagtgcgcc catgaatacc gaacccatag
      241 aaaccgccag cagcgaatga aacaggggac ccgtcattaa ggcttgggca tcgccgcccg
      301 ccatattgaa aaacacgaga taagtgggcg cgttatccaa gaatgccgac aatatgccgc
      361 tcatccagaa atacatcgta ttaatcggat gacctgccgt atcgtgaacc agcgatacca
      421 ccccgcccag cgcgcctgcc tcgcctgctt tcagaatgct caggacgggg aagatggtga
      481 tgaagatgcc gaggaagagt ttgcccactt cggcgatggg ttcaaagttg aattcgttgc
      541 ctgcgcggac ttgtttgggc gtgattgcca tagatacggc ggtcaatgta atcaggatga
      601 catcgcggac aaggttttgc agggcgtaac ggctgccgag gatttcaaat cccgggtgtt
      661 cgggtttcca aaggccggac attagaaccg cgccgaccac gcccgaaagc aggaggaagt
      721 tccatttgcc gaagatggcg attttttcgg gtttttcctg ttgtgccggc gtatcttgtg
      781 caatgctttc ctgtttgaag aaacggttgt cgatgaaata gaaggcggtc aacaggacag
      841 cggtgctaat caggacgggg gcgaacatat gtttgaccgt ccacatgaaa tctacgcctt
      901 tgaggaagcc gaggaagagt ggggggtcgc ccaaaggggt caggccgccg ccgatgtttg
      961 caaccaggaa aatgaagaag atgacgatgt gcacgcggcg ggtacggtct tggttggctt
     1021 tcagcagcgg acgaatcatc agcattgccg cgccggtcgt tcccatgata gaggcaagtg
     1081 ccgtaccgac ggcaagcagg gcggtgttga gcttgggtgt gccgttcaag tcgccccaaa
     1141 ccaaaatgcc gcctgaaatg gtgtacaggg caagcagcag caggatgaag gggatatatt
     1201 cttcaacgag tgcgtgtgcg acggtatgga taccggcgga cgcgccaaaa accaaactga
     1261 acgggatgag gaagagcaat gtccaaaagg cggtgatttt gccgtaatgg tgatgccagg
     1321 tatgcgaaaa aaacaaggga cccaatgcga tagacagcaa aatcagggca aagggcaagc
     1381 cccacagcag gtttaggttt gcgccgtccg aatctgcggc gtaaaccgat gccgggaaaa
     1441 gcattagtga aaacaggggt aggtggcgca tcgtgtttcc tcgattcaag cactgccttg
     1501 cgcggcgcgc gggagtgata caggcttcgt gccgcccgga cataggcgga ctgtgctgcc
     1561 tgtttgtttt tgaaaaaagt tttccgattg attgtaaagt aaataatcag gataaaccag
     1621 tttcccaaac cggaaggcgg cgggaaggcg gattgccgtg cttgggaata tatgttcttt
     1681 ttgataaata attttattta aaacaaataa atatatgtct ttaataaata atctgtcgaa
     1741 aacgaaaaat aaatttattt taacatatat ttgcaatgaa acaggtttgc cccacccgtt
     1801 tgtttgccct tatccctttc agacggcatt caagattcgg gtctgcgcca catccatatg
     1861 gcgacaaggg aacaaaaaac cgatgaaacc gccccgaccc accagcgttg gggaaactgc
     1921 caaaacatca tcaggcagga tgcggtcatc atgctgatgg cgaaaatctt ggctttgcgc
     1981 ggcactgcgc cgttttgttc ccagttatga accatcgggc cgaaatagcg gtgccggtgc
     2041 agccagcggt gaaagcgcgg ggatgccttt gcccagcagg cggcggagag tagtacgaac
     2101 ggcgtggtcg gcaacagcgg caaaaaaatg ccgatgatcc ccagcagcag ggagatgccg
     2161 ccgcaggcaa ttaaaagata acggatcatt ttgaaatatt tttcttattg tgcggataag
     2221 ggcaggatgt gataccgagt tttgcccagc cttcatgtcc cattttttcc agcagggcga
     2281 tattgcgttc gaatattgcc gaagcgtcgg gaaaggcttg tgcggctttg gcaatgctgt
     2341 cttcgcggat gaggtgcagc gtcggatagg gagaacggtt ggtgtagttg ccgatgccgt
     2401 ctgaatccgt gccttcaaat tggaaatagg gatgaaacgg ggcgatttgg acgatgcctt
     2461 ccaagccgtt ttcgacaacg gcggcatcgg caatgtcgag catatcgttg aatacgtcga
     2521 aatcggggaa tagggtcggg tgaaccagca gggtggtttc cagttcggtg gcgggtgtat
     2581 tgcccagtcg ctgcagttct tcgtccaagt cttccaaaaa accgtcaagg tgtttggctt
     2641 cgctgatcgc gatgcggaca aggtttttaa cgtggggggc tttggcaaag ggacacaggt
     2701 tcagaccgat gacggctttt tccaaccatt gtccggtgtg ttcggcaaca gcatctttat
     2761 tttcggaagt atcgatattc attattgtca tgtaaatgtg tttgcagagt gcacgtgcgg
     2821 gaaaatcggg aagggcacta ttccttcagc aggtggttga gcggcaggga ggtggtgtgt
     2881 ttgatttctt ttaaaacaaa gctcgattgc gcatcctgta cgccgtggtg ggataaaagc
     2941 gtatccaaaa caaaatggga aaacgcgttc atatcggtaa aaaatgcctg aagcaggtag
     3001 tcggtctcgc cggttagggc gaagcagctc aagacttccg gccattttcg aaccgatgcg
     3061 gcaaaatctt cccgcgcgtc ttttgctttg cggatggaaa cgcggataaa tgcctgaaga
     3121 cctaagttga cggattccgg agacagcagc gcggcatatt ggcggacgat gccggcatct
     3181 tccaactgct tcagacggcg caggcacgga gaaggcgaaa gtgcgacacg ttcggacagt
     3241 tcgacattgg tcagcctgcc gttttcctgg aggacctgta agattttaat atcggttttg
     3301 tctaaagtga gttggggcat atttgcgttc cgttttaagg aattcggatt gtctgtccgt
     3361 atgtttgcgg caatccgcat agatggagac catattaaca tataaaaagt tataccgtca
     3421 tccgggacaa attttgtttt cggaaaatca tgtgaaaaca gaggcggtcg gtttgcatct
     3481 cttttaagac ggcttgccca aaccgccgat tcaagacata atcgggaaat gtgcaggaga
     3541 gtgttacacc caactacaat gtaaccaccg aaggcgcaga cacccttaaa tcgctcaggt
     3601 atcagggact gcacattgaa acaaacaatc tggagagcgg cgttagaata acgtccaccg
     3661 aaggggagaa ggccgtctga accaccattc agacaaccgc gcaaagcagt gagcagactg
     3721 gtttgccatc atgcggatac ggccgaaaat ctcaggttca aggacagata gggtcatccg
     3781 cgcacaggtg cgcgggcggc atctgaacaa aaaatccgga gaaacttgag aatgactgct
     3841 ctgaaaacca ccccgtttca tcaagcccat caagatgcag gcgcgaagcc ggtagatttt
     3901 gccggctggg aactgcccat ccattatggt tcgcaaatcg ccgaacacga agccgtgcgc
     3961 accgacgccg gtatgtttga cgtatcccat atgctcgtta ccgacgtcgt cggagcgaac
     4021 gccaaagcct ttttccgcaa attgattgcc aacgacgttg ccaaactcgc ttttgtcggc
     4081 aaagcccttt attccgcttt gctcaacgac aacggcggcg tgattgacga cttgatcgtt
     4141 taccgcacca acgaggccga aacccaatac cgcatcgtgt ccaacggtgc gacccgtgaa
     4201 aaagacacgg cacaattcca caaagtcgga caagagttcg gcgttgcctt caatccgcgc
     4261 tacgacctcg gtatgcttgc cgtacaaggt cctaaagcca ttgaaaaact cctgaccgtc
     4321 aaacccgaat gggcggatgt gattcacggc ctcaaaccgt tccaaggcgc ggatttgggc
     4381 aacgactggt ttgtcgcccg tacaggctac accggcgaag acggcgtcga agtgattctg
     4441 ccggataccg aagccgtcgc attcttcaaa gtcctgcaaa cagcaggcgt acagccttgc
     4501 ggcctcggcg cgcgcgacac cctgcgcatg gaagccggta tgaacctcta cggcaacgat
     4561 atggacgacg acaccagccc gctcgaagca ggcatgggcc ggacggttga tttgaaagac
     4621 gaaagccgcg attttgtcgg taaagccgcc ttgttggcat tgaaagaaaa aggcgttgcc
     4681 gtcaaacaag tcggtttgct gctcgaaaaa ggcggcatcc tgcgcgcgca tatggaagtg
     4741 ttgaccgaca aaggaaaagg cgaaaccacc agcggcgtat tctcgcccag cctgaaacaa
     4801 tctatcgcca tcgcccgcgt accgaaagat tttgacggcg ataccgccaa agtcctgatt
     4861 cgcggcaaag aagcggacgt gcgcgtactg aagctgccgt tcgtccgcag cggtcaaaaa
     4921 cagtttgatt aatatcggtt cagacggcat ttgtttgaat taagccgtct gaaagcaggt
     4981 tttaaggaat tgttatttcc tatgaaagca aattttggca gtttattgtt gttattgatg
     5041 ttatcggctt gcgcttcgtc gccggcgagc gcggattgat gtttttcgat acagaaggaa
     5101 aatcccatac tgcaatattt atgaaaatcg ttggcggcaa tggaaagatt gccggcgagg
     5161 tggtgataga cggggaatat gctcttgata ttgtccgcat gaatgatgat ttgagtattg
     5221 ccgtgcaaga taataagggt agaagccgtg cttccgacct ggaagcattg tcgcaagcga
     5281 aatcatttac cctaattgcg ttcagccgtt atgcgggcac agttgaagtg gataaagtat
     5341 cggctgttac aggcatttgt gcagattacc gcagcaaagg cctgaaggtt gccgttgcag
     5401 acaatatgag gcccaaacaa gaaatcgttg tattccgtgc aagcggcgta atggcaagta
     5461 ataagcgcaa gataaacgaa gcatatttgg attattctgc atcctctaaa ctgccttccg
     5521 cccaaaagca agaattggaa caagataaaa acaatatgat tgccgtccat tacggacgct
     5581 tggaagaatt aatcgcacga ggtatctgcg tacgctaagt tgtgcagatg ccgtctgaaa
     5641 ataaccccct accaatggag aatcaaacca tgagcaacaa catcccaaca gaactgaaat
     5701 acgttgccag ccatgaatgg ctgcgccttg aagaagacgg tatcattacc gtcggtatta
     5761 cccatcacgc gcaagagctg ttgggcgaca tcgtgtttgt cgagctgccc gaagtcggtg
     5821 cgaacctcgc cgccgaagaa cagtccggtg tggttgagtc tgtaaaagcc gcatccgacg
     5881 tgtatgcgcc gattgcgggc gaagtcgttg ccgtcaacga tgacttgcca ggcgcgccgg
     5941 aaaccgccaa cagcgaccct tacggcgcag gctggttctt caaaatcaaa cctgccaatc
     6001 ctgccgatta cgacggtctg ctgactgccg aacaatacgc gggcgaagtg gattaaaccg
     6061 cccgtttgtc cgacggcaac cgtcggacaa acggaaactg caccttcaga cggcatttct
     6121 gcggtcggag gtgcagtttt tttgtccgtg ttttaaggat gcagttaggc tataataacg
     6181 gtctatatcc atcttcaccg attttttcat gcaacttacc gctgtcggac tcaatcatca
     6241 aaccgcacct ttaagcatac gggaaaagct ggcgtttgcc gccgccgccc tgccagaagc
     6301 cgtccgcaat cttgcccgaa gcaatgcggc aacggaggcg gtaatccttt ctacctgcaa
     6361 ccgcaccgag ctttactgcg tcggcgattc ggaagaaatc atccgatggc ttgccgatta
     6421 ccacagtttg ccgattgaag aaatccgtcc gtatctgtac acgctggata tgcaggaaac
     6481 cgtgcgccac gccttccgcg ttgcctgcgg cttggattcg atggttttgg gcgagccgca
     6541 gattttgggg cagattaaag atgcggtgcg tgcggctcaa gaacaggaaa gtatgggggc
     6601 aaaactcaat gccctgttcc aaaaaacctt ttccgttgct aaagaagtcc gtaccgatac
     6661 cgctgtcggc gaaaattcgg tttcgatggc ttccgcgtcc gtcaagttgg cggaacagat
     6721 ttttcccgac atcggcgatt tgaacgtatt gtttatcggc gcaggcgaaa tgattgagct
     6781 ggttgccact tattttgccg ccaaaaatcc ccggctgatg acggttgcca accggacgct
     6841 ggcgcgtgca caggagttgt gcgacaagct cggtgttaac gccgaaccgt gcctgctgtc
     6901 cgatctgcct gccattctgc acgattacga cgtggtggtt tcttcaacgg cgagccagct
     6961 tccgatagtc ggcaaaggca tggtcgaacg cgcattgaaa cagcgtcaga gtatgccgtt
     7021 gttcatgctt gacttggccg tgccgcgcga tattgaagcg gaagtcggcg atttgaacga
     7081 tgcgtatctt tatacggtgg acgatatggt caacatcgtc caaagcggca aggaggcaag
     7141 gcagaaggcc gccgccgccg ccgaaacgct ggtgtccgag aaggttgccg aatttgtcag
     7201 gcagcagcag ggcaggcaga gcgttccgct gattaaggcc ttgcgggacg agggcgagaa
     7261 agcgcgcaag caggtgttgg aaaatgcgat gaaacagctt gccaaaggcg caacggcgga
     7321 agaggttttg gaacggctgt ccgtccaact gaccaacaag ctgctgcatt cgccaactca
     7381 aaccttgaat aaggcggggg aagaagataa agatttggtt catgccgtcg cgcagattta
     7441 tcatttggac aaataacggt gcgccggaaa aggatgccgt ctgaaggggt ttcagacggc
     7501 atatttttgt gccgcctgac aacatcgtga aatcccacat tatatcgatg taattacaaa
     7561 gtattatcct atatgcggat tggttttaag atttgtaaat ttgatttgca tcaaaaatcg
     7621 ccgatagatg attcatataa tatcaatatt aaagagtatc ggtatatcgg ggatagctat
     7681 gtcctgtttt tcaatccggc gtatgtctgc gtttcgggcg cggataacgg cgttttttac
     7741 cgcctttgtc tttttgacgg cggcactgcc cgcttatgcg gagcgtctgc ctgattttct
     7801 ggcgaaaata cagccttcgg aaatttttcc gggtgcggat cgttacggca agccggaagg
     7861 caagcctatg gttgcccgcg tttacaaagg cgatgagcag ctcggtttgg tttatatcac
     7921 gaccgatgcg gtcaatacgc gcggttattc gagcaaaccg atcgatacgc tgatggcttt
     7981 ggcaaacgac ggcacgatag ccggggcgaa actggtcgat catcacgaac cgattatgct
     8041 gatcggtatc ccgcaatcgc gtgtcgataa gttcatcgac aaatatatcg gtctgaattt
     8101 tattaaaaat ccgccgaccc cgagcgtggc gccgggcgac atcatcagcg gtgcgactgt
     8161 tacactgatg gtggttaacg acagcatcca gcgttcgtac aaggtcattg ccaaccaata
     8221 ccgtctgggt tcggacaagg cccttcagac ggcatccgct tccgatgttc gggaagccgc
     8281 gcctgcgtca gaaacccgtc cgcgccgtat ggcaaatccc gacaagcagg atattttgtc
     8341 ttgggacgaa cttttgaaac aaaaggccgt cggccatctg catatcacgc tcgatcaaat
     8401 caacaaactg tttgagaaag gcggcaaggc cggcgtggcc gatcacgccg aacagggcga
     8461 tcctgacgat acctttattg atttgtatgt tgccttggtc agccagcctt ccatcggtaa
     8521 aagcctgctg ggtaaggacg gctgggcgca tctgcaaaaa cggctgaaac ccgggcagca
     8581 ggcggttttg gttgccggag agggccgtta ttcttggaaa ggttcgggct atgtgcgcgg
     8641 cggtattttc gaccgtatcg agatgattca gggggagaac agcttccgtt ttaccgatgc
     8701 ccaacacgaa cgcgtcgtcg agctgtctgc cgccgatgcg ccgcgtttta aagaagtttc
     8761 ttggtttacc atccctgaag gcgtagcgtt tgacggtgcg gagccgtggc ggctgtaatt
     8821 gatggttcag cgcgtattga gtgtgaacga taaagccttc gttaccgccg atttggatta
     8881 cgaactgccc caagcttatt acgtcgatga tcctaaagcg ccgcctgtag aaatcagcgc
     8941 gcctgtcgaa gcagtacctg ccgcagcatc cgataccgct tcagacggca ttgccgaaga
     9001 tgcgtcagct gaaaacggtg tatccaacca attatggaaa cagatttgga aagcgaaaca
     9061 ggggcagatt gtcgtcgtcg gcattgcgct gaccatcctg cttttggtat tcctgttcca
     9121 agattggatt gtgcgttacg aaaaatggta cgaccgcttc cgctttgctt tcctaacttt
     9181 tacgctcttc tatatcggct ggtatgcaca ggcgcagctt tcggtcgtca atacgctgac
     9241 gctgttttcc gccatcctta ccgaatttca ttgggaattc ttcctgatgg atccgattgt
     9301 gttcatcctg tggctgttca cggctgcaac catgctgctg tggaacaggg ggacgttttg
     9361 cggttggttg tgtccgttcg gctcattaca ggaattgact aaccgcattg ccaaaaaact
     9421 gggtgtgaaa cagattaccg taccgcatat gctgcatacc cgtttgaacg tcataaaata
     9481 cctgatttta ttcggttttt tggcaatttc gctgtatgat ttgggtacgg cggaaaaatt
     9541 tgccgaagtc gaaccgttca aaactgcgat tatcttgaaa ttcatgtgcg attggtggtt
     9601 tgtcgccttt gccgtggcac ttttgattgc ggggctgttt atcgaacgct tcttctgccg
     9661 ttatctgtgt cctttgggtg cgggcatcgc gctgccgggc agattccgcg tgttcgactg
     9721 gctgcgccgc tacaaaatgt gcggcaaccc ctgccaaatc tgtacgcacg aatgccccgt
     9781 tcaggcaatc gcgcccgagg gcgacatcca tccgaacgaa tgtatccagt gcctgcactg
     9841 ccaagtgatg taccaccacg atacgcgctg tccgcaagtt gtggcggcaa ataagaaaaa
     9901 acaaaaacag gctgctgcca aatcagggga attggaaaat gtctctaagc agccgcagga
     9961 acaggtcgtc cgttttgtca aacccgaaac ggcgcaatct gaaaaataag catccggggg
    10021 aagcctgaag ccaaagtgtc aggcaggcag ggtttccccg gaaggataat aaaatttttt
    10081 tcccatcggc ttgccgtccg aaaaccggtt tgccgtattt tatccaagga gtgttgtatg
    10141 tcagacgaaa aattagaaca aaacggcttg agccgtcgtt cgttcttagg tacggccgcc
    10201 gcttccggtg caggtattgc cggtgcgggc ctgttgggtt tggcgggttg ctctaaagac
    10261 ggcgaacagg ctgccgctaa cgcttccggc gcggctcccg tcgccaaggc gcaaggggaa
    10321 tccaaacccg gccaactgtc ttccgaagtc ggtccgggcg aactcgatca atattacggt
    10381 ttcctttccg gcggccagtc cggcgagatg cgcctgatcg gtctgccttc tatgcgcgaa
    10441 ctgatgcgta tccccgtgtt caatatggac agcgcgaccg gttggggacg caccaatgag
    10501 agcttgaaag tcctcaacgg caatattacc gaagaaaccc gtaaattctt aaaagacggc
    10561 ggcctgcgct gctaccccaa cggcgacttg caccacccgc acctgtcgtt taccgaccaa
    10621 acttatgacg gccgctatgc ctatgccaac gacaaggcaa acaaccgcgt ctgccgcgtg
    10681 cgtttggatg tgatgaaggc cgacaaaatc atcgacatcc ctaatgattc aggtattcac
    10741 ggtctgcgtc cgcaacgtta tccgaaaacc ggttatgttt ttgccaacgg cgaacacatt
    10801 acccctgtca gcggtgtagg caaactggat gatgcgaaaa cctggaatgc agtgtacacc
    10861 gccatcgacg gcgaaaccat ggagatcgca tggcaagtat tggttgacgg taacctggac
    10921 aacggcgatg ccgactatca aggcaaatat tcttttgcca cctgctacaa ctccgagcgc
    10981 gcgctgaccg tacaaggtgc gtcttccaac gagcaggact ggtgcgtcgt tttcgacctg
    11041 aaagccatcg aagaaggcat caaagcgggc gacttcaaag aagttaacgg tgtgaaaatg
    11101 ctggacggcc gcgctgaggc caaatccaaa tacacgcgtt atatccctgt gccgaattct
    11161 cctcacggct gtaacgcaag ccctgacggt aaatacatca tgcccaacgg taaactgcct
    11221 ccaaccgtta ccgtattgga tgtcagcaaa ctggacgatt tgttcgccgg caaaatcaaa
    11281 gagcgcgatg tggtcgtagc cgaaccgcaa ctgggtctcg gcccgttaca cactgcattc
    11341 gacggtcgcg gcaatgctta tacgacattg tttatcgaca gccaaatggt gaaatggaat
    11401 attgacgatg cgatcaaagc ctacaaaggc gataaaatcg atccgatcaa acaaaaactc
    11461 gacgttcact atcaaccggg tcacaaccat acgaccatgg gcgaaaccaa agaagccgac
    11521 ggtcaatggc tggtgtcctt gaacaaattc tccaaagacc gcttcttgaa tgccggtcct
    11581 ttgaaacccg agtgcgacca attgatcgac atctccggcg acgaaatgcg tctggtacac
    11641 gacaacccga cttttgccga accgcacgac ttgtgtttgg ttgccgcgtc caaactgaac
    11701 ccgggcaaaa cttgggaccg caaagacccg tggttctagc aggatgcttt ggaacaggcg
    11761 aaaaaagacg gtgtcgagct ggaaaaagcc gccaaagccg tacgcgaagg caacaaagta
    11821 cgcgtgtata tgacggccgt cgcgcctgct tacagtatcc cgcagtttga agtgaaccaa
    11881 ggcgacgaag taaccgttta cgttaccaac gtcgaaacca tcgaggacct gacccacggc
    11941 ttcactttgg aaggctgcgg catcgctatg gaaatcggcc cgcaagccac ttcttccgta
    12001 accttcaaag ccgtccgccc gggtgtgcac tggtactact gccaatggtt ctgccacgca
    12061 ttgcatatgg aaatgtccgg tcagatgatt gttcatccca aataatgcgg cccgacggct
    12121 gaatatggag aatggcgtct gaaggcaaca ggtcttcaga cggcatttat agccaaaccg
    12181 cttgccgtac cgtttcaggc ggtttcgcta taaaaacagg cacatttgaa tgatgcttta
    12241 ttttgttcgg ccataccgaa cgaaacagag ggacactatg cacacatccg cgctccgtat
    12301 atggctgaag gccgtgctga tacttgccgc cggcagcatt tttcagacgg catcggcagc
    12361 cgtcgtccac gtttccccgc aagacaacct tgccgaaatt tttgcccgcg cccgggcggg
    12421 cgatacgatc aaactggctt ccggcgtata tcaaaccaaa ctttatatcg acaaaccgat
    12481 tacgattgaa gggcctgccg accgttccgc aaccatcgaa ggggacaaga gcgggcgtac
    12541 catagccgta cacgcgccgg acgtaacgct ccgcaacctg accgttaccc gttccggtat
    12601 gagcctgcca gcaatggatg ccggtattta tctcgaaaaa gccgccccgc gcgccctgat
    12661 tgaacacaac aatatttttg ataattcgtt cggcgtatac cttcatggtt ctgccgatgc
    12721 gatggtgcgc gagaataaaa tcgtcggcga tgcgaccttg cgcgtgaatg agcgcggcaa
    12781 tggcgttacc gtttggaacg cgcccggcgc gcaggtcgtc ggcaacgaca tttccaaagg
    12841 gcgggacggc attttttcca ataccagcac gcacaacacc tataaaaaca accgcttcag
    12901 cgacctgcgt ttcgccgtcc actatatgta caccaacgac agcgaagtca gcggcaatat
    12961 ttgcgtgggc aacaatatgg gctatgtgct gatgttttcc gaacggctca aagtgttcga
    13021 caatatcgcc gtcggcagcc gcgattaggg catcatgctc aactatgtca actattccga
    13081 tattcacgac aatattatca acaaagcggg caagtgcgtt tttgcctaca atgccaacta
    13141 cgataaactg tccgccaatc attttgaaaa ctgccaaatc ggcatgcact ttaccgccgc
    13201 catcgaaggc acgtccctgc acgacaattc ctttatcaac aacggaagcc aggtcaaata
    13261 tgtcagtacg cgctttctcg actggagcga gggcggacac ggcaactact ggagcgacaa
    13321 cagcccgttc gatttgaacg gcgacggctt cggagacagc gcgtaccgtc ccgacggcat
    13381 catcgaccaa atcatctggc gcgcgcccgt atcgcgcctc ttgatgaaca gtcccgcaat
    13441 cagcatcgtc aaatgggcgc aggcgcagtt tcccgccgtt ctgcccggcg gcgtggtgga
    13501 cagcaaaccg ctgatgaagc cttatgcccc caaaattcaa acccgttatc aggcgatgaa
    13561 ggacgagttg ctcaaagaag ccgaaacgcg gcagtcggaa cggggcaggg cggaaaacgg
    13621 ttctttgaac tagtctgctt cagacggcac ccggattcaa atgccgtctg aaaacacaaa
    13681 aggaacaacc atgaccacac atcatgtcga attgaggaag gtaaccaaac ggttcggggc
    13741 gcaaaaagcc gtcagccaag tcgatttggt tttgaaggca ggagaaagcg tcgggcttgc
    13801 cggacacaac ggcgcgggca agtccaccat catgaagctg atactcgggc tgattacccc
    13861 gaccgaaggc gaagtgatgc ttttgggcga acgtaccggc agcaaagcgg gggcgcggct
    13921 tcgcagccaa atcggctacc tgcccgaaac cgttgcgctg cacccttcgc tgaccggcat
    13981 cgaaacgctg gatttttatg ccaaacttaa aaaacagccg ctcacgcaga accgggggct
    14041 gcttgagcgc gtcggcattt cccagaccgc acaccgccgc gtcggcactt attctaaagg
    14101 gatgcgccaa cgccttgcct tggcacaagc cctgctgggc gagcccaaag tcctgctgtt
    14161 tgacgaaccg acaaccggcc ccgaccctgc atcacgacaa atgttttacg aagtcgtgcg
    14221 cgaactcaac gggcgcggcg cgaccgtatt gctcagtacc cacgcccttg ccgagttgga
    14281 cggacacgcc gaccgcattg tcgtgatgaa aaacggcgtt aaggttgccg acggcagtat
    14341 ggacgaattg cacgtccaaa gcgggctgcc gctgaccgtc aatatccgcc tcaatgcgcc
    14401 gcgcaccctc agcagccgct ggcagccgct ttcagacggc atttcctacc gggcgcaatg
    14461 tcaggccgaa gaacgtatgg aactgttggg cgaattgggc agcctgtccg accttgccta
    14521 tttcgacatc cacacgccga cgcttgacga aatgtacgca cggttcttga aaagggagga
    14581 cgtatgaacc ccgtttggat tattaccggc aaagaagccc gcgacagcct gcgtaaccgc
    14641 tgggtgcttg ccgccgtcct cctgcttgcc gcactcgccc tttctttggg tttcctcggc
    14701 agctcgccca ccggttcggt caaggtcgat ccgctgaccg ttaccgtcgt cagcctgtcg
    14761 agcctgtcta ttttcctgat tccgctgatt gcgatgctgc tttcttatga cgcactgatc
    14821 ggcgaaatcg aacgcggtac gatggcgttg ctgttgagtt atcccatttg gcgcaaccaa
    14881 atccttgccg gcaagtttgt cggacacctc atcatcctcg cccttgccac cacggcaggt
    14941 tacggattgg caggcattac gctgcaactt gccaacggcg gtttcgacat cgccgtttgg
    15001 aaaccctttg cgctgttgat tgccgccagc gtgattttag gtgcggcatt tctgtctatg
    15061 ggctacttga ttagtgcaaa ggtcaaagag cgggggacgg cggccggtat ttccatcggc
    15121 gtatggttgt ttttcgtcgt catcttcgat atggcgcttt tgggtattct ggttgccgac
    15181 tcgaaacagg tcatcaccgc gcccgtcgtt gaaacggtgc tgctgttcaa tcccaccgac
    15241 atttaccgcc tgctcaacct gaccggttac gaaaatacgg ctatgtatgc gggtatggcg
    15301 ggtttgagcg gacaaatcgg cctgaccgtc cccgttttgc tgaccgcgca ggttttatgg
    15361 gttatcattc cgcttgtttt ggcagccgga atttttagaa agcgacgaat atgaagaaaa
    15421 ccctgttggc aattgttgcc gttttcgcct taagtgcctg ccggcaggcg gaagaggcac
    15481 cgccgccttt accccggcag attagcgacc gttcggtcgg acactattgc agtatgaacc
    15541 tgaccgaaca caacggcccc aaagcccaga tttttttgaa cggcaaaccc gatcagcccg
    15601 tttggttctc caccgtcaag cagatgttcg gctataccaa gctgcccgaa gagcccaaag
    15661 gcatccgcgt gatttacgtt accgatatgg gcaatgttac cgattggacg aatcctaatg
    15721 ccgacacgga gtggatagat gcgaaaaaag ccttttacgt catcgacagc ggctttatcg
    15781 gcggtatggg cgcggaagac gcgctgccgt tcggcaacaa ggagcaggct gaaaaatttg
    15841 caaaggataa aggcggcaag gtcgtcggtt ttgacgatat gcccgatgct tacattttca
    15901 agtaaatcca aacagaaatg ccgtctgaac gttgaagcca aggttcagac ggcatttttt
    15961 gtcataggtt caaacggggc aggttcgaac aactgcccca ttccttattc ctgaccgggc
    16021 gtaacggcgg aatgaggttt acatcccgcc gtaattcggt ccgcttgcgc cttcggggca
    16081 aatccaagtg atgttttgcg tcgggtcttt gatgtcgcag gttttgcagt gcacgcagtt
    16141 tgccgcgttg atttgcaggc gcggactgcc gttttcttcg atgatttcat atacgccggc
    16201 cggacaatag cgcgtttcgg gagaggcgta ttctttatag ttcacgtcta tcatcgtttg
    16261 cggattgttc agcaccaaat ggtcgggctg gttttcttcg tgcgcgagat tggcaaggaa
    16321 gacgctgctc aagcggtcaa aggtcaacac accgtcgggt ttcggataat caatcggctt
    16381 gcacacggcg gcttttttga gctgctcgtt gtctttgccg tgatgtttca aggtccacgg
    16441 ggctttgcct ctgaaaatca tctggtcgat gccggtataa agcgagccga ggtaaacgcc
    16501 ccatttgaat gacggacgga cattgcgcgc ggcataaagc tcttgataca gccagctttg
    16561 ttcaaacagt ttttgatagt ttcccgcttc tttgccgctg tcgaagcttt ccacttcttc
    16621 aaagttttcc aacaagggga acacggcttc ggcggcgagc atggcggatt tcatcgcggt
    16681 atgaatgcct ttgatgcgcg gcatattgag gaaccccgcc gcatcgccga ttaaaacgcc
    16741 ccccttgaac gagagcttcg gcaggctttg caaaccgcct tcaatcagcg aacgcgcgcc
    16801 gtaagcaatg cggcggccgc cttcaaaggt tttgcggatt tcgggatggg ttttgaaacg
    16861 ttggaactct tcaaacggcg acagatacgg gttttgatag tccaaaccga ccacgaagcc
    16921 gacggcgact ttgttgtcgt cgaaatggta aataaacgca ccgccgtagg ttttgctgtc
    16981 cagcggccag cctgcgctat gcaccaccaa accgggctga tgctgttcag acggcacttc
    17041 ccaaacttct ttgatgccca agccgtaagt ttgcggctgg ctgttttggt cgagttggaa
    17101 acgttcgatg atttgcttgg aaagcgaacc tcggcaacct tcggcaaaca gggtttgccg
    17161 cgcccaaagc tccatgccgg gctggaaact gtcggtcggc ccgccgtctt tgcctacacc
    17221 catattgccg gtcgcaatac ctttgaccga gccgtcttcg tgatacagca cttcggcggc
    17281 ggcaaagccc ggatagattt ccacgcccat attttccgcc tgctccgcca accagcgcac
    17341 gacttcgcct aagctgacga tgtagttgcc gtgattgtcg aaattcgggg taataggcag
    17401 attgaacgct tttttctccg tcaggaacaa caccttgtcc tgagttaccg tgcgcgtcag
    17461 cggcgcgcct ttttctttcc agtcgggaat cagctcattc agcgcaatcg gatcgataac
    17521 cgcgcccgcc agcgaatgcg cccccgcctc cgaacccttc tccgccacgc aaacgctgat
    17581 ttcgcgcccg ttctgttcgg caagctgctt gagtttgatg gcggcggaca accccgacgg
    17641 gcctgcgccg acaatcacga catcgtattg catactgtcg cgggtgatgg attctgtcat
    17701 ggcggttcct gtgtatttat tattgaattg caaatccgtg attatacaac gggaacatat
    17761 agttaccaaa tacaacaaag gtcgtctgaa aaccatattt tcggttttca gacgaccttt
    17821 gtcgaaattt caataagcac gccaccattt tacctgtccg accgcaaact ccgtctgacg
    17881 tttcggactg cgtgtgaaaa acgccttatc cccaccggca tccctccctt tcggcacgac
    17941 cgccaaaat
//
LOCUS       DAXDXW010000049        17863 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXDXW010000049 DAXDXW010000000
VERSION     DAXDXW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17863
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.050"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            17..328
                     /gene="rpsJ"
                     /locus_tag="ACFB64_001352"
     CDS             17..328
                     /gene="rpsJ"
                     /locus_tag="ACFB64_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC1209701.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHMNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(670..783)
                     /locus_tag="ACFB64_001353"
                     /pseudo
     CDS             complement(670..783)
                     /locus_tag="ACFB64_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1114..1758
                     /gene="rplC"
                     /locus_tag="ACFB64_001354"
     CDS             1114..1758
                     /gene="rplC"
                     /locus_tag="ACFB64_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC1209702.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGVAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1758..2378
                     /gene="rplD"
                     /locus_tag="ACFB64_001355"
     CDS             1758..2378
                     /gene="rplD"
                     /locus_tag="ACFB64_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC1209703.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLARDERLFVIEALTAETPKTKVFAEQVKNLALEQVLFVTKRLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            2375..2689
                     /gene="rplW"
                     /locus_tag="ACFB64_001356"
     CDS             2375..2689
                     /gene="rplW"
                     /locus_tag="ACFB64_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC1209704.1"
                     /translation="MNQQRLTQVILAPVVSEKSNVLAEKCNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRILGRRSDVKKAYVSLADGQELDLEAAAAAAD
                     KE"
     gene            2695..3528
                     /gene="rplB"
                     /locus_tag="ACFB64_001357"
     CDS             2695..3528
                     /gene="rplB"
                     /locus_tag="ACFB64_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC1209705.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGISAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGVVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            3534..3812
                     /gene="rpsS"
                     /locus_tag="ACFB64_001358"
     CDS             3534..3812
                     /gene="rpsS"
                     /locus_tag="ACFB64_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC1209706.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAVRASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3821..4150
                     /gene="rplV"
                     /locus_tag="ACFB64_001359"
     CDS             3821..4150
                     /gene="rplV"
                     /locus_tag="ACFB64_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC1209707.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            4160..4852
                     /gene="rpsC"
                     /locus_tag="ACFB64_001360"
     CDS             4160..4852
                     /gene="rpsC"
                     /locus_tag="ACFB64_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC1209708.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVIIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPIHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4836..5252
                     /gene="rplP"
                     /locus_tag="ACFB64_001361"
     CDS             4836..5252
                     /gene="rplP"
                     /locus_tag="ACFB64_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC1209709.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            5252..5443
                     /gene="rpmC"
                     /locus_tag="ACFB64_001362"
     CDS             5252..5443
                     /gene="rpmC"
                     /locus_tag="ACFB64_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC1209710.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            5443..5706
                     /gene="rpsQ"
                     /locus_tag="ACFB64_001363"
     CDS             5443..5706
                     /gene="rpsQ"
                     /locus_tag="ACFB64_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC1209711.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHSLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5928..6296
                     /gene="rplN"
                     /locus_tag="ACFB64_001364"
     CDS             5928..6296
                     /gene="rplN"
                     /locus_tag="ACFB64_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC1209712.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAVPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6308..6631
                     /gene="rplX"
                     /locus_tag="ACFB64_001365"
     CDS             6308..6631
                     /gene="rplX"
                     /locus_tag="ACFB64_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC1209713.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGGKVVVEGVNVVKRHQ
                     KPNPMRGIKGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            6641..7180
                     /gene="rplE"
                     /locus_tag="ACFB64_001366"
     CDS             6641..7180
                     /gene="rplE"
                     /locus_tag="ACFB64_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC1209714.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLERIAGQRPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            7183..7488
                     /gene="rpsN"
                     /locus_tag="ACFB64_001367"
     CDS             7183..7488
                     /gene="rpsN"
                     /locus_tag="ACFB64_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC1209715.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            7504..7896
                     /gene="rpsH"
                     /locus_tag="ACFB64_001368"
     CDS             7504..7896
                     /gene="rpsH"
                     /locus_tag="ACFB64_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC1209716.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7910..8443
                     /gene="rplF"
                     /locus_tag="ACFB64_001369"
     CDS             7910..8443
                     /gene="rplF"
                     /locus_tag="ACFB64_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC1209717.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKKLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVASEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            8457..8810
                     /gene="rplR"
                     /locus_tag="ACFB64_001370"
     CDS             8457..8810
                     /gene="rplR"
                     /locus_tag="ACFB64_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC1209718.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8829..9347
                     /gene="rpsE"
                     /locus_tag="ACFB64_001371"
     CDS             8829..9347
                     /gene="rpsE"
                     /locus_tag="ACFB64_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC1209719.1"
                     /translation="MAKHEIEERGNGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            9340..9525
                     /gene="rpmD"
                     /locus_tag="ACFB64_001372"
     CDS             9340..9525
                     /gene="rpmD"
                     /locus_tag="ACFB64_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC1209720.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTS
                     ENRGMINKISYLLKVES"
     gene            9527..9961
                     /gene="rplO"
                     /locus_tag="ACFB64_001373"
     CDS             9527..9961
                     /gene="rplO"
                     /locus_tag="ACFB64_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC1209721.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTVSANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEDVGGKIEM"
     gene            9973..11283
                     /gene="secY"
                     /locus_tag="ACFB64_001374"
     CDS             9973..11283
                     /gene="secY"
                     /locus_tag="ACFB64_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC1209722.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALLIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            11288..11506
                     /gene="infA"
                     /locus_tag="ACFB64_001375"
     CDS             11288..11506
                     /gene="infA"
                     /locus_tag="ACFB64_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC1209723.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            11527..11640
                     /gene="rpmJ"
                     /locus_tag="ACFB64_001376"
     CDS             11527..11640
                     /gene="rpmJ"
                     /locus_tag="ACFB64_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC1209724.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDPRHKQRQG"
     gene            11706..12068
                     /gene="rpsM"
                     /locus_tag="ACFB64_001377"
     CDS             11706..12068
                     /gene="rpsM"
                     /locus_tag="ACFB64_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC1209725.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            12088..12483
                     /gene="rpsK"
                     /locus_tag="ACFB64_001378"
     CDS             12088..12483
                     /gene="rpsK"
                     /locus_tag="ACFB64_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC1209726.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            12503..13123
                     /gene="rpsD"
                     /locus_tag="ACFB64_001379"
     CDS             12503..13123
                     /gene="rpsD"
                     /locus_tag="ACFB64_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC1209727.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            13149..14135
                     /gene="rpoA"
                     /locus_tag="ACFB64_001380"
     CDS             13149..14135
                     /gene="rpoA"
                     /locus_tag="ACFB64_001380"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC1209728.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVGGVQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKSPPIDPVLLHPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            14159..14527
                     /gene="rplQ"
                     /locus_tag="ACFB64_001381"
     CDS             14159..14527
                     /gene="rplQ"
                     /locus_tag="ACFB64_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC1209729.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLASRRLAFDRTRDRDVVVKLFGDLGPRFTTRNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            14674..15387
                     /gene="minC"
                     /locus_tag="ACFB64_001382"
     CDS             14674..15387
                     /gene="minC"
                     /locus_tag="ACFB64_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC1209730.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAVAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVNQGAEL
                     IADGNMHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            15416..16231
                     /gene="minD"
                     /locus_tag="ACFB64_001383"
     CDS             15416..16231
                     /gene="minD"
                     /locus_tag="ACFB64_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC1209731.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSRKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILRIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENRKMRFLEAEKKSFFKRLFGG"
     gene            16235..16498
                     /gene="minE"
                     /locus_tag="ACFB64_001384"
     CDS             16235..16498
                     /gene="minE"
                     /locus_tag="ACFB64_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC1209732.1"
                     /translation="MSLIELLFGRKQKTATVARDRLQIIIAQERAQEGQTPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            16502..17422
                     /gene="oxyR"
                     /locus_tag="ACFB64_001385"
     CDS             16502..17422
                     /gene="oxyR"
                     /locus_tag="ACFB64_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC1209733.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLKEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIHD"
BASE COUNT         4948 a         3382 c         4407 g         5126 t
ORIGIN      
        1 tgaaggatat tgataaatgg caaaccaaaa aatccgtatc cgcctgaaag cttatgatta
       61 cgccctgatt gaccgttctg cacaagaaat cgttgaaact gcaaaacgta ccggtgctgt
      121 tgtaaaaggc ccgattcctt tgccgaccaa aatcgagcgt ttcaacattt tgcgttctcc
      181 gcacatgaac aaaacttccc gtgaacaatt ggaaatccgc acccatttgc gcctgatgga
      241 catcgtggat tggaccgata aaactaccga tgcgctgatg aagctggatt tgccggccgg
      301 tgttgatgta gaaatcaaag tccaataatt cggactgtcg aaaatcccca agcaatcaat
      361 gcttggggat tttttatgtt atgccgtctg aaaatatgcg gcggcaaaca gatttaaggc
      421 aaaacaacaa aatcaaaaaa cagtgcggtc ggtgaaatcg gcttattttg tgcctcaaga
      481 attttcagac ggcatttttt agtgaacaaa acaggatatt ttttatccat aactttccta
      541 aggcttgccc cccgaaaaaa ggtagaatcg ggcttggttt gggcagaagc atccatctga
      601 ttctaaggat ggatgatgtt ttattctcaa gtaaataaag gaaagatgat gagcctacta
      661 atgtctttaa agatatagtg gattaaattt aaaccggtac ggcgttgcct cgccttgccg
      721 tactatttgt actgtctgcg gcttcgtcgc tttgtcctga tttaaattta atccactata
      781 ttttaaacaa caaagaattg aatttggaag aacggcatga tttggagcgg gcggttaaga
      841 tcttgcagga cacgcacgtc atcccggttt gtcggagaaa gatgcatacg ccgtgatggt
      901 cggataccga cactttaaaa gcatccgacc ccatgccttc tgaagattca aaacgcttca
      961 gacggcatat tgaagatatt tctgatattt ctgttgatat ttctttgact tgtcggatat
     1021 aatgtcaagc ttggtacatt tttgccaagt ttaactttat ctgaaagaca ggccaatcgc
     1081 agcctgtccc tttactttaa aaggaaaata atcatgactt taggtctggt tggacgcaaa
     1141 gttggtatga cccgcgtgtt cgacgagcag ggtgtttctg ttccggtaac cgttttggat
     1201 atgtctgcca accgcgttac acaagtaaaa tccaaagata ctgacggcta taccgccgtt
     1261 caagttacct ttggtcagaa aaaagccaat cgtgtcaaca aagccgaagc cggacacttt
     1321 gcaaaagcag gtgttgaagc cggtcgcggt ttgattgagt ttgctttgac tgaagaaaaa
     1381 ctggctgaat tgaaagccgg tgacgaaatc accgtttcta tgtttgaagt cggtcaactg
     1441 gtcgatgtaa ccggtacctc taaaggtaaa ggtttctccg gcacgatcaa acgtcataac
     1501 ttcggtgccc aacgtacttc ccacggtaac tcccgttctc accgcgttcc aggctctatc
     1561 ggtgtggcgc aagacccggg tcgcgtgttc cccggtaagc gcatggccgg ccaatacggc
     1621 aacaccaaag caactgttca aaaattggaa gttgtccgtg ttgatgcaga acgccaactg
     1681 ctgttggtta agggtgctgt tccgggtgcg gtcaacagcg atgttgtagt tcgtcccagc
     1741 gtgaaagtag gtgcgtaatg gaattgaaag taattgacgc taaaggacaa gtttcaggca
     1801 gcctgtctgt ttctgatgct ttgttcgccc gcgaatacaa tgaagcgttg gttcaccagc
     1861 tggtaaatgc ctacttggca aacgcccgct ctggtaaccg tgctcaaaaa acccgtgccg
     1921 aagtaaaaca ctcaaccaaa aaaccatggc gtcaaaaagg taccggccgc gcccgttccg
     1981 gtatgacttc ttctccgctg tggcgtaaag gcggtcgcgc gttcccgaac aaacccgacg
     2041 aaaacttcac tcaaaaagta aaccgtaaaa tgtaccgtgc cggtatggcg actatcctgt
     2101 cccaattggc gcgtgacgag cgtttgtttg tgattgaggc gttgactgcc gaaactccca
     2161 aaaccaaagt ttttgccgaa caagtaaaaa atttggctct ggagcaagtg ctgtttgtaa
     2221 ccaaacggct cgacgagaat gtttacttgg cttcacgcaa cttgccaaac gtattggttt
     2281 tggaagctca acaagttgat ccttacagct tgctgcgtta taaaaaagta atcatcacta
     2341 aagatgcggt tgcacaatta gaggagcaat gggtatgaat caacaacgtt tgactcaagt
     2401 aatcttggcg cctgtcgttt ctgaaaaaag caacgtattg gctgaaaaat gcaaccaaat
     2461 gacgtttaaa gtcttggcaa atgcaaccaa acctgaaatc aaagctgctg tcgagctgct
     2521 gttcggtgtt caagttgctt ctgtaactac cgttaccact aaaggcaaaa ctaagcgttt
     2581 tggtcgtatt ttgggtcgcc gcagcgatgt taaaaaagct tatgtaagct tggctgacgg
     2641 tcaagagttg gatttggaag ccgctgctgc agctgcagat aaggaataaa caaaatggca
     2701 atcgttaaaa tgaagccgac ctctgcaggc cgtcgcggca tggttcgcgt ggtaacagaa
     2761 ggtttgcaca aaggtgcacc ttatgcaccc ttgcttgaaa agaaaaattc tactgccggc
     2821 cgtaacaata atggtcatat caccacccgt cacaaaggcg gcggtcataa acaccattac
     2881 cgtgttgtag actttaaacg taacaaagac ggcatttctg ctaaagtaga gcgtatcgaa
     2941 tacgatccta accgtactgc cttcatcgca ctgttgtgct atgcagacgg cgagcgtcgt
     3001 tacatcatcg ctcctcgcgg tattcaagcc ggtgtcgtat tggtttccgg tgctgaagct
     3061 gccatcaaag taggcaacac cctgccgatc cgcaacattc ccgttggtac gactatccac
     3121 tgtatcgaaa tgaaacccgg taaaggtgct caaatcgcac gttctgccgg tgcttctgcg
     3181 gtattgttgg ctaaagaagg tgcatacgct caagtccgtc tgcgctctgg cgaagttcgt
     3241 aaaatcaacg tagattgccg tgcgaccatc ggtgaagtcg gtaacgaaga gcaaagtctg
     3301 aaaaaaatcg gtaaagccgg tgctaaccgt tggcgcggta ttcgtccgac cgtacgcggt
     3361 gttgtcatga atcccgtcga tcacccgcat ggtggtggtg aaggccgtac cggcgaagcc
     3421 cgcgaaccgg ttagtccatg gggtactcct gctaaaggct accgcactcg taataacaaa
     3481 cgcacggata atatgattgt tcgtcgccgt tactcaaata aaggttaatt agtatggctc
     3541 gttcattgaa aaaaggccca tatgtagacc tgcatttgct gaaaaaagta gatgctgttc
     3601 gcgcaagcaa cgacaaacgc ccgattaaaa cctggtctcg tcgttctacc attctgcctg
     3661 attttatcgg tctgaccatt gccgtgcaca acggtcgcac ccatgtgcct gtgtttatca
     3721 gcgacaatat ggttggtcat aaattaggcg aattctcatt gacccgtacc tttaaaggcc
     3781 acctggccga taaaaaggct aaaaagaaat aaggtgaatc atgagagtaa atgcacaaca
     3841 taaaaatgcc cgtatctctg ctcaaaaggc tcgtttggta gctgatttga ttcgtggtaa
     3901 agacgttgcc caagctttga atattttggc tttcagccct aaaaaaggtg ccgagctgat
     3961 caaaaaagta ttggagtcag ctattgctaa tgctgagcat aataacggtg cggacattga
     4021 tgaactgaaa gtggtaacta tctttgttga caaaggtcca agcttgaaac gttttcaagc
     4081 tcgcgccaaa ggtcgcggta accgcatcga aaaacaaact tgtcatatca atgtgacagt
     4141 gggtaactaa ggaaaagcta tgggacaaaa gattaaccct acaggctttc gcctggcggt
     4201 aactaaagac tgggcttcaa aatggtttgc taaaagtacc gacttttcta ctgttttgaa
     4261 gcaggatatc gatgttcgca attatttgcg tcaaaaattg gccaatgctt cggttggtcg
     4321 agtaattatt gaacgccctg caaaatctgc acgcattacc attcactccg ctcgtccggg
     4381 tgtggttatc ggtaaaaaag gtgaggatat cgaagttttg aaacgtgact tgcaagtctt
     4441 gatgggtgta cctattcatg taaatattga agagattcgc cgtcctgagt tggatgctca
     4501 aattattgct gacggtatcg cccagcagtt ggaaaaacgc gttcaattcc gtcgtgctat
     4561 gaaacgagca atgcaaaatg caatgcgttc tggtgctaaa ggcattaaga ttatgacttc
     4621 aggccgtctg aatggtgcgg atattgcccg tagcgaatgg tatcgtgaag gtcgcgtgcc
     4681 actgcatact ttacgtgcaa atgtagatta tgcaaccagc gaagcgcaca ccacatatgg
     4741 tgtattgggt ctgaaagttt gggtttatac ggaaggcaat attaaatctt ccaaacctga
     4801 acatgagagt aaacaaagaa aggcaggtag acgtaatgct gcagccaact agactgaaat
     4861 accgtaagca acaaaagggt cgcaataccg gcatcgctac tcgcggtaat aaggtaagtt
     4921 tcggtgagtt cggcttgaaa gccgtaggtc gtggtcgttt gactgcccgt caaatcgaag
     4981 ctgctcgtcg tgcaatgacc cgtcacatca aacgtggtgg tcgtatttgg attcgtgtat
     5041 tccctgacaa accgattact gaaaagccta ttcaagttcg tatgggtggc ggtaaaggta
     5101 acgtggaata ttacattgcc gaaattaaac caggtaaagt gttgtatgaa atggatggcg
     5161 ttccagagga actggctcgt gaagcattcg agttggctgc tgccaaattg cctattccta
     5221 caacctttgt agtaagacag gtgggtcaat aatgaaagca aatgaattga aagacaaatc
     5281 cgttgagcag ttgaatgcag atttgttgga cttgttgaaa gctcagtttg gcttacgtat
     5341 gcaaaacgct accggtcaat taggcaaacc aagtgaattg aaacgtgtac gtcgcgatat
     5401 tgctcgtatt aaaaccgttt taactgaaaa aggtgctaag taatgagcga aactaaaaat
     5461 gttcgtactt tgcaaggcaa agtagtaagc gacaaaatgg ataaaaccgt aacagtattg
     5521 gttgagcgta aagtaaaaca ttcgctgtat ggtaagatta ttcgattatc tactaaaatc
     5581 catgcccatg atgaaaataa tcaatatgga attggtgatg tggttgttat atcggaatcc
     5641 cgtccattgt caaaaactaa atcttgggtt gtcagtgagc tggttgagaa agcacgttct
     5701 atttaagaat taaagcaacg tgcttggaat gggaaacgaa gtattgcagc aaatttaatt
     5761 tgcgtgtaaa cttcgtttcc tgtctttcag tttcttctgg aagtttcttc cctttcggga
     5821 tccaagactg gtttacttga accgcaaggt ttcatttaat aagcagcggc tttgctgtaa
     5881 gttatctgaa agtggtaaat taagttggtt aatttaaagg taataatatg attcaaatgc
     5941 agaccatctt agatgtggct gataactctg gtgcgcgtcg cgtaatgtgt atcaaggtat
     6001 tgggcggatc taagcgtcgc tacgcttctg ttggcgatat tattaaagtg gcagttaaag
     6061 atgcggttcc gcgtggccgt gtcaaaaaag gcgatgtata taatgcggta gttgttcgta
     6121 ctgctaaggg tgtgcgtcgt cctgatggtg cgttaattaa attcgataac aatgccgccg
     6181 tgttgctgaa taataaactt gaacctttgg gtactcgtat ctttggtccg gtaacccgtg
     6241 aattgcgtac tgagcgattt atgaaaatcg tttcattggc acctgaagta ttataaggaa
     6301 tagcacgatg aataaaatca ttaaaggcga tagggttgta gtaattgctg gtaaggataa
     6361 aggtaagcag ggtcaagtag ttcgagtgtt gggtggtaaa gttgttgttg agggcgttaa
     6421 tgttgtaaaa cgccatcaaa aacctaatcc aatgcgtggc attaagggcg gtattattac
     6481 taaagaaatg cctttggata tttctaatat cgcaatcctg aatccggaaa ctaataaagc
     6541 ggaccgtgtt ggtattaagc tgattgaaaa tgaaggcaaa gttaaacgcg ttcgtttctt
     6601 caaatcaaat ggctctatca ttggagcata aggagataac atggctcggt tgagagagtt
     6661 ttataaagag acagttgttc ctgaattggt taaacaattt ggttacaaat cagtaatgga
     6721 agtcccgcgt attgaaaaaa tcaccttgaa tatgggtgtg ggtgaggctg ttgctgacaa
     6781 aaaagttatg gaacatgctg tttccgattt agagagaatt gccggtcaaa gaccggttgt
     6841 tactgttgcc cgtaaatcta tcgcaggttt taaaatccgt gataactatc cggttggttg
     6901 caaagtaaca ttgcgtcgtg atcaaatgtt tgaattcttg gatcgtttga ttactattgc
     6961 attacctcgc gtacgtgact tccgtggtgt gagcggtaaa tcatttgatg gccgtggcaa
     7021 ttacaatatg ggtgttcgtg agcaaattat tttcccggaa attgaatacg ataaaattga
     7081 tgctttgcgt ggtttgaata ttactattac tactacagca aaaaccgatg aggaagcgaa
     7141 agctttattg tcattgttta aatttccgtt caaaggataa tcatggctaa gaaagcactt
     7201 attaatcgtg atctgaaacg tcaagctttg gctaaaaaat atgcggctaa acgcgcggca
     7261 attaaagcgg taatcaatga ttcgaatgca actgaggaag agcgttttga ggctcgtttg
     7321 aggtttcaat ccattcctcg taatgcggca cctgtgcgtc aacgtcgtcg ttgtgctttg
     7381 acaggtcgcc ctcgtggtac tttccgtaaa tttggtttgg gtcgtattaa aatccgtgaa
     7441 atcgccatgc gtggcgaaat tccgggtgtt gttaaagcca gctggtaata ggagtaatta
     7501 agaatgagta tgcatgatcc tatttccgat atgttgactc gtatccgcaa tgcgcaacgt
     7561 gctaataaag cagcagttgc aatgccttct tcaaaattaa agtgtgctat tgcaaaggta
     7621 ttgaaagaag aaggatatat tgaggacttc gcagtttcat ctgacgtaaa gtctatattg
     7681 gaaattcaat taaaatacta tgcaggtcgt cctgtaattg aacaaatcaa gcgtgtatct
     7741 cgccccggtt tgcgtattta taaagcgtct agtgagattc caagtgttat gaatggcttg
     7801 ggtattgcta tcgttagtac ttctaaaggt gtaatgactg atcgtaaagc acgttctcaa
     7861 ggtgttggtg gtgagttgtt atgcattgta gcctagtgga ggaaaagaaa tgtcacgtgt
     7921 cgcaaaaaac ccagtgacgg ttcccgctgg tgtagaagta aaatttggaa cagaggcatt
     7981 ggttattaag ggtaagaacg gtgaattgtc ttttcctttg cattctgatg tagccattga
     8041 atttaatgat ggcaaattga cttttgttgc gaataatagc agtaaacaag caaatgcaat
     8101 gtctggtact gctcgcgcat tagtcagcaa tatggttaaa ggtgtttcag aaggttttga
     8161 gaagaaatta caattgatag gcgtgggtta tcgtgctcaa gcacaaggta aaatcttgaa
     8221 tctgtctttg ggtttttctc atccgatcgt atatgaaatg cctgaaggtg tctccgttca
     8281 aactcctagc caaacagaga ttgttttaac tggctcggat aaacaagttg ttggtcaagt
     8341 tgcttctgag attcgtgcgt tccgtgctcc tgagccttat aaaggtaaag gtgttcgcta
     8401 tgtaggcgaa gtagtggtaa tgaaagaagc caagaaaaaa taattgaggt tcactaatgg
     8461 ataaacatac aacccgactc cgtcgtgcac gcaaaacccg tgctcgtatt gcggacttga
     8521 aaatggtaag attatgtgtg ttccgaagca ataatcatat ttatgctcaa gtaattagtg
     8581 ctgaaggtga taaagtattg gctcaagcct ctacattgga agctgaggtg cgcggtagtc
     8641 tgaaatctgg aagcaatgtt gaagcagctg caatagttgg taaacgtatt gctgagaagg
     8701 ctaaagcagc aggtgtagaa aaggttgctt ttgatcgttc aggtttccaa tatcacggtc
     8761 gtgtgaaggc tttggctgaa gctgctcgtg aaaatggttt aagcttctaa atatttggag
     8821 actttcagat ggcaaaacat gaaattgaag aacgcggtaa cggtctgatt gaaaagatgg
     8881 tcgcagttaa ccgtgtaacc aaagtagtta aaggtggtcg cattatggct ttctctgcgc
     8941 taactgttgt tggtgatgga gatggtcgta ttggtatggg taaaggtaag tcaaaagaag
     9001 tgcctgttgc agtccaaaag gcgatggatc aagcacgacg ctctatgatt aaggtaccat
     9061 taaaaaatgg tacgatccat catgaggtta ttggtcgaca tggtgctact aaagtattta
     9121 tgcagcctgc taaagagggt agtggcgtaa aagccggtgg acctatgcgt ttggtttttg
     9181 atgctatggg cattcataat atttccgcca aagtgcacgg atctactaac ccatataata
     9241 tcgtacgtgc aacattagat ggtttgtcta agttgcatac tcctgctgat atcgcagcca
     9301 aacgtggctt gacagtggaa gacattttgg gagttaacca tggctgaaca aaaaaagatt
     9361 agggttacat tggttaaaag cctgattggt acaattgaat ctcatcgtgc atgtgcacgc
     9421 ggtttaggtt tgcgtcgtcg cgagcatacg gtagaggttt tagatacctc tgaaaaccgt
     9481 ggtatgatta ataaaatcag ctacttgttg aaagtggagt cttgatatgt ttttgaatac
     9541 aattcaacct gctgttggtg ctacgcatgc tggtcgtcgt gttggacgcg gtattggtag
     9601 tggtcttggc aaaacgggtg gtcgtggtca taaaggccaa aagagccggt ctggtgggtt
     9661 tcataaggtg ggtttcgagg gtggtcaaat gcccttgcaa cgacgcctcc ctaaaagagg
     9721 ttttaaatct ttaacagtat cagctaatgc acagcttcgt ttaagtgaac tggaatcaat
     9781 tgctgttaat gagattgata ttttagtctt aaagcaagca ggtctgattg catctacagt
     9841 ctctaatgtt aaagttattg cttctggtga aatttctaag gcagttgctt tgaagggtat
     9901 taaagttacc aaaggtgcga gagctgctat cgaggatgtt ggtggtaaga ttgaaatgta
     9961 aggtttaata ttgtggctaa tcaacaaacg tcatcaggtt catccaagtt tggagatctt
    10021 aagaaacgtc ttttgtttct atttggagca ttgattgttt ttcgaattgg tgcccatata
    10081 cccgtacctg gggttgatgc tgttgcttta gctaaattat acgaaagcgc tggaaacggc
    10141 atcctgggaa tattgaatat gttttccggt gggtcgttag agcgctttag tatatttgca
    10201 ataggaatta tgccatatat ttcagcttct attattgtac agctcgcttc tgaaattttg
    10261 ccatcattga aggctttaaa aaaagaaggg gaggctggta gaaaggtaat tacgaaatat
    10321 actaggtatg gtactgtttt gttagcaatt cttcaaagtc taggtgttgc atctttcgta
    10381 tttcagcaag gaattgttgt aacaagttca tttgagtttc atgtttccac ggtagtttct
    10441 ttggtaacgg gaaccatgtt tcttatgtgg cttggggagc aaattactga aaggggtatc
    10501 gggaacggta tttctttaat cattacggca ggtattgctt caggtattcc ttcgggtatt
    10561 gcaaagctgg ttacactgac gaaccaaggt tctatgagca tgcttacggc gttgcttatt
    10621 gtatttggtg ccttattatt aatttatttg gttgtatact ttgaaagtgc acagcggaag
    10681 attcctattc attatgcaaa acgccagttt aatggtaggg cgggtagtca aaatacgcat
    10741 atgcctttca agttgaatat ggctggtgtt attcccccaa tttttgcttc cagtattatt
    10801 ctatttccat ctactctttt aggttggttt ggttcggctg atacaaatag tgttttgcac
    10861 aaaatagctg gattgttaca acacggtcaa ttgctgtata tggctttatt tgcagcgaca
    10921 gttattttct tttgttattt ttatacggct ttggttttta gtcctaaaga aatggcagag
    10981 aatttaaaaa agagtggtgc ttttgttcct gggattagac ctggtgagca gacctctagg
    11041 tatttagaaa aagttgtatt acgtttgaca ttgtttggag ctctttatat tacaactatt
    11101 tgtttaattc cagagttctt aactacggtt ttaaatgtac ctttttattt gggtggcacg
    11161 tctttgttga ttctagttgt tgtaacgatg gattttagta cacaaataaa ttcgtatagg
    11221 cttactcaac agtatgataa gttaatgact cgttcagaaa tgaaatcatt ttctcggaaa
    11281 tagaattatg gcgaaagaag atactatcca aatgcaaggt gaaattcttg aaactttacc
    11341 taacgcaaca tttaaagtaa aacttgagaa tgaccatatt gtattgggtc atatttctgg
    11401 gaagatgcgg atgcattaca ttcgtatttc tccgggagat aaggtcacag tagagctgac
    11461 accttatgat ttaactaggg ctcgaatcgt tttcagagca agataaacca ataaaaggaa
    11521 aataaaatgc gtgtacaacc atctgttaag aaaatttgcc gaaattgcaa gattattcgt
    11581 cgaaatcgtg tagttcgtgt gatttgtact gatccccgtc acaaacagcg tcaaggttaa
    11641 tggaatattt cttttaatgt gattctgtga tatagtgaca aaatttgctc taaaaaggaa
    11701 aaaacatggc tcgtattgca ggggtaaata tccctaataa cgcacacatc gtaattggtc
    11761 ttcaggctat ttacggtatt ggtgctactc gtgctaaatt gatttgtgag gctgcaaata
    11821 ttgcgcctga tactaaagca aaagatttgg acgagactca attagatgct ttgcgtgatc
    11881 aagttgccaa gtatgaagta gaaggtgatt tgcgtcgtga agtaactatg agtatcaaac
    11941 gattgatgga catgggctgc tatcgtggct tccgtcatcg tcgcggctta ccatgccgcg
    12001 gtcaacgcac tcgtacaaat gcgcgtactc gcaaaggtcc gcgtaaagcg attgctggta
    12061 agaaataaat tttaaggaat tttattaatg gctaaagcaa acacagcttc acgtgtacgt
    12121 aaaaaagtac gtaaaaccgt gagtgagggt attgtgcacg ttcatgcatc tttcaacaat
    12181 accatcatta caatcactga ccgtcaaggc aatgcgttgt cttgggctac ctctggcggc
    12241 gctggtttta aaggttctcg taaaagtaca ccatttgcag cacaagttgc agcagaagca
    12301 gctggtaaag ttgcccaaga gtatggcgtt aaaaatttag aggttcgtat taaaggtcca
    12361 ggtccaggtc gcgaatcctc tgtacgtgct ttgaatgctc ttggtttcaa gattaccagc
    12421 attactgacg ttaccccgtt gcctcataac ggttgccgtc cgcctaaaaa acgtcgtatt
    12481 taatattgga gtgatttgaa acatggcacg ttatattggc cctaaatgta agttggcacg
    12541 tcgcgaaggt acggatttgt ttttgaagag tgcgcgccgc tctttggatt ctaaatgtaa
    12601 aattgattcc gctcccggtc agcatggtgc aaaaaaaccg cgtttgtcag actatggttt
    12661 gcagttgcgt gaaaaacaaa aaatccgccg tatttatggc gtattagaac gtcagttccg
    12721 tcgttatttc gcagaagctg atcgccgtaa aggttctact ggcgagttgc tgttgcagtt
    12781 gctggaatct cgtttggata atgtcgttta tcgtatgggt ttcggttcta cccgagctga
    12841 agcaagacag cttgtttctc ataaggctat agttgtaaat ggacaagttg tcaatattcc
    12901 ttctttccaa gtgaaagctg gtgatgttgt ctcagttcgt gaaaaagcca aaaaacaggt
    12961 acgtattcaa gaagcattgg gtttggcaac tcaaatcggc ttgccgggtt gggtttctgt
    13021 agatgcagat aaacttgagg gtgtgttcaa aaacatgccg gatcgctctg aattgaccgg
    13081 tgatattaat gaacagctgg tggtagagtt ctactctaaa taatgctagc tcagtgaggg
    13141 acagttaaat gcagaatagc acaaccgaat ttttgaaacc tcgtcaaatt gatgtaaata
    13201 ctttttctgc aactcgtgca aaagtatcta tgcagccatt tgaacgtggt ttcggtcata
    13261 ccttaggtaa tgctttgcgc cgtatcttac tgtcatccat gaatggtttt gctcctactg
    13321 aagtagctat tgccggtgta ttgcacgaat attctactgt tggtggtgtt caggaagatg
    13381 ttgttgacat tttgctgaat attaaaggta ttgtgtttaa actccatggt cgtagccaag
    13441 ttcaacttgt gttgaagaaa tcaggttcag gtgtcgtatc tgccggtgat attgagttgc
    13501 cgcatgatgt agaaattctg aatcctggtc atgtcatttg tcatttggct gataacggtc
    13561 aaattgaaat ggaaattaaa gtagagcaag gtcgtggtta tcaatctgtt tcaggtcgtc
    13621 aggtagttcg tgatgagaac cgtcagattg gtgcaatcca gttggatgcg agcttttcgc
    13681 ccatcagccg tgttagcttt gaggttgaac ctgcacgtgt agagcagcgg acggatcttg
    13741 ataaattggt tttggatatc gaaaccgacg gttctattga tcctgaggaa gctgtacgca
    13801 gtgcggcacg tattttgatt gatcagatgt ctatttttgc tgatttgcag ggtactcctg
    13861 tggaggaggt tgaagaaaaa tcacctccta tcgaccctgt tcttttgcat ccggtggatg
    13921 atctggaatt gacagtacgt tcagctaatt gtttgaaagc tgaggatatt tattatattg
    13981 gcgatttgat tcaacgcact gaaaccgagc ttcttaaaac gccgaatttg ggacgtaaat
    14041 ctttgaatga gattaaggaa gtattggcat ctaaaggttt gacactgggt tctaagttgg
    14101 aagcatggcc acctgtaggc ttggaaaagc cttaatgaag aattaaagga taattgatat
    14161 gcgtcatcgt aatggcaatc gcaaattaaa ccgtaccagc agtcatcgtg ctgcaatgct
    14221 gcgtaatatg gcgaattcat tattgactca cgaaactatt gtaacaactc tgcctaaggc
    14281 caaggaattg cgccgtgtag tagagccgtt gattacattg ggtaaaaagc cgtcattggc
    14341 aagccgccgt ttggcatttg accgtactcg cgaccgtgat gttgtagtaa aactgtttgg
    14401 cgatttgggt cctcgtttta ctactcgtaa cggtggttat gttcgagtgt tgaaatacgg
    14461 attccgtaaa ggtgataatg cacctctggc actggttgaa ttggttgaca aaccggctgc
    14521 tgagtaattt tagtcatata acgccatctg ccgaaaagca ggtggcgtta tttttgcaat
    14581 atctgataga taatatggta ttggctatta tgtttaaaat attaattgaa tagctaaggt
    14641 ttgcgcggta aacttacatc attaaaaaat tctatgatgg tttatataat gaatgccttc
    14701 gatataaagt cgacaaagat ggacgtattg tctatatctt tgcatacatc agacttgttt
    14761 gatttggaag atgtgctggt caaattgggc aagaagtttc aagagtctgg tgttgttcca
    14821 tttgttctgg atgttcaaga atttgattat cccgagtctt tggatcttgc tgcattggtt
    14881 tcgttgtttt caaggcatgg tatgcaaatt ttgggtctga agcattctaa tgaacgttgg
    14941 gctgctgtgg ctatgaagta tcatttgctg ttttgtctgt ctcattcgga aaatgttaaa
    15001 gaactgggtc aggttgaggt gcagaaaacg gaggatggtc agaaagcaag gaaaacagta
    15061 ttgattacat cccctgtccg taccggtcag caggtttatg ccgaagatgg cgatttgatt
    15121 gttacggggg cggtcaacca gggggcggaa ttgattgcag atggcaatat gcatatttat
    15181 gcgccgatga gagggcgtgc tttggccggc gccaaaggcg atacttctgc ccgcatattt
    15241 atccactcca tgcaggcgga gttggtttct gtcgcgggga tttaccgtaa ttttgaacag
    15301 gatttgccgg accatctgca caagcagccg gtacagatat tgttgcagga taaccgattg
    15361 gttatcagtg caattggctc agagtaattg tttgatattt taaaaaggaa atattgtggc
    15421 aaaaattatt gtagtaactt caggcaaggg cggtgtcggt aaaacgacta ccagtgcaag
    15481 tattgcaaca ggtttggcat tacgcggata taaaactgcg gtaattgatt ttgatgtggg
    15541 tttgcgcaac ctcgacctca ttatgggttg cgagcgtcgt gtcgtttatg acctgatcaa
    15601 tgtcattcag ggtgaggcga cgctcaacca ggctttgatt aaagataaaa attgtgaaaa
    15661 cctgtttatt ttgccggctt cccagactcg ggataaagac gctttgacac gcgagggcgt
    15721 agaaaaagtg atgcaggagc tgtccggcaa gaaaatgggc tttgagtata ttatttgcga
    15781 ctctcctgct ggtattgagc agggtgcatt gatggcgttg tattttgctg atgaagccat
    15841 tgtaacgacc aatcctgagg tttccagtgt gcgtgactcc gacaggattt tgggcatttt
    15901 gcaaagcaaa tcccgtaagg cggagcaagg cggttcggtt aaagaacatc tgttaattac
    15961 gcgttattct cccgaacgtg tggcaaaagg cgaaatgctg tctgtacagg atatttgcga
    16021 tattctgcgt attcctttgc tgggtgtgat tcccgaatcc caaaacgtct tgcaggcatc
    16081 caattccgga gaaccggtca tccatcagga cagcgtagcg gcttccgagg catataagga
    16141 cgttattgcc cgtcttttgg gcgagaaccg taaaatgcgt ttcttggaag ctgagaaaaa
    16201 aagcttcttc aaacgtctgt tcggaggata aggtatgtca ttgatcgaac ttttattcgg
    16261 tagaaagcag aaaacggcaa ccgttgcccg cgaccgcctt caaatcatca ttgcccaaga
    16321 gcgcgcccaa gaaggtcaga ctccggatta cctgccgact ttacgtaaag agttgatgga
    16381 agtcctgtcc aaatatgtga atgtttcatt agacaatatc cgtatttccc aagaaaagca
    16441 ggatggtatg gatgtgcttg agttgaacat tactttgccg gaacagaaaa aggtatagga
    16501 catgacctta accgaattgc ggtacatcgt cgcagtcgcc caagaacgtc atttcggccg
    16561 ggcggcgcgg cgttgttttg tcagccagcc cactttgtct attgccatta agaaattgga
    16621 agaagagctt gccgtctctt tgtttgaccg gagcagcaac gatattatta cgaccgaggc
    16681 gggggaacgt atcgttgcac aggcgcgtaa ggtattgaaa gaggcggagc ttatcaggca
    16741 tttggcaaat gaagaacaaa acgagctgga gggtgcgttc aaactcgggc tgatttttac
    16801 ggttgcgcca tacctgctgc cgaaactgat tgtctcgttg cgccgtactg caccgaaaat
    16861 gcctttgatg ttggaagaga attacacgca tactttgacc gagtcgctca aacgcgggga
    16921 cgttgacgcg attatcgttg ccgaaccgtt tcaagagccg ggcattgtta ccgaaccctt
    16981 gtatgacgaa ccgtttttcg tgattgtccc gaaagggcat tcatttgagg aactggatgc
    17041 cgtttcgccc cggatgctgg gtgaggagca ggttttgctg ctgacggaag gcaactgtat
    17101 gcgggatcag gtactctcaa gctgttccga attggcggcg aaacagcgca tacaggggct
    17161 gaccaataca ttgcagggca gctcgatcaa tacaatccgc catatggtcg ccagcggttt
    17221 ggcaatcagc gtgttgccgg caaccgcgct gaccgagaac gatcatatgc tgttcagcat
    17281 tattccgttt gaaggtacgc cgccaagccg gcgggtcgta ttggcgtatc gccgtaattt
    17341 tgtccgtccg aaggcgttgt cggcgatgaa ggcggcgatt atgcagtcgc agcttcacgg
    17401 ggtaagtttt atccacgact aggcgcagaa aatgccgtct gaaacgggtt tcagacggca
    17461 tttgttatct ttgccgtcaa tcaggcaaac ctatccgacc tacagttgct tcgaatcgtc
    17521 attcccgcgc aggcgggaat ctaggtctgt cggtgcggaa acttatcgga taaaacgctt
    17581 gctccaatct cgcgttccag attcccactt tcgtgggaat gacgggatca ggtttccgta
    17641 tggatagatt cgtcattccc gcgcaggcgg gaatctaggt ctgtcggtgc ggaaacttat
    17701 caggtaaaac ggtttctccg gtcccgagtc ctagattccc actttcgtgg gaatgacggc
    17761 atattttgac attgaataaa aaagactaaa aacagaaaac ggaaaacgga aaacggaaaa
    17821 cggaaaacgg aaaacggaaa acggaaaacg gaaaacggaa aac
//
LOCUS       DAXDXW010000050        17405 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXDXW010000050 DAXDXW010000000
VERSION     DAXDXW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17405
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.051"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            38..532
                     /locus_tag="ACFB64_001386"
     CDS             38..532
                     /locus_tag="ACFB64_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209734.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEAENKIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKDLFAEEVPENNIVCDISHFLHFADRA
                     EADIPNQSRLYAEYWWEMETFKMSVLKDWERSGRPLDWEKWQRQYQSEAFELVKNLNR
                     SKQE"
     gene            652..1785
                     /locus_tag="ACFB64_001387"
     CDS             652..1785
                     /locus_tag="ACFB64_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209735.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEVPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRGLSH"
     gene            complement(2288..2563)
                     /locus_tag="ACFB64_001388"
     CDS             complement(2288..2563)
                     /locus_tag="ACFB64_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC1209736.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAIHTAGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRQLWEQGEKMPSET"
     gene            complement(2560..3843)
                     /locus_tag="ACFB64_001389"
     CDS             complement(2560..3843)
                     /locus_tag="ACFB64_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC1209737.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFASGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAHEPVLPPSVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAITVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWMG
                     YVPFFRLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(3992..5410)
                     /gene="glnA"
                     /locus_tag="ACFB64_001390"
     CDS             complement(3992..5410)
                     /gene="glnA"
                     /locus_tag="ACFB64_001390"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC1209738.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGPAFDGSSIGGWKGIEASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEGLGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            5721..6530
                     /gene="aroE"
                     /locus_tag="ACFB64_001391"
     CDS             5721..6530
                     /gene="aroE"
                     /locus_tag="ACFB64_001391"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC1209739.1"
                     /translation="MHALPRYAVFGNPAAHSKSPQIHQQFALQEGVDIEYGRICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSDRALAAGAVNTLVWLEDGRIRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSPKIFRDCRLAYDMVYGEA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFKPDIRPVIEHMKAL"
     gene            6533..7234
                     /gene="mtgA"
                     /locus_tag="ACFB64_001392"
     CDS             6533..7234
                     /gene="mtgA"
                     /locus_tag="ACFB64_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC1209740.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISTNLKKALIASEDVRFAGHGGFDWGGIQNAIRRNR
                     NSGEVKAGGSTISQQLAKNLFLNESRNYLRKGEEAAITAMMEAVTDKNRIFELYLNSI
                     EWHYGVFGAEAASRYFYKKPAADLTKQQAAKLTALVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(7508..7583)
                     /locus_tag="ACFB64_001393"
     tRNA            complement(7508..7583)
                     /locus_tag="ACFB64_001393"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7548..7550),aa:Phe,seq:gaa)
     gene            complement(7774..8508)
                     /gene="lptB"
                     /locus_tag="ACFB64_001394"
     CDS             complement(7774..8508)
                     /gene="lptB"
                     /locus_tag="ACFB64_001394"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC1209741.1"
                     /translation="MSANNSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTGFYMIVGLIAADAGSVMLDGRELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRNPAPSLSGGERRRVEIARVLAMKPR
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRAVYLGENFKY"
     gene            complement(8579..9109)
                     /gene="lptA"
                     /locus_tag="ACFB64_001395"
     CDS             complement(8579..9109)
                     /gene="lptA"
                     /locus_tag="ACFB64_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC1209742.1"
                     /translation="MIQKICKLFVLIVIFATSPAFALQSDSRRPIQIEADQGSLDQAN
                     QSTTFSGNVIIRQGTLNISASRVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVTYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTINGSTKSGAKSASKT
                     GRVSVVIQPSSTQKTE"
     gene            complement(9090..9671)
                     /gene="lptC"
                     /locus_tag="ACFB64_001396"
     CDS             complement(9090..9671)
                     /gene="lptC"
                     /locus_tag="ACFB64_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC1209743.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPENSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGRRQAGKVETEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYNHKTGMLNFSSKVKAAIYDTKDM"
     gene            complement(9668..10204)
                     /locus_tag="ACFB64_001397"
     CDS             complement(9668..10204)
                     /locus_tag="ACFB64_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC1209744.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDDGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQA
                     GLEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIIRAKGTLGAALNGYIK"
     gene            complement(10270..11244)
                     /locus_tag="ACFB64_001398"
     CDS             complement(10270..11244)
                     /locus_tag="ACFB64_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC1209745.1"
                     /translation="MAENEKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVAAISNSG
                     ESDEITAIIPALKRKDITLVCITARPDSTMARHADIHITASVSQEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALIHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKGAIVSMSEKGLGMLAVTDGQGCLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLTGALNMHDLLAARIV"
     gene            11341..12396
                     /gene="tal"
                     /locus_tag="ACFB64_001399"
     CDS             11341..12396
                     /gene="tal"
                     /locus_tag="ACFB64_001399"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC1209746.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKRQNLSPKQRYETMAVADVRAACDVCLAEHESTGGKTG
                     FVSLEVSPELAKDAQGTVEEARRLHAAIARKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIQVVASFFISRVDSALDATLPDRLK
                     GKTAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGVHTVNTVPDATLKAFIDHGTAKATLTESADEARARLAEIAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            12408..12542
                     /locus_tag="ACFB64_001400"
     CDS             12408..12542
                     /locus_tag="ACFB64_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209747.1"
                     /translation="MPSERLSDGIALLTGHSAAYQGSALKILMGKKLMHDITRQHKRK
                     "
     gene            12547..12837
                     /locus_tag="ACFB64_001401"
     CDS             12547..12837
                     /locus_tag="ACFB64_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209748.1"
                     /translation="MAKALEIISPDEIYSDLIFKDPVPPHTIYTELMKLVGRNAGNER
                     IADRAFDFFSPALMDDSATEEQYNALYDLTLLEEPGMELDKDEITALINSLK"
     gene            12854..13741
                     /gene="gluQRS"
                     /locus_tag="ACFB64_001402"
     CDS             12854..13741
                     /gene="gluQRS"
                     /locus_tag="ACFB64_001402"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC1209749.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVTYQSRRYALYEETLYRLKTAGLVYPCHCNRK
                     DWQAGARRGTDGFVYNGRCRHPGQRPALQGKQPSWRIRVPDRDIGFSDGIVGSYTQNL
                     ARDIGDFVLFRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDSNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAITAP"
     gene            complement(13811..14827)
                     /gene="dusA"
                     /locus_tag="ACFB64_001403"
     CDS             complement(13811..14827)
                     /gene="dusA"
                     /locus_tag="ACFB64_001403"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC1209750.1"
                     /translation="MINKELIEHKGKRRLCVAPMLDWTDRHYRYLARQITRNAWLYSE
                     MVNAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGC
                     PSPRVQKGAFGACLMNEVGLVADCLNAMHDAVGIPVTVKHRIGVDRQTEYQTVADFVG
                     TLRDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGIT
                     TNEAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDNRAPIEYADLVQRLYTYSQV
                     QIQAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGGLIFEAWKEVERA
                     NTRE"
     gene            14947..16101
                     /locus_tag="ACFB64_001404"
     CDS             14947..16101
                     /locus_tag="ACFB64_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC1209751.1"
                     /translation="MATITKRRNPSGETVYRVQVRVGKKGYPAFNESRTFSKKALAVE
                     WGKKREAEIEAGPELLFKRGKVKMMTLSEAMRKYLNETLGAGRSKKMGLRFLMEFPIG
                     GIGIDKLKRSDFAEHVMQRRRGIPELDIAPIAASTALQELQYIRSVLKHAFYVWGLEI
                     GWQELDFAANGLKRSNMVAKSAIRDRLPTTEELQTLTTYFLRQWQSRKSSIPMHLIMW
                     LAIYTSRRQDEICRLLFDDWHKNDCTRPVRDLKNPNGSTGNNKEFDILPMALPVIDEL
                     PEESVRKRMLANKGIADSLVPCNGKSVSAAWTRACKVLRIKDLRFHDLRHEAATRMAE
                     DGFTIPQMQRVTLHDGWNSLQRYVSVRKRSTRLDFKEAMMQAQSDIKSGK"
     gene            complement(16151..16417)
                     /locus_tag="ACFB64_001405"
     CDS             complement(16151..16417)
                     /locus_tag="ACFB64_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFC1209752.1"
                     /translation="MNISKEQGLLIAYGGRPYIPPREVHKDFFAHIGFNAFKASGVRC
                     ELPFPIFRLSDSRKSEYFVSVRELAKAIADKEEKAKSEYRKFRS"
     gene            complement(16525..>17405)
                     /locus_tag="ACFB64_001406"
     CDS             complement(16525..>17405)
                     /locus_tag="ACFB64_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC1209753.1"
                     /translation="LRRLVAAKVRRYCGEHPGVFDGAAGSGQLEQYIEPSDFRAVEIQ
                     AEACKALLQNYPAAKVYNTSLFLYTDGEPQDCTVMNPPFSIKLKDLSEDEKSRIAQEY
                     PWKKSGVADEIFVLKGLENARRFGFFILFPGIAYRKSEQRFREIIGNRLAELNRIQNA
                     FEDTPIEVLLLVIDKDKTDGGCIRELYDCKTDTLLAADTWQIEPDLWQTVQEPAPPKE
                     KEDPVLLEHECRDAAAKRIARELRFSKMVNEIEGWPHAEFDGFCDRLCNLIQAEKYGK
                     KHYFPCSLPLFGGAAG"
BASE COUNT         4023 a         4626 c         4692 g         4064 t
ORIGIN      
        1 aatttaatcc actataattt ggaagaggca gcaacctatg gaaaatagat ttgaaaaaat
       61 cgttaaggat atttttagtt ttacccaatt tgtttccgaa gcggaaaata aaattcctaa
      121 ccaatcctcc cgttattacg actgctggtg ggaattggaa atcctgaatg cctccgcatt
      181 ggaagattgg gaaatatcgg gaaagcctga aaaatgggag aaatggacag aaaaatatca
      241 gaccgaagca ttacagtcag ttaaggatct atttgccgaa gaagttcctg aaaataatat
      301 agtttgcgat atttctcatt ttttgcattt tgccgatcgg gcagaagccg acattcccaa
      361 tcaatcacgt ttatatgccg aatattggtg ggaaatggaa acgttcaaaa tgtcggtttt
      421 aaaagattgg gagcgttcag gcagaccact agattgggaa aaatggcaaa ggcaatatca
      481 atccgaagca tttgaattag tcaaaaattt gaaccgaagc aaacaagagt aacaagccgc
      541 agcctgcata ggggttttta aagtacggca gcaaccgtaa attaaattaa aaatcaaaca
      601 atcaaagccc gtctgatata atcgatatat cctgttaaac gaaaaataaa tatgtttaaa
      661 aaattcaaac cggtactgtt gtcatttttt gcacttgtat ttgccttttg gctgggaaca
      721 ggtattgcct atgagattaa tccgcgttgg tttttgagcg atacggcaac tgaagtacct
      781 gaaaatccga atgcttttgt ggcgaaactt gcccgcctgt tccgaaatgc cgacagggcg
      841 gttgtcatcg tgaaggaatc gatgaggacg gaggaaagcc ttgccggagc tgtggatgac
      901 ggtccgttgc agtcggagaa ggattatctc gcgctcgcta tccggctcag tcgtttgaaa
      961 gaaaaggcga aatggtttca cgtaacggag caggaacatg gggaagaggt ttggctggat
     1021 tactatatcg gcgagggcgg tttggttgcg gtttcgcttt tgcaacgatc gccggaagcg
     1081 tttgttaatg ccgaatatct gtatcggaac gatcgtccgt tttctgtaaa tgtgtacggc
     1141 ggaacggctc acggggaaaa ttatgaaacg acaggagaat atcgggttgt ttggcaaccg
     1201 gacggttcgg tatttgatgc gtcggggcgc gggaaaatcg gggaagatgt ttatgagcat
     1261 tgcctcgggt gttatcagat ggcccaggta tatttggcga aataccggga tgtcgcgaat
     1321 gatgagcaga aggtttggga cttccgcgaa gagagcaacc ggattgcatc ggactcgcgc
     1381 gattctgtgt tttatcagaa tatgcgggaa ttgatgcccc gggggatgaa ggcgaacagt
     1441 cttgtggtcg gctatgatgc ggacggtctg ccgcaaaaag tctattggag tttcgacaat
     1501 ggaaaaaaac gccagagttt cgaatattat ttgaaaaacg gaaatctttt tattgcacaa
     1561 tcttcgacgg tagcattgaa agcggatggc gtaacggcgg atatgcagac ctatcatgcg
     1621 caacagacgt ggtatttgga tggcgggcgg attatccgcg aagagaaaca gggagacaga
     1681 ctgcctgatt ttcctttgaa cttggaagat ttggaaaaag aggtgagccg ttatgcagag
     1741 gctgcggcga gacgttcggg cggcaggcgc ggcctttctc actgaacgca aataccgtct
     1801 gaaaccggtt ttcagacggc attggagagt caaacgcttt ttgcaatgcc gtatatgccg
     1861 aacccggttc gctacaaatt gaaaaccaca atccaaaaaa cggaaataca cattatcaaa
     1921 caaacaaata agttgacata ttgtgcgata ttgagggctg taccgtattt tcccgttctt
     1981 tatatgagcg gcaggcatcc tcataaaaga agggataccg ataaggaaaa atccctcggt
     2041 ataaaaaata tactgattaa tcaatacgaa tccaacaaac agttcatcat tttcttttct
     2101 gttggcgtat aaaaaccgct cggaaaaaaa taaaatatca aaacggcaaa ataaaaatac
     2161 tgatatatgc cttcactttc atagggcgac gctctatgtg ccgtcctgtg tgttgaaacg
     2221 tcggttttcc ttaccgcctg ccccgctttt ccctgattgc tgccaactgc cgtccgaacc
     2281 tgaaccgtca ggtttcagac ggcatttttt ctccctgttc ccacaattgc cgtttttgtg
     2341 cggcggtcag ttttttgacg gcgatttcct gtttgagcgc agtgccttta tccatgccgc
     2401 ctgcaacgat acgcatcgcc accggtttga atacgcgggt atatttcgcg ccttttccgg
     2461 ctgtgtggat ggcaagccgc tgttgcggat tcgggctgat accgcaatag aacgcgctgt
     2521 tttcacacaa tatcaggtaa acgctccagt ttgacgcgtt catctgcctg catcctgggt
     2581 tttttccccg ttccaaggcg caactttcag cagcagcagc atacccggca gggcaagtat
     2641 gaaacacagc cggaaaaacg gtacataacc catccattcg atcagatagc ctgcaaagga
     2701 attgatgacc gtgcgcggca cggcggacag gctggtaaac agcgcaagct gcgttgccgt
     2761 aaacgcggga ttggtctcgc gcgccatata cgatacaaac gccgccgtcc ccaaccccac
     2821 gccgaccgct tccgcgccga taactgccgc cagcatcagc ctctcgcctg tgccgaccgt
     2881 gtcgaaatgt ccgaaccctg ccagccatac aaaccccaaa accgttattg cctgcaccgc
     2941 gccgaatagc cacaaggctt tgtttacgcc gattttcagc atccacacgc cgcccaagat
     3001 acctgccgcc actgccggcc acagtcctgc atttttggca atcaaaccga tgtcggtctt
     3061 gctgaaaccc atatccagat aaaacggcgt tgccaacgcg gttgccatac tgtcgccgag
     3121 tttgtaaagg aagataaaca gcagcacgca caccgccgaa gcgatgccct tgcgcgtaaa
     3181 aaattcttta aacggctcta ccacggtctg cttcaacgtt ttaggaacgg aaggcggcaa
     3241 cacgggttcg tgcgcgagaa acagcgtcat cagaaggccg ggcagcataa ataatgaagt
     3301 gataacaaat acttctgacc acggcatcct gtctgccaac accaaactca atgaaccggg
     3361 aatcagggcg gcaacccggt aggcgttcac atgaaccgag ttgcccaaac ccaattcttc
     3421 gtccgacaaa atctcgcgcc tgaacgcatc caatacaatg tcctgactgg cggaaaaaaa
     3481 agcgacaagc accgacaggc cggcaatcaa cggcagatga ttccgggggt ttaaaaaggc
     3541 atatgccgcc aaagccgcca gtaaccctgc ctgcgtcagc agcatccacc cgcgccgccg
     3601 tcccaaaacg ggcagcctga ccgcgtccat cagcggcgac cacaaaaatt tccaagtaaa
     3661 cggcagaccg atcaacgcca tcagcccgat gctcttcaaa tccacctgct cgctgcgcaa
     3721 ccacgccgga atcaggttaa tcagaaagta cagcggcagc cccgaggcaa accccgtaaa
     3781 aatacagacg agcatattac gggaaaagat ttgcccgata aaacctgatt tcgatgcagt
     3841 catcgttcgt acagccaaaa aacaaaagcc tattgtagca aatatcgccc gcctgctgaa
     3901 aaatgccgcc acgcgccgcc ggtttgccgt tccaatgcaa aatgccgtct gaaactgtgt
     3961 tcagacggca ttgctactga aacccgacgg tttacaggct gtaatacatt tcaaattcca
     4021 gcgggtgcgg cgccatacgg atgcggcgga catcttcctc tttaaaggcg atgtagctgt
     4081 cgatccagtc tttgctgaac acgccgccgc gcagcaggaa ttcgtggtcg gccttgaggg
     4141 cggcaagtgc ttcttccaaa gaagcgcaga cggtcgggac gagcgcgtct tcttccggcg
     4201 gcaggtcgta caggttttta tcggcagggt cgcccggatg gattttgttt tgaatgccgt
     4261 ccaaaccggc catcagcagg gcggcaaatg ccaaatacgg gttggcggtc gggtcgggga
     4321 aacgcgcttc gatgcggcgc gccttgctgc tgttcacaga cgggatacgg atggaagcgg
     4381 aacggttttt ggcggaatag gccaatttgg tcggtgcttc aaagtgcggc acgaggcgtt
     4441 tgtaggagtt ggtggacgga ttggtaatcg cgttcagggc tttggcgtgt ttgatgatgc
     4501 cgccgatgta gtagagcgcg gtatcggaca aaccggcata gccgtcgcct gcgaacaggt
     4561 tttgaccgtc tttccaaatg gattggtgga cgtgcatacc gctgccgttg tcgcccataa
     4621 tcggtttggg cataaaggtg gcggttttgc cgaaattgtg ggcaacgttt tggatgacgt
     4681 atttcatatc ttgggtttgg tcggcgcgtt tgaccaaagt ggcgaaacgg gtgccgattt
     4741 ccatttggct gccggtaccg acttcgctgt ggtggacttc gacttcgatg ccgagtcctt
     4801 ccaaaatgtt caccatggcg gagcgcaaat cttgaccgca gtcaatcggc gcgacgggcg
     4861 cgtagccgcc tttgacggcg gggcggtggc cggtgttttg accgtccata tgcaggccgc
     4921 ttgcccacgc gccgctttcg gacgtgattt cgtaacgggt tttgtgcatg tcggtttcaa
     4981 attctacgcc gtcgaagacg aagaattcgg gttcggggcc gaaataggcg gtgtcgccga
     5041 taccggaaga tttcaaatag gcttcggcgc ggcgtgcgat ggagcgcggg tcgcggtcgt
     5101 aaccctgacc gtcggcaggg tcgatgacgt cgcaggtaat gacgacggta acatcatcat
     5161 aaaaaggatc gacgaaggct gtggacgcat cgggacgcag ctgcatatcg gaagcctcaa
     5221 tgcctttcca gccgccgatg gacgagccgt caaacgccgg tccgttttca aaccactctt
     5281 cggggtcttc caacacgatg cgcgcaggca cggtaaagtg gtgctgcttg cctttggtat
     5341 cggtgaagcg caaatcgaca aagcgggctt cgctttcttc aatcaatttt acggcgtttt
     5401 taatggacat cttcagctcc tgaaaaaggg aaatggcaaa atatgcggaa cgcggcggct
     5461 tgacgcaaag tcggcacgat tttaccacgc cgatgccgtc tgaaaccggt tttgcgtcaa
     5521 attccaagcc ggaaaatata gtttcaaacg gcaagattgt caacaatatg cttcggtttg
     5581 tctgttcaaa agcggaatgc ccgccctttt gtccgaatgc cgacaaaaca gatgccgtct
     5641 gaaggcggtt tcagacggca tcgttcccat cttcgcgccg ccgttataaa atgaccgccc
     5701 cgcctttatg gaatatgact atgcacgccc tcccccgcta cgccgttttc ggcaaccccg
     5761 ccgcccacag caaatcgccg cagattcatc agcaatttgc ccttcaggaa ggcgttgaca
     5821 tcgaatacgg acgcatttgc gccgacatcg gcggtttcgc gcaggcggtt tcgacatttt
     5881 ttgaaacagg cggttgcggg gcaaacgtta ccgtaccgtt caaacaggaa gcgtttcatc
     5941 tggcggacga acattccgac cgcgctttgg ctgccggtgc ggtcaatacg ctggtgtggt
     6001 tggaagacgg cagaatacgc ggcgacaata ccgacggtat cggtttggcc aacgacatca
     6061 cgcaggtcaa aaacattgcc atcgagggca aaaccatctt gcttttgggc gcgggcggcg
     6121 cggtgcgcgg cgtgattccg gttttgaaag aacaccgccc tgcccgtatc gtcattgcca
     6181 accgtacccg cgccaaagcc gaggaattgg cgcggctttt cggcattgaa gccgtcccga
     6241 tggcggatgt gaacggcggt ttcgatatca tcatcaacgg cacgtccggc ggcttgagcg
     6301 gtcagcttcc tgccgtcagc cctaaaattt tccgcgactg ccgccttgcc tacgatatgg
     6361 tttacggcga agcggcaaaa ccgtttttag attttgcacg acaatcgggg gcgaaaaaaa
     6421 ctgccgacgg actgggtatg ctagtcggtc aggcggcggc ttcctacgcc ctctggcgcg
     6481 gtttcaaacc ggacatccgc cccgtcatcg aacacatgaa agccctgtaa ccatgttccg
     6541 catcgtcaaa tggctgattg ccctgcccgt cggcatcttt atcttcttca atgcctatgt
     6601 gtacggcaac atcattacct accgcgccgt cgcgccccat cggactgcct ttatgtcgat
     6661 gcggatgaag cagtttgaac aagaaggtcg cgatgtcgca ctggattacc gctgggtgcc
     6721 ctacaaccgc atttccacca acctgaaaaa agccctgatt gcttccgaag atgtccgttt
     6781 tgccggacac ggcggcttcg attggggcgg cattcaaaac gccatcaggc gcaaccggaa
     6841 cagcggcgaa gtgaaggcgg gcggatcgac catcagccag cagcttgcca aaaacctctt
     6901 cctcaacgaa agccgcaact atctgcgcaa aggggaagag gcggccatta cggcaatgat
     6961 ggaagctgtt accgacaaaa acaggatttt cgaactgtat ttaaactcaa tcgaatggca
     7021 ctacggcgtt ttcggcgcgg aagctgcgtc ccggtatttt tataaaaaac cggccgcaga
     7081 cctgaccaaa cagcaggcgg cgaaactgac ggcactcgtc cccgccccgc tctactacgc
     7141 tgaccatccg aaaagcaaac ggctgcgcaa caaaaccaat atcgtgctca gacgcatggg
     7201 ttcggcagaa ttacccgaaa gcgatacgga ctgattgttt cagatatgga aatgccgtct
     7261 gaactgcggt tcggacggca tatgttttct ggttcttata gtggattaaa tttaaaccgg
     7321 tacggcgtta ccccgccttg ccgtactatg ttgtactgtc tgcggcttcg tcgctttgtc
     7381 ctgatttttg ttaatccact aacctgcccg acagccgcaa agaccgtatc gtgcatggca
     7441 ggtatttgcg gtgcgggact gctgccgaaa acggcaggca aaaaataacc gcttataagc
     7501 ggttttttgg tggccagagg cggaatcgaa ccgccgacac acggattttc aatccgttgc
     7561 tctaccaact gagctatctg gcctgtatct gtctcgttaa gaggtgcgta ttaaaccaaa
     7621 tttcgggacg acacgcaagc ggatttgtcg atatttttta cccaaccgac taagtcgatg
     7681 ttttaagttt aaattaattt cacagaaaaa tagtgcaaat cggttttttg ccgttcgttc
     7741 ggcgatgttc tgctttgcag acggcaaaac gcttcaatat ttgaagttct cgcccaaata
     7801 gacggcgcgc acctgttcgt tgccgaccaa atcgtcgggc ttgccggaag ccagcaccgc
     7861 accgtctgaa atgatgtagg cgcggtcgca gatgctgagg gtctcgcgga cgttgtggtc
     7921 ggtaatcaat acgccgatgc cgcgcgattt gaggaaaccg atgattttct ggatgtcgat
     7981 gacggcgatg ggatcgacgc cggcaaaagg ttcgtccaaa aggatgaaac gtggtttcat
     8041 ggcgaggaca cgggcgattt cgacgcgccg ccgttcgccg ccggacagcg acggcgcagg
     8101 attgcggcgc aagtgtccga tattgaggtc ggcgagcagt ttttcgattt ctctgtcgat
     8161 ttgattttta tctttggttc tgatttccaa gatggcgcgg atgttttgtt cgacggtcat
     8221 tttgcggaat atcgaggctt cctgcggcag gtagccgaca cccaggcggg cgcgttcgtg
     8281 tatgggcagg tggcgcaatt cccgcccgtc cagcatcacg ctgcctgcgt cggcagcgat
     8341 gagcccgact atcatgtaga agccggtggt tttgcctgcg ccgttgggcc cgagcagtcc
     8401 gatgacttcg ccgctttcga tctcaaggga gaaacttttg acgacttggc gttttttgaa
     8461 acttttctgc aggttttgga cgacgaggcg gctgttgttt gcgctcataa atctcttctc
     8521 ggcatttcgg tcggcaaatg ccgtctgaac tgcgtttccg tttcagacgg catcggggtt
     8581 attcggtttt ttgtgtgctt gaaggctgga tgacgacgct gaccctgccg gttttggaag
     8641 cggatttcgc acccgatttc gtgctgccgt tgatggtata gacttcggtt ttggtgttgt
     8701 aggtaatgac cgcaccttcg gcaacgtcgc cgccgcgctg cactttggca ttgccggtca
     8761 gaacgacggt gcttcctgcg gaggaatagg taacgttgtt tgcctgaccg cgcaccgtcc
     8821 ctttgccccc gtccaacgtt tggctgaagc ggacgggcga accttccgcc ctcacggatt
     8881 cgccgccttt gccgccgcgt gtgacgttga cgcgcgaggc ggaaatgttg agcgtaccct
     8941 gtctgatgat gacattgccg ctaaatgtgg tactttggtt ggcttgatcg agcgaacctt
     9001 ggtcggcttc gatttggatg ggccgtctgc tgtcgctttg aagggcaaaa gcgggagaag
     9061 ttgcaaaaat tacaattaaa acaaatagct tacatatctt ttgtatcata aatcgcggct
     9121 ttcactttag atgagaagtt caacatgcct gttttgtggt tgtaggtcat accgcccgcc
     9181 tgaccgtgcg acgcgccata ttggaaactg acaggcgtat cggtttgggc atattgagat
     9241 tcggtatcga cgtgcagttt ttcggtttcg actttacccg cctgccgcct gccgtcggcg
     9301 gttttggtca gcacaacgtt gtttttaaaa agaacctgtt tgttttcggt atggtaaacg
     9361 gcttcatcgc tgccgacttc gtacaacagc ctgccttctt ggaagaacac gagatgcggc
     9421 gaatcaaaat ggatgtcgct gttttcggga aactgtttcg cacctttcgc gctcaaatgt
     9481 tctttcaagt atccctgttc gtcaaaccgc cttccgtcca agccgtccat tgtgtattga
     9541 ggttcgtcgg gattgagcct gacttcctcg atttcgactt cgctgatacg gcccaaccat
     9601 gccgacaggc tgcccaaggc aaccgccaat atcaatggga acgcaattcc gtaccgccat
     9661 cttactttca tttgatgtac ccgttcaaag ccgcgcccaa cgtacctttc gcgcggataa
     9721 tcaggtcaca cacttcgcgc accgcgcccg caccgccggg gcgtttggca atataggcgg
     9781 cgtgttgcag cgtaaaccaa tgcgcgtcgg ggacggcaac cggcaatccg cagcgcacca
     9841 tcaccggcaa atcgaccacg tcgtcgccga caaaggcgca ctcggcttct tccaagcctg
     9901 cctgcgcgcg cagctcttca tacgcggcgc gtttgtccga aatgcctttg aagtagtaat
     9961 ttatgcccaa ctgtttgacg cggatgccga cggagggcgc gtcccgacct gtgataatcg
    10021 cagtctgcac gccgcttgcc tgaagcattt tcaggccgtg tccgtccagt gtgtgaaacg
    10081 atttgatttc ttcgccgtcg tcgcggataa agatgcgccc gtcggtcaaa acgccgtcca
    10141 catccaatat cagcagtttg attttggcgg cgcgtgcctg taattcggga gaaattgcct
    10201 gcattgggat ttccttgttt tcagacggca tatcttacca caaatgccgt ctgaaacggc
    10261 tgcaacgcct tatacaatcc gcgccgccaa aaggtcgtgc atattcagcg cacctgtcag
    10321 cacgccgtct gcatcggtaa ccagaagccc gttcacatgg tttgcctgca tgactttcag
    10381 ggcttcggtg gcgaggcgtt cggcggagat ggttttaggg tgtgtatgca tgacttcgtc
    10441 tatcgaaaga ccggtaaaat tgtcgcattc ttgaaacagg cggcgcaaat cgccgtcagt
    10501 aaacacgcct ttcagacagc cttgcccgtc cgttaccgcc aacatgccca aacctttctc
    10561 gctcatgctg acgatggctc ctttcaaggg cgtgccgagt cggacggcgg gcaggccgcc
    10621 gcctttgtgc ataatgtcgg caacgcgcaa aagcaggcgt ttgccgaggc tgccggcagg
    10681 gtggatcaag gcgaagtcgt cgggcgtgaa cgcgcgggcg cgcagcagga cgaccgccaa
    10741 cgcgtcgccc aaagccataa cggcggtggt gctggtggtc ggggcaagcc ccaacgggca
    10801 ggcttcttgc gaaaccgatg cggtgatgtg gatatcggca tggcgcgcca tggttgaatc
    10861 ggggcgggcg gtgatgcaga cgagggtaat gtctttgcgt ttgagcgcgg ggatgatggc
    10921 ggtgatttcg tcgctttcgc cggaattgga aatggcggcg acgacgtcgt tgtccacaat
    10981 catgcccaaa tcgccgtgtg ccgcttccgc agggtggacg aaaaacgcgg gcgtgccggt
    11041 cgaggccatg gttgccgcca ttttgcgccc gatatgtccg gacttgccca tacccgtgat
    11101 gacgaccctg cccttgcagt gcaacaacgc gtctgccgca aggacgaagt tttcgtccaa
    11161 ttccgccgca atttcgcgca agccttccgc ttcggtgtgc aacacttcgc gcgcccagtc
    11221 gagatatttt tcgttttctg ccattgccat ccgcctttgc tgtaacaaaa ctacccaaaa
    11281 tttccgaatc ggggtagcat aagccttcaa accccgccca tccatccaaa ggaatcaaaa
    11341 atgactattt tatcggacgt taaagcatta ggacaacaaa tctggctgga caacctttcc
    11401 cgctcgctcg tgcaaagcgg cgaattggcg caaatgctca aacaaggcgt gtgcggcgta
    11461 acttccaatc ccgccatttt ccaaaaagcc ttcgccggcg acgcgcttta tgccgacgag
    11521 gtcgccgccc tcaagcggca aaacctcagc cccaaacaac gctacgaaac catggcggtt
    11581 gccgacgtac gggcagcctg cgacgtttgc cttgccgaac acgaatccac cggcggcaaa
    11641 accggtttcg tcagtctcga agtttctccc gaacttgcca aagacgctca aggcacggta
    11701 gaagaagccc gccgcctcca tgccgccatc gcgcgtaaaa acgccatgat taaagtgcct
    11761 gccaccgacg caggcatcga tgcgctcgaa accctcgttt cagacggcat cagcgtgaac
    11821 ctgaccctgc tgttctcacg cgcccaaacc ctcaaagcct acgccgccta cgcgcgcggc
    11881 attgccaaac gcttggcagc cggacaaagc gttgcccata tccaagttgt cgccagcttc
    11941 ttcatctcgc gcgtggacag tgcgctggat gcaacgctgc ccgaccggct caaaggcaaa
    12001 accgccatcg cccttgccaa agccgcctat caagattggg aacaatattt caccgccccc
    12061 gaatttgccg cactggaagc ccaaggcgca aaccgcgtgc agcttttatg ggcatctacc
    12121 ggcgtgaaaa acccagccta tcccgacacg ctctacgttg acagcctgat cggcgtgcac
    12181 accgtcaaca ccgtccccga tgccacgctc aaagccttta tcgaccacgg cacggcgaaa
    12241 gcgacgctga ccgaaagcgc ggacgaagca cgggcgcggc tcgccgaaat tgccgcgctc
    12301 ggcatcgatg tcgaaacctt ggcggcgcgt ttgcaggaag acggtttgaa acagtttgaa
    12361 gaagcctttg aaaaactgct cgcgcctttg gtttaggcat ttccccgatg ccgtctgaaa
    12421 ggctttcaga cggcatcgcc cttttgaccg gacacagtgc ggcatatcaa ggatccgccc
    12481 taaaaatatt aatgggcaag aagttaatgc acgacattac ccgccaacac aaaaggaaat
    12541 agaatcatgg caaaagcact cgaaatcatt tcacccgatg aaatttattc agatctgatt
    12601 tttaaggatc cggtacctcc ccatactata tatacagaat tgatgaaact ggttggtaga
    12661 aatgcaggca atgagagaat tgcagataga gccttcgatt ttttctcgcc tgctcttatg
    12721 gatgattcag ctaccgaaga gcaatataat gccctatatg atttaacctt gcttgaagag
    12781 cccgggatgg aactggataa agatgagata acggctttga ttaatagtct taaataaagg
    12841 ggctgtaccg gatatgtaca caggacgctt cgcccccagc ccgaccgggt tgctccacat
    12901 cggctctctt ctgaccgccg tcgcttccta tgccgatgca cgttcaaacg gcggcaaatg
    12961 gctggtccgt atggaagacc tcgatccgcc gcgcgaaatg ccgggggcgg caagccatat
    13021 cctgcacacg ctcgaggcat tcggattcga gtgggacggc gaagtcacct atcagagccg
    13081 ccgttacgcc ctgtatgaag aaaccctata ccgtctgaaa accgccggac tggtctatcc
    13141 ctgccattgc aaccgcaaag actggcaggc cggggcaagg cggggcacag acgggttcgt
    13201 ctataacgga cgctgccgcc accccggcca acgccctgca ctgcaaggca aacagccgtc
    13261 gtggcgcatc cgcgtccccg accgcgacat cggcttttca gacggcatcg tcggcagtta
    13321 cacccaaaac ctcgcccgcg acatcggcga tttcgtcctg ttccgtgcag acggttactg
    13381 ggcataccag ctcgccgtcg ttgccgacga tgccgaacag ggcgttaccc acatcgtgcg
    13441 cggacaagac ctgctcgttt ccacaccgcg ccaaatctat ttgcagcagt gtttgggcgt
    13501 tccgacaccg caatatgccc acctgccgct gctgaccaac gcgcaagggc agaaatggtc
    13561 gaagcagacg ctcgcccccg cattggattc aaaccgccgc gaacaacttc tccgccaagt
    13621 gttccgctac ctcaagctgc ccgaagcacc ggaaaccgac cgccctgccg aattgctcga
    13681 ctgggcggtg gcacactggg atatggacaa agtgccgaaa cacgccatta ccgcccccta
    13741 aacaccaccc gacaaacgca caaatgccgt ctgaaacctt cagacggcat ttcacacata
    13801 cagccccgcc ctattcgcgc gtatttgccc gttcgacttc tttccacgct tcgaaaatca
    13861 ggccgccgtc gttgtctttc aagagcgttg catcggaaag catacgccgc caagtccgcg
    13921 cgcctttcaa accgtgcatc agcccgaggc tgtggcggac gatgtggcgc aggattgtgc
    13981 cgcgtccggc ttggatttgg acttggctgt atgtgtagag gcgctgcacc aaatcggcgt
    14041 attcgatcgg ggcgcggttg tcaccgtaaa acagcctgtc ccattcgcgc atcaccatcg
    14101 ggttgtggta tgcctcgcgc ccgaccatca cgccgtcaac gtgttgcaag tgtccggcga
    14161 ttgcttcgtt ggtggtgatg ccgccgttga tgatgatttc cagctcggga aactcctgct
    14221 tgaggcggta aacgtaatcg tatttcaagg gcggaacatc gcggttttct ttgggcgaaa
    14281 gaccgtccag ccaagcgttg cgggcgtgga cgatgaaggt tttgcaggcg gttttgtcgc
    14341 gcagcgtgcc gacgaaatcg gcaacggttt ggtattcggt ctgcctgtcc acaccgatgc
    14401 ggtgtttgac ggtaacgggg atgccgaccg cgtcgtgcat ggcgttgagg cagtcggcaa
    14461 ccagcccgac ttcgttcatc agacacgcgc cgaacgcgcc tttctgcacg cgcggactgg
    14521 gacagccgca gttgaggttg acctcgttgt agccgtattc ttcggcggct ttggcggctt
    14581 tcgccaaatc ggacggatcg ctgccgccca gttgcagggc gacgggctgc tcgccttcgt
    14641 taaacatcaa aaaacggtct ttgtcgccgt aaacaatcgc gccggcgttg accatttcgc
    14701 tgtaaagcca agcatttcgg gtaatctggc gggcaaggta acggtagtgc ctgtccgtcc
    14761 agtcgagcat cggggcgacg cataaacgcc ttttcccttt atgttcaata agttccttgt
    14821 ttatcataag gttttcacat tcttttaact caaacattct ttattatttg cttggatttt
    14881 tgcccatttc actatatttg acgtaccata cttgcaccat aatttttaat tcttggaatg
    14941 gtgcaaatgg caacaataac caagcggcgc aatccttccg gcgaaacagt atatcgggtt
    15001 caggtacggg tcggcaagaa aggctatcct gctttcaatg agagcaggac gttcagtaaa
    15061 aaggctttgg cggtcgaatg ggggaagaaa cgggaggcgg aaatcgaagc cggcccagaa
    15121 ctgcttttca agcgcggcaa ggtcaagatg atgacgctgt ccgaagctat gcggaagtat
    15181 cttaacgaaa cgcttggagc gggtcggtca aagaaaatgg gcttgcgttt tctgatggag
    15241 ttcccgattg gcggcatcgg catcgataag ctgaaacggt ctgatttcgc ggaacacgtt
    15301 atgcagaggc ggcgcggaat ccccgaactg gacattgcgc caatcgcggc ttctacggca
    15361 ttgcaggagc tgcaatatat ccgttccgtg ctaaaacacg ccttttatgt gtgggggctt
    15421 gaaataggct ggcaggaatt ggatttcgcg gcaaacgggc tgaaacgctc gaatatggtt
    15481 gcgaaatctg caatcaggga cagattgccg accacggaag aactgcaaac cctgacaact
    15541 tatttcctgc ggcaatggca aagcaggaaa tcttccatac cgatgcacct gattatgtgg
    15601 ctggcgattt atacgtcaag gcggcaggat gagatttgcc gcctgctgtt tgacgactgg
    15661 cacaaaaatg attgtacccg gccggttcgt gatttaaaaa atccgaacgg cagcacaggg
    15721 aataataagg agtttgatat tctgcctatg gctttgccgg tcattgacga gctgccggag
    15781 gaatcggtca ggaagcgtat gcttgccaac aaaggcatcg ccgacagcct tgtaccgtgc
    15841 aacggaaaat cggtttccgc cgcttggacg agggcgtgca aggttcttag aattaaagac
    15901 ttgcgcttcc acgatttacg gcacgaggct gctacccgta tggctgaaga cggcttcacg
    15961 attccgcaaa tgcaacgggt aacgctgcat gacggttgga acagcctgca gaggtatgtg
    16021 agcgtacgca aacgctcgac gcggctggat tttaaagagg caatgatgca ggcgcaaagc
    16081 gatataaaat ccgggaagtg atgttaaatt aaaggggatg tgccgcatcc cctgattcct
    16141 ccgtcttgca tcaagaccgg aatttccgat attccgattt tgccttttct tccttgtctg
    16201 cgattgcctt tgcaagctct cggacgctga cgaaatattc cgattttcgg ctgtcggaca
    16261 atcggaaaat cggaaacgga agctcgcacc gtacccccga tgctttaaac gcgttaaagc
    16321 cgatatgggc aaaaaagtct ttgtgtactt ccctcggcgg gatatacggg cggccgccgt
    16381 atgcgattaa aagcccttgt tctttactga tgttcatata tacctctttc tgttaattct
    16441 gctttgtccg ggtttccgct tttttacggc ttggcggatg ttcagcctgc gtcggtgcat
    16501 aagtcctcct gttcgggcgg ttgttcagcc ggccgcccct ccgaaaagtg gcaggctgca
    16561 cgggaagtag tgtttttttc cgtatttctc tgcctgtatc aggttgcaca ggcggtcgca
    16621 aaagccgtca aattcggcat gcggccagcc ttctatctcg ttgaccattt tgctgaacct
    16681 caattcccgg gcaatccgct ttgccgctgc gtcacggcat tcgtgttcca agaggacggg
    16741 gtcttccttt tccttcggcg gtgcaggttc ttgcacggtt tgccacaagt ccggttcgat
    16801 ttgccaagtg tcggcggcaa gcagggtgtc tgttttgcag tcgtacagtt cgcggatgca
    16861 gccgccgtcg gttttgtctt tgtcgataac cagtaaaagc acttcgatcg gcgtgtcttc
    16921 aaacgcgttt tgtatgcggt tgagttctgc cagccggttg ccgatgattt cgcggaatcg
    16981 ctgctcgctt ttgcggtagg cgatgccggg gaacaggatg aagaatccga agcggcgggc
    17041 gttttccaat ccttttaaaa cgaagatttc atcggctacg cccgatttct tccacgggta
    17101 ttcctgtgcg atacggcttt tttcgtcttc gctcaaatcc tttagcttga tggaaaacgg
    17161 cggattcatg acggtgcagt cttgcggttc gccgtcggta taaaggaaaa ggcttgtgtt
    17221 gtatactttg gcggcagggt agttttgcaa caaggcttta cacgcttccg cctgtatctc
    17281 tacggcgcgg aaatcggacg gttcgatgta ctgctccagc tgcccgcttc ctgccgcgcc
    17341 gtcaaatacg cccgggtgtt cgccgcagta tcggcggact ttggcggcaa ccagccgcct
    17401 caagc
//
LOCUS       DAXDXW010000051        16860 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXDXW010000051 DAXDXW010000000
VERSION     DAXDXW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16860
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.052"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..796
                     /gene="pglE"
                     /locus_tag="ACFB64_001407"
     CDS             <1..796
                     /gene="pglE"
                     /locus_tag="ACFB64_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(galactosaminyl)2-N,
                     N'-diacetylbacillosaminyl-alpha1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglE"
                     /protein_id="HFC1209754.1"
                     /translation="PNTKYIYQDNQGLSEARNTGIKNSNGKYIAFIDSDDFINCQVLL
                     DFLGKDDSDMPDVVFLNAVKYDKGRVSYFGEDYQPEKILNQSKVEVLKGLCRFRKFPG
                     SAWNKIIKRELIIREKLFFEKGIYSEDIEWSMRLFNAATTFSYLDGCYYYYRQGRKDS
                     ITGTVSEKGIKSLLYILEKNAKMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIACDSD
                     IKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
     gene            837..1367
                     /locus_tag="ACFB64_001408"
     CDS             837..1367
                     /locus_tag="ACFB64_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC1209755.1"
                     /translation="MNAIRTFQNRMPEIHETCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDDVMIGAGSLVPPRKRLEGGYLYIGSPVRQVRLLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1535..3130)
                     /locus_tag="ACFB64_001409"
     CDS             complement(1535..3130)
                     /locus_tag="ACFB64_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC1209756.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVEDI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFGIIKGINNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATMRMVGPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHNRELAEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3237..3632)
                     /gene="hisI"
                     /locus_tag="ACFB64_001410"
     CDS             complement(3237..3632)
                     /gene="hisI"
                     /locus_tag="ACFB64_001410"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC1209757.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDTIVMLIAQNGGIACHTGRES
                     CFYKKWNGGAWETADAVLKDEKEIYGSTH"
     gene            complement(3663..4430)
                     /gene="hisF"
                     /locus_tag="ACFB64_001411"
     CDS             complement(3663..4430)
                     /gene="hisF"
                     /locus_tag="ACFB64_001411"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC1209758.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLINEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRTMREAGIEVRL"
     gene            complement(4443..5180)
                     /gene="hisA"
                     /locus_tag="ACFB64_001412"
     CDS             complement(4443..5180)
                     /gene="hisA"
                     /locus_tag="ACFB64_001412"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC1209759.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPADTALHWFEQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGMRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDLVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQSVRIPVIASGGLTGLDDIPALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5213..5851)
                     /gene="hisH"
                     /locus_tag="ACFB64_001413"
     CDS             complement(5213..5851)
                     /gene="hisH"
                     /locus_tag="ACFB64_001413"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC1209760.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTKIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFARDLRDPQGCRLKVPHMGWNTVRQTQNHPLFQGIPQNTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(5981..7483)
                     /gene="pta"
                     /locus_tag="ACFB64_001414"
     CDS             complement(5981..7483)
                     /gene="pta"
                     /locus_tag="ACFB64_001414"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC1209761.1"
                     /translation="MAKVLVVPVSAGLNTSAAAQAFAKALDAQVFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NTDADALAHQLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQAGKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSTNVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7668..8726)
                     /gene="fbpC"
                     /locus_tag="ACFB64_001415"
     CDS             complement(7668..8726)
                     /gene="fbpC"
                     /locus_tag="ACFB64_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC1209762.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIIGASGCGKT
                     TLLRCLAGFEQPDSGEISLSGKTIFSKNTNLPVRERRLGYLVQEGVLFPHLTVYRNIA
                     YGLGNGKGRTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCRLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPAASIHAVVLKTTPKARHTEISLRAGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8747..10264)
                     /gene="fbpB"
                     /locus_tag="ACFB64_001416"
     CDS             complement(8747..10264)
                     /gene="fbpB"
                     /locus_tag="ACFB64_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC1209763.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLTAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LILGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTADDVHTLSTAVWEYTSDAQYAAAAPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10332..11327)
                     /gene="fbpA"
                     /locus_tag="ACFB64_001417"
     CDS             complement(10332..11327)
                     /gene="fbpA"
                     /locus_tag="ACFB64_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC1209764.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYVPT
                     SGAFLEQIVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLAGKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11579..12059)
                     /locus_tag="ACFB64_001418"
                     /pseudo
     CDS             complement(11579..12059)
                     /locus_tag="ACFB64_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
     gene            complement(12117..13493)
                     /gene="argH"
                     /locus_tag="ACFB64_001419"
     CDS             complement(12117..13493)
                     /gene="argH"
                     /locus_tag="ACFB64_001419"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC1209765.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDQRLAEWDIQGSLAHAQ
                     MLTRSGVLSENDLTDIRRGMSEILEEIRSGKIEWPLDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQNLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHLLAYVEMLGRDFERMADCRKRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPGNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEALQGFSKLISDDVYGVLTPEGSLNARNHLGGTAPEQVRLQV
                     KRWREMSA"
     gene            complement(13512..14381)
                     /gene="galU"
                     /locus_tag="ACFB64_001420"
     CDS             complement(13512..14381)
                     /gene="galU"
                     /locus_tag="ACFB64_001420"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC1209766.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKANPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETKLEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14409..15008)
                     /gene="rdgB"
                     /locus_tag="ACFB64_001421"
     CDS             complement(14409..15008)
                     /gene="rdgB"
                     /locus_tag="ACFB64_001421"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC1209767.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFKPYGITVLPQSPFGIPECP
                     EPYPTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCSYVCVLVFVRHKDDPRPIIAEGIWHGQWHDTPLGQNGFGYD
                     PYFYLPEHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(15001..15234)
                     /locus_tag="ACFB64_001422"
     CDS             complement(15001..15234)
                     /locus_tag="ACFB64_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC1209768.1"
                     /translation="MNRRKIYLLSVALFTLAFMLPVLLGAYLLTAGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAPSSSQGNRYV"
     gene            complement(15342..15875)
                     /locus_tag="ACFB64_001423"
     CDS             complement(15342..15875)
                     /locus_tag="ACFB64_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC1209769.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RIEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGGAE
                     EAKKVIKESIERWNKQA"
     gene            complement(15977..16435)
                     /gene="nudB"
                     /locus_tag="ACFB64_001424"
     CDS             complement(15977..16435)
                     /gene="nudB"
                     /locus_tag="ACFB64_001424"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC1209770.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLEDGQLQDRHDSTVYEIYHHWRHRYPKGVFENREHVFR
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16646..16722
                     /locus_tag="ACFB64_001425"
     tRNA            16646..16722
                     /locus_tag="ACFB64_001425"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16680..16682,aa:Pro,seq:ggg)
BASE COUNT         3445 a         4030 c         5187 g         4198 t
ORIGIN      
        1 accaaatacc aaatatatat atcaggataa ccaaggattg tcggaggcga gaaataccgg
       61 aataaaaaat tcaaatggaa aatatatagc atttattgat tcggatgatt ttattaattg
      121 tcaggttttg ctggattttc ttggtaaaga tgatagtgat atgccggatg tggttttttt
      181 aaatgcagtt aaatatgata agggaagagt ttcatatttt ggcgaagatt atcagcctga
      241 aaaaatatta aatcaatcca aagtcgaagt tttgaaagga ttgtgccgat ttagaaaatt
      301 tccgggttcg gcgtggaata agattataaa aagagaattg attattagag aaaaactgtt
      361 ttttgaaaag ggaatttatt ctgaagatat tgaatggtca atgaggttgt ttaatgcggc
      421 aacaactttt tcttatttgg acggttgtta ttactattat cggcagggaa gaaaagattc
      481 tattacggga actgtttcgg aaaaaggtat aaagtcatta ttatatattt tggaaaaaaa
      541 tgcgaaaatg gaatttaata gggatatatc gagttatctt tattcttttc tttcctacga
      601 atatctcgtt ttgcttttta taatgacgag taaaaatata gcgtgtgatt ctgatataaa
      661 aagaagggca tatcatttaa ggtttatgct gttaaagtcc aataagctga tatataagct
      721 gatattcccg ataatcacat tattcggggt cgatattaca ggcaggattt taaaagcaat
      781 cagggggaat atttaataaa tcctttaaca atatatacct taccgaagga ggaaaaatga
      841 acgcaatcag aactttccaa aaccgcatgc ccgaaatcca cgaaacctgc atgatagacg
      901 aggcgtgcgt cgtcatcggc gaagtctcgc ttgccgaaga tgtttccgtg tggccgtgcg
      961 ccgtgttgcg cggcgatgtg aacagcatca ccgtcggcgc gcgcagcaat atacaggacg
     1021 gcagcgtcct gcacgtttcc cacaaaaccg ccgccaaacc cgaagggtcg ccgctggtta
     1081 tcggcgaaga cgttaccgtg gggcacaaag tgatgctgca cggctgccga atcggtaacc
     1141 gcgtcttggt cggtatgggc agcaccgttt tggacgatgc cgttattgaa gatgacgtga
     1201 tgatcggcgc gggcagcctc gttccgccgc gcaaacgttt ggagggcggc tatctttata
     1261 tcggttcgcc tgtgaggcag gttcgtctgc tgaccgatga ggaaaaagcc tttttgaaat
     1321 attccgccgc acattatgtg aagctgtcga aacagtacgg gatgtgaaat cacatcggcg
     1381 ttcttgcgtc agtcccaaat tcacgcggtt gggatgcccg ataacggtat ccgatgcgcc
     1441 ttgattttga ccggctgcgt ttgagttgca ggcaaaaatg ccgtctgaaa gccttttttt
     1501 cggcttcgga cggcatttta ttgccgattt ctttttagag tttgaccgaa tgttcgcgcg
     1561 tttcgtggaa cacgatgtcc ggccaacgtt cttgcgtcaa ccccaaattc acgcggttgg
     1621 gggcgaggta ggcgaggttg ccgcctgcgt cgatggcgag gttgcctgcg ttggcttttt
     1681 caaattccgc cagttttttc ttgtcgtcgc acgataccca gcgcgccgac cagatggatg
     1741 cgctgtcgaa cacggcttcc acgccgtatt cgttggcgag gcgtgaggtt acgacttcaa
     1801 actgcaacac gccgaccgca cccaaaatca aatccgcgcc gctcatcggt ttgaatactt
     1861 gaaccgcacc ttcttcgccg agttgttgca aacctttttg cagttgtttg attttcagcg
     1921 ggtttttgat gcggacgctg cggaacagtt cgggtgcgaa gaatgggatg ccggtaaacg
     1981 ccagttgttc gccttcggag aagctgtcgc cgatttggat gttgccgtgg ttcgggatgc
     2041 cgatgatgtc gccggcgtag gcttcttccg ccagttcgcg gttgtgcgac atgaaggtta
     2101 ctacgctgga ggcggcgatt tcgcggttga tacgcaggtg tttcatcttc atgccgcgct
     2161 cgaatttacc ggagcagacg cgcaagaagg cgatacggtc gcggtgtttc gggtccatat
     2221 tggcttggat tttaaagata aatccggaaa atttcggctc gtccggcccg accatgcgca
     2281 tggtcgcgtc gcgcggtttc ggtgcgggtg cccagtcaat caatgaattg aggatttcct
     2341 gaatgccgaa gttgttaatc gcagagccga agaacactgg cgtgagttcg ccggcgagaa
     2401 attcgtcgag attaaattcg ttggaagccg cctgcaccaa ttcgatttcg tcgcgcaact
     2461 gctggatttc caacggaaag cgttgttcca attcgggatt gtttatgcct ttgatgatgc
     2521 cgaactcgtg cggcaggcgt tcgccgcccg cttcaaagag atagatttcg tcgttcagga
     2581 tgtggtacac gcccttgaag tttttgccca taccgatcgg ccaggtaacg ggcgcgcagc
     2641 ggatttgcag gatgtcttcc acttcgtcca agagttccaa agaatcgcgc acttcgcggt
     2701 cgtatttgtt catgaaggta acaatcggcg tatcgcgcag gcggcagacg ttcaagagtt
     2761 tgatggtttg cgcttccacg ccttttgccg cgtcgatgac catcaaggcg ctgtccactg
     2821 cggttaaaac gcggtaggtg tcttcggaga agtcttggtg tcccggcgtg tccaagaggt
     2881 tgacggtgtg gtctttgtag tcgaactgca tcacgcttga tgccacggaa atgccgcgct
     2941 gcttctcgat gtccatccag tcggaggtgg cgaatttgcc ggttttctta cctttcaccg
     3001 tgcctgcgct ttgaatcgcg cccgaaaaca gcagcagttt ttcggtcagc gtggttttac
     3061 ccgcatcggg gtgggagatg atggcaaacg tgcggcggcg gcgcacttgg tcgaggattt
     3121 cttgggacat ggttttcttt gcaaaaaggt tcaggccgct tttcagacgg cccggacagt
     3181 gtttgagacg gcgaaattgt acaaaaaaat gcctgataat tcaatgttgg aggcggtcag
     3241 tgcgtgctgc cgtaaatctc tttttcgtct ttcaggacgg catcggcggt ttcccacgca
     3301 ccgccgttcc attttttgta aaagcagctt tcgcgcccgg tgtggcaggc gatgccgccg
     3361 ttttgggcga tgagcatcac aatggtatcg ccgtcgcagt cgaggcgcag ttcgcggact
     3421 ttttgcgtgt gtcccgactc ttcgcccttc atccattgtt tttggcgcga acggctgtaa
     3481 tagtgggcaa agccggtttc gacggttttt tgcagggctt cggcgttcat ccacgccacc
     3541 attaaaacac gtttggtttc ggcatcttgg gcgatggcgc aaaccaaacc tttttcgtca
     3601 aatttgacgg cttcaagcag gtttttatcc atatttcctt tcagacggca tagtcggggc
     3661 ggtcagaggc gcacttcgat gccggcttcg cgcatagtgc gtttggcttc gcggatggcg
     3721 atttccccga aatggaaaat gccggcggca agtacggcat cggctttgcc ttcggttatg
     3781 ccttcaatca ggtgccggac attgccgacc ccgccggagg cgatgacggg gatgtcgacg
     3841 gcttcggcaa cggcgcgggt cagcggcagg ttgaaaccct gtttcgtccc gtccctgtcc
     3901 ataccggtga gcaggatttc gcccgcgccg cgtttttgca tttcgaccgc ccattccacc
     3961 gcatccaaac cggtcggatt gcgcccgccg tgggtaaaga tttcccagcg tgtgttttcg
     4021 gggttgacgg ctttggcatc gacggcggcg acgatggctt gcgaaccgaa aaatccggcg
     4081 gcttcgttaa ttaaatcggg acgggtaacg gcggcggtgt tgatgctgac tttgtccgcg
     4141 ccggcattga gcaggcggcg gatgtcggca acggtgcgta cgccgccgcc gacggtcagc
     4201 gggatgaaga cttgtccggc aacctcttcg atgatgtgca ggatggtgtc gcggttgtcg
     4261 gatgaggcgg tgatgtcgag gaaggtcaat tcgtccgcgc cttcgccgtt gtagcgtttg
     4321 gcggcttcga cggggtcgcc cgcgtcgcgc aaaccgatga agttcacgcc tttgacgacg
     4381 cgcccgtctt ttacgtcgag acaggggatg atgcgttttg ccagtgccat aatcggatgc
     4441 ctttagtcga gggaatctgc cagttgctgc gcttgggcaa aatcgatgct gccctcgtaa
     4501 atcgcgcggc cggtaatcgc gcctgctacg ccatgttttt cggcggcaca cagggcgggg
     4561 atgtcgtcca agccggtcag tccgccggag gcgatgacgg gaatgcggac ggattgggcg
     4621 agtttgaccg tcgcgtcgat gttcacgccg ctcatcatac cgtcgcgccc gatgtcggtg
     4681 tagatgatgc tgttgacacc gtcgtcttca aagcgttttg ccaaatcaat tacatgatgc
     4741 ccggttacgg ttgcccagcc gtcgatggcg gccataccgt ctttggcatc cagtccgacg
     4801 ataatcctgc cggggaaggc tttgcacgct tcgcgcacca agtcggggtt tttgactgcc
     4861 gccgtgccga taatcacgtc gttcagtccc aaatccaaat attgtccgat ggttttcaaa
     4921 tcgcgcatgc cgccgccgag ctgcacgggg atgtctttgg cgacggcggc gaggatgtct
     4981 ttgatggcgg gcaggttttg cggaacgccg gcaaatgcgc cgttcaaatc taccaggtgc
     5041 aggcggcgcg cgccttgttc gaaccagtgc agcgcggtgt cggcgggcga atcggaaaag
     5101 acggtcgcct cttccatcag cccttgtttc aagcggacgc aacgtccttc tttcaaatcg
     5161 atggcgggta tcagcagcat agtttttctc cttgtgcggg gccgtgtcgg gcttaccagt
     5221 ttaaaaagtt tttcaacatc gtcagcccgg catcgtggct tttttcggtg tgaaattgcg
     5281 tggcgaatac gttgtctttg ccgacgatgc aggcaaacgg ggacgggtag tcgctttcgc
     5341 ccaatatggt ttcgggattt tcgggggcga aatagtagct gtggacgaaa taaaaacgcg
     5401 tgttttgggg gatgccttga aaaagcgggt ggttttgggt ttggcgcacg gtgttccagc
     5461 ccatatgcgg gactttcaga cggcatccct gcgggtcgcg gaggtcgcgg gcaaagcgtc
     5521 tgactttgcc gccgaaccag cccaagccgt cggtgtttcc ttcttcgctg tggtcgaata
     5581 aaagttgcgc accgacgcag attccgaaaa acggtttgtt ttttaaggca tctttgactg
     5641 cctcgtccaa gccgccgcgc gtcagtgccg ccatacagtc gggcatcgcg ccctgcccgg
     5701 gaaaaatgac tttgtcggcg cgggacacgc ggtcggggtc gccgcttaaa aagattttgg
     5761 tattttttcc ggcaagctgc cccgccgtcc ggacggattt caatacggaa tgcaggttgc
     5821 ccataccgta atcgataatg gcggtttgca tggcttcctc ctcttttttg caatatggct
     5881 gcgattttaa caaacaaatg tgccgggctg ataaaaatgc cgtctgaaaa cgggaatctg
     5941 tcttcagacg gcatagggtt caaacccgga aagccgtttg tcagccttcc atttgttttg
     6001 cctgaacggc agtcagggcg atggtgaaca cgatgtcttc caccagcgcg ccgcgagaca
     6061 ggtcgttgac cggtttgcgc aggccttgca gcagcgggcc gacgctcaag acgttggtac
     6121 tgcgttggac ggctttatag gtgcagttgc cggtgttcag gtcggggaag accaaaacgg
     6181 ttgcctgtcc tgccaccggg ctgcccgggg ctttggattt gcctacgccc ggcacggttg
     6241 ccgcgtcata ttgcagcggg ccgtcgatgg cgaggtcggg gcgtttttcc cgggcaagtt
     6301 tggtggcttc gatgacggta tcgacatcgg ggccgctgcc ggagttgacg gtggagtagg
     6361 aaatcatcgc cactttcggg tcgatgccga aggcttttgc ggaatcggca gactggatgg
     6421 cgatgtcggc aagctgttgc gcggtcgggt tcggattaac cgcgcagtcg ccgaacacca
     6481 gaacctgatt tggtagcagc ataaagaata cgctggatac gaggcttgcg cccggtgcgg
     6541 ttttaatcag ttgcaaagcg gggcggatgg tgttggcggt ggtgtgaacc gcaccggata
     6601 ccaaaccgtc cacatcattt tgcgccatca tcatcgtacc gagtaccacg gtgtcttgca
     6661 gttgcttgcg cgcgtcttcg ggtgtcaggc ctttggattt gcgcagttcg cacatcggct
     6721 cgacgtattg ttcgaccaat gaggcgggat cgatgatttc caaagagtcg ggcaggctga
     6781 tgccgcgttc tttggcaacg gcttcgactt cttcgcgttt ggcaagcagg acgcagcggg
     6841 caatgccttt ttcgtggcag atggcggcgg cttggacggt gcggggttct gcgccttcag
     6901 gcaggacgat gcgtttgccg gcttggcggg cgaagtcgat caggttgtag cggaattgcg
     6961 ccggcgacag gcgttttgct tcgcggcctg ccaatacgga tacgtctttc agcgcgtcgc
     7021 tcgaaccgaa gaaggtcagg ccggttttct cggctgccgc ttcggcaacg gaggctgccg
     7081 caccgtccac gacaaaacct tccaatacgc ccggcgcggc ggcgaagaac tgtttggcaa
     7141 ggttcaattg gtgtgccagc gcgtcggcat cggtattgtc ggaacggacg gcgaagacgg
     7201 ctgccgcgtc aagggacaat gccagttcga cgtttttgcc tgcgagatag attttgtcgg
     7261 catcgggcgc gatgccttcg atgacgaggt tggcggcatc gagtgcggca actttgccga
     7321 ccagtgcgtc gaaccagtcg tcgcttttgc cttgcgcgag cagggtttcg gcggttgcgt
     7381 caacggcttg gaaaacttgt gcgtccagtg cttttgcaaa ggcttgtgcg gcggcggagg
     7441 tgttcagtcc ggcagatacg ggtacgacga gtacttttgc cataatatat cctttcgtat
     7501 gctgcggtgt gcggcatatg tggttggaag gggcggcata tgggcagaaa cggctgcctg
     7561 cgtgccgtgc gtgccgtgtt tggcttgggg cgcgcaggtt gaatatagca aacaaattct
     7621 gtttccaaca agataaatat ccgcaggctt gtggatgctg ccgcctttca gagggtattt
     7681 ccggggaaga acagggcggg accgtccaaa tggaggacgg cggaaatgcc gtctgacagg
     7741 gtgggggcgg aagggaggtt gagcgtgagg acggtttgtc cggccctgag gctgatttcg
     7801 gtatgccgcg ctttgggcgt ggttttgaga accacggcgt gaatggaggc ggcgggtgcg
     7861 gaatgggggt gaaggctgaa ctgttccgga cggatgagca gtgtaccgcg cgtgcctgcg
     7921 ggtgcgccgc tttggacggg caggcggccc aatctgcaat cggcggtgcc gtcggcgttg
     7981 agcgcggcgg ggaacacgat gccttcgccg ataaacaggg cggcatcaag gtcggcaggt
     8041 tgtcggtaca attcgtgagg gcttgcggtt tggaggatgc gcccctgttt catcacggca
     8101 atccggtcgg cgtattgcag ggcttcttcg cggtcgtggc tgacaaaaac ggcggatttt
     8161 ccgttggcgc gcagggcggc aatcatgtct tcgcgaatct ggcggcgcaa ctgttcgtcc
     8221 agcgcgctga agggttcgtc caacaaaatc agttcggggt cgggggcgag ggcgcgggcg
     8281 agggcgacgc gctgttgttg tccgcccgaa agttcgtgcg gatagcgtcc ggcaagttcg
     8341 gaaatgccgg tcaattccaa catggcttcg atgcgctgtc gctcttgcgc cgtcctgcct
     8401 ttgccgttgc cgagaccgta ggcgatattg cggtaaacgg tcaggtgggg gaacagcacg
     8461 ccttcctgta cgaggtaacc caaacggcgt tcgcggacgg gaaggttggt atttttcgag
     8521 aagatggttt tgccggaaag cgaaatttcg ccggaatcgg gttgttcgaa accggcaagg
     8581 cagcgtaaaa gggtggtttt gccgcagccg gacgcgccga tgataaagag aatttcgccc
     8641 gggtcgaggc tgagcgaaat gtcgtttaaa accggggtgt tttgaaaact tttggacagg
     8701 tgtccgatgt gcagggcggc ggtcatagcg gtgcttcctc aagctgttat ttgaaggcgt
     8761 atttcttcag caggaatacg ggtatgccgg aaaataatac cagcatcagc gcgtaagggg
     8821 cggcggcggc gtattgtgcg tccgatgtgt attcccaaac ggcggtggag agcgtgtgga
     8881 catcgtcggc ggtcagcagc agggtggcgg tcaactcttt catcagcttg aggaagacga
     8941 gtgcgaatgc ggcggtaatg ccgggcagga tggacggcag taccaacgtc ctgaaaataa
     9001 agaagtgtcc gcgccccaat gttgcgccga cctgttccat ccctttgggg agttgttcca
     9061 aggaagtcct cagggtggtt tgcgccatcg gcaggtaaag catgaaatag gcaaggatga
     9121 cgacgataaa ggtttggtaa acggcagggg tgtagttgat gctgaaataa accaaggata
     9181 gggcgataac caaaccgggg acggcgtgca gtaaaaacgg cagcctgtct atccaaacgg
     9241 ttaaaaaatt gcgatagcga accgatgccc aaacaagggg caaggcacat aatatagtca
     9301 aaatcgcacc taaagccgat acgcttaagg aacggataaa ggcatcaaat acggatacga
     9361 gcgcgaatgt gccggaagtg ccgaccatca tccaatgtat caatacgcca aaggggataa
     9421 taatgcccaa aatcaacaag ctgcttaaaa aaacaatcgc gccgatctga ccgggcagtt
     9481 tgagggtttt gacgggataa ggacgggcaa cgcctttgcc gctgtggtaa atcttggctt
     9541 tgccgcgaaa tatgctttct ccaaatacga cgatgccgca caccgccgtc aaaacagcgg
     9601 aaagcagggc ggcggtattg ttgttgtagg acatttcgta ttcttggaaa atggcagtgg
     9661 taaaagtggg gtagttcaaa atggataccg cgccaaattc gaccagcata tgcagggcga
     9721 tcagtaacac gctgctgccg atggcgggtt tgagctgcgg gaggatggcg gaaaaaaagg
     9781 tttgcaggcg gcttttgccc aaggacaggc tgacttcttc gtaagacagg ctgatgcgtt
     9841 tgagtgccgc ctcgacgggc aggtaggcga gcggaaacga ggacaggctc ataatcatca
     9901 ctgtccccca aaagccttcg acgcggaagg tcaggctgat ccaggtgaaa cagctgacaa
     9961 atgcggggat gcacaaaggc agggtgattg ccgtctgaaa aaaggttttg ccgaagaagc
    10021 ggtaacgttg gaacaaaagg gcgcaggcaa tgcccaaaac aatggaaatc agggtaacgc
    10081 ccgccatcat cgtcaaggtg ttggagagca aatcccacat acgcgggcgg aacaacagtt
    10141 cgacggcgcg gttgatgccg acctgccacg aacgcatagc gacatataaa aaaggcaggg
    10201 taagcggcag ggcgatcagt aggatgaggc cggtaagcca aatgggtatt tttttaggag
    10261 acatagtgtt tttcatcggc aaaacgggcg gacagtataa atgtccgccc gtttgacaat
    10321 ccgaaaacgg cttatttcat accggcttgc tcaagcagcc gggtggcgtg ttctttttcg
    10381 gaaacagtgg tggcggacac ttggggtgct tccaacttgg cgatgggttc caaattgaag
    10441 gtggatacca cgtgcggatt caaaggatat tcggcacgga cggcggtcag ggcgcgctgt
    10501 ccttccttgc cggcgaggaa ggcgacgaat tttttcgcct catccttgtt ttgggaggat
    10561 tttaacacgg ctgcgccgga ataggtaacg agtgcgccgg gatctctgtg gcggacgaaa
    10621 ttcaggcggg tgtggacatt ttgtacgcct ttttcacgcg cgaaagcgtg ccagtagtag
    10681 ttgttgatga gggcggcatc gatttcgccg ttttcaaccg cttgaagggc gacggagttt
    10741 ttagcgtaag gcttgccgta ttctttcagg cctttgagcc atttcaatgc ggccgcttcg
    10801 cctttcagtt tgacgatggc gacaatctgt tccaagaatg cgccggaagt ggggacgtaa
    10861 ccgatgcggt ttttccattt cggcgtggcg taattcagga cggatttttc caaatctttt
    10921 tcagacagtt tgcgggtgtc gtaaacgacg acgcgcgaac gtccgctcag tgccacccag
    10981 tcttttttgg cggcaaccgg cacgcctttg ccgcgtgttt cgttgatggt ggaggcgggc
    11041 aggggctcta ggaggttggc tgcggaaagg gtggcgagtg ccgggatttg ttcggaatag
    11101 aatacgtcgg cggggcttcg gctgccttct tctttgattt ggccggcaag ctggtcgcct
    11161 ttggcactgt tgagtttgac tttgatgccg gtagcccggg taaaggcatc tgcaacggct
    11221 tgtgccgctt ctttgtgttg gccgttgtac acggtaatgt ctgccagcgc gggggtggcg
    11281 gcggtcaggg ctgcggcaag cagtgcgtat cggatagatg ttttcatatc gattttctcc
    11341 taaatgaatg agggtgtata ccttgttaag acataacggg gtgtagtgta ttccttcttt
    11401 tttataaatg caaataatta ttttttaaat ttgttattat ccgatccggt tattgtttgt
    11461 tctgacttgt attttttccg tgcatcgcgc ccgtaaggcg gaagcggcgg gcaatgcgtg
    11521 gcggaatgtg ggtaaaggcg gcattttgat ttgtcggaat gctggagaac ctctcccttt
    11581 aaaacgccgt ctgaacaagg ttgccggaat agtattgcca tcccggcaga tacagtttgt
    11641 cgggatcttg ccaatattgt tcatccagac tgttcggcag cgaggcggtt ttgttgtcga
    11701 gatgttttgt taatccacta tatatcccac atttctttta ggtttttacc ttccgattgg
    11761 aggtggcgga tttcttttcc caatgcgtct gccgcccgat ggatcagcat tgcgctgtgt
    11821 accgcgccga tttttttcag aacggaacaa gtcttttcgg cggcgggaag accccagttg
    11881 cagacaaatt gcagtatgcc gccgttgccg atgtcggctt ccgcccgcca aatcaaaacc
    11941 agctcctgtt cccgtccgtc catgctttcg agtttgccgt catgctgttc aaaaagtttg
    12001 tccaccgcct gccgcatcat ctgctcgaag cggtcggctt gggtatccgt atcggtcata
    12061 gtattcttgc cttttaaaaa tgccgtctga acatttcttc agacggcatt tgggggttaa
    12121 gctgacattt cccgccagcg tttgacttgg aggcgtacct gttcgggcgc ggtgccgccc
    12181 aagtggttgc gcgcgttgag gctgccttcg ggtgtcagaa cgccgtacac gtcgtctgaa
    12241 atcagcttgc tgaagccttg cagggcttcg agcggcaatt cgctcaaatc aacgccggct
    12301 tcatcggcgt ggcgtacggc ttgggcgacg acttcgtggg catcgcggaa gggcatacct
    12361 tttttgacca gataatccgc caagtcggtg gcggtggcaa aaccctgcat cacggcggcg
    12421 cgcatattgc cgggtttgac ggttacgccg cgcatcatat cggcgtaaat ccgcagcgtg
    12481 tcgatgagcg tgtcggcggt gtcaaacagc ggttctttgt cttcctgatt gtctttgttg
    12541 tatgccaagg gctgcgattt catcagggta atcaggccga taaggtgtcc gatgactcgg
    12601 ccggatttac cgcgcacgag ttcgggtacg tcggggtttt tcttttgcgg catgatggac
    12661 gaacccgtac agaagcggtc ggcgatgtcg ataaagccga aacgcggact catccacaaa
    12721 atcaattctt cagacaggcg gctcaagtga accataacca gcgaggcggc ggcggtgaac
    12781 tcaatggcaa aatcgcggtc ggatacggca tcgagcgagt tctggcagat ttgttcgaaa
    12841 cccaacaatt cggcggtgat ttcgcgctga atcggatagg tcgtaccggc aagggcggca
    12901 gcgccgagcg gcatacggtt gacgcgtttg cgacagtcgg ccatgcgttc gaaatcgcgt
    12961 ccgagcattt cgacgtaggc gagcaagtgg tgtccgaagc tgaccggctg ggcgacttgc
    13021 agatgggtaa aaccgggcat gacggtttcg gcgttttgtt ccgccaaatc caataatgcc
    13081 gtctgaaggt tttgaatcag gttttggata acggtaatct ggtcgcgcag ccaaaggcgg
    13141 atgtcggtgg cgacttggtc gttgcggctg cggccggtgt gcaggcgttt gcccgcgtcg
    13201 ccgattttgt cggtcaggcg gcgttcgatg ttcatgtgga catcttccaa atcgagcggc
    13261 cattcgattt tgccgctgcg gatttcttcg aggatttccg acatgccccg acggatgtcg
    13321 gtcagatcgt tttcactcag cacacccgac cgggtcagca tctgcgcgtg ggcgagcgag
    13381 ccttggatgt cccattcggc aaggcgttgg tcgaaaccga tggaggcggt gtattgtttg
    13441 acgagttcgg aaacgggttc gttgaaacgt ccggaccagg ttttgtcgtg cataaggatt
    13501 ccttgatggg gttattcggt gcggtatttt tccaaaaggc ggcggaaggg ttcgccggtt
    13561 tcggggtgtt ttaaaccgta ggcgacggtg gcttcgagat agccgagttt gctgccgcag
    13621 tcgtagcgcg tgccttcaaa ggggtgcgcc aagacaaatt cgtgatcgag cagcttggcg
    13681 atgccgtctg taagctggat ttcgttgccc gcgccgcgcg gcaggccggt taaaaggtcg
    13741 aaaatgcgcg gggtgaggat gtagcgtccg acaacggcaa ggttggaggg cgcgtcttcg
    13801 ggcttgggtt tttcgacaat gccggtaatg cgttggaact gtttgagctg ttcggtttcg
    13861 acgatgccgt atgagccggt ttgcgacggt tcgacggttt ctacgcccaa aatgctgtta
    13921 ccgctgcgcc cgtacacttc gaccatttgt ttgagcgcgc ctttgggcgc atcgattaag
    13981 tcgtcggcaa ggatgacggc gaagggttcg tcgccgatgg cggcgcgggc gcacaagacg
    14041 gcgtgtccca atcccagtgc ttccgcctga cggatgtaga ggcaggtaat gttcggcggc
    14101 aggatgttgc ggacgtgttc caacaatttg tctttatggc gcatttccaa cttagtttcg
    14161 agttcgtatg ccttgtcgaa atggtcttcg atgctgcgct tgttgcgccc ggtaacaaac
    14221 accatttccg tgcagccggc ttccacggct tcttctacgg cgtattggat cagcggcttg
    14281 tcgacgatgg gcagcatttc tttcgggttg gccttggtgg cgggcaggaa gcgggttccc
    14341 atccctgcaa cggggaagac ggcttttttt atcggtttca ttctttttcc tttgtattgt
    14401 tttgatgttt aaagggcgag tttgcggatt aaatcggcga gtgcctgcgc gcggtggctt
    14461 tcgcggtttt tgacctccga atccaattcg gcggcggttt tgccgtgttc gggcagataa
    14521 aaatacgggt cgtaaccgaa accgttttgc ccgagcggcg tgtcgtgcca ttgtccgtgc
    14581 catatgccct cggcgataat cgggcgcggg tcgtctttat gacggacaaa aaccaatacg
    14641 cagacataag aacaactttt gtcggccttg ccggcaagtt cggcggcaag tttcaggttg
    14701 ttggcggtat cggatttggg attgctgccg gcgtaacgtg cggaatggat gcccggcgcg
    14761 ccgtttaggg cggcggcaca gatgccgctg tcgtcggcga gtgcgggcag cccgctgtgt
    14821 ccggcggcgt gccgggcttt tgccagcgcg ttttcgacaa aggtgggata gggttcgggg
    14881 cattcgggta tgccgaatgg ggattgcggc aatacggtga tgccgtaagg tttgaataag
    14941 ttgccgaatt ctttgagctt gccggcattg ccgcttgcca aaacgatttt ttccggtttt
    15001 tcagacatag cggtttccct gtgatgaaga tggggcggcg cgtagggatt tgtgccgcag
    15061 gtagagggcg agtgcgccga tttgcccgaa cagcgcgccg aatgcgaaaa ggaaggacgc
    15121 gacggcgaag gctttgctgc cggcggtcag cagataagca cccaagagga cgggcagcat
    15181 aaatgccagt gtgaacaagg caacagacaa cagatagatt tttcggcggt tcatggcgtt
    15241 cggtcggaaa cgggatgttc ggattatagc cgattaggac ggcattccct agaggctgga
    15301 aaaatgccgt ctgaagcggc gttcagacgg catggcgggc attatgcctg tttgttccaa
    15361 cgttcgatgg attctttgat gacttttttc gcttcttccg caccgcccca ggattcgact
    15421 ttggtcgtac ccgctttttt caggtctttg tagtggttga agtggaactc gatttgtttg
    15481 atgagctgtt gcggcaaatc ggacaaagtt ttgtaggcgt tgccgttatt gcggtcgtcg
    15541 gcaggtacgc agacgatttt gtcgtccact tcgccgtcgt cgacgaattt catcacgccg
    15601 ataacgcgcg cttccaagaa tacgccggtt gccagaggtt gttcggtaac gagcaacacg
    15661 tccaattcat cgccgtcttc gtccaaagtt tgggggatga agccgtagtt ggtcggtttg
    15721 gcgaagatgg cgggttcgat gcggtcgagt tggaatgcgg cgagtttgcg gttccactcg
    15781 attttgtggt tgctgccggc ggggatttcg ttgacgacgt tgatgatgcc gccgtccacg
    15841 tcgcccgggg tcaggatttg gttgaagtct gccatttggt ttcctttatt tgggaaggtt
    15901 tgaagtttga aagtatagca caaacgtccg gctggaaatg cgtccgatgc cgtctgaaag
    15961 ggaaaccggg cgcgtgttac cgtttgccca aaaacctgcc cagttccaaa atcgcgcgcc
    16021 tgttggacgg ggaaaacact ttttccgccg cttcttccaa gccgaaccag ccgtaggaga
    16081 cgtgttcctc gggttgcagg acgacgggcg tatcgcgcgg gatttcggcc cggaagacgt
    16141 gttcgcggtt ttcaaacacg cctttggggt agcggtgccg ccagtggtgg tagatttcgt
    16201 aaaccgtgct gtcgtgccgg tcttggagct gcccgtcctc cagcaggatg ccggtttctt
    16261 cccaaacttc gcgccttgcc gtttgggcga cggtttcgcc cggttcgagg ctgccggtta
    16321 ccgactgcca aaatcctttc ggatgcgtgc gttcgatgag caggatgccg ccgtccccgc
    16381 tataaaggac gaccagtgcg gaaacggggt atttgagcgg ttttgccatc ggcatctttc
    16441 ggcgggctgc ggtaatgaag gggcggatta tagcaaacgc cgcacgttat ggcgtttatc
    16501 cttttccgta tccttttttc tgcacggatg ggacgcgccg gtgtttgccg gtaaattttc
    16561 ctttgtgtca aaaagataag ggcggttgtg attttaatgc ttgccaaagt gacgggcgga
    16621 aactataatc cgcgacttac cgagtcggag tgtggcgcag tctggtagcg cacttgcatg
    16681 gggtgcaagg ggtcgaaggt tcgaatcctt tcactccgac caaaaattcc gaaagccgct
    16741 ttcaaaagcg gcttttttgc cgtccgtatg attatgatgt agagtacgcg gcgacagaca
    16801 ttcaaatgcc gtccgaaaac cgttcagacg gcatctcttt atcttaatag tggattaaca
//
LOCUS       DAXDXW010000052        16774 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXDXW010000052 DAXDXW010000000
VERSION     DAXDXW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16774
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.053"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            153..1217
                     /locus_tag="ACFB64_001426"
     CDS             153..1217
                     /locus_tag="ACFB64_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC1209771.1"
                     /translation="MKAARFYNKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPGANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDKLVSEG
                     FERLIHNNESAVKIIVNPNL"
     gene            1358..1681
                     /locus_tag="ACFB64_001427"
     CDS             1358..1681
                     /locus_tag="ACFB64_001427"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC1209772.1"
                     /translation="MGDSVLSAIQQTIIQRKSADPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKDPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELSRRQGLSGLAEKA
                     ARTES"
     gene            1755..2078
                     /locus_tag="ACFB64_001428"
     CDS             1755..2078
                     /locus_tag="ACFB64_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC1209773.1"
                     /translation="MDNCIFCKIAAKEIPAQTVYEDGEMVCFKDINPAAPLHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKASGLTDGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2125..2328
                     /gene="tatA"
                     /locus_tag="ACFB64_001429"
     CDS             2125..2328
                     /gene="tatA"
                     /locus_tag="ACFB64_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC1209774.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2332..3018
                     /gene="tatB"
                     /locus_tag="ACFB64_001430"
     CDS             2332..3018
                     /gene="tatB"
                     /locus_tag="ACFB64_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC1209775.1"
                     /translation="MFDFGLGELIFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQELDTQIELEELRKVKQAFEAAAAQVRDSLKETDTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDTANTVSDGISDVMPSERSDTSAETLGDDRQTGSTAEPAE
                     TDKDRAWREYLTASAAAPVVQAVEVSYIDTAVETPVPHTTSLRKQAINRKRDFRPKHR
                     AKPKLRVRKS"
     gene            3031..3801
                     /gene="tatC"
                     /locus_tag="ACFB64_001431"
     CDS             3031..3801
                     /gene="tatC"
                     /locus_tag="ACFB64_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC1209776.1"
                     /translation="MSETQNEQTVQPLVEHLIELRRRLMWMVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLVSLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTAFEVPIVVILLTKIGAVTTEQLKHARPYVIVGAFVVAAVITPPDIIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPAKT"
     gene            3845..4453
                     /locus_tag="ACFB64_001432"
     CDS             3845..4453
                     /locus_tag="ACFB64_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC1209777.1"
                     /translation="MNTLYLGSGSPRRMEILTQLGYRVVKLPAGIDETVKAGETPAPY
                     VQRMAEEKNQAALTLFCETNGAMPDFPLITADTCVFSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMEKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRPPLSALKP"
     gene            4462..4815
                     /locus_tag="ACFB64_001433"
     CDS             4462..4815
                     /locus_tag="ACFB64_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC1209778.1"
                     /translation="MNRQTAYFLASFSLIALIALSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFNAYPAEKICAALSAVFSIIFFIS
                     CLSFVKQYKETNNVR"
     gene            4805..6733
                     /locus_tag="ACFB64_001434"
     CDS             4805..6733
                     /locus_tag="ACFB64_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFC1209779.1"
                     /translation="MSAETTVSGARPAAKLPIYILPCFLWIGIIPFTFALRLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWWLQARLMNLIYPGMNDI
                     ASWVFILLAVSAWACKSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWENTPL
                     LQNIIVHRGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNAILETF
                     TGIRYETAVERVANGGFTDLPRQSEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     TIHDNFLSTLFTHSHNIILQLLAEMGISGTLLVAATLLTGIAGLLKRSLTPASLFLLC
                     ALAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNSFSPAADDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKALKYRPYSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPETKPCKRPASADADTARNVKLHARHC
                     KKQQTGREIRYQKTLQPC"
     gene            6809..7486
                     /gene="misR"
                     /locus_tag="ACFB64_001435"
     CDS             6809..7486
                     /gene="misR"
                     /locus_tag="ACFB64_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC1209780.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7499..8905
                     /gene="misS"
                     /locus_tag="ACFB64_001436"
     CDS             7499..8905
                     /gene="misS"
                     /locus_tag="ACFB64_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC1209781.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFKTRGDNGAREILTEWKNSPVSSAVYVIQGDEKKDILNRYIDNYTIE
                     RARLFAANNPHSNLVRIEYDRFGEEYLFFIKGWDNHQAQRLPSPLFIPGLPLAPIWHE
                     FIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMAEKLEKLVAKERYLLHHVSHEMRSPLARMQAIVGLIQAQPQKREQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVAL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGFKTEKSAN"
     gene            9023..11446
                     /locus_tag="ACFB64_001437"
                     /pseudo
     CDS             9023..11446
                     /locus_tag="ACFB64_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            11491..11736
                     /gene="rpsP"
                     /locus_tag="ACFB64_001438"
     CDS             11491..11736
                     /gene="rpsP"
                     /locus_tag="ACFB64_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC1209782.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAV"
     gene            11752..12261
                     /gene="rimM"
                     /locus_tag="ACFB64_001439"
     CDS             11752..12261
                     /gene="rimM"
                     /locus_tag="ACFB64_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC1209783.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLA
                     KDGKTVSVTLEAGKVVNGELQVKFEGIDDRDSAFSLRGYTIEIPREAFAPTEEDEYYW
                     ADLVGMTVVNKDDTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12261..13010
                     /gene="trmD"
                     /locus_tag="ACFB64_001440"
     CDS             12261..13010
                     /gene="trmD"
                     /locus_tag="ACFB64_001440"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC1209784.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKPLTHQKAAEL
                     AELTHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGIL
                     GDIQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLNKILQEQREIQS"
     gene            13025..13390
                     /gene="rplS"
                     /locus_tag="ACFB64_001441"
     CDS             13025..13390
                     /gene="rplS"
                     /locus_tag="ACFB64_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC1209785.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13711..14436
                     /locus_tag="ACFB64_001442"
     CDS             13711..14436
                     /locus_tag="ACFB64_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC1209786.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTSQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TESYLAQASAKMQQQESPDWCAS"
     gene            14471..15346
                     /locus_tag="ACFB64_001443"
     CDS             14471..15346
                     /locus_tag="ACFB64_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC1209787.1"
                     /translation="MNLYDLLLAPFAEFDFMHYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGVGGFAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVILISKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSGLTLITLAVIY
                     RPLVLESIDPLFLKSVNGKGGLWHVVFLILVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWARNMGTLILLSVLIALFCGLIGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILPKWFKNHRHHTT"
     gene            15423..16337
                     /locus_tag="ACFB64_001444"
     CDS             15423..16337
                     /locus_tag="ACFB64_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFC1209788.1"
                     /translation="MKHLKLTLITALLATAATAAPLPVVTSFSILGDVAKQIGGERVA
                     VQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSDYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGNRYNISFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNVKALTNAMKQ"
     gene            16557..>16774
                     /locus_tag="ACFB64_001445"
     CDS             16557..>16774
                     /locus_tag="ACFB64_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC1209789.1"
                     /translation="MKNILLVFVSFVPLCVRTDLPLNIEDIMTDKGKWKLETSLTYLN
                     SENSRAALASPVYIQTGSASFIPVPTEI"
BASE COUNT         4375 a         5340 c         3680 g         3379 t
ORIGIN      
        1 gagggaaggg aattttgcaa aggtctcagt ttgtagtaaa atatctaaac agattcaatc
       61 ggaaacggtc aggcttcttc acgccattcc catttcaacc gccgcctgct agattcagac
      121 ggcattccta cttgaaaaca aggagcgtta caatgaaagc agcacgtttt tacaacaaag
      181 gcgacatccg catcgaagac atccccgaac cgaccgtcgc ccccggcacc gtcggcatca
      241 atgtcgcctg gtgcggcatc tgcggtaccg acctgcacga atttatggaa ggcccgattt
      301 tcatcccgcc ctgcggtcat ccgcatccga tttccggcga gtccgcgccc gtaacgatgg
      361 gacacgagtt ctccggcgtg gtttatgccg tcggcgaagg cgtggacgac attaaagtcg
      421 gccaacacgt cgtggtcgaa ccctacatca tccgcgacga cgtaccgacc ggcgaaggca
      481 gcaactacca cctctccaaa gacatgaact ttatcggctt gggcggctgc ggcggcggcc
      541 tgtccgaaaa aatcgccgtc aaacgccgtt gggtgcatcc gatttccgac aaaatcccgt
      601 tggatcaagc tgctttgatt gaacctctgt ctgtcggaca ccacgcctat gtacgcagcg
      661 gcgcgaaagc aggcgatgtc gcattggtcg gcggcgcagg cccgatcggt ttgctgttgg
      721 ccgccgtgtt gaaagccaaa ggcatcaaag tcatcattac cgagttgagt aaagcacgca
      781 aagacaaagc ccgcgaatcc ggcgttgccg actacatcct cgacccgtcc gaagtcgatg
      841 ttgttgaaga agtgaaaaaa ctgaccaacg gcgaaggcgt ggacgtggcg ttcgaatgta
      901 ccagcgtgaa caaagtgttg gatactttgg tcgaagcctg caaacccggc gccaacttgg
      961 ttatcgtatc catctggagc caccccgcca ccgtcaacgt ccacagcgtc gtgatgaaag
     1021 aactggatgt gcgcggcacg attgcctact gcaacgacca cgcggaaacc atcaaactgg
     1081 tcgaagaagg caaaatcaac ctcgaaccct tcatcaccca gcgcatcaag ctggacaaac
     1141 tggtttccga aggcttcgag cgtctgatcc acaacaacga atccgccgtt aaaattattg
     1201 tgaatccaaa cctgtaagca aattacattt gcaacagcac aaatgccgtc tgaaacgctc
     1261 agacggcatt ttccaacgaa acgcaacagg cgtattgccc gagagcagcc gtttagccta
     1321 tcattccgaa caaacagaaa caaacagaag gaaaatcatg ggagattccg tactatccgc
     1381 catccaacaa accattatac agcgcaaatc tgccgacccg tccgaatctt acgtcgcaca
     1441 gctcctgcac aagggcgagg acaaaatcct aaaaaaagtg attgaagaag cgggcgaagt
     1501 gttgatggca tccaaagaca aagacccgtc ccacctggtt tacgaagttg ccgacttatg
     1561 gttccacacc atgattcttc tgacacacca cgacctgaag gcggaagacg tattggacga
     1621 actttcccgc cgtcaggggc tgtcggggct ggccgaaaaa gccgcccgta cagaatcctg
     1681 aatttatatt acaatctcat cccttccacg tttaatccgt ctgcgtgctt ttttcagacg
     1741 gcaacggagc cgttatggac aactgtattt tctgcaaaat cgccgccaaa gagattccgg
     1801 cgcaaaccgt ctatgaagac ggcgaaatgg tttgtttcaa agacatcaac cccgccgctc
     1861 cgcttcatct gctgctgatt cccaaagtcc attttgattc gttggcacac gccgcgcccg
     1921 aacatcagcc ccttttggga aaaatgatgc tgaaagttcc cgaaatcgcc aaagcttcgg
     1981 gactgaccga cggtttcaaa acccttatca ataccggcaa aggcggcgga caagaggtct
     2041 tccacctgca tatacacatc atgggcacac ccgtataaac cgttatttca caatcaaccc
     2101 ctaatactta cttaaggata catcatgggc agtttttctc tgacgcactg gattatcgta
     2161 ctgattatcg tcgttttgat attcggcacc aaaaaattgc gcaacgtcgg caaagacctc
     2221 ggcggtgcgg ttcatgactt caaacagggg ctgaacgaag gcacagacgg caaagaagcc
     2281 caaaaagacg atgtaatcga acacaaaaaa gacgaagaca aagcataact tatgtttgat
     2341 ttcggtttgg gcgagctgat ttttgtcggc attatcgccc tgattgtcct tggtccagaa
     2401 cgcctgcccg aagccgcccg cactgccgga cggcttatcg gcaggctgca acgctttgta
     2461 ggaagcgtca aacaagaact tgacactcaa atcgaactgg aagagctgag gaaggtcaag
     2521 caggcattcg aagctgccgc cgctcaggtt cgagacagcc tcaaagaaac cgatacggat
     2581 atgcagaaca gtctgcacga catttccgac ggtctgaagc cttgggaaaa actgcccgaa
     2641 cagcgcacgc ctgccgattt cggtgtcgat gaaaacggca atccccttcc cgatacggca
     2701 aacaccgtat cagacggcat ttccgacgtt atgccgtctg aacgttccga tacttccgcc
     2761 gaaacccttg gggacgacag gcaaaccggc agtacagccg aacctgcgga aaccgacaaa
     2821 gaccgcgcat ggcgggaata cctgactgct tctgccgccg cacctgtcgt acaggccgtc
     2881 gaagtcagct atatcgatac tgctgttgaa acgcctgttc cgcacaccac ttccctgcgc
     2941 aaacaggcaa taaaccgcaa acgcgatttt cgtccgaaac accgcgccaa accgaaattg
     3001 cgcgtccgta aatcataaag ggggcatccg gtgtccgaaa cacaaaacga acaaaccgtc
     3061 caaccgcttg tcgaacacct catcgagcta cgccgccgcc tgatgtggat ggttgtcggc
     3121 attctcgtct gctttttcgg catgatgccg tttgcccagc agctctatac ttttatcgcc
     3181 gacccgctga tggcaaacct gcccaaagac accagtatga ttgcgaccga tgtcatcgca
     3241 ccgtttttcg ttccggtcaa agttaccctg atggcggcgt ttttggtttc actgccgcat
     3301 acgctctacc aaatctgggc attcgtcgcc cccgcactct accaaaacga aaaacgcctg
     3361 attacgccgc tcgtcctctc cagcgtcagc ctgtttttca tcggcatggc atttgcctat
     3421 ttcctggttt tccccgtcat tttcaaattc cttgccggcg taacccccgt cggggtcaat
     3481 atggcgaccg acatagacaa atacctgtcc tttatcttag gtatgttcgt tgcgttcggc
     3541 acggcatttg aagtgcccat cgtcgtcatc ctgttaacca aaatcggcgc agtcacgacc
     3601 gagcagctca aacacgcccg gccttatgtc attgtcggcg cgtttgtcgt tgccgcagtc
     3661 atcacgccgc ccgacattat ttctcagacc ctgcttgcca ttccgctgat cctcttatac
     3721 gaggcgggca tttggttcgg acgcttcttc actccacgtt cagaacagga tggcgacata
     3781 cagccgcctg caaaaacctg acactatgcc gtctgaagcc ccgccgcaca ttaaggagca
     3841 caccgtgaac accctttact taggttcggg cagcccgcgc cggatggaaa tcctgacaca
     3901 gttgggctat cgggtcgtca agctgcccgc cggcatcgac gaaaccgtta aagccggcga
     3961 aacgcccgcc ccttacgtcc aaaggatggc ggaagaaaaa aatcaggcgg ctttgaccct
     4021 cttttgcgaa accaacggcg caatgcccga tttccccctg attaccgccg acacctgcgt
     4081 cttttcagac ggcatcatat tgggcaaacc tcgctcccaa gccgaagcaa tcgaattttt
     4141 aaaccgattg tccggcaaac aacataccgt cctgactgct gtctgcattc attatcgcgg
     4201 caaaacgtca agccgcgtcc aaaccaaccg cgtcgttttc aagcccctga gttcggaaga
     4261 aatttccgcc tatgtgcaaa gcggcgaacc gatggagaaa gccggtgcct acgccgtgca
     4321 aggcataggc ggtatcttta tccaatctat cgaaggcagc ttcagcggca ttatggggct
     4381 gcccgtttat gaaaccgttt ccatgttgca ggatttggga taccgccccc ccttatccgc
     4441 ccttaaaccg taaagtccgc cgtgaacaga caaaccgctt acttccttgc ctccttcagc
     4501 ctgattgcac tgattgccct atccctttct tgggaattat ggattgcacc gttacgcccg
     4561 ggcggttcgt ggctcgcgct caaagccctg cccctctgcc tgccgctttc aggtattcta
     4621 aagaaaaaaa tctataccta ccaatacagc tccatgctgg ttctgattta ctttgccgaa
     4681 gccgtcatgc gcctgttcaa cgcctatccc gcagaaaaaa tttgcgccgc gctttccgca
     4741 gtattcagca tcatcttctt catatcctgc ctgtcgttcg tcaaacaata caaggaaaca
     4801 aacaatgtcc gctgaaacga ccgtatccgg cgcgcgcccc gccgccaaac tgccgattta
     4861 catcctgccc tgcttccttt ggataggcat catccccttt accttcgcac tcaggctgaa
     4921 accgtcgccc gacttttacc acgatgccgc cgccgcggcc ggcctgattg tcctgttgtt
     4981 cctcacggca ggaaaaaagc tgtttgatgt caaaatcccc gccatcagct tcctcctgtt
     5041 tgcaatggcg gcattttggt ggcttcaggc acgcctgatg aacctgattt atcccggaat
     5101 gaacgacatc gcctcttggg ttttcatctt gctcgccgtc agcgcgtggg cctgcaagag
     5161 tttggtcgca cactacggac aagaacgcat cgttaccctg tttgcctggt cgctgcttat
     5221 cggctccctg cttcaatcct gcatcgtcgt catccagttt gccggctggg aaaacacccc
     5281 cctgcttcaa aacatcatcg ttcacagagg gcaaggcgta atcggacaca tcgggcagcg
     5341 caacaacctc ggacactacc tcatgtgggg catactcgcc tccgcctacc tcaacggaca
     5401 acgaaaaatc cccgcagccc tcggcgcaat ctgcctgatt atgcagaccg ccgttttagg
     5461 tttggtcaat tcgcgcacca tcttgaccta catagccgcc atcgccctca tccttccctt
     5521 ctggtatttc cgttcggaca aatccaacag acggacgatg ctcggcatag ccgcagccgt
     5581 attccttacc gcgctgttcc aattttccat gaacgccatt ctggaaacct ttacaggcat
     5641 ccgctacgaa actgccgtcg aacgcgtcgc caacggcggt ttcacagact tgccgcgcca
     5701 aagcgaatgg aataaagccc ttgccgcctt ccagtccgcc ccgatattcg ggcacggctg
     5761 gaacagtttt gcccaacaaa ccttcctgat caatgccgaa cagcacacca tacacgacaa
     5821 cttcctcagc accttgttca cccattccca caacatcatc ctccaactcc ttgcagaaat
     5881 ggggatcagc ggcacgcttc tggttgccgc aaccctgctg acgggcattg ccgggctgct
     5941 gaaacgctcc ctgacccccg catcactttt cctgctgtgc gcgcttgccg tcagtatgtg
     6001 ccacagtatg ctcgaatatc ctttgtggta tgtctatttc ctcatcccct tcggactgat
     6061 gctctttctg tcccccgcag aggcttcaga cggcatcgcc ttcaaaaaag ccgccaatct
     6121 cggcatactg accgcctccg ccgccatatt cgcaggattg ctgcacttgg actggacata
     6181 cacccggctg gttaactcct tttcccccgc cgctgacgac agtgccaaaa ccctcaaccg
     6241 gaaaatcaac gaactgcgct atatttccgc aaacagcccg atgctgtcct tttatgccga
     6301 cttctccctc gtaaacttcg ccctgccgga ataccccgaa acccagactt gggcggaaga
     6361 agcaaccctc aaagcactaa aataccgccc ctactccgcc acctaccgca tcgccctcta
     6421 cttgatgcgg caaggcaaag ttgcagaagc aaaacaatgg atgcgggcaa cacagtccta
     6481 ttacccctac ctgatgcccc gatacgccga cgaaatccgc aaactgcccg tatgggcacc
     6541 gctgctgccc gaactgctca aagactgcaa agccttcgcc gccgctcccg gccatccgga
     6601 aacaaaaccc tgcaaacgac ccgcgtcggc ggatgcggat accgcccgaa atgtaaagct
     6661 ccatgcaaga cattgcaaaa aacagcaaac cggtagggaa atacgctatc aaaaaacatt
     6721 gcagccgtgt taagataaac tgtcaaacaa tcttttcacg ccacgcccga aacagggtcg
     6781 gggcataccc ttacgaaaag gaaacaccat gagccgcgta ttactcgtag atgacgatgc
     6841 cctgctgacc gaactgctga ccgaatacct gagcgccgaa ggtctgaacg tccgcagcgt
     6901 tcccgacggg gaagcaggcg tacaggaaat cctgagcggg caatacgatg tagtcgtatt
     6961 ggattccatg atgcccaaaa tgaacggctt ggatgtcttg aaaaacgtac gcgcccgaag
     7021 caccgtcccc atcatcatgc tgaccgccaa aggcgacgac atcgaccgaa tcatcggctt
     7081 ggaaatgggt gcggacgact atgtccccaa accctgcaca ccacgcgaac tcttggcacg
     7141 catcaatgcc atcctccgcc gagcacaaca cagcggcgaa cagaacaacg cacccaacag
     7201 catctccgtc agcgatgtcg tcctataccc cgccaaacgc caggcatccg tcaaagacat
     7261 gccgctcgaa ctgaccagca ccgaattcaa cctgctcgaa gtcctgatgc gccatgccgg
     7321 acaggtagtc agcaaagaaa ccctgtccgt cgaagcactc gaccgcaaac tggcaaaatt
     7381 cgaccgcagt atcgacgtac acatctccag catccgccac aagctgggcg atgcctccct
     7441 gattcaaacc gtacgcggct tgggctacct gtttgtcaaa aactgaagca gcgggcaaat
     7501 gaaactgttc caacgcattt tcgccacatt ttgcgcggtt atcgtctgcg caatctttgt
     7561 ggcgagtttt tctttttggc tggtgcagaa cacccttgcc gaaaaccaat tcaaccaacg
     7621 ccgcaccatc gaaaccacat tgatgggcag cattatttcc gcattcaaga cacggggcga
     7681 caacggcgcg cgcgaaatcc tgaccgaatg gaaaaacagc cccgtctcat ccgccgttta
     7741 cgtcatacag ggcgacgaga aaaaagacat cttaaaccgc tatatcgaca attacaccat
     7801 agaacgcgcc cggttgtttg ccgccaacaa cccccattcc aaccttgtcc gcatcgaata
     7861 cgaccgtttc ggcgaagaat acctgttctt cattaaaggc tgggacaacc accaggcaca
     7921 acgcctgccc agcccgctgt ttatcccggg cctgccgctt gccccgattt ggcacgaatt
     7981 catcatcctc tccttcatca tcattgtcgg actgctgatg gcatatatcc ttgccggcaa
     8041 cattgccaaa cccatcagaa tcttaggcaa cggcatggac agggtggcaa acggagaact
     8101 tgaaacccgc atctcccaac aggtcgacga ccgcgacgac gaattgtccc atcttgccat
     8161 ccaattcgac aaaatggcag aaaaactcga aaaacttgtc gccaaagaac gttacctact
     8221 ccaccacgtt tcccacgaaa tgcgctcccc gcttgcccgt atgcaggcaa ttgtcggact
     8281 gattcaggca cagccccaaa aacgggagca atatctcaaa cggctggaag gcgaactgac
     8341 ccgcatggat acgctggccg gggaactgtt aaccctgtcc cgcctcgaaa cctccaatat
     8401 ggctttggaa aaagaaagcc tgaaactcct gcccttcctg ggcaacctgg tagaagacaa
     8461 ccaaagtatc gcccaaaaga acggacagac ggtcgccctg tctgccgacg gaaaaatccc
     8521 cgaaaacaca accatccttg ccaacgaaag ctacctgtac cgcgccttcg acaacgtcat
     8581 ccgcaacgcc gtcaactaca gtcccgaagg cagcaccatc ctgatcaaca tcggacaaga
     8641 ccacaaacac tggataatcg acgttaccga caacggcccc ggcgtggacg aaatgcagct
     8701 cccgcacatc ttcaccgctt tctaccgtgc agactccagc gccaacaaac ccggaacagg
     8761 actggggctt gcattgaccc aacatattat tgaacagcac tgcggcaaaa tcatcgccga
     8821 aaacatcaaa ccgaacggtc tgcggatgcg ctttatcctg cccaagaaaa aaaccggttt
     8881 caaaacagaa aaaagtgcga actgaccata atgccgtctg aacccgcttc agacggcatt
     8941 gcacaaacag ttaccccaaa acaaacacac agcgggtgca tctccaaaaa tcacgcacat
     9001 accgtccgca aaggaatata tcatgccggc acaaaccgat ccgggctact tcttcatgcc
     9061 caaccacatt atcctgatag gcgcgagcga acaaccgtac agcctgggtg aacgcgtact
     9121 cagcaacctg ctgagtacgc cctttcaagg aaaaatcacc cccgtaaacc cgcgccacca
     9181 caccatagcc gggcttcccg cctacaccag cctcaacaaa atccccggca gtgcagacct
     9241 gattattacc gttaccccgc ccgacagtta cgacaccctc ctcaaaacct gccgtaaaaa
     9301 gcagctccga cacatcatcc tcatacagga ctgggacagc ctgtctgccg cagaactgca
     9361 caccgccgaa actgccatcc gcaaacacca cggcaacgga ctcaacatca ccgcctacac
     9421 caccgcaggc atccaactgc cctcactcgg actcaacatc agtacccaag acggatatgc
     9481 cgcaggccat accgccatac tgaccggcaa tgccgccgtc agccgccaaa tcgacaacat
     9541 cctgaacaaa ctccgtcaag gcacatcccg ccacatcaac ctgcatcccg gcatcagccc
     9601 catcacatcc gccgattggc tcaaccgctt cggacacagc ctgcacacca aaaccgccgt
     9661 cctacaccac aaccctgaag aggatcagcg caggctgttc agcgcaatcc gccaatttac
     9721 ccgccatacg ccgctgattc tccacatcac ctgcctcacg acagaaaccg accgtgccgt
     9781 actgcactgc ctcgcccgac actgcaactt cctcatcagt ttcaacgccg acgacctcga
     9841 agccgcactg agcgcccaac tgtccgacct tccacccctg tcccgactcg acatcctgtc
     9901 cgacacgcct gccgaatggc tgcacgcgca cgcaccaaaa aacctcaccc tccactttcc
     9961 caaccttccc caccacatcc gcaacggaca cctgaccggc acacccacac cctcaatctg
    10021 ccacgacatc gcctcacgtc agcttgcccg ccccgacacc caagccgtcc tgaccatcct
    10081 cggtccctcc ggacacgagg attacaaaaa aacagcacgc gcccttatcc gcctgtccga
    10141 acagaccgcc aaacccctgc tcgtcaacag ccccttttca gacggcataa cacatttcga
    10201 cacccccgca caggcaatcc gcaccctttc ctaccgtaac accgccgccg ccctgaaaca
    10261 ggcacagctc gacattgcac cgccgcagcc atgccgtctg aaaacaccgc aaccccaaaa
    10321 catcaaaaaa gcccttgcag cggcaaatcc cgccctgctc gccgaagccc tgcacctccc
    10381 cctaccggca caccacccat aacgccgtcc aattccaatt cggcagccac cccctctatg
    10441 gcgacatcct gaccgcacgc tgcaacggac aaaccactgc cgtactcccg ccgtttacca
    10501 cgctcgacag ccgccacctt gcccgctttg ccgaactcga cggcacacaa accctcgatc
    10561 agttcctgca cacactgacc gtcatccccg aataccgcca acacattttc ggcatcaccc
    10621 tcaacctcaa cggcggacaa tacagcagcg atttcatctt aaagacaccc ggaacacacg
    10681 acacgcccaa acgcaagaac acaggcaaag ccgcccaaac cctcgaacat gccgccgcaa
    10741 aaatgcagag tgccgccgca tacctggaac acaaaaaccc gacagccgcc gaatttctcc
    10801 gccacacaag cgaagccgcc gcagaactgc tcggcagcaa aaccgaaacc ggagcagccg
    10861 tacccaacgt acttgccccc tatcccgcag cacaccccaa aacactgtcc ctaaaaaaca
    10921 acacgaccgt taccattatc ccccttttac ccgaagacgc agaagccaaa cagcagttcg
    10981 tccgcagcct cggccccgaa gcacggtaca cacgcttcat gacccacacc aacgaactgc
    11041 ccgcagccac gttggcacgc ctgtgcaacc ccgattacca ctgtgaaccg catggacggc
    11101 aagggatgcc gacagcaaca tcgtcgccgt cgtccgccac agccgcctga accgcaacga
    11161 atgcgaattt ggcatcacac tggcggaaca tatgcgcggc agcgggctgg cacagaaaat
    11221 gatggaactc atcatccaaa ccgccgcaca gcaaggctac ctgagcatga gtgccgacat
    11281 tctcaaaacc aataccccca tgatcaaact tgctgaaaaa tcaggattta ccctcaagga
    11341 atcggacacc gaaaaaaacc tgtaccgcgc atatctgaac cttgcggcag acaaaacaac
    11401 aggaaaaaca aataaaaact tgcgcaccgg ccacaaaata acctaaaatc ggcagtttcc
    11461 atatatccga ttttctcaaa aggactcaaa atggtagtta tccgtttggc acgcggcggc
    11521 tcgaaacacc gccccttcta caacgtcatc gttactgact cacgcagccg ccgcgacggc
    11581 cgcttcatcg aacgcgtagg cttctacaac cccgtagcca acgaaaaaca agagcgcgtc
    11641 cgcctcaatg cagaccgcct gaaccactgg attgcacaag gcgcgcaagt cagcgactcc
    11701 gttgcaaaac tgattaaaga acaaaaagcc gtctaatccg cgtttgccgc catgacagac
    11761 actcaaaacc gggtagccat gggctacatc aaaggcgtat tcggcataaa aggctggctg
    11821 aaaattgccg ccaacaccga atattccgac agccttttgg actaccccga gtggcatttg
    11881 gccaaggacg gcaaaaccgt cagcgttacc cttgaagccg gaaaagtcgt caacggcgaa
    11941 ctccaagtca aattcgaagg catagacgac cgcgattcag cattctcatt gcgcggttac
    12001 accatcgaaa taccccgtga agcattcgcc ccgacagaag aagacgaata ctactgggca
    12061 gacttggtcg gcatgaccgt cgtcaacaaa gacgataccg ttttaggcaa ggtaagcaac
    12121 ctgatggaaa ccggcgcaaa cgacgtattg atgattgacg gagaacacgg gcagattctg
    12181 attccgttcg tttcccaata tatcgaaacc gtcgataccg gcagcaagac cattactgcc
    12241 gactggggtt tggactactg atgcttatcc aggcagttac cattttcccc gaaatgttcg
    12301 acagcattac ccgctacggc gtaacgggac gcgcgaacag acagggaatc tggcagtttg
    12361 aagcagtcaa tccccgaaag tttgccgaca acagattggg ctatatcgac gaccgcccgt
    12421 tcggcggcgg tccgggaatg attatgatgg ctccgccgct tcatgcggcg atagaacacg
    12481 ccaaagcaca atcttcccaa accgcaaaag tcatctacct cagcccccaa ggaaaaccgc
    12541 tgacacacca aaaagcggca gaactggcag aacttacgca tctgattctg ctgtgcggac
    12601 gctatgaggg aatagacgaa agactgctgc aaagcagcgt cgatgaagaa atcagcatcg
    12661 gagacttcgt cgtttccggc ggagagcttc ccgccatgat gctgatggat gcggtattga
    12721 ggctcgtacc cggcatattg ggcgacattc agtctgccga acaggattcg ttctcaagcg
    12781 gtattttgga ctgcccccac tacaccaaac ccttagaatt tcaaggcatg gctgttccgg
    12841 aagtattgcg ctccggaaat catggcttga tagcggaatg gcggttggaa caatcgctgc
    12901 gccgcacctt ggagcgcaga cccgatcttt tggaaaagcg cgttttaatc ccaaaggaat
    12961 cccgcctctt gaataaaatc ctacaagagc aacgggaaat ccaatcataa tttaggaaaa
    13021 aacaatgaac ctgattcaac agctcgagca agaagaaatt gcccgcctga acaaagaaat
    13081 ccccgaattc gcaccgggcg acaccgtagt cgtatccgta cgcgtcgtgg aaggtacccg
    13141 cagccgtctg caagcctacg aaggcgtggt tatcgcccgt cgcaaccgtg gtttgaacag
    13201 caacttcatc gtccgcaaaa tctccagcgg cgaaggtgtt gaacgtactt tccaactata
    13261 ttcccctact gttgagaaaa tcgaagtcaa acgccgtggc gacgtacgcc gtgccaaact
    13321 gtactacctg cgcggtctga ccggcaaagc tgcacgcatc aaagaaaaac tgcctgcacg
    13381 caaaggttga ttcaaaccgt tttcccccaa tgccgtctga acgttcagac ggcatttttt
    13441 attgccgcga tgccggcaag gcaacgatat gcctgaccct catccacctt aattaacaat
    13501 cactcccgcc tattcaagca gaatttacag actaaacgtt atacagtatc atatccgcca
    13561 tataacgata accgctttgc caacttccct ttcaaaactg caaatcatta tgacaccttc
    13621 cctcttactg tcaggattga ccttccgact cttcctcgcc ctgattgccg tattcctttt
    13681 atggggtgtt tacctttggg cggtatcggt atgagcatca tcgttgaaaa cctgacggtc
    13741 agctaccgcc gccgccccgc cgtacaccac gtcgacatta cttttgaaga acatagtatg
    13801 tgggcggttt tcggtcccaa cggcgcagga aaatctacct ttctcaaatc cttaatggga
    13861 ttgcagccta tcgatacggg cagcatccgg ctggacggat tgaccagtca aaacattgcc
    13921 tatctccccc aacaatccga catcgaccgt tcccagccta tgaccgtttt cgacttggcg
    13981 gcaatggggc tgtggtatga aatcggtttt ttcaaaggga taaataccgc tcaaaaacaa
    14041 cgcgttcacg aagccttgga gcgtgtcggt atgcggcagt ttgcccgccg ccaaatcgcc
    14101 cacctctcaa acggacaatt ccaacgcgtc cttttcgccc ggatgctggt tcaaaatgca
    14161 aaattcctgc ttttggacga acctttcaat gctgttgatg cacggacaac ctacgagctt
    14221 ctcgacgtgt tgcaaaaatg ccattgcggc ggacacgcta tcattgccgt tttgcacgat
    14281 tacgaacaag tccgtgccta ctttcccaat accctgctgc tcgcccgcga aaaaattgcg
    14341 gcaggcgcaa ccgagaccat tctgacagaa tcctacctcg cccaagccag cgccaaaatg
    14401 cagcaacagg aaagccccga ctggtgcgcc tcataaatac tgcccgaaac cacgccccac
    14461 accagacacc atgaacctct acgacctgct ccttgccccc tttgcagaat tcgacttcat
    14521 gcactacgcc ctcgcatccg tcttctgcct gtccctcagc gccgcacccg tcggcgtatt
    14581 cctcgtcatg cgccgtatga gcctgatagg cgacgcattg agccacgccg tcctgcccgg
    14641 tgccgccgtc ggctacatgt ttgccggctt gagcctgccc gctatgggtg tgggcgggtt
    14701 tgccgccggt atgctgatgg cgctgcttgc cggactcgtc agccgcttta ccaccctgaa
    14761 agaagatgcc aactttgccg ccttttacct gagcagcctc gccatcggcg taatcctcat
    14821 cagcaaaaac ggcagcagcg tcgatttact ccacctcctt ttcggatctg tgcttgccgt
    14881 cgatattccc gcactgcaac tcatcgccgc cgtctccggc ctcacgctca ttacccttgc
    14941 cgtcatctac cgccccctgg tgctagaaag catagacccc ctgttcctca agtccgtcaa
    15001 cggcaaaggc gggctttggc acgtcgtttt cctcatcctc gtcgttatga acctcgtatc
    15061 cggcttccaa gctctcggca ccctgatgtc ggtcggactt atgatgctgc ccgccattac
    15121 cgcccgctta tgggcaagaa atatggggac gctcattctg ttgtccgtcc tcatcgccct
    15181 tttttgcggt ttgatcgggc tgctcatttc ctaccacatc gaaatccctt ccggccccgc
    15241 catcatcctc tgttgcagcg tcctttatct cttttccgtc atactcggca aagaaggcgg
    15301 catcttgccc aaatggttca aaaaccaccg ccaccacacc acctgacccc gcaagccgct
    15361 tgaacaaaca ggcggcttgt tttaccataa tgaatgttat gttatatcac agaagaacag
    15421 ttatgaaaca cctcaaactc acccttatta ccgcattgct ggccaccgcc gcaactgccg
    15481 caccccttcc ggttgtaacc agtttcagca ttttaggcga cgtagccaaa caaatcggcg
    15541 gtgagcgcgt agccgtacaa agcctcgtcg gagccaacca agatactcat gcctatcaca
    15601 tgaccagtgg cgacattaaa aaaatccgca gtgcaaaact cgtcctgctc aacggcttgg
    15661 gacttgaagc cgccgacatc caacgcgccg tcaaacagag caaagtatcc tatgccgaag
    15721 cgaccaaagg catccaaccc ctcaaagccg aagaagaagg cggacaccat cacgaccacg
    15781 atcatgacca cgaaggacac caccacgacc acggcgaata tgacccccac gtctggaacg
    15841 accctgttct tatgtccgac tatgcccaaa acgtcgctga agccctgata aaggccgatc
    15901 ccgaaggcaa agtttattat caacaacgct tgggcaacta ccaaatgcag cttaaaaaac
    15961 tgcacagcga cgcacaagcc gcatttaatg ccgtccctgc cgccaaacgc aaagtcctga
    16021 ccgggcacga cgcattttcc tacatgggca accgctacaa catcagcttc atcgccccgc
    16081 aaggcgtgag cagcgaagcc gagccgtccg ccaaacaagt cgccgccatc atccggcaaa
    16141 tcaaacgcga aggcatcaaa gccgtattta ccgaaaatat caaagacacc cgcatggttg
    16201 accgcatcgc caaagaaacc ggcgtcaacg tcagcggcaa actgtattcc gacgcactcg
    16261 gcaacgcgcc cgcagacacc tacatcggca tgtaccgcca caacgtcaaa gccttgacca
    16321 acgcgatgaa gcaataaaac cgtttgattg gtttgaattt gaaaaggcaa aaggccgtct
    16381 gaaaattaaa tacgaggttt tcagacggcc ttctcttgtg caataaagca gtaaacaaat
    16441 attgataaga aaaaaacaat cagtattgaa aatatcgtta aaaatattat ttagacagat
    16501 tacatggaat ttcattaaca tcagtagact ttgatttcaa tcaagaaaac aatgtcatga
    16561 aaaatattct cttagtattt gttagctttg tgccattatg tgtccgcact gatctgccgc
    16621 tgaatatcga agacataatg accgacaagg gaaaatggaa actggaaact tcccttacct
    16681 acctgaacag cgaaaacagc cgcgccgcac ttgcctcacc ggtttacatt cagaccggct
    16741 ccgcttcctt tatccccgtc ccgaccgaaa ttca
//
LOCUS       DAXDXW010000053        16642 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXDXW010000053 DAXDXW010000000
VERSION     DAXDXW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16642
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.054"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(201..686)
                     /gene="purE"
                     /locus_tag="ACFB64_001446"
     CDS             complement(201..686)
                     /gene="purE"
                     /locus_tag="ACFB64_001446"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC1209790.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFEYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAISMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(734..1447)
                     /locus_tag="ACFB64_001447"
     CDS             complement(734..1447)
                     /locus_tag="ACFB64_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC1209791.1"
                     /translation="MKTKLPLFIIWLSVSASCASVLPVPEGSRTEMPTQENASDGIPY
                     PVPTLQDRLDYLEGKIVRLSNEVEMLNGKVKALEHAKTHPSGRTYVQKLDDRKLKEHY
                     LNTEGGSASAHTVETAQNLYNQALKHYQNGRFSAAAALLKGADGGDGGSIAQRSMYLL
                     LQSRARMGNCESVIEIGGRYANRFKDSPTAPEVIFKIGECQYRLQQKDIARATWRSLI
                     QTYPGSPAAKRAAAAVRKR"
     gene            complement(1447..2115)
                     /locus_tag="ACFB64_001448"
     CDS             complement(1447..2115)
                     /locus_tag="ACFB64_001448"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC1209792.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKAGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAAFDAPPSVKILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(2126..2311)
                     /locus_tag="ACFB64_001449"
     CDS             complement(2126..2311)
                     /locus_tag="ACFB64_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC1209793.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELAQERLKNRGLLWDVELNPEEQK
                     NFESAKVAKKPYTYYQD"
     gene            2455..4431
                     /gene="mutL"
                     /locus_tag="ACFB64_001450"
     CDS             2455..4431
                     /gene="mutL"
                     /locus_tag="ACFB64_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC1209794.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAVD
                     VELEGGGIRLIRVGDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNSALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLELPPKAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRANLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDSASNHPTGNKPDTRNAFGSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMDTYAELYKKTDDIDLELSQFEQARFGNMPSETPAHKT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQHLLIPVTFAASHEECAALADHAETLAGFGLELSDMGGNTLAVRAAPVML
                     GKSDVVSLARDVLGELAQVGSSQTIASHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDTLFLRGQ"
     gene            4526..6649
                     /gene="dnaX"
                     /locus_tag="ACFB64_001451"
     CDS             4526..6649
                     /gene="dnaX"
                     /locus_tag="ACFB64_001451"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC1209795.1"
                     /translation="MTYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYDPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIVNQDGEALLAKAQEMAACAV
                     GFDNALGELAILLQQLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPEADAAQTPVQTASAAAMPSEGKTAGPVSHQENNDVPPWEDAPDKTETAAGTA
                     RTSAKSIQTASEAETPPENQVSKNKAADNETEASLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGFPDNDCPPEDGVEIPPPDWANVLPADTAGGGTDEEAEAGGIGGNNTP
                     SAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYRSDTGLMVLAMTAEARATAD
                     KKRLDKIRDTLAQAYGLQLTLQTEDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADP
                     AAQKILQAFGAQWQPESLELAANRP"
     misc_feature    5715..5754
                     /gene="dnaX"
                     /locus_tag="ACFB64_001451"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            6730..7065
                     /locus_tag="ACFB64_001452"
     CDS             6730..7065
                     /locus_tag="ACFB64_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC1209796.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQDL
                     PPGVGDFFR"
     gene            complement(7718..8764)
                     /gene="recA"
                     /locus_tag="ACFB64_001453"
     CDS             complement(7718..8764)
                     /gene="recA"
                     /locus_tag="ACFB64_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC1209797.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEISDEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            8964..9728
                     /gene="aroD"
                     /locus_tag="ACFB64_001454"
     CDS             8964..9728
                     /gene="aroD"
                     /locus_tag="ACFB64_001454"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC1209798.1"
                     /translation="MHTSLTVKNTVIGSGRTKIAVPLVARDAADLSSVLSQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQAVRDALPDKPLLFTFRRHCEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSSEDVLTLLSATLEAKRLVAKPVITMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            9748..11763
                     /gene="rep"
                     /locus_tag="ACFB64_001455"
     CDS             9748..11763
                     /gene="rep"
                     /locus_tag="ACFB64_001455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC1209799.1"
                     /translation="MMKLNAQQLEAVRYLGGPLFVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNVWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFALMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTRYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNAYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGNYRAKAEIDEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVGEL
                     VSWFARKGERDGKNVIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRIDNLKKSGPAD"
     gene            complement(11833..12891)
                     /gene="dinB"
                     /locus_tag="ACFB64_001456"
     CDS             complement(11833..12891)
                     /gene="dinB"
                     /locus_tag="ACFB64_001456"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC1209800.1"
                     /translation="MSLRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATVKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     PDEAYLDVTRNFKNIPYAGEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGAGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDEHPVKAERERLQISTEITLPEDLPLGQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGRLVPKNQQQDLWA"
     gene            12990..13199
                     /locus_tag="ACFB64_001457"
     CDS             12990..13199
                     /locus_tag="ACFB64_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209801.1"
                     /translation="MKLDKNNGFYQAERNSNGNYDIVRKNYLKNITKIQSNILIYLDN
                     TSTGFKIQQRRKQIRAQISARQWRR"
     gene            13791..14570
                     /locus_tag="ACFB64_001458"
     CDS             13791..14570
                     /locus_tag="ACFB64_001458"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC1209802.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGKGFIWRAYSVVSAEYADTLEYFAVLIQDGPMSALFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKLTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            14779..15849
                     /locus_tag="ACFB64_001459"
     CDS             14779..15849
                     /locus_tag="ACFB64_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC1209803.1"
                     /translation="MAAHNKLTQKQIEAAKADGKQSKLADGGGLYLLLHPNGSKYWRM
                     RYRHGGCEKTLALGVYPAVSLKQARELARAARAQTAAGIDPVAEHHRTRPGSGRSLPE
                     IARAWYGSRQGQRADNTLEGDRRSLAYLTDYYKDNTDINDITTAGVSKFIEHLNRINI
                     PACARRTVQILAQIWDYAVQRGIITDERRNPATTARPLLNTSKPKPQPHIRPDELPDF
                     YRTLQTAHDLHPYARPLLLLAALTVPRPSALLSARWQDIDLTARLWHIPAADMKTKHP
                     FTVPLSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYD
                     GSTPANPNTPCTDSATSSPTSA"
     gene            15810..16058
                     /locus_tag="ACFB64_001460"
     CDS             15810..16058
                     /locus_tag="ACFB64_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209804.1"
                     /translation="MHGFRDLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHH
                     YLTADSYRLQERRELAEWYGRRHRQAYEAAAQNTCNKT"
     gene            16078..16341
                     /locus_tag="ACFB64_001461"
     CDS             16078..16341
                     /locus_tag="ACFB64_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209805.1"
                     /translation="MADILPSDKRRQPPKTKGNPINIIEQETKRTELRKIDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            16378..>16642
                     /locus_tag="ACFB64_001462"
     CDS             16378..>16642
                     /locus_tag="ACFB64_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209806.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFGGKPADEARFAAVLTETCRRAVKK"
BASE COUNT         4250 a         4933 c         4101 g         3358 t
ORIGIN      
        1 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttaa tccactatat atttcaaaaa
       61 ttgaaacccg cacagattgt taaaccccaa aacaggggcg ggcgaaattc ttccggcaat
      121 gggttcatgc aaacaaaatg ccatctgaac cccgaaaaca gggcttcaga cggcatttgt
      181 gtattcgcgg acggcgggtt ttaaatctgt tccaattcca tgtttaaaac ggtttgttcc
      241 tgtttggctc ggaagtctgc cagtttttgc gccagttcgg ggttttcgtt ggcgagcatg
      301 gagatggcga acaatgcggc atttgccgcg cctgcctcgc cgatggcgaa tgtggcgacg
      361 ggtacgcctt tgggcatttg tacaatcgat aaaagcgaat cttcgccgcg caggtatttg
      421 cttgggacgg gtacgcccaa aacggggacg gtggtcttgg cggcaaccat gccgggtaaa
      481 tgcgccgcgc cgcccgcacc cgcgatgatg gctttgatgc cgcgcgcccg tgcggtttcg
      541 gcgtattcaa acatcaaatc cggagtgcgg tgtgcggaaa cgacgcgcgc ctcatattct
      601 acgccgaact cttcaagaaa ctgcgctgcc tgccgcataa cgggccaatc gctgttgctg
      661 cccatgatga tgccgatttg tatcataaat ccttcttggt gcggatgggg taaaaagcgg
      721 aaaaatggaa aaactatcgt ttgcgtacgg ctgcggcggc gcgttttgcc gccgggctgc
      781 cgggataggt ctgtatcagg ctgcgccaag tcgcccttgc aatgtctttt tgctgaagcc
      841 tgtattggca ttcgccgatt ttgaatatga cttcgggcgc ggttgggctg tctttgaaac
      901 ggttggcgta acgccctccg atttcgatga cagattcaca gttccccata cgcgccctgc
      961 tttgcagcaa caggtacata ctgcgttgcg cgatgctgcc gccgtctccg ccgtccgccc
     1021 ccttcaacaa ggcggctgcg gcagaaaacc tgccgttttg atagtgtttg agtgcctgat
     1081 tgtagaggtt ttgtgcggtt tcgacggtat gtgcggatgc gctgccgcct tcggtattga
     1141 ggtaatgctc tttcaatttg cggtcgtcga gtttttggac gtatgtcctg ccggaagggt
     1201 gtgtttttgc gtgttccaat gctttgactt tcccgtttaa catttccact tcgttcgaca
     1261 gccggacgat tttgccttcc agatagtcca aacggtcttg cagagtggga acgggatagg
     1321 gaatgccgtc tgaagcattt tcctgtgtcg gcatttcggt tcggctgccc tccggaacgg
     1381 gtaaaacgga agcacaggag gcagacacag acagccaaat gataaaaagc ggtaatttgg
     1441 tcttcattat tttttcagaa gcagggtcaa accgtcgccg acgggcaggg tgatggggac
     1501 gatgcgcgta tcgttcggca ggttttgatt gaaatctttg agaattttga cactgggcgg
     1561 cgcatcaaaa gccgcttcgc gcatcaccct tccgttcaac aaaatattgt cgatggcgat
     1621 gatgccgcct tgccggacga gtttgaggca acgctcgaaa tattgcggcg tgggcggttt
     1681 gtctgcgtct atcagtgcca aatcgtagct tccggcttca ccctgtgcaa tcaaatcatc
     1741 caatgtcagc aatgcgggtt gcaggtgcag gctgatttta tgcgccacac cggcctcgtt
     1801 ccaaacctga cgcgccgtat cggtaaaggt tacattgatg tcgcaggcgg taatccgccc
     1861 gtgttcgggc agcgccaatg caagcgcggt gctgctgtat ccggtaaata cgccgatttc
     1921 cagatatttt tccgcgcgaa tcagctttgc cagccaaacc aaaaccgccg cctgttcgcg
     1981 cgcaatcgcc attttgccca tacggtgatg cccggccttc tcacgcagcc gcgtcaaaac
     2041 gggatgttcg ggttcgccga tggcattcaa atagttttgc aggtccggtg cgacattgga
     2101 cagatgggtc gtcatttcgg cgggttcagt cttggtaata ggtataaggt tttttcgcca
     2161 cttttgccga ttcaaagttt ttttgctctt cgggattgag ttcgacatcc cacaaaagcc
     2221 ctcgattttt caaacgttct tgcgccaatt caggtttttc ttcgatcagg cggttgagga
     2281 attgtgtggc ttcggattgg tagtggtaca tctttgcgct ccaattttac gggatacggc
     2341 gtgattatac tggtattttc caaacgggat aaacggcttt tatcaagaat acgggcagaa
     2401 agataagggg ttttattata gaataaggcg ttttttgcaa cggaagaccg ccttatgcca
     2461 cgcattgccg ccctgcccga ccacctcgtc aaccaaattg ccgccggcga agtggtcgaa
     2521 cgccctgcca acgccctgaa agaaatcgtt gaaaacagca tagacgcggg cgcaacggcg
     2581 gttgatgtgg aactggaagg cggcggcatc cgcctcatcc gcgtcggcga caacggcggc
     2641 ggcatccacc ccgacgacat cgaactcgcg ctccaccgcc acgccaccag caaaatcaaa
     2701 accctgaacg atttggaaca cgttgccagc atgggctttc gcggcgaagg cttggccagt
     2761 atcgcctccg tcagccgcct gaccctgacc agccgccaag aagacagttc gcatgcgacc
     2821 caagtcaaag ccgaagacgg caaactcagc agccccaccg ccgccgccca ccccgtcggc
     2881 accaccatcg aagccgccga actcttcttc aacacccccg cgcggcgcaa gttcctcaaa
     2941 tccgaaaaca ccgaatacgc tcactgcgcc accatgctcg aacgcctcgc gctggcgcat
     3001 ccgcacattg ccttctcact caaacgcgac ggcaaacaag tgttcaaact tcctgcccaa
     3061 agcctgcatg aacgtatcgc cgccattgtc ggcgatgatt ttcagacggc atctttggaa
     3121 attgacagcg gcaatagcgc gctgcggctc tatggcgcga ttgccaagcc gactttcgcc
     3181 aaaggcaaaa ccgataaaca atattgcttc gtcaaccatc gttttgtccg cgacaaagtc
     3241 atgctccatg ccgtcaagca ggcataccgc gacgtattgc acaacgccct cacccctgct
     3301 ttcgtccttt tcctcgagct gccgcccaaa gccgtggatg tcaacgtcca cccgaccaaa
     3361 accgaaatcc gcttccgcga cagccggcaa gtacaccaac tcgtgttcca cacgctcaac
     3421 aaagcccttg ccgacacacg cgccaacctg accgaaagcg tcagcaacgc aggcgaagtg
     3481 ttgcatgaca ttaccggcgt tacgcctgcc ccaatgccgt ctgaaaacga cagcgaaaat
     3541 ctgtttgata gcgcatccaa ccacccgaca ggcaacaaac ccgatacacg caatgccttt
     3601 ggttcatcag gtaaaaccgc gcccatgcct taccaggccg cgcgtgcgcc gcaacaacac
     3661 agcctgtccc tgcgcgaaag ccgcgcggca atggacacct atgccgaact ctacaaaaaa
     3721 accgacgaca tcgaccttga gttgagccaa ttcgaacagg cacgtttcgg caatatgccg
     3781 tctgaaacgc ctgctcacaa aacagatacg ccgctttcag acggcatccc gtcccaatcc
     3841 gaactgccgc cgctcggttt cgccattgcc caactattag gcatctacat tcttgcccaa
     3901 gccgaagaca gcctgttgct catcgatatg cacgccgctg ccgaacgcgt caactacgag
     3961 aaaatgaaac gccaacgtca ggaaaacggc aacctgcaaa gccagcacct gcttattccc
     4021 gtaacctttg ccgcgtccca cgaagaatgc gccgccctcg ccgatcatgc cgaaacgctg
     4081 gcaggcttcg gcctggaact gtccgacatg ggcggcaaca ccctcgccgt ccgcgccgcg
     4141 cccgtaatgt tgggcaaatc cgatgtcgtc tccctcgcac gcgacgtatt gggcgaactc
     4201 gcccaagtcg gcagcagcca aaccatcgca tcacacgaaa accgcatcct cgccacaatg
     4261 tcctgccacg gctcaatccg cgccggccgc aggctcaccc tgcccgaaat gaacgcgctg
     4321 ctgcgcgata tggaaaatac gccgcgcagc aaccagtgca accacggcag gccgacgtgg
     4381 gtcaaactga ctttgaaaga attggacaca ctgttcttgc gcggacagta agccgaaaat
     4441 gctagaatac gccgcccgaa gccgccgttc agacggcatt ccgacgcacc gacagaaaca
     4501 tcacgaccga aaccaagaga aaaacatgac ctatcaagtt ctcgcccgaa aatggcggcc
     4561 caaaaccttt tccgacttag tcggtcagga acacgtcgtc aaagccctgc aaaatgcctt
     4621 ggacgaaggc aggctgcacc acgcctacct gctgaccggg acgcgcggcg tgggcaaaac
     4681 caccatcgcc cgcatccttg ccaaaagcct caactgcgaa aatgcgcaac acggcgaacc
     4741 ttgcggcgtg tgccaaagct gcacccaaat cgatgccgga cgctatgtcg acctgctcga
     4801 aatcgacgcc gcctccaaca caggcatcga caacatccgc gaagtcttgg aaaacgccca
     4861 atacgcgccg accgccggca aatacaaagt ctatatcatc gacgaagtgc atatgctttc
     4921 caaaagcgcg ttcaacgcca tgctcaaaac gctggaagag ccgcccgaac acgtcaaatt
     4981 catcctcgcc accaccgatc cgcacaaagt ccccgttacc gtattaagcc gctgcctgca
     5041 attcgtctta cgcaatatga ccgcgcaaca ggttgccgac caccttgccc acgtcctcga
     5101 cagcgaaaaa atcgcctacg acccccccgc cctgcaactt ttgggacgtg ccgccgccgg
     5161 atcgatgcgc gatgccttaa gcctgctcga ccaagccatc gccctaggtt cgggcaaagt
     5221 tgccgaaaac gatgtccgcc aaatgatcgg cgcggttgac aaacaatacc tgtacgaact
     5281 gctgacaggc atcgtcaacc aagacggcga agccctgctg gcaaaagcgc aggaaatggc
     5341 ggcgtgtgcc gtcggctttg acaacgcctt gggcgaactg gccatactgc tgcaacaact
     5401 cgccctgata caggcagtcc cctccgcctt ggcgcacgac gaccccgatt ccgatatttt
     5461 gcaccgcctc gcccaaacca taagcggcga acaaatccag ctttactacc aaatcgccgt
     5521 ccacggcaaa cgcgacctgg gcctcgcccc cgacgaatac gccggcttta tgatgaccct
     5581 gctgcgtatg ctggcgttcg cccccttggc ggcggcttcg tgtgatgcaa acgccgtgat
     5641 tgaaaatacc gaacttcaat ccccgtcggc acaaaccgcc gaaaaggaaa ccgccgcaaa
     5701 aaagccacaa ccgcgccctg aagcggatgc cgcccaaacg cccgttcaga cggcatccgc
     5761 agcagcaatg ccgtctgaag gcaaaactgc cggccccgtt tcccatcaag aaaacaacga
     5821 tgttccgcct tgggaagacg cgccggacaa aaccgaaacc gcagccggca cggcgcgaac
     5881 gtcggcaaaa agcattcaga cggcatccga agccgaaacg ccgcccgaaa atcaagtttc
     5941 caagaacaag gcagccgaca acgaaaccga ggcttccttg tccgaagtgc cgtctgaaaa
     6001 ccccattcag gcaacaccga atgatgaagc cgttgaaacg gaaacatttg cacacgaagc
     6061 ccctgcagaa cctttctacg gttttccgga taatgactgc cccccggaag acggcgtgga
     6121 aatcccgccg cccgattggg caaatgtcct ccctgccgat accgcaggcg gagggacgga
     6181 cgaagaagca gaagcgggcg gcatcggcgg aaacaacacg ccgtccgccc cgccgcccga
     6241 attttccacc gaaaactggg cagccatcgt ccggcacttc gcccgcaaac tcggcgcggc
     6301 gcaaatgccg gcgcaacact ccgcgtggac ggaataccgt tccgacaccg gactgatggt
     6361 tttggcaatg accgccgaag cccgcgccac cgccgacaaa aagcggctcg acaaaatccg
     6421 cgacaccctc gcccaagcct acgggctgca actcaccctg caaaccgaag actggcgcga
     6481 cgaagccggc cgggaaaccc ccgcgatgca ggacaagcgt gtccaagccg aagacaggca
     6541 aaaagcacaa gcattgctcg aagccgaccc cgccgcacaa aaaatcctcc aagcattcgg
     6601 cgcgcaatgg cagcccgaat cactggaatt ggcggcaaac cggccataaa cagatataat
     6661 gccgcccgaa cccttcggac ggcattgccg cttccctcat tcattcaaac acaaacagga
     6721 gtattcagta tgttcggaaa agccggatta ggcggcctga tgaaacaggc gcagcaaatg
     6781 caggaaaaca tgaaaaaggc acaagccaaa cttgccgaaa ccgaaatcga aggcgaagca
     6841 ggcaacggct tggtcaaaat cacaatgacc tgcgcgcacg aagtacgcaa aatcgacatc
     6901 agccccgatt tgattcaaga agccgccgat gacaaagaaa tgctcgaaga cctcatcctc
     6961 gccgccctca aatccgcccg gggcaaagcc gaagaaaccg caaacaaaac aatgggcgca
     7021 ttcacccaag atctgccccc cggcgtgggc gacttcttcc gctgaccccg accgtcattc
     7081 ccgcgaacgc gggaatccgg taaccgaaaa accacaggaa tctatcggaa aaaaacagaa
     7141 acccccgccg ccgtcattcc cgcgcaggcg ggaatctaga acgtagaatc tgaaaaaccg
     7201 ttttactcga taaatttccg tgccgacagg tctggattcc cgccttcgcg ggaatgacgg
     7261 catatttttt gcatttgata taaagggtcg tttgaatttt gttcagcaag tgcaaagtat
     7321 tgcacataaa agggcgcagg cttcatccgt aaagtcggcg caggatagag gcaaagcggg
     7381 cgtaggtcgg gctgtagcaa ctgtattttt caccccgtcg ggtaaaaata tagtggatta
     7441 acaaaaaccg gtacggcgtt gcctcgcctt agatcaaaga gaacgattct ctaagatgct
     7501 gaagcaccaa gtgaatcggt tccgtactat agtggattaa atttaaacca gtacggcgtt
     7561 gcctcgcctt gccgtactat ttgtactgtc tgcggctctc cgccttgtcc tgatttttgt
     7621 taatccgcta taccaaaact caaatcaagc cgtccggagg cggctcaaaa aaaacggtac
     7681 ttcgcagcag aagtaccgtt tatcgggatt tcaggtttta ttcttcgggt cgttcgccgt
     7741 cggtttcgtc ctgcgtccct tcggtgatgt gcatttctac gccgttgagg gcgcggattt
     7801 ttgcgtcgat ttcgtcggaa atttcggggt tttctttcag ccagacgcgg acgttgtctt
     7861 tgccctgacc gattttcgcg ccgttgtagc tgtaccacgc gccggatttg ttgatgatgt
     7921 cgtttttcac gccgatgtcg atcaattcgc cttcccaact gatgccttct ccgtagagga
     7981 tgtcaaactc tgcctgacgg aacggggggg cgactttgtt tttgatgact ttgacgcggg
     8041 tttcgttgcc caatacttct tcgccttttt tgatggatcc ggtgcggcgg atgtcgaggc
     8101 ggacggaaga atagaatttc agcgcgttgc cgccggtcgt ggtctcgggg ctgccgaaca
     8161 ttacgccgat cttcattcgg atttggttga tgaacacaac cagcgtattg gtttttttga
     8221 tgtgtccggt cagtttgcgc aaagcctgac tcatcaggcg cgcctgcagt ccgacgtggc
     8281 tgtcccccat atcgccttcg atttcggctt tggggacgag tgcggctacg gaatcgacga
     8341 ctaccatatc tatgccgccc gaacggacga gcgtgtcgca gatttccaaa gcctgttcgc
     8401 cggtatcggg ctgggacagg taaagctctt cgacttttac gccgagtttg cgggcgtaaa
     8461 cgggatcgaa ggcgtgttcg gcatcgacaa aggcgcacac gccgccgttt ttctggcatt
     8521 gggcgacggc ttcgaggcag agtgtggttt tgccggagga ttcggggccg aagatttcga
     8581 cgatgcgccc gcgcggcagg ccgccgactc cgagggcgag gtctaatccg agcgatccgg
     8641 tggaaatgac ttcgaggttt tcttcctgct ggctgccgtc cattttcatg atggcgcctt
     8701 tgccgaaact tttttcgatt tgcgccagtg cggcggcaag ggctttgctt ttgtcgtctg
     8761 acatgggggt tactccggaa caaatgcggt atgtgggctg cggcgcaaca cgggctgcgg
     8821 cgcggaatat gaatcggtcg gtcattatcg catgtttgcc tttcgggggc taccatccgc
     8881 ttcagaccga atgccgtctg aaacattgtc cctcctttgc tataatggcg cccttacttt
     8941 tcccgccagc cgcttgaacc gccatgcaca ccagcctgac cgtcaaaaat accgttatcg
     9001 gaagcgggcg caccaaaatc gccgtgccgc ttgtcgcccg agatgccgcc gatttatcct
     9061 ccgtgctgtc ccaaatcaaa aacctgccct tcgatatcgt cgagttccgc gccgactttt
     9121 tggaatgcgc gggcagcatc ggcgaagtat tgcgccacac gcaggccgtc cgcgacgcgc
     9181 tgcccgacaa accgctgctg tttacgttca gacggcattg cgaaggcggc tcgttcccgt
     9241 gttcggacga ttattatttt gaactgctcg acgcgctgat cgaaagccgc ctgcccgaca
     9301 ttatcgatat cgagctgttt tccggcgaaa ccgccgtccg ccgcgccgtg gcaaatgctc
     9361 aaaaaaacgg catcgccgcc ctgctctgca atcatgagtt tcaccgcacg ccgccgcaag
     9421 aagaaatcgt atgccgtctg aaacagatgg aggactgcgg cgcggacatc tgcaaaattg
     9481 cggtgatgcc gcaaagctcg gaagatgtgc tgactttgct ttccgccacg ctcgaagcga
     9541 aacggcttgt cgccaaaccg gttattacga tgtcgatggg gcagacgggt gcggtcagcc
     9601 ggcttgccgg acaggtgttc ggctcaagca tcacgttcgg ttcgggaacg caaaactccg
     9661 cgccggggca aatcggcgta tccgccctcc gtgcggcact cgactgcctc gaaagcggcg
     9721 cagactgatt tcagacggca tcaaaacatg atgaaactca atgcccaaca gctcgaagcc
     9781 gtccgctacc tcggcggccc cctgttcgtc cttgccggcg caggcagcgg caaaaccggc
     9841 gtgattacgc aaaaaatcaa gcatttgatt gtcaatgtcg gctatctgcc gcataccgtc
     9901 gccgcgatta cctttaccaa caaagccgct gcggaaatgc aggagcgcgt cgccaaaatg
     9961 ctgcccaaac cgcaaacgcg cgggctgacg atttgcacgt tccattcttt gggcatgaag
    10021 attctgcgcg aagaggcgaa ccatatcggt tacaaaaaaa acttctccat tctcgactct
    10081 accgacagcg ccaaaatcat cggcgaactc ttaggcggca cgggaaaaga agccgtattc
    10141 aaggcgcagc accagatttc cctttggaaa aacgatttaa aaacgcctga agatgtcgtt
    10201 cagacggcct ctaatgtgtg ggaacaacaa accgcgcgcg tgtatgcaag ttatcaggaa
    10261 accctgcaaa gctatcaggc agtagatttt gacgacttaa tccgcctgcc tgccgtgctg
    10321 ttgcagcaaa acagtgaagt gcgcaacaaa tggcagcggc ggctgcgtta tctgttggtt
    10381 gacgaatgcc aagatacgaa tacctgccaa tttgcgctga tgaaactctt gacaggcgcg
    10441 gaagggatgt ttaccgccgt cggcgacgac gaccagtcca tctacgcatg gcgcggcgcg
    10501 aacatggaaa acctgcgcaa aatgcaggaa aactatccgc agatgaaggt catcaaactg
    10561 gagcaaaact accgctccac cgcgcggatt ctcaaaattg ccaacaaagt catcgaaaac
    10621 aaccccaagc tgtttaccaa aaaactttgg tcgcaattgg gcgaaggcga gccggttaaa
    10681 gtcgttgcct gccaaaacga gcagcacgaa gccgattggg tcgtcagcca aatcgtcaaa
    10741 caaaaactca tcggcggcga caaaacccga tacgccgatt ttgccgtgtt ataccgcggc
    10801 aagcatcagg cacggatttt cgaggaagca ttgcgcggcg cgcgcatccc ctaccgtctc
    10861 tccggcggac aaagcttttt cgacaaagcc gaaatcaaag acgtgttgtc ttatgtgcgg
    10921 ctgcttgcca accccaacga cgatcccgcc tttctgcgcg ccgttaccac gccaaaacgc
    10981 ggcatcggcg atgtaacgct gggcaaactc aacgcctacg cgcacgaaca cgaatgcagc
    11041 ctgtatgaag ccgcacaaaa cgaagaagcc cttgccacac tgaacaatac caaccgccaa
    11101 cacctgcaag cctttatgga tatgttcggc aactaccgcg ccaaagccga aatcgacgaa
    11161 gcgggcgagt tcatcaacag cctgctcgaa gaaatcgact atgaaaacca tttgatgcaa
    11221 aacgaagaag gcaaagccgg cgaaatcaaa tggcgcaacg tcggcgagtt ggtatcgtgg
    11281 ttcgcacgaa aaggcgaacg ggacggcaaa aacgtcatcg aactcgccca aaccgtcgcc
    11341 ttgatgacgc ttttggaagg aaaagacgaa gaagaaaccg atgccgtctc gctatccacg
    11401 ctgcacgccg ccaaaggttt ggagtatccg tatgttttcc ttgtcggttg cgaagaaggt
    11461 gttttgccgc acaacgacag catcgaagaa ggcaacgtcg aagaagaacg ccgcctgatg
    11521 tacgtcggca tcacccgtgc caaacgccaa ctcacgctga cccactgtgt caaacgcaaa
    11581 aaacaaggca tatggcaatt ccccgaaccc agccgattca tagacgaaat gccgcaggaa
    11641 gatttgaaaa tcctggggcg caaaggcggc gaaccgattg tcagcaaaga agaaggcaga
    11701 cgtaaccttg ccgatataat cggaaggatc gacaacctaa aaaaaagcgg cccggcggat
    11761 taaaccggag ccgcaatgcc gtctgaaaga cgttcagacg gcatattttt tgaacggcgc
    11821 gcgtcaagcg gtttacgccc acaaatcctg ctgctgattt ttcggtacaa gcctgccgac
    11881 accgataccg atgaggcgga acgcgtcttc cgtctgcggc gggacgcgcg ccatcaacat
    11941 ttgcgcagcc tgcagcagag ctgcgcagtc gggcaatacg gaggaataag tcagcgtgcg
    12001 cgtgatgatg cggaaatcgt aggtcttcag cttgagcgtt acgctttggg cttcgacgtt
    12061 tttgcgcgtg atttgccgcc acaggtcttc ggcaagatgg ggcaggtgtc cggcagcctg
    12121 cccgagcggc aggtcttcgg gcagggtgat ttctgtggaa atttggaggc gttcgcgttc
    12181 ggctttgacg gggtgttcgt ccgtaccgcg cgccaaatca tagaggcggt agccgtagcg
    12241 tccgaaatga tttaagagct cgccgcgctc gaaacggcgc aaatcgcccg ccgtccgcat
    12301 acccagcgac tgcatttttt tcagcgttac cttgcccgct ccggggattt tgcccaaagg
    12361 cagggtttcc aaaaatgcca taactttgtg cggcggcaac acaaactgcc cgttcggctt
    12421 gcgccagtcg gaagcaattt tcgccagaaa tttgttcggc gcgatgcctg cggatgcggt
    12481 caaacccgtt tccgcaaaaa tggcggcacg gatttctttg gcaacttcgc cggcgtaagg
    12541 gatatttttg aaattacggg taacgtcaag ataggcttcg tccggcgaca agggttcgat
    12601 taaatcggta taacgcctga atacggcgtg aatctgcgcg gaaacctgac ggtacaaatc
    12661 gaaatgcgga ggcacataca ccgcttgcgg acacagcctt ttcaccgttg ccaccgacat
    12721 cgcggaatgc agcccgaact gccgtgcctc atacgatgcg gcgcaaatca ccgaacgcgc
    12781 gccctcccac gcgacgacca ccggccgccc tttcaaatgc ggctgctcgc gtagttctac
    12841 cgatgcgtag aacgcgtcca tgtcgatgtg gatgattttg cgtaaagaca tcggctctgc
    12901 cgaggataaa agggatattt tactgcgggc atcggacaaa atccaaatat actaacaggt
    12961 taaatacaaa agagttggct ggattaattt tgaaactcga taaaaataat ggtttttatc
    13021 aagcagaaag aaacagtaat ggaaattatg atatcgtaag aaaaaattat ctgaaaaata
    13081 ttaccaaaat acaatcaaat atattgattt acttagataa taccagtacc ggatttaaaa
    13141 ttcaacagag gagaaaacaa atcagagcac aaatttcagc cagacaatgg agaagataaa
    13201 agatgaataa aaatataatt tatatatttt ctttattaat cactattgtt attttcttta
    13261 tatttgaaaa gaatgtaata agcaaaataa gttttgatta taataaaaaa gaattcttaa
    13321 ttagtgatat aacgaacttt aactgggatc atgtaaaact gtatataatc aattcagatt
    13381 tccaaaaaat tgttttttat gataaaggta agatagtttt tgaggaatta atagaactag
    13441 ataggaaagg gaaagtttta cctcaatatc tatttgattc atatttgaaa aatgtttaat
    13501 attacgaatg tgattacaag aatggcaaga tgcaactttt aaaaaagaaa aattacattc
    13561 ttttgataga tatttttatt attataaacc tataaactgt aggccgaaac ttttgtaaaa
    13621 ctgtccatac tcattaccgc ctagtcggta atgcattttt tagtagtatt tgaaaaagta
    13681 tgatgataaa aacggttaag cctaactttt tcataaaatc cagcagagta aggcagaaaa
    13741 aaacacccaa attggctata atcccgacaa acacactcaa ggacaacaac atggcagcct
    13801 cgcccgaagc aaaattcacc gaagaaaaga ttttgtgggt caaacaccac acgccgaaac
    13861 tcatcacttt cgccatcagc cgtcccgaat cctaccgttt taaagccgga cagttctccc
    13921 gactcggttt ctacgaaggt aaaggcttta tttggcgtgc ctattccgtc gtttccgcag
    13981 aatatgccga cacgctcgaa tattttgccg tactcatcca agacggcccc atgtcggccc
    14041 ttttcgccaa aatgcaacag ggcgacacca tcctgctcga taaaaatgcc actggctttc
    14101 tcctgcccga acgcttcccc gacggcaagg atttggtgat gctctgtacc ggctcgggca
    14161 tcgccccctt cctttccatt ctcgaacaac ccgaaatccg gcagcgtttc gataccgtca
    14221 acctgataca ttccgtatct tttcccgaag aattgatttt caacgaccgg ctggccgcat
    14281 tgtccgaaca tcccctggta ggcgaatacg gacactcgtt ccgtttcgtc cctgttacca
    14341 cccgtgccgc caacccctcg ggcttaagcg gaaaacgcat tccggaactc ttaaaaaaca
    14401 acagcatcga acaggcgctg cataccaagc ttaccccgga atccacacgg tttatgattt
    14461 gcggtaaccc ggaaatggtc aaagacactt tccaaacgct gctcgatatg ggttacgcca
    14521 tgcaccgcaa ccgcattccc ggtcaaatca tgatggaaaa cggcttctaa aaacgaacgc
    14581 tcccaacccc tacccacgcc cacacacaaa acaaaataaa aaacagcgat atacaaaaac
    14641 caatttgcaa aaacagacct cacgcctata ctgccactca cagcaagcaa cacaaaaaag
    14701 cgggtacaaa ttagggtaca aaacacaagc ccgaacacag aagcacccca accaaaccca
    14761 aaggcaaagg caaacaaaat ggcggcacac aacaaactga cccaaaaaca aatagaagca
    14821 gccaaggcag acggcaaaca aagcaagctg gccgacggcg gcggcttata tctgctgctg
    14881 caccccaacg gcagcaaata ctggcggatg cgctaccggc acggcggatg cgaaaaaacc
    14941 ctagccttgg gcgtatatcc cgccgtcagc ctcaagcagg cccgcgaact tgcccgcgcc
    15001 gcccgggcgc agaccgccgc cggcatcgac cccgtcgccg aacaccaccg cacccgtccc
    15061 ggcagcgggc gcagccttcc cgaaatcgcc cgcgcgtggt acggcagccg tcaagggcaa
    15121 cgcgccgaca acacactgga aggcgaccgc cgttcactcg cctacctgac cgactactac
    15181 aaagacaaca ccgacatcaa cgacatcacc accgccggcg tatcaaaatt tatcgaacac
    15241 ctcaaccgca tcaacatccc cgcctgcgcc cggcgcaccg tccaaatcct cgcccaaata
    15301 tgggactatg ccgtccagcg cggcatcatt accgacgaac ggcgcaaccc cgcaaccacc
    15361 gcccgcccac tgctaaacac aagcaaaccc aaaccccagc cccacatccg ccccgacgag
    15421 ttgcccgact tttaccgcac cctccaaacc gcccacgacc tgcacccgta cgcccgcccc
    15481 ctgctgctgc ttgccgccct gaccgtcccc cgtccgtccg cactgctttc cgcccgctgg
    15541 caagacatcg acctcaccgc ccggttatgg cacatccccg ccgccgacat gaagaccaaa
    15601 caccccttta ccgtcccact gtcggactgg gcagtagaaa tactccgaga gctgcacaca
    15661 cagaccggcg acaacatcca ccttttccca ggcatccgcc cacggcgcaa acccgccccc
    15721 catcccgacc acatcagcat caaattcgcc cacaacgcca tccgacgact gggctatgac
    15781 ggcagcaccc cggcaaatcc aaacacacca tgcacggatt ccgcgacctc ttcaccaaca
    15841 tcagcctgac cgccggaaaa gacaccctca caacagacct cgcccttggc cacatcagcc
    15901 gcgccgccct ccaacgcgcc ggcttctcaa gcctgcacca ctacctgacc gccgacagct
    15961 accgcctcca agagcggcgc gaactcgccg aatggtacgg ccgccgccac cgccaagcat
    16021 acgaagccgc cgcccaaaac acttgcaata aaacctaaaa ggtattaaca tacatacatg
    16081 gcagacatcc tgccatccga caagcggcgg caaccgccaa aaacgaaagg aaatccaata
    16141 aacataatcg aacaagaaac aaaacgcacc gagttaagaa aaatagatgc cgaaatcgcc
    16201 aaaatcattg ccgacgcgca caaaatcaac gccgaatccg taaaaatagc ccaagagtcc
    16261 cgctggtatc ccatgatggc ggccaccggt ctggttaccg ccatcgccgc cgtgttggca
    16321 ctgatattca aatttgcctg accacaagcc ccgcgagggg ctttatccct caacaaaatg
    16381 aacagagccg cactattcaa ccgctacccc gaatggatta tcgggcaaga cggcgcaagc
    16441 cgtttcatta cccattgccg ctatccccgc ctgattgcca aaatccacag gcagacagac
    16501 ggcgaatgcc cgggcgggca ttaccgccat tccgaaaacg gcatcaccct ttacgatttt
    16561 atttttttcg gcggcaaacc ggcagacgaa gcccgatttg ccgccgtcct taccgaaacc
    16621 tgccgccggg cagtcaaaaa aa
//
LOCUS       DAXDXW010000054        16572 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXDXW010000054 DAXDXW010000000
VERSION     DAXDXW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16572
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.055"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..816)
                     /gene="cysW"
                     /locus_tag="ACFB64_001463"
     CDS             complement(<1..816)
                     /gene="cysW"
                     /locus_tag="ACFB64_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC1209807.1"
                     /translation="MKPYSANPNLTEPRRLRMLLIAAVLGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLSDPEAWSAVKLTLITALIAVPVNAVLGVAMAWLLTRFDFLGKQLLTT
                     LLDLPFSVSPAVAGLMFVLLFGAHTALGSRLEAQGIRIIFAIPGIVLTALFVTFPFAA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWTLLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTIPLLVEIFYNEYNFTGAFSLSGVLALLALATLAVQNIITK"
     gene            complement(1005..1834)
                     /gene="cysT"
                     /locus_tag="ACFB64_001464"
                     /pseudo
     CDS             complement(1005..1834)
                     /gene="cysT"
                     /locus_tag="ACFB64_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
     gene            2020..2352
                     /locus_tag="ACFB64_001465"
     CDS             2020..2352
                     /locus_tag="ACFB64_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209808.1"
                     /translation="MQILSFQADIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCAMVERGEFDEVEEFDGDDDAWLEAAKDCGHH
                     GDACACGH"
     gene            2682..3191
                     /locus_tag="ACFB64_001466"
     CDS             2682..3191
                     /locus_tag="ACFB64_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC1209809.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTV
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
     gene            complement(3423..4004)
                     /locus_tag="ACFB64_001467"
     CDS             complement(3423..4004)
                     /locus_tag="ACFB64_001467"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC1209810.1"
                     /translation="MEHKLPQLPYGPDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPVGGLDLISTFNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLEGFWEIVNWDEVAKRFAA"
     gene            4167..5570
                     /gene="dnaB"
                     /locus_tag="ACFB64_001468"
     CDS             4167..5570
                     /gene="dnaB"
                     /locus_tag="ACFB64_001468"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC1209811.1"
                     /translation="MNDYAAMPPEGREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINEGRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGRLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFADLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEYVAIEKHLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVEQRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKVILTWMGQ
                     FTKFGNAAYIPESAAED"
     gene            5827..6489
                     /locus_tag="ACFB64_001469"
     CDS             5827..6489
                     /locus_tag="ACFB64_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFC1209812.1"
                     /translation="MCTRKQQGFTLTELLIVMAIAAIMATIALPNMSGWIASRRIASH
                     AEQVANLLRFSRGEAVRLNLPVYICPVQVKKDGASNNRCDFSKKGWGMLAFGDKNDNK
                     AYDGDATDVFLRSVVLNDTDDSRINYAFNHIAFGQTRSTADRVVWTFNQNGTFGYLPD
                     QNLKNNSKFVYSDGYIQIVLTDARAVSDADRKFRSAVVLIDSSGRVEVCRKNDTRAVC
                     KH"
     gene            6521..7129
                     /gene="pilV"
                     /locus_tag="ACFB64_001470"
     CDS             6521..7129
                     /gene="pilV"
                     /locus_tag="ACFB64_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC1209813.1"
                     /translation="MKNNDCLRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYTEPYTSTPSGGDSKPNTL
                     ISKRDLAKVQLDRFDYELRNALPDAVAIHYAVCKDSSGNAPTLSGDTFSPNCDDKANG
                     DTLIKVLWVNDSAGDSDISRTNLEVSGDNIVYTYQARVGGRE"
     gene            7126..8058
                     /locus_tag="ACFB64_001471"
     CDS             7126..8058
                     /locus_tag="ACFB64_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC1209814.1"
                     /translation="MKRKMLNVPKGGYDGMKGFTIVEFLVAGLLSVIVLIAVVSSYFT
                     SRKLNDAANERLAEQQDLRNAATLIVRDARMAGSFGCFNMSEHIKDVVVSDVAQKNYL
                     FLLKKSNANKLIPIAESSNINYRGFLQVGSALIFQYGIDDVNASAATTVVSSCAAISK
                     PGKQIPTLEDAKKELKIPDRDKEQNGNIARQRHVVNAYAVGRIDGEGGLFRFQLNEKG
                     EWGNPQLLAKKIKRMRVRYIYVSGCPEDEDAGKEEQFKYTDKFDSSVTPAGVEVLLDS
                     GSDAKIAASSDNIIYAYRINATIRGGNVCANRTL"
     gene            8037..8648
                     /locus_tag="ACFB64_001472"
     CDS             8037..8648
                     /locus_tag="ACFB64_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC1209815.1"
                     /translation="MRKQNTLTGIPTSDGQRGSALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGEFQVLDLEYAADSKVTFSENCEKGLCTAVNVRT
                     NDNGNEEAFGNIVVQGKPTVEAVKRSCPAKSGKNSTGLCIDNQGVEYKKGTGNVSKMP
                     RYIIEYLGVKNGQNVYRVTAKAWGKNANTVVVLQSYVGNNDEQ"
     gene            8650..9123
                     /locus_tag="ACFB64_001473"
     CDS             8650..9123
                     /locus_tag="ACFB64_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC1209816.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNVLKQFILKNPQDNNQIIKSKLETFVLGYKMNPKIAKKYSVSVAFANTEKPRA
                     YRLVGVPKAGTGYTLSVWMNSVGDGYKCRNATSAQTYSETLSANTGCEAFSNRKK"
     gene            complement(9193..9501)
                     /locus_tag="ACFB64_001474"
     CDS             complement(9193..9501)
                     /locus_tag="ACFB64_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD family protein"
                     /protein_id="HFC1209817.1"
                     /translation="MKDLLSFPSFLLFSCMLAVTCPTQLIGFFALRNRTLSRRAQTVM
                     ETAPGCVLISVIAPYFVSDKPHELIAIALTAFAACRFSMLFTVLIGVGSSGISGWLMA
                     "
     gene            complement(9498..10209)
                     /locus_tag="ACFB64_001475"
                     /pseudo
     CDS             complement(9498..10209)
                     /locus_tag="ACFB64_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            10375..10827
                     /gene="dut"
                     /locus_tag="ACFB64_001476"
     CDS             10375..10827
                     /gene="dut"
                     /locus_tag="ACFB64_001476"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC1209818.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     GTLLVPTGLAIYLANPAYTAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     GSEPFTVKPFERIAQMVIVPVVQARFKRVEEFVGSSRGEGGFGSTGSH"
     gene            10905..12092
                     /gene="dapC"
                     /locus_tag="ACFB64_001477"
     CDS             10905..12092
                     /gene="dapC"
                     /locus_tag="ACFB64_001477"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC1209819.1"
                     /translation="MNTLLNRLKPYPFARLREAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLRELEKYPLTAGLPELRQACANWLKRRYDDLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWERTKLVFVCSPGNPSGSVPDLDGWREVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQSGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWNDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVC
                     LYR"
     gene            12248..13027
                     /gene="yaaA"
                     /locus_tag="ACFB64_001478"
     CDS             12248..13027
                     /gene="yaaA"
                     /locus_tag="ACFB64_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC1209820.1"
                     /translation="MFFVLSPAKNLNEKDPCPVSEFTQPDLLAESEILMRQLRELAPQ
                     QIAELMHVSDKIALLNAERNAAWHTPFTPENAKQAVFMFNGDVYEGMDANTLNTNQIQ
                     YLQNHVRLLSGLYGLMRPLDLMQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            13110..13200
                     /locus_tag="ACFB64_001479"
     tRNA            13110..13200
                     /locus_tag="ACFB64_001479"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13144..13146,aa:Ser,seq:tga)
     gene            13277..13366
                     /locus_tag="ACFB64_001480"
     tRNA            13277..13366
                     /locus_tag="ACFB64_001480"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13311..13313,aa:Ser,seq:cga)
     gene            13557..14757
                     /locus_tag="ACFB64_001481"
                     /pseudo
     CDS             13557..14757
                     /locus_tag="ACFB64_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(15113..15382)
                     /locus_tag="ACFB64_001482"
     CDS             complement(15113..15382)
                     /locus_tag="ACFB64_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209821.1"
                     /translation="MESSDVQGRMETLAFATAPKKGGIRKSGFGRVILIKSGHIVIHN
                     ADGDSFATGYGVIAADIVSPDLKTDFDIFFPPESLLRYKKIGKKK"
     gene            complement(15577..16260)
                     /locus_tag="ACFB64_001483"
     CDS             complement(15577..16260)
                     /locus_tag="ACFB64_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFC1209822.1"
                     /translation="MDKEKVLDKIKKCLALGRSANEHEAAQALRQAQALMEKYKVNAE
                     DIALSKVSEQKADRKMAFKLAGWQWGVANMIADIFGCKSYQRGKTMMFYGIGNRAETS
                     AYAFDVVYRQISADRRKFLKTCRAGKPSHRTYLADRFCGGWIASAWETVKKFEMSDEE
                     KAIMDGYKKKEYPDMAEARTRDAKSSILQGSKMEYEALTRGMESGKQVKLHYAVNGTG
                     FVKQIGGQT"
     gene            16385..>16572
                     /locus_tag="ACFB64_001484"
     CDS             16385..>16572
                     /locus_tag="ACFB64_001484"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209823.1"
                     /translation="MFPPAEITGRGRVVLQQGFAGFGLYFHGVKFRRLNIPFKLPASC
                     RAVKYARMFAAVSADFGG"
BASE COUNT         4146 a         3864 c         4360 g         4202 t
ORIGIN      
        1 tttggtgatg atgttctgca ccgccagcgt cgccagtgct aaaagtgcca atacgccgga
       61 gagggagaat gcgccggtga agttgtattc gttgtagaag atttcgacca aaagcgggat
      121 agtgttggtt tcgccgcgta tgtgtcccga taccacgctg accgcgccga actcgcccat
      181 cgcacgggcg ttggtgagga tgatgccgta gagcagcgtc catttgatgt tgggcagggt
      241 aacgcgccaa aacatctgcc aaccgcttgc gccgagtatc aatgccgcct gttcttcgct
      301 gtcgccctgt gcctgcatta aagggatgat ttcgcgtgcg gcaaagggaa aggtaacgaa
      361 cagcgccgtc aaaacaatgc cgggaatggc gaagataatc cgtatgcctt gcgcttccag
      421 ccggctgccc aacgccgtgt gcgcgccgaa caataagacg aacatcaaac cggcaaccgc
      481 gggcgatacg gaaaacggca aatcgagcag ggtggtcagc aactgcttgc cgagaaagtc
      541 aaaacgggtc aacagccacg ccatcgccac gcccaagacg gcattgacgg gaacggcaat
      601 cagcgcggta atcagcgtca atttgacggc agaccacgct tcgggatcgc ttaaggattt
      661 caggtacaaa tcccaaccgc cttttaaggc ttcgtaaaac acggcgacga gcggcacgac
      721 cagcatcagc agcagaaagc ccagcacggc ggcaatcagc aacatgcgca gccggcgcgg
      781 ttcggtcagg ttgggattgg cggaataggg tttcatggcg gtggtgttct ctggtttgtt
      841 gtttaaggtg ttcggatgcc gtctgaaagg gattgagggg tttcagcttt cgtcggtcgg
      901 attcttgaat ccgacaaggt tgctgaatcg caaatgtttg tcggatacaa gtatccgacc
      961 tacgaattct gtgttttggg gagcggattc gttttcagac gacctcaacc cttcgcaccc
     1021 gaacgcctgc ccaacgccca ctgcagcacg ttcagcgcaa acagaatcac aaacgaaacc
     1081 agcagcataa acaacgccac cgccgacgcg ccctgcgcgt cgaattgttc cagcttgccc
     1141 gtaataatca gcggcaggat ttcggaaatc atcggaatgc tgcccgcgat aaaaatcacc
     1201 gaaccgtatt cccccgttgc ccgcgcgaac atcatgcccg cgccggtcaa gagtgccggc
     1261 gtgatttccg gcagcagcac gcggcggaac gttgtgaaac ggtttgcgcc caaagtcgcc
     1321 gccgcttcct catattcgcc cgacaattcc ttcaataccg gctgcacggc gcggacgata
     1381 aagggcaggc tgacgacaac cagcgcaatc caaatgccga cgggcgtaaa cgcgattttg
     1441 atgcccaaag gttcgaaaaa acggcctatc caaccgttgg gcgcatacag ggccgccaac
     1501 gcgatacccg taaccgccgt cggcagcgca aacggcaaat caaccagcgc gttcgccagg
     1561 cccttgcccg gaaattcata gcgcaccaac acccaagcca ccagtgtgcc gaacacgaca
     1621 cattggtcag catcgcataa aacgacatcc gcaaactcag ccgcaccgcc gccagcacgt
     1681 tcggctcggc aatcgtgttc cgaaagccgc cccagccgat ttccgccgcc atcatcgcaa
     1741 acggcaaaac caccagaagc gacaggcaca atacggtcag accgaggctg agtttgaagc
     1801 cgggcagtac gccgggtgtt ttgagcggta acataaaaca atgctgaaaa taaggaaaag
     1861 gaaggactac tttaacaatg ccgcctgaaa aacggaaaaa atggaaagtt tggtgcaaag
     1921 acgaatttgt tataaagcgg ttggcagttt ttcaagcggg cgcgatgttc taaaataacg
     1981 ctttgttttg acggaaaacc atcatataaa ggaacactta tgcagatttt atcttttcag
     2041 gcggacattg cggaacgtat gctggaaggt acggaaggcg agtcggtcaa cgaaaacgca
     2101 caatttgtcc gtacggacaa cggctattgg attgcgtggc atgaaggcgt ggcggcactg
     2161 cttgcgcccg atacgccgcc gggcattccc tgtttttggg tggaaggggc ggaaagcctt
     2221 gaagagttgt gcgcgatggt ggaacgcggc gagtttgacg aagtggaaga gtttgacggc
     2281 gatgacgacg cgtggctcga agcggctaaa gattgcggac accacggcga cgcttgcgcc
     2341 tgcggacatt aaaggcatgg caggcttgcc gcaaggggtg caaggctttg ccgtttttta
     2401 aataatcggc ggattgcatc tgtttgcaat gggcggaacg cttccctttg tattttattg
     2461 aataaaaatt atcttggtat tataataagg cagcatcaat tattttggga ctgcaacaaa
     2521 cgcaaagcat tgatttgcgt ttgttgcaaa cttattatta tagcaggttg cggcgcggac
     2581 ttgatgggaa ttgtttatag agcatgatag tctgaaacat attaattaaa aataccatta
     2641 atccgattta tatttatttc aatttcaatg gaaaaacatc aatgacaatg attttaagca
     2701 ttttaagcct gttttttatc attagactgt tatttttagc cgtctccatt aaacatgaaa
     2761 aagccttgat tgccaaaggg gcgaaacaat acggaaaaac caattccacg gtgcttgcgg
     2821 cagttcatac gctttattat ttggcgtgtt ttgtttgggt atggctttct gacactgctt
     2881 ttaatggcat atccttgatt ggtacactga cggtgatggc ttcatttgtg atattatcat
     2941 tgattattaa gcagttgggg gagatttgga cggttaaaat ctatattttg ccaaatcatc
     3001 aaattaatcg ttcgtggttg tttaaaacat tccgccaccc gaattatttt ttaaacatca
     3061 tacccgaact gattggcatc gccttattat gtcaagcgtg gtatgtttta ttgattggcc
     3121 tgcccattta tttgctggtc ttatttaagc gtatccgaca agaagaacag gcgatggcaa
     3181 cactttttta acccgtttca tcaattaaaa atatatcgac aaagagaacc gtctatcaat
     3241 taaatccatt cttatcattg gggcaggctt attgtcattg ggcaagccgt gacaaaagcc
     3301 cgaacctgca aaacttatct ataaggctgc atcagtgcgt attgaagccc cgcctttttt
     3361 gacaaaaaaa tgccgtctga aggcttcaga cggcatttgt tcggtttggc aaagccggcg
     3421 gcttatgcgg caaaacgttt ggcgacttcg tcccagttga cgatttccca aaaaccttcc
     3481 aggtagttgg ggcggctgtt gcggtagtcg atgtagtagg cgtgttccca cacgtcgcaa
     3541 gtcaagagcg gcgtgttttc agtggtcagc ggcgtagcgg cgttgaaagt ggaaatcaaa
     3601 tccaatccgc cgacaggggt tttcaccagc cacgcccaac cggagccgaa cgtaccggcc
     3661 gcgcaggcat tgaacgcttc ttggaatttt tcgaagctgc cccatttcgc gtcgatggcg
     3721 gcggccagtt cgccggcagg cttgccttgg cctttgggcg tgaaacccag ccagtagaag
     3781 gtgtgattcc aagtttgagc cgcgttgttg aacacgccgc ccgaagattt tttcacaatc
     3841 tcttccaaag gcaggttttc aaattcggtg cctttgattt gattgttcag gttggtgatg
     3901 taggtttgat ggtgtttgcc gtagtggaat tccaaagtct ctttgctcag atgcggggac
     3961 aatgcgtccg gcccataagg cagttgcggc agcttatgtt ccactttgtg ctcctaatat
     4021 tgttttaaat gttgtatttt ggcagtgttg ctgcaaacga atcggcagct cacgcattct
     4081 acctgttttg agtgatggaa accaattaaa cctgctttgc gttataatag aagattgcaa
     4141 tttcggcatg acagattgga tgtaccatga acgattacgc agctatgccg cctgaaggcc
     4201 gtgaggtcgg cgcattgtcg ctgcctccgc attcgatgga ggcggaacaa tccgttttgg
     4261 gcggtttgat gctggaaaat ccggcttggg acaggattgc cgatgtggtt tcgggagagg
     4321 atttctaccg ccacgaacac cgcctgattt tccgatccat tgccaaatta atcaatgagg
     4381 gccgtcccgc agatgtgatt acggttcagg aagatttgca gcggaacgaa gaattggaag
     4441 cggcaggcgg atttgaatat ctgattacac tggcgcaaaa caccccgtct gccgccaaca
     4501 tccgccgcta cgccgaaatc gtacgcgagc gttccattat gcgccaactc gccgaagtgg
     4561 ggacggaaat cgcccgcagc gcatacaatc cgcaaggcag ggacgcgggg cggcttttgg
     4621 atgaggcgga aaacaaagtg tttcaaatcg ccgaaagcac cgccaaatcc aagcagggct
     4681 ttttggagat gcccgatttg ctgaaagaag tcgtacagcg catcgatatg ctttattcgc
     4741 gcgacaatcc cgatgaagtt accggcgtgc cgacagggtt tgccgacctc gataaaaaaa
     4801 catccggcct ccagcccggc gacctgatta tcgtcgccgg ccgcccgtct atgggtaaaa
     4861 ccgcgttctc catcaatatt gccgagtatg tcgccattga aaaacatttg cccgtcgccg
     4921 ttttctcgat ggaaatgggc ggtgcgcagt tggtcatgcg tatgctcggc tcggtcggac
     4981 ggctggatca aagcgttttg aaaaccggca ggctcgaaga cgaacactgg ggccgtctga
     5041 acgaggcagt cgtcaaactc tcagacgcgc ccgtgtacat cgacgagacc ccgggcctga
     5101 ctgcgctcga actgcgcgcc cgcgcccgtc gtctcgcccg tcaatttaac aataagctgg
     5161 gattaatcgt cattgactac ctgcaactga tggcgggatc cggccgttcg gacaaccgcg
     5221 cttccgagct tggggaaatt tcccgttccc tcaaagcttt ggcaaaagag ttgcaagtcc
     5281 ccatcatcgc cctgtcgcaa ttgagccgca ctgtcgaaca gcgcaccgac aaacgcccga
     5341 tgatgtccga cctccgcgaa tccggtgcta tcgagcagga tgccgacctg attatgttta
     5401 tgtaccgcga cgaatactac aaccaggact cacccatgaa aggccttgcc gaatgtatca
     5461 tcggcaaaca ccgcaacggc ccggtcggaa aagtcatcct gacgtggatg gggcagttta
     5521 ccaaattcgg aaatgccgcc tatatcccgg aatcggcagc ggaagactga atggcatagt
     5581 atagtggatt aacaaaaacc agtacggcgt tgcctcgcct tgccgtacta tctgtaccgt
     5641 ctgcggcttc gccgccttgt cctgattttt gttaatccac tataaagtaa aaaaaattat
     5701 taggcaaaaa cgggtaaaat cgtttagaat tgcctatgcg gttttcctaa aaataaaagc
     5761 ggcgggatgc cttccatccg gcctgcagat aattgaaaat acattacagt tgacgtccta
     5821 ttgcttatgt gtacacgaaa acaacaaggt ttcacgctaa cagagctgct catcgtgatg
     5881 gccatcgcag ccattatggc gacgatagcc ctccccaata tgagtgggtg gattgcatca
     5941 cgccgcattg ccagtcacgc ggagcaggtt gccaaccttt tgcgtttctc caggggcgaa
     6001 gccgtccggc tcaatctccc tgtctatatc tgtcctgttc aagttaaaaa agacggtgcg
     6061 tccaacaata gatgtgactt cagcaagaag gggtggggaa tgttggcttt cggcgacaaa
     6121 aacgacaata aggcatatga cggcgatgcg acggatgttt tcctccgcag cgtggtgttg
     6181 aatgataccg acgacagccg gattaattac gccttcaatc atatcgcttt cggtcaaaca
     6241 cggtcgactg ccgatcgtgt tgtttggact ttcaaccaaa acgggacatt cggctatttg
     6301 cccgatcaga atctcaagaa taattccaaa tttgtttatt ctgacggtta tatccaaatc
     6361 gtgctgacag atgcgagagc agtttcagat gccgatagga aattccgttc ggcggtggtt
     6421 ttgattgaca gcagcggcag ggttgaagtt tgtcgtaaaa acgatacgcg cgccgtatgc
     6481 aaacattaat gactgtttta gtcggtttgg gaaataaaaa atgaagaata atgattgctt
     6541 gcgcctgaaa aatccccagt ccggtatggc gttgatagaa gtcttggtcg ctatgctcgt
     6601 tctgaccatc ggtattttgg cattgctgtc cgtacagttg cggacggtcg cttccgtcag
     6661 ggaggcggag acacaaacca tcgtcagcca aatcacgcaa aacctgatgg aaggaatgtt
     6721 gatgaatccg accattgatt cggacagcaa caagaaaaac tataatcttt acacggagcc
     6781 gtacacctcc actccctctg gcggcgattc caagcctaat actttgataa gtaagaggga
     6841 tttggcaaag gtccagttgg acaggttcga ttatgaattg agaaacgcct tgccggatgc
     6901 ggtagctatt cattacgccg tctgcaagga ttcgtcgggt aacgcgccga cattgtccgg
     6961 cgatactttt tctccaaatt gcgacgataa ggcaaacggg gatactttga ttaaagtatt
     7021 gtgggtaaat gattcggcag gggattcgga tatttcccgt acgaatcttg aggtgagcgg
     7081 cgacaatatc gtatatacct atcaggcaag ggtcggaggt cgtgaatgaa acgtaaaatg
     7141 ctaaacgtac caaagggcgg ttatgatggt atgaagggtt ttaccattgt tgaatttctg
     7201 gttgcgggcc tgctcagtgt gattgtcctg atagcggtcg tatcgagtta ctttacatcc
     7261 cggaaattaa atgatgcggc aaacgagcgt cttgccgagc aacaggattt gcggaatgcg
     7321 gcaacattga ttgtccgcga tgcaagaatg gcggggagct tcggttgctt caatatgtct
     7381 gagcatatca aagatgttgt tgtttccgat gtggcgcaaa aaaactatct ttttttgtta
     7441 aaaaagagca atgcaaataa acttatcccc atagcggaat cttcaaatat caattatcgg
     7501 ggttttctcc aggttggtag cgcattgatt tttcaatacg gaatcgatga tgttaatgca
     7561 agcgccgcga ctaccgtcgt cagcagctgt gccgcaatat cgaaaccggg caagcaaatc
     7621 cctactttag aagatgcaaa aaaagaattg aagattccgg atcgggataa ggagcaaaat
     7681 ggcaatatag cgcgtcaaag gcatgtggtc aatgcctatg cggtcggcag gattgacggt
     7741 gagggaggtt tgttccgctt ccaattgaat gaaaagggcg agtggggtaa tcctcagttg
     7801 ctcgcgaaaa agattaaacg tatgagagtg cggtatatct atgtttccgg ttgtcctgaa
     7861 gatgaagatg ccggcaaaga ggaacaattc aaatatacgg ataaattcga cagctctgtt
     7921 acgcctgccg gggtggaggt tttattggat agcggtagtg atgccaagat tgccgcttct
     7981 tcagataata ttatttatgc ttaccgtatc aatgcgacaa tacgcggggg aaatgtatgc
     8041 gcaaacagaa cactttgaca ggaatcccga cttctgacgg acagaggggg tccgcactgt
     8101 ttatcgtgct gatggtgatg atagtcgtgg cctttttggt tgtaactgcc gcccagtcct
     8161 acaataccga acagaggatc agtgccaacg aatcagacag gaaattggct ttgtctttag
     8221 ccgaggcggc tttgcgggag ggcgaatttc aggttttgga tttggaatat gctgcggaca
     8281 gtaaggttac gtttagcgaa aactgtgaaa aaggtctgtg taccgcagtg aatgtgcgga
     8341 caaatgataa tggtaatgaa gaggcttttg gcaatatcgt ggtgcaaggc aagcccaccg
     8401 ttgaggcggt gaagcgttct tgccctgcaa agtctggcaa aaattctacc ggcctgtgca
     8461 ttgacaatca gggagtggaa tataagaaag gtacgggaaa cgtcagcaaa atgccgcgct
     8521 atattatcga atatttaggc gtgaagaacg gacaaaatgt ttatcgggtt actgccaagg
     8581 cttggggtaa gaatgccaat accgtggtcg tccttcaatc ttatgtaggc aataatgatg
     8641 agcaataaaa tggaacaaaa agggtttaca ttgattgaga tgatgatagt tgtcgcgata
     8701 ctcggcatta tcagcgtcat tgccatacct tcttatcaaa gttatattga aaaaggctat
     8761 cagtcccagc tttatacgga gatggtcggt atcaacaatg ttctcaaaca gtttattttg
     8821 aaaaatcccc aggacaataa tcagatcatc aagagcaaac tggaaacatt tgtcttaggc
     8881 tataagatga atccgaaaat tgccaaaaaa tatagtgttt cggtggcatt tgccaatacg
     8941 gaaaaaccaa gggcatacag gttggtcggc gttccgaagg cagggacggg ttataccttg
     9001 tcggtatgga tgaacagcgt gggcgacgga tacaaatgcc gtaatgccac ttctgcccag
     9061 acctattcgg agaccttgtc cgcaaatacc ggctgtgaag ctttctctaa tcgtaaaaaa
     9121 tagggttgtt ttgccaatac cgtctgaaga tcaattttca gacggtattt tttatgggca
     9181 ttttgaaatg aattatgcca tcaaccatcc cgagatgcct gacgagccga cccctatcaa
     9241 tacggtaaac aacatagaaa aacggcaggc cgcaaaggcg gttaacgcga tggcaataag
     9301 ttcgtgcggc ttgtcggaga caaaataggg ggcaataacg gaaatgagga cgcagcccgg
     9361 cgcggtttcc ataacggttt gggcgcggcg gcttaaggtt cggttacgca atgcgaaaaa
     9421 gccgattaac tgggtagggc aggtaacggc aagcatacag gaaaacagca ggaatgatgg
     9481 gaacgacaat aaatctttca tttccgccca ttccaaaaat aggcggacaa caggccggac
     9541 agcgtgccgg cgggaacata ccacgcgccg tcaacggtca ggtaaacggc ggatgcgaca
     9601 aacaggttga caaaccaaag tcttgatgcg gaaaaacttt tccacattcc cctcagtaaa
     9661 accaaaaata cggcaggaaa cgccataccg aacccccaag cggccacatt accgaacacg
     9721 ggccctactg ccgcgccgag ggcggcaaac cctatccagg taatgtagag gataaagcat
     9781 gcgcccatat aaaaaggcat attgaatgca ggcaaaccgg ctgctttccg tttttggatt
     9841 tcggagaatg ccatggcccg gctttcatca cacataaaaa gcagcgcggg cacggctttt
     9901 ttcagcggta tttctttcat gtgcgtggca agcgccgccc cccatcagga tatgccgcga
     9961 attaatcata aaggttatgg tggcgatagg cagtatcggc agaggttccg cccacaggtt
    10021 gaccgtggca aattcggagc cgccggcgaa gttcatcccc gtcatcagca gcatttccag
    10081 ccggcccata cctttttgcc cgccctgcat tccgagtatc aatgcccaag gcaaaagccc
    10141 aatcagcatg ggcgaacttt ctttgatgcc gcgtatgaat tcatcacgtg gagaggctgt
    10201 gtgcgtcatg ttaatgttta tatatgaaga gacggggcgg tatcatacac aggcagaatg
    10261 tttttgacag tcgaggatgg tttcaaaaca aaagcagttt gttgtgtttc agcgtaaaca
    10321 cggcttgtgt ataatctcca atctttgaaa ccggccgtat gcaggagcag gacgatgaat
    10381 attgaagtag aaatgaaagt gttggacgag aggatggcgg attttatccc tgcctatgcg
    10441 acggagggtt ctgcaggttt ggatttgcgc gcctgtttgg atgaggaagt cgttttgcag
    10501 ccgggcggaa cgttgcttgt gccgacaggt ttggcaattt atttggcgaa tcccgcatat
    10561 accgccgttt tgctgccccg ttccggcttg gggcataaac acggcattgt tttgggcaat
    10621 ttggtcggct taatcgactc cgattatcag ggagagttga aggtgtcgtt atggaatagg
    10681 ggtagcgaac cgtttactgt caaaccgttc gagcgtatcg cacaaatggt tatcgtgccg
    10741 gttgtgcagg cgcgtttcaa acgtgtcgag gaatttgtcg gaagcagccg gggtgagggc
    10801 ggcttcggca gtacgggttc tcactaaaga tatagaatgc cgtctgaagg gcacgtcagc
    10861 ttcagacggc atatcttccg atataccgat aaccggagaa aatcatgaat accttactca
    10921 accggctcaa accatatccc tttgcccgac tgcgcgaggc gatgcagggc atttccgcgc
    10981 ctgaaggcat ggaagccgtc ccactgcata tcggcgaacc gaaacacccg acaccgaaag
    11041 tcattacgga tgcgctgacc gcctcattgc gcgagttgga aaaatatccg ctgactgcgg
    11101 gtctgcctga actgcgtcag gcgtgtgcga actggttaaa acgccgttac gatgatttga
    11161 cagtggatgc ggataatgaa attttgccgg ttttaggcag cagggaagcc ctgttttctt
    11221 ttgtccaaac cgtgttgaac cctgtttcag acggcctaaa acccgtaatc gtcagcccga
    11281 atccctttta tcagatttat gaaggtgcga cacttttggg cggcggtgaa atccattttg
    11341 ccaattgccc cgcgccgtct ttcaaccccg attggcgcag tatttccgaa gaggtttggg
    11401 aacgcaccaa actggtgttc gtctgctcgc ccggcaatcc cagcggcagc gtgccggatt
    11461 tggacggatg gagagaagtt tttgatttac aggataaata tggtttcatt attgcctcgg
    11521 atgaatgcta ttccgaaatc tatttcgacg gcaacaaacc tttgggctgc ctgcaggccg
    11581 ccgcacagtc ggggcgaagc aggcaaaaac tgcttatgtt taccagcttg tccaagcggt
    11641 ccaatgttcc gggcctgcgt tccggttttg tcgccggtga tgcggaactg cttaagaact
    11701 ttctgcttta cagaacctat cacggcagcg caatgagcat tcccgtgcag cgtgccagta
    11761 ttgccgcctg gaatgacgaa caacacgtta tcgacaaccg ccgcctgtat caggaaaaat
    11821 ttgagcgtgt tattcccatt ttgcaacagg tatttgacgt taaattaccg gatgcctcgt
    11881 tttacatctg gttgaaagtc cccgatggcg acgatttggc attcgcacgc aatttatggc
    11941 aaaaagccgc tatccaagta ttgcccggac gctttttggc acgggatacc gaacagggca
    12001 atcccgggga aggttatgtg cggattgctt tggttgcaga tgtcgcaact tgtgtcaaag
    12061 ctgcggaaac cattgtttgc ttatatcggt aaagaataaa aaatgccgtc cggacttttc
    12121 ctgacggctt tttttaatac tatcaagatg tatttattat aacggtattt acatcacaat
    12181 caatcggctt gcctgaggat gcttttaaat ctaaaattcc gacgtttgac actataccga
    12241 gcggattatg ttttttgtcc tttcccctgc gaaaaacctt aacgaaaaag acccttgccc
    12301 tgtcagcgag tttacccaac ccgatctttt ggcggaatct gaaatcctga tgcgccaact
    12361 gagggagctt gcccctcaac aaattgcaga gttgatgcac gtttccgaca aaatcgcact
    12421 tctaaacgcc gaacgaaatg cagcgtggca cacgccgttt acgccggaaa acgccaaaca
    12481 ggcagtcttt atgttcaacg gcgatgttta cgaaggtatg gatgcaaaca cattgaatac
    12541 caatcaaata caatatctgc aaaaccatgt ccgccttctg tccggcttgt acggtcttat
    12601 gcgtccactg gatttgatgc agccctaccg cctggaaatg ggaacggcat ttgccaattt
    12661 gcgcggcaag aatttgtatg agttttgggg cgacatcatt accaaccttt taaatgacac
    12721 gcttgcccaa gcaggcagca atacgcttgt caaccttgcc tcacaggaat atttcaagtc
    12781 cgtcaacacg aaaaaacttc gggcgcggct gattaccccg atatttaaag acgaaaaaaa
    12841 cggtaaatat aaaatcatca gtttctatgc caaacgcgcg cgcggattga tggtgcgcta
    12901 tgcggcagaa cacaatatta ccgatcctga aatgctgaaa aattttaatt acgaaggcta
    12961 cgcattcaat gacgcggctt ccaatgaaag cgaatgggtg tttatgcgtt cggaacaaat
    13021 aaagtgaaaa caataaatta agtatttccc aaaaaaagtg cttggcaaaa tgtataaact
    13081 tcattattat tcctactctt caagaagacg gaagcgtggc agagcggttt aatgcaacgg
    13141 tcttgaaaac cgtcgagggt tgatagccct ccgtgagttc gaatctcacc gcttccgcca
    13201 atttttgagc gtaaaaccaa ataaaaatgc aatagccgcg aatattgtta tttttatttg
    13261 gttttactgc attatcggaa acgtggcaga gaggctgaat gcagcggact cgaaatccgc
    13321 tgagggtgca aatcctccgt gggttcgaat cccaccgttt ccgccagaaa acataaccgc
    13381 cctgattcag ggcggttctt ttttgttcaa gttttgttga ttaccatata aaaatcatcg
    13441 gcttgaccta tcataacgct tcaaggcaaa tcatttgcaa tcttgcggca tcttcttatt
    13501 gcattttttt atggtaattt atatggtaaa ttttggacaa acgggaattt accataatgg
    13561 cgaaaatcat tacgccgctg tcggcaaatc aggttaaaaa tgcgaagccg cgcgataagc
    13621 tgtataagtt gtcggacggg ggggctggct ttgtgggtct acccgacggg cggacggagt
    13681 tggaagctgt cgtttatgca ggacggaagg caacagacaa tttcgctggg gcggtatcct
    13741 gatttctcgc tggctgaggc gcgggaatgg cgggaggagg tgcgccgaaa acgggcacac
    13801 ggggaaaatg tcgtcaataa gaaggtgcgg gcggattttg cttttgagaa ggtggcgcgt
    13861 gattggtttg tgcgttggtc gaaggggcgg tctgaaaagt atgccggaca ggttatgcgg
    13921 aattttgaac ggtgggtttt tccggctatc ggcaatcttg atatccgtca agtcaggacg
    13981 gcggacgtgg tcggctgcct gcgtgtgatg gaggcgcgcg gtatcgttga tacgttgcgc
    14041 aaaacgaaaa acagtctgaa gatggtgttt gcgtttgcgt ttgcggtcgg ttcgggaatg
    14101 atggaaatca accctgtcgc gcaaatcggt tcgggtgtgt ttgaacgggt gaaaaccaaa
    14161 aacatgacgg cgttgagtcc gtccgaattg ccgcgcctga ttgatttttt ggagcagcgc
    14221 aatgaatttg cggtttatgc gggcagggtg catatccatc ctgtaacgcg gttttgtatc
    14281 tattggctgc tgttgacgat gacgcggatt cgggaggcgg cgttgatgga gtggtcggag
    14341 ttggacgggg aggtttggcg tatccccgcc gaacggaaaa aggagcggcg ggggcatgat
    14401 gtgccgctgt cgcgggcgat gcggtgggtg ttggatcagg cgcgggggct gaatgtgaac
    14461 gggcggtttg tgtttgaaag tgtgaatttt caagggcata tcaataagga aagtccgcgc
    14521 gtggcgatgc ggcggtcggg gctggatacg acggcgcacg gtttgcgctc gcttgcgcgt
    14581 acttatttgc gcgaggttct gaaggtgtat aatgatgtgg cggaaaagct gcttgcccat
    14641 tcgttgggga cgaggacgca aacggcttac aaccgctcgg agctttggga ggagcgtaag
    14701 gatgcgccgg aacggtgggg gaatgatgtt ttgaggcttg ccgacaacgg aaaatgattt
    14761 ttttgtgtgt tttgcgttat gtggaaatgt agataagaaa gctgtttccg cattatgtga
    14821 aaacgcccta atcgggcgtt tttttattgc tgcaacccta atgcttccaa aacttcgcgg
    14881 gtgtcccata cgggggatgc gagggaggtt cagacggcac ggtatccgtc cgtctttttc
    14941 catacgcagc agtgtcgagt ttgaaatggg gtggttgcgg caggttgcgt aggcaatcag
    15001 ttcgcggatg gtcgggcggt ctatccttgc gcccggtttg gtgatgttca tttttttact
    15061 ctcctgttcg gtttaatctt gcgccgtttt tacggctctg tcgtttgtct gcctatttct
    15121 tcttcccgat ttttttatat cgcaacaagc tttccggcgg gaagaatatg tcaaagtctg
    15181 ttttcaaatc gggactgacg atgtcggcgg caatcacgcc gtagccggtg gcaaagctgt
    15241 cgccatcagc gttatgaata actatatgcc cgcttttgat tagaataacg cggccgaatc
    15301 cggatttccg gattccgcct tttttcgggg cggttgcaaa tgccagtgtt tccatccgcc
    15361 cttgtacgtc ggaagactcc attgcttcgc cgaccgcttt tttgacgggc gtttattgaa
    15421 tacttcgtat tccatgtctt caatcctctt tccgttgatt ccgatttgtc cgggtttccg
    15481 cttttttacg gcttggcatg cggcaattgc gtctttcata ttcggcatat cctttcccca
    15541 atccgccgaa gtttccgcat ccgccgccgt atcgattcat gtttgccctc cgatttgttt
    15601 tacgaagccc gtaccgttca cggcatagtg taacttcact tgcttacccg attccattcc
    15661 tcgggttaat gcctcatatt ccatttttga cccttgtagg attgacgatt tcgcatctct
    15721 tgtccttgct tcggccatat ccggatattc tttctttttg tatccgtcca taatggcttt
    15781 ttcttcatct gacatctcaa attttttgac tgtttcccaa gcgctcgcaa tccaaccgcc
    15841 gcaaaatcgg tcggcaagat aagttctgtg cgagggcttt cccgcccggc aggttttcaa
    15901 aaatttgcgg cggtcggcgg aaatctgacg atagactaca tcaaaggcat aggctgaggt
    15961 ttccgctcga tttccaatgc cgtaaaacat cattgttttc ccgcgttggt aagatttgca
    16021 cccgaatata tcggcaatca tatttgcaac gccccattgc cagcctgcca atttaaaagc
    16081 cattttccga tcggctttct gttcggagac ttttgataag gcgatatcct cggcatttac
    16141 tttgtacttt tccattaaag cctgcgcctg ccttaacgcc tgtgcggctt catgttcatt
    16201 tgccgaccga cccaaagcca aacatttctt gattttgtct aagactttct ctttatccat
    16261 attaaaccct ttaatttgtc cggtatttta atttcgcacc cttgcccatt cgtctttcgc
    16321 tttgctttcg attcggtcgg gccattcggc tagggctgat acgcttacga actcggggct
    16381 ttttttgttt ccgcctgccg agattaccgg ccgcggcagg gtagttttgc aacaaggctt
    16441 tgcaggcttc gggctgtatt tccacggcgt gaaattcaga cggcttaata taccgttcaa
    16501 gctgcctgct tcctgccgcg ccgtcaaata cgcccggatg ttcgccgcag tatcggcgga
    16561 ctttggcggc aa
//
LOCUS       DAXDXW010000055        16564 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXDXW010000055 DAXDXW010000000
VERSION     DAXDXW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16564
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.056"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            397..1239
                     /locus_tag="ACFB64_001485"
     CDS             397..1239
                     /locus_tag="ACFB64_001485"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC1209824.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLVDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGISLFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     DDRVAVISTLPLTRDESWSQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
     gene            1268..1732
                     /locus_tag="ACFB64_001486"
     CDS             1268..1732
                     /locus_tag="ACFB64_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC1209825.1"
                     /translation="MKCPFCSHPDTRVADSRLMEERNTVRRRRHCPGCGKRFGTLETV
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     ISSSELVEIVLEELFGQNTEAAVRFAALHKRFDSPEHFASWLAQAVKTGGKT"
     gene            1764..2873
                     /gene="ribD"
                     /locus_tag="ACFB64_001487"
     CDS             1764..2873
                     /gene="ribD"
                     /locus_tag="ACFB64_001487"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC1209826.1"
                     /translation="MFSDTNISMMENALRLAALGRFSTSPNPRVGCVIAHGRQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAKGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPPVAGKGLVLLKAAGIKTECGLLENKARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEEARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERDEDKLRPYREHAHIRILMPSETADGK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGGGKDLFSLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNGNAF"
     gene            3068..4489
                     /locus_tag="ACFB64_001488"
     CDS             3068..4489
                     /locus_tag="ACFB64_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC1209827.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLFSAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTTVYALANLAAAAFLLFQNRCRLKAVRRAPFSPAVLHRGLRYGIP
                     LALSSLAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLLQSIFSTVWTPYIFR
                     AIEENATPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFTVVSCMLPP
                     LFYTLTEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGTRGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAG
                     CILRHRKNLHKLFHYLKKQGFPL"
     gene            4657..5898
                     /locus_tag="ACFB64_001489"
     CDS             4657..5898
                     /locus_tag="ACFB64_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HFC1209828.1"
                     /translation="MSKAVKRLFDIIASASGLIVLSPVFLVLIYLIRKNLGSPVFFIR
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRTASLDELPELWNVLK
                     GEMSLVGPRPLLMQYLPLYNKFQNRRHEMKPGITGWAQVNGRNALSWDEKFSCDVWYT
                     DNFSFWLDMKILFLTVKKVLIKEGISAQGEATMPPFAGNRKLAVIGAGGHGKVVAELA
                     AALGTYGEIVFLDDRTQGSVNGFPVIGTTLLLENSLSPEQFDITVAVGNNRIRRQITE
                     KAAALGFKLPVLIHPDATVSPSATIGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTRIGEESRIGTGACSRQQTTVGSGVTAGAGAVIVCDIPDG
                     MTVAGNPAKPLTGKNPKTGTA"
     gene            6029..7204
                     /locus_tag="ACFB64_001490"
     CDS             6029..7204
                     /locus_tag="ACFB64_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC1209829.1"
                     /translation="MLNTALSPWPSFTQEEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALSNGTLALDAALKAIGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAVIVVHLAGMPAEMDGIMALAKEHDLWVIEDCAQA
                     HGATYKGKSVGSIGHVGAWSFCQDKIITTGGEGGMVTTNDKTLWEKMWAYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKHLPEWTARRQENAAKLAESLR
                     KFKSIRLIEVAGYIGHAQYKFYAFVKPEHLKDDWTRDRIVSELNARNVPCYQGGCSEV
                     YLEKAFDNTPWRPKERLKNAVELGGTALTFLVHPTLTDDEIAFCKKHIEAVLTEAAR"
     gene            7252..9162
                     /locus_tag="ACFB64_001491"
     CDS             7252..9162
                     /locus_tag="ACFB64_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFC1209830.1"
                     /translation="MNLETLIALPRNIKKICFLIHDFLMIFTAFWFTQSLKADYSDEW
                     FDFANWQSFLLTALLTITLFIRMGLYRAVTRFVSFHVLTTAFAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSDHQKKTHDPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPSATQEQ
                     RRRIISKLKAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMNAD
                     TEGKTVMVTGAGGSIGSELCRQIIRRRPERLLLFELSEFALYTVEKELCEYCARNGIA
                     AEILPFLGSVQNRTLLTHIMTAFSVTTVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVETFVLISTDKAVRPANTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGFVVPLFEKQIAEGGPITLTHPDITRYFMTIPEAAQLVIQAGAMGRGGDVFVL
                     DMGESVKITDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADETMLPWHELSALLDRIRTACDRYDQQAIRDLLVNAPTGFTPTGGICDL
                     LWVCETHRKNAV"
     gene            9218..10510
                     /locus_tag="ACFB64_001492"
     CDS             9218..10510
                     /locus_tag="ACFB64_001492"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC1209831.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFPNREYGWNLTVGNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGRHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAIYCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPPIIDNAYGMPFPNIIDSGVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEAVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKPYDNV"
     gene            complement(10562..11398)
                     /locus_tag="ACFB64_001493"
     CDS             complement(10562..11398)
                     /locus_tag="ACFB64_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC1209832.1"
                     /translation="MIDLHCHSTVSDGMLPPAEVVRLAHQNGCTLLALTDHDHTGGIS
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLADVRKGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIKNGHVKNKRQAFTKYLGDGK
                     SCAVRHEWATLADCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLADRFGLLASAGSDFHRLDDFSGGILGACPDLPENCKPVRAHFS
                     RH"
     gene            complement(11693..11896)
                     /locus_tag="ACFB64_001494"
     CDS             complement(11693..11896)
                     /locus_tag="ACFB64_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HFC1209833.1"
                     /translation="MDTTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRKKLKEAGWPDDAV"
     gene            complement(11874..12107)
                     /locus_tag="ACFB64_001495"
     CDS             complement(11874..12107)
                     /locus_tag="ACFB64_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC1209834.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKAFIAEGLVLRNG
                     GTEIRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            complement(12119..12355)
                     /locus_tag="ACFB64_001496"
     CDS             complement(12119..12355)
                     /locus_tag="ACFB64_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209835.1"
                     /translation="MPIAGVSGSPRLRMCISVNADSSGNRICSLPLGRISLDGWRHTG
                     FQTAFLRNRQCGHIAGFAAILGAAAAGATITPSF"
     gene            12640..16074
                     /gene="dnaE"
                     /locus_tag="ACFB64_001497"
     CDS             12640..16074
                     /gene="dnaE"
                     /locus_tag="ACFB64_001497"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC1209836.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPVGAADVWIGNPNAPDKPFRAMLVIRNDAGYLRLSELLT
                     EAYVGQDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDTFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDCLIKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLDDAMKAQPQIQELLEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTAGDIVDVGKIPLDDQTAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLGR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDSSGQAEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            16116..16370
                     /locus_tag="ACFB64_001498"
                     /pseudo
     CDS             16116..16370
                     /locus_tag="ACFB64_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3758 a         5164 c         4291 g         3351 t
ORIGIN      
        1 acagcaaccc tgtcgccgtc attaccgcga aagcgggaat ccagtccgtt cagtttcggt
       61 catttccgat aaattcctgt tgcttttcat ttctagattc ccactttcgt gggaatgacg
      121 gcggaagggt tttggttttt tccgataaat tcttgaggca ttgaaattcc agattcccgc
      181 ctgcgcggga atgacggctc aaaagttacg gaacgaaaaa caaccgaaac cggacaagtc
      241 ggattcccgc ctgcgcggga atgacggaat cttaagtttc cgtctttgtt ttctgttttc
      301 gcgggaataa tggggaacaa gccgtatttc agacggcatt ttcagttcgg ggtataatcc
      361 gaatacttgc gaccatctga atcatttgga caaactatgt gtcaactgct gggcatgaac
      421 tgcaatacgc cgaccgatat tatgttttcc tttgaaggct tccgccgcag gggcggcatt
      481 accgaccatc acgccgacgg tttcggcatc ggctttttcg aaggcaaagg cgtgcgcctg
      541 ttccatgacg acaagccgag tgcaaattcc cccgtcgccg acctcgtgcg cgcctaccag
      601 attaaatcgg aaaacgtcgt cgcccacatc cgcaaagcct cgcaaggtca aacctcgctg
      661 gcgaacaccc atccctttat gcgcgaaatg tggggcggct actggctgtt tgcccacaac
      721 gggcatttgg tcgatttttt ccccgaacag ggagaatttt tccaccccgt cggcacaacc
      781 gattccgaac gcgcgttctg ccacatcctc aaccgcctgc gcacccgctt tgccgcccgt
      841 cccgacgacg acacgctgtt tgacgcgatt gcggggctga cgcacgaaat ccgcaagttc
      901 gggctgttta acttcatgct ttcagacggc atttccctgt ttgcccacgc cagcacgctg
      961 ctgcactaca tcgtccgcca agccccgttc ggcaaagcgc gcctgctcga cgacgatgta
     1021 atggtcgatt ttgccgaagt aaccacgccg gacgaccgcg tcgccgttat ctccaccctg
     1081 ccgctgactc gcgatgaatc gtggtcccaa cttgccgtga acgaactggt catgttccgc
     1141 gaaggcaaca tcgtccggca cgaccgtccc gaaaatcccg tctatatgag tgccgaagaa
     1201 ggcctggaaa tcgcccgcgc cgccggcgta tcggtttgac gttcagacgg catcaggaag
     1261 aaacaccatg aaatgcccgt tttgcagcca cccagacacc cgagttgccg attcccgcct
     1321 gatggaagag cgcaataccg tccgccgccg ccgccactgc cccggctgcg gcaaacgctt
     1381 cggcacgctc gaaaccgtcg aactcaaaat gcccgccgtc atcggtccgg acaagaaacg
     1441 ttcgcccttc aatgcacaac gcctccgcaa cgacctgacc gccgccgccc gaaaatccgc
     1501 cctgacaccc gaacagattg acgaaaccgt ccgcctgacg gaacacaggc tctacacttc
     1561 gggtcagcgc gacatatcct catccgaact tgtcgaaatc gtgctggagg agctgttcgg
     1621 gcaaaacacg gaagccgccg tccgtttcgc cgccctgcac aaacgcttcg acagcccgga
     1681 acatttcgcc tcgtggctgg cgcaagccgt caaaacaggc ggcaaaacct gattccccca
     1741 acccatactg atacggtatc cctatgtttt cggacacaaa tatatccatg atggaaaacg
     1801 ccctccgact tgccgctttg gggcgttttt ccacttcacc caacccgcgc gtcggctgcg
     1861 ttatcgcaca cggcagacaa attgtcggac agggcttcca cgtcaaagcg ggcgaacccc
     1921 atgccgaagt ccacgccctg cgtcaggcgg gggaaatggc aaaaggcgcg accgcctttg
     1981 ttaccctcga accgtgcagc cattacggac gcacaccgcc ctgcgccgaa gccctgctcc
     2041 gttccggcgt aacacgcgtc gtcgccgcca tgcgcgaccc caacccgccg gttgcaggca
     2101 aagggcttgt cctgctcaaa gcagcgggca tcaagacgga atgcggacta cttgaaaaca
     2161 aagcacgcga actcaaccgg ggatttttat cgcgcatcga acgccgccgc ccctttgtcc
     2221 gcctcaaatg cgccgtttcg ctggacggca aaaccgccct ttcagacggc agcagctttt
     2281 ggattaccgg cgaggaagcg cgtgccgacg tacaggtttt gcgtgccgaa agctgcgcgg
     2341 tgctgaccgg catcggcacg gtgttggcag acaatccccg gctcaacgtc cgcgcttttc
     2401 caactttgcg ccaacccgca cgcatcgttt tagacagccg cctgcgcctg cccccgaaca
     2461 gccatttggt taccgacgga caatctccga cctacatcgc caccttggaa cgcgatgaag
     2521 acaaactccg cccctatcgg gaacacgcac acatccgcat cctgatgccg tctgaaacgg
     2581 cagacggcaa aatcgacctg caccatctga tgcgcctcct tgccgacgaa ggtttcggcg
     2641 aaatcatggt cgaagcaggc tccgaactca catccgcatt tttggcggaa aatttggcgg
     2701 acgaaatcgt gctgtaccgt tcgcccaaaa tcctcggcgg cggcaaagac ctgttttccc
     2761 tgcccgaaaa ccgtgccgcc ctttccgcac cgcccttgtg gacacccgtt tcaagcgaaa
     2821 tcctcggaca cgacatcaaa accgtgttcc ggaaaaacgg caacgccttt taaagggttt
     2881 gccgccgttt tgctatataa taacgccgat aaaaaacggc ccgttcagac cgccgcccgc
     2941 ccggaaaagc gcaacccgga ctgccgcacc ccgccggcag ccgcgacggc ctgaaagccg
     3001 tcaaattccg atcaagaaag gcttcagacg gcacaggcag catcacgccg ccgcccggac
     3061 atcaaaaatg gacacaaaag aaatcctcgg ctacgcggca ggctcgatcg gcagcgcggt
     3121 tttagccgtc atcatcctgc cgctgctgtc gtggtatttc cccgccgacg acatcgggcg
     3181 catcgtgctg atgcagacgg cggcgggact gacggtgtcg gtattgtgcc tcgggctgga
     3241 tcaggcatac gtccgcgaat actatgccgc cgccgacaaa gacactttgt tcaaaaccct
     3301 gttcctgccg ccgctgctgt tttccgccgc gatagccgcc ctgctgcttt cccgcccgtc
     3361 cctgccgtct gaaatcctgt tttcgctcga cgatgccgcc gccggcatcg ggctggtgct
     3421 gtttgaactg agcttcctgc ccatccgctt tctcttactg gttttgcgta tggaagggcg
     3481 cgcccttgcc ttttcgtccg cgcaactcgt gcccaaactc gccattctgc tgctgttgcc
     3541 gctgacggtc gggctgctgc actttccggc gaacaccgcc gtcctgacca ccgtttacgc
     3601 gctggcaaac cttgccgccg ccgccttttt gctgtttcaa aaccgatgcc gtctgaaggc
     3661 cgtccggcgc gcgccgtttt cgcccgccgt cctgcaccgg gggctgcgct acggcatacc
     3721 gctcgcactg agcagccttg cctattgggg gctggcatcc gccgaccgtt tgttcctgaa
     3781 aaaatatgcg ggcctggaac agctcggcgt ttattcgatg ggtatttcgt tcggcggggc
     3841 ggcattattg ctccaaagca tcttttcaac ggtctggaca ccgtatattt tccgtgcaat
     3901 cgaagaaaac gccacgcccg cccgcctctc ggcaacggca gaatccgccg ccgccctgct
     3961 tgcctccgcc ctctgcctga ccggaatttt ctcgcccctc gcctccctcc tactgccgga
     4021 aaactacgcc gccgtccggt ttaccgtcgt atcgtgcatg ttgccgccgc tgttttacac
     4081 gctgaccgaa atcagcggca tcggtttgaa cgtcgtccgc aaaacgcgtc cgatcgcgct
     4141 tgccaccttg ggcgcgctgg cggcaaacct gctgctgctg gggcttgccg taccgtccgg
     4201 cggcacgcgc ggcgcggcgg ttgcctgtgc cgcctcattc tggttgtttt ttgttttcaa
     4261 gacagaaagc tcctgccgcc tgtggcagcc gctcaaacgc ctgccgcttt atatgcacac
     4321 attgttctgc ctggcctcct cggcggccta cacctgcttc ggcacaccgg caaactaccc
     4381 cctgtttgcc ggcgtatggg cggcatatct ggcaggctgc atcctgcgcc accggaaaaa
     4441 tttgcacaaa ctgtttcatt atttgaaaaa acaaggtttc ccattatgaa aatcatcctg
     4501 actacttcta tgtcggttct gggaggcacg ggcacgacat catcgattgc caaatgtcgt
     4561 gcaaaaccga ccccgttcag tccgacgaaa tcgtccgccg cttcaggcgc gacatttcct
     4621 atcggaaaat cgtcaacctg attgaaagat tggcaaatga gtaaagccgt caaacgcctg
     4681 ttcgacatca tcgcatccgc atcggggctg attgtcctgt cgcccgtgtt tctggttttg
     4741 atatacctca tccgcaagaa tctgggttcg cccgtcttct tcattcggga acgccccgga
     4801 aaggacggaa aaccttttaa aatggtcaaa ttccgttcca tgcgcgacgc gcttgattca
     4861 gacggcattc cgctgcccga cagcgagcgg ctcacgccct tcggcaaaaa actgcgcacc
     4921 gccagtttgg acgaactgcc tgaactgtgg aacgtcctca aaggcgagat gagcctggtc
     4981 ggcccccgcc cgcttttgat gcagtatctg ccgctttaca acaaatttca aaaccgccgc
     5041 cacgaaatga aaccgggcat taccggctgg gcgcaggtca acgggcgcaa cgcgctttcg
     5101 tgggacgaaa agttctcctg cgatgtttgg tacaccgaca atttcagctt ttggctggat
     5161 atgaaaatcc tgtttctgac agtcaaaaaa gtcttgatta aagaaggcat ttcggcgcaa
     5221 ggggaagcca ccatgccccc tttcgcgggg aatcgcaaac tcgccgttat cggcgcgggc
     5281 ggacacggca aagtcgttgc cgagcttgcc gccgcactcg gcacatacgg cgaaatcgtt
     5341 tttctggacg accgcaccca aggcagcgtc aacggcttcc ccgtcatcgg cacgacgctg
     5401 ctgcttgaaa acagtttatc gcccgaacaa ttcgacatca ccgtcgccgt cggcaacaac
     5461 cgcatccgcc gccaaatcac cgaaaaagcc gccgcgctcg gcttcaaact gcccgttctg
     5521 attcatcccg acgcgaccgt ctcgccttct gcaacaatcg gacaaggcag cgtcgtaatg
     5581 gcgaaagccg tcgtacaggc cggcagcgta ttgaaagacg gcgtgattgt gaacactgcc
     5641 gccaccgtcg atcacgactg cctgcttgac gctttcgtcc acatcagccc gggcgcgcac
     5701 ctgtcgggca acacgcgtat cggcgaagaa agccggatag gcacgggcgc gtgcagccgc
     5761 cagcagacaa ccgtcggcag cggggttacc gccggtgcag gggcggttat cgtatgcgac
     5821 atcccggacg gcatgaccgt cgcgggcaac ccggcaaagc cccttacggg caaaaacccc
     5881 aagaccggga cggcataagc gattaaaata aacttccgta caaaccgatt ttgacaacac
     5941 ctgcggcgcg cgcccgattc ttcggaacac gcccccttca gacggcatag ggtcggaaat
     6001 gccgtccgaa cccgacggac caaacaccat gctgaacacc gccctttccc catggccgag
     6061 cttcacccaa gaagaagccg atgccgtttc caaagtcctg ctgtccaaca aagtcaacta
     6121 ctggacgggc agcgaatgcc gcgaatttga aaaagaattt gccgcctttg ccggcacgcg
     6181 gtacgccgtc gccctttcca acggcacgct tgcgctcgat gccgcactca aagcaatcgg
     6241 cataggcgcg ggcgacgatg tgattgttac ctcgcgcacc ttcctcgcct ccgcctcctg
     6301 cattgtgaac gcgggcgcaa accccgtgtt tgccgacgtg gatttgaaca gccaaaacat
     6361 cagcgcggaa accgtcaaag ccgtgctgac cccgaatacc aaagccgtca ttgtcgtcca
     6421 ccttgccggt atgcctgccg aaatggacgg catcatggct ttggcaaaag aacatgattt
     6481 gtgggtgatt gaagactgcg cccaagcgca cggcgcgacc tacaaaggca aatccgtcgg
     6541 ctctatcggg cacgtcggcg cgtggtcgtt ctgtcaagac aaaatcatca ccaccggcgg
     6601 cgagggcggt atggttacga ccaacgacaa aaccctgtgg gaaaaaatgt gggcgtacaa
     6661 agaccacggc aaaagctacg atgccgtgta ccaccgcgaa cacgcgcccg gtttccgctg
     6721 gctgcacgaa agtttcggca ccaactggcg catgatggaa atgcaggctg tcatcggccg
     6781 catccagctc aaacacctgc ccgaatggac ggcgcgccgc caagaaaacg ccgccaagct
     6841 ggcagaaagt ttgcgcaaat tcaaaagcat ccgcctgatt gaagtcgccg gctacatcgg
     6901 acacgcgcaa tacaagttct acgccttcgt caaacccgaa cacctgaaag acgactggac
     6961 gcgcgaccgc atcgtcagcg aactgaacgc gcgcaacgtc ccctgctatc aaggcggctg
     7021 ctccgaagtg tatttggaaa aagccttcga caacacgccg tggcggccca aagagcggct
     7081 gaaaaacgcc gtcgaactgg gcggcaccgc cctgacgttc ttggtgcacc cgacgctgac
     7141 cgacgacgaa atcgcgttct gcaaaaaaca catcgaagcc gtcctgaccg aagccgcacg
     7201 ataacccttc agacggcata tgccgcctga aagcacatac cgccccacga tatgaatctg
     7261 gaaaccctga tcgccctgcc gcgcaacatc aagaaaatct gtttcctcat acacgatttt
     7321 ctgatgattt tcaccgcctt ttggttcacc caaagcctga aggccgacta ctcggacgaa
     7381 tggttcgatt ttgccaactg gcagtctttt ttgctgactg ccttgctgac catcacatta
     7441 tttatccgaa tggggcttta ccgcgccgtt acacgctttg tcagtttcca cgtcctgacc
     7501 accgcctttg ccggcagcct cgcctccgcc gtattgttct tcctcaacac attgattttt
     7561 gaagaaaggc tgcgcctcgc cctgccgatt gtctatttct tactgctgtt tgtttccgtg
     7621 accggctcgc gtatggtttt gcgcggactc ctgtccgacc accagaaaaa aacacatgac
     7681 cccgtcatca tttacggcgc ggggcgttcg ggtcggcagc tccttgaagc cgtcaaacag
     7741 atgcgcgaat attccgccgc cgcctttgtg gacgacaacc ccaagctttg gcacaccgtc
     7801 atctacgacc ttgccgttta ccagcccgat gccatcgcct tcctcatcga acgctacggc
     7861 gtggaaaaaa tcctgctcgc cattcccagc gcgactcagg aacaacgccg ccgaatcatc
     7921 agcaaactga aagcctatcc gtgcgaagtg ttgaccattc ccggaatgaa agacctgatg
     7981 gacgggaaaa tcagcatcgg cacgctcaaa aaaatctctg tgtccgacct gctcgggcgc
     8041 gattccgtcg cacccgacga ccgcctcatg aatgccgaca ccgaaggcaa aaccgtcatg
     8101 gtaaccggcg cgggcggctc catcggttcg gaactctgcc gccagattat ccgccgccgc
     8161 cccgaaaggc tgctgctgtt cgagctgtcc gaattcgccc tgtacaccgt tgaaaaagaa
     8221 ttgtgcgaat actgcgcccg aaacggcatc gccgccgaaa tccttccctt tttgggttcg
     8281 gtgcaaaacc gcacgctgct cacgcacatt atgaccgcct tttccgttac gaccgtctat
     8341 cacgccgccg cctacaaaca cgtccctatg gtcgagttca acaccgtcga gggcatacgc
     8401 aacaacatct tcggcacact cgaatgcgcg cttgccgcca cggcttcggg cgtggaaacc
     8461 ttcgtcctca tctccaccga caaagccgtc cgccccgcca acacgatggg tgcgggcaaa
     8521 cgcatggcgg aactctgcct tcaggcactc gccgccgaac ccggacaaaa aacccgcttc
     8581 agcatggtac gtttcggcaa tgttttaggc tcgtccggct tcgtcgtccc gctgtttgaa
     8641 aaacagattg ccgaaggcgg ccccatcacc ctgacccacc ccgacatcac gcgctatttc
     8701 atgaccatac ccgaagcggc gcaactggtt atccaagccg gcgcgatggg cagaggcggc
     8761 gacgtgttcg tcctcgatat gggcgaatcc gtcaaaatca ccgaccttgc ccgccaaatg
     8821 attaccttaa gcggactcaa acccaaaacg cccgaacagc ccgacggcga catcgaaatc
     8881 ctcattaccg ggctgcgccc cggcgaaaaa ctctacgaag agttgctcat cggcgacaac
     8941 gtccgcaaaa ccggccatcc gcgcatcatg accgccgacg agaccatgct gccgtggcac
     9001 gaactctccg ccctgctcga ccgcatccgc accgcctgcg accgttacga ccagcaggca
     9061 atccgcgacc tgctcgtcaa cgccccgacc ggctttaccc cgaccggcgg catctgcgac
     9121 ctgctttggg tatgcgaaac acacagaaaa aatgccgtct gaaccttcag acggcatacc
     9181 gtacaaacca atctgcctta cacacactga gtttgacatg cagttctcgg cattcggcga
     9241 aaaattcacg caacacagcg gcatcctgca actgatggac gacctcggcg acgcgctcaa
     9301 aagcgacaag cccgtcaata tgctcggcgg cggcaacccg gcgcgcattc ccgaaatcga
     9361 tcgggcgttc gccgacatat tttccaagct ggcggcggaa cacgccgtcg agaacatcgg
     9421 caactactcc aatccccaag gcgatgccgc gctgattgac gcgctgaccg ccttccccaa
     9481 ccgcgaatac ggctggaacc tgaccgtcgg caatatcgcg ctgaccaacg gttcgcaaaa
     9541 cgcgtttttc tatttattca acctcttcgg cggcaaattc aagctttcag acggcacatc
     9601 cgcagaaaaa gccattttgt tgccgctcgc gcccgaatac atcggctatg ccgacgtgca
     9661 tgtcgaaggg cggcacttcg tttccgtcaa acccaaaatc gaaaacgtcg aacacgaagg
     9721 cgaagcaggc ttcttcaaat accgcgtgga ctttgacgca ctggaaaacc tgcccgaact
     9781 caaagcgggc aaaatcggcg cgatttactg ttcgcgcccg accaacccga ccggcaacgt
     9841 gttgaccgac ggcgaaatgg cgcgtttgga cgctttggcg cgtgaacacg gcattccgcc
     9901 gattatcgac aacgcctacg gaatgccgtt ccccaacatt attgacagcg gcgtaacgct
     9961 gaattggcac gaaaacatca tcctctgctt cagcctttcc aaagtcggcc tgccgggcgt
    10021 tcgcaccggc atcatcgtcg ccgcgcccga agccgtcaaa gccgtcagca gcctgaacgc
    10081 gattgtgaac cttgccccca cgcgcttcgg cgcggccatc gccgccccgc tgctggaaag
    10141 cggcgagatg aaacggcttg ccgaccaagt catccggccg ttttaccgca atcaggcgca
    10201 aaccgccgtt tcgctgctca agcgcgagct gggcgcgtac ccgatgaaaa tccacaaacc
    10261 cgaaggcgcg attttcctgt ggctctggtt tgaaaacctg cccgtttcgt cgcaaaccct
    10321 gtacgaaatg ctcaaagccg aaggtacgct gattattccg ggcgaacatt tcttcgtcgg
    10381 catcgacacg caagactacc cgcacgcggg cgagtgcatc cgtatgagta tcgcacagga
    10441 cgctcaaacg ctggaaaaag gcatcgccgc catcggcaaa accgtccgaa aaccgtacga
    10501 caacgtttaa aacgcaaaaa atgccgtctg aagacctttc agacggcatt ttcatccgca
    10561 ttcaatgtcg ggaaaaatgc gcccgcaccg gtttgcagtt ttccggaaga tcgggacacg
    10621 cgccgaggat gccgccgctg aagtcgtcta aacggtggaa gtcgctgccc gcgcttgcca
    10681 acaatccgaa gcggtctgcc aaaagcgcgt agttgaggcg gtcgtttttg cagcagttgc
    10741 cgctgtggac ttcgatgccc gcgccgccga ggtttttaaa ttcttcaaac aggttgcgct
    10801 tggcggttgc cgacaaatca taacgcatcg gatgggcaat caccgccatg ccgcccgcgc
    10861 cgttgacggc ggagacgcag tctgccagcg tcgcccattc gtggcgtacc gcgcaggatt
    10921 tgccgtcgcc caagtatttg gtgaacgcct gccgcttgtt tttgacgtgt ccgtttttga
    10981 tgaggaactc ggcgacgtgg gtgcggctga ccatttcttt gtttgccgcc agcgccagcg
    11041 cgccgtcgta tgcgccgccg atgcctttct tttcgagctt ggcggcgatg gcttcaagac
    11101 gtttcagacg gcctttgcgg acatctgcca acaggttttg caggttttcg tcctgctcgt
    11161 cgaaatccaa accgacaacg tgtatggtac gcccgcgcca cgttaccgaa atttccacgc
    11221 cgttaatcag gcgcaaaccg agcctgtcgg cttcggcacg cgcttcggag atgccgcccg
    11281 tgtggtcgtg gtcggtcaac gccagcagcg tgcagccgtt ttgatgtgcg aggcggacga
    11341 cttcggcggg ggggagcata ccgtcggaaa cggtggaatg gcagtgcagg tctatcatgg
    11401 ggtgttatgt ggtggtgaat gaaggacggg atgtttcaat gttgtcggac agtgtggcgg
    11461 cggcggtcat tttaaaaaac aggtgtatgg caaatgcaaa ggaaaagccg ctatgccgtc
    11521 attcccgcgc aggcgagaat ccagactttg atttattgga aatgtttaag gttaaccgct
    11581 atttcaaact tccggattcc tgctttcgag ggaatggcga tatgaacgtt ttcaatttta
    11641 atctactata aaagaccgtc tgaaaacgtg gttttatcaa gcccaaccgt tttcagacgg
    11701 catcgtccgg ccaacccgct tccttcagtt ttttccgttg cgcatcgtag cgtgccttgt
    11761 ccgcccagta aatcgtctga atgcacgcct cgccgcagtc gctgccgcag cattcccacg
    11821 actcggggcg gacgggttcg tccaaaagcg gctcgcccaa aagggcttcg gctttatgct
    11881 tcagggtcgt atccatcggc gatttccaag cgcgcgccgt caaactcgat gacttctccg
    11941 ccgcgtattt tggccgtttt gcggatttcg gttccgccgt tgcgcaacac cagcccttcg
    12001 gcgataaatg cttttgcctg tccgccgctt tcggcaagtc cggccaattt caagaggtcg
    12061 cacaaagcga tgtattcgtt gtcttcgaga tagacggtgg cttccataat gtcccctttt
    12121 agaaagaggg cgttattgta gcacctgccg ccgccgcacc caaaattgcc gcaaaaccgg
    12181 cgatgtgtcc gcactgcctg ttccgtaaaa atgccgtctg aaaccccgta tgccgccatc
    12241 cgtccaaaga aatcctgccc aacggcaggc tgcaaatcct gttccccgac gaatccgcat
    12301 tgacgctgat gcacatcctc aaacgcggac tgcccgatac accggcaatc ggcatcaaaa
    12361 cgaaatcaaa aacctgtttg aacgtaaaat ctatttaact gcttgccgtc cgtccgaata
    12421 aggtatatag ttctttataa ctagtttgat agtcctttat atctatcaat actccttggg
    12481 aagcctccgc cacacggcag gaggcatttt tttgccgtcg taaaagctca aaaaacattt
    12541 gcaggtcatg ccgtctgaat ccgaaacggc attacctgca ccgccatcta aagacaaccc
    12601 tgctacaata cgccttttat tgtccacgcc gattttgcca tgaccgagcc gacctacatc
    12661 cccctgcgcc tgcataccga attctccatt accgacggta tggtgcggat taaaaaactg
    12721 attgccaaag cgcaagaata cggtttgcct gctttgggca tcagcgattt gatgaacgaa
    12781 ttcggtttgg tgaaattcta taaagcctgc cgcggcgcag ggattaagcc cgtcggcgcg
    12841 gcggacgtgt ggataggcaa tccgaatgcg cccgacaagc cgttccgcgc catgctggtt
    12901 atccgcaacg atgcgggtta tctgcgcttg agcgagcttt tgacggaggc ttatgtcggc
    12961 caagaccgca atatccatca tgcggaactc aatcccgaat ggctggaaaa cggcgacaac
    13021 agcggcttga tttgtttgag cggcgcgcat tacggcgaag tgggcgtgaa tctgttgaac
    13081 ggcaatgaag acgcggcgcg tgcggcggcg ttgaaatatg cggcatggtt tcccgatacg
    13141 ttttatttag agctgcaacg cctgcccgaa cgccccgaat gggaggcttg cgtttccggc
    13201 agcgtgaagc tggcggagga actgggtttg ccggtggtag caacgcatcc gacacagttt
    13261 atgaaccgcg acgatttcaa cgcgcacgag gcgcgggtgt gcatcgcggg cggctgggtg
    13321 ctgacggaca agaaacgtcc gcgcgatttc acgccgagcc agtttttcat tccgccggaa
    13381 acgatgctgg agcgtttcgc cgacttgccc gaagccttgg aaaacacggt ggaaatcgcc
    13441 aaacgctgca acttgcacat cacattgggc aaaaacttcc tacccctttt ccccacgccc
    13501 gacggtttat cactcgatga ctgtctcatc aaactctcca acgagggttt gcaggaacgc
    13561 atggttcagc tttatcccga cgaggcggaa cgggcggcaa aaatgccgga atatcaggag
    13621 cggctggact ttgagctgaa catcatcatc caaatgaaat tccccggcta tttccttatc
    13681 gtacaagact ttatcaactg ggcgaaaacg cacggctgtc cggtcgggcc gggacgcggt
    13741 tcgggcgcgg gttcgctggt ggcgtattcg ctgaagatta ccgacctcga cccgctgaaa
    13801 tacgcgctgc tgttcgagcg tttcctaaac cccgaacgcg tttccatgcc cgactttgac
    13861 gtggacttct gtcaggcaaa ccgcggccgc gtgattgaat acgtgcgcga aaaatacggc
    13921 gctcaggcgg tcagccagat tgtgactttc ggcacgatgt cgtccaaagc ggtcatccgc
    13981 gacgtggggc gtgtgctgga actgccgttt atgctgtgcg acaaactgtc caagctgatt
    14041 ccgttggaag ccaacaaacc cctgagtttg gacgacgcga tgaaggcgca gccgcagatt
    14101 caggaattgc ttgaagcgga agaagcggac gaactgatta cgctggcgaa aaaactggaa
    14161 gacctgacgc gcggtttggg tatgcacgca ggcggcgtgt tgattgcgcc gggcaagatt
    14221 tccgattaca gccccgtgta tcaggcggac gaatccgcct cgcccgtatc catgtacgac
    14281 aagggcgacg tggaagacgt gggtttggtg aagttcgact ttttgggcct gcgcaacctg
    14341 accattatcg aaatggcgca gaacaacatc aaaaacaccg ccggcgatat cgtcgatgtc
    14401 ggcaagattc cgcttgacga ccagactgcc taccaaatct tccgcgatgc gaacaccacc
    14461 gccgtcttcc agttcgagtc gaccggcatg aaaaaaatgc tgaaaacggc gcatacgacc
    14521 aagtttgaag aactcatcgc cttcgtatcg ctctaccgac ccggcccgat ggacaacatt
    14581 cccgacttcg tcgcacgcat gaagggacag gaattccaat acatccaccc gctgctggaa
    14641 ggcatcctcg cgccgaccta cggcattatg gtgtatcagg aacaagtgat gcaggcggcg
    14701 cagattatcg gcggctactc gctcggcggc gcggacctgc tgcgccgcgc catgggtaag
    14761 aaaaagcccg aagagatggt gaaacaccgc gaaatcttcg ccgaaggtgc ggcaaaacaa
    14821 ggcatttcgc gcgaaaaatc cgacgaaatc ttcaactaca tggaaaaatt cgccggctac
    14881 ggtttcaaca aatcccacgc cgccgcctac gccctgattt cctaccagac cgcatggctc
    14941 aaagcccact atcccgccga atttatggcg gcgacgatgt cgtccgaatt ggacaacacc
    15001 gaccagctca aacatttcta cgacgactgc cgcgccaacg gcatcgagtt cctgccgccc
    15061 gacattaacg aatccgacta ccgcttcacg ccgtatccga acatgaaaat ccgctacgca
    15121 ctcggcgcga ttaaaggcac gggcgaagcc gccgtcgaat ccatcatcgc cgcgcggcaa
    15181 agcggcggca aatttaccgg cctgttggac ttctgcgagc gcgtcggcaa agaacacatg
    15241 aaccgccgca ccctcgaagc cctgatacgc ggcggcgcgt tcgacagcat cgaacccaac
    15301 cgcgccatgc tcttggcgaa catcgacctt gccatggaca acgccgacca aaaagccgcc
    15361 aacgccaatc agggcgggct gtttgacatg atggaagacg ccatcgaacc ggtgcggctc
    15421 atcgacgcac ccatgtggag cgaatcggaa aaactcgccg aagaaaaaac cgtcatcggc
    15481 ttttacctgt ccggccaccc gttcggcccg tatgcccaag aagtccgcca aatcgccccg
    15541 accaaattag gccgtctgaa accgcaagac agcgtgcgcc tcgccggatt cgttaccgcc
    15601 gtgcgcacca tgatgggcaa acgcggcaaa atcgccttcg tcagcctcga agattcgagc
    15661 gggcaggctg aaatcatggt cggcggacaa acgctggaaa actgcgccga ctgcctcaaa
    15721 gccgaccaag tgctgattat cgaatccaaa gtcagccgcg acgattacgg cggcggcgac
    15781 gggctgcgca tcatggcaaa ccaagtcatg accctgcaaa cggcgcgcga acgctacgcc
    15841 cgcagcctca gcctcgccct tgccccgcat cacgacatcg gcggactggt acggctgctc
    15901 gccgcccacc aactgcccga cacgccgcgc atcccgcttc aattgtcgta tgccaacgaa
    15961 aaagcgtcgg gcaggcttca agtgcctccg aaatggacgg ttacaccgag ttccgcattg
    16021 ttcggcgaac tggaaacatt gctcggcagc cggtcggtgc gcgtgaattg gtaaccaaaa
    16081 atacaaatgc cgtctgaagc ccaaaaccgg tttcattcgt actttattcg aatgattgaa
    16141 taaaagtaac tgccaagaaa aacgtatttt ttggttattt cgccagccta aataaatcaa
    16201 ccggggcgat tgatatccgt gtacatgaca cagacagcac caaagggaaa aacggcattt
    16261 tcaaaagtat cggtgtcaaa accgcccttt cattccaaaa ataccaaatc gacgaaccgg
    16321 gcaaagaaat cagaccgtgc cgcctgaaaa aacgcccgcc cgtccgttaa attaatctat
    16381 ccctgtttca ggcggcctga agcagggatt tttatatcaa gataaaatga gaacgggagc
    16441 gacaaccacg ggcaaagtcg gcgcgaacta tcaaaaattg tagcactgcg aaatgaggac
    16501 gggagcaata acccttaggt agctcttgtt atagcggatt aacgaaaatc aggacaaggc
    16561 gacg
//
LOCUS       DAXDXW010000056        16520 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXDXW010000056 DAXDXW010000000
VERSION     DAXDXW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16520
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.057"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            109..2136
                     /locus_tag="ACFB64_001499"
     CDS             109..2136
                     /locus_tag="ACFB64_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFC1209837.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSGLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITVGAPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFGVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSLKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLLKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVRIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(2225..3778)
                     /locus_tag="ACFB64_001500"
     CDS             complement(2225..3778)
                     /locus_tag="ACFB64_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC1209838.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGETAEGDAFFENVRRFPEKPDL
                     DRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGEDEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            3829..3957
                     /locus_tag="ACFB64_001501"
     CDS             3829..3957
                     /locus_tag="ACFB64_001501"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209839.1"
                     /translation="MPSENGRRQFIIFAFRRHIKRRLCAACRRFAPVLPMSDLNAV"
     gene            complement(4029..5222)
                     /locus_tag="ACFB64_001502"
     CDS             complement(4029..5222)
                     /locus_tag="ACFB64_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC1209840.1"
                     /translation="MNPKHFIAFSALFAATQAEALPVASVSPDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDALRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHSGNWQSFTSRNNTGDFPLGGILAQTGSGGGMTLTASDG
                     KTGKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLAIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVLLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAQGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(5219..6202)
                     /gene="patB"
                     /locus_tag="ACFB64_001503"
     CDS             complement(5219..6202)
                     /gene="patB"
                     /locus_tag="ACFB64_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC1209841.1"
                     /translation="MKNFLSLFTSILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYALSDGIKTFLSGETPPTAQDGGSADMPPEAAASEAAPPAGGT
                     EWKQDTEAAAIRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESANLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKVKLDGQMRYLDKLLSEHLKGKIILIPTAQTLSGGKGR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAEKIMEKIVFEPSTQPSSTQP"
     gene            complement(6213..7649)
                     /locus_tag="ACFB64_001504"
     CDS             complement(6213..7649)
                     /locus_tag="ACFB64_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC1209842.1"
                     /translation="MPLLSVEFALFFIVFLPIYWGFAKYPSVQNLLLLAAGMGWLYHI
                     SPVFAAIIVLYSSCVYLLGELLRSDRESTRRFWLGCGIAASITVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSVAYLVYCFRAPHAARFGWHELLLHLSFFPTVTS
                     GPIIRAAAFKSTDGEQAGALAQIRTRRPRSPVRPALAVSLILLGIAKKWWLAGILAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALALLNTGDRYFGRDALCRLKYLAPLSWLITFHFVCLTFVVFNTANPD
                     DAGAVFSALFANAGGWNAPQRADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            complement(7779..8944)
                     /locus_tag="ACFB64_001505"
                     /pseudo
     CDS             complement(7779..8944)
                     /locus_tag="ACFB64_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
     gene            9385..9597
                     /locus_tag="ACFB64_001506"
     CDS             9385..9597
                     /locus_tag="ACFB64_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC1209843.1"
                     /translation="METLLLDIGGMSCGGCVKSVTRILESVKGVASVEVSLENKSATV
                     GYDPAQTAAEALIEAVEDGGYDAALK"
     gene            complement(9576..9737)
                     /locus_tag="ACFB64_001507"
     CDS             complement(9576..9737)
                     /locus_tag="ACFB64_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209844.1"
                     /translation="MSEGTIARKFNQFILPVQLNQMPPEGAERASDGIGETQRLQTAF
                     LPLYFNAAS"
     gene            9745..11324
                     /locus_tag="ACFB64_001508"
                     /pseudo
     CDS             9745..11324
                     /locus_tag="ACFB64_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
     gene            11406..12503
                     /locus_tag="ACFB64_001509"
     CDS             11406..12503
                     /locus_tag="ACFB64_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC1209845.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYVLGFGSGKVQAALGQNLLQAGLPLMQ
                     VWQENLASEGITLFANPLSPDTPARALSDGSHTRQRMAMDVFAANAIRAVRMQGPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFCWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQENIPSYAEALKREGRNPFFSA"
     gene            12617..13731
                     /locus_tag="ACFB64_001510"
                     /pseudo
     CDS             12617..13731
                     /locus_tag="ACFB64_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            13799..14311
                     /locus_tag="ACFB64_001511"
     CDS             13799..14311
                     /locus_tag="ACFB64_001511"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC1209846.1"
                     /translation="MPSERVSASDGIALEQGWDMKKPKILFVCLGNICRSPMAEYILR
                     RRAAEAGIPLETDSAGTSGWHDGEDMHRETAKILKKYGIDASGFTSRKIRQSDATAFD
                     YIIAMDGKNLSELEKTFGRRPEKIFKLTDLIPESGYDHVPDPWYMGDFEETYRLADAG
                     CRALLEKISK"
     gene            14388..14864
                     /locus_tag="ACFB64_001512"
     CDS             14388..14864
                     /locus_tag="ACFB64_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC1209847.1"
                     /translation="MKTNFKQKIIEQARREDLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWADQGVLHKVAAVNGYILCSHAQHECNDHCHDHEEA
                     EAHHSAFILVCTECGAADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            14852..15778
                     /locus_tag="ACFB64_001513"
     CDS             14852..15778
                     /locus_tag="ACFB64_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC1209848.1"
                     /translation="MSEVKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLAAGKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVVEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            15843..16400
                     /locus_tag="ACFB64_001514"
     CDS             15843..16400
                     /locus_tag="ACFB64_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC1209849.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATRVLMETDMGNIRLVLDESKA
                     SKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLVQKATDKAVANESGNGLKN
                     TVGTIAMARTADPDSAAAQFFINLADNGSLDYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATRGLYQNVPVQPVKIRRVVVGQ"
BASE COUNT         3875 a         4173 c         4666 g         3806 t
ORIGIN      
        1 ggccggtttg tgcatttcgt catccggaag ccggtatttt gccggcaggt tttgactact
       61 ttgcccgatg accgggtttg tctgttttta acaaaggagt gagaaggttt gtccctgtct
      121 gaatttatag aacgccgaac gtcatttaat ccgatggtta ttttgacgac tttgtttttt
      181 gtgtgtgttt tggtggtatt ggttttaacc gtgccggatc aggtgcagat gtggctcgac
      241 cgggcaaaag aagtcatttt taccgagttc agctggtttt atgttttaac gttttccatt
      301 tttctgggtt ttctgctgat actctcggtc agcggtttgg gaaacatcag gctaggacgg
      361 gatgaagatg tgccggaatt cggcttcctg tcgtggctgg cgatgctgtt tgcggccggg
      421 atgggcgtgg gcctgatgtt tttcggcgtg gcagagccgt tgatgcatta tttttcggac
      481 attacggtcg gcgcgccgga acacaggcag cagcaggcat tgctgcacac ggtgttccat
      541 tggggcgttc acgcctggtc ggtgtacggt acgattgcat tggctttggc ttatttcggt
      601 ttccgctaca aactgccgct tgccctgcgt tcttgttttt accccctgtt gaaagaaaaa
      661 atttccggaa ggttcggcga tgccattgat attatggcgt tgcttgctac ttttttcggc
      721 atcatcacca cattggggtt cggggcttcg caactgggcg ccggattgca ggaaatgggc
      781 tggattgccg aaaacagctt cggcgtgcag gtcttgatta tcgccgccgt aatgtccctc
      841 gccgtcgttt cggcaatatc cggcgtgggg aagggcgtga aggtgttgag cgagttgaac
      901 ctgggccttg cgtttttgct gctgtttttt gttttggcgg cgggccccac tgtttacctg
      961 ttgtcggcat tcggcgacaa catagggaac tacctcggaa atctggtgcg cctcagtttg
     1021 aaaacttatg cgtacgaacg ggaacacaag ccgtggtttg aatcttggac ggtgctttat
     1081 tgggcgtggt ggtgttcttg ggcgccgttt gtgggtttgt ttatcgcgcg catttcaaag
     1141 gggcgcacca tccgcgagtt tgtcttcggg gttttgctca tccccggcct gttcggcgtt
     1201 ttgtggttta ccgtcttcgg caatacggcg atttggctga atgacggggt tgcgggggga
     1261 atgctcgaaa agatgacctc ctctccggaa acgctgcttc ttaaattctt taattacctc
     1321 cccctgcccg aactgacgag catcgtcagc ctgctggtca tttccctgtt ttttgtaact
     1381 tctgccgact ccgggattta tgtcctgaac aatattacct ctcgggacaa aggcttgagc
     1441 gcgccacggt ggcaggcggt tatgtggggc gtgctgatgt ctgccgttgc cgttttgctg
     1501 atgcgctcgg gcggactcgg caacctgcag tctatgaccc tgattgtttc cctgccgttt
     1561 gccctgctga tgctgataat gtgtttcagc ctgtggaaag gcttgagtgc ggataagaaa
     1621 tattttgaga cccgggtcaa ccctaccagt gtattttgga cgggcggcaa gtggaaagaa
     1681 cggctggtgc ggataatgag ccagacgcag gagcaggata ttttaaaatt cctcaaacat
     1741 accgcatcgc ccgctatgca cgagttgcaa cgggagcttt cggaagaata cggcttgagc
     1801 gtccgggtcg ataagatgtt tcatcaggac gagcccgcaa tcgagttcgt cattcggaaa
     1861 gagacgatgc gcgattttat gtacgggatt aagtctgtcg ggcaggatgt atccgaccag
     1921 ttgattaacg acggcaagct gccgcatatc cggcaccaga caacttacaa accctacgct
     1981 tattttttcg acgggcgcgt cgggtacgat gtgcagtata tgaacaagga cgagctgatt
     2041 gccgacattt tgaaaaacta cgaacgttat ttgatgttgt tggatgatgt cggtcaggaa
     2101 ctgatggcgc acgagcaggt ggaattggca gagtaagcgc cttccctccg gcgtttgcca
     2161 aaaaatgcgg tgaatcagat tcaccgcatt tttttgccgg acggggcttc ggacggcacg
     2221 gcgttcattt gtttccgtca aactgctcct tcagcacccg tttcaatact ttgcccgtag
     2281 cgttgcgcgg cagcccgtct ttaaagtgga tctgtttggg gattttgaaa tttgccagca
     2341 cggtacgcag gtggcggcgg atttcgtcct cgcccaaatc cataccttcc ttcaattgga
     2401 cgaaggcgac gatttcctcg tcggcataac ggtctttcac gccgatgacg gcggcggctt
     2461 cgacggcatc gagtttgtag atttcttctt cgatctcgcg cggatagacg ttttgacctt
     2521 tggaaataat caaatctttt ttgcggtcga cgataaagat aaagccgtcc tcgtctatgg
     2581 taacgaaatc gcccgttttc aaccagccgt tgacgatggt ttcatcggtg gcggcaggca
     2641 tattgaggta gccccgcatc accgaaccgc ccctgacgat cagttcgccc acttcgccgc
     2701 gcggcacttc gaccaattct tcatcgacgg ctttggcttc caaaccgggc agggggatgc
     2761 cgacgctgcg ggctttttgc cgttcgggcg tattgacggc gacgacgggc gaggcttcgc
     2821 tcagtccgta gccttccagc aatttggcgc gggggaactt ggctttaaaa tcgaggatgg
     2881 tttgttccgc caaaggcgcg ccgccgctga taaacaggcg gatgcggttg aaccatctga
     2941 aataccaagg gattttcgcc ttgctcatcg cggtgtaaat cgcgggtacg cccaaaaaca
     3001 cggttgcgcg tttgagcagg gcctgtttca aaacgttgga aaaggggaaa acggatttga
     3061 ccaaaataat cgaacacgcc atataaatcg gcagcagtac catagccgtc agcgtgaagc
     3121 tgtggaacat cggcaggaaa acgataaagc ggtcgcgttt ggaaatttta aagatgcgtt
     3181 cgatgccgtt caggttggcg aacaggttgg cgtaactgat tagcgcgcct ttgggatgcc
     3241 ccgtcgtgcc ggaggtgtag atgatgtgtg ccaaatcatt tatccggggt tggcggtcca
     3301 agtcgggttt ttcggggaag cggcgcacgt tttcaaaaaa ggcatcgcct tccgccgttt
     3361 cgccggccgg ccggcttttg tccgtccaaa tgattttttc gacgggcgtt tgcgccttca
     3421 ggcccgccaa ttcttttgac aggccggccg aggcgaacag gaagcgcgcc ttgcagtcat
     3481 tcaggatata cgcgtattcg ctgtttttca aaaatgtgtt catcggtacg gcgaccgcgc
     3541 cgatggcgga gacggcgaaa taggcggtaa taaattctgt ggaattggaa accgccagcg
     3601 cgaccgtgtc gccgaacttc acgccgatat tttgcagata cgccgcgacg gcttcggctt
     3661 cctgcttgag cgcgcggtag gcggtttttt ccttgccgtc gaacactgcc gtgccgttgc
     3721 cgtttttgcg gcaggcggcg gcgagcattt cgtagaaatt ggcataagtc cggttcatga
     3781 aacttccttt atggttgcgg acggcgaacg ccctcatttg aaaacaatat gccgtctgaa
     3841 aacggaaggc ggcagtttat catctttgcc ttcagacggc atatcaaacg ccgcctttgc
     3901 gccgcatgcc ggaggttcgc ccccgtcctt cctatgtctg acctgaatgc cgtctgaatc
     3961 aatatgaaga tatacgtttc agacggcatc cccctgttgt ggaaataccg tccgcccccc
     4021 ctgtccgatt attgcctgat tgcggcggcg cggacgagtt cttcgaggct gtcggcaagc
     4081 atttccgccg cgcgccggta gccttgggcg gagaagtgta cgccgtcttt ggcggcccat
     4141 ccttggttga gccagttttt catgctgcat atgccgccca ttgcgttttg ccaagaccaa
     4201 aacatcgtct gcccctgacg ggcgacgcgc cgctgcatct gttggacttc ggtcaggagg
     4261 acggggcgcg tgccgcatac gccgagcgtg tttttcaggg attcgggcgc gccgatgatg
     4321 aggatgccgg cggcgggcag gctgtcgcgg atttggcgga cggtatccag ccatttttgt
     4381 tcggtatcgg caatgtcgat gttgttgttg aaggcttcgt tggtgccgta ggaaaggata
     4441 accaaatcgg cgccggtttg ggcaaggtcg ttcatacggt cggcacgcca tttcgaccac
     4501 tgggtcaatt gtgcgccgtt gatgcccatc gcggaaacgg taatgccgcc ggcgggattt
     4561 tcgatgttga tgaagccgat gtcccacggc atttcggtct gtatggccag gggcagtgcc
     4621 gcgcccgtat ccagtacctg ccagccgccg ccgttggcgg agacggtgtt gccgttgacg
     4681 gtcagggttt gttcggcgag cagcggtttg gcaaacaggg aaacgcgctg tttgccggtt
     4741 ttgccgtcag acgcggtcag ggtcatgccg ccgccgctgc cggtttgggc gaggatgccg
     4801 ccgagcggga aatctccggt attgttcctg ctggtgaagc tttgccagtt gccgctgtga
     4861 cggacggccg ccatgcgctg ccctttgacg ttggcggggt aaacccagcc tatgccgccg
     4921 tcgccccatg ttttttgcag gcgtttgcgc agggcgtcgg taaagaagtc gccggcggta
     4981 tgcgagtcgc cgatttgcag gatgcggaag gtctcgccgc tgccttgtgc gacggatcgg
     5041 agttttttca tccaaggcga ggcggcggcg ttgccgtagt cggtcagcag cccgtttgta
     5101 tcggtgtagg gggcggacgg ggaaacggta acggtgtcgg ggctgacgga ggcgacgggc
     5161 agggcttctg cctgcgtggc ggcgaacagg gcggaaaatg cgatgaagtg tttggggttc
     5221 atggctgtgt acttgatggt tgcgtactcg gttcaaaaac gattttttcc attatttttt
     5281 ccgccagcag tttttgtcct tcggcggtaa agtgtatgcc gtccttactg cggtagcgga
     5341 cgggtttgcc gttgacgttg acggaatcgg tgtagcggcc tttcccgccg ctcagtgttt
     5401 gcgcggtggg aatcaggatg attttgcctt tcaagtgttc cgaaagcagt ttgtcgaggt
     5461 agcgcatctg accgtcgagc ttgacttttt tcatgtaggg gatgccgagc cagacgactt
     5521 ggacgcggtg cgtgtgtgcg gcttcaagga tgcggtcgac gcgtttcagg tattcttgcg
     5581 cccattcgtc ggaagcgaat ttgaggtagc gtttgccgac ggggaaatcc cacgggtcgt
     5641 tcgggccgag gaagacggcg agtacgctga tttcgggatg ttttttcaag gtttcttcaa
     5701 tcgttttcgg ccagtcgaag aatgagggat aggaaagccc cgtgctttgt ttgctgaggt
     5761 tggcggattc gatgccgtat tgctgtttca ggcttttttg cacgaagggc gcaacgccct
     5821 gcatcagcga atctccggcg aaaaagactt tgtcgccgct gcggatggcg gcggcttcgg
     5881 tgtcttgttt ccattctgtt ccgccggccg gcggggcggc ttcggatgcg gcggcttcag
     5941 gcggcatatc tgccgaaccg ccgtcttgag ccgtcggcgg cgtttcgccg gacaggaagg
     6001 ttttgatgcc gtctgaaagg gcgtaggcgt tttcttgcaa cgccgctccg ctccgccacc
     6061 atccgtaggc ggcaagcggt tcgagcgggc tgttgcggtg gtaggtctgc tgccagtagg
     6121 cgttgatcgg attttggctg aaccatacgg caatcagggc agacatcaat atggaagtga
     6181 aaagggaaag aaagtttttc atgtccaaac ccttaaaaat tggcataaat aaagccgggt
     6241 atccccgaag gggcgaggac gataatcagc agcaggacgg cggaaacggg gataaaccac
     6301 agccacatcg ggattttttc caaacctttg accgcgccgt cgaaagcgcg ttgcaggtaa
     6361 gggtagagca gcatgacgga tgcaaacgag gcaagcagca gcatatccgc ccgttgcggc
     6421 gcgttccagc cgccggcatt ggcaaagagg gcactgaaaa ctgcgcctgc atcgtcgggg
     6481 tttgcggtat tgaagacgac gaaggtcagg cagacaaaat ggaaggtgat gagccacgag
     6541 agcggcgcaa ggtatttcag acggcatagc gcgtcgcgtc cgaaataccg gtcgcccgtg
     6601 ttgagcagcg ccagtgccgt gccgtgcagc gcgccccaaa tgaggaagtt ccagccgtag
     6661 ccgtgccaga tgcctgagag taccattgcc gccatcaggt tgagctgtgt ccgcaaaaag
     6721 ccttttttgc tgccgcccaa ggggatgtag atgtagtcgc gtatccaggt ggaaaggctg
     6781 atgtgccatt tgtcccaaaa tgcgcggata ttcgcggcac gaagcggtgc ggagaagttt
     6841 ttgggcagcc ggaagcccag cagcatcgcc ataccgataa ccaaatcgga atatccggaa
     6901 aagtctaaaa agagttggaa ggtatagccg tacacgcctg ccaacacgcc ccagccgtcg
     6961 aattgggcgg gattttcaaa tacgggcgac acccagtttt ccgccagtat ccccgccagc
     7021 caccattttt tggcaatgcc cagtaaaatc agggaaacgg cgagggcggg gcggacgggc
     7081 gaacgcggtc ggcgggtgcg gatttgcgcc agtgcacccg cctgttcgcc gtccgtgctt
     7141 ttgaatgcgg cggcgcggat aatcgggccg gaggtaacgg tggggaaaaa actcaggtgc
     7201 agcagcagct cgtgccaccc aaaacgcgcg gcgtgcgggg cgcggaagca gtaaaccaga
     7261 taggcgacag actggaaggt ataataggaa agccccagcg gcatcaggat gtcgattgcg
     7321 ccgccttttc cggcatattg tgcaatcagc ggacggaaaa aatcgaaata tttgaaaaag
     7381 cccaaaacgg taatcgaggc ggcaatgccg caccccagcc aaaagcggcg cgtactttcg
     7441 cgatcggaac ggagcagttc gcccaaaagg tacacgcagg aggaataaag gacgatgatt
     7501 gccgcaaata cggggctaat atggtagagc cagcccatac cggcagccaa aagcagcagg
     7561 ttttggacgg acgggtattt cgcaaagccc cagtaaatcg gcaggaagac gataaaaaac
     7621 agtgcgaact cgacagacag cagcggcata aaccatcctt gggacaaaaa aataggcgtt
     7681 aaaacacggc aaagcccgtt attttaagca ggggcggata ttccttcaat caaaatgccg
     7741 tctgaagggc gggaggggct tcagacggca tcgctcggtc aggttcggat atggtggttc
     7801 aaattcaggc agacccgcaa acctgccgcc gccgtcagca tacccgcaac gccgatgcag
     7861 gaaatgcccg agtattgcgt aacctaatgt cccagcagtg cgccgccgcc tataccgacg
     7921 ttgtaaagct ccgaataaat cgagttggca aggtctgcgg catcggaggc gaaatccaaa
     7981 actttggcaa ccatccccaa gctgacgacc acaatcgccg tcccccatac gaacaccaac
     8041 gcgtaaatgg cggcaggaaa atgcgccaaa ggcagcaaaa gccccgaaga gagggcgata
     8101 agcgacaccg cgcccgccaa aaatgcgcgc ggatgtttgg caaaccattt gccgaacagg
     8161 taagacgcgg caaaacccgc caggccgtac agccccggaa ccaccgtaac ctgccgcgcg
     8221 gagaaaccgc cgatttggat gacgaagggc tcgatgtagc tgtatgcggt aaagtgcgcc
     8281 gtaatcaccg gcaccgttac cgcatacagc agcatcagtt ttttccgttt caaaagcagg
     8341 ggcaggctgc ttaaagaacc ggtattcacg ctcggcaggc gcggcaggct tttggcaagc
     8401 actgccatta ccgccgccga acacaggccg atcagcagaa aactcgcctg ccagcccaaa
     8461 tattgtccga ccatacgccc gagcggaatc cccgccacca tcgccatcac cgtccccgtg
     8521 ctgagcagcc ccaatgcctg attccctttt ccgacaaggg cgatgcgtac cgccagcgaa
     8581 gccgtaatgg accagaacac cgcgtgcgtc agcacaatcc ccacgcggct tgcgagtagg
     8641 atttcaaaac gccacgaggc aaacgacagg atgtggctga cagtaataca gcgcaaacag
     8701 gaccagcagc aggccgcgcc gttccataat tgcgcgtcag cagcatcagc ggcagcggca
     8761 gcgaagtcag cgcgacaatc cacgcataaa ccgtaatcat cacacccgtt tcggtcgccg
     8821 ccatgccgaa gcttcgtccg atgtcgctca aaagcgcgat ggggatgtat tcggccgtgt
     8881 tgaaaataaa tgcgccgatt gccaaggcaa cgatgctcaa ccaaggtttg tgcgaagcgt
     8941 tcatctcaat tccttatatg tcgcaccccg ccaaaagggg gcgcattatg gtgaattaac
     9001 aaaaaccggt acggcgttgc cccgccttag ctcaaaggga acgattccct aaggtgctga
     9061 agcaccaagc gaaccggttc cgtaccattt gtactgcctg cggcccgccg ccttgtcctg
     9121 atttttgtta attcactata cttttcttgt ctgccggacg gcaaaatcca aatcaggggg
     9181 aagcagccgc ctcccgttcg gagaaacact cgggacgctg ctttgaaaat cgttccatat
     9241 gttgaaacat tgccgcctcc cgattgggca aggttgccat tatgtcgcaa gcatccgtgc
     9301 cgcaccgttt ccgcgtttgc ccaagttcac gctccgactt acaataacgc ccgtcagatt
     9361 gcagtaccaa cgaaaggaaa aatcgtggaa accctcctcc tcgacatcgg cggcatgagc
     9421 tgcggcggct gcgtcaaaag cgttacccgg atattagaaa gcgtaaaagg cgtggcaagc
     9481 gtcgaagtca gccttgaaaa caaaagtgcg accgtcggat acgaccccgc gcaaaccgca
     9541 gcggaagcac taatcgaagc cgtagaagac ggcggctatg atgcggcgtt gaaataaagc
     9601 ggcaaaaatg ccgtctgaag cctttgcgtt tccccgatgc cgtctgaagc acgctccgcg
     9661 ccttcgggcg gcatttgatt tagttgaacg ggcaggatga attggttaaa cttgcgggca
     9721 atagtgcctt cagacaaaca aaatatgccg aacaaaaaga aaaaagccgc ctttgcgcgc
     9781 tgcaacggag aacggtgtgc cgggcgtgtc cgacatcaaa aattcatatg atgatttgat
     9841 gtacgaatcc aaagcatttt cccaaaccgc catcaacgcc ttggaggcat gcgcccgcat
     9901 gatgggtttg aagcctacgg cagccgcaaa agccaaggtc ttggagctgg gctgttcgat
     9961 gggcagaaac atcatcaacg caggcacttt attacccgga tgcggaattt gtcggtatcg
    10021 acttgtccgg caggcaggtt gcgcagggca acgccatcat tgaaaaaatg ggcttggaaa
    10081 atgtgcgtct ggaagaaaaa gatattttga ccgtcgatga atcattcggg aagttcgact
    10141 atatcatcgt ccacggcatt tggtcgcggg tgcccgacgc agttaaagac aaaatctttt
    10201 cgatttgccg gaacaacctg accgaatacg gcattgccta tatttcatac aatgtttacc
    10261 ccggttggaa gcggcaggag cagttgcgcg atattatgca gtttgccggc agggatgcgc
    10321 ttggagaacc cttggaagca aggacgcgga aaggcttgga tgcgatcaag gcgctggcgg
    10381 aaattttgga aaacgacaag ggcttgggcg gcggcaaact tccggcgatt caaaaaatat
    10441 tgaatcacaa tacttattat gtcgcacacg aatatatgga aatattcaac gacccgattt
    10501 acgttaacgg tttcattgaa tgggcaaacc gccaccggct ggcgtatatc ggggataccg
    10561 atttgcatgt gtcttttgtt tcttggatgg cggagcatac gcgggagcgg attctggcat
    10621 tggcaggggg tgattatatt gccaaagaat tttacagcga tattttatcc gaccgccaat
    10681 tccaccgttc gcttttatgc cgtgaggaag tcggggatac tgtcaggcgt gatgagtcgg
    10741 ttgccgtcga agtgatagaa agtttgaatt tccgcccggc aagaggggaa acaatcaact
    10801 ttgatgaaaa cgataccctg ctttcgggca tacgcgatgt gatgaaaacc ggagaggcgt
    10861 ttaaaacgga agatgttgcg gaaaatcttg cccgccgatt tcccggtctg gaatttgacc
    10921 gcatgaaaat caattcccag cttttattgc aaaccattct cgggcgtttt tctgtttcat
    10981 cagacaatgc gggcaaacca ttttttgaag accataaaac ctatgtgccg gcgcgcttta
    11041 caaactatgc cgccgccttt gtggaacacg gtgcgggagc gtttgtccgg cctgccaacc
    11101 gttacaacga aagcacgccc tcattcggat acgggcattt gtacattatg cggcaattgt
    11161 cgcggccgac gagcaaacag gcattgattg aaacggttgc cgaaaacttg aacatcgtca
    11221 gcgccacgcc cgacggtttg acattccatc cgcctgccga agtgtatgtg gaagaaatat
    11281 tggcagactt ggcagacagg cattttctcg tttcggcgga ttgacgggca agtcccgacg
    11341 gcaatccgtc cgatatattt tcagacagga aagacacaca ctgcgaaaaa cggtacacga
    11401 cacatatgaa ccagacattt accttgcccg atacgcgccc gtatccccaa aatccgatta
    11461 aaaaccacct gctgctcaat gcctaccagt tggcgcataa ttcttcccag gcttcgcgca
    11521 aactctcgtc cgggcagctt caaaccgaaa tcagggggat gcttgagcaa aaccactata
    11581 tcaacctttc cctcgcgctg acgatgtcgc ccgatgccgg aacttatgcc gcgctgcttt
    11641 ccagtgtgaa cgcggtactc gattgcgaga aagaaggcga agtgcagtgg ttcgccctgc
    11701 cggtcgtgct ggtgtcaggc tgcaagaaag aacgggcaat cgagatgaag ctgccgacgg
    11761 aggcattgtt tgcctgcctg caaaactatc cgcacctgcg cgcgttgacg caagagacac
    11821 aatggctgcc ttatcttgtg cattcttccg atttgagtgc ggtcgcgccg gatgagtggt
    11881 ggcgtgccaa acaaaatacc gaagcggcgg cgcaacactt acgccgtttc gccccgcgcc
    11941 ctttgctgtt gcccgaaggg cagtccgtcc acgtcgttta cgtgctgggt ttcggcagcg
    12001 gcaaagttca ggcggcattg gggcagaacc ttcttcaggc aggcttgccc ttaatgcagg
    12061 tatggcagga aaatcttgca tcggaaggca ttacgctgtt tgccaatccg ctttcccccg
    12121 ataccccggc gcgcgcgctt tcagacggca gccacacgcg ccaacgtatg gcgatggatg
    12181 tttttgcggc aaacgcgata cgcgccgtcc ggatgcaggg tccgcgcgtc ggcgtggtcg
    12241 ctgcggcaaa ggcgggcgga cagattctat tcggatttaa tgcgaccgac ggcgcgtttg
    12301 aagtcgtgcc gcaggtgttt tgctggcagc tttcctttac cgataacatt gccgtcatcc
    12361 agcagaattt cctcgacctg atggcagaat gccgtgtaga acacgtttac ctgctgcaca
    12421 atcccttggg cgaacaggaa aacatcccga gctatgcgga agcgttgaaa cgggaagggc
    12481 gcaatccgtt tttcagcgca tagtgatttg tagtagtgat aggctttctc atttctataa
    12541 taaatcaaaa taaagagcac aacacttttt cattctgtgt tgtgccttga gtgaaacgaa
    12601 aggatgaatt ataaatatga agattgtaat tgcaccggat tcgtttaagg aaagcttgac
    12661 agctcaacag gtagctgaag caataaaaag aggcttccaa caatcgatag cagatgtgga
    12721 atgtctcctc tgtcctgtcg gtgatggggg agaaggcact gtagatgcta tccgacattc
    12781 tcttgaccta gaagaaaaat gtctccaagt gacaggacct tttggacaaa aaaagtcatg
    12841 cgctattttc aaaaagaaca actagcccta ttcgaagttg ctgatttggt tggtcttgga
    12901 aaaatcccgc tagagaaacg aaatccatta caaatccaaa ctcgtggtat tggagaattg
    12961 attcgccacc tcattagtca agaaattaaa gaaatctata ttggcgttgg cggtacggcc
    13021 ggtaatgatg gaggtattgg gattgctgct ggtttaggtt atcaatttta tgatgaggat
    13081 ggaaatgcct tacccgcttg cggtcaatcc ttactaaacc cagcttctgt ttcaacagaa
    13141 aatcgctatg aaattcctga agatgttcac attcgtattt tagcagatgt tgtgagtccc
    13201 ttatgtggac accaaggtgc gacctatacg tttggcaagc aaaaagggct ggattctact
    13261 atgtttgagg ccgtagatca ggcaatacaa gatttttatg aaaaagtccc ccctgcaaca
    13321 ttaaaactta aaggagcagg agctggtgga ggcatcgctg gtggtttgtg tgcctttgct
    13381 caggcaagta tcgtatctgg aattgacacc tgcctggact tgattgactt tgataagaaa
    13441 gtgtcagatg ttgacttggt tatcgttggt gaaggaagac tagatcgtca aagtttagca
    13501 gggaaagcgc ctattggtgt agcaaaaaga acccctgtcg gagttcctgt tgtcgctatt
    13561 tgcggcagtt tcgctgaaga tttgccttcc ctaccatttg aaaatatcca agccgccttt
    13621 tctattttgg agaaaagtga acctttagaa gatagtttga aaaatgccag tctttatttg
    13681 gagcatacgg cttctaatat tggacattta ttaaatatgc ctaagattta gccaagccat
    13741 ttcttccaga tggatgtttt ggcaggttct tccttatgcg gttacgatta accggccgat
    13801 gccgtctgaa agggtttccg cttcagacgg catcgcgttg gaacagggat gggatatgaa
    13861 aaaaccgaaa atcctttttg tctgcctcgg caacatctgc cgttcgccga tggcggaata
    13921 cattttgcgc cgccgtgccg ccgaagcggg cattcccctc gaaacggaca gcgcagggac
    13981 atcgggctgg cacgacggcg aggatatgca ccgtgaaacg gcaaagatat tgaaaaaata
    14041 cggcattgac gcttcaggct ttaccagccg caaaatccgc caaagcgatg cgacggcgtt
    14101 tgactacatc atcgctatgg acggcaagaa tttgtccgaa ttggaaaaaa ctttcggcag
    14161 gcggccggaa aaaatattca agctgaccga cctgataccc gaaagcggtt acgaccatgt
    14221 ccccgatccg tggtacatgg gtgattttga agaaacatac aggcttgcgg atgcaggctg
    14281 tcgggcatta ttggaaaaga tttccaaata aagcaattga atacaaacac aaatataaaa
    14341 ccgcctcctg tcgtatcggt cgttcggacg gcataaacag ggaatttatg aaaacaaatt
    14401 tcaaacagaa aattatcgaa caggcacgca gagaggactt gcaggtaacc gctttgcgcg
    14461 agcaggtatt agatattgtc ttgcagcaaa gcggcgtgat taaagcctac aatgttttgt
    14521 cacagatgca gcagcagagc gaaggcgtgc ttgcaccgcc taccgcctac cgtgcccttg
    14581 atttttgggc ggatcagggc gttttacaca aagtggcggc ggtcaacggc tatattttgt
    14641 gcagccacgc gcagcacgag tgcaacgacc attgccacga ccacgaagaa gccgaagcgc
    14701 accacagcgc gtttattttg gtctgcaccg aatgcggcgc ggcggacgag caaaccctca
    14761 gccacgagtg ggcggcactg cgtgcaggcg ttgccgaaag cggttttgcg ctgaaagaag
    14821 aacacgttgt tttaactgga atctgtaaaa aatgtcagaa gtgaaaaaaa ccaaagtcca
    14881 cctgatttca ggttttctgg gaacaggcaa aaccaccgcg ctcaaaagcc tgatggcgca
    14941 aaaagacccg aacgaaaaat gggtcatcat cgtcaacgag ttcggcgaaa tcggcattga
    15001 cggcgcggta ttgagcgaca acggcatccc tgtggcagaa atcgccggcg gctgtttatg
    15061 ctgcaccgcc ggcccgcaaa tgggcgtaac cgtgcagaaa atgctgcgcg acgccaaacc
    15121 cgaccgcctg atgattgagg caagcggact ggcgcacgcc gccagcgtca tcgacgaatt
    15181 gaaagccaaa cctctggaca gtcttttgga aatcggcgcc gtctttaccg tcgtcgatcc
    15241 gcgccagttc atcaatcccg attacgcgca acaggcgttg tataaagacc aaatcggcat
    15301 ttgcgacgta ttggccgccg gcaaaaccga tttatgcacc cccgaacagc ttgccgaatt
    15361 tcatgacaaa gcggcaaaac tgttcccacc caaagccaaa gtggtcgaag tccaaaacgc
    15421 acaactcgat atccaatggc tcgatattcc cgtagttgaa aaatcacgct accgcctcaa
    15481 agccctgccg gacaacacca tgggcttcca gtcgcaaggt ttcacattcc ccgccggacg
    15541 cgatttcgac ggcgaaaaat tgaccaactt cttcaatgat ttgcccaaaa tgaccgaagg
    15601 actcgtccgc gccaaaggcg tgttccaagt attgggaacg tgggtgtggc tcaactgggt
    15661 ggacgggcag tggggcgcga accaagtgtc ttggcggcgc gattcgcgtt tcgagctgat
    15721 tgccaaatcg tttgacgcgg atttaatcga acaaaaactt aaagacgcat tggaatagcg
    15781 ttcacgcgct ttcagacggc atatgaaatg aaaatgccgt ctgaaggctt taggaggacg
    15841 ggatgaaacc caaattcaaa accgttttaa ccgcgctgct tttggcggtt tccctgccgt
    15901 ctatggcggc aacccgcgtc ctgatggaaa ccgatatggg caatatccgt ttggttttgg
    15961 acgaatccaa agcctccaaa accgttgcca atttcgtgcg ctatgcccga aaaggctttt
    16021 acgacaacac gattttccac cgcgtcatcg gcggcttcgt catccaaggc ggcggattga
    16081 ccgaggactt ggtgcaaaag gcaaccgata aggccgttgc caacgaatcc ggcaacggct
    16141 tgaaaaacac cgtcggcacc atcgcaatgg cgcggacggc agaccccgat tccgccgccg
    16201 cccaattctt tatcaatctg gcggacaacg gttcgctcga ctacaaaaac ggacaatacg
    16261 gctacaccgt tttcggcagg gtagaaagcg gaatggacac cgtttccaaa atcgcccgcg
    16321 tcaaaaccgc cacgcgcggc ctttatcaaa acgtacccgt acagcccgtc aaaatccgtc
    16381 gcgttgttgt cgggcagtaa cacgcagaca gacgttcaga cggcgtcgcc cgtttcccaa
    16441 aaaacgccgt ttaaagtaaa aaaatatttt aaaacagaca gttgatattg acaaattcaa
    16501 accgaggatt ttaaaatgct
//
LOCUS       DAXDXW010000057        16365 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXDXW010000057 DAXDXW010000000
VERSION     DAXDXW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16365
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.058"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            306..1634
                     /gene="miaB"
                     /locus_tag="ACFB64_001515"
     CDS             306..1634
                     /gene="miaB"
                     /locus_tag="ACFB64_001515"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC1209850.1"
                     /translation="MKKVFIRTFGCQMNEYDSEKMLSVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKIVEA"
     gene            1652..1816
                     /locus_tag="ACFB64_001516"
     CDS             1652..1816
                     /locus_tag="ACFB64_001516"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209851.1"
                     /translation="MPPEAFQTAFCLVSADGTAAVIRTSGINFQTRNGTPSTARKPPP
                     EPFLPPAGFQ"
     gene            complement(1881..3794)
                     /gene="dxs"
                     /locus_tag="ACFB64_001517"
     CDS             complement(1881..3794)
                     /gene="dxs"
                     /locus_tag="ACFB64_001517"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC1209852.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAATDKLLGGDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKEGGAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTEHGDPKKLLDDLGLSAEAVER
                     RVREWLPDRDAAN"
     gene            complement(3879..4796)
                     /gene="xerC"
                     /locus_tag="ACFB64_001518"
     CDS             complement(3879..4796)
                     /gene="xerC"
                     /locus_tag="ACFB64_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC1209853.1"
                     /translation="MVLDGFAAYFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLDGRTLARKLSAWRQYCAWLVKRGLMRADP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLESWAAQYGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4990..6054
                     /gene="fba"
                     /locus_tag="ACFB64_001519"
     CDS             4990..6054
                     /gene="fba"
                     /locus_tag="ACFB64_001519"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC1209854.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLLEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAAGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLGKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            6193..7050
                     /locus_tag="ACFB64_001520"
     CDS             6193..7050
                     /locus_tag="ACFB64_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HFC1209855.1"
                     /translation="MTRSKPVNRTTFCCLSLTAGPDSDRLQQRRGGGGGVAADIGTGL
                     ADALTAPLDHKDKGLKSLTLEASIPQNGTLTLSAQGAEKTFKAGGKDNSLNTGKLKND
                     KISRFDFVQKIEVDGQTITLASGEFQIYKQDHSAVVALRIEKINNPDKIDSLINQRSF
                     LVSDLGGEHTAFNQLPDGKAEYHGKAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPE
                     QNVELASAELKADEKSHAVILGDTRYGGEEKGTYRLALFGGRAQEIAGSATVKIGEKV
                     HEIGIADKQ"
     gene            7189..7866
                     /gene="tsaB"
                     /locus_tag="ACFB64_001521"
     CDS             7189..7866
                     /gene="tsaB"
                     /locus_tag="ACFB64_001521"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC1209856.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGEIRLFHQEVGIRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIALPEGYVFSDG
                     IGSAFALENRPPFSGKPDMPTAADFLALALGGGYPATGAAHAELLYVRNKIALTAKEQ
                     AERKART"
     gene            7863..8303
                     /gene="rimI"
                     /locus_tag="ACFB64_001522"
     CDS             7863..8303
                     /gene="rimI"
                     /locus_tag="ACFB64_001522"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC1209857.1"
                     /translation="MNIRPALPSDCAALAALDTVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGRIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWYAHLPEGTQRLLLE
                     VRAGNAAAQALYTKHGFSIAGRRKNYYCAADGQTEDAVLMEKIC"
     gene            8297..9034
                     /locus_tag="ACFB64_001523"
     CDS             8297..9034
                     /locus_tag="ACFB64_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC1209858.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAATIRPSQPHNVQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGILLDNILKAAGLDAAYVHKTCWVKTAAVGNPMP
                     SEQAVADALGQIAGELDGCRAPAVLFLGQAFVNLERQAMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKRALRQGGGS"
     gene            9096..9737
                     /gene="pyrE"
                     /locus_tag="ACFB64_001524"
     CDS             9096..9737
                     /gene="pyrE"
                     /locus_tag="ACFB64_001524"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC1209859.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGA
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DRGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRTYRRQYGVE"
     gene            9811..10239
                     /locus_tag="ACFB64_001525"
     CDS             9811..10239
                     /locus_tag="ACFB64_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC1209860.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTHAISKKQISRDEIADILNGGTTLHDTPPATAAAA
                     PAAAPQVSVPPARQEGLNWTIATLFALIVLIMQLSYLFIL"
     gene            10236..11546
                     /gene="argA"
                     /locus_tag="ACFB64_001526"
     CDS             10236..11546
                     /gene="argA"
                     /locus_tag="ACFB64_001526"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC1209861.1"
                     /translation="MNAPDSFVAHFREAAPYIRQMRGTTLVAGIDGRLLEGGTLNKLA
                     ADIGLLSQLGIRLVLIHGAYHFLDRLAAAQGRTPHYCRGLRVTDETSLGQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWMPPLGHSYGGKTFNLDMVQAAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAASETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNPHILVRRLHR"
     gene            11642..13657
                     /locus_tag="ACFB64_001527"
     CDS             11642..13657
                     /locus_tag="ACFB64_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC1209862.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQNAEYPKGVYRMCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLNKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPAYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGGAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADSKWQEAELPHLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRLQPQQFVSDGIEVRQFWAVSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSSPKHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEACKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQRESADKLACVLLFLKEFLG"
     gene            complement(13770..14726)
                     /locus_tag="ACFB64_001528"
     CDS             complement(13770..14726)
                     /locus_tag="ACFB64_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC1209863.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLKM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDSGGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAGTLTAAACPLLQRPFESDGFGLLADEADALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILNSEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGRLRRLRLEHAL
                     ALIESGSTIQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            14962..15933
                     /locus_tag="ACFB64_001529"
     CDS             14962..15933
                     /locus_tag="ACFB64_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC1209864.1"
                     /translation="MKPRFYWAACAVLPAACSPEPAAEKTVSAASQAASTPVATLTVP
                     TARGDAVVPKNPERVAVYDWAALDTLTEPGVNVGATTAPVRVDYLQPAFDKAATVGTL
                     FEPDCESLHRHNPQFVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLSR
                     IFGKEARVAELNAQIDALFAQKREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGD
                     IGLPPVDESLRNEGHGQPVSFEYIKEKNPGWIFIIDRTAAIGQEGPAAVEVLDNALVC
                     GTNAWKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAQ"
     gene            15966..16289
                     /locus_tag="ACFB64_001530"
     CDS             15966..16289
                     /locus_tag="ACFB64_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC1209865.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGNMTIREGEMAVVPKSVSHRPRSENGCSVVLIDLPD
                     PSEAA"
BASE COUNT         3669 a         5041 c         4458 g         3197 t
ORIGIN      
        1 ggtttaaatt taatccacta taacgcagga actgatgttc cctgtcgccg aaattgctgg
       61 tacacgcaca cagcagcaat gccgcccata caaccggttt catacacacc tcccattaaa
      121 gccaaacatt atacagccgt tccgaccgat taaattcata ttttaaaaca atattttgcc
      181 tcccaaaccc acatcgtgct ataatccgca ccgattttca gacggcatcg tcgtgccgtc
      241 tgaaattttt tcattccaac aaccatcagc cccgcgatta cggctgcccg agaaagacac
      301 aaaccatgaa aaaagtattt atccgcacct tcggctgcca gatgaacgag tacgacagcg
      361 aaaaaatgct gtccgtcctt gccgaagaac acggcggcat cgaacaggtt acccaagccg
      421 acgaagccga catcatcttg ttcaacacct gttccgtgcg cgaaaaagca caggaaaaag
      481 tgttctccga cttggggcgc gtccgtccgc tcaaagaaaa aaaccccggc ctcatcatcg
      541 gcgttgccgg ctgcgtcgcc tcgcaagaag gcgaaaacat catcaaacgc gcgccttatg
      601 ttgacgtggt tttcggcccg caaacgctgc accgcctgcc caaaatgatt gtggacaaag
      661 aaaccagcgg gctgtcgcaa gtcgatattt ccttccccga aatcgaaaaa ttcgaccacc
      721 tgccgcccgc ccgcgtcgaa ggcggcgcgg catttgtgtc gattatggaa ggctgttcca
      781 aatactgctc cttctgcgtc gtcccctaca cgcgcggcga agaattctcc cgcccgctca
      841 acgacgtatt gaccgaaatc gccaaccttg cccagcaagg cgtgaaagaa atcaacctct
      901 tgggacaaaa cgtcaatgcc tatcgcggcg aaatggacga cggcgaaatc tgcgacttcg
      961 ccaccctgct gcgtatcgtc cacgaaatcc ccggcatcga acgtatgcgc ttcaccacca
     1021 gccacccgcg cgagtttacc gactcgatta tcgagtgcta ccgcgacctg cccaaactgg
     1081 tttcccacct gcacctgccg attcaaagcg gttccgaccg cgtattgagc gcgatgaaac
     1141 gcggctacac cgctttggaa tacaaatcca tcatccgcaa actgcgcgcc atccgtcctg
     1201 atttgtgcct gagcagcgac ttcatcgtcg gtttccccgg cgagaccgaa cgcgagttcg
     1261 agcaaacctt gaaactggtg aaagacattg ccttcgactt gagcttcgtg tttatttaca
     1321 gcccgcgccc cggcacgcct gccgccaacc tgccggacga cacgccgcac gaagaaaaag
     1381 tgcgccgcct cgaagccttg aacgaagtca tcgaagccga aaccgcccgc atcaaccaaa
     1441 ccatgatcgg cacggttcaa cgctgcctgg tcgaaggcat ctccaaaaaa gaccccgacc
     1501 agctccaagc ccgtaccgcc aacaaccgcg tcgtcaactt taccggcacg cccgacatga
     1561 tcaaccaaat gatcgatttg gaaatcaccg aagcctacac cttttccctg cgcggcaaaa
     1621 ttgtcgaagc ctaaaaccct cacgccgaaa aatgccgcct gaagcgtttc agacggcatt
     1681 ttgccttgta tcggcagacg gcaccgccgc cgttatacgg acaagcggaa taaatttcca
     1741 aacgcgcaac ggcacaccaa gcaccgcccg aaagccgccg cccgaaccct tcctgccgcc
     1801 tgcggggttt caataaaaaa accgcagaaa caaacgccgt ttaaaaacgc cgcccgataa
     1861 cgacggcgcg gtcaagcggt ttaatttgcc gcatcacggt ccggcagcca ctcgcgcacc
     1921 cggcgttcca ccgcttcggc actcaaaccc aaatcgtcca aaagtttttt cggatcgccg
     1981 tgttcggtta cggtatcggc aacgcccaaa agcaaaacgg gtttgcagat gccgtgtttc
     2041 gccaacactt ccaagaccgc gccgcctgcg ccgccctgtt cggcgttttc ttcaagggta
     2101 acgatgcggt cgtggcttcg ggcaaggcgg acaatcaact cttcgtctat cggtttgacg
     2161 aagcgcatat cggcgacggt ggcgttcagt ttttcggcaa ccgccaatgc gggggcgacc
     2221 atactgccga aggcaatgaa ggcggttttc tcaccttcgc ggcggataat gcccttgccg
     2281 atttccacgg tttccatgcc gtctgaaacc ggcgcgcccg tacccgtgcc gcgcggatag
     2341 cggacggcgg cgggcgcatc cgcctgatag caggtcgaaa gcagcaggcg gcattcgttt
     2401 tcatcgctcg gcgcggcaac aatcatgttc ggcacacagc gcaagaagct caaatcgtac
     2461 aagccggcat gggtcgggcc gtccgcgccg acgatgcccg cacggtcgac ggcaaacaaa
     2521 acgggcaggt tttgcagggc gatgtcgtgc accagttggt cgtaggcgcg ttgtaaaaag
     2581 gtggaataaa tcgccacgac gggcttcatg ccttcgcacg ccaaaccgcc ggcaaaggta
     2641 acggcgtgct gctcggcgat gccgacatcg aaatagcggt cggggaatcg ttgttcaaac
     2701 tccaccagtc cgctgccctc gcgcatggcg ggggtaatcg caaccagtcg ggaatctgcc
     2761 gccgcccggt cgcacagcca tttgccgaat acttgggtat aggtcggttt ggcggcgggc
     2821 ttgggttctt tttcagacgg catttgcgcc ccgccttctt taggcaggtt ggcgacggcg
     2881 tggtatttga cggggtcgtt ttcggcgagt ttgtagccgt tgcccttttt ggtgatgacg
     2941 tgcagcaact gagggccttt gcggctgcgc aagtctttca atacgtccac cagattctcg
     3001 acgttgtgtc cgtccacggg gccggtgtag cggaagccga aattttcaaa cagcgacagc
     3061 gactgtttgg cgtgttcggc ttcttcggca agggttttga ttttgtgttc gactttttgg
     3121 gcaaactcca tcgcgccggg tattttgtct aataccttgc ccgtttgcgc tttgacggta
     3181 ctcaacagtc cgtgcatatc gcgcacgacg ttgctggcaa gatatttggg caacgcgccg
     3241 acgttggggg aaatcgacat ttcgttgtcg ttgaggacga ccagcaaatc cacatccata
     3301 tcgcccgcgc aattcaaggc ttcaaacgcc tgccccgccg tcatcgcgcc gtctccgatg
     3361 atggcgacgc tgcggcggtc gccgcccaag agtttgtccg tcgccgccat gcccaaagcc
     3421 gcgccgatgg aggtggagga atgccccacg ccgaacgcgt cgtactcgga ctcgcaacgt
     3481 ttcggaaaac ccgccaaacc gccgtattgg cgcatggtgt gcatctggtt tttcctgcct
     3541 gtcaggattt tgtgcgggta gctttggtgt ccgacatccc acaccagctt gtcttcgggc
     3601 gtgtcgtaca catagtgcag ggcgatggtc agttcgaccg cacccagatt gctggcgaaa
     3661 tgcccgccgg tctgcccgac agattccagc agaaaggcgc gcaactcgcc ggcaaggcgc
     3721 ggcagctgtt ttttgtccag gcggcgcaaa tcttgcgggc tgtcaatcag gtcgagtagg
     3781 gggcttgggt tcatggtgtg tcttttttat gtgtcgtccg ggtgcaacgg tcaattatat
     3841 atcaagagcg tgcggctgac ggctgatttt gccgtacgtc attcgtcctg ccgcttggcg
     3901 cgcgggtggg cttcgtcata gaggcgggcg atgtggtcga aatcgagctt ggtataaatc
     3961 tgcgtggtcg aaaggctgct gtgtccgagt agctcctgca ccgccctgat gtcgcgcgaa
     4021 gcctgcaaca ggtggccggc gtagctgtgg cgcatcatat gcggcgaaac gtgcctgccg
     4081 tcgccgtatt gcgccgccca cgattcgagg cgtttttgga tttggcgttg gctcaggcgc
     4141 gtgccgttcc tgccggtaaa cagggctttg ccgtccgatg ccgtctgacg cagcggcaga
     4201 tagtttttca aggcttccac gcttttgccg gtcagcggaa cctgacgctg cttgcgccct
     4261 ttgccggtaa cgtgtaccca cgcttcgtcc aaatatacat catctgcatt caagccgtgt
     4321 atctcgctca cgcgcaaacc gctgccgtac atcagctcga acagcgcgtg gtcgcgcacc
     4381 gccagcgggt cgccgccgtc cacgggcaaa tccaacatcc ggttcagcca ttcctgcggc
     4441 agggctttgg gtacgcgctc gggctgcttc ggcggtttga tgtcggcggt cgggtcggcg
     4501 cgcatcagcc cgcgtttgac cagccaggcg caatactgcc gccacgccga cagcttgcgc
     4561 gccagcgtcc gaccgtccaa accgcgctgc gacagccgcc gcaacgccgc cgtaaaatcg
     4621 cgccgcgaca agtcctgcgg cacgccgcct gcatcttcag acggcatttg tgccaacagt
     4681 gcaaacagtt cttccaaatc gcgccggtat gccgcaaccg tgtgctccga cttgccctcg
     4741 cgcacgatgt tttccaaata agcgtcaaaa tacgccgcaa acccgtccaa aaccataacc
     4801 gtcccacaca aatatcaaaa aaccagtgaa aacattatca taaacaacac accaaaatcc
     4861 cgaaccgccc aaaatccgcc ccatctgccc gccgttatcg ttaatataaa atcaatgagc
     4921 cgtttatggt tttttgccgt aaaaaacatt ataatccgcc ttatttacct attgcccaag
     4981 gagacacaaa tggcactcgt atccatgcgc caactgcttg accacgccgc cgaaaacagc
     5041 tacggcctgc ccgcgttcaa cgtcaacaac ctcgaacaaa tgcgcgccat tatggaagcc
     5101 gccgaccaag tcaacgcgcc cgtcatcgta caggcgagcg caggtgcgcg caaatacgcg
     5161 ggcgcgccgt ttttgcgcca cctgattctg gcggcagtcg aagaatttcc gcacatcccc
     5221 gtcgtgatgc accaagacca cggcgcatcg cccgacgtgt gccaacgctc catccaactg
     5281 ggcttctcct ccgtgatgat ggacggctct ttgctcgaag acggcaaaac cccttcttct
     5341 tacgaataca acgtcaacgc cacccgtacc gtcgtcaact tctcccacgc ctgcggcgtg
     5401 tccgtcgaag gcgaaatcgg cgtattgggc aacctcgaaa ccggcgaagc aggcgaagaa
     5461 gacggagtgg gcgcggcagg caaactctca cacgaccaaa tgctcaccag cgttgaagat
     5521 gccgtgcgtt tcgttaaaga taccggcgtt gacgcattgg cgattgccgt cggcaccagc
     5581 cacggcgcat acaaattcac ccgtccgccc acaggcgacg tattgcgtat cgaccgcatc
     5641 aaggaaatcc accaagccct gcccaataca cacatcgtga tgcacggctc cagctccgtt
     5701 ccgcaagaat ggctgaaagt catcaacgaa tacggcggca atatcggcga aacctacggc
     5761 gtgccggttg aagaaatcgt cgaaggcatc aaacacggcg tgcgcaaagt caacatcgat
     5821 accgacctgc gcctcgcttc caccggcgcg gtacgccgct accttgccga aaacccgtcc
     5881 gactttgatc cgcgcaaata cttgggcaaa accattgaag cgatgaagca aatctgcctc
     5941 gaccgttatc ttgcgttcgg ttgcgaaggt caggcaggca aaatcaaacc tgtttcgttg
     6001 gaaaaaatgg caagccgtta tgccaagggc gaattgaacc aaatcgtcaa ataacaggtt
     6061 gcctgtaaac aaaatgccgt ctgaacgccg ttcggacggc atttgatttt tgcttctctg
     6121 acctgcctca ttgatgcagt atgcaaaaaa agataccata accaaaacgt ttatatatta
     6181 tctattctgt gtatgactag gagtaaacct gtgaaccgaa ctaccttctg ctgcctttct
     6241 ttgaccgccg gccctgattc tgaccgcctg cagcagcgga ggggcggagg cggtggtgtc
     6301 gccgccgaca tcggcacggg gcttgccgat gcattaaccg cgccgctcga ccataaagac
     6361 aaaggtttga aatccctaac attggaagcc tccattcccc aaaacggaac actgaccctg
     6421 tcggcacaag gtgcggaaaa aactttcaaa gccggcggca aagacaacag cctcaacacg
     6481 ggcaaactga agaacgacaa aatcagccgc ttcgacttcg tgcaaaaaat cgaagtggac
     6541 ggacaaacca tcacactggc aagcggcgaa tttcaaatat acaaacagga tcactccgcc
     6601 gtcgttgccc tacggattga aaaaatcaac aaccccgaca aaatcgacag cctgataaac
     6661 caacgctcct tccttgtcag cgatttgggc ggagaacata ccgccttcaa ccaactgcct
     6721 gacggcaaag ccgagtatca cggcaaagca ttcagctccg acgatgccgg cggaaaactg
     6781 acctatacca tagatttcgc cgccaaacag ggacacggca aaatcgaaca cctgaaaaca
     6841 cccgagcaga atgttgagct tgcctccgcc gaactcaaag cagatgaaaa atcacacgcc
     6901 gtcattttgg gcgacacgcg ctacggcggc gaagagaaag gcacttaccg cctcgccctt
     6961 ttcggcggcc gcgcccaaga aatcgctggc tcggcaaccg tgaagatagg ggaaaaggtt
     7021 cacgaaatcg gcatcgccga caaacagtag tatttgaaaa atgccgtccg aacacgataa
     7081 tttaccgttc ggacggcatt tttgcgccat accgccctag gacacgacgg cacggcaggc
     7141 aagccgattt cactatataa tgccgtctga accaacgaga accacaccat gcaagccgat
     7201 ttcaaccgcc ccgtcctcgc cgtcgatacc ggaacttcct atttgtcgct cgcgctgcgc
     7261 gccgacggcg aaatccgtct gttccatcag gaagtcggca tccgccagtc cgaactgatc
     7321 ctgccggaaa tccgcaccct attccgaaat gcgggcatta ccgccgccga tttgggggcg
     7381 attgtttatg cgaaaggccc cggcgcgttt accggactgc gtatcggcat cggcgtggcg
     7441 caaggtttgg caacgccgtt cgacacgcct ttaatcggca tccccacgct cgatgccgcc
     7501 gcctcgctgc cgccgccgca aagctgcata ctcgccgctg cggacgcgcg tatgggcgaa
     7561 gtgttttatg cgtggttcga cacgctgaac cgccgccgtt tgagcgatta tcaagtcggt
     7621 agggcggcag acattgccct ccccgagggg tacgtctttt cagacggcat cggcagtgcg
     7681 ttcgcactgg aaaaccgtcc gcccttctca ggcaaacccg atatgccgac cgccgccgat
     7741 tttctcgcgc tcgccctcgg cggcggctat cccgcaactg gcgcggcaca cgccgaactg
     7801 ctctacgtcc gcaacaaaat cgccctgact gccaaagaac aggcggaacg gaaggcacga
     7861 acatgaacat ccgcccagcc ttaccgtccg actgcgccgc actggccgcg ctcgataccg
     7921 tctgcaaccc gtccgcgtgg acgcagcgcc aatttgagtc cgcgcttgtt tcgccgtccg
     7981 aacaggtttt ccttgcggaa aaagacggca ggattgccgc ctttatcgtt tggcagaacc
     8041 tgcccgacga atccgaactg cacctgattg ccaccgcgcc cgaatgccgc cgacggggcg
     8101 ttgcgtccgc cctgctcgaa tattggtacg cacatctgcc cgaaggcacg caacgcctgc
     8161 tgctcgaagt ccgcgcaggc aacgccgccg cacaggcact gtataccaaa cacggcttca
     8221 gcattgcggg caggcggaaa aactattact gcgccgccga cgggcaaacc gaagatgccg
     8281 tcttaatgga gaaaatatgt taagcgcgcg ctacctccac ctgcacgagg ctttgggctt
     8341 gggtccgatg tggctgaaac aggccgccgc cgtcctgccg cccaaaaaca cacccgcaac
     8401 cccggcacag gcacgtcccc aaaccgtccg cgccgccacg atccgccctt cccaacccca
     8461 taacgttcag gcgcggctcg aaacgatgaa agcgttggaa accgccgccg tacatacgcg
     8521 caaacccgcg cctgaaaccg aaacgccccc gcccggcctt tcagacggca tcgcccccgt
     8581 tcccgccgct tcgggcatca ccaagcttgc cgtcgtcagc ctgtgtccgc cgatcgagga
     8641 tgcggtttac gggcaactgt tccacggcaa agcgggcatc ctgctcgaca acatcctcaa
     8701 agccgcagga ctggatgccg cctatgtcca caaaacctgt tgggtgaaaa ccgccgccgt
     8761 cggtaacccg atgccgtctg aacaggccgt cgcggatgcg ctgggtcaaa tcgccgggga
     8821 actcgacggc tgccgcgccc ccgccgtcct attcctcgga caggcgtttg tcaacctgga
     8881 acggcaggcg atgattgaaa ctttgtgcgg cagccgtccc ttcttcatca tcgaccatcc
     8941 cgcccggctg ttgcgccaac ccgaactcaa agcccgaaac tggcaggtgt tgaaacagtt
     9001 gaaacgcgcc ttgcggcaag gcggcggcag ttgaagcgcg ccgcacgggg cggtagaatc
     9061 gcaactgcgt cccaatatct gacagaaagc acaaaatgac tgatttccgc caagatttcc
     9121 tcaaattctc cctcgcccaa aatgttttga aattcggcga atttaccacc aaagccggac
     9181 ggcggtcgcc ctatttcttc aatgccggcc tcttcaacga cggcgcgtcc acgctgcaac
     9241 tggcaaaatt ctatgcacaa tccatcattg aaagcggcat ccgattcgat atgctgttcg
     9301 gccccgccta caaaggcatt attttggcgg cggcaaccgc gatgatgctg gcggaaaaag
     9361 gcgtgaacgt cccgtttgcc tacaaccgca aagaagccaa agaccgcggc gaaggcggcg
     9421 tgttggtcgg cgcgccgctt aaagggcgcg tgctgattat cgacgacgtg atttccgccg
     9481 gcacatccgt acgcgaatca atcaaactga ttgaagcgga gggtgcaacc cccgccggtg
     9541 tcgccatcgc gctcgaccgc atggaaaaag gcacgggtga attgagcgcg gttcaggaag
     9601 tggaaaaaca atacggcctg cccgtcgccc ccatcgccag cctgaacgat ttgtttatcc
     9661 tgttgcaaaa caaccccgaa ttcggacagt tcctcgaacc cgtccgcacc taccgccggc
     9721 agtacggcgt agaataaaaa taaagcatat gccgtccgaa ccacctgccg cttcagacgg
     9781 catcaaaccg acacacaccg agaaaacatc atgcccgcct gtttctgtcc gcactgcaaa
     9841 acccgcctct gggtcaaaga aacccagctc aacgtcgccc aaggcttcgt cgtctgccaa
     9901 aaatgcgaag ggctgtttaa agccaaagac catctggcaa gcacgaaaga acctatattc
     9961 aacgatttgc ccgaagctgt ttcggatgtc aaactcgtcc accgcatcgg cacgcacgcc
    10021 attagcaaga aacagatttc ccgcgacgaa atcgccgata tcctcaacgg cggtacaacc
    10081 ctgcacgata cgccgcccgc aaccgccgct gccgcacctg ccgccgcacc gcaggtttcc
    10141 gtaccgcccg cccgtcagga agggctcaac tggactattg caaccctgtt cgcacttatc
    10201 gtcctcatta tgcagctttc ctacctcttc atcctatgaa cgcgcccgac agctttgtcg
    10261 cccacttccg cgaagccgcc ccctacatcc gccaaatgcg cggcacgaca ctggtcgccg
    10321 gcatagacgg ccgcctgctc gaaggcggca ccttaaataa gctcgccgcc gacatcgggc
    10381 tgttgtcgca actgggcatc cgactcgtcc tcatccacgg cgcgtaccac ttcctcgacc
    10441 gcctcgccgc cgcgcaaggc cgcacgccgc attattgccg gggtttgcgc gttaccgacg
    10501 aaacctcgct cggacaggcg cagcagtttg ccggcaccgt ccgcagccgt tttgaagccg
    10561 cattgtgcgg cagtgtttca ggattcgcgc gcgcgccttc cgtcccgctc gtatcgggca
    10621 acttcctgac cgcccgtccg ataggcgtga ttgacggaac cgatatggaa tacgcgggcg
    10681 ttatccgcaa aaccgacacc gccgccctcc gtttccaact cgacgcgggc aatatcgtct
    10741 ggatgccgcc gctcgggcat tcctacggcg gcaaaacctt caatctcgat atggtgcagg
    10801 ccgccgcttc cgtcgccgtc tcgcttcagg ccgaaaaact cgtttacctg accctttcag
    10861 acggcatttc ccgccccgac ggcacgctcg ccgaaaccct ctcggcacag gaagcgcaat
    10921 cgctggcgga acacgccgcc agcgaaaccc gacgactgat ttcgtccgcc gttgccgcgc
    10981 tcgaaggcgg cgtgcatcgc gtccaaatcc tcaacggggc cgccgacggc agcctgctgc
    11041 aagaactctt cacccgcaac ggcatcggca cgtccattgc caaagaagcc ttcgtctcca
    11101 tccggcaggc gcacagcggc gacatcccgc acatcgccgc cctcatccgc ccgctggaag
    11161 aacagggcat cctattgcac cgcagccgcg aatacctcga aaaccacatt tccgaatttt
    11221 ccatcctcga acacgacggc aacctgtacg gctgtgccgc actcaaaacc tttgccgaag
    11281 ccgattgcgg cgaaatcgcc tgccttgccg tctcgccgca ggcacaggac ggcggttacg
    11341 gcgaacgcct gcttgcccac attatcgata aggcgcgcgg cataggcata agcaggctgt
    11401 tcgcactgtc cacaaatacc ggcgaatggt ttgccgaacg cggctttcag acggcatcgg
    11461 aagacgagct gcccgaaacg cggcgcaaag actaccgcag caacggacga aacccgcata
    11521 ttctggtgcg tcgcctgcac cgctgaccgc aacggaaagc cgccgcagaa atgccgcctg
    11581 aaccccgttt cagacggcat ttccccgatt ataaagacct gcccgaccct caaggacccc
    11641 gatgaaatcc taccccgacc cctaccgcca ttttgaaaac ctcgattccg ccgaaacgca
    11701 aaacttcgct gctgaagcga atgccgaaac gcgcgcgcgt tttttaaaca acgacaaggc
    11761 gcgcgcactt tcagacggca ttttgaatca aatgcaggac acgcggcaga ttccgttttg
    11821 tcaggaacac cgcgcgcgga tgtaccattt ccatcagaat gcggaatatc cgaagggcgt
    11881 gtaccgcatg tgtacggcgg cgacctaccg ttccggctat cccgagtgga aaatcctgtt
    11941 ttcggtggcg gatttcgatg agttgctcgg cgacgatgtg tatttgggcg gcgtgtcgca
    12001 cttggtggag cagcccaacc gcgcgctgct gactttgaac aaatcgggcg gcgatacggc
    12061 gtatacgctg gaagtggatt tggaagcagg ggaattggta gagggcggtt ttcactttcc
    12121 ggcaggcaaa aaccatgtgt cgtggcgcga tgaaaacagc gtgtgggtgt gtccggcttg
    12181 ggacgaacgc cagttgaccg aatcgggcta tccgcgcgaa gtgtggctgg tggaacgcgg
    12241 caagagtttc gaggaaagcc tgccggcgta ccaaatcgat aaaggcgcga tgatggtaaa
    12301 cgcgtggcgt tacctcgatc cgcagggttc gccgattgat ttgattgaag cgtcggacgg
    12361 tttttacacc aagacgtatt tgcaggtgtc gtccgaaggc ggggcgaaac cgttgaacct
    12421 gcctaatgat tgcgatgtgg tcggctatct ggcgggacat cttttgctga cgctgcgcaa
    12481 ggactggcac cgcgcgaacc aaagctatcc gagtggcgcg ttggtggcgg tgaaactgaa
    12541 tcggggcgaa ctcggggcgg cgcagctttt gtttgcgccc gatgaaacgc aggcattgga
    12601 aagcgtggaa acgaccaagc gttttgtggt ggcaagcctg ctggagaatg tacaaggccg
    12661 tctgaaagcg tggcggtttg ccgacagcaa atggcaggaa gccgagttgc cgcacctgcc
    12721 ctccggcgcg ttggaaatga ccgaccaacc gtggggcggc gacgtggttt atcttgccgc
    12781 cagcgatttc accacgccgc tgacgctgtt tgcgctggat ttgaacgtga tggaactgac
    12841 cgtcatgcgc ctccagccgc agcagtttgt ttcagacggc atcgaagtgc ggcagttttg
    12901 ggcggtgtcg tccgacggcg aacgcattcc ttatttccac gtcggcaaaa acgccgcgcc
    12961 cgacacgccg accttagtct atgcttacgg aggtttcggc attcctgaat tgccgcatta
    13021 tctgggcagc gtcggcaaat attggctgga agagggcaat gcctttgtat tggcaaacat
    13081 ccgcggcggc ggagaattcg gcccgcgctg gcatcaggcg gcgcagggaa tcagcaaaca
    13141 caaaagcgtt gatgatttgt tggcagtcgt gcgtgatttg tccgaacgcg gcatgagttc
    13201 gcccaaacac atcggcttgc agggcggcag caacggcggc ctgattaccg ccgccgcctt
    13261 cgtgcgcgaa ccgcaaagca tcggtgcgct ggtgtgcgaa gtaccgctga ccgatatgat
    13321 ccgttatccg ctgctgtccg ccggttcaag ttggacggac gaatacggca atccgcagaa
    13381 atacgaagcc tgcaaacgcc ggctgggcga attgtcgccg tatcacaatc tttcagacgg
    13441 catcgattat ccgcccgcac tcattaccac cagcctcagc gacgaccgcg tccatcccgc
    13501 ccacgcgctc aaattctacg ccaaactgcg cgaaacctcg ccgcaatctt ggctctactc
    13561 gcctgacggc ggcggccata ccggcaacgg cacccaacgc gaatccgccg acaaactcgc
    13621 ctgcgtgttg ctgtttttga aagaattttt gggataaggg cagggggcga cactaccgcc
    13681 gcgaatgaaa aaaggtcgtc tgaaattgct ttttcagacg acctttttta atggttgttt
    13741 caaatcaaaa tatctatgcc gtcggcccat cagcactttt tcacatccga aggcaaaaat
    13801 ccgtaatgcc gcctgaacgc ctcgttgaac cgtcccgcgt ggcggtagcc gcaaaagtgc
    13861 atcgcggctt ggatggtgct gcccgattcg atgagggcga gcgcgtgttc cagccgcagg
    13921 cggcgcaggc gtccggcgac ggtttcgccg gtttgcgctt tgaaatagcg tttcaggtag
    13981 cattcgttca gcccgacgcg gcgggcgatt tcggcgatgg tcagcgggcg ggcgaattcg
    14041 ctgttcaaaa tatcggcggc ttcgtctatg cgccggcggc ggtagccgtt gtcgtggcgg
    14101 cggaaggtga agcgtaataa tcgggcggag agttccagcg cgtcggcttc gtcggcgagc
    14161 aggccgaagc cgtccgattc gaacgggcgt tgcagcaggg ggcaggccgc cgccgtcagt
    14221 gtccccgcgt tttgcgccag ccgttgcagg gcgaatcggc ctatcgtttg cggcgaaaac
    14281 aggcgttcgt ccagtaaacc ttcgtcgtgc cagcggcgca gtttttccag cgaaaaatcc
    14341 aaatgcagcg cgcacatgcc gccgctgtct ggcagcaggg tttcggatac gtccgccagt
    14401 tcgccgcgta ccagccagat ttcgccggca gacgggcggt attccctgcc gcccatttgt
    14461 aaccggttct gccccgatac catgacgaac aaggcgcagt tgtggctgaa attgtggatt
    14521 tcggtgggaa acgcacccgt tccgccgccg cgcatccgtg ataaggtgat gcccgagtcg
    14581 aagcggttga tgcacatttt cagatgcaaa ccgggctgtt ttgcctgcgc aatgagtgcg
    14641 ctgtcggaac agccgtccaa cgcccagccg gatttatcgg agcggacata ggtttggtac
    14701 tggcggtaga tggcggcggt gttcatgatt ggataggaac gagttgtcta acaaatgaat
    14761 taaataggaa ttattaccaa taatcaagca cagggattgg ttggaacgga aaaggtcgtc
    14821 tgaaagggtg tttcagacaa ccttttccgt ttcgggaatt tgttttgccg tatcgggaat
    14881 tttgcgtttt gcggcgtggt ttctgcaggt tgtttgctta ataataaaca ttcttattcg
    14941 tatgcaaagg aaccgcacac cgtgaaaccg cgtttttatt gggcagcctg cgccgtcctg
    15001 ccggccgcct gttcgcccga acctgccgcc gaaaaaactg tatccgccgc atcccaagcc
    15061 gcatccacac ctgtcgccac gctgaccgtg ccgaccgcgc ggggcgatgc cgttgtgccg
    15121 aagaatcccg aacgcgtcgc cgtgtacgac tgggcggcgt tggatacgct gaccgagccg
    15181 ggcgtgaatg tgggcgcaac caccgcgccg gtgcgcgtgg actatttgca gcctgcattt
    15241 gacaaggcgg caacggtggg gacgctgttt gagcccgatt gcgaatccct gcaccgccac
    15301 aatccgcagt ttgtcattac cggcgggccg ggtgcggaag cgtatgaaca gttggcgaaa
    15361 aacgcgacca ccatagattt gacggtggac aacggcaata tccgcaccag cggcgagaag
    15421 cagatggaga ccctgtcgcg gattttcggt aaggaagcgc gcgtggcgga attgaatgcg
    15481 cagattgacg cgctgttcgc ccaaaagcgc gaagccgcca aaggcaaagg acgcgggctg
    15541 gtgctgtcgg ttacaggcaa caaggtgtcc gccttcggca cgcaatcgcg gttggcaagt
    15601 tggatacacg gcgacatcgg cctgccgccc gtggacgaat ctttacgcaa cgaagggcac
    15661 gggcagcccg tttccttcga atacatcaaa gagaaaaacc ccggctggat tttcatcatc
    15721 gaccgcaccg ccgccatcgg gcaggaaggg ccggctgccg tggaagtgtt ggataacgcg
    15781 ctggtatgcg gcacgaacgc ttggaagcgc aagcaaatca tcgtcatgcc tgccgcgaac
    15841 tacattgtcg cgggcggcgc gcggcagttg atacaggcgg cggaacagtt gaaggcggcg
    15901 tttgaaaagg cagaacccgt tgcggcgcag tagttttatt caaaacaaac cacaaggaga
    15961 accccatgca aaacgaaacc atcaacctga aacagcacct tgccgccatc aaagaatact
    16021 ggcagcccga aatcatcaac cgccacgggt tccaattccg cctggtcaaa cttttgggcg
    16081 attacggctg gcacacgcac ggatacagcg acaaagtgct gtttgccgtg gagggcgaca
    16141 tggcggtgga cttcgccgac ggcggcaaca tgacgatacg cgagggcgag atggcggtcg
    16201 tgccgaagtc ggtgtcgcac cgcccgcgtt cggaaaacgg ctgctcggtg gtgctgattg
    16261 atctgcccga cccgtccgag gccgcctgaa aacgaagttt ccgaaggaag ctgagtttct
    16321 gcgaagctaa aagcagccta caccttcaat atgccaaaag catag
//
LOCUS       DAXDXW010000058        16200 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXDXW010000058 DAXDXW010000000
VERSION     DAXDXW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16200
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.059"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..101
                     /locus_tag="ACFB64_001531"
     CDS             <1..101
                     /locus_tag="ACFB64_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HFC1209866.1"
                     /translation="VFACALGALVASEHGATPDISAERIGRFINPV"
     gene            complement(161..718)
                     /locus_tag="ACFB64_001532"
     CDS             complement(161..718)
                     /locus_tag="ACFB64_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC1209867.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQILQAATQVSDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFVPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCEDKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(793..2427)
                     /locus_tag="ACFB64_001533"
     CDS             complement(793..2427)
                     /locus_tag="ACFB64_001533"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC1209868.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELRPFNDT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEALNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEIDKCARATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESAVYYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2490..2828)
                     /locus_tag="ACFB64_001534"
     CDS             complement(2490..2828)
                     /locus_tag="ACFB64_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209869.1"
                     /translation="MIFKCRPNAFQTASAVSAFSGGVLEVVRLCCRRLSVLFNPGVML
                     FVPDCPVGGAAGFFQYEMLCPLFRQGRLQTGSNFAYDVFMSAGRLNGGRKPPRRLLVF
                     LPCFAPLPYN"
     gene            complement(2825..3931)
                     /gene="serC"
                     /locus_tag="ACFB64_001535"
     CDS             complement(2825..3931)
                     /gene="serC"
                     /locus_tag="ACFB64_001535"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC1209870.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLRTAQQEMSDYNGTGFSVMEMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSDGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINDIHPDARSKMNVVFKTASEDLDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4297..4728
                     /gene="rimP"
                     /locus_tag="ACFB64_001536"
     CDS             4297..4728
                     /gene="rimP"
                     /locus_tag="ACFB64_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC1209871.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELAAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIGYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIGGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4756..6258
                     /gene="nusA"
                     /locus_tag="ACFB64_001537"
     CDS             4756..6258
                     /gene="nusA"
                     /locus_tag="ACFB64_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC1209872.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPDTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIAGGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDRLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMNHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGVDADM
                     LLSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6270..9101
                     /gene="infB"
                     /locus_tag="ACFB64_001538"
     CDS             6270..9101
                     /gene="infB"
                     /locus_tag="ACFB64_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC1209873.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVQPEQTAEDAVKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEPKEKAVKPKHERNGKGKDAKKPAKPAAPAVPQPVVSAEEQAQ
                     RDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQAKAAQEAKTGRQRPAKPAE
                     KPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGKGAKGGRDRNNARNGGDERV
                     RGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVADLAHKMAVKGVEVVKALMKM
                     GMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLGEGAEAVEAEALPRPPVVTV
                     MGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVKTPRGVITFLDTPGHEAFTAM
                     RARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPIVVAVNKIDKDTANPERIRQ
                     ELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLEAEVLELTAPVDAPAKGIIV
                     EARLDKGRGAVATLLVQNGTLKKGDMLLAGTAFGKIRAMVDENGKSITEAGPSIPVEI
                     LGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLAKQQAAKLENMFNNMGETQA
                     QSLSVIIKADVQGSYEALAGSLKKLSADEVKVNVLHSGVGGITESDVNLAIASGAFII
                     GFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMSGMLSPEKKEQVTGTVEIRQ
                     VISVSKVGNIAGCMVTDGVVKRDSHIRLIRNNVVIHTGELASLKRYKDDVKEVRMGFE
                     CGLMLKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(9261..10601)
                     /locus_tag="ACFB64_001539"
     CDS             complement(9261..10601)
                     /locus_tag="ACFB64_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HFC1209874.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GGARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAAKLPPELNKARSHWKWSSLNTHRPETSRAERREYAEKIGIRLAGK"
     gene            complement(10675..12015)
                     /locus_tag="ACFB64_001540"
     CDS             complement(10675..12015)
                     /locus_tag="ACFB64_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC1209875.1"
                     /translation="MLNPSRKLVELVRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRSEMIDRDRMLRDTLERVRAGSFWLWVVVASMMFTAGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLATLFLRVKVGRFFSSPATWFRGKGPVNQAVLRLYADQWRQPSV
                     RWKIGATAHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPS
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIVCYGILPRLLAWVVCKILLKTSE
                     NGLDLEKTYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWALMLETEWQDGQ
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRVAQEGRLKDQ"
     gene            12158..12784
                     /locus_tag="ACFB64_001541"
     CDS             12158..12784
                     /locus_tag="ACFB64_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC1209876.1"
                     /translation="MYAGERFNTYSHLSGLILAAAGLMLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYIVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(12849..14267)
                     /locus_tag="ACFB64_001542"
     CDS             complement(12849..14267)
                     /locus_tag="ACFB64_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC1209877.1"
                     /translation="MENILSVLVGTVNRFLWGYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWLSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIVALGFIGNATQANSIASAVTIAFDVPSLAVGIVFAVLAGMVVIGGVNRIANIA
                     RFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMTAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWNLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKQ"
     gene            14594..15322
                     /locus_tag="ACFB64_001543"
     CDS             14594..15322
                     /locus_tag="ACFB64_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC1209878.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIGLRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDELMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15381..15869)
                     /locus_tag="ACFB64_001544"
     CDS             complement(15381..15869)
                     /locus_tag="ACFB64_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC1209879.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCAAVVLACKPKGRVGGLSGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
BASE COUNT         4248 a         4626 c         4134 g         3192 t
ORIGIN      
        1 aggtctttgc ctgcgcactt ggtgcgctgg tcgcctccga acatggcgca acacccgaca
       61 tttccgccga acgcatcggg cgtttcataa acccggttta aaatgcatgg aaaaatgccg
      121 tctgaaagcc tttcagacgg cattttgttt gagattccgt ttaccaatgg ctgacaaacg
      181 cttccaaatc ggtattcttg ggcttatgca cttcctctgt cggcgtgccg accatcatca
      241 gcccgatgat tttatcctta tcctcgcaac cgaaagcctc ccgcaacaat gggctgttga
      301 cccacatccc cgtaatccag acattgtcga atccctgagc cgttgccgcc agttgcagcg
      361 catacgccgc acaacccgcc gtcagcatct gctcccattc cggtttcggc ttaggcacat
      421 cgcggttcgg cacaaacgtt accccgatga ccatcggcgc catattgccc actttttccg
      481 cctttttcat cgcatcgtcg ccgaaattca gttcggcaac cgtttgcttc aacacatcgc
      541 gaaaacgttg caatcctacc tcgccttgaa tcacggtaaa acggaagggg cgcatgttgc
      601 cgtgatcgga aacttgggtt gccgcctgca atatttgttc caactccgcc gcatcggggg
      661 cggggtgctt cagctttttg gaagatcggc ggttcgtcaa taattttaaa gcatccatat
      721 cgttatactc cggtcatcgg tcgggtgttt cggacacata tgccgtgaag gcttcagacg
      781 gcatatccgg catcagcgcg gacggcggca ggctgccaat atatccattt ccttccgata
      841 ggtttggctg ttggaaatgt ccatcagccc caataccgtg ctgaaatagt ggtcgtgcga
      901 atattcgttt tccgccgctt tttgtttgag gcattggaaa tctatgcccc cgtgttggcg
      961 gaaggctttg gaaaaccaca taaccatcgg gatatgcgtc tgtccggaag gcgcgatggc
     1021 gtaaggcgcg gcgtgcaggt acatcccgtt ttcgcccaaa ctttcgccgt ggtcggaaac
     1081 ataatacacc gcgctttcca aatcgtcgcg gttttcaagt ttgcggataa ccttgtcgat
     1141 aaactgatcc acatacaaaa ccgtattgtc gtaagtgttg accaacgtgg cgcgggcgca
     1201 tttgtcgatt tcgttggtat cgcatgtcgg cgtgaatttg cgctcggctt cggtgtagcg
     1261 ttcgtaatac gtcggcccgt ggctgccgat ggtatgcagg attaaaaccg cgtctttatc
     1321 gtttttgttg agggcttcgt cgaacttggt caacaggata ttgtcgaggc actcgccgtt
     1381 gcggcagtat tcgggcaggt tgagcgaagt aacgtcggta ttcggcactt tgccgcacac
     1441 gcccttgcag ccggaatcgt tttccaacca agtaacttcc acgccggcgc gctgcacgat
     1501 gtccagcagg ttgtcttggt gttcggcttt gatttcgtca taatccgtgc ggtcgaaggt
     1561 tgagaacata cacggcaggg agtgcgcggt cgatgtgccg cagcttctga cctgcgggaa
     1621 attgacaatt tcatcgccgc gcgcggcaag cagcggcgta gtttggcggc tgtaaccgtt
     1681 caaaccccag ttggcggcac gcgtagtctc gcccacgacc agcaccacga aacggcgcag
     1741 gctgccggcc ggccggtttt gcacgaccgc catatccaat tgcgtataag gaatattgga
     1801 acgcttccaa tctttgtatt tcgacacgcc cgcgccgatg aaattagacg gcacaatcag
     1861 atgggttact gatttattgt tgcggaaaaa cgaggcgtaa tcctgatatt gcaacatcgc
     1921 gatgcccaac gcgcacaaaa aggaaacggc ggcaagcaca aggcgcgtca aaagctcctt
     1981 ataccaaacg cggtatttga ccttgacggc gatatatgcc agcgcgggca atacgcccaa
     2041 acatacaatc cacagcacat agcccggcgt aatcaggcgc gcgctttccg ccgccgtggt
     2101 ttgcaagaca ttattcaaca tcgacttgtt gaaatagata ttgaaaaata tttcttggta
     2161 agacaccgcc gcactgataa ccaatatcaa tggaatcaac actttatgca caaaaggcag
     2221 ggcaatgacg tgaaaaacga aattacttaa aaaaaacagc accaccggca tcgtatagag
     2281 gaagatgtcc gccccggtgt cgttaaaagg gcgaagttcg acaactttgg caaaaaaggc
     2341 gtaattcaat accagcgaag aatacaggga aagaaaggca atcagcgcgg aagagccgag
     2401 cttcggcctc aggttcggtt ttatcatttg gaatgtgtcg gataagggtt ggaaaaggca
     2461 tccggaatcc ggattattga aaaagattct taattataag gcaacggagc aaagcagggc
     2521 aagaaaacga gtaggcggcg cgggggtttc cgcccgccat tcaaacgtcc ggcagacata
     2581 aaaacatcgt aagcaaaatt cgaaccggtc tgcaaccgcc cctgccggaa aagcgggcaa
     2641 agcatttcat attggaaaaa cccggccgcg ccgccgacgg gacagtccgg cacaaacagc
     2701 atcacgcccg gattgaaaag gacggatagc cgccggcagc acaatcttac cacctccaaa
     2761 acgccgccgg agaacgccga aacagccgat gccgtctgaa acgcattcgg gcggcatttg
     2821 aaaatcaacc gtaacgccgt tggaaatcgc gcataaaatc tgccaaagcc cgcacgcctt
     2881 caagcggcat cgcattataa atgctggcgc gcatcccgcc gacagactta tagcccttca
     2941 acaggcacaa gccctgcaat tcggcttcca gcacaaaacg gcggtccaag tcttcgcttg
     3001 cggttttgaa gaccacgttc attttggagc gcgcatcagg atgaatatcg ttgatgtaaa
     3061 aaccgccgct gccatctatc gtctcataca aggtttgcgc cttcagccga ttgaccgctt
     3121 caattttttt cacaccgccc tgcgcctgca gccagcggaa caccagcccc gacatataaa
     3181 tcgcgtaagt tgacggcgtg ttgtacatac cgtcgcggtt caaatgagaa cggtagttga
     3241 acacatcggg aatatcgttc ggacaacgct cgagcaaatc ctcacgcaca atcaccaccg
     3301 taactcctgc cggcccgatg tttttctgtg cgcctgcgta aatcagtccg tagtcggcaa
     3361 catcaaactc acgcgacaga atctcgctgg acatatcgca caccaacggc ggcatgccgt
     3421 ctgaaaggcg cggcacttca cggtattgca gcccgttgac agtttcattg acggcaaaat
     3481 ggacaaacgc cgaatcgggt gcaacatccc acgtttccac aggcggcagg tcaagatagt
     3541 cgaactgctc gcccccgtgc gccgccaaac ggatttccgt atcggtcaaa cggctcatct
     3601 gttcataggc gatacggctc cagttgcccg ttaccaccgc gtcggcagtg cggaaaccgt
     3661 gtgccagatt catggctgcc atattaaatt gggttgttgc tccgccctga agaaacaata
     3721 tcttatagtt gtcaggcact ttcaaaagct gcctcaaatc ctgttccgca tgatgcagga
     3781 tgctcaaaaa catttccgaa cggtggctca tttccatcac ggaaaaaccc gtaccgttgt
     3841 agtccgacat ttcctgctgc gccgtcctca acacagcttc aggcaacacg gcagggccgg
     3901 cggaaaaatt gtaaatcgga taaagagaca tgatgcagcc ctggttctga acaataaccc
     3961 gcccgatttt aggcttgcag caggctttgt gcaaggatgg gaaatacctg tcctccgccc
     4021 gattccatgc cgcccgaaca cggaaaataa tatcaatata ttgatttaca aacataaaaa
     4081 tcatgctagc gacaaataga tacatttgtt ttgtcaacaa tattcacgat ttcccattac
     4141 aaacctcccg tacacccgct tttttccgtc ccaaaaacac aaaataaatc aacactttca
     4201 tttctccgca aaagcggtta taatcacgcc gatttttcaa cttttgacga aaaatggcca
     4261 cggggccatt tttttattat gtacataggg agcagcatgg atattcaaac catcctcgaa
     4321 aaaaccctgc ccggcctggg atacgaactg gtcgatttcg aactggccgc gcaaggaaca
     4381 ttgcgcgtgt tcatcgacaa agaaggcggc attaccgtcg aagactgcgc caccgtcagc
     4441 aaccacttga gccgcgtctt catggttgaa gacatcggct acaaaaacct ggaaatttcc
     4501 agccccggac tcgaccgccc cttgaaaaaa gccgccgact tcgtgcgctt tgccggtcag
     4561 aatgccaaaa tcaaaacccg cctgccgata ggcggtcaga aaaactttat cggtaaaatc
     4621 gaaggctgcg aaaacgacac tgttaccgtt tccttcgacg gcaaaaccgt gcaaatcgaa
     4681 ttgggcaaca tcgacaaagc ccgcctgcgc cccgaattca aattctaaaa cacagcaata
     4741 ttggagatgt tcaaaatgag tcgtgaaatg ttacagctgg cagaagcact ggcaagcgaa
     4801 aaaaacgttg atgcggaagt cgtcttccaa gcactggaat tcgcactctc taccgccgcc
     4861 aagaaaaagg cagaccgcga acacatggac gtgcgcgtcc aaatcaaccg cgataccggc
     4921 gaataccaaa ccttccgccg ctggctgatt gtggccgatg aagactatac ctatcccgat
     4981 gtcgaaaaaa ccatcgagga aatccaagag gaaatccccg atacgaccat ccaaatcggc
     5041 gaatactacg aagagcagct gcccaacgaa ggcttcggcc gccaagcggc gcaaactgcc
     5101 aaacaaatca tcctgcaacg catccgcgat gccgagcgcg agcagaatct gaacgagttc
     5161 ctcgccgtca aagaagacat cgtgtccggc acggtcaaac gcgtcgaacg ccacggcatc
     5221 atcgtcgaag tcgttgccgg caaactggac gcgctgattc cgcgcgacca aatgattccg
     5281 cgcgaaaact tccgcagcgg cgaccgcatc cgcgccctct tcctgcgcgt cgaagaaatc
     5341 ggcaacaccg gccgcaaaca agtcatcctg agccgtactt ccggcgattt cctcgtcaaa
     5401 ctgtacgcca atgaagtacc tgaaattgca ggcggcatgc ttgaaatccg cgctgtcgcc
     5461 cgcgacccgg gccaacgtgc caaagtcgcc gtcaaagcca acgaccagcg catcgatccg
     5521 caaggcacct gtatcggcgt tcgcggttcg cgtgtcaatg ccgtcagcaa tgaattgtcc
     5581 ggcgagcgca tcgacgttgt cctttggtcg cccgagcctg cccaattcgt catgagcgcg
     5641 ctctcacccg ccgaagtcag ccgcatcgtc atcgacgaag acaaacacgc cgtcgatgtc
     5701 atcgttgccg aggaccggct cgcgctcgcc atcggacgcg gcggtcaaaa cgtgcgcctt
     5761 gcttccgacc tgaccggctg gcagctcaac atcatgactt ccgccgaagc agacgaacgc
     5821 aatgcggcag aagatgccgc catccgccgc ctgtttatga atcacttgaa tgtagatgaa
     5881 gaaaccgccg acgtactggt tcaagaaggt tttgcaacct tggaagaagt cgcctatgtt
     5941 cctgccgccg aactgcttgc cattgaagga tttgacgaag aaatcgtcga tatgctccgc
     6001 aaccgtgccc gcgatgccat cctgaccatg gcgattgctg ccgaagaaaa actgggcgaa
     6061 gtgtccgacg atatgcgtaa cctcgaaggc gtagatgccg atatgctcct cagccttgcc
     6121 gaagcaggca ttaccacccg cgacgacttg gcagagcttg ccgtggacga actgattgaa
     6181 atcaccggtg taaacgaaga aactgcaaaa gccgtcatcc tgaccgcacg cgaacactgg
     6241 tttaccgaag acaaataaag ggggtacaga tgagtaacac aaccgtagaa caatttgccg
     6301 ccgagctgaa acgccccgtc gaagacctgt tgaaacagtt gaaagaagcc ggcgtcagca
     6361 aaaacagcgg cagcgattcc ctgacgctgg acgacaaaca gcttctgaac gcctacctga
     6421 ccaagaaaaa cggcagcaac ggcggcacca tcagcatccg ccgcaccaaa accgaagtca
     6481 gcaccgttga cggcgtaaaa gtcgaaacac gcaaacgcgg acgcactgtc aacattcctt
     6541 ctgccgaaga attggcagca caagtaaaag ccgcccaaac ccaagccgca cctgttcagc
     6601 cggagcagac ggcagaagac gcggtaaaag cccgagccga agctgccgca cgcgcagaag
     6661 cccgtgccaa ggcagaagcg gaagcggcaa aactgaaagc ggcaaaagca ggcaacaaag
     6721 ccaaacctgc cgcgcagaaa cctaccgaag caaaagccga aaccgcaccc gttgcggcgg
     6781 aaaccaaacc cgccgagccc aaagaaaaag ccgtcaagcc gaaacacgag cgaaacggca
     6841 aaggcaaaga tgccaaaaaa ccggcgaaac ctgccgcacc tgccgtgccg caacccgtgg
     6901 tcagcgcgga agaacaggcg caacgcgacg aagaggcgcg ccgcgccgcc gcattgcgcg
     6961 cccaccagga agccctgttg aaagagaaac aggaacgcca ggcacgtcgc gaagccatga
     7021 aacaacaggc agaacaacag gcaaaagccg cacaggaagc caaaaccggc agacagcgtc
     7081 ccgccaaacc tgccgagaaa ccgcaggcag ccgcaccggc cgtcgaaaac aaacctgtca
     7141 atccggcaaa agcgaaaaaa gaagaccgcc gcaaccgcga tgacgaaggt caaggccgca
     7201 acgccaaagg caaaggtgca aaaggcgggc gtgaccgcaa caatgcccgc aacggcggcg
     7261 acgagcgcgt acgcggcggc aagaaaggca aaaaactcaa actcgagccg aaccaacacg
     7321 ccttccaagc accgaccgaa cccgtcgtac acgaggtttt ggttcccgaa accattaccg
     7381 ttgccgattt ggcgcacaaa atggcggtca aaggcgtgga agtggtcaaa gccctgatga
     7441 agatgggcat gatggttacc atcaaccaat ccatcgacca agacaccgcc ctgattgtcg
     7501 tcgaagaact cggacacatc ggcaaacccg ccgccgccga cgatcccgaa gccttcttgg
     7561 gcgagggcgc ggaagcggtg gaagccgaag cattgccgcg tccgcccgtc gttaccgtca
     7621 tgggccacgt cgaccacggc aaaacctcgc tgctggacta catccgccgc gccaaagtgg
     7681 tacagggcga agcgggcggc attacgcagc acatcggcgc gtaccacgtc aaaaccccgc
     7741 gcggcgtgat taccttcttg gacaccccgg gccacgaagc ctttaccgct atgcgcgcgc
     7801 gcggcgcgaa agcaaccgat atcgtgattc ttgtggtcgc cgccgacgac ggcgtgatgc
     7861 cgcaaaccat cgaggcaatt gcccacgcta aagcggcagg cgttccgatt gtggttgccg
     7921 tcaataaaat cgataaagac actgccaacc ccgaacgcat ccgtcaggaa ctgacccaac
     7981 acgaagtcat ccccgacgat tggggcggca cggttcaatt catcgacgtt tccgcgaaaa
     8041 aaggaacgaa catcgacgcg ctgctcgaag ccgtattgct cgaagccgaa gtattggaac
     8101 tgaccgcacc tgtcgatgcg cccgccaaag gcatcatcgt cgaggcgcgt ttggacaaag
     8161 gacgcggcgc ggttgccaca ttgctggttc aaaacggtac gctgaaaaaa ggcgacatgc
     8221 tgctggccgg tacggcattc ggcaaaatcc gcgcgatggt cgatgaaaac ggcaaatcca
     8281 ttaccgaagc cggcccgtcc atccccgtcg aaatcctcgg cttgtccgac gtaccgaatg
     8341 cgggtgaaga cgcgatggta ttggcggacg agaaaaaagc gcgcgaaatc gccctcttcc
     8401 gccaaggcaa ataccgcgac gtgcgccttg ccaaacagca ggcggcgaag ctggaaaata
     8461 tgttcaacaa tatgggcgaa acccaggccc aatctttgtc ggtcatcatc aaggcagacg
     8521 ttcagggctc ttacgaggct ttggcgggca gcctgaaaaa actgtccgcc gacgaggtga
     8581 aagtgaacgt gttgcacagc ggcgtgggcg gcattaccga atcggatgtc aaccttgcca
     8641 tcgcttcggg cgcgttcatt atcggcttta acgtgcgtgc agatgcctct tcgcgcaaac
     8701 ttgccgaaaa tgaaaacgtg gaaatccgct actacaacat catctacgat gccatcgacg
     8761 acgtgaaggc ggcgatgagc ggcatgcttt ctcccgaaaa gaaagaacag gttaccggta
     8821 cggtcgaaat ccgtcaggtc atctccgttt ccaaggtcgg caacattgca ggctgtatgg
     8881 tcaccgacgg cgtggtcaaa cgcgattccc atatccgcct catccgcaac aacgtggtca
     8941 tccacacggg cgaactggct tcgttgaaac gctataaaga cgacgtaaaa gaagtccgca
     9001 tgggcttcga gtgcggtctg atgctcaaag gctacaacga aatcatggaa ggcgaccaac
     9061 tggaatgctt cgacatcgtc gaagttgccc gcaccctgta attcctttgc aaataaaatg
     9121 ccgtctgaag cgttcagacg gcaacgaaac gggttctgtg tcatacagaa cccgtttttt
     9181 gtcgcaaatc ggcttcagcg accctcctgc cttatcccga tttgaatctg acttgccata
     9241 caaacaggct tcagacggca ttatttgccc gccaaacgta tcccgatctt ctccgcatat
     9301 tccctgcgtt cggcgcggct ggtttccggg cggtgcgtat tgagcgacga ccatttccaa
     9361 tgactgcggg ctttgttgag ttcgggcggg agtttggcgg catcccacgg ggctttgcgg
     9421 ctgtgcagct cgatatccga ctgtgccgcg tgtccgcgcg tttgcaggac gtggagcaga
     9481 tcgagggcgc gggcggcgag cagggtcagg gtttcagggt cggtgtgcag ggtttgacgg
     9541 ccggcgagtt tgtcggaaat ggtgcgggta ttgggcagga tgccgcccaa aaagccgccg
     9601 attgccgtac ccaatccgag cgagccgccg agtgtggcga tgtccagccc caagccgatg
     9661 agcgcgccgg ttgccgcgcc cgtgccggtg cggatgccgt attgtttgag caattcgctg
     9721 tcgaacgggt cttggcggaa ggcttgcggc atccagtcgc cgccgtcgat ttcgctgtgg
     9781 tagaaacggt aaagggcaaa cagccgctgc tgcatctgcc gttcgagttg gcgtatttcc
     9841 gcctgcatgg tttgcagcac ggtggcggta tcctcgtttt cgtccacttc ctgcctgaag
     9901 gcggcggcat cgagcaggaa atcggcgatt tcgcggcgcg ctccgccgtc cagccgctgc
     9961 cattcgcgcc ggcgcatggc tgtcaggcgg tcaagtgtgc tgcgttcggg caacatggtg
    10021 gcgaggtttt cccacaggcg caattcgcct tcaaaatcaa aggcgacggt gtcgaaccct
    10081 gcgaaaacgt gcaggtttct cctcgccagc atggttgtcc acgattcggg aagctgtccg
    10141 ccggtaaagt tgaacacggg cataaccggt ttggcacacc atgaaaggat ggtcaattca
    10201 tcccgatatt tgtcgaggac gggttcgcgc gcgtcgatga cgtacattgc catatcgctt
    10261 tgcaaaacct gccgcagcac tttggcttct tggttgaaat cgtgatgcgc gccgtggctg
    10321 ccgagaaact gttgcagccg ctcgatgccg tctgaacgat tgtccgtatg gttttccagc
    10381 cattccaaaa caccgcccgc gtcttcgagt ccgggcgtgt catacaggaa aaccaacatg
    10441 tccgcgccgt cgctgatggc ggcttcttcg acatggcgtg tggtggacgg tgcgtttctg
    10501 acttcgccga agccgctgtc gcgcaacagg gtgcgcagga gcgaggtttt gccggtgttg
    10561 gtgtgtccca cgacggcgag ggaaaggggt tgtttgttca tgatgttttt gaagaatgga
    10621 ttttcagacg gtcttttttc agaatggcgg cttaacagaa catttcaagt gagtttattg
    10681 gtctttcaaa cggccttcct gcgccaccct gtcaggctca agccacgccg cgccgcattc
    10741 ggtcagcgcg ttacgccaat gttccagctt ttccgaaagg tcgtctgaaa gcccctgttc
    10801 cgccaaaagc tgcaccaccg cgccgccctg cgccgcttcc gaaagccgca caatctgccg
    10861 cagcacgccc cggtccggca cagtttgggc gcgtacgccg ataagcagtt gcgccggttt
    10921 ctgcttcagc tctgtctcca gcgcggcaac ctgttcccga ttggcggcaa cgcccttatc
    10981 cagccattcc tgcgccagcc tgccctcgaa ccattggccg tcctgccact cggtctccag
    11041 catgagcgcc catttcggcg catcgttcaa gacgattttc ggcgaaacgg cggacacggt
    11101 ttcccgacgc gtatccgcat cggtgatttt gttctgccag cggcggatga ccgcctgata
    11161 ataggttttt tccaaatcca atccgttttc gcttgttttc aaaaggattt tacacactac
    11221 ccaagccaag aggcgcggca ggatgccgta gcagacgata ctgccgacca gcagccccga
    11281 ccaagcccgc gcatcggcaa tattgccgtt cagacgacct tcgatgaccg cccgcgcatc
    11341 ggggacaggg aaaccgagtt tcgacggcag ccatgccaac atttccaccg cgcgtaccga
    11401 agcggcattg ctcaacagcg tgctttccca gttgaacgta tattgccgca ccaaaagcag
    11461 cagcaatacc gacaccagca ttccgagcag cgtgcagagc cacaagctgt gcgccgttgc
    11521 gcctattttc catcgtaccg aaggttgccg ccactggtcc gcatacagcc gcaacaccgc
    11581 ctgatttaca gggcctttgc cccgaaacca cgtcgccgga ctgctgaaaa accgccccac
    11641 tttcacgcgc aggaacaacg ttgccaacca tactgccagc atcagcgtat tcatgcccaa
    11701 cactcccgcc aaaactaaaa agaaattcag cccctgattg tccatcagaa gataagtgcc
    11761 tgaaaatccg gcggtaaaca tcatcgatgc caccaccacc cataaccaga acgaccccgc
    11821 acgcacacgt tccaacgtgt cccgcagcat acggtccctg tcgatcatct ccgaccgacg
    11881 gatgattttt tcctccgtac tgccgtccac gcggcgcaaa gcctccgtcg cctgcacggg
    11941 atcgccgctg aaaataaaac cgccttcatc caaaatacgg accagctcaa ccagttttcg
    12001 ggatggattc aacataaaat gccgtctgaa aataaaaaac aaattttaac acacgcattt
    12061 tcaagaatat tcacagtgta ggcaaagagt aaatcccaca cagaggcaaa agtatcggcg
    12121 taaactgact gcctctactt tcccgaaaga ttgtgcgatg tatgcaggcg aacgcttcaa
    12181 tacttacagc catttgagcg gtttgattct ggcggcggca ggtttgatgc tgatgctgct
    12241 gaaaaccata ggacacgggg acggctaccg tatcttcagc gtatcggttt acggcatcag
    12301 ccttcttctg ctctatttga gttcctcgct gtaccacgga attgcagccg gaaaactgaa
    12361 aagcattttg aaaaaaaccg accactgcat gatttatgtg ctgattgccg gaagctacac
    12421 accgtttgca ctggtttctt tgagaaacgg gccgggctgg acggtatttt cactgtcctg
    12481 gctgctggcg gctgcaggaa tcgcacaaga actcaccatc ggacggaaaa gcgaaaaacg
    12541 tctgctgtct attgcgattt atatcgtaat gggctggatg gtcttggcgg taatgaaatc
    12601 cctgacagcc tcactcccgc cggcaggact ggcttggctg gcggcaggcg gtatgctgta
    12661 cagcgtcggc atttactggt ttgtaaacga tgaaaaaatc cgacacgggc acggaatctg
    12721 gcatctgttc gtattgggcg gcagcataac ccaatttgtc agcgtgtacg gttatgtaat
    12781 ctgaatgccg tctgaaacac aaaaacctcc caaacctgaa ggcagggagg ttttctcttt
    12841 gccgggcatt attgtttatc gtggaactcg tggaattcat actgatagga caaatcccga
    12901 cccgcttttt tctgtgccaa ataatcatca taaatggcgc ggatttcctt acgcagcaag
    12961 aacagggcaa tcaggttggg gataaccata aagccgttga acatatcgga cagattccaa
    13021 accaaatcga ctttgccgag cgtacccaaa acgatagcaa gcagaaccaa tgcgcgatag
    13081 atgcccaagt gtcttcccct gaaaagaaaa cggatgttgg actcgccgaa ataataccag
    13141 ccgataatgg tcgtaaaggc gaagaaggtc agacacacgg caagcaattg cgaaccgaag
    13201 cccggaaatg ccttgttaaa ggcaaattga gtaaccgccg cgccctgttc gcccgaaagg
    13261 ttggcatcgg tcagcaggat aatcaatgcc gtagccgtac ataccaaaat cgtatcgata
    13321 aatacaccga caaatgccgt cataccttgc tgaacaggat gcttcacatc cgcagtcgcg
    13381 tgggcgtgcg gagtcgaacc catacctgct tcgttggaaa acagaccgcg cgccacgccg
    13441 aaacgtatcg cttcacgcat accgataccc gcagcaccgc ccaaaacggc ctcgggattg
    13501 aaggcggcgg taaagatgtg gttgaacatc ggcacaatat ggtcggaaaa ttcaaacagg
    13561 ataacgacgg cgcacaaaat ataaacaacc gccataaacg gcacgacaaa ccgggcaata
    13621 ttggcaatac ggttcacgcc gccaatcaca accatgcccg caaggacggc aaacacaata
    13681 ccgactgcca aagaaggcac atcaaatgca atggtaacgg cagaagcgat ggaatttgcc
    13741 tgagtcgcat tgccgataaa gcccaacgcg acaatcagcg cgatggagaa aaagcccgac
    13801 aggaaacgcg ccgcgcccct gccgattttc ggagtcagac cgtgcgtaat ataaaacgcc
    13861 ggcccgccga tgtatttgcc gtggctgacg acgcggtatt tctgcgccag cagtgcttcc
    13921 gcaaaaatcg tggacatccc caaaacggca gaaagccaca tccaaaaaat cgcacccggc
    13981 ccgcctgcgg taatggcggt cgccacaccg gcgacgttgc ccgtaccgat ttgcgcggat
    14041 atagcaaccg ccaacgcctg aaactgcgat aaggacttat cgtctttatc acctttggca
    14101 aacaagccgc cgaatacgga tttgaatccc gcgcccagct tggtaatctg cggcgcacca
    14161 agatacagcg taaaaaacag gccgataccc aaaagcgcat aaatcagcag gtagccccaa
    14221 agaaaccgat tgactgtacc caccagaaca gacaatatat tttccataaa ataaacctta
    14281 tcttacgatt aaaatgactg ttttccaaaa gacattccaa taaggaaaca cggcgagcag
    14341 accgtatttg ccgcaacaga tgccttaaat tgtcaataat ccgagagaag ctgtgccagc
    14401 ataccgtaaa atatcgttaa ttaaaatatt tctttatttt taagcggaaa gcggaggaaa
    14461 tcgttttcag acggcatcga caacggcacg gcataaaaca ggatattttg ggtacttgca
    14521 acttatgtta aaatgccgac cgtaaaaaat ctgacaaaaa cagattaatt atttgaaata
    14581 agaaaggaaa tttatggcag gccatagcaa gtgggcgaat atccagcata aaaaagcccg
    14641 tcaggatgcc aaacgcggca aaatcttcac ccgtttaatc aaagaaatca ccgttgcggc
    14701 gcgtatgggc ggcggcgatc ccggcgcaaa tccgcgcctg cgtctggctt tggaaaaagc
    14761 agccgaaaac aatatgccca aagacaatgt gcaacgcgcc atcgacaaag gtacgggtaa
    14821 cttggaaggc gtggaataca tcgggttgcg ctacgaaggc tacggcatcg gcggcgcagc
    14881 tttgatggtg gactgcctga ccgacaacaa aacccgcacc gttgcggacg tacgccacgc
    14941 atttaccaaa aacggcggca acttgggtac cgacggctgc gtggcgttca acttcgtgca
    15001 tcagggctat ttggtattcg aacccggcgt tgacgaagac gagctgatgg aagcggcttt
    15061 ggaagccggt gcggaagacg tggttaccaa cgacgacggt tccatcgaag tcattaccgc
    15121 gccaaatgat tgggcgggcg taaaatccgc tttggaggcg gcaggttaca aatccgttga
    15181 cggcgacgtt acgatgcgcg cccaaaacga aaccgaactc tccggcgacg atgccgtcaa
    15241 aatgcaaaaa ctgattgacg cgctggaaga cttggacgac gtgcaagacg tttacacttc
    15301 cgccgtattg aatctggact gatacgcagc acagcagaca tacaacaaaa tgccgtctga
    15361 acctttcaga cggcattgat ttatttagcc cttgcccacg cccaccaaac cgtcaggaca
    15421 accgccaaaa aatacgccgc gctccaaaca ggcaaagcaa cccctaacag ataatccggt
    15481 tcggcacaat ttccgaaccc gcgcacaaca ggctcgaacc aatcaaacaa aggccagccc
    15541 ttcaatcgaa acgtccacgg cgcgccgcac gaaggagccg tacccggcgg cagcgactgc
    15601 aaccacaact gatatgccgc aacagaaata cccgtaacgg cgggaatact gataaacact
    15661 gcgcctgaca gtccgcccac cctgcccttg ggcttacacg ccagcacaac tgccgcacac
    15721 aatgcggttg ccaaaacaca taaccgctga ctgatgcaca aaacgcaagg ctccataccc
    15781 aaaacatact gtgccgccaa agaaccggca aatgcacaga ccgaaacggc aaacagcagc
    15841 caaacggctt ttctaaataa cggggtcatt ttctcaacac accaatcaaa ataccgatat
    15901 gccgattttg ctggatatat cccgagatgg caagggacga atcgacaccg gcacaggtgc
    15961 ggacgatttc ggttttcccg atatgcacaa aacctgacaa aggacaaaac ggcgatttgt
    16021 ccgcacaatg cccgaaaaaa tacatccgga ggatttgaat ttgaatttcg gcaaatttag
    16081 cgaatcaaaa gcaacagccg ataaaaaaat caaaagctta tttcaatgtt tcaacacaca
    16141 ggacgacaca taaagcaccg ccctatgtgc cgtcctgatt tggaaggggt tacacccctc
//
LOCUS       DAXDXW010000059        16132 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXDXW010000059 DAXDXW010000000
VERSION     DAXDXW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16132
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.060"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            158..2402
                     /locus_tag="ACFB64_001545"
                     /pseudo
     CDS             158..2402
                     /locus_tag="ACFB64_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            2527..4077
                     /locus_tag="ACFB64_001546"
     CDS             2527..4077
                     /locus_tag="ACFB64_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC1209880.1"
                     /translation="MKKPLISVAAVLLGVALGTPYYLGVKAEESLTQQQKILQKTGFL
                     TVESHQYDRGWFTSTETTVIRLKPELLHNAQKYLPDNLKIVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGETVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGRFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGDASGLFTHDPVLNIKIFRFTLPQGKIDVGGKIMFK
                     GMKKEDLNQLGLMLKKTEANIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEARASIAD
                     INETLRLMVDSTVQSMAREKYLTLDGNQIDTVISLKNNALKLNGKTLQNEPDPDFDEG
                     DMVSGQPH"
     gene            complement(4501..6285)
                     /gene="lpdA"
                     /locus_tag="ACFB64_001547"
     CDS             complement(4501..6285)
                     /gene="lpdA"
                     /locus_tag="ACFB64_001547"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC1209881.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVIKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGSAADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGVCTDLPPQKKK"
     gene            complement(6303..6737)
                     /locus_tag="ACFB64_001548"
     CDS             complement(6303..6737)
                     /locus_tag="ACFB64_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209882.1"
                     /translation="MSYLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKAT
                     HGPSVKNAVYAHQIETDLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQY
                     SIFEYEVEHPENPDEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(6838..8400)
                     /gene="aceF"
                     /locus_tag="ACFB64_001549"
     CDS             complement(6838..8400)
                     /gene="aceF"
                     /locus_tag="ACFB64_001549"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC1209883.1"
                     /translation="MSIVEIKVPDIGGHESVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAAEAQPAPAAAGG
                     ATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVKAV
                     FLKVGDKVSEGSAIIEVETAGSAAAAPAPAAQAAAPAAVPTSASPAAAKIDEAAFAKA
                     HAGPSARKLARELGVDLGQVKGSGLKGRIMGDDIKAFVKSVMQGGAAKPAAAGASLGG
                     GLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHEEADMTELEEF
                     RKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFNIGFAADT
                     PNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTISSLGGIGGTG
                     FTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDGAAGMRFTVFL
                     ANLLKDFRRITL"
     gene            complement(8550..11213)
                     /gene="aceE"
                     /locus_tag="ACFB64_001550"
     CDS             complement(8550..11213)
                     /gene="aceE"
                     /locus_tag="ACFB64_001550"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC1209884.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLLKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSDFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIETERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            11912..13624
                     /locus_tag="ACFB64_001551"
     CDS             11912..13624
                     /locus_tag="ACFB64_001551"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC1209885.1"
                     /translation="MKASQFFISTLKEAPAEAAFASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHER
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQATYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGELVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFTGKVYADFATEKGADWVIGANEDDYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRGKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADRIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRGNEAQAVAIGEIVARVTA
                     SLNA"
     gene            13687..14343
                     /locus_tag="ACFB64_001552"
     CDS             13687..14343
                     /locus_tag="ACFB64_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC1209886.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHVEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLIGRDSDGTGEGEDILGCVAGYGVGLDLTA
                     RDIQCRLKAQGLPWLKAKGFRHSACVSDFAAADRIGNQDKVLFSLKQNGVLKQRGETG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVCASFTIGC
                     "
     gene            complement(14353..14808)
                     /gene="ruvX"
                     /locus_tag="ACFB64_001553"
     CDS             complement(14353..14808)
                     /gene="ruvX"
                     /locus_tag="ACFB64_001553"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC1209887.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLATVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHADGTEHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVLGKKRKSVLDQVAAQAILHGFLEGGPAECFNGREG"
     gene            complement(14801..15349)
                     /locus_tag="ACFB64_001554"
     CDS             complement(14801..15349)
                     /locus_tag="ACFB64_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC1209888.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDGIALTS
                     SRDVLENISREGAVDKALISIGYSSWSKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(15351..15911)
                     /locus_tag="ACFB64_001555"
     CDS             complement(15351..15911)
                     /locus_tag="ACFB64_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC1209889.1"
                     /translation="MNRLLLLSAAVLPTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKRGTGFAFKSRQIVRYYDPKRKAFAYLVYSDKIVQGSPKNSLSAVSCFGSGIPQTDG
                     VQADTSGKLLAGACIISNPIKNPDKR"
BASE COUNT         3825 a         4009 c         4321 g         3977 t
ORIGIN      
        1 cacaaaaata aaccaatatt taacagtcgg atagctccgc ctgattaagt ttttcggtaa
       61 tagttgtgat tatcaaatat aattgccgcc cgaaatatcc gataagtttg tttgttttat
      121 ctttaattcc ccaatactta aaaaagagag cgttttcatg aacaaaaaac ttgccttaat
      181 gccgctgttg attttgagcg cgttttcatc tgccgccgac aatactccgc agcatggcga
      241 actcggacag gttcatgtcc gcgccgatgc caagcgcgtc aaagccgcgc attcttactc
      301 catcgccagc gatggcgact tgcgcgaccg cgtaaatttg ggcgtattgg gtaaagccaa
      361 cgcgtttacc gcgccgatta ccgtcgtcaa ctacgacgaa caagccttca acaataccga
      421 ggcgcgtact ttagtggatg ccgtagccaa aaaagacgct tccgtttgag agtttggcgg
      481 cgaaagcaac acgctgaccg gtctgtattt ccgtggttat cagcttgatg cgcgccaatt
      541 cagcgtaaac ggcttggcag gaatgtatgg cacgcaaggc acggcgagcg tgcaagtcgg
      601 cttcgcacaa ctgattaaag gcgcgtccac cgccgtaaac ggcataccct gaaggcgcgg
      661 tatccggttc cgtcaacatc gaaaccaaaa aagccgccga cgaaggcaac cgcaaaatcg
      721 gtttggcccg gttcggcaac aaccgcgccc aaggtaggtt cgacttaggg caacgcttcg
      781 gcgaaaacaa agcattcggc gtgcgtgcca acggcaaact gcgccacggc gacaccccgc
      841 gccacggtta cagagaagac aacaaagaat ttgccttaaa tgccgattat cgcggcgaaa
      901 aactgcgcgt gacgttcgac tccatctacg cgaaacgcaa aatcaacggc ggccgcgcgc
      961 gtatgaagga tatccaaaac gccggcggac gcttgttcga cgcgcccgac ggcaaaatca
     1021 acctgctgcc ttcttggaac tggcaaaata ccgtcggcga aaccaatatg ctgacgtttg
     1081 aatgggatgc gtttgacaat acctaaatta ccggcggtat cggctacaac aaagcgcgtt
     1141 attacggtac gctgatttcc ccaaccgttt gcggtacaag cggtgcgagc agccaaacca
     1201 aaacctgcat ggctgccaac caataccaca ccggcacggc gcgcctgacc gaccaatatt
     1261 tccgtacttt gagtatgaac ctgaccgcac gcggcgaatt tgaaaccggc ccggtcacgc
     1321 ataactggag tacggctttc gaccgcgtta tccgccagcg gaaaaccata aacggcagcg
     1381 ggaacggcaa cagcaagata gaagtgaagg caaacgaaaa tattgcgaat caattggcat
     1441 cgttcaaagc cgactatccc aattcttggg cgaactcggc caacttggat gccaatatca
     1501 aagtcaacag cttggctttg tccgatactt tgggattcgt tgataacaaa taccctctga
     1561 ctttgggcgg acgtttccaa gcggttgaat acactgataa gaagaaatca cagagcggcg
     1621 atgccaaacg tttcagcccg atgctgatgg cggcgtgggt gccgcaacct gatttggtcg
     1681 tgtgcggcaa ctatatggaa gacttggagc gtgccgatat caaaaccgat gattccggcg
     1741 aaacgacaat ggcgaagccg cgcgtcagcc gccagtttga aatcggcgtg cgtaaaaact
     1801 ggggcgattt tgtaaccacg ttgaacgcgt tccaaatcaa acgcccgggc tactggcgcg
     1861 gcaataccaa aaaaggtact gattttgcag catataaagc agcaggaggc gcagccggcg
     1921 acgagcaagg tatggaacgc aatcgcggta ttgagttcaa tgcctatgcc aacctgttga
     1981 acaaaaccct gcgcccgact ttcggcgtga tgtatctgca atcgtccgtg aagaattatc
     2041 cgaatgcgcg cgatatgctg gttaacggcg tacaagtcgc caacccgcgc gtgattgtca
     2101 aaacaggcgt tgaacgggat acaccgtttg ccaaaggttt gagcttgaac ggcaatgttt
     2161 cgtatttcgg caagtcttat caagacacgc aaaaacaata cgccttcccg tcctatacct
     2221 tggttgacgt aggcgcgcgc tacaaaacca agctgggcaa aaacaccctg accgtcagca
     2281 gctcggtaga aaacctgttc aacaaaaact actggcaggt acagcgcgga caatttgacc
     2341 gtagtttcgc cgtcgtcggt ctgccgcgta cttactggct gaaggcggaa ttggatttct
     2401 aagctgcctc cgtttccgaa aatgccgtcc gaacaggttt cagacggcat ttcttttaaa
     2461 gaaatatggc gggatatgct taaataatgc tattctagta cctttgaaac ctgcttgttt
     2521 gaagtgatga aaaaaccttt gatttcagtt gcggcagtat tgctcggcgt tgctttgggt
     2581 acaccttatt atttgggtgt caaagcagaa gaaagtctga cgcagcagca aaaaatattg
     2641 cagaaaacgg gctttttgac cgtcgaatcg caccagtatg atcgaggctg gtttacctct
     2701 acggaaacga cggtcatccg tctgaaaccc gagttgctgc ataatgcgca gaaatacctg
     2761 ccggataact tgaaaatagt gttggaacag ccggttacgc tggtaaacca tatcacgcac
     2821 ggccctttcg ccggcggatt cggcacgcag gcgcacattg aaaccgagtt caaatacgcg
     2881 cctgaaacgg aaaaagtttt ggaacgcttt tttgggaaac aagttccggt ttcccttgcc
     2941 aataccgttt atttcaacgg cagcggtaaa atggaagtca gtgttcccgc tttcgattat
     3001 gaagaactgt cgggcatcag gctgcactgg gaaggcctga cgggggaaac ggtttatcaa
     3061 aaaggtttca aaagctaccg caacagctat gatgcgccct tgttcaaaat caagctggca
     3121 gacaaaggcg atgccgcgtt tgaaaaagcg catttcgatt cggaaacttc agacggcatc
     3181 aatccgcttg ctttgggcag cagcaatctg actttggaaa aattttcgct cgaatggaaa
     3241 gagggtgtcg attacaacgt caaattgaac gaactggtca acctcgttac cgatttgcag
     3301 atcggcgcgt ttatcaatcc caacggcagc atcgcacctt ccaaaatcga agtcggcaag
     3361 ctggcttttt caaccaagac cggggaatcg ggcgcgttta tcgacagcga agggcggttc
     3421 cgtttcgata cgttggtgta cggcgatgaa aaatacggcc cgctggacat ccatatcgct
     3481 gccgaacacc tcgatgcttc tgccttaacc gtattgaaac gcaagtttgc acaaatttct
     3541 gccaaaaaaa tgactgagga acaaatccgc aatgatttga ttgcggcagt caaaggcgat
     3601 gcttccggat tatttaccca tgacccggta ctaaatatca aaattttccg tttcaccctg
     3661 cctcagggaa aaattgatgt gggcggaaaa atcatgttta aaggcatgaa gaaggaagat
     3721 ttgaaccaat tgggactgat gttaaagaaa accgaggcaa acatcagaat gagtattcct
     3781 caaaaaatgt tggaagattt ggcggtaagt caggctggaa atattttcag tgtaaatgcc
     3841 gaagatgagg cggaagccag agcaagcatt gccgatatta atgaaacatt gcgcctgatg
     3901 gtggacagta cggtccaaag tatggcaagg gaaaaatatc ttactttaga cggtaatcag
     3961 attgatacgg tcatttccct taaaaacaac gccctgaagt taaacgggaa aacgctgcaa
     4021 aatgaacccg atcctgattt tgacgaggga gatatggttt ccggccagcc gcattaatgc
     4081 cgtttatgcc aaatgccgtc tgaaatgttc agacggcatt tttgtcggtc tcgaaatagg
     4141 atggattttc atgtttcaaa aacaattgat tgtctctaat ccgatatcag gacacgcttc
     4201 caatgtttag atatggaatt cggacgggat aggggaacta ttgaaatcca gttgaaatct
     4261 gttattggaa agcatggagg aaaaataaaa ggctgtattt taatatagta ttgataggca
     4321 tgcttagctt caaggtattc aatagtgtag gtgtctgcct aactggaaaa taatcgtctt
     4381 ttcccttgca accagttaaa attgacagat ggttaaaaca cagccaaata aaaatgccgc
     4441 ctgaagggtt cagacggcat ttgttcggat aaaccttatc ggctgcttag agcatcaggt
     4501 ttattttttc ttttgaggag gcaggtcggt acatacaccc aaagccactt ccgcagccat
     4561 accgatggat tcgcccaagg tcgggtgcgg gtggatggtt ttgccgatgt ctgccgcgtc
     4621 gcagcccatt tcgatggcaa ggcagacttc gccgatcatg tcgccaccgt tcggaccgac
     4681 aatgccgccg ccgatgatgc ggccggtttc ggcatcgaaa atcagcttgg taaagccgtt
     4741 gtcgcaaccg ttggcaatcg cacgaccgga agccgcccat gggaagttgg ctttggtgat
     4801 tttgcggcct gatgctttgg cagacaattc ggtttcaccg acccatgcca cttcggggga
     4861 agtgtaggca acgccgggaa taacgcgtgc gtcgaagtag gctttgtggc cggcacagtt
     4921 ttcagcggca acgtgacctt cgtgaacggc tttgtgtgcc aacatcggtt gaccgacgat
     4981 atcgccgata gcgtagatgt gcggtacgtt ggtgcgcatt tgtttgtcaa cttcgatgaa
     5041 gccacgatcg gtaacggcaa caccggcttt ttctgcgctg atgagtttgc cgttaggcgc
     5101 acggcctgcc gcgaccagta cggcatcgta acgttgcggc tctttaggtg catttgcacc
     5161 ttcaaaggta acgtaaacgc cgtcttcttt cggctcgact gcaacggttt tggtgttgac
     5221 cataatgttg tcaaaacggt attcgttttg tttttgccat actttaacca agtcgcggtc
     5281 tgcgccttgc atcaggccgt ccatcatttc aaccacatcc aggcgagaac ccagcgtgct
     5341 gtaaaccgta cccatctcga ggccgataat gccgccgccg ataatcagca gtttgcccgg
     5401 tacttctttc agtgccagtg cgccgctgga atcgatgatg cgcggatctt caggaatgaa
     5461 aggcagtttg gttacgcggc tgcctgctgc aatgatacag tttttgaagg caacgatttt
     5521 tttctcgccg gtaggggctg cctgttcgta cgcgtcgccg gcagtcagcg acacttccaa
     5581 gtggtgagga tccaagaatt gcccgtcgcc ttggataacg tccactttac ggcttttcgc
     5641 catacctgcc aaaccacccg tgaggcggga aaccacgccg tctttatagg cgcgaagcat
     5701 atcgatgtcg agttccggtt cggggtattt gataccgttg gcagccaagt ggcgcacttc
     5761 gtcgataacg gcggcattgt gcaacaaggc tttggaaggg atacagccga cgttcaggca
     5821 aacgccgccc aaagttttgt aacgctcgac gatggcgact ttcaagcctt catcggcagc
     5881 ggcaaatgca gcggagtaac cgccgggacc gccgcccaat acgaccacgt cgtactcggc
     5941 atcggcagca ctgccgaatt gcgcggcttg cggagcagga gcggcagctt tgggtgcttc
     6001 ttgcgccggg gcggcagccg cttcggcttt aggggcggca gccgcacctt cggcttcgac
     6061 gacgacaatc aaaccgcctt cggagatttt gtcgccgact ttgactttca cttctttgat
     6121 cacgcccgca acttcggcag gcacgtccat cgtcgctttg tcggtttcca aggtaatcag
     6181 ggtgtcgtca acggcgatgg tgtcgccaac gttcacttca accgcgataa tatctacatt
     6241 ttcgtgtccg ccaatgtcgg gcactttcaa ttcaactaaa gccattatat tgacctttca
     6301 acttagttaa tttctatcaa ctctttagca ataatcactt cttcaggaat acagccacaa
     6361 tcttcagctc tgattgttac ttccttctca tctgggtttt ctggatgttc aacctcatat
     6421 tcaaaaatag aatattgacc aaacaaatct ctatttaaaa catatatata gccattttcg
     6481 atgccggagc ttgttgcaaa tttcttggca atttccttgt ctgttgtcgt agatatataa
     6541 catccgtcat atagatctgt ttcaatttga tgggcgtaaa ctgcattctt cacacttgga
     6601 ccatgtgtag ctttaccatc atatttaaac ttaccatcat aacgaattgc aacttcagct
     6661 ttattacctt taggttttaa ctgtccatta ttttgctcat cttgttggca actaatgcct
     6721 ctatataaat aagaaatttc ccataagtta ttaatttaaa gtttgaattt aacaccatca
     6781 ctcatcaatc tcaagcaatc caaacattat cagactgctt gagagggatg ttttatttta
     6841 taaggtaatg cggcggaagt ctttcaacag gttcgccagg aatacggtga agcgcatacc
     6901 ggcggcaccg tcgatgacgc ggtggtcgaa ggacaggctc aacgggcaca tcaggcgcgg
     6961 ggcaaactct ttgccgttcc aaaccggttt gatttgggat ttgcacacgc ccaagatagc
     7021 gacttcggga gcgttcacaa tcggcgtgaa acctgtgccg ccgatgccgc ccaagctgga
     7081 aatggtaaag cacgcgcctt gcatttcttg cggtttgagc ttgccttcgc gggctttttt
     7141 ggacaattcg gtcaattctt ggctgatttg tttcaagcct ttttgatcca cgtctttgat
     7201 gacgggaaca accaagccgt tcggcgtatc ggctgcgaaa ccgatgttga agtagttttt
     7261 cagcaccagg ttgtcgccgt ccaaagaagc gttgaattcg gggaaggctt tcagcgcgga
     7321 aacggaggct ttgatgatga acgccaacgg ggacagtttc acgccttcgc gttcccattc
     7381 tttgttcagc tgtttgcgga attcttccag ctcggtcata tccgcttctt cgtgtacggt
     7441 aacgtgcgga atcaccaccc agttgcggga caggttttga ccggagattt tcttaatgcg
     7501 ggacaattct ttaacttcga cattgccgaa tttggagaag tccactttag gccacggcag
     7561 caagtccaga ccgccgccca aagatgcgcc ggctgcggca ggttttgccg cgccgccctg
     7621 cattacggat ttcacaaagg ctttgatgtc gtctcccatg atacggcctt tcaagccgct
     7681 acctttgact tggcccaaat ccacgcccaa ttcgcgcgcc agtttgcgtg cggaaggacc
     7741 ggcgtgtgct ttggcgaaag cggcttcatc gattttggca gcggcaggcg aggcagatgt
     7801 aggcacggcg gcgggtgcgg cagcttgagc ggcaggcgca ggagctgctg cggcagagcc
     7861 ggcggtttcc acttcgataa tggcagagcc ttcggacact ttgtcgccga cttttaagaa
     7921 tacggcttta acgacaccgg cagcggtaca aggtacgtcc attgtcgctt tatcggtttc
     7981 caaagtaatc agcgtgtcgt cttcggcaac ggtgtcgcca actttgattt caacggcgat
     8041 tacatctaca tcggtatggc cgccgatatc gggaacggct acttgaacgg ttgcaccgcc
     8101 tgcggcagcg ggtgcaggtt gtgcttcggc ggcagcagcc ggggcggctt cggcagcggc
     8161 ggcaccggtt tcaacggtca aaattacgcc gccttcggag attttgtcgc cgactttgac
     8221 ttttacttct ttcacgacac cggccgcatc ggcaggcaca tccatcgtgg ctttgtcggt
     8281 ttccagtgta atcagggtgt cgtcaacggc gatggtgtcg cccgctttaa cttctacggc
     8341 gatgatgtcg acgctttcgt gaccgccgat atcggggact ttgatttcta cgatactcat
     8401 ttgagttcct ttaatctttt tcagttggat gccgtctgaa agctgcttca ggcggcatcg
     8461 gtacggtttg tctgttcaaa ccattcgggc attcggtttt cagacggcct gatgttggca
     8521 tgaaacaaac aggccgtctg aaacatcaat cagcgtttcc agctaggggc tgaatcggct
     8581 ttgatgccgt atttctcaat ggcttgttga acggtttctt tgctgacttt gccttgttcc
     8641 gccaatgcgg ccaatgctgc cacggcaacg ttgtagcggt ccacttcaaa gaagcggcgc
     8701 aggttggcgc ggctgtcgga acggccgaaa ccgtcagtgc ccaagacatg gtagtcgttc
     8761 gggatgtacg cgcggatacg gtcggcatag ctgcggatat agtcggtagc ggcgataacc
     8821 ggaccgtcat gaccttgcag ttgggaagta acgaaaggca ctttttcagc ttccagcgga
     8881 tgcaggcggt tgaagcgttc ggtttcgatg gcgtcgcggt gcagcaggtt gaaggacggg
     8941 caagaccaga tgtctgcttc cacgccgaag tcggctttca gcagctcggc accggcaatc
     9001 acttcttgca gaatcgtacc ggaacccatc aattggactt tcttgtcgcc tttgccgccg
     9061 gctttcagca ggtacatacc tttgaggatg tcttgttccg caccttccgg catatcggga
     9121 tgggtgtagt tctcgttcat cagggtgatg tagtagaaca cgtcttcatt attggcatac
     9181 atacggcgca gaccgtcttg tacgatgacg gccacttcgt attggaaggt cgggtcataa
     9241 gatacgcagt tcggaatcag gtcggcctgg atgtggctgt ggccgtcttc gtgttgcaga
     9301 ccttcgccgt tcagcgtcgt acggccggca gtaccgccca gcaggaagcc gcgcgcgtgc
     9361 atatcgcccg ccgcccaagc caagtcgccg atacgttgga aaccgaacat ggagtagtag
     9421 atgtagaacg gaatcatcgc gaagtcgctg ttggcgtagc tggtcgcagc cgcaatccag
     9481 tcggccatcg cgcccggttc gttaatacct tcttgcaaga tttgaccgtc gacggattct
     9541 ttgtagaaca tcagttggtc tttatcttga ggcgtgtatt gttggccttt gggattccaa
     9601 ataccgtatt ggcggaacat accttccatg ccgaaagtac ggctttcgtc gggaacgata
     9661 ggtacgacgc gtttgccgat ttttttgtct ttcagcaaag tggacaggat gcggacgaat
     9721 gccatcgtgg tcgagaactc gcgttcaccg ctggatttga gttgtgcgtc gaatgccgac
     9781 agctcgggca cttccaatac ttcctgcgtc ggtttgcgtt gcggcaggta gccgcccaaa
     9841 gcatcgcggc gtgcgtgcag gtatttgtat tcttccgtat cgggggcaaa agtcaggtaa
     9901 ggcagatcgc cgctttcgat ttgttcgtcg gtaaccggaa tgtcaaagcg gtcgcggaat
     9961 tgtttcaggg acgctttgtc catttttttg gcttggtggg caacgttctg accttcgccg
    10021 gatgcgccca taccgtaacc tttaatggtt ttcgccaaga taacggtagg tttgccgtcg
    10081 gcatggttcg ctgcgcggtc gtaggcgttg tacacttttt gaggatcgtg gccgccgcgg
    10141 ttcaatgccc agagttgctc atcggtcata tcggcaacca atgctttcag ttcgggcgta
    10201 ttgaagaagt gttcgcgcac atacgcgccg tctttggatt tgtaagtttg gtagtcgccg
    10261 tccaaacatt cttccatacg ttggcgcagg ataccgtctt tgtctttcgc caagaggcgg
    10321 tcccaacggc ggccccaaat gactttgacg acattccagc cggcaccggc aaagttgcct
    10381 tccaattctt ggatgatttt gccgttgccg cgcaccggac cgtccaagcg ttgcaggttg
    10441 cagttgatga cgaaaatcag gttgtccaag ccttcgcgtg cagccagcgc gattgcacct
    10501 tgggattcgg gttcgtccat ttcgccgtcg ccgcagaaac accatacttt acggcctttg
    10561 gtttttgcca agccgcgtga ttccaagtat ttcaggaaac gcgcctgata aatcgccatg
    10621 atggggccca agcccatgga tacggtcggg aactgccaga agtcgggcaa gaggtggggg
    10681 tgtggatagg aaggcagacc gtgtccgtcc acttcttggc ggaagttgtt cagctggtct
    10741 tcggtcagac ggccttcgac gaatgcgcgt gcatagatgc ccggggcaac gtgaccttgg
    10801 aagaagacca aatcgccttc ttcaccttca cctttggctt tccagaagtg gttgaaaccg
    10861 acttcgtaca tggtggcggc agattggaaa gatgcgatgt gcccacccag ttccaaatct
    10921 ttcttgccgg cgcgcaatac gatggcggcg gcgttccagc gtacgaatgc gcgaatgcgg
    10981 tgttcgatgt tttggtcgcc cggaatgcct ttttcgtttt caaccgaaac ggtattcaaa
    11041 tacggggtgg tcgtgccgtg gggcatacgt acgcctttgt cgcggcagta tttgagcagg
    11101 ttttccaaga ggtattgcgc gcgttcgccg ccttcatatt cgaggacgga gcttaacgcg
    11161 tccagccact cttgggtttc gatagggtca acatcgtgta attgggtgga catagtatct
    11221 atcctttatg ttgagtgttt aatcggaagg gagggtgtag ccaacccctt tattttcgtt
    11281 tgtgaaaaat aataagcaaa aacgaaaatt tctaaatcaa accgaaacaa aataatttaa
    11341 gtttaatgtt ttcaaaaaat tataaaagtc ttggaagcaa gggaaaactt gaaaaaaaaa
    11401 atgttcggtc tgctccgcac gaaatcctat cagcaatcgg gtctgctggc aaataaccat
    11461 gcttttaaaa gcaggtttta tactgaagtt ggcgtttggt aaggacaggt ctttttgatt
    11521 gcgaaaacat tctgtttaat tttggttata ttttttcgga attgtgggat atcgaatcgt
    11581 tatgtctctg tcggcaattt tcctattgca gaaaatctat acacaagctt tgtggacaac
    11641 cgtgttgata agccacggct tgtgttaaaa aagtcttacg caacaaatgt acgcttattt
    11701 tgattaaaaa ttaggcaaat ttaaatctat aaaaatcatg taattatgtt caatatgatg
    11761 tttctggtca tgtttttgcc gtctgttatg gggtttgtcc cgcgtttggg gcagggggtg
    11821 aattttggag gttggtgcaa aacctgcttt tttcgcgtta caatctgcac ccgatttact
    11881 tttgaatctg cctgtttgga aaaatacttt tatgaaagcc agccaattct ttatctctac
    11941 tttaaaagaa gcccctgccg aagccgcgtt tgccagccac aagctgatga ttcgcgccgg
    12001 tctgattaaa gccaacgcgt ccggtcttta tacttggatg ccgatggggc tgcgcgtgtt
    12061 acgcaaagtc gaaaacgtcg tgcgcgagga aatggcgcgc gcgggcagcg tggagctgct
    12121 gatgccggtg gtgcagcctg ccgaactgtg gcaggaatcc ggccgctggg agttttacgg
    12181 taaagaactg ctgcgcctga aagaccgcca cgaacgcgat ttctgcatgg gcccgacctg
    12241 cgaggaagtc atcgccgaca tcgtgcgcaa agaaatcaac agctacaaac aactgccgaa
    12301 aaatttttac cacatccaaa ccaaattccg cgacgaagtg cgcccgcgtt tcggcgtgat
    12361 gcgcgcgcgc gaatttgtga tgaaagatgc ttattccttc cacgccgact acgcctcgct
    12421 tcaggcgacc tatgatgcca tgtatgacgc ttactgccgc atctttaccc gtctgggttt
    12481 ggcgttccgc cccgtcgccg cagacaccgg cagcatcggc ggtacgggtt cgcacgagtt
    12541 tcaagtgttg gcggaaagcg gcgaagatgt gattgcatac agcgacactt ccgattacgc
    12601 cgccaatatc gagttggcac cgaccttgcc gcttaaaggt gaacgtgccg ccgctcaggc
    12661 tgtgttgacc aaagtacata caccaaacgt caaaaccatt gagtctttgg ttgaattcct
    12721 gaatattccg gttgaacaaa ccctcaaatc catcgtggtt gaaggcgaaa acgaaggcga
    12781 actcgtccta ctgctgttgc gtggcgacca tgagtttaac gacatcaagg cagaaaaact
    12841 ggcgggcgta aaatcgccac tgactatggc aagccctgcc gcgattgttg aacaattcgg
    12901 cgcaaacggc ggttcgctcg gccccgtcgg cttcacaggc aaagtctatg ccgatttcgc
    12961 taccgaaaaa ggcgcggact gggttatcgg cgcaaacgaa gacgactacc actataccgg
    13021 cttcaacttc ggccgcgatg ccgccgagcc tgaattcgtc gatttgcgta atgtggtcga
    13081 aggcgacgaa agccccgacg ggcaaggccg tctgaaactg gcgcgcggca tcgaagtcgg
    13141 acatgtcttc cagttgcgcg gcaaatatac ccaagctatg aacgtcagct tcctcgacaa
    13201 taacggcaaa tcgcaaatca tggaaatggg ctgctacggc atcggcatca cccgcgtcgt
    13261 tgccgccgcc atcgagcaga ataacgacga aaaaggcatc atttggacca aagcgatggc
    13321 gccgtttgaa gtcgtcatcg tgccgatgaa ctacaaaaaa tccgacactg tgcgtgaagc
    13381 cgccgacaga atctatgccg aattgctggc ggcaggcgcg gatgtgctgc tggacgaccg
    13441 cgacgaacgc gcaggcgtat tgctgaacga ttccgagctt ctcggtatcc cgcaccgcat
    13501 cgtcatcggc gaccgcgcct tgaaagaagg caatgtcgaa tacgccgaac gccgcggcaa
    13561 cgaagcgcag gcagttgcaa tcggagaaat tgttgcgcgt gtaacagctt cattaaatgc
    13621 gtaagtctgg tcaaatgccg tctgaaaata atttcagacg gcatttttat tttgggggaa
    13681 agtgaagtgg cttcagtatt tttagaagga gaagcagtcg aggtcggcaa tattttctgt
    13741 atcggcagga actatgccgc acatgtcgaa gagttaaaaa acgaaatacc ttcagaacct
    13801 gtggtgttta tgaagccgtc aggcagcatt ttgaacagcg gcggtacaat cttgctgccc
    13861 gagttcagta gggatgtgca gtttgagtgc gaactagtgt tgctgattgg ccgggattca
    13921 gacggcacgg gcgagggtga agatattttg ggatgcgtgg cggggtacgg cgtgggtttg
    13981 gatttgaccg cgcgggacat ccaatgccgt ctgaaggcgc aggggctgcc ctggctgaag
    14041 gcaaaagggt tcaggcattc tgcgtgcgtg tcggattttg cggcggcaga cagaatcggc
    14101 aatcaagata aggtgttgtt ttcattaaaa caaaacggcg tattgaaaca gcgcggggag
    14161 actggtttga tgatttaccc gattcgggaa attttacaca aactggcggc ggattacggt
    14221 ttggggaaag gggatttggt gtttaccgga acgccctccg gcgtgggcgc aatcggtgcg
    14281 ggggacaatt tggcattgga gctggacggc ttggtgtgcg cctctttcac gatagggtgt
    14341 taaccgcgcc gcttaacctt cacgcccgtt gaaacattcc gccgggccgc cctcgagaaa
    14401 accgtgcagg atggcttgcg ccgccacttg gtcgagcacc gatttgcgtt ttttgcccaa
    14461 gacctgtgct tccgaaagca ggctttcggc atagacggac gacagccgtt cgtcaaccca
    14521 atagacgggg agattgaacc tgccgttcag cctgcgtcca aacttgcgcg acaggtgcgt
    14581 catttcatgt tccgtgccgt cggcatgcac gggcagtccg acgacaaaat aacgcggctg
    14641 ccattcttga accagcttgg cgattgccgc gaatttttca tcgttgctgc cgccggtaac
    14701 ggtggccagc ggatgggaca gccccaattc cgcatcgccc tgcgccacgc cgatgcgcgc
    14761 ttcgccgaag tcgaatgcca gtgccgttcc ttttggaatt ttatgcatgg ccggctcccg
    14821 aaaacagggc gagcgggtcg atgccgagtt tggcgaatgc ggcggcgtaa cggtgttcgt
    14881 aggggatgtc gaacaggatg tgttcgtcgg cgggaacggt cagccacgca ttgtcggcaa
    14941 gttcgcgttc gagctgccct ttgctccagc tcgaatagcc tatgctgatc aaggctttgt
    15001 caaccgcgcc ttcgcgtgaa atgttttcaa gcacatcgcg ggaagaggtc agcgcgatgc
    15061 cgtctgaaac gccgatgctg ctttgccagt tgccgatcgg agtgtggacg acgtaaccgc
    15121 gctcgacctg caccggcccg cccatcatca cgctgtcgtg ctgcatccgc atggggatgt
    15181 ttttgccggt ggcggaaaaa atcatgtcca tcgtaatcgg agagggtttg ttgatggcga
    15241 taccgagtgc gccgtcttca tcgtgtttgc agatatagac gaccgattgt gaaaaaaacg
    15301 cgtcttccat atcgggcatg gcgacgagaa agtggttgga caggttcata tcagcgtttg
    15361 tcgggatttt ttatcgggtt ggaaataatg caggcgccgg caagcagttt gccggaagta
    15421 tcggcttgca ccccgtcggt ttgcggtatg ccgctgccga aacaggaaac cgcgcttaag
    15481 gaatttttcg gcgatccttg gacgatttta tcgctgtaaa ccaaataggc gaaggctttg
    15541 cgtttggggt cgtaataacg gacaatctgc cggctcttga acgcgaagcc cgtaccgcgc
    15601 ttgaaaactt ctttcggttt gcgcacggcg gtttcgtcaa aagaaatcga agatgccgtc
    15661 tgaacgcacg aaaccgatgc gtcggacgcg tcctcttcca aattgaccat ttccttcaag
    15721 ccgccttttt ttgcatacga aatataacag gcaacccctt gaacgtcggg atcgtcgaat
    15781 ccttccactt cgatacggtc gtttttgccc aacatgttga aaacggtact tgcccgtccg
    15841 attttatcgg tttcgccgct gccgcaggca gtcggcagga cggcggcaga cagcagtagc
    15901 agtctgttca tcaaaatttc cttatcggtt gaatccccgt tttccggaag gcggcagggt
    15961 ttgctttgtt tggaggggcg cacgctccca aatcgggcaa catacggtac gcagcttaca
    16021 tgtgctaccc tgtgtgtcgg aacacaatat ccgttgcagc attgaaaata tgccgcacaa
    16081 tataaggctg tttgagccga ttgcaagtat ccgcgcaaag aaggtgcaag at
//
LOCUS       DAXDXW010000060        15986 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXDXW010000060 DAXDXW010000000
VERSION     DAXDXW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15986
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.061"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(76..339)
                     /locus_tag="ACFB64_001556"
     CDS             complement(76..339)
                     /locus_tag="ACFB64_001556"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209890.1"
                     /translation="MSNPKYVKKETNPLAYFKRFVPPLSRFYLSGQPLRRLKNSGFRL
                     CSGLKPMAGILFFSGISFVKQMVLNRLMFYAVFFLFLFFGILF"
     gene            344..2728
                     /gene="ppsA"
                     /locus_tag="ACFB64_001557"
     CDS             344..2728
                     /gene="ppsA"
                     /locus_tag="ACFB64_001557"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC1209891.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLNERISAALAKLDVEDVSELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVTDADGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKKAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYNQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVEVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRYAINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEIIRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSVYEPHEENPMLGFRGAARYVAESFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVGEGIESVSLNPDTVIETWLYLANELNK"
     gene            2949..4208
                     /gene="rho"
                     /locus_tag="ACFB64_001558"
     CDS             2949..4208
                     /gene="rho"
                     /locus_tag="ACFB64_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC1209892.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDSINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKIGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPASGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            4824..6128
                     /locus_tag="ACFB64_001559"
     CDS             4824..6128
                     /locus_tag="ACFB64_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC1209893.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAARMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFIMA
                     AYSGLMSWAILKAVGKICGGLRVGKDVEREGLDLNIHGEHVE"
     gene            complement(6222..7517)
                     /locus_tag="ACFB64_001560"
     CDS             complement(6222..7517)
                     /locus_tag="ACFB64_001560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC1209894.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKAETCIIGGGLSG
                     LCTALPLAEHGHEAVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVASAAAEAGAQIFEQSPMTRIEP
                     YQNGWLVYTPEGSVECKNVVYAVNTYVGLNPIFRPLERKAIAVSTFIIATEPLGARTK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVRIEYSWGGECDITANLVPHFGRLTSNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFEQLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(7644..8531)
                     /locus_tag="ACFB64_001561"
     CDS             complement(7644..8531)
                     /locus_tag="ACFB64_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC1209895.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     NREAAEAYRQEKLAAEKAN"
     gene            complement(8531..9496)
                     /locus_tag="ACFB64_001562"
     CDS             complement(8531..9496)
                     /locus_tag="ACFB64_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC1209896.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISSA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIINNLLIKMGIIREPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDSRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(9512..10636)
                     /gene="potA"
                     /locus_tag="ACFB64_001563"
     CDS             complement(9512..10636)
                     /gene="potA"
                     /locus_tag="ACFB64_001563"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC1209897.1"
                     /translation="MTATTASSAKPYLQIQGLVKKFGDNYAVDNIDLNIYQNEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIDARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIENHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(10914..11183)
                     /gene="rpsO"
                     /locus_tag="ACFB64_001564"
     CDS             complement(10914..11183)
                     /gene="rpsO"
                     /locus_tag="ACFB64_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC1209898.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(11264..11398)
                     /locus_tag="ACFB64_001565"
     CDS             complement(11264..11398)
                     /locus_tag="ACFB64_001565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209899.1"
                     /translation="MWSDVKCRLKPEDVSVFRRHFADIAKYRAEQQKLCDNALFLFQE
                     "
     gene            complement(11388..12323)
                     /gene="secF"
                     /locus_tag="ACFB64_001566"
     CDS             complement(11388..12323)
                     /gene="secF"
                     /locus_tag="ACFB64_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC1209900.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVARGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKMGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVNNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHTVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIAKEAKQKEEIVV"
     gene            complement(12327..14180)
                     /gene="secD"
                     /locus_tag="ACFB64_001567"
     CDS             complement(12327..14180)
                     /gene="secD"
                     /locus_tag="ACFB64_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC1209901.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAENSLTVPLQDAGDVQKALPQLLKLFPEATLNSEGSNIVLALSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPIIQQSGLDRIVVQLPGVQDTAKAKDIIGRTAT
                     LEVRMVEDDPAKLREALEGNVPSGYELLSSGGEHPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(14392..14658)
                     /gene="yajC"
                     /locus_tag="ACFB64_001568"
     CDS             complement(14392..14658)
                     /gene="yajC"
                     /locus_tag="ACFB64_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC1209902.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKAGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            14789..15907
                     /locus_tag="ACFB64_001569"
     CDS             14789..15907
                     /locus_tag="ACFB64_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC1209903.1"
                     /translation="MPLTRLFLKLYALLRLFLGKNAQTAWIFHPACTGHDPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTVEAEEISDTRLALVRSSKYLNRLESCLPEDGKICRL
                     DDDTVISKNSLSAARFSAGSAVQAVDMVMKRKAWHAFCAARPPGHHAKSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NGMPDGGNMVHLPLPSGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASSCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLNPTKKRFAKPKTGQVRQPTQSDRYDINAV"
BASE COUNT         4025 a         4361 c         3999 g         3601 t
ORIGIN      
        1 aacatatctg ctaatctggt acagacccaa aaacaaatca tattttaaaa caaattatct
       61 caaaatcaga tttctttaaa ataaaattcc aaaaaaaaga aacaggaaaa atacggcata
      121 aaacattaac ctattcaaaa ccatctgttt tacaaaggaa atacccgaaa aaaacaatat
      181 gcctgccatc ggtttcaaac cgctacacag ccggaacccg ctatttttca gacggcgcag
      241 cggttgtccg gacagataaa aacgtgacag cggcggaacg aatcgcttaa aataagcaag
      301 cggatttgtt tcttttttaa catattttgg attggacaca caaatggccg acaactacgt
      361 aatctggttt gaaaacctgc gtatgacaga tgttgaacgc gtgggcggta aaaacgcctc
      421 gctgggcgaa atgatcagtc aactgaccga aaaaggcgtt cgcgtccccg gtggctttgc
      481 caccacagcc gatgcctacc gcgcatttct cgcacacaac ggtctgaacg aacgcatttc
      541 cgccgctctg gcaaaattgg atgtcgaaga cgtttccgaa ctggcacgcg tcggcaaaga
      601 aatccgccaa tggattttgg acacgccctt ccccgaacag ctcgatgccg aaatcgaagc
      661 ggcatggaac aaaatggtta ccgatgccga cggcgcggac atttccgttg ccgtacgctc
      721 ttccgcaacc gccgaagacc tgcccgacgc atcatttgcc ggccaacagg aaaccttcct
      781 gaatatcaat ggcttggata acgttaaaaa agcgatgcgc cacgttttcg cctccctgta
      841 caacgaccgc gccatttcct accgcgtcca caaaggcttc gaacacgaca tcgtcgccct
      901 ttccgccggc gttcaacgca tggtgcgttc cgacagcggc gcatcaggtg tgatgttcac
      961 cctcgacacc gaatccggct acaaccaagt cgtctttgtt acctcctctt acggtctggg
     1021 cgaaaacgtc gtacaaggcg cggtcaaccc ggacgaattt tatgtgttca aacccacgct
     1081 caaagcaggc aaacccgcca tcctgcgcaa gaccatgggt tcgaaacaca tcaaaatgat
     1141 tttcaccgac aaagccgaag ccggcaaatc ggtaaccaac gtcgaagtcc ccgaagaaga
     1201 ccgcaaccgc ttctccatta ccgacgaaga aatcaccgag ttggcacact acgcgctgac
     1261 catcgaaaaa cactacggcc gcccgatgga tatcgaatgg ggacgcgacg gcctggacgg
     1321 caaactctac atcctgcaag cccgccccga aaccgtcaaa tcccaagaag aaggcagccg
     1381 caacctgcgc cgctacgcca tcaacggcga aaaaaccgtc ttgtgcgaag gtcgtgccat
     1441 cggtcaaaaa gtcggtcagg gcaaggtgcg cctgattaaa gatgcttccg agatggattc
     1501 cgtcgaagcc ggcgacgtac tggttaccga catgaccgat ccggattggg aacccgtgat
     1561 gaaacgtgct tccgccatcg tgaccaaccg cggcggacgc acctgccacg ccgccatcat
     1621 cgcgcgcgaa ctgggtattc ctgccgtcgt cggctgcggc aatgcgaccg aactcttgga
     1681 aaacggtcag gaagttaccg tatcctgtgc cgaaggcgat accggcttta tctatgccgg
     1741 cctgctggac gtacaaatta ccgatgtcgc cttggacaat atgccaaaag cacccgtcaa
     1801 agtcatgatg aacgtcggca atcccgaact cgcattcagc ttcgccaatc tgcccagcga
     1861 aggcatcggc ttggcgcgta tggaatttat catcaaccgc caaatcggca tccacccgaa
     1921 agccttgttg gaatttgaca aacaagacga cgaactgaaa gcggaaatta tccgccgcat
     1981 cgccggttac gcatcccctg tcgacttcta cgtcgataaa atcgccgaag gcgtggcaac
     2041 gcttgccgca tcggtttatc cgcgtaaaac catcgtccgt atgtccgact tcaaatccaa
     2101 cgaatacgcc aacctggtcg gcggcagcgt atacgaaccg catgaagaaa acccgatgtt
     2161 gggcttccgt ggtgcggcgc gttatgttgc cgagagcttc aaagactgtt tcgccttgga
     2221 atgcaaagcc ttgaaacgcg ttcgtgacga aatgggtctg accaacgtcg aaatcatgat
     2281 tccgttcgtc cgcactttgg gcgaagccga agcagtggtt aaggcattga aagaaaacgg
     2341 cttggaacgc ggcaaaaacg gcctgcgcct gattatgatg tgcgagctgc cgagcaacgc
     2401 ggtattggcg gaacaattcc tgcaatactt cgacggcttc tccatcggct cgaacgacat
     2461 gacccagctg accctcggcc tcgaccgaga cagcggcttg gtgtccgaat cgtttgacga
     2521 acgcaaccct gccgtcaaag tgatgctgca tcttgccatc tccgcctgcc gcaagcagaa
     2581 caaatatgtc ggtatctgcg gtcaaggtcc gtccgaccat ccggactttg ccaaatggct
     2641 ggttggagaa ggcatcgaaa gcgtgtctct gaacccggat accgtcatcg aaacttggct
     2701 gtatttggcg aatgaattga acaaataatc aattcccata cccccaagcc tgaaaagcgc
     2761 gggggtattt tttccaagca gctccgttca gacggcattt cctgtcgatg ccccgtccgc
     2821 gataatattt gacacccacg cgccgactgc ctacaattcc cccctctccg agcaaccggc
     2881 aacagtcagc ttcttctttc agacggcatc cacccgtctt ttcctttcta ttctatatat
     2941 cattgattat gcacgtctcc gaattacaaa ccctgcacat ttccaaactc ttagaattgg
     3001 cggaagaaca cggcatcgaa aacgccaacc gattccgcaa acaagacctc gtatttgcca
     3061 tcgtccgcca gatgatgaaa aaaggcgagg gttttacctg ctccggcacg ctcgaaatcc
     3121 tgcccgacgg cttcggcttc ctccgcagcg cggacacgtc ctatcttgcc ggccccgacg
     3181 acatctatgt ctcgcccact caaatccgcc gcttcaacct gcatacgggc gacaccatcg
     3241 aaggcagcgt gcgcgtcccc aaagacaacg aacgctattt tgccttggtc agactggatt
     3301 ccatcaatgg cgaccacccg gaagtatgcc gccataaaat cctgtttgaa aacctgaccc
     3361 cgctgtttcc gaccgaacag ttgaagctgg aacgcgactt aaagtccgaa gaaaacctga
     3421 ccggacgcgc catcgacctg atttcgccca tcggcaaagg tcagcgcgcc ctcttggttg
     3481 ccccgcccaa aatcggtaaa accgtgatgc tgcaaaacat tgcccacgcc gttaccgcaa
     3541 actatcctga agtcgaactc atcgtcctct tgattgacga acgccccgaa gaagtaaccg
     3601 aaatgagccg ttccgtccga ggtgaagtag tctcctccac ctttgacgaa ccggcgcaac
     3661 gccacgtcca agttgccgaa atggtgcttg aaaaagccaa gcgtatggtg gaacacaaaa
     3721 aagacgtggt catcctgctg gattcgatta cccgccttgc ccgcgcctac aataccgtcg
     3781 tccccgcctc gggcaaaatc ctgaccggcg gtgtcgatgc caacgcgctg caccgcccca
     3841 aacgtttctt cggtgcggcg cgcaacgtgg aagaaggcgg ttcgctgacc attatcgcca
     3901 ccgcattggt tgaaaccggc agccgtatgg acgatgtgat ttacgaagaa ttcaaaggta
     3961 ccggcaatat ggaattgcac ctcgaccgcc gtatggcgga aaaacgcctc ttccccgcca
     4021 tcaacatcaa caaatccggc acgcgccgcg aagagctgct tgtgccgaac gaccagttgc
     4081 aacgtatgtg gttactgcgc aagttcctgc acccgatgga cgaaatcgaa gctaccgagt
     4141 tcttaaacgg aaaaatcaaa gcatctaaaa ataatgatga tttctttgaa ctgatgcgcg
     4201 gaaaataaac gcgccgcccg cattaatgcg aaatgccgtc tgaagcctga aaatcgggtt
     4261 tcagacgaca ttttcattcg cacggccggc gcagctcccc ccccccgcta acggcgcaac
     4321 cgccggcagt gtccgtgtcc gcctgccgaa ggcgcggcct ttgcaaaacc ggctcgaacg
     4381 catccctacc ggcaatgaaa accgatgcgg gaacttgatt caaggttgcg ccgaaccgcg
     4441 cccatttttg tataaatttt attttttatt tccaattaga aatttttaat atattaatca
     4501 ataaattaat tttataaaat aaaaatattg tcaacaatct tttgacttat cgcccaaacc
     4561 tctgtatatt ttcctacagt aaattgttga caatccatac gcccgcatat gcgccgccta
     4621 aggataaatc ctcccgccgg acaacggggg caagggatag gatgcgatat ttccatattc
     4681 aaacaaggga tttgtttcac gcacagggcg gcacatcggc aaaatccccg cgccggtccg
     4741 gcagggcttg cgcccatccc ggacaagccc cgaccccgcc tttccgaaag acgggcccaa
     4801 ccattaagga aactttaatc aaaatgaaaa aacacatatg ggcggcatct ttgctgccgg
     4861 catccctatc ggcagaacct ttaaactggt ggaaacctta ttccgccgtc aattcgggcg
     4921 ataccgcctg ggtgatgact gcggctgcct tggtactgtt gatgacgctt cccgggctgg
     4981 ctttattcta cggcggtatg gtgcggaaaa aaaacctgct ctcgacgatg atgcacagct
     5041 tttccatcgc gacattggtg ggcatccttt gggtcgccgt cggctattct ttggcgttca
     5101 cgccgggaaa tgcctttatc ggcggtttgg ggcgcgtatt tttaagcggg atgcagatag
     5161 acgctgccgc acggatgctg accgtgtcgc ccaatgcgcc gactgttccc gaaccggtat
     5221 ttatgttttt tcagatgacg tttgccatta tttcgactgc cattattacc ggcgcgtttg
     5281 ccgaacgaat gaaatattcg gcaatgatgc tgttttcggg catatggttt ttattggttt
     5341 atgtgccggg cgcgcattgg gtgtggggcg gcggctttat gagcaagggc ggcgtattgg
     5401 attatgccgg cggtacggtg gtgcacatca atgccggtat cgcgggactc gtcgccgcct
     5461 tggttttggg caggcgcata ggctacgggc gcgaggcgat gcctccgcac aatatggcga
     5521 tgacgctgat tggcgcggca atgttgtggt tcggctggtt cggctttaac gctggttcgg
     5581 cgcttgcggc agacgcggcg gcggggatgg cgatggcggt aacgcaggtg tcggccgtat
     5641 tcggcgcggc aggctggctt gcctgcgaga aaatagcggg acacaaacct tctgctttgg
     5701 ggctggcttc cggcgcagtt tccgggctgg tcggcatcac ccctgccgcc ggctttaccg
     5761 gtccgtcggg cgcggccgcc atcggtatat tgactgccgc cgcgtgcttt gtgtccgtca
     5821 ccgtcgtcaa acacaaattg cgttacgatg attctttgga cgctttcggc atacacggat
     5881 tcggcgggct ggtaggcgga atattgaccg gcatcttttt cgacaaccgc attttcggtg
     5941 gggatgcggc agtttggcag cagttgtgga tacaggtaaa agacgggttc attatggcgg
     6001 catacagcgg gctgatgagt tgggcgattt tgaaggctgt ggggaaaatc tgcggcggtc
     6061 tgcgcgtcgg caaggatgtc gaacgcgaag gtttggatct gaatatccac ggcgaacacg
     6121 tggaataagg acggctatgc cgtctgaagc ctgaaaatcg ggtttcagac ggcatttttc
     6181 acgtttgccg ccgatgggga taaacatact gcctgcgacg cttaacggct gtctttccac
     6241 tgataatatt tcgagccgag gaaagaaccg agtttgcgca aaaacggttg caggataata
     6301 ttcggctggc gcaactgttc aaacggtttc agacggcatt cgtcccctaa aattgcttcg
     6361 gcaaccgcca ggcctgcaat gcccgttatc gccatcccgt gcccggaata accttgcgta
     6421 taaaaaacat tcgaggtcaa acgtccgaaa tgcgggacaa ggttggcggt aatatcgcac
     6481 tccccgcccc acgaatattc gattctaaca tcggcaagct gcggaaaaac tttaagcata
     6541 tcttggcgga caagctcggt catacgcgca ggattgtcga taaattcgtt atccttaccg
     6601 ccgaaaagca gcctgccgtc cgcgctgagg cggtaataat ccaaaatatg gcggttgtcg
     6661 catactgcca tattgttgcg gataagccct tttgtgcgcg cgcccaaggg ttcggtggca
     6721 ataataaagg tgctgacggc aatcgccttg cgttccaaag gccggaatat cgggttcaaa
     6781 ccgacataag tattgacggc atagaccaca tttttacact cgacgctgcc ttcgggcgtg
     6841 taaaccagcc aaccgttttg atacggttcg atgcgcgtca tcggggattg ctcgaaaatc
     6901 tgcgcgccgg cttcggcagc ggcgctggca acacccaacg tgtaattgag cggatgaaga
     6961 tgcccggaca agggatcgaa ctgtgcgcct tggtacatat cgctgtcaag ctgctgtttc
     7021 aactcggctt tatcccaaag ttgataatga ctcgcaccgt aatgccgttg ggcgtgttca
     7081 tgccactgct gcaactcttc ccaatgctgc ggacggacgg caaccgtggc ataaccgcgc
     7141 tgccaatcgc aatcgatggc atgtttgcgg acgcgttcgt ccaccagttc gaccgcctgc
     7201 aaagactgtt gccaaaacca ttgcgcctgc tccaagccga cctgtttttc aatttccccc
     7261 ataccgcagg cgtagtcgct gataacctgc ccgccactcc tgccggacgc gccgaagccg
     7321 atacgtgcgg cttccaaaac gacggcttca tgtccgtgtt ccgccagcgg caatgcggta
     7381 cacaaaccgc tcaaaccgcc gccgataatg caggtttcgg ctttcagacg gcattggagt
     7441 ttcggataaa cagtatgcgg attaaccgaa ctaaaataat aagaaggcag atattcttga
     7501 aaatcagggc gaatcattgt gtttgcttta tcgggtatat tttcggacgg aatgatacag
     7561 gctgtcgggc catatcgtcc aaacagaaaa tcggttgaaa aaaaacaggc tgacccagtc
     7621 atgcggtcag cctgccttat taattaattt gctttctcgg cagccaattt ttcttggcgg
     7681 taggcttctg ccgcttctcg gttgcgcttg gttgcctgcc tcatcatcca ataattgacg
     7741 atgatgacca atgttccgat gatgccgatc aggatggttg ccaagacatt catctgagga
     7801 tcgagaccca acttgatctt ggagaaaata acctgcggca atgtggatga accggggccg
     7861 gagaggaatg aggtaatcac caaatcatcc aaagacaagg taatgccgag taggaagcct
     7921 gaagcaatgg caggggcaat caaaggcaaa gtgatgacaa aaaagatttt cagcggacgc
     7981 gcgcccaagt ccattgcggc ttcttcgagc gactggtcaa gctcgaccag ccgcgaacgg
     8041 atgacaacgg taatgtatgc catgcacagc gtcgtatgtc cgaggaagat ggtgaaaaag
     8101 ccgcgatcga agtagagatg ttgcaaccat tcgctgccct gcaaaaatat ctgcacctga
     8161 ataatcagca gcagcataga cagaccggta atcacgtcgg gcatgaccat gggcgcggaa
     8221 atcataccgg caaacagcgt gctgccgcga aagcgtttga tacgcgccat cgcatagccc
     8281 gccagcgtgc ccaaaacgac ggcggcaagc gaagacacaa cggcaatccg cagcgacagc
     8341 caagcggctt ccaaaatggt gtcgttttcc agcaatgcgc cgtaccactt ggtcgaaaag
     8401 ccgccccaaa cggtaacaag tttggattcg ttgaacgaat agatgaccaa aacaaccagc
     8461 gggatataca gaaacgccag cgatagtgcc aacatcagtt tcaagaacca agataatttg
     8521 gatttctgca ttatttggct ccttcttcca attcgcggtt ttcatagtgc tggaacaggg
     8581 caatcggtac aaccagtagc gcgaccatca cgacggcgac ggcagaagcc aacggccagt
     8641 tgttttgatc aaagaatgcc tgccacaata ctttaccgat catcaggttt tccgaaccgc
     8701 cgaccagctc gggaatgacg aactcaccga cagcggggac gaaaaccagc atcgaacctg
     8761 caataatgcc ggttttcgac aaaggcaggg taatcgtcaa gaacgatttg accggtcccg
     8821 cgcccaagtc ggaagccgct tcgagcaggc ggctgtccag tttcaccagc tgcgtgtaaa
     8881 gcggcagaat cataaacggc agataggcat aaaccattac caaatttaac gaaaacgcat
     8941 tatagaacaa atctaatggt tctctgataa tacccatctt aatcaataag ttgtttatga
     9001 tgccgttatg cccgagcaaa cccatccacg catagacacg cagcaggaac gatgtccaaa
     9061 aaggtaacat gatggcaagc agcaaaccat tgcggacgga aggattggca cgggaaatcg
     9121 cataggcggt cgggtaaccg accaacaggc aaatcaccgt cgtcgtcagc gcagtcttaa
     9181 ttgaagacca ataagtcatc agatagatat tactgttttc actgtcgccg aaggggttaa
     9241 gggtatgcca aaaattttgg aaaatatcgg catagttttg atagctgacg gcgatgttca
     9301 ggcgacccaa gtcctcatct atcgtcgtta aaggagtaaa cggtggaatg gcgatttctt
     9361 gttcggcaga gctgattttc agcacgatgg cgaacggaat cagaaacagc accaaaagcc
     9421 aaatatacgg tacggcaatc accgcacgct gccccggacg gcggaacagt ttgtttttca
     9481 gtttattaag gttcatctca ctccccttaa atcaacggaa caacggagtc ggttggtttt
     9541 ccggccagct gatatagacg gtttcatccc aagtcggcgg cgtgatgtta cgcacatacc
     9601 agtaaggtgc aggaacttgg cttttgacga cgcgcccgtt ggcgagtttg atatggtata
     9661 tggcgaagct gcccaaatag gcaatttctt ttaccgtgcc tttcgcccag ttgtagtcgc
     9721 ccaaatattc gggtttttct ttatataaat caatatcctc tggtcgaata ctaacccaaa
     9781 gatcctgctc gctcgggcca cccaaaccat ggtcgatacg gacgtggttt tccaaacctt
     9841 cgcattcgat aacggcatag tcggcatgat tttcaatcac cacaccgtca aagatgttgg
     9901 tttcgccgat aaactcggca gtgaagcggc tgttgggata gtcgtacacg tcgctgggcg
     9961 tgccgacctg ctgcaactga ccgtcggaca taatggcgat gcgggtcgcc atcgtcatcg
    10021 cctcttcttg gtcgtgcgta accataatgc aggttacgcc gacttgttcc agcgtattga
    10081 ccaactcgag ctgggtttgc tggcgcaatt tcttatccaa cgcccccaaa ggttcgtcaa
    10141 gcaacaggat tttcggacgt tttgccaaac tgcgcgccaa ggcgatacgt tgctgctgac
    10201 caccggacaa ttggtgcggt ttgcgtttgg caaatttggt catctgaacc agacgcagca
    10261 tttcttcgac gcgcgcatcg atttcgcctt taggcatttt gtcctgtttc agaccgaagg
    10321 caatgttttg ctccaccgtc atatgcggaa aaagcgcgta actttggaac atcatattga
    10381 tggggcggtc atagggtgca agtttggtaa tatcttgacc atcaaggatg atttttccct
    10441 gattgggact ttccataccc gccagcatac gcagcagcgt agattttccg ctgccggaac
    10501 tgcccaaaag ggcgaagatt tcgttttgat aaatgttcaa gtcgatgtta tcgacagcgt
    10561 aattgtcacc aaactttttc accaaacctt ggatttgcag ataaggtttg gctgaagacg
    10621 ctgtggttgc ggtcataatg gcaatactcc aataaaaaga cgagtaccgg caaaacggat
    10681 tttcgaatgg gtgataaaaa gctgtttgat tgctggcggg agtcgcaacg tctgatgccg
    10741 tctgaaactc ttgtgaagcg cacgggcagc atgaaataaa aaggggttct aaagaacccc
    10801 gcattatatt agtttataag gtttttaggg taataaatat gggtttgagc ttgtcggaaa
    10861 gcaacagaaa agaaaacacc gcccattttt ctgggcggtg tcggaaagcg caattattta
    10921 cgcagaccca agcgggtaat caacgcgcga tacgtatcgg gctgggtacg gcgcaaatag
    10981 gccaacaggc ggcggcgttg gctgaccatt ttcaacaggc cgcgacggct gtggtggtct
    11041 ttggggttgg ctttgaagtg gggggtcaga tcgttgatgc ggaaagtcaa cagggcaact
    11101 tgtacttcag aagagccggt gtcgccttct ttacgttgga aatctttaac gatttgtgct
    11161 ttttgttcta cggtcagtgc cataatggaa aaactccgaa aaataaaaag aaccccaaat
    11221 gggattcgga caagtttgcc aagcctcgac aacggcaaac tccctattcc tgaaacagga
    11281 acagcgcatt atcacacaat ttttgctgtt ctgcacgata ttttgcgatg tcggcgaaat
    11341 gccgtctgaa aacggagaca tcttccggtt tcagacggca tttgacatca gaccacaatt
    11401 tcttccttct gtttcgcttc cttggcgata ttgtcgcggc tcaaaccgaa catcagcaag
    11461 agcgggctgg caaccaatac ggaagagtag atgccgaaca cgataccgat ggtcagtgcc
    11521 atagaaaagc cgtgcaaggc tgcaccgccg aacaccagca tagaaacaac catcgcctcg
    11581 gtcgaaccgt gggtaatgat ggtgcggctc atcgttgcgg taatcgcgtt gtcaatgact
    11641 tccggcacgg tatgtccgcg catcgccggc ttgcggaaat tttcacggat acggtcgaag
    11701 acgacgacgg attcgttcac agaatagccc aatacggcaa ggatacctgc caagacggtc
    11761 agcgaaaatt cccattggaa gaaggcaaag cagccgagaa taatcacgat gtcgtgcata
    11821 ttggcgataa tggcagatac ggcaaaacgc cattcgaaac gcatcgacag gtaaataatg
    11881 atgccgataa cgacaaaacc taaagccatc aatccattat ttaccaattc ctcaccgact
    11941 tgcgggccga taaattcgac ttggcgcaaa gtaacgtcgg gactgtcttt tttcagcaaa
    12001 tccataacct gattggacaa ctgtgcggaa gtaacacctt ctttgttcgg caggcggatc
    12061 atgatgtgtt tgttcgtacc caatgcctga acctgtacat cacccatttt cagcgtatcg
    12121 aggcgttcgc gcatcttatt gacatccgca ccctgctgat attggacttc cattaccgta
    12181 ccgccggtaa attcgacaga gaaattcagg cctctggcaa ccaaaaagaa tacggcggca
    12241 ataaaggtaa ccaacgaaat gaaggttgtc agtttgccgt agctcataaa cggaatatcg
    12301 cgtttgattt taaagagttc catagcttac tccttacctg ccgcagtttc ggcttcaggt
    12361 ttccataccg caccgatgga aatattctgc aatttgcggc ggcggccgta ccacagattg
    12421 accaacgcac ggaatacgac gacggacgaa tacatcgaag tcagaatacc caagcagtgt
    12481 acgacggcaa aacctcttac cggtccggaa ccgaatacca aaagcgcgat accggcaatc
    12541 agcgaagtca ggttcgaatc aacaatggtc gcccatgcgt gttggaaacc gagattgatt
    12601 gcctgctgcg gcggcacgcc ggcacgcaat tcctctcgga tacgttcgtt aatcaagacg
    12661 ttggagtcga ttgccatacc caaagtcaac gccagcgcgg ccatacccgg taacgtcaac
    12721 gttgcctgca tggcagacaa aataccgatt aggaacagta tgttggcact caatgcaata
    12781 gtagaaaaga aacccatcag gcggtaataa accaccatga atgcagcaac gatggcaaaa
    12841 ccccataaag tcgaatggaa gcctttttcg atgttctcct tacccaaaga cggaccgatg
    12901 gtacgttctt cgacaatctg catcggtgcg gcaagagaac cggcacgcaa cagcaaagac
    12961 gtatcgttgg cttcggctgt cgtcatgctt ccggaaattt ccacgcgtcc gccggtaatg
    13021 gcagtacgga taaccggtgc ggttacaacc tcggattttc cttggtcgat caaaaccatc
    13081 gccatgcgtt tgccgacatt tgcggcagtc agttcgccga aaatgctgcc gcccgcgctg
    13141 tccaagctca gactgacggc aggtgcgccc atttggtcga aactcggttg cgcatcgttg
    13201 atgttgtcgc cggtcagctc gacctgtttg ctgatcagca gagtttcggg atgctccccg
    13261 ccgcttgaaa gcagctcata accgctcggc acgttgcctt ccaatgcctc gcgcaacttg
    13321 gcaggatcgt cctccaccat gcggacttcc aaagtcgcgg tacggccgat gatgtctttt
    13381 gccttggcag tatcttgcac gccgggaagc tgtacgacaa tgcggtccaa acctgactgt
    13441 tggataatcg gctctgccac acccaattcg tttacacggt tatgcagtgt agtgatgttt
    13501 tgcttcaccg catctgatcg tactttattc accgcctctt ccgaaagcgc caatacaata
    13561 ttgctgcctt ctgaatttaa cgttgcctca ggaaacagct tgagtaactg aggcagtgcc
    13621 ttttgcacat cgccggcatc ctgcaaaggg acggtcaggc tgttttcagc ctgacgcacc
    13681 gtgccgctgc ggattttttc acggcgcagt tcgcggcgga tgtcgcccga ataacgctca
    13741 aacgttttct gcatcgcggc tttcatatcg acctgcatgg tgaaatgcac gccgccgcgc
    13801 aggtccaaac ccaaaaacat cggattggct ttgattttcg ccatccattc ggggctgtcc
    13861 gccaacaggt tgagcgcggt aatataccct tcgcccaaag tgttttcgat aacgtcgcgc
    13921 gctttaagct gcgtttctgt gtctttgaaa cgcactttca gtgaattatc cacaacaaac
    13981 atcccgtcgg tctgaatgcc cgcgtttttc agcgcagcat ccactttgga ttgagtctgt
    14041 tcgttgatga tgatggcttg tcggttggtc gatacctgca cggcgggcgt ttcgccgaat
    14101 aggttgggca gcgaatacac tgcggcaacc gcaatcgtga acacaatcag cagatatttc
    14161 cataaaggat aacggttcat cattgttcct taatggttgg aaccccaccc tttcggtggt
    14221 gtcggaatcg ggctatttca gaagaggcaa aaacccctcc ctgccaggca agaccgaaag
    14281 gcagcatcct gaatatgccg ccctgcgtgt cggaacatgg tcaagccttc ggttggaatt
    14341 caaaacaaag tgccgcattc gggctttccc gatgcggctt gtcggcacaa atcaatcgac
    14401 ttttgcggca atcgcattgc gttccacttc gacctcgatt tttgtaccct gtccgatatc
    14461 cacggtaaaa aactgttcgc cgactctggt taccttaccc ttgaaacctg ccgccaagac
    14521 cactttgtcg ccggctttca aggcggcaag cattgcctga tgcgctttga atttcttttg
    14581 ttgggggcgc atgatcagga agtagaacac caccataatc aacaccaaag gagcaaattg
    14641 tgcaacagct tgattcataa tttatccgtt ctttctaaat atgattgaaa atcgagcggg
    14701 gtatataata gcataagacc gtaaacaata tatcgggttt gccgcccata cccaaccgta
    14761 tgccgtctgc accccctttc ccgaacccat gccgctgacc cgcctgttcc tgaaactcta
    14821 cgccctgttg cgcctttttt tgggcaaaaa cgcccagacc gcctggattt tccaccccgc
    14881 ctgcaccggg cacgaccccg gggcaaacca tcccgattcg cccgaccgca tcctctgcat
    14941 cgagcaggca ttgcgccgcg ccggtatttg gcagcacctc caaaccgtag aggcggaaga
    15001 aatcagcgat acgcgccttg cacttgtccg ctcgagcaaa tatctgaacc gtttggaatc
    15061 ttgcctaccc gaagacggca agatttgccg tttggacgac gacaccgtta tcagcaaaaa
    15121 ctccctgtcc gccgcccgtt tttccgccgg ttcggcagtt caggcagtcg atatggtcat
    15181 gaaacgcaaa gcgtggcacg ccttttgcgc cgcccgcccg ccggggcatc acgcaaaaag
    15241 cggcaaagcc ggcggattct gcctgctgaa caacgttgcc gccggcgtca tgcatgccat
    15301 tgccgaatac cgcctgaaac gcattgccgt catcgatttc gatgtccact acggcgacgg
    15361 tacggcagaa atattcaaag acgatccgcg catcctgttt ttcaacctgt ttgaaaccga
    15421 ccttttcccc ttccccgaaa acaacggtat gcccgacggc ggcaatatgg tgcacctgcc
    15481 cttgccgtcg ggaacgggca gccgcacatt ccgcgaagcc gtccgcagac agtggctgcc
    15541 ccggcttgcc gcattcaaac ccgaactggt gctgctgtcg gcaggattcg acgcacaccg
    15601 tctggacgaa tcgggcaggc tcaacctgca cgaggcggat tttgcctggc tgacacacaa
    15661 aatcattcag acggcatcca gctgccccgg caaaatcgta tccgtactcg aaggcggcta
    15721 cacccttgaa cctctggcac aatctgctgc cgagcatatc cgcgtactgg cggggctggg
    15781 caaatccgat gccgcaaccg cctatcagaa aacactgaac ccaacaaaaa aacggtttgc
    15841 caaaccgaaa accggacagg tgcgccaacc tacccaatcc gaccgttacg atataaatgc
    15901 cgtctgaagc cgattcggtt ttcagacggc atcattattt gacctgacgc aaacagaaag
    15961 ccttccgcgt gcttaaacac atctct
//
LOCUS       DAXDXW010000061        15349 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXDXW010000061 DAXDXW010000000
VERSION     DAXDXW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15349
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.062"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..237)
                     /locus_tag="ACFB64_001570"
     CDS             complement(<1..237)
                     /locus_tag="ACFB64_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFC1209904.1"
                     /translation="MKELKWIEEARKYLGAHEKVNGKSNPVLLAMLQEMGNFNQEQKA
                     WWKETDTPWCGLFVGYCLGKAGRAVIRDWYRAKAW"
     gene            complement(279..404)
                     /locus_tag="ACFB64_001571"
     CDS             complement(279..404)
                     /locus_tag="ACFB64_001571"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209905.1"
                     /translation="MPPETDFAKRRRVTGVEMETERVKPDVSVLRKPAIKGLAGV"
     gene            444..680
                     /locus_tag="ACFB64_001572"
     CDS             444..680
                     /locus_tag="ACFB64_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFC1209906.1"
                     /translation="MLRLQKWGNSAAVRLPARLLTALNAKIGDALETEIRNGELLIRP
                     VKRYRLSDLLAETEAAPPRAEGWEEMPDVGQEGV"
     gene            680..1027
                     /locus_tag="ACFB64_001573"
     CDS             680..1027
                     /locus_tag="ACFB64_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC1209907.1"
                     /translation="MYIPERGDIFHLAFDPAAGTEMKGGHYAIALSPRAYNRATGLVY
                     ACPISQGRAAAARSGGMISTLLGAGTATQGNVHCHRMKALDWKIRRAAFRETVPDYVI
                     EDVLARIGAVLFD"
     gene            complement(1082..1222)
                     /locus_tag="ACFB64_001574"
     CDS             complement(1082..1222)
                     /locus_tag="ACFB64_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209908.1"
                     /translation="MKFSINREFRKMLKLFAEYAVLRRMFYAVLGVAVYWRPPEILSA
                     LK"
     gene            complement(1383..1715)
                     /locus_tag="ACFB64_001575"
     CDS             complement(1383..1715)
                     /locus_tag="ACFB64_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209909.1"
                     /translation="MGFLQFGLLFAAAGGVLGAVWASLQERGRPVQAVLEAFISAIAA
                     AAAAERFVPLDQAWTCAAAGVFAGMMTGHALDTVRALAPKVLRGYLGGLAEKLTGVKD
                     GGSSDGKD"
     gene            complement(1716..2186)
                     /locus_tag="ACFB64_001576"
     CDS             complement(1716..2186)
                     /locus_tag="ACFB64_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209910.1"
                     /translation="MLRVLLNRRFLPAGFQAWLFGTATRVLEAVSGLGLSGYAAVFAL
                     APDEIYAWRIYYKFQDIPEAWTVGVLAAAGLLQTALLFARGVRACVASAYLLLFSGFV
                     WFLVSVAFWGAYPPLNTGMVVPPLLAFFCALAGNNALRFLFSAQKSRGLADEGS"
     gene            complement(2257..2562)
                     /locus_tag="ACFB64_001577"
     CDS             complement(2257..2562)
                     /locus_tag="ACFB64_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209911.1"
                     /translation="MIEDKKIVALNFAVEDGMTGASAAYHVVEHIGADYRNGYVTATL
                     NGYVSENAFKSGKRYLLARTLDFQETDITAPDPGWVYRKALEGAAGIPKDAQPVYAE"
     gene            complement(2674..6819)
                     /locus_tag="ACFB64_001578"
     CDS             complement(2674..6819)
                     /locus_tag="ACFB64_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFC1209912.1"
                     /translation="MGGKSSTVTSAEERILSLQVQRSSQGLTLPVIYGRTRVAGNLIW
                     YGDFVTIEHKTTTRRGGKGGGGVKQVGISYTYEAAVMLALCEGEIQGVGRVWRDKEKF
                     DSPAQLRLTLMRGGDEQPLWTHLQQAKHQGQALNYSGTAYLCSPNYELTKSAQIYQHN
                     FEVIGKSGYSGNIPDANPREIVLDLLTNQRYGCGFPSQNIGDTDRYSNYCRAVGIFLS
                     PAYTEQGEAQRNISELLEQTNSAAVFSQGRLKIIPYGDGSHSGNGAVYVADNKAAYDL
                     TDDDFIVSGAQDPVKAGRKTNADAFNQIQVEYLDRDNDYNVAIAEAKDQANIEQYGLR
                     PKDAVRMHGICDAKVAQKVAQQLLQRALYVRNEYEFKLGWKYCLLEPMDIVTLTDAGL
                     GLNKTPVRITEIEEDGEGVLSVKAEDCPAGVYTVSEYPTQPSSGYSADYNVSPGNAHV
                     PVIFEAPLQLTGGEPQIWLATAGGGMWGGAEVWVSADGDSYTRVGAVNRKARFGALTA
                     DLPDGAVFDRTNTLGVEISAGQLTGGTEQDSRDLLTLCYVDGEFLAYADAELKGVGRY
                     TLGNLTRGAYGSAVNAHAAGSRFARIDEALFKYAVPRNWIGRTVWVKLVSYNVFGGGI
                     QDLAEVPAYSYTIEGAPLGQIQNLRLTSSWAYGKEAVIAWDKLDGADTYDVEIYAGGS
                     RRRLRAVDGIVDNSYTYTQADMKADGGQVRGIVFKVRGRAVTGKTGNWAQIAAQNPQL
                     QALQGISIDSGLKQAFFTCQKPDEEDFAGIIVWVCENAACPAADANKAYDGAETFITI
                     AKCGGKPLEKGKTYYLRAAGYDSFGKDNLKISGSISFTVYDVSATDLSESSLNKALRD
                     KINLIDGNGAGSVNERVEAVRSTADGNAAAVQTHARSINGLEAQYTVKVDANGKVAGF
                     GLATTPKNGTPESKFIVNADRFGIGAAGKADVFPFVVDTQKNRVGINGELVVNGKAVV
                     DKLNAGDIHGGKIAADTLDANRLKAGSVTAREIGAAAVTADKIGANAVTADKIQVADL
                     SAVSSNLGSITGGSLNIGGGNFTVSSDGILTADNAVIRGRIEADSGYFNGTVRASSVE
                     GDVLRAHRLRWTEGNVWVLDLDKDPLPRVLIPNFYVVSETFGNNRVQAKLLLNGGVFA
                     PREVRETENYTNYIWRGRTFGRYEDLPPRARGGGDYTQQSGSRYRTRLEYQIQVIPAG
                     KPVSLKLSLASHESVFSPFVSVSYLAQSDYEYKQLLGRMVWRTFAESFRYDSKKQVYL
                     GGDRRVHDYQNQMYKHWNAYGGLLQLPDDIYGISFEYRPFTNADWSTMLAFDRSDKFV
                     VVKKYRGHAPQQYSLFQEEFNTAVPKSNLLFFVEHWWQYIELRNIRVLIPESRENEVW
                     QVG"
     gene            7059..7337
                     /locus_tag="ACFB64_001579"
     CDS             7059..7337
                     /locus_tag="ACFB64_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC1209913.1"
                     /translation="MESQTFASVFDALCDTPAQAANMRLRAGLMMHIADTVRENGWTQ
                     KQAAEHCGLTRPRINDLLNGKIDKFSLDALVNINAGLGQCISLSFAPA"
     gene            complement(7365..7796)
                     /locus_tag="ACFB64_001580"
     CDS             complement(7365..7796)
                     /locus_tag="ACFB64_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFC1209914.1"
                     /translation="MPSETDLRARIVEEARSWLGTPYHHHAMVKGAGVDCAMLLVAVY
                     GAVGLLPEGFDPRPYPQDWHLHRDCERYLGFVTQFCRETESPQAGDIAVWRFGRSFSH
                     GGILAGGGKVIHSYIGRGVVSDDIGQAELIGRGVRFFTFSF"
     gene            complement(7798..8652)
                     /locus_tag="ACFB64_001581"
     CDS             complement(7798..8652)
                     /locus_tag="ACFB64_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HFC1209915.1"
                     /translation="MKAATKELIDLLHGGDEFQMADLYTITLSGGRVLRHTGADMPVV
                     WDGQAYGAHELVIKRGATRTAVGLEVDSNTLQISAAPDYRLEGLQWAEAALGGVLDGA
                     RVRIDRVFFDAGLRPVGAVNIFSGRVSDVSGGRSSVKVDVKSDIELLNVSSPRNIYQA
                     GCMRTLYDDGCKVNREKFTVNGRVTENSRTGTVLKHNLTQPDGWFSQGVIKFAGGRNA
                     GLSRTVKAHGGNTFELALRLPYPPQAGDAFKVYPGCDKRRDTCKDKFDNIVHFRGFPF
                     IPSADTVV"
     gene            complement(8649..9248)
                     /locus_tag="ACFB64_001582"
     CDS             complement(8649..9248)
                     /locus_tag="ACFB64_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HFC1209916.1"
                     /translation="MGNAVFPEFPGLKWGRKKTAVWSTGTQKSASGREFRTAYYTYPQ
                     WRFSLSFEVLRTKASVNELEKLAGFFNARKGSFESFLYEDPADNAVTDQPVGNTVQGV
                     ARYQLVRSMGGFIEPVSAVKERPAVKVGGTALAYGRDYTVTDKGVLVFNTPQPPGRPI
                     TWTGGFYFRVRFTSDTVDFENVLGSLWAAKKIEFTSVKL"
     gene            complement(9248..9514)
                     /locus_tag="ACFB64_001583"
     CDS             complement(9248..9514)
                     /locus_tag="ACFB64_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209917.1"
                     /translation="MIAHVCASTGWTWDYVADNLDLPRIKHLNEYWREHPPVHILAAS
                     YMGVKPSSGSVQSEADEAEAVGMLGGGELSEDEFDALLKAKGII"
     gene            complement(9526..9855)
                     /locus_tag="ACFB64_001584"
     CDS             complement(9526..9855)
                     /locus_tag="ACFB64_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209918.1"
                     /translation="MTVRIKGVTVELNGADYVIPPIALGALEQLQERIGSFDGNAADA
                     GQISTVIDCAHAALKRNYPDLTREEAADLIDIGNMNEVFAAVMDVSGLKRREQEAAQA
                     GEARAAD"
     gene            complement(9916..10689)
                     /locus_tag="ACFB64_001585"
     CDS             complement(9916..10689)
                     /locus_tag="ACFB64_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209919.1"
                     /translation="MQLTFGSGEVFAQMITDAYGNRVQNATPVRIMGLQEMSVDLSAE
                     LKEFYGQNRYPLAVAQGKVKVSGKMKGALINGLTLNTLFFGTEYATGTMKALWAETTG
                     KVLDGDNYSYLQAAAPGGGKFAEDAGVMGQDGTAYIKVASSPKQGQYTVSESGVYAFN
                     SSDKGKTVYPSFTYTQTMPPAKKIELTNMATGNTPTFKMRYLTQFKGKKALLELESVT
                     SGKLGLFSTKNDDFSVPEIDFTASTDEAGFKIGTLWIQE"
     gene            complement(10715..11143)
                     /locus_tag="ACFB64_001586"
     CDS             complement(10715..11143)
                     /locus_tag="ACFB64_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209920.1"
                     /translation="MNREAVYSALWAKLDALDGFVTKSRKLVHWNDVKRYDQPALFMA
                     QGDMQALTLTGRETKWILRADVYLYVQTAGQPPAPVMNPLIDAVCNAVNAVHPVTGKT
                     DLTADGADIEYCRVEGTVETDEGTLGEQAVCIIPIVICAA"
     gene            complement(11140..11622)
                     /locus_tag="ACFB64_001587"
     CDS             complement(11140..11622)
                     /locus_tag="ACFB64_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFC1209921.1"
                     /translation="MLKMEFIGGGILAAVFKAYAADVQDAVVKSVGRSALRLQSEVML
                     NRLSGRVLRVRTGNLRRSVHQRVNVSGNVVSGEVDTNVRYGIAHEYGFAGSVNVKASL
                     RQVRQAFGKPLKPPRYVRVRAHTRDVKLPERSFLRSALRDLTPKFADDLQKSIGKVLK
                     "
     gene            complement(11622..12152)
                     /locus_tag="ACFB64_001588"
     CDS             complement(11622..12152)
                     /locus_tag="ACFB64_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFC1209922.1"
                     /translation="MAAPVSLEEFKQRIGVEHDRRDDFFLSVIDGVSAAAEAYIGRSL
                     LAADYVGRYDGNGKDRIVLDNYPVLSVSSVKINGADAGGWEFDNWLLMRPEGFARGLK
                     NVEVSYRAGYERMPADIREAVMIIAVQRVNEIEGKGVRSKTLAGETVAFSTFGNSGGM
                     PPSAFAILNEYKRKGV"
     gene            complement(12155..12517)
                     /locus_tag="ACFB64_001589"
     CDS             complement(12155..12517)
                     /locus_tag="ACFB64_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209923.1"
                     /translation="MPSENQTAFSFGDFEMTVKLKAPEGFTDVSFGSQSYAADENGIV
                     EVPSEAAEFLYQFGFGNVASEPAEGPEKAKRGRKPKTGQPAGQSEPAEAAEPAEAEAE
                     AAEPAEAEAEPAEAEKAE"
     gene            complement(12524..14023)
                     /locus_tag="ACFB64_001590"
     CDS             complement(12524..14023)
                     /locus_tag="ACFB64_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209924.1"
                     /translation="MNVNQLTQETIELMKSAQAGGGPPDKGFTQPAGFTAGLQTYDLS
                     APSQKLYPVLTPLRNRIPRVGGGRAIGSNWKAVTNINVGNQRAGIGEGRRGGVINHET
                     VERNAQFRAIGLENQVTFEADYAARGFEDVKALAVAQTLQATMVAEEMILLGGNTSLK
                     AGVTPTPTAVVSADAAGKLGVSTLSVICVALGLQAYWDVAGANNGATGQSPNIKTAQV
                     PAKITRRNADGTTDTFGGGSARKSAAASVSGIEAGKKVTAVIPAVRGAVAYAWYWGAA
                     GSEKLGAVTTAAKVEISADAEGTQTAASLPSEDNSTSILEFDGLLTQIALPDSGAFWS
                     DNKGGGLTSDNAGGVYEFEEAFAHFFTRYRLSPDTVYVNARDLAALTKLIIGNSGAPL
                     IKLNVDVNNTANIRAGVVVGSYMNKITGDDLNIVVHPNLPAGTYLFYSSRLPAYVQGV
                     GNLLQVRTRQEYYQIEWPLRTRMYEYGVYADEVLQGMFMPAFGMITNVG"
     gene            complement(14064..15221)
                     /locus_tag="ACFB64_001591"
     CDS             complement(14064..15221)
                     /locus_tag="ACFB64_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFC1209925.1"
                     /translation="MTKLYAQIAKTEAQDDGTVKVWGYASSEAVDSDGEVVAAEAMKA
                     AIPDYMKFGAVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVTKVKTGVYKGFS
                     IGGRVTARDDLNKSQITGLKLTEISLVDRPANPDAVFTCFKADKPKDEAGAADKDGKP
                     SDKPTEEEDENPKDGDKGPKTEDKGDKDAGKKDEAGKSASVNLSESEIAALKAVLAKA
                     DKPKGGTAAKSMYQVKSPADVLMSLKWLVEDASYDNIDEAVTAQIKESAAGLAESLKA
                     LAASEADKPADGLAAKAGKSGDLAKAESADELAKAQDALKKSNDALAKAQAEIESLKK
                     QAVPPKGSTKAISKAEDNGEDPLKGFQPIVKNDGTLDDVATLIKAKQTGRL"
BASE COUNT         3213 a         4860 c         3935 g         3341 t
ORIGIN      
        1 ccaggctttg gcgcgatacc agtccctgat gaccgcgcgt cccgctttgc ccaggcagta
       61 tccgacaaac aggccgcacc acggcgtatc tgtttctttc caccacgctt tctgctcctg
      121 attaaaattt cccatttctt gcagcatcgc taaaagcaca gggtttgact tgccattcac
      181 tttttcgtga gcgccaaggt atttacgggc ttcttcaatc catttcaatt ctttcatttc
      241 ttttccttcc gataaaagac gagatgtaaa ctttcggttt acaccccggc caagcctttg
      301 attgcaggtt tccgcaacac cgatacatcc ggtttcacac gttccgtttc catctccact
      361 cctgttacac gccgccgctt ggcaaaatcc gtttcaggcg gcataatgta tcgacaacgt
      421 atctacaaca cgaaagcaaa accatgttgc gactgcaaaa atggggcaac agcgcggcag
      481 tccgtctgcc cgcccgcctc ctgaccgccc tcaacgccaa aatcggcgac gcgctggaaa
      541 cagaaatccg caacggcgag ctgctcatcc gccccgtcaa acgctaccgc ctgtccgacc
      601 ttttggcgga aacggaagcc gccccgccgc gcgcggaagg ctgggaagaa atgcccgatg
      661 tcggacagga gggcgtttga tgtatattcc cgagcgaggc gacattttcc atcttgcctt
      721 cgaccccgcc gccggtacgg aaatgaaagg cggccattac gccatagccc tgtcgcccag
      781 agcttacaac cgcgcaaccg gcttggttta cgcctgcccg atttcacaag gcagggcggc
      841 ggcggcaaga agcggcggta tgatttccac actgctcggc gcgggaacgg caacacaagg
      901 caatgtccac tgccaccgga tgaaggcatt ggattggaaa atccgccgcg ccgccttccg
      961 cgaaaccgtc cccgattacg tgattgaaga cgtattggca cgcatcggcg cagtactgtt
     1021 cgactgactt cagagtgcga agcctgtccg cagggggaag catccgtttc catatccgcc
     1081 cctatttcaa agccgacagg atttcaggcg gccgccaata aaccgccacg cccaaaaccg
     1141 cataaaacat acggcgcaac accgcatact cggcaaacag cttcaacatc ttgcgaaact
     1201 ccctgtttat gctaaacttc aaacagattt ctccttacct cggataaggt taaattcaga
     1261 aacgccgcag ggattccgcc cctcgcggcg tttcgccgtt ttcaaacctg ccgcgtaatg
     1321 tcgtgcgtca gccggacgcg cccggaacac agcgtcttca cgcgccgcga taccgctcga
     1381 aatcaatctt ttccatccga agaccctccg tctttcacac ccgtaagctt ttccgccaag
     1441 ccgcccagat aaccgcgcaa aaccttgggc gcgagcgcgc gcaccgtatc gagcgcgtgc
     1501 cccgtcatca tccccgcgaa cacccccgcc gccgcgcacg tccacgcctg atccagcggc
     1561 acgaaccgct ccgccgcggc cgccgccgcg attgcagaaa taaaagcctc cagcacagcc
     1621 tgcacggggc ggccgcgttc ctgaaggctt gcccacaccg cgcccagcac gccgccggcc
     1681 gccgcaaaca gcaaaccgaa ctgcaagaac cccattcacg acccctcatc cgccaaaccc
     1741 cgcgactttt gcgccgaaaa caaaaacctc aacgcattat tccccgccag cgcgcagaaa
     1801 aacgccaaca gcggcggaac gaccataccc gtgttcaaag gcgggtacgc cccccaaaac
     1861 gccaccgaaa ccaaaaacca cacaaagccc gaaaacaaaa gcaggtaggc cgaagccaca
     1921 caagccctca cgccccgcgc aaacagcaat gccgtctgaa gcagccccgc cgccgccagc
     1981 acccccaccg tccacgcctc cggaatatcc tggaatttgt aataaatccg ccacgcgtaa
     2041 atctcgtcgg gcgcaagcgc gaacaccgcc gcgtaacccg acaagcccaa accgctgacc
     2101 gcctccagca cccgcgtcgc cgtgccgaac aaccacgcct gaaacccggc gggcaaaaac
     2161 cgccgattca acaaaaccct caacataccc gtcacacacc ccccggcaaa acaccgcgaa
     2221 gccgaagccc cgcagtgtcc gacgccgtct gaaaacttat tccgcgtaaa ccggttgcgc
     2281 gtctttgggg atgcccgccg caccttccaa cgccttgcgg tacacccaac cggggtcggg
     2341 cgcggttatg tccgtttcct gaaaatccaa tgtgcgcgcc aacaggtagc gtttgcccga
     2401 tttaaacgca ttttccgaca catagccgtt caacgtcgcc gtaacataac cgttgcggta
     2461 atccgcgccg atatgttcga cgacgtggta ggccgcactc gcgccggtca taccgtcctc
     2521 gactgcaaaa ttcaaggcaa caatcttttt atcttcaatc atttcaaatc tccaaaagcc
     2581 cgctaggggc gggaacgaaa atgccgtccg aggtcttcag agggcattgc ccggacaaaa
     2641 caggaaaaac cgcagagccg gcatacgggg gcgtcagcca acctgccaaa cctcgttttc
     2701 gcgcgattcc ggaatcagca ccctgatatt gcgcagctcg atatactgcc accagtgttc
     2761 cacaaaaaac agcaggttgg atttcggaac ggcagtatta aactcctcct gaaacagaga
     2821 atactgttgg ggcgcatgac cccggtattt cttaaccact acaaacttat ccgacctgtc
     2881 gaacgcaagc atcgtactcc agtcggcatt ggtaaaaggc cggtactcga acgatatgcc
     2941 gtagatatcg tccggcaact gaagcagccc gccgtaagca ttccaatgtt tatacatctg
     3001 attttggtaa tcgtgtacac gccggtcgcc gcccaaataa acctgcttct tactgtcgta
     3061 ccggaaactt tccgcaaagg tgcgccaaac catcctcccg agcagctgct tgtattcgta
     3121 gtcggattgc gccaaatacg aaaccgacac gaacggcgaa aacaccgatt cgtgggaagc
     3181 gagcgacaac ttcaggctga cgggtttgcc cgcggggatg acttggatct ggtactcgag
     3241 gcgggttctg taccttgagc cggattgttg ggtataatcc ccgccccccc ttgcccgggg
     3301 gggcaggtct tcgtacctcc cgaaagttcg gccgcgccaa atataattgg tatagttctc
     3361 ggtttccctg acttccctcg gcgcaaacac acccccgttc aacagcagct ttgcctgcac
     3421 cctgttgttg ccgaacgttt ccgaaaccac ataaaaattc ggaatcaaaa ccctcggcag
     3481 cgggtcttta tccaaatcca acacccaaac gttaccctcc gtccaacgca gcctgtgcgc
     3541 cctcaacacg tcgccctcga cggacgaagc cctgaccgtg ccgttgaaat aacccgaatc
     3601 ggcctcaatc cgtccgcgta ttaccgcatt gtcggccgtc aggatgccgt ctgaagacac
     3661 cgtaaaatta cccccgccga tattcaggct gccgcccgtg atgcttccga gattggaaga
     3721 taccgcgctc aaatcggcaa cctgaatctt atcggcagtc accgcgtttg ccccgatttt
     3781 atcggcggta acggcggcag ccccgatttc ccgcgccgta acgcttccgg ctttcaggcg
     3841 gtttgcgtcc agcgtgtccg ccgcgatttt gccgccgtgg atatccccgg cgttcaactt
     3901 atcgacaacc gccttgccgt tgaccaccaa ttcgccgttg atgcccacgc ggtttttctg
     3961 cgtgtccacc acgaacggga acacatccgc cttcccggcc gcaccgatgc cgaagcggtc
     4021 ggcattcacg ataaacttgc tttcgggcgt gccgtttttc ggcgtagtcg ccaagccgaa
     4081 gcccgccact ttgccgtttg catcaacctt gaccgtgtat tgcgcctcca agccgttgat
     4141 gcttcgggcg tgtgtctgaa ccgccgccgc attgccgtcc gccgtcgatc ggactgcctc
     4201 gacgcgctcg ttgacgctgc ccgcgccgtt gccgtcaatc aggttgattt tgtcgcgcaa
     4261 agccttgttc agactgcttt ccgacaggtc ggttgccgat acgtcgtaaa cggtaaacga
     4321 tatgctgccg ctgattttca ggttatcttt gccgaaactg tcgtaacccg ccgccctcaa
     4381 gtaataggtc tttcctttct ccagcggttt gccgccgcat ttggcgatgg ttataaacgt
     4441 ttccgcgccg tcataggctt tgtttgcgtc cgcggccggg caggctgcgt tttcgcaaac
     4501 ccagacgata attcctgcaa agtcttcttc gtcaggtttt tggcaggtaa aaaacgcctg
     4561 tttcaaaccg ctgtcgatgg aaataccttg taatgcctgc agttgcggat tttgcgccgc
     4621 gatttgcgcc caattgcccg ttttaccggt aacggcacgc ccgcgaacct tgaagacaat
     4681 accgcgtacc tggccgccgt ccgccttcat atccgcctga gtgtaggtgt agctgttgtc
     4741 aacgatgccg tcaaccgcac gcagacggcg tcggctgccg cctgcgtaga tttccacgtc
     4801 gtaggtatcc gcgccgtcca atttatccca agcgatgacg gcttctttgc cgtatgccca
     4861 cgatgatgtc aggcgcaggt tttggatttg accgagcggc gcgccttcga tggtgtagga
     4921 atacgccggc acttccgcca aatcctgaat gccgccgccg aaaacgttgt acgaaaccag
     4981 tttgacccaa accgtgcggc caatccagtt gcgcggcacg gcgtatttga acaatgcttc
     5041 gtcgatgcgc gcaaaccggc tgcccgccgc gtgtgcgttg acggcggagc cgtacgcgcc
     5101 gcgcgtcagg ttgcccaatg tgtagcgtcc cacgcccttc agttcggcgt cggcgtatgc
     5161 cagaaattcg ccgtcgacgt agcacaatgt cagcaaatcg cggctgtcct gctccgtgcc
     5221 gcccgtcagc tgccccgccg aaatttccac gcccaacgta tttgtgcggt cgaaaaccgc
     5281 gccgtcgggc aaatcggcgg tcagcgcgcc gaaacgcgcc ttgcggttga ccgcgccgac
     5341 gcgggtgtag ctgtcgccgt ccgccgatac ccacacttcg gcgccgcccc acataccgcc
     5401 cccggcggtt gccagccaga tttgcggttc gccgcccgtc agttgcaacg gcgcttcgaa
     5461 aattaccggc acatgggcgt tgcccggcga aacgttgtag tcggccgaat aacccgaaga
     5521 cggctgcgtc ggatattccg acacggtata aacaccggcc gggcagtctt cggccttgac
     5581 ggataaaacc ccttctccgt cttcttcgat ttccgtgatt cggacgggcg ttttattcag
     5641 gccgagcccc gcgtcggtca gggttacgat gtccatcggc tcaagcaggc agtatttcca
     5701 acccagctta aactcatatt cgttgcgcac gtacagggcg cgttgcagca gttgttgcgc
     5761 cactttttgc gccaccttcg catcgcaaat gccgtgcatc ctgaccgcgt ctttcgggcg
     5821 caatccgtac tgctcgatat tcgcctggtc tttcgcttcg gcgatggcga cgttgtagtc
     5881 gttgtccctg tcaaggtact cgacctgaat ctgattaaac gcatcggcat tggttttgcg
     5941 cccggccttt acagggtctt gcgcgcccga aacgatgaaa tcgtcatcgg tcaggtcgta
     6001 ggcggcctta ttgtcggcaa cgtacaccgc gccgttcccc gaatggctgc cgtccccgta
     6061 ggggatgatt ttcaggcggc cttgcgaaaa taccgccgcg ctgttggtct gctccagcag
     6121 ttcggaaata ttccgttgcg cctccccctg ttccgtgtag gcagggctta ggaaaatccc
     6181 gacggcgcgg caataattgc tgtaccggtc ggtatcgccg atgttttggg acgggaaacc
     6241 gcagccgtag cgttggttcg tcagcaaatc caatacgatt tcgcgcgggt ttgcatcggg
     6301 gatgttgccg gaatagcccg atttcccgat gacctcgaaa ttgtgctgat atatctgcgc
     6361 cgatttcgtc agttcgtagt tcgggctgca caaataagcc gtgcccgaat aattcaaggc
     6421 ttggccttgg tgcttcgcct gttgcaggtg cgtccacaac ggctgctcgt cgccgccgcg
     6481 cataagcgtc aggcgcaact gtgccggcga atcgaatttc tccttgtcgc gccaaacccg
     6541 ccccacgccc tgaatctcgc cctcgcacaa agcaagcatg acggcggctt cgtaggtata
     6601 ggaaataccg acctgtttca caccgccgcc gcccttgccg ccccggcgcg tcgtagtttt
     6661 atgctcgatg gtgacgaaat cgccgtacca aatcaaattg cccgccacgc gcgtcctgcc
     6721 gtagatgacg ggcagggtca gcccctgtga tgaccgctga acctgtaacg ataaaatccg
     6781 ctcttcggca gatgtaacgg ttgacgattt accacccata aaaccacctc gaaacttgca
     6841 aaaattatca aaatcgatat aatttactta tatcaaaaca gatataaaat atgaaaccgt
     6901 taaatttttc aggcggttca ttggattgcc tgcggcaatt ccccgaaaac gccaaacagg
     6961 cggcaggtta tcaacttcaa gaaaagccgg aaaaccgcgg gcgccgattc ggaatcggcg
     7021 gaaaaacgtt acaacaaatt gattcgggaa aagaaataat ggaaagccaa actttcgcct
     7081 ccgtatttga cgcactgtgc gacacgcctg cccaagccgc caatatgcgg ttgcgcgccg
     7141 gcctgatgat gcacatcgcc gataccgtcc gcgaaaacgg ttggacgcaa aaacaggccg
     7201 cagaacattg cggcctgacc cggccgcgca tcaacgacct gctgaacggg aaaatcgaca
     7261 aattttcatt ggatgcgctc gtaaacatca atgccggact cgggcagtgc atttccttat
     7321 ccttcgcccc cgcgtaaagc ccgtcctttc agacggccgc aaaatcaaaa tgaaaatgta
     7381 aaaaaccgaa ccccgcgccc gataagttcg gcttggccga tgtcgtccga caccacgccg
     7441 cgcccgatgt agctgtgaat aaccttgccg ccgcccgcca atatgccgcc gtgcgaaaac
     7501 gaccgcccga aacgccatac tgcaatgtcg cccgcctgcg gcgattccgt ttcacggcaa
     7561 aattgcgtga cgaaccccaa ataacgctcg caatcgcggt gcagatgcca atcttgaggg
     7621 taagggcgcg ggtcgaaccc ttcgggaagc agtccgaccg ccccgtagac ggcgaccagg
     7681 agcatcgcgc aatcaacacc cgcgcccttg accattgcat gatgatggta cggcgtgcca
     7741 agccacgacc gcgcctcttc gacgatccgc gccctcaaat ccgtttcgga cggcatatca
     7801 caccaccgta tctgccgaag ggatgaacgg gaagccgcgg aaatgcacga tattgtcaaa
     7861 cttatccttg caggtatcgc gccgcttgtc gcagcccgga taaaccttga acgcatcgcc
     7921 cgcctgcggc gggtagggca ggcgaagggc aagttcgaac gtgttgccgc cgtgtgcctt
     7981 gaccgtcctg ctcaaacccg cgttgcgccc gcccgcaaac ttaatcacgc cctgcgaaaa
     8041 ccagccgtca ggctgcgtca gattgtgctt cagcacggtt cccgtccggc tgttttcggt
     8101 tacgcgcccg tttaccgtga atttctcacg gttgaccttg cagccgtcgt catagagcgt
     8161 cctcatgcag cccgcctgat aaatgttgcg cgggctggaa acgttcaaaa gctcgatgtc
     8221 ggatttcacg tcaaccttca cggacgacct gccgcccgat acgtccgaca cgcgccctga
     8281 aaaaatattc accgcaccga cggggcgcag tccggcatca aaaaacacac ggtctatcct
     8341 gacccgcgcg ccgtccaata cgccgcccag ggcggcctcc gcccattgca ggccctcaag
     8401 cctgtaatcg ggcgcggctg aaatctgcag ggtgttggaa tccacttcca atccgacggc
     8461 ggtacgggtt gcgccgcgct tgataaccag ctcgtgcgcc ccgtaggcct gaccgtccca
     8521 aacgacgggc atatccgcgc cggtatgccg cagcacccgg ccgcccgaaa gcgtaatggt
     8581 gtacaaatcc gccatctgaa actcgtcgcc gccgtgcagc aaatcaatca gttcttttgt
     8641 cgccgccttc atagtttcac actcgtaaac tcaatctttt tggccgccca caagctgccc
     8701 aaaacgtttt caaaatccac cgtgtcagac gtaaacctca cgcggaaata aaagccgccc
     8761 gtccatgtga tcgggcggcc cggcggttgc ggcgtgttga aaaccaaaac gcccttgtcg
     8821 gtaacggtgt aatcgcgccc gtacgccaac gccgtgccgc cgaccttgac ggcgggccgt
     8881 tccttgaccg ccgacacagg ctcgataaat ccgcccatcg aacggacaag ctgataacgc
     8941 gcaacgccct gcaccgtgtt tccgacaggc tggtcggtta cggcgttgtc ggccgggtct
     9001 tcgtaaagga aactttcaaa gctgcctttg cgggcgttga agaatcccgc cagtttttcc
     9061 aactcgttta cggacgcttt tgtccgcaat acctcgaacg acagcgaaaa ccgccattgc
     9121 gggtaggtgt agtaggcggt tcggaactca cggccgcttg ccgatttctg cgtccccgta
     9181 ctccataccg ccgttttctt ccgcccccac ttcaagccgg ggaactcggg gaaaaccgca
     9241 ttgcccatca gatgattcct ttcgctttga gcagtgcgtc aaattcgtct tcggacagtt
     9301 cgccgccgcc aagcataccg acggcttcgg cttcgtccgc ttcgctttgc acactgcccg
     9361 acgacggctt gacgcccata tacgacgctg ccaagatatg cacgggcgga tgttcgcgcc
     9421 aatactcgtt caggtgtttg atacgcggca aatccaagtt gtcggcgacg tagtcccacg
     9481 tccagccggt agaggcgcaa acgtgggcaa tcaccgcgcc gaaacttaat ccgccgccct
     9541 ggcctccccc gcttgtgcgg cttcctgctc cctgcgtttc aagcccgaaa cgtccatcac
     9601 ggcggcaaac acttcgttca tgttgccgat atcgatcaaa tcggccgctt cttcgcgcgt
     9661 taaatccggg taattgcgct tcagggcggc gtgggcgcaa tcgataacgg tagagatttg
     9721 tccggcatcc gccgcattgc cgtcaaatga gccgatgcgc tcctgcaact gctccaacgc
     9781 gcccagcgca ataggcggaa tcacataatc cgcgccgttc agttctacgg ttacgccttt
     9841 aatccgtacg gtcattttta tttccttgac tttggttgaa taaaaaaagc cgccttttca
     9901 gacggcatat aaacattact cctgaatcca cagcgtaccg attttaaagc ccgcctcgtc
     9961 ggttgaggcg gtaaagtcga tttcggggac ggagaagtcg tcgtttttag tcgagaacaa
    10021 gcccaatttg ccgctggtta cgctttccag ttccaacagg gcttttttgc ctttgaactg
    10081 cgtcaggtac ctcatcttaa acgtcggcgt gttgcccgtc gccatattgg tcagctcgat
    10141 tttcttcgcc gggggcatcg tttgcgtata ggtaaagctc ggataaacgg ttttgccttt
    10201 atccgagctg ttaaaggcgt acacgcccga ttccgaaacc gtatattggc cttgtttcgg
    10261 ggaggatgct actttgatat aagccgtgcc gtcctggccc atcacgcccg cgtcttcggc
    10321 gaacttgccg ccgccgggcg cggctgcctg aaggtaagaa taattgtcgc catcgaggac
    10381 tttgcccgta gtttccgccc aaagcgcctt catcgtgccg gttgcatatt ccgtaccgaa
    10441 aaacagggta ttgagggtca ggccgttaat caacgcgcct ttcattttgc ccgacacctt
    10501 gaccttgcct tgcgccacag ccaaaggata acggttttga ccgtagaact ctttcaattc
    10561 cgccgacaaa tcgacggaca tttcctgcaa gcccataatc cgcacgggcg ttgcgttctg
    10621 tacacggttg ccgtaagcat ccgtaatcat ttgggcgaac acttcgccgc tgccaaacgt
    10681 caactgcatg acatcccttt caaaaaaagc cgcattacgc ggcgcaaatc acaatcggaa
    10741 taatacaaac cgcctgttcg ccaagcgtcc cctcgtcggt ttccaccgta ccctcgacgc
    10801 ggcaatactc gatatccgcg ccgtccgccg ttaaatccgt cttgcccgta acggggtgga
    10861 cggcgttcac ggcattgcac accgcgtcaa tcagcggatt catgacgggc gcgggcggtt
    10921 ggcccgccgt ctggacgtac aggtaaacat cggcgcgcaa aatccacttg gtctcccgcc
    10981 ccgtcaatgt caacgcctgc atatcgccct gcgccataaa taacgcgggc tgatcgtagc
    11041 gtttcacatc gttccagtgc accagcttcc ggctcttggt tacgaagccg tccaatgcgt
    11101 ccagcttcgc ccacagcgcg gaataaaccg cctcacgatt catttcaaca cctttccaat
    11161 cgatttttgc aggtcgtccg cgaacttcgg cgtcaaatcg cgcaatgccg aacggaggaa
    11221 cgaccgctcc ggcaacttca catcacgggt atgggcgcgg acgcggacat atcgcggcgg
    11281 tttcagcggc ttgccgaacg cctgcctgac ctggcgcagc gaagccttaa cgttgacact
    11341 gcccgcgaaa ccatactcat gcgcgatgcc gtaacggacg ttcgtatcga cttcgcccga
    11401 aacgacattg ccggaaacgt tcacgcgctg gtgtacggag cgtcgcaggt tccccgtcct
    11461 taccctcagc acccggcccg ataaccggtt cagcataact tcgctttgca ggcgcaatgc
    11521 cgacctgccc accgatttga cgaccgcatc ttgaacatcg gcggcatatg ctttaaaaac
    11581 cgccgccaga ataccgccgc cgataaattc catcttcagc atcacacgcc ctttcgtttg
    11641 tactcattga gtatcgcaaa cgccgacggc ggcataccgc cggaattacc gaacgtagaa
    11701 aaagcgacgg tttcgcctgc aagcgtcttg ctccgtacgc ccttgccctc gatttcgttc
    11761 acgcgctgca ccgcgataat catcaccgct tcgcgtatgt cggcgggcat gcgctcataa
    11821 ccggcgcggt acgatacctc gacatttttc agcccccgcg cgaaaccttc ggggcgcatc
    11881 agcagccagt tgtcaaactc ccagccgccg gcatccgcgc cgttgatttt gacggacgac
    11941 accgacagga cgggataatt gtccaacacg atgcggtctt tgccgttgcc gtcgtaccgc
    12001 ccgacataat cggcggccag gaggctgcgc ccgatatagg cttccgccgc cgccgacacg
    12061 ccgtcaatga cgctgaggaa gaaatcgtcc cgccggtcat gttcaacgcc gatacgctgc
    12121 ttgaactcct caagcgatac cggggcggcc atcgttattc agccttttcg gcttcggcag
    12181 gttcggcttc tgcttcggca ggttcggcag cttcggcttc ggcttcggca ggttcggcag
    12241 cttcggcagg ttcggattgt cctgccggct gcccggtttt aggtttacgc ccgcgcttgg
    12301 ctttttcagg cccttcggca ggctcggagg caacgttgcc aaaaccgaac tgatacagga
    12361 attccgccgc ctccgacggc acttccacga taccgttttc atctgccgcg tagctttggc
    12421 tgccgaagga aacgtcggta aacccttcgg gcgcttttaa tttgactgtc atctcaaaat
    12481 ctccaaaaga aaaagccgtc tgattttcag acggcatatc ggcttaaccc acgttggtaa
    12541 tcataccgaa ggcaggcatg aacatacctt gcagcacctc gtccgcatag acgccgtatt
    12601 cgtacatacg ggtgcgcagc ggccattcga tttggtaata ctcttggcgc gtacgcactt
    12661 gcagcagatt gccgacgccc tgaacgtagg cgggcaggcg gcttgagtag aacaggtaag
    12721 taccggcagg caggttcggg tgtaccacga tgttcaggtc gtcgcccgtg atcttgttca
    12781 tatacgaacc gacaacgaca ccggcgcgga tgttcgccgt attgttcacg tcaacgttca
    12841 acttaatcaa cggcgcgccg ctgtttccga taatcagctt ggtcagcgcc gccaaatcgc
    12901 gggcgttgac gtagacggta tcgggggaca ggcggtatcg ggtaaagaaa tgcgcgaacg
    12961 cctcttcaaa ttcatacacg ccgcccgcgt tgtcggaagt caggccgccg cctttgttgt
    13021 ccgaccagaa cgcgcccgaa tcaggcaggg cgatttgggt caacaggccg tcaaattcca
    13081 aaatagaagt ggaattgtct tcggacggca gggaagcggc ggtctgggta ccttcggcat
    13141 cagccgaaat ttccactttg gcggcagtgg taaccgcgcc cagtttttca gaaccggccg
    13201 caccccagta ccaagcatag gcaaccgcgc cgcgaacagc cggaatcacg gcggttactt
    13261 ttttgcctgc ctcaataccg gaaacggaag ccgccgcaga ttttcgggcg gaaccgccgc
    13321 cgaacgtatc ggttgtacca tccgcgtttc ggcgtgtgat tttggcaggg acttgggcag
    13381 ttttaatgtt cgggctttga ccggttgcac cgttgttcgc gcctgccaca tcccagtatg
    13441 cctgcaagcc caaagccaca cagattacag acaaggtgct gacgccgagt ttgcccgccg
    13501 cgtcggcgga aacgacggcg gtcggtgtag gtgtaacgcc tgccttcagg ctggtgttgc
    13561 cgcccagcaa aatcatttct tcggcaacca tagtagcctg aagggtttgg gcgaccgcca
    13621 acgctttcac gtcctcgaaa ccgcgcgcgg cgtaatccgc ttcaaaggtt acttggtttt
    13681 ccaagccgat ggcgcggaat tgcgcgttgc gttccaccgt ttcgtgattg atgacgccgc
    13741 cgcgcctgcc ttcgccgata ccggcgcgtt ggttgccgac gttgatattc gtgacggctt
    13801 tccagttcga gccgatggcg cggccgccgc ccacgcgcgg gatacggttg cgcaacgggg
    13861 tcaataccgg gtagagtttt tgagacggcg cggaaaggtc ataggtttgc aggccggcgg
    13921 taaagccggc cggctgggta aaacctttgt caggcggccc gccgcccgct tgtgccgact
    13981 tcatcagttc aatcgtttct tgtgtgagtt ggttcacgtt catttatcgc tcctgataat
    14041 aaaaaaaccg cctgtaagcg gtgttacaga cggcctgttt gttttgcctt aatgagtgtc
    14101 gccacgtcat ccagcgtgcc gtcattcttt acaatcggct gaaaaccttt taacgggtct
    14161 tcgccgttat cttctgcctt actgatagct ttcgtactgc ctttcggcgg tactgcctgt
    14221 ttcttcaggc tttcgatttc cgcctgcgct ttggcaaggg cgtcattcga ttttttcagc
    14281 gcgtcttgtg cctttgccag ttcgtcagcc gattccgctt tggcaagatc gcctgatttg
    14341 ccggctttgg ccgccaaacc gtcggccggc ttatcggctt cgcttgccgc caacgctttc
    14401 agcgattcgg caaggccggc cgccgattct ttgatttgcg cggtaacggc ttcatcgatg
    14461 ttgtcgtagg atgcgtcctc aaccagccat ttcagcgaca tcaatacatc agccggtgat
    14521 ttgacttggt acattgattt ggcggccgtc ccgcctttcg gcttgtcggc tttagccaat
    14581 accgctttca aggcggcgat ttcagattcg gacaaattca cgcttgccga ttttccggct
    14641 tcgtcctttt tgccggcgtc cttatcgcct ttatcttcgg ttttaggccc tttatcgccg
    14701 tctttaggat tttcatcttc ctcttcggtt ggtttgtcag acggcttgcc gtctttatcc
    14761 gccgcccctg cctcatcttt cggtttgtcc gccttaaagc aggtaaacac cgcgtcggga
    14821 ttggcagggc ggtcgacaag gctgatttcc gtcagcttca agcccgtgat ttgcgacttg
    14881 ttcaaatcat cgcgggcggt aaccctgccg ccgatggaaa agcctttgta aacgcctgtc
    14941 ttgactttcg tcacggcaac agggtcaacg atatgcgccc cgaaaaatgt gcgcccgtcg
    15001 tcttccacgt tgatttcaat cgccgttccc gccgcgtttg agccgtgcat ttcacgcacc
    15061 gcgccgaact tcatataatc gggaatcgcc gccttcattg cttccgccgc gacgacttcg
    15121 ccgtccgaat cgaccgcttc gctcgaggca tagccccaaa ctttgaccgt gccgtcgtcc
    15181 tgcgcctccg tcttggcgat ttgcgcgtat aacttcgtca ttggtttgct ccaaaaaaaa
    15241 gccgccccgc gaaggggcgg caaaacacac ccgcctgacc cgaaggaatc aagattcagg
    15301 catatcccct gccaaaacag gcacaaccgc gcaccggcag ttcggatga
//
LOCUS       DAXDXW010000062        14849 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXDXW010000062 DAXDXW010000000
VERSION     DAXDXW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14849
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.063"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..221)
                     /locus_tag="ACFB64_001592"
     CDS             complement(<1..221)
                     /locus_tag="ACFB64_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC1209926.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENSRSELAAPVYIQTGATSFIPIPTEI"
     gene            386..1309
                     /locus_tag="ACFB64_001593"
     CDS             386..1309
                     /locus_tag="ACFB64_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC1209927.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFLG
                     ADNWRRRLNSGFVVGSDGAALDEHSLFEPGKTMFYYRETGCESEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPGGRFLRETLLTRLRLHPELQHLNVEDITPIHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLARGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDAFYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(1388..2029)
                     /locus_tag="ACFB64_001594"
     CDS             complement(1388..2029)
                     /locus_tag="ACFB64_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC1209928.1"
                     /translation="MLKPETIHIPGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQVRIPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIEKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(2084..2875)
                     /locus_tag="ACFB64_001595"
     CDS             complement(2084..2875)
                     /locus_tag="ACFB64_001595"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC1209929.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     RPSELVLSYSRAMMGWLLFAERLPQHITQIGLGGGSFARWTDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGGKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            3041..3116
                     /locus_tag="ACFB64_001596"
     tRNA            3041..3116
                     /locus_tag="ACFB64_001596"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3074..3076,aa:Thr,seq:tgt)
     gene            3258..4049
                     /gene="panB"
                     /locus_tag="ACFB64_001597"
     CDS             3258..4049
                     /gene="panB"
                     /locus_tag="ACFB64_001597"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC1209930.1"
                     /translation="MITVNTLQKMKAAGEKIVMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDEAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMRGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            4172..5008
                     /gene="panC"
                     /locus_tag="ACFB64_001598"
     CDS             4172..5008
                     /gene="panC"
                     /locus_tag="ACFB64_001598"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC1209931.1"
                     /translation="MQIIHTIRELRTWRENIGKVAFVPTMGNLHEGHLALVREARKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLAAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPHLQNELCGKFRPGHFRGVATVVSKLFNIVLPDVACFGKKDYQQLAVIKGLTED
                     LNFDIEIVPVDTGRAADGLALSSRNRYLSVGERAEAPRLYRELQAVAESLKQGGLDYA
                     GLERQAADHLTAAGWLVDYVEIRRADTLEMARAGDKKLVVLAAARLGTTRLIDNVEVG
                     LP"
     gene            5335..7173
                     /locus_tag="ACFB64_001599"
     CDS             5335..7173
                     /locus_tag="ACFB64_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC1209932.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADVELPKEVGKVLRKHRR
                     YSEEEIKNERARLAAVGERVNRVFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLREGGNQHLDGLEEV
                     LAQSDDVQKRRIFLLLVQAAVQQGGVAQKASKAVRRAALKYEHLPEAAVADAVFGVQG
                     REKEKAIEALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLRKPDDAYARLNVLLEHNPNANLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAAELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALIGLNNIIAKLSAAGST
                     EPLAEALAQRSIIYEQFGKRGKMIADLETALKLTPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRYGIALPEPSRKPRK"
     gene            7313..7894
                     /locus_tag="ACFB64_001600"
     CDS             7313..7894
                     /locus_tag="ACFB64_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC1209933.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGGTLGQLCQDRDGALAVDGKGNVYQAEGTE
                     DLSRQLVGFKLPIQYLHIWAEGRRVAGAPYRIRSDGILEQYGWTIGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            7904..8749
                     /gene="ispE"
                     /locus_tag="ACFB64_001601"
     CDS             7904..8749
                     /gene="ispE"
                     /locus_tag="ACFB64_001601"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC1209934.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVGGIPQEADLSYRAASLLQKYARNLAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQWQLIDLGAALGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTYEGLTRDSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSKYGSAMMTGSGACIFAAFQARNSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            8757..8832
                     /locus_tag="ACFB64_001602"
     tRNA            8757..8832
                     /locus_tag="ACFB64_001602"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8790..8792,aa:Gln,seq:ttg)
     gene            8845..8920
                     /locus_tag="ACFB64_001603"
     tRNA            8845..8920
                     /locus_tag="ACFB64_001603"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8878..8880,aa:Gln,seq:ttg)
     gene            9088..10071
                     /locus_tag="ACFB64_001604"
     CDS             9088..10071
                     /locus_tag="ACFB64_001604"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC1209935.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGKTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            10138..10710
                     /locus_tag="ACFB64_001605"
     CDS             10138..10710
                     /locus_tag="ACFB64_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC1209936.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTAVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(10836..12005)
                     /locus_tag="ACFB64_001606"
     CDS             complement(10836..12005)
                     /locus_tag="ACFB64_001606"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC1209937.1"
                     /translation="MTAHKILPVLLPIILGVSHATAASPAPNRPTVHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNTNTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALCANDAALTLADRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLGREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFENIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNRALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGHTIAEKEIVALENVEKRSRWQRLWTRLTGQ"
     gene            12154..13680
                     /gene="ilvA"
                     /locus_tag="ACFB64_001607"
     CDS             12154..13680
                     /gene="ilvA"
                     /locus_tag="ACFB64_001607"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC1209938.1"
                     /translation="MNTPLPYSDYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNGTFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTNNEIAKIHIRYMVGGRTDKVGHERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(13736..14812)
                     /locus_tag="ACFB64_001608"
     CDS             complement(13736..14812)
                     /locus_tag="ACFB64_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC1209939.1"
                     /translation="MSITIQNLNKKRFGNFHALKNINLNVPAGKLVSLLGPSGCGKTT
                     LLRIIAGLENADGGQILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTV
                     LPKPERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLL
                     LDEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQ
                     TGSAEAIYRKPENAFVTEFLGETDAFEGRIEKGIWHYNGFAWKLDAQYKWQEQTATGY
                     IRPHEWQIAAEHETPMIRAEIEKIHAVGALTHVLVKHGKQDVHITLAGSDAARYPIAE
                     GKKLNLIPKQVYVFSQNELIEYSI"
BASE COUNT         3373 a         3474 c         4335 g         3667 t
ORIGIN      
        1 tgaatttcgg tcggaatggg gataaacgag gttgcgccgg tttgaatgta aaccggtgcg
       61 gcaagttcgg aacggctgtt ttcgctgttc aggtaggtaa gggaagtttc cagtttccat
      121 ttgcccttgt cggtcattat gtcttcaatc gtcaagggca ggtcggcata agcggataaa
      181 ggcaggatgg cgggcaaggc gggcaaaaag atacgtttca tatttcctct tattgatatt
      241 aattcttatt taataatagt aatgaatatc cgataattct gtctaaacaa atttttaaat
      301 caaaaaaaac gatatatttg tttttatcaa ttatgttttg atatgccgtc tgaaaagttt
      361 gtgaaaaacg gttacaatcc gccgtatgaa aaaacgcaat aatcctcttc cgctgttgaa
      421 cggtgtcaaa cccagttatt tggtgctgcc gcatgaaaag cagttttacg ggctgccgct
      481 gctgcatttt ttgtgcatcc gctttccttt tttgggagcg gacaattggc gcaggcggtt
      541 gaacagcggt tttgtggtcg gttcggatgg tgcggcgttg gatgaacatt ctttgttcga
      601 gccgggtaag acgatgtttt attaccgtga aaccggttgt gagagcgagc cgcgtattcc
      661 gtttgaagaa aaaattttgc atattgatga gcatttgatt gtggtggaca agccgcattt
      721 tctgcccgtc atccccggcg ggcgtttttt gcgggaaacg ctgctcacac gtctgcgcct
      781 gcaccctgaa ttgcagcatt tgaatgttga agacattacg ccgattcacc gcttggacaa
      841 ggatacggcg ggtgtgatgc tgctgtcgca caatcctgcc acgcgcgggg cttatcagac
      901 gatgtttcaa aacaaaacgg tatggaaaac gtatgaggcg cttgcgccga caaggacgga
      961 tttgccgtat ccgctcgatg tggtttcgcg tttggcgagg ggggagaaat tttttacgac
     1021 gcaagaggcc gaaggcgaac caaacgcaca tacgacggtc gaactgattg aaaacagggg
     1081 ggaattcagc ctttaccgcc tcacgccgca taccggcaag aaacaccagt tacgcgtgca
     1141 tatgatgggt ttgggtatgc cgctgttgaa tgacgcgttc tatcccgtgc cgtctgaagc
     1201 gggtagcgag gattatcgga aacctttgaa gcttttggca aaaaagattg cgtttgcaga
     1261 tcctttgtcg ggtcgggaaa gggtgttttg cagcgggttt tgcctgtaat gagagggaac
     1321 gcaaacccgt atcggcagat gccgccggat acgggtttga tattatcgat gctgtctgaa
     1381 aggggcttta tccgttcagg catacgggtg cgaagcggag gatggtgtcg cgcaggacga
     1441 tgagtttgcc gtgaaagaag tgcgtcgaac cggcgatggt aatgacgggc aaatcttgcg
     1501 gttctgccca tttcagtgct ttttcgattt cgacgacctc atcttccgcg ccgtgtatca
     1561 tcagcgtttt ggcaacgttg ggaacggcgg acggctcggg gcggtcggta tagtggcaga
     1621 ctgccgcacc gatgagcagc aataaatcgg gtatgcgcac ttgtgcggca aatgtggcga
     1681 catagccgcc gaaggaaaag ccggataagg caaattccgg ggcttcggga tgttgggcgc
     1741 gggcatagtc gatgacggcg aggcagtctt gcgtttcgcc gcgtccgtaa tcatgtgtgc
     1801 cttcgctgcc gcctacgccg cgaaggttgg gaaggtagca gtggaagccg agcttgctca
     1861 atgctttggc ggcggtttgg atgactttgt tggtgttcgt cccgccttgg agggggttgg
     1921 ggtggttgat gacggcaaca ccgcgtgccg gtacttgttc ggacgggata tggatggttt
     1981 ctaaaatgcc ggcagggccg ggtatgtgta tggtttcggg tttcagcata atggtttcct
     2041 cttgattttc cgcatattcc gatgcagccg cgaagatggc ggcttaaagg tggaaatgct
     2101 tgccgttgtt ggggttggac gctttcaggc cggcgagcat gcggtggaag tccaatccgt
     2161 atgcgttgct cagtttgtcg gcacgttttt tgagtttgtc tatgttttgc tctttggggc
     2221 tgctttggaa ggccattacc gcgacattgc cgtggctttc ggcaggaagt tccaatacgc
     2281 gcccttcaaa aacgctcaac aaccgttcga tgaagcgttg atagcgtttg tcgccgctcc
     2341 accagttggt aacgaatatg ccgtctgaag agagtgcgtt gcggcagtct ctgaaaaacg
     2401 gttcttcaac cagcgtatcg atgatttgtt cgccgtcgaa tccgtccacc aagatgatat
     2461 cggtgttgtg gcggaagact ttgatgtatt ccgcaccgtc tgcttcgata atttcaaatt
     2521 ttccgccctc gaaaggcaac tcaaacaggt tgcgggcaat ggcgatgacc tgaggattga
     2581 tgtccacggc ggtttggcgc gtgtcgggca ggtaggtgtc tgtccagcgt gcaaacgaac
     2641 cgccgcccaa gccgatttgg gtgatatgtt gcggcaggcg ttccgcaaac agcagccagc
     2701 ccatcatcgc gcggctgtaa gagagcacca gctcggacgg gcggtcgagg ttcatcgagc
     2761 tttgaacggt gtcgctgccc aagtgaagcg aacggatatt gccttcttcg gaaataccga
     2821 cttcgggaaa gccggatttt gcaggacgca ggcggcggta gggatgtctt gccatcggtg
     2881 tgaacggtcg gtttgaaagg cggatatttt atcagaatgc cgatgcttgt tctgtttcaa
     2941 attaattttg ttttaaataa gatacttagt gtatttttat gagggggtcg aacaaatttc
     3001 ttgccaaagt gtgaagttgc ctgcataatt cgcctccttt gccggtatag ctcagttggt
     3061 agagcacctg acttgtaatc agggggtccc gagttcgact cttggtgccg gcaccagatt
     3121 tgacagcccg attgtgtaaa acagtcgggc tgttttgcat ttgcggcacg gtcggtggca
     3181 gggctgcggg catttcacta taatagcccg tttcaaactg aaaaggccgt ctgaaaaggg
     3241 cggggtaaca atatctgatg attactgtga acacactgca aaaaatgaag gcggccggcg
     3301 agaaaatcgt tatgctgacc gcttacgaat ccagttttgc cgcgctgatg gacgatgccg
     3361 gcgtggatgt gctgttggtc ggggattctt tgggaatggc ggttcagggg cgacagtcga
     3421 cgctgccggt cagcctgcgc gatatgtgct atcacaccga atgtgtagca cgcggtgcaa
     3481 aaaatgcgat gattgtcagc gatttgccgt ttggtgcata tcagcagagt aaggagcagg
     3541 cgtttgccgc cgccgccgaa ctgatggctg ccggcgcgca tatggtcaaa ctcgaaggcg
     3601 gcgtgtggat ggcggaaacg actgaatttc tgcaaatgcg cggaattccg gtctgtgccc
     3661 acatcggtct gaccccgcaa tccgtgtttg ccttcggcgg atataaagtt caggggcgcg
     3721 gcggcaaggc gcaggcgttg cttaacgatg ccaaggcgca tgatgaagcc ggagctgccg
     3781 tcgtactgat ggaatgcgtg ccggcggaac tggcaaaaaa ggtaactgaa actgtttcct
     3841 gtccgaccat cggcatcggg gcgggcgcgg attgcgacgg tcaggttttg gtgatgcacg
     3901 atatgctcgg tattttcccg ggcaaaacgg cgaaattcgt caaaaacttt atgcgcgggc
     3961 aaagcagcat ccaggctgcg gttcgggcgt atgttgccga agtcaaagcc aaaaccttcc
     4021 ctgctgcgga acatattttt gcagattgaa caaggtctgc tgccgatgcc gtctgaaagc
     4081 cgtttcagac ggcattttgt tttgccgtgc gcggcgcgta taatcggcgc gttttgtcgg
     4141 gcaggaagcc cgaaggataa ggattaccgt aatgcaaatc atacatacca ttcgagaact
     4201 gcgcacgtgg cgtgaaaata taggaaaggt ggcatttgtg ccgaccatgg gcaatctgca
     4261 tgaagggcat cttgcccttg ttcgcgaagc cagaaaacgc gccgacaacg ttgttgtcag
     4321 catattcgtc aaccgcttgc aattcggtca gggcgaggat ttcgacaaat atccgcgtac
     4381 tttgcaacag gatgcggaca aacttgccgc cgaaggcgtt gccgttgttt tcgcgcccga
     4441 tgagaaagaa ctctatccga acgtggaaca gcgttacaac gtcgaaccgc cccatttgca
     4501 aaacgaattg tgcggcaaat tccgccccgg ccattttcgc ggcgtggcga cggtggtgtc
     4561 caagctgttc aatatcgttt tgccggatgt tgcctgtttc ggcaaaaaag attaccagca
     4621 gcttgccgtg attaaaggtt tgaccgagga tttgaatttc gatatcgaaa ttgtccccgt
     4681 cgatacgggg cgggcggcgg acggtttggc actctccagc cgcaaccggt acttgagcgt
     4741 tggggaacgc gccgaagcac cgcgcctgta ccgcgagctg caggctgttg ccgaatcgct
     4801 gaagcagggc ggtttggatt atgcgggttt ggagcggcag gctgccgacc atttgaccgc
     4861 tgcgggctgg ctggtcgatt atgtggaaat ccgccgcgcc gatacgctcg aaatggcgcg
     4921 ggcgggagat aagaaactgg tggtcttggc cgccgcccgt ctggggacga cgcgtctgat
     4981 tgacaatgtg gaagtcggcc tgccttagcc cgccggagcc gggaatgccg cctgaagcgg
     5041 atttgcgttt cagacggcat tttgccgtcg gctgtgcttc ggaataatgt cggaaaatag
     5101 gcggaatgaa ataaatttct ttttgttcaa caaattaaac tgtcaaacca aataataagg
     5161 caaggattgt ccggcatcgc atacaggcaa accgacgcaa tctcaaaaca cagccctgcc
     5221 ttcgggcggg ttttgcaaat ctacaaaccg tttggcttgt gataaagtga cgccttgtct
     5281 agatactcaa tatgccgtct gaaggttcgg acggatgtcg gataaaggat gattatgtta
     5341 cccgcccgtt tcactatttt atctgtcctc gcagcagccc tgcttgccgg acaggcgtat
     5401 gctgccggcg cggcggatgt ggagctgccg aaggaagtcg gaaaggtttt aaggaaacat
     5461 cggcgttaca gcgaggaaga aatcaaaaac gaacgcgcac ggcttgcggc agtgggcgaa
     5521 cgggtcaaca gggtgtttac gctgttgggc ggtgaaacgg ctttgcagaa agggcaggcg
     5581 ggaacggctc tggcaaccta tatgctgatg ttggaacgca caaaatcccc cgaagtcgcc
     5641 gaacgcgcct tggaaatggc cgtgtcgctg aacgcgtttg aacaggcgga aatgatttat
     5701 cagaaatggc ggcagatcga gcctataccg ggtgaggcgc aaaaacgggc ggggtggctg
     5761 cggaacgtat tgagggaagg gggaaatcag catctggacg ggttggaaga ggtgctggcg
     5821 caatcggacg atgtgcaaaa acgcaggata tttttgctgc tggtgcaagc cgccgtgcag
     5881 cagggtgggg tggctcaaaa agcatcgaaa gcggttcgcc gtgcggcgtt gaagtatgaa
     5941 catctgcccg aagcggcggt tgccgatgcg gtgttcggcg tacagggacg cgaaaaggaa
     6001 aaggcaatcg aagctttgca gcgtttggcg aagctcgata cggaaatatt gccccccact
     6061 ttaatgacgt tgcgtctgac tgcacgcaaa tatcccgaaa tactcgacgg ctttttcgag
     6121 cagacagaca cccaaaacct ttcggccgtc tggcaggaaa tggaaattat gaatctggtt
     6181 tccctgcgta agccggatga tgcctatgcg cgtttgaacg tgctgttgga acacaacccg
     6241 aatgcaaacc tgtatattca ggcggcgata ttggcggcaa accgaaaaga aggtgcgtcc
     6301 gttatcgacg gctacgccga aaaggcatac ggcaggggga cgggggaaca gcggggcagg
     6361 gcggcaatga cggcggcgat gatatatgcc gaccgcaggg attacgccaa agtcaggcag
     6421 tggttgaaaa aagtgtccgc gccggaatac ctgttcgaca aaggcgtgct ggcggctgcg
     6481 gcggctgccg aattggacgg aggccgggcg gctttgcggc agatcggcag ggtgcggaaa
     6541 cttcccgaac agcaggggcg gtattttacg gcagacaatt tgtccaaaat acagatgctc
     6601 gccctgtcga agctgcccga caaacgggaa gccctgatcg ggctgaacaa catcatcgcc
     6661 aaactttcgg cggcgggaag cacggaacct ttggcggaag cattggcaca gcgttccatt
     6721 atttacgaac agttcggcaa acggggaaaa atgattgccg accttgaaac cgcgctcaaa
     6781 cttacgcccg ataatgcaca aattatgaat aatctgggct acagcctgct ttccgattcc
     6841 aaacgtttgg acgagggttt cgccctgctt cagacggcat accaaatcaa cccggacgat
     6901 accgccgtta acgacagcat aggctgggcg tattacctga aaggcgacgc ggaaagcgcg
     6961 ctgccgtatc tgcggtattc gtttgaaaac gaccccgagc ccgaagttgc cgcccatttg
     7021 ggcgaagtgt tgtgggcatt gggcgaacgc gatcaggcgg ttgacgtatg gacgcaggcg
     7081 gcacacctta cgggagacaa gaaaatatgg cgggagacgc tcaaacgcta cggaatcgcc
     7141 ttgcccgagc cttcccgaaa accccggaaa taatgcaggc ccatcctttc agacggcata
     7201 aggtttgccg ggaagccggg gcattcgggc aaacggcacg cagttcgcac gcgttttgca
     7261 cggcacgccg cacccgtcgg ccggcaggat gacacccgtt aaggaaactc tgatgaaaca
     7321 caccgtatcc gcatcggtca tcctgctttt gaccgcttgc gcgcaattac ctcaaaataa
     7381 cgaaaacctg tggcagccgt ccgaacacat cagcagtttt gcagcggaag ggcggttggc
     7441 agtcaaagcg gaagggaaag gttcgtatgc aaatttcgat tggacatacc aaccgcccgt
     7501 ggaaaccatc aatatcaaca cccctttggg cggcacgctc ggacagttgt gtcaagacag
     7561 ggacggcgca ttggcagtgg acggcaaagg aaatgtctat caggcagagg gtacggaaga
     7621 cttgagcagg cagctggtcg gtttcaaact gccaatccaa tatctgcata tctgggcgga
     7681 aggcaggcgt gtggcgggcg cgccttaccg catccgttca gacggcatat tggaacaata
     7741 cggttggaca atcggcagaa ccgccgacag tggggggcaa gtccgaacgt tgcaactgaa
     7801 taacggaaat ttgaacatca ggctggtttt caccgaaatt ggaatgccgt ctgaaaccga
     7861 aaccccggaa cgctgtgcgg cgcgcacgag ataaggcgga cagatgaata ttgcggacgg
     7921 acggcaggcg tttcccgcac ctgcaaaact gaatctcgat ttgaggatta ccggcaggcg
     7981 ggaagacggt tatcacaata tcgaaagcat attctgcctg atagatttgc aggataccgt
     8041 atatttgaaa ccgagggacg acggcaaaat catcctgcac aatcctgtcg gcggaatacc
     8101 gcaggaagcc gatttgagct accgtgccgc atcgttgctg caaaaatatg cgcgcaacct
     8161 tgccggcgtg gaaatatggc tggacaaaaa aatcccgacc ggcgccggct tgggaggggg
     8221 gagttccgat gccgcaacgg ttttgctggt gttgaaccgt tggtggcagt gtggtttgac
     8281 gcagtggcag ctcattgatt tgggtgcggc tttgggtgcg gacgtaccgt tttttatttt
     8341 cggaaaaaat gcttttgcaa gcgggatagg tgaaaaactg ataggaatgg atattccgaa
     8401 acagtggtat gttattgtca aacctcccgt ccacgtttcc accgcaaaaa ttttcacata
     8461 cgaaggcttg acacgggatt ctgcctcaag cataatgccg actttccaaa acctgcaacc
     8521 gttcagaaac gatatgcagg cagtggtatt taaagaatac cctgaagttt ggaaagctta
     8581 ttccgagttg tcgaaatacg gttctgcaat gatgaccggc tcaggagcct gtatatttgc
     8641 agcatttcaa gctaggaata gcgcatacaa tatataccga caagtttcag gtttgtacga
     8701 ggcatatttg gcagagggtc tttcaaaaca tcctttgttg tccgtataaa cattgttggg
     8761 gagtcgtcaa gcggttaaga cactggattt tgattccagc atgcgaaggt tcgaatcctt
     8821 cctccccagc caagccaaac aagttgggga gtcgtcaagc ggttaagaca ctggattttg
     8881 attccagcat gcgaaggttc gaatccttcc tccccagcca gacaaaagcg tgtaagcctg
     8941 cttacacgct ttttatttca tagaaataaa aatattgaaa cgcctttgtt tgtgtcggat
     9001 gttgcaggta taatgccggg cttggtacaa gcggagggaa gcattgtgtt ttctgagcgg
     9061 aagttaaaca taaaatcagg tgagaatatg gctgcgtacg acagtttgat ggtatttaca
     9121 ggcaatgcca atcccgaatt ggcacaacgt gttgtcaggc atttggacat ttctttgggc
     9181 aatgcttccg tatccaagtt ttcagacggc gaagttgccg tcgaactgtt ggaaaacgta
     9241 cgcgggcgcg acgttttcat ccttcagccg acctgtgcgc cgaccaatga caacctgatg
     9301 gaaatcctga cgatggcgga tgcactgaag cgtgcttcgg caggtcgtat taccacagcc
     9361 attccgtatt tcggctatgc gcgccaagac cgccgtccgc gttccgtccg cgttccgatt
     9421 tctgccaaac tggtggcaaa tatgctgtat tcggcaggga tcgaccgtgt tttgactgtc
     9481 gatttgcatg ccgaccagat tcaaggtttc ttcgatattc cggtggacaa tatttatgcc
     9541 actccgattt tgttgaacga catcaagcag cagcggattg aaaatctgac cgtcgtcagc
     9601 ccggacatcg gcggtgtcgt ccgcgcccgc gccgttgcaa aatccctgaa tgccgacttg
     9661 gcaatcatcg acaaacgccg cccgaaagcc aatgtggcgg aagtcatgaa catcatcggc
     9721 gatattcaag gcaaaacctg tctgattgtg gacgatatga ttgacactgc aaatacgctg
     9781 tgcaaagccg ccgtcgcctt gaaagagcgc ggtgcagaac gtgtcttggc ttatgccagc
     9841 cacgccgtat tctccggaga ggcggtcagc cgtattgcct catccgaaat cgaccaggtg
     9901 gtcgtaaccg ataccattcc tttgtctgaa gcggctaaaa actgcgaccg tatccgtcag
     9961 gtaacgattg caggtttgtt ggccgaaacc gtccgccgca ttagcaatga agaatccgtc
    10021 tcatatcttt tcaatgaaga agtgatgaca ggcagcatgt tgctgccgta accccgatgc
    10081 cgtcttaagc tggtcgcggc cgatgacggc gattttacct aacttggagt atttaacatg
    10141 acttatgaaa ttcaagcctc tgttcgtgaa gcacaaggca ctggtgcgag ccgccgcctg
    10201 cgtcgcgaag gccaaatccc cggtattctg tacggtgaag gtcaagagcc cgttgcgatt
    10261 gccgtagatc acaaaaccgt attctacgca ctggaaaaag aatctttcca tactgcgttg
    10321 attaaattgt ctctgaacgg cgaaaccaaa gacgttatcg tccgcgattt ccaaatgcac
    10381 ccgttccgcc gcgaagtcca acacatcgac ttccaagctg tgaaagccga tcaacttgta
    10441 cgcatccgcg ttcccctgca cattgttaac gctgaaaatt ctcaagccgt taaattgcaa
    10501 ggcggtcgcg tatctctgct gaacacggct gttgaagtgg ttgcgttgcc tgccaatatc
    10561 cctgctttct tggatttgga ttgtgccgaa gtggttgccg gcgacatttt gcacttgtca
    10621 gacatcaaac tgcctgaagg tgtagaaagc gtttccctga aacgtaacga aaatctggct
    10681 gttgctaccg ttaccggtaa aaagcgctaa cttacttcca tcagcagggc ggcgcgtatg
    10741 caatacgtac cgccctgttg ttttatgccg tctgaaccgt gtttcagacg gcatttcttt
    10801 atttgttgga aaagcgggat atttgaaacg gcagattact gccctgtcag acgcgtccaa
    10861 agcctttgcc accggcttct tttttctaca ttttccagtg cgacgatttc tttttcggca
    10921 atggtatgtc cgttttgcct gattttgatt tttcctaaaa tctgcccttt ttttaccggg
    10981 gcgggaatcg gctgtatggt ttccaaaatc tgttctgcca ttttcgcttc tttatgtggc
    11041 agagtgatgt aggcttcttt gaggaagcct gcgcggacgg tttttttgct gcctccggaa
    11101 atttggattt gggcaacggt tttgcctttc ggatatattt tgggcgtatc gaaggcctgc
    11161 aatgcccggt tcagcagctt gctgttgtcc gatgcgcggg tttccgccga ttccgaacct
    11221 agtgtgatga caaggatgtg cctgccgttg ccggagtatg acacggcaag gttgtagccg
    11281 ccgctttctg tgtgcccggc tttcaggccg tttacattgt tgtccctata taaaaggata
    11341 ttgcggttgt tttgttctat gttttcaaac ttgaacgatt tgatggaaaa cagcgggtaa
    11401 tattccggaa agtcgcgcat caatgcttca gacagcaggg cgaggtcttt ggcggtggaa
    11461 acctgtcctt ctctacccaa gcctgtcggg tttttgaata cggtgttctt catgcccaag
    11521 cgtcgggctt ctttgttcat ttgttgcaca aaattttcaa tcgagccgtt gcccagccgg
    11581 tcggcaaggg ttagggcggc atcgtttgcg catagggcaa tcatgccttt taagagtttg
    11641 tcggtactga ccgtatcgcc gggacgtaca aacattctgc ttccttctga agcccatgcg
    11701 gattcgggta tttttaagtt ttcttcagat tggatatttc ccgatttcat gtttttgaaa
    11761 accaaatatg cggtcatcag ttgggttagt gccgccggtt cgacaggggt attggtgttt
    11821 ttggcggata aagtctgcct gctttgaagg tcgataacga tgtgtgccgc cgtgagggtt
    11881 tcgggtgttt ggagcgtggg ggcggcgtgt accgtcggtc tgttgggcgc gggcgatgca
    11941 gccgttgcgt gagaaacgcc taagatgatg ggaagaagga cgggcaggat tttatgtgct
    12001 gtcatgaaat attctaaatt gtgtgagtgt ttcagcctgc cgattatacg cttagggtgt
    12061 ctgatcgggc ggatttttct tgctttcgcg ccgtcttggg cgtatggttt tgggttttgc
    12121 gattttaata aaccgattat cccgcattga attatgaaca cgccccttcc ttattccgat
    12181 tacctcatcc gcatcctgac ggcatctgtc tatgatgtag cggtcgaaac gcctttggaa
    12241 ccggcacgca gcctttctgt acgtttgaaa aacaacatcc ttttgaaacg cgaagatttg
    12301 cagccggttt tttcgttcaa aatacgcggc gcgtacaaca aaatgtccaa gttgccgaaa
    12361 gatgcgctcg cttgcggcgt gattgcggca agcgcgggca atcacgcaca aggcgtggcg
    12421 ttgtccgcac agcgtttggg ctgccgtgcc gttatcgtca tgccggagac tacgccgaaa
    12481 attaaagtgg atgcggttaa aagccgtggc ggcgaggtgg ttttgcgggg tgtttcatac
    12541 aacgatgcct acgattatgc gatggagctg gcggaacagg aagggctgac ctatatcgcg
    12601 ccgtttgacg atcccgatgt gattgcggga caggggacgg tggggatgga aattgtcagc
    12661 cagcatcccg atccaatccg cgccgtattc gtaccgatag ggggcggcgg tttggcggcg
    12721 ggcgtggcgg catttatcaa acaggttcgt cccgaaatta aagttatcgg cgtacagacc
    12781 aacgattcct gctgtatgaa gcagtcggtc gaagcaggcg aaatcgtcca tttgaaagat
    12841 gtcgggctgt tttcagacgg tacggcggtc aaagtcgtcg gaaacggaac cttccgcctc
    12901 tgcaaagaac ttttggatga aatcattaca gtcgataccg atgcggtttg cggcgcggtc
    12961 aaggatattt tcgatgacac gcgcagcatt accgagccgg ccggcgcgtt ggcgttggcg
    13021 ggtctgaaag cctatattgc ccgagaaggt gcggaaaacc aaaccctgat tgccgttacc
    13081 agcggtgcga atatgaattt tcaccgtttg cgccacgttt cggaacggag cgaattgggc
    13141 gagggcaacg aaggtatttt tgccgttacc atccccgaag aacgcggcag cttccttaag
    13201 tttgtcaata tattgggaaa taggaatatt accgagttca actaccgcta cggcgacgat
    13261 gaaaaagcgc atatctttgt cggacttcaa gcggcaggcc cgcaggattt ggcggttatc
    13321 ggcagccggt tggatgaggc gggattgccc aatgtcgatt tgaccaataa tgagattgcc
    13381 aaaatccata tccgctatat ggtcggaggg cggacggaca aagtagggca cgagcgtctg
    13441 gtcagttttg agtttccgga gcgtccgggc gcgttggcgc gctttttgaa ccatatgcag
    13501 ggcggttgga acattacgct cttccattac cgcaaccacg gtgcggatta cgggcggatt
    13561 ttggtcggta tcgacgtgcc gccgcacgat gccgccgcat ttgacggttt cttggaaagt
    13621 ctgggataca gctatcacga ggaaacgcaa aatgccgcgt acaagctgtt tcttgcctga
    13681 cgtttgacac gcaatgccgt ctgaaagcct ttcagacggc attgcgcttt cagggttaaa
    13741 tcgaatattc aatcaactcg ttttgagaaa aaacataaac ctgtttcgga attaagttta
    13801 attttttgcc ttcggcgatt gggtagcgcg cggcatcgct gccagccagt gtgatatgca
    13861 cgtcttgttt gccgtgtttc accaaaacgt gcgtcaatgc gccgacggcg tggatttttt
    13921 cgatttcggc acggatcatc ggggtttcgt gttcggcggc gatctgccat tcgtgcgggc
    13981 ggatatagcc ggtggcggtt tgctcctgcc atttgtattg cgcgtccaat ttccacgcga
    14041 agccgttgta atgccagatc cctttttcga tgcgtccttc aaatgcgtcg gtttcgccga
    14101 ggaactcggt aacgaaggcg ttttcgggtt tgcggtaaat ggcttcggca ctgccggttt
    14161 gttcgatttt gccatggttc atcacgacga tttcgtcgga aacttcgagg gcttcttctt
    14221 ggtcgtgagt caccagaatg ctggttacgc cgaggttgtg atgaatgtcg cgcagccagg
    14281 tgcgtaattc tttgcgcact ttggcatcca gcgcgccgaa gggttcgtcc aaaagcagga
    14341 gtttcggttc gaccgcaagc gcgcgggcga gggcgatgcg ctggcgttgc ccgccggaga
    14401 gttggtgcgg ataggatttg gcgaggtggg agagttgtac gagtttgagc aattcttcga
    14461 ctttggcgcg gatttgtcct ttggacgggc gttcgggctt gggcaatacg gtcaaaccga
    14521 aagcgacgtt gtcaaacaca ttcatgtggc ggaagagggc gtagtgttgg aacacgaaac
    14581 cgactttgcg ctcgcgcaca tgcttggcgg ttacgtcttg gccgtcaaag agaatttggc
    14641 cgccgtcggc gttttccagt ccggcgataa tgcgtaaaag tgtggttttg ccgcagccgg
    14701 agggaccgag cagggaaacg agtttgccgg cggggacgtt gaggttgatg tttttcagcg
    14761 cgtgaaaatt gccgaagcgt tttttgttta agttttgaat ggtaatactc atactgcgtt
    14821 cctttcggcg gcggcgagtt ttttgtctt
//
LOCUS       DAXDXW010000063        14699 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXDXW010000063 DAXDXW010000000
VERSION     DAXDXW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14699
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.064"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(323..4492)
                     /locus_tag="ACFB64_001609"
     CDS             complement(323..4492)
                     /locus_tag="ACFB64_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC1209940.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPAPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWIAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITDISAGDIAIVTKPTPPKEERPPQGLPDSIDLPA
                     TVYLDRFETGKISMGKTFDKQTVYLERLNAAYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGFEGETIHSTARLSGSLKDVRAELTIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGTGTARTDGSLAIASDPAN
                     EQRKLVFDTVNISAGEGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQFPAGNING
                     SIHLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIVKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGTARN
                     LHIGKAADIRSLDFTLKGSPDTSRPMRADIKGGRLSLSGGAAVVDTAGLTLEGTGAQH
                     RIRTHAAMTLDGKPFKLDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AEHVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGHNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIGNAFGGNMANTPLGGRITASLPDLGALKPFLPAAAQNITGSLNASAQ
                     IGGRVSSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFWHEGTAELSGTVSMENSVPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAASLPVNMNLTLDLN
                     DGIRFSGYGADVTIGGKLTLTAQPGGNVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYGISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRLFGSD
                     KKDSAGNGKGK"
     gene            complement(4547..6394)
                     /locus_tag="ACFB64_001610"
     CDS             complement(4547..6394)
                     /locus_tag="ACFB64_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC1209941.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     SKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQTVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYQGNYWTSNVSYNRSTTQNIEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6766..7995
                     /gene="sstT"
                     /locus_tag="ACFB64_001611"
     CDS             6766..7995
                     /gene="sstT"
                     /locus_tag="ACFB64_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC1209942.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8146..9426
                     /locus_tag="ACFB64_001612"
     CDS             8146..9426
                     /locus_tag="ACFB64_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC1209943.1"
                     /translation="MFKRSVIAMACIFPLSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     NAGEGVLPKEKKDEEAAGGAPQADTQDATAGEGSQDMAAVSAENTGNGGAATTDNPKN
                     EDAGAQNDMPQNAAESANQTGNNQPAGSSDSAPASNPAPANGGSDFGRTNVGNSVVID
                     GPSQNITLTHCKGDPCNGDNLLDEEAPPKSEFESLSDEEKIKKYKKDGEKFTGLVAIK
                     VENNGLNKYTIIYQAQPTRSARSRRSLPAEIPLIPVNQADTLIVDGEAVSLTGHSGNI
                     FAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLAGTAVYNGEVLHFHMENGRPYP
                     SGGRFAAKVDFGSKSVDGIIDSSDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGRF
                     YGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKDRD"
     gene            9426..9629
                     /locus_tag="ACFB64_001613"
     CDS             9426..9629
                     /locus_tag="ACFB64_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC1209944.1"
                     /translation="MLMPPEAQVFITAGRRVQIIKQAPEKGGIPKSGTEIAARIGGIP
                     QPAGETVGSSRNVGYFRPPFSGC"
     gene            9689..9937
                     /locus_tag="ACFB64_001614"
     CDS             9689..9937
                     /locus_tag="ACFB64_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209945.1"
                     /translation="MFQHSAGSNRQQQGDNALNPALRGGRVRAGLGKGIAFPNRGGAT
                     GCALCAERVCGNGMPSEAGAFRRHCDVWGAGPVQIRIS"
     gene            complement(9930..10145)
                     /locus_tag="ACFB64_001615"
     CDS             complement(9930..10145)
                     /locus_tag="ACFB64_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209946.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDNGFIN"
     gene            complement(10138..10353)
                     /locus_tag="ACFB64_001616"
     CDS             complement(10138..10353)
                     /locus_tag="ACFB64_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC1209947.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10556..11899)
                     /gene="argG"
                     /locus_tag="ACFB64_001617"
     CDS             complement(10556..11899)
                     /gene="argG"
                     /locus_tag="ACFB64_001617"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC1209948.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            12024..12824
                     /locus_tag="ACFB64_001618"
     CDS             12024..12824
                     /locus_tag="ACFB64_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC1209949.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDRTRQTAAAALDLPVVRHPEAAKFIEG
                     ITRKRGKPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYAGRFNQTERGSRSVYVFEQPESRITPIIEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            12864..13850
                     /locus_tag="ACFB64_001619"
     CDS             12864..13850
                     /locus_tag="ACFB64_001619"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC1209950.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYRDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGKIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVSEALQAVR"
     gene            14511..14699
                     /locus_tag="ACFB64_001620"
                     /pseudo
     CDS             14511..14699
                     /locus_tag="ACFB64_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         3089 a         3756 c         4439 g         3415 t
ORIGIN      
        1 tttcttaacc atcccttcca atagccgtgc gaaggttttt ttatatcagt gggaaatgca
       61 atattctatt gtttttattg tagaatttta aaaacagatt gttgtgttcc gcgtttttgc
      121 cggtttggaa gcggtggggc gcatcagcct tttataaagg catatcggaa gcctgtataa
      181 ggtttttgaa catatcgatc ctgttccctt gcaggcggac ggcagcggcg gatggcgaaa
      241 ggcgggtttt caaatgtccg gtttggcggc gcgtctttca gacggcatgg cggcggagcg
      301 tgccgcgccg tctgaaaacc gtttatttcc ctttgccgtt tccggcggag tcttttttgt
      361 ccgaaccgaa gaggcggtcg aaacgtatgg tgtatgtcag ctcgccgccc gacgaacggc
      421 tgccgatacg ggcaaccgcc tgtatggcgc gggtcagccg gtaaatcagt ttgacggact
      481 gttccgcgct ggagatgccg tattcgtagc cgatgtagag tttgccggtc agttgtttgc
      541 cgacggtcag cacctgttcg gcggggttga gttcgccggt ttgcgcgttg cggctgcgct
      601 tgctggtaaa gcccaaatca tccaccagcc cgatgcggtc gttgatttgc ccggcaagca
      661 gcgcgcctgc ggctgcggac agggcggcat tgtcgccgct gctgccgctg ccggcgcggt
      721 tgaggatgag ccaggagagc ttgtcttttt cactcatcgg ttcgtttgcc gtcagcgtaa
      781 tgcgcgggct gttgaggctg cccaatattt ccacgcccgc accgacgggg gaaaggcggc
      841 gttcggcgcg gatgttgagg ttggggtcgt tgagcgggcc gacaaaggag actgtgcctt
      901 tggtaatgtc taaatcctgc ccgtatgctt tgtaacgccc tttgatgacg cggaccgtgc
      961 ccaccccacg cacatttccg cccggttgcg cggtcagggt cagtttgccg cctatggtaa
     1021 cgtccgcgcc gtagccggag aagcggatgc cgtcattgag gtctaaagtc aggttcatat
     1081 tgacggggag cgatgccgcc gcctctttct tgacttcgcc caatacgacg acatcgtcgc
     1141 cgacggacgg catcgaggat ttttgcgaac cgaacagccc ctgatcagtt ttaatcatac
     1201 cggtaacgga tatgcctttt tgcggcgaat agcgcaggcg ggtgttgccg gaaaccgtca
     1261 ggcggcggtt ggggcgggac aggatgcggt atttgtcgaa caccgcgccg atatcgacat
     1321 cgggcacgct gttttccatg ctgaccgtgc cggagagttc cgccgtccct tcgtgccaga
     1381 atttcaggct gtcgattacc catttcctgc ctgcaatatg cgaacgcagc gagccgttgt
     1441 ccaagatgat gccttgggtt tggttgcggt aatagagctt gtcgccgttg atactgccgc
     1501 ccaagtgcgg gtcggcgatg ctgccgccga gggttacggc ggcattcagg ctgcctttga
     1561 cggtttgtcc gaccggtagg aagttgcgga atacttcggc atcggcaacg gtcaggttga
     1621 gcctgccgcc caaaggtgcg gtatcgaagg agcggctttg cccgacggtg atattgccgt
     1681 tgattttccc gtagttgctg ctaccgttga cggcggcatt gacggacgga gagcttaccc
     1741 gtccgccgat ttgcgcggag gcattcaggc tgccggtaat gttttgcgcg gcggcgggca
     1801 gaaagggctt caatgcgccc aagtcgggaa gggaggctgt aatcctgccg ccgagcggtg
     1861 tatttgccat attgccgccg aaggcgttgc cgatgcccaa atcggcgtta atccgtccga
     1921 aacgcgcgcc gccgtcaagc aggattccga tgcggtcgtt ttgaaagcgc gttttcaggg
     1981 aaaatgcgtt caaacccaaa gcctgcccgc cgggcaatac ggcatcgccg ctttgccggc
     2041 tgatattgag gtagccgcgc gcgttgtgcc cgtaggcgac atcccagtcg ccgtttaaaa
     2101 ccagattgtg ttcgaagggc ggtttgaaga aattgtgcaa ctcggcgatg tgcaggccgc
     2161 gtgcgccgcc ttttgccgat atgccggttt tcctgtccca agaaaagtgt tgcaggttga
     2221 ggctgccgcc cattgcctgc caatttgccg cacttgccgc cacgtgttcc gcaccggctt
     2281 cgagcgtcat acggttttgc agcttgaggt tgaatgcgcc gccgatgtcg aggatgccga
     2341 tgctgccttt ccatcgggta agttccctgt tgatgccgcc tgaagcgtcc aaatcgagtt
     2401 tgaacggttt gccgtccagc gtcatggcgg cgtgtgtgcg gatgcggtgc tgcgcgcccg
     2461 taccttccag cgtcaggccg gcggtatcga caaccgccgc gccgcccgac agggaaaggc
     2521 ggccgccctt gatatcggcg cgcatcgggc ggcttgtgtc gggcgagcct ttgagggtaa
     2581 aatcgagcga acggatgtct gccgctttgc cgatgtgtaa gttgcgcgcc gtgccggaaa
     2641 ggtcggtttc aaaggttcgg atgccgccgt ccaaatcgcc ggaaaggtgt ccgcgtacat
     2701 ttaaagaccc cgcgagtccg aaaccgaaac gggataaatc gggtgcggtg atattgaggt
     2761 taagccggtc gccttttttg ccgaagccgc cgtctgtttt gacgatgttc cgccccaacc
     2821 gcaaatcgac ggcggcacgc ggaaggtggc gggactcgta aacaatgtcg gcactgccgg
     2881 caatcggcac gccgttgaac gtaccgggca aaaaacgcat tttgcccgta aatttctctt
     2941 ttgccagttc accggcaaga tgaatcgaac cgttgatatt gcctgccgga aattgcggat
     3001 cgatgcgtga agggtcgaat gcgcgggaac ggatgtccag cttgagcagg cggtctttaa
     3061 acagctcgag atagccttgc gcggtcaggc tgccttcccc ggcggagatg ttgacggtgt
     3121 cgaacaccag tttccgctgt tcgtttgcgg ggtcgcttgc gatggcgagg ctgccgtccg
     3181 tgcgtgccgt gccggtgccg agttgccaag agattttggg cgaggcggtc gtgccgccga
     3241 tgccgatgct gccgtccaac ctgcctttga acgcggtttg cagcacgtct tccgcgccga
     3301 cggagttgat gcctatattt aaatcaagga tgtctttttc ggtgtcgatt ttgcccgaca
     3361 gcctgatgcc gccccgtccg agcagggcgg cggacgtatt gccgatatgc accgtgccgt
     3421 cctgccggat gacaaagccg cccaaaacct gacggacggg gatgccgttg cggtcggcaa
     3481 agccggcttt ggtgttttcc aaatcgagcg agccttccag cgcgatgccg tctgaaaacg
     3541 acgggatggc ggtcaggtcg aaattcagcc cggcatcggg cagggaaggc acgaaggcgg
     3601 acggattgat gttgaatcct ttgaccagta cttcttccaa tgttttatcc aatgattcgg
     3661 caaacgggtg gatgacggat tttcccgaga ggcggatatt gccgccgtcg atcgtcagtt
     3721 cggcgcgcac atccttcagg ctgccgctca gccgcgccgt actgtgtatg gtttcgcctt
     3781 cgaatccgcc tttggtgtaa atggcggtat cgagggcaaa cggttttttc aagccgaccg
     3841 aggctgaccc cgacgaactg ctccacggcg tgtcggcggc cttcaggtcg aggcggtgcc
     3901 ctttacggtc gtaacggtat gccgcgttga ggcgttcgag atagacagtt tgtttgtcaa
     3961 aggttttgcc catgctgatt ttgcccgtct cgaagcggtc gagatagacg gtggcgggca
     4021 ggtctatgct gtcgggcagg ccttgaggcg ggcgttcttc tttaggcgga gtcggtttgg
     4081 ttacgatggc gatgtcgccg gcggagatgt cggtgatgtg caggctgcgg cgcatcagtt
     4141 cggacggttt ccacgcgaag cggaagcggc tgattttaag gtctgccccc tcggtttcta
     4201 tcgaccagtt gtcgccgtcg aagccgtcga gcagtgtgcc tttgaggttt tgggaggaaa
     4261 tgtttacgcc gaaccaggac gggatttggt acagcccgaa gcgcaaacct gcttccgtac
     4321 cggcgatcca gccgaggaaa catactgcca aaatcaggac agacagcagt gccgccgaca
     4381 gcttcagcag cgggcggcgt ttttttgccg gcggggcggg gcggtgttca gacggcattt
     4441 tgcgcgtgcc gttttcggtc ggatcggtat ctgtcggtgc ggtatcggtc ataatgtctg
     4501 tttcaaaatg gtttgctgca atgccgtctg aagcggccgt atcggtttag aaacgcgttc
     4561 ccaagctgat gtgccagcgg atttttttgt cgctgtgtcc gtaggcgatg tcgaaggaaa
     4621 acggcgcgag cgggctgaac cagcgcacgc ccagtcccga accgtgtttc agcttcatac
     4681 gtttgaaatt ggcggcggca tcgcccatat cgtggaatac cgcgccggac agggtgcgcg
     4741 taaacggcag ttggtattcc aagctgccca ccaagagggc gcgttcgggc aggaccgatc
     4801 cgttcggacc ggcaagcccg atgctgtcaa gttcgtaacc gcgcacggaa gacgcgccgc
     4861 cgctgcggaa catcagcccc gaggggacat cggcattgtc gcgtgcaacg gtgtaacccg
     4921 cttgtccgcg tatgatgaac gtgccgagtt ttttgttttc gggcgtgaag aaataacctg
     4981 cgcgggcaga ggtgcggatt agcgcggtgg aggacaggaa tgtgcccaaa gtcgtcccga
     5041 ttttgccgtc gaggtaatgg ccgttttcgg ggtgcagcac gttgttgagc agctggcgtt
     5101 tccaagaggc ggtcagcatc gtggcgtggc tgttgcccaa atcgacatcc gagccgggga
     5161 ttttccggcc ttctgcgaga aattccgccc ccagcctggc atcgatgccc gcgcggtcgc
     5221 gcacatacca gatgccgccg gagaaggcgc gtttttcaat gttttgggtg gtcgaacggt
     5281 tgtaggaaac gttgcttgtc cagtagttgc cctgatagtt gcgcggctgg ctgatgccgg
     5341 cggcaagcgt ggtttcgtat ttgtccatat cccagacgac cgagccgata tagcctttgt
     5401 tgaagaggtt gtaatagtcg taggcgattt tgccgcccaa accgtattcc gaatcgaggc
     5461 ggatgccggt ttcgagtttg tggcgtttga cctcggttac gctgactttg acggggacgc
     5521 ggtcgccttg gaggcggtcg aagtcggctt gtacggacgc gccggaataa tgcccgtttt
     5581 gttcgagcgc ctgttggaag tcgagcagca ggtcgaggtc gtagggcgtg cccggttgga
     5641 agcgcgccag gccggagacg gtttgttcgg ggtaacgctg tgtgccggtg atttcaaagt
     5701 cgccgaaggc gatggggcgg ccgctgtcca cgacgacgtt caaatcggcg gtggcggtat
     5761 cggggttgac ggccgcccgg gtgttgccga gcttggcaag cgggtagcct ttgcgcgtta
     5821 ccgcgccgag gacggaagtt ttgctgtttt cccaactgtc ctgatcgaaa tcgctgccta
     5881 ccggctgctg ccagttttcc agcgcgttgc ggtagtattc ggcgaggttg ccgtctgaaa
     5941 ggatgtcgcc gaggatggcg acgccgacgt tggcgatttt ggtgcgcggg cccggtgtga
     6001 tgtgcaccgt ataagctccg tctttttccg tcaggctgac cttgctgctg aaatagcctt
     6061 tgctgcggag catggttttg acgttgtccg gtgcttcttc ggcaaggaat cccgtctgtt
     6121 ccttatccaa aacctcttcc tgctgctgcg tgatgagcgg caggtgttct tcgaccatat
     6181 ctttgatttc actgtcctgc gtgtcgatgc ggacggggaa tttgggtttt aatttgactg
     6241 attcggtgtc ggggcttttg cttttgaaca atgcgaaacc cgccgccttg ttttcggaaa
     6301 ggtcggcggc aggcgcgtat gcgtgcggaa agaaaaataa agccggcagg agcagggcgg
     6361 tcggtttgat catcatggta cgggtgtcgt gcatcggtgt ggcggatttc gattctaaca
     6421 ctgttcggac ggggcgggac gcttcagacg gcatactgcc gacggtattt cggaggttgc
     6481 ggcccgccgg aaatgtatct gcttgtttta aggcattttg tttccaatcc gcatatggaa
     6541 aacaggctgc gtttcggaat agtgtcgaaa aataggcagg attaaataaa tttattcttg
     6601 ttcaataaat taaaccgttg cgccgaatga taaaacaaag cctgtccctg catagggcaa
     6661 atcaacgcac ctcaaaacac agcttgaaaa ataaaaaaaa cacagagaat gagttttctt
     6721 ttaaaataca acatttttta acatatcaca acataaggaa gaattatggc gttcggcaaa
     6781 tccttatttc atgcgatcgg ccgcgtcagc ctggtcaggc agattgccgc cggtttggcg
     6841 ttgggcatcg taatcggttc ggtttcaccg caactgggct tggcggcagg cttgttcggc
     6901 agcctgtttg tcggcgcgct caaagcggtc gcgccggttt tggtatttat tttggtggcg
     6961 gccacaatcg cgcagcacca aaaaggcaac aaggcgcata tcaggccgat tatcgtcctt
     7021 tacctcatcg gcacgttttc cgcagccctg accgccgtca tcgccggtat ggttttcccg
     7081 acgcacattg ttttggcggg cgcgggcgat gtgtccgccg cgccgccttc cggcattgtg
     7141 gaagtgttga aatcgctgct gatgaacctg gtcgccaacc cgattaacgc gattgccaat
     7201 gccaactata tcggcatttt ggcttgggct ttggttttgg gcgcggcgtt gcggaatcac
     7261 ggttcggacg ttacgcggca ggtcgttgcc gatttggcgg aagcggtttc caccgtcgtg
     7321 aaatggatta tccgttttgc ccctttgggc attttcgggc tggtgtcgtc cacaatcgcg
     7381 gagacgggtt tcggcgcgct ggcgggttat gcgaagctgc ttgcggtgct gttgggctgt
     7441 atggcgttta tcgcgctggc ggtcaatccc gccatcgtgt ggtggaaaat ccgccgcaac
     7501 ccttatccgc tggtgtttac ctgcctgcgc gaaagcggcg tgtatgcctt ctttacccgt
     7561 tcttccgccg ccaatatccc cgtgaatatg gctttggcga aaaagctggg gctgcacgaa
     7621 gacacttatt cgatttccat cccgttgggg gcgaccgtca atatgggcgg cgcggcgatt
     7681 acgattaccg ttttggctat ggcggcggcg cacacccaag gcatacaggt tgattttgcc
     7741 accgcgctgc tgttgagcct ggtggcgacg gtcagcgcgt gcggcgcgtc cggcgtggcc
     7801 ggcggttcgc tgctgctgat tccgctggcg tgcagcctgt tcggcatcag caacgatgtc
     7861 gccatgcagg tggtcgccgt cggcttcatc atcggcgtga ttcaggattc ggcggaaacg
     7921 gcgttgaatt cttcaaccga cgttttgttt accgctgccg ccgatttggg ccgacagaga
     7981 aaccgtgcgg aataaaccaa gctatgccgt ctgaagcccg tttggcgttc agacggcata
     8041 tttttacaat tttatgggca tatggcggaa acaataccgg ctgccttgaa tatgtttcaa
     8101 cacacaggtc gatacataaa gcgtcgccct atgaaggaga tgatgatgtt taaacgcagt
     8161 gtgattgcaa tggcttgtat ttttcccctt tcagcctgtg ggggcggcgg tggcggatcg
     8221 cccgatgtca agtcggcgga cacgccgtca aaaccggccg cccccgttgt tgctgaaaat
     8281 gccggggaag gggtgctgcc gaaagaaaag aaagatgagg aggcagcggg cggtgcgccg
     8341 caagccgata cgcaggacgc aaccgccgga gaaggcagcc aagatatggc ggcagtttcg
     8401 gcagaaaata caggcaatgg cggtgcggca acaacggaca accccaaaaa tgaagacgcg
     8461 ggggcgcaaa atgatatgcc gcaaaatgcc gccgaatccg caaatcaaac agggaacaac
     8521 caacccgccg gttcttcaga ttccgccccc gcgtcaaacc ctgcccctgc gaatggcggt
     8581 agcgattttg gaaggacgaa cgtgggcaat tctgttgtga ttgacggacc gtcgcaaaat
     8641 ataacgttga cccactgtaa aggcgatcct tgtaatggtg ataatttatt ggatgaagaa
     8701 gcaccgccaa aatcagaatt tgaatcgtta agtgatgaag agaaaattaa gaaatataaa
     8761 aaagatggag aaaagtttac tggtttggtt gctataaaag ttgaaaataa tgggttgaat
     8821 aaatatacca ttatttatca agctcagcct actcgttctg cacggtcgag gaggtcgctt
     8881 ccggccgaga ttccgctgat tcccgtcaat caggccgata cgctgattgt ggatggggaa
     8941 gcggtcagcc tgacggggca ttccggcaat atcttcgcgc ccgaagggaa ttaccggtat
     9001 ctgacttacg gggcggaaaa attgcccggc ggatcgtatg ccctccgtgt gcaaggcgaa
     9061 ccggcaaaag gcgaaatgct tgcgggcacg gccgtgtaca acggcgaagt gctgcatttc
     9121 catatggaaa acggccgtcc gtacccgtcc ggaggcaggt ttgccgcaaa agtcgatttc
     9181 ggcagcaaat ctgtggacgg cattatcgac agcagcgatg atttgcatat gggtacgcaa
     9241 aaattcaaag ccgccatcga tggaaacggc tttaagggga cttggacgga aaatggcggc
     9301 ggggatgttt ccggaaggtt ttacggcccg gccggcgagg aagtggcggg aaaatacagc
     9361 tatcgcccga cagatgctga aaagggcgga ttcggcgtgt ttgccggcaa aaaagatcgg
     9421 gattgatgtt gatgccgccc gaagcgcagg tattcattac ggcgggacga cgcgttcaaa
     9481 tcatcaaaca agcccccgaa aaaggcggca tcccaaaatc cggcactgaa attgccgcgc
     9541 gtatcggcgg cattccgcag cctgccgggg aaaccgtcgg cagcagccgg aatgtcgggt
     9601 atttccgccc gcctttttcg ggctgttgaa atatgaaaat aaagcatagg ccgtctgaat
     9661 gttcagacgg cctgtttttt taacggctgt gtttcaacac tccgccggtt ccaaccggca
     9721 gcagcaggga gataatgcct tgaaccccgc tcttcggggc ggcagggttc gggccggttt
     9781 ggggaaaggc atcgcctttc cgaatcgggg cggtgcaacg gggtgcgcct tatgtgccga
     9841 aagggtttgc ggcaacggta tgccgtctga agcgggtgcg ttcagacggc attgtgatgt
     9901 ttggggcgcg ggtccggttc aaatccgcat cagttgataa acccgttgtc tttcattttc
     9961 ttttcggaaa aatactggcc gcataccagc agggcggcga ggatgatgac aaacagccct
    10021 gccatagtca gtgtgatttt gaagaacaat tcggcttcga gaaaagaaaa ccaaagttgc
    10081 acaatcagca gcagcaccca gccggcaaac agtccgagac aggcataaac gccgagctta
    10141 aacacggtcc cgctccaaaa tgacgacgta ggcttcgcgt ttgaaaaaca gcagcatacg
    10201 gcgcaaatct ttttccatcg ttacgacccg ccagccttcg ggggtttgtt tgttgaggaa
    10261 cgcgctgaaa ttgacggggt tgacctttgc cgcgccgaaa aacaggctgg acaacaggct
    10321 ttcctgataa atgacgactt tgtattcttt catcgtattt ccttttcggt tgaaaccccg
    10381 ccctttgggg cggtaggatc agactttatt tgggagggaa ccccttccaa atcaggacga
    10441 tacataggtc ggtgctttat gtatcgtcct gtgtgttgaa acataggatt cctttaatca
    10501 agggattgcc ttttcaggca atcccttaaa ttgcagcgat gcaatgcggc gaaatttatt
    10561 gcttattgcc caactgcggc aataccgagc cttcgcccag cgagagcaaa ccgctttgcg
    10621 aataaatacc cagtttggcg cgggtgtcgg tgatgtcgag gttgcgcatc gtgagctgtc
    10681 cgatgcggtc gagcggtgtg aacgccgcac cttcgacttt ttccatactc aggcgttcgg
    10741 gttggtaggt caggttgggc gattcggtgt tcagaatcga gtagtcgttg ccgcgccgca
    10801 gttcgagggt aacttcgccg gtaacggctt tggcgaccca gcgttgggcg gtttcgcgca
    10861 acatcaaggc ttggctgtcg aaccagcggc cttggtagag caaacgtccg aggcgcaggc
    10921 cgttgatgcg gtattgttcg atggtgtctt cgttgtggat gccggtaacc aagcgttcgt
    10981 aggcgatgtg gaacaatgcc atgcccgggg cttcgtagat gccgcgcgat ttggcttcga
    11041 tgatgcggtt ttcgatttgg tcgctcatac ccaagccgtg gcggccgccg atgcggttgg
    11101 cttcgaggaa gagttcgacg gggtcggcgt attctttgcc gttcaatgca accggcacgc
    11161 cttcttcaaa gcgcacgctg acttcttcgg gttcaatttc gacgttttcg tcccagaagg
    11221 caacgcccat gatgggctta acgattttga tgcccgagtt caaaaattcc aagtctttgg
    11281 cttcgtgggt cgcgccgagc atattggaat cggtggagta ggctttttca accgacattt
    11341 tgtagttgaa gccgttggca atcagaaatt cgctcatttc gtgacggccg ccgagttcgt
    11401 cgataaattg ctggtcgagc cagggtttgt agattttcag cgcgggattg gtgagcaagc
    11461 cgtagcggta gaaacgctcg atgtcgttgc ctttgtaggt gctgccgtcg ccccagatat
    11521 tcacatcgtc ttctttcatg gcggaaacga gcatggtgcc ggtcacggcg cggcccagag
    11581 gcgtggtgtt gaaataggcg atgccgccgg tggaaacgtg gaacgcgccg cattggatgg
    11641 cggcgatgcc ttcgtgtgcc agttgtgcgc ggcagtcgat caggcgggcg ttttccgcac
    11701 cgtattccat tgcttttttg ggaatggcgt tgtaatcgtc ttcgtcgggc tggccaaggt
    11761 tggcagtgta ggcataaggc agcgcgcctt tgagtttcat ccacaacagc gcggcagagg
    11821 tatcaagacc gccggagaag gcgatgccga ctttctgacc gacggggagg ttttgtaaaa
    11881 tagtatggtt ttggttcatg aaaaaatcct cgatagcttg aaaaatcaaa gccattatct
    11941 tataaataga aaatattgtc agccatcatg ccgtctgaac gggtattccg ccgtttcagg
    12001 cggtatttaa aaccgaaaac attatgaacg cgttcaacct catcatcatc ggagacgaaa
    12061 tcttacacgg cagccgccaa gacaaacatt tcgccttttt taaatccctg ctcgaatcca
    12121 aagggctgaa gctcaatcag gtgcaatatc tgcccgacga acccgatttg ctggtcaggc
    12181 aactgcgccg cagcttttca gacggcatac cgacgttcgt taccggcggc atcggcgcca
    12241 cgcccgacga ccgcacgcgc caaaccgcag cggctgcttt ggatttgccc gtcgtccgcc
    12301 atcctgaagc cgccaagttt atcgaaggca taacccggaa acgcggcaag ccgctcgatt
    12361 cgcccgaaca cgcccaacgc ctgaaaatgg cggatttccc cgagggcgca gagcttgtgc
    12421 ccaacccgtt caacaacatc gccggatttt ccatccgcga gcattatttc ttccccggct
    12481 ttcctgtgat ggcgcacccg atggcggaat gggttttgga aacttattac gccggccgct
    12541 tcaaccaaac cgaacgcggc agccgcagcg tgtatgtgtt tgagcagccc gaatcgcgca
    12601 ttacgccgat tatagagcat atcgagcaaa cttatcccgg cgtgcgttcg tacagcctgc
    12661 ccagcgtcgg ttggacgcat tcagacggca cgcaggtcaa accgcacatt gagttcggca
    12721 tcaaagccga aggagaagcc gtcaaccttt tggacgcggc gtgggcggaa gtgttgcaca
    12781 gcttggacgg attgggagca gagctgaaaa atcgggtaaa ctgaaaactg tttgcccaaa
    12841 tcggacgata tttaaggaaa accatgcaat accgaatcag aagagaaaac gaagcacccg
    12901 aagcaaaaaa tgcgggggaa accttatggg agcgcgacat tatgcgcgaa gtgctgctgt
    12961 ccgcctatcg ggacaggcgc agggagcgga tgtggaaaaa catctggcgc gccgtcagca
    13021 ccctgattct ggttgccctg attgcaggca ttttccgaaa agacgaagcc gcattgcagt
    13081 tggcgggcaa cacgccgcat accgcagtcg tcaacctgta cggcaaaatc ggcaacggcg
    13141 tagaagacca ggtcaaaaaa ctcaaagacg ggatggaggc cgcctacaaa aacccgcagg
    13201 caaaagccat cgtcatccgc gccaacagcc ccggcggttc gcccgtcgtc tccaacaccg
    13261 cttttgaaga aatacgccgt ctgaaggcgc agcaccccgg catccccgtt tatctcgtgg
    13321 cagaggatat gtgcgcgtcc ggctgctact acatcgcggc ggcggcggac aaaatctatg
    13381 ccgacccgtc cagcatcgtc ggcagcatcg gcgttatcgg cagcagtttc gacgcgaccg
    13441 gcctgatgga aaaaatcggc gtaaaacgca gggttaaaat tgcgggcagc aacaaaggca
    13501 tgggcgatcc gttttcgccc gaaacgcccg aacagtcgaa aatctgggaa gaaatgctga
    13561 ccggcataca cggcgaattt atcaaagccg tcaaaaccgg acgcggcggc cgcctcaaat
    13621 tccgacaata tcccgacgtg ttcagcggtc gcgtgtacac aggcgcggac gcgctgaaag
    13681 tcgggctggt ggacggactc ggcaatattt acagcgtggc gcgagacgtg gtcaaagctc
    13741 ccgatgtggt ggattacact ccgaaggacg atttcggcag aatcttgggc agacgcttcg
    13801 gagcagaact caaagcaagc gtttcggaag ctctgcaggc cgtccggtag cgttgtttac
    13861 agtatttcta agggaatacc agaataaaaa tatacacaaa aacactcacc tccccagtat
    13921 gctgccacta agtcagccgc gttactattt accgtgcact taaagccgca aaaggcaggt
    13981 tgtccccggg aggttttgtg catcctgata ccgatcggtt gctgttgctg ttgctgttgc
    14041 tcaaaggacg gaaggccgcc gataaacgag attacctgtt tgtcgctatt gacgattttt
    14101 atactctgcc attttgccgg acaaaaccgc agacagtgct gccaagtttc tgaccgaaca
    14161 tctggctgac ccctgcttgt acctggttga gtacgtttac tctgacaatg ataggtaata
    14221 taaagagccg tccaacatgc tttcggtgca gcttgttatg ataatgggat tggttggagg
    14281 cttgcccgat ttgcttgtcc gcagaccaac ggtaaggcgg agcgggttat ccgtaccttg
    14341 atggagatgt ggcatgagga acagtcgttt gacagtccgg aacatcagca aaaggagttg
    14401 tgctgcttta ttaatttagg cgctgtccta gataactagg gaaattcaaa ttaagttaga
    14461 attatcccta tgagaaaagc cgtctaagcc ggtataaaca aaataaactc attgggctat
    14521 ttgtcgcagg tgtaactgca agaacagcgg cagagttggt aggcgttaat aaaaatactg
    14581 cagcctatga ttttcatcgt ttacgatgac tgatttatca aaacggtccg catttggaaa
    14641 tgtttgatgg cgaagtagaa gcagatgaaa gttattttgg cggacaacgc aaaggtaaa
//
LOCUS       DAXDXW010000064        14465 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXDXW010000064 DAXDXW010000000
VERSION     DAXDXW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14465
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.065"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            828..1937
                     /gene="fic"
                     /locus_tag="ACFB64_001621"
     CDS             828..1937
                     /gene="fic"
                     /locus_tag="ACFB64_001621"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC1209951.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLASRPLCTQTAIMVCNAIKHPYETAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLVIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRCLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     GIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSF"
     gene            complement(2665..3477)
                     /gene="murI"
                     /locus_tag="ACFB64_001622"
     CDS             complement(2665..3477)
                     /gene="murI"
                     /locus_tag="ACFB64_001622"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC1209952.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLGHDVKAMVIACNTIAAVAGRKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLDTGNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            3666..4127
                     /gene="tsaE"
                     /locus_tag="ACFB64_001623"
     CDS             3666..4127
                     /gene="tsaE"
                     /locus_tag="ACFB64_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC1209953.1"
                     /translation="MSDFSPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFALHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHGGGGRKCLLTAHTERGRESLPL"
     gene            4124..5374
                     /locus_tag="ACFB64_001624"
     CDS             4124..5374
                     /locus_tag="ACFB64_001624"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC1209954.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFALPPVGGFKDRLVVDLYPHGMDADDPMMALLNGS
                     LNKTLRGSPEADPAQNTTPRPGRGKNGRRPVIMLDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            5456..6274
                     /locus_tag="ACFB64_001625"
     CDS             5456..6274
                     /locus_tag="ACFB64_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC1209955.1"
                     /translation="MKGMDKLRYQRDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSPAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPKPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLRYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDVDIFRNFSI"
     gene            complement(6320..7462)
                     /locus_tag="ACFB64_001626"
     CDS             complement(6320..7462)
                     /locus_tag="ACFB64_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFC1209956.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELGSLGCTDVQAFDGGVSCRGGL
                     EQAYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFAGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAAGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDNGRRIVQTALDNAHNA
                     GADDIVSFSVAGAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLLPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(7511..7933)
                     /locus_tag="ACFB64_001627"
     CDS             complement(7511..7933)
                     /locus_tag="ACFB64_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209957.1"
                     /translation="MNKNRKLLLAALLLTAFAAFKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAARIRLPVCVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(7926..8735)
                     /locus_tag="ACFB64_001628"
     CDS             complement(7926..8735)
                     /locus_tag="ACFB64_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC1209958.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLGSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFGEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWEGGPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLYGENSMGAYRVLP
                     LGSAGGSGANVLMEAAQWQDRSEHAGSVWMMVQTREQWRQAQEKGADVAVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHAVVRDETIGKNHE"
     gene            complement(8817..9194)
                     /gene="acpS"
                     /locus_tag="ACFB64_001629"
     CDS             complement(8817..9194)
                     /gene="acpS"
                     /locus_tag="ACFB64_001629"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC1209959.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSEWLEEQG
                     ISRVSLSMADEGDTVLAFAVAEK"
     gene            complement(9244..9972)
                     /locus_tag="ACFB64_001630"
     CDS             complement(9244..9972)
                     /locus_tag="ACFB64_001630"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC1209960.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADNEQIQAARDVGAPVVELHTGAYADAH
                     SHAEQIRQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPVAQILAIRELNIGHSLISQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(10025..10768)
                     /gene="recO"
                     /locus_tag="ACFB64_001631"
     CDS             complement(10025..10768)
                     /gene="recO"
                     /locus_tag="ACFB64_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC1209961.1"
                     /translation="MSEYRVNHEPVFMLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPASVSWYGSQELKTLHRAEWMGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMSELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCRDTGALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLLIGTLLPEGLKSRQVLEQIRQFDRNTA"
     gene            complement(10805..11932)
                     /gene="pheA"
                     /locus_tag="ACFB64_001632"
     CDS             complement(10805..11932)
                     /gene="pheA"
                     /locus_tag="ACFB64_001632"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC1209962.1"
                     /translation="MLECTANYRSGEIMSQTIDELLIPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     RLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKAIGSYPTAVL"
     gene            complement(11971..13199)
                     /locus_tag="ACFB64_001633"
                     /pseudo
     CDS             complement(11971..13199)
                     /locus_tag="ACFB64_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768606.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
     gene            complement(13315..14190)
                     /locus_tag="ACFB64_001634"
     CDS             complement(13315..14190)
                     /locus_tag="ACFB64_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC1209963.1"
                     /translation="MFYQILALIIWGSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANASGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATFA
                     AGRLSRRDAQNGNAV"
     gene            14373..14465
                     /locus_tag="ACFB64_001635"
                     /pseudo
     CDS             14373..14465
                     /locus_tag="ACFB64_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT         3340 a         4141 c         3885 g         3099 t
ORIGIN      
        1 gggtggctgt tggggttaag gtttatgcaa actaatacgg cttgctgccc tctctctaac
       61 tctctcccac ggggagagag gactatgatg cccaccggcg gcttgctatt aggaatatct
      121 gaatcgtcat tcccacgaaa gtgggaatct agaacgtcaa atctcaagaa accgttttat
      181 ccgataagtt tctgttccga caggttcgga ttcccgcctg cgcgggaatg acgaatccat
      241 ccatacggaa acctgcatcc cgtcattccc acgaacctac attccgtcat tcccacgaaa
      301 gtgggaatct agttttttga gtttcagtca tttccgataa attgccttag cattgaaggt
      361 ccggattccc gcctgcgcgg gaatgacggg atttgaggtt tctgttttta attttctgtt
      421 ttcacgggaa tgacggttta aaagttaccc gaaacctcaa aaaaaaagct gtgttttaat
      481 atagtgttga tagacgtact tggcctccat gtattcaatc gtggaaatct atatcttcgt
      541 cctcgccgaa atagtctatg cccgatatac aattttgata cacaaacttg gaaatatggg
      601 tatcgtcgcc ggagcgatag aatgcggaca gttatatata tacggttttt ttaggggagc
      661 gggcagatga agggcggatt ttgaattaac cccatgcaaa ttgacttttg cgggcggttt
      721 taccgcccat atatttacaa aagccaaatt tctaaacata tatccttgat atatacacgg
      781 cgtaaacata tactggaaac atctttaaat tttccgaaat tttaaacatg agcaactgga
      841 aacccgatat tccctataac gatttaccac ccctgccgcc aaaacaggat attgaaagca
      901 aaaccatcct gaaacgttgt atagccgccc gtgcatccct tgcccgttta aagcaggcgg
      961 cagaattgat accgaatcaa gccatgctga ttaacaccct tcctgttatg gaagcccgtg
     1021 caagttcgga aattgaaaac atcgtaacca ccacggacaa gctgtttcaa tccctgcaaa
     1081 tggatacgga acggcaagac cctgccacga aagaagccct gcaataccgc accgccctgt
     1141 ttgcaggcta tgaatcactg gcgagccgcc ctttatgcac acaaaccgcc atcatggtct
     1201 gcaacgccat caagcacccc tacgaaacgg ccatccgcaa aacaggcggc acagccctaa
     1261 aaggaggtaa cagcggaaat gttgtctata ccccgcccga aggagaagaa accatacgcg
     1321 gcaagctggc aaattgggag cggtttattc acgaaagcgg cgatttagac ccgcttgtca
     1381 tcatggcggc ggcacattac caatttgaag ccatccaccc gtttacggac ggcaacgggc
     1441 ggacgggacg catattgaac agcctgctat tgattgaaaa agggcttttg gatttgccta
     1501 ttttgtattt gagccgctac atcatcgaaa acagggcgga ctattaccgc ctgcttttag
     1561 gcgtaaccga acggcaggat tgggaaagct ggataatcta catcttagac ggcgtagccg
     1621 acaccgccga ttggacggta tcgaaaatag atgcgatacg ctgcctgttc gagcagacac
     1681 gtcaacacat acggacacac gcacaaggaa tctacacgca cgaactggta aatcttctgt
     1741 ttgagcagcc atatacacgc attgccaacc tagaagcggc agggatagcc aaacggcaga
     1801 cggcttctaa gtacctgaaa gagctttcag gcataggtgt gctgcaagaa atcgccatcg
     1861 gcagggacaa actattcatt catccgcgcc taatggaact attgcgggga gagggcaaca
     1921 gctttacttc attctaaccc cctcttcatc cctacatgac taacacgaaa cagggatttt
     1981 gacacccgaa ccggtacccc ttgtattttc cccgcgaaaa gccggcatcc gcccgcgcat
     2041 catgggagca acaaaacccc tgcctaaaat tttgacttgt gcaaattagg ggtatatttg
     2101 gggggtatat tgaaaaatgg ctaaaataaa atgtttaata aacaaaatgt tgaaacttaa
     2161 tttcgataga gcctctgcat atcgtattga ggcgttaatg gaatttgaga aagctatttt
     2221 taaataagaa aaggtaacta ttaaatagct acctttctaa aattaaatat caacacatcg
     2281 ttaaaacaca gccaaaaaaa accgaaaccg aacggtccgg attccctcct gcgcgggaat
     2341 gacggatttc aagattacgg tattgtcggg gatgatggga aacggtggga attgtgtaaa
     2401 aaatgccgtc tgaagccttt caggcggcat ttgcggcatt cggacattta aaaatcccgt
     2461 cattcccgcg caggagggaa tccgaataca tccgcacaga aacctgcatc ccgtcattcc
     2521 cgcgaaagcg ggaatctaga acgtaaaatc taaagaaacc gttttatccg acaagtttcc
     2581 gcaccgacag gtctagattc ccactttcgc gggaatgacg ggattttagg tctctgtaat
     2641 ctcgggcagc tttccgttat cgttttaacc caaagacacc atttcaatct gctccatcgt
     2701 cctgcccaga aaacgctcgc cgatggttct gaatttcaaa ggaatatcgc tgacgtaaaa
     2761 acggtagtcg ggattgttgt tgccggtatc gagcaatcct tcctgtgcaa ggacgcgtgc
     2821 ggtttcttcg gccgtcgtaa tcgcggaatc gaccagcgcg acattgtgcg cctccctgcc
     2881 gattaagggc ttgagcaagg gaaagtgcgt gcagcccaac accagcgtat cgatgccgtc
     2941 tgcaagcaat ggtttgaggt attcgcatac ggtcaggcgg gtaacttcgt gttccaacca
     3001 accttcctcc accaaaggga cgagcagcgg cgcggcctgc gtgcggacga gcgtgtcggg
     3061 gttgtccctg tggatggcgc gcgcataagc attgctgttg actgtcgtat tggtggcgat
     3121 gatgccgatt ttattgttgc gcgtcgttgc cagcgcggct ttggcgccgg cggaaatcac
     3181 gtccaaaacg ggcatattgc ccgtcttttg acggattttc cgccccgcaa ccgccgcaat
     3241 cgtgttgcac gcgataacca tcgccttgac atcgtgtccc aataaaaaat cgacaatctg
     3301 catcgagaaa ttttcgatgg tcgccttaga tttcgtcccg taaggcacgc gcgccgtgtc
     3361 gccgaaataa atgatgttct ccatcggcag ccgttccatc agcgcgcgca cattggtcaa
     3421 accgccgatt cccgagtcaa aaacgccgat gggccgctgc ctgccgatat tttccatcct
     3481 ttttccaatc cgtcctaaac atacaatccg catattgtac acgcgccgct ttttcttgac
     3541 agccatgccg tctgaaagca ggatgcgtat ttggctgtta taatgtacgg caatgccgcc
     3601 gccgggcgca cggtttgccg accgcctgcc cctccgatcc gccacataaa agttgtaaag
     3661 cccctatgtc tgatttttcc cccgtttccc gattccttgc cgacgaagcc gccacactcg
     3721 atttgggcgc ggcgtggtct tcccgtttaa acgcaccgct ggtcatttac ctcgaagggg
     3781 atttgggtgc gggcaaaacc acgctgacgc gcggcatcct gcgcggattg ggtcaccagg
     3841 gcgcagtcaa aagcccgacc tacgccatcg tcgaatctta tccgctggaa cgcttcgccc
     3901 tgcaccattt cgacctctac cgcttctcgt tccccgaaga atgggaagac gcggggcttg
     3961 acgagctgtt tgccgcaaac agcgtctgcc tgatcgaatg gccgcaacag ggaggcgaat
     4021 ttaccccgcc tgccgacatc accgcaacat tgacacacgg cggcggcggc agaaaatgcc
     4081 tgctgaccgc ccataccgaa cgaggacgcg aaagcctgcc gctatgacca aactgacacg
     4141 aagacaaatc atccgccgca ccgccggcac actgttcgcc ctaagcccca tcgcatccgc
     4201 cgttgccaaa acggtacgcg ccccgcagtt taccgccgca cggatatggc cgtcgcacac
     4261 ctacacccgc ctgacgctgg aaagcaccgc cgcgctcaaa taccagcact tcgcgctcga
     4321 caacccgggc aggttggtcg tcgatataca aaacgcaaac atcaataccg tattgcacgg
     4381 gctttcccaa aaagttatgg cggacgaccc ctttatccgc agcatacgcg cgggtcagaa
     4441 cacgccgacc accgtccgcc tcgtcatcga cctgaaacag cccacccacg cacaagtctt
     4501 cgcgcttccg cccgtcggcg gctttaagga ccgcctcgtc gtcgacctct atccgcacgg
     4561 gatggatgcc gacgatccga tgatggcgct gctcaacggc agcctgaaca aaaccctgcg
     4621 cggctctccg gaagccgacc ccgcccaaaa caccacgccc cgacccgggc ggggcaaaaa
     4681 cgggcgcaga cccgtcatca tgctcgatcc gggacacggc ggtgaagacc ccggcgccgt
     4741 cagcccgggc ggtttgcagg aaaaacacgt cgtcctctcc atcgcccgcg aaaccaaaaa
     4801 acaattggaa gccttgggtt acaacgtctt tatgacgcgc aacgaagacg tgttcatccc
     4861 attgggcgtg cgtgtcgcca aagggcgcgc acggcgggcg gacgtattcg tctccatcca
     4921 cgccgacgct ttcaccagcc cctccgcgcg cggaacgggg gtttatatgc tgaacaccaa
     4981 aggcgcgacc agctctgccg ccaaattctt ggaacaaacc caaaataatg ccgacgcggt
     5041 cggcggcgta ccgaccagcg gcaaccgcaa cgtcgatacc gccctgctcg acatgacgca
     5101 aaccgccaca ctgcgcgaca gccgcaaact cggcaaactg gtgcttgaag aattgggcag
     5161 gctcaaccat ctgcacaaag gcagggtgga cgaagccaat ttcgccgttt tgcgcgcacc
     5221 cgatatgccg tctatcctgg tcgaaaccgc cttcctgtcc aatcctgccg aagagaagct
     5281 gctgggcagc gaatccttcc gtcggcagtg cgcccaatcc attgcctcgg gtgtccaacg
     5341 ctacatcaat acatccgtat tgaagcgggg ttgattgaaa aggcggtgtt tcaaaatata
     5401 gttgtcggat gaaggcaggc ataaacctac ttccgcccca aaccgcgttt ttatgatgaa
     5461 aggtatggat aaattgagat accaacggga ttttttaaat atccgcccca tttttacagc
     5521 cggcgaacaa gaatacctga ccgaattgtc cgatcggctt cctttatctg ttttaacgga
     5581 ctccgtgcgt aatattgaag aaatcggtat cgattttgtg tacagtcctg ccaaattgga
     5641 aggaaacact tataatcaat acgatacgca ggcattgctt aaactcgggc aaaccgcagg
     5701 cgggaagtta tattcggatg ccgtaatgtt aatcaacctg agggaaagtt accggcacct
     5761 gctgtccggt ttggacagcc ccaagccgtt tgactggttg gatttcttaa aaaccaccca
     5821 cagcctgatt tcggaaaact tactggaaaa aggctccggc ggcgtagtcc gccgcgacag
     5881 cgttactata agcgggacag actacacccc tttatccaac ccgcaaagtt tggatacgga
     5941 attgaaatgg ttgcttcaag aagcccccaa aatagaaaat cctttcgacc gcgccgttta
     6001 tctccataat aatttggcgt atctccgata ttttaaggac tgcaataaac gtactgccag
     6061 aaactgtatg accttgtcgc tgatgcgctc cggttttttt ccttgcgtat tctcccccga
     6121 cagttatccc gcctatgccg aggctgtcgt agcctattac gaaaccggcg attacggttt
     6181 attcaagaaa tattttattt cggcatacga aaataccgta aataagtacg gtccccagcc
     6241 tgatgtggat attttccgaa atttctccat ttaaaaagcc gtctgaacat ttttcagacg
     6301 gcttttttgt acactttcct caacggttcg atccctgcac catatcgatc aggaacaggc
     6361 ggcagtcgag gttgccgtta taaagcggga ttttccgctt gggcagcagg cgcatgaatt
     6421 tgggcatttc cctgtcgccg gtaaacatgc cgacccgcca gccggcatag tgttttttca
     6481 accacgtccc caactgcgga taaagtgcct gcaaagcgcg gacttcctca aggcgcacgc
     6541 cgtagggcgg attggacacc atgatgccgt tttcgccgtt cggacggacg gactgcgcgc
     6601 cggcaacgct gaaggaaacg atgtcgtccg ctccggcatt gtgtgcgttg tccaatgccg
     6661 tctgaacgat gcggcggccg ttgtcgctgc ctgcaatcgg ggcgcggacg gggcgggttt
     6721 gcgcttcggc gcggcggcgc aaatccgccc acagcgtttt atcgaaattt tgcagttttt
     6781 caaaaccgaa gcggcgcatc atacccggcg cgcggccggc ggcaatccaa gcggcttcga
     6841 tagcaatcgt gccgctgccg caaaacgggt cttgaaacgg ctgcgtgccg tcgtagcctg
     6901 ccgagagcag cagcccggcg gcaaggtttt cgcgcagcgg ggcttcgccg gtatccaggc
     6961 ggtagccgcg tttgaacagg gcttcgcccg aagtgtcgat gaagatttcg acattgcgtt
     7021 cgtccaaaaa ggcgtggatg cggacatcgg gcgcggcttt gtccacgctc ggacgtgcgt
     7081 cgtaaatgtc gcggaaagcg tcgcagacgg catctttgac ggtcaggccg gcaaattgga
     7141 ggcttttgac gttggcgcgt tttgcctcga ttttgacttt gaacgtctgc tgcaaggtaa
     7201 accagttgaa ccagttgatg tttttggcaa gtttgtagat gtcgcgctcg gttcggtatg
     7261 tgcctttggt caggcgcagc aggatgcggc cggcggtacg cgagtgcagg ttggcggcgt
     7321 aggcctgttc caatccgccc cggcaggaaa cgccgccgtc aaacgcttgg acatcggtac
     7381 agccgaggct tccgagttct tgagataaaa cggtttccaa gccgcgcggg caggtggcga
     7441 aaagtgtata taaagtattc atagcgtgtc ctttccggac ggatgttccg gcaatggcga
     7501 attgaaaggt ttattcggcg gtaaatgccg tctgaaatgt ccggctgccg attgtaatgt
     7561 ccgccgtaaa atcgcgcctg ccttcgacac agacgggcag gcggatgcgt gctgcctgcc
     7621 aagtccccga cggttgccgc tcgaacatat agcggttgaa acccatatcc atatttttca
     7681 tactgaagct gatgctgacc tgttccgtgc cggcgggcgc gtgttcgata taaatatcaa
     7741 acggtttttt ggttgaaacg gcggcggcgc ggacacggct tccgtccggc agcgtgcaac
     7801 cctcggtcaa atcgcattgc gccgccacgg cttgcggctg ctgcgcctgc caccattgca
     7861 acaaaacgag cttgaaggcg gcaaaggcag tcagcagcag tgcggcaagc agtaatttgc
     7921 ggtttttatt catgattctt ccctattgtt tcatccctta ccaccgcgtg cgcccccaat
     7981 ccgagccata gttttccata acgtgcaacc gtctgtccgt ttgctgcaag tacgagcggc
     8041 acggatacgc cctgccgcag ggcttctgcc gcctcttgtg cctgaacatc atcgcacacg
     8101 cgccacacgg ccacatccgc gcccttttcc tgcgcctgcc gccattgttc gcgggtctgc
     8161 accatcatcc acacgctgcc ggcgtgttcg gacctgtcct gccattgtgc cgcctccatc
     8221 aaaacgttcg caccgcttcc ccctgccgaa cccaaaggca ggacgcggta cgcgcccata
     8281 ctgttttcgc cgtacaaacc tgttttcagg ctgccgtaca aacggcgcgg gacggacagc
     8341 gaacgcaaaa gcgcaccgtt ggcgggcagc atgggggcaa cggtaaaatc acccgccttc
     8401 tgccaagacc attcctgccc ttcgcgcgat tgcggtccgc cctcccattg atcggggttt
     8461 acccataaga atttcaggca gacgcgggcg tgttcgtagg aatggatttt ggtcagccaa
     8521 ggcgtggcgg cgaggatgcg gatgccgagt tcttctccaa actcgcgttg cagggcttgg
     8581 aagtcggttt cgcccgcctc gaccttgccg ccggcaaatt cccaatatcc ggcatagggc
     8641 ttgccttcgg ggcgcgagcc gagcaggtaa ttgccgtctg aatcgagcag gatgccggca
     8701 acgacgcgga taagggggcg ggtatcttgg atcatggcgg gtatgcggtt ggtcggtcgg
     8761 aatgcaaatg agggaagggc aatgccgtca acgggtttgc cgaatttcag acggcattat
     8821 ttttcggcaa cggcaaacgc caacacggta tcgccttcgt ccgccatact caggctgacg
     8881 cggctgatgc cttgttcttc cagccattcg gacagggcgg ggccgtagaa aaattcgggc
     8941 ttgcccaatg cgtcgtgccc gatgccgatg ttgcggaagg aaaccgcgcc tcgtatgccc
     9001 gtgccgacgg ctttggcgaa ggcttctttg gcggcaaagc gtttggcaag gtagttgacg
     9061 ggtttgcccg cctgcggaaa ttcaagcagc tcttcgggag tgagtatgcg ccctgcaaac
     9121 gcctgtccga attttttgtt taagcggatg atgcgcttga gggaaacgat gtctgtaccg
     9181 atgccgtaaa tcatatttgc gctccttttt cgtgaaatgc cgtctgaaac ggtttgcctg
     9241 cccttacggc agcagccttg ccctgaacat cgcctccttc atctggcgca cggcctcggg
     9301 caatccgagg aaaagtgcct gcgaaatcag cgaatgcccg atgttcagct cgcggatggc
     9361 gaggatttgg gcgacggggg taacgttgtg tatggtcagt ccgtgtccgg cgttgacgac
     9421 caagcccaaa tcgccggcga aatgcgcgcc gttttgcaga cgctcgaact gcctgatttg
     9481 ttcggcgtgg ctgtgtgcgt cggcatatgc gccggtgtgc agctcgacaa cgggcgcgcc
     9541 gacatcacgg gctgcttgga tttgctcgtt gtcggcatcg atgaagagcg agactcggat
     9601 gcctgcgtcg gtcaggattt tggtgaaccc ggcaattttt tcttgttgcg ccaatacatc
     9661 caagccgcct tcggtcgtga tttcctgccg tttttcaggc acgatgcaca cgtcttccgg
     9721 catcacttta agcgcgtttt cgagcatttc ttccgtcaac gccatttcaa ggttcaggcg
     9781 cgtgcggatg gcgtttttga cggcaaatac atccgcgtct ttgatgtggc ggcggtcttc
     9841 gcgcaggtgc atggtaatca ggtctgcacc gtgcgtttcg gcaaccagtg ccgcctccac
     9901 ggggctggga taagtcgtgc cgcgcgcgtt gcggacggtg gcgatgtggt cgatgttgac
     9961 acccagaagc ataatgtttc cctttctctt cgttttcctg tttgacggca tggtgcgggc
    10021 gcagctacgc ggtattgcgg tcgaactgcc ttatctgttc caacacctgc cgcgatttca
    10081 agccttcggg aagcagcgtg ccgattagca gccgtgtgat tttcaatgcc tgttgcaggc
    10141 tttcggcggt gcggaaactg ccttcgcgca aatcgatcag gctctgccct tcgacaattg
    10201 cgccggcttc gtgcgaaagc gcgcctgtgt cccggcatac gggcatgacc gcctcttcgg
    10261 gcatcagccg gtaggttttg tccgccaaaa tatcgccgcc cgtgccgtcg gcgtgcaaat
    10321 cgggggcaac gcccaaggca ttgagcagct tccattcgaa gcggcgcaaa tcggcaatat
    10381 ggttggcttc acggcacact gcctccatca cttttgccaa cgcgtcgtac agttcggaca
    10441 tcgggtcttc gcgcgcggtc agtttcaaca ccagctcgtt cacatacaga ccgctgaaca
    10501 acgccctgcc ctgcggctgc cgccatccgc ccatccattc ggcgcggtgc agggttttga
    10561 gttcctgact gccgtaccac gacacgctgg cagggacgaa cggaaccaat acgccgcgca
    10621 gctcgctctg ccgtttgcgc gcgctgcgcg ccagcaaagc cacacgcccg taacggcggc
    10681 tgaatgcttc aacccgcagg ctgctttcgc gccaaggcga agatgccagc ataaaaacgg
    10741 gttcgtggtt gactcggtat tcggacataa tcgttgtgga aatgccgtct gaacgctgcc
    10801 gcgactacaa aacggcagtc ggatacgaac cgatggcttt gacaaacgaa gcgcgttcgc
    10861 ccaagcgttc caatgccgtc tgaatctgcg cgtcccggcg gtgtccttcg atgtcgatga
    10921 agaacaggta ttcccacaaa acggatttgc tcggacggct ctcaaacttg gtcatggaaa
    10981 tacccgattc ggtcagcggt tgcagcagcg aggcaaccgc gccggcgcgg ttgggcgcgg
    11041 aaacggccag cgaggttttg tcgctgccgc ttgcaccggt ttcgtgatgt cccatgacca
    11101 gaaagcgcgt ggtgttgttc ggttcgtctt cgatgcactc ggcaaccata tcgagtccgt
    11161 aaatttccgc cgccgtgcgt ccggcgatgg cggcaaccgt accgtcgtcc gattcggcaa
    11221 ccagccttgc ggcttcggca ttgctggaca cggcaatccg ttcggcgttg ggcagacgcc
    11281 tgcccaacca gtcgttgcac tgcgccaacg cctgcgcgtg cgaaaatact ttggcgatac
    11341 cctcggtgct gccgttgttt ttgcgcaaca ggttgtggtg gatgcgcaaa acgacttcgc
    11401 cgcacgcctg caacgcggta acggcaagca ggtccaacgt gcggccgacc gagccttcgg
    11461 tcgagttttc cacaggggcg accaaataat ccgcctgacg ggtttcaacc tgtttgaagc
    11521 agtcgtctat ggtcggacac gccattgtat gcgcggcgtg tccgaaatgc ttgattgccg
    11581 cctgttgggt aaacgtgccc tgcggcccca gataggcgat ggtcagcgga cgttcgacgg
    11641 cgaggcactc gctcatcact tcccgaaaca ggcgtgctac cgattcgtcg ggcagcgggc
    11701 ctttgttcaa atcctgaatg cggcgcaaca cggcaacctc gcgttcgggg cggtacaccg
    11761 cgcccgtgcc tttcagctcg ccgatggcgt gggcatgttg cgcgcgttcg ttgagcaggc
    11821 gcagaatttc ggcatcgatg gtgtcgatgg cgttgcggtg cgggatgagg agttcgtcga
    11881 tagtttggga cataatttcg cctgaacggt aatttgccgt gcattctaac atcaaatgcc
    11941 gtctgaagcg gtttcagacg gcattttccg ttaaaggtat tcgctttgcc cgttttcttt
    12001 ccacgcacga tgcgagcaga gccacaaaag cgcaatgccg caagaggtag ggccgccata
    12061 gttgccgcca tcacagttgc cgaaccgtcg tgcaggacgg ttgccgccat gcccacgccc
    12121 gcgccgatta aggaccggaa tacacccgac acggcgttcg cgctgccgcc ctcttctttg
    12181 aaataagaca taaagcatgc ctgcgtgtcc gcaccgacca ggccctgcgt accgacggaa
    12241 aacatcacgc acgcaaccgg cagccaaaac gggggcaacc cgaaaaacag cacggcggcg
    12301 agttgggacg ggttggcggc aaattggacg acaatccccc gcagcaggat gctttgcgga
    12361 tgcgcgccgg ttttaagccg ccacgcggta acgcggctga aaaacatcat cgtgatgatg
    12421 ttgagtgcaa acacccatgc gtaccggtgc ggcgtaacgt ggtagagctg ccggtacacg
    12481 aaggaagatt cggtcagaaa ggcgaacatc gaaccgaagc tgaatgcctg aaaaaacaga
    12541 taacccatcg cggcacgggt tttcaatacg cgcttgaacc gccccgccac cagcccgaac
    12601 acatccctgc cgattttgcc gccgacggcg ggattgggca ggaaatactg taccaaaccg
    12661 ggcagcaccg gcgaatacgc cgccaagaaa acgaaaatcg cccgccatcc gcccaatccc
    12721 tgcaacaatg cgccgaccat gggtccggcc agcggcacaa ccatcagaat gatgccgata
    12781 agggcaaaca tctgcgcggc tttgcgtccg gaataataat cgcgcaccat cgcaccgacg
    12841 atgactacag ccatgcctgc gccgaacgcc tgtaccgcac gcaggttaag gagctgttcg
    12901 gtactcgaag caaatacgat ggcggcaacg gcaaggcaat atacaatcaa accggtcagg
    12961 gcgacgggtt tgcgcccttt gatgtcggac accgcgccgc cggccacttg cccgaacgcc
    13021 gtgccgaaca taaacaaact cagactctat tcgatacggt ggatatccgc gttcagcggc
    13081 tgcgccattt cgggaatcgc gggcaggtag gcatcggtgg aaaacggcat cagcgcgacc
    13141 agtaccgcca tcaaaaccgc catcagtttt ccgctcatcg gaggaagggt cggagacatg
    13201 gtttttccgt tcaaatcaaa taaagaaacc gtcgcaaacg acggtcggta cgggcgcaag
    13261 tttaacatat cggacggcgg ggcaaaaccg aaggatatgc cgcccgaaac cgcttcagac
    13321 ggcattgccg ttttgcgcgt ccctgcgcga cagccggccg gcggcgaaag tggcggcgat
    13381 gacgacaaac acgcccaagg cggacacggg cgataaatgt tcgcccaaaa tcaaaaccgc
    13441 caacagcacg ccgacgacgg gttcgagcga aatcaacagt cccgacgcgt tggcaggaac
    13501 acggctcatc cccttgttcc acagccaata ggcgtaccag ccgcacccca aacccaaata
    13561 caacagcgac aataccatcc cgacgctcca gtccacggta taactttgcg ccaaagcaag
    13621 cgaaaacggc aggcacatca acgatgcggc ggcaatggaa acagatgtga atgccggtgc
    13681 gccgatgcgg gcaatcagcc tttgcgtcgg acgcatagcg gcacaaaagc ccgcgcccgc
    13741 caacaacacc agcaggcagc cgaaccagcc gacttcgccg ccctcttccg caccgcccgc
    13801 catcagcagc gcgacaccgg caaatgccgc cgcgccgcat atccagtggt aggcacgcgc
    13861 tttgtcgttg aagaaaaagt gtccgacaaa caccatcagc agcggctcga gtccgacaat
    13921 gaccgatgcg ctggcggcgg aagtgtattt caacccgaca aactgaagca gcagggtcag
    13981 cacatagttg acgaacgaca caatcagcaa cggcttccat tcctcacgcg gaatcttgcc
    14041 gacatgacgg cggcaggcgg gcagtgcagg cagcgcggca atcagcaggc gcacgccgac
    14101 catcaatgcg ggatcgatgc cgccatagac atatttggcg gcaataaacg agctgcccca
    14161 gataatcagg gcaaggattt ggtaaaacat agtgagtggt aaagtcagat ggtcatacaa
    14221 aagtatagcg gattaacaaa aatcaggaca agaaggaatg cccgaaccgt cattcccgcc
    14281 acttttcgtc attcccgcga aagcgggaat ctggaatctc ggactttcag ataatctttg
    14341 aatattgccg ttgttccaag gtccggattc ccgcccgcgc gcaagtttcc gaagccatcc
    14401 ttttggccga aggtcaaaaa tcagccgtca ccgagtatta cctgaatcac ggcatatggc
    14461 cggaa
//
LOCUS       DAXDXW010000065        14271 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXDXW010000065 DAXDXW010000000
VERSION     DAXDXW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14271
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.066"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(57..1157)
                     /locus_tag="ACFB64_001636"
     CDS             complement(57..1157)
                     /locus_tag="ACFB64_001636"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC1209964.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRKLLDLQDTGTDELHRSRMLSQELSRLYAQTAAELLCSQNLAPCDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRE
                     TRVVLNIGGIANISVLPPGAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLGRLLAHPYFSQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            1482..3056
                     /locus_tag="ACFB64_001637"
     CDS             1482..3056
                     /locus_tag="ACFB64_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC1209965.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTD
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASRKGLPVSTTHAIIGGIVGSA
                     LCMAFTHNADGVALIRWGKLGEIGMSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGTMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQSAVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSMASYLILQAVF"
     gene            3070..3258
                     /locus_tag="ACFB64_001638"
     CDS             3070..3258
                     /locus_tag="ACFB64_001638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1209966.1"
                     /translation="MPSEPVFRRHFVDGMCGLVPYAQSVLSGYAVWYLGFGTVGHKGY
                     DMPPPVLENTYPRQQNAV"
     gene            complement(3296..4717)
                     /gene="hemN"
                     /locus_tag="ACFB64_001639"
     CDS             complement(3296..4717)
                     /gene="hemN"
                     /locus_tag="ACFB64_001639"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC1209967.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKVLHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADTYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPSGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSVGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            4852..5586
                     /gene="fnr"
                     /locus_tag="ACFB64_001640"
     CDS             4852..5586
                     /gene="fnr"
                     /locus_tag="ACFB64_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC1209968.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNEFSQLDAVIRQSR
                     RLKKGEYLFRAGGAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSYV
                     HSCDAVALENSEVCELPFTHIEELGQNIPSLRTHFFRMISREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            complement(5699..6649)
                     /locus_tag="ACFB64_001641"
     CDS             complement(5699..6649)
                     /locus_tag="ACFB64_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC1209969.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGR
                     LSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFAKARLE
                     RPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            7013..7723
                     /gene="rmpM"
                     /locus_tag="ACFB64_001642"
     CDS             7013..7723
                     /gene="rmpM"
                     /locus_tag="ACFB64_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC1209970.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAVPEPEPAPVAVVEQAPQYVDETISLSAKTLFGFD
                     KDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSEKYNQALSERRAYVVANNLV
                     SNGVPASRISAVGLGESQAQMTQVCQAEVAKLGAKASKAKKREALIACIEPDRRVDVK
                     IRSIVTRQVVPARNHHQH"
     gene            8498..9454
                     /gene="thiL"
                     /locus_tag="ACFB64_001643"
     CDS             8498..9454
                     /gene="thiL"
                     /locus_tag="ACFB64_001643"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC1209971.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEGLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTCIGKINGGCRLKVLDAGGRELELHSLGFDHFG"
     gene            9447..9932
                     /locus_tag="ACFB64_001644"
     CDS             9447..9932
                     /locus_tag="ACFB64_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC1209972.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGAFGTLAALPLA
                     FVLILLGIDGLLLAFVCIVLFMWGIRICACAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPPPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(9992..11668)
                     /gene="ettA"
                     /locus_tag="ACFB64_001645"
     CDS             complement(9992..11668)
                     /gene="ettA"
                     /locus_tag="ACFB64_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC1209973.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGT
                     GKSTVLRIMAGVDKEFEGEAVPMGDIKIGYLPQEPELDPEKTVREEVESGFGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPDWD
                     AKIGNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKPKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIARQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
     gene            11904..13258
                     /locus_tag="ACFB64_001646"
                     /pseudo
     CDS             11904..13258
                     /locus_tag="ACFB64_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
     gene            13262..14223
                     /locus_tag="ACFB64_001647"
                     /pseudo
     CDS             13262..14223
                     /locus_tag="ACFB64_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
BASE COUNT         2987 a         3595 c         4051 g         3638 t
ORIGIN      
        1 aaaggggtgg aaatgccgtc tgaacggcgg aaatggcttt cagacggtat ttgcgctcaa
       61 taataatatc ccgcgcccag aatacacggt ttggatgcgc cggtcgcttt gtgcggacta
      121 ccgggaatgc ggttaatcca acacgccgcc aaccacgcaa atgcggccgc ctccacccat
      181 tgaggatcga ggttcagttc ggcggtgctg tgcagggaaa cgcgtgtgcc gaaacattct
      241 gccaaatccg ccattaaaac aggattgcgg atgccgccgc cgcaaatgta catttgacgg
      301 gcatctgccg ctgcgtgtga gacggcgtcg caaacggttt gcgcggtgaa tcgggaaagc
      361 gtccgcaata cgtcgtatcg gttttcgccg ccgtcaaggt aggtttcgag ccaatttagg
      421 gcaaacagtt cgcgccccgt gctttttggg tggggttgtg agaaatacgg gtgggcgagc
      481 agcctgccga gcagttgcgg caatatgttg ccttgtgccg cctttgcacc gtttttgtcg
      541 taaggcagct gccagtgtgc ctgcgtccac gcgtccatca gcatattgcc cggccctgtg
      601 tcgaagccga aggcgggtgc gccggggggg agtacgctga tgttggcaat cccgccgatg
      661 ttcagtacca cgcgtgtttc cctgtcatcg cggaacaggg cttcgtgaaa ggcggggacg
      721 agcggcgcac cttgtccgcc ggcagcaagg tcgcggctgc ggaagtcgcc gacggtaaaa
      781 atccgcgtca gttccgccag cagcggcaaa tcggcaagct gtatgctgta accgtgttcc
      841 ggcgcgtgtc ggacggtttg cccgtggcag ccgagggcgg taatgtcgca cggagcgagg
      901 ttttgactgc acagcagttc ggcggcggtt tgcgcgtaca ggcggctgag ttcttgcgac
      961 aacatcctgc tgcggtgcag ttcgtctgtg cctgtgtcct gcaaatccag caatttgcgg
     1021 cgcaaccggt cagggtaggg ggtaaaggcg tgcccttccg cgcccagcca tttgccgccg
     1081 tccatccgta ccagcacggc atccgccccg tccatactgg ttcccgacat aatgccgatg
     1141 taaagctgtg tttccatcat cactcccaaa ctggtgcaaa acgccatttt aacgtgtatt
     1201 gacgctcgta taccgatttg ccgccgcagt gtaaataaag tgtaaataaa tgttttaaga
     1261 cgggtggaaa aatattataa tgcgcccgca acatccagta gtagaagtgt catacaaacc
     1321 gtttccggca gcagttttgc attcggtcag gtttgggggt attcggatgc ggttcagaag
     1381 gaaggatgcg tctgccatat cccgaaacgg cagttcgacc ggaggcagca atacagtgtc
     1441 ggcaacactc atgatttcca ccacattaaa ggaagattgc catggctcaa atccaaatga
     1501 gcgcaaatgt taaaaccatc aacgccgtct ttgccgccat gctggtaggt acagtcggct
     1561 attttattta ttggggcttg ggttataccc attacaatta cgccgcctta ttcattattg
     1621 ccacgatgtt cggtgtgttt atggcgttca acatcggcgg caacgatgtt gccaattctt
     1681 tcggcaccag cgtcggtgca ggcacgctga ccatcccgca ggctttgctg attgcggcgg
     1741 tatttgaggt cagcggcgcg gtcatcgcgg gcggcgaggt aaccgatacc atacgcaaag
     1801 gcatcgtcga tttgaagggt gttgatttcg aacccataca gtttgtgttt attatgatgt
     1861 ccgcgctttt ggcggcggcg ttgtggctgc tgttcgcgtc ccgcaaaggc ctgccggttt
     1921 cgacgacgca cgcgattatc ggcggcatcg tcggcagtgc gttgtgtatg gcgtttacgc
     1981 ataatgcgga cggggtggcg ttgatacggt ggggcaagct gggcgaaatc gggatgtctt
     2041 gggtattgtc gcccgtgttg ggcggcgcgg tgtcctattt cctgttttcg cgcgtcaaga
     2101 aaaacgtctt agattacaac gcttgggcgg aaggaacgct caagggcatc aagcaggaaa
     2161 aaaaggccta taaagaacgg caccgcctgt ttttcgaggg tttgtccgaa gccgaaaaag
     2221 tcgagtacgc caccaaaatg gcgcacgacg cgcaaattta cgacgaaccc gaattcgatc
     2281 ctcaagagct gcaatcggag tattaccgcg gtctttatgc gttcgacaac cgtaaaaaca
     2341 atgtcgattc ctacaaggcg ctgcattctt ggattccctt tatcgcttcg ttcggcacga
     2401 tgatgatttc cgctatgctg attttcaagg gcttgaaaaa cctgcatttg gggatgagca
     2461 acgtcaacag cttcctgacc atctttatga taggcgcggc ggtgtggatg gggacgtttg
     2521 tgtttgccaa aagcctcaag cgtaaagact tgggcaaatc gacctttcag atgttttcat
     2581 ggatgcaggt ctttaccgcc tgcggcttcg cattcagcca cggtgcgaac gatatcgcca
     2641 acgccatcgg cccgtttgcc gcgattatgg atgttttgcg gacgaacagc gttgccgcgc
     2701 aaagcgccgt ccctccgatt gcgatgctga ctttcggcat cgcgctgatt gtcggtttgt
     2761 ggtttgtcgg taaagaggtg attaaaaccg tcggtacgag tttggcggaa atgcatcctg
     2821 cttcgggttt taccgccgaa ctgtccgccg catccgtcgt gatgggcgcg tcgctgatgg
     2881 ggctgcccgt gtccagtacg catatcttgg tcggcgcggt actcggtatc ggtctggtca
     2941 accgcaatgc caactggaaa ctgatgaaac ccatcggttt ggcgtgggtc attaccctgc
     3001 ctgccgccgc tgtattgtcg atggccagct atttgatttt gcaggcagtg ttctgattgt
     3061 aaaatactga tgccgtctga acccgtgttc agacggcatt ttgttgatgg aatgtgcggg
     3121 cttgtgcctt atgcacaatc tgttctgtcg ggatatgctg tttggtatct tggtttcgga
     3181 acggtcggac acaaaggtta tgatatgccg ccgcctgttc ttgaaaacac ttatcctcgg
     3241 cagcaaaatg ccgtctgaaa aagcctttca gacggctttt gtacgttagc cgcaatcaca
     3301 ccgtttgcga atatttcgct ttggtttctt tgtggcgcag gtggtagtcg aagaccatgg
     3361 cgatgttgcg gatgaggaag cgtcctttcg gtgtaacggt cagcccgtgg ctgttcaggc
     3421 gcaccaatcc caaaccggcg agtttttcca aatccgccag ttcgtctttg aagtagcggt
     3481 cgaacgggat gccgaacata ctttcgtaaa tcctgtagtc gagcgcgaag cggcacatta
     3541 aatcctgaat gatgttgcgg cgcaggatgt cgtcctgatt gagctggtag ccgcgcatga
     3601 tgggcagtct gccttcgtcg atggcggcat agtaggcatc gatgtcgcgt tcgttttggg
     3661 aataggtgct gccgattttg ccgatggacg acacgccgat ggcgaccaaa tcgcaatccg
     3721 cgtaggtcga atagccttgg aagttgcgtt ggaggaagcc ttctttgagg gcgatggaga
     3781 gttcgtcgtc aggtttggcg aaatggtcca tgccgatgaa gacgtagccg cgctctgtca
     3841 gggtttggac gcagtattgc agcatatcga gcttctcttc gctgtcgggg acggcggcgg
     3901 tatcgatgcg gcgttgcggt ttgaacacgt gcggcaggtg ggcgtagtgg taaagggcga
     3961 ggcggtcggg atcgagcgac aaaacggtat ctatggtggt tttgatgctt tcggaagtct
     4021 gatgcggcaa gccgtaaatc aaatcgacgc tgacggattt gaaccctgcc tcacgggcgg
     4081 catcgatgac ttctttggtt tcttcgtagc tttggatgcg gttgactgcc gcctgcactt
     4141 tggggtcgaa atcctgaatg ccgacgctca tgcggttgaa gccgagtctg ccgagcatga
     4201 ggacggtgtc gcggctgact ttgcgcgggt cgatttcgat ggaatattcg ccggacggta
     4261 tcagttcgaa atgtttgcgg atcatgcgga agacacgttc gatctgttcg tcgctcaaaa
     4321 aggtcggtgt gccgccgccg aagtgcagtt gggcaagctg gtgccgtccg ttcagatgtg
     4381 gagcgagcag ttccatttct ttttcaagat attcgatgta ggtatcggcg cggcttttgt
     4441 ctttggtgat gattttgttg cagccgcagt agtagcagat ggtgttgcaa aacggaatgt
     4501 gaatgtaaag ggaaagcggt ttgtttaacg cgcccatacc gcgcaaatgt aaaactttga
     4561 tatattcgcc ttcgcggaaa ccgtcatgga aacggtcggc ggtagggtag gaagtatagc
     4621 gcgggccgct ggcgggcagg ctggcaatca gcgcgcggtc aaactcgggg cggtcatcgt
     4681 ttacattgtg attgttctgt atctgaatga ttttcatggt gtgtgtgcgg ttttatgatg
     4741 ttagtcaaat tttggatagt ttggtagaat gccacagtat gataaacctg tcttgatatg
     4801 tgtcaacaag cacataatcg ggatggggca aaacacacga aaaggtacgg tatggcttcg
     4861 cataatacta cacatcagat gaaaacgctg tgttcttcct gttctttgcg ggaactctgc
     4921 ctgcctgtcg ggctgctgcc caacgagttc agccaactcg atgccgtcat ccgccaaagc
     4981 cgccgcctga aaaaaggcga atacctgttc cgtgccggcg gagcctttac ctcgctcttt
     5041 gccatccgtt cgggcttctt caaaacaacc gtcgccagtc aggacggccg cgatcaggta
     5101 acgggtttct ttatgtcggg cgaactcatc ggcatggacg gcatctgttc ctatgtgcac
     5161 agttgcgacg cggtcgcctt ggaaaacagc gaggtatgcg agttgccgtt tacccatatc
     5221 gaagagctcg gtcagaatat ccccagcctg cgtacacact ttttccgcat gataagccgc
     5281 gaaattgtgc gcgatcaagg tgttatgctg ttgttgggca atatgcgcgc cgaagagcgg
     5341 attgccgcct tcctgttgaa cctttcccaa cgcctttatt cccgaggttt cgccgccaac
     5401 gacttcatct taagaatgtc ccgcgaagaa atcggcagtt atctcgggct gaaacttgaa
     5461 accgtcagcc gcacattatc caagttccat caggaaggat tgatttccgt cgaacacaaa
     5521 cacatcaaga ttctcaatct gcaggtgctg aaaaaaatgg tatccggttg ttcgcacgcc
     5581 atttgattaa cctgtacgaa catttcagac ggcattctca ataaacacag ggcagacgaa
     5641 aacatctgcc ctgtttgttg tatctgccgc aaagtgccgc ctgaaaaccg gcagccgcct
     5701 aaatcgaaaa atcctcgctg atgggcgtgt acagaatcct atccaccttc tcgcgtgtca
     5761 ggtgcggcgc aaacgcttgg ataaagtcgt aggcatatcc gcgcaaataa gtatcgctgc
     5821 gcaaagctat ccacgtcggc gacggctcga acaggtgtgc cgcatccaca agctgcaaat
     5881 cgccgtccgt atccgggttg tatgccattt tcgccatcag tcccacgccc aaacccaagc
     5941 gcacataagt cttcaataca tccgtatccg ccgccgccaa tgcgacatcc ggccgttcca
     6001 aacgggcttt ggcgaatgcc cgcgcgatgc tgctgcccgc attgaatgca aattcataag
     6061 taatcagcgg aaacctcgcc aaatcttcaa tacggagggg attcctgcat tcgagcagcg
     6121 gatggtcgtt cggcacaatc accgcgtgtg tccagtcgta gcaggaaagc cttcccaact
     6181 cggggtggtc gtctatccgt tccgtaacaa tcgccaaatc agattctccc gaagtaacca
     6241 tttgggcgat ggcggcaggg cttccctgtt tgatggtcag attgactttc ggatagcgtt
     6301 tcacaaaatc ggcaacaatc aacggtaggg catagcgcgc ctgcgtatgc gtcgtggcaa
     6361 ccgtcagcga accgctgtcc tgtccggtaa actcgctgcc gatattttta atgttctgta
     6421 catcgcgcaa aatacgttcc gcaatatcca aaaccacctt gcccggctgc gagaccgaaa
     6481 ccacgcgctt gccgctgcgg ataaaaatct gaatgccgat ttcttcttcc agcaatttga
     6541 tttgtttgga gatgccgggt tgtgatgtga ataaggcttc ggccgcttcg gaaacgttca
     6601 ggttgtgccg gtaaacttct aaggcgtatt tcaattgttg taatttcatg gcgggtcggt
     6661 gtgggtctgt gtcgggtggc tgaacattgt ttataattta tcatattttc ttgccggtac
     6721 ggtatggggc tttgccgttg tgtttgttgt ttttgtgcaa cggcaatcgt gcgatatgga
     6781 aaaaatcccc ctaaagtaat gacacggaat tgatttttcg gcatgataga ctatcaggaa
     6841 acaggctgtt ttacggttgt tttcaggcgt tgagtattga cagtccgccc cctgtttctt
     6901 tatagtggag actgaaatat ccgatttgcc gccatgtttc tacagcggcc tgtatgttgg
     6961 caattcagca gttgcttctg tatctgctgt acaaatctaa tgagggaata aaatgaccaa
     7021 acagctgaaa ttaagcgcat tattcgttgc attgctcgct tccggcactg ctgttgcggg
     7081 cgaggcgtcc gttcagggtt acaccgtaag cggccagtcg aacgaaatcg tacgcaacaa
     7141 ctatggagaa tgctggaaaa acgcctactt tgataaagca agccaaggtc gcgtagaatg
     7201 cggcgatgcg gttgccgtcc ccgagcccga acccgcgcct gtcgccgttg tggagcaggc
     7261 tcctcaatat gttgatgaaa ccatttccct gtctgccaaa accctgttcg gtttcgataa
     7321 ggattcattg cgcgccgaag ctcaagacaa cctgaaagta ttggcgcaac gcctgagtcg
     7381 aaccaatgtc caatctgtcc gcgtcgaagg ccataccgac tttatgggtt ctgaaaaata
     7441 caatcaggct ctgtccgaac gccgcgcata cgtagtggca aacaacctgg tcagcaacgg
     7501 cgtacctgct tctagaattt ctgctgtcgg cttgggcgaa tctcaagcgc aaatgactca
     7561 agtttgtcaa gccgaagttg ccaaactggg tgcgaaagcc tctaaagcca aaaaacgtga
     7621 ggctctgatt gcatgtatcg aacctgaccg ccgcgtagat gtgaaaatcc gcagcatcgt
     7681 aacccgtcag gttgtgccgg cacgcaatca tcaccaacac taaggctagg taatatcttg
     7741 ccgatgcatg aggttagcgg attttgtacc gggtactgtt gcaatattcg tgaaacgtcg
     7801 gccggtatcg atgatgtgaa acaaaccccg cttttgcggg gtttgttttt ttgggtggtt
     7861 ttctgaaacg gctatcgtca gaatcggggt gcaggttcgg attcggattc agattcatgt
     7921 ttgtgtccca ttgccgcgct ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
     7981 cgcagacagt acaatagtac ggcaaggcga ggcaacgccg taccggttta aatttaatcc
     8041 actatatcgg ttgaaactct gattttaagg cggtaggatg tgggtttgcc catagcaagg
     8101 gaatcctttc tgtatcaagc cccgaaaggg ataattcata caaattcacg cctttccccc
     8161 tcattgggaa atggatggaa tcgtgcccga tgtgtgcggc actgtatgcc ggatatggtt
     8221 ttatcatcat cccttttcgg ttgaaacccc gccagttgca gcgattgagc ctaatcggtg
     8281 gcggaagttg ccgctttgca ttcggggcgg cgtgcagtgc ggtgctttga tatgccgttt
     8341 gtgtgttgaa acagggtggt cggtgcatac gggtacggta tggctaaagc taaaagtgaa
     8401 atacgctgaa acactgaatg agccgcttta ttgtttgtac gacctttgct gctttgctat
     8461 gatttaaatt ggattcgcac gccggatatt ttgggatatg aaagaattcg acttcatcaa
     8521 acggtatttg caaacaggta cggataagga tgtcgtattg ggcataggcg acgatgcggc
     8581 gattgtccgc ccgcgcgaag gcttcgattt gtgtttcagt gcggatatgc ttttgaagga
     8641 caggcatttt tttgcagatg tcaaacctga aggcttggct tggaaggttt tggccgtcaa
     8701 tatttcagat atggcggcga tgggtgcgat accgcgttgg gtgctgctga gcgcggcgtt
     8761 gcccgaattg gatgaagtat ggctggaacg gttttgcggc agctttttcg gtttggcaaa
     8821 aaagtttggc gtaacgttaa tcggcggcga tacgaccaag ggcgatatgg cgttcaatgt
     8881 aaccattatc ggcgaattgc cgaagggtag ggcgttgcgg cgtgatgcgg cggttgcggg
     8941 cgacgatatt tgggtgtcgg ggcgtgtcgg tatggcggcg gcggctttga actgccgtct
     9001 gaaacggtgt gtgttgccgg atgatgtgtt cgccgaatgc gaacaaaagc tgctccgtcc
     9061 cgagccaagg gttgggctgg ggcttgcgct gttgccgttt gcccgggcgg cacaggatgt
     9121 ttcagacggc cttgcgcaag atttggggca tatcctgacc gcttccggcg tgggggcgga
     9181 aatttgggcc gattcgctgc catctttatc tgtattgaaa gatattttgc cccgagcgca
     9241 atggctgtct tatactttgg cgggcggcga cgattacgag ctggttttta ccgctccgga
     9301 aagttgccgc agccgcgtgc ttgatgcggc ggaacggtgc ggcgtgccgg taacgtgcat
     9361 cggcaaaatc aacggaggat gccgtctgaa ggttttagat gccggcggca gggaattgga
     9421 actacattct ttaggattcg atcattttgg ctgattttaa acctgacttt gcgtggctgt
     9481 tgaaacggcc gttgtgtttt ttggctttcg gtttcggcag cgggctggct ccgtttgcgc
     9541 cgggcgcatt cggcactttg gcggcactgc ctttggcgtt tgtgctgatt ttgctcggca
     9601 tagacgggct gctgctggct tttgtgtgta tcgtgctgtt tatgtggggc atacgtattt
     9661 gtgcgtgtgc ggaacgtgaa acgggtgtca gtgaccacgg tgggattgtt tgggacgaga
     9721 ttgtcgccat gctgtttgtg ctggcgtttg tgccgttcag gtggacgtgg tggctggcgg
     9781 catttgttct gttccgtctg tttgatgcgc tcaaaccgcc ccccgtcggt tggtttgaca
     9841 agaatctgca cggcggtttg ggcattatgg cggacgatat ggcggctgcg gtgatgacct
     9901 tgattgtctt gaggattgca atgctgtttt aaacggggct gccttgtaaa aatgccgtct
     9961 gaaagccttt cagacggcat tgtttcggag gttaacgcgt taccggtttg tatttgatgc
    10021 gtttcggttt cgcgccttct tcgccgagtc ggcgtttctt gtcggcttcg tattcttgat
    10081 agttgccgtc gaagaacacc catttggagt cgccttcaca cgccaagata tgcgtggcta
    10141 tgcggtcgag aaaccagcgg tcgtgcgaaa tcaccatcac gctgccggca aattccaaca
    10201 atgcgtcttc caacgcgcgc agggtttcca cgtcgagatc gttggacggt tcgtccagca
    10261 gcaacacatt gccgccgccc aacaaggttt ttgccaagtg cagacggccg cgttcgccgc
    10321 cggaaagctg ccttgcgatt ttgctttggt cgctgccttt aaagttgaag cgtcccaaat
    10381 attggcgggc ggggatttca aactgtccga cctgcaaaat atcgcgacct tcggcaatgt
    10441 tgtcgaacac ggttttgtcg ttttgcaaac cttcgcggct ttggtcaatc aagctcattt
    10501 tcacggtttg cccgattttc acttcgcccg aatcgggctg ctctttgccc gcaatcattt
    10561 tgaacagcgt cgatttaccc gcgccgttcg ggccgatgat gccgacaatc gcgcccgccg
    10621 gcactttgaa gctcaaatcg tcaatcagca ctttatcgcc gaacgatttg gaaacattca
    10681 caaattcaat cacttcgtta cccaaacgct cggcaacagg gataaagatt tcctgagttt
    10741 cgttgcgttt ttggtattcg tagttgctca tttcttcaaa acgcgccaaa cgcgctttgg
    10801 gcttggcttg gcggcctttg gcattttggc gcacccattc caattcctgc ttcatcgcct
    10861 tcacgcgcgc ggcttcggat ttcgcctcgt tttccaagcg tttttctttc tgctccagcc
    10921 aagacgagta attgcctttc cacggaatgc cgtgtccgcg gtcgagttcc aaaatccatt
    10981 cggcggcgtt gtcgaggaag tagcggtcgt gcgttaccgc gaccactgtg ccggggaagc
    11041 gcacgaggaa ttgctccagc cattccaccg attccgcatc caagtggttg gtcggctcgt
    11101 ccaagagcag catatcgggc ttgctcaaca aaagtttgca caaagcgacg cggcgttttt
    11161 caccgcccga aagattgccg attttggcat cccaatcagg caggcgcagc gcgtcggcag
    11221 cgatttccaa ttcgtgttcc gcaccgccgc cagttgacga acccgccgcg ataatcgctt
    11281 ccaagcggcc ttgttcttcc gccagcgcgt caaaatccgc atcgggattg gcgtactcgg
    11341 catacacttc ttccaaacgt ttctgtgcgg cagccacttc gccgaaaccg ctttccactt
    11401 cctcgcgcac ggttttttcc ggatcaagct ccggctcttg cggcagatag ccgattttga
    11461 tgtcgcccat cggcacggct tcgccctcaa attctttatc cacgcccgcc ataatccgca
    11521 gcacggtgga cttgcccgtg ccgttcaagc ccaagaggcc gattttcgcg ccggggaaga
    11581 aagaaaggga aatatcttta atgatggttt tctgcggcgg cacaaccttg ctcacgcgca
    11641 gcatagaata gacgtattgt tgggacatag ttttctcgat tcggtcaaac aaatttcaga
    11701 cggccatttt aaccgataat ttgatttaag ccagtttatc cgcgaaccgg tattgccaaa
    11761 atcgggcagg attcataaaa tccgcctatc cctttgaaat tatacagaca aaaaaataat
    11821 aatgataggg gatcgccgcg ccggcaacca tttcggattt tccaaagcaa atacttttca
    11881 aatcaaaaaa aaggatttac tttatgtcgg aatatacgcc tcaaacagca aaacaaggtt
    11941 tgcccgcgcc ggcaaaaagc acgatttgga tgttgagctt cggctatctc ggcgttcaga
    12001 cggcctttac cctgcaaagc tcgcagatga gccgcatttt tcaaacgcta ggcgcagacc
    12061 cgcacaattt gggctggttt ttcatcctgc cgccgctggc ggggatgctg gttcagccga
    12121 tagtggctac tactcagacc gcacttggaa gccgcgcttg ggcggccgcc gcctgccgta
    12181 tctgctttac ggcacgctga ttgcggtcat cgtgatgatt ttgatgccga actcgggcag
    12241 cttcggtttc ggctatgcgt cgctggcggc cttgtcgttc ggcgcgctga tgattgcgct
    12301 gttggacgtg tcgtcgaata tggcgatgca gccgtttaag atgatggtcg gcgatatggt
    12361 caacgaggag cagaaaagct acgcctacgg gattcaaagt ttcttagcga atacggacgc
    12421 ggttgtggca gcgattctgc cgtttgtgtt cgcgtatatc ggtttggcga acactgccga
    12481 gaaaggcgtt gtgccacaaa ccgtggtcgt agcattctat gtgggtgcgg cgttactgat
    12541 tattaccagt gcgttcacaa tctccaaagt caaagaatac gacccggaaa cctacgcccg
    12601 ttaccacggc atcgatgtcg ccgcgaatca ggaaaaagcc aactggttcg aactcttaaa
    12661 aaccgcgcct aaagtgtttt ggacggttac tccggtacag tttttctgct ggttcgcctt
    12721 ccggtatatg tggacttact cggcaggcgc gattgcagaa aacgtctggc acactaccga
    12781 tgcgtcttcc gtaggccatc aggaggcggg caaccggtac ggcgttttgg cggcggtgta
    12841 gtcggttgcg gcggtgattt gttcgtttat tctggcaaaa gtaccgaata aataccataa
    12901 ggcgggttat ttcggctgtt tggctttggg cgcgctcggt ttcttctcta tcttcttcat
    12961 ctacaatcaa tacgcactca tcctgtctta tatcttaatc ggcatcgctt gggcgggcat
    13021 tatcacttat ccgctgacga ttgtggccaa cgctttgtcg ggcaaacaca tggatactta
    13081 tttgggcctg tttaacggct ctgtctgtat gccgcaaatc gtcgcttcgc tgttgagttt
    13141 cgtgcttttc ccgatgctgg gcggccatca ggcaaccatg ttcttggttg caggcgcagt
    13201 cttgctgctg ggagccttct cagtctgtct gattaaagag acccacggcg gggtttgagc
    13261 gatgagcgat acccccgcta cccgcgattt cggcctgatc gacgggcggg ccgtaaccgg
    13321 ctatgtgctg tccaaccggc gtggtacgtg cgtcttcgtg ctggacttgg gcgggattgt
    13381 gcaggaattt tccgttttgg cagacggcgt gcgcgaaaac cccgtggtgt cgttcgacga
    13441 tgcggcttcc tatgcggaca atccgtttca gattaacaag cagatagggc gcgtggccgg
    13501 acgcatccgc ggtgcggcgt tcgacatcaa cggtaggact taccgcgtgg aggccaacga
    13561 aggcaggaac gcgctgcacg gcggttcgca cgggctggcc gttacccgtt tcaacgcggt
    13621 ggcggcagac ggccgacggt tatcccaacg atttggatat ttcctaccgc ttggacgagg
    13681 atggccggct taccgttacc tatcgcgcca ccgcgctcgg cgacacggtg ttcgacccga
    13741 cgctgcacat ttactggcgg ctggacgcgg gcctgcacga tgcggttctg catattccgc
    13801 agggcggaca tattccggcc gatgccgaaa aactgcccgt cttaacggtt tcagacggcc
    13861 tcgaagtatt tgatttcagc cggcccaagc cgccggatgc cgccgttgcc gccctgcgcc
    13921 gcgaaacggg ccggttttga cgacgcttac cgcgtgccgt ccgatatagg ccgtcccgcc
    13981 gccgtgttgc aagccggacg ccgccgtcgt atcagcatat acagcgaccg caacggcttg
    14041 gtcatcttta ccgccgcccc gcaggatttc gcgcggcacg atgcgggcgt ttacgacgcg
    14101 ctggcgaccg aggcgcagac gctgcccgac agcctgcatt ggcccgaatt cggcaatatc
    14161 cgtctgaaca agggtgatac tagggaggcg acgattgctt acggcatcga atccctttct
    14221 taggagcttc ctaacaccgg tttcagacga cctttttata gtggattaac a
//
LOCUS       DAXDXW010000066        13550 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXDXW010000066 DAXDXW010000000
VERSION     DAXDXW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13550
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.067"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            129..619
                     /locus_tag="ACFB64_001648"
                     /pseudo
     CDS             129..619
                     /locus_tag="ACFB64_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            656..922
                     /locus_tag="ACFB64_001649"
                     /pseudo
     CDS             656..922
                     /locus_tag="ACFB64_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1424..2047
                     /locus_tag="ACFB64_001650"
     CDS             1424..2047
                     /locus_tag="ACFB64_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC1209974.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLISPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPDEGERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGDIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            2159..3541
                     /locus_tag="ACFB64_001651"
     CDS             2159..3541
                     /locus_tag="ACFB64_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC1209975.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAIYAAS
                     LPGAEDNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PMIADVAGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTRLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMHSIWLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFAGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNRDGLQRALLQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            3545..4069
                     /gene="ssb"
                     /locus_tag="ACFB64_001652"
     CDS             3545..4069
                     /gene="ssb"
                     /locus_tag="ACFB64_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC1209976.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYDEGYGQNQEAYQRPAQQSRQPAPDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            4442..5365
                     /locus_tag="ACFB64_001653"
     CDS             4442..5365
                     /locus_tag="ACFB64_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC1209977.1"
                     /translation="MENQRPLLGFALALLAAMTWGMLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLTLGGRLPKRRDFSWHSFRLLLLGVTGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLVGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFVYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRLFKRR"
     gene            complement(5430..6818)
                     /gene="fumC"
                     /locus_tag="ACFB64_001654"
     CDS             complement(5430..6818)
                     /gene="fumC"
                     /locus_tag="ACFB64_001654"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC1209978.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYRPVHPNDHVNRAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHSLGRLNDAL
                     KGLYELALGGTAVGTGLNSHPEYAEIAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNENCAAGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNDKSLRETAVGLGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(6962..8941)
                     /gene="tkt"
                     /locus_tag="ACFB64_001655"
     CDS             complement(6962..8941)
                     /gene="tkt"
                     /locus_tag="ACFB64_001655"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC1209979.1"
                     /translation="MSQLANVIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQTAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     AYPAFEIPQEIYDAWNAKEKGAKLEAGWNELFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKAETVATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAAKAED
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGAYVISEAQGNAQAVIIATGSEVGLAVEAQK
                     VLAGQGIAVRVVSMPSTSVFDRQDAAYQAAVLPEGLPRIAVEAGHTNGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            9475..10971
                     /gene="ccoG"
                     /locus_tag="ACFB64_001656"
     CDS             9475..10971
                     /gene="ccoG"
                     /locus_tag="ACFB64_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC1209980.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKVKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPSGLI
                     RYTTESALEHEYAEKDIKKRLLRPRVAGYGAVLAVVVAAFLVGLSTRKMVEVDILKDR
                     GVMVRENAKGWLENAYSLRIINNSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETI
                     TLPVQVSTIPEYADKGSHPIEFIFQYRESGASDGKPVVLEEDATFIGE"
     gene            10974..11522
                     /locus_tag="ACFB64_001657"
     CDS             10974..11522
                     /locus_tag="ACFB64_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC1209981.1"
                     /translation="MSQNNPIKPWYKHVWPWVLMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLPPANHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(11864..13039)
                     /locus_tag="ACFB64_001658"
     CDS             complement(11864..13039)
                     /locus_tag="ACFB64_001658"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC1209982.1"
                     /translation="MQNIFDPLIIRGKSLIPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKNLGLEGEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLASAETEKVV
                     EFMSVAGLPARGIRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDQLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(13197..13313)
                     /locus_tag="ACFB64_001659"
                     /pseudo
     CDS             complement(13197..13313)
                     /locus_tag="ACFB64_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(13476..13550)
                     /locus_tag="ACFB64_001660"
     tRNA            complement(13476..13550)
                     /locus_tag="ACFB64_001660"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13515..13517),aa:Glu,seq:ttc)
BASE COUNT         2945 a         3372 c         3892 g         3341 t
ORIGIN      
        1 gcaagcgtag gtcggatact tgtatccgac aaaaccttta acattcccat cattgcaatc
       61 cattgcagca atgtccaaaa tgtcgaattc aagaatccgg cctacaaaat cattccgagc
      121 ataatactat gaaataccgt cgtttttacc gcaatggcgg cacttacttt tttacggttg
      181 taaccaataa acggcagaag attttgaccg atgatgcggt gcgtttggct ttacggcagg
      241 cggtaatggc ggtgcgcgaa cggaatccgt ttgaaatttt ggcatgggtg ttgatgcccg
      301 atcatctgca taccatatgg cggctgccgg acaatgattc tgcttattcg gaacgctggc
      361 ggcaaatcaa gcggcacagc caatatttaa tcggcggcaa tctcaggctt tggcaaaacg
      421 cttttgggaa catactatcc gcggtgaggc cgattttgcc tgccattttg attatctgca
      481 tttcaatccg gtcaaacatg gctatgtagg acaaatttcc gattggcggt tttctacgtt
      541 tcaccgttat gtcaaacagg gtatttatcc gcataattgg ggtggcggca atgcggactt
      601 ttctattgaa tacgattgaa gtaatgtcgg attcgagaat ccgacctacg gaaaactgaa
      661 agagcatcgg ctgctggacg gcattatgcg caaagcccgc cgcaaccgcc cgctgccgga
      721 ggcgcaaacc aaacgcaacc gatatttgtc gaagacccgt tatgtggtcg aacaaagctt
      781 cggtacgctg caccgtaaat tccgctatgc ccgggcggcc tatttcggac tgattaaagt
      841 gagtgcgcaa agccacctga aggcgatgtg tttgaacctg ttgaaagcgg ccaacaggct
      901 aagtgcgccc gctgccgcct aaaaggcggc ccggatgcct gattatcggg tattcaaggg
      961 ggattaaggg ggtatttggg tagagttagg agtgattggt ggcggaaaca gccgaaaatc
     1021 tgtgtcaggg tttcagctgt cgggagggaa gggaattttg caaaggtctc aagatatagt
     1081 ggattaaatt taaattagta cggcaaggcg aggcaacgcc gtaccggttt aaatttaatc
     1141 cactatattt ccgcctgctg ccgcccgaaa agcgtaatgc gctgatggtg ctgctgcaca
     1201 atccggacga cggataaccg gtgaaaacgg gaaatgcccg ggcggtgtcg tctgtgcctg
     1261 tttggacaag cgttcttgag ggcggtaaaa tcaaggtttg cccgaaaagg gcagggcgat
     1321 atgccgcgct tggattttgc gcgcggtttt tcgcgttgga agcaatcatg tctgtttgaa
     1381 tatttaatgc cgtctgaaac gtccggacgc acccccgttc cttatgaaaa aaccgaccga
     1441 taccctaccc gtcaatctgc aacgccgccg cctgctgtgt gccgccggcg cgctgttgat
     1501 cagcccgctg gcgcacgccg gcgcgcaacg tgaagaaacg cttgccgacg atgtggcttc
     1561 cgtgatgagg agttctgtcg gcagcgtcaa tccgccgagg ctggtgttcg acaatccgaa
     1621 agagggcgaa cgttggttgt ccgcgatgtc ggcacgtttg gcaagattcg tccccgacga
     1681 gggggagcgg cgcaggctgc tggtcaatat ccaatacgaa agcagccggg ccggtttgga
     1741 tacgcagatt gtgttggggc tgattgaagt ggaaagcgcg ttccggcagt atgcgataag
     1801 cggggtcggc gcgcgcggtc tgatgcaggt tatgccgttt tggaaaaact acatcggcaa
     1861 accggcgcac aacctgttcg atatccgtac caacctgcgt tacggctgca ccatcctgcg
     1921 ccattaccga aaccttgaaa agggcgacat cgtccgcgca cttgcccgct tcaacggcag
     1981 cctcggcagc aataaatatc cgaacgccgt tttgggcgcg tggcgcaacc gctggcagtg
     2041 gcgttgattt tgaacccgtg ccgcaaccga aatacggcga atcctgtata atccgaaaat
     2101 ctgttcactg gaagttcaga cggcattgca actgttgatg ccgtctgaaa aattatttat
     2161 ggcaagagac aaccgcatcc aaatgtttcc gcacgaatgg cgcgccagta cgacgctttc
     2221 cggcgtgtac gcgctgcgta tgctgggtat gttcctcgtg ctgcccgttt tggcaatata
     2281 tgccgcctcg ctgcccggtg cggaagacaa taaaacgctg gtcgggctgg caatgggcat
     2341 ttacgggctg acgcaggcat tgctgcaact gcctttgggt atcgcttccg acaagttcgg
     2401 gcgcaagaaa accatttatg tcgggctggt cgtgtttgcc gccggcagct tccttgccgc
     2461 cgccgccgat acgctgccca tgctggttgc cgcgcgcgcc atacagggcg cgggggcggt
     2521 cagtgctgcc gtaaccgcgc tgcttgccga cttgacgcgc gacggcgtgc gtacccgcgc
     2581 gatggcgatg atcggtttga gtatcggtct gacgttttcg gtcagcctgg tggttgcgcc
     2641 gatgattgcc gatgtggccg gcgttcgcgg actgtttatg ctgaccggca ttctgaccgt
     2701 catcagcatc ggcgtagtgg cgtggatgac tcctgatccc gaagtttcca agctgcatga
     2761 ggacacgcag gcgcagcctt cgcgcatagg agaagttttg aaaaaccgta ggctgctgac
     2821 gctcgatttc ggcattttcg ccctgcacgc cgcgcaaatg gcattgttta ccgcgctgcc
     2881 tttcgcgatg acccggctcg gtttggaaaa aatccagcat tggaaagtat atctgccctc
     2941 gaccattacg ggcttggtgg tgatggttcc gctgattatc gtcggcgaaa cgcgcaacaa
     3001 gctcaaacag gtttttgttt tgggcatcgt ctgcatcgcg gcggcgcagc ttggtttgct
     3061 gtccggaatg cactcgattt ggctgattac cgcttatctg gttgtttact ttatcggttt
     3121 taatgtgttg gaagcgagcc tgccgtctat ggtttccaaa atcgcgccgt ccgacctgaa
     3181 gggtacggcg atgggcgtgt acaacacgat gcagtcgctc ggactgtttg ccggcggcgc
     3241 ggcaggaggc ttgctgtttc aaaaatacgg ctttgccggc gtgtttgcct tttgcagcat
     3301 attgatgctg ctgtggctgg tgattgccgt tttatcgccc gcgcccaagc ccgtcaaaaa
     3361 cctcagttac cctgtcggcg gcgtgtggca gggcaatcgg gacggtttgc aacgcgccct
     3421 gttgcagttg gaaggcgtgg aagacatcgg tttcagtttc gacgggcaga ccgtctatct
     3481 taaagtgttg cagaagggtt tcgatcaggc tgccgctgaa aaaatcatta caggagttta
     3541 aaaaatgtca ttgaacaaag tcatccttat cggccgcctc ggacgcgatc ccgaagtgcg
     3601 ctatatgccc aacggcgagg cagtgtgtaa tttcagcgtc gccaccagcg aaacttggaa
     3661 cgaccgcaac ggccagcgcg tagagcgcac cgaatggcac aacatcacga tgtaccgcaa
     3721 actggcggaa attgccgggc aatacctcaa aaaaggcggg ctggtttatc tggaaggcag
     3781 aatccaaagc cgcaaatacc aaggcaaaga cggcatcgaa cgcaccgctt acgatattgt
     3841 cgccaatgaa atgaaaatgt tgggcgggcg caatgaaaac agcggcggcg cgccttacga
     3901 tgaaggttac ggtcagaatc aggaggctta ccaacgcccc gctcagcaaa gtcggcagcc
     3961 cgctcccgac gcgccgtccc atccccaaga agcacccgcc gcgccgcgcc gccaacccgt
     4021 gccggccgcc gccccggtcg aggacattga cgacgacatc ccgttctgaa ttttacggcc
     4081 ggacatcctc gcggagggaa gtcataaagg acggaaaaac cttaaacctt acggggcaag
     4141 gtttctcttt tttgcatttg ggccggcttg ttgccgcccc ctgcgttggg cgtaacgttg
     4201 cgattgcgcc gttcgggcgt tttgaggcaa gcgatgcctg ccgcactccc ccgctgtttt
     4261 cagacggcat tttaaaattg aggcggtgtt ttcaaatagt gcgaagcaat agtcgggctt
     4321 cagccagttt gtttgaaatc aaagggttgg aatcaaacgc caaacgcttg aaccatcatt
     4381 gcccgatgcg gcaaaccggc atcgtccggc aatacggttg tttaaaaata gggaaatcag
     4441 gatggaaaac caaaggccgc tcctaggctt cgcgttggca cttttggcgg cgatgacgtg
     4501 ggggatgctg ccgattgccg tgcggcaggt attgaagttt gtcgatgcgc cgacgctggt
     4561 gtgggtgcgt tttacagtgg cggcggcggt attgtttgtt ttgctgacat tgggcgggcg
     4621 gctgccgaag cggcgggatt tttcttggca ttcattcagg ctgctgctgc tcggcgtgac
     4681 gggcatttcg gcaaactttg tgctgattgc ccaagggctg cattatattt cgccgaccac
     4741 gacgcaggtt ttgtggcaga tttcgccgtt tacgatgatt gttgtcggcg tgttggtgtt
     4801 taaagaccgg atgactgccg cgcagaaaat cggtttggtt ttgctgcttg tcggtttgct
     4861 tatgtttttt aacgacaaat tcggcgagtt gtcgggtttg ggcgcgtatg cgaagggcgt
     4921 gttgctgtgt gcggcaggca gtatggcctg ggtgtgttat gccgtggcgc aaaagctgct
     4981 gtcggcgcaa ttcggtccgc aacagattct gctgttgatt tatgcggcaa gtgccgccgt
     5041 gttcctgccg tttgccgaac cggcacacat cggaagtttg gacggtacgt tggcgtgggt
     5101 ttgttttgtg tattgctgct tgaatacgtt aatcggttac ggctcgttcg gcgaggcgtt
     5161 gaaacattgg gaggcttcca aagtcagcgc ggtaacaacc ttgctccccg tgtttaccgt
     5221 aatattttct ttgctcgggc attatgtgat gcctgatact tttgccgcgc cggatatgaa
     5281 cggtttgggt tatgtcggcg cactcgtcgt ggtcgggggt gcggttacgg cggcggtggg
     5341 ggacaggctg ttcaaacgcc gctagttcgc aggcaacgga aaatgccgtc tgaacgaggc
     5401 ttcagacggc attttatttg agggaaggat tagcgcggat gaaccatatc ggcagggacg
     5461 accagttcgt caaactcttc gcccgtcagc aagcccaacc caacggcagt ttcgcgcagc
     5521 gatttatcgt ttttgtaggc ggttttggcg actttggcgg cgttttcgta gccgattttg
     5581 cggtttaacg cagtaaccag catcagggaa tggtgcagga aatagtcgat tttttccggt
     5641 acgggttcga tgccggcggc gcagttttcg ttgaagctgt tgcacgcgtc gcccaagagg
     5701 cggatggatt gcaagaggtt gtaggcgata acgggcatat agacgttcag ctcgaaattg
     5761 cccgacgcgc ccgccatacc gatggtaacg tcgttgccga acacttggca gcacaccatc
     5821 gtcatcgctt cgcattgggt cgggttgact ttgcccggca taatggacga acccggttcg
     5881 ttttcgggga ttttgatttc gcccaagccg cagcgcgggc cgcttgccag ccagcggatg
     5941 tcgttggcga ttttgttcag gcttgccgcc agcgttttca atgcgcccga agcggcaacg
     6001 gcggcatcgc gtccgcccaa ggcttcaaat ttgttcggcg cgctgacaaa cggcaggccg
     6061 gacagttcgg cgagtttggc ggcggctatt tcagcgtatt cgggatggct gttcaaaccc
     6121 gtaccgaccg ccgttccacc caaggcgagt tcgtacaaac ctttgagcgc gtcgttcaga
     6181 cggcctaagc tgtggtcgag ttgggaaacg tagccggaaa attcttgtcc caaagtcagc
     6241 ggcgtcgcat cctgcaagtg ggtacggccg attttgacga tgggggcgaa ggcttgggct
     6301 tttttgtcca atgtatcgcg caaggctttt accgccggaa taaggtggcg gttgatttca
     6361 atcgcggcgg cgacgtggat ggcggtcggg aacgcgtcgt tggtcgattg cgcgcggttc
     6421 acatggtcgt tggggtggac gggccggtac gccgccaaac ccgtacctgc aatttcgttg
     6481 gcgcggtttg ccagcacttc gttcatgttc atattggact gcgtaccgga accggtctgc
     6541 caaaccacta aggggaactg cccgtcgagc ctgccgttca gtacatcatc cgccgcctgc
     6601 gtaatcaaat ccgcctgttc gggcttaatc ctgccgaggg aaacattggt cgcggcggcg
     6661 gcttttttca ccaacgccaa agcgtaaatc agcggctgcg gcagggtttc gccgccgatt
     6721 ttgaaattgt tgcggctgcg ctgggtctgc gcgccccaat aggcttcgga cgggacttcg
     6781 acattgccca tcgtgtcgtg ttcggtgcgg gtgttcatac gtttctcctt ttgaaatgtg
     6841 aataagagtg attcgcaaat attataatgg agattggcgg aatgaggaag catcaaggcg
     6901 ggagggcaaa aaaatgccgt ctgaaaagca ttcagacggc atttgatgcg gcggctgagg
     6961 tttacagcac ggatttcacc gtatcaacca cattgtccac ggtaaagccg aatgctttga
     7021 acagcagatc ggcaggggcg gattcgccga agcggttgat gccgacgact gcgccgttca
     7081 gtccgacata tttgtaccag ccgttggtgt gtccggcttc tacggcgatg cgcggcaggc
     7141 cttccggtag gacggcggct tgataggcgg cgtcttggcg gtcgaagacg ctggtggacg
     7201 gcatggaaac gacgcgcacg gcaatacctt gtcctgccaa tactttttgc gcttctacag
     7261 ccaatcctac ctcggaaccg gtggcaatga tgacggcttg ggcgttgcct tgggcttcac
     7321 tgattacgta agcaccgcgt ttgatgtcgt tcagttgttg ctcgctgcgt gcttggaatt
     7381 tcaggttttg acggctgaaa atcaggcagg acgggtggtc ttcggctttt gccgcttctg
     7441 cccaagcgac cagagattct gcggtgtcgc acggccgcca tacgtccata ttcggaatca
     7501 ggcgcagggt ggcggtttgc tcgataggct ggtgggttgg gccgtcctcg cccaaaccga
     7561 tggaatcgtg ggtgaatacg aagacggggt tgattttcat cagtgccgcc atgcgtaagg
     7621 cgttgcgttc gtattcgctg aacatcagga aagtcgcgcc gaagggtttt acaccgccgt
     7681 gcaataccaa accgttcata atcgcaccca taccgaactc gcgcacgccg tagtggatgt
     7741 agttgccgcc tttgtcgcgg gtaacggaga cgctgtttga ccagtcggtc aggttggacg
     7801 gggtcaggtc ggcagaaccg cccaccagtt cgggcagctc ttttgccaag atttcgatgc
     7861 tgttttggct ggctttgcgg gtggcgacgg tttccgcttt ggcgcacact tctttcaatg
     7921 ccgtctgaac gtactcgtca aagttttccg gcagcttttt atccatgcgg cgcacgaatt
     7981 ctgcggcttc ggcaggatat ttggcttgat attgcgcgaa cagttcgttc catccggctt
     8041 ccagtttcgc gcctttttct ttggcattcc acgcatcgta aatttcttgc gggatttcaa
     8101 acgcaggata agcccaaccc aaatgtttgc gcgtggcttc gatttcgtcc gcacccaaag
     8161 gcgcgccgtg ggttttgtgg ctgccttctt tgttggcgct gcctttgccg attaaggttt
     8221 tgcagcagat gatggacggc ttgccggttt cggcgcgggc ggcttcgatg gccgtctgaa
     8281 tggcggcggt gtcgtggccg tttacgttgg gaacgacgtg ccagccgtag ctttcaaagc
     8341 gttgcgggat gttttcggta aaccagccgt ccactttgcc gtcgatggag atattgttat
     8401 cgtcatacaa aacaatcagt ttgcccaagc ccaaggtgcc ggcgagtgag caggcttcgt
     8461 gcgatacgcc ttccatcagg cagccgtcgc ccataaagac gtaagtgtag tgatcgacga
     8521 tgttcaaacc gtctttatta aattcggcgg caaggatttt ttctgccaat gccatgccca
     8581 ctgcgttggc aataccttgc cccaacgggc cggtcgtggt ttccacgccg tcggtgtagc
     8641 cgtattcggg atggccgggg gttttgctgt gcagctggcg gaagtttttc agatcttcaa
     8701 tgctcaggtt gtagccggtc aggtgcagca ggctgtacaa cagcatagac gcatgaccgt
     8761 tggagaggac gaagcggtcg cggttgtaga atttgggatt ggcgggattg tgattgagga
     8821 atttcgtcca caatgtttcc gccatttccg ccatacccat aggcgcgccg gggtggccgg
     8881 aattggcttt ttgaacggca tcggccgaga ggaagcggat tacgtttgcc agttgagaca
     8941 ttttgtattt tccttgctgg tgtttcggat aagtggataa tcggaaggcg ttgattatcg
     9001 cccgattcgc ttatgctttc aagaaaaggg cggacgcggg ggaaggcggc ggcagggggt
     9061 gggcggtggg gaaggatttt cgatttgcgg gcgaagcctg ccattattcc ttttgaaata
     9121 aaacgttata gattgtgtgc cggattgttg atagcgtttg tttatgagct tgcgccggcg
     9181 gttctgccga taagggggtg tctgtttttt tagtctttgt tttttaagag tattttaatt
     9241 ttgttcgggc ggtgcggtaa aatcagggcg ttttgatgcg gatgggaaat gtattcgccc
     9301 aatacatccg gacggcgcat aaagttgtac aatagcggga atatattttg ggaaaagcgg
     9361 attttctcaa aacttgaaac acaatgcctt aaaaaaacaa gaaaaatgac gtctgaaaag
     9421 caaaacggcg gcggtaacgg gcaaaccatc aatcaaaaat ctaaggaatg cagaatgacc
     9481 acggaaaacc aagccggcag tccggcatcc ggaatcggca cgtccgaaca aacaaaagcc
     9541 gcgccgaaag tgaaaaaaac gttcgatccg cgtgccagcg tgattcaaat ccatcccgaa
     9601 ggcgaacgca tccatcccaa aaaggcggaa ggacggttcg ccaaactgcg tatcgccgcc
     9661 gtattggcga cccagtttgt gttttacgtc attccgtggt tcaattggag cggaaggcag
     9721 gccgtcgttt tcaatatccc cgaacggcat ttcttcattt tcggattgtc gttgggggtg
     9781 ggcgatttga tttaccttgc cctgctgctg atgatttgcg ccttcgggct gttttggtgg
     9841 acgacgattg ccggccgttt gtggtgcggc tattcctgcc cgcaaacggt ttacaccgaa
     9901 attatgctgt ggatcgacaa cctggtcgaa ggcgatagaa acaaacgcct gaagctggaa
     9961 aaatcgccgt ggaatttcac caaaatccgc atcaaagcca ccaaatacct gttgattttc
    10021 cttgtctgcg cgtggacggg catcacgttt gccggctggt ttgtccccat ccgccagttt
    10081 gttcccgatt tattcaccgg agcagcaggc ggcggcgcga tgtttgccgc agcgttttac
    10141 ggctttatga ccttcttctt cgcccatatt atgcgcgaaa aagtgtgcct gcatatgtgt
    10201 ccgtacgcac gtttccaaag cgcgatgttc gataaggaca cgctgattgt ttcttatgac
    10261 gcggagcgcg gcgaaccgcg cggcgcccgc aagaaaacgg tcaacaagga agaggcgggt
    10321 ttgggcgact gcatcaactg tgcgatgtgc gtccaagtct gccccgtcgg catcgacatc
    10381 cgcaacggcc tgcaatacca atgtatcggc tgcgccgcct gtatcgacgc gtgcgatgag
    10441 attatggaca aaatgggtta tccgagcggt ttgatacgct atacgaccga aagcgcgctg
    10501 gaacacgaat atgccgaaaa agacattaaa aaacggctgc tcagaccgcg cgtggcaggt
    10561 tacggcgcgg tgttggcagt ggttgtcgcc gccttcctgg tcggtttgtc cacgcgcaaa
    10621 atggtcgaag tcgatattct gaaagaccgt ggcgtaatgg tgcgcgaaaa cgccaaaggt
    10681 tggctggaaa acgcatatag cctgcgtatc atcaacaaca gcgaaaaaga acagctgatt
    10741 accgcaagtg tcaaaggatt tgacgaaatc gccctgaccg ggctgcctga aggcggtata
    10801 aaagtggctc cgcgcgaaac aataaccctt cccgtccaag tgtccaccat tccggaatac
    10861 gcggacaaag gcagtcatcc tatcgagttt atcttccaat accgggaaag cggtgcatca
    10921 gacggcaagc cggttgtttt ggaagaagac gcaaccttta tcggagaata accgtgtctc
    10981 aaaacaatcc aatcaagcct tggtacaaac acgtctggcc gtgggtcttg atggcggggc
    11041 cgatttttgt cgtcatcgcc agcgtcgcta tgttttttgt cgcgcagcag cacgcgacag
    11101 atttggttac ggacgattat tataaggatg gcaagcatat cgacatccag cttcatcggg
    11161 atgaagaagc cgtcagacgg catatcgggg tgcaggtcct catttctccc gatatgaatg
    11221 cggcaaaagt gtttgtcggc ggcgagtttg acggcaaaca gcctttgaac ctgctgctga
    11281 tgcacccgac ccgcaaggcg gacgatcaaa ccgtcgccct caagcccgtc ggcagcgcgc
    11341 agaacggcag ggcggaatat gaggcggtgt tcaaaaccct tccgccggcc aaccactggt
    11401 atgtgcgcgt ggaggacgcg gcaggcgtgt ggcgcgtcga gaacaaatgg attaccagcc
    11461 agggcaatgc ggtcgatttg accccgatgg acaaactttt caataatgca ggaagcaaat
    11521 aaaaaagccg tgttttcatt gcattggttt gttatatgca atgtcgggca ggcattgttt
    11581 gattattcgg cgtgatggtc tgatttgtcg gacgaaaata aatgtatctc atcccgttta
    11641 ttttttaaca tcattccgaa aggcagcctg aaaaatgccg tctgaaaggc ttcagacggc
    11701 atttgtgttg tatgtccgac ataatgcgga aggcattttg ttcatataaa aacgttttta
    11761 tttctctccg ttttttcgac actgttccgg aacacaacct gaaaaacgcc gtctgaaagc
    11821 ccttcggacg gcattttgtt tgcaaatcaa atcctacctg atgtcagcgt ccgagcgttg
    11881 caacaggttc gctccccgtc agcagatatt gtatcgtttc gcggacggtg cggatggcat
    11941 taccgaaggg aagctggtct tttcccctga aaaacaaccc cttatcaact tctccacgga
    12001 atgcggcagc aagctggata tcaatacaga attgccctgc tttggaaagc ccgtcgcgca
    12061 gaccgcagct ggtcaggcag tttaaacctt gggtacagcg gcgcgggtcg gctttggcgt
    12121 ttgcctgaag tttgccttcg cgcctgatat agctgtctag gaatttggtg cggataccgc
    12181 gcgccggcaa accggcaaca gacataaatt cgactacttt ttcagtttcc gcactggcga
    12241 gcgttttttt aaagttgagg tgtgcatctc cttcttcggt aacggcaaaa gccgtaccga
    12301 tttgaacggc ggaggctccc cagttcttta gggcggtttt gactttttca aaatttgcca
    12361 tgcctcccgc aagaataagc gggatttttt ctccctccag ccccaaattt ttaaacactt
    12421 caaacgtttc ctcaatcacg cgtttgaagt cgaatttggc atcgtttacg ccttcaacgg
    12481 ttgatgcacc caaatgtccg gccgcatggg caggatgttc gactacaatc gcatcgggca
    12541 atatgccttt tttcatccaa cgtttcaaga cgatattaat accgcgcgat tcggacagaa
    12601 tcggcagcag cgcgacatcc ttatgatagc cctcggtcat ttccggcagg tctaaaggca
    12661 ggccggcacc catcacgacc gcatccgccc ctgattcgca agcctggcgg acatacgcgg
    12721 cgtggtcttt gaccgccttc atcacgttga ccgcaatcag tccttttccc tctgaagcac
    12781 ttttggcttt ttggatttcc ctgtctaatg cggtacagtt caaagacgta tatttctctt
    12841 cactcggatt gatttgggat tcggcgagca ggtcttcgtg aaggtggcgc aaatctacac
    12901 tggcaatcgt tccgatgccg ttttcacgcg ccaccgcgct ggataaaccc gatgcggaaa
    12961 caccgacccc cataccgcct tgcacgatgg gaataaggga ttttccacga ataatcaaag
    13021 ggtcaaaaat attctgcatc agtttctccg aatatacgaa tcagtcaggc tcatgaaaaa
    13081 tggttttaat tgttttagaa ccattactca aatttggtat tatactctaa atattgcagt
    13141 ggtaaacaga aaagcagaag gagctttgga atatgctgtt atatctgtac caatcggaca
    13201 aaatatagtg gattaaattt caaccggtac ggcgttgcct cgccttgccg tactatttgt
    13261 actgtctggg gcttcgtcgc attgtcctga tttaaattta atccactata ttgaacggac
    13321 accgcatccg gttatgggat atcggtttgc atggcaaaag tttgaatatg attcgattac
    13381 gtctgagatt ctgatgaagt gggtaggtaa agatatggta acgaattgcc gaggcaataa
    13441 aaatccccga gggattattt cggggatttg gaatctggca cgcccacggg gaatcgaacc
    13501 ccggttaccg ccgtgaaagg gcaatgtcct aaccgctaga cgatgggcgc
//
LOCUS       DAXDXW010000067        13445 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXDXW010000067 DAXDXW010000000
VERSION     DAXDXW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13445
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.068"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(263..1198)
                     /gene="hemC"
                     /locus_tag="ACFB64_001661"
     CDS             complement(263..1198)
                     /gene="hemC"
                     /locus_tag="ACFB64_001661"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC1209983.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDRILDRTLSKVGGKGLFVKELEQSLQDGRADLAVHSIKDVPMDLPEGFALAAISERA
                     NPFDAFVSNRYARLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDERIRMILSESDSLPAAGQGALGIEIATHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLILRGLVGHPDGSIVLQADA
                     QAPAGYADALGRAVAKKLADDGAQELIGAVLNTEN"
     gene            1317..1832
                     /gene="ybeY"
                     /locus_tag="ACFB64_001662"
     CDS             1317..1832
                     /gene="ybeY"
                     /locus_tag="ACFB64_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC1209984.1"
                     /translation="MKRAKKYPFLTLQRQRFHLNFENVSSAAGIPAERDFYRWAWSAL
                     KNEYLRADIGLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSERLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGTLHLMGYDHIKDDEAEIMEAEEIRLMRAA
                     GYPNPYREDGH"
     gene            1834..2658
                     /locus_tag="ACFB64_001663"
     CDS             1834..2658
                     /locus_tag="ACFB64_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC1209985.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLTRLEKVLDFAELEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDESADNIHSVSAERWRIHAATEIEDINAFF
                     GTEYGSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(2801..4180)
                     /gene="nhaC"
                     /locus_tag="ACFB64_001664"
     CDS             complement(2801..4180)
                     /gene="nhaC"
                     /locus_tag="ACFB64_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC1209986.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQAGMIGALNQGMGAVYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTACATVGVAFMGMAAAFQADMAMTAGAIV
                     SGVFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAVRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(4470..5693)
                     /locus_tag="ACFB64_001665"
     CDS             complement(4470..5693)
                     /locus_tag="ACFB64_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC1209987.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVIQGWAADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            6130..6543
                     /locus_tag="ACFB64_001666"
     CDS             6130..6543
                     /locus_tag="ACFB64_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC1209988.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVRNFPKHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLIRRFGIWLLAFAWLPVVGDALPLT
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            6590..7219
                     /gene="nth"
                     /locus_tag="ACFB64_001667"
     CDS             6590..7219
                     /gene="nth"
                     /locus_tag="ACFB64_001667"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC1209989.1"
                     /translation="MNRQIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKS"
     gene            7357..8856
                     /locus_tag="ACFB64_001668"
     CDS             7357..8856
                     /locus_tag="ACFB64_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC1209990.1"
                     /translation="MFKKYQYFALAALCAALLAGCEKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFAQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKNGYILTNTHVVAGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKNLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKASGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKAKLGN
                     AAEHTGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGKHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            9059..10579
                     /locus_tag="ACFB64_001669"
     CDS             9059..10579
                     /locus_tag="ACFB64_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC1209991.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWADGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TASPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAASDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTFGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFSTTGIVLAVLIFLLKDKKRADV"
     gene            10636..11721
                     /gene="nagZ"
                     /locus_tag="ACFB64_001670"
     CDS             10636..11721
                     /gene="nagZ"
                     /locus_tag="ACFB64_001670"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC1209992.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAIGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDSEGAET
                     AETHAEHIGWVLATELSACGIDLSFTPVLDLDWGNCAVIGNRSFHRNPEAVARLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLAPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIILVCNRPDLVDELRDGFTIPDNQDLAGRWQYMENSLGHEAVQAVIQTTG
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            11946..12022
                     /locus_tag="ACFB64_001671"
     tRNA            11946..12022
                     /locus_tag="ACFB64_001671"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11980..11982,aa:Pro,seq:cgg)
     gene            complement(12358..12996)
                     /locus_tag="ACFB64_001672"
     CDS             complement(12358..12996)
                     /locus_tag="ACFB64_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC1209993.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGRNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLKPAPPQIDYVATSPKACYAAKYETNCIAEADEVRIVADGDVLAFCENMERK
                     IRARHYYLSPCEQNGVMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(12993..13361)
                     /locus_tag="ACFB64_001673"
     CDS             complement(12993..13361)
                     /locus_tag="ACFB64_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC1209994.1"
                     /translation="MKLLSTILVLLVAAEHFYIAWLEMTQIPGEKAAEMFKLPYEFME
                     QKRVQTLFGNQGLYNGFLAVGLLWAQFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAVLAV"
BASE COUNT         3378 a         3833 c         3378 g         2856 t
ORIGIN      
        1 aaaaaatacg aatatcatca ttctcaaatt caaaatttga acgccacagt attatccgaa
       61 gccacaaaaa cctttccgcc gtcattcccg cgcaggcggg aatctggaaa cccaatgctg
      121 caagaattga ttaaaaacaa ctgaaaccga gcaaactgga ttcccgcctg cgcgggaatg
      181 acgaaataag gtaagtagaa cttattaaat aaattaatta aaaacaataa cttatattat
      241 tttctttaaa ttttcgataa aatcaatttt ccgtattcaa tactgctcca atcaattcct
      301 gcgcaccgtc gtccgccagt tttttggcaa ccgcacgtcc aagcgcatcg gcatatccgg
      361 caggggcttg cgcgtccgcc tgcaacacaa tcgatccgtc ggggtgcccg accaatccgc
      421 gcaagatcag cagcccgttt tcttcagtgc aatacgcggc cagcggcact tggcagcttc
      481 cgcccaaagc gcgcgccaaa gcgcgttcgg cggtaacgca ggcgtgtgtg gtatcgtggt
      541 ttaacggctt caagacttcg tacaaatctt cgcgatgcgt ggcaatttca atacccaatg
      601 cgccttgtcc ggcggcaggc aggctgtcgg attccgacaa aatcatgcgg atgcgttcat
      661 ccaattccag acgctgcaac ccggcggcag ccaagataat tgcatcgtat tcgccgttgt
      721 cgagtttgga caaacgggtt tgcacattgc cgcgcaaagg tttgataagc aaatgcggat
      781 agcgcgcgcg caactgggct tcacggcgca ggctggatgt gccgacaacc gcgccttcgg
      841 gcatttcttc caaacgcgcg tatcggttgg acacaaacgc gtcaaacgga ttggcgcgtt
      901 cgctgatggc ggcaagggcg aagccttcgg gcaaatccat cggcacgtcc ttaatcgaat
      961 gcaccgccaa atcggcgcgc ccgtcttgaa gggattgttc caactctttg acaaacaagc
     1021 ctttcccgcc gacttttgac aaagttctgt ccaaaatccg gtcgccgcgc gtggtcatgc
     1081 ccaaaatttc gacttcgcaa tcgggataca gcgccttcag acggccttgg atatgtttcg
     1141 cctgccacat ggcaagcagg ctttcgcggc tggcgataac aagttttttc gggttcatgt
     1201 tcaatgcgtt caaagaatgg aaacggcgtg gagtctatca tattttgaac gtttcttcag
     1261 acggcaccgt caggtctatg ttatatttac gcccacaaaa cacgaaaccc gccacaatga
     1321 aacgcgccaa aaaataccct ttcttaacgt tgcagcggca acgtttccat ttgaactttg
     1381 aaaacgtctc ttccgccgcc ggcatccccg ccgaacgcga tttttaccgc tgggcgtggt
     1441 ctgccttgaa aaacgaatac ctccgcgccg acatcggttt gattcttttg gacgaagaag
     1501 aagcccgcgc ctacaaccgc gactaccggg gcaaagatta tgccaccaat gtattgagtt
     1561 tcgcgctcaa cgaaggcgaa atcctgccct gccaagtttc ggaaagactg tacggcgatt
     1621 tgattatctg cccgcaagtc gttttaaaag aagccgccga acagggcaaa acgcctgaac
     1681 ggcattttgc ccacttaacc atacacggca cgctgcacct gatgggatac gaccacatca
     1741 aagacgatga ggccgaaata atggaagccg aagaaatccg cctgatgcgg gcggcaggct
     1801 atcccaaccc ctaccgagag gacggacatt aaaatggacg gcgcacaacc gaaaacaaat
     1861 ttttttgaac gcctgattgc ccgactcgcc cgcgaacccg attccgccga agacgtatta
     1921 aacctgcttc ggcaggcgca cgaacaggaa gtttttgatg ccgacacact gacccggctg
     1981 gaaaaagtat tggactttgc cgagctggaa gtgcgcgatg cgatgattac gcgcagccgc
     2041 atgaacgtat tgaaagaaaa cgacagcatc gaacgcatca ccgcctacgt catcgatacc
     2101 gcccattcgc gcttccccgt catcggcgaa gacaaagacg aagttttggg cattttgcac
     2161 gccaaagacc tgctcaaata tatgttcaac cccgagcagt tccacctgaa atccgtcttg
     2221 cgccctgccg ttttcgtgcc cgaaggcaaa tctttgaccg cccttttaaa agagttccgc
     2281 gaacagcgca accatatggc aatcgtcatc gacgaatacg gcggcacgtc gggtttggtc
     2341 acctttgaag acatcatcga gcaaatcgtc ggtgacatcg aagacgagtt tgacgaagac
     2401 gaaagcgccg acaacatcca ctccgtttcc gccgaacgct ggcgcatcca cgcggctacc
     2461 gaaatcgaag acatcaacgc ctttttcggt acggaatacg gcagcgaaga agccgacacc
     2521 atcggcggct tggtcattca ggaattggga cacctgcccg tgcgcggcga aaaagtcctt
     2581 atcggcggtt tgcagttcac cgtcgcccgc gccgacaacc gccgcctgca cacgctgatg
     2641 gcgacccgcg tgaagtaagc agagcctgcc cgcaccgccg tttctgcaca gtttaggatg
     2701 acggtacggt cgttttctgt ttcaatccgc cccatccgcc aaacataaaa atgccgtctg
     2761 aaaccggaaa acaggttcag acggcatttc gcttatccgc ttattttttg ctcaaagtca
     2821 gccccgtcca gccgaataac agggttaaag ccaaactcaa atagcagaaa aaggcataag
     2881 gcagatattc ccaaacgggt acgccaaggg cgtggctgat aaatacgccg cacacgctcc
     2941 acggcacgag cgggttaatc accgtccccg catcttccag agtccgcgac aggttgcgcg
     3001 aatgcaggcc gagtttgtcg taaacgggtt tgaacgtttc tcccgaaagc aggatgctca
     3061 aatattgctc tccaatcagg aaattgaccc cgaccgaagt catggcaacg ctgaacgtcg
     3121 cgcgtccggc attcgtcaag aaggtacgga cggcctccag cagggaagga atcacaccga
     3181 gcgcaaacag cagcccgccc aaactcatac cgaggataac gatggtctgc gtaaagaaca
     3241 tactctccaa gccgccgcgc gaaatcagtt tggcaatgtc tttaaacgct tcgccttcga
     3301 gtttgtagcc gccataaaac cacgcgccga gctgacgcag atcgggcgtg ctgtgcagat
     3361 acgttacggc aacggcggca atgacggtaa agagcatggc gaccacggca ttgacgcgca
     3421 tcaatgccaa aacgaccaac agtgcaaacg gaatcagcga atagccgtgc accaatcccg
     3481 tggcttcaag ctggctgcgg aaggattcga cgctgttcaa atcctgcgcg gcgacgctgg
     3541 gaagaagcca aagcatcagt gccgcgctga taagccacgc ggggatggtg gtgtacatca
     3601 tgtttttgat gtgttcaaac aggtcgatac cgacgatgga cgcggaaatg cccgtggtgt
     3661 cggaaagcgg ggacatttta tcgccgaaaa acacaccgga aacaatcgcg cccgccgtca
     3721 tcgccatatc ggcctgaaac gccgccgcca tccccataaa ggcaacgccg acagtggcgc
     3781 aggcggtcag gctgctgccg atggacacgc cgatgacgga acacagcgcg aaggcggaaa
     3841 aataaaaata agtcggggaa atcagcccga aaccgtaata catcagcgtc ggaatcgcgc
     3901 cgctcatcat cagcgcgctg accatcagcc cgatgaagaa aaacaggtaa accgcgccca
     3961 taccctgatt caacgcgcct atcatccctg cctgcatatc gttgtatttc aaaccgcgcg
     4021 ccaagccgta caaaatcagc acgacgatgg cggcaataat ggacatatgc ggcagccact
     4081 ccaatgaaat gatggtatag cccattgcgg caatcagagc gacgacgacg gcaagtgcct
     4141 caccgcgcgg catatcgagt aaggatttga aagcaaacat gattctttgc ccgatttgat
     4201 ataaagttaa cggattctaa cacaccatgc cgaatctgcc cgacgttttc agacggcatt
     4261 ttagcgtttt acaaaagccg ggcagaaaca aaacggcaat tttctcgatt taaattaatt
     4321 tttttgaaat ataattttta tttttttatt tgttggcata taagactttt atggattgaa
     4381 gtattaaaac ctcggcacac acgccgcaaa caccggtacg caaaaatgcc gtctgaaaga
     4441 tgttcagacg gcatttctca agaaaaagat tatttgtccg ccctatatcc gtacaccgca
     4501 aaaaacacga tatacaggta acagataacg gaaacgacaa acgaagacat cagggtgtaa
     4561 gtatccgccg cccaaccttg aattaccggc acgaccgcac cgccgacaat cgcggtacac
     4621 agcacaccgg aagccgcgtt ggtgaatttg cccaaccctt tggttgccaa agagaaaatc
     4681 gtcgggaaca taatcgagtt gaaaaaaccg atggcaagca gcgaccacat cgccacatcc
     4741 gcattgccgc tgcccgtcgc catcgcgacg gcaagcagca cgaccgcagc cgatgcgtta
     4801 aacgccaaat aacggttggg cgcgaatttc gccattaccg ccgaaccgag gaaacgtccg
     4861 accatcgctc cgccccaata aaacgacagg taatgcgcgg cagaagcatg atccagccct
     4921 ttcagataac ccaacacatt gaccatcagc gaaccgatag acacctccgc accgacgtag
     4981 cagaaaatgc ctgccgtacc gaacacgaga tgtttgtatt gccatacgcc ggttttgccg
     5041 tcgtggttgt gcgcgctttc ctcggcggca attttgcgcg cgtcgggcag ccggatcatt
     5101 ttcacgaaaa cggcaaggat aatcagcagc cccgccagtc ccaaataggg aatctgtacg
     5161 gaagaaatct gttccgcctt gctgacggtt tgggttgcgt ccgccaaaat caggaacgcg
     5221 ccgatttgag gcgcaatggt cgtacccaaa gcgttaaacg cctgaaccag cgtcagcgtt
     5281 gccgattcct tgccgggttt cgccagcagg gtaacataag gattaccggc gacctgaagc
     5341 agcgttacgc cggaagccaa aataaacaac gcgcccaaaa ataccgcgta agaatggctg
     5401 cccgcagcag gataaaacag caggcacccg accgccgtca gcagaaagcc gccgataacg
     5461 ccgtttttgt agccgacttt gccgacaaaa gcccccatcg ggatggacat taccgcatag
     5521 gcggtaaaaa agcaaaattg aatcagcatc gcctgaacgt aagagaggtc gaaaatttct
     5581 ttcaaatgag ggataaggat gtcgttcatg caggtaataa aacccatcat gaagaacagc
     5641 gtggtcaaga cgaccaatgg ggacgtatgg ttgttttgtg attgcgcaga catgatttgc
     5701 ctttcgtggt gtcggaatat tgcccgtttc aagaaaatag aaattgacat ttcttaacgg
     5761 caatgcaaat catactgata tttgaacatt ttgcaaactt tatcggatgc aggcaatgcg
     5821 ccgatctgaa attcaagcag cagaaattaa tattttacag ttgtttgcat aggttttaat
     5881 agattttaaa aaattccggc gggcttgcaa aaaccgttcc caataaaatg aaaatcctcc
     5941 gccgtatccg gaaaattcgg aaatgccgca tcggggcatc aaaaccggag cggaaagaat
     6001 gtcgtatgcc cgacaaataa aggggcatac ggctgtgccg tatttttgca ccgtcctccg
     6061 cccttcggcg ggggcgtgaa aataccgtat aatccgtcgc cgatttttac ctgtttggga
     6121 ctgtttgcaa tgataccctc ctacacctac gccgccctcg ctttttccgc cttcacttcc
     6181 gccaccctcc tgcccggcac gtccgaagcc gccttcgccc tgttcgtccg caacttcccc
     6241 aaacacgcat acggcgcgtt gctgtgcgcc ggccttgcca acggattggg cagtatggtt
     6301 tcctattgga tgggacgttt gctgccttcc cgaaaaatgc cgtctgaaaa aacactgaat
     6361 ctgatacggc gtttcggtat ttggctgctt gcgtttgcgt ggctgcccgt cgtcggcgac
     6421 gcactgccgc ttaccgccgg ctggctgcgg ttgaatccgt ggacaagcgg gctgatgctg
     6481 gttatcggca aaacggcgcg ttacgccttc attctgtggg gaatgcaata ttacgccgcc
     6541 tgaaccgtgc gttataatgc cgaccgcaca aaccgactgc acaaaaaaaa tgaacagaca
     6601 aatccgccaa gaaatcttcg agcgtttccg cgccgccaac ccccatccga ccaccgagct
     6661 gaatttcaac tcccctttcg agcttttaat cgccgttctg ctttcggcgc aggcgaccga
     6721 tgtcggcgta aacaaggcga cggcgaagct gtttccggtt gccgatacgc cgcaggcgat
     6781 gctggatttg ggcttggacg gcgtgatgga atacacgaaa accatcgggc tgtataaaac
     6841 caagtccaaa cacattatgc aaacctgccg catcctgctg gaaaaataca acggcgaagt
     6901 gccggaagac cgcgaggctt tggaatcgtt gcctggcgtg gggcgcaaaa cggcaaacgt
     6961 ggtattgaac acagcgttcg gacaccccgt tatggcggtc gatacgcata ttttccgcgt
     7021 gtccaaccga accaaaatcg cacccggaaa agatgtacgc gaagtcgaag acaaactgat
     7081 gcgcttcatt cctaaagaat ttctgatgga cgcgcaccac tggctgattt tgcacggacg
     7141 ctacacctgc aaggctttaa agccgcaatg ccaaacctgc atcatcaacg atttgtgcga
     7201 atatcccgcc aaatcctgac cgccgccgcc cttcaaaatg aaacgcttga aaattcggac
     7261 ggacgttgta gaatttcaac ctatgccgtc tgaagccgac aaaatgcgct tcagacggca
     7321 ttaaagccgg taccggcatc cgacaaagga caaaacgtgt tcaaaaaata ccaatacttc
     7381 gctttggcgg cactgtgtgc cgccttgctg gcaggctgcg aaaaggcagg cagctttttc
     7441 ggtgcggaca aaaaagaagc atccttcgta gaacgcatcg aacacaccaa agacgacggc
     7501 agtgtcagta tgctgctgcc cgactttgcc caactggttc aaagcgaagg cccggcagtc
     7561 gtcaatattc aggcagcccc cgccccgcgc acccaaaacg gcagcggcaa tgccgaaacc
     7621 gattccgacc cgcttgccga cagcgacccg ttctacgaat ttttcaaacg cctcgtcccg
     7681 aacatgcccg aaatccccca agaagaagca gatgacggcg gattgaactt cggttcgggc
     7741 ttcatcatca gcaaaaacgg ctacatcctg accaataccc acgtcgttgc cggtatgggc
     7801 agtatcaaag tcctgctcaa cgacaagcgc gaatataccg ccaaactcat cggttcggat
     7861 gtccaatccg atgtcgccct tctgaaaatc gacgcaacgg aagagctacc cgtcgtcaaa
     7921 atcggcaatc ccaaaaattt gaaaccgggc gaatgggtcg ctgccatcgg cgcgcccttc
     7981 ggctttgaca acagcgtgac cgccggcatc gtgtccgcca aaggcagaag cctgcccaac
     8041 gaaagctaca cacccttcat ccaaaccgac gttgccatca atccgggcaa ttccggcggc
     8101 ccgctgttca acttaaaagg acaggtcgtc ggcatcaatt cgcaaatata cagccgcagc
     8161 ggcggattca tgggcatctc ctttgccatc ccgattgacg ttgccatgaa tgtcgccgaa
     8221 cagctgaaaa acaccggcaa agtccaacgc ggacaactgg gcgtgattat tcaggaagta
     8281 tcctacggtt tggcacagtc gttcggtctg gataaagcca gcggcgcatt gattgccaaa
     8341 atccttcccg gcagccccgc agaacgtgcc ggcctgcagg cgggcgacat cgtcctcagc
     8401 ctcgacggcg gagaaatacg ttcttccggc gaccttcccg tcatggtcgg cgccattacg
     8461 ccgggaaaag aagtcagcct cggcgtatgg cgcaaaggcg aagaaatcac aatcaaagcc
     8521 aagctgggca acgccgccga gcataccggc gcatcatcca aaacagatga agccccctac
     8581 accgaacagc aatccggtac gttctcggtc gaatccgcag gcattaccct tcagacacat
     8641 accgacagca gcggcaaaca cctcgtcgtc gtacgggttt ccgacgcggc agaacgcgca
     8701 ggcttaaggc gcggcgacga aatcctcgcc gtcgggcaag tccccgtcaa tgacgaagcc
     8761 ggtttccgca aagctatgga caaggcaggc aaaaacgtcc ccctgctggt catgcgccgt
     8821 ggcaacacgc tgttcatcgc attaaacctg caataacgca cataatttaa ccaaaatatt
     8881 tttaaatgat aaaatgcccg tcaataccga ttaacgggca ttttacaaaa atcagttggt
     8941 tgattttaaa caaatatttt ttacgacaaa aaatatttcc acccattttg ccaacaaaac
     9001 ccaaaccgga ggcggacaga tacccaccca tccggacgct taacaccccc cataaaccat
     9061 gcagctgatt gactattcac attcattttt ctcggttgtg ccaccctttt tggcactggc
     9121 acttgccgtc attacccgcc gcgtactgct gtctttaggc atcggtattt tggtcggcgt
     9181 tgcctttttg gtcggcggca accccgtcga cggtctgaca cacctgaaag acatggtcgt
     9241 cggcttggct tgggcagacg gcgattggtc gctgggcaaa ccaaaaatct tggttttcct
     9301 gatacttttg ggcattttca cttcactgct gacctactcc ggcagcaatc aggcgtttgc
     9361 cgactgggca aaacggcaca ttaaaaaccg gcgcggcgcg aaaatgctga ccgcctgcct
     9421 cgtgttcgta acctttatcg acgactattt ccacagcctc gccgtcggtg cgattgcccg
     9481 ccccgttacc gacaagttta aagtttcccg cgccaaactc gcctacatcc tcgactccac
     9541 tgcctcgccc atgtgcgtgc tgatgcccgt ttcaagctgg ggcgcgtcga ttatcgccac
     9601 gcttgccgga ttgctcgtta cctacaaaat taccgaatac acgccgatgg ggacgtttgt
     9661 cgccatgagc ctgatgaact attacgcgct gtttgccctg attatggtat tcgtcgtcgc
     9721 atggttctcc ttcgacatcg gctcgatggc gcgtttcgaa caggctgcgt tgaacgaagc
     9781 ccacgacgaa accgccgctt cagacgctac caaaggtcgt gtttacgcat tgattattcc
     9841 cgttttggcc ttaatcgcct caacggtttc cgccatgatc tacaccggcg cgcaggcaag
     9901 cgaaaccttc agcattttgg gggcatttga aaataccgac gtaaacactt cgctggtatt
     9961 cggcggcact tgcggcgtgc ttgccgtcgt cctctgcacg ttcggcacga ttaaaaccgc
    10021 cgattatccc aaagccgtgt ggcagggtgc gaaatccatg ttcggcgcaa tcgccatttt
    10081 aatcctcgcc tggctcatca gtacggttgt cggcgaaatg cacacgggcg actacctctc
    10141 cacgctggtt gcgggcaaca tccatcccgg cttcctgccc gtcatcctct tcctgctcgc
    10201 cagcgtgatg gcgtttgcca caggcacaag ctgggggacg ttcggcatta tgctgccgat
    10261 tgccgccgcc atggcggtca aagtcgaacc cgcgctgatt atcccgtgta tgtccgcagt
    10321 aatggcgggg gcggtatgcg gcgaccactg ttcgcccatc tccgacacga ccatcctgtc
    10381 gtccaccggc gcgcgctgca accacatcga ccacgttacc tcgcaactgc cttatgccct
    10441 gacggttgcc gccgccgccg catcgggcta cctcgcattg ggtctgacaa aatccgcgct
    10501 gttgggcttt agcacgaccg gtattgtatt ggcggtgctg atttttctgt tgaaagataa
    10561 aaaacgcgcc gacgtttgac cttcccccct gttcagacgg catatgccgt ctgaagtttt
    10621 ttggagcaaa ccgcaatgac cgtcccccat attccgcgcg gccccgtcat ggcagacatt
    10681 gccgccttcc gcctgaccga agaagaaaaa caacgcctgc tcgatcccgc aatcggcggc
    10741 atcatcctct tccgccgcaa cttccaaaac atcgaacaac tcaaaacact caccgccgaa
    10801 atcaaagccc tgcgcacacc cgaactcatt atcgccgtcg atcacgaagg cggcagggtg
    10861 caacgtttca tcgaaggttt tacccgcctg cccgcaatga gtacgctcgg cgagatttgg
    10921 gacagtgaag gcgcggaaac tgccgaaact catgccgaac acatcggatg ggttttggca
    10981 accgagcttt ccgcctgcgg catcgatttg tccttcacgc ccgtcttaga tttggactgg
    11041 ggaaactgcg ccgtcatcgg caaccgcagc ttccaccgca atcccgaagc ggtcgcccgc
    11101 ctcgccctcg cccttcaaaa aggtttggca aaaggcggta tgaaatcatg cggcaaacat
    11161 ttccccggac acggatttgt cgaaggggac agccatttgg tgctacccga agacgggcgc
    11221 agcctcgacg aactcgaagc cgccgacctc gcccccttcc gcattatgag ccgcgaaggt
    11281 atggcggcgg tcatgcccgc ccacgtcgtt tatccgcaag tggacacaaa gcccgcaggg
    11341 ttttccgaaa tctggctcaa acaaattttg cgccgcgata tcggattcaa aggcgtgatt
    11401 ttttctgacg atttgaccat ggagggcgcg tgcggggcag gcggcatcaa agaacgcgcc
    11461 cgcatttcct ttgaggcagg ttgcgacatc atattggtct gcaaccgtcc cgacttggtc
    11521 gatgaattgc gcgacggttt caccattccc gacaaccagg atttggccgg acgttggcaa
    11581 tacatggaaa actcgctcgg tcatgaggcg gtacaagccg ttatacagac aacgggtttc
    11641 caagcggcgc aagcctttgt tgccggcctt gcctcgccgc aggacacggc gggcggcgta
    11701 aaagtcggag aggcctttta aaccggctgc gtttcaacaa tctgccgatt tcaaccgttc
    11761 gtcagggaaa ggcaatgatt ttttcggtca aatagaaagt tatcttattg aattactttg
    11821 agataaggat gcttatcaat actgtattca aacacagccc tttaaatccg cccgacacgc
    11881 ggcaaaaagg caaaacggta taggcacgcc gtattttttg cgctataatc acgccttaat
    11941 taattcggaa tgtagcgcag cccggtagcg cacttcgttc gggacgaagg ggtcggaggt
    12001 tcgaatcctc tcattccgac catattcaga ccgcctcgac atcgattgcc ggggcggttt
    12061 ttcccttttg gcggcaacaa aaaaatgccg tctgaaacag aatgctgttt cagacggcat
    12121 ttcgttttcc ccaatcctgc catttgcgcg cactgacaaa gggtatcggc aaggttcaaa
    12181 agaccatcgg aatctaggaa gcctggcgtg tacccaaccg cggcatctta ttcattaagg
    12241 cttcgtcctg attgggcggc ggaaatgtcg gcagtttcac gcggaagcgg cacactttcc
    12301 ccgtatgtca aacatcggca agaaggtacg cctgcccttt gggttacact gcttaaacta
    12361 ctctatcccc gcccatttgt gcgtctgcac gctcaactgc caatgcacgg gcgcgtcggg
    12421 gcggctgttt aaaataccga tttggcggat ggtgtcgtag atgttcatca cgccgttttg
    12481 ttcgcaggga gaaagatagt aatggcgcgc acggattttg cgttccatat tttcgcaaaa
    12541 cgcaaggaca tcgccgtcgg caacaatccg tacttcgtcg gcttcggcga tgcagttggt
    12601 ttcatacttg gcggcgtagc aggctttggg gctggtggcg acatagtcga tttgcggcgg
    12661 cgcgggcttg agcccgttgg tttcgagaca gaggaaatag ccttccgcct tgagcgtgtc
    12721 cagcagcata tcgagatgcg gctggatggt cggctctccg ccggtgatga tgatgttgcg
    12781 ggcggcataa gttttcagac agcctaagat gtcggacagg ctcatcatac caaatgtcaa
    12841 ataatcggta tcacaccagc cgcacgccag attgcatttg cccaagcgga cgaaaacggc
    12901 aggcataccc gtgtttcggc cctcaccttg caggctttca aagatttcga cgatgcggta
    12961 ctggggattt tcgggggcga cgctgatttt tttcataccg ccaacaccgc cgccgccaaa
    13021 aatgcgggca aaccttgttt gacgagtatg cctttgttgc cggacgaaaa cgcaccccac
    13081 gcggcggcaa tcaggacgaa gccgagaaac agtaccgtcg cgccgtacac ggcattatcc
    13141 ggagcggcaa actgcgccca caaaagcccg acggcaagaa agccgttgta cagcccttgg
    13201 ttgccaaaca gggtctgcac gcgtttctgt tccataaatt cgtaaggcag cttgaacatt
    13261 tccgccgctt tttcgccggg aatctgcgtc atttcaagcc aggcgatgta gaaatgttcg
    13321 gcggcgacga ggaggacgag aatggtggag aggagtttca tgggtgttcc tttcaatggg
    13381 tgtgccggta tgaaatgccg tctgaaaaaa cggggaatcc gtgcaggaga aaaaaccgct
    13441 ttacg
//
LOCUS       DAXDXW010000068        13406 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXDXW010000068 DAXDXW010000000
VERSION     DAXDXW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13406
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.069"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            2..1615
                     /locus_tag="ACFB64_001674"
     CDS             2..1615
                     /locus_tag="ACFB64_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC1209995.1"
                     /translation="MRRVRRAWGLRRFCRRRPAVFFKEGKLKDSVYRVCRLCAALCVW
                     GAVGAYAAGLPDVRDDAAALRAQRAAAEGWAGMPPEGDSAANGGSRVIDGDFLLSRPQ
                     LLEHVLRDALNGNQADLIASLADLYAKLPDYDAVLYGRARALLAKLAGRPAEAVARYR
                     ALHRDNAADERVLLDLAAAEFDDFRLKSAGLHFAEAAKLDLPAPVLENVGRFRKKTEG
                     LTGWHFSGGISPSVNKNANNAAPQYCLQGGGSRICSVTGPVRAAGLDYEAGAEKLTAL
                     ADNHYLLFRANVGGTSYYFSRKSAYDDAFGRAYLGWQYKNARQTVGVLPFYQAQLSGS
                     DGFDAKTKPPADRRLAPYMLAHGAGVQLAHSYRLSRRSQLSVSLERYRQRYREQGRAE
                     RNDGWQDGLYVSLARRLGGSATVFGGWQFARFVPKRETVGGAVNNAAYRRNAVHIGWA
                     QEWGGTGGLNSRIAASYAHRNYKGIAAFSTEAQRNREWNVSLALSHDKLSYKGIVPTL
                     NYRFGKTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1676..3550)
                     /locus_tag="ACFB64_001675"
     CDS             complement(1676..3550)
                     /locus_tag="ACFB64_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC1209996.1"
                     /translation="MPYFALFDDAVSRRAKLYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYEFGLPLMGMESERGGNIALHWFADCADTDAESWLARHSDGIPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDEAGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQTD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIRAQALPDTSF
                     ADTLRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLNPCSGGLGFEGAFNV
                     VIRTLSLKPVSDGIVSGIGGTDSNAQARTAGQGGETPHPFEANPPYRGVYGVGSGIVI
                     DSDPAAEYRECGWKARFLNELRPDFGIFETLRAENRQCALLDRHLCRLKTSAQALNLP
                     LPDGCENQIKQYIADLPDGAFRIKALLASDGISLSHAVLNRLTDKQRVIISPTILPAQ
                     NYLRRFKTTHRAIFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQWLTPS
                     LDLDILNGIMRQAVSDEPQKYLHTNQVIETHITQKTLQGAEEIRLSNALRGVFAAVLA
                     "
     gene            3721..4650
                     /gene="ppk2"
                     /locus_tag="ACFB64_001676"
     CDS             3721..4650
                     /gene="ppk2"
                     /locus_tag="ACFB64_001676"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HFC1209997.1"
                     /translation="MTDRQLQPFENVELGEKQDQLQVFEKAVLEHEGKGSAEDSGTAP
                     LPENYPCRKRMRRAVYEAEKAKLQIELLKVQSRVKDSGQRIVSLFEGRDAAGKGGTIK
                     RFMEHLNPRGARVVALEKPTTTERGQWYFQRYIQNLPTAGEMVFFDRSWYNRAGVERV
                     TGFCEPNEYMLFMCQAPELERMLVASGIHLFKFWFSVSREEQLRRFISRRDDALKHWK
                     LSPVDIQSLDRWDDYTEAKNAMFFHTHTGDAPWVIIRSDDKKRARLNCIRYFLHQLDY
                     PGKDVKAIGKVDDKIVLVPDTRYKEKTIDVGHD"
     gene            complement(4732..6163)
                     /gene="aldA"
                     /locus_tag="ACFB64_001677"
                     /pseudo
     CDS             complement(4732..6163)
                     /gene="aldA"
                     /locus_tag="ACFB64_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(6271..7186)
                     /locus_tag="ACFB64_001678"
                     /pseudo
     CDS             complement(6271..7186)
                     /locus_tag="ACFB64_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7359..8159
                     /locus_tag="ACFB64_001679"
     CDS             7359..8159
                     /locus_tag="ACFB64_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC1209998.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            8207..8983
                     /gene="mlaE"
                     /locus_tag="ACFB64_001680"
     CDS             8207..8983
                     /gene="mlaE"
                     /locus_tag="ACFB64_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC1209999.1"
                     /translation="MNFIRSVGAKTLGLIQSFGSITLFLLNILAKSGTAFARPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTGQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWPQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCIPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            9034..9528
                     /gene="mlaD"
                     /locus_tag="ACFB64_001681"
     CDS             9034..9528
                     /gene="mlaD"
                     /locus_tag="ACFB64_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC1210000.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNAEGGNAE
                     KAAE"
     gene            9559..10149
                     /locus_tag="ACFB64_001682"
     CDS             9559..10149
                     /locus_tag="ACFB64_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFC1210001.1"
                     /translation="MKKSSFISALGIGILSIGMAFASPADAVGQIRQNATQVLTILKS
                     GDAASARPKAEAYAVPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKFKNATVNVKDNPIVNKGGKEIVVRAEVGIPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGIDGLIAELKAKNGGK"
     gene            10200..10478
                     /locus_tag="ACFB64_001683"
     CDS             10200..10478
                     /locus_tag="ACFB64_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC1210002.1"
                     /translation="MHTELKNGTLHIGGDITVKTLTGDAFGRFRQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            10475..11302
                     /locus_tag="ACFB64_001684"
     CDS             10475..11302
                     /locus_tag="ACFB64_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC1210003.1"
                     /translation="MKKTAYAILLLIGFASAPAFAETRPADPYEGYNRAVSKFNDQAD
                     RYIFAPAARGYRKVTPKPVRAGVSNFFNNLRDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIVGAGGVPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYPPKNIVFHTPAGRWGTTAAAAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAEPAVHEDSVSETQAEAAGEAETQPGTQP"
     gene            11427..11789
                     /locus_tag="ACFB64_001685"
     CDS             11427..11789
                     /locus_tag="ACFB64_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC1210004.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            11789..12172
                     /locus_tag="ACFB64_001686"
     CDS             11789..12172
                     /locus_tag="ACFB64_001686"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC1210005.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFLEEARWAFFEKRGLMHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGMRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            12169..12657
                     /locus_tag="ACFB64_001687"
     CDS             12169..12657
                     /locus_tag="ACFB64_001687"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC1210006.1"
                     /translation="MKKILTAAAVALIGILLATVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKVIKTANDYKNKDFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVIYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(12891..13019)
                     /locus_tag="ACFB64_001688"
     CDS             complement(12891..13019)
                     /locus_tag="ACFB64_001688"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFC1210007.1"
                     /translation="MAGVTVGRGSVVGAGAVITKDIPPYSLAAGNPAVVKKNLPEG"
     gene            complement(13025..13387)
                     /locus_tag="ACFB64_001689"
     CDS             complement(13025..13387)
                     /locus_tag="ACFB64_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFC1210008.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIGIVGRRVLGFLARRISPHI
                     GRGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMGPECLLYSTNHKFDREKNALRA
                     TRKSVRLRWRTMSGRGAG"
BASE COUNT         2843 a         3764 c         3849 g         2950 t
ORIGIN      
        1 gatgcgccgc gtccgccgcg cgtggggctt gcggcgtttc tgccgccgcc gtccggcggt
       61 gttttttaag gaaggcaaat tgaaggatag tgtgtatcgg gtatgccggc tgtgtgccgc
      121 gctgtgtgtt tggggtgcgg tcggcgcgta tgccgccggc ttgcccgacg ttcgggacga
      181 tgcggcggcg ttgcgcgccc agcgtgcggc tgcggagggt tgggcgggaa tgccgccgga
      241 aggggattct gccgcaaacg gcggttcgcg cgtaatcgac ggggatttcc tgctgtcgcg
      301 cccgcagttg ttggaacacg ttttgcgcga cgcgctcaac ggcaatcagg cggatttgat
      361 cgcttcgttg gcggatttgt atgccaagct gccggattat gatgcggtgt tgtacggcag
      421 ggcgcgggct ttgctggcga aattggcggg aaggccggcg gaggcggtgg cgcgctatcg
      481 ggcgttgcac agggataatg cggcagacga gcgggttttg ctggatttgg cggcggcgga
      541 gtttgacgat ttccggctga agtcggcagg gctgcatttt gcggaggcgg caaaattgga
      601 tttgccggcg ccggttttgg aaaatgtggg gcgtttccgg aaaaaaacgg aggggctgac
      661 gggctggcat ttttcgggcg gcatcagccc ttctgtgaac aaaaacgcca acaatgccgc
      721 gccgcaatac tgcctgcagg gcggcggcag ccggatatgc agcgttaccg gacctgtccg
      781 tgcggcaggg ttggattatg aggccggggc ggaaaagctg actgcgctgg cggacaacca
      841 ttatctgctg ttccgcgcca atgtcggggg cacaagctat tatttcagcc gcaaatcggc
      901 ttatgacgat gctttcggca gggcttattt gggctggcag tataaaaatg cgcggcagac
      961 ggtgggggtt ttgccgtttt atcaggcgca attgtcgggt agcgacggct ttgatgcgaa
     1021 aacgaaaccg cctgccgacc gccggcttgc gccgtatatg ctggcgcacg gggcgggtgt
     1081 gcagttggcg cattcttacc gcctgagccg ccggtcgcag ctttcggtgt cgctggaacg
     1141 ttaccgccaa cgctaccgcg aacaaggccg ggcggaacgc aacgacggtt ggcaggacgg
     1201 gctttatgtg tcgctggcaa ggcggttggg cggctcggca accgtgttcg gcggctggca
     1261 gtttgcgcgg tttgtgccga aacgcgaaac ggtgggcggc gcggtcaaca atgccgccta
     1321 ccgccgcaac gccgtccata tcggttgggc gcaggagtgg ggcgggacgg gcggtttgaa
     1381 cagccggatt gccgcgtctt acgcccaccg caactacaaa ggcattgcgg cattttcgac
     1441 ggaggcgcag cgtaaccgcg aatggaatgt ctcgctggct ttgagccacg acaagttgtc
     1501 gtacaaaggc atcgtgccga cgttgaacta ccgcttcggt aagacggaaa gcaatgtgcc
     1561 gtatgcaaaa cgccgcaaca gcgaggtgtt tgtgtcggcg gattggcggt tttgaatggt
     1621 gggataatgc cgtccgaact ttgcggcagc aggttcggac ggcatttttg cgcgttcagg
     1681 caaggacggc ggcaaatacg ccgcgcaagg cgttggagag gcggatttct tcggctcctt
     1741 gcagtgtttt ttgtgtgatg tgtgtttcaa tcacttgatt tgtgtgcaaa tatttttgcg
     1801 gttcgtccga cacggcttgg cgcattatgc cgtttaaaat gtctaaatct aaagagggcg
     1861 tgagccattg tccgcgatgt ttgacgaaca cgttgctcct gccgccttcg agtaggatgc
     1921 cgtccgaatt gaaaaacagg ctgtcgaacg cgccttgcgt ttcggcggtt tgccacgctt
     1981 ggtcgaagat ggcgcggtgt gtggttttga agcggcgcag gtagttttgt gcgggcagga
     2041 tggtcggcga aatgatgacg cgctgtttgt cggtcagacg gtttaaaacg gcgtgggaca
     2101 ggctgatgcc gtctgaagcg agcagggctt tgatgcggaa cgcgccgtcg ggcaagtcgg
     2161 caatgtattg tttgatttga ttttcgcagc cgtcgggcag gggcaggttg agggcttggg
     2221 cggaggtttt cagacggcat aggtggcggt cgagcagggc gcattgcctg ttttccgcgc
     2281 gcagggtttc aaaaatgccg aaatcggggc gcagttcgtt gaggaatcgg gctttccagc
     2341 cgcattcgcg atattcggcg gcggggtcgc tgtcgatgac gatgccggaa ccgacaccgt
     2401 acacgccccg atacggcgga ttggcttcaa acggatgcgg cgtttccccg ccttgtcctg
     2461 ccgttcgggc ttgcgcgttt gaatccgtgc cgccgatgcc tgaaacaatg ccgtctgaaa
     2521 cgggtttgag cgacagggtg cggatgacga cgttgaacgc gccttcaaac cccaagccgc
     2581 cggaacacgg gttcaaatag ccgatgctgc ccgtataaag cccgcgcgct tcggtttcga
     2641 gcgattcgat aatctgcata ctcatttttt tgggcgcgcc ggtgatgctg ccgcagggga
     2701 aggcggcgcg gagggtgtcg gcgaacgagg tgtccggcag ggcttgcgcc cggatggtgc
     2761 tggtcatctg ccagacgctg ccgaaacgcg atactttaaa cggctcgggt acgcatactt
     2821 taccggtttg ggcgattttg ccgaggtcgt tgcgcagcaa atcgacaatc atcacgtttt
     2881 cggcgcggtt tttcgggtcg gtttgtaact cggcggcgcg gcgttcgtct tgtccgtcgc
     2941 ccaaaatcgg cgcggtgcct ttcatcggtt cggtgctgat ggtgccgtct gaagcgatgt
     3001 tgaggaagag ttcgggcgag aaacacagcg tccacgcgga ttgcccggct tcatcgggca
     3061 ggtgggacaa tacggcatag gggacgggct ggcgcaggcg gcggtagagg ctgacggggt
     3121 tgccgtaggc ttgcaggtgc aggcgggtgg tgtagttgat ttgataggtg tcgccgcgcc
     3181 ggatggcttc gtggatttgg cggatgcggt cgaggtaatc ggtttcggat acggaggatt
     3241 gcggcgtgga aataccggcg gggatgccgt ctgaatgtcg ggcaagccag ctttcggcat
     3301 cggtgtcggc gcagtcggca aaccagtgca gggcaatgtt gccgccgcgt tcggattcca
     3361 tccccatcag cggcaaaccg aattcgtagt ctgcaaacaa caccgaatgc agcccttttt
     3421 gccatcccga ttgcagcgcg ccgtctaaag catcgagttc ttcgggacgg aaaaaacggc
     3481 tttccacatg attttgatag agttttgcgc gtcggctgac ggcatcgtca aacagggcga
     3541 aataaggcat ggcaattcgg ggcaaatgtt ttgattatac gcccctttta cacggatttt
     3601 cagacggcgg ggcaaattcg ggcaacaaaa tgtaatttta tgtagagaaa gcggtggcga
     3661 aagtgtaaaa ttgtgaaaac ggactttcat ttccaacctg aaatacaata ggagaccttt
     3721 atgacagacc gccagttgca accgtttgaa aacgtagaat tgggcgaaaa gcaagaccag
     3781 ctccaagtat ttgaaaaagc tgttttggaa catgaaggca aaggttctgc cgaagattcc
     3841 ggcacagccc cgctgcccga aaactacccc tgccgcaaac gtatgcgccg tgccgtatac
     3901 gaagccgaaa aagccaaact gcaaatcgag ctgctgaaag tgcaaagccg ggtcaaagac
     3961 tccggccagc gcatcgtcag cctgtttgaa ggccgggatg cggcaggtaa gggcggcacc
     4021 atcaaacgct ttatggaaca tttaaacccg cgtggcgcgc gcgtggttgc tttggagaaa
     4081 ccaaccacta ccgaacgcgg acaatggtat ttccaacgct acatccaaaa cctgccgact
     4141 gcgggcgaaa tggtattctt cgaccgctcg tggtacaacc gcgccggcgt ggaacgcgtg
     4201 acgggcttct gcgaacccaa cgaatatatg ctctttatgt gccaagcccc tgagttggaa
     4261 cgtatgcttg tcgccagcgg catccacctg ttcaaattct ggttctccgt atcccgcgaa
     4321 gaacaactgc gccgcttcat ctcccgccgc gacgacgccc tgaaacactg gaaactttcc
     4381 cccgtggaca tccagtcgct cgaccgctgg gacgactaca ccgaagccaa aaacgcgatg
     4441 ttcttccaca cccacaccgg cgacgcgcct tgggtcatca tccgttcgga cgacaaaaaa
     4501 cgcgcccgtt tgaactgtat ccgctacttc ctgcaccagt tggactaccc gggtaaagac
     4561 gtgaaagcca tcggcaaagt ggacgataaa atcgttcttg ttcccgatac gcgttacaaa
     4621 gagaaaacca tcgatgtcgg tcatgactga ttgccggtga agtaataaaa atgccgtctg
     4681 aacccgattt caggttcaga cggcattttc tatccgtgtg tcaaaccggc atcagatgtc
     4741 ggtttccaga taaatgactt gggtttgcag gtattcttcc aaaccgtgtt tgccgtccgc
     4801 gccgccgatg ccggattttt tccagcccgc gtggaagccc tgcatggctt cgaagttttc
     4861 gcggttgatg taggtttcgc cgaattgcag gcggcgggta acgtagaagg cttcgttcag
     4921 gttggtggtg tacacggagc tggtcagacc gaattcgcag tcgtttgcca gggcgatgac
     4981 ctggtcgagc gtgtcgaaag cggaaacggg cagcacgagg ccgaaagttt cttctttcat
     5041 aatgtccata ctgttgtcgg tgtcggtcag cagggtaggc tcgaagaaat agccgcgtcc
     5101 ttcggtgcgt ttgccgccgc aaaccaattt cgcaccttgt ttgacggccc gttccacttt
     5161 ttcggcaacg gctttgacgg cgcgctcttc aatcagcgtg cccatttccg gcgcgcctgc
     5221 ttcggcttcg gcagggttgc cgtagcgcac gcctttcatc gcggcggtca ttttttcaat
     5281 gaacgcgtct ttcaggctgc tgtggacata gacgcgctcg gcgcagttgc agatttgacc
     5341 ggtgttgccg acgcgcgaag ccaagatgga tttcaccgct aaatccaaat ccgcgtcttt
     5401 caacacgatg gcgggggctt tgccgccgag ttccagcgag actttggtga tgttggcgga
     5461 agcggcttcc atcacttggc gacccgcttc aaccgagccg gtcaggctga ccatatcgac
     5521 ttgcggatgg gcggacaagg cgttgccgat ttccgcaccc ggaccgtcca ccacgttgaa
     5581 cacgcctgcg ggcagtccga ccgcatcgat gatttcggcg aagatgtggc agttgatcgg
     5641 ggttacgctg ctgggtttga cgacgatggt gttgcccgta accagatccg ggcccatttt
     5701 gcgggcgatc aggaagaagg ggaagttccg cggcaaaatg ccggcaatca cgcccagcgg
     5761 acgtttgaac gataaaatgt tttcgcgcgg gcggtcgctt tggatgattt cgccttcgta
     5821 acggcgcgcc cattcggctt ggtaatcgag atagtcggca gtgaacatca cttccacacg
     5881 cgccaagtct ttggttttgc cgccttcggc aacgatggtg tcggtcagct cgtcggcgcg
     5941 tccgcgtatg ccttgggcga ttttacgcaa atacgcgccg cgttcgaccg caggcagacg
     6001 ctcccaagcc ggttgcgccg cgcgcgccgc ggtcaacgtc cgccctgccg cctttgggtt
     6061 cgcgggcgat ggtttcttcg gtggacgggt tcaatacgtc gcgccattcg ccgttgaaat
     6121 cgttttcaaa gcgtccgttg atgtacatgg ccaattgttt catttcgggc cctccagttt
     6181 tgtagtcgga tgtagttttg gtttattccc aaataaattg ccgtacaagt ttctttacac
     6241 gcggattttg ggtttcaagt caaatacggc ttaccggttt tcttttttag tttccgcagg
     6301 cgtgctgccg aaatattgcc tgaatgcctg aataaagctg gaaacgtggc gctagccgca
     6361 caaatacgcg gtttcgccca cgtttttgcc cccgttttgc aacagataga gcgcgtgctg
     6421 catttgtttg tggtgcagcc actcgcttgc cgtaatgccg aaatggtcgc gcatacggcg
     6481 ttgcagcgtc ctttcgctga tgttcagcgc ggctgtcagc cggttgactt ggtgtgcgcc
     6541 gccgtcgaac gcggcattca gggtgcggct gaagtcttca gacggcatag cgtctgcttc
     6601 cgccgtttgc cccgccgccg gctcgatgcc gtctgaaacc gtgtcccaca aatccgacag
     6661 cagccgcaac acgtccgcct cgcggcgcaa tgtttcgccc aaatgcccct ttgggacggt
     6721 ttgcaggcag gatgccgcca agccgcgcag gtttgggggc aaatcccata tcctgaccgg
     6781 ttcgcggtaa agcaggggtg cgaaacgcgc gtattccgga cgcggcagcc attgttccat
     6841 acctttaatg gtcattttga ccgttttgcc gccgcgataa agatagcggc tgaacaggac
     6901 ttcttccccg acggcaatta ggacaatctt gccgccgtcc gcatcgatgc ggaagcggcg
     6961 gcggttgatg ccgaagtcca aacgcccttc cagcaacagc acgaacgaca cataaggttc
     7021 ggcgaggcgg ctgctgcaaa aatcgcaccg tgccgttacc gtaccgccgt ggatggaaat
     7081 gccgttggac aaagtatcga aacggtagct gccctccaca taagccgccg ccgcttttga
     7141 ccagattgac gaaccggctg ctgttcaaat gttctgcttt ttccatagtt ttctgataaa
     7201 ggttatcatt tgaaagataa catttttcgc cacagcaaac aatccgccgc cgacaaaggc
     7261 attttcacaa cacttgccgt ccgtatgccg tctgaaaatt gcccgtcttc ccgataagcc
     7321 ttaaaataca cgccgtcaaa cctaccgtcc gcccgtacat gagcccatcc ccctttatcg
     7381 aaatgaaaga cgtcgccttc gcgtatggcg accgcccgat tctgaacgac atcaatttca
     7441 gcattccgca aggcaatttt gccgccgtga tgggcggttc gggcagcggc aaaaccacgc
     7501 tgatgaggct gattacagga cagattcgtc cgcagtccgg gcaggttttg attgaaggac
     7561 gggatttggc gggtttttcg gctgacgaac tctacgaaca ccgccgccgt atgggcgtat
     7621 tgttccaaca cggcgcgctg tttaccgatt tgtcggtatt cgacaatatc gcttttccga
     7681 tgcgcgaact gacgcaactt ccggaagcgg ttattcgcga tttggttttg ttgaaattga
     7741 acgcggtcgg tctgcgcggc gtggaaaacc tgatgccgtc cgagttgtcc ggcgggatgt
     7801 cgcgccgcgt cgcgctggca cgcacaatcg cgctcgaccc cgaaattatg ttgtacgacg
     7861 aaccgtttac cggcctcgac ccgatttcct tgggcgtgat tgcccacttg atcagccgcg
     7921 tcaacaaggc cttgcgttcg accagcatta tggtaacgca cgacattgaa aaatctttgg
     7981 aaatcgtcga tcaggtaatt ttcttggcgc acggcgaaat tatgttctcc ggctcgccgc
     8041 aggaaatgcg cgaactggat tcgccttggg tgcgccagtt tgtcggcggg ctggcagacg
     8101 gccccgtggc ataccgctat ccggcgcaaa cgtcgttgca gcaggatttg ctcgggtaaa
     8161 tgagcaggtg ccgtctgaaa ccgttgaacc ttacatatag aaatttatga attttatccg
     8221 ttccgtcggg gcgaaaaccc tcggccttat tcaatccttc ggcagtatca cgctgtttct
     8281 gctgaacatt ttggcgaaat ccggcacggc tttcgcccgt ccgcgcctga gcgtgcgcca
     8341 agtgtatttt gccggcgtgc tgtcggtgct gattgttgcc gtttcggggc tgttcgtcgg
     8401 tatggttttg ggtttgcagg gctatacgca gttgtcgaaa ttcaaatccg ccgatatttt
     8461 gggctatatg gtcgcggctt ctctgttgcg cgaactgggt cccgtgttgg cggcgattct
     8521 gtttgccagc agcgcgggcg gtgcgatgac cagcgaaatc ggtttgatga aaacgaccgg
     8581 acagctcgaa gcgatgaacg tgatggcggt caaccccgtc gcccgcgtgg ttgccccgcg
     8641 tttttgggcg ggcgtgtttt ctatgccgct tttggcttcg attttcaacg tcgcgggcat
     8701 tttcggcgcg tatttggtcg gcgtgagctg gctgggtttg gacagcggta ttttctggcc
     8761 gcagatgcag aacaacatta cgatacatta cgatgtaatc aacggtttga tcaaatccgc
     8821 cgcgttcggc gtggcggtaa cgctgattgc cgtgcatcag ggcttccact gcatcccgac
     8881 ttcggaaggc attttgcgcg ccagcacgcg cacggtggtt tcgtccgccc tgacgatttt
     8941 ggcggtcgat tttatattga ccgcgtggat gtttacagat tgacagactg tctgaagacg
     9001 aaacgcggaa catcggatat tcaaggaact ttaatgaaaa agaacatatt ggaattttgg
     9061 gtcggactgt tcgtcctgat cggcgcggcg gcggttgcct ttctcgcttt ccgcgtggcg
     9121 ggcggcgcgg cgttcggcgg ttcggacaaa acttacgccg tttatgccga tttcggcgac
     9181 atcggcggtt tgaaggtcaa tgcccccgtc aaatccgcag gcgtattggt cgggcgcgtc
     9241 ggcgctatcg ggcttgaccc gaaatcctat caggcgaggg tgcgccttga tttggacggc
     9301 aagtatcagt tcagcagcga cgtttccgcg caaatcctga cttcgggact tttgggcgaa
     9361 cagtacatcg ggctgcagca gggcggcgat acggaaaacc ttgctgccgg cgacaccatc
     9421 tccgtaacca gttctgcaat ggttctggaa aacctgatcg gtaaattcat gaccagcttc
     9481 gccgagaaaa acgctgaggg cggcaatgcg gaaaaagccg cagaataaac atacacatcc
     9541 attttcagga aagacatcat gaaaaaatcc tccttcatca gcgcattggg catcggtatt
     9601 ttgagcatcg gcatggcatt tgcctccccg gccgacgcag tgggacaaat ccgccaaaac
     9661 gccacacagg ttttgaccat cctcaaaagc ggcgacgcgg cttctgcacg cccaaaagcc
     9721 gaagcctatg cggttcccta tttcgatttc caacgtatga ccgcattggc ggtcggcaac
     9781 ccttggcgta ccgcgtccga cgcgcaaaaa caagcgttgg ccaaagaatt tcaaaccctg
     9841 ctgatccgca cctattccgg cacgatgctg aaattcaaaa acgcgaccgt caacgtcaaa
     9901 gacaatccca tcgtcaataa gggcggcaag gaaatcgtcg tccgtgccga agtcggcatc
     9961 cccggtcaga agcccgtcaa tatggacttt accacctacc aaagcggcgg caaataccgt
    10021 acctacaacg tcgccatcga aggcgcgagc ctggttaccg tgtaccgcaa ccaattcggc
    10081 gaaatcatca aagccaaagg catcgacggg ctgattgccg agttgaaagc caaaaacggc
    10141 ggcaaataac cgacaaacca taccgtccga gggcggtatg ggtttttcga acaaacgcta
    10201 tgcatacaga actcaaaaac ggaacactgc acatcggcgg cgacatcacc gtcaaaaccc
    10261 tgaccgggga cgcatttggg cgtttccggc aacaatgccg tctgaaagaa accattgccg
    10321 tcgatttcgg aggtgtcaaa cgtgccgatt ccgcctgcgt gtcgctgctg ctcgaagtgc
    10381 tgcgcggctg caaaggcagc gtcaggctga ccggcattcc cgaatccgtg cgcgcgctgt
    10441 ccgaactgta cgaaatcaaa gactggctga aatcatgaaa aaaaccgcct acgccatcct
    10501 cctgctgatc gggttcgctt ccgcccctgc atttgcagaa acccgccccg ccgaccctta
    10561 tgaaggctac aaccgcgccg tttccaaatt caacgaccaa gccgaccgct acattttcgc
    10621 ccctgccgcg cgcggctacc gcaaagttac gccgaaaccc gtccgcgccg gcgtgtccaa
    10681 tttttttaac aacctgcgcg acgtggtcag tttcggcagc aatatcttgc gtttggacat
    10741 caaacgcgca agcgaagacc tcgtccgcgt cggcatcaat accaccttcg gtttgggcgg
    10801 gctcattgat attgtcggcg cgggcggcgt tcccgacaat aaaaacactt tgggcgacac
    10861 gtttgcctcg tggggctgga aaaacagcaa ttatttcgtg ttgcccgtct taggcccgtc
    10921 caccgtccgc gacgcgctcg gcacgggcat tacctctgtt tatccgccca agaatatcgt
    10981 tttccatacc cctgccggac gctggggcac gactgccgct gccgccgtca gtacgcgcga
    11041 aggcctgctc gatttgaccg acagtctgga cgaagccgcc atcgacaaat acagctacac
    11101 gcgcgacctc tatatgaaag tccgcgcacg gcagaccggt gcaacacctg ccgaaggtac
    11161 ggaagataac atcgacatcg acgaattggt cgaaagtgcc gaaaccggcg cggcagagcc
    11221 cgccgttcac gaagattccg tatccgaaac acaggcagaa gcagcagggg aagccgaaac
    11281 gcaacctgga acacaaccct aaagccatgc cgtctgaaaa tccgtgttcg gacggcattc
    11341 ccttccgtcg gaacggacag tccgacacta cagattgggc tataatccct tttttgcgca
    11401 taccgcgcga cagcaaagga aaaaccatgt acgaagtcaa ccgcagcgta ttcgtcctca
    11461 tccctttaga acctttttgg aactggctgc aaaccctgcc gggcaaccac ctcgacggac
    11521 tgaccctcga agacatccaa gccgatgcca attcctacct tgtccgcccg tgcgaaaccg
    11581 ccgacgaagt atgggacgaa atcgaagccc gtttcgaaga cattttcgcc gccgaacttg
    11641 ccgactggtg cgaagacgaa cgcgagtggc ccgcactcga tgccgacatt ttcaacgaat
    11701 ggttcgacat ccagctttct accgtcatca ccgacctcga acacgaaccg cttgcccgcg
    11761 aagccttcca acccatcaac ctgaactgat gaagctgacc gtccgcaatt accatctcga
    11821 cggctacggt catgtcaaca atgcacgcta cctcgaattt ctcgaggaag cgcgctgggc
    11881 gttttttgaa aaacgcggac tgatgcacga gttggcaggc ctcatactga ttgtcgcccg
    11941 catcgacatc cgatacagcc gtcccgccgt cgaaggcgac gtattgcagt tttcatgccg
    12001 cctgaaaacc cccggcatgc gccgcattgt gctgacccag acgattacac tgccaaacgg
    12061 caaaaccgcc gcagaagccg acatcaccct gatgcccgtc cacgccgcca cacaacgcac
    12121 cgtcagcctg cccgccaccc ttgcccgcgc actggaagcc ttgtccgaat gaaaaaaata
    12181 ctcaccgccg ccgccgtcgc actgatcggc atcctccttg ccaccgtcct catccccgac
    12241 agtaaaaccg cgcccgcctt ctccctgccc gacctgcacg gaaaaaccgt ttccaacgcc
    12301 gacctgcaag gcaaagtcac cctgattaat ttttggtttc cctcctgtcc gggttgtgtg
    12361 agcgaaatgc ccaaagtcat caaaacggca aacgactaca aaaataaaga tttccaagtc
    12421 ctcgccgttg cccagcccat cgatccgata gaaagcgtcc gccaatacgt caaagactac
    12481 ggactgccgt ttaccgtcat ttatgatgcg gacaaagccg tcggacaggc attcggcaca
    12541 caggtttatc cgacttccgt ccttatcggc aaaaaaggcg aaattctcaa aacctacgtc
    12601 ggagaacccg atttcggcaa actctaccaa gaaatcgata ccgcgctggc acaatagcga
    12661 tgccgtctga agcgcaaaaa aagcaggaac gatgttcctg ttttttataa tggattaaca
    12721 aaaaccggta cggcgttgcc ccgccccggc tcaaagggaa cgattcccta aggcgcccaa
    12781 gcaccaagcg aaccggttcc gtaccatttg tactgcctgc ggcccgccgc cttgtcctga
    12841 tttttgttaa tccactatag attatccgcc ccgacacgtt caggcggcat tcaaccttcc
    12901 ggcagattct ttttcaccac cgcaggattg cctgccgcca aagagtaggg cggaatgtct
    12961 tttgtaatca ccgcgcctgc gcccacgacc gagccgcgtc cgacggttac gcccgccatc
    13021 acaatcaccc tgcgccccgg ccagacatcg tcctccaacg taatcggacg gatttccgtg
    13081 tagccctcaa agcgtttttt tcacggtcaa acttgtggtt ggtcgaatag agcaggcatt
    13141 ccggccccat catcacattt ttgccgacca ccagcccacg gcagatttca cagtttgccc
    13201 cgatgcccga gccgtcgccc aaaaccgtat ccggaaacac atacgccccg cgtccgatat
    13261 gcggcgaaat ccgccgcgcc agaaaaccga gcacccgtct gccgacgatg ccgatgccgc
    13321 gcatatgcga cggcggcaac accgaaccgg caaaacgcag cagcaggaaa aagaattttg
    13381 atcccatcaa acctccctca aaagaa
//
LOCUS       DAXDXW010000069        12694 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXDXW010000069 DAXDXW010000000
VERSION     DAXDXW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12694
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.070"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..365
                     /locus_tag="ACFB64_001690"
     CDS             <1..365
                     /locus_tag="ACFB64_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC1210009.1"
                     /translation="GIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMG
                     APVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSG
                     KYAFARRIKKNDPSRFLN"
     gene            384..602
                     /locus_tag="ACFB64_001691"
     CDS             384..602
                     /locus_tag="ACFB64_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210010.1"
                     /translation="MSMPEMPKWYGDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRAYLLALIEGLENPYRKV"
     gene            747..1091
                     /locus_tag="ACFB64_001692"
     CDS             747..1091
                     /locus_tag="ACFB64_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC1210011.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            1091..1783
                     /locus_tag="ACFB64_001693"
     CDS             1091..1783
                     /locus_tag="ACFB64_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC1210012.1"
                     /translation="MSEILRQPGILLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFRLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLPLASKSVTNPIAIEITSSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            1850..3070
                     /gene="argJ"
                     /locus_tag="ACFB64_001694"
     CDS             1850..3070
                     /gene="argJ"
                     /locus_tag="ACFB64_001694"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC1210013.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDQHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTCDEARQAAYAAARSPLVKTAFFASDPNLGRLLAAIGYADVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKDEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            3130..4538
                     /locus_tag="ACFB64_001695"
                     /pseudo
     CDS             3130..4538
                     /locus_tag="ACFB64_001695"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            4726..6117
                     /gene="hrpA"
                     /locus_tag="ACFB64_001696"
     CDS             4726..6117
                     /gene="hrpA"
                     /locus_tag="ACFB64_001696"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC1210014.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKIEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLEEIKKTVAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKCRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLNLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEEN"
     gene            complement(6479..7165)
                     /locus_tag="ACFB64_001697"
     CDS             complement(6479..7165)
                     /locus_tag="ACFB64_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210015.1"
                     /translation="MENKQTEKTETQSEKTKKKPFKQTRELVRLALNNGWTQTEIAKT
                     CRTQQSVVSAWSKGEKLGTEQQLKPLLDLFGYKLRRNTFKLYWRKNAETSKIEFIKVE
                     GKIIFSLLIGKNNGYNKFVPAYKFIIHYQGNEQFYMILKKKIYDDYDKSLEAAAWQTI
                     LNQSIGISELLRFFDKLSSKDIENMGRFWLEFCCNFPKYTQSLPFLIRQALLNHGFSV
                     NDIIEYQASW"
     gene            complement(7236..7388)
                     /locus_tag="ACFB64_001698"
     CDS             complement(7236..7388)
                     /locus_tag="ACFB64_001698"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210016.1"
                     /translation="MSNKQANHQSNMSNANKGTSGTNKTYSQNQGNRGWQMNPQNPAN
                     SGKGKK"
     gene            7849..9414
                     /gene="lpt3"
                     /locus_tag="ACFB64_001699"
     CDS             7849..9414
                     /gene="lpt3"
                     /locus_tag="ACFB64_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC1210017.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FMIAALYLFARYKASRLLIAVFFAFSMIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVAEVVLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSKIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKQSYSAGFMTAVSLPSFFNVIPHANG
                     LEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLGYGNG
                     DNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIADKYDNT
                     IHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYIVPLVLYS
                     PDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGDAGSL
                     NIRNGKAEYVYPQ"
     gene            9516..>12694
                     /locus_tag="ACFB64_001700"
     CDS             9516..>12694
                     /locus_tag="ACFB64_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC1210018.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHERFTDKQSDFLTYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRPPEVKQLTSSENAGDQDLSAKLKQKQLDKKQHRTQIRATKEAGYEQIHRALLTGL
                     IANVGMKSPDSNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTRLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLAVLPRRPVSYGKVAPEEAR
                     EIFIRSALVAQEYDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYHERLP
                     DFYMADSVSEGLCPANPQQTTPSPVGEGWGEGKTVAAQTNFSATSASPLPNPLPQERE
                     QSASVSTVSGSLKTMSCEARLNFCEAKTKTESSLHSQRLSENYTPPFSDGLRPANPQQ
                     TTPSPVGEGWGEGKTVAAQTNFSATAANPLPQEREQSASALTVSDDPKPKKQPASQKG
                     RLKPLPLADIRTFEAWLKTAERDNPRLLFLSRDDLMQHAAAHITEEQFPKHWQTADGK
                     FKLSYRFEPHHPLDGVTLTLPLTVLNRISPAALEWLVPGMIREKIQLQIKALPKQIRR
                     ICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRILEQINQDEWAAFRLPEHC
                     YFNLRIIDDGGQELAMGRDLIQIQQQLGKAATTTFRDNTQEFERDNVTAWDIGILPES
                     IKFARGKQQLTGYLGLQKEKDGRIALRLFDTSAAAGHAHRLGVIELMKLQLKEQVKDL
                     NKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDKLPRNEKAFKEQIKRARSR
                     LPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRLQTLLAPGFATRTPWAQWPR
                     LPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDGLAKQGQPVSDGLAAFK
                     WMIEELRVSLFAQELKTPYPVSVKRLLKM"
BASE COUNT         3085 a         3763 c         3047 g         2799 t
ORIGIN      
        1 tgggtattgc ctaccgctac ggcggcacat cggtttctac cggttttgac tgcagcggat
       61 tcatgcagca catcttcaaa cgcgccatgg gcatcaacct gccgcgcacg tcggcggaac
      121 aggcgcggat gggcgcaccc gttgcccgaa gcgaattgca gcccggggat atggtgtttt
      181 tccgcacgct cggcggcagc cgcatttccc atgtcggact ttatatcggc aacaaccgct
      241 tcatccacgc gccgcgcacg gggaaaaata tcgaaatcac cagcctgagc cacaaatatt
      301 ggagcggcaa atatgcgttc gcccgccgga tcaagaaaaa cgacccgtca cgctttctga
      361 actgatttcc caaggaatac gcaatgagta tgcccgaaat gcccaaatgg tacggcgatg
      421 acggacagat cgtatcctgt accgaaaagg tcaaagtgat gtccgaaaat atggccgagc
      481 tgtatcagac ggcacaagac gcgtttgaag acgcgctgct gatgggttgc ggcgaacggc
      541 agttgcgcgc ttacctgctc gcgctgattg aaggtttgga aaatccctac cgcaaagtct
      601 gaacacgccc cggttgctgc ggcacggttt atccgtgccg tttttgcgtt tgtgcgcggc
      661 ttcggctttt cagacggcat atttgacgtt atgattaaac agttaacaag atttatcaca
      721 acgccgtcaa aagacagaca cacaacatga acatcatccg cgcgctcctc atcatcctcg
      781 gctgcctcgc cgccggcgaa accgccgttt tcctagcagg catcaaactg cccggcagca
      841 tcgtcggcat gggcgtgctg tttgcgcttt tgcaggcggg ttgggtcaaa acgtcttggc
      901 tgcaacagct taccgacgcg ctgatggcaa acctgacgct gttcctcgtg ccgccctgcg
      961 tggcggtcat cagctatttg gatttgattg ccgacgattg gttttcgata ctggtttccg
     1021 cctccgccag cactttgtgc gtactgctgg ttacgggcaa ggttcaccgc tggatacgga
     1081 gcattatctg atgagcgaaa tcctcaggca gcccggcatc ctgcttttcc tcacgcttgc
     1141 cgtgtacgcg cttgcgatta tcgtgcgcac gcgcacgggc aatatcttct gcaaccccgt
     1201 actcgtcagc actatcgtgc tgattgccta cctgaaaatc ctcggtatcg attatgcggt
     1261 gtaccacaac gccgcgcaat tcattgattt tcggctgaaa cccgccgtcg tcgtgcttgc
     1321 cgtgccgctc taccaaaacc gccgtaaaat cttcaaccag tggctgcccg tcatcgtttc
     1381 gcagcttgcg ggcagcgtta cgggcattgt tacggggatg tattttgcca agtggctggg
     1441 cgcggaacgc gaagtcgtcc tcccgctcgc gtccaaatct gttaccaacc ccatcgccat
     1501 cgaaatcacc agctccatcg gcggcattcc cgccattacc gccgccaccg tcatcattgc
     1561 cggtctggtc ggacagattg ccggttacaa aatgttgaag aacacggtcg tcatgccctc
     1621 gtccgtgggt atgtcgctcg gcacggcttc gcacgcgatg ggcattgccg cctcgctcga
     1681 acgcagccgc cgcatggcgg catacgcggg gctggggctg acgttcaacg gcgtgctgac
     1741 cgcgctgatt gcgccgctgc ttatccccgt tttggggttc tgaacccgtt tcagacggca
     1801 tttcaaccca tgccgtctga acgccgacac actcgcaagg agaaccgtta tggctgtcaa
     1861 cctgaccgaa aaaaccgccg aacaactgcc cgacatcgac ggcattgccc tctacaccgc
     1921 ccaagcaggc gtgaagaagc ccgggcatac cgacctgaca ctgattgccg tagccgccgg
     1981 cagcaccgtc ggtgcagtct tcacgaccaa ccgtttctgt gccgcgcccg tccacatcgc
     2041 caaatcgcac cttttcgacg aagacggcgt gcgcgccctc gtcatcaaca cgggcaacgc
     2101 caacgcgggt acgggcgcac agggcagaat cgatgctttg gcagtgtgtg ccgccgccgc
     2161 ccggcaaatc ggctgcaaac cgaaccaggt gctgcccttc tccaccggcg tgattctcga
     2221 accgctgccc gcagacaaaa tcatcgccgc cctgcccaaa atgcagcctg ccttctggaa
     2281 cgaagcggca cgcgccatca tgaccaccga caccgttccc aaagccgcct cgcgcgaagg
     2341 caaggtcggc gaccaacaca ccgtccgcgc cacaggcatt gccaaaggct cgggcatgat
     2401 tcatcccaat atggcgacca tgctcggttt catcgccacc gatgccaaag tttcccaacc
     2461 cgtcctccaa ctgatgacgc aggaaatcgc cgacgaaacc ttcaacacca tcaccgttga
     2521 cggcgacacc agcaccaacg acagcttcgt catcatcgcc accgggaaaa acagccaaag
     2581 cgaaatcgac aacatcgccg acccgcgtta cgcccaactc aaagaattgt tgtgcagcct
     2641 tgcgctcgaa ctcgcccaag ccatcgtccg cgacggcgag ggcgcgacca agttcatcac
     2701 cgtccgcgtc gaaaacgcca aaacctgcga cgaagcccgc caagccgcct acgccgcggc
     2761 acgttcgcca ctggtcaaaa ccgccttttt cgcctccgac cccaacctcg gcaggctgct
     2821 cgccgccatc ggttatgccg acgttgccga cctcgatacc gacctcgtgg aaatgtatct
     2881 cgacgatatt ttggttgccg aacacggcgg acgcgccgca agctacaccg aagcacaagg
     2941 gcaggcggtg atgtcgaagg acgaaatcac cgtccgcatc aagctgcatc gcggacaagc
     3001 cgccgccacc gtctatacct gcgacctgtc gcacggatac gtttccatca acgccgacta
     3061 ccgttcctga cccgacacgg cttcagacgg catacataaa atgccgtctg aaccgtcgga
     3121 caacatacca tgacctccac attcccccgc cgcctcgccc gcaaaatccg ccaaacccgc
     3181 cgcctgtcgc gcaaaagcat cgcctttctg ttccttctgg caggttcggc actcgtcgcc
     3241 ctgaccgcac tgttttttgc ccatcttgcc gattttgcgc tggaactgaa cgccaaactg
     3301 gttcaacaat acccgtggtt cgcatggatt gccctgccgc cgggactgcc cgtcatcgtc
     3361 tggctcacac gcaaattcgc ccccttcacc gccggcagcg gcatccctca agtcatcgcc
     3421 tcactgtcgc tgccctacgg cgcacaaaaa acgcggctga tacgcctcgg gcaaacgctg
     3481 ctgaagattc cgctgacctt tttgggtatg ctgttcggcg cgtccatcgg acgcgaaggc
     3541 ccgtccgtgc aggtcggcgc ggcagtaatg ggcgcgtggg gcgcgtggtg caaaaaacac
     3601 ggtttggcgt ttaagggtat gcaggaaaac gatttgatgg cggcgggcgc ggcgggcggt
     3661 ttggcagcag cgttcaacgc gccgctggcg ggcgtgattt tcgccattga ggaactgggg
     3721 cgcggcatca tgctgcgctg ggagcggcag attctgctcg gcgtactcgc cgccggtttc
     3781 atacaggtcg ccattcaggg caacaacccg tatttttcgg gtttcaacgg cggcgttttg
     3841 gaaaatattt tgatgtggat cgtggcggcg gggctggttt gcggcgcggc tggcggcata
     3901 tttgcccgta tgctccatcg cggtgcggcg gcgtttgcac cgcgcaagat acgcggcttc
     3961 atccgcaacc gtccgctgct gctggcggca ctgatggggc tgctgctcgc cctgctcggc
     4021 acgttctacc aaggcaaaac ctacggcacc ggctaccacg aagccgccca agccctgcac
     4081 ggcatctacg aagccccgtc ggactcgccg ccgccaaatg gctcgccacc gtattcagct
     4141 attgggcagg catcccgggc ggcattttca ctccctcgct gaccataggt gcggttttgg
     4201 gcgagcatat cgccgccatc gccgacatat cgcagggtgc aaacatcatc gtcctcatct
     4261 gcatggcggc atttttagcg ggcgcgacac aatcgccgat tacttccgcc gtcgtcgtca
     4321 tggaaatgac cggcgggcaa agcctgctgt tttggatgct gattgcctgc attttcgcct
     4381 cgcaggtttc gcgccagttt tcgccgcgtc cgttctacca cgcatcggga atgcgcttcc
     4441 gccggcgcgt gcttcaggaa accgccgccc aaaccggcaa tgcgcccgca agaccgcaag
     4501 ccgcaaacag caaaacggga atgccgtctg aaaattaaaa cgcccccgat caaacgccgg
     4561 cagccgcctt gattcaaata ccgttccgct gcgcttgaaa tttcagcaac aatgccgtct
     4621 gaacgacaga atgcggtttt cagacggcat ttccccatcc cgatattgtt caaacaaaac
     4681 cgaagcgttt gctataattg cactttttac cgcacacgca cgcccatgtt tcccgatttc
     4741 tcccaaaccc tctccaaaga ccgccacttc ctgcaatccg ccttcaaaaa tcccaacaaa
     4801 tacggcggct tgtccaaaat cgaagaaaaa taccgaaaat cgcacgaaat cttcttgaaa
     4861 cgtttggccg cattgccaaa acccgaattc gacaacaccc tgcccgttca cgaaaaactc
     4921 gaagaaatca aaaaaaccgt tgccgaaaat caggtaacga ttatctgcgg cgaaaccggt
     4981 tcgggcaaaa ccacgcagtt gcccaagatt tgtttggaac tcgggcgtgg ggcggcagga
     5041 ttgatcgggc atacccagcc gcgccgtttg gccgcgcgtt cggtggcgga gcggattgcc
     5101 gaagagctga aatccgaaat cggcagcgcg gtcggctata aagtgcgctt taccgaccac
     5161 acctcgcgcg atgcctgcgt caagctgatg accgacggca tcctgctggc ggaaacccag
     5221 accgaccgtt atctcgccgc ctacgacacg attatcattg acgaagcgca tgaacgcagc
     5281 ctgaacatcg acttcctctt gggctaccta aaacaactcc tgccgcgccg ccccgatttg
     5341 aaagtcatca tcacatcagc caccatagac gcagaacgct tctcccgaca cttcaacggc
     5401 gcgcccgtac tggaagtgag cgggcgcacc tatcccgtcg aaatcctcta ccgaccgctg
     5461 accggcaaag acgaagacga cgcagaagtc gagctgaccg acgcgattgt cgatgccgcc
     5521 gacgaattgg cacgctacgg cgaaggcgat attttagtat tcctgccggg cgaacgcgaa
     5581 atccgcgaag cggcagaagc cctgcgcaaa tccacactgc gccgcaatga cgaaatcctg
     5641 cccctgtttg cacgcctgtc gcacgccgaa cagcacaaaa tcttccaccc ttcaggcgca
     5701 aaatgccgca tcgtgctggc gaccaacgtc gccgaaacct cgctcaccgt gccgggcatc
     5761 aaatacgtca tcgacaccgg cctcgcgcgc gtcaaacgct attccgcgcg ggcaaaagtg
     5821 gaacaacttc acgtagaaaa aatctcccaa gccgccgccc gccaacgctc cggccgctgc
     5881 ggacgcgtct cagcaggcgt gtgtatccga ctgttttcag aagaagattt taacagccgt
     5941 cccgaattta ccgatcccga aatcgtccgc agcaacctcg ccgccgtcat cctgcgcatg
     6001 gcatcgctga acttgggcga cgtagcagca ttcccatttt tagaaatgcc cgattcgcga
     6061 tatatcaatg acggttttca ggtgttgctg gaattggggg cagtggagga gaattagatt
     6121 gaaagcaggc tacctgaaaa tgtgaagcag cctgcacttt taaggttaaa ttgaataaag
     6181 ttcagatgac ttaaacgtag aaaaccaagc ggtcagctaa ctgacggctt ggtttttgac
     6241 ccacccctga ccctaattct tacaacggtg tttcaggtat tcagtttccc tttctcctag
     6301 ctgtattcac agcaacagtt gtaagccgag attttgattt acctttgcgc tatgaataca
     6361 aacaggcatt tcatagggct taatcaataa tcatgatgat aaatcgtcag gcttaatatt
     6421 gcccaatgaa tagcatttta tttaaggctg ctttcctgcc ttgggggttt aaggtatttt
     6481 accatgaagc ctgatattca ataatatcgt ttacagagaa tccatgattt aacaaggctt
     6541 gccgaataag aaaaggtaaa gattgagtat atttagggaa gttacaacaa aattccaacc
     6601 aaaacctgcc catattttca atatctttac tacttaattt atcaaaaaat cgaagtaact
     6661 cgctaattcc tattgattga tttaaaatag tttgccatgc tgctgcttcc aaacttttat
     6721 cataatcgtc ataaattttc ttttttaaaa tcatataaaa ttgttcattc ccttgataat
     6781 gaatgataaa tttatatgct ggtacaaatt tattatagcc attgttttta ccaataagaa
     6841 gactaaaaat tatttttcct tctactttaa taaattcaat tttagatgtc tctgcatttt
     6901 tgcgccaata cagcttgaat gtgttcctac gtaatttgta tccgaataaa tccaataaag
     6961 gtttgagctg ttgctctgtt cccaattttt cgcccttgct ccatgcactc acaactgatt
     7021 gttgtgtgcg gcaagtttta gcaatttcag tttgcgtcca accattattc aatgccagcc
     7081 taacgagttc tctcgtctgc ttaaatggtt ttttctttgt cttttcagat tgtgtttctg
     7141 tcttttcggt ttgtttgttt tccataatac ccccaatatg ccaattaaaa tggcgatacc
     7201 aagtataata tcgccattta taggcaaggc aatggctatt ttttgccttt accgctattc
     7261 gcaggatttt gtggattcat ttgccagcca cgatttccct gattttgtga atacgtttta
     7321 tttgttcctg acgtgccttt attagcattg ctcatgtttg attgatgatt ggcttgttta
     7381 ttgctcattt tagttctact cctgaaaaag atatttaatc tgcttggtga aactgtggat
     7441 tgctttgcaa tcgctcggtg ttgatgggat tgcaattcta cggttatttg gtgctaccaa
     7501 aatattgtgc ttttttctat tttgtatttt ccacccccta tttagtagtg aatgaataaa
     7561 agaattgatt tttgaattgg tgctaaattt tttcatgatt accccccatg acgataggta
     7621 gcatcataaa aaattcgtag caaaagttcc attgataaaa agttgataaa ttttgatatt
     7681 ttatgatatt ttgtaattta ttgatattta atgattttaa gatagacatt ctatggattt
     7741 aagatttact tctcgttttt tgaaaaacaa tctgtaaaca gatagctgct gaagaatacc
     7801 gttgccgagc cccaaaaccc gtcctgcaac ttttattgtg aactgcccat tatgaaaaaa
     7861 tcccttttcg ttctctttct gtattcatcc ctacttaccg ccagcgaaat cgcctatcgc
     7921 tttgtattcg gaattgaaac cttaccggct gcaaaaatgg cggaaacgtt tgcgctgaca
     7981 tttatgattg ctgcgctgta tctgtttgcg cgttataagg cttcgcggct gctgattgcg
     8041 gtgtttttcg cgttcagcat gattgccaac aatgtgcatt acgcggttta tcaaagctgg
     8101 atgacgggta ttaactattg gctgatgctg aaagaggtta ccgaagtcgg cagcgcgggc
     8161 gcgtcgatgt tggataaatt gtggctgcct gctttgtggg gcgtggcgga agtcgtgctg
     8221 ttttgcagcc ttgccaagtt ccgccgtaag acgcattttt ctgccgatat cctgtttgcc
     8281 ttcctaatgc tgatgatttt cgtgcgttcg ttcgacacga aacaagagca cggtatttcg
     8341 cccaaaccga catacagccg catcaaagcc aattatttca gcttcggtta ttttgtcgga
     8401 cgcgtgttgc cgtatcagtt gtttgattta agcaagatcc ctgtgttcaa acagcctgct
     8461 ccaagcaaaa tcgggcaagg cagtattcaa aatatcgtcc tgattatggg cgaaagcgaa
     8521 agcgcggcgc atttgaaatt gtttggttac gggcgcgaaa cttcgccgtt tttaacccgg
     8581 ctgtcgcaag ccgattttaa acaaagttat tccgcaggct ttatgacggc agtatccctg
     8641 cccagtttct ttaacgtcat accgcacgcc aacggcttgg aacaaatcag cggcggcgat
     8701 accaatatgt tccgcctcgc caaagagcag ggctatgaaa cgtattttta cagtgcccag
     8761 gctgaaaacc aaatggcaat tttgaactta atcggtaaga aatggataga ccatctgatt
     8821 cagccgacgc aacttggcta cggcaacggc gacaatatgc ccgatgagaa gctgctgccg
     8881 ttgttcgaca aaatcaattt gcagcagggc aggcatttta tcgtgttgca ccaacgcggt
     8941 tcgcacgccc catacggcgc attgttgcag cctcaagata aagtattcgg cgaagccgat
     9001 attgcggata agtacgacaa caccatccac aaaaccgacc aaatgattca aaccgtattc
     9061 gagcagctgc aaaagcagcc tgacggcaac tggctgtttg cctatacctc cgatcatggc
     9121 cagtatgtgc gccaagatat ctacaatcaa ggcacggtgc agcccgacag ctatattgtg
     9181 cctctggttt tgtacagccc ggataaggcc gtgcaacagg ctgccaacca ggcttttgcg
     9241 ccttgcgaga ttgccttcca tcagcagctt tcaacgttcc tgattcacac gttgggctac
     9301 gatatgccgg tttcaggttg tcgcgaaggc tcggtaacag gcaacctgat tacgggcgat
     9361 gcaggcagct tgaacattcg caacggcaag gcggaatatg tttatccgca ataagcagcg
     9421 tgaaaaccaa taaagacaaa tttagatgat gtcggggaag atgcccgacc gacaagacta
     9481 tgcaaaatat gaaaaaccaa gtacgcggat caggcatgga tgcccgatcc aacccggcca
     9541 atgtttcaga cggcctgcaa aacagttcgg gccatatcgg taccaacacg cgctaccgcc
     9601 tgaccaagct cggcgaacaa atggcgcgcc tgcccatcga tccgaaaatt gcgcgcattt
     9661 tgctggcggc gaagaaacac gactgcatgg cggaaatatt ggtgattgcg tctgcgctgt
     9721 cgattcaaga cccgcgcgaa cggccgctgg aagcgcgcga tgccgcggcc aaggcgcatg
     9781 aacgctttac cgacaagcaa tccgatttcc ttacctacct gaatatttgg gacagtttcc
     9841 agcgcgagcg cgacaaaggt ttgtccaaca agcagttggt gcaatggtgc cgtcaatatt
     9901 ttctgtcgca cctgaggatg cgcgagtggc gcgagctgca ccaccagctt gcccaaaccg
     9961 cgattgaaat gggcttgacc accaaagaag ctgcgtttag acgacctccc gaagtcaagc
    10021 aactgacgtc gtctgagaat gcgggcgacc aagacctatc tgccaaactc aaacaaaaac
    10081 aactggataa gaaacaacac cgcacccaaa tccgcgctac caaagaagcg ggctacgaac
    10141 aaatccaccg cgccctgctc accggcctca tcgccaacgt cggcatgaaa tcgcccgaca
    10201 gcaacgacta caccggcgcg cgcggcagcc gcttccacct tttccccgcc tccgccctgt
    10261 tcaaagccaa gcccaaatgg gtgatggcgg cagaattggt tgaaaccacg cgcctttacg
    10321 cgcgcgacgt cgccgttatc cagcccgaat ggatcgagca ggaagccccg caccttgtcc
    10381 gctaccatta tttcgagccg cactgggaac aaaaacgcgg cgaagtcgtc gccagcgaac
    10441 gcgtaacgct ttacggcctg gccgtattgc cgcgccgccc cgtgtcttac ggtaaagttg
    10501 cgcccgaaga agcacgtgaa atctttatcc gcagcgcgtt ggtggcgcag gaatacgatt
    10561 tgaaagcgga tttttttgtc cacaacaaaa agctgattaa agaaattacc gaactcgaac
    10621 acaaatcgcg caagcaagac gtattggtcg atgacgaagc cctgtttgcg ttttatcacg
    10681 aacggctgcc cgatttttat atggcggatt cggtttcaga gggcctgtgt cctgcaaatc
    10741 cgcagcaaac caccccctcc cccgtggggg aaggctgggg agagggcaaa acagttgccg
    10801 cacaaaccaa cttttccgca acctcagcaa gccctctccc taaccctctc ccacaggaga
    10861 gggaacagag tgcctcagtt tcaacggttt caggcagtct gaaaacaatg tcttgcgaag
    10921 caaggctgaa tttctgcgaa gctaaaacta aaaccgaaag cagcctgcac tcccaaaggc
    10981 tatctgaaaa ctacaccccg ccattttcag acggcctgcg tcctgcaaac ccccagcaaa
    11041 ccaccccctc ccccgtgggg gagggctggg gagagggcaa aacagttgcc gcacaaacca
    11101 acttttccgc aaccgcagca aaccctctcc cacaggagag ggaacagagt gcctcggctt
    11161 taacagtttc agatgacccc aaacccaaaa agcagcctgc atctcaaaaa ggccgtctga
    11221 aacctttgcc ccttgccgat atccgcacct ttgaagcctg gctcaaaacc gccgagcgcg
    11281 acaacccgcg cctgttgttc ctcagccgcg acgacctcat gcagcacgcc gccgcgcaca
    11341 tcaccgaaga acaattcccc aaacactggc agaccgcaga cggcaaattc aaactcagct
    11401 accgcttcga gccgcaccac ccgctcgacg gcgttaccct caccctgccg ctcaccgtgc
    11461 tcaaccgcat cagccccgcc gccctcgaat ggctcgtgcc cggcatgata cgcgaaaaaa
    11521 tccagctaca aatcaaagca ctgcccaaac aaatccgccg catctgcgta cccgtacccg
    11581 aattcatcac ccaattttta agccaaaacc ccgaccgcaa cgcccccatc ctgccccagc
    11641 tcgcccaagc catcgccaaa accgcaggcg acatccgcat actcgagcaa atcaaccaag
    11701 acgaatgggc cgcgttcagg ctgcccgaac actgctattt caacctccgc atcatcgacg
    11761 acggcggaca agagttagcc atgggtcgcg atttaatcca aatccaacaa caacttggca
    11821 aagccgccac caccaccttc cgcgacaaca cccaagaatt cgagcgtgac aacgttaccg
    11881 catgggacat cggcatcctg cccgaatcca tcaaattcgc ccgcggcaaa caacagctca
    11941 ccggctacct cggcctgcaa aaagaaaaag acggccgcat cgccctgcgc ctgttcgaca
    12001 cgtctgccgc cgccggacac gcccacagat taggcgtgat tgaactcatg aaactacaac
    12061 taaaagaaca agttaaagat ctgaacaaag gcatccaagg cttcacccaa gccgccatgc
    12121 tgctcaaaca catcaacgcc gacaccctgc gcgacgacct cacccaagcc gtctgcgacc
    12181 gcgcctttat cggcgaagac aagctgccgc gcaacgaaaa agccttcaaa gaacaaatca
    12241 aacgcgcccg cagccgcctg cccgccgtca aagaagccct cagccgctac ctgcaggaaa
    12301 ccgcagccgc ctacgccgaa ctgaacggca aactcggcaa acacccattg acccacctcc
    12361 taagactacg cctgcaaacc ctgcttgcac ccggcttcgc cacccgcacc ccgtgggcac
    12421 aatggccgcg cctccccatc tacctcaaag ccatgaccct gcgcctcgaa aaatacagca
    12481 gcaaccccgc ccgcgacgca gcccgcgaag ccgatatcca agagctggaa caaatgtggc
    12541 aggaaaaaac tgacggcttg gcgaaacaag gacagcccgt ttcagacggc ctcgccgcgt
    12601 ttaaatggat gattgaagaa ttgagagtgt cgctgttcgc gcaggagttg aagacgccgt
    12661 atccggtgtc ggtaaagcgg ctgttgaaga tgtg
//
LOCUS       DAXDXW010000070        12403 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXDXW010000070 DAXDXW010000000
VERSION     DAXDXW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12403
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.071"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            101..1867
                     /locus_tag="ACFB64_001701"
                     /pseudo
     CDS             101..1867
                     /locus_tag="ACFB64_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            1971..2639
                     /gene="hda"
                     /locus_tag="ACFB64_001702"
     CDS             1971..2639
                     /gene="hda"
                     /locus_tag="ACFB64_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC1210019.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLQHKHDPFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     NMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            2636..3304
                     /locus_tag="ACFB64_001703"
     CDS             2636..3304
                     /locus_tag="ACFB64_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC1210020.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKLHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGIANMIGTQLETGPDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(3895..4791)
                     /gene="argB"
                     /locus_tag="ACFB64_001704"
     CDS             complement(3895..4791)
                     /gene="argB"
                     /locus_tag="ACFB64_001704"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC1210021.1"
                     /translation="MEFENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKETMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLVDTPEQNSVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDGLIADGTLYGGMLPKIASAVEAAANGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGRGEDA"
     gene            4961..6091
                     /locus_tag="ACFB64_001705"
     CDS             4961..6091
                     /locus_tag="ACFB64_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC1210022.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDMKI
                     WITARQHPGETMAEWFVEGLLGRLLDSQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRGKMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALEAQFKTALLNASPDFQDEYGYEKDAPGKANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAVLSAILNVAGDLR"
     gene            6165..7016
                     /gene="lgt"
                     /locus_tag="ACFB64_001706"
     CDS             6165..7016
                     /gene="lgt"
                     /locus_tag="ACFB64_001706"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC1210023.1"
                     /translation="MIIHHQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFSRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRITDINAFWA
                     MGFPQAHYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKP
                     RPTGQTAALFLGGYGVFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(7101..7763)
                     /locus_tag="ACFB64_001707"
     CDS             complement(7101..7763)
                     /locus_tag="ACFB64_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC1210024.1"
                     /translation="MDSRKFTEASKRRLGELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLAPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYDDGEG
                     GSDFYTWCNAYLYGLDIVPTDWFEAADDEAFEELFYPVMALGGIYDEEENGAIRLQFT
                     EGELAELASELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            7797..7903
                     /locus_tag="ACFB64_001708"
                     /pseudo
     CDS             7797..7903
                     /locus_tag="ACFB64_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7962..9755)
                     /locus_tag="ACFB64_001709"
     CDS             complement(7962..9755)
                     /locus_tag="ACFB64_001709"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC1210025.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKRHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGADIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGKAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSQIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNAESYKFISQKISTETGEEPRADIVFGIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVANGQGADTD
                     ILAATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            10001..11110
                     /gene="proB"
                     /locus_tag="ACFB64_001710"
     CDS             10001..11110
                     /gene="proB"
                     /locus_tag="ACFB64_001710"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFC1210026.1"
                     /translation="MKYKRIVFKVGTSSITRSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAIPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDSLAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            11123..12385
                     /locus_tag="ACFB64_001711"
     CDS             11123..12385
                     /locus_tag="ACFB64_001711"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC1210027.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADAEEKNRALLAMADSLEAAAEDI
                     LAANRLDLKAAAGKIPDSMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPEAVQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTGRPSVCNSMEVLLVHEGIAADFLPKLER
                     LLVRGRIEAGLPPVRFRLDPQAARYIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEARGTHHSDGIVTENRHAADYFTTHIDSAAVYINASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPIGLKELTSYKYIVQGTGQVRE"
BASE COUNT         2889 a         3589 c         3153 g         2772 t
ORIGIN      
        1 caagcaatat gccctgaccg gcaaaacttc cccaaacttc atcaaaaaat caactgtacc
       61 cgtctttttc agacggcatt gataccgtaa gagatttccc atgcaaaacc ggcaaaccga
      121 gctttattcc acgccgtctt ggctgttgca gaccttattg atgattaccg ccgcctcggc
      181 ggtgattctg tttttcgcgc gcaacacgcg cttggggcgg gagtttgcct atatcctgcg
      241 cctttgcctg acaccgaaaa gcacggtcaa agtattgctg ctgattacgg caatggttac
      301 gctgctgtta accgaagtgc ggttgaatgt gttgagtacc ttcatgtcca aaggacttta
      361 cgattcgatg caggatttga atgcttcggc attttggatg tttgcagcga tgaacgccgg
      421 cgtggtactg atacgggcgt ttaacaacgt cgttaacgac tttctcgatt aaggcttggc
      481 gattaaatgg tcggaacggc tcaatgaagt tttgacttcg cgctggcttg ccgacaaaaa
      541 ctactaccgc ctgcaaatgc gccgccacgc gccggacaac atcgaccagc gtatccaaca
      601 ggatgcgcag gaattcatcg cttcgaccat cgaatttgtg cgcggcatgg tcaattcggt
      661 cgttacttct ctggaatttg ccgttgtttt gtggggcttg gcaggcatcc tgaccgtgtt
      721 cggcttcgac attccgcacg gcattgtttg gtttgtcttt attttcatga ttttggcgat
      781 ctttatcgcc atgtggatag gcaacccttt gattcgttac aattatgaaa acaaaaaact
      841 caacggcgac taccgttatt ccctcatcct cgtacgcgac cacgccgaaa gcgtggcgtt
      901 ttacagcggc gaacaacacg aacacggccg gctttccgac cgctttaaag ccattatccg
      961 caaccgttgg cgcatcgcgc ggcaaagcgt ctgcttaagc ggctttaacg atatgttcac
     1021 caacggcatc aaactcttcc tgattatttt gcaagctccg cgcctgtttg ccggacaaat
     1081 caaaatcggc gacatccagc agaccgtcca agccttcgcg cgactgcaaa acgcgctgtc
     1141 tttcttccga atgttctaca acaaattcac cgcctgccac gcccgactgg agcgtctgta
     1201 cgtctttttg ctgagtaccg aagaacaaca cggcgcgcag caacccgaga ttagcgaagt
     1261 ttcagacggc attgcactgg aaaacgtcgc cctgttccgc cacaacggcg aagttttatt
     1321 ggacggcatc aacatcaaac tcaaaagcgg cgattccctg ctgatacgcg gcccgagcgg
     1381 tttaggcaaa acctcgctgc tgcgcgcgct ggcggggctt tggccgttcg gcagcagcgg
     1441 caaagtcagc cgtccgccgc atcaaggcat cctcttcctg ccgcaacgcc cgtacacggc
     1501 acaaggcagc ctgcgcgacg cggtttgtta ccccgatatc gacaaacagc atcccgagtt
     1561 agccgaagcc atgaacacct gccgcttggg ttatctgatt gacaaattgg ataaaacaga
     1621 cggctggcag cacaaactct cccccggcga actgcaacgc gtcgccttcg tccgcgccct
     1681 gctctccaag cccaaaatcg tcctgctcga cgaagccgcc gccgccttgg acgaaccggc
     1741 cgaagcctag ctctaccgcg ccttaaagca aaaactgccc gacagcatca tcagtatcgg
     1801 acaccgaagt acgctcaacg cgttccgcaa tatgcacctc gatgtcggca acgctgcctg
     1861 cggctgaacg atgccgtctg aagccgtttt cagacggcat cgttcaaatt ggaaagacac
     1921 gcgaaaacaa cgtacaataa acccgattcc aacccatccg gtatgccttt gtgaaccagc
     1981 ttattttcga ctttgccgca cacgactatc cgagtttcga caaattcctc ggcacggaaa
     2041 atgccgaact ggtctatgtg ttacagcata aacacgaccc gtttatctac gtctggggcg
     2101 aagaaggcgc gggcaaaagc catctgttgc aggcgtgggt cgcacaggcg ctcgaagccg
     2161 gcaaaaacgc cgcctatatc gatgccgcct ccatgccgct gaccgatgcc gcgtttgagg
     2221 cagaatacct cgccgtcgat caagtcgaaa aactgggcaa cgaagaacaa gccctgcttt
     2281 tttccatctt caaccgcttc cgcaacagcg gcaaaggctt tttgctgctc ggttcggaat
     2341 acacgcccca gcagctcgtc atccgcgaag atttgcggac gcgtatggca tactgcctcg
     2401 tttacgaagt caagccacta accgaccaag aaaaaatcga cgcgctcgcc aatatggcgg
     2461 cggcacgcca agtaaccgtc gattccgaaa ttttcgaata cctgctcaaa cactggcggc
     2521 gcgatatgga cagcctgatg atgatgctcg acacgctgga caactacgcc gtaaccatgg
     2581 gcaaacgcat caccctgccg ctgcttcgcc agcttttgaa acaacaggaa acccaatgaa
     2641 aaacctcgcc atcttcgacc tcgacaacac cctcatcaac accgattccg accacgcctg
     2701 gccgcaatac ctcatcaaaa aagggttggt tgacgctgcc gaaaccgaag cgcaaaacga
     2761 aaaattctac cgcgactacc aaaacggctg cctcgacatc gacgcattcc tcaaactcca
     2821 cctcgccccg ctcgcccgtt acagcaaaga agagctggcg gagtttcacc gcgaatttat
     2881 ggcggaatac atcatccccc acatctcgcc tatgcagcgt atgctggtgc agagccacca
     2941 aatggcaggc gacgaaaccc tcgtgatttc ctcaaccaac gagttcatca tcacccccgt
     3001 ctgccgcctt ttcggcatcg ccaacatgat cggcacacaa ctcgaaaccg gtcccgacgg
     3061 ccgctacacc ggcaattaca tcggcacgcc cagcctcaaa gaaggcaaaa tcacccgtct
     3121 gaaccaatgg ctcgccgaac gcggcgaaac gctcgaaagc tacggcaaaa cctattttta
     3181 cagcgactcc aaaaacgacc tgccgctgct gcgcctcgtc agcgaacccg tcgccgtcaa
     3241 ccccgatgcc gaactggaaa aagaagccaa agaaaaaggc tggccggttt tgaatttcaa
     3301 atgacgcaat gccgtctgaa gcccgattcg gcgttcagac ggcattgagg ctgaaatccc
     3361 aaaaacccaa agccacagga attcatccgg aaaaacaaaa aacacaggaa ttcatcggga
     3421 aaaacaacaa tctttccacc gtcattccca cgaaagtggg aatcttgatc cgtccgcacg
     3481 gaaacttata tttcgtcatt ccaccaatcc cacaccacct ttaccggttc aaatccgcac
     3541 aaaaatccac atcccgtcat tcccacgaaa gtgggaatct aggacgtaaa atctaaagaa
     3601 accttttttt cccgataagt ttccgtgccg acaggtctgg attcccgcct gcgcgggaat
     3661 gacgaaattt caaagttatg gcgttatcgg aaaaacagaa aatcaaagcc ggagaattta
     3721 tccaaaacaa ccggatttca aaaaaaccaa attcccggcg ggaatgacgg attttaggtt
     3781 tctgttgtgg tttctgtttg ctcgggaata acaacttaga aattgcccga aaccccaaaa
     3841 acagaaaccg gacaaacagg ttttccgcca aagccggcat tttccgactt tgcttcaggc
     3901 atcttcccct ctgcctaaaa tcatcgaccc gataccggca tcggtaaaga tttccagcaa
     3961 aagcgcgttg ggcaacctgc cgtcgatgat gtgcgtggct ttcacaccgt tggcggcggc
     4021 ttcgaccgca gaagcgattt tcggcagcat accgccatac agcgtgccgt cggcaatcag
     4081 cccatcaatc cgtttcggcg tgagtttggt cagcagattg cccgttttgt ccatcacacc
     4141 ggcgatattc gtcatcatca agagtttttc ggcgttcaat tcttccgcca atttccctgc
     4201 caccaaatcg gcgttgatgt tgaacgcttc gcctttttca cctacgccga cgggggcgac
     4261 gacgggaatg cagccgcgtt ctatcagccc tttaaccaaa ccggtatcga tgctttccac
     4321 cgtaccgacc tgtccgatgt ccacgctatt ctgttcgggc gtatcgacca aaagtttctt
     4381 cgccttaatg aaatggtcgt cgcgcccgct cacgccgacc gcgtgccctc catatgtgtt
     4441 aatcatcgac acgatttcct tgttgacgtg cccgcccaat accatttcga caatatccat
     4501 cgtctctttg tcggtaacgc gcattccttg gacaaattcg ccctttttgc cgactttttc
     4561 aagcatcgca ttgatctgcg gcccgccgcc gtgaacgatg acgggatgaa tgccgaccag
     4621 cttcagcagc acgacatcgc gggcaaaccc ttctttcaag gcaggttcgg tcatcgcgtt
     4681 gccgccatac ttgatgacgg cgaccgaacc ggaaaaccgg cggatgtaag gcagtgcttc
     4741 ggcaaggata cgcgccttgt cggcggcgga aataatgttt tcaaactcca taacggctcc
     4801 atattgttga caatttcggc ttatgttaac gccaaatgcc gtctgaaaca atcttcagac
     4861 ggcgtttggc acaccttcgg gcaggttgaa gccttgcgat acgggcggta aaatcgcggt
     4921 ttatccggtt cgagaaaccg gcgacagcaa ggactttccc atgattaaaa tcagcacccg
     4981 cttcgatgcc ggctcggtcg tcgtcaaaga cctgaccgac ccttccaaca tccgcctcgc
     5041 cctgcgtccc gacaacgcct ccgatttcgc acaatggttc tacttccggc tgcaaggcgc
     5101 ggcctatcaa aactgcatca tgcattttga aaacgcggca gaagccgcct acccgaaagg
     5161 ctgggaaggc tatcaggcgt gtgcctcata cgaccgccgc aactggttcc gcgtaccgac
     5221 ttcctacgaa aacggcgtac tgaccgtcaa tcatacgccg ctgtccaaca gcgtgtatta
     5281 cgcctatttc gaaccctatt ccgaagagca gcacctcaac ctcctcggcg acgcacaagg
     5341 cagcggcctg tgccgcatcg acgacttggg cagcaccgtg caaggccgcg acatcaatct
     5401 gctgaccata ggcaaccaag tcgaaagcga catgaaaatc tggattaccg cccgccagca
     5461 cccgggagaa accatggcgg aatggtttgt cgaagggctg ctcggcaggc tgctcgattc
     5521 gcaagacccg acagcacgca ccctgctcga ccgcgccacg ttctacatcg tccccaatat
     5581 gaatcccgac ggttcggcac tgggcaacct tcgtaccaac gccgccggtg cgaacctcaa
     5641 ccgcgaatgg gaaaacccga ctttggaaaa aagccccgaa gtgttcttcg tgcgcggaaa
     5701 aatgctggaa accggcgtgg atttgttttt ggacatccac ggcgatgaag gtctgccctt
     5761 tgtctttgtt gcaggaacgg aaggtgtgcc gaactacaat ccgcgcattt ccgcgttgga
     5821 agcgcagttc aaaaccgccc ttttgaacgc cagccccgat ttccaagacg aatacggtta
     5881 cgaaaaagac gcgccgggca aagcgaatat gaccttggcg accaactggg tcggcaaccg
     5941 tttcaactgc cttgcctaca cgctggaaat gccgttcaaa gacaacgcca acctgcccga
     6001 cgacgacttc ggctggaacg gccagcgttc tttgcggctt ggggaagcgg tgctttccgc
     6061 cattttgaac gtcgccggcg atttgcgctg acactcccca ctgaaaatgc cgtctgaaag
     6121 cccttttgcg tctttcagac ggcatttgaa aacgaaagcc cgttatgatt atccatcacc
     6181 aattcgatcc cgtcctcatc agtatcggcc cgcttgccgt ccgctggtat gccttaagct
     6241 acatcctcgg atttattctt tttacctttc tcggcagaag gcgcatcgcg caaggcttgt
     6301 ccgtttttac caaagaatcg ctcgacgact tcctgacatg gggcattttg ggcgtgattt
     6361 tgggcggacg cttgggctat gtcctgtttt acaaattctc cgactacctc gcccatccgc
     6421 ttgatatttt caaggtatgg gaaggcggaa tgtcgttcca cggcggcttt ttgggtgtag
     6481 ttattgccat atggttgttc agccgcaagc acggcatcgg cttcctcaaa ctgatggaca
     6541 cggtcgcgcc gctcgttccg ctgggtctcg cttcgggacg tatcggcaac tttatcaacg
     6601 gcgaactttg gggacgcatt accgacatta acgcattttg ggcaatgggc ttcccgcaag
     6661 cgcattacga agatgccgaa gccgccgcgc acaatccgct ttgggcagaa tggctgcaac
     6721 aatacggtat gctgccgcgt catccctcgc agctttatca gtttgccctt gaaggcatct
     6781 gcctgttcgc cgtcgtttgg ctgttttcca aaaaaccgcg cccgaccggg cagactgccg
     6841 cgctttttct cggcggctac ggcgtgttcc gctttattgc cgaatttgcg cgccaacccg
     6901 acgactatct cgggctgctg accttggggc tgtcgatggg gcaatggttg agcgtcccga
     6961 tgattgtttt gggtatcgtc ggctttgtcc ggttcggcat gaaaaaacag cactgaccgc
     7021 ctgtgaaaaa taccgtctga aacatggaaa tccggtttca gacggcattt ttacgtttgg
     7081 ttcagatgaa agcaaacgct tcaattctta ccgcaacacg ccttgtattt cctgccgctg
     7141 ccgcacggac agggatcgtt cctgccggtt ttttcccctt ccctgcggac ggtttgcggt
     7201 ttgttgatga ccgcctgcca gtagcggtag atgtctgcca gcgcgtaagg cagttcggac
     7261 gcaagttccg ccagctcgcc ttcggtgaat tgcaggcgga tagcgccgtt ttcctcttcg
     7321 tcgtaaatgc cgcccaatgc catcacgggg taaaacagct cttcaaacgc ttcatcatcg
     7381 gcggcttcaa accaatcggt cggcacaatg tccaaaccgt aaagataggc gttgcaccaa
     7441 gtgtaaaaat cgctgccgcc ctcgccgtcg tcgtagagcc acaaatcggg cagcttttta
     7501 tccgacatcg cggcggttgt ttccatcgcc attgccaaaa ccagccgttc gatttcggaa
     7561 cgttcggcgg cggtaaattg cgattcgtcg cccaacactt cgggcagcca gtcgagcggt
     7621 gccaatttgt ccggcccgct caacagcgcc gtcataaaac cttgaacctc gtcgcaacgc
     7681 atcgtgttgc cttgttcgct tttggcatcc aataattcgc ccaaccgccg tttggatgct
     7741 tcggtaaatt ttcgggaatc catcattttc cttttcaaat gggttttgcg ccctattata
     7801 gtggattaaa tttaaatcag gacaaggcgg cgagccgcag gcagtacaaa tagtacggca
     7861 aggcgaggca acgccgtacc ggtttaaatt taatccacta tatcgccgca atgccgtctg
     7921 aagccttttt atgcttcaga cggcatcgtc tgttttcacg ctcaaatcgt accgctgccc
     7981 tgcgccttcg gtttggcggc actaaattcc agcttgctca aagcggaaag gtaagctttg
     8041 gcggtcgccg ccaaaatgtc ggtatccgcg ccctgaccgt tggcgacgcg gttgccgcgc
     8101 gccaaacgga cgctggtttc gccttggctt tccgtacctt gcgtgacggc gttgacggaa
     8161 taaatctgca aagtcgcgcc gctttgcgcc acgctctcaa tcgctttgaa aattgcatca
     8221 acagggccgg agccggttgc ggaagcgcgt ttttcttcgc ctttgatgcc gaacacgatg
     8281 tcggcgcgcg gctcttcgcc ggtttcggtg ctgatttttt gagaaatgaa tttatagctt
     8341 tcggcattca tattgcccat ttcgtccgac accagcgcgt gcaggtcttc atcgaagatt
     8401 tcgcgttttt tgtcggcgag ttctttgaag cgtgcaaatg ccgcgttcag tgcctcttcg
     8461 ctttccaact cgatgcccaa atccgccagc ttggttttga aggcgttgcg gccggacaat
     8521 ttgcccaagc tcaatcggtt tgccgaccaa ccgaccgatt cggcagacat aatctcgtaa
     8581 gtttcgcggt gtttcagcac cccgtcctga tggatgcccg attcgtgcga aaaggcattc
     8641 gcgccgacaa tggctttatt gggctgcacg ggatagcccg taatggtgga caccagtttg
     8701 gacgaaggca cgatttgcgt ggtatcgatg ccggtttcca agccgaacaa gtcgtggcgc
     8761 accttcaacg ccatcacgat ttcttcaacc gaagcattcc ccgcgcgttc gcccaaaccg
     8821 ttaacggtac attccacctg acgcgcgccg ccttttaatg cggcaagcga attggcaacc
     8881 gccaagccca aatcgttgtg gcagtgtgcc gaccagacga ctttgccgcc gttgggcgtt
     8941 ttggcaatca gttcgcggaa aaattcttcg gttttgtacg ggatggaata gccgacggta
     9001 tcgggaatat tgatggtggt cgcgcccgct tcaatcaccg cgccgcagat ttcggcaagg
     9061 aaatcgattt gcgaacgcaa cgcgtcttcg caggaaaatt ccacatcgtc ggtgtattca
     9121 cgagcgattt ttaccgcttt gaccgccgcc tcaatcacct gcttcggctt catcttcagc
     9181 ttgtactcca tatggatggg gctggtggcg atgaaggtgt ggatgcgttt tttcggcgcg
     9241 ggcgcaacgg ccttacccgc ctgacggatg tcccgctcga tggcgcggga caatgaacag
     9301 accgttgatt tggtaatggt tttcgcaatc gcattgaccg cctcgaaatc gcccgggctg
     9361 gcggcggcaa aacccgcttc gatgatgtcc gcgcccaatt tttccaactg gcgggcgacg
     9421 cggatttttt cctctttggt catagcggcg ccgggcgatt gttcgccgtc gcgcagggtg
     9481 gtgtcgaaaa taataacgcg gttggcctgt gtcatttctg ttctctccag agattcgttt
     9541 ttttgtaaaa aagcattcaa atccagcggt cttccttgcg attcggcaag cgcggtcaat
     9601 ttttgcagtt gcgccaaagg cagcgagccg cgtgtgcgcc atttgtagat ggcggcggtc
     9661 gtcgcggcat tttcaggatc gtgccgtttc aacgcttcgg caagcgcgtt cacgccgccg
     9721 aaataggcga ccagacggtc tatatctaat tgcatggtag tccttgtcta aaataaatca
     9781 gcccgcagca gaaatatgga caggattata cgcattttgg acaaaacggc aatattaatt
     9841 tgaaaaaatc tcaataagtt gctataaatt ttattaattt tatacatttt gtctatttta
     9901 ttgcagatgc ggcgtgattg ttgacaattg cgacagaaca gggcaaattc ggcggtacaa
     9961 ggcggcaacg agacaggaca aaccaacaag gacggaaaca atgaaataca aaagaatcgt
    10021 atttaaagtc ggcacatctt cgattacccg ttcggacggc agcctctcgc gcggcaaaat
    10081 ccaaaccatc acccgccagc ttgccgcatt gcatcatgcg ggacacgagc tggtcttggt
    10141 gtcttccggc gcggttgctg cagggttcgg cgcgctgggt ttcaaaaaac gtccggtcaa
    10201 aatcgccgac aaacaggctt ccgccgccgt cgggcagggg ctgctgatgg aagaatatac
    10261 ggcaaacctg tcttcagacg gcatcgtgtc cgcacaaatc ctgctcagcc gtgccgactt
    10321 tgccgacaaa cgccgctacc aaaatgccgg cggcgcactt tccgtgctgc tgcaacgccg
    10381 cgcgattccc atcatcaatg aaaacgacac ggtttcggtt gaggagttga aaatcggcga
    10441 caacgacaca ttgagtgcgc aagtggcggc gatgatacag gcagacctct tggtgctgct
    10501 gaccgacata gacggtcttt acaccggcaa cccgaacagc aatcccgatg ccgtacggct
    10561 ggacaaaatc gaacacatca accatgaaat catcgaaatg gcgggcggct cgggttcggc
    10621 aaacggcacg ggcggtatgc tgaccaaaat caaagcggca accatcgccg ccgaatccgg
    10681 cgtaccggtg tatatctgtt cctcactcaa acccgattca ttggccgaag ccgccgaaca
    10741 tcaggcggac ggctcgtttt tcgtcccccg tgccaaaggt ttgcggacac agaagcaatg
    10801 gctggcgttc tattccgaaa gccggggcag cgtttatgtg gacgaaggtg cggaacacgc
    10861 tttgtccgaa caagggaaaa gcctgctgat gtcgggcatt gccggaatcg aagggcattt
    10921 ttcccgtatg gacaccgtaa ccgtgtacag caaggcaacc aaacagcccc tgggcaaagg
    10981 gcgcgtcctt ttcggctccg ccgccgccga agacctgctc aaatcgcgta aggcgaaagg
    11041 cgtgttcatc catcgggacg attggatttc catcacgccc gaaatacgcc tgcttctgac
    11101 cgaattttag aaaaggaaac ccatgtcaaa cacacaaaaa cagcttgccc ttgccaaagc
    11161 ggcaaaaaaa tccgtcaaca cggcggatgc ggaagaaaaa aaccgcgcgc tgcttgctat
    11221 ggcggacagt ctggaagcgg cggcggaaga tattttggcg gcaaaccgtt tggatttgaa
    11281 agcggcggca ggcaaaattc ccgacagcat gaccgaccgc cttttgttgg acggcaaacg
    11341 catttgcgcg atggcggacg gcatcagggc ggttgccgcg ctgcccgacc ccgtgggcga
    11401 aatactggaa acctcgactc tgcccaacgg cttggaaatc gtcaaaaagc gcgtggcgat
    11461 gggcgttatc ggcattattt acgaaagccg cccgaacgtt acttccgatg cggcggcttt
    11521 ggcactgaaa agcggcagcg cggtcgtact ccgcagcggc aaagatgcat ttcaatccgc
    11581 acgcgccatc gttgccgccc tgaaaacggg gttggcacaa acccgcatcg accccgaagc
    11641 cgtacagctc atcgaagaca cagggcgtga gggcagttac gaaatgatga gggcgaaaga
    11701 ttatctagac ctgctgattc cgcgcggcgg ggcagggctg atacgggcgg tggttgaaaa
    11761 tgccgtcgtg ccggtcattg aaacggggac gggcatcgtc cacatttata tcgacaagga
    11821 cgcggattgg gacaaggcac tccgtatcgt gtacaacgcc aaaaccggcc gcccgtccgt
    11881 gtgcaactcg atggaagtgc tgctggtgca tgaaggcatt gctgccgact tcctgcccaa
    11941 gctcgaacgg ctgttggttc gcggccgtat agaagccgga ctgccgcccg tccgcttccg
    12001 tttggatccg caggcggcgc ggtatatcgg cggcgaagcg gcgggtgcag acgatttcga
    12061 taccgagttt ttagactaca tcctcgccgt gaaaaccgtc gcttcggttg aagaggcagt
    12121 cgggcacatc gaagcccgcg gcacgcacca ttcagacggc atcgttaccg aaaaccgcca
    12181 cgctgcggac tatttcacga cccatatcga ttctgccgcc gtgtatatca acgcgtccac
    12241 gcgcttcacc gacggcggcg aattcggctt gggttgcgaa atgggcatct ccacgcaaaa
    12301 actccacgcc cggggcccga taggtttgaa agagctgacg agttacaaat acatcgtaca
    12361 aggcacggga caggttaggg aataatccga attcaaacgt ctg
//
LOCUS       DAXDXW010000071        11830 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXDXW010000071 DAXDXW010000000
VERSION     DAXDXW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11830
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.072"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..486
                     /gene="def"
                     /locus_tag="ACFB64_001712"
     CDS             <1..486
                     /gene="def"
                     /locus_tag="ACFB64_001712"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC1210028.1"
                     /translation="LQYPDERLHTVAKPVEQVDERIRKLVADMFETMYESRGIGLAAT
                     QVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDAVTRAERVK
                     VEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLKKRQKHT
                     I"
     gene            574..1500
                     /gene="fmt"
                     /locus_tag="ACFB64_001713"
     CDS             574..1500
                     /gene="fmt"
                     /locus_tag="ACFB64_001713"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC1210029.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     ADIIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGTAGEVLSCSADGLVVACGES
                     ALKITELQPAGGRRMNIAAFAAGRSIEAGAKL"
     gene            1579..2838
                     /gene="rsmB"
                     /locus_tag="ACFB64_001714"
     CDS             1579..2838
                     /gene="rsmB"
                     /locus_tag="ACFB64_001714"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC1210030.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAHIRAAHPQLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLAASCKKDDVAKYNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLAVEGIAAKALDEYAVTLEEAVPVNRL
                     PGFAEGLVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARRQEALLDALWQALKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            2804..3403
                     /locus_tag="ACFB64_001715"
     CDS             2804..3403
                     /locus_tag="ACFB64_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC1210031.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIAATRAEARITDG
                     GRLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPTIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            3403..5523
                     /locus_tag="ACFB64_001716"
     CDS             3403..5523
                     /locus_tag="ACFB64_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFC1210032.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVSFSAMLL
                     LVLSAVLARYVILLLKDRRNGVFGSQIAKRLSGMFTLVAVLPGLFLFGISAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALDLAADNAVSNAVPVQIDLIGTASLSGNMGSVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHQFDQPLPDKEHWEQIQQTGSVRSLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPIPENVAQDAVLIEKARAKYAELSYSKKGLQTFFLVTL
                     LIASLLSIFLALVMALYFARRFVEPILSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLDGLTTGVVVFDEKGRLKTFNKAAE
                     QILGMPLAPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVQVEYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIRAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDDQDAQILTRSTDTIIKQVAALKEMVEAFRNYARAPSLKLENQDLNALI
                     GDVLALYEAGPCRFEAELAGEPLMMAADTTAMRQVLHNIFKNAAEAAEEADMPEVRVK
                     SETGQDGRIVLTVCDNGKGFGKEMLHNAFEPYVTDKPAGTGLGLPVVKKIIGEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            5516..6793
                     /locus_tag="ACFB64_001717"
     CDS             5516..6793
                     /locus_tag="ACFB64_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFC1210033.1"
                     /translation="MRSSDILIVDDEVGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVVMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKHGAAQIETGPVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQTGITFIIGKAERCRVRVIASCSYAAGSDGISCEEKLAGLFSESVVRIPPLSMQHE
                     DIPFLIQGIACNVAESQKITPTAFSDDALAVLSRYDWPGNFEQLQNVVTTLLLEADGQ
                     EIGIGAASSALGRGVPADGTGHMACGFDFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            6884..8071
                     /gene="dprA"
                     /locus_tag="ACFB64_001718"
     CDS             6884..8071
                     /gene="dprA"
                     /locus_tag="ACFB64_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC1210034.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRSFGSAQNALSAPAEQV
                     APAVRHKHALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGGKGIPTVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPANKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            8097..8558
                     /locus_tag="ACFB64_001719"
     CDS             8097..8558
                     /locus_tag="ACFB64_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC1210035.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPAGSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            8636..10942
                     /gene="topA"
                     /locus_tag="ACFB64_001720"
     CDS             8636..10942
                     /gene="topA"
                     /locus_tag="ACFB64_001720"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC1210036.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPGNGFAMKYQLISRNAKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPDEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEESEDGKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRRWIPVMDKFWKPFIKQVE
                     EKEGIERAKFTTQELNETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLAYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRRGVEKVCPQKECGWKEQIEPPAPQE"
     gene            11163..11291
                     /locus_tag="ACFB64_001721"
                     /pseudo
     CDS             11163..11291
                     /locus_tag="ACFB64_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
     gene            11281..11825
                     /locus_tag="ACFB64_001722"
                     /pseudo
     CDS             11281..11825
                     /locus_tag="ACFB64_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         2893 a         3285 c         3380 g         2272 t
ORIGIN      
        1 ttgcaatatc ccgacgagcg tctgcacacg gtggcaaagc ctgtcgaaca agttgacgag
       61 cgcatccgga agctggttgc cgatatgttt gaaacgatgt acgaatcgcg cggcatcggg
      121 ctggcggcga cgcaggtcga tgtgcacgaa cgcgtggtcg tgatggattt gaccgaagac
      181 cgcagcgaac cgcgcgtgtt catcaacccc gtcatcgttg aaaaagacgg cgaaaccact
      241 tacgaagagg gctgcctgtc cgtaccgggc atttacgacg ccgttacccg cgccgaacgc
      301 gtcaaggtcg aggctttgaa cgaaaaaggc gaaaaattca cgctggaggc ggacgggctg
      361 ctggcgattt gcgtgcagca cgagttagat cacctgatgg gcatcgtgtt tgtcgaacgc
      421 ctttcccaac tcaagcaggg gcggattaag accaaactga aaaaacgtca gaaacatacg
      481 atttgattcg cttcgtcatg ccgtctgaac gctgcaaggc tttcagacgg cacagccttg
      541 tccgacaact ttacgcacac gcaggaacac gctatgaaag tcatctttgc cggcacgccc
      601 gattttgccg ccgccgcctt aaaagccgtt gccgccgccg gttttgaaat cccgctggtg
      661 ctgacccagc ccgaccgtcc gaaagggcgc ggtatgcaac tgaccgcccc gcctgtcaaa
      721 caagccgcgc tggaactcgg tttgcgcgtg gcacagcccg aaaaactgcg caacaacgcc
      781 gaagccctgc aaatgctcaa agaggtcgag gcggacgtaa tggtggttgc cgcgtacggc
      841 ttgattctgc cgcaggacgt gttggatacg ccgaaacacg gctgcctcaa tatccatgct
      901 tcgctgctgc cccgttggcg cggcgcggcg ccgattcagc gcgcgattga ggccggcgat
      961 gccgaaaccg gcgtgtgcat tatgcagatg gacatcggtt tggacaccgg cgatgtggtc
     1021 agcgaacacc gctacgccat ccaacctacc gatactgcca acgaagtcca cgacgcgctg
     1081 atggaaatcg gtgcggcggc ggttgttgcc gatttgcaac agcttcaaag caaaggccgt
     1141 ctgaacgcgg tcaaacagcc cgaagaaggc gtaacttacg cccaaaaact aagcaaagaa
     1201 gaagcgcgta tcgattggag cgaaagcgcg gacattatcg aacgcaaaat ccgcgccttc
     1261 aaccccgtgc ctgccgcgtg ggtcgaatat cagggcaagc cgatgaaaat ccggcgtgcc
     1321 gaagtggtgg cgcaacaagg tacggcaggc gaagtgttgt cctgttcggc ggacggtttg
     1381 gtcgttgcct gcggcgaaag cgcgctgaag attaccgaat tgcagcctgc cggcggcagg
     1441 cgtatgaata ttgcggcgtt tgcggcggga cggtctattg aagcaggggc gaagctgtaa
     1501 atcccttcag acggcattcc gatccgcaaa cgggaatgcc gtctgaaacc atcagttgaa
     1561 gaaagcgaat cacataatat gagtatggca cttgcccaaa aacttgccgc cgacagcatt
     1621 gcggcggttg ccgaagggcg caaccttcag gacgtgttgg cgcacatccg cgcggcgcat
     1681 ccgcagctga cggcgcagga aaacggcgcg ttgcaggata tcgcctacgg ctgccagcgt
     1741 tatttgggca gtttgaaaca tatgctcgcg cagatgctga aaaagcccat cgacaatccg
     1801 cagctcgaaa gcctgctttt ggcggcgttg taccaactgc attacacgcg caacgcgccc
     1861 cacgccgtgg tcaacgaagc tgttgaaagc atagccaaaa tcgggcgcgg gcagtaccgt
     1921 tcgtttgcca acgcggtttt gcgccgcttt ttgcgcgaac gtgaaaaatt ggcggcttcc
     1981 tgcaaaaaag acgatgtggc gaaatacaac ctgccgctgt ggtgggttgc ctacctgaaa
     2041 aaccattatc cgaaacactg gcacaacatc gccgccgcgc tgcaatccca tccgccgatg
     2101 acgttgcgcg tcaaccgccg gcacggcaac gccgaaagct atttggaaaa actggctgtg
     2161 gaaggtatcg cggctaaggc gttggacgaa tatgcggtta cgttggaaga agccgtgccg
     2221 gtaaaccgtc tgcccggttt tgccgaagga ctggtgtcgg tacaggactt cggcgcgcag
     2281 caggcggcgt atctccttaa cccgaaagac ggcgaacgga ttttggacgc gtgcgccgcg
     2341 ccgggcggca agacggggca tattttggaa ctggcggact gccgcgttac cgccttggac
     2401 atcgatgcag gccgtctgaa gcgtgtggag gacaatatcg cccgtttggg ctttcagacg
     2461 gcatcggcgg cgtgtgccga tgcgcgggat ttggcggcgt ggtatgatgg aaagcctttc
     2521 gacgcaatcc ttgccgacgt gccgtgcacc gcttccggcg tggcgcggcg caatcccgac
     2581 gtgaaatggc tgcgccgacc gaccgacgcg ctcaaaaccg cccgccggca ggaagccctg
     2641 ctggacgcac tttggcaggc gttgaaacag ggcggcagaa tgctgcttgc cacctgttcc
     2701 gtgttcgtcg aagaaaacga cgggcaactg caaaaattcc tcaaccgcca tgccgatgcc
     2761 gaaccgatcg aatcgcgggt gcttttaccg aacaaacacc aagatggctt ttattacgcg
     2821 cttattcaaa agcattaaac aatggcttgt gctgttgccg atgctctccg ttttgccgga
     2881 cgcggcggcg gagggcattg ccgcgacccg cgccgaagcg aggataaccg acggcgggcg
     2941 gctttccatc agcagccgct tccaaaccga gctgcccgac cagctccaac aggcgttgcg
     3001 ccggggcgta ccgctcaact ttaccttaag ctggcagctt tccgccccga caatcgcttc
     3061 ttatcggttt aaattggggc aactgattgg cgatgacgac aatattgact acaaactgag
     3121 tttccatccg ctgaccaacc gctaccgcgt taccgtcggc gcattttcca ccgattacga
     3181 cactttggat gcggcattgc gcgcgaccgg cgcggttgcc aactggaaag tcctgaacaa
     3241 aggcgcgttg tccggtgcgg aagcagggga aaccaaggcg gaaatccgcc tgacgctgtc
     3301 cacttcaaaa ctgcccaagc ctttccaaat caacgcattg acttctcaaa actggcattt
     3361 ggattcgggt tggaaacctc taaacatcat cgggaacaaa taatgcgccg cttcctaccg
     3421 atcgcagcca tatgcgccgt cgtcctgctg tacggattga cggcggcaac cggcagcacc
     3481 agttcgctgg cggattattt ctggtggata gtctcgttca gcgcaatgct gctgctggtg
     3541 ttgtccgccg ttttggcacg ttatgtcata ttgctgttga aagacaggcg caacggcgtg
     3601 ttcggttcgc agattgccaa acgcctttcc gggatgttca cgctggtcgc cgtactgccc
     3661 ggcttgttcc tgttcggcat ttccgcgcag tttatcaacg gcacgattaa ttcgtggttc
     3721 ggcaacgaca cccacgaagc cctcgaacgc agccttaatt tgagcaagtc cgcactggat
     3781 ttggcggcag acaatgccgt cagcaacgcc gttcccgtac agatagacct catcggcacc
     3841 gcctccctgt cgggcaatat gggcagtgtg ctggaacact acgccggcag cggttttgcc
     3901 cagcttgccc tgtacaatgc cgcaagcggg aaaatcgaaa aaagcatcaa tccgcaccaa
     3961 ttcgaccagc cgcttcccga caaagaacat tgggaacaga ttcagcagac cggttcggtt
     4021 cggagtttgg aaagcatagg cggcgtattg tacgcgcagg gatggttgtc ggcaggtacg
     4081 cacaacgggc gcgattacgc gctgttcttc cgccagccga ttcccgaaaa tgtggcacag
     4141 gatgccgttc tgattgaaaa ggcgcgggcg aaatatgccg aattgagtta cagcaaaaaa
     4201 ggtttgcaga ccttttttct ggtaaccctg ctgattgcct cgctgctgtc gatttttctt
     4261 gcgctggtaa tggcactgta ttttgcccgc cgtttcgtcg aacccattct gtcgcttgcc
     4321 gagggcgcaa aggcggtggc gcagggtgat ttcagccaga cgcgccccgt attgcgcaac
     4381 gacgagttcg gacgtttgac caagctgttc aaccatatga ccgagcagct ttccatcgcc
     4441 aaagaagcag acgaacgcaa ccgccggcgc gaggaagccg cccgccacta cctcgagtgc
     4501 gtgttggatg ggttgactac cggtgtggtg gtgtttgacg aaaaaggccg tttgaaaacc
     4561 ttcaacaagg cggcggaaca gattttgggg atgccgctcg cccccctgtg gggcagcagc
     4621 cggcacggtt ggcacggcgt ttcggcacag cagtccctgc ttgccgaagt gtttgccgcc
     4681 atcggtgcgg cggcaggtac ggacaaaccg gtccaggtgg aatatgccgc gccggacgat
     4741 gccaaaatcc tgctgggcaa ggcgacggta ttgcccgaag acaacggcaa cggcgtggtg
     4801 atggtgattg acgacatcac cgtgctgata cgcgcgcaaa aagaagccgc gtggggtgaa
     4861 gtggcgaagc ggctggcaca cgaaatccgc aatccgctca cgcccatcca gctttccgcc
     4921 gaacggctgg cgtggaaatt gggcgggaag ctggacgatc aggacgcgca aatcctgacg
     4981 cgttcgaccg acaccatcat caaacaggtg gcggcgttaa aagaaatggt cgaggcattc
     5041 cgcaattacg cgcgcgcccc ttcgctcaaa ctggaaaatc aggatttgaa cgccttaatc
     5101 ggcgatgttt tggccctgta cgaagccggc ccgtgccggt ttgaggcgga acttgccggc
     5161 gaaccgctga tgatggcggc ggatacgacc gccatgcggc aggtgctgca caatattttc
     5221 aaaaatgccg ccgaagcggc ggaagaagcc gatatgcccg aagtcagggt aaaatcggaa
     5281 acggggcagg acggacggat tgtcctgacg gtttgcgaca acggcaaggg attcggcaag
     5341 gaaatgctgc acaatgcttt cgagccgtat gtgacggata agccggcggg aacgggactg
     5401 ggtctgcctg tagtgaaaaa aatcattgga gaacacggcg gccgcatcag cctgagcaat
     5461 caggatgcgg gtggggcgtg tgtcagaatc atcttgccaa aaacggtaga aacttatgcg
     5521 tagcagcgat attttaattg tagacgacga agtcggcatc cgcgacctgc tgtcggaaat
     5581 cctgcaggac gaaggctatt cggtcgcgtt ggcggaaaat gccgaagagg cgcgcaagct
     5641 gcgccatcag gcgcgcccgg caatggtgct gctggatatt tggatgcccg attgcgacgg
     5701 tatcaccctt ttgaaggagt gggcgaaaaa cgggcagctc aatatgccgg tggtggtgat
     5761 gagcgggcat gccagcatcg ataccgccgt agaagcgacc aaaatcggcg cgatcgattt
     5821 tttggaaaaa ccgatttccc tgcaaaagct gctgtctgcg gtcgaaaacg cgttgaaaca
     5881 cggtgcggcg caaatcgaaa cgggaccggt gttcgacaaa ctgggcaaca gtgccgcgat
     5941 tcaagagatg aaccgcgaag tcgaagcggc ggcaaagcgc acgtcccctg tatttttgac
     6001 gggcgaggcg ggttcgccgt ttgaaaccgt ggcgcgctat ttccataaaa acggtacgcc
     6061 gtgggtcagt ccggcaaggg tcgaatatct gatcgatatg ccgatggaac tgttgcagaa
     6121 ggcggagggc ggcgttttgt atgtcggcga catcgcccaa tacagccgca atatccaaac
     6181 cggcattacc tttatcatcg gcaaggcaga acgctgccgt gtcagggtga ttgcttcgtg
     6241 cagctacgcg gcaggttcag acggcatttc atgtgaagaa aaattggccg ggcttttttc
     6301 ggaatcggtc gtccgtattc cgcccctgag tatgcagcat gaagacattc ccttcctgat
     6361 acaggggatt gcctgcaatg tggcggaaag ccaaaaaatc acgcctaccg cattcagtga
     6421 cgatgcgctt gccgtattgt cccgttacga ctggccgggg aatttcgagc agttgcaaaa
     6481 cgtcgtgaca acgctgctgc tggaggcgga cgggcaggaa atcggtatcg gcgcggcttc
     6541 ttcggcactg gggcgcggtg tgcctgccga cggaacgggg catatggcgt gcgggttcga
     6601 ctttaacctg ccgctgcgcg aattaaggga ggaggtggag cggcgttatt tcgagtacca
     6661 catcgcccaa gaaggtcaga atatgagcaa agtggcgcag aaggtcggtt tggaacgcac
     6721 gcacctttac cgcaaactca aacagctcgg catcggcgtt tcgcgccggg cgggggaaaa
     6781 aaccgaagaa taggcccgga cggccggttt cccggctgcg ggcttttgtt ttcagacggc
     6841 atttggagaa aatgccgtct ggaatcgtaa ggggatggat tttatgacag aggacgaacg
     6901 tttcgcgtgg ctgcaattgg cgtttacgcc ctatatcggc gcggaaagtt tcctgctgct
     6961 gatgcgcagt ttcggcagcg cgcaaaatgc cttgtccgca ccggcggaac aggttgcccc
     7021 ggcggtgcgg cataagcacg cgcttgaggc ttggcgcaat gcggaaaaac gcgctttggc
     7081 gcggcaggcg gcagaagcgg cgttggaatg ggaaatgcgg gacggatgcc gcctgatgct
     7141 gcttcaggat gaagatttcc ccgaaatgct gacgcagggg ctgaccgcgc cgccggtttt
     7201 gtttttgcgc ggcaacgtgc ggctgctgca caagccttcc gccgccatcg tcggcagccg
     7261 ccatgccacg ccgcaggcga tgcggattgc caaagatttc ggcagggcgt tgggtgggaa
     7321 aggcattcct accgtgtcgg gtatggcttc gggcatcgac accgccgccc atcagggcgc
     7381 actggaggca gaaggcggca ccatcgccgt gtgggggacg ggcatagacc gcatttatcc
     7441 gccggccaac aaaaaccttg cctatgaaat cgccgaaaaa gggctgattg tcagcgagtt
     7501 ccccatcggc acgcggccgt atgccggcaa ctttccgcgc cgcaaccgcc tgattgccgc
     7561 gctgtcgcag gttacgctgg tggtcgaggc cgccttggaa tccggttcgc tgattaccgc
     7621 cggcctggcg gcagaaatgg ggcgcgaagt gatggcggta cccggttcga tagacaaccc
     7681 ccacagcaaa ggctgccaca aactgattaa agacggagcg aaactgacgg aatgtttgga
     7741 cgacatcctg aacgaatgcc cggggctatt gcaaaatacg ggtgcttcgt catattctat
     7801 aaataaggat acgcccgaca cgggccgccg cgccgttcag acggcatacg ccccgccgcc
     7861 tgccgcaaaa atgccgtctg aaggggcggc aggcggcaca gccgtcggcg gcatactgga
     7921 taaaatgggt ttcgacccca tccatcccga cgtgcttgcc ggacagttgg ctatgcctgc
     7981 cgcagatttg tatgccgcac tgttggaatt ggaattggac ggcagcgttg ccgcaatgcc
     8041 cggcggcaga taccagcgta tccgaacttg aacgcacttt atattaagga acacgaatga
     8101 ccgaagtcat cgcctacctc atcgaacatt tccaagattt cgatacctgc ccgccgcccg
     8161 aagacttggg tatgctgctt gaagaagcgg gtttcgatac gatggaaatc ggcaacaccc
     8221 tgatgatgat ggaagtattg ctcaacagct ccgaattttc cgccgaaccc gccggcagcg
     8281 gcgcattgcg cgtgtacagc aaagaagaaa ccgacaacct gccgcaggaa gtgatggggc
     8341 tgatgcagta tctgattgaa gaaaaagccg tcagctgcga acagcgggaa atcatcatcc
     8401 acgcgctcat gcacatcccc ggcgacgaaa ttaccgtaga taccgccaaa gtgctgaccc
     8461 tgctgctttt atgggcaaac aagagcgagc tgcccgtatt ggtcggcgac gagctgatga
     8521 gcgcgctttt actcgacaac aaacccacga tgaactgaag cggcttcaga cggcgccggc
     8581 ccgcccgatg ccgtctgaaa caccggcgtc aaaaccacca tccagagaac gacaaatggc
     8641 gaaaaacctc ttaatcgtcg aatccccgtc caaagccaaa accctgaaaa aatatttggg
     8701 cggcgatttt gaaatcctcg cgtcctacgg acacgtccgc gacctcgtcc ccaaaagcgg
     8761 cgcggtcgat cccggcaacg gctttgcgat gaaataccaa ctcatcagcc gcaacgccaa
     8821 acacgtcgat gccatcgtcg ccggagccaa agaagccgaa aacatctacc tcgcaaccga
     8881 cccggacagg gaaggcgaag ccatttcctg gcatcttttg gaaatcctca aatccaaacg
     8941 cggcctgaaa aacatcaagc cgcagcgcgt cgtgttccac gaaatcacca aaaacgccgt
     9001 gctcgatgcg gttgcccatc ctcgcgaaat cgaaatggat ttggtcgatg cgcaacaagc
     9061 ccgtcgcgct ctggactatc tggtcggttt caacctctcg ccgttgctgt ggaaaaaaat
     9121 ccgacgcggt ttgagcgcgg gccgcgtgca aagccctgct ttgcgcctga tttgcgaacg
     9181 cgaaaacgaa atccgcgcgt ttgaagcgca ggaatattgg acggtacatc tagacagcca
     9241 caaaggccgc agcaagttca ccgccaaact cgcccaatac aacggcgcga aactcgaaca
     9301 attcgacctg ccggacgaag ccgctcaagc cgacgtgttg aaagaactcg aaggcaaaga
     9361 ggctgtcgtt actgccattg aaaagaaaaa gcgcagccgc aaccccgccg cgccgttcac
     9421 cacatccacc atgcagcagg atgccgtgcg caaactcggc ttcaccaccg accgcaccat
     9481 gcgtaccgcc cagcagcttt acgaaggtat agacgtaggg cagggcgcca tcggtctgat
     9541 tacctatatg cgtaccgaca gcgtgaattt ggctgacgaa gcgttaaccg aaatccgcca
     9601 ttacattgaa aacaaaatcg gcaaagaata tctgccgagt gccgccaaac aatacaaaac
     9661 caaatccaaa aacgcccaag aagcccatga agcgatccgc ccgacttccg tgtaccgcac
     9721 gcccgaaagc gtcaaaccct tcctgagtgc agaccagttc aaactctatc aaatgatttg
     9781 gcagcgtacc gttgcctgtc agatgacgcc cgccaaattc gaccaaacca ccgtcgatat
     9841 taccgtcggc aaaggcgtgt tccgagtaac cggacaagtg caaaccttcg caggcttcct
     9901 cagcgtttac gaagaaagca gcgacgatga agagagcgaa gacggcaaaa aactgcccga
     9961 aatgagcgaa ggcgacaaat tgccggtcga caaactctac ggcgaacaac acttcaccac
    10021 cccgccgccg cgctacaacg aagccacact ggttaaagcc cttgaagaat acggcatcgg
    10081 ccgcccctcg acctacgcca gcatcatctc cacgctcaaa gaccgcgaat acgttaccct
    10141 tgagcaaaaa cgcttcatgc ccaccgacac aggcgacatc gtcaataaat tcctgaccga
    10201 acacttcgcc caatacgtcg attaccactt caccgccaaa ctcgaagacc agcttgacga
    10261 aatcgccgac ggcaaacgcc gctggattcc cgtgatggac aaattctgga aaccgttcat
    10321 caaacaagtg gaagaaaaag agggcatcga acgcgccaaa tttaccacgc aggaacttaa
    10381 cgaaacctgc ccgaaatgcg gcgaacacaa actgcaaatc aagttcggca aaatgggccg
    10441 cttcgtcgcc tgcgccggct atcccgaatg cagctacacg cgcaacgtca acgaaaccgc
    10501 cgaagaagcc gccgagcgca tcgccaaagc cgaggctgaa caagccgaac tcgacggacg
    10561 cgaatgcccc aaatgcggcg gtcggttagc gtataaatac agccgtaccg gcagcaaatt
    10621 catcggttgc gccaactacc ccaaatgcaa acacgtcgag ccgttggaaa aacccaaaga
    10681 taccggcgtc caatgcccgc aatgcaaaaa aggcaacctc gtcgagcgca aatcccgcta
    10741 cggcaaactg ttttacagtt gcagcaccta tcccgactgc aactacgcca cttggaaccc
    10801 gcccgttgcc gaagaatgcc cgaactgcca ttggccggtc ttgaccatca aaaccactaa
    10861 acgccggggc gtggaaaaag tctgtccgca aaaagaatgc ggctggaaag aacaaatcga
    10921 accgcctgca ccgcaagagt gaagcggttg ggattgagat gaaagaaaat gccgtctgaa
    10981 aggggtttca gacggcattt gtaaattaga aggggctgtc ccagatggct ggggaaattt
    11041 aaattaagtc agaattatcc ctatgagaaa agccgtccaa gccggtacaa acaaaagaaa
    11101 cccatcggac tgtttgccgc aggggtaact gcaagaacgg cagcagggtt ggcagcagtt
    11161 aatcaaaaca cggcagcctg ttattttcta tggcgaaata gtgtttggca catcatcggg
    11221 aaaacttcct ttacacgcat ttggacgaaa tctgcgaaat tatggaggca tacgacatat
    11281 cgtttacgat tacttatccg tcaaaacagt ccgcatttgg aaatgtatga cggcgaagca
    11341 ggggcgggtg aaagttattt ccgtaaaggc agacgcggcc gcagtgccgc cggcaaagcc
    11401 gccgtattcg ggcttttgaa gcgcaacggc aaggtttgta cggttactgt cggacactcg
    11461 aaccgctatt ttattgccta ttatccgcgg acgggtgaaa cccggcagca ttgtttatac
    11521 ggattgttcg tagttacgat gtatcagatt gtaagcggat ttggccgttt ccgtatcaat
    11581 cgcggcacac attttgccga acggcaaaac catatcaacg caattgggaa cttttggaat
    11641 cgggcaaacg tcatttgcgc aagtttgacg gcattcccaa agagcatttc gggctgtatt
    11701 tgaaggagtg cggacggcgt tttgacaaca gtggggtaaa agttcgaatt tccattttaa
    11761 aacaattggt aaagcaggat ttatcccggt tggccgggat tttaaaaaat aaatacattc
    11821 tttaacaaaa
//
LOCUS       DAXDXW010000072        11337 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXDXW010000072 DAXDXW010000000
VERSION     DAXDXW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11337
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.073"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(64..1143)
                     /gene="apbC"
                     /locus_tag="ACFB64_001723"
     CDS             complement(64..1143)
                     /gene="apbC"
                     /locus_tag="ACFB64_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC1210037.1"
                     /translation="MNIQNIRTLLETVAIPDTGRTIGGEKAVRSVGQRSDGIHIALHF
                     GFPVAHIAAALADAVQETLMPLTGDTHIHLSIDTEIGTHKVRPGVATIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVHDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            1362..1437
                     /locus_tag="ACFB64_001724"
     tRNA            1362..1437
                     /locus_tag="ACFB64_001724"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1395..1397,aa:Lys,seq:ttt)
     gene            1517..2026
                     /locus_tag="ACFB64_001725"
     CDS             1517..2026
                     /locus_tag="ACFB64_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC1210038.1"
                     /translation="MKKKLLSGIKFAVRTALVFLLVSLFLDWVRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVSDEDGGLARSWRIAATPAVVLVKNGKMVRYTTGISSYWGLRA
                     RIFQADFFG"
     gene            2367..2807
                     /gene="nadR"
                     /locus_tag="ACFB64_001726"
     CDS             2367..2807
                     /gene="nadR"
                     /locus_tag="ACFB64_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC1210039.1"
                     /translation="MPTQSKHASINIGLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVYLKLTSEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            2922..3422
                     /gene="hpaC"
                     /locus_tag="ACFB64_001727"
     CDS             2922..3422
                     /gene="hpaC"
                     /locus_tag="ACFB64_001727"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC1210040.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINMLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRPLD"
     gene            3438..4133
                     /gene="murU"
                     /locus_tag="ACFB64_001728"
     CDS             3438..4133
                     /gene="murU"
                     /locus_tag="ACFB64_001728"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC1210041.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIENALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVANGDVLTDIDFTAAFQTASSLPGHISAHLWLVGNPPHNPDGDFSLLPDGSVRPEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLLNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAAAWK"
     gene            complement(4167..4597)
                     /locus_tag="ACFB64_001729"
                     /pseudo
     CDS             complement(4167..4597)
                     /locus_tag="ACFB64_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            4772..6448
                     /locus_tag="ACFB64_001730"
     CDS             4772..6448
                     /locus_tag="ACFB64_001730"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC1210042.1"
                     /translation="MSFKTDAETAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RFGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIDNQPNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            6562..7653
                     /gene="ychF"
                     /locus_tag="ACFB64_001731"
     CDS             6562..7653
                     /gene="ychF"
                     /locus_tag="ACFB64_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC1210043.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNRFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERALLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVIAYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            7691..7930
                     /locus_tag="ACFB64_001732"
     CDS             7691..7930
                     /locus_tag="ACFB64_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210044.1"
                     /translation="MPSEAGFGGFRRSHTAGMQPHQNKIPPAFPICPPRFLQNKPPAL
                     PLREAVRYSEYPEPRYKMTFQTAFAQPAAFQVKTL"
     gene            7927..9801
                     /locus_tag="ACFB64_001733"
     CDS             7927..9801
                     /locus_tag="ACFB64_001733"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC1210045.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKDWLPGGFLGVDIFFV
                     ISGFLMTAILLREMSGGRFFLKTFYIRRIKRILPAFFAVLAATLAGGFFLFTKDDFFL
                     LWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVAR
                     KSLRVQFGFLAALCALSLAASFMPSALDKYYLPHLRACEMLLGSLTAVRMRYRQQRNP
                     AVGKRYAAVGALFSACILSACLFAYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLK
                     KFFQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAIVLTLAFSLISYH
                     CIEKPFKKWKGSFAQSVLWIYALPMLVLGAGSFFAMRLPFMAQYDRLGLTRSNTSCHN
                     NTGKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYA
                     SRVFQNWAACRAVYRYAEEHLPRYPKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRML
                     HKLSSEKQAVYLMADNLASSYNVQRAYILSSRIPGCRQTLRPDDESTLKANARIRELA
                     AKYPNVYIIDAAAYIPADFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHNH
                     "
     gene            9863..11158
                     /gene="tyrS"
                     /locus_tag="ACFB64_001734"
     CDS             9863..11158
                     /gene="tyrS"
                     /locus_tag="ACFB64_001734"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC1210046.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVGSIR
                     SQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIGVIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSRLTES
                     DFEQLALDGLPAFEVSDGINAVEALVKTGLAASNKEARGFVNAKAVLLNGKPAEANNP
                     NHAAERPDDAYLLIGEYKRFGKYTILRRGKRNHALLVWK"
BASE COUNT         2511 a         3104 c         3162 g         2560 t
ORIGIN      
        1 atgaagcccg ccggcaggcg cggggcttca gacggcattg ctgctgcggt ttcgggcgcg
       61 gctttattcg acgacaattt tagggaaccg gctgctgaag tctttgcctt tgtcggcaat
      121 gcccagggcg atttggaatg cggcatcggt gtagattcgg gcgatggcgg ggtgttcgtc
      181 gaacagttgc gccggtgtgc cgccgtccat ggcttcgcgc acgggcaggc ttaggggaag
      241 ctgtccgagc agggggacgt tgaggcgggc cgccaaatct ttgccgccgt ccgtgccgaa
      301 cagtgcttcg ctatgcccgc agttggagca gatgtggacg gacatgtttt ccaatacgcc
      361 caaaatggga atgttgacct tgcggaacat atccacagcc ttgcgcgcgt ctatcagcgc
      421 gatgtcctgc ggcgtggtta cgatgacgga cccggttacg gggatgcgtt gggacagcgt
      481 gagttggatg tcgcccgtgc cggggggcag gtcgatgaaa agatagtcca cttcgtccca
      541 ctcgctttgg aacatcagct gctgcaaggc ttggctgacc atcggcccgc gccagacgac
      601 ggcttggccg gtatcgacga gaaagccgat ggacataacc tgtatgccgt ctgaagattc
      661 gacgggaatg agtttttggt ttttctgatc gggtttgcgg tcgtgtacgc ccagcatcgt
      721 gggctggctc ggaccgtaaa ggtcggcatc gagcacgccg acgcgtgcgc ccatgcgcgc
      781 cattgcggcg gcaaggttgg cagtggtggt tgatttgccc acgccgcctt ttcccgatgc
      841 gacggcaatg atgtttttca cgcctttgat ggtggcgacg ccgggccgga ctttgtgtgt
      901 gccgatttcg gtgtctatgg acagatggat gtgtgtgtcg cctgtcagcg gcatcagggt
      961 ttcttgtacg gcatcggcga gggcggcggc gatgtgcgcg acagggaagc cgaaatgcag
     1021 ggcgatgtgg atgccgtctg aacgctgtcc gaccgagcgg acggcctttt cgccgccaat
     1081 cgtgcggccg gtgtccggga tggcgacggt ttcgaggagg gtgcggatgt tttgtatatt
     1141 catgatgtgt gcctctgttc gcaagtcgtg tgaggggaag atagacggaa agtgtacatg
     1201 attttgggca gtttgctttt ttcttgtgcg aacttttaca cggtatgcgg cggattgtga
     1261 aaatccgttg ccaaaatatc ccgtgcaaac agcagtctgc aattaagcct gtaaaaattg
     1321 cttgacggcg gggaacgggg ctgtataatg cacgacttat cgggtcgtta gctcagccgg
     1381 tagagcagcg gacttttaat ccgttggtcg agcgttcgaa tcgctcacga cccaccagat
     1441 aaccggaagc caagtttcga gacttggctt ttttgtttgc ctgccggttg cgcgggcgta
     1501 ccgtttgcgg gggtgtatga aaaagaaact gctttcgggc atcaaatttg ccgttcggac
     1561 ggcattggtg tttttgctgg tgtcgctgtt tttggattgg gtacgcaagc ccgacgaacc
     1621 tgccggggcg gcagggcggc ctttgaccct gctgtcgggg cagcggctga ctttgggaca
     1681 gtttagccgg gataggacgg tgctggtgta tttttgggga agctggtgcg gcgtgtgccg
     1741 ttatcagtcg ccgataatcg atgatttggc ggcggacggc gtgccggtcg tcggtgtggc
     1801 ggtacgttcc ggcagcgcgt ccgaagtggc ggcatatatg gcaaaacggg gcttgggctt
     1861 tccgactgtc agcgatgagg acgggggttt ggcaaggtct tggcggattg ccgcaacgcc
     1921 tgccgtcgtt ttggtcaaaa atgggaaaat ggtccgctat acgacgggaa tcagcagtta
     1981 ttggggcttg cgcgcacgaa tttttcaggc ggattttttc ggttaacttt tgtttttgtc
     2041 aaactttccg ctatgtaggg aatcaaacgg catatgcctt gcgcggcgga tgcggcggct
     2101 tttgtttttt gggaaagagc cggcgcgcct gccgatgtaa tcccttgttg tgattgtggg
     2161 aaaaatagat taaaatataa ctattaaaat attttcagat aggattatcg gaattaaagt
     2221 cttttatacc cggtcgtccg atgaggttta tagcgtattg ttgctatatg ttcgttttgt
     2281 tatataacgg ttgcatcaaa aattacgccc acaggctttc ccgacggttt gaaagtttga
     2341 ttttcgataa cttggagact taaacaatgc ctacccaatc aaaacatgcg tctatcaata
     2401 tcggtctgat acaggcaagg gaagccctga tgacccaatt caggcctatt ctgaatcagg
     2461 cgaatattac cgatcagcaa tggcggatta tccgtctttt ggcggaaaac ggcacattgg
     2521 attttcagga tttggcgaat caggcgtgca ttttgcgccc cagcctgacc ggtatcctga
     2581 cccgccttga aaaagcgggg ttggtcgtcc gcctgaaacc ttccaacgac caacggcgcg
     2641 tttatctgaa gctgacttcc gagggcgaga agctgtatga ggaaatcggc gaagaagtgg
     2701 acgagcgtta cgacgctatc gaggaagtgc tgggccgcga gaaaatgctg ctgcttaaag
     2761 acctgttggc agaacttgcc aaaatcgagg atgcgttgaa ctcgtaatac gccgtaatgc
     2821 gcggaaacgt ccgaccgacg gctttttgaa tcagaactgc tgcacatggg ggatgccttg
     2881 tgtgcagcat tcttatatag gggacggttt aaaggggaaa aatggcggat ttgcagaaaa
     2941 cttttcaaac ttcgttccgt gatgcgatgg catcctgtgc ggcaggcgtt catgtcatca
     3001 cgacagacgg tgcggcaggg cgttacggca ttacaatgac ggcggttgcg ccggttaccg
     3061 acgagccgcc gaccgtgatg ctgtgcataa accggagtgc gcgaatcatt ccgatcctgt
     3121 cggaaaacgg cagcctctgc atcaatatgc tggcggacga acatcaggat gttgccgaac
     3181 attttgccgg gctgaccggc ctgtcgcccg aagagcggtt tgcctaccat atttggcatc
     3241 gcggcaaaac gggacaactt gaaatagaag gcgcgttggc gcacctgcac gggcatattg
     3301 tcggcaaaca tgaaatcggc acgcattttg tgttttacgt caggctcgac gaaatcaaaa
     3361 actgcgggtg caaacgcccc gcgctgctgt atttcagacg gcagtttagg cctttagact
     3421 gatattcgga cagatatatg aaagcgatga tactggcggc aggacgcggc gagcgtatgc
     3481 gccctttgac cgacaccact ccgaagccgc tgctcgatgt ggcgggtaag cctctaatcg
     3541 gttggcactt gtgccgtctg aagcaggcgg ggtttaccga aatcgtcatc aaccacgctt
     3601 ggctgggtcg gcagatagaa aatgctttgg gcgacggctc ggcttatggc gtgaacatcg
     3661 cctattcgcc cgaacccgca ggcggtttgg aaacggcagg cggcatcgcg caggcattgc
     3721 cgctgttggg tgggcagccg tttttggtgg ccaacggcga cgtgctgacc gacatcgatt
     3781 ttaccgccgc gtttcagacg gcatcgtccc tgcccgggca catttccgca catttgtggc
     3841 tggtgggaaa tcccccgcac aaccccgacg gcgatttttc cctgctgccc gacggcagcg
     3901 tgcggccgga agtatccggc ggcaacggac tgacattcag cggcgtgggt atttaccgtc
     3961 ctgaaatgtt tgacggaatc gaagcgggca gtgtggcaaa actcgcgccc gtattgctga
     4021 acgaaatgcg gcaaaaccgc gtgagcggtc agaagcatac gggcctgtgg ctggatgtcg
     4081 gcacggtatg ccgtctgaag gaagcgcaag cccttgcagc ggcttggaag tgaaaactcg
     4141 gtttcaggcg gtatggcgga ttcggtttaa cgtttcagtg ccaacgtcaa cacgccgccg
     4201 taaccagccc caagtctatc cattcctgcg tgttcgggcg ttcgtctaag aaaatcaccg
     4261 ccatcagcgc gaccaagacc aggctgaatt tgtcgacggg cgcgacttgc ggggcgttgc
     4321 ccagttgcag ggctttgaag taggcgagcc aagatgcgcc ggtggcgagt ccggacaaaa
     4381 ccggaaacgt ccggttgcgc cccgtgaagc cgtttacgcc ctgccatttg ccggtgtagg
     4441 tcaaaaacaa taccaaggcg gcgaggatga ccaaggtgcg gataaaggcg gcgaaatccg
     4501 aatctatgcc ctgcaagccc attttggcga aaacggcggt caatgaggcg aagcctgccg
     4561 atgccaatgc ccaaaacagc catgcgttgc tgcccatgtt ttctcctttg attgtgaaca
     4621 atatgaacgg tatttttgtt gctgcgtcaa aaatttcact gcgggtttgg tgcggataac
     4681 gttataatat gcctgatatt attttcaatc cacctgtttg tcgcctgatg ctttcagacg
     4741 gcatgtccct cctcatttct aaaggaaaat catgagcttc aaaaccgatg ccgaaaccgc
     4801 ccaatcctcc accatgcgcc cgattggcga aattgccgcc aagctgggtt tgaacgttga
     4861 caacattgag ccttacggtc attacaaagc caaaatcaat cctgccgaag cgttcaagct
     4921 gccgcaaaaa caaggcaggc tgattttggt taccgccatc aacccgactc cggcgggcga
     4981 aggcaaaacc accgtaacca tcggtttggc ggacgcattg cgccatatcg gcaaagactc
     5041 tgtgattgct ttgcgcgagc cttctttggg tccggtgttc ggcgtgaaag gcggcgcggc
     5101 aggcggcggc tacgcgcaag ttttgccgat ggaagacatc aacctgcact tcaccggcga
     5161 cttccacgcc atcggtgcgg cgaataacct cctcgccgcc atgctcgaca accatatcta
     5221 ccaaggtaac gagttgaaca tcgaccccaa acgcgtgctg tggcggcgcg tggtcgatat
     5281 gaacgaccgc cagttgcgca acatcatcga cggtatgggc aagcctgttg acggcgtgat
     5341 gcgtcccgac ggcttcgaca tcaccgtcgc ctccgaagtg atggcggtat tctgccttgc
     5401 caaagacatc agcgatttga aagagcgttt tggcaatatt ctcgtcgcct acgccaaaga
     5461 cggcagcccc gtttacgcca aagatttgaa ggcacacggc gcgatggcgg cattgctaaa
     5521 agatgcgatt aagcccaatt tggtgcaaac catcgaaggc actccggcct ttgtacacgg
     5581 cggcccgttc gccaacatcg cccacggctg caactccgtt accgcaaccc gtctggcgaa
     5641 acaccttgcc gattacgccg taaccgaagc aggcttcggc gcggacttgg gtgcggaaaa
     5701 attctgcgac atcaaatgcc gccttgccgg tttgaaacct gatgcggcag tcgtcgtggc
     5761 gactgtccgc gccctgaaat acaacggcgg cgtggaacgc gccaaccttg gtgaagaaaa
     5821 cctcgaagcc ttggcaaaag gtttgcccaa cctgttgaaa cacatttcca acctgaaaaa
     5881 cgtattcgga ctgcccgtcg tcgttgcgct caaccgcttc gtgtccgact ccgatgccga
     5941 gttggcgatg attgaaaaag cctgtgccga acacggcgtt gaagtttccc tgaccgaagt
     6001 gtggggcaaa ggcggcgcgg gcggcgcgga tttggcgcgc aaagtcgtta atgccatcga
     6061 caaccaacct aataacttcg gtttcgccta cgatgtcgag ttgggcatca aagacaaaat
     6121 ccgtgcgatt gcccaaaaag tgtacggcgc ggaagatgtc gatttcagcg cggaagcgtc
     6181 tgccgaaatc gcctcgctgg aaaaactggg cttggacaaa atgccgatct gcatggcgaa
     6241 aacccaatat tcattgagcg acaacgccaa actcttgggc tgccccgaag gcttccgcat
     6301 caccgtacgc ggtatcactg tttccgccgg cgcgggcttc atcgttgcgt tgtgcggcaa
     6361 tatgatgaaa atgccgggcc tgccgaaagt tccggctgcc gagaaaatcg atgtggacga
     6421 acacggcgtg attcacggct tgttctgaac ggtttttgaa accggatgcc gtctgaagcc
     6481 gtttcagacg gcattttttc ggaacgcggg cggcggtatg ctataattct ccgttaaatt
     6541 tctctatttt caggaaaaac catgagtttg aaatgcggca tcgtcggttt gcccaacgtc
     6601 ggcaaatcca ccctttttaa cgcgctgacc caatcgggca tcgaagcggc aaactatccc
     6661 ttctgcacca tcgaacccaa cgtcggcatc gtcgaagtgc ccgacccgcg tatggcggag
     6721 ctggcgaaaa tcgtcaatcc gcaaaaaatg cagcccgcca tcgtcgagtt tgtcgatatt
     6781 gccggcttgg ttgcgggcgc gagcaaaggc gaaggcttgg gcaaccggtt ccttgccaac
     6841 atccgtgaaa ccgatgccat cgtcaacgtc gtgcgctgct ttgacgacga caacatcgtc
     6901 cacgtttccg gcaaagtcga tccgattgcc gacatcgaaa ccatcggcac cgaattggcg
     6961 cttgccgact tggcaagtgt ggaaaaagcc atcgtccgcg aagaaaaacg cgcccgatca
     7021 ggcgacaaag acgcgcaaaa actagtcgat ttgtgcaaaa aactgctgcc gcatctggac
     7081 gaaggcaaac ccgtgcgttc cttcggtttg gacgcggaag aacgcgcgct gctgaagccg
     7141 ctgttcctgc tgaccgccaa acctgcgatg tatgtgggaa acgttgccga agacggtttt
     7201 gaaaacaacc cgcacctcga ccgcctgaaa gaattggcgg caaaagaaaa cgcccccgtc
     7261 gttgccgtct gcgccgcgat ggagagcgaa atcgccgaat tggaagacgg tgaaaaagcc
     7321 gaatttctcg ccgaaatggg cttggaagaa ccgggcttga accgcctcat ccgcgcgggt
     7381 tacgaccttt tgggactgca aacctacttc accgccggcg tgaaagaagt ccgcgcgtgg
     7441 acgatacaca aaggcgacac cgcgccgcaa gccgccggcg tgatccatac ggattttgaa
     7501 cgcggcttca tccgcgccca agtgattgcc tacgatgatt ttgtctcgct cggcggcgaa
     7561 gccaaagcca aagaagccgg caaaatgcgc gtggaaggca aggaatacgt cgtgcaggac
     7621 ggcgacgtga tgcacttttt gtttaacgtg taacccaaat gcggcaggtt ttcagacggc
     7681 tttgccggaa atgccgtctg aagccggttt tggtggtttt cgacgttccc ataccgccgg
     7741 aatgcagccg catcaaaata aaatcccgcc cgcatttccg atttgccctc cccgattcct
     7801 gcaaaacaaa ccgcctgccc tgccgttacg ggaagccgtc cggtattccg aatatcccga
     7861 accccgatac aaaatgacct ttcagacggc atttgcacag cccgccgcgt ttcaagtaaa
     7921 aacattatga gccaagcctt accctaccgc ccggacatcg acacattgcg cgccgccgcc
     7981 gtcttgtccg tcatcgtgtt ccatatcgaa aaggattggc tgccgggcgg gtttctcggt
     8041 gtcgatatat tctttgtgat ttcaggcttt ttgatgacgg cgatcctcct tcgcgaaatg
     8101 tccggtgggc gtttcttcct caagacattt tatatccgcc gcatcaaacg gattttgccc
     8161 gcatttttcg ccgtattggc ggcaacgctg gcaggcggct tctttttatt caccaaagat
     8221 gatttctttc ttttgtggaa atccgcgctg accgccttgg gtttcgcctc caacctgtat
     8281 tttgcaaggg ggaaggatta tttcgatccc gcgcaggaag aaaagcccct gctgcacatc
     8341 tggtctttgt cggtcgaaga acaattttac tttgtctttc cgatattgct gttgcttgtc
     8401 gcccgcaaaa gcctgcgcgt acagttcggc ttcctcgccg cattgtgcgc cttaagcctt
     8461 gccgcttcct ttatgccttc cgcgctcgat aaatattacc tgccccacct gcgcgcctgc
     8521 gaaatgctgc tcggatcgct gaccgccgtg cggatgcggt accggcaaca gcggaatccc
     8581 gccgtcggga aacggtatgc cgccgtcggc gcattgtttt ccgcgtgcat actgtccgcc
     8641 tgcctgtttg cctattcgga acaaaccgcc tatttcccgg gccccgccgc cctgattccc
     8701 tgtctggctg ttgccgcgct gatttatttc aaccattacg aacacccgct taaaaaattt
     8761 ttccaatgga aaatcaccgt tgccgccggt ttgatttcct attcgcttta tctgtggcat
     8821 tggccgatat tggcctttat gcgctatatc ggcccggaca acctgccgcc ttattcgccg
     8881 gcggcagcga tcgtcctgac cctggcgttt tccctgattt cttatcactg catcgaaaag
     8941 ccgtttaaaa aatggaaagg ctcgttcgca caatccgttt tatggattta tgccttgcct
     9001 atgctcgttt tgggggcggg ctcgtttttc gcgatgaggc tgccgtttat ggcgcaatac
     9061 gaccgcttgg ggctgacgcg ttccaacacc tcctgccaca acaataccgg caaacaatgc
     9121 ctgtgggggg atacggaaaa acagccggaa ctgctggttt tgggcgactc ccacgccgac
     9181 cattacaaaa cattcttcga tgccgtgggc aaaaaagaaa aatggtccgc cactatggtt
     9241 tccgccgacg cctgcgccta tgtggaaggc tacgcgtccc gtgtgttcca aaactgggcc
     9301 gcctgccgcg ccgtttaccg ctatgccgaa gaacacctgc cccggtatcc gaaagtggtt
     9361 ttggcgatgc gctggggcag ccagatgccc gaaaacagcc gctcccttgc ctacgatgcc
     9421 ggttttttcc aaaaattcga ccgtatgctg cacaaactct catccgaaaa acaagccgtt
     9481 tacctgatgg cggacaactt ggcttcgtct tacaacgtcc agcgcgccta tatcttgtct
     9541 tcacgcatac cgggttgccg ccaaacactg cgcccggacg acgaaagcac cctgaaagcc
     9601 aatgcccgca tcagggaatt ggcagccaaa taccccaacg tctatattat tgatgccgcc
     9661 gcctatatcc ccgcagattt ccaaatcggc ggattgccgg tttactcgga caaagaccac
     9721 atcaaccctt acggcggcac agaattggcg aagcgttttt ccgaaaaaca aaggtttctc
     9781 gatacgcgcc ataaccattg attcgcttaa atttgttaca atcggcggtt tgcaaaaccg
     9841 ctaatttttt tgaaagagac cgatgagcgt catccaagac ctgcaatcgc gcggccttat
     9901 cgcgcaaacc accgacatcg aagccttaga cgctttgctg aacgaacaaa aaatcgccct
     9961 ttactgcggt ttcgacccga cagccgacag cctgcacatc ggacacctgc tgcccgtatt
    10021 ggcattgcgc cgcttccaac aggcggggca tacgccgatt gcactggtgg gcggcgcgac
    10081 cggtatgatc ggcgacccca gcttcaaagc cgccgaacgc agcttgaatt ccgccgaaac
    10141 cgttgccggc tgggtaggaa gcatacgcag ccaattaacc cctttcttga gctttgaagg
    10201 cggaaacgcc gccattatgg cgaacaatgc cgactggttc ggcagcatga actgcctcga
    10261 cttcctgcgc gacatcggca agcatttctc cgtcaacgcc atgctgaaca aagaatccgt
    10321 caaacagcgc atcgaccgcg acggcgcagg catttccttt accgagttcg cctattccct
    10381 gctgcaaggc tacgacttcg ccgagttgaa caaacgccac ggcgcggttt tggaaatcgg
    10441 cggttccgac cagtggggca acatcaccgc cggtatcgac ctgacccgcc gcctgaacca
    10501 aaaacaagtg ttcggtctga ccctgccttt ggttaccaaa tccgacggta ccaaattcgg
    10561 caaaaccgaa ggcggcgcgg tgtggctgaa cgcgaaaaaa acctcgccgt accagttcta
    10621 ccagttctgg ctgaaagtcg ccgatgccga tgtgtataaa ttcctgaaat actttacctt
    10681 cctgtccatc gaagaaatcg gtgtcatcga agccaaagac aaggcaagcg gcagcaagcc
    10741 cgaagcgcaa cgcatcctcg ccgaagaaat gacccgcctg attcacggcg aagaagccct
    10801 tgccgccgcg caacgcattt ccgaaagcct gtttgccgaa gaccaaagcc ggcttaccga
    10861 aagcgacttc gagcagctcg ccctcgacgg cctgcctgca tttgaagttt cagacggcat
    10921 caacgccgtc gaagccttgg tcaaaaccgg cttggcagcg tccaacaaag aagcgcgcgg
    10981 ctttgtcaat gccaaagcgg ttctgctcaa cggcaaaccg gctgaagcca acaaccccaa
    11041 ccacgccgcc gaacgccccg acgatgccta tctgttgata ggcgaataca aacgtttcgg
    11101 caaatacacc atcctccggc gcggcaaacg caaccacgcg cttttggttt ggaaataatc
    11161 cgattgccgc agaaatgccg tctgaagctt tcagacggca tttttatcaa atgcaaaaca
    11221 ccctgcgcct gccgatatgt cgtcatttcc atgcaggcgg gaattcaaac ttgtccgcac
    11281 ggaaacttat cgggcaaaac ggtttcttca gttctacgtt ctagatgccc gctttcg
//
LOCUS       DAXDXW010000073        11045 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXDXW010000073 DAXDXW010000000
VERSION     DAXDXW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11045
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.074"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(25..1053)
                     /gene="trpD"
                     /locus_tag="ACFB64_001735"
     CDS             complement(25..1053)
                     /gene="trpD"
                     /locus_tag="ACFB64_001735"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC1210047.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMCEFASKVPLEDADGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNIAASLSDGISAAREGIDSGRAKAKKEEF
                     VGFTRQFA"
     gene            complement(1117..1707)
                     /locus_tag="ACFB64_001736"
     CDS             complement(1117..1707)
                     /locus_tag="ACFB64_001736"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC1210048.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRCNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVEAMRHFAGRLPIMGVCLGHQTIGEAFGGDVVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIERGTLPDCLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTERGHDMLNNFLVEFQNFKPQKI"
     gene            2118..4394
                     /gene="znuD"
                     /locus_tag="ACFB64_001737"
                     /gene_synonym="tdfJ"
     CDS             2118..4394
                     /gene="znuD"
                     /locus_tag="ACFB64_001737"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HFC1210049.1"
                     /translation="MAQITLKPIVLSILLINTPLLAQAHETEQSVGLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGAAYSDRRDRYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDGDGAHAHTHNGKPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYHHDEKAGDAVENFFNNKTHNARIELRHQPIGR
                     LKGSWGVQYLGQKSSALSAIPETVQQPMLIDNNVRHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNQPLPDLGAHRQTARSFALSGNWYFTPHHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLAAYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDPYGKRPFIAQDDQNAPRIPAARLGFHLKTSLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            complement(4934..5713)
                     /locus_tag="ACFB64_001738"
     CDS             complement(4934..5713)
                     /locus_tag="ACFB64_001738"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC1210050.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDVYLPVDAQAQVAIGDKVTGVKTVLARLPLTDS
                     QADPVSQAASVETAANPSAEQQQIEAAAAKIQAAVQDVLKD"
     gene            5867..8716
                     /gene="uvrA"
                     /locus_tag="ACFB64_001739"
     CDS             5867..8716
                     /gene="uvrA"
                     /locus_tag="ACFB64_001739"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC1210051.1"
                     /translation="MCNHHPRHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILGPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     HHYKFDVNVAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCEKSVTGQYLGGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGKVIAKGSPEEVAKVKGSYTGKYLKVVLK"
     gene            8734..9375
                     /locus_tag="ACFB64_001740"
     CDS             8734..9375
                     /locus_tag="ACFB64_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="HFC1210052.1"
                     /translation="MNNNFDRSKHIYHNEAFVELVKDAIRFFHGTPVHTLPPPKQFKG
                     AGVYAIYYIGNNPLYKQYADWNRLSYNAPIYVGKAVPKGWRQARNSDNALNQSTELFH
                     RLKEHSRSIAAVSDLDPSDFMCRFVIFEGAGSDMIGTIEAALIKLHKPLWNSCVDGFG
                     NHDPGKGRYEQAKSDWDVLHSGRVWADRLNGIPNSYESILENINTHLEIIKRK"
     gene            9379..10512
                     /locus_tag="ACFB64_001741"
     CDS             9379..10512
                     /locus_tag="ACFB64_001741"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1210053.1"
                     /translation="MKSLEIFSGAGGLAKGLELAGFQHASFIELNKDACNSLRSNFNP
                     KLVYQGDVADFDLSSQEGIEVIAGGPPCQPFSLGGKHLAHEDRRDMFPHAVRYVEYYR
                     PKAFIFENVKGLLRKSFADYFEYILLRLTYPNLGILQNEDWKGHLTRLKEIEFNLYKG
                     IKYKVSYQLLNAADYGVPQKRERVVIVGIRADLDIDWKFPKRTHSEDRLNWEKYVTGE
                     YWEKHNEPKRFNKDIAEKLQKKYGIFEPEKKPWQTVRDTLSDIPHPLGNHKITGHEYR
                     DGARIYPGHTGSGIDEPSKTIKAGGHGVPGGENMIRYDDGTVRYFTSYEAKLLQTFPE
                     EFVISGAWGEAMRQIGNAVPVKLSEILGKHLMGVLSEKSSLHN"
     gene            10820..11008
                     /locus_tag="ACFB64_001742"
     CDS             10820..11008
                     /locus_tag="ACFB64_001742"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210054.1"
                     /translation="MDVQKQYPFETVAVCVLPNHIHAIWTLPPDDADYSLLRRLIKTK
                     FSAYSPHTKNLGAVLDYP"
BASE COUNT         2914 a         3106 c         2566 g         2459 t
ORIGIN      
        1 ttgctttata tcaagtttgc cggcttaggc gaattgccgt gtaaaaccga caaactcctc
       61 ttttttcgcc ttcgccctac ctgaatcgat gccttcccgt gcggcagata tgccgtctga
      121 aagcgaagca gcgatatttc cggcatataa ggcggcggcg gtattaagca atacgatatc
      181 gcgcgccgcc ccttctttcc cgtccagtac ctcgttcatt ttcaacaaag attcctgagt
      241 attggcaact ttgatttcat ccaaattgcg gcgggtttcg ataccgaaat cttctgggcg
      301 gatgtcgtat tcgctgattt ttccgtcttt gagttcggca acgcgcgttt tgcccgtcag
      361 tgtaatttca tccaaaccgc cctccccgca aacgaccaaa acgtgttttg agccgagttg
      421 ttgcaagacc cgcgacaaaa tgccgcacaa atcggtgtgg aacacgccca aaagctggtt
      481 cggcgcgccc gcaggattgg ttaacggacc caatatgttg aaaatgcttc ggaaaccgag
      541 cgaacggcgc accggggcga catggcgcat ggcactgtgg tgattgggtg cgaacataaa
      601 ccctataccg gtctgcctga tactttgggc aatctgttcg ggagtcaggt tgaggtttgc
      661 gcccatttgc tccatcacgt cagccgcacc gctggaggaa gagaccgacc ggcctccgtg
      721 tttggcaacc tttgcgcctg ccgctgcagc aacaaacatc gaagtcgtcg aaatattgaa
      781 ggttttcgcg ccatccccgc ccgtaccgac gatatcgacc agcccgtccg catcctccag
      841 cggcactttt gacgcaaact cgcacatcac ggcggcggcg gcggtgattt cggaaacggt
      901 ttcaaccttg atacgcaagc cggtcaaaat cgccgcaatt tgttcgggcg gcacttttcc
      961 gctcatcatt tggcgcatca agtcggtcat ttcatcgtaa aacaactcgt tattgctgat
     1021 taatcgttcg atagcctgtt gcggtgtaat cattttttgt cctccgttca atattcggac
     1081 gaaaatgccg cccgaagggc ttcagacggc atcacgtcag attttttgcg gtttgaagtt
     1141 ttgaaattca actaaaaaat tgttcaacat atcatgtccg cgttcggtca agagggcttc
     1201 ggggtggaac tgcacgccct cgacggcata ttccttatgg cgcacgccca taatttcgcc
     1261 gtcttccgtc cacgccgtga tttccaagca atccggcagc gtgccgcgtt cgataacgag
     1321 gctgtgataa cgcgtacagg taaccggatt gggcaaaccc ttaaacatac ccttgcccga
     1381 atgggacacg ggcgacacct taccgtgcat caaggttttt gcccgtacca catctccacc
     1441 gaacgcttcg cctatcgtct gatgcccgag gcacacgccc ataatcggca gccggccggc
     1501 aaaatggcgc atggcttcta ctgaaatacc cgcctcctta ggggaacacg gtccgggacc
     1561 gataacgaga tattgcggat tcaatgcctc gatttcctcc aacgtaatat catcgttgca
     1621 gcgcacggcg acttcctgcc ccaattctgc gaaatactgg acgatgttgt aagtaaaact
     1681 gtcgtaattg tcgataaaca aaagcatttt gtgattaacc tattgattta taatttgttt
     1741 attattaact gcatttccga tacccccatc cataccccct tatatcttag cgtgcccgat
     1801 gcgccctcgt gaatctggcg cagagcctcg cgttctgaca agtacgccat tttgccacct
     1861 tttcgaccgt tcgggaacgt accgttttta tgccctcata aattttggtt atccgatagc
     1921 taacatatag cggcgttcgt gttcacatca ttaacttccc taaaattagg gttaatacaa
     1981 attaacgcca ttaaatatca caaccgtgtc atttgaaaaa cttttgccta tgccctctga
     2041 gtattctcaa aatcttatga tataaaaaat gcaatgttat ataataacaa acttttgaaa
     2101 tgcgacgaga agccaaaatg gcacaaatca cactcaaacc cattgtttta tcaattcttt
     2161 taatcaacac acccctcctc gcccaagcgc atgaaactga gcaatcggtg ggcttggaaa
     2221 cggtcagcgt cgtcggcaaa agccgtccgc gcgcgacttc ggggctgctg cacacttcga
     2281 ccgcctccga caaaatcatc tccggcgata ctttgcgcca aaaagccgtc aacttgggcg
     2341 acgctttgga cggcgtaccg ggcattcatg cctcgcaata cggcggcggc gcatccgctc
     2401 ccgttattcg cggtcaaacg ggcagacgga ttaaagtatt gaaccatcac ggcgaaacgg
     2461 gcgatatggc ggacttttct cccgatcacg ccattatggt agataccgcc ttgtcgcaac
     2521 aggttgaaat cctgcgcggg ccggttacgc tcttgtacag ctcgggcaat gtggcggggc
     2581 tggtcgatgt tgccgatgga aaaatccccg aaaaaatgcc tgaaaacggc gtatcgggcg
     2641 aagccggatt gcgtttgagc agcggcaatt tagaaaaact gacatccgca ggcatcaata
     2701 tcggactggg caaaaacttc gtgctgcata ccgaaggctt gtaccgcaaa tcgggcgatt
     2761 acgccgtacc gcgttaccgc aatctgaaac gcctgcccga cagccatgcc gattcgcaaa
     2821 cgggcagcat cgggctgtct tgggtaggcg aaaaaggctt tatcggcgca gcatacagcg
     2881 accgtcgcga ccgctacggc ctgcctgccc acagccacga atacgatgat tgccacgccg
     2941 acatcatctg gcaaaagagt ttgatcaaca aacgctattt gcagctttat ccgcacttgt
     3001 tgaccgaaga agacatcgat tacgacaatc cgggcttgag ctgcggcttc cacgacggcg
     3061 acggtgcaca cgcacacacc cacaacggca aaccgtggat agacctgcgc aacaaacgct
     3121 acgaactccg cgccgaatgg aagcagccat tccccggttt tgaagccctg cgcgtacatc
     3181 tgaaccgcaa tgactaccac cacgacgaaa aagcaggcga tgcagtagaa aacttcttca
     3241 acaacaaaac acacaacgcc cgtatcgagt tgcgccacca acccataggc cgtctgaaag
     3301 gcagctgggg cgtgcaatat ttgggacaaa aatccagcgc gctttccgcc attcccgaaa
     3361 ccgtccaaca accgatgttg attgacaaca atgtccgcca ttacagcttt ttcggtgtag
     3421 aacaggcaaa ttgggacaac ttcacgcttg aaggcggcgt acgcgtggaa aaacaaaaag
     3481 cctccatccg gtacgacaaa gcattgattg atcgagaaaa ctactacaac cagcccctgc
     3541 ccgacctcgg cgcgcaccgc caaaccgccc gctcgttcgc actttcgggc aactggtatt
     3601 tcacgccaca ccacaaactc agcctgaccg cctcccatca ggaacgcctg ccgtcaacgc
     3661 aagaactgta cgcacacggc aagcacgtcg ccaccaacac ctttgaagtc ggcaacaaac
     3721 acctcaacaa agagcgttcc aacaatatcg aactcgcgct gggctacgaa ggcgaccgct
     3781 ggcaatacaa tctggcagcc taccgcaacc gattcggcaa ctacatttac gcccaaacct
     3841 taaacgacgg acgcggcccc aaatccatcg aagacgacag cgaaatgaag ctcgtgcgct
     3901 acaaccaatc cggtgccgac ttctacggcg cggaaggcga aatctacttc aaaccgacac
     3961 cgcgctaccg catcggcgtt tccggcgact atgtacgagg ccgtctgaaa aacctgccgt
     4021 ccctacccgg cagggaagat ccctacggca aacgtccctt catcgcgcag gacgaccaaa
     4081 acgccccccg cattccggct gcgcgcctcg gcttccacct gaaaacctcg ctaaccgacc
     4141 gtatcgatgc caatttggac tactaccgcg tgttcgccca aaacaaactc gcccgctacg
     4201 aaacgcgtac gcccggacac catatgctca acctcggtgc aaactaccgc cgcaatacgc
     4261 gctatggcga gtggaattgg tacgtcaaag ccgacaacct gctcaaccaa tccgtttacg
     4321 cccacagcag cttcctctct gatacgccgc aaatgggccg cagctttacc ggcggcgtaa
     4381 acgtgaagtt ttaaaatcgg acaggcaaac aaaaaaggtt ttcagacggc attctgaaaa
     4441 cctttttttc tttttaatca acttgttagg aaatatggca aagacaagga acaaaccact
     4501 gtgccatcat tcccattttc tttgtttcat cattcttgct ttcacgccgt cattctcgca
     4561 aaagcagaaa aatcaaaagc aaaaacccaa gatcccatca ttcccgcgca ggcgggaatc
     4621 caattcattc ggtttcagcc gtttccgata aatcaccgta acgttaagtt cccggattcc
     4681 cactttcgtg ggaatgacgg cgtaaaggtt tctgtttttt aagttttagg taactaatga
     4741 gtcgtcattc ccgcgcaggc gggaatccaa ttcgttcggt ttctgttatt tctgataaat
     4801 caccgtaacg ttaagttccc ggattcccat tttcgtgaac ataacggtgt gaacattagc
     4861 aaaaattcaa aaaaccaaac ttcctcattc gtgtgaatga tggtactgat attttctatt
     4921 tcagttcgca aaattaatct ttcaacacat cttgcacagc cgcctgaatc ttagccgccg
     4981 ccgcttcgat ttgctgctgt tcggcagatg ggtttgccgc tgtttcaacc gaagcagctt
     5041 gtgaaacagg atcggcttga gaatcagtca gcggcaaacg cgccaatacg gtttttacgc
     5101 cggttacttt atcaccaatc gccacttgcg cctgcgcatc gacaggcaga tacacatcca
     5161 cgcgcgaacc gaagcggata aagccatagc gttcgccgcg ggacagtttc gcacctgctt
     5221 gggtgtaaca caaaatacgg cgtgccacca gaccggccac ttgaacaaaa gtaatttcac
     5281 gaccggaagc cgtagtcgcc aaaaccgcgt tgcgttcgtt ttccgtgctg gctttgtcca
     5341 aatccgcatt cacgaatttg cctttgttat agaccacttt cgttaccgta caatcggcag
     5401 gcgatttttg cgaatgcacg ttgaacacgt tcataaaaat actgattttc aaagcatcga
     5461 catcacgata cggatcgcgt gcgcgctcga ccaccacgat acggccgtca accggactca
     5521 acaccgcttc aggattttgc ggaatttcac gcgcagggtc gcggaaaaac tgcaatgcaa
     5581 atacggtaaa cacccaaaac ggcaaagacc accagccgca gcacatcgac accagcaggc
     5641 tcaaagccaa accgccgccg ataatcggcc agccctcacg ggcgataatc gggtggggat
     5701 aaagacggtt catttgtcat ccttttattc aaatatatta aatagcgaca ttataacgga
     5761 atccgccgcg ttccgccgac aaatgccgtc tgaacgcggc ttcagacggc atccgagaaa
     5821 gtgctacaat atcggttttc ctttattttt tcaggcggca tccattatgt gcaaccatca
     5881 tccccgacat tcgcacgaca acgacaccat ccgcattcgc ggcgcgcgca cgcataattt
     5941 gaaaaacatc gatttggaca ttccccgcca caagctcgtg gtggtaacag gattgtcagg
     6001 cagcggcaaa tcatcgctgg cgtttgatac tttatatgcc gaagggcagc ggcgttatgt
     6061 cgagagcctg tctgcctatg cgcggcagtt tttgcagatg atggacaaac ccgatgtcga
     6121 tttgattgaa ggcctgtcgc ctgcgatttc catcgagcag aaatccacca gccacaatcc
     6181 gcgttccacc gtcggcacgg taacggaaat ccacgactac ctgcgccttt tatacgcccg
     6241 tgtcggcacg ccgtattgcc ccgaacacaa gctgccgcta tccagccaaa ccgtatctca
     6301 aatggtcgat gccgtattaa aactgccgga agatacgcgc gtgatgattc tggggccggc
     6361 ggtgcgcgag cgtaagggcg agtttgtcga tttctttgcc gacttgcagg cgcaaggctt
     6421 tgcccgtgtg cgcgtggacg gcgaagtcta tcagcttgac gaagtaccga agctggaaaa
     6481 aaacatcaaa cacaatatcg acgtcgtcat cgaccgcgtg aaagtgaaag ccgacatcaa
     6541 acaacgactg gcggaaagtt ttgaaaccgc gctgcgccac ggcaacgagc gcgcgctggc
     6601 gatggaaatg gacagcggcg aagaacattg gttttccgcg cgttttgcct gccccgtatg
     6661 ctcatacagc ctgcccgaat tggaaccgcg cctcttttcg ttcaacaacc cgatgggttc
     6721 ctgcccgact tgcgacggct tgggcaacac caatttcttc gaccccgaaa aagtagtcgc
     6781 ccatcccgaa ttgtcgctgg caaccggcgc gattgacggt tgggacaaac gcaaccaatt
     6841 ctatttccaa atgattcaat cgctggcgca tcattataaa tttgatgtca acgtcgcttg
     6901 ggaaactttg cctgaaaaag tcaaaaaagt cgtgttgcac ggctcgggca aagaagtcat
     6961 tgatttcacc tacctgtccg aacgcggcac caccttcaac cgcagccacg ccttcgaggg
     7021 catcatcccc aatctcgaac gccgctaccg cgaaaccgac agcgaaaccg tgcgcgaaaa
     7081 actgcgcgag taccaaaatc accgtgcctg cccgagctgc ggcggcgcac gtttgcgcaa
     7141 agaagcacgc tacgtttacg tcggcggcga accgttgcac gaagtctccg cctggccgct
     7201 gaccaaaacc caccgattct tcgaaacgct ggatttggac ggcaacaaaa aacagatcgc
     7261 cgaaaaaatc ctcaaagaaa tcaccgagcg gctcggcttc ttgattaacg tcgggctgga
     7321 ttacctgaat ttaagccgtt ccgccgaaac cctttccggc ggcgaagccc agcgcatccg
     7381 cctcgccagc caaatcggca gcggcctgac cggcgtgatg tacgttttgg acgaaccctc
     7441 catcggcctg caccagcgcg acaacgaccg cctgctcgcc accctcaaac gcctgcgcga
     7501 tttgggcaac agcgtaattg tggtcgaaca cgacgaagac gccatccgcg aagccgattt
     7561 cgtcgtcgat atgggccccg gcgcgggcga acacggcggc aacgtactga ttgccgacac
     7621 acccgaaaat gtcgccaaat gcgaaaaatc cgttaccgga caatacctcg gcggcaaaaa
     7681 atccattgcc gtgccgtctg aacgcacgcc cgtcaatccc gaccgaatgc tcgtcctcaa
     7741 aggtgcgcgc ggcaacaacc tcaaaaacgt caccctcgag ttgccgctcg gtttgattac
     7801 ctgcatcacc ggcgtatccg gcagcggcaa atccaccctg attaacgaca ccctcgccaa
     7861 aatcaccgcc cgcgaactca accgcgccca agaagaaccc gccccatacg acgacatccg
     7921 cggcctcgaa cacctcgaca aagtcatcaa cgtcgaccaa tcccccatcg gccgcacccc
     7981 gcgctccaac cccgccacct acaccggcct gtttaccccc atccgcgaac tcttcgccgg
     8041 cgtacccctc tcgcgcgaac gcggctacaa cgtcggcaga ttctccttca acgtcaaagg
     8101 cggccgctgc gaagcctgcc aaggcgacgg cgtaatcaaa gtcgaaatgc acttcctgcc
     8161 cgacgtgtac gtcccctgcg aagtctgcca cggcaaacgc tacaaccgcg aaaccctcga
     8221 aatccaatac aaaggcaaaa acatcagcca agtcctcgac atgacggtcg aagaagcccg
     8281 cgaatttttc gacgccgtcc ccaccgtatc gcgcaaactg caaaccctga tggacgtagg
     8341 tctcggctac atccgcctcg gccaatccgc caccaccctc tccggcggcg aagcccagcg
     8401 cgtcaaactc gccttggaac tctccaaacg cgacaccggc agaacgctct acatcctcga
     8461 cgaacccacc accggcctgc acttcgccga catcgccctg ctgctggaag tcataggccg
     8521 tctgaaaggc aaaggcaact cgattgtgat tatcgagcat aatctggatg taattaaaac
     8581 cgcggattgg attgtggact tagggccgga aggcggcgat ggtgggggta aggttatcgc
     8641 gaaaggtagc cctgaggaag tggcgaaggt taaggggagt tataccggga agtatttgaa
     8701 agtggttttg aagtaaacga acggatatag aaaatgaaca ataattttga ccgcagcaag
     8761 catatctatc ataacgaagc ttttgtagaa ctggttaaag atgccatacg cttttttcat
     8821 ggtacgcctg ttcatacact tcctccaccc aaacagttca aaggtgcagg ggtttatgcg
     8881 atttactata tcggtaacaa tcctttgtac aaacaatatg cggactggaa cagattatcc
     8941 tacaatgcgc caatttatgt tggcaaggcc gtccccaaag gctggcggca agcaagaaat
     9001 tctgataatg cgctaaacca atcaaccgaa ctgttccacc ggttgaaaga acacagcagg
     9061 agtattgccg ctgtttctga tttagatcct tccgatttca tgtgcagatt cgttatcttt
     9121 gaaggtgccg gttctgacat gataggcaca atcgaagctg ccctgattaa attacacaaa
     9181 cctctatgga actcttgtgt cgatggtttc ggaaaccacg accccggaaa aggccgttac
     9241 gaacaagcca aatcggattg ggatgtcctt catagcgggc gggtttgggc agataggcta
     9301 aacggcatac cgaatagcta tgagtccata cttgaaaata tcaacacaca tttagagata
     9361 ataaagagaa aataacctat gaagtctttg gaaatttttt ccggcgcggg cgggttggca
     9421 aaaggccttg aacttgcagg attccaacat gcatctttta ttgaactcaa taaagatgca
     9481 tgcaattctt taagaagcaa ctttaacccc aagcttgttt atcagggaga tgtggcagat
     9541 tttgatcttt ccagtcaaga aggcatagaa gtcattgcag gcggtcctcc ttgccagccc
     9601 ttttcattgg gcgggaaaca ccttgcccat gaagacagaa gggatatgtt tcctcatgcg
     9661 gtcagatatg tggagtatta ccgccccaaa gcttttatct ttgagaatgt aaaaggactt
     9721 ttaagaaaat cattcgctga ttattttgaa tatatcctac ttcggcttac ttatccgaat
     9781 cttggtattt tgcagaatga agattggaaa ggccatctga cacggctgaa agaaattgaa
     9841 ttcaatttat ataaaggaat caaatataag gtttcatatc aattgttgaa tgcagctgat
     9901 tacggagtgc cgcaaaaacg cgaacgcgtg gttatcgtag gtatccgggc cgatttggat
     9961 attgattgga aattcccaaa aagaacacat tccgaagaca ggttgaattg ggaaaagtat
    10021 gtaacggggg aatactggga aaaacacaac gaacccaaaa gattcaataa agatattgct
    10081 gaaaagttac aaaaaaaata cggtatattc gaaccagaaa aaaaaccttg gcaaacggta
    10141 agggatacct tgtccgacat cccgcatcct ttggggaatc ataaaattac aggacatgaa
    10201 tatagggatg gcgcaagaat ttatcccgga cacacaggaa gcgggataga cgaaccgtcc
    10261 aaaaccatta aagcaggtgg gcatggcgtt cccggcggag aaaatatgat tcgttatgat
    10321 gatggaacag tcagatactt taccagctat gaagcaaaac ttcttcaaac attccctgaa
    10381 gaatttgtca tttctggagc ttggggagaa gcaatgcgac aaattggcaa tgcggttcct
    10441 gtcaaattgt cggaaatttt aggcaaacat ctgatggggg tgttgtcgga gaaaagcagc
    10501 ctgcacaact aacccgccca ctgcgtgtgt gcggtacgca cgcggttgct tgattgacgg
    10561 tttgccgttt caacttttaa cttcgttgaa gctagtgctt tcagacggcc tccaatgtcg
    10621 tttggaaaca tcaggtccaa caaagttaaa ccttaaacaa caaaccattc cttctccaac
    10681 acccaaacca caaacaaaaa tcctatggcg cgttatcgca gaaacttcat tgccggcggc
    10741 acattctttt tcaccgtcaa acccgccgcc ccgaaatcgc gtctgcttgt cggacatatc
    10801 ggcttcgcgt gcggcttata tggatgtgca aaaacaatat ccctttgaaa ccgtcgccgt
    10861 atgcgtgctg ccgaaccata ttcacgccat ttggacgctg ccgcccgacg atgcggatta
    10921 ttccctactc cggcggctga ttaaaaccaa attctccgca tattcccctc atactaaaaa
    10981 cttaggggct gtactagatt atccctaaat tccacaccga tcccgcagga tttttagctg
    11041 ccggg
//
LOCUS       DAXDXW010000074        10915 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXDXW010000074 DAXDXW010000000
VERSION     DAXDXW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.075"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            160..1239
                     /gene="hupA"
                     /locus_tag="ACFB64_001743"
     CDS             160..1239
                     /gene="hupA"
                     /locus_tag="ACFB64_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupA"
                     /protein_id="HFC1210055.1"
                     /translation="MKYKALPLLPLAAALAACAGGVAEPHVPVSIPTATPLPAGEVTL
                     SSDNGNIENINTAGAGSASDAPSRSRRSLDAAPQNTSGISIRQREVEKDYFGYKSKET
                     SFIFKTPGGAQYALSSYADPITVSYSSPDFKIPDRHAGQRLADGSRIFICCSDSGATS
                     YAEITKQDYMKFGAWIGPNGEIDLFAGGFPVGKTPPPAFSYGSSTPETALSKGKITYQ
                     VWGIRVRNGQFVTSSYTPPKSGSYYGTLANTPVLSFITANFNSNTLAGKILGNSDYGP
                     DVDIQNATITGPTFSGDATSGGKSGKLEGKFFGKFASTRSSEVSIGGKITFDGDRSLD
                     TVFGGVSYEKKLDDTSQDTNHLTKQ"
     gene            1267..3696
                     /gene="hupB"
                     /locus_tag="ACFB64_001744"
     CDS             1267..3696
                     /gene="hupB"
                     /locus_tag="ACFB64_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HFC1210056.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLANDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVEIKNDGYVFDPANPSPSRYLTYKATGVARSQPDPQEWVNKSTLFKLG
                     YNFNDRNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRTGVEYK
                     NELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDTNGINGEVYHSFRHIRQNTAQWTA
                     DFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYDPKILASNQAKITMLIENRS
                     KYKFAYWNNVFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAHA
                     GFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKTNPELKAEKAKN
                     WELGLAGSGKAGSFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSPV
                     WQNQNRTAAWVEGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINALS
                     PWTAVYSLGYDAPSKRWGVNAYAARTAAKKPSDTVHSNDDLNKPWPYAKHSKAYTLFD
                     LSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSVREFGTVNRVNNKTHAGIQRFTSPG
                     RSYNFTIEAKF"
     gene            complement(4589..5935)
                     /gene="mnmE"
                     /locus_tag="ACFB64_001745"
     CDS             complement(4589..5935)
                     /gene="mnmE"
                     /locus_tag="ACFB64_001745"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC1210057.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPEP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMEMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASGRSAARLALRSLKGDFSRRIHGLV
                     EGLITLRMLVEAALDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRMILDTLPSDLKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLCEAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            6373..10779
                     /gene="app"
                     /locus_tag="ACFB64_001746"
     CDS             6373..10779
                     /gene="app"
                     /locus_tag="ACFB64_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC1210058.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQARAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALAGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGWKYADLNKYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPHQNGKYFFND
                     NNNGAGKIDAKHKHYSLPYRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDKGKGELILTSNINQGAGGLYFEGNFTVSPKNNETWQGAGVHISDGSTVTWK
                     VNGVANDRLSKIGKGTLLVQAKGENQGSVSVGDGKVILDQQADDQGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDITTTGNNNNLDSKKEIAYNGWFGEKDATKTNGRLNLNYQPEEADRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWIDRTFKA
                     ENFHIQGGQAVVSRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTSCTEK
                     TITDDKVIASLSKTDIRGNVSLADHAHLNLTGLATLNGNLSAGGDTHYTVTRNATQNG
                     NLSLVGNAQATFNQATLNGNTSASDNASFNLSNNAVQNGSLTLSDNAKANVSHSALNG
                     NVSLADKAVFHFENSRFTGKISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSAADAPRRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSGKLKLAESSEGTYTLAVNNTGNEPVSLEQLTVVEGKDNTPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAGETEAALTAKQAQLAAKQQAE
                     KDNAQSLDALIAAGRNATEKAESVAEPARQAGGENAGIMQAEEEKKRVQADKDTALAK
                     QREAETRPATTAFPRARRARRDLPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVF
                     AVQDELDRVFAEDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVG
                     ILFSHNRTENTFDDGIGNSARLAHGAVFGQYGIGRFDIGISAGAGFSSGSLSDGIRGK
                     IRRRVLHYGIQARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRA
                     GIKADYSFKPAQHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAE
                     IKGFTLSLHAAAAKGPQLEAQHSAGIKLGYRW"
BASE COUNT         2933 a         3202 c         2625 g         2155 t
ORIGIN      
        1 ggtttttgtt aatccgctat aaaaaacaaa ggtagaaacg cggcaggcaa acgcaggcac
       61 gtccgatttc cgcgccgccc cataaaacag ttgacatcat atgacattta aattgataat
      121 caaaactatt attaatacat catcaagatt gagtatgcga tgaaatacaa agccctgccc
      181 ttactgccgc ttgccgccgc ccttgccgcc tgtgccgggg gggtagccga accgcacgtc
      241 cccgtgtcca tccccaccgc cacgccgctg cccgccggcg aggtaacgtt atcaagcgat
      301 aacggcaata tcgaaaacat caacaccgcc ggcgccggaa gcgcatccga cgcgccgagc
      361 cgcagcagac gctcgctcga tgccgccccg caaaacacat ccggcatttc cattcggcag
      421 cgggaagtag aaaaagacta tttcggctat aaatcaaaag aaacatcatt catcttcaaa
      481 acacccggcg gcgcgcaata cgccctttca tcctacgccg accccataac cgtcagctac
      541 tcctcccccg atttcaaaat tcccgaccgc cacgcagggc agcggctcgc cgacggcagc
      601 cgcatcttta tctgctgcag cgactccggg gctacttcgt atgcggaaat taccaaacaa
      661 gattatatga aattcggggc gtggataggt cccaacggcg aaatcgacct cttcgccggc
      721 ggcttccccg tcggcaaaac gccgcctcct gcgttctcct acggcagcag cacgcccgaa
      781 accgcattgt caaagggtaa aatcacttat caggtttggg gcatccgcgt cagaaacggg
      841 caatttgtta cttcctctta tacgccgccg aaaagcggtt cttattatgg gacacttgcc
      901 aatacccccg tcctttcctt tatcaccgcc aatttcaaca gcaacacact ggcaggcaaa
      961 atcctcggca acagcgatta cgggccggat gtggatattc aaaacgcaac gattaccggt
     1021 ccgacctttt ccggcgatgc cacgtccggc gggaaaagcg gcaaattgga aggcaagttt
     1081 tttggcaagt tcgccagcac ccgcagcagc gaagtcagca taggcggcaa aatcaccttt
     1141 gacggcgacc gctccctcga taccgtattc ggcggtgtga gttatgaaaa aaaacttgat
     1201 gacaccagtc aagataccaa ccaccttact aaacaataaa ccacctcatc cctgcaaagg
     1261 tttcttatgc ccattccctt caaacccgta ttggctgccg ccgccatcgc ccaagcgttt
     1321 cccgcctttg cggcagaccc cgcgccgcag tccgcccaaa cgctgaacga aatcaccgtt
     1381 accggcacgc acaaaaccca aaaactcggc gaagaaaaaa tccgccgcaa aaccttagac
     1441 aagctcttgg ccaacgacga acacgacctg gtgcgctacg accccggcat ttccgtcgtc
     1501 gaaggcggca gggcgggttc taacggcttt accatacgcg gcgtggacaa agaccgcgtc
     1561 gccatcaacg ttgacgggct ggcgcaggcg gaaagccgct cttccgaagc cttccaagaa
     1621 ttgttcggcg cgtacggcaa cttcaacgcc aaccgcaaca cttccgagcc ggaaaacttt
     1681 tccgaagtaa ccatcaccaa aggcgcggac tcgctcaaat ccggcagcgg cgcattgggc
     1741 ggcgcagtca attaccaaac caaatccgca agcgactatg tttccgaaga caagccctac
     1801 catttgggga taaagggcgg cagcgtcggc aaaaacagcc aaaaattcag cagcattacc
     1861 gccgccggca ggctctttgg tttggatgcc ttattggttt atacccgccg cttcggcaaa
     1921 gaaaccaaaa accgctcgac cgagggcgat gtcgaaatta aaaacgacgg atatgtcttt
     1981 gacccggcca acccctcgcc cagcagatac cttacctaca aagccacagg ggttgctcgc
     2041 tcccaacccg acccgcaaga atgggtaaac aaaagcaccc tgttcaagct gggctacaat
     2101 ttcaacgacc gcaaccgcat cggctggatt tttgaagact cgcgcaccga ccgttttacc
     2161 aacgagctgt ctaatttgtg gacgggtacg accacgtctg ccgcaacggg cgactaccgc
     2221 caccgccaag acgtgagcta ccgccgccgc accggcgtcg aatacaaaaa cgaattggaa
     2281 cacggcccgt gggacagcct caagctgcgc tacgacaagc agcgcatcga tatgaacact
     2341 tggacttggg acatcccgaa aaattacgat acaaacggca tcaacggcga ggtttaccat
     2401 tcgttccggc atatccgcca aaacaccgcg caatggactg ccgattttga aaaacaactc
     2461 gacttttcca aagccgtttg ggcggcgcaa tacggcttag gcggcggcag aggggacaat
     2521 gccaactcgg attacagtta tttcgcaaaa ctgtacgacc ccaaaatcct cgcttccaac
     2581 caagccaaaa tcacgatgct gatcgaaaac cggtcgaaat ataaatttgc ctattggaac
     2641 aatgtgtttc acttgggcgg caacgaccgc ttccgcctga atgcgggcat acgctacgac
     2701 aaaaacagca gcagcgcgaa agacgatccg aaatacacca ccgccatccg ggggcagatt
     2761 ccccatttgg gttcggaacg cgcgcacgcg ggcttcagct acggcacagg gttcgactgg
     2821 cggtttacca agcatctgca cttgttggca aaatacagca ccggcttccg cgcaccgact
     2881 tcggacgaaa cctggctgct gttcccacac cccgatttct acctgaaaac caatcccgaa
     2941 ctgaaagcgg aaaaagccaa aaactgggaa ttgggcctgg cgggcagcgg caaagctggc
     3001 agcttcaagc tttcgggctt caaaaccaaa taccgcgact ttatcgaatt gacgtatatg
     3061 ggcgtttcgt cagacgataa aaacaacccc agatacgccc cgctttcaga cggcacggca
     3121 ttggtcagct cgcccgtttg gcaaaaccaa aaccgcaccg ccgcctgggt ggaaggcata
     3181 gagtttaacg gcacttggaa cctcgacagc atcgggctgc ccaaagggct gcacaccggc
     3241 ctcaacgtca gctacatcaa gggcaaggca acgcaaaaca acggcaaaga aacgcccatc
     3301 aacgcgcttt cgccgtggac ggcggtttac agcctgggct atgacgcgcc ttccaaacgc
     3361 tggggcgtca acgcctacgc cgcgcgcacc gccgccaaaa agccgtccga caccgtccac
     3421 agcaacgacg acttgaacaa accgtggcct tatgccaaac acagcaaggc ctatacgctg
     3481 ttcgaccttt ccgcctacct caacatcggc aaacaggtta cgctccgcgc cgccgcgtac
     3541 aacattacca acaagcagta ctacacttgg gaatctttac gcagcgtccg cgagttcggc
     3601 acggtcaacc gcgtcaacaa caaaacccac gccggcatcc aacgctttac ctcgccgggc
     3661 aggagctaca atttcaccat cgaagcgaag ttctaagctc ctgatgtgcc aatgccgtct
     3721 gaaacccgat gatttgggtt tcagacggca ttttttacgg tcgcaccgcc gccccctttc
     3781 aggcttgtac caagcccaat cccgcaactc tccgtcattc ccgcaactct ccgtcattcc
     3841 cagggaagtg ggaatctaga aacgcaaagc cacaggaact tatccgaaac aacagcaacc
     3901 taaaattcgt cattcccacg aaagtgggaa tccagttcgt tgagtttcag ttatttaaaa
     3961 taaattttga aactttaatc ccatcattcc cgcgaaagcg ggaatccgga acgtaaaatc
     4021 taaagaaacc gttttatccg ataagtttcc gtaccgacgg ggctggattc ccgcctgcgc
     4081 gggaatgacg gcggagcggt ttctgttttt tccgataaat tcctaaaact taaaatttca
     4141 tcattcccac aaggacagaa aaccaaaaac agaaacctaa aattcgtcat tcccgcaaaa
     4201 gcgggaatcc agttcgttga gtttcagtta tttagaataa attttgaaac tttaatcccg
     4261 tcattcccgc gaaagcggga atccggaacg taaaatctaa agaaaccgtt ttcccgataa
     4321 gtttccgtgc cgacggggct ggattcccgc ctgcgcggga atgacgatat ttcagttttc
     4381 tgtttttgat ttttttgttt ttacaggaat gacggggctt tcagtattga aaaaagttgc
     4441 cgtaccgcgc cgataatttc cgcctgcgcg gaatgaagat ttaagcgttg cccgaaattc
     4501 aaaaaaccaa aaccgaacgg atcggattcc cgtttttatg ggataacgga atgttcagcc
     4561 ggacaccgcc acaatgcctc cggcccgctt attttccgat gcaaaacctc gaaaaaatca
     4621 cgccgagcag gtcgtccgcc gtaaattccc ccgtaatttc gccgcacgcg acctgagcca
     4681 gccgcaagtg ttcggcaaac agctcgattt gatggttgcc gcacaaagcg gccagcgaca
     4741 attcttcctg cgccgccttg agtgcgttga cgtgccgcgt ccgcgccaaa aacagccctt
     4801 cgctttcgcc ctgccaaccg gcctcgcaca acagcgtccg tttcagcgcg tccaagccgt
     4861 cgccggtttt cgccgacagc gcgatgacgg tttccgcgcc cgtaccgaac ccgcctgccg
     4921 cgtgtgcgtg caaatcggat ttgctgtgga tttcaatgcg tttcaaatcc gacggcaacg
     4981 tatccaaaat catccgcgtc ttttcattca aaccctcgcg cggatcgacc aacaccagcg
     5041 cgacatcggc ttcggatacg gctttgcggc tgcgttcgat gccgatgcgt tcgaccacgt
     5101 cgtccgtctc gcgcaaacct gccgtatcga caatatgcac cggcacgccg tcaatcagga
     5161 tacgttccct gaccgcgtcg cgcgtcgttc cggcaatatc ggtaacaatc gccacttcgt
     5221 cgcccgccag cgcgttcaac aggctggact tgccgacatt cggcgcaccg accaatacga
     5281 cattcagacc ttcgcgtaaa atcgcgccct gctgcgcgtt ggcaagcaca tcatccacgg
     5341 cgcggcgcaa gccgtccagt ttgccgcgtg cgtccgccgc ttcgagaaaa tcgatgtctt
     5401 cctcgggaaa atccagcgcc gcttcgacca gcatccgcaa ggtaatcaag ccttcgacca
     5461 aaccgtgtat ccgccgcgaa aaatcgccct tgagcgagcg caacgccaga cgcgccgccg
     5521 aacggccgga tgcgtcaatc aaatccgcca cgccttccgc ctgtgccaag tccagtttgt
     5581 cgtttaaaaa cgcacgtttg gtaaactcgc ccggctcggc aaggcgcgcg cccagttcca
     5641 aacagcggtt cagcagcatc tccatcacta ccggcccgcc gtgtccctga agctcgatga
     5701 catcttcgcc cgtaaaactt gccggcgcgg caaaaaacag tagcagcccg ctatcgattg
     5761 cctgtccgtc cgcgtccgta aaatcagcat aggttgccac gcgcggctcg ggcgttttcc
     5821 cacacaaagc ctgcgccatc ggcaacaggt ttttccccga tatgcgtatc acgcccacgc
     5881 cgccgcgtcc gggcgcggtc gcgactgccg caatcgttgg aacgttatcc gacataaaac
     5941 ccccgaaaat tcaaaacagc cgcgattata gcaaatgccg tctgaagtcc gacggttcgg
     6001 ctttcagatg gcattgccgg cttgtgtaaa acgatattcc gacaccgcac ggcacaaaac
     6061 cctgccccgc attccgcctc cggtcggttt ttaccgccga aaacggcggg gttccgaccg
     6121 aaaaggaacg gggataaagt aaagtgtgat atgatttcgt attcaaaatg atgcctttat
     6181 gtgcaaccca tcgattgctc cggcgagttg ataaaaatcc cctttttttc cttcccgccc
     6241 ttccctgcac catctccgaa cagaagcggc aaaatacgcc gaaccgcaca tatagccaca
     6301 tcccgcgcgc cgcccggaag cggaagccgc gccctcccaa acaaaccgaa tcccgtcaga
     6361 taaggaaaaa taatgaaaac aaccgacaaa cggacaaccg aaacacaccg caaagcccct
     6421 aaaaccggcc gcatccgctt ctcgcccgct tacttagcca tatgcctgtc gttcggcatt
     6481 ctgccccaag cccgggcggg acacacttat ttcggcatca actaccaata ctatcgcgac
     6541 tttgccgaaa ataaaggcaa gtttgcagtc ggggcgaaag atattgaggt ttacaacaaa
     6601 aaaggggagt tggtcggcaa atcgatgacg aaagccccga tgattgattt ttctgtggta
     6661 tcgcgtaacg gcgtggcggc attggcgggc gatcaatata ttgtgagcgt ggcacataac
     6721 ggcggctata acaatgttga ttttggtgcg gagggaagca atcccgatca gcaccgcttt
     6781 tcttaccaaa ttgtgaaaag aaataattat aaagcaggga ctaacggcca tccttatggc
     6841 ggcgattatc atatgccgcg tttgcacaaa tttgtcacag atgcagaacc tgttgagatg
     6901 accagttata tggatgggtg gaaatacgct gatttaaata aataccctga tcgtgttcga
     6961 atcggagcag gcagacaata ttggcggtct gatgaagacg aacccaataa ccgcgaaagt
     7021 tcatatcata ttgcaagcgc atattcttgg ctcgtcggtg gcaatacctt tgcacaaaat
     7081 ggatcaggtg gtggcacagt caacttaggt agcgaaaaaa ttaaacatag cccatatggt
     7141 tttttaccaa caggaggctc atttggcgac agtggctcac caatgtttat ctatgatgcc
     7201 caaaagcaaa agtggttaat taatggggta ttgcaaacag gcaaccccta tataggaaaa
     7261 agcaatggct tccagctagt tcgtaaagat tggttctatg atgaaatctt tgctggagat
     7321 acccattcag tattctacga accacatcaa aatgggaaat acttttttaa cgacaataat
     7381 aatggcgcag gaaaaatcga tgccaaacat aaacactatt ctctacctta tagattaaaa
     7441 acacgaaccg ttcaattgtt taatgtttct ttatccgaga cagcaagaga acctgtttat
     7501 catgctgcag gtggggtcaa cagttatcga cccagactga ataatggaga aaatatttcc
     7561 tttattgaca aaggaaaagg tgaattgata cttaccagca acatcaacca aggcgcgggc
     7621 ggtttgtatt ttgagggtaa ttttacggtc tcgcctaaaa acaacgaaac gtggcaaggc
     7681 gcgggcgttc atatcagtga tggcagtacc gttacttgga aagtaaacgg cgtggcaaac
     7741 gaccgcctgt ccaaaatcgg caaaggcacg ctgctggttc aagccaaagg ggaaaaccaa
     7801 ggctcggtca gcgtgggcga cggtaaagtc atcttagatc agcaggcgga cgatcaaggc
     7861 aaaaaacaag cctttagtga aatcggcttg gtcagcggca gggggacggt gcaactgaat
     7921 gccgataatc agttcaaccc cgacaaactc tatttcggct ttcgcggcgg acgtttggat
     7981 ttgaacgggc attcgctttc gttccaccgc attcaaaata ccgatgaagg ggcgatgatt
     8041 gtcaaccaca atcaagacaa agaatccacc gttaccatta caggcaataa agatattact
     8101 acaaccggca ataacaacaa cttggatagc aaaaaagaaa ttgcctacaa cggttggttt
     8161 ggcgagaaag atgcaaccaa aacgaacggg cggctcaatc tgaattacca accggaagaa
     8221 gcggatcgca ctttactgct ttccggcgga acaaatttaa acggcaatat cacgcaaaca
     8281 aacggcaaac tgtttttcag cggcagaccg acaccgcacg cctacaatca tttaggaagc
     8341 gggtggtcaa aaatggaagg tatcccacaa ggagaaatcg tgtgggacaa cgattggatc
     8401 gaccgcacat ttaaagcgga aaacttccat attcagggcg gacaagcggt ggtttcccgc
     8461 aatgttgcca aagtggaagg cgattggcat ttaagcaatc acgcccaagc agttttcggt
     8521 gtcgcaccgc atcaaagcca cacaatctgt acacgttcgg actggacggg tctgacaagt
     8581 tgtaccgaaa aaaccattac cgacgataaa gtgattgctt cattgagcaa gaccgacatc
     8641 agaggcaatg tcagccttgc cgatcacgct catttaaatc tcacaggact tgccacactc
     8701 aacggcaatc ttagtgcagg cggagacacg cactatacgg ttacgcgcaa cgccacccaa
     8761 aacggcaacc ttagcctcgt gggcaatgcc caagcaacat ttaatcaagc cacattaaac
     8821 ggcaacacat cggcttcgga caatgcttca tttaatctaa gcaacaacgc cgtacaaaac
     8881 ggcagtctga cgctttccga caacgctaag gcaaacgtaa gccattccgc actcaacggc
     8941 aatgtctccc tagccgataa ggcagtattc cattttgaaa acagccgctt taccggaaaa
     9001 atcagcggcg gcaaggatac ggcattacac ttaaaagaca gcgaatggac gctgccgtcg
     9061 ggcacggaat taggcaattt aaaccttgac aacgccacca ttacactcaa ttccgcctat
     9121 cgacacgatg cggcaggcgc gcaaaccggc agtgcggcag atgcgccgcg ccgccgttcg
     9181 cgccgttccc tattatccgt tacgccgcca acttcggcag aatcccgttt caacacgctg
     9241 acggtaaacg gcaaattgaa cggtcaggga acattccgct ttatgtcgga actcttcggc
     9301 taccgcagcg gcaaattgaa gctggcggaa agttccgaag gcacttacac cttggctgtc
     9361 aacaataccg gcaacgaacc cgtaagtctc gagcaattga cggtagtgga aggaaaagac
     9421 aacacaccgc tgtccgaaaa tcttaatttc accctgcaaa acgaacacgt cgatgccggc
     9481 gcatggcgtt atcagcttat ccgcaaagac ggcgagttcc gcctgcataa tccggtcaaa
     9541 gaacaagagc tttccgacaa actcggcaag gcgggagaaa cagaggccgc cttgacggca
     9601 aaacaggcac aacttgccgc caaacaacag gcggaaaaag acaacgcgca aagccttgac
     9661 gcgctgattg cggccgggcg caatgccacc gaaaaggcag aaagcgttgc cgaaccggcc
     9721 cggcaggcag gcggggaaaa tgccggcatt atgcaggcgg aggaagagaa aaaacgggtg
     9781 caggcggata aagacaccgc cttggcgaaa cagcgcgaag cggaaacccg gccggctacc
     9841 accgccttcc cccgcgcccg ccgcgcccgc cgggatttgc cgcaaccgca gccccaaccg
     9901 caaccccaac cgcagcgcga cctgatcagc cgttatgcca atagcggttt gagtgaattt
     9961 tccgccacgc tcaacagcgt tttcgccgta caggacgaat tggaccgcgt gtttgccgaa
    10021 gaccgccgca acgccgtttg gacaagcggc atccgggaca ccaaacacta ccgttcgcaa
    10081 gatttccgcg cctaccgcca acaaaccgac ctgcgccaaa tcggtatgca gaaaaacctc
    10141 ggcagcgggc gcgtcggcat cctgttttcg cacaaccgga ccgaaaacac cttcgacgac
    10201 ggcatcggca actcggcacg gcttgcccac ggtgccgttt tcgggcaata cggcatcggc
    10261 aggttcgaca tcggcatcag cgcgggcgcg ggttttagta gcggcagcct ttcagacggc
    10321 atcagaggca aaatccgccg ccgcgtgctg cattacggca ttcaggcaag ataccgcgca
    10381 ggtttcggcg gattcggcat cgaaccgcac atcggcgcaa cgcgctattt cgtccaaaaa
    10441 gcggattacc gatacgaaaa cgtcaatatc gccaccccgg gccttgcatt caaccgctac
    10501 cgcgcgggca ttaaggcaga ttattcattc aaaccggcgc aacacatttc catcacgcct
    10561 tatttgagcc tgtcctatac cgatgccgct tccggcaaag tccgaacgcg cgtcaatacc
    10621 gccgtattgg cgcaggattt cggcaaaacc cgcagtgcgg aatggggcgt aaacgccgaa
    10681 atcaaaggtt tcacgctgtc cctccacgct gccgccgcca aggggccgca attggaagcg
    10741 cagcacagcg cgggcatcaa attaggctac cgctggtaac cgccggatat gccgaaaggg
    10801 gtttgacgat gccgccgtgc actgtcaaac ccctttttct gccaccttgt gcctactcca
    10861 tcgtctgata accgtcaaat tgatttttag cccatgttcg gcgagtcggg gaaat
//
LOCUS       DAXDXW010000075        10539 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXDXW010000075 DAXDXW010000000
VERSION     DAXDXW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10539
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.076"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(67..1305)
                     /locus_tag="ACFB64_001747"
                     /pseudo
     CDS             complement(67..1305)
                     /locus_tag="ACFB64_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(1485..2858)
                     /locus_tag="ACFB64_001748"
     CDS             complement(1485..2858)
                     /locus_tag="ACFB64_001748"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC1210059.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGVNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKAEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYI
                     HRIGRTGRAGADGVAISLMDESEQKMFEAIKELTGNKLLIERIEGFEPRWWEQEGSKP
                     EKTETSEPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRRGHRTCALL
                     QPRYGVK"
     gene            3072..4076
                     /gene="dusB"
                     /locus_tag="ACFB64_001749"
     CDS             3072..4076
                     /gene="dusB"
                     /locus_tag="ACFB64_001749"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFC1210060.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRAFGAGWAVCEMLAS
                     DPTLRNTGKTLHRSDFADEGGIVAVQIAGSDPEQMADAARYNVGLGAQVIDINMGCPA
                     KKVCNVQAGSALMQDEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHQNLPAIAKIAED
                     CGIAALAVHGRARTQMYKGEARYELIAETKSRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECRAAILNHIRAMHAFYGETVGV
                     RIARKHIGWYIGEMPDGEQARREINRLDNAAAQYDTLAGYLERLAGKTDRWACGYREG
                     "
     gene            4089..4238
                     /locus_tag="ACFB64_001750"
     CDS             4089..4238
                     /locus_tag="ACFB64_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210061.1"
                     /translation="MPPFGFGRHLSAWFGGRAESRPANVENNKCRLKTSGTGFAKRLS
                     DGIPS"
     gene            4265..4504
                     /locus_tag="ACFB64_001751"
     CDS             4265..4504
                     /locus_tag="ACFB64_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC1210062.1"
                     /translation="MNPATADIAQCIEQNLNQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            4507..5043
                     /gene="ruvC"
                     /locus_tag="ACFB64_001752"
     CDS             4507..5043
                     /gene="ruvC"
                     /locus_tag="ACFB64_001752"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC1210063.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADEP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            5094..5963
                     /locus_tag="ACFB64_001753"
     CDS             5094..5963
                     /locus_tag="ACFB64_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFC1210064.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIAGLIGSLAYLLVKPRRRIGEIN
                     LAKCFPEWDEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAKCLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNNSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLQFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            6617..6751
                     /locus_tag="ACFB64_001754"
     CDS             6617..6751
                     /locus_tag="ACFB64_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210065.1"
                     /translation="MPSERIFRSVLRLVGVSSDTVKRKFAGAGLSYAGKAAGESLYCL
                     "
     gene            6867..7661
                     /locus_tag="ACFB64_001755"
     CDS             6867..7661
                     /locus_tag="ACFB64_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC1210066.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYDLPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAVSCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELVEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            7886..10489
                     /gene="pepN"
                     /locus_tag="ACFB64_001756"
     CDS             7886..10489
                     /gene="pepN"
                     /locus_tag="ACFB64_001756"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC1210067.1"
                     /translation="MSKTVRYLKDYQTPAYRILETELHFDIAEPQTVVKSRLTVEPQR
                     AGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEFSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDRFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRAGRA
                     VRRIENIRLLRQNQFPEDAGPTAHPVRPVSYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNVFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLMT
                     EAEQAFPLEGVTEAVVPSLLRGFSAPVYLNYPYSDDDLLLLLAHDSDAFTCWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGT
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELDRQAAKQENQSYEYSPETADWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNCLLAQFADKFSDD
                     ALVMDKYFALIGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAQD
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQELQCIRAQEGLSK
                     DVGEIVGKILG"
BASE COUNT         2416 a         2751 c         2994 g         2378 t
ORIGIN      
        1 tgaatgatga aataaaaatg ccgtctgaac ccgattggat tcagacggca ttttgtatgg
       61 caggatttat tcgctttcca actgaccgac ccattctgac aaggcagtca ggcgttgttc
      121 ggatttcgcc acgaacgggt tttcggtatc ccacgcgtag cctgccaaaa tcgaagaaag
      181 catacggctg atttccgggc tgcggtcggg cgcgtacacg acgttttgga agcggaaatc
      241 ataccagccg tccacatagg tgcggaacgc gtctacgccg atcatcaggg gttcggcaaa
      301 ttcggtttgc caatcggcgg cttcgctttt gagttgtttt gtcagcagat cggcagcaag
      361 ttcggcggag tgcagcgcga tggttacgcc cgacgagaac acggggtcga ggaactcggc
      421 ggcattgccc aacagcgcga aatgcctgcc gtgcagtgat ttgacgttgg cggaatagcc
      481 ttggatggag cggaacggaa aatcgttttc ccaaacggct ttgtccaaaa tttcgcttaa
      541 catcgggcat tcgtaaacaa attttttcaa caccgtttcc gattcgccgg caagtttgtc
      601 gggtgtgccg accacgccga cggaacagcg gttgtcgccg aagggaatca gccaaatcca
      661 cacgtcgcgg ttgttgcgga tgggtggtaa tcaggatttt gttgcggtcg aatttcgggt
      721 gggtaatgtt gtcgtcgatg tgcgtgaaat gcgtttggcg cggcggcagg tgcgagggcg
      781 tttccaagtt tagcaggcgc ggcagcacgc gtccgtagcc gcttgcgtcc aagacgaatt
      841 tcgcggtcag ttcatagctc tcgccggtgt cggtttcgat gttcaagcgg gcaaaatcgc
      901 cgctgttgtc gaacgcggtt acgccgtgcc cgaaacgtac ttcaacgcct tgtttggcgg
      961 cttcttcaat caggattttg tcgaacacgg cgcggcggac ttggtaaacc gtgccggggc
     1021 cgtctgaaaa tttatcggtg aaatcaaact cggtatagcg gctgccccac gaaaacgccg
     1081 caccgttttt caactgaaag ccgggcccgg tgcgaacggc atcggcaaaa ccggcttctt
     1141 ccagcatttc catacagtgc ggcagcaggc tttcgccgat gacgaagcgc ggaaagtgct
     1201 gttttccaac acgcagactt gataaccttt tttgcggagc agggcggatg cggccgaacc
     1261 tgccggacct gcgccgatta cggcgacatc gaattgggtg gacattggga aatcctgatg
     1321 gtttgagtgg aaaaggaaat gatattgtac aggcagcaaa tgccgtctga aactgtttgc
     1381 ggaagaaatt tatccggtgc gggcggttgc tcccaatgcg tcagtccggt ttgaaaaatg
     1441 ccgtctgaaa cgggaaatgt tcagacggca tttgattttc agggttattt tacgccgtaa
     1501 cgcggttgga gcagggcgca cgtccggtgt ccccggcggc ttcggcggct gcgcccggca
     1561 atttttccgc aggccgcgcc cgcatcgttt gccgtggttt ccggccgggt atttttttcg
     1621 cgctgagccc tggcggattc gtagcggttg cgttgtctcg gttcgcttgt ttcggttttt
     1681 tccggttttg agccttcctg ttcccaccat cggggctcga agccttcgat gcgttcgatg
     1741 agcagcttgt tgccggtcag ttctttgatg gcctcaaaca ttttctgttc ggattcgtcc
     1801 atcagggaaa tcgccacgcc gtccgcaccg gcgcgccccg tgcgtccgat gcggtggatg
     1861 tagtcttcgg gctgggcggg catttcgtaa ttgatgacga agggcagttc ggcgatgtcc
     1921 agcccacgcg cggcgatatc ggtggcgacg aggacgcgca ggctgccgtc tttgaaggcg
     1981 ttgagtgttt cgagcctgct ttgttgggaa cggtcgccgt gtatcgcctg tgcggacagg
     2041 ttgcggcgca ccagttcgcg cgttacgcgg tcgacgcttt gtttggtttt gcaaaagacg
     2101 ataacttggt tcatatgcag atcgacaatc agccgttcga gcaggttgcg cttttggaag
     2161 gtatcgacgg cgatgatgtg ttgttcgacg ttggcgttgg tggtgttttg ggcggcaacc
     2221 tcgacggttt cgggcgcgtt catgaagtct tgcgccagtt tgcgtatcgg ggcggagaag
     2281 gtggcggaaa agagcagggt ttggcgttgg cggggcagca tctgcatgat tttgcggatg
     2341 tcgtcgataa accccatatc caacatgcgg tctgcttcgt ccagaacgac gatttcggct
     2401 ttgtttaaac tgatgttttt ctgtttcaca tggtcgagca gccgtccgac ggtggcgacg
     2461 actatttcgc agccggcacg caggtcggcg gtttgtttgt ccatgttgac accgccgaag
     2521 aggacggtat gccgcagcgg caggttttta atgtagccct gcacgttttg gtcgatttgg
     2581 tcggcaagtt cgcgcgtcgg ggtcaacacg agcatacgca cggggtgcat tgcaggcgag
     2641 gtgctggcgg tggcgtaacg tttgaggcgt tccagactgg gcagcataaa ggcagcggtt
     2701 ttgcctgtgc cggtttgcgc ggcggcaagc aggtcgtgtc cggccagtgc tttgggaatg
     2761 gccgcggctt ggatgggcgt cgggttttcg taaccttgcg cggtcagtgc ggaaaccagt
     2821 tccgtaccca aacctaaaga ggaaaatgga ttactcatga ttgtagtctt tctttcagac
     2881 cttatgccgt ctgaagcggg aaaccgatag gacggggaaa taaactgccc gcctgtgaac
     2941 ggcggggacg ggaatttgtg tgcggattat gccataaaac ggtgcccgcc ccaatcgcgg
     3001 gcgcgtccgc agattggaaa tcctgcttaa aaaatgtaca atggcgcact tttttgaaac
     3061 gcgggcccat tatgcacatc ggcggttatt ttatcgacaa ccccatcgca cttgcgccga
     3121 tggcgggcat taccgacaaa cccttccgcc gcctctgtcg ggcgtttggc gcaggttggg
     3181 cggtgtgcga aatgctggcc agcgatccga cgctcaggaa taccggaaaa accctgcacc
     3241 gcagtgattt tgccgatgaa ggcggcatcg ttgccgtgca gattgccggc agcgaccccg
     3301 aacagatggc ggatgcggcg cgttacaacg tcggactcgg ggcgcaggtc atcgacatca
     3361 atatgggctg ccccgccaag aaagtgtgca acgtccaagc cggtagcgcg ctgatgcagg
     3421 acgagccgct ggttgccgcc attttggagg cggtggtcaa ggcggcgggc gtacccgtta
     3481 ccctcaaaac ccgtttgggt tggcacgacg accatcaaaa cctgcccgcc atcgccaaaa
     3541 tcgccgaaga ttgcggcatt gccgcccttg ccgtccacgg gcgcgcgcgc acgcaaatgt
     3601 acaaaggcga ggcgcgttac gaactcatcg ccgagaccaa aagccgtctg aacatcccgg
     3661 tctgggtcaa cggcgacatc acttcgccgc aaaaagccgc cgccgtcctc aaacaaaccg
     3721 ccgccgacgg catcatgata gggcgcggcg cgcaaggcag gccgtggttt ttccgcgatt
     3781 tgaagcatta tgccgaacac ggcgttttac cgcctgcctt gagtttggca gaatgcagag
     3841 ccgccatttt gaaccacatc cgcgccatgc acgcgtttta tggtgagacc gtcggtgtgc
     3901 gcatcgcacg caaacacata ggctggtaca tcggcgaaat gcccgacggc gaacaggcgc
     3961 ggcgtgaaat caaccgcttg gacaatgcgg cggcgcaata cgacacactt gccggttatc
     4021 ttgaaaggct tgccggaaaa accgaccgtt gggcgtgcgg ctatcgggaa gggtagggca
     4081 gtattgccat gccgccgttc gggttcggac ggcatctgtc tgcatggttc ggaggtcggg
     4141 cggaatcccg gccggcgaat gtcgaaaaca ataaatgccg tctgaaaacg agtggaacgg
     4201 gtttcgccaa aaggctttca gacggcattc cgtcttaaaa acatttcaat caaaaggata
     4261 tgcgatgaac cctgcgactg cggacattgc gcaatgtatc gagcagaact tgaaccaata
     4321 tttcaaagac ctgaacggca ccgaaccttg cggcgtgtac gatatggtac tgcatcaggt
     4381 ggaaaagccg ctgctggtgt gcgtgatgga gcaatgcggc ggcaaccagt ccaaagcctc
     4441 cgtgatgctg ggactgaacc gcaatacctt gcgtaagaaa ctgattcaac acggtttgct
     4501 gtgaatatgt cggcaaccgt ccgtatcttg ggcatcgacc cgggcagccg cgtaacgggt
     4561 ttcggcatca tcgatgtcag ggggcgcgat catttttacg tcgcctccgg ctgcatcaaa
     4621 acgcctgccg atgagcctct ggcagacagg attgccgtga ttgtgcggca tatcggcgaa
     4681 gtcgttgccg tttacaagcc gcaacaggcg gcggtggaac aggtgttcgt caacgtcaat
     4741 ccggcatcga cgctgatgct cggtcaggcg cgcggagcgg cattggcggc tttggtcagc
     4801 cataagctgc ccgtttcgga atacacggcc ttgcaggtca aacaggcggt ggtcggcaaa
     4861 ggcaaggcgg cgaaagaaca ggtgcagcat atggtggtgc aaatgctggg actttcggga
     4921 acgccgcagg cggatgcggc ggacggtctt gccgtcgcgc tgacccacgc cttacgcaac
     4981 cacgggcttg ccgccaaact caatccttcg gggatgcagg tcaagcgcgg aaggtttcaa
     5041 tagtttcaga cggcatttgt attttgccgc ctgaaaagaa aatgtgtacc gagatgaaat
     5101 ttatattttt tgtactgtat gttttgcagt ttctgccgtt tgcgctgctg cacaagattg
     5161 ccggcctgat cggttcgctt gcctaccttc tggtcaaacc gcgccgccgt atcggcgaaa
     5221 tcaatttggc aaaatgtttt cccgaatggg acgaagaaaa gcgtaaaacc gtgttgaaac
     5281 agcatttcaa acacatggca aaactgatgc tcgaatacgg cttatattgg tacgcgcctg
     5341 ccaaatgcct gaaatcgctg gtgcgctacc gcaataagca ttatttggac gacgcgctgg
     5401 cggcggggga aaaagtcatc atcctgtacc cgcactttac cgcgttcgag atggcggtgt
     5461 acgcgcttaa tcaggatgtc ccgctgatca gtatgtattc ccaccaaaaa aacaagatat
     5521 tggacgaaca gattttgaaa ggccgcaacc gctatcacaa cgtcttcctt atcgggcgca
     5581 ccgaagggct gcgcgccctc gtcaaacagt tccgcaaaag cagtgcgccg ttcctgtatc
     5641 tgcccgatca ggatttcgga cgcaacaatt cggtttttgt ggattttttc ggcattcaga
     5701 cggcaacgat taccggcttg agccgcattg ccgcgcttgc aaatgcaaaa gtgatacccg
     5761 ccattcccgt ccgcgaggcg gacaatacgg ttacattgca attctatccc gcttggaaat
     5821 cctttccgag tgaagacgcg caagccgacg cgcaacgtat gaaccgcttt atcgaagaac
     5881 gcgtgcgcga acatccggaa caatatttct ggctgcacaa gcgtttcaaa acccgtccgg
     5941 aaggcagccc cgatttttac tgactacata aaattacaaa acaaatcagg cgtttcagat
     6001 caaaaacccc gattgttttt gggaatttga aacccgggtt gtacaaacgg gatttgccgg
     6061 acggttttaa cggttcagtt gtttgtaaaa acaatgcttt tttaaaattg acaaaaaacg
     6121 aaatcggttt taaaggctta ttccgagaac aaaggggagt ggatgccgaa aacccggtta
     6181 atatattata gtggattaac aaaaaccaat acggcgttgc ttcgccttag ctcaaagaga
     6241 acgattccct aaggtgctga agcaccaagc gaatcggttc cgtactattt gtactgtctg
     6301 cggcttcgcc gccttgtcct gatttttgtt aatccactat aaaattaaat ttgtttaaaa
     6361 acataaagtt gtaaacaagt atctcatata agcctttttc attaaacaga tagtcagata
     6421 ttttgtgcta aaaatttata taatatttaa attaatatca agttataaaa aatatatgga
     6481 attttatttt gtttatttat aattttaagc aataatctaa atcaggcatt tttattcccc
     6541 tgttttaaaa aatatttggc aaggtgtgaa aaaaggcgta cattccgcta cacagaatta
     6601 cagatacagc ggagcaatgc cgtctgaaag gattttccgg tcagtcttgc ggttggtcgg
     6661 ggtttcatcg gatacggtga aacgaaagtt tgccggcgca gggttgagct acgcgggtaa
     6721 agccgcaggc gaaagcctgt attgtttgtg aagcgtaaat ctctgatttg aggtattggg
     6781 gcaatcctgt gggggttgcc tcttttttta tccgcctttt aatgacacaa taggcgcaag
     6841 tctgtttttg aatgcaaggt actgccatga atacgatttt ggctttcgat attgaaaccg
     6901 tacccgatgt gcagggtatc cgtacgttgt atgacttgcc gtcttccctg ccggatgacg
     6961 aagtggtgct gttcgcgcag cagaaacgcc gcgcacagac cggcggcgat tttatgcagc
     7021 accacctcca tcaggttgtg gcggtttcct gctgtatgcg ctgggggcag gacaaggttc
     7081 atgtcggcac catcggcgag atggacgatg gcgaagaagt cgtgattgcc aagtttttcg
     7141 aattggttga aaaacatacg ccgcaactgg tcagctggaa cggcggcggt ttcgacctgc
     7201 ccgtactgca ttaccgctcc ctgatatacg gcatcaacgc cgcgcgctat tgggatatgg
     7261 gcgacggcga tttcggcgac agccgcgatt tcaagtggaa caactacatc agccgttatc
     7321 accaacgcca ctgcgatttg atggatttgc tcgcgcttta ccagccgagg gcgaatgtgc
     7381 cgctggacga tatggcgaaa ctgtgcggct ttccgggcaa gctgggtatg gacggcagca
     7441 aggtgtggga ggcgttccac gcgggcaggc tgaaggaaat ccgcaattat tgcgaaaccg
     7501 atgccgtgaa tacgtatttg atgtatctgc gtttctgtct ggtcagcggc agattcgacg
     7561 cggacgaata cgaaatggaa atcaagcgga tcagaaacta tctctccgcc caaacagaag
     7621 acaaaccgca ttgggcagaa tttgtccaag cgtggaaata gaaacctgct gtccgaaggc
     7681 ggtacgcttg tgccgccgtt tcggtttcag acggcgtatg gtggattaaa ttcaaaccgg
     7741 tacagccttg cctctccttg ccttactatc tgtactgtct gcggcttcgt cgccttgtcc
     7801 tgatttttgt taatccgctt taaaagctgt atccgtgccg tctgaagcct tttccagtca
     7861 aaccaaacaa tattgaaaga aaaaaatgag caaaaccgtc cgttatctga aagattacca
     7921 aacgcctgcc taccgcattc ttgaaaccga actgcatttc gacattgccg aaccgcaaac
     7981 cgtcgtgaag tcgcgtttga cggtcgagcc gcagagggcg ggcgagccgc tggtgttgga
     8041 cggttcggca aaactcttgt ccgtcaaaat caacggcgcg gcggcggatt atgtgttgga
     8101 aggcgagacg ctgacgattg cagacgtacc gtccgaacgc ttcaccgtcg aagtggaaac
     8161 cgaaatcctg ccggcggaaa acaaatcgct gatggggctg tatgcttccg gcggcaatct
     8221 gtttacccag tgcgagccgg agggcttccg caaaatcacg ttctacatcg accgtccgga
     8281 tgtgatgtcc aagttcacga ccaccatcgt cgcggacaaa aaacgctatc ccgttttgct
     8341 ttccaacggc aacaaaatcg acggcggcga gttttcagac ggccgccatt gggtgaaatg
     8401 ggaagacccg tttgccaaac cgagttatct gtttgctttg gtcgcgggcg atttggcggt
     8461 aacggaagac cgtttcacca ccatgagcgg cagaaacgtc aaaatcgagt tttacaccac
     8521 cgaagcggac aaacccaagg tcggctttgc cgtggaatcg ttgaaaaacg cgatgaagtg
     8581 ggacgaaacg cgcttcgggt tggaatatga cttggatatt ttcatggtcg tcgccgtagg
     8641 cgatttcaat atgggcgcga tggaaaacaa gggtttgaac atttttaaca ccaagttcgt
     8701 cctcgccgac agccgcaccg ccaccgatac cgatttcgaa ggcattgaat ccgtggtcgg
     8761 acacgaatat ttccacaact ggacgggcaa ccgcgtaacc tgccgcgact ggttccagct
     8821 ttcgctgaag gaagggctga ccgtgttccg cgaccaagag ttttccggcg accgcgccgg
     8881 ccgcgccgtg cgccgcatcg agaacatccg cctgctgcgc cagaaccagt tccccgaaga
     8941 cgcaggcccg accgcccatc cggtgcgccc cgtcagctat gaggagatga acaatttcta
     9001 caccatgacc gtttatgaaa aaggcgcgga agtggtgcgg atgtatcata ccctgctcgg
     9061 cgaagagggc ttccaaaaag gcatgaagct atatttccaa cgccacgacg gacaggcagt
     9121 gacctgcgac gatttccgcg cggcgatggc ggatgcgaac ggcatcaatc tcgaccagtt
     9181 cgccttgtgg tacagccagg cgggcacgcc cgttttggaa gccgaaggcc gtctgaaaaa
     9241 caatgttttc gagttaacca ttaaacaaac cgtgccgccc acgcccgata tggcggacaa
     9301 acagccgatg atgattcccg tcaaagtcgg gcttctgaac cgcaacggcg aagcggtggc
     9361 attcgattat cagggcaaac gcgcaaccga agccgtgttg ctgatgaccg aagccgaaca
     9421 ggccttcccg ctcgaaggtg taaccgaagc cgtcgttccc tcgctgctgc gcgggttcag
     9481 cgcgccggtg tatctgaact atccgtacag cgacgacgac ctgctgctcc tgctcgccca
     9541 cgacagcgac gctttcacgt gctgggaagc cgcccaaacg ctctaccgtc gcgccgtcgc
     9601 cgccaacctt gccgcgcttt cagacggcat cgggttgccg aaacacgaaa aactgcttgc
     9661 cgccgtcgaa aaagtcattt cagacgacct cttggacaac gccttcaaag ccctgctttt
     9721 gggcgtgccg tccgaagccg aactgtggga cggcacggaa aacatcgacc cgctgcgcta
     9781 ccatcaggcg cgcgaagcct tgttggatac gcttgccgtc cgcttcctgc cgaaatggca
     9841 cgaattggac cgtcaggcgg cgaagcagga aaaccaaagt tacgaataca gccccgaaac
     9901 cgccgactgg cgcacgctgc gcaacgtctg ccgcgccttc gtcctgcgcg ccgaccccgc
     9961 gcacatcgaa actgttgccg aaaaatacgg cgaaatggcg caaaacatga cccacgaatg
    10021 gggcatcctg tccgccgtca acggcaacga aagcgatacg cgcaactgcc tgctggcgca
    10081 gtttgccgac aagttttcag acgacgcgct ggtgatggac aaatatttcg cccttatcgg
    10141 ctcaagccgc cgcagcgaca ccctgcaaca ggttcaaacc gccttgcagc atccgaaatt
    10201 cagtctcgaa aaccccaaca aagcccgttc gctcatcggc agcttcagcc gcaacgtccc
    10261 gcattttcac gcacaagacg gcagcggcta ccgcttcatc gccgacaaag tcatcgaaat
    10321 cgaccgcttc aacccgcagg tcgccgcccg cctggtgcag gcgttcaacc tctgcaacaa
    10381 gctcgagccg caccgcaaaa acttggtgaa acaagaattg cagtgcattc gggcgcagga
    10441 aggattgtcg aaagacgtgg gcgaaatcgt cggcaagatt ttgggttgag gcagtcaaac
    10501 agaaaaaaca agggtctgta ccagattagc agatatgtt
//
LOCUS       DAXDXW010000076        10316 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXDXW010000076 DAXDXW010000000
VERSION     DAXDXW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10316
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.077"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(499..1083)
                     /gene="rnhB"
                     /locus_tag="ACFB64_001757"
     CDS             complement(499..1083)
                     /gene="rnhB"
                     /locus_tag="ACFB64_001757"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC1210068.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKEQAVAWHVAASTPEEIASLNILHATMLAMKRAVYGLAARPEKIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRRPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(1151..3046)
                     /gene="mnmG"
                     /locus_tag="ACFB64_001758"
     CDS             complement(1151..3046)
                     /gene="mnmG"
                     /locus_tag="ACFB64_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC1210069.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRSQA
                     DRILYKASIREMLENQENLDLFQQAVEDVTLEGERISGVITAMGAEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGLNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSERLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGRVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(3219..4646)
                     /locus_tag="ACFB64_001759"
     CDS             complement(3219..4646)
                     /locus_tag="ACFB64_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC1210070.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDCDNHHFRGGPAYYITHGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            4805..5797
                     /gene="pdxA"
                     /locus_tag="ACFB64_001760"
     CDS             4805..5797
                     /gene="pdxA"
                     /locus_tag="ACFB64_001760"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC1210071.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAHGEGGLEVLHIPAAEAVEAGWLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLHKGIINAARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            complement(6027..8786)
                     /locus_tag="ACFB64_001761"
     CDS             complement(6027..8786)
                     /locus_tag="ACFB64_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFC1210072.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFLGYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNAGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHAACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     VPAAETSEKRSTANRSGSRANNRRRNPRRSKREGSKIEVREAAGKTAGQKARADKAET
                     RNNGNRRRNERGDRATERANEAEIQSRNVQPAAPVADAAPPETEGQTGKRRRNGSRNE
                     RGQTAPETAAVAETAVQTAENTPPEPYTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPLI
                     ATPVQTASSAVSNGDALIYDAAEKIRRAAADILPEGAAPKAAAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEAATVPAEEMIQV
                     ETRQG"
     gene            9279..10271
                     /locus_tag="ACFB64_001762"
     CDS             9279..10271
                     /locus_tag="ACFB64_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC1210073.1"
                     /translation="MKTHEISKGSVSLIGVAEHEAGQRLDNYLIKILKGVPKGYIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFEVVYEDDA
                     LLVVNKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAEPPPDLAQFAVMLENGTKM"
BASE COUNT         1966 a         2710 c         3206 g         2434 t
ORIGIN      
        1 cgtcattccc gcaaaaacaa aaaaatcaaa aacagaaaac tgaaatatcg tcattcccgc
       61 gcaggcggga atctaggtct gtcggtacgg aaacttatcg ggaaaaacgg tttttccaac
      121 cctgagactc cggattcctg ttttcgcggg aatccggttt tttgagtttc agtcattttt
      181 gataaattct tgcagctttg agtttctaga ttcccgcttt tgcgggaatg gcggtttgga
      241 agttacctga aattcaaaaa aaaaacggaa accggacgga ttggattccc gcctgcgcgg
      301 gaatgacgga ttttaggttt ttttgatttt ctgtttttcg cgggaatgac ggttgggaag
      361 tttcccgaaa cttaaaaaca actgaaaccc aaaaaactaa attaccgcct gcgggggaat
      421 gacggttcgg gttctttctc tttgaagttg cgatgctgga aatgccgtct gaagttcaga
      481 cggcattttt gtgccggttt aaaacaaggc ctgctgcgcg agcaggtttc tgacgggggc
      541 gaagtcgcgg cggtgttcgg gcagcacgcc gtattgtttg agggcttcca gatgctgctt
      601 tgtgccgtaa cctttgtgtt tgtcaaaacc gtattgggga cggcgttgcg ccagtgcgta
      661 catttccgca tcgcgtgcgg tctttgccaa aacggatgcg gcggagattt cgatgatttt
      721 gctgtcgcct ttgacgactg cttcggcagg gatgcctaaa tgttcgggaa tgcggttgcc
      781 gtcgatgaat attttttcgg gacgcgcagc caaaccgtaa acggcacgtt tcatggcgag
      841 catggtggcg tgcaggatgt tgaggcttgc gatttcttcg ggcgtggagg cggcgacgtg
      901 ccacgcgacc gcctgttctt taatcatttc ggcaagcgcg tcgcgttttt tctcgctgag
      961 ttttttggag tcggtcagtc cgggcaggtc gaatgtttcc ggaaggatga cggcggcggc
     1021 aaacacgctg ccgaccaaag gtccgcgtcc tgcctcgtcc acgccggcgg tcagtatgtg
     1081 catgatgttt cctgtcggga tggtacgatg ccgtctgaaa atggtttcag acggcatcgt
     1141 accgatgtgt ttatttcgcg tctttaaacc cgcgcttcaa atgcaccatc agcagtgcga
     1201 cggcggcagg cgtaacgccg gaaatgcggc ttgcctgccc gacggtttcg ggtttgtgct
     1261 ggttgagctt ttgttgcact tcggccgaca agcctttgac tctgccgtaa tcgatgccgt
     1321 cgggcagctt taaggtttcg atgtcgcggc ggctgtcgat ttcttcgttt tggcggtcga
     1381 tatagccttg gtatttgact tggatttcga cttgctcgat gacttcggca gagaggcgtt
     1441 cagacggcat cgcgccttcg agcgtcatca gtgcggcgta gtcgaggttc gggcggcgca
     1501 ggaggtcgtg caggttggct tcgcggctga gcttttgccc gaatacgcgg atttgttcgt
     1561 cttcggcgag tttttgcggc gtgtaccacg ttgttttcaa acgttggatt tcgcgttcga
     1621 cggcttcgcg tttttcgttg aacatgcgcc attgcgcttc acccaccaag ccgattttgt
     1681 agccgtcttc ggtcaggcgc atatcggcgt tgtcttccct gagttgcagg cggtattcgg
     1741 cgcggctggt aaacatccgg taaggttcgt tcaggccttt ggtgatgagg tcgtccacca
     1801 atacgccgag gtaggcttgt tcgcggcgca gcaggagcgg atcttgtccg cggacgtatt
     1861 gcacggcgtt cgcgcccgcc agcaatcctt gcgcggcggc ttcttcatag cctgtcgtgc
     1921 cgttgatttg cccggcgaaa aacagtccct caatggtttt ggtttcgagg cttgctttga
     1981 ggttgcgcgg atcgaagtag tcgtattcga tggcgtagcc ggggcgcagg atatgggcgt
     2041 tttcaagacc cttcatactg cgcacgagcg cgatttggat gtcgaacggc aggctggtgg
     2101 agataccgtt ggggtagtat tcgtgcgtgg tcagaccttc gggttcgagg aaaatctggt
     2161 ggctgtcttt gtcggcgaag cggttgattt tgtcttcgat agacggacaa taacgcggac
     2221 ccacgccttc gattttgccg gtaaacatcg ggctgcggtc gaagcctgag cggatgatgt
     2281 cgtgggtttg cgtgttggta tgcgtaatcc agcaggacac ttggcgcggg tgcatttcgg
     2341 cgttgccgcg cacggacatg acgggaacgg gcgtgtcgcc gggctgttcg gtgagttggg
     2401 aaaagtcaat cgtgcgtccg tcaatacgcg gcggcgtgcc ggttttcaga cggccttgcg
     2461 gcagcttcaa ttcgcgcaaa cgtccgccga gcgatttggc ggctgggtcg ccggcgcggc
     2521 cgccttcgta gttttccaaa ccgatgtgga ttttgccgga caagaacgtg cctgcggtca
     2581 gcaccacggc gcgtgcttta aactccgcgc ccatcgcggt gatcacgccg ctgatgcgtt
     2641 cgccctcgag tgttacgtct tcgacggctt gttggaaaag gtcgaggttt tcttggtttt
     2701 ccaacatttc gcggatggag gctttataca ggatgcggtc tgcctgcgag cgcgtggctc
     2761 gtactgccgc gcctttgctg gcgttcaggc ggcggaattg gatacctgat ttgtcggttg
     2821 ccaacgccat tgcgccgccg agcgcgtcga gttcgcgtac caaatgccct ttgccgatgc
     2881 cgccgataga ggggttgcac gacatttgtc cgagcgtttc gatattgtgt gtgagcaaaa
     2941 gcgtctgcgc gcccatacgg gcggcggcaa gcgcggcttc cgtgccggcg tgtccgccgc
     3001 cgacgacgat gacatcgtag gttttggggt aaatcatgtg ggtcatgtgt gtattgccta
     3061 tcggtttttc agacggcatt ccaagcagga tgccgtctga aaaacgaaac ggattcaaaa
     3121 gtaaagggtt gggattgtac gcctgttcgc cctgttttta cagtgcgcgg aaagggaaaa
     3181 gccgcttcgc ggggaagcgg ctccggtaag ggtgggattt accaaacatc ggatttgatg
     3241 cggcgtttca ggcccggatg ttcggaaagt ttgaactcgg ggtctttgcc cattttcagc
     3301 ttggcggtgt aatcgcgcag cagcataaac gccaatggcg agagcagcag gatggcgacg
     3361 aggttgatcc acgccatgat gcccatcgcc atatccgcca tatcccagac caaaggcaca
     3421 ttggcaaccg cgccgaaata gacccacgcc aaaaccagca tacggaaaac ggcggtaatc
     3481 agccaatggc ttttgatgaa ttggacgttg gactcggcat aggcatagtt gccgataacg
     3541 gtggaaaagg caaacataaa caggatgacg gcgaggaaac ccgcgcccca ttgccccact
     3601 tggctgacaa tcgccgcctg cgtcagcgcc gcaccgctca aatcgccata aggctgttgg
     3661 taaatcaaga tgatgaaggc ggtgcaagaa caaacgatga tggtatcgac aaacacgccc
     3721 agcatttgaa tcataccttg cgaaacaggg tgtttcactt cggcggcggc ggcggcgttc
     3781 ggcgcggaac ccatacccgc ctcgttggaa tacaggccgc gtttgatgcc catcatcatc
     3841 gtttgcgaaa tcagaccgcc gagtaagccg cctgctgccg cgtcgaattt gaacgcgccc
     3901 gaaaaaatct gaccgaacac gtccggaatc atcggaatat tggtcaaaat gatgaaaagc
     3961 gcgataaaga ggtacaaaac cgccatcagg ggcacgacga tttccgccgc tttggatatg
     4021 cgcctgatgc cgccgaagat aatcggcgcg gttaaaatca ccagggcgac gccgacataa
     4081 tgaggctccc agccccacgc cgctttgacg gtatcggcga tggtattggt ctgaaccgct
     4141 tcaaacacaa aaccgaaaca gaaaatcagg ctcagggcga acaacacgcc cagccatttc
     4201 tgccccagac cgtgcgtgat gtagtaggca gggccgcccc ggaaatggtg gttgtcgcag
     4261 tcgcggactt taaagagctg cgccagcgaa gattcgacaa acgccgaact catgccgatt
     4321 aaggcggtta cccacatcca aaacaccgcg cccggcccgc cgactttgat ggcgattgcc
     4381 acgcccgcga tattgcccac gcccacgcgg ctggcaaggc cggttacaaa tgcctgaaac
     4441 ggcgtgatgc cgtgagggtc gtccccctgt ttgcggccgc cgagcatttc tttgatgctg
     4501 cgcccgaaca ggcggaattg gacaaagccc gtggttacgg tgaagaaaag ccccgtaccc
     4561 aaaagcatat aaaccaagta tgaccacatc ggatcgttga tggcgccgac ccagccgtgc
     4621 agccattcgg taaagttctc gttcatatcg cttccttaaa gttgaaaccc gcacatatgg
     4681 cggtatgcaa gcagggttta aattttgtaa acgcccattc tagcagattg tcaacaaaat
     4741 cagaaaaatt tacatcgccg cgcgcctgtg gcgttagaat cgcgttttgt ttggagcaaa
     4801 cacgatgaaa cagcctgttt ttgccgttac ttccggcgag cctgccggca tcggccccga
     4861 tatttgtttg gacttggcgt ttgcacgcct gccctgccgc tgcgcggtat tgggcgacaa
     4921 acacctgttg cgcgcgcgcg ccgaagcctt gggcaaaagc gtcgtcctgc gcgacttcga
     4981 tccggaatca ggcggcgcgc acggagaagg cgggctggaa gtgctgcaca tccctgccgc
     5041 cgaagcggtt gaggcagggt ggctcaatcc cgccaacgcc gcctatgtgc tgcaactttt
     5101 ggacaccgcg ctcgcaggca tttcagacgg cattttcgac ggcatcgtta ccgcgccgct
     5161 gcacaaaggc atcatcaacg ccgcgcgcgc aagcacaggt tttttcagcg gacacaccga
     5221 atatctggcg gaaaaaagcg gcacggggca ggtcgtgatg atgcttgccg gcaaaggcct
     5281 gcgcgtcgcc ctcgtaacga cccacctgcc gctgaaagac gttgccgccg ccatcacgca
     5341 accgctgatt gaaagcgtcg cacgcatttt gcatcacgac ttaaaacaca aattcggcat
     5401 caaaaatccc aaaatcctag tcgccgggct caatccccac gccggcgaag gcggacacct
     5461 cggacacgaa gaaaccgaca ccattatccc cgcgctcgaa aacctgcgcc gcgaagggat
     5521 aaacctcgcc ggcccgtatc cggcggacac attgttccaa ccgtttatgc tcgaaggtgc
     5581 ggatgccgta ttggcgatgt accacgacca agggctgccc gtgttgaaat accacagctt
     5641 cgggcagggc gtgaacatca cgctggggct gccctttatc cgcacctccg tcgatcacgg
     5701 caccgcgctc gatttggcgg caaccggcag ggcggactcc ggcagcctga taactgccgt
     5761 ggagaccgcc gtcgagatgg cgcgcggcag cctttaaaga tgataaaaga cccgtcattc
     5821 ccgcgcaggc gggaatccgg accttcgggc ggcggcaata ttcaaaggtt atccgaaagt
     5881 ttgaggttct agattcccgt tttcacggga atgacgaaag gtggcgggaa tcacgggaat
     5941 ggcgaagttt caggcggtat tgcaggtatc cgaaaccatg caaaaagagg ttctgcggaa
     6001 cagaacctct ttttgccgcc gtcggttcag ccttgccggg tttcgacttg gatcatttct
     6061 tcggcaggga cggttgcggc ttcagacggt tttggctgtt cggaacggcg caaaccgcgt
     6121 ccggcttgga cttcgggttg tgccgcccat gccttcaatg cggcagggtc ggtttcgatc
     6181 aggacgagtc cgccggtttg tgcggtttcc cgtgcctgtt ccgccgcagc cgtaaaggtt
     6241 gcggtttcag acggcatttc ctgtgctgcg gctttcggtg ccgcgccttc gggcaggatg
     6301 tcggcggcgg cacggcggat tttttccgcc gcatcataaa tcagtgcgtc gccgtttgaa
     6361 acggcggaag atgccgtctg aacgggtgtg gcgatgagcg gatcggcaat gctgacggta
     6421 atcggcgcgt ttgcgtcggt ttcgccgaaa acgtgcgcgg cggcggaacg gactttgtcg
     6481 gcggtgtcgt gaatattcag gtactgctcg attttggcgg cagacgggat attgcgtttt
     6541 ttgccgtttt gacggcggtc gcgctgattg ttgcgttcgc ggcgttcttt ggcatcgcgg
     6601 ctgtcgcgtt cgcggcggtt gcgttcggat ttgggcttgc tgcctttgtc ttctgcggta
     6661 tacggttcgg gcggcgtgtt ttccgctgtc tgaacggctg tttcggcaac ggcggcggtt
     6721 tccggtgcgg tttggccacg ttcgttgcgg ctgccgttgc ggcggcgttt tccggtttga
     6781 ccttcggttt cgggcggtgc ggcatctgca acgggtgcgg caggctgtac gttgcggctt
     6841 tggatttccg cttcgttggc gcgttcggtg gcacggtcgc cgcgttcgtt gcggcggcgg
     6901 ttgccgttgt tgcgcgtttc ggctttgtcg gcgcgcgctt tctgtccggc agttttgccc
     6961 gccgcttcgc ggacttctat tttgctgcct tcgcgtttgc tgcggcgcgg gtttcggcgg
     7021 cggttgttgg cgcggctgcc gctgcggttt gccgtgctgc gtttttcgga ggtttcggca
     7081 gcgggtacgg cttgggtttc gctgccgccg aaaatgcgtt tgagccatgc tttgaagctg
     7141 tcccaccagg aggttttttt ctcgggggcg gcagtcgggg cggggctggt gtggcgcacg
     7201 cctttgacgg cgggttcggg gcgggcggct ttggcttttt cgccgccgaa aggtttggcg
     7261 gattcgtctt cttcaggctc ggcaacgcgc ttgtagctcg gttcgccgtc ttcttctacg
     7321 tcgtcggtgc ggatgcggtt gatttcgtag tgcggatttt cgaggtggat gttcggaatc
     7381 aggacgacgt tcacatccaa acgctcttcc attgcaaaca gctcggcgcg tttttcgttc
     7441 agcaggaagg tggcgacatc gacgggcact tgggcgcgca cttcgccggt gttgtccttc
     7501 atcgcttctt cctgaaccat gcgcaaaacg tgcagggcgg tggattcgat gccgcggatc
     7561 acgccggtgc cggcgcagcg cggacaggcg gcgtggctgc tttcgcccaa agcgggtttc
     7621 aggcgttggc ggctcaattc caaaagtccg aaacgggaga gtttgcccat ctgtacgcgg
     7681 gcgcggtctt ttttgagtgc gtcgcgcagg acgttttcca catcgcgctg gtgtttgggg
     7741 ttttccatgt cgatgaagtc gatgacgacc aagccgccca agtcgcgcag gcgcatttgt
     7801 cgggcgactt cttcggcagc ttccatattg gttttgaagg cggtgtcttc gatgtccgcg
     7861 ccgcgtgtgg cgcgtgcgga gttcacgtcg atggagacga gggcttcggt gtggtcgatg
     7921 acgatcgcgc cgccggaggg caggctgacg ctgcgcgaga acgcgctttc gatctggtgt
     7981 tcgatttgga agcgggaaaa cagcggcgtg tggtcttcgt agagtttcag acgacctgcg
     8041 ttgcccggca tgacgtagct catgaactcg gcaacttggt cgtaaacttc ttgattgtcc
     8101 accaaaattt cgccgatgtc gggacggaag tagtcgcgga tggcgcggat gagcaacgag
     8161 ctttccataa agagcaggta ggggtcgtga tgcgcttttc ctgcttcttc gattgcctgc
     8221 cagagttgtt tgaggtagtt caaatcccat tccaactctt ccgcgctgcg gccgatgccg
     8281 gcggtacgcg cgatgatgct catgccgttc ggaatgtcga gttgcgccat cgcggctttg
     8341 agttcttgac gctcttcgcc ttcgatacgg cgggatacgc cgccgccgcg cggattgttc
     8401 ggcatcaata ccagatagcg tccggcgagg ctgatgaagg tggtcagcgc cgcgcctttg
     8461 ttgccgcgct cgtctttttc gacttggacg atgacttcca tgccttcttt gagcacgtcc
     8521 tgaatgcgcg cgcgcccgcc ttcgtagccg aggaaatacg aacgcgatac ttctttaaac
     8581 ggcaaaaagc cgtggcggtc ggttccgtaa tcgacgaaac acgcttccag cgacggctcg
     8641 atgcgggtaa tgatgccttt gtagatattg cctttgcgct gttctttgcc cagcgtttcg
     8701 atgtccaaat ccagcaggtt ttgtccgtcg acgatggcaa cgcgcagctc ttcggcctgc
     8761 gttgcgttga ataacattct tttcatgatc acctcgtgga caggcggcat tcgggcgcgc
     8821 cggtccggtt cggcattccg taaggctggg tttccgatgt cttcggataa aaccggtaat
     8881 cagtttttga gttgaaaatc cgcagggatg cacgttccgg ggaaccgtgt gcggaagggt
     8941 cggataaaga aggctataaa gatcgatgcg gcggtctgcc tgccgcgttc cgaacgctgc
     9001 ggtcggaaaa atgggcgccg gcttcttctt gttatcgtga tgcctgtgtt ttcgggcggt
     9061 ttgcgttcgg ggcttggggc tccgctgccg tcttacttcc gcgccgaaac ggcaaaatca
     9121 attcaaactt gactacgttc tgcgcctgcc ggctgggaac aggcgcaggg aaaatgcttt
     9181 gcggagtgcg tttttaatat aaaattccgt tttaaagtaa accgtttcag ggggcgcggc
     9241 ggacgcgctt tctgctgaaa cggatgttcg gattatagat gaaaacgcac gaaataagca
     9301 aaggttcggt cagcctgata ggggttgccg aacatgaggc gggtcaacgc cttgataact
     9361 atctgataaa aatcctcaag ggcgtgccca agggctatat ccaccgcatt atccgcgccg
     9421 gcgaggtgcg gttgaacaag aaacgctgca aacccgacag ccgtattgcg gagggagata
     9481 cggtgcggat tccgcctgtg cgcgtggcgg agaaggaaat gccgtctgaa aggcgtgccg
     9541 ccgtaccggc gcgtgcgttt gaggtggtgt atgaggacga cgcgctactg gtggtcaata
     9601 agccttccgg tgtggcggta cacggtggca gcggcgtgag tttcggcgtt atcgaacagt
     9661 tgcgccgcgc ccgtccggag gcgaagtatt tggagttggt ccaccgtttg gacaaggata
     9721 cgagcggctt gttgatggtg gcgaagaaac gcagcgcgct cgtcaaactg cacgaagcta
     9781 tccgtaacga ccaccccaaa aaaatctacc ttgcgctggg ggtgggcaaa ctgccggacg
     9841 acaatttcca tgtcaaactg cccctgttca aatataccgg cgcacaaggc gaaaagatgg
     9901 tacgcgtcag cgaggacggg cagtcggcgc atacggtgtt ccgtgtgtta agccgtttct
     9961 cagacggcat tttgcacggt gtcgggctgt cgcacctgac tttggtgcgg gcaacgctga
    10021 aaacgggccg tacgcaccaa atccgcgtgc atctgcaatc tcaaggctgt ccgattgcgg
    10081 gcgacgaacg ctacggcgat tatcaggcga accggcgttt gcagaaattg ggtttgaagc
    10141 gcatgttttt gcacgcgtcc gagctgcacc tgaaccatcc gctcacgggt gagccgctgg
    10201 tgttgaaggc ggagccgccg ccggatttgg cgcagtttgc ggtgatgttg gaaaacggga
    10261 cgaaaatgtg aaccccgatg ccgtctgaag gcttcagacg gcatcggggc gtgaaa
//
LOCUS       DAXDXW010000077        10194 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXDXW010000077 DAXDXW010000000
VERSION     DAXDXW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.078"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..129)
                     /locus_tag="ACFB64_001763"
     CDS             complement(<1..129)
                     /locus_tag="ACFB64_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HFC1210074.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAE"
     gene            374..1282
                     /gene="hslO"
                     /locus_tag="ACFB64_001764"
     CDS             374..1282
                     /gene="hslO"
                     /locus_tag="ACFB64_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFC1210075.1"
                     /translation="MNQTAINRADARTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRCALGELLAAGVLLSGNLKNEGTLIVQVQGQGKLKMLVAEATSDRTVRATARWDET
                     AEIADDESLGDLLGGNGVFVLTLQPKDGEPWQGVVPLEGGSIAQMLVNYMKRSEQLDT
                     HIALSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAELDAQHVLYRLFH
                     ETPPRVFEPETFESSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            complement(1368..2822)
                     /gene="mgtE"
                     /locus_tag="ACFB64_001765"
     CDS             complement(1368..2822)
                     /gene="mgtE"
                     /locus_tag="ACFB64_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC1210076.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLAELHPADVADVLESLPPRERNIVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITIDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLSLATLFLL"
     gene            complement(3056..3808)
                     /gene="gloB"
                     /locus_tag="ACFB64_001766"
     CDS             complement(3056..3808)
                     /gene="gloB"
                     /locus_tag="ACFB64_001766"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC1210077.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTRFTFGNGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTVEQLYDSFQRFNQLPEGT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAERTPTLPVTLAHERRVNPFLRTEIP
                     AVRQRAEALVGKTLNSGLEVFAALRELKNAYR"
     gene            4040..4351
                     /locus_tag="ACFB64_001767"
     CDS             4040..4351
                     /locus_tag="ACFB64_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFC1210078.1"
                     /translation="MPQQTMNLMRECIPIFTVLSDENRHQILHVLWKHGRMNVNELTE
                     HLHLSRPAVSHHLKIMLQAGAVAVEQVGKERFYSIAMADAVARLKQLADLMAQNCPLS
                     K"
     gene            4425..5636
                     /locus_tag="ACFB64_001768"
     CDS             4425..5636
                     /locus_tag="ACFB64_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HFC1210079.1"
                     /translation="MLFTPFTFQNGKTAKNRIFKSAMEEQLAQNDQPSEKLVRLYGAW
                     AEGGAGVLVTGNVMVAESGKGSINDVLISDDRALEMLKKWAKARMQNDTLLIMQINHA
                     GKQSPAVVNKTPLAPSAVPLVGMNGFINPPRELSADEINGLIQQFVQTAKIAEQAGFS
                     GVQIYAVHGYLISQFLSPHHNRRQDQWGGSLENRMRFLLETYTAIRAAAGKDFLVGVK
                     LNSADFQKGGFDESESVQVVQKLSEMGIDFIEVSGGNYESPQMLAAKDSTRKREAFFI
                     DYAEKARAASQAPLIITGGFRSQTAMEDALSSGHLDLVGIARPFALVPDLANKMQNRT
                     YQTVQADRIQTGVAFVDKKAGAMLEMNWYMTQMDLIGQGKQSNPKLSAWKVLLKTLRE
                     NGKAGLSTGRA"
     gene            complement(5707..9663)
                     /gene="purL"
                     /locus_tag="ACFB64_001769"
     CDS             complement(5707..9663)
                     /gene="purL"
                     /locus_tag="ACFB64_001769"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC1210080.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPEAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLKSET
                     FSTVDVLGGGKEALVKANTETGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIKGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDQNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVKDVRKTVTPELKNVEGSVLLFIDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDAGRLKAFYSVIQQLVAEDKLLAYHDRSDGGLFATLAEMAFAARCGISADIDCLMD
                     KFLPIHLPDFQGDPAEDLSDELYNHAAIKILFNEELGAVIQIRQKDRDYVDAAFETAG
                     LTDAVSRIGSPDFDNEFISFFGYGYFLEQNRADLQRAWQETSHAIQRLRDNPACADSE
                     FALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFTRA
                     GFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQFA
                     AFFADPNTLTLGVCNGCQMVSNLAEIIPGAETWPKFKRNLSEQFEARLNMVHVPKSAS
                     LILNEMQDSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNPNG
                     SPQGIAGITNADGRVTIMMPHPERVYRAAQMSRKPEGWTELSGWYRLFAGARKALG"
     gene            9814..10152
                     /locus_tag="ACFB64_001770"
     CDS             9814..10152
                     /locus_tag="ACFB64_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC1210081.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
BASE COUNT         2258 a         2649 c         2965 g         2322 t
ORIGIN      
        1 ttcggcaaac tgtctgagaa tctgctcgcg gctgctgagc aggttggtca actcgtcggc
       61 aagggcgggg cggacggcaa acatcagcca caaaaccgcc caaactgccg gtttgaaaaa
      121 agaatccata tcggtgtttc cgcgcaggag gcatcgtgcc gccgtgcgat gttatgctgt
      181 atcagtcgaa aatatcttgt tgattgtata taaaaaaacg gctgtttggc aaaaaacggc
      241 ttgttagatt gagttaatat gttgattttt atataattta ttgagaattt gggaagcgca
      301 tccgccgtcc gccttgtttt cgcgccgacc gcaaccatat agccgccatc cgaacaatca
      361 gtcagagaaa atcatgaatc aaaccgccat caaccgcgcc gatgcgcgta cccgctttat
      421 cttcgacgat atgcccgtgc gcgggctgca cgtccgtctg gaaaacgtgt ggcagcacat
      481 tgtaaaacag aaaaactatc ccgccgccat ccgctgcgcc ttgggagagc tactggctgc
      541 cggcgttctg ctgtcgggca accttaaaaa cgaaggcacg ctgattgtgc aggttcaggg
      601 gcagggaaaa ctgaaaatgc tggttgcgga agcgacttcc gaccgtaccg tccgtgcaac
      661 cgcgcggtgg gacgaaaccg cagaaatagc cgatgacgaa agcctcggcg accttttggg
      721 cggcaacggc gtgttcgtat tgacgctgca acccaaagac ggcgaaccct ggcagggcgt
      781 agtgcctttg gaaggcggca gcatcgcgca aatgttggtg aactatatga aacgttccga
      841 acagcttgat acgcacatcg ccctgtctgc aagcgatgaa gcggcgggcg ggctgctggt
      901 gcagcgtctg cctgaagagg tgttggatga agaggcatgg gaacacgtca gtacgctggc
      961 gcgcacgctg acggcggagg agctggcaga attggacgcg caacacgttt tataccgcct
     1021 gttccacgaa acgccgccgc gcgtgttcga gccggaaacg tttgaatcct catgtacctg
     1081 ttctcggggc aaggtcagcg atatgctgtt gatgctgggc ggggaagaag tcggcggcgt
     1141 ggtggcggaa caaggcagca tcgaagtcga ttgcgacttc tgccacagca aatacgtgtt
     1201 tgatgaaacc gatgtcaacg cgctgttcgg ggaggatgtg gtcggcgttg ccaaagggct
     1261 gccccgacat accgtccaat aatcttgagc aacaaggatg aatggtcgaa tgccgtctga
     1321 agcagcttct gcttcagacg gcatttttgc gcgggtcggg acggcattta aagcaggaac
     1381 agcgtggcga gactcaagaa aatcaggaag ccgccggagt cggtaacggc ggtaatcagc
     1441 accgagctgc ccagcgcggg atcgcgtccg aatttttcca ttaccacggg aatcaatacg
     1501 ccgaccgttg ccgccagcag gaggttgagc gtcatcgcgg caaccatgac caaaccgatg
     1561 ccgaggctgc cgtaaagcag ccacgaaacc gctcccatca ccgtccccca aataatgccg
     1621 ttgaccaagg cgacgccgac ttcttttttc aacagccttc ccgcctgcat atccgtcagc
     1681 tgccccatcg ccatcgcgcg gacaatcatg gtaatcgtct ggttgcccga gttaccgccg
     1741 atgccggcga cgatgggcat cagcgcggcg agtgcgacga ttttttcgat actgccttca
     1801 aacgcgccga tgacgcggct ggcgaggaag gcggtgcaga ggttgacggc gagccacatc
     1861 cagcggtttt tgacggaatc caagacgggg gcgaacaggt cttcctcttc ctgcaaacct
     1921 gccatgttca acatatccgc ttcggattct tcgcggatca cgtccaccat ctcgtcgatg
     1981 gtaatcctgc cgatgagctt tttgttttca tcaacgacgg gcgcggtaac caagtcgtag
     2041 cgttcaaacg cctgcgccgc ttcttccacg tcgtcctcgg cgcggaaacg cacgacatct
     2101 ttcgccatca cgttttccac caaatcttcg ggatcggcga ccaaaagttt gcggatgggc
     2161 agcacgccct gcagcacgtc gttttcatcg acgacaaaaa tcttgtcggt atggtcgggc
     2221 aggctgtcga agcggcgcag ataacgcagc accacttcgc aggcgacatc ggcgcggatg
     2281 ctgaccaact cgaagtccat aatcgcaccg acttggttgt cttcatacga cattgccgcc
     2341 ttgacttggg cgcgctcttc ctcatcgcgc gtctgtaagg cttcataaac cacttggtgc
     2401 ggcaaatcgt ctgccagctc cgccaattcg tccgcatcca aatcatcgac cgccgccaac
     2461 aattcgtcct tgtccattga ctcgatcagc gtttctcgca ccgcgtcgga tacttccagc
     2521 aatacttcgc cgtcgtcttc gggtttgacc agaagccaaa cgatattgcg ttcgcgcggc
     2581 ggcagggact ccaagacgtc ggccacgtcg gcagggtgca actcggccaa gaggacggtc
     2641 agctcggtca gtttgtcgcg cagcggcgca tcttcgagcg gtgtgccgtt ttcgatttgt
     2701 tcaaaagcag gttcgaggat ttcgcagagg gagtggacac ggtcgaaatc ggcggaaggg
     2761 cgttcggcat ggttttgacc ggtttcgccg tcgtgtgtcg gaggagtgtg ttcgatgctc
     2821 ataaatgctc cgcccgccgt gtgcggggag gcattccggc gggatggtta ttgtcgtttg
     2881 gaatcggaag gggtgttcag taaaaacgaa ctgggaaggt ccataataaa agcccgaaaa
     2941 atcaggcacg ggattggaaa cagcactatt ctactccctt ttgaaacggt ttactatttt
     3001 taacgcaaat gccgtctgaa acgcgggttt cagacggcat ttttcggagg gcaggtcagc
     3061 ggtaggcgtt tttcagttcg cgcagggcgg cgaatacttc caaaccgctg ttcagcgttt
     3121 tgccgaccag ggcttcggca cgttggcgga cggcggggat ttctgtccgt aagaacgggt
     3181 tgacgcggcg ttcgtgcgcg agggtaacgg gcagggtggg cgtgcgttcc gccgccttca
     3241 atgccgtctg aatgtcggcg ttgtccggct cgatatgggc ggcgaaacgc aggttggcgg
     3301 cggtgtattc gtgtgccgga tagaacaggg tgccttcggg taattggttg aaccgttgga
     3361 agctgtcgta aagctgttcg accgtgccgg taaacacgcg tccgcagccg gcggaaaaaa
     3421 gggtgtcgcc gcagaaaacg tgtatgccgt ctgaagtttc gagaaggtag ctggtgtggc
     3481 ggtctgtgtg gccgggtgtt gcccaaacgg taacctgacc gttgccgaag gtgaatcgtg
     3541 tgccggcggt tacggtgtgg gtggcggctt cgatgtcgga ttcgccgtaa acgggcgatt
     3601 ccatgtagcc gcgccagagt gccgccgcac cgccttcgtg gtcgggatgg gggtgcgtta
     3661 cccaggtttg ggcgagcatg agtcggttgc gtacgaggaa ttccaagacg ggcgagggtt
     3721 cggaaggatc gacgcagacg gcatggttgc cgtgctgtat catccagatg tagttgtcgg
     3781 tcagggcttt gacgggggta attttcatga gtgttccttg atggttcgga tgccgtctga
     3841 aaggtgtatc cgaaaggcgt taatatacag cggaacccta gcaaacgcca tcatgctgtt
     3901 tcaggcggca ttttttctta taaaaacctt tcagcaacaa tatattgcca aactgcaaac
     3961 caaactgcct ttgataactg ccgaccttcc ctctaaaata cgttcgaatt tttaaatata
     4021 taaactcaat agatcgaata tgccgcaaca aaccatgaat ttaatgcgcg agtgcatacc
     4081 catttttacc gtattgagcg atgaaaaccg ccatcagatc ctgcatgtat tgtggaaaca
     4141 cggtaggatg aatgtgaatg agctgaccga gcatctgcat ctgtcgcgcc ctgctgtgtc
     4201 gcaccattta aaaatcatgc tgcaagccgg agcggtggcg gtggagcagg tcggcaaaga
     4261 gcggttttac agtattgcca tggcggatgc ggtggcgaga ttgaaacagc ttgccgattt
     4321 gatggctcaa aattgcccgc tttcaaaata gggttatccg ttggcccttt atttttaccc
     4381 aaatacgttt acatttttaa acttatcaac ttaaaggaac gatcatgtta ttcacgccat
     4441 tcacttttca aaacggcaaa accgccaaaa accgtatctt caaatccgcc atggaagagc
     4501 aacttgccca aaacgaccag ccgtctgaaa aacttgtccg cctgtacggc gcttgggcag
     4561 aaggcggcgc aggcgttttg gtgacgggca atgtgatggt ggctgaaagc ggcaaaggtt
     4621 cgataaacga tgtgttgatt tcagacgacc gcgcccttga aatgctgaaa aaatgggcga
     4681 aagcccgcat gcaaaacgac acgctgctca tcatgcaaat caatcatgcg ggcaaacagt
     4741 cgcctgcggt ggtcaataaa acaccgcttg caccgagcgc cgtgccgctg gtgggcatga
     4801 atggctttat caatccgccg cgcgaattga gtgccgatga aatcaacggg ctgattcagc
     4861 aattcgtaca aactgccaaa attgccgaac aggcaggctt ttcaggcgta caaatttacg
     4921 ccgtacacgg ttatctcatc agccaattcc tctcgccgca ccacaaccgc cgccaagacc
     4981 aatggggcgg cagtttggaa aaccgtatgc gcttcctttt ggaaacctac accgctattc
     5041 gcgctgccgc gggcaaagat ttcttggtgg gcgtaaaact caattcggca gatttccaaa
     5101 aaggcggatt tgacgaaagc gaatcggtgc aggtggtgca aaaactgtcg gaaatgggca
     5161 ttgattttat tgaagtttcc ggtggcaact acgaaagccc gcaaatgctc gccgccaaag
     5221 acagcacccg caaacgcgaa gccttcttca tcgattacgc cgaaaaagcc cgtgcagcca
     5281 gccaagcccc gctgattatc accggcggat tccgttcgca aaccgccatg gaagatgcct
     5341 tatccagcgg tcatttggat ttggtcggca tagcccgccc gtttgcctta gtccctgatt
     5401 tggcgaacaa aatgcaaaac agaacttacc aaaccgtaca agcagaccgc atccaaactg
     5461 gcgtagcatt tgttgataaa aaagcgggtg caatgttaga aatgaactgg tatatgacgc
     5521 aaatggattt gatcggacaa ggaaaacagt ccaaccctaa attgtcggcg tggaaagtat
     5581 tgctgaaaac tttgcgggaa aacggtaaag cgggattgag taccggcagg gcttgagaat
     5641 ggtcaaaatg ccgtctgaaa cgggtttcaa aacctttttc agacggcatt tgtttgggca
     5701 gacggtttag cccaaggctt tacgtgcgcc ggcaaagagg cggtaccagc cggacagttc
     5761 cgtccagcct tccggtttcc ggctcatttg tgcggcacgg tacacgcgtt cggggtgggg
     5821 catcataatg gtaacgcggc cgtcggcgtt ggtaatgccg gcgatgccct gcggcgagcc
     5881 gttggggttg agcggatagg tttgggtaac ctggttttga ccgtccacat attgcagcgc
     5941 gatgcccaaa tcggcggaaa tattgccgcc gtgaagcgcg aagtcggcgc ggccttcgcc
     6001 gtggctgacc acgacaggca ggctggagtc ttgcatttcg ttcaaaatca gcgacgctga
     6061 tttggggacg tgaaccatat tcaggcgcgc ttcaaactgc tcgctcaggt tgcgtttgaa
     6121 cttcggccat gtttctgcgc ccgggataat ttcggcaagg ttgctgacca tttggcagcc
     6181 gttgcacacg cccaatgtca gcgtgttcgg atcggcaaag aaggcggcga attggtcgcg
     6241 caaggcaggg tggaacagga tggatttcgc ccagccttca cccgcgccga gtacgtcgcc
     6301 gtagctgaag ccgccgcacg ccgccagcat tttgaagtcg gcaaggcgga agcggccagc
     6361 catcaggtcg gacatatgca cgtcgtaggc atcgaatccg gcgcgggtga aggcggcggc
     6421 catttcgatt tgcccgttta cgccctgttc gcgcaggatg gcgattttag gtttcgcgcc
     6481 actgttgata aacggcgcgg cgatgtcttc gttcacgtcg aacttcacgt cggcaaacaa
     6541 tgcgctgcgt tcgttgtcgc caatcagggc gaactcgctg tcggcgcagg cagggttgtc
     6601 gcgcaggcgt tggatggcgt ggctggtttc ctgccacgcg cgttgcaggt cggcgcggtt
     6661 ttgttccaag aaataaccgt aaccaaagaa agaaataaac tcattgtcaa aatcaggaga
     6721 gccaatccgg ctgaccgcat cggttaagcc ggccgtttca aatgccgcat caacataatc
     6781 cctatctttt tggcggattt ggataaccgc gcctaattct tcattgaata agattttaat
     6841 ggcggcatga ttataaagtt cgtcagataa gtcttcggca gggtcgcctt gaaaatccgg
     6901 gagatgaatc ggcaggaatt tatccatcag gcaatctata tcggcactaa taccgcaccg
     6961 tgccgcaaac gccatttccg ccagcgtcgc aaacaaaccg ccgtcgctgc ggtcgtgata
     7021 cgccaaaagt ttgtcttcgg cgacaagctg ttgaattacg ctgtaaaacg ctttcagacg
     7081 gcctgcatcg tccaaatcgg gcgcgtcgcc gctcatgttg ttgtacacct gaccgaacgc
     7141 cgagccgccc atgcgcgctt tgccgaagcc caaatcgata aacagcaata cgctgccttc
     7201 gacgtttttc aattcgggcg taacggtttt tcgcacgtct ttaacaggcg cgaacgcgga
     7261 aataatcagg ctcaacggcg aaaccacgga ttttttctct tcgccgtcct gccacacggt
     7321 tttcatcgac aggctgtctt tacctacggg aatgctcaaa tccaatgcct ggcaggcttt
     7381 agaaacggct tcgacggtgc ggtagagttt ttcgtcttcg ccttcgttgc cgcacgccgc
     7441 catccagttg gcggaaagtt tgatgttgcc gatgtcgccg atgttgactg ccgcgatgtt
     7501 ggtgatggct tcgccgacgc acattctgcc cgaggcgggc gcgtcaaaca gggcgacggc
     7561 cggtttttcg cccatagaca tcgcttcgcc gcgataggtg ttgaagccca tcatggtaac
     7621 ggcgcagtcg gctacggggg tttggtattt gccgaccatt tggtcgcggt gggtcatgcc
     7681 gccgacgctg cggtcgccga tggtaatcag gaagtttttg gcggctacgg caggcaggcg
     7741 caaaacgcgg taggcggctt cggtgatgtc gatatcgccc gcgtgaaacg gttttttgga
     7801 cggcgtaacc gttttgtcgg tacgcgtggt tttgggcggt ttgccgagca agacgttcag
     7861 cggcaaatcg acggggttgt tggagaacaa atcgtcgcgc actttcaaat gaccgtcgtc
     7921 ggtcgccgtg ccgactacgg caaacgggca gcgttcgcgt tcgcagatgg cgcggaaggt
     7981 gtccaaatct ttttccaaaa tcgacaagac ataacgctct tgcgattcgt tacaccagat
     8041 ttgcaaaggc gttaaaccgt gttcttccaa cggcacttcg cgcagctcga ataccgcgcc
     8101 gcgtccggca tcgttgacca attcagggaa agcgttggac aagccgcccg cgcccacgtc
     8161 gtggatggag ataatcgggt tttggtcgcc gagctgccag cagcggtcga tgacttcctg
     8221 cgcgcggcgt tcgatttcag ggttgccgcg ttgtacggag ttgaagtcca aagacgcatc
     8281 atttgtaccg gtatccatcg aagaagccgc accgccgccc aagccgatga gcatgcccgg
     8341 gccgcccagt tggatcagca gtgcgccttc ggggatttcg tctttgtgcg tctgctgcgc
     8401 ctgaatgctg cccaaaccgc cggcaatcat aatcggtttg tggtagccgc gaacctgacc
     8461 gtcaaatttt tcttcaaagg tgcggaagta gcccaagagg ttcgggcggc cgaattcgtt
     8521 gttgaacgcc gcaccgccaa tcgggccttc aatcatgatg tccaacggcg aggaaatgtg
     8581 ttccggcttg ccgtagtctt gttcccacgg ctgtttgagg tcggggatgt tgaggttgga
     8641 cacggtaaag ccggtcaagc ccgctttcgg acgcgaacct ttgcccgtcg caccttcgtc
     8701 gcggatttcg ccgcccgcgc ctgttgccgc gcccgcaaac ggcgcgatgg cggtcgggtg
     8761 gttgtgcgtt tccactttca tgatgatgtg ggtgtcttcc tcgtggaaac ggtagccttg
     8821 gttttccgct gcattcggat agaaacgctc gattttcgcg cctttgatca cggacgaatt
     8881 gtctttatag gcgacgaccg taccttcggg atgcgcgttg tgcgtgtcgc gtatcatgcc
     8941 gaacagggat ttcggttgtt tttcgccgtt gaggatgaaa tcggcgttga agattttgtg
     9001 gcggcagtgt tcgctgtttg cctgcgcgaa catcatcagc tccacatcgg acggattgcg
     9061 ctgcaaagcc tgatagtttt caaccagata atcgatttcg tcggcggaga gtgccaaacc
     9121 cgtttcggta ttggctttga ccaaagcctc tttaccgccg cccaaaacat cgacggtgga
     9181 gaaggtttcg gatttgagat ggtggaataa tttggatgcc gtctgaaaat cgggcagcac
     9241 ggattcggtc atgcggtcgt gcagcaacgc cgcccattgc tgtttctgtt catcggtaag
     9301 cgcaccttcc agccacaccg ccataccgcg ctcgatgcgt tcgatgcctg ccaaaccgca
     9361 gttttccgcg atattggtcg ccttggaagc ccacggcgaa atcgtaccca aacggggcgt
     9421 taccaaaaac aaatgcaaac cctcgcgcgc ttcgggcgtt tgttcaacgc tttgcgccgc
     9481 caacaaggct tgcagttttt cgacagtcgc ggcatcaagt gctttctcgc tgccggcgaa
     9541 ataccaaaat tcgctgctta atttgacttc gggcaggccg agtgcggcgg ctttttgcaa
     9601 gagtttttcg acacggaaat cggaaagggc ggtaacgccg cgcaagggca aaacaacaga
     9661 catggattcg gctctcaaat gcggttggaa aagctgtcat tatatgcgca acgggggctg
     9721 tattgcttgg gtattttggt ttaaaatgtt gacaatctga cgggggttcc cgtttttcgg
     9781 atgtgtttgt attgtttgtt caaaggaaat tttatgaaaa aaatcgaggc gattgtcaaa
     9841 ccgttcaagc tcgacgacgt gcgcgaggcg ttgacggaaa tcggcattac gggcatgacc
     9901 gtcagcgagg tcaaagggtt cggcaggcag aaggggcata cggaaatcta tcgcggtgcg
     9961 gaatacgccg tcgatttcct gcccaaggtc aaaatcgagt tggtgttggc ggatgatgcc
    10021 gtggaacgcg cgattgacgt gattgtcgag gtggcgcgtt cgggcaaaat cggcgacggc
    10081 aagatttttg tgctgccggt cgaggaggca atccgtatcc gcacgggcga acgttcggac
    10141 gcggcggttt gagcgtttgg aatggaaatg ccgtctgaaa ggctttcaga cggc
//
LOCUS       DAXDXW010000078         9842 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXDXW010000078 DAXDXW010000000
VERSION     DAXDXW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.079"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            104..1369
                     /gene="efeB"
                     /locus_tag="ACFB64_001771"
     CDS             104..1369
                     /gene="efeB"
                     /locus_tag="ACFB64_001771"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFC1210082.1"
                     /translation="MSQNQPAQPTKRNLFKTALAVGAIGAIGGYFGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPRQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPSAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKTVHLQEMRD
                     FPNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTAQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRRF
                     VEFWGRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGDITPKDSHMRLANPR
                     DPEFLKKHCLFRRAYSYSRGPASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVGKGGFLGQGLPGV"
     gene            complement(1441..1770)
                     /locus_tag="ACFB64_001772"
     CDS             complement(1441..1770)
                     /locus_tag="ACFB64_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC1210083.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAALQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(1845..3215)
                     /gene="glmU"
                     /locus_tag="ACFB64_001773"
     CDS             complement(1845..3215)
                     /gene="glmU"
                     /locus_tag="ACFB64_001773"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC1210084.1"
                     /translation="MPQNTLNTVILAAGKGTRMYSQMPKVLHCIGGKPMVERVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDAVWVEQTEQLGTGHAVKTALPHLASEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPTGLGRIIRDGSGSVTAIVEEKDASAT
                     QKTIREINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVRPVRVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     VVIEGEVELGDNVEIGANCVIKNAKIGANSKIAPFSHLEGCEVGENNRIGPYARLRPQ
                     AKLADNVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWMR
                     PEKDKQ"
     gene            complement(3270..3542)
                     /locus_tag="ACFB64_001774"
     CDS             complement(3270..3542)
                     /locus_tag="ACFB64_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC1210085.1"
                     /translation="MRKTFLILTVSAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERRAVLPDQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(3535..4248)
                     /locus_tag="ACFB64_001775"
     CDS             complement(3535..4248)
                     /locus_tag="ACFB64_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC1210086.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFHLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTKLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVAGALGLENCFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHTPPFIDASVSDMAQLARYAETLSER
                     RQNHYNTRTNPENTKECYA"
     gene            4307..5308
                     /locus_tag="ACFB64_001776"
     CDS             4307..5308
                     /locus_tag="ACFB64_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC1210087.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSVNYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANIGGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSPEV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPAHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            5402..6250
                     /locus_tag="ACFB64_001777"
     CDS             5402..6250
                     /locus_tag="ACFB64_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC1210088.1"
                     /translation="MDFKQFDFLHLISVSGWGHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSSLPLCRAQVIVGVDYNCDLKVAKEAVLKAAAEHPLSVQN
                     EERQPAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            6277..6759
                     /locus_tag="ACFB64_001778"
     CDS             6277..6759
                     /locus_tag="ACFB64_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC1210089.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEYRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLVENGGDAV
                     "
     gene            complement(6843..8411)
                     /gene="msrAB"
                     /locus_tag="ACFB64_001779"
     CDS             complement(6843..8411)
                     /gene="msrAB"
                     /locus_tag="ACFB64_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC1210090.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQAEKWAQDAKFSSANLITVASPGFLH
                     EKKDGEFQKWYAGLNYPKLPVVTDNGGTIAQNLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRNPNADLGSLKHSFYKPDTQKKDSAIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTENPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYYFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKAAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSFNMRRTEVRSRAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKGKVK"
     gene            8557..9810
                     /gene="ftsY"
                     /locus_tag="ACFB64_001780"
     CDS             8557..9810
                     /gene="ftsY"
                     /locus_tag="ACFB64_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC1210091.1"
                     /translation="MFSFFRRKKKQETPALEEAQVQETAAKVESEVAQIVGNIKEDVE
                     SLAESVKGRAESAVETVSGAVEQVKETVAEMPSEAGEAAERVESAKEAVAETVGEAVG
                     QVQEAVATTEEHKLGWAARLKQGLAKSRDKMAKSLAGVFGGGQIGEDLYEELETVLIT
                     GDMGMEATEYLMKDVRGRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPETKEPF
                     VIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGGRNNVTVI
                     SQTAGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKAIPGA
                     PHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRPVPVR
                     YIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         2027 a         2590 c         2992 g         2233 t
ORIGIN      
        1 cgccatatgt tgcagggcgg gctttcagac cgccgctatc ggttaatgga aaaacggcgt
       61 gcagggatgc ccaccctgct gcacggatat tgaaggaaac gccatgagtc aaaaccaacc
      121 cgcacaaccg accaaacgca atctgttcaa aaccgccctt gccgtcggcg caatcggcgc
      181 aatcggaggt tatttcggcg gcaaaaaaca gggcgaaacc gccgaacgca ccgccgaaag
      241 ccaacactcg ccccaagcct atccctgcta cggcgaacat caggcaggta tcgttacgcc
      301 gcggcaggcg ttttccatta tgtgcgcctt cgacgtaacc gcgcaaagtg ccaagcagct
      361 ggaaaacctg ttccgcacac tgaccgcccg catcgagttt ctcacccaag gcggagaata
      421 ccaagacggc gacgacaaac tcccgtcagc cggcagcggc attttgggca aagccttcaa
      481 ccccgacgga ttgaccgtta ccgtgggggt gggcagcagc ctgtttgacg gccggttcgg
      541 actcaaagac aaaaaaacgg ttcatttgca ggaaatgcgc gacttcccca acgataagct
      601 gcaaaaaagc tggtgcgacg gcgatttgag cctgcaaatc tgcgccttca cccccgaaac
      661 ctgccaagcc gccctgcgcg acatcatcaa acacaccgcc caaaccgccg tcatccgctg
      721 gagtatcgac gggtggcagc ctaaatccga acccggcgcg atggcggcgc gcaacctgtt
      781 gggcttccga gacggcacgg gcaaccccaa ggtttccgat cccaaaaccg ccgacgaggt
      841 tttatggacg ggcgtggccg ccaacagcct cgacgaaccg gagtgggcga aaaacggcag
      901 ctatcaggca gtccgcctta tccgccgctt tgtcgagttt tggggcagga cgccgcttca
      961 agagcaaacc gacattttcg ggcggcgaaa atacagcggg gcgccgatgg acggcaaaaa
     1021 agaagccgac caaccggatt tcgccaaaga ccccgagggt gatatcacgc ccaaagacag
     1081 ccatatgcgc ctggcgaatc cgcgcgatcc cgaattcctc aaaaaacact gcctcttccg
     1141 ccgcgcctac agctattctc gcggacccgc ctcaagcgga cagcttgatg tcgggctggt
     1201 gttcgtctgc tatcaggcaa atcttgccga cggtttcatc ttcgtgcaaa acctcctcaa
     1261 cggcgaaccg ctggaagaat acatcagccc cttcggcggc ggctatttct tcgtcttgcc
     1321 cggcgtggga aaaggcggat tcttgggaca agggctgccg ggcgtataaa cctgcctgac
     1381 ggacaaatgc cgtccgaacc ttgccaaacg ggttcggacg gcattttact gtttggacgg
     1441 tcaggctttt ttgacgaatt cggattttaa attcatcgcg ctgccgtcga ttttgcagcc
     1501 gatgttgtga tcgccttcct gcaggcgtat gcctttgacc ttcgtacctt gtttgatcgt
     1561 catcgagctg ccttttacct tgaggtcttt gatgaggatg acggtgtcgc cgttttgcag
     1621 cgccgcgccg ttggcatcgc gcacttgagc cgcaaggtcg gcggcggatt cggtttcatt
     1681 ccattcgtgg gcgcattcgg ggcagatgta ttgtccgccg tcttcatagg tgtattcgga
     1741 ggcgcattgc gggcaagggg gtaatgacat ggtttgccgt ccttatcgga tgtttgtttt
     1801 gggatgccgt ctgaaacctg aaaccggctt cagacggcac ggctttattg tttgtctttt
     1861 tccggcctca tccagccttc gatgacggtt tggcgggcgc gggcgagggc gagtttgttg
     1921 tcttcgatat tgcgggtaat cgtgctgcct gcgcctgtgg ttactttgtt gcccagtgtt
     1981 acaggggcaa ccagtacgca gttcgaaccg atgcgcactt cgtcgccgat gacggttttg
     2041 tgtttgtgca cgccgtcgta gttggcaata atcgtgcccg cgccgaagtt ggttttgctg
     2101 ccgacttcgg cgtcgcctat gtaggtgagg tggttggctt tggtgccttt gccgatggcg
     2161 gcgtttttga tttcgacgaa gttgccgacg tgtacattgt cagccagttt ggcttgcgga
     2221 cgcaggcggg cgtacgggcc gattcggttg ttttcgccga cttcgcagcc ttcgaggtgg
     2281 gagaaggggg caattttgct gtttgcgccg attttggcgt ttttgatgac gcagtttgcg
     2341 ccgatttcga cgttgtcgcc gagttcgact tcaccttcga tgacgacgtt gacgtcaatc
     2401 acgacatcct gtccgtgttt cagacggccc cgcaaatcga aacgcgccgg atcgcgcagg
     2461 gtcacgcctg ctttgagcaa ttcttgcgcc tgttcggttt ggaagatgcg ttcgagttcg
     2521 gcgagctggc gtttgttgtt cacgccggcg gcgaggtggg aggcgcgcac ccggacgggg
     2581 cggactttga tgccgtcggc aacggctttg gcgatgaggt cggttaaata atattcgcct
     2641 tgggcattgt tgctggaaag gctgttcagc cagttttcca gtttggcgtt gggcaggacg
     2701 aggatgcctg tgttgatttc gcggatggtt ttttgggtgg cacttgcgtc tttttcttcg
     2761 acaatggcgg ttacgctgcc gctgccgtcg cggatgatgc gccccaagcc tgtcgggtcg
     2821 gcgggcacgt cggtcaacag cccgacttcg ttgcctgcgg cttcgagcag ggtttcgagg
     2881 gtttcaacgt caattaaagg aacgtcgccg tacaacacca gcgtgcggcc ttcggaggca
     2941 aggtggggca gggcggtttt gacggcgtgg ccggtgccga gttgttcggt ttgttcgacc
     3001 cagacggcat cgcgtttgac agtgtctaaa acttgctctt tgccgtgtcc gacgacgacg
     3061 cagatgtttt gcggattcag cgcggcggcg gtgtcgataa cgcgctcgac catgggcttg
     3121 ccgccgatgc agtgcagcac tttgggcatt tgggaataca tgcgcgtgcc tttgccggcg
     3181 gcgaggatga cggtgtttaa agtgttttgc ggcatagcgt tttcctgtgc catgccgtct
     3241 gaagcggttt cagacggcat agggtaggtt tatcggtttt gaaactttgg tttttgccag
     3301 tgttggcgat gctcttcgtc ggcgttgttg ccggtttggt cgggcagcac ggcgcggcgt
     3361 tcgggacggt attggttgta gttcatattt ttcgagtagc tgccgtcttg gtaataaacg
     3421 ggcgtgccgg cgggatattt ttgacggacg gcggttttgc cgttgccgtc ttgataagtt
     3481 tcccacgcgc agcccgacaa aagggcggcg gagacggtca ggataaggaa ggttttacgc
     3541 ataacattct ttcgtatttt cggggtttgt gcgggtattg taatgatttt ggcggcgttc
     3601 tgacaaagtt tctgcatacc gtgccagttg cgccatatcg cttacggagg catcgataaa
     3661 gggcggcgtg tgggattttg caccgaaccg gacggttttc atgcccaatg cttttgcctg
     3721 atggaggttg tccgcgctgt cgtccaccat aatgcagcat tcgggcggta cgtccaacag
     3781 gcggcagaca ttgagatacg cttgcggatt gggtttgtac agcagtccga aatcatccgt
     3841 gccgaacagt gcgtcgaaac agttttccaa accgagtgcg ccggcaacgg cacggacgta
     3901 aaacgacggg ccgttggaaa aaaccgcctt gcgcccgttc agacggcata gggtgttttc
     3961 cgtatccgcc atgccgtgca gcttggtcag gattgcatcg atcggatggc tttcgcgtaa
     4021 aaattccgca atgtcgattt cgggatggtg gatttgcagt ccggcgagcg ttgcgccgta
     4081 acggtgccaa tagtcttgac gcaggtcgga cgcggcggat tcggagagtt tgaggcggcg
     4141 tgccatatag cgcgtcatag cgcggttgat gaggtggaag atgcctgcgt cggcatcgtg
     4201 cagcgtgttg tcgaggtcga acagccacac ggtcgggttt tcttgcattt tgaaccgtga
     4261 aaatttgtta gaatgttatt ttacagcgaa taaaggaaga ctcggaatga aacggaaaat
     4321 ttggctgctg ccgctgctgg cggtttcggc atacctgcag gcgcagacgg aagtcaggct
     4381 ggcggtgcat aagtcgttca gcctgcccaa agggctgatt gcgcgcttcg agcgggcaaa
     4441 cgatgcgaaa gtgtcgatta ttcaggcggg cggcgcgaac gaaatgctca acaaactgat
     4501 tttgagccga gccaacccga ttgccgatgc ggtgtacggt ttggacaacg ccaatatcgg
     4561 caaggcgcgg gaaatgggca ttttggcggc ggcgcaaccc gaatccgccc ccgtcgcggt
     4621 cgggctgcct tcggctttgg cggtcgatta cggctatgtg tccgtcaatt acgacaaaaa
     4681 atggtttgaa ggcaaaaagc tgcccctgcc gcaaaccctg caggatttga cccgccccga
     4741 atataaaaac ctattggtcg tgccgtcccc cgccacatct tcccccggct tgggcttcct
     4801 gatggcgaac atcggcggtc tgggcgaaga aggcgcgttt aaatggtggg cacagatgcg
     4861 gcagaacggc gtgaaggtcg ccaaaggctg gagcgaggcg tattacaccg acttttcgca
     4921 caacggcggc gcgtatccgc tggtggtcgg ttatgccgcc agccctgcgg cggaagtata
     4981 tttttccaaa ggcaaataca gcgagccgcc gacgggcaac ctgtttttaa aaggcggcgt
     5041 attccgtcag gtcgaaggcg cggcggtctt gaagggcgcg aaacagccga agctggcggc
     5101 aaaactggtg caatggctgc aaagtccgga agtgcagcag gcggttccgt ctgaaatgtg
     5161 ggtttacccc gccgtcaaaa acacgcgcct gcccgacgtg ttccgcttcg cccaagcccc
     5221 ggcgcacact accgcccccg cgcagcgcga tattgatgcg aaccagcgcg gatgggtttc
     5281 ccgttggatc aggacggttt tgaaataaaa caaacatacc gccccgcagg gcttcagacg
     5341 gcatttttac acctgtgccg attacgccgc acggggcgga tgttcgatcg agaggaaaac
     5401 aatggacttc aaacaatttg attttttaca cctgatcagt gtttccggtt gggggcatct
     5461 ggctgaaaag gcgtgggcgt tcgggctgaa ccttgccgcc gcgctgctta ttttcttggt
     5521 cgggaaatgg gcggcgaaac gcattgtcgc cgtgatgagg gcggcgatga cgcgcgcgca
     5581 ggtcgatgcc acgctgatta gttttttgtg taatgttgcc aatatcggct tattgatttt
     5641 ggtgattatt gccgcattgg gcagattggg cgtttccaca acatccgtaa ccgccttaat
     5701 cggcggcgcg ggtttggcgg tggcgttgtc cttaaaagac cagctgtcca attttgccgc
     5761 cggcgcgctg attatcctgt tccgcccgtt caaagtcggc gactttatcc gtgtcggcgg
     5821 ttttgaagga tatgtccggg aaatcaaaat ggtgcagact tctttgcgga cgaccgacaa
     5881 cgaagaagtc gtgctgccca acagcgtggt gatgggcaac agcatcgtca accgttccag
     5941 cctgccgctt tgccgcgccc aagtgatagt cggcgtcgat tacaactgcg atttgaaagt
     6001 ggcgaaagag gcggtgttga aagccgccgc cgaacacccc ttgagcgttc aaaacgaaga
     6061 gcggcagccc gccgcctaca tcaccgcctt gggcgacaat gccatcgaaa tcacattatg
     6121 ggcttgggca aacgaagcag accgctggac gctgcaatgc gacttgaacg aacaagtggt
     6181 cgaaaacctc cgcaaagtca atatcaacat cccgttcccg caacgcgaca tacacatcat
     6241 caattcttaa acgccgtctg aaaggggggg tgggaaatgg acgcgttgca caccatcgcc
     6301 cgaaacctga cgaaaaaacg tcaaaccgta agctgtgccg aatcctgcac gggcggaatg
     6361 cttgccgccg cattcacaag cgttgcaggc agttcgcaat ggttcgacca gagttttgta
     6421 acatacagca acaaagccaa agaataccgc ttgggcgtgt tgcccgaaac cctgctcgaa
     6481 cacggcgcgg tcagccgcca aaccgtctat gagatggcgc gcggcgcgaa agccgtggct
     6541 caggcggatt acgccgtcgg tatttccggc atcgccggcc cgggcggcgg cgaaagcaaa
     6601 cccgtcggca cggtttggtt cgggtttgcc tttccgggcg gaagttgcga agcaatgcgc
     6661 cgttttgacg gcaaccgcga atccgtccgc gcgcaggcgg tcgccttcgc gttggaacgg
     6721 ttggccgggc tggttgaaaa cggcggcgat gctgtctgaa caaatccccg ccggataaaa
     6781 aatgccgtct gaaacgttgc aggtttcaga cggcattttg ccgggttagg cggcggtgcg
     6841 gcttatttca ctttgccctt caacgcgccg tagcctgccg cgtccatttg ttccagcggg
     6901 atgaatttca agctcgcgcc gttgatgcag tagcgcagtc cgcctttgtc gcgggggccg
     6961 tcggggaaga cgtgtcccaa gtgcgaatcg gcggcgcggc ttctgacttc ggtgcggcgc
     7021 atattgaagc tgaaatcatc gtgttcggta acggattttg catcaatcgg gcgcgtgaag
     7081 ctcggccagc cgcagccgga atcatatttg tcggcggagc tgaacagggg ttcgccgctg
     7141 acaacgtcca cataaatgcc gggtttgaac aaatggtcgt attcgtggct gaaggcgtat
     7201 tcggtcgcgc tgttttgggt cacttggtat tgctcttcgg tcagggtgcg tttgagttcg
     7261 gcgtcactcg gttttttata cgttgccgcg tcgaagcctt tgccttgcgg tgcggctttg
     7321 gttttgcccg gcagcggttc gtcggctttg cggatgtcga tgtggcagta gccgttgggg
     7381 tttttaatca ggtagtcctg atggtattcc tcggcgtcgt agaagttttt cagcggttcg
     7441 ttttcaacaa cgaggggcag ttggtatttt tgctgctcgc gtttgagggc ggcggcgatg
     7501 acggcttttt cggcggggtc ggtgtagtac acgccgctgc ggtattgcgt gccggtgtcg
     7561 ttaccctgtt tgttgaggct ggtcggatca acgacgcgga aataatattg caggatgtcg
     7621 tccaggctga gtttgtcggc atcgtaggtc actttgacgg tctcggcatg gcccgtatgg
     7681 cggtaggaca cgtcttcgta gctcgggttt tccgtgttgc cgttggcgta gccggatacc
     7741 gcgtcaacca cgccgtcgat gcgttggaaa taggcttcca agccccagaa gcagccgccg
     7801 gcgaggtaga tggtgcgcgt gttcatgatt gctgaatcct ttttctgagt gtcgggtttg
     7861 tagaacgaat gtttcaaact gcccaaatcg gcattcgggt tgcggattaa cgccaatgcc
     7921 tgcgcttcgt tgatgctgcc tttgacgatg cgctgcacgt cgccgtcttt accgattaac
     7981 gcccaagaag gataaacgct gatattcagg ttttgggcga tcgtgccgcc gttgtcggta
     8041 acgacgggca gcttggggta gttcaaaccg gcataccatt tttgaaactc gccgtctttt
     8101 ttctcgtgca aaaagccggg ggaggcgacg gtaatcaggt tggcggagct gaattttgca
     8161 tcttgcgccc atttctcggc ctgtcccaat tcggacaaac ataaaggaca ccagctcgcc
     8221 caaaatttaa tcagcgtcgg tttgtctttt ttcaaataaa cactggcggg gcggttgtcc
     8281 gcggttttta acgtggataa agtgtgcggc acggtcgcgg ccccggcatc gacgattttg
     8341 ggcgaacaag cgcccagcgc aagcaggcag ccgaacttgg cgcaaaggga aaagaaagta
     8401 cggtgtttca ttttgatgtt tcctgtgtgg acggtttgca tgattagacg tttgagatgc
     8461 cgaaacctta cggcccggat tttcagacaa ccttgccgcg caaaatacgc tacaatacgc
     8521 cctatttcaa gtttctaaaa ttaaaaggaa aattcaatgt tcagcttctt ccgtcgcaag
     8581 aaaaaacagg aaacgccggc tctcgaggag gcccaagttc aggaaaccgc agcaaaagta
     8641 gaatctgaag ttgctcaaat agttggaaat attaaagaag atgtcgaatc tttagcagaa
     8701 agcgtcaaag ggcgggccga atctgccgtt gaaaccgtca gcggtgcggt tgaacaggta
     8761 aaggaaaccg ttgccgagat gccgtctgaa gcaggggaag cggcggaacg cgttgaatcc
     8821 gcaaaagaag ctgttgccga aaccgtcggc gaggctgtcg ggcaagttca agaagccgtt
     8881 gcgacaactg aagaacacaa gctcggttgg gctgcgcgtt tgaaacaagg cttggccaaa
     8941 tcacgcgaca aaatggcgaa atcgctggcc ggcgtgttcg gcggcggaca aatcggcgag
     9001 gatttgtacg aagagctgga aaccgtgctg attaccggcg atatgggcat ggaggccacc
     9061 gaatacctga tgaaagacgt gcgcggccgc gtcagcctca aagggctgaa agacggcaac
     9121 gaattgcgcg gcgcgttgaa agaagccttg tacgacctga ttaagccgtt ggaaaaaccg
     9181 ctggtcttgc ccgaaactaa agagcctttc gtgattatgc ttgccggtat caacggcgcg
     9241 ggcaaaacca cgtctatcgg caaactcgcc aaatatttcc aagcgcaggg caaatccgta
     9301 ttgctggcgg caggcgatac tttccgcgcc gccgcgcgcg agcagcttca ggcttggggc
     9361 gggcgcaaca atgtgaccgt gatttcacaa actgcgggcg attccgccgc cgtgtgcttc
     9421 gatgccgtcc aagccgcgaa agcgcgcggg atcgacatcg tgcttgccga caccgccggc
     9481 cgcctgccca cgcagcttca tttgatggaa gaaatcaaaa aagtgaagcg cgtgctgcaa
     9541 aaagccattc ccggcgcgcc gcacgaaatc atcgtcgtac tcgatgccaa tatcgggcaa
     9601 aacgccgtca accaagtcaa agcctttgac gacgcattgg ggctgacggg gcttatcgtt
     9661 accaaactcg acggcacggc aaaaggcggc atcctcgccg cgcttgcttc cgaccgcccc
     9721 gtccccgtcc gctacatcgg cgtgggcgaa ggcatagacg acctgcgccc gtttgacgcg
     9781 cgcgcgtttg tggacgcact gctggattga gccgaaatgc cgtccgaaaa cggcagaccg
     9841 cc
//
LOCUS       DAXDXW010000079         9780 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXDXW010000079 DAXDXW010000000
VERSION     DAXDXW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9780
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.080"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            312..875
                     /locus_tag="ACFB64_001781"
     CDS             312..875
                     /locus_tag="ACFB64_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC1210092.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            878..1426
                     /locus_tag="ACFB64_001782"
     CDS             878..1426
                     /locus_tag="ACFB64_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210093.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQEFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            1433..4648
                     /gene="carB"
                     /locus_tag="ACFB64_001783"
     CDS             1433..4648
                     /gene="carB"
                     /locus_tag="ACFB64_001783"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC1210094.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKADNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGRTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSEDKAEIRRELANPGPERMLFVADAFRAGFTPEEIHEI
                     CAIDPWFLAQIEDLMKEEKSVSDGQLQDLDYAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILSPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQAINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4849..5889)
                     /gene="queA"
                     /locus_tag="ACFB64_001784"
     CDS             complement(4849..5889)
                     /gene="queA"
                     /locus_tag="ACFB64_001784"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC1210095.1"
                     /translation="MDISDFDFTLPEHLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETVAAVEAAKARGNKAWAVGTTSMRALESAARATGYLKDGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVGAFSGMGHIRAVYRHAIEREYRFFSYGD
                     AMVLGRNEGGGL"
     gene            6006..6263
                     /locus_tag="ACFB64_001785"
     CDS             6006..6263
                     /locus_tag="ACFB64_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC1210096.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENEHNFTVVSHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            6245..6706
                     /gene="accB"
                     /locus_tag="ACFB64_001786"
     CDS             6245..6706
                     /gene="accB"
                     /locus_tag="ACFB64_001786"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC1210097.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAAAPVY
                     AAPVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAAFVEV
                     GQQVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            6820..8181
                     /gene="accC"
                     /locus_tag="ACFB64_001787"
     CDS             6820..8181
                     /gene="accC"
                     /locus_tag="ACFB64_001787"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC1210098.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKGSLHVKL
                     ADESVCIGPAASAQSYLNIPAIIAAAEVTCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDDAEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVLADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFIDEEARKKIGKACTDACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQEGGVSIHYLEHWLEARKTK
                     QDK"
     gene            8285..9172
                     /gene="prmA"
                     /locus_tag="ACFB64_001788"
     CDS             8285..9172
                     /gene="prmA"
                     /locus_tag="ACFB64_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC1210099.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDACAGTQNEQA
                     IFGEPGMPTEQIWQQSKVIALFGEHDEAAAVIDAAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGCAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGRDNAEQNNVDAQFFLP
                     DSLPQGQFDVVVANILANPLRMLGEILAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETDEGWARLSGVKR"
     gene            9190..9753
                     /gene="orn"
                     /locus_tag="ACFB64_001789"
     CDS             9190..9753
                     /gene="orn"
                     /locus_tag="ACFB64_001789"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC1210100.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDDLLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWIPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPIAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPKAEAQ"
BASE COUNT         2514 a         2780 c         2476 g         2010 t
ORIGIN      
        1 ggtggtttga aaaataactt aaatttgcaa ccgttgattt caggtcgtct gaaaaagaat
       61 gcacgaaata tcaacagcgg gaatttttca ggtagccttt attgtcaagc agatggaaca
      121 aacgccgcga gcgttttttc agacaatctt tgaactcatc ggcagagagt gtgccgcaag
      181 gcgcgcatgc ggtgggttgg ggttacaggg aaaatgaaga acgcgtgcgt gcgtaccgca
      241 cataccctac atgcgggcta cggcttgcta ggattgctcg ttatacttaa ttttgacaag
      301 gagtcaatga aatggcgata gaactaaatg gtaactggac aaaaggattt gctttagacc
      361 atcatatgca aaaaagtatt ttgttaggtt atgatgaatt agggcatcct cactttgata
      421 gcaagagaag tgtgattgga gaatgggttt atcaattaaa atatcgtaat caaacccaaa
      481 atgtttcttt gctggtagac tatattttac aaaaatttag tggtttagaa tctataaatt
      541 taattgttcc cgcccctttt acaacagagc gtattaatca accagttcaa ttaattgcca
      601 aagaactaag tgatcgcttg aatattccat attctcctat tttaagaaaa tctagctcac
      661 acacaccact caaaaatatt gaagaaaaat cagaaaaact agaaatactg aataattcca
      721 tcacaattga caatatagat ctatccaata agaatatttt agtaatcgat gatttatttg
      781 atagcggagc aacattggaa atatcaactg aaaaattgtt ttcaaaaaat gctagatctg
      841 taattgtttt agcaatgaca aagactagag gataaaaatg cgcactattt tcttttctgg
      901 ttcacgcagc atttcacgac taaatccaca aatacgagaa agaattaaca atattcttag
      961 caataatttt gatattgtta ttggagatgc taatggggct gataaagcta tccaagaatt
     1021 tctacaagaa caagattatg ctaatgtgca tatttacttt tctggcaaaa tttaccgaaa
     1081 taatgtggga aattggcagt ttgtccaagt agacagcaaa ggaactggaa gggtatttta
     1141 tacagcaaaa gataagaaaa tggcagaaat tgctgattat ggctttattt tatgggatgg
     1201 taagagtatt ggttcattaa ataatatagc agaactttta cagctaaata aaccttctct
     1261 tgtataccat tctcaaacaa aagaattctt taaaattaaa tcaagcgcag atttagagaa
     1321 tattttatct aacatagaag atgatgtttt agcttcaatt ttagaaaagg gaaatacttt
     1381 tcttaaaagc tatgtaacaa aacaaccttc tttaattcaa gagtaaccac ccatgcccaa
     1441 acgtaccgac ctaaaatcca tccttatcat cggcgccggc cctatcgtta tcggtcaggc
     1501 ctgcgaattt gactattcgg gcgcacaggc ctgcaaggct ttgcgtgaag aaggctataa
     1561 agtcattctg gtgaattcca accccgccac gatcatgacc gaccctgaaa tggcggatgt
     1621 tacctacatc gaaccgatta tgtggcagac ggtggagaag attatcgcca aggagcggcc
     1681 cgatgcgatt ctgcccacga tgggcggtca gaccgcgctg aactgtgcgc tggatttggc
     1741 gcgtaacggc gtgctggcga aatacaatgt cgagttaatc ggcgcaacgg aagacgcgat
     1801 cgacaaggcg gaagaccgcg gccgctttaa agaagcgatg gaaaaaatcg gcctctcttg
     1861 cccgaaatct tttgtctgcc acaccatgaa cgaagccttg gcggcgcaag aacaggtcgg
     1921 ctttccgacg ctgattcgtc cgtctttcac gatgggcggt tcgggcggcg gcattgccta
     1981 caataaggat gagtttttgg cgatttgcga acgcggtttc gatgcgtcgc ctacgcatga
     2041 gctgctgatt gagcagtctg tgctcggctg gaaagagtac gagatggaag tggtgcgcga
     2101 taaggcggac aactgcatca tcatctgttc gattgaaaac ttcgacccga tgggcgttca
     2161 tacgggcgac tcgattacgg ttgcgccggc gcaaacgctg acggacaagg aataccaaat
     2221 catgcgcaac gcttcgttgg cggtattgcg cgaaatcggc gtggacacgg gcggctcgaa
     2281 cgtgcagttt gcggtgaacc ctgaaaacgg cgagatgatt gtgatcgaga tgaacccgcg
     2341 cgtgagccgt tcgtccgcgc tggcttccaa agcaacgggc ttcccgattg cgaaggtggc
     2401 ggcgaagctg gcggtcggct ttacgctgga cgagttgcgc aacgacatca ccggcggccg
     2461 cacgcccgcg tcgttcgagc cttccatcga ctatgtggta accaaaatcc cgcgtttcgc
     2521 gtttgaaaaa ttccccgccg cagacgaccg cctgaccacg cagatgaaat ctgtgggcga
     2581 agtaatggcg atgggccgta ccattcagga aagcttccaa aaagccttgc gcggtttgga
     2641 aaccggcttg tgcggtttca atccgcgcag cgaagacaaa gcggaaatcc gccgcgaact
     2701 ggccaacccc ggccccgaac gtatgctgtt tgtggcagac gcgttccgcg cgggcttcac
     2761 gccggaagaa atccacgaga tctgcgccat cgacccttgg ttcttggcgc aaatcgaaga
     2821 cttgatgaag gaagaaaaat cggtttcaga cggccagttg caagatttgg attacgccgc
     2881 cctacgccgt ctgaaacgca aaggcttctc cgacaaacgt ttggcacaat tgttgaacgt
     2941 aagcgaaaaa gaagttcgcg aacaccgcta cgcgctgaag ctgcatccgg tttacaaacg
     3001 cgtcgatacc tgcgccgccg agttcgccac cgaaaccgcc tatctttact ccacttacga
     3061 agaagaatgc gaatcccgtc cttccgaccg taaaaaagtg atgattctcg gcggcggccc
     3121 gaaccgcatc ggtcagggca tcgagtttga ctactgctgc gttcacgccg cactcgccct
     3181 gcgcgaatcg ggctttgaaa cgattatggt caactgcaac cccgaaaccg tgtccaccga
     3241 cttcgacacc agcgaccgcc tctatttcga gccgctgaca ttggaagacg tgttggaaat
     3301 cgtccgcacc gaaaacccgt ggggcgtgat tgttcattac ggcggtcaaa ccccgctgaa
     3361 actcgccaac gcattggttg aaaacggcgt gaacatcatc ggcacatccg ccgacagcat
     3421 cgatgccgcc gaagaccgcg aacgcttcca aaaagtgttg aacgacttag gcctgcgcca
     3481 accgcccaac cgcatcgccc acaacgaaga agaagcactc gtcaaagccg aagaaatcgg
     3541 ttatccgctg gtcgtgcgcc cgtcttatgt tttgggcgga cgcgcgatgc agattgtcca
     3601 ctccgccgaa gccttgcaaa aatacatgcg cgaagccgtg caggtttccg aagacagccc
     3661 cgtgctgctc gacttcttcc tcaacaacgc gattgaagtc gatgtggact gcgtttcaga
     3721 cggcaaagat gtggttatcg gcggcatcat gcagcacgtc gaacaggcgg gcatccactc
     3781 cggcgactcc ggctgctcgc tgccgcccta ctccttaagc gaagaaatcc aagacgaaat
     3841 ccgccgccaa accaaagcga tggcgtacgc gctgggcgtg gtcggactga tgaacgtgca
     3901 gtttgccgtg caggacggcg tggtgttcgt gttggaagtg aacccgcgcg ccagccgtac
     3961 cgtccccttt gtctccaaag ccaccggcgt gccgctcgcc aaagtcggcg cgcgctgcat
     4021 ggcaggcatt tccctgaaag aacaaggcgt ggaaaaagaa gtcgtccccg atttctatgc
     4081 cgttaaagaa gccgtgttcc cattcatcaa attcccgggc gtggatacga ttttgagtcc
     4141 ggaaatgcgc tccaccggcg aagtgatggg cgtgggcgca agtttcggcg aagcctacta
     4201 caaagcccaa ctcggcgcgg gcgaacgcct caacccgacc ggcaaaatct tcctctccgt
     4261 gcgcgaagaa gacaaagaac gcgtcattaa aaccgccaaa aacttccaag ccttaggcta
     4321 cggcatctgc gccacgcgcg gcacggcgca atacctgacc gaacacgggc tgattgtgca
     4381 ggccatcaac aaagtccccg aaggccgccc gcacatcggc gacgcgctga aaaacggcga
     4441 aatcgcactg gtcgtaaata ccgtttccag cgatccgcaa tccgtgtccg acagccacat
     4501 catccgccaa agcgcattgc agcaacgcgt gccgcaatac accaccaccg ccggcggcga
     4561 agcgatgagc gaaggcgcga aaagccggga ccatctgggt gtgtacagcg ttcaagagct
     4621 gcacggtcgt ttgaaaaacc gcaactgatg cctgaatcag gttgaacaat gccgtctgaa
     4681 gccgttttgc ggtttcagac ggcattttgt tgcacatagc cttcactttc ttttgacctt
     4741 taagcaccct aatttggggc ggtggattat ccaactgccg gacatccata aaaataccgc
     4801 ccgaaccacg gttcagacgg catctgcctt gcaacggaca gcagcagttt aaagcccgcc
     4861 cccttcgttc cgccccaaaa ccatcgcatc tccgtagctg aagaaacggt attcacgttc
     4921 aatcgcatga cggtacacgg cgcggatatg acccataccc gaaaacgcgc cgaccaacat
     4981 cagcagcgtc gatttcggca gatgaaaatt ggtaaccagc ctgtcgacaa cattaaaacg
     5041 gtagcccggc gtgatgaaaa tatcggtgtc gccctgtccg tctttcaaat atcccgttgc
     5101 gcgcgcggca gactcgaggg cgcgcatgga agtcgtgccg accgcccagg ctttgttccc
     5161 ccgggctttt gccgcctcaa cggcggcgac ggtttcagac ggcacttcaa accattcgct
     5221 gtgcattttg tgttcttcga ttttatcgac gcgcacgggt tggaatgtcc ccgcaccgac
     5281 gtgcagggtt acttctgcgg ttaccgcgcc tttgtctttc agacggcgca aaagttcttc
     5341 cgtaaaatgc aggcccgccg tcggcgcggc gaccgcgccc tgatatttgg cataaacagt
     5401 ttgataacgg ctgtcgtcgt ccgcatcggc ggcacgttcg atataaggcg gcaggggcag
     5461 gtgtccgttc tgttccaaaa gttcgtaaac ggtttgaccg ccttcaaaac gcaggcagaa
     5521 cagttcgccc tcacgcccga ccatcacggc acggataccg ccttcaaaca ccagccccat
     5581 accgggcttg ggggacttgg acgaacggat gtgcgccaat gcggtatggt tgtccaacac
     5641 acgctcaatc agggcttcga tcctgccgcc gctgtctttc tgcccaaaca gccgcgcctt
     5701 catgactttg gtgttgttga ataccaaaac gtcgcctgcc tcgacataat cgggcaaatc
     5761 gccaaacacc cggtcttgca gcggcatatc gggcagcgcg accaaaagcc ggctgctgcc
     5821 gcgcacctcg ggcggatgct gggcaatcag gtgttcgggc agggtaaagt caaaatctga
     5881 aatatccatt tttacactct cgttcgggca agccgccatt atacgcactt tagccctttt
     5941 tcagacggca tctttgcccg aaaaaccaac agattagaat aaacactctt gactcggggc
     6001 atcttgtgcg caaaatcaaa cttcctgcac atttccccca aaaaccgccg ttttttgata
     6061 ttttactgga catttaccga caacttcggg aaaatgaaca caatttcacg gtcgtttccc
     6121 accacaggaa aaccgtatcc gaacaccatt ccgcccggtt tgcgccgttg ccgcaagccg
     6181 gctgttttct gaaaaaccaa cgcaacaacc cgccggaaca ccggcagcct ttaaaggaac
     6241 agaaatggat ttgcgcaaat taaaaaaact gattgatttg gttgaagaat cgggtatcgc
     6301 cgaaatcgaa gtaaccgaag gcgaggaaaa agtccgcatt acccgtacca ttgcagctgc
     6361 ggcagctccc gtttacgccg cgcccgtacc tgccgccgcg ccggccgtaa cgccggccgc
     6421 cgcacccgtt gcggcatccg cgcccgccgc cgcgccggcc gcccgcgatt tgtccgacgc
     6481 tcaaaaatcg cctatggtcg gcacgttcta ccgcgcgccc ggcccgaatg ccgccgcatt
     6541 tgtcgaagtc ggtcaacagg tcaaagccgg cgacacgctg tgcatcatcg aagcgatgaa
     6601 gctgatgaac gaaatcgaag ccgaaaaatc cggcacggtc aaagaaattc tggtcgaaaa
     6661 cggtacgccc gtcgaattcg gcgaaccgct cttcatcatc ggataatcct gttttcagac
     6721 ggcataaact tccgatgccg tctgaaatgc tttccccccc ttcagcgttc ccgcaccctt
     6781 ttttacggac gggttgccgg aaccgcagga aaggtcatca tgctgaaaaa agttttaatc
     6841 gccaaccggg gcgaaatcgc cttgcgcgta ctccgtgcct gccgcgaaat gggcattgcc
     6901 accgttgccg tgcattccga agccgacaaa ggcagcctgc acgtcaaact cgccgacgaa
     6961 tccgtgtgca tcggtcctgc cgcttctgcg caaagttatc tgaacattcc cgccatcatt
     7021 gccgccgccg aagtaacctg cgcggacgct gtccacccgg gctacggctt ccttgccgaa
     7081 aacgccgatt tcgccgaaca ggtcgaacag tccggcttta ccttcatcgg cccgaaaccc
     7141 gacaccatcc gcctgatggg cgacaaagtc tccgccaaac acgcgatgat agcggcaggc
     7201 gtgccctgcg tccccggctc tgacggcgca ttgcccgacg atgatgccga aatcctcaaa
     7261 atcgccgaca aagtcggtta tcccgtgatt atcaaagcat cgggcggcgg cggcgggcgc
     7321 ggtatgcgcg tggtcgagaa aaaagaagac ctcctccagt ctgtcgaaat gaccaaggct
     7381 gaagcgggcg cggcgttcgg caacccgatg gtttatatgg aacgctacct gcaacgcccg
     7441 cgccacgtcg aaatccaagt gcttgccgac gaacacggca acgccgtcta tcttgccgag
     7501 cgcgactgtt cgctgcaacg ccgccaccag aaagtcatcg aggaagcacc cgccccgttt
     7561 atcgatgaag aggcacgcaa aaaaatcggc aaagcctgta ccgacgcgtg caaacgcatc
     7621 ggctaccggg gcgcgggcac gtttgaattt ttatacgaag acggcgaatt tttctttatc
     7681 gagatgaaca cgcgcgttca ggtcgagcat ccggttaccg aactcattac cggcgtggac
     7741 atcgtccaag agcaactccg catcgcatcc ggcctgccgc tgcaatacaa acagaaagac
     7801 atcaaaatcg aaggccacgc cttcgagtgc cgcatcaatg ccgaagaccc gtacaacttc
     7861 atccccagcc cgggcccgat tgaaagctgc cacctgcccg gcggcttcgg catccgcgtg
     7921 gacagccaca tctaccaagg ctaccgcatc ccgccgtact acgacagcct gatcggcaaa
     7981 atctgcgtac acggcaaaac gcgtgaacag gcaatggcga aaatgcgcgt cgcactcgcc
     8041 gagctggcgg taaccggcat caaaaccaat acgccgttgc accgcgacct gttcgccgat
     8101 gcgggtttcc aagaaggcgg agtgagcatc cattacctgg agcactggct ggaagcgcgc
     8161 aaaaccaaac aggacaaata agccgccgcc gatatgccgt ctgaagccgc ccttccgcgt
     8221 tcagacggca tttcccttgc cccgccgtct gaaaccgatt tcgatatttc caagaaagcc
     8281 cgttatgccc taccaacaaa tcaccgtcaa cgtcaacgat gccgtcgccg aacgcctcgc
     8341 cgacgcgctg atggaacacg gtgcactctc cgccgccatc gaagatgcct gcgccggcac
     8401 gcaaaacgaa caggcgattt tcggcgaacc cggtatgccc accgaacaaa tctggcagca
     8461 gagcaaagtc atcgccctgt tcggcgaaca cgacgaagcc gccgccgtca tcgatgccgc
     8521 cgcacaagaa tgcgggttaa aagacttggc atacaccggc gaaaccatcg aagaccaaga
     8581 ctgggtgcgc ctcacgcaat cgcaattcga ccccatccga atttccgacc gcctgtggat
     8641 taccccctct tggcacgaag cccccgaagg ctgcgccgtc aacctccgcc tcgatcccgg
     8701 actcgccttc ggcaccggca gccacccgac cacgcgcctc tgcctcaaat ggctggatac
     8761 gcaactcaaa aacggcgaaa gcgtcctcga ctacggctgc ggttcgggca tcctgaccat
     8821 cgccgccctc aaactcggtg caggttccgc cgtcggcgtg gatattgacg aacaggccgt
     8881 ccgcgccggc agggacaacg ccgaacagaa caacgtcgat gcacaattct tcctgcccga
     8941 cagtctgcct caagggcaat tcgatgtcgt cgtcgccaac atcctcgcca accctttgcg
     9001 tatgctcggc gaaatacttg cagcccgcac caaacagggc ggacgcatcg tgttgtccgg
     9061 tttgttggat gaacaggccg aagaactcgg cggcatttac agccaatggt tcgatctcga
     9121 cccggcggaa actgacgaag gctgggcgcg cttgagcggc gtaaaacgct gaaacggaaa
     9181 ggaaacaccg tgcaggacaa aaacaacctc tgctggctcg atatggaaat gacggggctg
     9241 aatcccgaaa ccgaccgcat tatcgaagtc gcgatgatta ttaccgactc ggatttgaat
     9301 gtgttggcgc aatccgaagt ttacgccatc caccaaagcg acgatctgct cgacaatatg
     9361 gacgagtgga acaccgccac acacggcagg acggggctga cgcagcgcgt gcgcgaatcg
     9421 tcgcataccg aagccgaagt cgaacagaaa ctgctggact ttatgtcgga atggataccc
     9481 ggacgcgcca caccgatgtg cggcaactcc atccaccaag accggcgttt tatggtcaaa
     9541 tatatgccga aactggaaaa ctacttccac taccgcaacc tcgacgtttc cacgctgaaa
     9601 gaactcgcca aacgctggaa tccgcccatt gccaaaagcg tcgtcaaacg cggttcgcac
     9661 aaggcattgg acgacatttt ggagagcatc gaagaaatgc gccactaccg cgaacacttc
     9721 ctgatttccg ccccgaaagc cgaagcgcaa taagaaacaa acaatgccgt ctgaaacgct
//
LOCUS       DAXDXW010000080         9766 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXDXW010000080 DAXDXW010000000
VERSION     DAXDXW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.081"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            491..2026
                     /locus_tag="ACFB64_001790"
     CDS             491..2026
                     /locus_tag="ACFB64_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC1210101.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIIGGRLPELRRHLNALSSIRVGGLWTACVVFTVVMLGYMLYQ
                     DTAGLLEKNYGDYPDGFLNIFGWGMSAALIMFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     GEK"
     gene            2023..2115
                     /locus_tag="ACFB64_001791"
     CDS             2023..2115
                     /locus_tag="ACFB64_001791"
                     /inference="COORDINATES: protein motif:HMM:NF028261.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC1210102.1"
                     /translation="MSTSAIVMMVAAIAVIWGGLLLSLLRLPEE"
     gene            complement(2151..2591)
                     /locus_tag="ACFB64_001792"
     CDS             complement(2151..2591)
                     /locus_tag="ACFB64_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210103.1"
                     /translation="MKHAPIILLLTASSAALLSAGCTALPSDRPPLRTLPKSAPIQPA
                     NPYSRPPSGTPDGAFSTRASNGRVLKSIVKNGVFDRFVDIYHPNGKLHSHTPVENGVA
                     QGYTEQGILRTRILYRGTGISSERKLWMRRARWNANGSRKTNAV"
     gene            complement(2785..4005)
                     /gene="lysA"
                     /locus_tag="ACFB64_001793"
     CDS             complement(2785..4005)
                     /gene="lysA"
                     /locus_tag="ACFB64_001793"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC1210104.1"
                     /translation="MTLFCEQVAYPRLAEEFGTPLYVYSQSALTGAFENYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKIAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYKDEGVPDLGAYARAVQKLMGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNTRNRAAEVLVDGGGYKLIRRR
                     ETLEQQMANELACL"
     gene            complement(4016..4186)
                     /locus_tag="ACFB64_001794"
     CDS             complement(4016..4186)
                     /locus_tag="ACFB64_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC1210105.1"
                     /translation="MKYGVFFAAATALLLSACGYKGGLYLPKEGDTARFGAIQTGLQL
                     QGKPQSAPPTQK"
     gene            4257..4580
                     /gene="cyaY"
                     /locus_tag="ACFB64_001795"
     CDS             4257..4580
                     /gene="cyaY"
                     /locus_tag="ACFB64_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC1210106.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIAEL"
     gene            4613..5032
                     /locus_tag="ACFB64_001796"
     CDS             4613..5032
                     /locus_tag="ACFB64_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFC1210107.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGNTGY
                     FYAASQEGILDALHIYNVEDVSDKHIPNHVLILWDGACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKPT"
     gene            5054..5560
                     /gene="luxS"
                     /locus_tag="ACFB64_001797"
     CDS             5054..5560
                     /gene="luxS"
                     /locus_tag="ACFB64_001797"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC1210108.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGAGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYLMHSLAEAQQIAQNVLARKVAVNRNGDLA
                     LDESLLNA"
     gene            5704..8496
                     /gene="polA"
                     /locus_tag="ACFB64_001798"
     CDS             5704..8496
                     /gene="polA"
                     /locus_tag="ACFB64_001798"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC1210109.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALIGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVMEHAP
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHTELSDGIESLRRTAPKWAQLAVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEIPSEKRAEKATAPEKLDYQA
                     VTTEAQFAALLDKLAKADTIGIDTETTSLDAMNAELVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNNIAGDAMLASYI
                     IESHLGHGLDELSGRWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEARLRAQMDDKQLEMYEKMELPVAQVLFEMECNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQSGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMINVRNWLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
     gene            9058..9753
                     /locus_tag="ACFB64_001799"
     CDS             9058..9753
                     /locus_tag="ACFB64_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC1210110.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     INGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVNISLLKRINNIVAKDDSLVPV
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPRFNEKLSAFYRTGDDTDISKFVYQNC
                     ISGIDYFGADEDI"
BASE COUNT         2276 a         2551 c         2640 g         2299 t
ORIGIN      
        1 ggccgccttg tcctgatttt tgttaatccg ctatatttcc cgccatccca aaaacgaaga
       61 gcggcaggaa tttatcggaa aaacagcaac ctttccgccg tcattcccgc gaaagcggga
      121 atctaggttc gtccggtttc ggttatttcc gatagattcc tgccgcgttg ggggtctgga
      181 ttcccgcctg cgcgggaatg acgggacttt aggtttctgt ttttgttttt ctgtttttgc
      241 gggaatgacg gggaagttgg cggttgtttc ttatggagtg gtttttgtat ttggataaaa
      301 aatttttcgt tttcaagcct tcaccgcttg ccatcggcgt taaaattttt tacgataagc
      361 acatagattg taaacaatcg gccacaagcc ggtttgtttt ttcagaagac attatccctg
      421 tcagacgctg tttctatata tgtttgccta taacggcttg tttttaataa ataattcaag
      481 aggtatcaac gtgtctgatt ccaaaacgaa agaacgcgcc acattcggca cgcgccgcgc
      541 gtttatgatt gccgccatcg ggtccgccgt cggcttgggc aatatttggc gtttccccta
      601 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      661 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc ggggttctgc
      721 gcccttggct ttccgccgcc tcggacgctg gttcgagccg gtcggctggt ggaacgtgat
      781 gaccaatatc gtcatctgca tctattacgc agtgattatc ggttgggcgg caagctatac
      841 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaggactt
      901 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggccc
      961 tttggcgggc gtgtgggttt ttaccgccgc cattatggct ttgggcgtgc aaaagggcgt
     1021 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
     1081 catttcgctg acgctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
     1141 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
     1201 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
     1261 gggcggaacg gggctggtgg tcggttttgc caacagcagc ttcgaactgc tcgcgggcat
     1321 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggcaaggcgg tcaacgaggt
     1381 tgcctccggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcgcc
     1441 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtgttcgccg gcgttacgtc
     1501 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1561 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1621 tacggcgacg gggctgcctg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1681 tgttgccgcc ggctttgttt atgttgccgc catcatcatc ggcggcaggt tgccggaatt
     1741 acgcaggcac ctgaacgcct tgtcctccat ccgcgtcggc ggcttgtgga cggcctgcgt
     1801 cgtgtttacc gtcgtgatgc tcggctatat gctgtatcag gacaccgccg gattgttgga
     1861 aaaaaactac ggcgattatc cggatggttt cctcaatatt ttcggctggg ggatgtcggc
     1921 ggcgttaatc atgttcgggc tgctgctgtc gctgctgcct tggaaacacg gtcaggattt
     1981 taatgtcaaa gacgaacacg aacatgaaca aggaggagaa aaatgagtac ttccgccatt
     2041 gtgatgatgg ttgccgccat cgcggtgatt tggggcgggc tgctgctttc cctgttaagg
     2101 ctgccggaag agtaagcctt tagaacgtta aaaatgccgt ctgaaccgct tcagacggca
     2161 tttgttttac ggctgccatt cgcgttccac cttgcccgac gcatccaaag tttgcgctcg
     2221 gacgatatgc ccgtcccccg gtacaggatg cgcgtccgca atatgccctg ttcggtatag
     2281 ccttgcgcca cgccgttttc aaccggcgtg tgcgaatgca gtttgccgtt tggatggtaa
     2341 atatcgacaa agcggtcgaa cacgccgttt ttgacgatgc ttttcaagac gcgcccgttt
     2401 gaggcgcggg tgctgaatgc gccgtccggc gtgcctgaag gcggccggct gtatgggttg
     2461 gcgggttgga tgggcgcgct tttcggcagg gtgcgaagcg gcggccggtc agagggaagg
     2521 gcggtgcagc ccgccgaaag tagggcggcg gacgaagcgg taagcaggag gataatcgga
     2581 gcgtgcttca tttcaattcc ttagatattg caccccataa aaggggcgcg taattttttt
     2641 caacaattcc cttcgtcagt cgggcgagac ggggatttgt tgcgtcgtca tttgaaaaca
     2701 ggctgtgttt gaatattccg ccaagatgtc actttaaacg gacaatgccg tctgaaggct
     2761 tcagacggca ttttgatgtt cggcttatag gcaggcgagt tcgtttgcca tttgctgttc
     2821 caaggtttcg cgccggcgga tgagtttgta tccgccgccg tcaaccaaca cctccgccgc
     2881 acggttgcgc gtgttgtaat tgctagccat actggccccg tatgcgcccg cgctgcggat
     2941 aagcagcaaa tcgccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt
     3001 ttcacaaatc gggccgacga tgttggcggt cagaggctca atgtttttgg tttcaaccgc
     3061 ttcgatgtgg tggtaggcat cgtatagggc tgggcgcatc aaatcgttca tcgccgcatc
     3121 gaccatcaca aagtttttct cttcaccgtg tttgacaaat tcgacgcgcg tcagcaatgc
     3181 acctgcgttg ccgaccaagc tgcggcctgg ctcaagaatg agtttcagac ggcgtgtccc
     3241 catcagtttt tgaaccgctc gggcatacgc acccaaatcg gggacgcctt cgtctttgta
     3301 aacaatgccg acgccgccgc ctaagtccaa atgttccaaa acaatgcctt cggcggcaag
     3361 agcgtcaacc aaaatcaaaa tgcgttcgca ggcttcgacc agtgggctta agtcggtcag
     3421 ttgcgaaccg atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcatg
     3481 gcggtaggct tcgagcgcgt cggcgtaggc gatgccgaat ttgttggctt tcagacctgt
     3541 ggagatgtag ggatgggttt ttgcatcgac atcgggattg acgcgcaggg agacgggcgc
     3601 ggttttgccc aaacgcgcgg caattttctg aatgcggtcg atttcgggga tgctttccat
     3661 attgaagcat tttacgccgg cattcagcgc gaactcgatt tccgcctcgc ttttgcctac
     3721 gccggaaaaa atcgttttcg ccgcatcgcc gcctgccgcc aaaacgcgtg ccaattcgcc
     3781 gcccgacaca atgtcaaaac cgctgcccaa agaagcaaag tgtttgataa tgctcaggtt
     3841 gccgtttgcc ttgacggcgt agcagacaag cgggttcaaa gcggcaaagg cggtttgata
     3901 gttttcaaat gctccggtca gcgcggattg gctgtacaca taaagcggcg tgccgaattc
     3961 ttcggcaagg cgggggtagg cgacttgttc gcaaaacagg gtcatatttt cgttttcatt
     4021 tttgggttgg tggggcggat tgcggtttgc cttgaagctg caaaccggtt tggattgcgc
     4081 cgaaacgcgc cgtatcgcct tctttgggca ggtagaggcc gcctttgtaa ccgcaggcgg
     4141 aaagcaggag ggcggttgcc gccgcaaaaa atacgccgta tttcatcggt aaacttcctt
     4201 cataagcgcg aatgtggcaa gattcggcat cttaaacaaa aaacacgcaa aaagctatga
     4261 tgaccgaaag cgagtttatc cgcgcgagcg aagcattatt tgaacacatc gaagaccaaa
     4321 tcgacgaaaa cggctgggat ttcgactgcc ggtttgccgg aaacgtcctg accatcgaag
     4381 ccggagacgg cacgcaaatc atcgtcaacc gccacacgcc caaccaagaa ttgtggattg
     4441 ccgcaaaaag cggcggctac catttcgccg aacaaaacgg caaatggctg gcaacgcgcg
     4501 acagccgcga tttttacgac gttttaaacg aagccctgag cgcggcttcg ggcgaagcgg
     4561 ttgagattgc cgaattgtga ttcaattttc gatataaaaa gattgttacc caatggcaca
     4621 attacctcta tatcggactg ccgaaatcgg aaattttact gtcggcacgc ctaaagtttt
     4681 agaatcattt tccaaacata tcccttatgg tgttgtcttt gaagatgatg gcaacacggg
     4741 ctacttctat gccgcttcgc aagaggggat tttggatgcc ttgcacatct acaatgtcga
     4801 agatgtatcc gacaaacata tccccaatca tgtcttgatt ttatgggatg gcgcctgcac
     4861 catagccgca ttgtgtatca acgactacat ccatgccgtc tatgattttg tcgaacaggc
     4921 aggatattgc cgcaacggct tccctgaagc aggcggcgaa tgggtaaaag tcgaaaaccg
     4981 cgtcttggac gatgaattgc tggacaaaat cctatcccga aaacctacat aacccgcaca
     5041 aaaggatacc caaatgcccc tgttagacag tttcaaagtc gatcacaccc gtatgcacgc
     5101 ccctgccgta cgcgtggcga aaaccatgac cacgcccaaa ggcgacacca ttactgtatt
     5161 cgacctgcgc ttttgcatcc ccaataaaga aatcctgccc gaaaaaggca tccacacgct
     5221 ggagcatttg tttgccggct ttatgcgcga ccacttgaac ggcgccggcg tggaaatcat
     5281 cgacatttcc ccgatgggct gccgcaccgg tttttatatg agccttatcg gcacgccttc
     5341 cgaacagcag gttgccgatg cgtggttggc ttcgatgcag gatgttttga atgtcaaaga
     5401 ccaaagtaaa atccccgagt tgaacgaata ccaatgcggc acttatctga tgcactcgct
     5461 cgccgaagcg caacaaatcg cgcaaaacgt gttggcgcgc aaagtggcgg ttaaccgaaa
     5521 cggcgatttg gctttggatg aaagcctgct gaacgcttaa tccgccgcaa atgctgtctg
     5581 aacaagggtt tcagacggca tttgcctttt ccgttataat ccggggttgt acgggggcgg
     5641 gttttaagcc ggcatcgtcc ttccctattt ttctgtccct tatcggtttc aagcgggttt
     5701 tttatgtcca acagacctac actcctcctc gttgacggct cttcctatct ctaccgcgcg
     5761 tatcacgcga tggggcaaaa cctgactgcg cctgacggtg cgccgacggg tgcgctgtat
     5821 ggcgtattga atatgttgcg ccgtttgcgg tcggaatatc cgcacgatta ttgcgcggtg
     5881 gtttttgatg cgaaaggcaa aaatttccgc catcaaatgt ttgaagaata caaggcgacg
     5941 cgcccgccga tgccggacga tttgcgcccg caggcggaag ccctgccgga tttggtgcgc
     6001 ctgacgggct ggccggtatt ggtgattggg caagtggagg cggacgatgt gatcggcacg
     6061 ctggcgaaac agggggcgga acatggtttg cgagtcattg tttcgacggg cgataaggat
     6121 atggcgcagt tggtggatga gcgcgttacg ctggtgaaca cgatgagcgg agaaacgctg
     6181 gatattgaag gcgtgaaggc aaaattcggc gttcgccccg accaaatccg cgattatctc
     6241 gcgctgatcg gcgacaaggt ggacaacgta ccgggcgtgg aaaaatgcgg cccgaaaacg
     6301 gcggtgaaat ggctggaagc ctacggttcg ttgcaaggcg tgatggaaca cgctccggaa
     6361 atcaaaggca aggtcggcga aaacctgcaa gccgcgctgc cccaactgcc gctgtcgtat
     6421 gatttggtca cgattaaaac cgatgtggac ttgcacaccg agctttcaga cggcatcgaa
     6481 agcctgcgcc gtaccgcgcc gaaatgggcg cagcttgcgg ttgatttcaa acgctggggc
     6541 ttccgtactt ggctgaaaga agcggaatca aacatgaata ccggctcgac cgatgatttg
     6601 ttcggtagcg acagcatcgg cgagcaggcg gctttgaatg cggaaatacc gtctgaaaaa
     6661 cgagctgaaa aagccaccgc ccctgaaaaa ctggattatc aagccgttac caccgaagct
     6721 cagtttgccg ccttgttgga caaactagcg aaggcggaca caatcggcat tgatacggaa
     6781 accacgtcat tagatgcgat gaatgccgag ctggtcggca tcagcattgc gttccaagca
     6841 ggcgaagcgg tttacatccc cgtaggccac agcctgactg ccgcgcccga acagcttgat
     6901 ttgcaagacg tattaggccg tctgaagccg catttggaaa accccgccct gaaaaaaatc
     6961 ggtcaaaacc tcaaatacga ccaacacgtt ttcgccaact acggcatcgc gttaaacaac
     7021 attgccggcg acgcgatgct cgcttcctac atcatcgaga gccatctcgg acacggcttg
     7081 gacgaattgt ccggacgctg gctcggttta gaaaccatta cctacgaatc gctgtgcggc
     7141 aaaggcgcga agcaaatcag ttttgccgat gtcgccatcg ggcaggcgac cgaatacgcc
     7201 gcccaagatg ccgatttcgc cctgcgcctc gaagcgcgcc tgcgcgcgca aatggacgac
     7261 aaacaacttg aaatgtatga aaaaatggag cttcccgtcg cgcaggtatt gtttgaaatg
     7321 gaatgcaacg gcgtgcaaat cgaccgcgcc gaactcgccc gccaaagcgc ggaactcggc
     7381 gcggagctga tgaagctcga acaggaagcc tacgccgctg caggtcaacc gtttaacctc
     7441 aactcgccca aacagctgca agaaatcctg ttcgacaaaa tgggcatccc gacaaaaggg
     7501 ctgaaaaaaa ccgccaaagg cggcatttcc accaacgaag ccgtgctcga acagctcgcg
     7561 cccgactacc ccctgcccaa aatcatcctg caaaaccgca gcctggcgaa actcaaatcc
     7621 acctacaccg acaaactgcc cgaaatgatt tcgccccggg ataaccgcgt gcataccacc
     7681 tatgcccaag ccgtcgccat taccggccgc ctcgccagca acaaccccaa cctgcaaaac
     7741 atccccattc gcaccgccga aggccgccgc gtgcgccgcg ccttcactgc accgccgggc
     7801 agcgtcatcg tttccgccga ctattcccaa atcgagctgc gcattatggc gcacctctcc
     7861 ggtgacaaaa ccctcattgc cgcatttcaa agcggcgaag acgtacaccg ccgcaccgcc
     7921 gccgaagtgt tcggcactgc gcccgaaaac gtctcgcccg agcaacgccg ctacgccaaa
     7981 accatcaact tcggcttaat ctacggtatg gggcaatacg gtttggcaaa atcattgggc
     8041 atcgacaacc tttccgccaa aaactttatc gaccgctact tcgcccgtta tcccggcgtc
     8101 gccgaataca tgcagcgcac caaagaacaa gccgccgccc aaggctacgt cgaaaccctg
     8161 ttcggcagaa ggctctattt gcccgacatc cgcaacaaaa acgccaacgc ccgcgcagga
     8221 gccgagcgtg ccgccatcaa cgcccccatg caaggcaccg cttccgacct catcaaacgc
     8281 gccatgataa acgtgcgaaa ctggctttca gacggcatcg gcagcaaact ggtcatgcag
     8341 gtgcatgacg aactggtgct ggaagtcgtt gaaaccgaac tggattttgt caaagaaaaa
     8401 ctgccgcaga ttatggcgaa agtggacggc ggattattgg atgtaccgct ggtggctgag
     8461 gttggcgtag gggagaattg ggaagaggca cattgagaat gcacaagtat tgaactgtta
     8521 cgttttgatt tatcaaaatt taaggaagta aaatacccga ttcgaatgat tggaaatcaa
     8581 tagcaagccg taggtcggat acttgtatcc gacaaaagcc tgccatctca aatagcagtc
     8641 ggattcgaga atccgacctg ccaaaccggg cgcggacgct ccggtcggca gttagcgcac
     8701 aaatcgaaca gaacatcaca aaaaagcctg attcggattt tccaatcggg cttttttgcg
     8761 cccgttttgt catcccgtga aatatccgca taacaaaaat aatgcaaaat catgacaaaa
     8821 ccggcgcgag gttacacaaa cggatgaaat caaccgatat tcaaacacag ccatttttag
     8881 cgcattttca gcgtatcttt atgcggaaaa tttcgtgaac aggttttttg cacaggcttc
     8941 cgccgcgttt cgcgggatgg gaaaccgtat tagaaaacgg acgcacgcaa cataaacccc
     9001 gaaagtgatg ataagatgat gatttaacgt actgctttaa ttatttaagg aattatcgtg
     9061 tttcccgaca aatacaagtt gagtttggaa gaaaatattt ttctggcaaa gaaagtattg
     9121 gttgctcaaa ttcacaacct cagccgtttt gagaattgtc agacgacctt gttgcagacc
     9181 gagcaaatta tcaatggcaa aaatgtagcc tccgcgtcac tggaagacat ccaaaccatc
     9241 ttgaacctga aacgtgccta tcaatatgtg atttcgcata tttcaaacgg cgaaccggtc
     9301 aatatttcac tccttaaaag aatcaacaac attgttgcca aggacgattc tttggtaccc
     9361 gtggatttcc gtaccggttc ggtcggcgta acgctattgg acggttcccg tcatgccccg
     9421 aatccggtga aggaaattga agtggcccgc gtgttgcaaa atatcggact gcaaagcggt
     9481 tcgacgacgg aagcagccgt tcgtttcatg ctctattgta tgcggcagca ggttttttgg
     9541 gacggcaaca aacggacggc aaccctattt gccaacggcc tgatgatggc ggggggctgc
     9601 ggtatcctgg aaatctccga aatgcagatg ccgcgattca atgaaaaact atccgcattc
     9661 tatcgcaccg gcgacgatac cgatatttcc aagtttgtgt atcaaaattg tatatcgggc
     9721 atagactatt tcggcgcgga cgaagatata tagtggatta aattta
//
LOCUS       DAXDXW010000081         9670 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXDXW010000081 DAXDXW010000000
VERSION     DAXDXW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9670
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.082"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(1..253)
                     /locus_tag="ACFB64_001800"
                     /pseudo
     CDS             complement(1..253)
                     /locus_tag="ACFB64_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(280..609)
                     /locus_tag="ACFB64_001801"
                     /pseudo
     CDS             complement(280..609)
                     /locus_tag="ACFB64_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147169.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            613..921
                     /locus_tag="ACFB64_001802"
                     /pseudo
     CDS             613..921
                     /locus_tag="ACFB64_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(996..3050)
                     /gene="metG"
                     /locus_tag="ACFB64_001803"
     CDS             complement(996..3050)
                     /gene="metG"
                     /locus_tag="ACFB64_001803"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC1210111.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVRKEHLADFTGFFIGYDNYYSTHSTENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELFNPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGGETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGIDFDEYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRPKDVSGSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AARFLNLDAITWKNTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIQTASAPVE
                     EGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIAA
                     SYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGDK
                     VG"
     gene            complement(3166..5004)
                     /gene="glmS"
                     /locus_tag="ACFB64_001804"
     CDS             complement(3166..5004)
                     /gene="glmS"
                     /locus_tag="ACFB64_001804"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC1210112.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVYSDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGKLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSPASLEPGPHSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSVCNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVVLFGLAVTLAKVRGVVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNAAEGVHVI
                     RAPRHVGELSPVVHTVPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            5276..5782
                     /locus_tag="ACFB64_001805"
                     /pseudo
     CDS             5276..5782
                     /locus_tag="ACFB64_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6086..7411)
                     /locus_tag="ACFB64_001806"
     CDS             complement(6086..7411)
                     /locus_tag="ACFB64_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC1210113.1"
                     /translation="MKKHLLRSALYGIAAAILAACQSRSIQTFPQPDTSVINGPDRPA
                     GIPDPAGTTVAGGGAVYTVVPHLSMPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKRFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTERARFPIY
                     GIPDDFISVPLPAGLRGGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFF
                     RELAGSGNEGPVGALGTPLMGEYAGAIDRHYITLGAPLFVATAHPVTRKVLNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            7644..8464
                     /gene="efeU"
                     /locus_tag="ACFB64_001807"
                     /pseudo
     CDS             7644..8464
                     /gene="efeU"
                     /locus_tag="ACFB64_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
     gene            8461..9627
                     /gene="efeO"
                     /locus_tag="ACFB64_001808"
     CDS             8461..9627
                     /gene="efeO"
                     /locus_tag="ACFB64_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC1210114.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGETQSANEGGS
                     VGIAVNDNACEPMNLTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVADSGFKDTANEADLEKLPQPLADYKAYVQGEV
                     KELAAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDACEDDFK
                     DGAKDAGFTGFHRIEHALWVEKDVSGVKETAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEAAGSKISGEEDRYSHTDLSDFQANADGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLSEADRKALQAPINALAEDLAQLRGILGLK"
BASE COUNT         1972 a         2412 c         2979 g         2307 t
ORIGIN      
        1 ttgtcgcgct taaccggctg tccgcagaac cattttaccg aaccgtcttg acgcttggcc
       61 cacagggaga gttttttgcc tttgatttct ttgtttacgc cggttgaagc catttgggcg
      121 gtgacgacgc cttttgcgac ttcaactttc tgaacatatt tgcctttgat tgttgaagcg
      181 gatgccacgc cggcagcgcc gttgtctttg ggccattcgc cgttttcagg taatattcgg
      241 tgagggctga tttatttgaa atttaaggcc taatttgcct tattcggcag atgacgtatc
      301 acggcaggtt gacggcaggt gcttggtttc gatttttttg gtgccgtcgg ctttgacgtc
      361 gtcggcgtcg gctttggtgc gcgtaaccgg ctgtccgcag aaccatttta ccgaaccgtt
      421 ttcacgcctg ccccacaggg atagtctttt gtctttgatt tcattgttta cgccgcttgg
      481 tttcatttgg gcggtaacga cgccgtttgc gaccgtaacg ctttcaacat atttgccttt
      541 gatgtcggcg ggggatgcca cgccggcatt accgttgttt gcgggccatt tgccgttatt
      601 cgggtaatgt ccggcgaaaa cgagttgcgg cagatgaagc ccggtgcggt tttgattaat
      661 tgcgggcgcg gcgggccggt ggatgaaaac gcgctgcctg ccgcactcaa atacgggcag
      721 atcggcgggg cgggcgcgga tgttttgacg caagaaccgc cgaggggcgg caatcccttg
      781 ctgaatgcac gattacccaa tctgattgtt acgccgcata ccgcgtgggc gagccgcgag
      841 gctttggatc ggttgtttga aatattgttg gcgaacattc acgcctttgt gaaaggcgag
      901 gcgcaaaacc gcgtggtttg aacctgtcgg gattgcggaa aaaaacgccg tctgaacgcc
      961 caaggggttc agacggcgtt ttttgagatt tctgcctaac cgactttgtc gcccggctga
     1021 gcgcccgcat ccacgtcaag cagcttcagt ttgccgtctg ccgtggcggc ggagaggatc
     1081 ataccttcgg atacgccgaa ttttgccatt ttgcgtgggg cgaagttggc gactgcgatg
     1141 accatgcgac cgttcaattc ggcaggattt gggtaagacg cggcgatgcc ggagaagatg
     1201 atgcgttttt caaaaccgaa atcgaggtcg aatttcaaaa gtttggtgct gccttcgacg
     1261 gcttcgcagt tcaatacttt ggcgacgcgc atatcgattt tcataaagtc gtcgaagctt
     1321 gcctgttcgg cgactttttc gtatttaccc tcttcaacgg gcgcggatgc cgtctgaatg
     1381 ctttgtttgt tggcttcgat caaatcgtcc acttgttttt gctccactcg ttgcattaaa
     1441 tgttcgtatt tgttgatggc gtgttcgcct agggtttcgc gcgtgttctt ccaggtaatc
     1501 gcgtccagat tgaggaaacg cgcggcgttg gcggcggttt gcggcaacac gggggcgagg
     1561 taggcggtca gcatggtgaa ggcgttgatg agttcgctgc atacttcgtg caggcgttcg
     1621 tcttgacctt cttgtttggc gagctcccac ggtttgttgg catcgacgta ttcgttgaca
     1681 atgtctgcca atgccatgat gtcgcgcagg gcgcgggcgt attcgcggtt ttcgtattgc
     1741 tcggcgatgg tgtcgctttc ggcggcgagt tttgccagca atgcgctgcc tgaaacgtct
     1801 ttcggacgac cttcaaagcg tttggcgatg aaacccgaag cgcgggcggc gatgttgacg
     1861 tatttgccga caaggtcgct gttgacgcgc gaaataaagt cttgcaggtt caaatcgatg
     1921 tcttcgattt tgctgttgag cttggcggcg atgtagtagc gcatccactc ggggttcagg
     1981 ccttgttcca aataggattt ggcggtgatg aaggtgccgc gcgatttgga cattttttgt
     2041 ccgtccacgg tcaaaaagcc gtgtgcgtac acgccggtcg gggcgcggtg gccggagaaa
     2101 tgcagcatgg ccggccagaa cagggcgtgg aaatagagga tgtctttgcc gatgaagtgg
     2161 tacatctcgg tttggctgcc ggctttgaag tattcgtcaa aatcgatgcc gatgcggtcg
     2221 cacaggtttt taaacgatgc catgtagccg acgggcgcgt cgagccagac gtagaagtat
     2281 ttgcccggcg cgtcggggat ttcaaaaccg aaatacggcg cgtcgcggga aatgtcccag
     2341 tcggacaggg tggtttctcc gccttcgccc agccattcct tcattttgtt gagggcttcg
     2401 ggttgcagat ggggcttgcc gtcgtgcggg ttgttgccgg aagtccatgc tttgaggaag
     2461 tcggcgcatt cgcccagttt gaagaagaag tgttcggatt cgcgcaattc gggtttcgcg
     2521 ccggaaacgg cggaatacgg gttgaacagt tcggtcgggg aataggtcgt gccgcagact
     2581 tcgcagttgt cgccgtattg gtcttgggcg tggcatttcg ggcattcgcc tttgacgaag
     2641 cggtcgggca ggaacatttg tttttcgggg tcgaaaagct gctcgatgac gcggctttcg
     2701 attttgccgt tggctttcag cgcgcggtaa atgtcttggg aaaactgttt gttttcagtg
     2761 gaatgggtgc tgtaataatt gtcgtagccg atgaaaaagc cggtaaagtc ggcaaggtgc
     2821 tctttgcgca ctttggcaat catgtcttcg ggcgcgatgc cttgtttttg cgcggcaagc
     2881 ataacgggcg tgccgtgggt gtcgtcggcg cagcagtagt agcattcgtg gccgcgcagt
     2941 ttttgaaagc gcacccaaac gtcggtttgg atgtgttcga ccatgtggcc gaggtggatg
     3001 ctgccgttgg catagggcag ggcggaggta actaagattt tgcgtgtcat attgtgcttt
     3061 gcaaacaatg ggtaaaggcg gattataccg caaatcaaac ggggaaatgc cgtctgaagc
     3121 ctgaaaaatc gggcttcaga cggcattttt gccaaccggc gggatttatt cgacggttac
     3181 ggatttcgcc aggttgcgcg gcttgtccac atccgtgccg cgcgcgaggg cggtgtggta
     3241 ggcgaggagc tgcacgggga cggtatgcac gacgggggac agttcgccga cgtggcgagg
     3301 tgcgcggata acgtgcacgc cttcggcggc attaaaattg ctgtcgaggt cggcaaagac
     3361 gaaaagttcg ccgccgcgcg cgccgacttc ctgcatattg gctttgactt tgtccaacag
     3421 gctgtcgttg ggcgcgatga cgacgacggg catgttttcg tccaccaagg caagcggccc
     3481 gtgtttcagt tcgccggcag gataggcttc ggcgtggatg taggtgattt cctttagctt
     3541 caacgcacct tcaagggcaa tcgggtaatg gatgccgcgc cccaaaaaca gcgcgctggt
     3601 tttcttggca aactgttgcg cccaagcggc aatttgcggt tcgaggttca aagcgtgctg
     3661 cacgctgccg ggaagctggc ggagttcttc ggtgtaacgt gcttcatctt cgccggatac
     3721 cacgccgcgc actttcgcca gcgttaccgc caaaccgaac agcacgacca gttgcgtggt
     3781 aaatgcttta gtcgaggcga cgccgatttc cgcaccggca cgggtataaa gcacaaggct
     3841 gctttcgcgc ggcagggcgg attccatcac gttgcagacg gagaggctgt ggcggtgtcc
     3901 caaagatttg gcgtatttca aagcctccat cgtgtccagc gtttcgccgg attgggaaat
     3961 ggtaatgacc agttggtcgg ggtcggcaat cacgctgcgg taccggtatt cgcttgcgat
     4021 ttcgacgtcg gccgggattt tggcgatgga ttccaaccaa tatttggcgg tcagcgcggc
     4081 gtaataggac gtgccgcagg caaggatttt gacgctgcgg atgctttcaa acacgctttt
     4141 ggcgtttttg ccgaagtttt cggggatgaa tccgccgtcg aggaaaactt ccgccgtgtc
     4201 cgcaatcgcg cgcggctgct cgtggatttc tttttgcata aagtggctgt gcggacccgg
     4261 ttccaaagag gcgggcgaga gttcggatac cttgactttg cgttcggcag gcaggccgtt
     4321 tttatcggtc agccgtttga tgccgtctga agccagcagc gcgatgtcgc cgtcttcgag
     4381 gtacgccacg cggcgcgtaa aggcgatgac ggcggatacg tccgaagcga taaaggtttc
     4441 atcgtcgccc aaagcgacca aaagcgggca gcccatacgc gccacaacta attcatcagg
     4501 cttgtcttgg gcaataaccg cgatggcgta tgcgccgtgg aaacgtttga ccgcttcctg
     4561 cacggcttca aacaatttgc cgccgttttg cgcgtattcg tgattgatgc tgtgtgcgat
     4621 gacttcggta tccgtttgcg attcaaaccg gtatcccaaa ccttccaagc gtttgcgttc
     4681 gctttcaaag ttttcgatga tgccgttgtg gacgacggca atcataccgc cgctgatgtg
     4741 cgggtgggcg ttcggctcgg taacgccgcc gtgtgtcgcc cagcgcgtat gcccgatgcc
     4801 gatgccgccg ctgatgcctt tttcgcgtgc cgcgtcctcc ataagctgca cgcgtccgac
     4861 gcggcgcacg cgcttgattt tgccgtctga atacacagca atgcctgatg agtcataacc
     4921 ccggtattcg aggcgtttga ggccgtcggt cagaaaatcg acgacgttgt gatgggcgcg
     4981 gatggcgccg acgataccgc acataaactg ttccttagta tccggttgaa aaaaaagatg
     5041 cggacggctt ccgtaccgca ccttccgttt cggattataa accgcctccc gcgccggaaa
     5101 acagcaaaat gccgtctgaa ggcttgggct tgctcaaaaa agggagggat ttccccgcct
     5161 gaccgggatg ggcgttcaga cggcattgcc tgccgccggt ttatagtttt tgcaaatcaa
     5221 tattaacaag ctgaaaaaaa acaatatacc cgcccggtct taatgttaac ggagtatgga
     5281 aatgaaacaa atgcttttgg ccgtcggcgt ggcggcggtg ttggcgggct gcggcaagga
     5341 tgccggcggt tacgagggtt attggcgcga aaagtcggac aaaaaagagg gcgtgattgc
     5401 cgtcaaaaaa gaaaaaggca attacttcct taataaaatc aacgtgttta caggcaagga
     5461 ggagtctttg cttttgtctg aaaaagacgg cgcgctttcg ataaacacgg ggatagggga
     5521 aatcccgatc aaactttccg acgacgggaa agagctgtat gtcgaacgca ggcggtatgt
     5581 gaaaaccgat gcggcgatga aggacaaaat catcgcccac cagaaaaagt gcggacaaac
     5641 ggcacaggca tacctcgacg cgcgaaatgc gttgccgtca aaccaaacgt atcagcagcg
     5701 tcaggcggcg atcgagcaat tgaaacggcg gtttgaagcc gagtttgacg aattggaaaa
     5761 agaaatcaaa tgcaacggca gaaccccggc attgttgctt tggcagggga caaccgggag
     5821 gatgccgccg tccgaatcgg acgcgtggtt tccataccgg tacgggcggg cgggaatgtc
     5881 cgcctttttt tgttcggatg cgtttgaata cccgtccgat tctgtccgtt tgcaaggggg
     5941 atttccgttc gggcggaaat ccattccgtc aaataagaaa ggcgttttgt ccggccggcc
     6001 ggcttgtgcc tgcggtatcg caaaacttcg ccttaatgcg cccgattggc tagggacggg
     6061 cttcggacgg cattgttttg ccggttcacg ggcggtattc gggcttcatg ccgttgggca
     6121 ggagctgcca gacgtatccc gtggttttct gtttgccggc aagttcgccg gcttcgtcgc
     6181 cgtaacccca aaaataatcc acgcgcaccg cgcctttgat cgcgctgcct gtatcctgcg
     6241 ccataatcag gcggttgagg actttgcggg taaccggatg ggcggtggcg acaaataagg
     6301 gcgcgcccaa ggtaatgtag tgccggtcga ttgcgccggc gtattccccc atcagcggcg
     6361 tgcccagtgc gccgacgggg ccctcattgc cgcttccggc aagctcgcgg aaaaagatat
     6421 agctggggtt ttgccccaaa acttcggcga ggcgttgcgg attttgccgc atataggctt
     6481 tgatgccctg catggaggtt tgcccgagct tgaggtagcc tttgtccgcc atatagcgtc
     6541 cgatggaaac atacggatgc tcgtttttgt cggcatagcc gatgcggatg tatttgccgg
     6601 acggggtttt cagacggccc gagccttgta tgtgcatgaa aaaaagttcg acggggtctt
     6661 cggcgtaacc gagtatcggg gctttgccgt caagcgcgcc gccgttgatt tggttgcgcg
     6721 tgtggtaggg gaggaagcgg cttccttcaa acctgccctt gattgccgtt gtgcgcgcgg
     6781 taatggggaa tcgggaaagg tcggcggtat gcgtgccgcc ggcattgtcg attgtgccgc
     6841 tgttttttcc cgtctgcctg atgcggacaa gggctttccc gccccgcaaa ccggcaggca
     6901 gggggacgga gataaaatcg tcgggaatgc cgtaaatcgg gaagcgggcc cgttccgtcc
     6961 gcctgtcgtc gcccttcagc accggctcgt aatagccggt aaccgtacct gcaaggcttc
     7021 cgttgcctgc aacctgccac ggcgtgaaat agcgttcaaa aaaccgcttt gcctgaaagg
     7081 aatgcacggg ggtttgaaag gcttgggcgc acacatcctg ccagccttgg cggtttttca
     7141 aattggcgca gccgaggcgg aaggattgca ggcttttggc aaaatcctgc gccgcccagt
     7201 ggggcatgga caggtgcggc acaacggtat agacggcccc gccgccggca accgtcgttc
     7261 cggcggggtc ggggatgccg gccggccggt ccgggccgtt gatgacggat gtgtcgggtt
     7321 gcggaaaggt ttggatgctc ctgctttggc aggcggcgag gatggcggcg gcgatgccgt
     7381 acagggcgga gcggagcagg tgttttttca taatggacaa tgttgtcgtc agtaataaaa
     7441 aaagatggtt tcgggcggcg ttgcggtagc cggggagagg ggattttaac acagggcgca
     7501 gccgcagcct gcggaacttt ccgccacgcg gcaatgcaga taaaaataac ttgcatttgt
     7561 atttgcaagt aatgaaaata ttccgataat atattattaa ttatccttgt tcattcgcgt
     7621 ttacgctggt cgctttttta attatgttgc gcgagggtat cgaagccgcg ctcatcgtcg
     7681 gcatcgttgc cggttttttg aaacagtccg gacattcaaa aactgatgcc taaggtctgg
     7741 ttcggggtcg tccttgcttc tttgatgtgt ttgggcatcg gatacggcat ccattcggca
     7801 acgggcgaga ttccccggaa gcagcaggag ttcgtcgtcg gcattatcgg tttggttgcc
     7861 gtcgcgatgc tgacttgtgt ggttttatgg atgaaaaagg cggcgcgttc gatgaagcgc
     7921 cagcttcggg attctgtaca ggcggctttg aaccgtggca gcggtcaagg atgggccttg
     7981 gtcggtatgg cgtttcttgc cgtggcgcgc gaaggtttgg agagcgtttt ttttcctgct
     8041 tgccgtattc aaacagagcc cgacgtggca gatgccggcc ggcgcggtag cgggggtttt
     8101 ggctgccgcc gtgattggcg cgttgattta tcagggcggg atgcgcctga atctggcgaa
     8161 gtttttccgt tggacggggg cgtttctgat tatcgttgcc gccggtctgc ttgccggctc
     8221 gctgcgtgcg ctgcatgagg caggcatttg gaacgcgctt caggacattg tgttcgaccc
     8281 atcaaaatat ttgcacgaag acagtccgtt gggtgtgctg ctcggcggat ttttcggcta
     8341 taccgaccat ccggcgcagg gcggggcctt ggtttggctg ctgtacctta ttcccgtcat
     8401 gatttggttt ttgcgcggca gcaggccgtc tgaaacttta acccgtaaag aggaattgaa
     8461 atgagaaaat tcaatttgac cgcattgtcc gtgatgcttg ccttgggttt gaccgcgtgc
     8521 cagccgccgg aggcggagaa agccgcgccg gccgcgtccg gtgagaccca atccgccaac
     8581 gaaggcggtt cggtcggtat cgccgtcaac gacaatgcct gcgaaccgat gaatctgacc
     8641 gtgccgagcg gacaggttgt gttcaatatt aaaaacaaca gcggccgcaa gctcgaatgg
     8701 gaaatcctga agggcgtgat ggtggtggac gaacgcgaaa atatcgcccc ggggctttcc
     8761 gacaaaatga ccgtaaccct gctgccgggc gaatacgaaa tgacctgcgg ccttttgacc
     8821 aatccgcgcg gcaagctggt ggtagccgac agcggcttta aagacaccgc caacgaagcg
     8881 gatttggaaa aactgcccca accgctcgcc gactataaag cctacgttca aggcgaggtt
     8941 aaagagctgg cggcgaaaac caaaaccttt accgaagccg tcaaagcagg cgacattgaa
     9001 aaggcgaaat ccctgtttgc cgacacccgc gtccattacg aacgcatcga accgattgcc
     9061 gagcttttca gcgaactcga ccccgtcatc gatgcgtgtg aagacgactt caaagacggt
     9121 gcgaaagatg ccgggtttac cggcttccac cgtatcgaac acgccctttg ggtggaaaaa
     9181 gacgtatccg gcgtgaagga aaccgcggcc aaactgatga ccgatgtcga agccctgcaa
     9241 aaagaaatcg acgcattggc gttccctccg ggcaaagtgg tcggcggcgc gtccgaactg
     9301 attgaagaag cggcgggcag taaaatcagc ggcgaagaag accgttacag ccacaccgat
     9361 ttgagcgact tccaagctaa tgcggacgga tctaaaaaaa tcgtcgattt gttccgtccg
     9421 ttgattgagg ccaaaaacaa agccttgttg gaaaaaaccg ataccaactt caaacaggtc
     9481 aacgaaattc tggcgaaata ccgcaccaaa gacggttttg aaacctacga caagctgagc
     9541 gaagccgacc gcaaagcatt acaggctcct attaacgcgc ttgccgaaga ccttgcccaa
     9601 cttcgcggca tactcggctt gaaataagcc gcaagcgttc agacggcatt tggcggcaga
     9661 taccgtctga
//
LOCUS       DAXDXW010000082         9292 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXDXW010000082 DAXDXW010000000
VERSION     DAXDXW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.083"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            293..1147
                     /locus_tag="ACFB64_001809"
     CDS             293..1147
                     /locus_tag="ACFB64_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
                     /protein_id="HFC1210115.1"
                     /translation="MSNGARWTVTNDSMLKELDLSEDAQVEFSDNNKFVKVSVSKLKG
                     DGGVFKMYGDIVKGESDKLITRKGSEGTHIIEYMDDAKAKTTGREYLKLVENKGNQED
                     NKASNKASYKLNVRCTEQGGWCFALGESGASKKVNISTDGKRDFYLYPDTLTPGASSS
                     VLFGEALYQLNAVSDETLVQRMGEIHADGTPQEDNNVWIKRVGGKFSGSRSDYRVGGY
                     GNRYWGFAGGFNRTGFGDKWIHYKGLMLRHLQSSYASEDYVGSGKILRQGGRCLFRLA
                     QPGKQGLL"
     gene            1237..1695
                     /locus_tag="ACFB64_001810"
     CDS             1237..1695
                     /locus_tag="ACFB64_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC1210116.1"
                     /translation="MPICFPPKPVGGWKNKTAAKTYRWQPEVQLSYWFTRGYGFPLSN
                     GLSAETDNFRSLMGRFGFRAGVDGLDGGRLNIYGKLMYKREFIGTIRHRFNGSAVEEF
                     KHRGGWLEYGLGVVRRNAGNGRQLYFEAQRSSMHTMRQNWQVNMGVRSMF"
     gene            complement(2178..3590)
                     /gene="trkA"
                     /locus_tag="ACFB64_001811"
     CDS             complement(2178..3590)
                     /gene="trkA"
                     /locus_tag="ACFB64_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC1210117.1"
                     /translation="MKILILGNGQVGSTVAQNLAAITNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(3688..5211)
                     /locus_tag="ACFB64_001812"
                     /pseudo
     CDS             complement(3688..5211)
                     /locus_tag="ACFB64_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
     gene            complement(5518..6324)
                     /locus_tag="ACFB64_001813"
     CDS             complement(5518..6324)
                     /locus_tag="ACFB64_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC1210118.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPGKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKKMNKVGVVTGHTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEGGQAAK"
     gene            complement(6376..6627)
                     /locus_tag="ACFB64_001814"
     CDS             complement(6376..6627)
                     /locus_tag="ACFB64_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210119.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            6729..7499
                     /locus_tag="ACFB64_001815"
     CDS             6729..7499
                     /locus_tag="ACFB64_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210120.1"
                     /translation="MDTNNQDKEPNWEKRLENLRQTRRMNTAPAYVPLTKPSEVGKEE
                     PIPKLDDANRDRVLKAYLKKWQEEHNSAVADCGEEVETDPMIVLQENWLNAQASLQMP
                     VSEKHIESRRRIRFDAKKDSAEFETAQIEGGENADAAESGTAADGVAENGEGGAEEAL
                     MPVQINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGMIRTAVQKQMALFT
                     YQLQQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            7645..8814
                     /gene="metZ"
                     /locus_tag="ACFB64_001816"
     CDS             7645..8814
                     /gene="metZ"
                     /locus_tag="ACFB64_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC1210121.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTIAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGGH
                     VVSSRSLFGTTVGFINNIVTKFGIGVSRVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALRIAQWLQE
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITNPWTTTHGRMQPEEKLAAGIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            8909..9115
                     /locus_tag="ACFB64_001817"
     CDS             8909..9115
                     /locus_tag="ACFB64_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC1210122.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAVLF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT         2129 a         2280 c         2712 g         2171 t
ORIGIN      
        1 aatgttaagc agaaacgatt gtctcaaaaa aagcaacttt ggataaatac aatcaagaat
       61 tggctgaact aggtgaaaat gaaaagaaaa aagaagaact agaaaaaaag taaaacagca
      121 agaaaacaaa attcagatca gaaaaaggca catagaacgt ctaaaagaag acatagaaaa
      181 ggttgcggta ctcgaagaag aaatcaaaag atctgaaaag ttcttcgatg aaaacggttg
      241 gcttaaagac agcattatcg acgacaaaac aaaaaacgcc gtcaacctca aaatgagcaa
      301 cggcgcgcgt tggacggtta ccaatgattc aatgctgaaa gagttggatt tgtcggaaga
      361 cgcgcaggtc gaatttagcg acaacaataa attcgtcaaa gtgtccgtga gcaagctcaa
      421 gggcgatggc ggcgtgttca aaatgtatgg cgacatcgtc aaaggagaat cggacaagct
      481 gattacccga aaaggcagcg aagggacgca catcatcgaa tatatggacg atgccaaggc
      541 aaaaacgacg ggaagggaat atctgaaact ggtcgaaaac aagggcaatc aagaagacaa
      601 taaagcatca aataaagcat catacaaatt gaatgtccgc tgtacggaac agggagggtg
      661 gtgttttgcc ttgggtgagt cgggtgcttc caaaaaggtc aatatttcca cagacggtaa
      721 gcgggacttc tacctctacc cggataccct gacgccgggc gcatcgagca gcgtcctttt
      781 cggcgaggcg ttgtatcagt tgaacgcggt ttccgatgaa acgctggtgc agcgtatggg
      841 cgaaatccac gccgacggaa cgccgcagga agacaacaac gtttggatca aacgcgtcgg
      901 cggaaaattc tccggcagcc gcagcgatta ccgcgtgggc ggttacggca accgctattg
      961 gggctttgcc ggcggcttca acaggacggg gttcggcgat aaatggattc attacaaagg
     1021 gctgatgctc cgccacctcc aatcgtccta cgcttccgaa gactacgtcg gcagcggcaa
     1081 aatcttacgg cagggcggca ggtgtttatt ccgcctggct caaccgggaa agcagggctt
     1141 attatgattt ggtcgccaac atcgcccgat ataagggcag ctacgggctg accaattacg
     1201 ccggcaaacg ggtcgaatcg gacgaggcgc gcctgaatgc ctatatgctt tccgccgaag
     1261 ccggtaggcg gatggaaaaa caagacggcg gcaaaaacct atcggtggca gccggaagtg
     1321 cagttgtctt actggtttac gcgcggctac ggctttccgc tgtcaaacgg gctgtctgcc
     1381 gagacggaca acttccgcag cctgatgggg cggttcggct ttcgggcggg cgtggacggt
     1441 ttggacggcg gcaggctgaa catctacggc aagctgatgt acaaacgcga gtttatcggc
     1501 acgatccgcc acaggttcaa cggctctgcc gtggaggaat tcaaacaccg gggcggctgg
     1561 ttggaatacg gcttgggcgt ggtgcgccgc aatgccggaa acggacggca gctttatttt
     1621 gaggcgcaaa ggtcttcgat gcacacgatg cgccagaatt ggcaggtcaa tatgggcgtg
     1681 cgcagtatgt tctgatgcgc ggatacgcct gaaaacaatc cggaacggtc agacgcgttc
     1741 cggattgttt ttttgtcggg gcggtttgct gccatatcaa aacaggcggg cgatgccgtc
     1801 tgaaacttcg tcattcccgc gaaagcggga atctagaacc tcaaactttc agataatctt
     1861 tgaatattgc tgttgctcaa tggtttggat tcccgcaacc tttcgtcatt cccgtgaaaa
     1921 cgggaatcta gaatctctaa agcttcagct aacctttgaa tattgccgct ttccaatggt
     1981 ctagattccc gcctgcacgg gaatgacgat ttggaaatta cccgaaaccc aaaaacaact
     2041 gaaaccgaac aggccggatt cccgctacgc gggaatgacg gggggcggat gccgtctgaa
     2101 gcggtgggct gaagcccact ctactattat ttttgcttcc ataccgcttg gcggcaatgt
     2161 gtccggaatg gagcggttta tccgaaaaag cccattttga cttggatgag tttctccagt
     2221 tcgttcagga tgcgccggcg cgagacgaaa aagatgatgt ggtcaccgtc ttggatgacg
     2281 gtttcggtat ggtgtcccat aatggtttcg ccggttccgg cgcggacgac ggcggcaatg
     2341 tggcagcctt cgggccattt gatgccgctg atgcgcctgc cgatgatggc ggaagttttt
     2401 ttgtcgccgt gcgcgacgac ttcgatggct tccgccgtgc cgcgccggat ggggtggacg
     2461 gcaacgatgt cgccgcgccg gatgtgggcg agtatcgagc cgatggtgat gaggtggggg
     2521 gagacgacga tgtcgatttt gttgccttcg agcaaatcga cgtagcttga gcggttgacg
     2581 atgccgatga cgcgcttcgc gccgaggttt ttcgccaaaa gggcggacat aatgttgctt
     2641 tcgtcgtcgt tggtcagggc gcagaatacg tcgatttcgt cgatgtattc gttgtcgagc
     2701 agggtttcgt cggttgccga accttgcagg acgagggtgt tgtcgaggtt ttcggctatc
     2761 cattcggcac ggcgcggccg gcattcgatg attttgacgt tgtatgcgtg ttcgagctgc
     2821 ttggcgaggc ggtagccgat gttgccgccg ccggcaatca tgatgcggcg ggtgctggtt
     2881 tctttggggc gcaattcggg tatgaccgcc ccgatgtttt cggcggcggc ggcaaacagg
     2941 atttcgtcgc cttcgatgat gacggtttgc ggcgcgggga caatgaggcg gttgttgcgg
     3001 taaacggcgc agatttggca gtcggccccg tcgggcaaat cttgggcgat gtcggcaatg
     3061 ctgcgtccga caagcagccc gccgcgccgc gcctgtatga tgaccatccg cacgcggtcg
     3121 tctgcaaaac gtaaaacctg caatgcgccc gggcagtcta tcaggccggc aagctgttcg
     3181 gtaacgagct gttcggggct gatggtttcg gttatgccga atatggaaag gctgccgttt
     3241 tcgttgtttt cgagcttggg gctgaggtat tcgaggtatt cgctggaacg gacgcgcgcg
     3301 atgcggccgg ggatgttgaa caggtcggcg gcaactttgc aggcgacgat gttggtttcg
     3361 tcgctgcggg agagcgcgag cagcaagtcg gcatcttccg cgccggcgcg ttctaatgtg
     3421 aagggggatg cgccgttgcc gaaaacggtt tggacatcga ggcggctgcc tgtttcctgc
     3481 aatgcttttt cgtcgatgtc gataacggtt acgtcgttgt tggtgatggc ggcaaggttt
     3541 tgcgcgacgg tagaacctac ctgcccgttg cctaaaatga ggattttcac ggtatgggtc
     3601 gccgggtgat gaaatggtgg attgtaccgc aaccggcggc ggaaatgccg tctgaaggca
     3661 gattttgcgt tcagacggca ttgcgcttca agactcgacg gggatgatgc cgattttcgc
     3721 ctgccatttg cgcggggcgg tggcgtggat ggattcgcct ttgctgtcca cggcgacggt
     3781 tacgggcata tctttgactt caaattcgta aacggcttcc atacccaatt cggggaacgc
     3841 caagactttg gaagatttga tggcttttgc cacgaggtat gccgcgccgc cgactgccat
     3901 gaggtacacg gccttgttgt cggcgatggc ttcgcaggtg gccgcgccgc gctcggattt
     3961 gccgatcatg cccaagaggc cggtttgttt gagcatttgg cgggtaaatt tgtccatgcg
     4021 ggtggctgtg gtcggacctg cgggaccgac gacttcatcg ccgaccggat cgacggggcc
     4081 gacgtagtaa atcaggcggt tggtgaaatc gacgggcaac tcctcgcctt tgtcgagcat
     4141 attgacgagg cgtttgtgcg cggcatcgcg gccggtgagg attttgccgt tcaacagcaa
     4201 tacgtcgccg gttttccagc ttgccacttc ttctttggtc agcttatcga catcgacgcg
     4261 tttgccgttg tcggggctgt aagtcagatc gggtcagtct tcgacgcgcg gcggcgtgag
     4321 ttcgacagga cctgagccgt ccaattcaaa ttcgacgtgg cgggtggcgg cacagttggg
     4381 aatcatggca atcggtttgg aggcggcatg ggtcgggtaa tcgaggattt tcacgtccaa
     4441 cacggtggtc agaccgccca agccttgcgc gccgatgccc agcgcgttga ccttttcaaa
     4501 gagttcgagg cgcagggctt cggtggtgga caattccgcg ccggacgcgg ctttttcctg
     4561 caattcttgg atgtcgatgt ggctcatcag ggattctttc gccatcaaca cggctttttc
     4621 gggcgtgccg ccgatgccga tgcccaagat gccgggagga caccagcccg cgcccatcgt
     4681 cgggatggtt ttcaataccc aatcgacgat gttgtcggaa gggttgagca tagcgagttt
     4741 ggatttgttt tcagagccgc cgccttttgc cgcgcaggtt acttcgactt taccgcccgg
     4801 cacgatgctc atgtggatga cggcgggggt gttgtctttg gtgttttggc gtttgccggc
     4861 cggatcggcg aggacggaag cgcgcagggt gttgccttcc caagtgtagg cgcggcgtac
     4921 gccttcgtta accatctctt ccacgctcat gtccgcatcc cattgcacat ccataccgac
     4981 tttgaggaag acggttgcga tacctgtgtc ttggcagatg gggcggttgt tttcggcaca
     5041 catacggctg ttgaccaaaa tctgcgtcat cgcgtctttg gcggcgggat tttcttcctt
     5101 ctgccacgcc ttataaagcg cgtcgatgta gtcttttgga tggtagtagc tgatgaattg
     5161 gaaggcatcg cagatacttt gaataaagtc ttcttgcttg ataacggtca tgattgtgtt
     5221 ccttttcaag gttgtggcgg taagtgtctg ctgctgtttt aaacaacctt tttgaaggct
     5281 gttttttatc aggttgaaat agtgccagcg tttgtacggt gaagacaata cataattcta
     5341 atctcttgat tatcaatttg ttttaataaa atactgtttt attcggattt tgatatctgt
     5401 aatctttgcc tgttgctttg cggtagggag aaatcagcag gcagttcatt gtggaccggt
     5461 tgatgaacaa tgccgtctga aagggcttca gacggcattt tgttcggacg ggcgggctta
     5521 tttcgcagcc tgtccgccct ctttggcaaa atatttggcg tagatcttgt cgtattcgcc
     5581 gctttcgcgt actttttcca acgcatcgtt cagcattttg acggttgctt cgtcgccttt
     5641 gcgtaccgcg atgccgtagt gttcggtggt gaagtcgggc agggtaacga agtccattcc
     5701 tttggccggg ttgtttttca cataattggc gatgaccgcg ctgtcgctga ccacggaatc
     5761 caagccgccg ttttccagtt ctttgataat cagggggacg ttttcgaagc gcgcgatttt
     5821 cggattgtcg ttgcccaaga gtttggaaac ggagaaatcg cccgtgtggc cggtaaccac
     5881 gccgactttg ttcatctttt tcaaatcttc ggaagaagat acttttttgc ctttcggaac
     5941 gaggacgact tgggtgattt caaaatacgg gtcgctgaaa tccatagact gtttgcggtc
     6001 gtcggtaatg gttacgcccg acatcacaac gtccgcatcg ccgttgttca aggcggggaa
     6061 aaggctgtcc cacggctggt gtttgaattc gattttaaaa ttgcccgcct tcgccatcgc
     6121 gttcatcaaa tccacgtcga aaccttcgac attgcctttc gagtctaaag attcaaaggg
     6181 ggcaaactcg gcgttggaag ccacgcggta cactttgccg gggttggcgg caggcgcggc
     6241 ggcatctttg ccctgaccgc cgcaggcaga cagcgcgagt gcggaacagg caagggcggc
     6301 ggcaatccat tttttcatat tcatcaaaat tcctttcggt aatcaaaaag cctaaaaaat
     6361 cagtttaaca taaaattaac gttccttttc acggaaaaac gcataagtaa cgtcgtggaa
     6421 caaatccgta tgcgggctgt cctcatagct caattctgcc aaggcgtcct caaaagcaag
     6481 ctgaatcgac tcgaccgcga tttcggcggt gtctgcggga agggcgcgga tcagggcttt
     6541 gaaggcggtg gacaggacgt ggttctgcat acgcagcaca tcgtttgctt cttccagata
     6601 ttccaaacgc tgttcgatgc tgcccatgat gtatcctcgc gaatctgtta aaatgcgcca
     6661 ttatatcaaa ctgcgctttg ggcgcgggtt tgcccgcctg caaagattaa gaaccggaag
     6721 ccgaaaaaat ggatacaaac aatcaggata aagagccgaa ttgggaaaaa cgcctggaaa
     6781 acctgcgtca gacgcgccgt atgaataccg cgcctgccta tgtgccgctg accaaacctt
     6841 ctgaagtcgg aaaggaagag cccataccga aattggacga tgcaaaccga gaccgtgtgt
     6901 taaaggcgta tctgaagaaa tggcaggaag aacacaacag cgccgttgcc gattgcggcg
     6961 aggaagtcga aaccgacccg atgatcgtcc ttcaggaaaa ctggctcaac gcgcaggctt
     7021 ccctgcaaat gccggtttcg gaaaaacaca tcgaatcgcg gcggagaatc cggttcgatg
     7081 ccaaaaaaga cagtgccgaa tttgaaaccg cacagattga gggcggggaa aacgccgatg
     7141 cggcagaatc cggtacggcg gcagacgggg ttgcggaaaa cggcgaaggc ggtgcggaag
     7201 aggcgttgat gcccgtccaa atcaatatcc tgaacccgaa agccgtcaac cggcgcgaag
     7261 tgttctgcct gtccgaacag gagctgaccg aacggttgat caaacgcctg cgcccgcatc
     7321 tgaccgatac cgtcaacggc atgatccgta cggcggtgca gaagcagatg gcgctcttta
     7381 cttatcagct ccagcaaatg ctgcacgagc aggcgggtgc ggtagtcgaa gacgtgttgg
     7441 aacacgatgt ccgtaaaatc ctcaacgaca tcaagtacga attaaaatac aaacgctgat
     7501 tgccatgccg tctgaaagcc gtgttcagac ggcattttcc tgcccgtccg acttgaaagc
     7561 ctcatggaac aggcgcaaaa tattaataaa ttaaacaacg ggtgcacgcc ccgttgccga
     7621 acggacaaaa ccgaggaatc accgatgagc aaaaaacttc atccgcaaac gctcgccata
     7681 cgcggcggca aagaacaaac cggataccgc gaacacaatc aggcattgtt cctgaccagc
     7741 agcttcatgt gggacaacgc ccaacacgct gccgatttgt tttcaaaaaa aatcaaaggc
     7801 ttcacttata cccgtaccgc caacccgacc atcgcggcct ttgaaaaacg catagccgct
     7861 ttggaaggcg cggaacgcgc ggtcgccact tcgaccggca tgtccgcgat tcaggcggcg
     7921 tttttcacct tcctgcaggc gggcggccat gtggtttcca gccgcagcct gttcggcacg
     7981 accgtcggct ttatcaataa catcgttacc aaattcggca tcggagtgag ccgcgtgtcg
     8041 ccgactgata taaatgaatg gaaagccgcc gtcaaagcca acaccaaact gctgtttttg
     8101 gaaacgccgt ccaacccctt gggcgaagtg gccgacttgg aagccttggc ggaattggcg
     8161 cacggcatcg gcgcgctctt ggtggtggac aacagcctgc tgtcgcccgt cggctcgcag
     8221 cctttgaaac acggcgcgga tatttccgtt tcctccgcca ccaaagccat agacggacac
     8281 gggcgcgtga tgggcggcgt gttggcgggt tcggaagaac tgatggcgca ggtcgccgtt
     8341 tactgcaact cttgcggttt ggcgatgtcg ccgtttaacg cgtggcagtt gttgagcggc
     8401 gtggaaaccc tgtcgctgcg tatggaaaaa cagtttgaca acgccttgag aatcgcgcaa
     8461 tggctgcaag agcagccgca ggttcaagca gtgtactaca ccggattgcc cgaccatccg
     8521 caggcggagc ttatccgcaa acagcaaaac ggcggcggca tcgtcatcgg ttttgaagtc
     8581 gccgaccaag cagccgcgtg gaaagtcgtg gacggcgtgg aactcttttc ccgtaccgcc
     8641 aacctcggcg acgtgcgctc gaccatcacc aatccgtgga caaccacgca cggcaggatg
     8701 cagcccgaag aaaaactcgc cgccggtatc cgccccggac tggtgcgcct gtcggtcggt
     8761 ttggaatatg ttggcgattt gattgacgat ttgaaacagg cactcgcgcg ctgattgcgt
     8821 gtttcagacg gcatcggcgg aaaaatgcgg cagatgccgt ctgaaaccct tattgcgtat
     8881 ggcatacaca tcaacaatag gaaaaaatat ggatgaagcg gtttttgccg attgggcgtt
     8941 gaaaatctgc ctgaccggtt tgattatttt tttgggtttt atcgtttgga atttgggcaa
     9001 ggagtccaaa gcaggcaaat tcggcatcgc cgttttgttt ttggttttgg gcttgggtgt
     9061 gttcggtttt gtgttcaaag agttattgat taagtttttg gtattgccca aataggtttg
     9121 ccgtctgtcg aaatgccgtc tgaaatcata aaaagtaatc tgcaccctaa aagtttgatt
     9181 taaccgccaa ctgattaagg tgcgggtttt tatgacaaaa taaaaatcat ctttccgcaa
     9241 tcattcccac ggatgcgaaa gcctgtcttt ttgaatcccg gataatttta ca
//
LOCUS       DAXDXW010000083         9146 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXDXW010000083 DAXDXW010000000
VERSION     DAXDXW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9146
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.084"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(102..2954)
                     /gene="gcvP"
                     /locus_tag="ACFB64_001818"
     CDS             complement(102..2954)
                     /gene="gcvP"
                     /locus_tag="ACFB64_001818"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC1210123.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTLPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVSDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPKGVKRIANRIHTLASVFADALVSDGLKVVHEVFFDTVTVDFGSK
                     EKADQVFAAALESGYNLRRVNNTQVAAAFHETSVYEDLADLYRAFTGKDTFTFADDVK
                     GRLNAELLRQDDILQHPVYNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWTEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASAGQT
                     SVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRIAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGEWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(2985..3344)
                     /locus_tag="ACFB64_001819"
     CDS             complement(2985..3344)
                     /locus_tag="ACFB64_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210124.1"
                     /translation="MTDRPNLLRIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNVVFPLPAHPLAWLVPAGFMVLGGGFPLSLPLVALLVPTCCILARCPPLS
                     RLLCHPCPNHPMSKNSA"
     gene            3343..3522
                     /locus_tag="ACFB64_001820"
     CDS             3343..3522
                     /locus_tag="ACFB64_001820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFC1210125.1"
                     /translation="MPSEPAGRLSQKTPYRCNFMRLYGVGFALQTAYASRSSLVSQSV
                     HDYINNLGADKPLFI"
     gene            complement(3515..4156)
                     /locus_tag="ACFB64_001821"
     CDS             complement(3515..4156)
                     /locus_tag="ACFB64_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC1210126.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGNNNLKSDQIHANGKGYRKFAENLNQFLRKHGFR"
     gene            complement(4146..4697)
                     /locus_tag="ACFB64_001822"
     CDS             complement(4146..4697)
                     /locus_tag="ACFB64_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC1210127.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIKDDNGLFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFGEADIERLLTDAGIVRNRLKIDAAIF
                     NARQIQALQQEHGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRKTLKYHPKWLDAV"
     gene            complement(4701..5219)
                     /locus_tag="ACFB64_001823"
     CDS             complement(4701..5219)
                     /locus_tag="ACFB64_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC1210128.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRINTAQNHVWADTLDDMLEAALSNAFNRLDSTRTFVPAS
                     RSGSTDKWTVYIDAFQGSYTGKTLISGYAVLPDGKNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5219..6880)
                     /gene="pqiB"
                     /locus_tag="ACFB64_001824"
     CDS             complement(5219..6880)
                     /gene="pqiB"
                     /locus_tag="ACFB64_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC1210129.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSGEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEIANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGMVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGGKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATRGGGLDDLQVKLADLLDKFNNLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLVGNPQTQNIPNELNQTLKELRITLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNNSSKDPIPKGSR"
     gene            complement(6873..8207)
                     /locus_tag="ACFB64_001825"
     CDS             complement(6873..8207)
                     /locus_tag="ACFB64_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HFC1210130.1"
                     /translation="MPPFIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCR
                     TDVPQLDKGEAAFCPRCGHKLFRVGSHPFSGPPAYAAASLILMAFAYSMTYIEVGIPG
                     AASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALR
                     LATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAKVRFGPAFYLMFALSVMLIRTSVSV
                     PQHWVYFQIGRLTGNNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLS
                     ISSAFLTAAVVLYFPANILPIMISSNPAATEANTIFSGIAYMWDEGDRLIAAVIFSAS
                     ILVPVLKIAAMSVLIAAARFALPAGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFH
                     TYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            8518..>9146
                     /locus_tag="ACFB64_001826"
     CDS             8518..>9146
                     /locus_tag="ACFB64_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC1210131.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDPELNKAISELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAVGWLYCAEGSNLGAAFLFKHAQKLDYTGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAQEAFAFYKVILRETFGLPEGTEAPEGMMPHRH"
BASE COUNT         2033 a         2365 c         2614 g         2134 t
ORIGIN      
        1 agttatagtc gagatacgtg tatcggtgca accggtctgc cgttgacgaa aatgccgtct
       61 gaaagtgttt cagacggcat ttgtcttcgg ggtttcaaca gtcagtcttc ataattttcc
      121 atcggcgggc agctgcacac gaggttgcgg tcgccgtaca cgtcgtccac gcggttgacg
      181 aagggccaga acttgtgttc gcgcacgaag ggcagcggga agacggcttc ttcgcgagag
      241 tacggatgcg cccattcgcc ggttatatct gcggcggtgt gcggcgcgtt gaccagtggg
      301 ttgtcgtctt tcggccattc gccgtcgatg actttctgca cttcgcggcg aatggatttc
      361 agggcggcga tgaagcggtc gagttcggct ttgctttcgc tctcggtcgg ttcgatcatc
      421 agcgtgccgg caacagggaa ggaaaccgtc ggcgcgtgga agccgtagtc catcaggcgt
      481 ttggcgatgt cggtttcggt gatgccgctt tcggctttga gcggacgcaa atcgacgatg
      541 cattcgtgcg cgatgcggcc gtttttgcct gtatacagaa tcggatagtc ttcgctcaag
      601 cgtttagcga catagttggc gttgagcaat gcccagcgtg ttgcctgttc catgccttgt
      661 ttgcccatca tggtcaggta catccaagta atcggcagga tggatgcaga accgaaggct
      721 gcggcggcaa cggaggtttg cccggcactt gcgctgtggg tgtcggtcag ggtatgcccc
      781 ggtgcgaacg gggcgaggtg ggctttcaag ccaatcggac ccatgcccgg gccgccgccg
      841 ccgtgaggga tacagaaggt tttgtgcagg ttcatgtgca acacgtccgc gccgacttcg
      901 gcgggctgca tgatgccgat ttgggcattg aggtttgcgc cgtccatgta aacctgtccg
      961 ccgttttcgt gaatgatccg gcagatgtcg cggatgcctt cttcgtacac gccgtgtgtg
     1021 gacggatagg taatcatgat ggcggacaag gcatcgcggt gttgttcggc tttggctttc
     1081 aaatcgtcaa tattgacgtt gccgtgttcg tcggtatcga cgacgacgac tttcaaaccg
     1141 agcatggcgg cggtggcggg gttggtgccg tgggcggatt tgggaatcag gcagatgttg
     1201 cggtgtgctt cgccttgggc ttcctgataa cggcggatgg cgagcatacc gctgtattcg
     1261 ccttgtgcgc cggagttggg ctggaaggaa atcgcgtcaa agccggtgat ggctttcagg
     1321 ctgttttcca tatcagcaag caattcgcgg tagccggcgg tttgcgcttc gggagcgtaa
     1381 gggtggatgt cggagaactc agtccaagta atcggcaaca tttccgcagt cgcattgagc
     1441 ttcatggtgc agctcccgag cgaaatcatg ctgcggttca tagccaagtc gcggtcttcg
     1501 agttttttca ggtagcgcaa catttcgtgt tcggtgtggt aactgttgta cacaggatgt
     1561 tgcagaatgt cgtcttgacg cagtaattcg gcgttcagac ggcctttgac atcatcggca
     1621 aatgtgaacg tatccttgcc ggtaaacgcg cggtacaaat cggccaaatc ttcgtatacc
     1681 gacgtttcat ggaatgcagc cgcaacttga gtattgttga cgcggcgcag gttgtaaccc
     1741 gactccaaag cagcggcaaa cacttggtct gctttctctt tgctgccgaa atcgacggta
     1801 acggtatcga agaagacttc gtgaaccact ttgaggccgt ctgaaaccag cgcgtcggca
     1861 aagacggaag ccagtgtgtg aatgcggttg gcgatgcgtt tcacgccttt ggggccgtga
     1921 taaacggcgt acatgcccgc caagttcgcc agcaatgcct gtgcggtaca aatattggat
     1981 gtcgcttttt cgcggcggat gtgttgttcg cgggtggaca aagccatgcg caaggcaggt
     2041 ttgcccgatg cgtctttgga tacgccgatg atgcggcccg gggcggagcg tttgaactcg
     2101 tctttaaacg cgaaataagc ggcgtgcgga ccaccaaagc ccatcggtac gccgaaacgt
     2161 tgggtgttgc ccaaagcaat atctgcaccc aattcagccg gagacttcag caaaaccaag
     2221 ctcatgatgt cggcggcaac agcaacgatt gtgcctttgg ctttcagacg gccgataacg
     2281 tcctgcaagt cttgcacgtc gccgtcttta ccgacgtatt ggaacagcgc gccgaagtat
     2341 tcgccttcat ccgcttgggc aaaatcgctg accaccagct cgaagccgaa atatttggcg
     2401 cgggttttca tcacgtccaa agtttgcgga tacacgcgcg catccacaaa gaaacgctcg
     2461 gatttcactt tgcccacgcg gtgcgccatc gccatcgctt cggcggcggc ggtcgcttcg
     2521 tccaacaaag acgcgcccgc cacagggaaa ccggtcaaat cgatgcacac ctgctggaag
     2581 ttcaacaacg cttccaaacg accttgggca atctccgcct gatacggcgt gtaggcggtg
     2641 taccagcccg ggttttccaa tacgttgcgc aaaatcacgt tcggcacgcg ggtcgggtaa
     2701 taacccaagc cgatatagga cttgttgatc acgtttttcg acgcgatgcc tttcaatttc
     2761 gccaatgcgt ccgcctcggt cagggcttcg ggcaaatcga gttcggacgg catacggatg
     2821 ctttgcggca aggtgttgcc gacaaagtcg tccatgctct tctcgccgac agcggcaagc
     2881 aacgccgctt cgtcgccgaa actcaaatgg cgcgcggcaa attcgttggg gttgaacagt
     2941 tcggacagtt tcatttctac tccttcgggt ttgtagggct gccatcaggc agaattttta
     3001 gacatcggat gattcgggca agggtggcac aaaaggcggg ataatggcgg acaacgcgcc
     3061 agaatgcagc aggtcgggac aagcagcgcc acaagcggca gcgataaggg aaagccgccg
     3121 cccaaaacca tgaaacctgc aggcaccagc caagccaagg gatgagccgg aagcggaaaa
     3181 acaacattga gcagaaccaa aatcacgaca aacagcggca gccccgccat caggttggca
     3241 cgcctgtatg ttgcgtacgg gtcggatgct tcttcaggtt cgcaatattc catgatttca
     3301 gcctgtccgg gctttatccg cagcaggttc ggacggtcgg tcatgccgtc tgaaccggca
     3361 ggccgtcttt ctcaaaaaac gccataccgc tgcaacttca tgcggctgta tggcgtaggt
     3421 tttgcacttc agacggcata tgcgtcccga agcagtttgg tgtcccagtc ggttcatgat
     3481 tatataaaca acttgggtgc ggataaacct ttgtttatct aaacccatgt tttctcaaaa
     3541 attgattcaa attttcggcg aatttccgat agcctttgcc gttggcgtgg atttggtcgg
     3601 atttcagatt attattgccc aaaatttccg cccacgcgcc gccgaacaac ggaatgccgt
     3661 attcctcgga caaatcctca tacagcggat ggtcgctcaa atgcccgaac aacgcgccca
     3721 gtgtgatgtg cggcacgccg acgaggacgg cgggaatgtt ttccttttgc acggtttcga
     3781 tgattttcgc gatattggcg cgggtctgct cctcgggaac tttgcgcaga aagtcgttgc
     3841 cgcctatgcc gacaatcaca agcttgggtt tgcgtgccaa cagcgcgggc aggcgcgata
     3901 gggcttgcgc ggacgtatcg cccgatacgc cgccgttgac aatattccaa cccgtcagtt
     3961 tttgcagttg cgcggggtag gattcgccgg ggtttgctcc gtagccgaag gtgagcgaat
     4021 cgcccaaggc aagcacggtg cttccttcgg gaattttggc gtgggttcgg gcggattttc
     4081 tgccgcaggc ggtaagaagc aacgcgcctg cgccgaggag gaaggttctt ctgttcattg
     4141 gtttttcaga cggcatcgag ccatttcggg tggtatttca gggttttacg gtaaaccgga
     4201 cagctttcgg cgtgcgcgcc tttgaggtag ccggtactca tcagaaattc gccgacgatt
     4261 tcgccgccga cgaatttgaa atgtttttta aagagtttga cccattcgtc tttgcttcgc
     4321 ggatggtgcg cgtcgagcca gttcttgaac gaaccgtgtt cttgttgcaa cgcttggatt
     4381 tgccgtgcat tgaaaatggc ggcatcgatt ttcagacggt tgcggacaat acccgcatcg
     4441 gtaagcagcc gttcaatatc ggcttcgcca aatgcggcga ccgtatcgat gtcgaaacct
     4501 tcaaatgccg tctgaaacgc ctgccgcttc ttcagcatca gcgtccaact taatcctgcc
     4561 tgattgattt ccaacaccag ccgttcaaac agtccgttgt cgtccttaat cggaaaaccg
     4621 tattgcgtat cgtggtaatg tttgttcggg ttgtcggtgt tttcgggaag tgaggcggca
     4681 aattcgcaat agttcacggt ttactcgacc atctgttgcg ccgcctgttt cagtccctgt
     4741 tcgagtgcgg cggtcatggc ggcgtagccg tcaccctgct gttcggtttc gatatggaag
     4801 ggtctgttct taccgtcggg taggacggcg tagccgctga tgagggtttt gcccgtgtag
     4861 ctgccttgga atgcgtcgat atagaccgtc catttgtcgg tactgccgct gcgtgaggca
     4921 ggaacaaagg tgcgtgtgct gtccaaacgg ttgaatgcat tgctcaacgc cgcttcgagc
     4981 atatcgtcca aggtgtctgc ccaaacatgg ttttgtgcgg tgttgatgcg gtaggggtcg
     5041 gtttgataga ccagtccgcc gcgtttgagc ggttcggcaa gacggacttc gacggcggtt
     5101 tcgccgcctt gcgttgcagg acggatgtag cggctgtcgg gcaacacgaa atattgtgtg
     5161 ctttgcacag taccgcaggc ggcaagcgtc agggcggcgg cgatggggaa aaggcgcatt
     5221 atcggcttcc tttcgggata gggtctttgc tgctgttgtt gaaaatcagt gcgttgggtt
     5281 tttctttcaa agtgttaatg acgggttgaa cgtcttttaa ggttttgtcc aaactttgca
     5341 gcgtattttg tacgtctccg tagataggcg attgaggcga tacgccttgc agggttatgc
     5401 gcaactcttt cagagtttgg ttcagttcgt tcgggatgtt ttgcgtctgc ggattgccga
     5461 ccagtttgtc aatggagctt agggcggcat tggcggattt gagtgcggac ttgagttcgg
     5521 cgagcgagcc gttcaattcg gcaacggttt tatccaatgg cagattgttg aatttgtcca
     5581 gcaaatccgc caatttgacc tgcaagtcgt ccaaaccgcc gccccgtgtg gcgatgacgg
     5641 tatcgcctgc ataaacggta tgcggtcgca gcttgggcga ggcggaaggc tgatcgttca
     5701 actcaatcat tttgccgccg gtcagcaggt tgttgctgga gatggtggcg gtcaggcctt
     5761 tgtttaaggc cgtctggaat tgttgtttcc aatgctcttt gctttgctcg tcggcattga
     5821 tttccaaacg ggaaggctcg atgcggatgc gtacgggaat ccagccgttt tcaaacaggt
     5881 gcaggctgtc attgcggtca aaataaggga catcggaaac catgccgaca ttcagccctt
     5941 tgtattcgac aggcgaaccg acggtcagtc cgcgcacgga ttgtttgaaa aacgcggtgt
     6001 agtacagcga gcggtcgtca ggcaggttgg cgatttcgct gcggctgtcg taaagcgtga
     6061 agctgtcctc gcttttgacg tttttactgt ttttggtttt cggcgaatca aatgaaatcg
     6121 cgcctgacag cagggcaggc agaggggcgg aattgagttt gatgccgctg cctgtggttt
     6181 cgatattgat gccgctttcc agccaaaaac ggctggcgga atgaatcagt ttgtcgttgg
     6241 ggctttggat gaagatggtg taatgcacgc tttggtcgga cgggtcgaaa tgcgcgcttt
     6301 cgatttgccc gaccataaag ttttcataca aaacagggct gttgacgttg aggatgcggt
     6361 cgtttttacc aatcaaattc aagcgcagcc cgctttgccc gatggcggta acgggcggaa
     6421 tgtcctgcac ttggaacacg tcttttgcct cgccgctttt gccgggtgta aaggcgatgt
     6481 acgaacccga aagcagcgta cccaaaccgg ttacgccgct ttggtcgata cgcggcttga
     6541 ccacccagaa ctgggtatcg ctgcggatga ggccggatac gtccgcattg agttgggcag
     6601 taacttccac gcctttttgg tcgtcgcgca gtttgattcg ggtaacgcgt ccgacatcga
     6661 tgctcaatac cttaatgacc gtattgttga cttcgatgcc ttccgcgctg tccatcaaga
     6721 gcgtaaccac aggccccctg ttgcggattt ccttaaccca aagccagccg ccggcaatca
     6781 gcgcgatcag cgggaccagc cagacggcgg agaggaaggt gttgtttttg cggacgcgtg
     6841 cttgagcgtg tccgtttgga ggagggctgt tgtcagtcat atttttccgt ttcgttgaaa
     6901 gcaatgccgt ctgaagcgcg tttgtcccaa agcaggcgcg ggtcgaaata ataggcggac
     6961 agcatcgtca aaatcacgac caggcagaaa tagactgccg cactgcccgg aatgacgcgc
     7021 gcggcataag tgtggaacga acacatcaaa ataataatca caaaaatatc aatcatcgac
     7081 cagcggccga ccgcttcggt gatgcggtag aggtgcgaca atttctttgc gcccgccggc
     7141 aaagcgaacc gtgccgccgc aatcaaaacc gacattgccg caatcttcag caccggcacc
     7201 aaaatactcg cgctgaaaat aaccgccgca atcagcctgt cgccctcgtc ccacatataa
     7261 gcgatgccgc taaagatggt gttggcctcc gtggcggcag gattggacga aatcataatc
     7321 ggcaggatat tggcagggaa atacaaaaca accgccgccg tcagaaacgc cgacgaaata
     7381 ctcagacttt tcggccgtcg gcggtacagt tccgcgccgc acaccccgca gggggattcg
     7441 gcactgtcgc ggaaatacag gcagcggctg caacaggttt tgccttccga tgccgtctga
     7501 accgcattat tccccgtcag ccgcccgatt tggaaataca cccaatgctg gggaaccgat
     7561 accgaagtcc gaatcagcat aaccgacagc gcgaacatca gataaaacgc cggcccgaag
     7621 cgaacctttg ccacagacga gagcttgata tacgccacca gagtggaaac aaaaaacaca
     7681 tccaccatca tcgcctgcct caagcgcacc atcacacgcg ttgccaaacg cagcgcagga
     7741 tacgcctgtt tccgtatcag cgcggcatag acatacaggc acagcagcag aaacagaacc
     7801 ggcgcgccga aagtcagcac aaacatcact tcggccaaaa aaccataatc ctgaaacacc
     7861 atcaggcgca tcatctcggg cagcgaaagg acggatgccg cacccggtat cccgacctcg
     7921 atatacgtca tactgtaagc aaacgccatc aaaatcagcg aagccgccgc ataggcgggc
     7981 ggaccggaaa aagggtggct gcccaccctg aagagtttgt gtccgcaacg gggacagaac
     8041 gccgcctctc ccttgtccaa ctggggcacg tctgtacggc agccgcattc cgggcaatct
     8101 accgtatgcg gcggcagggc ttcgtcgcgg cagttgccgc gaaggctgtg ggggatattt
     8161 tcggcaaacg gcttcatcgg gaaagtggca aatataaatg gcggcattat aaaggaaaaa
     8221 aagcgataca cccaatcagg atgcaaaacc ggcaggttcg gcagtatgaa caaacccttg
     8281 gtgtccggca aaaatatttt ttgagcggct gcccctccgg ccgacaagcc tatttgataa
     8341 aaccccgatt ttgtaaataa tcataaaata cccgcttaaa aatataacgc aaatgatatt
     8401 ttttcgtaaa atatgtgata ataaatttcg tttatgattc tttattattt ttgtatgcgg
     8461 gcaagtccgg attttgcccg caccgcatcc ttatccctta ccgacaaagg agaacttatg
     8521 agcgaaaccg aaaaccaagc cctgacgttt gccaaacgcc tgaaggcgga taccacggcg
     8581 gttcacgaca gcgtggataa cctcgttatg tctgtccaac cgtttgtcag caaagaaaac
     8641 tacatcaaat ttttgaaact gcaatccgtg ttccacaaag cggttgatca tatctataaa
     8701 gaccccgagt tgaacaaagc catttccgag ctggaataca tggcgcgata cgatgccgta
     8761 acgcaagact tgaaagactt gggcgaagag ccttacaaat tcgacaaaga attgccacac
     8821 gaaaccggca acaaagccgt cggctggctt tattgcgccg aaggatccaa tttgggcgcg
     8881 gcatttttgt tcaaacacgc gcaaaagctg gattacaccg gcgaacacgg cgcgcgccac
     8941 ctcgctccgc atccggacgg gcgcggcaaa cactggcgcg ccttcgtcga gcacctgaat
     9001 gccctgaact taactccgga agcagaagca gaagccattc aaggcgcgca ggaagcgttt
     9061 gctttctata aagtgattct gcgtgaaacc ttcggtttgc cggaaggaac agaagccccc
     9121 gaaggcatga tgccgcacag acatta
//
LOCUS       DAXDXW010000084         8697 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXDXW010000084 DAXDXW010000000
VERSION     DAXDXW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.085"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..258)
                     /locus_tag="ACFB64_001827"
     CDS             complement(<1..258)
                     /locus_tag="ACFB64_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210132.1"
                     /translation="MNTKTELQKLLEEDISTLKETLIRVDALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYS"
     gene            complement(320..2944)
                     /gene="alaS"
                     /locus_tag="ACFB64_001828"
     CDS             complement(320..2944)
                     /gene="alaS"
                     /locus_tag="ACFB64_001828"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFC1210133.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGKEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVQEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGGQVVELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFAGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTDAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALRETVTDLTGKSDNA
                     VILLAAVNEGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAD
                     KLPEMLASAEGWLCQKLS"
     gene            3148..4278
                     /locus_tag="ACFB64_001829"
     CDS             3148..4278
                     /locus_tag="ACFB64_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC1210134.1"
                     /translation="MVKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSMIPNYKHLNPEMMRLMDGVDPDHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFNPEYTFKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARDLMEDE
                     FKNDNTIFPSGEDLKNSFIMVPIRPAALKFMVRQWQDVKAGK"
     gene            complement(4319..5221)
                     /locus_tag="ACFB64_001830"
     CDS             complement(4319..5221)
                     /locus_tag="ACFB64_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC1210135.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTVMNVLIKEASAKFALGSGELVF
                     WRMLFSTVTLGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLTTGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQEPAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSATGVAMSSVWATLTGWHTLSFPSAVYLSGI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPIAFKQRLQALFRQR"
     gene            5360..5857
                     /locus_tag="ACFB64_001831"
     CDS             5360..5857
                     /locus_tag="ACFB64_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HFC1210136.1"
                     /translation="MAGMKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAP
                     AKGFRLAPSHWADVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDS
                     MYEIYLRSAVDSQRGEINTEQSKLYIENALRGWQQRWKNMDAKPDNPAFTNFLMEVMK
                     MQPLK"
     gene            complement(5919..6824)
                     /gene="mtrA"
                     /locus_tag="ACFB64_001832"
     CDS             complement(5919..6824)
                     /gene="mtrA"
                     /locus_tag="ACFB64_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HFC1210137.1"
                     /translation="MDILDKLVDLAQLTGSADVQCLLGGQWSVRHETLQCEGLVHIVT
                     AGSGYLCIDGETSPRPVGTGDIVFFPRGLGHVLSHDGKYGESLQPDIRQNGTFMVKQC
                     GNGLDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESEKPLTGTVS
                     VVNALPSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNIDKM
                     VAAANMSRAQLMRRFKSQVGLSPHAFVNHIRLQKGALLLKKTPDSVLEVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            6953..7288
                     /locus_tag="ACFB64_001833"
     CDS             6953..7288
                     /locus_tag="ACFB64_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC1210138.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            7419..7889
                     /locus_tag="ACFB64_001834"
     CDS             7419..7889
                     /locus_tag="ACFB64_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210139.1"
                     /translation="MKYHRLALFAAISCLLLSAVFIAPYLTAFHEQEKIFEYADLTVT
                     APNRSGRAIKLEADGRQYRLSCYGFDSLCTGGNIGRAIRARQVKIVLSETVGKGFLNG
                     VLLEYRNSGSVYSNKDFSRTEDRLVEVLAQPAVFSLKPGILLLLPAIFLRLKKM"
     gene            7995..8558
                     /gene="pgsA"
                     /locus_tag="ACFB64_001835"
     CDS             7995..8558
                     /gene="pgsA"
                     /locus_tag="ACFB64_001835"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC1210140.1"
                     /translation="MPWNIPIFLTWLRVLLIPVLIVLFYLPFSWFSEEAVNVAAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRSSVAVAAVGKFKTAAQMLAIFFLLLNFPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
BASE COUNT         2049 a         2147 c         2329 g         2172 t
ORIGIN      
        1 actataaaaa ttaactttat tctctaaggt tgacaatttt tcaaatacca aacttgtatc
       61 aaggatgggc aattctatag ttaaacctat ttcttttgcg agaatattta attgtttgtc
      121 aattaaccat cgacggacaa taggagttgc aatgctacgt acatatcttg gaggaagagc
      181 atctacacga attagtgttt cttttaaagt tgatatatcc tcttctaaaa gtttttgtaa
      241 ctctgtttta gtattcattc taacaatcca aaatataagc catttgaaca agactcaaat
      301 ggcttattaa gatttaaatt taacttaatt tctgacaaag ccaaccttcc gcgcttgcca
      361 gcatttcggg caatttgtcg gcatccgtac cgccggcttg cgccaagtcg ggacggccgc
      421 cgcctttgcc gccgacttgt tcggctgcaa atttaaccag atctcctgct ttgacttttc
      481 cggtcagggc tttggatacg ccggcgcaca gggagacttt accctcgttt actgccgcta
      541 aaagaatcac ggcgttgtcg gattttccgg tcaggtcggt tacggtttcg cgcagggcgg
      601 ctgcgtcggc ttcgatttgt gcggcaacga gtttggccgc gcccaggtct tttgcgtcgt
      661 ccaagagttt ggcgcctgcg tggacggcga gttcggcttt ggcgcgtgcc aattcttttt
      721 ccaatgcttt ggcgtgtgcc gcgcctgctt ggattttcgc cagtacgtct ttttcggttt
      781 gggctttggt ttcggcaatg atgtctttta ccaaacgctc ttgctcttgc gcccatttga
      841 gtgcgttcag gccggtgatg gcttcgatac ggcgtacgcc tgcggcaata ccgccttcgc
      901 tgatgatttt gaagaggccg atgtcgccgg tgcgtgaaac gtgtgtgccg ccgcacaatt
      961 cggtagagaa accgcccatt tgcagtacgc gtacttcgtc gccgtatttt tcgccgaaga
     1021 gcatcatggc gtcggttttt tgcgcgtctt ccatgctcat aatggctgcg ttgacggcaa
     1081 cgttggctaa aacggcttcg ttgacgcggc gttcgacttc agcgatttct tcggcggtta
     1141 ccgcttgggg atgggaaatg tcgaaacggg tggattcggc ggtaaccaaa gagccttttt
     1201 gttcgacgtg tccgcccaat acgtcgcgca gggctttgtg catcaggtgg gtcgcgctgt
     1261 ggttgcgcat attggcattg cggatttcgt catccacttt ggcggtaacg ctgtcgccga
     1321 ctttcagacg gcctgaagtt tgtacgccga attgtccgaa tacggccgct ttgatttttt
     1381 gggtatcgcg tacttcaaag cggttttcgc ctgcgaagat atagccgacg tcgccgactt
     1441 ggccgccgga ttctgcatag aacggggtaa agtcgataac gacggcaccg ctgtcgcctt
     1501 cgttcaattc gacgacttgc ccgccgtctt tgtagagggc gaggactttg gattcggttt
     1561 ggcgttcgct ataaccttta aactcggtgt cttgaccgtc gtaaggcagt tgggcgttgg
     1621 ctttgaagct ttgggcggcg cgtgcgcgtg cgcgttgggc ttccatttcg cgctcgaagc
     1681 ctgcttcgtc cggttcgata ttgcgttcgc ggcagatgtc ggcagtcagg tcgtatggga
     1741 agccgtaggt atcgtagagt ttgaagataa tttcgccgcc gagtgttttg ccgcctttgg
     1801 ccagcgcgtt ttccaacaaa gccataccgg tttccagagt ttgggcaaaa cggctttctt
     1861 cgtttttcaa agcttcttcg atttgcgcct gtttttcttt caattcagga tacgcgccgc
     1921 ccatctcttg aaccaaatcg gcaacgagtt tgtggaagaa cggtttgctt tgacccagtt
     1981 tgtaaccgtg gcgcacggcg cggcggatga tgcggcgcaa tacgtagccg cgtccttcgt
     2041 tggaaggcaa tacgccgtct gcaatcagga atgagcagga gcggatgtgg tcggcgataa
     2101 ctttcaggct gggttcttcc atactgaacg gcgcgccggt ttcgcgggca acggctttga
     2161 gcaggtcttg gaacaggtcg atttcgtagt tgctgtggac gtgctgcatc acggcggcca
     2221 tacgttccaa gcccataccg gtatcgacgg acggcttggg cagtggattc atattgcctt
     2281 gttcgtcgcg gttgaactgc atgaatacgc agttccaaat ttcaatccag cggtcgccgt
     2341 cttcttcggg gctgccggga atgccgcccc agatttcttt gccgtggtcg tagaaaattt
     2401 cggagcaggg gccgcaaggg ccggtgtcgc ccatttgcca gaagttgtcg gacgcgtatt
     2461 tcgcgccttt gttgtcgccg atgcggacga tgcgttcaga cggcataccg atttcgttca
     2521 accagatgtt gtaggcttcg tcgtcttctg cgtaaacggt cgccaagagt ttgtctttgg
     2581 ggatgttgag ccattcgggg gaagtgagaa actcccaagc gaagtggatc gcgtcgcgtt
     2641 tgaagtaatc gccgaaggag aagttgccca tcatttcaaa gaaggtgtgg tggcgggcgg
     2701 tgtagccgac gttttccaag tcgttgtgct tgccgcctgc gcgtacacat ttttgcgcgg
     2761 tggtggcgcg gctgtacggg cgtttgtcga aacctaaaaa tacgtcttta aactggttca
     2821 tgcctgcgtt ggtaaacagc agggtcgggt cgtcgtgcgg cacgaggctg gaagagcgga
     2881 cgacggtgtg gcctttggtt tcaaaaaatt ttaggaattt ttggcgcagt tcggaggttt
     2941 tcataatttt ttcaatgtct ctcaaatgtc ttgtcatggt aaaagcaggg aaaacgaacg
     3001 gcggtatatt accgcaaatc cctgtttcta gctatggaag cggcggcttt cagacggcat
     3061 tgcgggattt tgaatgccgt ctgaagccct gttaccaata tcggctataa tggccgcttt
     3121 ctccaacccg atatgcaagg aatgataatg gtcaaacatc tgccactcgc cgtcctgact
     3181 gctttgctgc ttgcagcgtg cggcggttcg gacaaaccgc ctgccgaaaa accggcaccg
     3241 gcggaaaacc aaaacgtatt gaaaatttat aactggtcgg aatacgtcga tccggaaacc
     3301 gttgccgatt ttgaaaagaa aaacggcatc aaggttactt atgatgtgta cgacagtgat
     3361 gaaacgctgg aaagcaaggt gctgaccgga aaatccggtt acgacattgt cgcgccgtcc
     3421 aatgcgtttg tgggcaggca gattaaggca ggtgcgtatc agaaaatcga taagtcgatg
     3481 attcccaatt ataaacatct caaccctgaa atgatgaggc tgatggacgg ggtcgatccc
     3541 gaccacgaat acgccgtgcc gttttattgg gggacaaata ccttcgccat caataccgaa
     3601 cgcgtgaaaa aggctttggg tacggacaag ctgccggaca accagtggga tttggtgttc
     3661 aaccccgaat acacgttcaa actcaaacaa tgcggcatca gctatttgga cagcgcggcg
     3721 gaaatttatc ccatggtgtt gaactatttg ggtaaaaacc cgaacagcag caatacggaa
     3781 gacatcaggg aggcaaccgc cctgctcaag aaaaaccgcc ccaatatcaa acgctttact
     3841 tcgtccggct ttatcgatga tttggcgcgc ggcgatacct gcgtaacaat cggtttcggc
     3901 ggagatttga acatcgccaa acgccgtgcc gaagaagcgg gcggcaagga aaaaatccgc
     3961 gtgatgatgc cgaaagaggg cgtggggatt tgggtggatt ctttcgtgat tccgaaagat
     4021 gcgaaaaacg tcgccaacgc gcacaaatac atcaacgact tcctcgatcc ggaagtgtcg
     4081 gcgaaaaacg gcaatttcgt tacctacgcg ccttcgagca agccggcgcg cgatttgatg
     4141 gaggacgaat ttaaaaacga caatacgatt ttcccgagcg gggaagattt gaaaaacagc
     4201 tttatcatgg tgcctatccg gccggcggca ttgaagttta tggtgcgcca gtggcaggat
     4261 gtgaaggcgg ggaaataaag cccgatatgc cgtctgaagg atgttcggac ggcatttttt
     4321 atctttggcg gaagagggct tgcagccgct gtttgaaggc aatggggcgg atgctgctca
     4381 aaatgccgct gaggataatg atgcacatac cgagtatttc ctgccagaaa agctcttcgc
     4441 ccagaaaaaa tgcggcagac agggcggaaa agacgacggt catataggaa agcgaggcaa
     4501 ccgtgaattt gtcgccgact ttgtaggcgc gcgtcatcga cagttgggca atcagcgcgg
     4561 acacgccgat gcccgacaga taaaccgccg atggaaagga cagggtgtgc cagccggtca
     4621 gcgtcgccca aaccgacgac atcgccacgc cggttgcgga aaggtaaaag acgacgcgcc
     4681 agccgggttc gcccgccaaa gacagttcgc gcactttcaa atacgcccag ccggacatcg
     4741 cgccgcccgc cagcccggcg agtgccgccg gttcctgacc gctgcggaac gagggattaa
     4801 gcagcaatac cacgccggca aaaccaagga gcagcaccgc ctgcgtgtaa acggaaatcc
     4861 gttctttcaa aatcaggaag gaaaataccg ccaaaaaaat cgacgaggtg taactcaggg
     4921 taacgccggt tgtcaaaggc agatgcgtta ccgcgtaaaa cagcagcagc atcgcccccg
     4981 tcccgaccat actgcggttt aagtggtttt tccaatgggg cgtgcggaag gtgtcgcgcc
     5041 gcaatacggc ggcagcaccg agcgtaacgg ttgaaaacag catgcgccaa aagaccaatt
     5101 cgccgctgcc gagggcaaat tttgccgatg cctctttaat caatacgttc ataacggtga
     5161 agcaggccgc cgccaccagc atccagcccg atcctaaaat gtcttttttt gcggtatcca
     5221 taaacggtcg ttgcgataag gacggtcgga ttgtaaacct tgcggcaagg cttgtggaat
     5281 gtgtttttgc ctgcttctga tgccgaaatt ttatttttct tgccgaacaa tttgttttct
     5341 caaggcaaac ttgattataa tggcgggtat gaaaaaatac cttatccctc tttccattgc
     5401 ggcagtcctt tccggctgcc agtctattta tgtgcccaca ttgacggaaa tccccgtgaa
     5461 tcctatcaat accgtcaaaa cggaagcacc tgcaaaaggt tttcgcctcg ccccttcgca
     5521 ttgggcggat gttgccaaaa tcagcgatga agcgacgcgc ttgggctatc aggtgggtat
     5581 cggtaaaatg accaaggttc aggcggcgca atatctgaac aacttcagaa aacgcctggt
     5641 cggacgcaat gccgtcgatg acagtatgta tgaaatctac ctgcgttcgg cggtagacag
     5701 ccagcgcggc gaaatcaata cggaacagtc caagctgtat atcgagaatg ccttgcgcgg
     5761 ctggcagcag cgttggaaaa atatggatgc caaacccgat aatcccgcat ttaccaactt
     5821 tttgatggaa gtgatgaaga tgcagccctt gaaatgacgc ggtacgcaaa tgccgtctga
     5881 aagctttttc agacggcatt tgcgtttgaa gccccgattt atttttgccc gccttctttc
     5941 cggtattgcc ccggcgaaac gtgatattgc cgtttgaacg ccttgccgaa atgcgtttcc
     6001 gactgaaagc ccaccgacag cgcgacctcc aaaaccgaat ccggggtttt cttcagcagc
     6061 aatgcgcctt tttgcaggcg gatatggttc acaaaggcgt gcgggctgag tccgacttgg
     6121 cttttgaagc ggcgcatcag ttgcgcgcgc gacatattgg cggcggcaac cattttgtca
     6181 atattccatt cgtcttccgg tttgtctatc accttttgga tcaaatgtcc caaacgtttg
     6241 tcctgccaac ctttcaatac gcccgagagt tcgacatcct tatcctgttc gagataggcg
     6301 cgcaggataa gcaccagcag gacggacggt aatgcgttga ccacggaaac cgtccccgtc
     6361 aaaggttttt cgctttccag ttgcagcatt gaaaccacat actgcaaact tggatgggca
     6421 atgttcagaa aaacggtttc cggcagcccg ttcatcaaat cggcgtgggt gtcgtagcgg
     6481 aaacgggcgc aaaacaggct catatccagc ccgttgccgc actgtttgac cataaatgtg
     6541 ccgttttgcc gtatgtccgg ttgtaaactt tctccgtatt ttccgtcgtg gctcaacaca
     6601 tgacccaagc cgcgcgggaa aaatacaata tcgcccgtgc cgaccggacg cggggaagtt
     6661 tcgccgtcga tgcagagata accgctgccc gccgtaacaa tgtgtaccag cccttcgcat
     6721 tgcaaggttt catgccgtac cgaccattgt ccgcccaaaa ggcactgcac atccgcactg
     6781 cccgtcaatt gggcgagatc gaccagtttg tccagaatgt ccataaatct ttttgaacca
     6841 taaaatgaga tgattaaacg agaaacacaa ctgaataatt gcaataatac gcacatcaaa
     6901 aacagatacg caagcgcgta tcagggttaa acgcaagaga aaggaaaaga aaatgtttaa
     6961 agattggaaa gaacataccg cattggttaa aaaatcattc ggcgagctgg gtaaggcgca
     7021 tcctaaaatg ctgcaagcct acggcgcatt ggaacaggcc gccgccgccg aagcactcga
     7081 tgccaaaacg cgcgaactga ttgccatcgc cgttgccatc accacccgtt gcgagagctg
     7141 catcagcgtt cacgccgccg ccgccgccaa agccggtgcg accgacagcg agatcgcagg
     7201 tgcattggca accgccattg ccctgaatgc gggtgctgct tacacttacg ccctgcgtgc
     7261 attggaagcg gttgaaacgc aaaaataatc cgtttcggat aagaaatgcc gtctgaaaat
     7321 atttcagacg gcattttgcc tgttatgctt cccgatattt tgcagacaac atatgcagcc
     7381 gtttttttca gacggcattt gagaaacatg gaacaagtat gaaatatcac cgcttggcat
     7441 tgtttgccgc cataagctgc ctgttgttgt ccgccgtttt tattgcacct tacctgacgg
     7501 catttcacga acaagaaaaa atatttgaat atgcggattt gaccgttacc gcccccaacc
     7561 gcagcggacg ggcaatcaaa ttggaggcgg acgggcggca gtaccggctt tcctgctacg
     7621 ggttcgacag tttgtgcaca ggcggcaata tcggcagagc catcagggcg cggcaggtta
     7681 agattgtttt aagcgaaacc gtcggcaaag gttttttaaa cggcgttctg ttggaatacc
     7741 gcaacagtgg cagtgtctat agcaataaag atttttcccg cacggaagac cgccttgtcg
     7801 aagtgttggc acaacctgcc gttttcagcc tgaaaccggg tattttgctt ttattgcccg
     7861 ccattttcct gcgtttgaaa aaaatgtgaa aacagattgg cggggcgggg gaatgactat
     7921 gcggcaattt tacgttttgg gttatgagga taaccgttat aatcataatt ttacagtccg
     7981 cacagagaaa accgatgcct tggaacatcc ccatcttcct cacatggttg agggtcttgc
     8041 tcattcccgt actgattgtc cttttctacc tgccgttctc atggttttca gaagaagcag
     8101 tcaatgttgc cgccgccgtc atttttgccg ttgccgcctt gaccgactgg tttgacggat
     8161 ttttggcaag gttgtggaaa cagacttccg atttcggcgc attcctcgac cccgtcgccg
     8221 acaagctgat ggttgccgta tcgctgctgc tgctggtcaa actcgaccgg acctatgttt
     8281 tgttcgcgat gattatcatc ggcagggaaa ttaccatttc cgcattgcgc gaatggatgg
     8341 cgcaaatggg aaaaaggagc agcgttgccg tcgccgccgt cggcaagttc aaaaccgccg
     8401 cgcaaatgct ggcgattttc tttttgctgc tgaattttcc cgatttttac ggatttaatc
     8461 ttgttgttat cggcaacata ttgatgttta tcgcatcttt gctgacagtc tggtcgatgc
     8521 tgtattatct gaaaatggcg tggaaagaaa ttgcctgaaa aaaacataaa aatagcttga
     8581 cggtaaaaac ataatccata ataattgcgt cttcttcgat gtcggagagt gaaatcgggc
     8641 gggaatagct cagttggtag agcgcaacct tgccaaggtt gaggtcgcga gttcgag
//
LOCUS       DAXDXW010000085         8059 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXDXW010000085 DAXDXW010000000
VERSION     DAXDXW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.086"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            1..223
                     /locus_tag="ACFB64_001836"
                     /pseudo
     CDS             1..223
                     /locus_tag="ACFB64_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749744.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
     gene            333..1010
                     /locus_tag="ACFB64_001837"
     CDS             333..1010
                     /locus_tag="ACFB64_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFC1210141.1"
                     /translation="MTFFKPFTVVLTASALALSGCVADPVTGQQSPNKSAMYGLGGAA
                     VCGIVGALTHSGKGARNSALACGAIGAGVGGYMDYQEQRLRQNLAGTQIEIQRQGNQI
                     RLVMPESVTFATGSAALGGSAQYALNTAAQTLVQYPDTTLTINGHTDNTGSDAVNNPL
                     SQHRAQAVAYYLQTRGVAASRLTVYGYGSHMPVASNATVEGRAQNRRVEILINPDQRA
                     VNAARHM"
     gene            complement(1284..2054)
                     /gene="xth"
                     /locus_tag="ACFB64_001838"
     CDS             complement(1284..2054)
                     /gene="xth"
                     /locus_tag="ACFB64_001838"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC1210142.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIISRSVPQDVHFGLPSLPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWYEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVTSEMAAVLKDVRIDLETRALERPSDHAPVAAEFDL"
     gene            complement(2114..2395)
                     /locus_tag="ACFB64_001839"
     CDS             complement(2114..2395)
                     /locus_tag="ACFB64_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFC1210143.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIVELAKSLSLTAT
                     AVSCHLNRLRAGGLVDFTRYHRIIEYRLVSEDAATILRTIRDLENKRAA"
     gene            2648..2899
                     /locus_tag="ACFB64_001840"
     CDS             2648..2899
                     /locus_tag="ACFB64_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210144.1"
                     /translation="MDIFRESRPFCATGCPTPTSGSAFHHRRRRYGQDRIGRAFLTMH
                     RILDKENQIHTDMALALPAVVKDAVAIWADMDALDRCSG"
     gene            complement(3130..4011)
                     /gene="nadC"
                     /locus_tag="ACFB64_001841"
     CDS             complement(3130..4011)
                     /gene="nadC"
                     /locus_tag="ACFB64_001841"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC1210145.1"
                     /translation="MPSENPLFALPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPCVRFQAEIQDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     ANHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            complement(4087..5025)
                     /locus_tag="ACFB64_001842"
     CDS             complement(4087..5025)
                     /locus_tag="ACFB64_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210146.1"
                     /translation="MDAYPEAEAPLQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQKRLKHIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQTMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            5227..6339
                     /gene="nadA"
                     /locus_tag="ACFB64_001843"
     CDS             5227..6339
                     /gene="nadA"
                     /locus_tag="ACFB64_001843"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC1210147.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDHETAGRL
                     KDEIKALLKRKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVAVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKHALTGGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            6391..7899
                     /gene="nadB"
                     /locus_tag="ACFB64_001844"
     CDS             6391..7899
                     /gene="nadB"
                     /locus_tag="ACFB64_001844"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC1210148.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIGKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NHNDLHLTREGGHTCRRIAHVADYTGEAVMQSLIIQIRRRPNIRVYERQMALDVQTES
                     GAACGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     IENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQSGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISRQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQG
                     IADGQAFQIEPFQRPSESPSAETDIFSNDLQNTFSRPVLQTFNQRHLGILRNDTDLHR
                     AIDQLRLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT         1749 a         2371 c         2269 g         1670 t
ORIGIN      
        1 tcctaaaccc accatccgcg cctgccgcac gtacacgtcg acaaaccgaa aatatggctg
       61 ctcggcggca tcgtcggcgc gtggggctgc cataaaatcg gacaccattt ctccctgccc
      121 gtttcggcat tattgccgat accgggtgca ggctcggtcg gctacgatgt gaaggtacgt
      181 ttttttgcgg atgtggctgc ggtttcggcg caaatcacga taaacccgcg ttgtttcggg
      241 aaaaatcggg taatttcagg ttaattgcac tttttcagac ggcatgattg cttatagttg
      301 tacggtttga catatgcacg gaaggaaacg ccatgacttt cttcaaaccc tttaccgtcg
      361 tgctgaccgc atccgcactc gcgctttccg gctgcgttgc cgaccccgta accggacagc
      421 agtccccaaa caaatccgcc atgtacggtt tgggtggcgc ggcagtgtgc ggcatcgtcg
      481 gcgcactgac ccacagcggc aaaggcgcac gcaattccgc gcttgcctgc ggcgcaatcg
      541 gcgcaggcgt gggcggctat atggactacc aagagcagcg tttgcgccaa aaccttgccg
      601 gcacgcaaat cgaaatccaa cgccaaggca accaaatcag gctggtgatg cccgaaagcg
      661 ttaccttcgc caccggcagc gcggcgttgg gcggcagtgc gcaatacgcc ctgaacactg
      721 ccgcacagac gctggtgcag tatcccgaca cgacgctgac catcaacggg cacaccgaca
      781 acacaggttc cgatgcagtc aacaatccgc tttcgcaaca ccgcgcccaa gcggttgcct
      841 actatctgca gacgcgcggc gtggcggctt cgcgcctgac ggtttacggc tacggttcgc
      901 atatgccggt cgcgtccaac gctacggttg aaggccgcgc gcaaaaccgc cgcgtcgaaa
      961 tcctcatcaa ccccgaccaa cgcgccgtca acgccgcacg gcacatgtaa cgcgccctgc
     1021 aacgccaatg ccgtctgaat gtttttcaga cggcatttcc gtattcgcaa gcacagccgt
     1081 accccgactg taatgctatg gataacgtct cgccgtttgc cgggaaaccg cagattgcag
     1141 ccggtggcgt gcaagtgggc ccaaccttgc agacgttacc gccataccgc gatacccctg
     1201 taacctcatc gtttaaacag atattcaatc aaaatgccgt ctgaaaccct gcgaagtttc
     1261 agacggcatt tgcctttccc gaattacaaa tcaaattctg ccgccacggg cgcgtggtcg
     1321 ctcggacgct ccagcgcgcg cgtctccaaa tcgatgcgga catccttcaa aacggccgcc
     1381 atttcggatg taaccaatat atggtcgata cgcaggccca gtttgcgttg gaacatcgcg
     1441 ccgcgatagt cgaaccaggt atagaacgcg ccttcgggat ggacttggcg caggctgtcg
     1501 gtcaatccca aatccagcag gttttgaaac cactgccgtt cgacggacga acagtggatt
     1561 ttttcatacc atttttcagg gtcgtaacag tccgcatcgg caggcgcgat attgaaatcg
     1621 cccagcagca ccagtttgcc gtggcgggtc atttcgtcgc ggacaaactc cgtcagtgcg
     1681 gcaaaccact gttccttata tttgaatttg gggctgtcca aagcctcgcc gttgacgcaa
     1741 tagacattga tgacgcgcac gccgcctacg gtcgccgcaa tcacgcggcg ttgcgggtca
     1801 tccggcaggg aaggcaaacc gaaatgcacg tcctgcggca cgctgcggct gatgatcgcc
     1861 acgccgttgt aggttttctg cccgctccaa acacagtgcc agcccatcat ctgcaaggcg
     1921 gcggccggaa atttgtcctg atcgagtttg agttcctgca aaaccaaaat atcgggcgga
     1981 ttgtcggcaa gcaggttttg cacctgcggc agccgcacat tgagcgaatt gacgttccaa
     2041 gtggtgattt tcataacatt tccgaaaaat gccgtctgaa acgttcagac ggcaaaagaa
     2101 aaggattcta acactatgcc gcgcgtttgt tttccaaatc gcggattgtc cgcagaatcg
     2161 ttgccgcgtc ttcggaaacc aggcggtatt cgataatgcg gtggtaacgc gtaaagtcca
     2221 ccagaccgcc cgccctcagg cggttcaaat gacaggaaac cgcggtggcc gtcagggata
     2281 aggattttgc cagttcgacg atattgcgtt cgctgtccaa cagttggatc agtatcgcca
     2341 tacgttcggg atgcgccata agtttgagta tggtgtggag cggtattgtg ttcatcggta
     2401 ttcctaaaac cttttccgcc cgccaagcgg attgacgggc ggcagatatg gatgattttg
     2461 attatcttta taaattttat tataaatcaa ttgtttattt ctcattgtac aggattgtat
     2521 gttccttttc ccactatttt tgcaaacaag cgtaaagaca ggctttcaga cggcataaaa
     2581 ctgccgcccc taccctgcct caccctgttg ccgaacggaa tcatccgtat tttttaagaa
     2641 aaccattatg gatattttcc gtgaatcaag gccgttctgc gcgacaggct gtccaacgcc
     2701 aacatccggt tcggcatttc atcatcgccg ccgacgatac gggcaagacc gcatcggtcg
     2761 tgccttccta acgatgcacc gcatcctcga caaggaaaac cagatacata ccgatatggc
     2821 tttagcgttg ccggcggtag tgaaagacgc agtggcgata tgggcagata tggatgcatt
     2881 ggataggtgt tccggctgac cgctccgtta ctgccgtatc tttcggctac cgttcatcaa
     2941 atggttccaa tgccgaaatg cctaaatcgc gcctgcctga aatgctgttt tttctaaagc
     3001 atcacgacct tcacgaaaaa tgccgtctga agaatatttc agacggcatt ccctccctga
     3061 ggcaataacc ggattacggt taagcggttc cttacctgac atatcagaca gccgcccgca
     3121 ccctgaaact cacgccacga aatccaaacc tatgtccaac gatcggctgc tgtgggtcag
     3181 atagccgagg gcgatgccgt ccacgccggt ttgcgccacg cgcttcaggc ggtcgaagcc
     3241 gatgccgccc gatgcttcgc aatagacggt gtgtgggtgg gcggtttgtg tgtggcagcg
     3301 gtttgccgct tctttcaggg tttcgtcgtc catgttgtcc agcaaaatcc gttccgcgcc
     3361 cgctgcgatg gcttcgtcca gttgtgccaa tgtgtccact tcgatttcca cgcaggtcaa
     3421 cggtccgacc gcctgttttg cctgccgaac ggcttgggcg atgctgccgc aataggcgag
     3481 gtggttgtct ttgatgagca cggcgtcgtc caaacccatg cggtggttcg cgccgccgcc
     3541 cgccctgacg gcgtattttt gcaggacgcg cagcaggggg atggttttgc ggctgcacac
     3601 gatgtctgta ccgtattcgg cgacctcggc aacggcacga gcggtggcgg tggcgatgcc
     3661 gcttaagtgc gtgaggtagt tgagcgcggt gcgttcggcg gcgagcagcg cgcgggcgtt
     3721 gccttcgacg gcagccagtg tctgacctgc gcggacggct tgcccgtctt ggatttcggc
     3781 ttggaagcgg acgcacggat ccatcgtctg aaaggcgagg cgcgccaagt ccataccggc
     3841 gataacaccg tcttcgcggc tgacgaggaa aagtttggcg gttttgtcgg gcgcgatgac
     3901 ggcggcggac gtaatgtcgc cgcgcctgcc caagtcttcg ctcaaggctt gttccaccat
     3961 agggcgcaac aaggtgtcgg gcagggcaaa gagggggttt tcagacggca tggggtttcc
     4021 tttgttaaaa aagattgatg ttgcaccgat ggggcaaaac ttcatttgat gccgtctgaa
     4081 aacgggctaa cggctgccca gcggcagtcc gatgtccgaa atcagcctgt cgggcaggac
     4141 gtcgtcgcgg aagcggcaaa gctgcgcggg acggcctttg ctgcccgata cgcctgtatc
     4201 cgacggctcg atgaggtttt gctgctgaat ctggcggcgg aagttttgct tgtgcagcaa
     4261 tctgccgctg atggcttcga cgctgttttg cagttgcagc agcgtgaatt cgggcggcat
     4321 cagttcgaaa atcacgggac ggtatttgat tttggcgcgc aggcgggaca gggcggtcgc
     4381 caacacgcgg cggtggtcgt ggcgcatggt ctgccccgtg agcgcgaagt cgaagtttgc
     4441 ctgcggctcg gcggcctccg ctatcagacc gctttcatac aacatttcat agcgttgcaa
     4501 aacgtattct tccgaccagt tttccggttc gaccccccaa cacaaatgaa tatgcttgag
     4561 ccgcttttgg cggacttcct ccgtgtccgc cgagtttgcc caaatgcgca ggcggctgac
     4621 gacggcgtcg cgctgcccgc cgtcggtgcg caagtcttcc catgggaaat agccgtagca
     4681 gtcctgccat ttcgcgtcgg gatgcaggat gctatcggct gcctcgcgca ccagccccaa
     4741 atagctgacg tacagcacgg gcatgccgtg ttcgttgcgg cggtgggtat cgacaaaggt
     4801 gtaaagctgt tccacatagc ccataggctg cgaagtctgc ttggcgaccc acagctttac
     4861 gcccgcctgc agggaattgc gcagggggga aagcgggccg ttgggcagga gcgtgccttg
     4921 ggcgacggtc aacacccgca ggccgccgtc ggtaacggca atcaatacgg gaaccagctc
     4981 gacgatgctt tgcagcggag cttcggcttc ggggtaggcg tccatgctcg gctttctgcg
     5041 ggtgggaaag gctttattgt aaaccaaagc ggtttgtcgg aacgaagcgg gtcggttttg
     5101 cggtatcgca aacctgtttt cagacgacct tgcatattat actcaagttg agcataatgt
     5161 aaaaacagtt tgaaaaacag acggcaaacc ttcatgccgt ctgaaaacca gctacaagga
     5221 aacatcatgc aaaccgccgc ccgccgctcg ttcgattacg atatgcccct catccaaacg
     5281 ccgacttccg cctgccaaat ccgtcaggcg tgggcgaagg ttgccgacac gcccgaccac
     5341 gagacggcag gtcgtctgaa agacgaaatc aaggccttgc tgaagcggaa aaacgcggtc
     5401 ttggtggcgc attattacgt cgatccgctg attcaggatt tggctttgga gacgggcgga
     5461 tgcgtgggcg attcgctgga aatggcgcgc ttcggcgcgg aacacgaagc cggtacgctg
     5521 gtagtggcgg gcgtgcgttt catgggcgag agcgcgaaaa tcctctgccc tgaaaaaacg
     5581 gtgctgatgc ctgatttgga agcggaatgt tctttggatt tgggttgtcc cgaagaagcg
     5641 ttttcagcgt tttgcgacca acaccccgac cgcacggtgg cggtgtacgc caatacttcc
     5701 gcagctgtga aagcgcgtgc cgactgggtg gtaacgtctt cggtggcgtt ggaaatcgtg
     5761 tcgtatctga aatcgcgcgg cgagaaactg atttgggggc ccgaccgcca tctcggcgac
     5821 tacatccgcc gcgaaacggg cgcggatatg ctgttgtggc agggttcgtg catcgtccat
     5881 aacgaattca aagggcagga actggcggcg ttgaaggcag agcatcccga tgctgtggtg
     5941 ctggttcatc ccgaatcgcc gcaaagcgtc atcgaactgg gcgacgtggt cggctcgacc
     6001 agcaaactac tcaaagccgc cgtatcgcgc cctgaaaaaa aattcatcgt ggcaaccgat
     6061 ttgggcatcc tgcacgaaat gcaaaagcag gcgcccgaca aagaatttat cgccgcgccg
     6121 acggcgggca acggcggaag ctgtaaaagc tgcgcgttct gcccgtggat ggcgatgaat
     6181 tcgctgggcg gcatcaaaca cgccctgaca ggcggacgca acgaaatcct gttggacaga
     6241 aagctgggcg aagccgccaa actgcctttg cagcgtatgc tcgacttcgc ggcaggactc
     6301 aaacgcgggg atgtgttcaa cggcatgggg cccgcctgat ttgccgtcca tatcgccgtt
     6361 tcagacgacc tctcaaacaa ggacaacacc atgcaaaccg attgcgacgt attgattgcc
     6421 ggaaacgggc tggcggcact gacgctcgcc ctgtcgctgc ctgaatcgtt ccgcatcgtc
     6481 attttgtgca aaaaccggct ggacgacacc gccagccgtc atgcgcaagg cgggattgcg
     6541 gcggcgtggt cgggagagga cgacatcgga aaacacgtcg ccgatacttt ggaagcgggc
     6601 gcaggtttgt gcgacgaagc cgccgtccgc accatcctgt cgcagggcaa accggcaatc
     6661 gaatggctgc tggcgcaggg cgtggcgttc gaccggaatc ataacgacct gcacctgacg
     6721 cgcgaaggcg ggcatacctg ccgccgaatc gcccacgtcg ccgactacac gggcgaagcc
     6781 gtcatgcaga gcctgattat ccaaatacgc cgccgaccga acatccgcgt ttacgagcgg
     6841 cagatggcgt tggacgttca aaccgaatca ggcgcagcgt gcggactgac cgtcctcgac
     6901 tgccgaacgc aagaaaccta ccgcatccgt gcccgccata ccgtactcgc aggcggcggc
     6961 ctcgggcaaa tttacgccgc caccaccacg ccgcccgaat gcacgggcga cgccatcgcc
     7021 atggcgatac gcgcgggttg cgcaatcgaa aacctcgaat ttatccaatt ccaccccaca
     7081 ggcttggcaa ggccgtctga aaacggccgc accttcctga tttccgaagc cgtgcgcggc
     7141 gaaggcggca tcctgaccaa tcaatcgggc gaacggttta tgccgcatta cgaccgccgc
     7201 gccgaactcg cgccgcgcga catcgttgcc cgcgccatcg ccgccgaaac cgccaaacaa
     7261 acgcaagact tcgtctcgct cgacatcagc cgccaacccg cagcgttcgt ccgtcggcat
     7321 ttcccgtcca tccatcggca ctgcctgtcc caatgcggct tggacatcac gcgccaagcc
     7381 atccccgtcc gccccgtgca acactacacc tgcggcggca tccaaaccga ccccagcggc
     7441 agaacctccc tgccgcagct ctacgcctta ggcgaaaccg cctgtaccgg actgcacgga
     7501 gccaaccgcc tcgccagcaa ctccctgctc gaatgcgtcg ttaccgccag gcttgccgcc
     7561 caaggcatcg cggacggaca agcgttccaa atcgaaccat tccaaaggcc gtctgaaagc
     7621 ccctccgccg aaacagacat cttttcaaac gacctccaaa acacattcag ccgccctgtc
     7681 ctgcaaacgt tcaaccaacg ccatctcggc atcctccgca acgataccga cctgcaccgc
     7741 gccatcgacc aactgcggct ttggaagcaa aaccaagccg aaccgcacac cgcgtccgaa
     7801 tacgaaaacc gcaacctact cgaatgcagc ctcgccgtcg cccaagccgc atacaggcgg
     7861 agacagaaca tcggtgcgca ttttaatagt gattgttaag gttggctggt tagaaaaaaa
     7921 tcgttcaaat aaatttgaat agcatggtta agagattggc attcagtttt tatggttgcg
     7981 taagttgggt tgaggccttt gcaaaaaagc ccttccttcg gcatccgaaa cccaaacaca
     8041 ggttttcggc tgttttcgc
//
LOCUS       DAXDXW010000086         7615 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXDXW010000086 DAXDXW010000000
VERSION     DAXDXW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.087"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..1716)
                     /locus_tag="ACFB64_001845"
     CDS             complement(<1..1716)
                     /locus_tag="ACFB64_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFC1210149.1"
                     /translation="MDYLQNLSLGLTKKLPVILQTEVAECGLACLAAVAGFYGFYTDL
                     RALRSKYCLSLKGENLADIVRFADDMGLTGRALRLDLDELGSLRLPCILHWDLNHFVV
                     LESVSSDGAAVMDPASGRRKVKTEEISRKFTGIALELWPNTRFEAGEEKQEIRILPML
                     RGISGLGRTLFQLLALAAAMEVFALVSPFFMQWVIDHVVVTGDRNLLATLALGFTLLL
                     LVQNTVSVMQGWLGMHFSTTLNVQWKSNVFKRLIDLPTDYFAKRHLGDVVSRFGAVDS
                     IQGTLTSTFFVLVLNSLTAVFTFVLMIVYSPILTGLVAATLVVYIAVRWVAYYPLRRA
                     TEENIIHAAKQSSYFMETVRGIRAVKQFGKGPQRYSAWMGLLVDTVNTGLTVQKLGIW
                     FGLANKLLFGLANILIIYLAAGMVLDGVFTVGVLMAFLAYKGQFEGRIGSLVDQFVQI
                     KMLSLHAERLADTVLNETESEATPDVGIPDISDDIEISVENVSFRYADNEPYVLQNVS
                     FKIGRGESLALIGRSGCGKSTFLDILSGNLPPESGKVMINGHDIYSLPPRFIRNLSAM
                     VRQDDV"
     gene            complement(1818..2033)
                     /locus_tag="ACFB64_001846"
     CDS             complement(1818..2033)
                     /locus_tag="ACFB64_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210150.1"
                     /translation="MDFAHYLKHWKAAVLIYLAISILTDILCYFLNFDGVFYKGRFFS
                     VTVAGPVGALSFLAYLLYLKREENRSH"
     gene            complement(2047..2394)
                     /locus_tag="ACFB64_001847"
     CDS             complement(2047..2394)
                     /locus_tag="ACFB64_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210151.1"
                     /translation="MRELTMNEMTAVFGGTEGTWGHRGRVSGEWVGSKVGGALGSFAG
                     AFTGMAGGALVTGGNPAGIAIGGAGGGWVGAEGGSALGGHIGGFAGAKWGKMIDRRNC
                     QCGEDCHCDPCNC"
     gene            complement(2579..3853)
                     /locus_tag="ACFB64_001848"
     CDS             complement(2579..3853)
                     /locus_tag="ACFB64_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC1210152.1"
                     /translation="MNRPKQPFFRPEVAIARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTMEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ISQQIDGQKRRIRLAEEMLRKYRFLSANDAVSKQEMMNVEAELLEQKAKLDAYRREEA
                     GLLQEIRTQNLTLASLPKRHETEQSQLERTIADISQEVLDFEMRSEQIIRAGRSGYIA
                     IPNVEVGRQVDPSKLFLSIVPERTELYAHLYIPSSAAGFIKPKDKVVLRYQAYPYQKF
                     GLASGSVVSVAKTALGRQELSGLGMVSSDLAKSNEPVYLVKIKPDKPTITAYGEEKPL
                     QIGMTLEADILHEKRRLYEWVLEPIYSMSGRL"
     gene            complement(3914..4186)
                     /locus_tag="ACFB64_001849"
     CDS             complement(3914..4186)
                     /locus_tag="ACFB64_001849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210153.1"
                     /translation="MLPQPKEVPHGGILLQYLLLQRQFISCRKIFSVLYGANGVYNLT
                     RDWVNDAVNAPPYNGRPIFEIEHGLTAPATKADKSGNGYTDGTDYC"
     gene            complement(4261..4488)
                     /locus_tag="ACFB64_001850"
     CDS             complement(4261..4488)
                     /locus_tag="ACFB64_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210154.1"
                     /translation="MKNWKQFIFFVILVIACYQLLYSLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQEKEYKND"
     gene            complement(4497..5651)
                     /locus_tag="ACFB64_001851"
     CDS             complement(4497..5651)
                     /locus_tag="ACFB64_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC1210155.1"
                     /translation="MGIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLEAASCKYINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDARDMYAVQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQAKHEEVRKLIYQLIELDFLEIL"
     gene            5903..6184
                     /locus_tag="ACFB64_001852"
     CDS             5903..6184
                     /locus_tag="ACFB64_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210156.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPAGFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            6181..7224
                     /gene="argC"
                     /locus_tag="ACFB64_001853"
     CDS             6181..7224
                     /gene="argC"
                     /locus_tag="ACFB64_001853"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC1210157.1"
                     /translation="MSKKIKAGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGTAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSDPETVLRDYYRDSPFMDI
                     LPAGSTPETRSVRGANLCRISIRQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     EETHGLGGIPLLP"
BASE COUNT         1950 a         2012 c         1689 g         1964 t
ORIGIN      
        1 aacatcgtcc tgcctgacca tcgcactcaa attgcggata aaacgtggcg gtaagctgta
       61 aatgtcgtgc ccatttatca tgactttgcc tgattcggga ggtagattgc cgcttaaaat
      121 atccaaaaat gtcgatttac cgcagcccga tcgtccgatt aacgcaagcg attcaccacg
      181 tccgatcttg aagctgacgt tttgtaaaac atatggctcg ttatcggcat aacggaaaga
      241 aacattttcc actgagattt cgatgtcgtc tgaaatatca ggaatgccta catccggcgt
      301 agcttccgat tctgtttcat tcaatacggt gtcagctaac cgttccgcat gaagtgacag
      361 cattttaatt tgcacaaact gatcgaccag cgagccaata cgtccttcaa actgtccctt
      421 ataagccaaa aaagccatta gtacgccgac ggtaaagacg ccgtccaata ccatgcccgc
      481 agctaagtaa ataatcaaaa tatttgccaa accgaaaagc agcttgtttg ccaagccgaa
      541 ccaaattccc agtttctgca ccgtcagtcc cgtattaacc gtatccacca gcagccccat
      601 ccatgcagaa tatcgttgag gacctttacc aaattgcttg actgctcgga taccgcgtac
      661 cgtttccata aaatagctgc tttgtttggc agcatggatg atgttttcct ccgtcgcccg
      721 tcgcaacgga taataggcta cccaacggac tgcgatataa actaccagtg tcgctgcaac
      781 caaacctgtc agtatcgggc tgtacactat cattagcaca aaagtaaaaa cagccgttag
      841 gctgtttaaa accaacacga aaaacgttga agtcagagtt ccctgaatac tatccaccgc
      901 cccgaagcgc gataccacat cgcccaaatg ccgtttagcg aaataatctg taggcaaatc
      961 tatcaaacgt ttgaaaacgt ttgatttcca ctgaacattt aacgtcgttg aaaaatgcat
     1021 gcccaaccag ccctgcatga cagacaccgt attttgtacc agaagtagca aggtaaagcc
     1081 caaagccagc gtcgctaaca gattacgatc gcccgttacc actacatggt cgatgaccca
     1141 ttgcataaaa aacggcgaca ccaaagcaaa cacttccatt gctgctgcca aagccaaaag
     1201 ctgaaacaat gtccgcccca gcccagaaat cccgcgcaac atgggtagga tgcggatttc
     1261 ctgcttttct tcccctgcct cgaaacgcgt gtttggccac agttccaaag caattcccgt
     1321 aaacttgcgc gatatttcct ccgtcttgac tttgcgtcgt cccgaagccg gatccatgac
     1381 ggcagccccg tccgaagata ccgattccag caccacaaaa tgattcaaat cccaatgtag
     1441 aatacagggc aggcgcaaac tgcccaattc gtctaaatcc agcctcaacg cccgtcccgt
     1501 cagccccata tcatcagcaa aacgaacaat atctgccaaa ttctcaccct taagtgacag
     1561 acagtatttt gaacgcagtg cgcgcaaatc cgtatagaaa ccataaaatc cggccacagc
     1621 cgctagacat gccaagccac actccgctac ttctgtttgc agtataacgg gcagcttttt
     1681 tgtcaagccc aaagacaggt tttgcagata atccatagat ttccttgttg aatcagaaaa
     1741 gagagatggg aagacgggaa tggtttggaa aggccgtctg aaaaggtttc agacggcctg
     1801 cagccccata ttgactttca atgcgaccgg ttttcctcgc gtttcaaata cagcagatac
     1861 gcaagaaaag acaaagcccc tacaggtcct gcgacggtaa ccgaaaaaaa cctgcctttg
     1921 taaaacacac cgtcgaagtt taaaaaatag caaaggatgt cggtcaggat gctgatggca
     1981 agataaatca gcacagcggc tttccagtgt ttcaggtaat gtgcgaaatc catttcaaac
     2041 tcccgatcaa cagttgcacg ggtcgcaatg gcagtcttct ccgcattggc agttgcgcct
     2101 gtcgatcatt tttccccatt ttgcaccggc aaaaccgccg atatgcccac ccaatgcaga
     2161 accgccctca gctcctaccc atccgccgcc tgcgccgccg atggcaattc cggcaggatt
     2221 tccgcctgtt accaatgcgc cgcccgccat tcccgtgaat gctccggcaa acgaccctaa
     2281 agcgccgccg accttgcttc cgacccattc tccggagact ctgcccctgt gcccccaagt
     2341 accttctgtg ccgccgaaaa cagcggtcat ttcgttcatg gtcaattctc tcatttcaaa
     2401 actcctttta tcgtcaatcg aaaaattgaa agggcagata ccctatcctt gcttttacag
     2461 acagagtatc tgtaaaaggg ggtagtttca ggcagcttca gtgtttatgt ttttatgtag
     2521 tgttttacag gtcgtctgaa gaggtttcag acgacttttt atctttcagc ctcaagcctt
     2581 acaacctgcc cgacatacta taaatcggct ccaataccca ttcgtacagc cgccgtttct
     2641 cgtgtaggat gtctgcttcc aacgtcatgc cgatttgcag cggtttttcc tcaccgtatg
     2701 cagtgatggt tggtttgtcg ggttttattt tcacgagata aacaggttcg ttgctcttcg
     2761 ccaaatcgga ggataccatg cccaatcccg acaattcctg tctgcccagt gccgtttttg
     2821 ccactgatac gacactgccg gaagcaagcc cgaatttttg atagggatat gcctgataac
     2881 gcaggacaac cttgtctttc ggcttgataa agcctgctgc actgctgggg atatatagat
     2941 gggcatataa ctcggtacgt tcgggaacaa tgctcaagaa cagtttggaa ggatcaacct
     3001 gccgtccgac ttcgacgttc ggtattgcta tataacccga ccgtcctgca cggatgattt
     3061 gttcagagcg catttcaaaa tccaaaactt cttgagaaat atcggcaatg gtgcgttcaa
     3121 gctggctttg ttctgtctca tgccgtttgg ggaggctggc caatgtcaga ttctgcgtgc
     3181 ggatttcctg aagcagcccg gcttcttctc ggcggtaggc atcaagtttg gctttctgct
     3241 ctaaaagctc tgcctcgaca ttcatcattt cttgttttga cactgcatca ttggcggata
     3301 ggaaacgata tttccgcaac atttcttccg caagtctaat gcgccttttc tggccgtcta
     3361 tctgttgcga aatatggagt ttctggtttt ccaaacgttc gacagttgct ttaaggctgc
     3421 gcgtttcatt ctcgtgtatc agcttcagac gacccagttc ctgttctgcc aacgttttct
     3481 tcaaaactgc ctccgttttc aactgctgct gcacgctacc tcctgcgccg aaacgtgagg
     3541 tcgaaagcgc aaatagcttg tcgccagcct taaccttttc tccatcttcc acgaatttcg
     3601 ctgtaattgt ccccgtatcc ggtgcataca ccctgattac gcccgatgca ggtagaattt
     3661 gtccctccat tgttgtcttt cgcgtatagt taccgaatat caaaaacagg ataatcagta
     3721 acgcagatat cgatgcaaat gtcgtccaca gggaaaatga caacgggcgt gtcagaatca
     3781 ctttacccgt caggctggtt tggcgggcaa tggcgacttc agggcggaag aaaggttgct
     3841 tgggtctatt cataaaattg aagttaagaa agtttcagac gacccctaga gatcgtctga
     3901 acaatgagaa atatcagcag taatctgtac cgtcagtgta gccgtttcct gatttatctg
     3961 cttttgttgc gggagcagtt aatccatgtt caatctcaaa gattggtctt ccgttataag
     4021 gaggtgcatt aacggcatca tttacccaat cacgagtcag attgtataca ccatttgcac
     4081 catatagcac cgaaaaaatt tttcggcagg aaatgaactg tcgctgcagc agcaggtatt
     4141 gcaacaaaat ccccccatgt gggacctcct ttggttgtgg cagcattagc tacatttcca
     4201 gctatattgt ctgttacagg acctccccct gaaaccagct tcaattcatg aagttgaagt
     4261 tcaatcattt ttatactcct tttcttggtt ggtattccta aaaattcggc taacaaaaac
     4321 atatggcaga tatattgaaa aaaaattcaa agtaccctga ataaaattca aattccaact
     4381 atatttgtta atgtagtcga gaagaaacat atctgataaa gaatatagca attgataaca
     4441 agctattact aatattacga aaaatataaa ctgcttccag tttttcatag aatccctcac
     4501 aaaatttcca gaaaatctaa ctctatcaac tgatagatca acttcctaac ttcttcatgt
     4561 tttgcctgat tgaagttaac cagtagattt ttcaacaata acggttcatt cttaccgatg
     4621 tgttctaaca cttttttccc caactcatct acgcttatct tcatcccatt cccaatcaaa
     4681 tatccctttt ccaacgtatc caaattattg gcatttagtc tcaacctgac gtcgtctgaa
     4741 agcggagtag cgttgggatt cgcgaactgt tcgagatgaa aagcggtatc ggtacgttct
     4801 ttgccgagaa agtcttcact gaaagcttcg taattgacgg ggtcggcaat catggcggca
     4861 atttgtgcgg cagtatcgtt gatacgcgtc ctatcttgct cccagtctga gaaactgtgg
     4921 cgcagacttt ctatggtggg aaatttcttc attagccact cgaggtaatt atagccgttt
     4981 ggtgggaagg taccgacagc gaagtggaag gtttcacagc cgagcgggat aggtctgtgc
     5041 caccaaccgc gtgggatgta gaggacatca cctgcttcaa ggataatatc catatcgata
     5101 tgttcaggaa tggaaatatc agtatcttta gtctgttgca tatacaatgg catagggaaa
     5161 tcaggggcag taagttgcca acgtttcttg ccgaaaagct ggacggcata catatctcgg
     5221 gcgtcccaat ggtttttata agattcgtcg ctgccaaaag caagatatcc actaacaata
     5281 gtatgtgcgc cggcaaagcg ggcgacttga cgggcgatat ggtctgaaaa cggctcgttg
     5341 ttaatatggt tatagactaa cgacgcaccg ttcttcatat gttcgtagat aacggattta
     5401 ataaaacggt agcgagtttt gcccaaatcg tcgaaacttt cgacgtattc ttctttagga
     5461 acgattgcgc cttttttacg cagatgaaac agcggtgcgg ttgggtctgc tcgttggtat
     5521 atctcgttga tatatttaca agatgcggct tcgagattcc gaaccgctcc tttaaagagc
     5581 ttgggctttt gatacagata agtctgtcgg aacgttttag gactaatgcc gaagtcgaga
     5641 tggatgccca ttacttcccc ttactcagaa aatatttaaa atttataatg ttacatatag
     5701 ttacaaatat tagagttttt tgtgtgtgcg tcaaggaatt gttgacaatt ttagttaaaa
     5761 atttgtctcc aaacggaaaa aagcggtttg ttttttgttg ttaacatttt tattgtaatt
     5821 aaataaaagg tcgtctgaaa acggttttca gacgaccttt tgctataatc gggcttcatc
     5881 gccccgttcg gtttggaacc ttatgaaaac cctcgtcctc ctcctgcttt ccttctccac
     5941 gaccaccgct ttcgccgcat acggtttggg tttggggcag gcaccgaaat atcctgccgg
     6001 ctttcgcgcc tacggttatg tttattccgg acggcagggc tgggttttaa aaacagaggc
     6061 ggatgccatt aaattagaca cgcttttcaa acgctttgtg taccgtcctt ccgccgccaa
     6121 tcaaaacccc gtcggacagc gttcggacgg catacccgcc aaccacacaa aggaaaaacc
     6181 atgagcaaaa aaatcaaagc cggcattgtc ggcgcgacgg gctacacggg cgtggaactg
     6241 ctgcgcctgc ttgccgccca tccagatgtc gaagtcgccg ccgtaaccag ccgcagcgaa
     6301 gcgggaaccg cagttgccga ttactttcca agtctgcgcg gcgtgtacgg cctcgccttc
     6361 caaacgcccg acgaggcagg tttggaacaa tgcgacatcg tcttcttcgc cacgcccaac
     6421 ggcatcgcca tgaaagacgc gccgcgcctg attgaacagg gcgtgcgcgt catcgacctt
     6481 tccgccgact tccgcatacg ggacattccg acctgggaac actggtacgg catgacccac
     6541 gccgcccccg gcctcgtttc ccaagccgtg tacggattga gcgaactcaa ccgcgaagcc
     6601 gtcgcacagg cgcgcctcgt cgccaacccc ggctgctacc cgacctgcgt atccctgccg
     6661 ctcgtgccgc tgttgcggca atgccgtctg aagcccggta tgccgctgat tgccgactgc
     6721 aaatccggcg tgtccggcgc gggcaggaaa ggcaatgtcg gttcgctgtt gtgcgaagcc
     6781 ggcgacaact tcaaagccta cggcacagcc ggacaccgcc acctgcccga aatcaggcag
     6841 accatcgccg ggcttcagga cggcatcgcc gaaggattcg tgttcacgcc gcacctcgcg
     6901 ccgatgatac gcggtatgca cgccaccgtt tacctccacc tttcagacgg cagcgacccc
     6961 gaaaccgtcc tgcgcgacta ctaccgcgac agcccgttca tggacatcct gcccgccggt
     7021 tcgacacccg aaacccgcag cgtgcgcggc gcaaacctct gccgcatcag catccgtcag
     7081 gcggcgcaat ccgatgtgtg ggtcgtcctt tccgtcatcg acaacctcgt caaaggcgcg
     7141 gcgggacagg cggtgcaaaa tatgaacatt atgttcggac tggaggaaac acacggcttg
     7201 ggcggcatcc ccctgctccc ctgaagcgta aacggcaaac cgcaggcatc gtgcctgcgg
     7261 ttttttgatg ccgtctgaaa gcggcggttt ttttgggttc ggacggcttt tggctcatca
     7321 ttccgcgaaa acaaaaatct aaaataccgt cattcccgca aaagcgggaa tctagtttat
     7381 ccggcttcag caatttccga cacatttcca cacgcttcga ttccgtcatt tctccggttt
     7441 caatcgtttc cgataacacc gtggtttttc atttctagat tcccgcctgc gcgggaatga
     7501 cggcgggggg cttgccgttt tcccgataag cctctgcggc ttctcattgc cggatttccg
     7561 cccgcttggg aataacggga tttaaaatca tgaaaatgtg tcaaaaataa tatag
//
LOCUS       DAXDXW010000087         7518 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXDXW010000087 DAXDXW010000000
VERSION     DAXDXW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.088"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            185..334
                     /locus_tag="ACFB64_001854"
     CDS             185..334
                     /locus_tag="ACFB64_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210158.1"
                     /translation="MNAEGFCEKQTACRPDGRRNAGDRADLKAFEFAGRELANYREML
                     KRYAV"
     gene            364..3411
                     /locus_tag="ACFB64_001855"
     CDS             364..3411
                     /locus_tag="ACFB64_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFC1210159.1"
                     /translation="MSDLEAKVRITVENHTKQGFDSAAADADKAAEKMRSSGDNAAKG
                     FKAAIDSMHETMRNLHADVKAGFEAAGNQAQQASEKVRAEVGKIGSGLSGLTKLLAGL
                     ATADFAKSVLDTADAMQSINSQVRQVTSSETEYLAVQQQLLDTANRTCASLESTANLY
                     VSTSRALKDYGYTQQEILKFTEATNNAMTIGGVGARQQAAALFQLSQALGSGVLQGDE
                     FKSISESAPILLDTIAEYMGKSRDEIKKLGSEGKLTADVIFKAISGASEKFGEQAAKM
                     PVTMGQALTVFSNNWQSMVSKLLNDSGTMSGIAAVIKLIADNLNLVVPIVAGFAVAVA
                     AAVAPTLALNLALLANPFGIIAVAIGTVVGLIAKFGDEIDVFGGGWSNLSDVIRAVWQ
                     IITETVGEAVGTVKSWFDGLTGRLNEGAGGWPSLFGRVMSVISSAIGAYVNVYINIFA
                     TGWMLIKEAANDMPQFFANLGKAIGNVFISAIERMINKAVGMINSMIDFANKAASMAG
                     ISGIGKLNKVRMGRMDDGGLGERIHDSLTKDRAGAMANAVRERAADIHEADALKGRGG
                     GGHAKTARKKPGANQGGGKGGKSRSGGPGAAKDPMQAWEGEIKARKLAHREMQRETLA
                     HQEWDLAREAEYWRAKLATVDANGKTGVKIREKILTLEDQLSKQSTEAKMNQAAEWEK
                     LDKHKLEMEKDAADQALADGRISQLERLDLEIEFENRRYQIAYDALQERIALAEQDPA
                     YSQAAIGKLKAQMGELGRGHERTQAKNEGKRENQRRKDAPNVMEMLQDGGRNVWQEAQ
                     QQMGQAFTAMLARAQNFRQAMNGFFKSMGQTFIQEMVTKPLTGLMRRMVEESAIYKMI
                     FGTKETLETAAAAKTAATKATETTAVVSSNAVQAASGAAASQAHIPYVGPILAVAAMA
                     AMMSAVMGLMGGGGGSQTTTTTTRIPSAAGGWDIPAGINPLTQLHENEMVLPAEHAQT
                     IREMADQQGGGSTVIINSTGGDFIHKNDLAKLLKQMKRDFKFV"
     gene            complement(3471..3950)
                     /locus_tag="ACFB64_001856"
     CDS             complement(3471..3950)
                     /locus_tag="ACFB64_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFC1210160.1"
                     /translation="MTDNLIQIATPILTVIGVFVAAYGIMRNTENAKKRATIDMIMAE
                     RNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAILTVLNQREFVCAGVLGG
                     ALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQEFEAVARKWKKKPLKTK"
     gene            complement(4292..5281)
                     /locus_tag="ACFB64_001857"
     CDS             complement(4292..5281)
                     /locus_tag="ACFB64_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC1210161.1"
                     /translation="MATITQRNGKWRVQIRMKGVSRSATFERASDAKAWAARIESQIM
                     DGIQGNAPRNTIFADLIRRYLSEVTPSKRGAREESYRIGRALKTPLAKVRLADLRPQD
                     FADWRDQRLQEVSPTSVGRELTTLSAVCEHAMKEWGLLRENPVRKISKPKKSRARTRR
                     PTEQEIADICAALLYRPNEKPKMAVQRVAVAVLFAIETAMRAGEICGLKWADVNMRRR
                     IAHLPITKNGDSRDVPLSLRAAELIEQLRGIDDTWVFSLDAKSLDVLFRRARDNCGIQ
                     GLHFHDTRREALTRLSKKVPVEVLAKISGHRDLRILLNVYYRPDMADIAKMLD"
     gene            complement(5541..5729)
                     /locus_tag="ACFB64_001858"
     CDS             complement(5541..5729)
                     /locus_tag="ACFB64_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210162.1"
                     /translation="MADGRKGFFDAIIKINIFFFPVKTPTLGAANPIAGGSTRFRFGA
                     EQMAVLYVPFCVLKHIGR"
     gene            5970..7004
                     /gene="purM"
                     /locus_tag="ACFB64_001859"
     CDS             5970..7004
                     /gene="purM"
                     /locus_tag="ACFB64_001859"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC1210163.1"
                     /translation="MSTSLSYRDAGVGIDAGDQLVEKIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPKNTVAQIDAESWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVRSFLSGQGETVYRLGCIRER
                     QGNEHQTQVA"
BASE COUNT         1898 a         1837 c         2065 g         1718 t
ORIGIN      
        1 aggcgcacca ataatgggcg gaaatgaaaa tgccgtaccg atccggacaa caaccgatgc
       61 cgcaccctgc gggcaggctt cgcactctga aaggctttcg gacggcattg ttcttacctg
      121 cgcgtcaaac cgccttcgtg taaagcggtt gcaaaaaagc cttttatttt gacagggtgc
      181 agagatgaac gccgaaggat tttgtgaaaa acaaaccgcc tgccggccgg acgggcgccg
      241 aaatgcgggc gatcgtgccg atttgaaggc ctttgagttt gccggacggg aattggcgaa
      301 ttatcgggaa atgttgaaac gttatgccgt ctgaaaagac ggctttttta tttggggctg
      361 taaatgagcg atttggaagc caaagtcagg ataacggtag aaaaccatac gaagcagggt
      421 tttgattcgg ccgccgccga tgctgataag gcggcggaaa agatgcgcag cagcggcgat
      481 aatgccgcaa agggctttaa agctgcaatc gacagtatgc acgagaccat gcgcaatttg
      541 cacgccgatg tgaaagcggg atttgaagcg gcgggcaatc aggcgcaaca ggcgtctgaa
      601 aaggttaggg ccgaagtggg taaaatcggc tcaggcttat cggggctgac caaactgctg
      661 gcaggattag caaccgcaga cttcgccaaa tcggtgcttg ataccgccga tgcgatgcag
      721 tcgataaaca gccaagtccg acaagtcacg tcgtctgaaa cggagtatct ggccgtgcag
      781 cagcagcttc tcgatacggc aaaccgtacc tgtgcatcgc ttgaatcaac ggcaaacctg
      841 tacgtttcca caagccgcgc gttgaaagac tacggctaca cgcagcagga aattttaaaa
      901 ttcaccgagg cgaccaacaa cgcgatgacc atcggcggcg ttggcgcgcg acaacaggcc
      961 gccgcgctgt tccagttatc gcaggctttg ggcagcggcg tattgcaggg cgatgaattt
     1021 aaatccattt ccgaatccgc acctattttg ctggatacca ttgcggaata tatgggcaaa
     1081 tcccgcgacg aaatcaaaaa gctgggcagc gaagggaaat tgacggcgga tgtgattttc
     1141 aaagccattt ccggcgcgtc ggagaagttc ggggagcagg cggccaaaat gcccgtgacg
     1201 atggggcagg ctttgacggt gttctcgaac aactggcaaa gcatggtttc aaaactgctg
     1261 aacgacagcg gcacaatgtc ggggattgcc gccgttatta aactgattgc cgataacctg
     1321 aatttggtcg tccctatcgt cgcaggtttt gccgttgccg ttgccgccgc cgttgcaccg
     1381 acactggctt tgaatcttgc actgctggca aacccgttcg ggattattgc cgtcgcaatc
     1441 ggcacggttg tcgggctgat tgcgaaattc ggcgatgaaa tcgatgtttt cggcggcggc
     1501 tggtcgaatc tttccgatgt gattcgggcg gtttggcaaa tcatcacgga aaccgtcggg
     1561 gaagcggtgg gaaccgtcaa atcgtggttt gacgggctga caggccggct gaacgagggc
     1621 gcgggcggct ggccgtcgtt gttcgggcgc gtgatgagcg tcatctcaag cgcaatcggc
     1681 gcgtatgtga acgtttatat caacatattc gcaaccggct ggatgctgat taaagaagcc
     1741 gcaaacgata tgccgcaatt cttcgccaat ctcggcaagg ccataggcaa cgtgtttatt
     1801 tccgcgatcg aacggatgat aaacaaagcg gtaggcatga ttaacagcat gattgacttt
     1861 gccaacaaag ccgcgtcgat ggccgggata tcgggcattg gaaagctgaa caaggttcgg
     1921 atggggcgga tggacgacgg cgggcttggc gagcgtattc atgacagctt gacgaaagac
     1981 cgcgccggcg caatggcaaa tgccgtccgc gaacgggccg ccgatattca cgaagccgat
     2041 gccctcaagg gacgcggcgg cggaggacac gccaaaaccg cccggaaaaa gccgggcgca
     2101 aatcagggcg gcggcaaagg gggcaaatcc cgttcgggcg gtccgggcgc ggccaaagac
     2161 ccgatgcagg cttgggaagg ggaaatcaaa gcccgaaaac tcgcacaccg cgaaatgcag
     2221 cgcgaaacgc tcgcacacca agaatgggat ttggcgcgtg aggcggaata ttggcgggcg
     2281 aagctggcaa cggtggacgc caacggcaaa acgggcgtga aaatccgcga aaaaatcctg
     2341 acgcttgaag accaattatc gaagcagtca accgaagcca aaatgaatca ggcggccgaa
     2401 tgggagaaat tggacaagca caagctggag atggagaaag acgcggcaga ccaagccctg
     2461 gcagacggac gtatttcgca actcgaacgc ctggacttgg aaatcgagtt tgaaaaccgc
     2521 cgttaccaaa tcgcctatga cgcattgcag gaacgcatcg cacttgccga acaagacccg
     2581 gcatacagtc aggcggcaat cggcaagctc aaggcccaaa tgggcgaatt ggggcggggg
     2641 cacgaacgga cgcaggcgaa gaacgagggc aaacgcgaaa accaacgccg caaagacgcg
     2701 cccaacgtca tggaaatgct gcaagacggc ggcaggaacg tttggcagga agcacagcag
     2761 cagatggggc aggcgttcac cgccatgctg gcgagggcgc agaacttcag gcaggcgatg
     2821 aacggctttt tcaagagtat ggggcaaacc tttattcagg aaatggttac gaaacctttg
     2881 acgggcttga tgcggcgcat ggttgaggaa tcggccattt acaagatgat tttcggaact
     2941 aaggaaacgc tggagacagc ggcggcagcc aagacggcgg caactaaggc aaccgagaca
     3001 acggcggttg tcagtagtaa tgctgttcaa gcggcttcag gcgcggcggc ttcccaggcg
     3061 catatcccat atgtcggccc gattcttgcc gttgcggcta tggcggcgat gatgtcggca
     3121 gtgatggggt tgatgggcgg cggcggcggt tctcaaacga ccacaaccac gacgcgcatt
     3181 ccctcggcgg caggcggctg ggacatcccg gcaggcatca acccgctgac ccaactgcac
     3241 gaaaacgaga tggtcttacc cgcagaacac gcccaaacca tccgtgaaat ggcggatcag
     3301 cagggaggcg gcagcaccgt catcatcaat tcgacaggag gcgacttcat ccataaaaac
     3361 gatttggcga agctcttgaa gcagatgaaa cgggacttta aatttgtctg agaaaatgcc
     3421 gtccgaaagg ttcggacggc ctatgttgcg gcgtacgtca agctattaag ctatttggtt
     3481 ttcagaggct ttttcttcca tttccgggct acggcttcaa attcttgaaa ggccgtcggc
     3541 gcgctcctga tacggcgtat ttcaaaaatg aagctgctta ggttgtccca gtcgcgtaac
     3601 agcatggagt attcgaaatc tttatacatt ttctcgtgca gcgccccgcc caatacgccc
     3661 gcacagacaa attcgcgctg gtttaaaact gtcagtatgg cttcacggtc tttcttcttg
     3721 tccggggtat ctgatgtata ggtggcgagt atgcatccgt ctgtttttgc cagcccgttt
     3781 actatggtta tggcttcttg aagggcggca ttgttacgtt cggccatgat catgtcgatg
     3841 gtggcgcgct ttttggcgtt ttccgtattc ctcatgatgc cgtaagcggc aacaaaaacg
     3901 ccgataacag tcaatatcgg cgttgctatc tgtattaggt tgtcagtcat tgccgtccca
     3961 gccgtccgaa aggcggaaat cacggctttc cgaataaata tgccccattg tcatcccctt
     4021 aagctgatgt tgagaataaa tccttgcgtc ggaatatacg gcattgcctg actattttca
     4081 agattccgca ccccgcggac cggactatac aaaaaaaccg ccttcgtgta aagcggttga
     4141 agagagcctt tcaataaaaa tgccgtccga agggctttcg gacggcattt tttatagatt
     4201 cttttttaat gaacctttga aaccgaagat aattaatttt cctctttatt ttcaatacgc
     4261 agtgtgccct atgtttttaa ataaaaatat tttaatccaa catttttgca atatccgcca
     4321 tgtcggggcg gtagtaaaca ttgagtaaaa tccttaaatc ccgatgcccg ctgatttttg
     4381 ccaagacctc cacaggcact ttttttgata ggcgcgtgag ggcttcgcgg cgggtgtcgt
     4441 gaaaatgcag gccttgtatg ccgcaattat cccgcgctcg cctgaataat acatcaaggc
     4501 ttttggcatc caggctaaac acccatgtgt catcaattcc gcgcagttgc tctattagtt
     4561 cggcggctcg caaggataat ggcacgtcac ggctgtcgcc gtttttagtt attgggaggt
     4621 gggcaatacg acgccgcata ttcacatctg cccattttag gccgcagatt tcgcctgccc
     4681 gcatggcggt ttcgatggca aataggacgg caacggcaac ccgttgcacc gccattttcg
     4741 gtttctcatt aggccggtat aggagggcgg cgcaaatatc ggcaatttcc tgctcggttg
     4801 gccgtcttgt ccttgcccgg ctttttttcg gcttgctgat tttgcgtaca gggttttcgc
     4861 gaagaagtcc ccactctttc atggcgtgtt cgcagacggc ggataaagtg gttaattcgc
     4921 gtccgacgct ggtgggggat acctcctgca accgttgatc tcgccaatcg gcgaagtctt
     4981 gagggcgcag gtcggcaagc cgcacctttg ccaaaggtgt ttttagtgcg cgtccaatgc
     5041 ggtatgattc ttcccgtgcg ccccgctttg atggtgtaac ctcggataaa taccgccgga
     5101 taaggtcggc aaagatagta tttcgcgggg cattgccttg gatgccgtcc ataatttgcg
     5161 actctattcg cgccgcccac gccttcgcgt ctgatgcccg ctcaaatgtg gcggaacggg
     5221 aaacgccttt catacggatt tgtacgcgcc atttgccgtt acgttgagtg atggttgcca
     5281 tatttattcg ggtgcaattc gggtgcaaat ataatagagc aaaacagata taaagcgggg
     5341 caaaataggg ctatattaaa tttaatgctt tgatatgtag ggataaatag atttaaaaag
     5401 acgaaagaag tgcggacgta gaataagcgt agatgcgctc cgtccgcacc aataaaaata
     5461 tataaatcat attgttaatg attattggga caattcggtg gcacaaaata taagctgtct
     5521 gcttttcagc gggcggcttt ttatctgcct atgtgtttca acacacaaaa cggcacataa
     5581 agcaccgcca tttgttccgc accgaatcgg aagcgggtgc ttccgcccgc aatcggattc
     5641 gccgccccta atgtcggggt tttaaccgga aagaagaaaa tattgatttt gatgatggca
     5701 tcaaaaaaac ccttccgccc gtctgccaaa aggaatggtt aagaaaagca atgcgtcttt
     5761 tgatggaacg cgatatataa gaaattgagg ggaagggcag gcgggtttct gcccgttaat
     5821 cctgcggacg gccccgccaa acccgacacg cgcgggcata tggcggattg tttacaatat
     5881 tgtctaaaaa tctgaacctg caaacgcgaa ataaggtaga atacgccccg ttattttacc
     5941 gtccaatccc catttaccaa ggaaaaacga tgagtacttc attgagttac cgcgatgcgg
     6001 gcgtcggtat cgacgcaggc gaccaactgg tcgaaaaaat caaaccgttt gccaaacgca
     6061 ccatgcgtcc ggaagtattg ggggatttgg gcggttttgg cgcattggtc gaaatcggca
     6121 aaaaatatca aaaccccgta ttggtcagcg gtacggacgg cgtgggtacc aagctcaaac
     6181 ttgccttcga ttgggacaaa cacgatacgg tgggcatcga ccttgttgca atgagtgtca
     6241 acgatatttt ggttcaagga gctgaaccat tgtttttctt ggattatttt gcctgcggta
     6301 aattggatgt tccgcgtgcg accgatgtca tcaaaggcat tgcccaaggt tgcgaagaat
     6361 ccggctgcgc cctgattggc ggggaaactg ccgaaatgcc gggtatgtat cccgttggtg
     6421 aatacgattt ggcgggtttt gccgtcggcg tggtggaaaa agagaatgtc attaccggcc
     6481 gcagcatcgg cgcgggcgat gtggtattgg gtttggcttc caacggcgca cattcaaacg
     6541 gctattccct tatccgtaaa atcatcgaac gcgacaatcc cgatctggat gccgagtttg
     6601 ataatggcaa aactttacgt gaagccgtta ttgcgccgac acgtctgtat gtgaaaccga
     6661 ttcttgccgc tttggaaaaa tttaccatta aaggtatggc gcacattacc ggcggcggca
     6721 ttaccgaaaa cgtgccgcgc gtgttgccta aaaacacggt tgcccaaatc gatgccgaat
     6781 cgtgggaatt gcccaagctc ttccaatggc ttcaaaaggc gggcaatgtg gaaacccaag
     6841 aaatgtaccg gacctttaac tgcggcatcg gtatggtcgt catcgttgct gccgaagatg
     6901 ccgatgcggt tcggtcattc ctgagcgggc agggcgagac agtgtaccgt ttgggttgca
     6961 tccgagagcg tcaagggaac gagcatcaaa cccaagttgc ctgacttcca agcatcaaat
     7021 gccgtctgaa tttgtttcag acggcatttc cgcatcttcc aaacgcgtat ccgcaacaca
     7081 gttctgcaaa ctgatatttt gcccggtatg gcggcttaaa cttcttcatc atatttcgat
     7141 aatgctataa aatgatataa tcataaaata ttgtttattc ctacatcgta ttcatcctat
     7201 tttccgaaat gtgagcgttt tatgaagcaa tccgtccata ataaaacaat tttaagtgtt
     7261 ttgatttcat ctatgtctgt aacggctttt tccgcccccc accccccccg cgataaatgc
     7321 tatttcagtc aatcgtaccg attccggcga actcatcggt tttgagttta ataatgcgga
     7381 caataccgta accggcaatg cggatattac ggtcaaacct gccgaccctt cacagcattc
     7441 gcgcccgaaa gggctgatta tccggagcga ccgtaacgaa ccaaacaaaa accaagaagt
     7501 tcgcttaaat aatatagc
//
LOCUS       DAXDXW010000088         7469 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXDXW010000088 DAXDXW010000000
VERSION     DAXDXW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.089"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(12..428)
                     /gene="dksA"
                     /locus_tag="ACFB64_001860"
     CDS             complement(12..428)
                     /gene="dksA"
                     /locus_tag="ACFB64_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC1210164.1"
                     /translation="MVKLTEQDILNWIGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     VTTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVEAQERRERMKKQFAD"
     gene            complement(576..1385)
                     /gene="proC"
                     /locus_tag="ACFB64_001861"
     CDS             complement(576..1385)
                     /gene="proC"
                     /locus_tag="ACFB64_001861"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC1210165.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGCRIHIANRGAEKRE
                     RLGKELGVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVGTL
                     SRYLGGSRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDE
                     AQMHSITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVCRSQEMERQYQ"
     gene            complement(1416..1823)
                     /locus_tag="ACFB64_001862"
     CDS             complement(1416..1823)
                     /locus_tag="ACFB64_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFC1210166.1"
                     /translation="MEIRVIKYTATAALFAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPLSLEDYEIPLSDGNRSVRANEYESAQKSYFYRKIGKFEACGLDWRTR
                     DGKPLVERFKQEGFDCLEKQGLRRNGLSERVRW"
     gene            complement(1823..2509)
                     /locus_tag="ACFB64_001863"
     CDS             complement(1823..2509)
                     /locus_tag="ACFB64_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC1210167.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGREPHSVGLIAVSKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSRQRRSLMPPLQVCVEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2629..3672
                     /gene="pilT"
                     /locus_tag="ACFB64_001864"
     CDS             2629..3672
                     /gene="pilT"
                     /locus_tag="ACFB64_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC1210168.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMARSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEA
                     ARRRAQNSESMSF"
     gene            3836..5062
                     /locus_tag="ACFB64_001865"
     CDS             3836..5062
                     /locus_tag="ACFB64_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC1210169.1"
                     /translation="MNTDNLHDILDETVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5179..7329
                     /gene="yccS"
                     /locus_tag="ACFB64_001866"
     CDS             5179..7329
                     /gene="yccS"
                     /locus_tag="ACFB64_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC1210170.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYEALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIRRLLEMQ
                     GQACRNTAQAIRSGKDYTYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLSRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSFKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVYQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIAGTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSSGTYLQKIAERLKTGETGDDIE
                     YRITRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPDMGPDDFQTALDTLRGELGTLRTRSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1803 a         2522 c         1655 g         1489 t
ORIGIN      
        1 gtttgcctcc atcagtcggc aaactgtttc ttcatcctct ctcggcgttc ttgggcctca
       61 acggataaag tggctgtcgg gcgtgccagc agccgcttca gaccgatagg ttctcccgta
      121 tcggcacaga atccatagtc cccttcatca atattgcgga tggtcgcctg tattttactg
      181 agaagttttc gttcccgatc ccgggtacgg agttccaatg cgtactcttc ttcctgtgtg
      241 gcacggtcgg caggatcggg ggctgattcg tgttcttgga gatgccctgt tgtaacggag
      301 gcgttttcga tgagttcgtc ttgcattttt accagcaatt cgcggaaaaa agccaaatgg
      361 tcgtcattca tataatcgtc ttccggccca atccaattca aaatatcttg ttctgtcagc
      421 tttaccataa tgtatgccct ctcggttcac cgcaaaaaaa caaagcacta caaagtggct
      481 ttattccttt gatttttata aggcttattg cctaaggcgt ttttgaaaac gcgcatcata
      541 acacgttttg gattggtttt tttattttct ttacattatt gatattgccg ttccatttcc
      601 tgcgaacggc acacacaggc acaaacgccc tcacttatgg cttcggcgac acgatgccgt
      661 ctgaaggctt ccacggcttc gtgggttgtc ccgccttttg acgttacatt tttttgaagc
      721 ttctcgaaat cttcacccgt ctgctcggca agggcaaccg ctcctttaaa cgttgccaga
      781 ctgagcgcgc gtgcttctgc catatcaaac ccttgtcgga tggcggcatt ttgcaatgcg
      841 tccagcagat aaaacacata agccggcccg ctgccgctga tgccggtaat gctgtgcatt
      901 tgcgcttcat cttccaacca aacggtcaaa ccgactgatt tcatgattcg atcggcaatc
      961 ctgcggtctg tttccgatac ttccgcttcg gcatacatac cggatacgcc cagcccgatt
     1021 tttccgggtg tattcggcat aacccggaca atgcggcggc ttcccccgag gtaacggctg
     1081 agcgtaccga ccgacaatcc ggctgcgaca gaaagcacca atgcaccgtt ggtgcggata
     1141 tttttgcacg cggcttccat atcctgcggt ttgacggcaa ggattaaaac atcgtcggaa
     1201 tgaagctccg gcagggttgc cgaagtttcg acccccaact cttttcccaa acgttcgcgc
     1261 ttttccgcac cacggttggc tatatggatg cggcatcccc cttgtttgac caatccgccc
     1321 gcaacggcag ccgccatatt tccgccgccg agaaaataaa cattcattat ttctacccta
     1381 ttcaaaatta ccttactaaa ttcattccca attttttacc atcggacgcg ctcggacagg
     1441 ccgttgcgcc gcaacccctg cttttccaaa cagtcgaaac cttcctgttt gaacctctca
     1501 accaaaggtt tgccgtcacg cgtacgccaa tccaacccgc aggcttcaaa cttccctatt
     1561 ttcctataaa agtaagattt ttgcgcggat tcatattcgt ttgccctgac ggaacgattg
     1621 ccgtctgaaa gcggtatctc gtagtcctct aaagacagcg gactctcgcc gccaatatcc
     1681 caaaaatcga tggcggcagg ttttctcggc ttacaccagc cggacaagga cgaacactca
     1741 taccaccccg ccagccggca gcctgcaacc gtaaatgcaa acaacgcagc cgttgccgta
     1801 tattttatta cccgaatttc catcagcccc ttttcccgaa aatcgcgctg ccgatacgga
     1861 cgtgtgtcgc accgcactca atggcggcag gcatatcgtc cgacatcccc atagacagca
     1921 cgtccgcctt aacgccagcc gcattgaggt cggcaagcag cttttgcatg gtctgaaact
     1981 gagttttcaa ctccgtttca ctgctgttgg ctttggcaac gcacatcagt ccgcgcacga
     2041 caaggttggg cagcttcgcc acttccacgg caagcgcgac cgcttcttcg ggcgcgacac
     2101 cgtgttttgc cgcctcgccc gcaatgttca cctcgacaca cacctgcaag ggcggcatta
     2161 aggagcgacg ctgcctgctc agccggacgg cggtcttcag acggcatacg gtatgcaccc
     2221 aatgcgcgcg ttcggcgaca aatttggttt tgttggactg cacatcgccg atgacgtgcc
     2281 acacgatgtc ggtcaaatcc gccaattcct ccgttttgcc gtaccactcc tgaatatagt
     2341 tctcgccgaa atcacgctgt ccggcagcgt aaacttcgcg gatgccgtct gaagggaagg
     2401 tcttgctgac ggcaatcagg ccgacggaat gcggctccct gcccgcctgc agaaccaatt
     2461 ttccgatacg gtcggacacc tcacgataac gttcttgcaa caccgtcata gatcatcccc
     2521 taattaaaaa tgattaaaca gttgaaaccc ctccagtcag ggcggtataa tcaaggttgt
     2581 tagaatcatt ccaaccaatc gaaacattat actaaacaga gccgcattat gcagattacc
     2641 gacttactcg ccttcggcgc taaaaacaaa gcatccgacc ttcacctgag ttcgggcata
     2701 tcccctatga ttcgggttca cggcgacatg cggcgcatca accttcccga aatgagcgcg
     2761 gaagaggtcg gcaatatggt aacttcggtg atgaacgacc accagcggaa aatctaccag
     2821 caaaacttgg aagtcgactt ctcgttcgaa ctgcccaacg tcgcccgatt ccgcgtcaac
     2881 gccttcaaca ccggccgcgg ccccgccgcc gtattccgca ccattcccag caccgtctta
     2941 tcgctggaag aattgaaagc cccgagcatt ttccaaaaaa tcgcagaatc gccgcgcggc
     3001 atggtattgg ttaccggccc taccggttcg ggcaaatcga ccacgcttgc cgcgatgatc
     3061 aactacatca acgaaaccca gccggcacac atcctgacca tcgaagaccc gatcgaattc
     3121 gtccaccaaa gcaaaaaatc cctgattaac caacgcgagc tgcaccagca caccctcagc
     3181 ttcgccaacg cgctgagttc cgcattgcgc gaagaccccg acgttatcct tgtcggcgag
     3241 atgcgcgacc ccgaaaccat cggcttggca ctgaccgccg ccgaaaccgg acacttggtt
     3301 ttcggcacgc tgcacacgac cggcgcggca aaaaccgtcg accgtatcgt ggacgtattc
     3361 cccgccggag agaaagaaat ggcgcgttcc atgctgtccg aatcgctgac cgccgtcatc
     3421 tcccaaaacc tgctgaaaac gcacgacggc aacggccgtg tcgcctcgca cgaaatcctg
     3481 attgccaacc ccgccgtccg caacctcatc cgcgaaaaca aaatcacgca gattaactcc
     3541 gtcctgcaaa ccgggcaggc gagcggtatg cagacgatgg accaatcgct gcaatcgctg
     3601 gtgcgccaag ggctgatcgc accggaagcc gcacgcagac gcgcgcaaaa cagcgaaagt
     3661 atgagtttct gacacacaac cgccttccgg ccatgccggc gggaaaacaa ggcgcaaaca
     3721 cgcggggcgg gacgcagcat cccgcccggc atacccttcc gaacaggaag cgtccgcctt
     3781 cctgttgaaa cctgccgccg caaactgcaa ggcttaaacc gaaaagaagc taacgatgaa
     3841 taccgataac ctgcacgaca tcttggacga aacggttcaa gtgtattctc aaaaaaaaca
     3901 aagccgatcc gaaaccccgg ccgaaatcgg cgcacacttc cacccgctgc tcgaccgcct
     3961 gtgcgaaacc gcagaagcac aaaacgcgtc cgacatcctt atcagcaaag gattcccgcc
     4021 ctcgttgaaa atcaacagcg cattaacccc gcagccgcaa aaggctctga cgggcgagga
     4081 aaccgccgcc atcgccgcat cgacgatgaa cgccgaacaa tcggaaatat tccggcgcga
     4141 cggcgaaatc aactactccg tccaatcgcg cagcggcacg cgctaccgcg ccaacgccta
     4201 ctacagccaa ggcagcgcag gtttggtttt gcggcgcatc aaccacgtca tcccgcaaat
     4261 gcgggaattg ggcttgcccg aaaaactcaa agacctcgcc gtcgcaccgc gcgggctgct
     4321 gattatcgtc gggcctaccg gttcgggcaa atccaccacg atggcgacta tgctcgaaca
     4381 ccgcaacaaa accctgcccg gccatatcgt taccatcgaa gacccgattg agtttatcta
     4441 caaaccgcgc cgctgcatct ttacccagcg cgaaatcggc gtcgacacca taaactggca
     4501 gacggcggta caaaacgcta tgcgccaatc cccagacgtg gtctgtatcg gcgaagtccg
     4561 cagcagggaa agtatggaat acgcgatgca gctcgcccaa accggccacc tgtgcatttt
     4621 tacgctccac gccaacaccg cgccgcagtc gctcgaacgc atactcaact tctaccccaa
     4681 agaacagcac aaccaaatac tgattgacat cgccctcaat ctgaccggca tcatctgcca
     4741 acgcctcgcc ctcaaaaaag acaaaacggg caggacggcg gttgtcgact tgctcatcaa
     4801 cacgcccgcc atccaagact tcatcctgaa gggcgacctg atgaacatca gtaaaatcat
     4861 ggaaaccgcc aaaaccgacg gaatgcagac gatggaccaa aaccttttcg aactgtaccg
     4921 tcacggcatc atcagttacg aagaagccct gcgccagtcc gtttccgcca acaacctgcg
     4981 attgcacatc caactgcaca aagaaggcaa aacgcccgaa ctcctttacg acagggtcaa
     5041 cggtctcaac ctcatttcct gatccgcaaa acccaatgcc gtctgaaaac cgcatccccg
     5101 ttttcagacg gcatgatttt atccgcccca ttcatgtgcc acactttatt cacttcataa
     5161 caataaaccg gtaaaaccat gaaaacccca ctcctcaagc ctctgctcat tacctcgctt
     5221 cccgttttcg ccagtgtctt taccgccgcc tccatcgtct ggcagctagg cgaacccaag
     5281 ctcgccatgc ccttcgtact cggcatcatc gccggcggcc tggtcgattt ggacaaccgc
     5341 ctgaccggac ggctgaaaaa catcatcgcc accgtcgccc tgtttaccct ctcctcgctc
     5401 acggcgcaaa gcaccctcgg cacagggctg cccttcatcc tcgccatgac cctgatgacc
     5461 ttcggcttta ccattttagg cgcggtcggg ctgaaatacc gcaccttcgc cttcggcgca
     5521 ctcgccgtcg ccacctacac cacacttacc tacacccccg aaacctactg gctgaccaac
     5581 cccttcatga ttttatgcgg caccgtactg tacagcaccg ccatcatcct gttccaaatc
     5641 atcctgcccc accgccccgt ccaagaaagc gtcgccaacg cctacgaagc actcggcggc
     5701 tacctcgaag ccaaagccga cttcttcgac cccgatgagg cagcctggat aggcaaccgc
     5761 cacatcgacc tcgccatgag caacaccggc gtcatcaccg ccttcaacca atgccgttcc
     5821 gccctgtttt accgtttgcg cggcaaacac cgccacccgc gcaccgccaa aatgctgcgc
     5881 tactacttcg ccgcccaaga catccacgaa cgcatcagct ccgcccacgt cgactaccaa
     5941 gagatgtccg aaaaattcaa aaacaccgac atcatcttcc gcatccgccg cctgctcgaa
     6001 atgcaggggc aggcgtgccg caacaccgcc caagccatcc ggtcgggcaa agactacaca
     6061 tacagcaaac gcctcggacg cgccatcgaa ggctgccgcc aatcgctgcg cctcctttca
     6121 gacggcaacg acagtcccga catccgccac ctgagccgcc ttctcgacaa cctcggcagc
     6181 gtcgaccagc agttccgcca actccgacac agcgactccc ccgccgaaaa cgaccgcatg
     6241 ggcgacaccc gcatcgccgc cctcgaaacc ggcagcttca aaaacacctg gcaggcaatc
     6301 cgtccgcagc tgaacctcga atcaggcgta ttccgccatg ccgtccgcct gtccctcgtc
     6361 gttgccgccg cctgcaccat cgtcgaagcc ctcaacctca acctcggcta ctggatactg
     6421 ctgaccgccc ttttcgtctg ccaacccaac tacaccgcca ccaaaagccg cgtgtaccaa
     6481 cgcatcgccg gcaccgtact cggcgtaatc gtcggctcgc tcgtccccta cttcaccccc
     6541 tccgtcgaaa ccaaactctg gatcgtcatc gccggtacca ccctcttttt catgacccgc
     6601 acctacaaat acagtttttc caccttcttc atcaccattc aggcactgac cagcctctcc
     6661 ctcgcaggtt tggacgtata cgccgccatg cccgtgcgca tcatcgacac cattatcggc
     6721 gcatcccttg cctgggcggc ggtcagctac ctgtggccag actggaaata cctcacgctc
     6781 gaacgcaccg ccgcccttgc cgtatgcagc agcggcacat acctccaaaa aattgccgaa
     6841 cgcctcaaaa ccggcgaaac cggcgacgac atagaatacc gcatcacccg ccgccgcgcc
     6901 cacgaacaca ccgccgccct cagcagcacc ctttccgaca tgagcagcga acccgcaaaa
     6961 ttcgccgaca gcctgcaacc cggctttacc ctgctcaaaa ccggctacgc cctgaccggc
     7021 tacatctccg ccctcggcgc ataccgcagc gaaatgcacg aagaatgcag ccccgacttt
     7081 accgcacagt tccaccttgc cgccgaacac accgcccaca tcttccaaca cctgcccgac
     7141 atgggacccg acgactttca gacggcattg gatacactgc gcggcgaact cggcaccctc
     7201 cgcacccgca gcagcggaac acaaagccac atcctcctcc aacagctcca actcatcgcc
     7261 cggcagctcg aaccctacta ccgcgcctac cgacaaattc cgcacaggca gccccaaaac
     7321 gcagcctgaa aaaatttcgg cattttgtaa aggggggcgg attgtcaggc aggttgcaag
     7381 atagtggata aggctttgcg cagggtaaat gcgtagcaac tgaaccgtca ttccctcaaa
     7441 aacagaaaac caaaaacaga aacctaaaa
//
LOCUS       DAXDXW010000089         7277 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXDXW010000089 DAXDXW010000000
VERSION     DAXDXW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.090"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(125..1987)
                     /gene="hscA"
                     /locus_tag="ACFB64_001867"
     CDS             complement(125..1987)
                     /gene="hscA"
                     /locus_tag="ACFB64_001867"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC1210171.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDADGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRFIELHTRQGVKTPVEVSAEILKTLKLRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCYLLEQNRLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATFSDGMAIDTSISRAEFHNLTQHLVMKTFEPVKQALKDAGVGKNEVK
                     GVVMVGGSTRMPHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMGNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEEFAQI
                     QRDIADLQGRLKDGKAEDIRAAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            2158..3030
                     /gene="hpnD"
                     /locus_tag="ACFB64_001868"
     CDS             2158..3030
                     /gene="hpnD"
                     /locus_tag="ACFB64_001868"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC1210172.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKQDAVTVLYVFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDNAFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            3105..4418
                     /gene="hpnE"
                     /locus_tag="ACFB64_001869"
     CDS             3105..4418
                     /gene="hpnE"
                     /locus_tag="ACFB64_001869"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC1210173.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAILATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGIADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLRRILPHLGEPEAVRVITEKRATTATDAPPPDL
                     SWLHRHRIFPAGDYLHPHYPATLEAAVQSGFASAEACLQSLNDAV"
     gene            4500..5219
                     /locus_tag="ACFB64_001870"
     CDS             4500..5219
                     /locus_tag="ACFB64_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC1210174.1"
                     /translation="MPTLTDKTILVTGASQGLGEQVAKAYAAEGATVILVARHQKKLE
                     KAYDAIVEAGHPEPFAIRFDLMSAEEKEFERFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAGSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            5385..6056
                     /locus_tag="ACFB64_001871"
     CDS             5385..6056
                     /locus_tag="ACFB64_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC1210175.1"
                     /translation="MKTVSAAIAFAAAAVSLTGCATESSRSLEVAKVASCNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDSEDRLGSQAKTILVTHLQQTNRFNVLNRTNLSALKQE
                     SGISGKAQNLKGADYIVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSTQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVDNLVQAVDNGA
                     WQSNR"
     gene            6066..6432
                     /locus_tag="ACFB64_001872"
                     /pseudo
     CDS             6066..6432
                     /locus_tag="ACFB64_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
     gene            6429..7076
                     /locus_tag="ACFB64_001873"
     CDS             6429..7076
                     /locus_tag="ACFB64_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC1210176.1"
                     /translation="MKPLILGLAAVLALSACQVRKAPDLDYTSFKESKPASILVVPPL
                     NESPDVNGTWGMLASTAAPISEAGYYVFPAAVVEETFKENGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVGAVVNQIANSLTDRGYQVSKTAAYNLLSPYSRNGILKSPRFVEEQPK"
BASE COUNT         1617 a         2123 c         2015 g         1522 t
ORIGIN      
        1 gcccttgatg acggttttca tcgaatagac aatgcctgac ttttccatat cgatgttttt
       61 caagtgtttt cgatccttca gacggcatca ggttatatct atgccgtctg aaaccgttta
      121 agtatcaaat attatcgaca ctctgacctg tcagcgcacg ttggatgttg cggttcatgc
      181 gcttggcggc gaaattgtcg gtgctgcggc tgagtttggc gacagctgca cggatgtctt
      241 cagctttacc gtctttcaga cggccttgca aatcggcgat gtctcgctgg atttgtgcaa
      301 actcttcggc atccagcaaa tcgctgtcca actcgagggc ggcgttgacg gcatcggtca
      361 ggctttcggc ttcgaccacg gcttcggcgc gggcgcgtgc cgccatatct tcggcggcgt
      421 tgcccatgct gtctttgagc atttgggtga tggtgtcatc gtccaaaccg taggagggtt
      481 tgacttcgat ttgcgcctgt acgccggtgc tttgttcttg ggcggaaacg gacagcaaac
      541 cgtcggcatc gatttggaag gtaacgcgga tacgcgccgc acccgccgcc ataggcggaa
      601 tgccgcgcag ggtaaatttg gcaaggctgc ggcagtcgga aacgagctcg cgttcgcctt
      661 gcacgacgtg tatcgtcatc gcggtctgac cgtctttgaa ggtggtaaag tcctgcgcgc
      721 gcgcggtggg gatggtggaa ttgcgcggga tgattttttc cgccaagccg ccgtaggttt
      781 ccaaaccgag cgacaagggg gtaacgtcca acagcagcca ttcgccgtcg gctttgttgc
      841 ctgcgaggac gtttgcctgt atggctgcgc cgagcgcgac gacttcgtcg ggattgaggt
      901 tgttcagcgg ggtttgtccg aaaaaggtgg cgactgcctg ttggacgtgc ggcatacggg
      961 tcgaaccgcc gaccataacc acgcctttga cttcgttttt accgacaccg gcatctttca
     1021 acgcctgttt gaccggctcg aacgttttca tcaccaaatg ctgcgtcagg ttgtggaact
     1081 cggcgcgact gatgcttgtg tcgattgcca taccgtctga aaacgtcgcc tgaatgcgcg
     1141 cttcggtttg cgtggtcaat tgttctttgg cggcgcggac gagcgagagc aggagttggc
     1201 tgtcttgttc gttgagttgg gagagtctgt tttgttcgag caggtagcag aacaaacgat
     1261 ggtcgaagtc atcgccgccc aacgcgctgt tgccgccggt ggctttgact tcaaacagtc
     1321 ctttggtcag ttgcaacacg gatacgtcga atgtgccgcc gcccaaatcg tacacgacaa
     1381 acgtgccttc cgaggcgttg tccagcccgt aggcgattgc ggcggcggta ggttcgttga
     1441 gcaggcgcaa tacgttcaaa ccggccagac gcgcggcatc tttggtggcc tggcgttgcg
     1501 cgtcgtcgaa ataggcgggg acggtaatca ccacgccgac caaatcgccg cccaaggttt
     1561 cttcggcacg caatttgagg gttttgagga tttccgccga cacttcgaca ggcgttttca
     1621 ccccctgccg cgtatgcagt tcgataaagc gttgattgtc gccgaaacgg taaggcaggt
     1681 aatgcgtatt ttgatgcaga tcggcaagag tccgcccgat aaggcgtttg gcggagctga
     1741 cggtgttcag cgggtcggtt ttttgggcgg acagggcggt tttgccgact tcaatgccgc
     1801 cgttttccag atagcggacg acggaaggca gggtaacgcg cccgtcggca tcgggcaggc
     1861 aggcggcact gccgctgcgg acggtggcga ccaagctgtt ggtcgtaccc aaatcgatgc
     1921 ctgccgccaa acggtgccgg tgcggggcgg cggacatacc cggttctgaa atctgcaaaa
     1981 gagccataaa ttttgcctgt cggtgcggtg tgtaaaggaa ggaaggcggc attttaacag
     2041 aatttgcgcc gatagttgag caaaccgcta aggattgggc cgtgccgccg aaattgccct
     2101 ctaccgccaa ataccgataa aatccgcctt tcccgcccat cattaggaat ataaatcgtg
     2161 aaaggactcg actattgccg ccaaaaagca gaggaaagcc gctccagttt tttgtcgggc
     2221 ttccgtttcc tgactcagga aaaacaggat gcggtaacgg ttctgtatgt cttttgccgc
     2281 gaattggacg atgtggttga cgaatgttcc aaccccgatg ttgcacaggc aacattgaac
     2341 tggtggcgcg gcgatttgga caatgcgttc ggcggcgcga tgccggaaca ccccgtcaat
     2401 caggccttgc ggcaggttaa ggaaaccttc aagctgccga aatatgaact ggaagcctta
     2461 atcgacggga tgcagatgga tttggttcag gcgcgttacg gcagttttga ggaattgaaa
     2521 ctgtattgcc gccgcgtcgc gggcgtggtc ggctgcctga ttgcgcggat tttggggttt
     2581 tcagacggca aaacgctgga atacgcggat aaaatgggtc ttgcgctgca actgaccaac
     2641 atcatccgcg atgtcggcga agatgcgcgc aggggacgga tttacctgcc gatggaggaa
     2701 atgcagcggt ttgacgtacc cgcaagcgtg attttgcaat gcagcccgac gggcaatttt
     2761 gccgaattga tggcgttcca aatcaaacgc gcccgtgaaa cctaccgcga agccgtatcg
     2821 ctgctgcctg atgccgataa aaaagcccaa aaagtcggac tggttatggc ggcggtttat
     2881 tacgcgctgt tgaacgaaat cgaccgagac ggcgcgcaaa acgtcctcaa atacaaaatc
     2941 gccctccctt cgccgcgcaa aaaacgcatt gccctgaaaa cctggttatt cggattcaaa
     3001 ccgcgccccg gcacgccgga acgggcataa ggcgcatact gcccttcagg aaaacccaac
     3061 ccccgccgcc ccgaaaaacg gggtttcaac cattggagga ataaatgatg aacacgccgc
     3121 atccgcgccc gaaaatcgcc gtcatcggcg caggctgggc cggcttgtcc gccgccgtca
     3181 ccttggcgcg gcacgccgac gttaccctgt ttgaagccgg ccggcaggcg ggcggaaggg
     3241 cgcgcacact ggccggaaat accgacggtt tcggtttttt ggacaacggg cagcacattt
     3301 tgctcggcgc ataccggggc gtgttgcgcc tgatgaaaac catcggttca gacccccgtg
     3361 ccgccttttt gcgcgtaccg ctgcactggc atatgcacgg cggtttgcag ttccgcgccc
     3421 tccccctgcc cgcgccgctg catattttgg gcggcgtgct gcttgcccgg cgcgtaccgt
     3481 ccgcattcaa agccaaactg cttgccgata tgtccgattt gcagaagtcc gcacgcctcg
     3541 gacagcccga cacgacagtt gcacaatggc tgaaacagcg gaacgtgccg cgtgccgccg
     3601 taatgcagtt ttggcagccc ttggtctggg gcgcgctcaa cacgcctttg gaaaccgcaa
     3661 gcctgcgcgt gttgtgcaac gttttgtccg acggcgtgct gacgaaaaaa tccggcagcg
     3721 actatctcct acccaaacag gatttgggcg caatcgtcgc cgaacccgcc ttggcggagc
     3781 ttcaacggct cggcgcggac atccgcctcg aaacgcgcgt atgccgtctg aacaccctcc
     3841 cggacggaaa agtcctcgtc aacggcgaag ccttcgatgc cgccatactt gccaccgcgc
     3901 cctaccacgc cgccgcgctc ctgcccgaag gcacgcccga acacgttcag acggcatatc
     3961 aaaaccttcg ctaccacgcc attaccaccg tctatctgcg ctacgccgaa cccgtccgcc
     4021 tgcccgcccc gctgaccggc attgccgacg gcacggcaca atggctgctt tgccggggca
     4081 ggctcggact gcccgaaaac gaagtctccg ccgtcattag cgtttccgac cgcgtcggcg
     4141 cgtttgcaaa ccgggcgtgg gcggacaaag cccacgccga cctcagacgc atccttccgc
     4201 atttgggcga acccgaagcc gtgcgcgtca tcaccgaaaa acgcgccaca accgcaaccg
     4261 atgccccgcc gccggatttg tcgtggctgc accggcaccg tatcttcccc gccggcgact
     4321 acctccaccc gcactacccc gccacgctcg aagccgccgt acaatcaggt ttcgcgtcgg
     4381 cggaagcctg cctgcaaagc ctgaacgatg ccgtctgaaa acgccggccg gcatcgggac
     4441 gctttacaag gtgcggcaaa acgctaaaat acattaaccg tcaaacccaa aggacaacca
     4501 tgccgacact gaccgacaaa accatcttag ttaccggcgc atcgcaaggc ttgggcgaac
     4561 aggtcgccaa agcctacgcc gccgaagggg cgaccgtaat tctggtggca cgccatcaga
     4621 aaaaactgga aaaagcctat gacgcgattg tcgaagccgg acaccccgaa cccttcgcca
     4681 tccgtttcga cctgatgagt gcggaagaaa aagaattcga acggtttgcc gccaccattg
     4741 ccgaagccac gcaaggcaaa ctggacggca tcgtccactg cgccggctat ttttacgccc
     4801 tctcgccgct ggatttccaa accgtcgccg aatgggtcaa ccaataccgc atcaacaccg
     4861 tcgccccgat ggggctgacc cgcgccctgt tcccgctgct gaagcagtcg cccgacgcgt
     4921 ccgtcatctt cgtcggcgaa agccacggcg aaacacccaa agcctactgg ggcggcttcg
     4981 gcgcgtccaa agccgcgttg aactacctgt gcaaagtcgc cgccgacgaa tgggaacgct
     5041 tcggcaacct gcgcgccaac gtcctcgtcc ccggccccat caattccccg caacgcatca
     5101 aatcccatcc gggcgaagcc ggaagcgaac gcaaaagcta cggggacgta ctgcccgcat
     5161 ttgtctggtg ggcaagtgcc gaaagcaaag ggcggagcgg cgaaatcgtt tacctctaaa
     5221 tccggaaaag gcaggcatcc cgcccgcgcc gcgcttccgt tttgaaaaat atccgtacct
     5281 tccgcgcgca tccgtcccgc acagacaaac accttgactt aaatttacat aaggacaata
     5341 atgataaccc gttatttccc taactggacc ttagggtaaa ttttatgaaa accgtttccg
     5401 ccgccatcgc ttttgccgcc gctgccgttt cactgaccgg ctgtgcgacc gagtcctcac
     5461 gcagcctcga ggttgcaaaa gtcgcctcct gcaatacgca atatcacggt gttcgcaccc
     5521 cgatttccgt cggaacattc gacaaccgct ccagcttcca aaaaggcatt ttctccgaca
     5581 gtgaagaccg tctgggcagc caggcaaaaa ccatcctggt aacacacctg caacaaacca
     5641 accgcttcaa cgtactgaac cgcaccaacc ttagcgcatt gaaacaggaa tccggcattt
     5701 ccggcaaagc gcagaacctg aaaggcgcag attatatcgt taccggcgat gtaaccgaat
     5761 tcggacgcag agatgtcggc gatcatcagc tcttcggcat tttgggtcgc ggcaaatcgc
     5821 aaatcgccta tgcaaaagtg gctctgaata tcgtcaacgt caatacttcc gaaatcgtct
     5881 attccacaca gggcgcgggc gaatacgcac tttccaaccg cgaaatcatc ggtttcggcg
     5941 gcacttccgg ctacgatgcg actttgaacg gcaaagtttt agacttggca atccgcgaag
     6001 ccgtcgacaa cttggttcag gctgtcgaca acggcgcatg gcaatccaac cgttaaggag
     6061 aaatgatgaa tccgaaaacc ttgggccgtt tgtcgctgtg tgcggcggtc ttggctctga
     6121 ccgcctgcgg cggcgggcat aaaaacctgt attattacgg cggttatccc gataccgtct
     6181 atgaaggttt gaaaaacgac gacacttcgt tgggcaagca gaccgaaaag atggaaaaat
     6241 actttgcgga agccgccaac aaaaaaaatg aatgccgccc cgggtgcgca cgcccatttg
     6301 ggactgctgc tttcccgttc gggagacaaa gagggcgcgt tccgccaatt tgaagaagag
     6361 aaaaggctgt ttcccgaatc gggcgtattt atggacttcc tgatgaaaac cggtaaagga
     6421 ggcaagcgat gaaacctttg attttagggc ttgccgccgt gttggctctg tctgcctgcc
     6481 aagttcgaaa agctcccgac ctcgactaca cgtcattcaa agaaagcaaa ccggcttcaa
     6541 ttttggtggt tccgccgctg aacgagtcgc ctgatgtcaa cggcacttgg gggatgctgg
     6601 cttcgaccgc cgcgccgatt tccgaagccg gctattacgt cttccccgcc gcagtcgtgg
     6661 aggaaacctt caaagaaaac ggcttgacca atgccgccga tattcacgcc gtccggccgg
     6721 aaaaactgca tcaaattttc ggcaatgatg cggttttgta cattacggtt accgaatacg
     6781 gcacttcata tcaaatttta gacagcgtga cgaccgtatc cgccaaagca cggctggtcg
     6841 attcccgcaa cgggaaagag ttgtggtcgg gttcggccag catccgcgaa ggcagcaaca
     6901 acagcaacag cggcctgttg ggggctttgg tcggcgcagt ggtcaatcag attgccaaca
     6961 gcctgaccga ccgcggttat caggtttcca aaaccgccgc atacaaccta ctgtcgccct
     7021 attcccgcaa cggtatcttg aaaagtccga gattcgtcga agagcagccc aaataagcat
     7081 atgccgtctg aacctctccg agcttcagac ggcatatgcg aatcttgata cttgcaagcc
     7141 gcaccgccca aaatcaggcg accggtttca atatccgaac aaggttcggg ttctgtattt
     7201 tgcagaaccc gaacctttta ttcaaattta aacttaattc cggcgtggtt gtatggtaaa
     7261 ttatagtgga ttaaatt
//
LOCUS       DAXDXW010000090         7172 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXDXW010000090 DAXDXW010000000
VERSION     DAXDXW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.091"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            231..2372
                     /gene="fetA"
                     /locus_tag="ACFB64_001874"
                     /gene_synonym="frpB"
     CDS             231..2372
                     /gene="fetA"
                     /locus_tag="ACFB64_001874"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC1210177.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNNGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFAVGDTKSRINITR
                     QAPAYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSEFEIKDKEKATNEEKKK
                     NRENEKIAKAYRLTNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTHDGKTVSSS
                     SLNPSFGVIWQPREHWSFSASHNYAGRSPRLYDALQTHGKRGIISIADGTKAERAHNT
                     EIGFNYNDGTFAANGSYFRQTIKDALANPQNRHDSVAVREAVNAGYIKNHGYELGASY
                     RTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQTGRTWTASLAYRFKNPNLEIGW
                     RGRYVQKAVGSILAAGQKDRDGKLENVVRQGFGVNDVFANWKPLGKDTLNVNLSVNNV
                     FDKFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
     gene            2524..3489
                     /locus_tag="ACFB64_001875"
     CDS             2524..3489
                     /locus_tag="ACFB64_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC1210178.1"
                     /translation="MLRLTTLTLCTVLALGACSPQNSDSAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVVLVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            3507..3698
                     /locus_tag="ACFB64_001876"
     CDS             3507..3698
                     /locus_tag="ACFB64_001876"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210179.1"
                     /translation="MPSETRMQTASCVSDGIARYGRYRGNGGKRFDWNLPHPLPVRRF
                     QTRLSAPTVRTALFESPTP"
     gene            3717..4685
                     /locus_tag="ACFB64_001877"
     CDS             3717..4685
                     /locus_tag="ACFB64_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC1210180.1"
                     /translation="MTAKPFSLNLANLLLPAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGSSMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     KNEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIVSRLMGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            4675..5649
                     /locus_tag="ACFB64_001878"
     CDS             4675..5649
                     /locus_tag="ACFB64_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC1210181.1"
                     /translation="MPSEKNIGFMAGSSRPLRVAFALLLVSCILFMTVNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYTSLPLTGKFGFELVVMMGGSLLLFYTLIRQGGRDLPHMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVRSELLGIGALVLLVSAAVVWRERYRSDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            5694..6320
                     /locus_tag="ACFB64_001879"
     CDS             5694..6320
                     /locus_tag="ACFB64_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFC1210182.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVGGSASAKELERFGQLWQARVKAFLTESADDPQFVIVREL"
     gene            6404..7162
                     /locus_tag="ACFB64_001880"
     CDS             6404..7162
                     /locus_tag="ACFB64_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC1210183.1"
                     /translation="MITIRNVSYRIGTHPILDNVSLDIPEGGITALIGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTHEHKRTTVAVLHDINQAAAYADHVVAMKNGKAALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
BASE COUNT         1669 a         2161 c         1840 g         1502 t
ORIGIN      
        1 tgtactgcct gcggcttcgc cgccttgtcc tgatttttgt taatccacta tacatttccg
       61 acaaaacctg tcaacaaaaa acaacgcttc gcaaataaaa acgataatca gctttacaca
      121 accccccccc cgctaatata aacaaaaata attattatta ttttttctta tcctgccaaa
      181 ccttaacggt ttggcttaac ttcccttcat acactcaaaa ggacgaacaa atgaacgccc
      241 cgtttttccg cctcagcctg ctctcgctca cacttgccgc cggctttgcc cacgcggcag
      301 aaaataatgc caatgtcgca ttggataccg ttaccgtaaa aggcgaccgc caaggcagca
      361 aaatccgtac caacatcgtt acgcttcaac aaaaagacga aagcaccgca accgatatgc
      421 gcgaactctt aaaagaagag ccctccatcg atttcggcgg cggcaacggc acgtcccaat
      481 tcctgacgct gcgcggtatg ggtcagaact ctgtcgacat caaggtggac aacgcctatt
      541 ccgacagcca aatcctttac caccaaggca gatttattgt cgatcccgct ttggttaaag
      601 tcgtttccgt acagaaaggc gcgggttccg cctctgccgg tatcggcgcg accaacggcg
      661 cgatcatcgc caaaaccgtc gatgcccaag acctgctcaa aggcttggat aaaaactggg
      721 gcgtgcgcct caacagcggc tttgccagca acaacggcgt aagctacggc gcaagcgtat
      781 tcggaaaaga gggcaacttc gacggcttgt tctcttacaa ccgcaacgat gaaaaagatt
      841 acgaagcagg caaaggtttc cgcaatgtca acggcggcaa aaccgtaccg tacagcgcgc
      901 tggacaaacg cagctacctc gccaaaatcg gaacaacctt cggcgacggc gaccaccgca
      961 tcgtgttgag ccatatgaaa gaccaacacc ggggcatccg cactgtgcgt gaagagtttg
     1021 ccgtcggcga cacaaaatca cggataaata ttacacgcca agcccctgcc taccgcgaaa
     1081 ccacccaatc caacaccaat ttggcgtaca ccggcaaaga tttgggcttt gtcgaaaaac
     1141 tggatgccaa cgcctatgtg ttggaaaaga aacgctattc cgccgatgac aaagataacg
     1201 gctacgcagg caatgtaaaa ggccccaacc atacccgaat taccactcgg ggtatgaact
     1261 tcaacttcga cagccgcctt gccgaacaaa ccctgttgaa atacggcatc aactaccgcc
     1321 atcaggaaat caaaccgcaa gcgtttttga actcggaatt tgaaataaaa gataaagaaa
     1381 aagcaactaa tgaagagaaa aagaagaacc gtgaaaatga aaaaattgcc aaagcctacc
     1441 gcctgaccaa cccgaccaaa accgataccg gcgcgtatat cgaagccatt cacgagattg
     1501 acggctttac cctgaccggc gggctgcgtt acgaccgctt caaggtgaaa acccacgacg
     1561 gcaaaaccgt ttcaagcagc agcctcaacc cgagtttcgg cgtgatttgg cagccgcgcg
     1621 aacactggag cttcagcgcg agccacaact acgccggccg cagcccgcgc ctgtatgacg
     1681 ctctgcaaac ccacggcaag cgcggcatca tctcgattgc cgacggcacg aaagccgaac
     1741 gcgcgcacaa taccgaaatc ggcttcaact acaacgacgg cacgtttgcc gcaaacggca
     1801 gctacttccg gcagaccatc aaagacgcgc ttgccaatcc gcaaaaccgc cacgactccg
     1861 tcgccgtccg cgaagccgtc aacgccggct acatcaaaaa ccacggttac gaattgggcg
     1921 cgtcctaccg caccggcggc ctgaccgcca aagtcggcgt aagccacagc aaaccgcgct
     1981 tttacgatac gcacaaagac aagctgttga gcgcgaaccc tgaatttggc gcacaaaccg
     2041 gccgcacttg gacggcctcc cttgcctacc gcttcaaaaa cccgaatctg gaaatcggct
     2101 ggcgcggtcg ttatgttcaa aaagccgtgg gttcgatatt ggcggcaggt caaaaagacc
     2161 gcgacggcaa attggaaaac gttgtacgcc aaggtttcgg tgtgaacgat gtcttcgcca
     2221 actggaaacc gctgggcaaa gacacgctca atgtcaatct ttcggttaac aacgtgttcg
     2281 acaagttcta ctatccgcac agccaacgct ggaccaatac cctgccgggc gtgggacgtg
     2341 atgtacgcct gggcgtgaac tacaagttct aaaacgcaca tcccgaaaaa atgccgtctg
     2401 aaagcctttc agacggcatc tgtcctgata atttgatata gattatcatt tatcctttct
     2461 aaagccgttc cggtttgtcc gaccggcggc tttgccccaa tatccccatt ttggagacac
     2521 cctatgttgc gtttgaccac attgaccctc tgcaccgtcc tcgccttggg cgcgtgttcg
     2581 ccgcaaaatt ccgactctgc cccacaagcc aaagaacagg cggtttccgc cgcacaatcc
     2641 gaaagcgcgt ccgttaccgt caaaaccgcg cgcggcgacg ttcaaatacc gcaaaacccc
     2701 gaacgcatcg ccgtttacga tttgggtatg ctcgacacct tgagcaaact gggcgtgaaa
     2761 accggtttgt ccgtcgataa aaaccgcctg ccgtatttag aggaatattt caaaacgaca
     2821 aaacctgccg gaactttgtt cgagccggat tacgaaacgc tcaatgccta caagccgcag
     2881 ctcatcatca tcggcagccg cgccgccaag gcgtttgaca aattgaacga aatcgcgccg
     2941 accatcgaga tgaccgccga caccgccaac ctcaaagaaa gtgccaaaga gcgcatcgac
     3001 gcactggcgc aaatcttcgg caaacaggcg gaagccgaca agctgaaggc ggaaatcgac
     3061 gcgtcttttg aagccgcgaa aaccgccgcg caaggcaaag gcaaaggttt ggtggttttg
     3121 gtcaacggcg gcaagatgtc cgccttcggc ccgtcttcac gactgggcgg ctggctgcac
     3181 aaagacatcg gcgttcccgc tgtcgatgaa tcaattaaag aaggcagcca cggccagcct
     3241 atcagcttcg aatacttgaa agagaaaaat cccgactggc tgtttgtcct cgaccgcagc
     3301 gcggccatcg gcgaagaggg tcaggcggcg aaagacgtgt tgaacaatcc gctggttgcc
     3361 gaaacgaccg catggaaaaa aggacaggtc gtttacctcg ttcccgaaac ttatttggca
     3421 gccggcggcg cgcaagagct gctgaacgcg agcaaacagg ttgcggatgc gttcaacgcc
     3481 gcgaaataat gaaacggcgg cattcgatgc cgtctgaaac acggatgcaa accgcttcct
     3541 gtgtttcaga cggcattgcc cgatacgggc gttatcgcgg gaatggcggc aagcgttttg
     3601 attggaactt gccccatcct ctgcccgtcc ggaggtttca aacaaggctt tccgctccga
     3661 cggttcggac tgccttgttt gaatctccta cgccgtaacg cttttccctt ctgtttatga
     3721 ctgccaaacc tttttccctc aacctggcca acctcctgct gccggcggtg ttgtttgccg
     3781 tcagcctgtc ggtcggcgtt gccgatttcc gctggtcgga tgtgttttcg ctgtccgaca
     3841 gccaacaggt catgttcatc agccgcctgc cgcgcacgtt tgcgattgtg ctgacgggct
     3901 cgtcgatggc ggtggcgggg atgattatgc agattctgat gcgcaaccgt tttgtcgagc
     3961 cttctatggc gggtgcgggc caaagtgcgg ctttgggttt gcttctgatg tccctgctgc
     4021 tgcctgccgc gccgctgccg gtcaaaatgt cggttgccgc cgttgccgcg ctgatcggga
     4081 tgttggtctt tatgctgctg atccgccgcc tgccgccgac ggcgcaactg atggtgccgc
     4141 tggtggggat tattttcggc ggcgtggttg aggcggtggc gacgtttatc gcgtataaaa
     4201 acgaaatgct gcaaatgctc ggcgtgtggc agcagggcga tttttcgagc gtgctgctgg
     4261 ggcggtacga gctgctttgg attacgggcg gtttggcggt gtttgcctac ctgattgccg
     4321 accggctgac gattttgggg ctgggcgaga cggtcagcgt gaatttgggt ttgaaccgga
     4381 cggcggtgtt gtggtcgggt ttgattattg tggcactgat tacatcgctg gtcattgtaa
     4441 cggtcggcaa tattccgttt atcgggctgg tcgtgccgaa tatcgtcagc cgcctgatgg
     4501 gcgacaggct gcgccaaagc ctgcctgcgg tcgccctctt gggcgcgtct ttggttttat
     4561 tgtgcgacat tatcggacgc gtgattgtgt ttccgtttga aattccggtc tctacggttt
     4621 ttggtgtgtt gggtacggct ttgtttttgt ggcttttatt aaggaaacct gcccatgccg
     4681 tctgaaaaaa atatcggttt tatggcagga agcagccgtc cgttgcgggt cgcctttgcg
     4741 ctgttgctgg tttcctgcat cctgtttatg acggtcaacg tcaaaggcga ttgggacttt
     4801 gtcttgcacc tgcgcctgac caagcttgcc gcgctgctga tggtcgccta tgcggtcggc
     4861 gtgtccactc aactcttcca aacgctgacc aacaacccga ttctgacccc ttcgattttg
     4921 ggtttcgatt cgctgtatgt gtttttgcag accttgctgg tgtttacgtt cggcggcgtg
     4981 ggctatacat ccctgccgtt gacgggcaaa ttcggctttg aactggttgt tatgatgggc
     5041 ggctcgctgc tgctgtttta cacgctcatc cgtcagggcg ggcgcgattt gccgcacatg
     5101 attttaatcg gcgtgatttt cgggattttg ttccgcagcc tttcctcgct gctttcgcgc
     5161 atgatagacc ccgaagaatt taccgccgcg caggcgaata tgtttgccgg attcaatacc
     5221 gtccgcagcg agcttttagg cataggcgcg ctggtcctgc tcgtcagcgc ggcggtcgtt
     5281 tggcgcgaac gctaccgctc ggacgtacac cttttggggc gcgaccaagc cgtcaatttg
     5341 ggcatcagct acacgcgcaa caccttatgg atactgcttt ggattgccgc attggtggcg
     5401 acggcgaccg ccgtggtcgg cccggtaagc tttttcgggc ttctcgccgc ctcgcttgcc
     5461 aaccactttt ccccgtccgt gcgccattcc gtccgcctgc cgatgacggt ttgcgtcggc
     5521 ggcatcctct tggtcggcgg acaaaccgta ttcgaacact tcttgggcat gaaggcggta
     5581 ttaagcgtgg tggtcgaatt tgcgggcgga ctcgttttcc tctatctcgt tttaaaacac
     5641 aaaaaatgac ggatgccgtc tgaacggcag cccgccccga aaggacaaac catatgacgc
     5701 aagaacgttt acccgaattt ttcgaccgcg ccccgacgct gaccgtacaa gacccgcttg
     5761 ccgcattcct cggcgcggcc gaaaacggca tcctcactta ccgctacgcc gatgccgtgc
     5821 gcctgtgcgg acattcctgc ccgaccgtcg cgggcgcgta cctgatggtt atcaaaggcc
     5881 tgaaagcact ttacggcgaa gagctgcccg aacgcggcgg catcgaagcc gccatgcagg
     5941 gaacgcgcga cgaaggcacg gtcggcgtaa ccgcgtccgt cgtccaactc cttaccggcg
     6001 cagcccccga aaccggcttc ggaggcatcg gaatgcaggg acgcttcgcc cgccgcaacc
     6061 tcttatcctt tggtgcaggc gaaatcaacg gcacactgac cctgcgccgc aaagacaacg
     6121 gcaaaaccgt tgccgtcagc ctcaacgccg ccctgcaacc ctttgcacct caaatgcgcg
     6181 acatcatgcc caaagccgtc ggcggcagcg caagcgcaaa agaactcgaa cgcttcggac
     6241 aactttggca ggcacgcgtt aaagcatttt tgaccgaatc ggcggacgac ccgcagttcg
     6301 tcatcgtccg cgaactgtga acgttcagac ggcattccga attccaaatg ccgtccgaac
     6361 ccccgccaaa caacaacaaa cctacgcccg acaagcatcc gccatgatta ccatccgcaa
     6421 cgtcagctac cgcatcggca cacaccccat cctcgacaac gtcagcctcg acatccccga
     6481 aggcggcatt accgcgctaa tcggccccaa cggtgcgggc aaatccaccc tgttttcctt
     6541 tatggcgcgg ctgcgcccgc ttgaaagcgg cagcatcgcc taccgaggca aaaaccttgc
     6601 cggcaccccc accgccgaac tcgcccgcac gctctccatc ctcacccaag aaaacagcat
     6661 catgagccgc atcaccgtgc gcgacctgct gatgttcggc cgttacccct accatcaagg
     6721 cagaccgact gccgaatgcc gccgtatcgt taacggtgca atcgaagaat tccacctgca
     6781 ggacctctcc gaccgctacc tgaccgaact ctccggcgga caacgccaac gcgccatgat
     6841 tgcgatggtg ttctgccaaa gcaccgacta cgtccttttg gacgaaccgc tgaacaacct
     6901 cgatatgtat cacgcccgct cgctcatgca gattctgcaa aagctcacgc acgaacacaa
     6961 acgcaccacc gtcgccgtat tgcacgacat caaccaggcc gccgcctacg ccgaccacgt
     7021 cgtcgccatg aaaaacggca aagccgccct gaccggcaca cccgaagaag tgttcaccgt
     7081 acacaacatc aaagaactgt tcgacatgga cgtggacgta ctcgactacg aaggcaaaaa
     7141 actcatcgtc caccacatct gaagctcaaa aa
//
LOCUS       DAXDXW010000091         7079 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXDXW010000091 DAXDXW010000000
VERSION     DAXDXW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.092"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..1967)
                     /locus_tag="ACFB64_001881"
     CDS             complement(<1..1967)
                     /locus_tag="ACFB64_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFC1210184.1"
                     /translation="MSKKTPLSQGFIARVAAGVRYAFTGNADGWFDAGEPPAPAAQQA
                     EGRRFDYEPFYNVGHSKPREREAVGFAQLRALADNYDVLRLVIEARKDQMECLKWTIQ
                     KRDVESTEDDESQRKDRKVDEAVAFFRSPDKEHTWADWLRILLEDLFVIDAPCIYPRK
                     TLGGGLYALEVMDGATIKRVLDNTGRMPLPPDTAYQQILHGMAAVDYTADELIYRSRN
                     NRSYKVYGYSPVEQIIMTVNIALKRQVHALEYYTAGSVPDALVGVPETWSADDIRRFQ
                     EYWDLLLSGETAQRRKMRFVPGELSRNFRETKQPPLKDVYDEWLARVVCFAFSVEPTP
                     FVAQVNRSVAETSREQSLSDGMGSLKNWVKALIDDVLARYMDMAAYEFVWKGEESLNP
                     KEQAEIYAIYKNAGILTADEIRAELGKEPLPGQGQPEPDKQDGRKPEEPPNQGAEKLG
                     KSESPMSEDESAALIEAYLLTRIDGLAEQIAALIEGAAVDWQAGDLAAELSRAAGVVA
                     NGLDFGDWSGLSDVVEPIIRRVAEDGAVAALLRVMPEPAAGMVTNIRSRAVKWAHERA
                     AEMVGMKRAGGGLVRNPAAEWQITEGTREMIRAQVAEAMRNGDSVQELAARLKESHAF
                     GNARARTIARTETAMADGMGNLIGWEGTGLVA"
     gene            complement(1964..3385)
                     /gene="terL"
                     /locus_tag="ACFB64_001882"
     CDS             complement(1964..3385)
                     /gene="terL"
                     /locus_tag="ACFB64_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFC1210185.1"
                     /translation="MALGQFDDVETSVIRSLSSASLYMFTRRMFYQRRGYVWQRANHH
                     APICNALERVFNGETKRLIINIPPRYSKTEIAVVNFIAWAMGRVPDCEFIHASYSAAL
                     AVNNSVQIRNLVQHEEYRAIFPDLALAGESGHHWKTTAGGVMYATGAGGTITGFGAGR
                     HREGFGGCIIIDDPHKADEARSEVRRQNVIDWFQNTVESRKNSPDTPIILIMQRLHEK
                     DLAGWLLDGGNGEEWEHLCLPAIQEDGTALWPEKHDIETLRRMEQAAPYVFAGQYLQK
                     PAPPDGGTFKPDNLQFVKALPAGNIRWVRAWDLASTANGGDYTAGGRLGVTEDGRYII
                     ANVVRGRYGADERDRILRNTAQKDGVKTKISIPQDPGQAGKSQTLYLTRQLAGFSVSA
                     GPESGDKVTRAGPFAAQVNIGNVMVLDDGTWDTDALIAEMRMFPNGRHDDQIDCLGRA
                     FGELLDTRTGMIDFLRSQVEAVK"
     gene            complement(3447..3896)
                     /locus_tag="ACFB64_001883"
     CDS             complement(3447..3896)
                     /locus_tag="ACFB64_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210186.1"
                     /translation="MSGTKRKLGRPTDYTKDMADKICEKIANGRSLRSICAEDGVPPM
                     KTIYRWLEANEEFRHQYARAREKQADYFAEEIIEIADSAQAESAAVSKAKLQIDARKW
                     AASKIAPKKYGDKTELDVKSGDGSMRAAVRLDAEEYRKIAEDVLRRV"
     gene            complement(4189..5016)
                     /locus_tag="ACFB64_001884"
     CDS             complement(4189..5016)
                     /locus_tag="ACFB64_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFC1210187.1"
                     /translation="MNNSVQISNISIHQTEIGLFSLNDLHRASGGEDRHAPRRWLQNI
                     QTNDLIKELEKDGKPSIQSKQRLGTFVCRELVIAYGMWISPKFALQVIQTFLAVSDGI
                     KSAKTTADDRTGLRRAVAALVGRKGIDYSSAYSMIHQRFNVESVEGIPAGKLPEAVAY
                     VHALTLHTGLTGEVLDREPLPAPQPALPISGNALYDLAVAVSYGARAIQMGRDVSLPL
                     KQLGCRQAVTMWTVWAETRSRLKAAANALEALSAHADAEHAEKIRPILPEIRNLSAV"
     gene            complement(5307..6467)
                     /locus_tag="ACFB64_001885"
     CDS             complement(5307..6467)
                     /locus_tag="ACFB64_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFC1210188.1"
                     /translation="MNAAAVSAVFKERIASHAEHVKKVAHICTTEETTKQALILPLLD
                     ILGFSAFDPNKVRAEYQADFPGAKSGERVDYALFCNGAPVMFIEAKSYTENLSNHCPQ
                     LSRYFNATPEIAICAITNGREWRFFTDLSNKNIMDSEPFLTVDVTMLNENDMAQLYQF
                     RHDKFQPDALRSLAEESIYLTAFTESITESLKEVDLDFVRYVAGRANIQRQFTQRYLE
                     SIRHIVKQAVQNTVSSMVVSGLSAPKVQEEAAPVEKEQEDPTAPIIDPENNKIVTTYS
                     ERRLFDLVKSILPDDASIEAKDTESYFGVLVDGKSNRWILRYFDNKQRPSVIFPIELE
                     ESDISNIERCGLEVSGNQVIIDTPENLLRVAWLVIDSYRFCCDDENFKRKPK"
     gene            complement(6558..6965)
                     /locus_tag="ACFB64_001886"
     CDS             complement(6558..6965)
                     /locus_tag="ACFB64_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210189.1"
                     /translation="MNQQEFEFMNDLARAFERRYRDTRSLNRCLSIEGRYMGEEACPH
                     KPEIGLRYGEDAMFLTLQAWAKVDAPQQEAVRISFGIGAKSQAAYEERLQAEIRRRGE
                     GPLHLQTDLGLAAWYRAIRQAAGNDFDLLFEKV"
BASE COUNT         1563 a         2081 c         1691 g         1744 t
ORIGIN      
        1 gcaaccagcc ccgttccttc ccagcctatc aggttgccca taccgtccgc catcgccgtt
       61 tccgttcggg caatggttcg ggcgcgtgcg ttgccgaaag catgggattc tttcaggcgg
      121 gctgccaatt cctgcacact gtcgccgttt cgcatggctt cggctacttg ggcgcgtatc
      181 atttcgcgcg tcccttcggt gatttgccac tcggcggcag gatttcggac aagcccgccg
      241 cccgcccgct tcatgccgac catttcggcg gcgcgttcat gcgcccactt gacggcgcgg
      301 ctgcgaatgt tcgtaaccat accggcggca ggttcaggca ttacgcgcaa caaggcggca
      361 accgccccgt cttccgcaac acgcctgatt atcggctcga ccacatcgga caatcccgac
      421 caatcgccaa aatccaagcc gtttgcaaca acccctgccg cgcggctcaa ttccgccgcc
      481 aaatccccgg cctgccaatc gacggccgca ccctcaatca gcgccgcgat ttgttcggcc
      541 aagccgtcaa tgcgtgtcag caaataagcc tcaataagcg cggcagattc gtcttcgctc
      601 atcgggcttt ccgactttcc caacttttca gccccttggt tcggcggctc ttcgggcttt
      661 cggccgtctt gcttatccgg ttcaggctgc ccctgccccg gtaacggctc cttgcccagt
      721 tcggcgcgga tttcgtcggc ggtcaagatg cctgcgtttt tgtagatggc gtagatttca
      781 gcctgttctt tcgggttgag cgattcctcc cccttccaga caaactcata cgccgccata
      841 tccatgtaac gggcaagcac gtcgtcaatc agggctttta cccagttttt caggctgccc
      901 atgccgtctg aaagcgactg ctcgcggctc gtctctgcca cgctgcggtt tacctgcgcc
      961 acgaacggcg taggctcgac actaaacgca aagcagacga cacgcgccag ccattcgtcg
     1021 taaacgtcct tcaacggcgg ctgcttcgtc tcgcggaagt ttcgggacaa ctcgcccggc
     1081 acgaaacgca ttttgcgccg ctgcgccgtt tcgcccgaca gcagcaaatc ccagtattct
     1141 tgaaaccgcc tgatgtcgtc cgccgaccac gtttcaggca cgccgactaa agcatcgggc
     1201 acgctgcccg ccgtgtagta ttccagcgcg tgaacctgcc gttttaaggc aatattcacg
     1261 gtcatgatga tttgctcgac gggcgaatag ccgtaaacct tgtaacttcg gttattccgc
     1321 gaacggtaaa tcaactcgtc agccgtgtaa tcgaccgccg ccatgccgtg caggatttgc
     1381 tgatacgccg tatcgggcgg taacggcata cgccccgtat tgtccaaaac gcgcttaatc
     1441 gtcgccccgt ccatcacttc gagggcgtac aagccgccgc ccagtgtttt gcgcgggtag
     1501 atacacggcg cgtcaatgac aaacaggtct tccagcaaga tgcgcagcca gtccgcccat
     1561 gtatgttctt tatcgggcga ccggaagaac gcaacggctt catcgacctt tcggtctttc
     1621 cgttgcgatt cgtcgtcttc ggttgattcg acgtcgcgct tctgaatcgt ccacttaagg
     1681 cactccattt ggtctttacg cgcctcgata accaaacgca atacatcgta gttgtcggca
     1741 agggcgcgta attgtgcaaa gcctaccgct tcacgttcgc gcggcttgga atgcccgacg
     1801 ttgtagaacg gctcgtaatc aaaccgccgc ccctctgcct gctgcgcggc aggggccgga
     1861 ggctcgcccg cgtcgaacca cccgtccgcg ttgccggtaa aggcgtaacg gacgccggcg
     1921 gcaacgcggg caataaagcc ttgcgataaa ggtgtctttt tactcatttc acagcctcga
     1981 cctgcgatcg caggaaatca atcatgcccg tccgggtatc cagcagctcg ccaaacgcac
     2041 ggcccaaaca gtctatttgg tcgtcatgcc ggccgttcgg gaacatgcgc atttccgcaa
     2101 tcagcgcgtc cgtgtcccat gtgccgtcgt ccagcaccat cacattgccg atgttgacct
     2161 gtgccgcgaa cggtccggcg cgtgtaacct tgtcgcccga ttcggggccg gcggatacgg
     2221 aaaaacccgc caactggcgg gttaggtata gtgtttggga tttgcctgcc tgaccggggt
     2281 cttgcgggat ggatattttc gttttcacgc cgtctttttg cgccgtgttg cgtaatatcc
     2341 tgtcccgctc gtccgcgccg taccggccgc gcacgacgtt ggcgatgata taccgcccgt
     2401 cttccgtaac gccaagcctg ccgcctgccg tatagtcgcc gccgtttgcg gttgaagcca
     2461 agtcccacgc gcgtacccat ctgatattcc cggcaggcaa cgccttgaca aattgcaggt
     2521 tgtcaggctt gaacgtaccg ccgtcaggcg gcgcaggttt ttgcaaatac tgcccggcaa
     2581 acacatacgg cgcggcctgc tccattcggc gcagtgtttc aatatcatgc ttttcaggcc
     2641 acaacgccgt gccgtcttcc tgaatggcag gcaggcacaa atgctcccac tcttcgccgt
     2701 tgccgccgtc aagcagccag cccgccaagt ctttctcgtg caggcgttgc ataatcagga
     2761 taatcggcgt gtcagggctg ttcttccggg attcgaccgt gttttgaaac cagtcgatga
     2821 cgttctgccg cctgacctcg cttcgcgctt catctgcttt gtgcgggtcg tcaatgatga
     2881 tgcagccgcc gaatccctcc cgatgcctgc ccgcaccgaa acctgtaatc gtaccgcccg
     2941 cacctgttgc gtacatcacg ccgcctgcgg ttgttttcca gtgatggccg ctttcgcctg
     3001 ccagtgccaa atcaggaaaa atcgcccgat actcttcgtg ttgcaccaag ttccgaatct
     3061 gtacggagtt attgaccgcc agcgccgccg aatagctcgc gtggataaac tcgcaatcag
     3121 gcacgcgccc catcgcccac gcgataaagt tcacgaccgc gatttccgtt ttcgagtatc
     3181 gcggcggaat attgataatc aggcgtttcg tttcgccgtt gaaaacacgc tcgagcgcgt
     3241 tgcagattgg cgcatggtga ttcgcccgct gccaaacata gccgcgcctt tgataaaaca
     3301 tccggcgcgt gaacatatac aggcttgcag aacttaaact gcgaattact gatgtttcaa
     3361 catcgtcgaa ttgccctagt gccatttcat tctaacttcc tttggagaaa cggccataca
     3421 gcccctctta tgattagcgt tttatgctaa accctacgca acacatcttc cgctatcttg
     3481 cgatattcct cagcatcaag ccgtacagcc gccctcatgc tcccatcgcc cgatttaacg
     3541 tcaagttccg tcttatcgcc gtatttcttc ggcgcaatct tggaagccgc ccactttcgg
     3601 gcatctatct gcaatttcgc ctttgaaacc gccgcgctct ctgcttgtgc actatcggca
     3661 atctcgatga tttcttcagc gaaatagtcc gcctgctttt ctctcgcgcg cgcgtattgg
     3721 tggcgaaatt cttcattagc ttccaaccaa cggtaaatag ttttcattgg cggcacacca
     3781 tcttcggcac atattgaacg taggcttctg ccatttgcga ttttttcgca tatcttatcg
     3841 gccatgtctt tcgtgtaatc tgtcggacgg cctaatttgc gtttcgtgcc gctcataaac
     3901 cctcctcaaa aaagaaaccg tctaactctg acccctctca gaattagacg gcaaacacac
     3961 actcgccaca taggaaaaac ggaacgccgc tacctgtaca ggcagaagct caaattcgga
     4021 cagcctaaaa cgcaaaaacc cgcacattgt tatgtacggg cttaaaaatt catatccttc
     4081 gggcgtgcga aaatccccgc aagggtaacg atttgaatta tacacctatt gccggaaaaa
     4141 acaacaggcc gtctgaagat tcagacggca tttgtattcc ctactgcatc aaaccgccga
     4201 caggttgcgg atttcgggca gtatcgggcg gattttttct gcgtgttccg cgtcggcgtg
     4261 tgcgcttaag gcttcgaggg cgtttgcggc ggctttgagg cggctgcgcg tttccgccca
     4321 gaccgtccac atcgtaaccg cctgcctgca gccgagctgc ttcagcggca gggaaacgtc
     4381 tctgcccatt tggatggccc gcgcgccgta gctgacggcg acggcgaggt cgtacagggc
     4441 gttgccgctt atgggcaggg cgggttgcgg cgcgggtaac ggttcgcggt caaggacttc
     4501 gcccgtcaag cccgtgtgca gtgtcaatgc gtggacgtag gcgacggctt cgggcagctt
     4561 cccggcaggg atgccttcga cggattcgac gttgaagcgt tggtgtatca tactgtacgc
     4621 tgaggagtag tctatgcctt tgcgtccgac aagtgcggca acggcccggc gcaatccggt
     4681 acggtcgtcg gcggtggttt tggcggactt gatgccgtct gaaaccgcca agaacgtttg
     4741 aatcacttgc aaggcgaatt tcggtgaaat ccacatcccg taggcaataa ccagttcgcg
     4801 acaaacaaaa gtgccaagtc tttgttttga ttgaatagac ggttttccgt ctttttcaag
     4861 ttctttaatt aaatcattgg tttgtatatt ttgaagccag cggcgcggtg cgtgtctgtc
     4921 ttctccgcct gaagcgcggt gtaaatcatt cagactgaat aagccgattt cggtttgatg
     4981 gatagaaata ttagagattt gaactgaatt gttcataatg aagattcctg tagaagttta
     5041 cgaaattagc ccaaacgggc gaccgcgagg ttcgtaagcc ttctacaaac aggctgtgct
     5101 tattccctgt tgaacaggta ttgtattcgc agccctcgcg gtcaaaagta aacttcggtt
     5161 gtgctatcga aacaaacaac caaacaagga aactttagac atgaaaaaat cacattgacg
     5221 gagtgattgc cgctagtgtg tggttcttac gccacgaaca ggaatataaa acaaaccccc
     5281 ctgcacatgc aaggggtttt gttaccttat ttgggtttgc gtttaaaatt ctcgtcatca
     5341 caacagaatc ggtaagaatc aataaccaac caagcaacgc gaagcagatt ttcaggcgta
     5401 tcaataataa cttgattgcc tgacacctca agtccgcatc gttcgatatt cgaaatatcc
     5461 gactcttcca attcgatagg gaagattaca gacggccttt gtttgttgtc aaaataccgt
     5521 aatatccacc tgttactttt accatcaacc aatacgccga aatagctttc cgtatccttt
     5581 gcctcaatac tcgcatcatc aggaagaatt gatttaacca aatcaaacaa ccttctttct
     5641 gaataagtgg taacgatttt attgttttcg gggtcgataa tcggcgcggt cgggtcttcc
     5701 tgctccttct caacaggggc ggcctcctcc tgcactttcg gcgcactcag tcccgataca
     5761 accattgaac taaccgtatt ttgaacagcc tgtttgacga tatgacggat gctttccaaa
     5821 tatcgttggg taaactgccg ttgaatattc gcccttcccg ccacataacg gacgaaatcc
     5881 aaatccacct ctttcaagct ttctgtaatt gattctgtaa atgccgtcag gtatatgctt
     5941 tcttccgcaa ggcttcgcaa tgcatcaggt tgaaacttgt cgtgcctgaa ctgatacagt
     6001 tgcgccatat cattttcatt caacatcgta acatcaacgg tcaaaaacgg ctccgaatcc
     6061 ataatattct tgttggacaa atcggtaaaa aatcgccatt cccggccgtt ggtaatcgcg
     6121 caaatggcta tttcaggcgt ggcattgaag taacgcgaca actgcggaca atgattggac
     6181 aaattttcag tatatgattt tgcttcgata aacataaccg gtgcgccgtt gcaaaacaat
     6241 gcgtaatcga cccgctcccc tgattttgca cctgggaaat ccgcctggta ttccgcccga
     6301 accttattcg gatcgaatgc cgaaaaacct agaatatcca ataaaggcaa aatcaaagcc
     6361 tgttttgtcg tctcctcggt agtacagata tgtgcaacct tcttgacatg ttccgcatgg
     6421 gaagcaatcc gttctttaaa taccgcactt acagccgccg cattcatgtt aatctccttg
     6481 gttaaatttg attgggcaaa ttcccacccc attaagaagt gttaattttc gcaatcatag
     6541 cgcaaccgaa aagaaaatca aaccttctca aacagcaaat caaaatcatt ccctgccgcc
     6601 tgccgtatcg ccctatacca cgcggccaag cccaaatccg tctgcaaatg caggggcccc
     6661 tcgccgcgcc gcctgatttc agcctgcaaa cgttcctcgt aggctgcctg cgacttcgca
     6721 ccgatgccga acgaaatacg gacggcctct tgttgcggcg catccacctt cgcccatgcc
     6781 tgcaaagtaa gaaacatggc atcttcgccg tatctcaagc cgatttcagg cttatgcggg
     6841 caagcttcct cccccatata acggccttcg atactcaaac atctatttag gctgcgcgta
     6901 tcacggtaac gacgctcaaa agcacgcgcc aagtcgttca taaattcaaa ttcttgctga
     6961 ttcatagctt gattaaccct ttttcatgca acaaaaccaa agtccgcatt acaccttccg
     7021 cgaaggccgt ctgaagttcc ccttccgcac aatccgccaa gcggttctat acatcaaaa
//
LOCUS       DAXDXW010000092         7017 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXDXW010000092 DAXDXW010000000
VERSION     DAXDXW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.093"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(19..1803)
                     /locus_tag="ACFB64_001887"
     CDS             complement(19..1803)
                     /locus_tag="ACFB64_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFC1210190.1"
                     /translation="MIERLKAVENQAEAMGRGARSAYLKQQAQELGISLATLYRKLEA
                     VSVKPTRKRRSDAGKTELKPEEAKLISAVLAEAMRRNGKRLMPVRQAVEMLRANGKIE
                     AARIDGETGEVIPLSENTITRALREYKLHSDQLLQPDPVSRMKSEHPNHCWQIDPSLC
                     VLYFLPRQGKDTGLRVMKEEEFYKNKPKNVVKIENDRVWRYTGTDHASGTISVRYYFG
                     GETSANLCDFFIYMMQAKKDIGKDPFRTVPRMVMLDPGSANTSAAFKNLCKPLDVHVQ
                     INKPGNPRAKGQVEKANDIVETAFESGLRFTEVHDIDQLNALSERWMRYYNGTQKHSR
                     HGMTRYQAWNKIKPEQLILPPPAEYCRELAVSAPKEAKVSADLEIRFGGRVYSVKGIK
                     GILVGKKVLVGKKVLVGKNPWEANGARVATYDAEGNEVWVSVPEVVFDEMGFKADAAV
                     IGAEYKAPADTDAQRHRKELDKLAMGAETLEAAAAKRKGKAVPFGGKIDPYKHQEDTL
                     AASNTLFMPKQGQQMDYNKMEVAEQVLSKVEIAKRLKPRVEADGGDWKQAVSVILKHY
                     PEGVTEGRLEEAFERTRTRCRLKLLKTG"
     gene            complement(1796..2669)
                     /locus_tag="ACFB64_001888"
                     /pseudo
     CDS             complement(1796..2669)
                     /locus_tag="ACFB64_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009426280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2706..2990)
                     /locus_tag="ACFB64_001889"
     CDS             complement(2706..2990)
                     /locus_tag="ACFB64_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFC1210191.1"
                     /translation="MATSKKAQRLLRVFLAMDAHPVIGISNKELSDGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRKQQERLNARLQELENRIY"
     gene            complement(2977..3207)
                     /locus_tag="ACFB64_001890"
     CDS             complement(2977..3207)
                     /locus_tag="ACFB64_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC1210192.1"
                     /translation="MEKPLNFKPIPYPQTRGSTAVWFKRNGVCKTHRAKHFNLERTAV
                     EHPLRGKLKGNFGKSHEAAVKFGLKEDADGDE"
     gene            3479..3859
                     /locus_tag="ACFB64_001891"
     CDS             3479..3859
                     /locus_tag="ACFB64_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1210193.1"
                     /translation="MEKTSLFGKRLKEERIKLGLNQAEAAEKCGFSREMWGKWERGEN
                     RPSSEKLFSFSKIGIDIDYVMHGRRGETAAMPSESLSAEEKELLALFRQLGSGSRKEL
                     ADYAAFKLVVEKKAQTALGKVSNG"
     gene            4093..5133
                     /locus_tag="ACFB64_001892"
     CDS             4093..5133
                     /locus_tag="ACFB64_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFC1210194.1"
                     /translation="MKKIITHRIYPKNENRERVEPKISTHLIDLPITARRTLETRLTK
                     ALGNKSHGIEMSVVNTAENSFFQIAAAIQLKEEAEFIEDSAQFAHMLTDAQLNTNAPG
                     GILLVLKGRVGDTGKPFLCVIKAEPQDGFRTKEEDDFITIEFLEELLLTDSARLFKIG
                     FLVAETVRPLEQIQSGNYRAFLYDHLMTQTETRPAASYFYQVFLGMSIAASSRKLTQD
                     FFEWTRNFIDNSDLSDDAKLDAHEALRVTLKSAEATISVNNFAQNHLPQEKRTTYTEF
                     MVEKDFPQNAVSKDIEYIKTRLRKRRSYGFSNGVVILTPPEHTQDYMEIAPTEDGEYT
                     VVLIKGQLQQQK"
     gene            5130..5504
                     /locus_tag="ACFB64_001893"
     CDS             5130..5504
                     /locus_tag="ACFB64_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210195.1"
                     /translation="MSDIDDFKSYLESHQAAFSAWGRFVAEEIQNQLSNVISPVPVAN
                     FLKIEAKPGVKEISSALAKIGRKNYTSPQTQMTDLVGVRFVALLAEHIQIVCEIIESS
                     SQWNAKVSKDFADEIQQNPKAI"
     gene            5501..6139
                     /locus_tag="ACFB64_001894"
     CDS             5501..6139
                     /locus_tag="ACFB64_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210196.1"
                     /translation="MTIQSKHYEIRPKQAFITPENVSIPADLCCEVQVRSLLQHAYAE
                     LVHDNIYKPDGNVPKQAEREVAKSMALMETTDDLFSRTLAILKEANQPQEELLPQLSQ
                     LYQKEIGLVPEVDKKTNMIFLETFQSSISQSSILSDIRSLLNEKKYIAKRIKENAEEM
                     YFFSQPAALLVYWLIEKVGADEVWKKWPLPAYNKNLKFICTDLDKQPSHELF"
     gene            6259..6546
                     /locus_tag="ACFB64_001895"
     CDS             6259..6546
                     /locus_tag="ACFB64_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210197.1"
                     /translation="MIEFVRAKKRLLWAFVLLLAWACGYRYAADKAEAEQTALIAAYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQSTDLAAALSELDKTRNTLQE"
     gene            6620..6841
                     /locus_tag="ACFB64_001896"
     CDS             6620..6841
                     /locus_tag="ACFB64_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210198.1"
                     /translation="MPSDTPIKTVAVAEIPPVPSGLLVEYERPERPAGGSPEQLLNHA
                     VRYGGYYRKLEIQIEGWQNWHTKGRLKHD"
     gene            6834..>7017
                     /locus_tag="ACFB64_001897"
     CDS             6834..>7017
                     /locus_tag="ACFB64_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFC1210199.1"
                     /translation="MTDFADRASEREAEFLVEALAKHQPPSENTAGFSHCEDCGDPIP
                     EAKRKAVRGCTRCVVCH"
BASE COUNT         1728 a         1781 c         1635 g         1873 t
ORIGIN      
        1 gctgttttcg gttgaaacct aaccggtttt caaaagtttc agacggcatc ttgtccgggt
       61 gcgttcaaac gcctcttcca gcctgccctc ggtcacacct tccgggtagt gtttgaggat
      121 gaccgacact gcctgtttcc agtcgccgcc gtctgcctcg acgcggggtt ttaagcgttt
      181 ggcgatttcg accttgctca atacctgctc ggcgacctcc atcttgttgt aatccatctg
      241 ttgcccctgt ttgggcataa agagcgtatt gcttgcggca agcgtatctt cttgatgttt
      301 gtacgggtcg attttgccgc cgaacgggac tgccttgcct ttgcgtttgg cggctgccgc
      361 ctccagcgtt tccgcaccca tcgccagctt gtccagctct ttgcgatgcc gttgcgcgtc
      421 tgtatcggcc ggggctttgt attccgcccc gatgaccgcg gcatcagcct tgaagcccat
      481 ctcgtcaaaa actacttcgg gtacggatac ccaaacctcg ttaccctccg cgtcataagt
      541 ggcgacccgc gccccgtttg cctcccaagg attcttaccg accaaaactt tcttaccgac
      601 caaaactttc ttaccgacca aaatcccctt gatgcctttt acgctatata cccgtccgcc
      661 aaagcggatt tccaaatccg ccgagacttt cgcctctttc ggcgcgctga cggcaagctc
      721 tcggcaatat tcggcaggcg gcggcaggat gagctgctcg ggtttgattt tgttccacgc
      781 ctgatagcgg gtcatgccgt ggcggctgtg tttttgcgta ccgttgtagt aacgcatcca
      841 gcgttccgat aaagcattga gctggtcgat gtcgtgtacc tcggtaaagc gcaacccgct
      901 ctcaaatgcc gtttccacaa tatcgttggc tttttccact tgccccttgg cacgcggatt
      961 gcccggcttg ttgatttgca catgcacatc caagggcttg cacaaatttt taaacgccgc
     1021 cgaagtattc gcgctgcccg ggtcaagcat gaccatgcgc ggtacggtcc ggaacgggtc
     1081 tttgccgatg tcttttttcg cctgcatcat gtagatgaaa aaatcacaga ggttcgcgct
     1141 ggtttcgccg ccgaagtaat aacgcaccga aatcgtgccg gaggcatggt ctgtccccgt
     1201 gtaccgccaa acgcggtcgt tttcgatttt gacgacgttt ttcggcttgt ttttataaaa
     1261 ctcctcttcc ttcatcaccc gcagccccgt atccttgccc tgacgcggca ggaaatacaa
     1321 aacgcacaaa ctcgggtcga tttgccaaca atggttcggg tgttccgatt tcatgcggct
     1381 gacggggtcg ggctgcaaaa gctggtcgga atgcagcttg tattcccgta aagcccgggt
     1441 aatggtgttt tcagaaaggg ggatgacttc cccggtttcc ccgtcaatcc gcgccgcctc
     1501 gattttcccg ttggcgcgca gcatttccac cgcctgccgc accggcatca accgcttgcc
     1561 gttgcgcctc atcgcctccg ccaaaaccgc cgaaatcaat ttggcttctt ccggtttaag
     1621 ctccgtcttg cccgcatcgc tgcgccgttt gcgcgtcggc ttgacgctga ccgcctccag
     1681 cttgcggtat agcgtggcaa ggctgatgcc caattcctgc gcctgctgct taagatatgc
     1741 agagcgtgcg ccgcgcccca ttgcttccgc ctgattttcg actgctttaa gacgctcaat
     1801 cattgccgga ttcatcgcct tctcccgttt caccgcccaa ccattccggc acattgtctg
     1861 ccggtgcttc ggtcggtagg gcatagcttt cgcgcagttg ctcgcagtcc aaaataattt
     1921 gattgagcgt gccgaccatc ttcgcgcggt ggtcaaatcc atgcgcctcg ccgtgcgccg
     1981 ccatctgctc gaacatatcg cgcaatcggc tgatttgcga gcggataccg acttccaagc
     2041 tgccaagctg catcgtcagc tcgatgccca catccgcagg tttaggctct ttgacacccg
     2101 tctgcttttt cgacagcttt tccgccagct catcgacctt tttattttta tcggcaatca
     2161 ccttatcttt cgcttccgcc gtttcacggc tttcgcgcag ggcgacgcgc agctccttaa
     2221 tagtcatacg gtcgacatcg tcaaaagtgt tgccgttgac ctcaccgcct tcggcaagct
     2281 ccaacaaagt gtcgtcgtct tcgaccagca gctcgagcag tttggatttg cccaacgtca
     2341 tcagcttagg ctgcgcttgt ttcattttcg ggtcgataaa acggagggtg gcattcatca
     2401 gtctttggga ttcccgccgt ccgaggccaa ctctttttca gcgatttcgg caaaccgccc
     2461 atgcggcgta tgctctttga tgatgatcag cgcgcgtccc ggctcaaaca tcccttccat
     2521 cgtttggcgt ccgcaaatcg tccgcgctca atccataccg tctcgttgta ggcctcgccg
     2581 cctgaaaact tgtccataac ctccatactg tggatagcca attcgtttgc cgttacgccg
     2641 accgtatgtc ctaaaatttc tgttgccatt tcttttgctc ctcaaatgcg acgtcgtcgt
     2701 cgcatttagt aaattcggtt ttccaattct tgcaagcgcg cattcagccg ctcttgctgt
     2761 tttctgaacc gctctgcgat ttgtagggtt ttgatgccgt aggcgtagtt gccgttttca
     2821 agcttgatga ccaatcccga tgcaaccaaa tcatcaatat ccctgctgac ttgtgatggc
     2881 gtcagcccca ggccgtctga aagctcctta ttgctgatgc cgattaccgg atgcgcgtcc
     2941 atcgccaaaa acacccgcaa cagccgctgc gcctttttac tcgtcgccat ccgcatcctc
     3001 cttcagccca aattttaccg ccgcttcgtg gcttttgccg aaattgcctt tcagcttgcc
     3061 gcgcaagggg tgttccaccg cggtgcgctc caaattgaaa tgtttcgccc ggtgcgtctt
     3121 gcacacgccg ttgcgcttaa accacacggc cgtgctgccg cgcgtttgcg gatagggaat
     3181 aggcttgaaa tttaaaggtt tttccatatt atttatgcct ttcgtgtggt ataatgtgtt
     3241 tagttttagg cctctttgca cgataaacat ccggtctgtt gtgcagcagc agggtcttgg
     3301 cttgttttaa aataaacaag ctttttcccg tagccgggtg tatgtgatcc atcatgagct
     3361 gtcgcgcatg agccttcaaa gtgttcattt tttcgtcctt tttcgtgatg atttagggtg
     3421 tttcatgtct tgatgtgtga attatgagaa aaatatttct cttttcaagg aaatttaagt
     3481 ggaaaaaact tctctttttg gtaagcgatt gaaagaagaa agaattaaat taggcttgaa
     3541 ccaagcagaa gctgctgaaa aatgtggttt ttctcgtgag atgtggggaa agtgggagcg
     3601 tggtgaaaat cgcccatcaa gtgaaaaatt attctctttt tctaaaatcg gcatcgatat
     3661 tgattacgtc atgcacggca gacgcggcga aacagccgcc atgccgtctg aatccttgag
     3721 tgctgaagaa aaagaactgc ttgccctgtt ccgtcagctc ggcagcggca gccgcaaaga
     3781 gcttgctgac tacgccgcct ttaagctggt ggtagagaaa aaagcccaaa ctgcgcttgg
     3841 taaagtgagc aacggataaa aaatgccgtc tgaagatttc agacggcatt tgggacactc
     3901 tgacagtcgg gctgctccgg tgttctgaaa cagatattaa gcaataggag cggccgtgca
     3961 ttttaaagcg tattaaaaac caccccgata ttcaggtcat cggccaagtg gttcagattt
     4021 caaaagacct taattaactt acaataggaa aaatgatgac agaaacgaat tactcttttg
     4081 aagggctggt aattgaaaaa aattattacc catcgtattt atccgaaaaa tgaaaataga
     4141 gagcgtgtag aacctaaaat cagtacacat ttgattgatt tgcctattac ggcaagaaga
     4201 acattggaaa ccaggctgac caaagctttg ggtaataaat ctcatggcat tgaaatgtct
     4261 gttgtcaata cggctgaaaa tagttttttt cagatagcgg cagccataca actcaaagag
     4321 gaagcagagt ttattgagga ttccgcacaa tttgcccaca tgttgaccga tgcacaactg
     4381 aatacaaatg caccgggtgg tattttgttg gttttaaaag gtagggttgg agataccggt
     4441 aagccgtttt tatgtgtaat taaggctgaa cctcaagatg ggttccgaac caaagaagag
     4501 gatgacttta tcacgattga attcttagaa gaattattac tgaccgattc agcaagatta
     4561 ttcaagatag gttttttggt ggctgaaaca gtaaggccgc tagagcaaat acaatctggg
     4621 aattatcgag cttttttgta tgaccatctg atgacacaaa cggaaactag accggcagct
     4681 tcctatttct atcaagtatt cttgggtatg agtatagctg cttcttcccg taaattgacg
     4741 caggattttt ttgagtggac acgcaatttt atcgataact ctgatttaag tgatgatgca
     4801 aaattagatg cgcatgaagc attgcgcgtt acattgaaaa gtgcggaagc aaccattagt
     4861 gtaaataatt ttgcccaaaa tcatttacct caagaaaaac gaacaactta tacagaattt
     4921 atggtggaaa aggattttcc tcaaaatgcc gtaagtaaag atattgaata tattaaaact
     4981 cgtttacgca aacggaggtc ttacggattt agtaacggtg tagttatctt gactcctccc
     5041 gagcatactc aggactatat ggaaattgcg ccaacggaag atggggaata tactgttgtc
     5101 ctaattaaag gacagttaca acaacaaaaa tgagtgatat tgatgatttc aaaagctatt
     5161 tagaaagcca ccaagcggca ttttccgctt ggggtagatt tgtggctgaa gaaattcaaa
     5221 atcaattatc caatgtcatt tctcctgttc cggttgccaa ttttctaaaa attgaagcaa
     5281 agcctggagt caaagagatt tcttcagctc tagccaagat tggccgaaaa aattacactt
     5341 cacctcaaac tcaaatgact gacttggtcg gcgtacgttt tgtggcattg cttgcggaac
     5401 atattcaaat agtttgtgaa atcattgaat cctcatctca gtggaatgct aaagtttcga
     5461 aagattttgc agatgaaatt cagcaaaatc ccaaagcaat ttgactatac aatccaaaca
     5521 ttacgagatt cgtcctaaac aagcattcat tacacccgaa aatgtcagca tccctgctga
     5581 tttgtgctgt gaagttcagg tgcgttcttt actccaacat gcttatgcag agctggttca
     5641 tgacaatata tacaagcctg acggcaatgt tccaaagcaa gcagagcgcg aagtcgcgaa
     5701 gagcatggct ttgatggaaa ctacagatga cttatttagt cgtactctgg caattttgaa
     5761 agaagcaaat caaccacaag aagagcttct tccccaatta tcacagcttt atcaaaagga
     5821 aatagggctt gttcctgaag ttgataaaaa aaccaatatg atttttctag aaacatttca
     5881 atccagcatt tcccaatcaa gtattctttc tgacatccgt tcacttttga atgagaagaa
     5941 atatattgcc aagcggataa aagaaaatgc tgaagaaatg tattttttca gtcagccagc
     6001 agctttgttg gtctattggc tgattgagaa agttggtgca gatgaagtat ggaaaaaatg
     6061 gcctttgcct gcatacaaca aaaacctgaa attcatttgt actgatttgg ataagcaacc
     6121 atcccatgaa ctcttttaac cccctttaaa agcccattca gacggccttt cctaattatg
     6181 ccttaatcaa acgcggcata gcggcagttc cgcagctggc ggaaatcaaa aaatccgcaa
     6241 atcaggaagg gggcggcaat gattgaattt gtccgagcca aaaaacggct gctttgggca
     6301 tttgtacttt tgcttgcgtg ggcgtgcggc taccggtacg ctgccgacaa ggccgaagcg
     6361 gaacaaaccg ccctgattgc cgcctatcgg cattcttcta tggttgcggc ggaacaatac
     6421 gccttgcagc ttaaaaaagc gcaggacgaa aggcagcggt ggtacgactt ttcccaaaaa
     6481 caaagcacgg atttggcggc cgccctgagc gaattggaca aaacgcgcaa caccttacag
     6541 gagtaaaccc gtgaagcggc agacaaagac ggcaaccgtt ttaacggcct tggcacgaac
     6601 agcctgcacc tctacaaccg tgccttcgga taccccgatt aaaaccgttg ccgtcgcgga
     6661 aattccgccc gttccttccg gactgctggt cgaatacgaa cgccccgagc gtccggccgg
     6721 cggctctccc gaacaacttt taaaccacgc cgtccgctat ggcggatact accgaaagct
     6781 cgaaatccaa attgagggct ggcagaactg gcacacgaaa ggccgtctga aacatgactg
     6841 attttgccga ccgcgcatcc gaacgcgaag ccgaatttct ggtggaagcc ttggcgaaac
     6901 accaaccgcc gtctgaaaac accgccggct tcagccattg cgaagactgc ggcgacccga
     6961 taccggaagc aaagcgaaaa gccgtccgag gctgtacgcg ctgtgttgtc tgccacg
//
LOCUS       DAXDXW010000093         6992 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXDXW010000093 DAXDXW010000000
VERSION     DAXDXW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.094"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            118..858
                     /gene="pyrF"
                     /locus_tag="ACFB64_001898"
     CDS             118..858
                     /gene="pyrF"
                     /locus_tag="ACFB64_001898"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC1210200.1"
                     /translation="MNPLITDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPALCQIK
                     IGKELFTATGRSLAESLIHQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGRDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTRAADPVAVLREVNRVANLEAN"
     gene            914..1876
                     /gene="hldA"
                     /locus_tag="ACFB64_001899"
     CDS             914..1876
                     /gene="hldA"
                     /locus_tag="ACFB64_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC1210201.1"
                     /translation="MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGDDEAAAALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKRKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHVGKTVLIDPKGDDYEKYVGATLITPNCAELKEVVGSWKNEGDL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELVEALDGQ"
     gene            1940..2944
                     /gene="rfaD"
                     /locus_tag="ACFB64_001900"
     CDS             1940..2944
                     /gene="rfaD"
                     /locus_tag="ACFB64_001900"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC1210202.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLTKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGCMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKINLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKSELSLKEL
                     VEEELIRYIPFPDALKGKYQGFTQADITKLREAGYKEEFFDVKAGVNRYVKWMLENLA
                     "
     gene            3019..4563
                     /locus_tag="ACFB64_001901"
     CDS             3019..4563
                     /locus_tag="ACFB64_001901"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC1210203.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQGIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGNFEDHRIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSIDWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTCIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEAEDTREVIDIRQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            4679..5530
                     /gene="dinD"
                     /locus_tag="ACFB64_001902"
     CDS             4679..5530
                     /gene="dinD"
                     /locus_tag="ACFB64_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFC1210204.1"
                     /translation="MTTENNAFENAKHIDETGNEYWSARTLQQILEYSEWRNFQRAID
                     KAITACETSGNDKNHHFVETNKMIALGKGGQREVADYRLSRYACYLIVQNGDPSKSVI
                     AAGQTYFAVQARRQELQDEAAFRSLGEDKQRLLLCRQLREHNTDLAAAAKDAGVEKPV
                     EYAVFQNHGYRGLYGGLDKQGIHSRKGLKKSQRILDHMNASEPAANLFRATQTEEKLR
                     RKNIQGKTQANRVHFEVGQKVRQTIEELGGIMPENQPVPEKSIKQLENEEQKRLAATE
                     QHQNGKK"
     gene            5697..6908
                     /locus_tag="ACFB64_001903"
     CDS             5697..6908
                     /locus_tag="ACFB64_001903"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC1210205.1"
                     /translation="MDMQSRAKKLIEMIQTAPVEWKPLGEVLVRTKGTKITAGQMKEM
                     HKDNAPLKIFAGGKTFALVDFDDVPDKDIHREPSIIVKSRGIIEFEYYDKPFSHKNEM
                     WSYHSVNKHIYIKYVYYFLKTQENYFRNIGSKMQMPQIATPDTDNYKIPIPSLETQQK
                     IVKILDKFTELEATLEAELALRKRQYRYYRDLLLDFDNQIGGGIADGYQCRLKNVVWK
                     TLGEVAEYSKNRICSDKLNEHNYVGVDNLLQNREGKKLSGYVPSEGKMTEYIVNDILI
                     GNIRPYLKKIWQADCTGGTNGDVLVIRVTDEKVNPKYLYQVLADDKFFAFNMKHAKGA
                     KMPRGSKAAIMQYKIPIPPLPEQEKIVAILGKFDTLTHSVSEGLPHEIALRRKQYEYY
                     REQLLAFPKAA"
BASE COUNT         2029 a         1911 c         1655 g         1397 t
ORIGIN      
        1 ccttagggaa ccgttccctt tgagccgggg cggggcaacg acgtaccggt ttttgttaat
       61 ccgctatact gcgaaaaaga cgataaaggt cgtctgaaaa cccgaaacga aaacaccatg
      121 aatcctttaa tcaccgactt ccaaactccg caacaacgta cccccgtcat cgttgccctt
      181 gattttgcca acgaaaaaga cacgctcgga ttcgtccgca accttgatcc ggcgttgtgc
      241 caaatcaaaa tcggcaaaga gctgtttacc gcgacgggac gcagtttggc ggaaagcctg
      301 attcatcagg gtttcaaact ctttcttgat ttgaaatacc acgatattcc ccacaccgtc
      361 gctcaggcgt gtaaagtcgc cgccgatatg ggcgtgtgga tggtcgatat gcacgcgtcg
      421 ggcggcagac gtatgatgga agccgccgcc gaagccgttg ccggatacgg cacgaaaccg
      481 ctcttaatcg gcgtaaccgt gttgaccagc atggagcaaa gcgatttggc ggaaatcggt
      541 ttgaacaccg cccctgaaga acaagtcatc cgtttggcaa aactggcaca aagttcgggc
      601 ttggacggcg tggtctgttc cgcccaagaa gccgcgccgc tgcgccgcga attgggacgg
      661 gattttgtct tggttacacc gggtatccgc ttggacgttg ccggcaacaa cgacgaccaa
      721 cgccgcatta tgacacccgc cgaagccttg gcggcgggtt cgacttatct ggtgatgggc
      781 cgccccgtta cccgagccgc cgatccggtc gccgtattgc gcgaagtgaa ccgcgtggca
      841 aaccttgaag caaactgatt ttcaggcggt cttacaggtt aaggttgtct gaacaaaaca
      901 caacggaggc aatatgcccg ccaagttcca acaagaaacc ctcaaatccc gtttcgcaca
      961 agccaaagtt cttgttgtcg gcgacgtgat gctcgaccgc tattggtttg gcgacgtgtc
     1021 ccgaatttcg cccgaagcac ccgtgccggt ggcaaaaatc ggacgaatcg accaacgggc
     1081 gggcggcgcg gcgaacgttg cgcgcaacat cgcttcgttg ggcggcaggg cagggctgtt
     1141 gtccgtaacc ggcgacgacg aagccgccgc cgcgctcgac gcgctgatgg tgcaggacgg
     1201 cgtcgcctcc tatctgatgc gcgacaaaca aatcgccacc accgtcaaac tgcgcgtcgt
     1261 cgcccgcaac cagcagctta tccgccttga ttttgaagaa catcccaacc gcgaagtgtt
     1321 ggaacaaatc aagcggaaat accgcgaaat cttgcccgaa tacgacgcaa tcattttttc
     1381 ggactacggc aaaggcggtc tgtcgcatat ctccgatatg atcgattggg cgaaacatgt
     1441 cggcaaaacc gtcttaatcg accccaaagg cgacgattac gaaaaatatg tcggcgcaac
     1501 tctgattacg cccaactgcg ccgaattgaa agaagtggtt ggcagttgga aaaacgaagg
     1561 cgatctgacc gaaaaagcgc aaaacctgcg ccgccacctc gacttgaccg ccgttttgct
     1621 gacccggagc gaagagggca tgaccttgtt cagcgaaggc gaacccattt accagcccac
     1681 ccgcgcccaa gaagtttacg acgtgtccgg cgcaggcgac accgtcattg ccggaatggg
     1741 cttggggctg gcggcaggct gcaccatgcc cgaagccatg taccttgcca atactgcggc
     1801 cggggttgtc gtggcgaaac tcggtacggc ggtttgctcg tttgcagagt tggttgaagc
     1861 actggacggg caataaatct tttcagacgg catgaccatc caatgccgtc tgaaaccctc
     1921 aaaacaaagg aaaccgaata tgaccatcat cgtaacaggc gcggccggct ttatcggcag
     1981 caacatcgtc aaagccctca accaacgcgg cattaccgac atcgtcgccg tcgataatct
     2041 gacaaaaggc gaaaaattca aaaaccttgc cgagtgcgaa atcgcccact acctcgacaa
     2101 acacgaattt atccgccaag tgagggaaca cattttacct tatcaaaaca tcgaagccgt
     2161 tttccatcaa ggcgcgtgtt ccgatacgat gaaccacgac ggtttgtata tgatggaaaa
     2221 caactaccag tacacgctgg atttgttgga ctggtgtcag gacgaacgca tccccttcct
     2281 ttatgcctcc agtgcggcgg tttacggcaa aggcgaaatc ttccgcgaag agcgcgaact
     2341 cgaaaaaccg cttaatgtgt acggctactc caaattcctg tttgaccaag tattgcgccg
     2401 ccgcatgaaa gaaggtctca ccgcccaagt cgtcggcttc cgctacttca acgtttacgg
     2461 acaacacgaa caacacaaag gctgcatggc atccgtcgcc ttccaccatt tccaccaata
     2521 ccgcgaacac ggttacgtca acctgttcgg cagcaacgac ggctacggca acggcgaaca
     2581 aacccgcgac ttcgtcagcg tcgaagacgt cgccaaaatc aacctctact tcttcgacca
     2641 tcccgaactt tccggcatct acaacctcgg caccggccgc agccaacagt tcaacgaact
     2701 cgccgccgcc accgtcaacg cctgccgcgc cgccgaaggc aaatctgaat tgagcttgaa
     2761 agagttggta gaagaagaac ttatccgcta tatccccttt cccgacgcgc tcaaaggcaa
     2821 ataccagggc ttcacccaag ccgacatcac caaattgcgc gaagccggat ataaggaaga
     2881 atttttcgat gtcaaagcag gtgtcaaccg ctacgtcaaa tggatgctgg aaaatttggc
     2941 ttaatttgaa tgctcgtaaa aaaatcgtct gaaaatatca ggcgattttg atttgtttaa
     3001 ctttatatat ggatttcgat gatgaccgaa atgcaacaac gcgcccaact gcaccgccaa
     3061 atttggaaaa tcgccgacga agtacgcggc gcggtggatg gctgggactt taaacaatac
     3121 gttctcggca cacttttcta ccgctttatc agcgaaaact tcaccgacta tatgcaggcc
     3181 ggcgacagca gcattgatta cgccgctatg ccggacagca tcatcacgcc cgaaatcaaa
     3241 gacgatgccg tcaaagtcaa aggctatttc atctaccccg gccagctttt ttgcaatatt
     3301 gccgccgaag cccatcaaaa cgaagagctc aacaccaagc tgaaagaaat ctttaccgcg
     3361 attgaaagct ccgcctccgg ctacccgtcc gaacaaggca tcaaaggctt gtttgacgac
     3421 ttcgacacca ccagcagccg gctcggcagc accgttgccg acaaaaacaa acgccttgcc
     3481 gccgtcctta aaggcgtggc ggaactcgat ttcggcaatt ttgaagacca ccgcatcgac
     3541 cttttcggtg atgcctacga atacctgatt tccaactacg ccgccaacgc aggcaaatcc
     3601 ggcggcgaat ttttcacccc gcaaagcgtc tccaagctga ttgcgcggct ggcggtgcac
     3661 gggcaggaga aagtcaacaa aatctacgac cccgcctgcg gctcgggcag cctgctcttg
     3721 caggcgaaaa aacagtttga cgaacacatc atcgaagaag gcttcttcgg gcaggaaatc
     3781 aaccacacca cctacaacct cgcccgcatg aatatgtttc tgcacaacgt caattacaac
     3841 aaattccaca tcgaattggg cgacacgctg accaacccca aactcaaaga cagcaaaccc
     3901 tttgatgccg tcgtctccaa tccgccctat tccatcgact ggataggcag cgacgacccc
     3961 accttgatca acgacgaccg ctttgccccc gcaggcgtac tcgcaccgaa atccaaagcc
     4021 gattttgcct tcatcctgca cgcactgaac tacctttccg gcagaggccg cgccgctatc
     4081 gtctcattcc ccggcatttt ctatcgcggc ggcgcagagc agaaaatccg ccaatatctg
     4141 gtggagggca actatgtgga aaccgtgatt gcccttgcgc ccaatctctt ttacggcacc
     4201 tgcatcgccg tcaatatcct ggttttgtcc aaacacaaag acaataccga catccaattc
     4261 atcgatgcag gcggcttctt taaaaaagaa accaacaaca acgtcttaac cgaagaacac
     4321 attgccgaaa tcgtcaaact cttcgccgac aaagccgatg tgccgcatat cgcccaaaac
     4381 gccgcccagc aaaccgtcaa agacaacggc tacaacctcg ccgtcagcag ctatgtcgaa
     4441 gccgaagaca cccgcgaggt catcgacatc agacagctta acgccgaaat cagcgaaacc
     4501 gtcgccaaaa tcgaacggct gcggcgtgaa attgacgaag tgattgcaga gattgaggcc
     4561 taggcagttg ttgcatattt tgcaacaact gaatgggaaa gaggccgtct gaaaatacgg
     4621 gcggcaacct gtaagcaatc cttacaagtt caaatcaaga aattttcggg gaaaatacat
     4681 gacaacagaa aataacgctt ttgaaaacgc caaacacatc gacgaaacag gcaatgaata
     4741 ttggtcggcg cgcaccttgc agcaaatctt ggaatattcc gaatggcgca atttccaacg
     4801 ggccattgat aaagccataa ccgcttgcga aacatcagga aatgacaaaa atcatcattt
     4861 tgttgaaacc aacaaaatga tagcccttgg gaaaggcggg cagcgcgaag tggcggatta
     4921 ccgcctttcc cgctatgcct gctacctgat tgtgcaaaac ggcgatccgt ccaaaagcgt
     4981 cattgccgca gggcaaacct actttgccgt gcaagcccgc cgccaagagc tgcaagacga
     5041 ggcagcattc aggagtttgg gcgaagacaa acaacgcctg ctgctttgca ggcagctgcg
     5101 cgaacacaat accgaccttg ccgccgcagc caaggatgca ggcgtagaaa aacccgttga
     5161 gtacgccgtc ttccaaaacc acggctatcg ggggctatac ggaggactgg acaagcaggg
     5221 catacacagc cgcaaaggct tgaagaaaag ccagagaatc ctagaccaca tgaacgccag
     5281 cgaaccggct gccaatctgt ttcgtgccac gcaaaccgaa gaaaaactac gccgcaaaaa
     5341 catccaaggc aaaacacagg caaaccgggt gcatttcgaa gtcggacaaa aagtgcgcca
     5401 aaccattgaa gaactgggcg gcatcatgcc tgaaaaccag cccgtacccg agaaaagcat
     5461 caaacagctt gagaacgaag aacagaaaag gcttgccgca accgaacagc atcaaaacgg
     5521 caaaaaataa ccgcattcag gctacctgaa aaagcagcct gcacacgatt tcaggcagcc
     5581 tgaaagcagg acgggcttca gcccgcagaa acaacggcaa acggacatag cgggctgaaa
     5641 cccgccccgc aaccgcccca tatcccgccg cagcgggaag gaaacggaaa acaaccatgg
     5701 atatgcaaag cagagcgaaa aaattgattg agatgattca gacggcaccg gtggagtgga
     5761 agccattggg ggaggtttta gttcgtacaa aaggaactaa gataactgct ggacaaatga
     5821 aagaaatgca taaagataat gcgccgttaa agatttttgc aggaggaaaa acttttgcat
     5881 tagttgactt tgatgatgtt cctgataagg atatccatag agagccttct attatcgtta
     5941 aatcaagagg tattattgaa ttcgaatatt acgataaacc attttcgcat aaaaatgaaa
     6001 tgtggtctta tcattcagtt aataaacata tttatataaa atatgtttat tatttcttaa
     6061 aaacacaaga aaactatttt cgtaatatcg gatcaaaaat gcaaatgcca caaatagcaa
     6121 caccagatac agataactat aaaatcccca tcccatcatt ggaaacccaa caaaaaattg
     6181 taaaaatact tgacaaattc accgagctgg aagctacgct ggaagcggaa ttagccctgc
     6241 gcaaacgcca ataccggtat taccgcgact tacttttaga ttttgacaat caaatcgggg
     6301 gggggatagc tgacggctat caatgccgtc tgaaaaatgt ggtttggaag acgttggggg
     6361 aggttgccga atattcaaaa aaccgtattt gttcggataa actgaacgaa cataattacg
     6421 ttggcgtgga taatctctta caaaatagag aaggtaaaaa gttatccggt tatgttccaa
     6481 gcgaaggaaa aatgacagaa tatattgtca atgacatttt gattggaaat atccgtccgt
     6541 atttgaaaaa aatctggcag gcagactgca cgggcggaac aaacggtgat gttttggtta
     6601 tccgggtaac agatgaaaag gttaatccaa aatatctgta tcaggtattg gccgatgata
     6661 aattttttgc ttttaatatg aagcacgcca aaggtgcaaa aatgccgcgt ggcagcaaag
     6721 cagcgattat gcaatataaa atccccatcc ccccgctccc cgaacaggaa aaaatcgtcg
     6781 ccatcctagg caaattcgat accctgaccc actccgtcag cgaaggcctg ccgcacgaaa
     6841 tcgccctgcg ccgcaaacaa tacgaatatt accgcgaaca gctgcttgcc ttccccaagg
     6901 ctgcctgaaa aagcagcctg cactttttca ggcagcccaa tccgaacatc aaaccgttta
     6961 tagtgaatta acaaaaatca ggacaaggcg gc
//
LOCUS       DAXDXW010000094         6990 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXDXW010000094 DAXDXW010000000
VERSION     DAXDXW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.095"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..76
                     /locus_tag="ACFB64_001904"
     CDS             <1..76
                     /locus_tag="ACFB64_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC1210206.1"
                     /translation="GWEFRLNSGTPPRQLKILRDRCGI"
     gene            131..343
                     /locus_tag="ACFB64_001905"
     CDS             131..343
                     /locus_tag="ACFB64_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC1210207.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            528..1028
                     /locus_tag="ACFB64_001906"
                     /pseudo
     CDS             528..1028
                     /locus_tag="ACFB64_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
     gene            1025..1351
                     /locus_tag="ACFB64_001907"
     CDS             1025..1351
                     /locus_tag="ACFB64_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFC1210208.1"
                     /translation="MTDAENGRGQDTLAHIAQSIRNILFTRIGTRLMREEYGSFIPDL
                     IDMPAGHAAIALIHQAAVTALARWKPRITVRRIQADTADLAAGKIKLTLDVTLADGGE
                     RTYRIK"
     gene            1612..2082
                     /locus_tag="ACFB64_001908"
                     /pseudo
     CDS             1612..2082
                     /locus_tag="ACFB64_001908"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            2258..2821
                     /locus_tag="ACFB64_001909"
     CDS             2258..2821
                     /locus_tag="ACFB64_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFC1210209.1"
                     /translation="MNSTVPANNSPLQHALAKLTERETAAVSRQLDPARCDPGFLPFH
                     AFARSIGTEEGWDFAETDEARRNLIAGFAEIHARKGTPYAIRALFPILRLGEIQIIER
                     DGEFKWDGSVLFDGSRTFGRREGDWAEYRIVLTRPVSIRQTARIRAMLAEIAPLRCEL
                     TALDYRNHPHRWNGKIRFNGEYGFGTT"
     gene            2861..5112
                     /locus_tag="ACFB64_001910"
                     /pseudo
     CDS             2861..5112
                     /locus_tag="ACFB64_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238084.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            5211..5540
                     /locus_tag="ACFB64_001911"
     CDS             5211..5540
                     /locus_tag="ACFB64_001911"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210210.1"
                     /translation="MPRQSCHSSPFPRKRESGTQTRQESIGKNNPTAVIPAQAGIQTH
                     NVKAVYQKKPKPNALDFRLRGNDEELGSDERREQPRKKPPTPSDGIGNKNRTAETARE
                     RIAGRAR"
     gene            complement(5556..5867)
                     /locus_tag="ACFB64_001912"
     CDS             complement(5556..5867)
                     /locus_tag="ACFB64_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFC1210211.1"
                     /translation="MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQS
                     SITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQRKTFDIAPLFPEPPLSSMKTGAR
                     T"
     gene            complement(5875..6154)
                     /locus_tag="ACFB64_001913"
                     /pseudo
     CDS             complement(5875..6154)
                     /locus_tag="ACFB64_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6239..6852
                     /locus_tag="ACFB64_001914"
                     /pseudo
     CDS             6239..6852
                     /locus_tag="ACFB64_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6831..>6990
                     /locus_tag="ACFB64_001915"
     CDS             6831..>6990
                     /locus_tag="ACFB64_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HFC1210212.1"
                     /translation="MGAKRRLREYIYHLLVAIDQLFNAITGGAADETLSSRTYRGARL
                     AQQPKTRWK"
BASE COUNT         1888 a         2387 c         1681 g         1034 t
ORIGIN      
        1 aggatgggaa tttcgactta actccggcac accgccccgg cagctaaaaa tcctgcggga
       61 tcggtgtgga atttagggat aatctagtac agccacaaaa aatatgcgaa acaaaataaa
      121 ccgcaacgac atggaactcg gctacacgcc ttacaacctt cgcaccctgc gcaaccgctg
      181 caaactgaca caggccgagt tggcgcaaat cgtcggggtg aagcactaca tacaggtagg
      241 ccgctgggaa gccgaacccg ataccgaaac gcggcgggcc gatatgccgc tggaaaaatg
      301 gcggcagttc ctcgattgga tagaaaaaac aaacgccgtc tgaaaccttc agacggcatt
      361 ttttacccac ccccaaacct aaaaaccaca accaagccct acaccgcttg cccccacctc
      421 gccgccgccc caaaataacc tcactctgtt ggaagcctgc cggaaacggc aaaagccgcc
      481 ccgttccgcc attacggggc ggtttcccac atcaagacga gacagaaatg accgaccgcc
      541 aaatagacaa cctcatcaaa cccgccacca tcgccgacac cgaccccgcg tccgcgcaca
      601 acacggcggc attaccaccg actggctgcc ctatatcgtc ccctttgcag gcggcgtgtc
      661 agtatggcgc atcccaagtg tcggcgaagc ctgcaccatc ttatcccctg ccggcgaacc
      721 cgagaacggc gtagttttat gctgccaagc ctccgaccga taccccgccc cgtccgccga
      781 ccccgccgaa accgtcgtcc gatttcatcc gctacaacca caacagcggc gcgatggaat
      841 taaaagccgt tacaagcctg accatcgaca ccccccaaac caccataacc ggacacctga
      901 ccgtcaacca aaccaccacc gcccaagggc tgctgaccta ccaaaacggc atgaacggcc
      961 aaggcggcag cctgtccgag cacacccacc ccgacgactc cggcggtaca acggaaaaac
     1021 cccaatgacc gacgcagaaa acggacgcgg gcaagacacc ctcgcccaca tcgcccaatc
     1081 catccgcaac atcctgttca caagaatagg aacgcggctg atgcgcgaag aatacggcag
     1141 ttttattccc gacttgatcg atatgcccgc cggtcacgcc gccatcgccc tcatccacca
     1201 agccgccgtt accgccctcg caagatggaa accccgcata accgtccgcc gcatacaggc
     1261 agacaccgcc gacctcgccg ccggcaaaat caaattaacc ctagacgtaa cccttgccga
     1321 cggcggcgaa cgaacctacc gcatcaaata ataaaaacaa caggataaac aatgggaaac
     1381 agccgattaa gccaactccc cgcgcccgcc gccatcgaag aaaccgactt tgagggcatc
     1441 ttcgcgcgca aaaaagccgc cctcaccgcc ctatgtcccg aaagcatccg cgaaaccgtc
     1501 gcccaaaccc tcgaattaga atcagaaccc ctaaccatcg acctgcaaca gcaagcctat
     1561 caagagctgc tcgtccgcaa ccgcatcaac gaagccgtca aagccaacct cttggcatac
     1621 gcacaaggca gcgacctcga ccacatcgcc gcccaatacg gactttcacg caaaaccatc
     1681 cgcgccgccg accccgacgc gaacccgccg gttgccgccg aatacgaaac cgacgacgca
     1741 ttccgcgccc gcgtccaagc ccatcccgaa aaatacgccg ccgggccgcg caccgcatac
     1801 gaagcccacg ccatcgacgc acacccccaa atcacacacg cccgcgccgt gcgccgcgcc
     1861 gtcggcacgg tggaggttta catcaaaacc caaagcggca cgcccgacga aaccattttg
     1921 accgccgcgc gcgaatacct gcccgccgaa acacgccgcc ccctttgcga caacgtgcaa
     1981 gtaaccgccg cccaacccaa agacgcggca gtagagtatt ccgccgaata ccaccccgcc
     2041 gccaatattc aagccgaacg gcagaccgcc tgcgaagcct tggataacct atggcggcaa
     2101 aacgcccaca tcggcgcatc cgtcgccctg tctaaaatca tcggcgcgtt agacaccccc
     2161 ggcgtgaaaa aaaatcacac tgcacagccc cgctgccgac atcgaatgcg gcaacggcga
     2221 atacatccga atcacgtcca cgctcgagcg cgaataaatg aacagcaccg tccccgcgaa
     2281 caacagcccc ctgcaacacg cactggcaaa gctgacagaa cgcgaaactg ccgccgtctc
     2341 ccgccaactc gaccccgccc gatgcgaccc cggattttta ccctttcacg ccttcgcaag
     2401 aagcatcggc acggaagagg gctgggactt tgccgaaacc gacgaagccc gccgcaacct
     2461 catcgcaggc tttgccgaaa tccacgcccg aaaaggcacg ccgtacgcca tccgcgccct
     2521 cttccccatc ttgcggctgg gcgaaatcca aattatcgaa cgcgacggcg agttcaagtg
     2581 ggacggctcg gtcttgttcg acggcagccg cacattcggc aggcgcgagg gtgactgggc
     2641 ggaataccgc attgtcttaa cgcgccccgt cagcatccgc caaaccgccc gcatccgcgc
     2701 catgttggcg gaaatcgccc ccttgcggtg cgaacttacc gcgctcgact accgcaacca
     2761 tccccaccgc tggaacggca aaatccgctt taacggcgaa tacggtttcg gcacgacata
     2821 acgcgccccc gaaaatcaac aaacaaaagg aagccccaaa atggcaaacg caaccgaaca
     2881 aaaccaattc gaccaagccg tccgcctcat cgaacccggc gacagcgtcg tcgtcggccc
     2941 aggcgcgccc gtcaaccaac cactgcaagc cctcgccaac cgcacccttt tgctcaaaaa
     3001 ccaaaccgaa gcccttcaga cggcatccga caccaaagcc gccgcaagca ccgccgtcaa
     3061 cgcaggcgac ggactgacgg gcggcggcag ccttgcccaa agccgcacca tcgcactggg
     3121 cgcgcccggg caaatcaccg caacaagcca aaacaccgtc ccaaaaaacg gccacaccca
     3181 cgccatcgac accgcacgca ccgaccgcgc cggcatcgtc cgattagaca atgccattag
     3241 cgaagccgaa gacaccgccg ccacccccaa agccgtcaaa accgcgctcg accaagcccg
     3301 cgccgccgcc gcgaccgccg acctcaaagt ctccctctcc gacaaccaaa ccgttaccgg
     3361 ccaaaaaacc tttaccgccg aaacacaatt ccaaagcggc atccggctgt ccgccaaccc
     3421 gacgcactga aacggcggct acaaagccta tatcggcgcg gataacgaaa acgcccacat
     3481 cgtcttcggc gacgacaccc tgcgcctgca cggcgcaaat aaccgcattt cctacaacaa
     3541 ccacgacatc ttccacaaag ccaacaaacc gcgtttcaac gaagacatcg aaggcaaacc
     3601 gaacacgctg tccggctacg gcatcggcaa cttcaaagtc gaaacattcc ggggcgattt
     3661 gaacagcctc aaaaccgacg gcgtctattc cctgccgacg gcggtcggca gctcgaacct
     3721 gcccgttgaa aacaccgcct gccatatcca agtcatcgcc ggcacgcaac ccggctggtg
     3781 caggcagttg ggctatcccg cctacacatc cgacgtgtac gaacgctacc agcccagcag
     3841 cgcaaacgac gactggagcg cgtggaaaaa actcaattcg gaaggcatcc ccgccggcgc
     3901 gatcgtgtcc tttcccaaag ccgtccgaaa ccccgcaggc tatctcaaag ccgacggcac
     3961 gatctttgca caaaacacct tctcgacctt taccgcgccc tgggcaacac aaacaaactg
     4021 cccgacctga cccgtaccga catcggcatc accgcgtggt ttccgtccga ccaaatccag
     4081 acgggctggc tggcgtttga cgacatccga gagcgcgtaa ccgaatcggc ttatcccgaa
     4141 ctttaccgcc tgctgaccga aaaatacgga cgcatccaaa acgtcccgca ggcggaagac
     4201 cgctttatcc gcaacgcggg caacggcttg gcggtcggaa cgaagcagga agacgaaatc
     4261 aaacggcacg tccacaaagt attttcacac tgggcaaacc acccagacgc cgccgccgtc
     4321 ggttacgaag accgcaatga aaggcaaaga agcgcgctcg tatcgacttg gacggacggc
     4381 gacttaaacg acaacggctt tttaaccccg cgattggaca gcaaaatggc aacgggcggc
     4441 gcagaaaacc gccccaaagc cctggtttta aaactgtgca tcaaagccgc cgacaccttg
     4501 ggcgaagccg tgttccggat aaagtcccac ggcgaaaccg ccaacgccgg cgcactggac
     4561 gcgtcaaggc tggcgcaagg tttgcaggaa aaagccgacc gcgaccacac ccacaccacc
     4621 gcccaaatcc aagggctgga cgaaaaaatc agcgccgccg ttgccgcgca attcacacgc
     4681 caaaccatcg gcggcgtgga tattgtcaga ttccccgacg gcacaatgat acagaccggc
     4741 agttacaggt ttgcaagagg cggcagcccc ataggaaacg aagttgtctt ccccatcgcc
     4801 tttgccgacg gcaacgtcaa atgcttcgta tccgaacgcc attcgggacg cgtcaacggc
     4861 gaaaggcaac acaactggct gtttatccgc gcaaaaaacc acgccgccgc cattattacc
     4921 aactggtacg aaagcagttg cgactggatg gccatcggca aatccgcctc gggaaacgcc
     4981 gccagcccca ccccgatagt ccccgaaata cctgaaatcg atgaagaacc gcaaagagag
     5041 agtggaagat catcaaccgg actccgaaac ccccgccgcc accgaggcct cgacttcccc
     5101 gtggggtcgt aagactagcg ggcggcaggg caggctgtag agacgggctt cagcccaccg
     5161 atccaagcaa tccgaccgaa accggccgcg ccgccaatcc tgcgaaacct atgccccgcc
     5221 aatcctgcca ctcttcgcca ttcccgcgaa agcgggaatc cggaacccaa acgcggcagg
     5281 aatctatcgg aaagaataac ccgaccgccg tcattcccgc gcaggctgga atccagaccc
     5341 acaacgttaa ggcggtttat cagaaaaaac cgaaaccgaa cgccctagat ttccgcctgc
     5401 gcgggaatga cgaagagttg gggagtgacg aaaggcggga acaaccgcgc aaaaagccgc
     5461 cgactccttc agacggcatc ggcaataaaa accgcacggc cgaaaccgcg cgggaaagga
     5521 tagccgggcg cgcccgataa gcggcggccg cagggttaag tccttgcacc tgttttcatc
     5581 gaagacaaag gcggttcggg aaataagggc gcaatatcaa aagttttgcg ttgcctgatt
     5641 tgccacaaat cataaccggc ctgcatattc agccaaaaat caggcgaagt gttgagcagt
     5701 tggcttaaac gaaccgccat ctcgggcgta atcgaagatt gggcgttgac aatgcgcgat
     5761 aaggcaaccc ggctgacacc caggcgtttc gctatatcgg taatgcttgc gccgttaata
     5821 tactctttta aaacaaggcc cgggtgggca ggattgtgca ttttcatatt attcctagtg
     5881 ataatcctga taatcgacaa cttcgacatt gccgtcgttg aaacgaaaag tcaaacgcca
     5941 atttccgttg actttgaacc gcccaatgtc ccgacaaact gcccttgagg ggatgaagat
     6001 tccaactcgg cacagccata tccgaaggcg tttgcgcggc attcagcgcg gttagaagca
     6061 gattcagttt gacggaatgc ccggcttgga tgcccgatag cgagcctgtt ttaaaaaagc
     6121 gttcaagccc tttgtgtttg aaagaaacaa tcatcatccc accccgtctg tatcgccaaa
     6181 ggatacaaaa cttttatttg attgcaaagc gacgatatac aaaaaaggaa accccgaaat
     6241 gaccatctat ttcaaaaacg gcttttacga cgacacattg ggcagcgtcc ccgaaggcgc
     6301 ggttgccgtc ggcggcgaag aatacgccgc cctcttcgca ggacaggcgc agggcgggca
     6361 aattgccgcc gattccgacg gccgccccgt tttaaccccg ccgcgcccgt ccgagtacca
     6421 cgaatgggac ggcaaaaaat ggaaaatcag caaagccgcc gcccgtttcg ccaactcccg
     6481 tttcgccaaa caaaaaaccg ccttggcatt cagcctcgcg gaaaaggcgg acgaactcaa
     6541 aacagcctct tggcgggcta tccccaagtg gaaatcgaca gcttttacag gcaggaaaaa
     6601 gaaaccctcg cgtggcaggc ggacaacgac gccccgaccc cgatgctggc gcaaatcgcc
     6661 gccgcaaggg gcatagaatt ggacgttttg attggaaagg ttgtcgaaaa acccgcccgc
     6721 ctggccgttg ccgtcgacgc gattatcgga aagcggcagc aacttgaaga tagattaaat
     6781 accatcgaaa ccgcgccagc attagatgcg ttagaaaggg aaatcaaaga atgggcgcta
     6841 aacgtcggct gagagaatat atttatcatc tactagtggc catagaccaa ctgttcaacg
     6901 ctataaccgg cggcgcggca gacgaaaccc tctcaagtcg cacctatcgc ggcgcgcggc
     6961 tcgcccaaca acccaaaacc cgctggaaga
//
LOCUS       DAXDXW010000095         6858 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXDXW010000095 DAXDXW010000000
VERSION     DAXDXW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.096"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(954..1637)
                     /locus_tag="ACFB64_001916"
     CDS             complement(954..1637)
                     /locus_tag="ACFB64_001916"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC1210213.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDAFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1786..4089
                     /gene="parC"
                     /locus_tag="ACFB64_001917"
     CDS             1786..4089
                     /gene="parC"
                     /locus_tag="ACFB64_001917"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC1210214.1"
                     /translation="MNTQPHASHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDRSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFMPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDRLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRVVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAVFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTAADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYSIDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRLPV
                     ALIEAKRGKKGRLLPISGSLKQLSSPK"
     gene            4109..5833
                     /locus_tag="ACFB64_001918"
     CDS             4109..5833
                     /locus_tag="ACFB64_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HFC1210215.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYLWLAIACWLIFFSTLNPQWQWQWQALRIPSFSAVADITMIGVLTY
                     LFGGIDSGFGILILPFVGSSCLLSYGRYPLLYASYASILLIFNALADSNINMYPLILD
                     AKTVTNTFVVVAGSYFVAMIASLSVRYIDRAGKLAHENHVAYRRIRGLNQIVLNRVQE
                     AVVVINVEHQTILFNKKAKDLLPMLEIGQHTSLFDPVAILWDKTSSRTFEHYIDTPEL
                     TARIRAVPMNKKQNKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIR
                     HADGGTLFLDEVADLPLSMQVKLLRAIQEKAVRRIGDATEQPVDVRIVCATHKNLEAL
                     VESGAFRQDLYYRLNVVSLNMPSLREMRENLKLPTPYLLYKHSHNNRPYTLSPAAQQM
                     LLNYSYPGNFRELENILERAVALCVGYTVQIDDLQIQDVHHKPVRTETAVPVADTLPS
                     ETAAAPSRLLPFDPDTMQIQDYLDKIERDIIGQVLKQTEGNRTQAAKRLGISFRSMRY
                     RMERLNID"
     gene            5933..6685
                     /locus_tag="ACFB64_001919"
     CDS             5933..6685
                     /locus_tag="ACFB64_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC1210216.1"
                     /translation="MTDILIDDTATEAVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSNVIVDFTSGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAEQMPALVKTQG
                     KPDIVYLDPMYPERRKSAAVKKEMAYFHRLVGEAQDEVILLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGADKG"
BASE COUNT         1642 a         2046 c         1685 g         1485 t
ORIGIN      
        1 tattttagta gttttaccat tagaaaaagg atatgaatgg aattgccttg aggtagaagt
       61 actatccgct gctcaatctc tagcaaatat ctattgcaac aaggttcgcc tcatagatgg
      121 tcatgtttgg gaggaataaa tgaaaaagcg tagttggtaa tttttagttg cgcagggtgg
      181 gcttcagccc accgtttttt cttcattttg ggttgttgat tggtgggcta aagcccaccc
      241 tacgattact aaaatcccgt cattcccgcg tagagcgcat catataaggc gggaatccag
      301 acctttcggt gcgaaaactt atcggataaa acggtttccc ctgcatcctg gattcccact
      361 ttcgtgggaa tgacgggata taggtttccc tacggacgtg ttcagattcc tgccttcgcg
      421 ggaataacgg cggagcagtt tctgcttttc caataaatga ccgtaactta aaatcccgtc
      481 atccccgcga aagcaaaaat cctgcctgtc ggatttcggt tttttcggga aactgatgaa
      541 tcgtcattcc cgcgcaggcg ggaatccaga acgcgggacg gcggcaatat tcaaaggttg
      601 tctgaaaatt cagaggttcc agattcccgc cttcgcggga atgacagcgg agcagtttct
      661 gcttttccga taaatgaccg caacctaaaa cccatccttc acacgaaaac agaaaaacaa
      721 aaacctaaaa tcccgtcatt cccgcgataa cagttgcgta attgcgtagg gtgggcttca
      781 gcccaccgtt tcttcatttt tgcttgtcaa ttagtggact gaagcccacc ctgcggttac
      841 tgtttcttct ttttcgatta ttaattggca ggctgaagcc ctccctacgt ttaccgtcaa
      901 aaatgccgtc tgaaatgctc aagccctcag acggcatttt ttatttcgca cccttacagg
      961 taaaatttct cgatgacttt caggttgtcg tctaatttgt acaccagcgg ctggccggtc
     1021 gggatttcca agcccatgat gtcttcgtcg gaaatgccct cgatgtgttt tgccagcgcg
     1081 cgcagggagt tgccgtgcgc cgctaccaag acgcgtttgc cgctcaaaat cgcgggggcg
     1141 atttggtctt cccaaaacgg taatacgcgc tccagcgtta ctttcaggtt ttcgccgtcg
     1201 ggtacgacat cggcaggcag atgggcatag cgacggtctt tgtgtgcgga aaacgcgtcg
     1261 tctttgtcca aaagcggcgg cagggtgtcg tagctgcgcc gccagatgcg gacttgctcg
     1321 tcgccgtatt tttcggcggt ttgttttttg tccaaacctt gcagtcggcc gtagtggcgt
     1381 tcgttcagcc tccatgtttt gatttgcggt acgaacagtt ggtcggattc ttccaaaacg
     1441 atgttgcagg tcttaatcgc gcgggtcagg acggatgtga aggcgatgtc gaactcatag
     1501 ccgttttctt tcagtttttt accggcggcg gcggcctcgg caagcccctg ctcgctcagc
     1561 ttcacgtcgc gccagcctgt aaacaggttt ttcgcgttcc attcgctttg tccgtggcgg
     1621 ataaatacca attccatatc gtctccaatg tgtgaaagtg ggaaagcttt atttataaca
     1681 tattttcaca tttcccgtat ttgattcaga ttcaggcacg gcgcccacta tggtttgcca
     1741 ttttgattta caataatgtc ctttgcttta cattccgcat acacaatgaa tacgcaaccg
     1801 cacgcttccc ataccgattc caacacgctg atgctcggcc gatacgccga acgcgcctat
     1861 ctcgaatacg ccatgagcgt ggtcaaaggc cgcgcgctgc ctgaagtttc agacggccaa
     1921 aagcccgtgc agcggcgcat tttgtttgcc atgcgcgata tgggtttgac ggcgggggcg
     1981 aagccggtga aatcggcgcg cgtggtcggc gagattttgg gtaaatacca tccgcacggc
     2041 gaccgttccg cctatgaggc gatggtgcgc atggctcagg attttacctt gcgctacccc
     2101 ttaatcgacg gcatcggcaa cttcggttcg cgcgacggcg acggggcggc ggcgatgcgt
     2161 tacaccgaag cgcggctgac gccgattgcg gaattgctgt tgtccgaaat caatcagggg
     2221 acggtggatt ttatgccgaa ctacgacggc gcgtttgacg agccgctgca ccttcccgcc
     2281 cgcttgccta tggtgttgct caacggcgcg tcgggcatcg cggtgggtat ggcgaccgag
     2341 attccgtcgc acaatttgaa cgaagtcacg caggcggcga ttgcactgtt gaagaaaccg
     2401 acgctggaaa ccgccgacct gatgcaatat attcctgctc ccgattttgc cggcggcggt
     2461 caaatcatca cgccggcgga cgaattgcgc cgtatttacg aaaccggcaa gggcagcgtg
     2521 cgcgtgcgtg cgcgttatga aatcgagaaa ttggcgcgcg gacagtggcg cgtcatcgta
     2581 accgaactgc cgccgaacgc caattccgcc aaaatccttg ccgaaatcga agagcaaacc
     2641 aacccgaaac cgaaagcggg taaaaagcag ctcaaccaag accggctcaa taccaaaaag
     2701 ctgatgctgg atttaatcga ccgcgtgcgc gacgagtccg acggcgaaca tcccgtgcgc
     2761 cttgtatttg aacccaaatc cagccgcatc gataccgata ccttcatcaa cacgctgatg
     2821 gcgcaaactt cgctggaagg caatgtgtcc atgaacttgg tgatgatggg tttggacaac
     2881 cgccccgcgc agaaaaacct gaaaacgatt ttgcaggaat ggctggattt ccgcgtcgtt
     2941 accgtaacac gccgtctgaa attccgttta aaccaagtgg aaaaacggct gcacatcctc
     3001 gaaggccgtc tgaaagtctt tctgcacatc gacgaagtga ttaaagtcat ccgcgaatcg
     3061 gacgacccga aagccgattt gatggcggtg ttcgggctga ccgaaatcca agccgaagac
     3121 attttggaaa tccgcctgcg tcagctggcg cgtttggaag gtttcaaact cgaaaaagaa
     3181 ttgaacgaat tgcgcgaaga acaaggccgt ctgaacatcc ttttgggcga cgaaaacgaa
     3241 aaacgcaagc tgattatcaa agagatgcag gcggacatga agcagttcgg cgacgcgcgc
     3301 cgcacgctgg tggaagaggc cggacgcgcc gtgctgacac aaaccgccgc cgacgaaccc
     3361 atcacgctga ttttgtcgga aaaaggctgg atacgcagcc gtgccggaca taatctcgat
     3421 ttgagccaaa ccgcgttcaa agaaggcgac cgcctcaaac aaacccttga aggccgcact
     3481 gttttacccg tcgtcatcct cgattcatcg ggcagaacct actcgatcga tgccgccgaa
     3541 atccccggcg gacgcggcga cggcgtaccg gtttcctcct taatcgagtt gcaaaacggc
     3601 gcgaaacccg tcgcgatgtt gacaggattg ccggaacaac attatttatt atcaagcagc
     3661 ggcggctacg gctttatcgc caagctgggc gatatggtcg gacgcgtgaa agcgggcaaa
     3721 gtggtgatga ccgcagacag cggcgaaacc gtcctgccgc cggttgccgt ctatgcctcc
     3781 tcgttcatca accccgactg caaaatcatt gcagccacca gtcaaaaccg cgccctcgcc
     3841 ttccccatcg gcgaattgaa aattatggcg aaaggcaaag gactgcaaat catcggatta
     3901 aacgccggcg aatcgatgac gcataccgcc gtttcttccg agccggaaat cctgattgaa
     3961 agcgaaggca ggcgcggcgc ggcgcacaaa gaccgcctcc ccgtcgccct gattgaggca
     4021 aaacgcggca aaaaaggcag actgttgccc atatcgggca gcctgaaaca gctttcttcc
     4081 cccaaataaa cccggttccg cacatattat ggtgatttcc aacccccgcg aacttgaaaa
     4141 actcaaagac cggattccca atctgatcaa catcatccgc gtcgccatcg ttttcccgct
     4201 gatgattatg cacatcctcg ggctggaaac cggcagccgt gcgaacctgc acgcctcatg
     4261 gacggcgtgg gcgttttact tgtggcttgc cattgcctgc tggctgattt tcttttccac
     4321 cctcaacccg caatggcaat ggcaatggca ggctttgaga ataccgagtt tcagcgccgt
     4381 ggccgacatc acgatgatcg gcgtgctgac ctacctgttc ggcggcatcg attcggggtt
     4441 cggcatcctg atcctgccct tcgtgggcag ctcctgccta ctcagttacg ggcgttatcc
     4501 cctgctctat gccagctatg catccatcct gctgatattc aacgcccttg cagacagcaa
     4561 tatcaatatg tatccgctga ttctggatgc aaaaaccgtc accaatacct tcgtcgtcgt
     4621 agccggctcc tatttcgtcg ctatgattgc ctcgctgtcg gtcagataca tcgaccgtgc
     4681 cggcaaactt gcccatgaaa accacgtcgc ctaccgccgt atcaggggct tgaaccaaat
     4741 cgtgctcaac cgcgttcagg aagcggtcgt cgtcatcaac gtcgagcatc agaccatact
     4801 gttcaataaa aaggcaaaag atctgctccc catgcttgaa atcggacagc atacctccct
     4861 gttcgaccct gtcgccatct tatgggataa aacatcctca cgcaccttcg aacactatat
     4921 cgacacgccc gaactgaccg cccgcatccg cgccgtgccg atgaacaaaa agcagaacaa
     4981 gctgctcatc ctctatatcc gcccgcaaag cgaaattcag gcggaagccc tgtccgtcaa
     5041 acttgccgcg ctcggacaac tgaccgccaa ccttgcccac gaaatccgca acccgatgtc
     5101 cgccatccgc cacgccgacg gcggcacttt atttttggac gaagtggccg acctgcccct
     5161 ttccatgcag gtgaaactct tgcgcgcgat tcaagaaaaa gccgtgcgcc gtatcggcga
     5221 cgcgaccgag cagcccgtcg atgtccgcat cgtctgcgcc acccacaaaa acctcgaagc
     5281 ccttgtcgaa agcggcgcat tccgccaaga cctgtattac cgtctcaatg tcgtcagcct
     5341 caatatgccg tccctgcgtg aaatgcgcga aaatttgaag ctgcccaccc cctacctcct
     5401 gtacaaacac agccacaaca accggcccta cacactctcc cccgccgcgc aacagatgct
     5461 cctgaattac agttatccgg gcaatttccg cgaactcgaa aacatcctcg aacgcgccgt
     5521 cgccctgtgc gtcggatata cagtgcaaat cgacgacctg caaatccaag atgtgcacca
     5581 caaacccgtc cggacggaaa ccgccgtccc cgttgccgat accttgccgt ctgaaacagc
     5641 cgccgcaccg tcccgccttc tcccgttcga ccccgatacc atgcagatac aggactatct
     5701 cgacaaaatc gaacgcgaca tcatcggaca agtcctcaaa caaaccgaag gcaaccgcac
     5761 gcaagccgcc aaacgcttgg gcatcagctt ccgctctatg cgctaccgta tggaacgcct
     5821 caacatagac tgacaaacgg catccgcacc atctccgccc acccgaaaaa atgccgtctg
     5881 aaacggcacg gggaagcggg ttcgccccac gaccgaacgg acacaataca ccatgaccga
     5941 catcctcatt gacgataccg ccaccgaagc cgtccgcacc ctgatacggg cattccccct
     6001 cgtgcccgtt tcccaaccgc ccgaacaagg cagttacctc cttgctgaac acgataccgt
     6061 cagcctcagg cttgtcgggg aaaaaagcaa cgtcatcgtc gattttacct ccggcgcggc
     6121 acaataccgg cgcacaaaag gcggaggaga actcatcgcc aaagccgtca accacaccgc
     6181 gcaccccacc gtatgggatg caaccgcagg attggggcgc gacagcttcg tcctcgcctc
     6241 gctcggactg accgttaccg ccttcgagca acatcccgcc gtcgcctgcc tgctttcaga
     6301 cggcatccgc cgcgccctcc tcaatcccga aacgcaagac accgccgcgc gcatcaacct
     6361 ccatttcggc aatgccgccg aacaaatgcc tgcccttgtt aaaacacaag gcaaacccga
     6421 catcgtttat ctcgacccga tgtatcccga acgccgcaaa agtgccgccg tcaaaaaaga
     6481 aatggcttat ttccaccggc ttgtcggcga agcgcaagat gaggtcatcc tccttcatac
     6541 cgcacgccaa acggcaaaaa aacgcgtcgt cgtcaaacgc ccgcgcctcg gcgaacacct
     6601 tgccggacaa gcccccgcct accaatacac cggcaaaagc acccgcttcg acgtttacct
     6661 gccctacgga gcggacaagg gataacgccc ataaaacaag acaccgaaaa atttgccgtt
     6721 cttatgcaaa cgagaaaccg gtttttgcgt ttcgactgtt ttggataagt catcacacct
     6781 taaagtttgt cattcccacg gaagtgggaa tccgattcgt tcagttttag ttgttttcaa
     6841 gtttcaggca acttccaa
//
LOCUS       DAXDXW010000096         6814 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXDXW010000096 DAXDXW010000000
VERSION     DAXDXW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.097"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            89..493
                     /locus_tag="ACFB64_001920"
     CDS             89..493
                     /locus_tag="ACFB64_001920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC1210217.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWHG
                     HIAEITDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DATFPAPIWANPVRKHGMTLPQNWTEQLKKLL"
     gene            533..1714
                     /locus_tag="ACFB64_001921"
     CDS             533..1714
                     /locus_tag="ACFB64_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC1210218.1"
                     /translation="MTEPTSRRRFLKTCTAAGAGLLQACGTSATSVPPLPSSHSVVKA
                     RTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRFQ
                     RFAGTDAQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGTL
                     FFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGTTQNRLTVDV
                     PYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNTL
                     YLSGILGKQRAIVFGDFRMEKIRDLYDSSYDFSAVAKHISRTAKIPVLTGFPFGHIAD
                     KITFPLGAHTRIRMNGNGGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADIS
                     E"
     gene            complement(1817..4072)
                     /locus_tag="ACFB64_001922"
     CDS             complement(1817..4072)
                     /locus_tag="ACFB64_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFC1210219.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     AYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPKLQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNARFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAPDLIFIGGAICVAI
                     QATKIVFGRDK"
     gene            4446..5624
                     /gene="nirK"
                     /locus_tag="ACFB64_001923"
     CDS             4446..5624
                     /gene="nirK"
                     /locus_tag="ACFB64_001923"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HFC1210220.1"
                     /translation="MKRQALAAMIASLFALAACGGEPAAQAPAETPAASAEAASSAAQ
                     ATAETPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGSGAASAPAASAPAASAPAASASEKSVY
                     "
     gene            5802..6557
                     /locus_tag="ACFB64_001924"
     CDS             5802..6557
                     /locus_tag="ACFB64_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFC1210221.1"
                     /translation="MKYVRLFFLGTALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASVGAS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
BASE COUNT         1706 a         1929 c         1712 g         1467 t
ORIGIN      
        1 tggtacaatt aacggcaatg cggacagaca aattaaacta tcagaaattg ccctaccgtt
       61 ttttaaacac tttcaggaat aaggaaaaat gaccgcccaa ccctgcccca tctgcacggc
      121 acaagatgaa gatattttgc tgcaaacccc caagctgcgc gtcatcgccg tccataacga
      181 cagcggttcg ccggcattct gccgcgtcat ttggcacgga cacattgccg aaattaccga
      241 cctttcggca gcggaacgcg gcgaattgat ggaaatggtg tacaaagtcg aagccgctat
      301 gcgccaagtg ttccggccgg caaaaatcaa cctcgccagc ttgggcaatg tcgtgccgca
      361 cctgcattgg catattatcg cccgctttga aaacgatgcg actttccctg cgccgatttg
      421 ggcaaacccc gtccgaaaac acggtatgac cctgccgcaa aattggacgg aacagcttaa
      481 aaagctgctt taaacccgcc gatgccgtct gaaaccgtat gaaagggaaa ttatgaccga
      541 accgacctcc cgccgccgtt ttctgaaaac ctgcaccgcc gccggcgcgg ggctgcttca
      601 ggcttgcggc acatccgcca catccgttcc gccccttccc tcttcccatt ccgttgtgaa
      661 agcccgaacc gtgcctctcc aaacgccacg ccgtcaaagt tcggacggca accttctgcg
      721 cgttgtcgct tcgtcaggat ttgccgaaga cactaaccgc gtcaacacag ccttaacccg
      781 cctttacaat gccggtttta ccgtaaccaa ccaacaggcg ggcagccgcc gtttccaacg
      841 gtttgccggc acggacgcgc aacgcgcggc agatttccaa gaggtcgcct ccggccgcgt
      901 cgccacgcct aaagtgctga tgggcttgcg cggcggttac ggtgcggcgc ggattctgcc
      961 gcatatcgat tttgcctcgc tcggcgcaag gatgcgcgaa cacggcacgc tgtttttcgg
     1021 attcagcgac gtatgcgccg tccaactggc attgttggca aaaggcaata tgatgagttt
     1081 tgccggcccg atggcttata gcgattttgg caaacctgcc cccggtgcgt ttacgatgga
     1141 tgcctttatc aaggggacaa cccaaaaccg cctgaccgtt gatgttcctt atatccaacg
     1201 cgccgatgtc gaaaccgaag gcacgttgtg gggcggcaac ttaagcgtcc tcgcctcgct
     1261 cgccggtacg ccttatatgc ccgacatcga cggcggcatt ttgttcctcg aagatgtcgg
     1321 cgaacagccc taccgcatcg aacgtatgct caatacgctg tatctttcgg gtattttggg
     1381 caaacagcgc gccatcgtgt tcggcgattt ccgtatggaa aaaattcgag atctctatga
     1441 ttcgtcttat gatttttccg ccgttgccaa gcatatttcg cgcacggcga aaatccccgt
     1501 gctgacgggc ttcccgttcg gacacattgc cgacaaaatc actttccctc tgggcgcgca
     1561 cacccgaatc cgtatgaacg gaaacggcgg ctattcggtc gcgtttgaag gctaccccac
     1621 actcgatgcg tccgccctga ctttggatac cctgctccca ccgccggatt tgcctatctt
     1681 ccccgaaagc ggtgttgccg atatttcgga ataaacccgc aaacggacaa atgccgtctg
     1741 aagccttcag acggcatttt cctgtaaaaa aaaccgcacc tgatggcagg tgcggtttcc
     1801 attttcaggc taaaacttat ttgtcgcggc cgaatacgat tttagtggct tggatggcaa
     1861 cgcagattgc accgccgata aagattaagt cgggggcagt gcgtacccag cgcagggtgt
     1921 cgaggatttc catttgcagg aattcttcgc tgcgggcata ccacaaaccg tgcgtgatag
     1981 aggcgtatgc ctgaatcacg ccgacaggca gcaggctgat ggcaatcata ccgaccaagc
     2041 cgccgttgag cagccagaag ccccaagtca tcagtttgtc gtcaaaccgc gcgttcggtt
     2101 tcaagtagcg cgcaaccaac aatacgaagc ccaatgccaa gaaaccgtac acaccgaaca
     2161 aggcggcgtg cgcgtgaacg gcggaagtgt tcaaaccttg gatatagaac agggaaatcg
     2221 gcggattgat cagaaagccg aatacgccgg caccgatcat attccaaaaa gcgactgcca
     2281 cgaagcacat tagcggccaa cgcaggcgtt tcgcccagtc ggacaggtgt tggtaagacc
     2341 agtgctcgta tgcctcgcgg cccagcaaca ccagcggcac gacttccaaa gcggagaagc
     2401 aggcgccgat tgccatagag gcggaggtag agccggagaa gtacaggtgg tgcagcgtgc
     2461 ccggaacgcc gcccaacata aagatggcgg cagcggccaa agtggaggca gtggcggtac
     2521 tgcggcggac aaagcccata ttgtagaaga caaaggcaaa ggcggcagtg gcaaatactt
     2581 caaagaagcc ttccacccac aggtggacca cccaccaacg ccagtattcc attacggcaa
     2641 tcggggattt ttcgccatag aacaggcccg gtgcgtagaa cacgcccacg ccgaccatag
     2701 aagcgacaaa gattgccagc aggtttttgt ccacgccttt ttctttgaag gcggaaaccg
     2761 tgcagcgcaa catcaggaac agccacaaca gcagtccgac catcaaaagg agttgccaga
     2821 aacgtcccaa atcgaggtat tcgtaacctt ggtgtccgaa ccagaagtta aattcggggg
     2881 gaaggatgtg cgtcaacgcg aagaagttgc ccgcgtaaga accgccgacc acgatgaaga
     2941 gggcgatata gaggaagttc acgcctgcac gttggaactt gggatctttg ccgccgttga
     3001 caatcggcgc gaggaacaaa cctgccgtca aaaagccggt tgcaatccag aagatggcgg
     3061 attggatgtg ccaagtacgg gtcagggcgt aggggaacca gtcggacatt tcaaagccca
     3121 acgcctcgtc aatgccgtag aaaccctgac cttcgacggt gtagtgcgcg gtcaggccgc
     3181 ccagcaatac ttgtaccaca aacagggcga ccgtcaggaa gacgtatttg cccaatgctt
     3241 tttgcgaagg ggtcagttgg attttggaaa tcgggtcttc agacggcact tccacttcct
     3301 cgtgtttggt caggaaggaa taaccccaca tcaacaaacc gatgcccatc aagagcagta
     3361 cgacactggt aaacgaccac atgtagtttt cagtagtcgg tacattgttg atcaaaggct
     3421 cgtgcggcca gttgttggtg taggtaaaga cctcgccggg acggttggtc gaagcagacc
     3481 aagaagtcca gaagaagaag tcgaacagtt tttcacgcgc ttcttggctt ggcaatgtgt
     3541 tgtttttcat tgcaaagtgt tcgcgggtgg tttgcaactt gggatcatcg ccgtaaacgc
     3601 cgtgatagta aggcaggatg ctttcgatgg ctttcacgcg cgtatcgctg atgacgacgc
     3661 tgccgtcttc tttaatacgg ctttgattgc ggtattcatc ggccaagcgg gttttcagaa
     3721 cggcttgttc ttcaggggaa acttcatcga attttttgcc gtaagcctgt tgcgcggtca
     3781 aatccaacca ggcggacaac tcacgatgca gccagtcggc cgtccaatcc ggagcctgat
     3841 atgcgccgtg tcccagaatc gaaccgactt ccatgccgcc ggtagtctgc cacgcagact
     3901 gacctgccaa aatatcgtct ttcgtcatca gcactttgcc ggatgcggaa acgacctgtt
     3961 cggggtaagg cggggctttc ttataaacct cgctgcccat atagccaaga atggtaaagc
     4021 ataccgccag aacggcaaac agcaagtacc acagcttctt gtactgtccc attttgagag
     4081 ctccttttaa taaatcagtt gtttaaaatt cacaaaatat gaatgttaaa gattgtagca
     4141 tggtttaccg cgcaaataaa catttgttca aacaaactca catatagaac aaatacatat
     4201 atgataataa ctatcattat tctttactcg gaaattgccc tgcctttgcc tgctctgccg
     4261 gagcccctag caaatcagcc tattcattgt aatttttagt agttataaag tattagaagc
     4321 gtcattttaa gttcatattt tatgaattat ttgacttaaa ttaaaatgcc cgcaatggga
     4381 caaccgcata atcacaccaa gttcttaact aatcccccct acttttctta caaaaggaaa
     4441 acattatgaa acgccaagca ttagccgcaa tgattgcttc cttattcgca ttggccgcct
     4501 gcggtggcga acctgccgcg caagcccctg ccgaaacccc tgccgcttcc gcagaagccg
     4561 caagttccgc cgcacaagct accgccgaaa cgcctgcagg cgaactgccc gtcatcgatg
     4621 cggtgaccac ccacgctccc gaagtacctc ccgcaatcga ccgcgactat cctgccaaag
     4681 tacgcgtaaa aatggaaacc gtcgaaaaaa ccatgaaaat ggacgacggg gtggaatacc
     4741 gctactggac atttgacggc gacgttccgg gccgtatgat ccgcgtacgc gaaggcgata
     4801 cggttgaagt cgaattctcc aacaatcctt cttctaccgt tccgcacaac gtcgacttcc
     4861 acgccgcaac cggtcagggc ggcggtgcag ccgcgacctt taccgccccg ggccgcactt
     4921 ccacattcag cttcaaagcc ctgcaaccgg gcctgtacat ctaccactgc gccgtcgcgc
     4981 cggtcggtat gcacatcgcc aacggtatgt acggtctgat tttggtcgag cctaaagaag
     5041 gcctgccgaa agtggataaa gagttctaca tcgtccaagg cgacttctac accaaaggca
     5101 aaaaaggcgc gcaaggcctg caaccgttcg atatggacaa agccgttgcc gaacagcctg
     5161 aatacgtcgt attcaacggc cacgtaggcg ctatcgccgg cgataacgcc ctgaaagcca
     5221 aagcaggcga aaccgtgcgt atgtacgtcg gtaacggcgg cccgaacttg gtgtcttcct
     5281 tccacgtcat cggcgaaatc ttcgacaaag tttatgttga aggcggcaaa ctgattaacg
     5341 aaaacgtaca aagcaccatc gtgcctgccg gcggttctgc catcgtcgaa ttcaaagtcg
     5401 acatcccggg cagctacact ttggtcgacc actccatctt ccgcgcattc aacaaaggcg
     5461 cgttggggca attgaaagta gagggtgcgg aaaaccctga aatcatgact caaaaattga
     5521 gtgataccgc ttacgccggc agcggcgcgg cttctgcccc tgctgcttcc gcaccggctg
     5581 cttctgcccc ggcagcctct gcatccgaaa aaagcgttta ttaaatcgga tacccgtcat
     5641 tagcgggaca aaccactgcc gccgtacttc attacgcacg gcagtggttt tttaacaacc
     5701 aatctttcct ttcggaagat tgatttttca gacggcattc gcggcttttt gtttaaaatg
     5761 ccgtctgaaa aatcaatgct aaccgcctgt caggaggctt tatgaagtac gtccggttat
     5821 ttttcctcgg cacggcactc gccggcactc aagcggcggc tgccgaaatg gttcaaatcg
     5881 aaggcggcag ctaccgcccg ctttatctga aaaaagatac cggcctgatt aaagtcaaac
     5941 cgttcaaact ggataaatat cccgttacca atgccgagtt tgccgaattt gtcaacagcc
     6001 acccccaatg gcaaaaaggc aggatcggtt ccaaacaggc agaacccgct tacctgaagc
     6061 attggatgaa aaacggcagc cgcagctatg cgccgaaggc gggcgaatta aaacaaccgg
     6121 taaccaatgt ttcctggttt gccgccaacg cctattgcgc cgcacaaggc aaacgcctgc
     6181 cgaccatcga cgaatgggaa tttgccggac ttgcttccgc cacgcagaaa aacggctcaa
     6241 acgaacccgg ctacaaccgc actattctcg attggtatgc cgacggcgga cggaaaggcc
     6301 tgcacgatgt cggcaaaggc cgcccgaact actggggtgt ttacgatatg cacgggctga
     6361 tttgggaatg gacggaagat ttcaacagca gcctgctttc ttccggcaat gccaacgcgc
     6421 aaatgttttg cagcggcgca tctgtcgggg cgagcgactc gtccaactat gccgccttcc
     6481 tccgctacgg catccgcacc agcctgcaat ccaaatatgt cttgcacaac ttgggcttcc
     6541 gctgcgcaag ccgataaccc cttcaattat agtggattaa caaaaaccag tacggcgttg
     6601 cctcgccttg ccgtactggt ttttgttaat ccgctatatt ccgccatctc taagatttac
     6661 agcgatacac gggtaattta aggaatgccc gaaccgtcat tcccgcaact tttcgtcatt
     6721 cccgcgaaag cgggaatcca gaatcccgga ctttcagata atctttgaat attgccgttg
     6781 ttccaaggtc cggactcccg cccgcgcggg aatg
//
LOCUS       DAXDXW010000097         6594 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXDXW010000097 DAXDXW010000000
VERSION     DAXDXW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.098"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..262)
                     /locus_tag="ACFB64_001925"
     CDS             complement(<1..262)
                     /locus_tag="ACFB64_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210222.1"
                     /translation="MPSLQRRSRGEKSSCIKQLNGGNYPPFLICCQLFFENLPDRFKF
                     CKKLARWLSGNKFPQKPVNPIIQKIICFHRVSFSVETLPFRAV"
     gene            complement(571..1035)
                     /locus_tag="ACFB64_001926"
     CDS             complement(571..1035)
                     /locus_tag="ACFB64_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC1210223.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSSTPVTERVCRL
                     EREHYISGYHAHLNPHLLGKPPLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            1257..2315
                     /gene="alr"
                     /locus_tag="ACFB64_001927"
     CDS             1257..2315
                     /gene="alr"
                     /locus_tag="ACFB64_001927"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC1210224.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTKGLEGEESLANSAAILNIPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRISMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(2857..4212)
                     /locus_tag="ACFB64_001928"
     CDS             complement(2857..4212)
                     /locus_tag="ACFB64_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC1210225.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKHLSAKYGVPIVNQRISVTPIAQIAAATKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSVPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDAVSLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPAKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(4227..4499)
                     /locus_tag="ACFB64_001929"
     CDS             complement(4227..4499)
                     /locus_tag="ACFB64_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC1210226.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEILDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(4624..5361)
                     /locus_tag="ACFB64_001930"
     CDS             complement(4624..5361)
                     /locus_tag="ACFB64_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC1210227.1"
                     /translation="MKKLSRIVFSIVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(5499..6398)
                     /gene="prmB"
                     /locus_tag="ACFB64_001931"
     CDS             complement(5499..6398)
                     /gene="prmB"
                     /locus_tag="ACFB64_001931"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC1210228.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAVYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIVPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINIEDYGLEERIQLIHTDLFEGLEGTYDLIVSNPPYVDAESVGALPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
BASE COUNT         1756 a         1640 c         1470 g         1728 t
ORIGIN      
        1 ctaccgccct aaagggcagg gtttcaaccg aaaaggaaac acgatgaaag catatgattt
       61 tttgaataat cggattgacg ggtttttgag ggaatttgtt accggatagc catcgggcaa
      121 gttttttgca aaatttgaat cggtcgggta aattttcaaa aaataattga cagcagataa
      181 gaaacggcgg ataattaccg ccgttgagtt gcttgatgca gcttgatttt tctcctctac
      241 ttctcctttg tagacttggc acacattcaa ccggatgtgt gcatttttta tctccgcttt
      301 ttttgaaatt taattacttt tattgtttga aattctattc tttaagaaat ttaacaagaa
      361 aaacgcttgc tttttgaccg gaaaagctgc ataattcgtt ccgttagctg gttcgagtag
      421 tcagttaata gtttctcctc tatttctcct ttgtagactt ggcacacatt ctattgaatg
      481 tgtgcatttt ttatctgaag caacaagcct ctgtgcgtga tgttgttatg tttcatttag
      541 atgtcaaacc gcatacccgg tctgaaatat tcaatccaaa tccaaaaccg gattttcttt
      601 gacttcctcc atcacaacat aactcctact ctccgaagcg gcaggcagtt gcaataggat
      661 attgccgagc atatcccgat aggcagacat atcgggcaaa cgtactttaa tcaggtagtc
      721 gtattcgccc gacaccagat ggcactccat aatttgcgga attttcagca cttctttttt
      781 gaaatcttcg aaaatattgc ccgatttgga ttgcagcttc agctcgacaa aaaccaatgg
      841 cggttttccc aacagatggg gattgagatg ggcgtgatag ccggaaatat aatgttcccg
      901 ctccaaacgg cataccctct ctgtaacggg cgtgctggac aaccctacct tctcggcaag
      961 ctccgtcatc gggatacggg cattttgctg aagaattttg aggatgcgga aatcgatttt
     1021 atctagttct ttcatttgga ttaccttgta tttattattg attttaacaa atagagtata
     1081 aatttgggaa agcaattata tcaggaaaag caaaccgcct tcctacctga aaactgctgc
     1141 tctggcttga agacacaagg ttctttaata ttttaaaagc cttgccgttg gattataatt
     1201 ccccaattga tttcttaatt ttgctaataa acacttgttt ggtaaggaat gaatttatgc
     1261 ggcctttgaa cgtgcagatc aggttgggca accttaggca caattatcgg attttgaagg
     1321 aaatgcacgg aggcaagctg ttggcggtag tgaaggccga cgcatacgga cacggtgcgg
     1381 tcagatgtgc tttcgcgctg gcagacttgg cagacggctt tgccgtggcg acaatcgacg
     1441 agggaatcag gctgcgggag agcggcatta cccatccgat tgtcctttta gaaggcgtat
     1501 ttgaagcatc agagtacgaa gcggtcgaac aatactcgct ttggccggca gtcggaaacc
     1561 aatggcagct tgaggctttg ctttcccgcc attggaaaaa acctgtcaag gtctggttga
     1621 aaatggattc gggaatgcac cgtaccggat ttttccctca tgattacgct tcggcatatg
     1681 cggcattgaa acagtcggaa tatgtggaca gtattgtcaa gttctcgcat ttctcctgtg
     1741 cggacgaacc tgaaagcggt atgacggaaa tacagatgga agcattcgat ttgggtacga
     1801 aagggctgga aggcgaagaa agccttgcca attcggcagc tattttgaat atccccgaag
     1861 cacgcaggga ttgggggcgc gcgggcttgg cgttatacgg catttccccg ttcggaggca
     1921 gcgatgacag gctgaaaccc gtgatgaggc tttcaacccg tattttcggc gaacgcgttt
     1981 tacagccgca ctcccctatc ggttatggcg caacatttta taccagtaaa tctacgcgcg
     2041 tcggtctgat tgcctgcggt tatgcggacg gttatccgcg ccgcgctcca agcaattccc
     2101 ccgtcgccgt cgacggtaaa ttgacccggg tcatcggcag gatctctatg gatatgatga
     2161 ctatcgagct ggacgcttcg caagaaggtt tgggacacga ggtcgaactg tggggcgata
     2221 cggtcaatat caataccgtt gccgaagcgg ccggaaccat cccttacgaa ttgatgtgca
     2281 atatcaaacg tgcaaaattc acttatatcg agtaatcgag tccaaacgaa aatcccgtct
     2341 gaagcttttc agacggcata aatgcgtttt gacaaaatca gtaaaagaca ttgccaaata
     2401 gaaaatactg ataatgccca caatctgaaa ccccatcctt cccgcaaaaa cggaaaatca
     2461 aaatcagaaa cctaaaattt cgtcattccc gcgcaggcgg gaatccggtt ttttgagttt
     2521 ccgtcatttc tgataaattc tcgtcgtttt tcatttctag attcccgcat gcgcgggaat
     2581 gacaaaatta aagtttcaga atttatttga acaacaaact agattcccgc tttcgtgggg
     2641 atgacggcgg ataggttttg ttgtttcgga taaattcctg ttgttttttg gtttccagat
     2701 tcccaccttt aaaagaatga cgtaagaaag cagaaataag aacacgggga ttttttaaat
     2761 ttgcaatatt cttttctaaa gacattttac ccaatgccca taaacaaaaa tgccgcctga
     2821 agcctttcag acggcatttt cccatcaaaa ccgcaatcag tttttcatcg attgaaccgg
     2881 tgcgggaatc ctgccgcccc ggttgacgaa cacttcgcac gaaccttctt ttgccggcat
     2941 tacaggcgcg tagcccaaca gaccgccgaa ctcgacgctg tcgccgacgg ttttgcccgt
     3001 taccggaata atgcgcacgg cggtggtttt gctgttgatc atgccgatgg cggcttcgtc
     3061 ggcgatgatg ccggaaatgg tgtgcgcggg cgtgtcgccg ggaacggcaa tcatgtccaa
     3121 accaacggag cagacggcgg tcatggcttc gagtttgtcc agcgtcaaca cgcctgcctc
     3181 ggcggcggca atcatacctt cgtcttcgga aacggggata aacgcgccgc tcaaaccgcc
     3241 gaccgcgctg gaagccatca tgccgccctt tttcacggca tcgttcagca atgccaaagc
     3301 tgctgttgtg ccgtgcgtac cgcagacgct caagcccatt tcttcaagaa tgcgcgccac
     3361 cgagtcgccg acggcggggg tcggtgccag cgacaaatcg agaatgccga acgggatatt
     3421 cagcattttt gaggcttcgc ggccgatgag ttcgcccacg cgggtgattt tgaaagcggt
     3481 tttcttcacg acttcggcga cctcggtcag gctgaccgcg tccgaatttt ccagcgcggc
     3541 tttgaccacg cctggaccgg atacgccgac attaatcaca gcatccgctt cgcccgagcc
     3601 gtggaacgca cccgccataa acggattgtc ttccaccgcg ttgcagaaca cgacgatttt
     3661 ggcgcagccg aaaccttcgg gcgtgatttc agccgtgcgt ttgatggttt cgcctgccag
     3721 cttgaccgca tccatattga taccggcacg cgtgctgccg atattgatgg agctgcacac
     3781 gatatcggta gttttcatcg cttcgggaac ggaacggatc aacacctcat ccgaaggcga
     3841 catacctttt tgcaccagcg cggaaaagcc gccgataaag gacacgccga tggctttggc
     3901 tgccttgtcc aaagtctgcg ccacgctgac ataactgtcg gctttggtcg ccgccgcgat
     3961 ttgggcaatc ggcgtaacgg aaatgcgctg attcacaatc ggcacgccgt atttggcgga
     4021 aaggtgtttc gccgttgcca ccaagtcttt gccgaccgtg gtgattttgt tgtaaatgtt
     4081 ttggtttaac acgtcgatgt cggtgctgat gcagtcgtgc aaatcaatgc cgatggtaat
     4141 ggtgcggaca tcaaaattct ggtcggcaac cattttgacg gtttctaaaa tttcgccgga
     4201 ttggatactc atcacattcc tccgactcaa atgcggtgca tcgcttggaa gatttcttcg
     4261 ttttgcatac ggatgtcaag cgcgagtttt ttgctctctt ccgcaaacaa atccaaaatc
     4321 tcctgccgcg atttagggca tttcgaggta tccaccaaga taatcatagt aaaaaaatcg
     4381 tccatcagct gttggctgat attgaggatg ttgattcggt tttccgccaa aattttggaa
     4441 acatcgtaca cgatgccgac gcggtcttta ccgatgacgg tgatgactga attgttcaca
     4501 ggcttactcc ttgcagatat ccgttaaaat ccgaaattat accaccgtcg gattttgaag
     4561 aatattgtca acaatatata catacaaaat gccgtctgaa actatttcag acagcatcaa
     4621 gattcagggt tcgattaaat aaccatcctt atcccattgg gttttcctga ccaacttgtc
     4681 atcctgataa acagcctcgc tctttttaga accatcttca taccactcca aaaccacccc
     4741 gttgcgttta tggtggcgga tagacagttc cgaaagtaat cggccgcttt catcccaagt
     4801 cagaattttg gcaggctcat cgttgaccat aaccatttcc gtcttgatac ttccatcggc
     4861 ataccattgc ttccatacgc cgttcgcctt attttgctta aactggattt cgctttcctt
     4921 gccgccgtta cggtaataac ggtatcccgt accctcactc aagccatttt tataaggcat
     4981 aaccgcagat tttttaccgt tcggatacca gttgacccat tccccgtccg gcttaccctt
     5041 gctgaagccc cccgccattt ttttctgacc attaaaatgc cacaaaatca acataccgtt
     5101 ttgcagggta ggcacaaaag atttgatttg cgttgaagca acgatataag gttcggaata
     5161 tttcttcatc gacggataat aaaaatcctg cgcgtgtgcg atacccgccg ccacactata
     5221 ttgcctgata taagcggcag aagacatcgt cgccgtcagt ttcccgttct gattaaaata
     5281 aacagaatag gtctgcgccg gcaaagcggc cgaaaaaccc aacagtacga ttgaaaatac
     5341 aatccgagat aatttcttca ttatgatagc gatataaaaa acaattaact tataaacaat
     5401 atggtaagga tttctcaacc aagcgtcaaa cccgaaacaa tgtatcgtaa aaatgccgtc
     5461 tgaaaacaaa tcgtcttcag acggcatttc cctccaactc actcttcgcc caataactgc
     5521 tcgcgcgtca agaggaaaac aaaaccgtcg cccccgctgg tttccaacca agtaaaaggc
     5581 aactccggat acgctgcttc caatacatcc ctgttatgcc cgatttccac cagcaataca
     5641 cctttaggat tcagaaactt tgccgcattc agaagaatct gcctggtggc atccaacccg
     5701 tccgccccgc tgcccaacgc caattccggt tcgtgcaaat attcctcagg caaagcccca
     5761 accgactctg catccacata aggcggattg gaaacaatca aatcataagt gccttccagt
     5821 ccctcaaaca aatcggtatg aataagctga atacgctctt ccagaccata atcttcaata
     5881 ttgatccctg caacttccaa agcatccaaa ctcacatcaa ccgcatcaat ttgggcatca
     5941 ggataatggt gcgccatctg aatggcaagg caaccgcttc cggtgcaaag atccaaagca
     6001 ttatgcacca gctcatcgta ttctatccaa ggtcgaagtc cgtcacccag caattcataa
     6061 ataaaagagc gcggtacaat cacgcgttca tctacataaa aatcaaactc cccctgccag
     6121 gcctggtgtg tcaaataagc ggcaggaatg tgttcgacag cacgacgctc aataaccgcc
     6181 agcacttcct ctttttcagc ttccaagagt tttgcatcaa gatatggggc aagcatatcc
     6241 aaaggcaaat tcaaagtatg cagaatcaaa taaaccgctt catcatgcgc attatctgtt
     6301 ccatgaccaa aaaagagccc tgcctcatta aaacggctga ctgcaaaacg taaaacatca
     6361 cggatagtcg tcaattcttg tgcggcctga ttaaacataa tatgaaccat tctgcgtata
     6421 gatgctttta attataacag aaacaacaag caaacctttt catatcgcca aataaccact
     6481 caatctaccc atacaactac ataaatgccg cgcgaagacc attgcccgaa cggaaacggc
     6541 gatggcggac ggtatgggca acctgatagg ctgggaagaa acggggcttg ttga
//
LOCUS       DAXDXW010000098         6556 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXDXW010000098 DAXDXW010000000
VERSION     DAXDXW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.099"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(61..258)
                     /locus_tag="ACFB64_001932"
     CDS             complement(61..258)
                     /locus_tag="ACFB64_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210229.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAV
                     NAGLEEIGNQCEFVVFGFLKD"
     gene            complement(263..553)
                     /locus_tag="ACFB64_001933"
     CDS             complement(263..553)
                     /locus_tag="ACFB64_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210230.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            complement(598..1928)
                     /locus_tag="ACFB64_001934"
                     /pseudo
     CDS             complement(598..1928)
                     /locus_tag="ACFB64_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            complement(2067..2489)
                     /locus_tag="ACFB64_001935"
     CDS             complement(2067..2489)
                     /locus_tag="ACFB64_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC1210231.1"
                     /translation="MTDIFTPSKRSFVMSKIHSKETKPEVLVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWHGHSCNKGHIPKSNMDFWLEKITKNRERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVEEIKDAV"
     gene            complement(2495..3091)
                     /locus_tag="ACFB64_001936"
     CDS             complement(2495..3091)
                     /locus_tag="ACFB64_001936"
                     /inference="COORDINATES: protein motif:HMM:TIGR02391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HFC1210232.1"
                     /translation="MKGESGVDIENWKNKLPEKEREPVEVILNRLEDSELTNKEQAEV
                     ISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTGL
                     HSIDGRELIEKAFGSKKSMLLLTNNKTQAEQNLEDGLEQLACGTWTGFRNPVQHELRA
                     NLSPSIFNDKDALDLISLVSYLLRKVEQTKKRAKPTSP"
     gene            complement(3281..4138)
                     /locus_tag="ACFB64_001937"
     CDS             complement(3281..4138)
                     /locus_tag="ACFB64_001937"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC1210233.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFRLGNKIEISTIQGNERVTFTLDYAEIKKSERIYQPEFQKESVKPNTENGTTIT
                     LTELTKKQGYPLDNYVGHLSRLFDFPAQDFKIKVSLNGSEPRIIDGNLKYNLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHF"
     gene            complement(4152..4586)
                     /locus_tag="ACFB64_001938"
     CDS             complement(4152..4586)
                     /locus_tag="ACFB64_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1210234.1"
                     /translation="MAGHQDETDFMHSCAGLSDINLKRLALIPKNGGNRLAFAHIPEL
                     QLECFIGKDNSFKDTFGRLWWDKPAPTITTKFFSISNGRFAHPEEDRALSLREGATLQ
                     SFPRNYVFKAGSRDKIARLIGNAVPPMYAEKIGRAIVDNIEC"
     gene            complement(4520..5128)
                     /locus_tag="ACFB64_001939"
     CDS             complement(4520..5128)
                     /locus_tag="ACFB64_001939"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC1210235.1"
                     /translation="MQVLAGIDYELSCKETYEANINAKFIQADIFELQPETLEKELGL
                     KKNDDDLILIGCSPCQYWSVIQTDKRKSEKSKSLLLEFQRFVEYFNPGYVVVENVPGI
                     LSRMKESGLDNFIKLLEEKGFTVHFGIHNTADYGIPQSRKRFTLIANRITKKSWNQSS
                     IRANGLRYAMFWEWKTAFPKLWQDIKTRRILCIVVRDYPISI"
     gene            5497..5643
                     /locus_tag="ACFB64_001940"
     CDS             5497..5643
                     /locus_tag="ACFB64_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210236.1"
                     /translation="MSRIYLPLLFPPHIVERGLLYFQQDKVRDVQKISLGRYRAKVFD
                     SENY"
     gene            complement(6020..6469)
                     /locus_tag="ACFB64_001941"
     CDS             complement(6020..6469)
                     /locus_tag="ACFB64_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC1210237.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         1774 a         1512 c         1151 g         2119 t
ORIGIN      
        1 aaaaagtgtt tggcggtaat ggatggagag tttttcagac gaccgccgaa catccgaaaa
       61 tcagtctttc aaaaatccga atacgacaaa ttcgcattgg ttgccgattt cttccaaacc
      121 tgcgttaacc gcttcttcga agtcgtagaa ataatcggca ttggtaatta atttggtatg
      181 tccgatgtcg cccgtttcag gagagagata cagaaagtcc cctgttgata cggactggac
      241 aacatagact ttctgcattc aatcagcctt tcttaacgag ttgaaaaccg atgactttca
      301 gtttttgagt tttgcccgta gtgacgattt ccacgttcag gtttgcttcg atcggaaatt
      361 gggcgtttcg gaactgctcg aaattggcag agccgccgaa atcgtattca gtagtagagc
      421 tgcccaatgc gttgccttgg gagctgtcta agggtgtggc gacaatcagg cggcaatagt
      481 cgaagctctt gccttcgatt tgtccgttga attttttaac gccgacgatg tggccttgaa
      541 gttggatgtt cattttttgg tttccttgtg tgattaaacg tctttcgggc agacacttta
      601 agcccatgaa atcggtaaat ttgcgtattt gtcatacatc ttctctaaat aacgctgctc
      661 ttcccctgtt ttttgacgct gaatatatcg atccatccac atgccgtatt ggtcaaattt
      721 tagaacggac ggaatttcat gcagtggcgt tagctttgcc tcgctgcaat cgtaattctc
      781 gaagctcagt tttttaggca aaaaatcatg tttcggctca agcacttcaa acaattcttg
      841 tttggatttg tccggaaaca ctgctttcga tgcattgatt cctcgtccga tttgcttttt
      901 cagtcgttct atataggtat cgaaatcaat catgatcttg cttttgaccg cttcaatacg
      961 gtttgcactg cctgtaaacc gttcgcaaat cgggtatgct ccgccgaaat actcgcccgg
     1021 tgtctgtaag acttcaaagg gaattacaat gtctttcgct ttgaattcga tttcaaagcg
     1081 cgtccatgtg cttgttttat cgccaagctg cttgcctttt tcatagattc gtacatattt
     1141 tgaagactca cgcgaaccta tgccgtaggt tttgccgctc gtcattttgg tttcgtcgtc
     1201 ttcttcctcc caatcagaac caagacactc gcccttcggt ttgacgtgat ggcacgtaaa
     1261 caaaccttta ttgcggtctt ctcttgcctg attcgggctg tactctccgt tgaaaaagtc
     1321 ttttgcaatg tcaacgcgcg ttatttttgg acgaatcgca tttgtcagaa aatcaaaaag
     1381 ccttgattcc catccaatat ttgcagcatt gcagcctacc gccgtcaatt caaccaacat
     1441 cgtttcacgt tggccgccgt aatggacgcg cccgtattgg gcattttccg ttcccatcag
     1501 ccagcagcct tcatagaaac gcccgcccga atgcttggct ttttcaatga ttccgaatcc
     1561 gaaaatttcc tccatcttgg cggaagccgc gcgtatgaaa tcgtcatcat cgaacaaatc
     1621 aaacgaaagt ccataaacat ggaaaaacgt gtcttcatga attgaaaatg tgatttggtc
     1681 aatgaaagcc gaatctgata caccgcgcct aagaggaacg cctaacaggt ttcctttacc
     1741 gtccgttatg tacgtttcgt aacattcgaa gacttcctga accctgcccg ccgtttcggt
     1801 ttctgtgccc ccccctgtta gataaggggg gggggaagat tcgaagcggt tgccggcttc
     1861 ctgccgtccg ccggcgcctc cgtcatcacg ccggcaacct cctttgtcat cccttgctta
     1921 tcttccattt gcgaatcctc aaaaacgggc aaaaaaaaag ccctgttact tgtagaaagt
     1981 aaagggcgtt aatttttgtt aatcacccct tcttagggac gcaatatata agggtcttaa
     2041 ttaaaggaag atgccgtctg attttttcag acggcatctt ttatttcctc gaccaaccga
     2101 tttaatcgct ccctacaaat agctttattt tttaattcgc actcccaaac gacaatcact
     2161 ttgaaaccaa tcttttccag ctcggtttca tttttaatat cacgttcgcg attttttgtg
     2221 attttttcca accaaaaatc catattgctt ttcgggatat gtcctttatt acaagaatgc
     2281 ccgtgccaaa aacagccgtg tataaacacg actgttttat atttaggcaa aacgatatcc
     2341 ggtttgcctg catagcgctt atcgtttttc cgataacgga aaccttggga aaaaagaaat
     2401 ttccttacca atacttccgg tttggtttct ttgctatgga tttttgacat cacgaaggaa
     2461 cgtttggatg gagtgaaaat atcggtcata aaatttaagg cgatgtaggt ttagctcgtt
     2521 tttttgtttg ttccactttt ctcaataaat atgaaactaa gctgattaaa tccaaagcat
     2581 ccttgtcatt aaaaatacta ggagataaat ttgctcttaa ctcatgttgc acaggatttc
     2641 taaatccagt ccatgttccg catgcaagct gttcaagacc atcttctaaa ttttgttccg
     2701 cctgcgtttt gttattcgtt aatagaagca tagatttttt actcccgaag gctttttcaa
     2761 tcaattctct tccgtctatg ctatgaagac ctgtctgctt ctgaacctta tcttcgaaaa
     2821 ctttaactgc ctcgattgca gcacttaaat aatccttctt ttcattgtaa aaatcgttac
     2881 aggcagtatg caaatcctga tgcaaatgcc gccaatgata gtaaggatat tctggaataa
     2941 tactatgtaa ggcagagatt acttccgctt gttctttatt tgtaagctcc gaatcttcca
     3001 aacgattcaa aataacttct accggttctc tttccttttc tggcaatttg tttttccaat
     3061 tttcgatatc aactccgctt tcacctttca cttcgtaatc tttctctttt tttcttcttt
     3121 ttctccaatc ttgcccgatg gctcagacga tttccccaag gaaaacccgt aattttgaag
     3181 ttttaggatg cttccaatcc aatgatgtcc tatctgtagc gattaaatct tcaccatcat
     3241 tatctataaa atcaatactt aaccatcctg tcagatagga ttagaaatgg ctggattcgc
     3301 tatcagtgaa aaactcgggc atatttacca ttctgccgtt ggcaaacaag gtaatacctt
     3361 tcatattatt ctttaaaggt ttttccgttg taatgaactt accttgtatt aatccgctgt
     3421 attcatactg ttcgaatttt gaagataacg atgaaatatt ggttgctaga tcctggtatt
     3481 cccattcgaa ttgtggggta acaagattat attttaggtt tccgtcaatg attcttggtt
     3541 ccgagccgtt caagcttact ttgattttaa aatcctgagc cggaaaatca aataaacggg
     3601 aaagatgccc cacataatta tctaacgggt atccttgttt tttcgtcagc tcggttaaag
     3661 ttatagttgt tccgttttcg gtattgggtt taacagactc tttctgaaac tccggttgat
     3721 aaatacgctc acttttttta atctctgcat aatccaaagt aaaagtaacc cgttcgtttc
     3781 cttggatagt agagatttcg attttgttgc caagcctgaa taatgccaat ttaccaagac
     3841 cttttttacc cgttggaatt cttccgcacg gggaggcttg tttttcttcc cttctgttcc
     3901 gaccgattct caaataaaaa tcattgattt catcgaagct cattcctatg ccattatctt
     3961 taataacgat tttatgctcc ggtttatcga ataacctaac ttccacttcc gtagcacatg
     4021 catcgtaggc attcgctatc aactcggcaa tcgcaggaac agtgtgcgaa tacatcttta
     4081 cacccaaatg ttcgattacg gttggctcga aactcatttt caatttttct tcagacatgt
     4141 tttctccttt tctaacattc gatattatca acaattgccc tgcctatttt ttccgcatac
     4201 atcggaggaa cggcattacc aatcaaacgg gctatcttgt ccctactgcc cgctttaaag
     4261 acatagttac gagggaacga ttgcaatgtt gcaccttctc gcagggataa cgccctatct
     4321 tcttcaggat gggcaaaccg cccgttggaa atgctaaaga atttggtcgt tatggtaggt
     4381 gcgggcttat cccaccatag cctaccgaac gtgtctttaa aactattgtc ttttccgata
     4441 aaacattcca gctgtaattc gggaatatgc gcaaaagcca aacggtttcc tccgttttta
     4501 ggtatcaaag ccaatcgttt caaattgata tcggataatc ccgcacaact atgcataaaa
     4561 tccgtctcgt cttgatgtcc tgccataatt ttgggaaagc cgttttccat tcccaaaaca
     4621 tcgcgtaccg taagccgttt gcccgaatac ttgactggtt ccagcttttt ttggttattc
     4681 tgtttgcaat taacgtaaat cttttacggc tttggggaat tccgtaatca gccgtattgt
     4741 gaataccgaa atggacggta aatccttttt cttccaataa ctttatgaaa ttatcaagcc
     4801 cgctttcttt cattctgctc agaattcccg gtacgttctc tacaacaaca tatccgggat
     4861 tgaaatattc tacaaaccgc tgaaactcca aaagcaaact cttggatttt tccgattttc
     4921 ttttgtctgt ttgaataaca ctccaatatt gacaaggact acaacctata agaatcaaat
     4981 catcatcatt cttttttaag ccgagttctt tttccaaggt ttcaggctgc aattcaaaaa
     5041 tatcagcctg tataaatttg gcatttatat tggcttcata tgtttctttg cagctaagtt
     5101 cgtaatctat cccggctaaa acctgaatac cggcggatta cattccatag ctcattccgc
     5161 ctccgccaca gaaaaaatca accgccttta acttactgtt tttcatttct atttaaataa
     5221 ttagactagt gagaactaac tttatataac atataattta ttgcttatca aaaataattt
     5281 gagattttat taaaattcat gcatctaaat aagcagaact taatttcctc caaacgatcg
     5341 gatatcttca aatgcacatt tcacagaaag gtcgtctgaa acccattttc agacgacctt
     5401 tttccacttc gcaaaacaag aattcccacc cctctcccca aacatcgccc aaaccagtaa
     5461 attccccata tttcaacaac tccttggaag caaaccatgt cccgtatcta cctacccctc
     5521 ctatttccgc cccacatcgt cgaacgcggc cttttgtatt ttcagcagga caaggttcgc
     5581 gatgtccaaa aaatttcctt agggcgttat cgggcgaagg tattcgattc ggaaaactat
     5641 taggcatggt tgaagctgga tagtgatttg tacattaagg acggggctgc gattgccctt
     5701 atgtctaaag gtgcaaacat gccttaaatt actaagccta taacgaattg gaaacaaaat
     5761 gccgtctgaa aacatcttca gacggcatta taaaatctgt tcaccttttc agatgagtaa
     5821 tgtacacccc tatacaattt ttgctaccat gcaccataaa tccacggcta aagataattg
     5881 ggtttattct tttatccaga aacaatccag atacggatca cgggtcataa ctataggctt
     5941 aatattacac gattctcatt ccatcaaggc ggaaaaccgc acaaatactg aaacactatc
     6001 gatcgatttg taaacaagcc tacttaagta acttgcagtc cttatcattt cctttaaaat
     6061 aatccagccc gtcactacac gaactggcgg acttcttgca aataaaggtt actagatttt
     6121 cattcatctt aataataaaa ggatttttat ctttatctat ggctaccgcc ttcaacatga
     6181 atttactgtc taaagccccg cgcgcgattc cattcaaacg gatacaaaag ccttctgcct
     6241 ctttaatcgg caaacttggc catttggtag atgtttgttt aaatctccca ttctgcagat
     6301 aaaacttttc cataaaatgt gcattttcta acaaggctgc ccgcactgca tttatctttg
     6361 ctttctcaac ataattgcga tagctcggat aaacaattaa agcaagtaca gacaatatca
     6421 agaccactga tattaattca accagcgtaa acccccgatt atcagtcatt actttacttc
     6481 caataagaac agattattca acatatttct ttgaacagac ttactatccc attcagcagt
     6541 atgcataatt tcccac
//
LOCUS       DAXDXW010000099         6252 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXDXW010000099 DAXDXW010000000
VERSION     DAXDXW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.100"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            230..1018
                     /locus_tag="ACFB64_001942"
     CDS             230..1018
                     /locus_tag="ACFB64_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC1210238.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDTGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            complement(1064..1663)
                     /locus_tag="ACFB64_001943"
     CDS             complement(1064..1663)
                     /locus_tag="ACFB64_001943"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC1210239.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            1791..2408
                     /gene="gmk"
                     /locus_tag="ACFB64_001944"
     CDS             1791..2408
                     /gene="gmk"
                     /locus_tag="ACFB64_001944"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC1210240.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSTEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLKGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLEKAEGDLLHIVNACRLKRSRQLGFIADLLENS"
     gene            2466..2672
                     /gene="rpoZ"
                     /locus_tag="ACFB64_001945"
     CDS             2466..2672
                     /gene="rpoZ"
                     /locus_tag="ACFB64_001945"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC1210241.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            2765..4921
                     /locus_tag="ACFB64_001946"
     CDS             2765..4921
                     /locus_tag="ACFB64_001946"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC1210242.1"
                     /translation="MPAPQPSAPYDPLTAEARTLLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGDPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYRELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGTLHTLYQPKPGRFKDYIAIPKSNGYQSLHTILVGPYGLPIEVQ
                     IRTREMDAVAEGGIVGHWSYKSDSKTVDQAVLHTNQWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARVNNVMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLDQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(4988..6172)
                     /gene="coaBC"
                     /locus_tag="ACFB64_001947"
     CDS             complement(4988..6172)
                     /gene="coaBC"
                     /locus_tag="ACFB64_001947"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC1210243.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTNLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACRENGM
                     GRMPEPAELLDLLPDLWTPKILRDKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEISLIHGQLQTTLPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDTLASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKTTNQIVIIDDDAELSFPETSKDEAAMRIVERLAVYL
                     NK"
BASE COUNT         1563 a         1501 c         1669 g         1519 t
ORIGIN      
        1 tagggcggtg ctttatgtgt cgtcctgtgt gttgaagcat gatttgtatg ctttgtaaaa
       61 atgtttcaga cggcattgct gcaaatgccg tctgaagggt gtttgaagat gggcggatta
      121 tccgcctttt cgtgttcgcg gtcaattttg ccgggaaatt tataaggatt tatcaagtat
      181 ttgccttctt gggatacaat gcggcagttt gagtgcgaga gagaatatga tgaatcccaa
      241 aatcgtgttt ttcgacattg acgatacgct gtaccgcaaa tatacggata ccttgcgccc
      301 ttccgtgaaa acggcggtgg cggctttgcg cggcaaaggt atattgacgg cgttggcaac
      361 ggggcggtct ttggcgacga ttcccgaaaa ggtcagggac atgatggcgg aaacgggtat
      421 ggatgccgtg gtaacgataa acggacagtt tgcgctgctg cacggtaaaa ctgtgcgcga
      481 agtaccgatg gataccggtt tgatgggcag ggtttgtgcg catttggatg gcttgggcat
      541 ggattatgcg tttgtcggcg gagaggggat cgccgtgtcc gcgctgtcgg aatgcgtgtg
      601 ccgcgccttg cagcatatcg ccagcgattt ttttgccgat aaggattatt tttcaagcaa
      661 accggtgtat cagatgctgg tgtttgcgga ggaaaacgaa atgccgcttt ggtcggatat
      721 tgtggaacgg gaaggcttga aaacggtgcg ctggcacgag gaagcggtcg atctgctgcc
      781 tgcgggaatg tcgaaaacag acggcatcag aagcgtggtt gaagcattgg gtttggaaat
      841 ggcagacgtg atggcgttcg gcgacggttt gaacgatgtg gaaatgctgt ctgaagtcgg
      901 gttcggcgtg gcaatgggta acggggaaca ggcagcgaaa gaagcggcga aatatgtttg
      961 ccccggcgtt gatgaagacg gcgtgttgag gggcttgcaa gatttgggtg tgatttgaac
     1021 gtataataac aaccctgccg gtttcagacg gcagggtcgg ttttcagcct ttcatgcagc
     1081 cttcgttttg aagcagggta aataagggcg cgccacttgc gcggatattc ttgccgcctt
     1141 gaaggtcggt aaattccaaa atggcggcgg cttcgacaat ttccccgccg agtttgcgga
     1201 tcagttccag cccggcaagc attgtgccgc ccgtggcaac caaatcatcg accagcagga
     1261 cgcgcgaacc gggtttgacg gcatcggtgt ggatttccac cgcagcttcc ccgtattcga
     1321 gcgcgtagct ttgcgatacg gtttcaaaag gcagcttgcc ttttttgcgg atggggacga
     1381 agccgacgtt gagctggtag gcgagtgccg cgccgataat gaagccgcgc gcgtccaagc
     1441 cggcaacgat gtcgattttc tgatccatat agcggtaaac cagcaaatcg accaaaaggc
     1501 ggaagtattc cgcactttgc aggacgggcg tgatgtcgtg gaataagatg cctttttgcg
     1561 gccagttttc gattttgcgg attttgtcgg caagcgcgcc gacactcata gcttcgggat
     1621 gaaccagcat tgcgtgttcc aagtttgatg tttttaacgc catattgtag ccgtcagcct
     1681 gccgcttgcc aaatcaatca aaacaatagc ggcaaaatat gggcatcggc aaatatgaaa
     1741 tagggtgtcc gttgtgatac aatcggttgg aaaatataca ggtaaaaaat atgtccgctt
     1801 atcgaaaagg caacatcttt atcatttcgg ccgcttccgg cacgggcaaa accacgctgg
     1861 tgtcgcggct gttggcaaac cataacggtt tgcgcgtttc cgtgtcgcat acgacgcgcc
     1921 cgccgcgtga aggcgaagca aacggcgtac attatcactt tgtttccaaa gaagagtttg
     1981 aatcgcttat cgcgcaggag gcttttttgg aatacgcaga cgtattcggc aactattacg
     2041 gcacaagcac ggagggcgtg aatgcgttgg cggcggcagg ctatgacgtg attttggaaa
     2101 tcgacgttca gggcgcggcg caggttcgca acgcgctgcc ggaagctgtc ggtatcttta
     2161 tcctgccgcc ttctttcgac gtgcttgccg cgcgcctcaa agggcgcggg acggacagtc
     2221 gggaagttat ccaaaggagg ctgtcgaagg caaggcatga aatcgaacag tccgtattgt
     2281 tcgactttgt cgtggtcaat gacgacctgg agaaggcgga gggggatttg cttcatattg
     2341 tgaatgcctg ccgtctgaaa aggtcgcggc aactgggatt tattgcagat ttgttggaaa
     2401 attcctaaaa accgcgaaaa tatccggttt cccaatttaa atatttttga aagaaagcaa
     2461 ataatatggc acgtattacc accgaagact gtaccggaaa aatttccaac cattttgacc
     2521 tgacattggt agcggcccgt cgggcccgcc agcttgaaaa tggcaatacg ccgcttgtgg
     2581 acgatgtccg caataacaaa ccgaccgtta ccgccttaag ggaaatcgca gccgggcata
     2641 tcggtacgga actgttgacg cgcaataaat aaattctgcc ggaaacgcac gccggcacac
     2701 tctgccgccg tgcagtccga cggtttgaaa tgaaaaaaca catacatccc gaaagccgtc
     2761 tgaaatgccc gccccccaac cttccgcccc ttacgacccc ctgaccgccg aagcgcgtac
     2821 cctgcttttc cataccgcct cctacctcaa gcccgaggaa caggcggagc ttgaaaaagc
     2881 tgtcgcctat gcgtttcgcg cccacgacgg gcaaacccgc aaaagcgggg atccctacat
     2941 cacgcatccg attgccgttg cgacgcagct cgccctttgg catatggaca tacagggtct
     3001 ttgtgcaggc gtgatgcacg acgtattgga agatacgggc gtgacaaaag gggaaatggc
     3061 ggcggtgttc ggcaatactg ttgccgagat ggtggacggg ctgtccaaac tcgaaaaact
     3121 caaatttgaa gatcatgcgg aacatcaggc ggagagtttc cgcaaactga ttttggcgat
     3181 gaccaaagat gtgcgcgtca tcgttgtcaa acttgccgac cgcctgcaca atatgcggac
     3241 gctcggttcg atgcgcccgg acaaacgccg ccggattgca agggaaaccc ttgaaatcta
     3301 cgcacagatt gccaaccgca taggtctgaa taatgcgtat cgggagcttc aggatttatc
     3361 gttccaaaac ctgcatccca accgctacga gactttaaaa aaagcgatgg acaagagccg
     3421 gaagaacagg caggacgttg tcggcaaagt tttgcgcgcg ttcggccagc ggctggtagg
     3481 cgcgaatata gaggccaaaa tcaaaggcag ggaaaaaaac ctgtacggca tccatcagaa
     3541 aatgatggcg aaaaagctgc gttttgccga ggttatggat atttacggtt tccgcgtcat
     3601 tgtcaacagc attcccgcct gttatgccgc actcggcaca ttgcacaccc tctatcagcc
     3661 caagcccgga cggttcaaag actatatcgc cattccgaaa agcaacggtt atcaaagtct
     3721 gcatacgatt ttggtcggcc cttacggctt gccgattgaa gttcagatac gcacgcggga
     3781 aatggatgcc gtggcggaag gcggtattgt cggccattgg agctataaat cagattccaa
     3841 aacggtcgat caggcagtgc tgcacaccaa tcaatggttg aaaaacattt tagacttgca
     3901 ggcaagcagc gccaatgcca ttgagtttct cgaacacgtc aaagtcgatt tgtttccgaa
     3961 cgaaatctac atccttactc cgaaaggaaa aatcctaact ttgcccaaag gggcaacgcc
     4021 tgtcgatttt gcttatgcgg tgcataccga cattgggcat aaaacagttg ccgcgcgtgt
     4081 caataatgtg atgatgccgc tgcgtacgaa gctcaaaacc ggtgattctg ttgaaattat
     4141 cacatccgaa cacgccaaac ccaatcccgc gtggttgaat ttcgccgtgt ccggcagggc
     4201 gcgcagtgcc atacgccaat atattaaaaa tcttaaccgg cacgatgcgg tcgttttggg
     4261 agagagcctc ttacaaaaag ccttgtccag tttgctgccc aaagatgtcc tgctttcaga
     4321 cggcatcaaa gaaaaatacc ttgccgatct caacgacaaa cagacttcat ttgaggaagt
     4381 gctgtacaac gtcggtatgg ggcataccct gcctgtttat gtcgccatgc atatcgccga
     4441 attggcaggg gagcatttcg gcagcgaggt caggctcagt tccattaaag ttgatggtca
     4501 ggaaagcggg catattcatt ttgcagagtg ctgccaccct gttcccggcg attccatccg
     4561 tttgctgttg gttaagggaa aaggcatgat tatccatagg gatacctgcc cgacgttgtt
     4621 gaagtccgat cccgaacagc agctggatgc agactgggaa aatatgaacg ggcagaacta
     4681 ccgtgtcggg cttcaagtcc aatcggaaga cagccacggc ctgttggcat taatggcgca
     4741 agcgatttcc gattccggtg cggacatcga gtcggtcgaa acaccgtcta aatcccagtc
     4801 gggaacggaa ggttttgtcg aattcaaatt cttattgaaa gtcaagaatt tggatcagtt
     4861 gaatcagatt attcaaaatc tgcacagcat cccatatatc cgcaaagtca tcagaagttg
     4921 aagcaggtaa aacgggttgc ctgttaatca aaaggcaacc cgttttatgg tttatccgtt
     4981 caattgctta tttgttcaaa tatacggcaa gcctttcaac aatccgcatt gccgcttcat
     5041 ctttacttgt ttcggggaaa gacaactccg catcatcatc tatgataaca atttggttgg
     5101 ttgtcttgcc catcgcgatt gaaacatcat tggcaactat caccggtatt tttttcttaa
     5161 tacgtttttc ccgcgcatat gccattacat tctccgtttc agcggcaaaa ccgatgcaga
     5221 acggcgggtt cggcaatgag gcaatagaag ccaaagtatc ggggttctca tccaattcga
     5281 tggataacgg tttggcattt ttatcttttt tgaatttttg cgtactcctg tttttaacct
     5341 tatagtctga gacggcggca acagaaataa aagcatcttg tttttcaatc aaacgatgta
     5401 ctgcgcgatg catatcttcg gcactgacgg cttgaaccgt atcggatatg ccgaaaggca
     5461 gcgtggtttg aagctgtccg tgaatcaggc tgatttccgc accggcggca cggcacgccc
     5521 gcgccaaagc tacgcccatt tttccgctgg aggtatttgt gatgcccctg acagggtcaa
     5581 tggcttcaaa tgtcgcacct gcggtaatca agattttctt gtcccttaaa attttcggtg
     5641 tccacaagtc tggaagcaaa tccagcaatt cggcaggttc cggcatcctt cccataccat
     5701 tttctctgca agcctgttcg cccaagcccg gcatatagac agtaatgccg tctgaaacca
     5761 gttgtgcgat attccgttgg ttggcaggat tcagccacat ttccacattc atcgcgggag
     5821 cgatggcaag agggcatttc cgtgcggctg ccagattggt cagtaggtta tctgccacgc
     5881 cgttacagat ttttgccacg gtattcatac ttgccggcgc aatcagaaaa acatccgcat
     5941 tccgggtcag gttgatatgt tccataccgt ttgaaccgtt gccgccgtgc gtgtcggtca
     6001 gaacgggatt gccgctcaaa gcctgaaaag tcagcggaga aacaaattca gttgccgagc
     6061 ggctcataac caccgtaacc gaatgcccct gttttttcag cagtcgcacc aactcgcaag
     6121 acttatacgc cgcaatactg cccgttacac ctaaaagaat atgtttgccc attttgacaa
     6181 tttctaatta cgaagaaaaa acagcagcca ttctataaca aagtacggat acattagtta
     6241 ccgtttggat ac
//
LOCUS       DAXDXW010000100         6249 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXDXW010000100 DAXDXW010000000
VERSION     DAXDXW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.101"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            70..405
                     /locus_tag="ACFB64_001948"
     CDS             70..405
                     /locus_tag="ACFB64_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210244.1"
                     /translation="MPSEALSGVQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WGSVGKAAKAVGEGLIEADNEHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKGAAY
                     AVLKERGEV"
     gene            598..3288
                     /gene="glnE"
                     /locus_tag="ACFB64_001949"
     CDS             598..3288
                     /gene="glnE"
                     /locus_tag="ACFB64_001949"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC1210245.1"
                     /translation="MSDHRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKALTDEDFQ
                     AFADWDKIRAEESEEELARQLRELRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYQDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNGIIADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKSLVRPFVFRKYLDYGAYEAMRNLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKIAEMGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPISPEQRQLLAESMGFDSYAAFSDGL
                     NVHRNKVNQLFNEILSEPEEQAQSNSEWQWAWQEKPDEEERLGRLKEHGFDAEAVTAR
                     LEQICHGHKYRRLSAHAQPRFDTIVPLFVQAAAEQNNPTDTLMRLLDFLENISLRSAY
                     LAFLNEHPQTLAQLAQIMSQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DGIEACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWADMPKKHRDTPQFAVIGYGKLGGKELGYASDLDLVYLYDDPHPEAGDVYSRLAR
                     RLTNRLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFGKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRLYRRQ
                     QHNTKLRDAAKTEVSDELLSHYGNVRKLWREVFGEEAATA"
     gene            3712..5919
                     /gene="uvrD"
                     /locus_tag="ACFB64_001950"
     CDS             3712..5919
                     /gene="uvrD"
                     /locus_tag="ACFB64_001950"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC1210246.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLGAPDPHTSRMIGCYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRRHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVAAFVRLIEALRNQVGQMHLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIASRFVEEIPPEVLHYLSVKKPAFDSYGNTRQTTVQRDKIIDDFKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
BASE COUNT         1563 a         1913 c         1579 g         1194 t
ORIGIN      
        1 cccggttata ttctcactgg gaacactatt tgaacaacgg cggcgggcag ggttaaaacc
       61 ggcaatccga tgccgtctga agcgttatcc ggcgttcaga cggcattttg ttttccgaca
      121 gtttataaac tgtcgttgtt tcttgacaga aacaacgacc ttatttattt gaaacgattg
      181 gaggacatga ttatgggttt ttggggtagt gtgggaaaag cagcaaaagc agtaggcgag
      241 ggattgattg aagccgacaa tgagcataag gcattgaaaa tggaatatgc ggagaaatca
      301 agtgaggagc tgcatgaaat cgtaaagagt gatggttttt ttaaaaattc cacacgggag
      361 aaaggtgcgg cttatgctgt tttaaaagag cgtggcgagg tttgaacggg aaacggcggc
      421 atttgccgct gttttttatc ggcaggcatc cgtccgaata tcggggcaag gtttcagacg
      481 gcatcgaagg ttgctatgat atagtggctt gactttaaac cggtacggct ccgccttgcc
      541 ctgatttgaa gttcatccgc tatcacattc ccgccatcct ttcaaacgga atccgaaatg
      601 tccgaccacc gcctcgacac cgcccgccgc cattccctgt tcctcgcccg ccagctcgac
      661 aacggcaaac tcaagcccga aattttcctg cccatgctgg acaaggcgtt gacggatgag
      721 gatttccaag cctttgccga ctgggacaaa atccgcgcgg aagaaagcga ggaagaattg
      781 gcgcggcagt tgcgcgagtt gcgccgttat gtggtgtcgc agattatcgt gcgcgatata
      841 aaccgtatca gcgatttgaa cgaagttacc cgcacgatta cgctgtttgc cgattttgcc
      901 gtcaataccg cgctggattt cgcctacgcc tattatcagg acatgtacgg cacgccgatc
      961 gggcgttata ccaaatcgcc gcagcatttg agcgtggtgg cgatgggcaa ggcgggcggc
     1021 tatgagttga acgtgtcttc cgacatcgat ttgattttcg tctatcccga atcgggcgac
     1081 accgacggca ggcgcgaacg ggggaatcag gagtttttca ccaaagtcgg gcagaaactg
     1141 attgcattgt tgaacggcat tattgccgac gggcaggtgt tccgcgtcga tatgcggctg
     1201 cggccggacg gtgattcggg cgcgttggta ttgagcgaaa ccgcgctgga gcaatatctg
     1261 attacgcagg ggcgcgaatg ggaacgctat gcgtggtgca aaggccgcgt agtcacgccg
     1321 tatccgaacg acatcaaatc gctggtgcgc cccttcgtgt tccgcaaata tctggattac
     1381 ggcgcgtatg aggcgatgcg caacctgcac cgccaaatcc gcagcgaagt cagcaaaaaa
     1441 ggcatggcgg acaacatcaa actcggcgcg ggcggcatcc gcgaggttga atttatcgcc
     1501 cagattttcc agatgatacg cggcggccaa atgcgcgcgc tgcaactgaa aggcacgcag
     1561 gaaacgctga agaaaattgc cgagatgggc attatgccgt ctgaaaacgt cgaaaccctg
     1621 cttgccgcct accgcttcct gcgcgatgtc gaacaccgcc tgcaatactg ggacgaccgg
     1681 caaacccaaa ccctgccgat ctcgcccgaa cagcggcaac tgctcgccga aagcatgggt
     1741 ttcgacagtt atgccgcttt ttcagacggc ctcaatgttc atcggaacaa agtcaatcag
     1801 ttgttcaacg aaatcttgag cgaacctgaa gaacaagcgc aaagcaatag cgaatggcaa
     1861 tgggcatggc aggaaaaacc cgacgaagaa gaacggctag gccgtctgaa agaacacggg
     1921 ttcgatgccg aagccgtcac cgcaaggctc gaacaaatct gccacggaca taaataccgc
     1981 cgcctttccg cacacgccca gccgcgcttt gacaccattg tgccgctgtt cgtacaggcg
     2041 gcggcagagc aaaacaaccc gacagatacg ctgatgcggc tgttggattt tctcgaaaac
     2101 atcagcctcc gctccgccta tctcgccttc ctcaacgaac atccgcaaac cttggcacaa
     2161 ctggcgcaga ttatgagcca aagctcgtgg gtggcggcgt atctgaacaa atatccgatt
     2221 ttgttggacg aactcatcag cgcgcagctt ttggataccg cgtttgattg gcaggcactc
     2281 gccgccgccc tttcagacgg catcgaagcc tgcggcggcg ataccgaagc gcaaatggac
     2341 acgctgcgcc atttccaaca cgcccaagtc ttccgcctcg ccgtccaaga cctcgccgga
     2401 ttgtggacgg tagaatccct ctctgaccaa ctctccgccc tcgccgacac cgtcattgcc
     2461 gccgcccttt cgtgcgcgtg ggcggatatg cccaaaaaac accgcgacac cccgcaattc
     2521 gccgtcatcg gctacggcaa gctcggcggc aaagaactcg gctacgcctc cgacctcgac
     2581 ttagtctatc tttacgacga tccccatccc gaagcaggcg acgtgtacag ccgcctcgcc
     2641 cgccgcctga ccaaccggct ttccgccgcc accggcgcgg gcagcctcta cgaaaccgac
     2701 ctgcgcctgc gccctaatgg cgacgccggt ttcctcgcgc acagcatcgc cgcctttgga
     2761 aaataccagc gtgaaaacgc atggacatgg gaacaccagt ccctcacccg cgcccgcttc
     2821 atctgcggca cacccgaaat tcagacggca ttcgaccgca tccgcaccga aatgctgact
     2881 gccgaacgcg accaaaccgc cttggcaggc gaaatcatcg aaatgcgcga aaaaatgttc
     2941 cccacccatc cgcctgccga cagcaacgtc aaatacgcgc gcggcggcgt ggtcgacgtc
     3001 gaatttatcg tccaatatct gatacttgcc catgcccgcc agtatccgca actcttggac
     3061 aactacggca acatcgccct attgaacatc gccgccgact gcggtttgat tgacaaaacc
     3121 ctcgccggac aaagccgcac cgcctaccgc ctctaccgcc ggcagcagca caacaccaaa
     3181 ctgcgcgacg cggcaaaaac cgaagtaagc gacgaattgc tgtcccatta cggcaatgtc
     3241 aggaaattgt ggcgggaagt gttcggggag gaagcggcaa ccgcttgaac aaaaaatgcc
     3301 gtctgaagcc tgacgattcg ggtttcagac ggcatttgtt tttggctgtg tctgaacaat
     3361 cggtacgatt tcggttgttt gcaaattgaa aggcggttat tcctatgttc aggcggcaaa
     3421 ccgaaatatt ttccccaacc gatagggcga tatcaaagcc tattcgaata ccgcctttac
     3481 ggggaagggg ctttccgagc ttgaccatgg tcaggctcgg caaatcagcc cgcatcctgt
     3541 cgatgcgtcc gtccaaatcg gtttggaacg ccgcaatgtg atatccgcct atcaaggttt
     3601 tgcgtgcgtg tgtcggcgag ggctttgcca aaaccgaaat caaaatcaag ttgtcaggca
     3661 tccttccgtt tctattaaaa tagcgcattc catttttcag acggcatcct tatgtttccc
     3721 gaccaatccg cccccaatct gctgcaaggc ttgaaccccg aacaactctc cgccgtaacc
     3781 tggccgccgc aatccgccct cgttttggca ggcgcaggca gcggcaaaac gcgcgtgctg
     3841 accacgcgta tcgcatggct tttgcaaagc ggacaagcca gcgtacacag cattatggcg
     3901 gtaacgttta ccaacaaggc cgccaaagag atgcaaaccc gactcggcgc aatgattccc
     3961 atcaacgtcc gcgctatgtg gctcggcacg ttccacggtt tgtgccaccg ctttttgcgc
     4021 ctgcaccacc gcgacgcagg cctgccgtct tcctttcaaa tcctcgacag cggcgaccag
     4081 ctttccctca tcaaacgcct gctcaaaagc ctcaacatcg ccgaagaaat catcgcgccg
     4141 cgttcgctgc aaggctttat caacgcgcaa aaagaatccg gtttgcgcgc ttccgtcttg
     4201 ggcgcgcccg atccgcacac aagccgcatg attgggtgct acgccgaata cgacaaaatc
     4261 tgccaacgcg aaggcgtggt cgatttcgcc gaactcatgc tccgcagcta cgaaatgctg
     4321 caaagtaacg aaatcctgcg ccggcactac caaaaccgct tcaaccacat tctggtcgac
     4381 gagtttcaag acaccaacaa actgcaatac gcctggctca aactcatggc gggcggtaac
     4441 gcagcggtat ttgccgtcgg cgacgacgac caaagcattt accgcttccg cggcgcaaac
     4501 gtcggcaaca tgaccgcact gatggaagaa ttccacatcg acgcgcccgt caaactcgaa
     4561 caaaactacc gctccgtcgg caacatcctc gccgccgcca acgccgttat cgaaaacaac
     4621 gacgaacgtc tcggcaaaaa cctgcgcacc gacgccgaag caggcgacaa aatccgctac
     4681 tactccgcct ttaccgacct cgaagaagcc cggttcatcg tggatgaaac caaagccctc
     4741 gaacgcgaag gctgggattt ggacgaaatc gccgtcctct accgcagcaa cgcccaatcg
     4801 cgcgtcatcg aacaaagcct gttccgcagc ggcatcccct acaaaatcta cggcggcctg
     4861 cgcttttacg aacgccaaga aatcaaacac gccctcgcct acctgcgcct cgccgtcaat
     4921 cccgacgacg acaacgccct cttgcgtgtc atcaacttcc caccgcgcgg catcggcgcg
     4981 cgcaccgtcg aaaaccttca gacggcctca aacgaacaag gcatcaccct ctggcaggca
     5041 gcctgcaacg ccggcgcgaa agccgccaaa gtcgccgcct tcgtccgcct gattgaagcc
     5101 ctgcgcaacc aagtcggaca aatgcacctg tccgaaatca tcgtcggcat cctcaaagac
     5161 agcggtctga ccgagcacta ccgaacccaa aaaggcgaca accaagaccg cctcgacaac
     5221 cttgacgaac tcgtcaacgc cgccatcgag ttcaaacccg aagacagcaa ctttgaaacc
     5281 ctgcccgaaa acatttcaga cgaccccgcc ttccccatcc tcgccttctt gagcaacgcc
     5341 gccctcgaat ccggcgaaaa ccaagcaggg gcaggcgaaa aagccgtcca actgatgacc
     5401 gtccacgccg ccaaaggctt ggaattcaac gccgtcttcc tcaccggtat ggaagagggg
     5461 cgtttcccca gcgaaatgag ccttgccgag cgcggcggcc tcgaagaaga acgccgcctc
     5521 atgtacgtcg ccatcacccg cgcccgcaaa cgcctctaca tcaccatggc acagcaacgc
     5581 atgctgcacg gacaaaccca attcggcatc gcatcccgct tcgtcgaaga aatcccgcca
     5641 gaagtattgc actacctgtc cgtcaaaaaa cccgccttcg acagctacgg caacacgcgc
     5701 caaaccaccg tacaacggga caaaatcatc gatgacttca aacagccgca aacctacgca
     5761 ggcttccgca tcggacaaaa cgtccgtcac gccaaattcg gcaccggcgt gattatcgat
     5821 gccgcagaca aaggcgaatc cgcccgactg accatcaact tcggcaaaca gggcgtgaaa
     5881 gagctggata ccaagtttgc gaaattggaa gcgatgtaaa tttgaaatgc aggccggata
     5941 ttcgtatccg acaaaaaaac atttgacgcg tttatcgttt ccgaaaaacc gctgttggaa
     6001 atgtcggatt cgagaatccg acctacggca aaaaaccgta gcaaggacaa ggcaaatagt
     6061 cgtctgaaaa cgggaagggc aattttgccg cagccgccgc cgtcattccc gcgaacctac
     6121 attccgtcat tcccacgaaa gtgggaatcc ggaacgcaaa atctaaagaa accgttttat
     6181 ccgataagtt tccgcaccga caggtctgga ttcccgctta tgcgggaatg acggcggcgg
     6241 gaatcggcg
//
LOCUS       DAXDXW010000101         6236 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXDXW010000101 DAXDXW010000000
VERSION     DAXDXW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.102"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(52..742)
                     /locus_tag="ACFB64_001951"
                     /pseudo
     CDS             complement(52..742)
                     /locus_tag="ACFB64_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1444..2286)
                     /locus_tag="ACFB64_001952"
     CDS             complement(1444..2286)
                     /locus_tag="ACFB64_001952"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC1210247.1"
                     /translation="MLICNPYEVVIHGTTSSGKIFRPGDWAERLCGILSSFTKDNRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNDPANERVLLAQAIEEKHAAEKTQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDIGRFTEQINKTQRPQGYRLLGIFEEGKHNAVAVCGFREACTLAGGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKISAETGVTKIHLNVHVNHDRTDAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            2521..3441
                     /gene="pyrB"
                     /locus_tag="ACFB64_001953"
     CDS             2521..3441
                     /gene="pyrB"
                     /locus_tag="ACFB64_001953"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC1210248.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     VQRHPKDGAARVAAEFSCVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELEEADCRYRALGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRTDEIHPDVD
                     AAPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            3451..3909
                     /gene="pyrI"
                     /locus_tag="ACFB64_001954"
     CDS             3451..3909
                     /gene="pyrI"
                     /locus_tag="ACFB64_001954"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC1210249.1"
                     /translation="MEAQKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLTLPDEI
                     AEVFRCPNPNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYNRDSVAEA"
     gene            3972..4637
                     /locus_tag="ACFB64_001955"
                     /pseudo
     CDS             3972..4637
                     /locus_tag="ACFB64_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5042..>6236)
                     /locus_tag="ACFB64_001956"
     CDS             complement(5042..>6236)
                     /locus_tag="ACFB64_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004466861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC1210250.1"
                     /translation="AAGMAFSTQTLAANLEVRPDAPQRYTVKQGDTLWGISGKYLYSP
                     WQWCRLWGANRDQIHNPDLIYPGQVLVLRYVGGEPRLDLEQTDGIPVVKISPDKEVSG
                     YGIPAIDVNFYRVFMQHPQIVSRKETAAAPRLLSGPEGRLLYTKDARVYTKGLKEPGR
                     YLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSALEQRSKQAEERLKDNEYYTR
                     THPLITPVRTRSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMVPHEPSRPVQAKIVS
                     VFEGVGVGGQFKTITIDKGGDDGLDKGAVLSLYKRKKTMQVNLSNNLTEEPKSRDTVE
                     LISTPAEEVGLAMVYHTAPKLAYAIILENISDISEGDTAANPGRDLDNMPDQGRARVD
                     SDPFQ"
BASE COUNT         1490 a         1524 c         1739 g         1483 t
ORIGIN      
        1 cgccgccttg tcctgattac tatataaaat aaatgggcaa aaatcggttt attatcgttt
       61 ttgccgcatt tggatttgtt ctaccgtaaa acgtgtttga cgaacgggat ttttattaaa
      121 aaacatctga tttctaacaa aatcagtatt ttttggcacg atggctaaaa tttttccttc
      181 catttcgcca tcacgtgttt tccatgcgct caagaattgt gatttgctcg ttgagacgtg
      241 ccccagcgat ggatcagcca gcaaaacagt ttctccgtta ataccgttca ataccgaaaa
      301 atggttgttt ttacggtatt ttaaatacac aattacagga atttttagtt gtaccaactg
      361 ttcaaatggc aaagcataac cttgtgcttc aaaacccagt tcgggcatta tgcgttgcat
      421 atcatcaaaa gaagcacgca tttgggtttt atccattttg tctaagattt ccgcttcaga
      481 ataatgtctg ccataaaaat tattcagtaa cgtggcaatc gaagccgcgc cgcaagaaaa
      541 atccaaatct tgttttacta tgccggaatc tcgccgtgct ttccaactcc gtacatggat
      601 gttttggtaa gaagcggggg tcaacaaaca taggccaagc aaaaactata tttggggcga
      661 aaccaatcaa agccgcataa tttatcaatt tataaagatt ttttatcata atatgtatac
      721 gcggaataaa cgcatgaaac ataaaaaaat aaaatcatat gcaattttat tgcatgaata
      781 aatatgaata aaatagataa tgatgggagt aaatacgcca tgtattttgg aagtttaaat
      841 ttattaataa taaaataatt tatcgtagcg caaataaatc ccaaaattgg aacgattaga
      901 aaaaaaatta atacacccat aatcatgcaa cctatattaa taaatagcta actaattcta
      961 ttacagaaat tagttagctg attaaaagtg attaacaatt atagtggatt aacaaaaatc
     1021 aggacaaggc gacgaagccg cagacagtac aaatagtacg aaaccgattc acttggtgct
     1081 tcagcacctt agggaatcgt tctctttgag ctaaggcggg gcaacgccgt actggttttt
     1141 gttaatccac tataaatagc gagcatcgat tcacggtgcg ctttagtgca aagcgtacaa
     1201 cgcgagcctg aaccaccagc cgcaatagga aaagaaagcc gatagagtgc aatgcttgcc
     1261 aacgcgcaag cgagcttgca agaacgcttg gctcaacgag agcaggcaag acagaaagca
     1321 ggataggaga ggcaacgcaa aggtctcggg ctttgatttc gccgtaaacc tgagtgccgc
     1381 cttgtccgga cggggtggtt caggcggcgg gtgccgacag ggtgcagatg aggaggagta
     1441 ttttcatttg gggtcgcaac ggaagtggta ggcgcagatt tcaaaaccgt ttttgaaata
     1501 caggcggtgc gcgtcggtac ggtcgtggtt gacgtggacg ttaaggtgga ttttggtaac
     1561 ccctgtttcc gcgctgattt tgcggacttc ttccaaaagg cgcgaggcgt agcctttgcg
     1621 gcggctttgc ggcagggtaa cgatgtcgtc gatgtggatg tggcggccgc cggcgagggt
     1681 gcaggcttcg cggaagccgc agacagcgac ggcgttgtgt ttgccttctt caaaaatgcc
     1741 cagcaggcgg tagccttggg ggcgttgggt tttgttgatc tgttcggtaa agcggccgat
     1801 gtcggtcagg gcggaacgca aaacactcaa ggctgcaaag gcggtggcgg tgtcgtccgc
     1861 gccgatttcg cgtaaaacgt aggatgcgcc cgaggcggtc tgttcctgtg ttttctcggc
     1921 ggcgtgtttt tcttcgattg cctgtgccag caggacgcgt tcgttggcag ggtcgttttg
     1981 tccgccctgt tcgcgttctt cgagcagggc tttgcagtcg atgacgcgca ggtcgttgtc
     2041 ggcggcaaag tccatcagga agcggaacat ctgcggattg tcggtttcca gttttttatc
     2101 gacggcgacg cagcggatgt tgtccaccag catggggcgc acccatttcg aataggagag
     2161 gcggttgtct ttggtgaacg aggacaggat gccgcacagg cgttccgccc agtcgccggg
     2221 gcggaaaatc ttgccggaac tcgttgtgcc gtggatgacg acttcgtagg ggttgcagat
     2281 taacatggcg gcttcctgaa aagaaatgtc tggcgcgatt ataccttatg cttatgcggg
     2341 cgtgtttgga tatgccgtct gaaaaataca ggattcgtgc ggtaaaactt tgtggaggca
     2401 aatgtgcgat aatacgcgcc gtgtcgccgc ttttgcgaag ctgttccgca aacatacggg
     2461 cggcgtggac gtataaccgg atgcccgcct gacgcgggtt ttttacggaa ggggggcaaa
     2521 atgcctaatc cgctttacag acagcacatc atctccattt cggatttgtc gcgcgaacag
     2581 ttggaatgcc tgcttcagac ggcattgaag ctgaaggcgc atccgcgcgg cgatttgttg
     2641 gaaggcaaac tcatcggttc gtgctttttc gagccgtcca cgcgcacgag gctgtcgttt
     2701 gaaacggcgg tgcagcgttt gggcggtaag gtcatcggct tttcggacgg cgcgaatacc
     2761 agtgccaaaa aaggcgaaac gcttgccgat accgcccgta tcatttccgg ctacaccgac
     2821 gcgattgtcc aacgccatcc caaagacggc gcggcgcgcg tggcggcgga gttttcgtgc
     2881 gtccccgtca tcaacgccgg cgacggcacg aaccagcacc ccagccaaac cttgttggac
     2941 ctggtaacga tttatgaaac gcaggggcgt ttggacaagc ttaaaatcgc catggcgggc
     3001 gacttgaaat acgggcgcac cgtgcattcg ctgtgtcagg cgttgaaacg ctggggctgc
     3061 gaatttgcct tcgtctcgcc gcccagcctc gcgatgcccg actacattac cgaagagttg
     3121 gaagaagccg actgccgata ccgcgccctc ggcagtttgg aagaggcggc ggaatgggcg
     3181 gacatcctgt atatgacccg cgtccagcgc gaacgtttcg acgaacagga atttgccaaa
     3241 atccaaggca aattcaacct cgaagcgtct atgctcgccc gcgccaaacc gaacctgcgc
     3301 gtgctgcacc ccctgccgcg cacggacgaa atccaccccg atgtcgatgc cgcgccgcac
     3361 gcctattatt tcgagcaggc gaccaacggc gtttatgcgc gtatggcgat attgtcgctg
     3421 gtgttgaacg aagaagtgtg aggaaccgat atggaagcgc aaaaactcag tgtcgaagcc
     3481 atcgaaaaag gcacggttat cgaccatatt cccgccggca gggggctgac catcctgcgc
     3541 cagttcaaac ttttgcacta cggcaacgcg gtaaccgtgg gcttcaacct gcccagcaaa
     3601 acccagggca gcaaagacat catcaaaatc aaaggcgtgt gcttggacga taaagccgcc
     3661 gaccgcctcg ccctgttcgc ccccgaagcg gtggtcaaca ccatcgacaa tttcaaggtc
     3721 gtacagaagc ggcatttgac cctgcccgac gaaatcgccg aagtgttccg ctgcccgaat
     3781 cccaattgcg ccggccacgg cgagccggtc aaaagccggt tttatgttaa gaaacacaac
     3841 gggcagacgc ggctgaaatg ccactactgc gaaaaaacct acaaccggga ttcggtggcg
     3901 gaagcctgac ggattcccgc agcccggcgg cggaattttg cctgccgcct gttttgccaa
     3961 tctgaaatgg aatgatgatg cacgcttctg tccaaagtcg tttcgcaccg atactttatg
     4021 ttttgatttt ctttgccggt tttttgaccg cgcaaatctg gttcaatcag aaagcctata
     4081 ctgaagagct gcctccgctt ctgtccgcat tgtccgccgt cgcgctggtg tggctggcgt
     4141 gggcgttcgt gtcggtgcgt tcaaaggcta aggcagaaaa gttctaccgc gaaaaaatga
     4201 tacagaacga aagcataccc ccgtcctgca cgcttctttg caacacttgg aacacaagcc
     4261 gcaaatgctc gccctgctgg tcaaaaacca cggcaaaggc atggcggaac aggtcaggtt
     4321 caaggcggaa gtgctgcccg acgacgaaga cgcgcgcacg attgccgccg agttggcaaa
     4381 aatggatatg ttcgcattgg ggacggacgc ggtcgcctcg ggcgaaacct atgggcgcgt
     4441 gttcgccgat attttcgagt tgtcggcggc tttggaaggg cgcgcgttca aagggatact
     4501 gaaactgacg gcggaatata aaaaacatct tcggcgatgc ctgccgttcg gaaacggcgt
     4561 tggatttggg cgcgctcaat caggcgttga gggaaatctc gaaaacgccg gaaaagccta
     4621 agcagatatt ttattgaagg tgtcccgatg tatggggagc gcacgggtgg cgcggcggca
     4681 aagcggcaag attgggtttg gatacccggt tggttttata gtagattaac aaaaatcagg
     4741 acaaggcggc gggccgcaga cagtacaaat agtacggaac cgattcgccc ggtgcttgga
     4801 caccttaggg aatcgttccc tttgagccgg ggcggggcaa cgccgtaccg gtttttgtta
     4861 attcgctata tctgtctgca agggagacag ccgctgtgat ttccgttcgg gcggaaaccg
     4921 attccatcgg ataaaaggca ttttgtccga ctaaatcagg gttttgcatc aacgggatat
     4981 gaaaacacag cctaaataaa aatgccgtct gaatgctcca gacggcattg ttgccgaggg
     5041 gttattggaa agggtcggaa tcgacgcggg cgcggccctg atccggcata ttgtccaaat
     5101 cccgtcccgg attggcggcg gtgtcgcctt cggaaatatc ggagatgttt tccaaaataa
     5161 tggcgtaagc cagtttcggc gcagtgtggt aaaccattgc caagcccact tcttcggcag
     5221 gtgtcgaaat cagctcgacg gtatccctgc ttttaggttc ttcggtcaaa ttgttggaga
     5281 ggttgacctg catcgttttc ttgcgtttgt aaaggcttaa aactgcgcct ttgtccaaac
     5341 cgtcgtcccc gcctttgtct atcgtaatgg ttttgaactg tccgccgacc cccacgcctt
     5401 caaacacgga aacgattttt gcctgaaccg ggcgggacgg ttcgtgcggc accatattga
     5461 agcggtcggt gtcttccggc attttcatca ggtagtcgcc ctgctgtatt tcggaaatgg
     5521 cggtttcgac caccagcggc tgtatcgaac gggtgcgcac cggggtaatc aaaggatggg
     5581 tgcgggtgta gtattcgttg tctttcagcc gttcttcagc ctgtttcgag cgttgttcga
     5641 gggcggagtc ggtatagtcg agggagcgca cgatgccgct gaatgcgact tcttgtccga
     5701 ggaatttacc cgtatccgga tcggtgatgt ttttattgat tcggtaggtc agataacggc
     5761 ccggctcttt caggcctttg gtgtaaaccc tggcgtcttt ggtgtacagc agcctgcctt
     5821 ccgggcccga gagcaggcgc ggcgcggcag cggtttcttt gcgggaaacg atttgcgggt
     5881 gctgcataaa gacgcggtag aagttgacat cgatggcggg aataccgtat ccggacactt
     5941 ccttatccgg actgattttg acgacgggga tgccgtctgt ctgttccaaa tcaaggcgcg
     6001 gttcgccgcc aacgtagcgc aacaccaata cctggcccgg ataaatcagg tcgggattgt
     6061 ggatttgatc ccggttcgcg ccccacaggc ggcaccattg ccacgggctg tacaggtatt
     6121 tgcccgaaat accccacagg gtgtcgccct gtttgaccgt gtagcgttgc ggcgcatccg
     6181 ggcgcacctc cagatttgcc gccaaagttt gtgttgagaa tgccatacct gccgcg
//
LOCUS       DAXDXW010000102         5712 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXDXW010000102 DAXDXW010000000
VERSION     DAXDXW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.103"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(363..518)
                     /locus_tag="ACFB64_001957"
     CDS             complement(363..518)
                     /locus_tag="ACFB64_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210251.1"
                     /translation="MPSESPSDGIGFPKHSAPLSSCRKQTPIRFAANAQKTQCRLKRQ
                     TGFRRHR"
     gene            complement(535..2331)
                     /locus_tag="ACFB64_001958"
     CDS             complement(535..2331)
                     /locus_tag="ACFB64_001958"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC1210252.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVVTANEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIQHPDDLLDQVWTSRPAIPAETVFIH
                     DHAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDSAVLFTEQCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESARLIEGINPSTFFKSVKSEADIARIREAMEQDGAALCGFFAEFEDIIGKGG
                     SLTEIDVDTMLYRHRSARPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVADPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2790..4043
                     /locus_tag="ACFB64_001959"
     CDS             2790..4043
                     /locus_tag="ACFB64_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC1210253.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QLALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSCVYLWVFLILCIASATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLVAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRIRPSGYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGREKTGNAELFAWN
                     IWVAGSGLAVIFWFDGAMAELLKFAMIAAFVSAPVFAWLNYRLVKGDKRHRLTAGMNA
                     LAIVGLLYLAGFAVLFLLNLTGLLA"
     gene            4200..5711
                     /gene="lysS"
                     /locus_tag="ACFB64_001960"
     CDS             4200..5711
                     /gene="lysS"
                     /locus_tag="ACFB64_001960"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC1210254.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLNNIRQQRNAYPNDFKRDS
                     FAADLQAQYGEIGKEELDPQAVPVKIAGRMMLKRQMGKASFATIQDVTGQIQLYLNNK
                     GVSQEVLDGFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDLQTIRDVILFPQMRPE"
BASE COUNT         1182 a         1511 c         1687 g         1332 t
ORIGIN      
        1 cctccccggc cctcccccac gggggaggga gcagattgcg gcagatttgg cggttgcagg
       61 cggtttgatt tgcgagggaa tcggacgcgc aggcgggaat ccggatttgt ccgcgcggaa
      121 acttatattt cgtcattcca ccaatcccgc ccacagccca cttttccgcg tcattcccgc
      181 gcaggcggga atccagtccg ttcggtttcg gttatttccg ataaattcct gctgcttttc
      241 attcctagat tcccactttc gcgggaatga cgggatttca gggtaaagat ggcgggagcc
      301 ggagggctgt gaagagctgt tttcgcagga atgacgatat gcggatttgc tgttttcatc
      361 cgctaccgat gccgtctgaa gcctgtttgg cgtttcagac ggcattgggt tttttgtgca
      421 tttgccgcaa aacggatcgg tgtctgcttg cggcaagacg ataacggtgc ggaatgtttt
      481 gggaaaccaa tgccgtctga agggctttca gacggcattt ttgtaccgtg ccgtttaacg
      541 cgccagcggt tcggtgcgtt tgatcagcca cgcttttgcc gcgccttcgg tcagcggctc
      601 gaggcggcgg cggacttcgg cgtggtagcg gttgacccag tcgatttcgc cgtcggtcat
      661 cagggcggtg tccatcaggc gtgtatcgat ggggcagagg gtcagggttt caaaacagag
      721 gaagctgccg aactcggttt cctgcgggtc ggcgacggct tggttggcgg cgaggttttc
      781 aatgcggatg ccccatttcc ccggacggta gaggccgggt tcgatggagg taaccatgcc
      841 ttttttcatg gcggtttcgg gcgtggcggg ggcggcgaag gcgatgcgct gcgggccttc
      901 gtggacgttg aggaaatagc ctacgccgtg gccggtgccg tgtccgtagt cgcattgcgc
      961 ctgccacagg ggtttgcggc aaatcgcgtc aatcagcggc gaggggatgt tttcggggaa
     1021 cacggcttcg gcaagcgcga tatgggcttt gagaacgagc gtgttgtcgc gtttttgttc
     1081 tgctgtgggc gtgccgacgg ggacgacgcg ggtgatgtcg gtcgtgccgc ctttgtattg
     1141 cgcgccggag tcgatgagca gcagaccgtt gccgctgatg gtgctgtggc tttcgggtgt
     1201 tgcgctgtaa tgcggcaggg caccgttggc gttgaagcct gcgatggtgt cgaaactcaa
     1261 ggacacaaaa cccgggcgcg cgctgcggtg gcggtaaagc atcgtatcca cgtcgatttc
     1321 ggtcaggctg ccgcctttgc cgatgatgtc ttcaaactcg gcgaagaagc cgcacaacgc
     1381 cgcgccgtct tgttccatcg cttcgcggat gcgggcgatg tcggcttcgg atttgacgga
     1441 tttgaagaag gtggacgggt tgatgccttc aatcaggcgc gcgctttcgg gcaggcgcac
     1501 gagcgtactg acggcggttt tgttcggctc gatgagcagc gcgccgccga tttgcgcgag
     1561 tttgtcggca acttgggcgt aaggctcgac cgtgatacct gcggtttgca gcgcggcggc
     1621 ggcttcggcg ttcagacggc attgttcggt aaacaggacg gcactgtctt tgccgatcag
     1681 caggaaggac acgaaaacag ggttgaaagg cacgtcgctg ccgcgcaggt tggtcagcca
     1741 agcgatgtcg tcaagcgagg aaaccaagtg gtaatccgcg cctttttccg ccataactgc
     1801 gcgcacgcgg gcgagtttct cggcggcggt ttcggagacg taggcgtggt cgtggatgaa
     1861 cacggtttcg gcggggatag cgggacggct tgtccacact tggtcaagca ggtcgtcggg
     1921 gtgttggatg cggatgtttt tggcggcgag cgattgcgcc aaagtgcgtt tgccggtgag
     1981 cgacaccata tcggaaggga tgccgacggc agcgttttcg ggcaggtttg ccgcgagcca
     2041 ttcgttgtac ggcggcactt gcccgctttt ttgcagctca atgccgctgc cggaaagctg
     2101 tttggcggct tgttcccaat agcggctgtc cacccatacg cccgcttcat tggcggtaac
     2161 gacgaatgtg ccgaccgagc cggtaaagcc tgacaattcg cgccgcgcct gccaatgctc
     2221 gggcaggtat tcggataggt gggggtcggc ggaagggatg acgagtgcgt ccaagccttg
     2281 cgccttcatg gcttcgcgca atgcggacag gtaattcgat acggtattca taacagttcc
     2341 tccaatcggg tttcgcggct tcagacggca tggacggaaa tctgaaatgc cgtctgaaaa
     2401 acataaccat attatcaaat cgccagcctg ctgcaaaacg gacgggctga tcggatggtg
     2461 cgtgtttgca cgaaaaaaag cgatgtaagg ttgtttgttg taaaatttca ggcataaaat
     2521 gtcgtttcat gccttctttt ggaagtaata cggaaaatat agtcaggttt cagcatgaag
     2581 ttctaatagt gtgtgttggc aggtatttgg attacgttac aatacgcgct ttcatcaaaa
     2641 tttccctacc gggcggtatc ctgtttttga agacggtagg atttgggttt gtttggggtc
     2701 aatcccgccc aaattggagc aatagatagg gctgtgtccg aatatgcggc tctgtatgtt
     2761 gaaacacagt taaaaatacg gaagacttta tgtccgaaca acatatttcg acttggaaaa
     2821 gcaagatcaa cgcattggga ccggggatta tgatggcttc ggcggctgtc ggcggttcgc
     2881 acctgattgc ctcgacgcag gcgggcgcgc tttacggctg gcagctcgcg ctcatcatca
     2941 tcctgaccaa cctcttcaaa tacccgtttt tccgcttcag cgcgcattac actctggaca
     3001 cgggcaagag tctgattgaa ggttatgccg agaaaagctg cgtttatttg tgggtttttt
     3061 tgattttgtg tatcgcctcc gccacgatta acgcgggcgc ggtcgccatt gtaaccgccg
     3121 ccatcgtcaa aatggcgatt ccctcgctga tgtttgatgc cggcacggtt gccgccttga
     3181 ttatggcatc ctgcctgatt attttggtga gcggacgtta ccgcgctttg gatcgcgttt
     3241 ccaaaatcat catcgttact ttgagtatcg ccacgcttgt cgccgccggc atcgctatgt
     3301 cgcgcggtat gcagatgcag cccgatttta tcgagccgac accgtggacg cttgccggtt
     3361 tgggcttcct gatcgcgctg atgggttgga tgcccgcgcc gattgagatt tccgccatca
     3421 attctttgtg ggtaaccgaa aaacaacgca tccggccttc cggctaccgc gacggcattt
     3481 tcgatttcaa cgtcggttat atcgccagtg cggttttggc tttggttttc cttgcactgg
     3541 gcgcgtttgt gcaatacggc aacggcgaag cagtgcagat ggcgggcggc aaatatatcg
     3601 ggcaattgat taatatgtat gccgtaacca tcggcggctg gtcgcgcccg ctggtggcgt
     3661 ttatcgcgtt tgcctgtatg tacggcacga cgattaccgt tgtggacggt tatgcgcgcg
     3721 ccattgccga gcccgtgcgc ctgctgcgcg gcagggaaaa aaccggcaac gccgaactct
     3781 ttgcctggaa tatttgggtg gcgggcagcg gtttggcggt gattttctgg tttgacggcg
     3841 caatggcgga actgctcaaa tttgcgatga ttgccgcctt tgtgtccgcc cctgtgttcg
     3901 cctggctcaa ctaccgcctc gtcaaagggg acaaacgcca caggcttacc gccggtatga
     3961 acgcccttgc cattgtcggc ctgctctacc tggccgggtt tgccgttttg ttcctgctga
     4021 acctgacggg gcttttggca tagtttccaa aacacggatg ccgtctgcaa gattttcaga
     4081 cggcatcgcc ctttcttgga aaaaaacacc gacatcgcgt aaaatacgcg caaattttgt
     4141 gattcggtca ggtcgtctga aacagactgc gcgcctgatt tattttttcg gaaaacccta
     4201 tgagcgaaca aaaccatccg caaaccgagc cgcagttgga cgaaaaccaa atcatcgccc
     4261 tgcgccgcga aaaactgaac aacatccgcc aacagcgcaa cgcttacccc aacgatttca
     4321 agcgcgacag ctttgccgcc gatttgcagg cgcaatacgg cgaaatcggc aaagaagaac
     4381 tcgacccgca ggccgttccc gttaaaattg ccggccgcat gatgctcaaa cgtcaaatgg
     4441 gcaaagccag cttcgccacc attcaagacg ttaccggtca aatccagctt tatctgaaca
     4501 acaaaggcgt gagtcaggaa gttttggacg gcttcaacca ctgggacttg ggcgacatcg
     4561 tcggcgcgga aggcactttg ttcaaaacca accacggcga actaaccgtg cgtgtgtccg
     4621 acatccgcct gctgtccaaa tccctgcgtc cgctgcccga caaacacaaa ggcttgagcg
     4681 atcaggaaac caaataccgc caacgctacg tcgatttgat tgccaatgaa gaatcgcgca
     4741 atacctttat caaacgcagc caaatcatcc aatctgtgcg taattttatg gtgggcgagc
     4801 attatctcga agtcgaaacc ccgatgatgc acccgattcc cggcggcgcg acagcgaaac
     4861 ccttcgttac ccaccacaat gccttggaca ttccgctcta cctgcgcatc gcgcccgagc
     4921 tgtatctgaa acgcttggtg gtcggcggct tggaacgcgt gtttgaaatc aaccgcagct
     4981 tccgtaacga gggcatgtcc gtgcgccaca accccgaatt tacgatgatt gaattttacg
     5041 aagccttctc cgactacgaa cgtatgatgc agatggcgga agacatcatc cgcaacgcat
     5101 cgcgcacggt aaacggcacg gcaaacatca cttacaacgg caaagaagtc gatttggaaa
     5161 gcccgttcga acgtctgacc atcctcgaag ccatcaaaaa atacaatccg cactacaccg
     5221 acgagcagtt gaacgatgcg gaatggctga aaaaagaaat cgtcaaacac ggcgaaagcc
     5281 tgccgccgtc cccgggcatc ggcagcctgc aactcgccct gtttgaaggt tgcgccgagg
     5341 gcaaactgtg gaatccgacc tttattgtcg attacccggt cgaagtttcc ccgttggcgc
     5401 gcgcttcgga tacgaaacaa ggtctgaccg aacgcttcga gcttttcgtt gtcgggcgcg
     5461 aactggcaaa cggctattcc gagttgaacg accccgaaga ccaagccgaa cgcttcaaat
     5521 cgcaagtggc gcaaaaagat gcgggcgacg acgaagccat gcactacgat gccgactaca
     5581 tccgcgcgat ggaattcggt ttgccgccga ccggcggttg cggcatcggc atcgaccgct
     5641 tagtgatgct gctgaccgat ttgcaaacca tccgcgatgt gattctgttt ccgcagatgc
     5701 gtcccgagta at
//
LOCUS       DAXDXW010000103         5512 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXDXW010000103 DAXDXW010000000
VERSION     DAXDXW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.104"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(27..140)
                     /gene="rrf"
                     /locus_tag="ACFB64_001961"
     rRNA            complement(27..140)
                     /gene="rrf"
                     /locus_tag="ACFB64_001961"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(237..3128)
                     /locus_tag="ACFB64_001962"
     rRNA            complement(237..3128)
                     /locus_tag="ACFB64_001962"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3475..3550)
                     /locus_tag="ACFB64_001963"
     tRNA            complement(3475..3550)
                     /locus_tag="ACFB64_001963"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3515..3517),aa:Ala,seq:tgc)
     gene            complement(3556..3632)
                     /locus_tag="ACFB64_001964"
     tRNA            complement(3556..3632)
                     /locus_tag="ACFB64_001964"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3596..3598),aa:Ile,seq:gat)
     gene            complement(3733..5273)
                     /locus_tag="ACFB64_001965"
     rRNA            complement(3733..5273)
                     /locus_tag="ACFB64_001965"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1181 a         1638 c         1188 g         1505 t
ORIGIN      
        1 gatatccggg ggttttctga aagggtgttt ggcagtgacc tactttcgca tggaagaacc
       61 acactatcat cggcgctgag tcgtttcacg gtcctgttcg ggatgggaag gcgtgggacc
      121 aactcgctat ggccgccaaa cttaaactgt tgcaaatcgg taaagcctta atcaatatat
      181 tcggtaatga ctgaatcagt cagtaagctt ttatctcttg aagtccttca aatgatagag
      241 tcaagcctca cgggcaatta gtatgggtta gcttcacgcg ttaccgcgct tccacacccc
      301 acctatcaac gtcctggtct cgaacgaccc tttagtgcgg ttaaaccgca agggaagtct
      361 catcttcagg cgagtttcgc gcttagatgc tttcagcgct tatctcttcc gaacttagct
      421 acccggctat gcaactggcg ttacaaccgg tacaccagag gttcgtccac tccggtcctc
      481 tcgtactagg agcagccccc gtcaaacttc caacgcccac tgcagatagg gaccaaactg
      541 tctcacgacg ttttaaaccc agctcacgta ccactttaaa tggcgaacag ccataccctt
      601 gggaccgact acagccccag gatgtgatga gccgacatcg aggtgccaaa ctccgccgtc
      661 gatatgaact cttgggcgga atcagcctgt tatccccgga gtacctttta tccgttgagc
      721 gatggccctt ccatacagaa ccaccggatc actatgtcct gctttcgcac ctgcccgact
      781 tgtcggtctc gcagttaagc taccttttgc cattgcacta tcagtccgat ttccgaccgg
      841 acctaggtaa ccttcgaact cctccgttac gctttgggag gagaccgccc cagtcaaact
      901 gcctaccatg cacggtcccc gacccggatg acgggtctgg gttagaacct caaagacacc
      961 agggtggtat ttcaaggacg actccacaga gactggcgtc tctgcttcca agcctcccac
     1021 ctatcctaca caagtgactt caaagtccaa tgcaaagcta cagtaaaggt tcacggggtc
     1081 tttccgtcta gcagcgggta gattgcatct tcacaaccac ttcaacttcg ctgagtctcg
     1141 ggaggagaca gtgtggccat cgttacgcca ttcgtgcggg tcggaactta cccgacaagg
     1201 aatttcgcta ccttaggacc gttatagtta cggccgccgt ttaccggggc ttcgatccga
     1261 tgcttgcaca tcttcaatta accttccggc accgggcagg cgtcacaccc tatacgtcca
     1321 ctttcgtgtt ggcagagtgc tgtgttttta ataaacagtc gcagccacct attctctgcg
     1381 accctccggg gcttacggag caagtcctta accttagagg gcataccttc tcccgaagtt
     1441 acggtatcaa tttgccgagt tccttctccc gagttctctc aagcgcctta gaattctcat
     1501 cctgcccacc tgtgtcggtt tgcggtacgg ttcgattcaa actgaagctt agtggctttt
     1561 cctggaagcg tggtatcggt tgcttcgtgt ccgtagacac tcgtcatcac ttctcggtgt
     1621 taagaaaacc cggatttgcc taagtcttcc acctaccggc ttaaacaagc tattccaaca
     1681 gcttgccaac ctaaccttct ccgtccccac atcgcatttg aatcaagtac aggaatatta
     1741 acctgtttcc catcgactac gcatttctgc ctcgccttag gggccgactt accctacgcc
     1801 gatgaacgtt gcgtaggaaa ccttgggctt tcggcgagcg ggcttttcac ccgctttatc
     1861 gctactcatg tcaacattcg cacttctgat acctccagca cactttacaa tgcaccttca
     1921 tcagcctaca gaacgctccc ctaccatgcc ggtaaaccgg catccgcagc ttcggttata
     1981 gatttgagcc ccgttacatc ttccgcgcag gacgactcga ccagtgagct attacgcttt
     2041 ctttaaatga tggctgcttc taagccaaca tcctggctgt ctgtgccttc ccacttcgtt
     2101 taccacttaa tctatcattt gggaccttag ccggcggtct gggttgtttc cctcttgaca
     2161 acggacgtta gcacccgctg tctgtctccc gaggaaccac ttgatggtat tctgagtttg
     2221 ccatgggttg gtaagttgca ataaccccct agccataaca gtgctttacc cccatcagtg
     2281 tcttgctcga ggcactacct aaatagtttt cggggagaac cagctatctc cgagtttgtt
     2341 tagcctttca cccctaccca cagctcatcc ccgcattttg caacatgcgt gggttcggtc
     2401 ctccagtacc tgttacggca ccttcaacct ggccatggat agatcactcg gtttcgggtc
     2461 tacacccagc aactcatcgc cctattaaga ctcggtttcc ctacgcctcc cctatccggt
     2521 taagctcgct actgaatgta agtcgttgac ccattataca aaaggtacgc agtcacacca
     2581 ctagggcgct cccactgttt gtatgcatca ggtttcaggt tctgtttcac tcccctcccg
     2641 gggttctttt cgcctttccc tcacggtact ggttcactat cggtcgatga tgagtattta
     2701 gccttggagg atggtccccc catattcaga caggatttca cgtgtcccgc cctacttttc
     2761 gtacgcttag taccgctgtt gagatttcga atacgggact gtcacccgct atggtcaagc
     2821 ttcccagctt gttcttctat ctcgacagtt attacgtaca ggctcctccg cgttcgctcg
     2881 ccactacttg cggaatctcg gttgatttct tttcctccgg gtacttagat ggttcagttc
     2941 tccgggttcg cttctctaag cctatgtatt caacttagga tactgcacag aatgcagtgg
     3001 gtttccccat tcggacatcg cggaatcata gctttattgc cagctccccc gcgcttttcg
     3061 caggcttaca cgtccttcgt cgcctatcat cgccaaggca tccgcctgat gcacttattc
     3121 acttgaccct atcatttcaa gaacttcttt gacttcgttt acctacccgt tgactaagta
     3181 agcaaacttg aaatccctac tttgataaag cttactgctt tgttgtgtct taatcctgcc
     3241 ttttgtgttt caggattaag tcgatacaat catcacccaa atactatgtt tgttttcttt
     3301 tctcttgcga gaggttttta tcctttgcaa agaataaaaa atcaaaacaa actcattgtc
     3361 tttgtttgtt gatttcggct ttccaatttg ttaaagatcg atgcgtcgtt attctacttc
     3421 gcaaatcaaa ataagctgct aaaaacagca aacttgcttt catttgtaaa gttttggtgg
     3481 aggcaaacgg gatcgaaccg atgaccccct gcttgcaaag caggtgctct accaactgag
     3541 ctatgccccc gttcttggtg ggtctgggag gacttgaacc tccgacccca cgcttatcaa
     3601 gcgtgtgctc taaccagctg agctacaaac ccggtttccc ttcttaagcg aaccttgcct
     3661 tcactcaagc ttcttccgca tcttttcagt ttaccgataa gtgtgaatgc ctaaagcccc
     3721 ttctttctct agaaaggagg tgatccagcc gcaggttccc ctacggctac cttgttacga
     3781 cttcacccca gtcatgaagc ataccgtggt aagcggactc cttgcggtta ccctacctac
     3841 ttctggtatc ccccactccc atggtgtgac gggcggtgtg tacaagaccc gggaacgtat
     3901 tcaccgcagt atgctgacct gcgattacta gcgattccga cttcatgcac tcgagttgca
     3961 gagtgcaatc cggactacga tcggttttgt gagattggct ccgcctcgcg gcttggctac
     4021 cctctgtacc gaccattgta tgacgtgtga agccctggtc ataagggcca tgaggacttg
     4081 acgtcatccc caccttcctc cggcttgtca ccggcagtct cattagagtg cccaaccgaa
     4141 tgatggcaac taatgacaag ggttgcgctc gttgcgggac ttaacccaac atctcacgac
     4201 acgagctgac gacagccatg cagcacctgt gttacggctc ccgaaggcac tcctccgtct
     4261 ccggaggatt ccgcacatgt caaaaccagg taaggttctt cgcgttgcat cgaattaatc
     4321 cacatcatcc accgcttgtg cgggtccccg tcaattcctt tgagttttaa tcttgcgacc
     4381 gtactcccca ggcggtcaat ttcacgcgtt agctacgcta ccaagcaatc aagttgccca
     4441 acagctaatt gacatcgttt agggcgtgga ctaccagggt atctaatcct gtttgctacc
     4501 cacgctttcg gacatgaacg tcagtgttat cccaggaggc tgccttcgcc atcggtattc
     4561 ctccacatct ctacgcattt cactgctaca cgtggaattc cacctccctc tgacacactc
     4621 gagtcaccca gttcagaacg cagttcccgg gttgagcccg gggatttcac atcctgctta
     4681 agtaaccgtc tgcgcccgct ttacgcccag taattccgat taacgctcgc accctacgta
     4741 ttaccgcggc tgctggcacg tagttagccg gtgcttattc ttcaggtacc gtcatcggcc
     4801 gccgatattg gcaacggcct tttcttccct gacaaaagtc ctttacaacc cgaaggcctt
     4861 cttcagacac gcggcatggc tggatcaggc ttgcgcccat tgtccaaaat tccccactgc
     4921 tgcctcccgt aggagtctgg gccgtgtctc agtcccagtg tggcggatca tcctctcaga
     4981 cccgctactg atcgtcgcct tggtgggcct ttaccccgcc aaccagctaa tcagatatcg
     5041 gccgctcgga tagcgcaagg cccgaaggtc ccctgctttc cctctcaaga cgtatgcggt
     5101 attagctgat ctttcgatca gttatccccc gctacccggt acgttccgat atgttactca
     5161 cccgttcgcc actcgccacc cgagaagcaa gcttccctgt gctgccgtcc gacttgcatg
     5221 tgtaaagcat gccgccagcg ttcaatctga gccaggatca aactcttatg ttcaatctct
     5281 aactttttaa cttctggtct gcttcaaaga aaccgacagg acaatgttca aaacatcatc
     5341 tcgtctgtct ttcaaacagt gcgaggccca aggcactcac acttatcggt aatctgtttt
     5401 gttaaagagc gttgcgaaat tataaagtat cccttccgcc tgtctaagat atctctcgat
     5461 atttccgaca ttccgtgcta tacttttcag ttcgtccgcc gcttcggcag cg
//
LOCUS       DAXDXW010000104         5391 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXDXW010000104 DAXDXW010000000
VERSION     DAXDXW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.105"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(124..2883)
                     /gene="tbpA"
                     /locus_tag="ACFB64_001966"
     CDS             complement(124..2883)
                     /gene="tbpA"
                     /locus_tag="ACFB64_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent transferrin-binding protein TbpA"
                     /protein_id="HFC1210255.1"
                     /translation="MQQQHLFRFNILCLSLMTALPAYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVALTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIRTGRHAGEIRAHEAAGRGVQSFNRLVPVDDASTYAYFIVEEECKNGGYEKCKAKK
                     DVDGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERTQQ
                     TFDTRDMTVPAFLTKAVFDENKKYGLIRGYGKYAGDHRYGGLITNSENGAQVGAEYGT
                     GVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGSDK
                     YCRPSADKPSSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGYDRFGSDLR
                     HQDYYYQHANRAYSSKTPPQNNGKKTSPNGSNTSPNGSNTSPYWVSIGGGNVVTGQIC
                     LFGNNTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGT
                     HRTLSWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRSGDKIKAVKIDPEKSFNK
                     EAGIVFKGDFGNLEASWFNNAYRDLIVRGYEAQIKDGKEQVKGDPAYLNAQSARITGI
                     NILGKIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVG
                     SGYDQPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSG
                     YYTVKKHFTLRAGVYNLLNHRYVTWENVRQTAAGAVNQHKNVGVYNRYAAPGRNYTFS
                     LEMKF"
     gene            complement(2970..5051)
                     /locus_tag="ACFB64_001967"
     CDS             complement(2970..5051)
                     /locus_tag="ACFB64_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC1210256.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVPSKKPEARKDQGGYGFAMRFKRRNWYLQANPKEYKIPLSENDWEQTDNGDITNPS
                     KQKNIINALSGNGGESLQDSSRQGEGISKVTGYHDFKYVWSGFFYKRIGITIKRDDSS
                     SKIIEVRNGPDGYIFYKGIDPSRKLPVSGSVEYKGTWDFLTDVQENQKFTDLGNAFTK
                     SGDRYSAFSGELDYIVKKEDDKKEKHKGLGLTTEITVDFEQKTIKGKLIKNNMVINDK
                     DEPTTQYYSLEAQVTGNRFNGKAIATDKLQKNGTKQHPFVSDSSSLSGGFFGPKGEEL
                     GFRFLSDDEKVAVVGSAKTKDKNANGNTAAAGTAGAAGMSSEDTRLTTVLDAVELTLD
                     GKKIKDLDNFSDATRLVVDGIMIPLLSKDSEGGSSQADKGKNGGTDFTYTTTYTPESD
                     KKDTQTGMAANGVQTVSNAAGGTSGKTKTHYEVQACCSNLNYLKYGLLTRKTAGNTGG
                     GGNGSQAVQTAQGAQSMFLQGERTDEKEIPKDENVVYLGTWYGHIAANGTSWTGNASD
                     QQSGNRARFDVNFKDKKITGKLTAENRSEATFTIDAMIDGNGFKGTAKTGNGGFAPDQ
                     NSSTGTHKVHITSAAVQGGFYGPKAEELGGWFAYPGNGQTKNAQTSSGNGNSAGSATV
                     VFGAKRQELVQ"
BASE COUNT         1093 a         1409 c         1360 g         1529 t
ORIGIN      
        1 cacgcctgcg cgggaatgac gggtttcggg attacggtgt atcggggatg atgggaaacg
       61 gtggggattg tgtaaaaaac gccgtctgaa gcctttcaga cggcatttgc ggcgttcgga
      121 cgtttagaac ttcatttcca agctaaatgt gtagttgcgg ccgggggcgg catatcggtt
      181 gtaaacgccg acatttttgt gttggttgac tgcgccggcg gcagtttgcc gcacattttc
      241 ccaagtaaca tagcggtggt tgaggaggtt gtacacgccc gcacggaggg tgaagtgttt
      301 tttaaccgtg taataaccgg acacgtccac aatataccaa gggcgggtac ggcgcgcggt
      361 ggcttttgta ttgcggctgt tgccgttgag caaagcccgg ctgcccaaca actctgtgat
      421 ttccttggct ttggaataag tcagcatacc gttcacaccc catttgcctt ccggttggtc
      481 atagcccgag ccgacgacat agcgcgaggg ttggatggca tcaaacaggt gtgattggat
      541 atcggtgcgg tctgcgcgtt ttttaatatc gcggacacgg acacgattat aggcaaatgt
      601 ggaataccaa ccttcgggca atttatccca tacgccgttc caatcgattt tgcccaaaat
      661 attgatgccg gtaatccgcg cgctttgggc attgaggtaa gccgggtcgc ctttgacttg
      721 ttctttgccg tctttaattt gcgcttcata accccggaca atcaaatcgc ggtaggcatt
      781 gttgaaccaa cttgcctcca agttgccgaa atcgccttta aacacgatgc cggcttcttt
      841 gttgaacgat ttttccggat cgattttgac ggcttttatt ttatcgcccg accgccagcc
      901 gtacatttcc gcaaacgagg gcaggcggaa gccggttgag gtgcggtaag tcaaatccag
      961 ccagtcggca ggtttgagga cgatgccggc gttccaggac agggtgcggt gcgtgccggt
     1021 ggaaacgctg ccgtcgtccg aatgcgtgct gcggtagtcg tagcgcaagc ccgcgccgac
     1081 atccgcccac ctgcccaaac ggacgttgtc ccggaccgcc gcgtaatagc ttttgccgtt
     1141 gatgctgcgc ggcgtgcagt ccgtataagt attgttgcca aagaggcaga tttgccccgt
     1201 aaccacattt cccccgccta tgctgaccca ataggggctg gtgttgctgc cgttggggct
     1261 ggtgttgctg ccgttggggc tggttttttt gccgttgttt tgagggggcg ttttcgacga
     1321 ataggcgcgg ttggcatgtt gataataata atcctgatgg cggaggtcgg aaccaaaacg
     1381 gtcgtaaccg agattcacgc tcaggttgtg gcggattttg gcggtatcga aggatttttt
     1441 gaatgccgcc tgcaagagcc tgtggctttc cccgtaaatc acgcggtcgg atttgtagta
     1501 ggaagacggc ttgtcggcac tcgggcggca atatttgtcc gaaccgtcgg cggaacagtg
     1561 cgtctgctga aaatgattgt ccaaaccgat accctgacgg tcgtaagaga ggcgggcata
     1621 atccgcccaa gtgtctttat cggcattggt atagacatat tccaaaccgt agcggctttt
     1681 ggtgtgcgtc tcgtcgtaaa acacgcccgt accgtattcc gcgcccacct gcgcaccgtt
     1741 ttcactgttg gtaatcagcc cgccgtatcg gtggtcgccc gcgtatttgc cgtagcctct
     1801 tatcaatccg tattttttat tttcatcaaa aaccgccttg gtcaggaatg ccggaaccgt
     1861 catatcgcgc gtgtcgaaag tttgctgcgt gcgttcgagt atgccgccga tgtagtgccg
     1921 cttattctca aaacgaaaac ccgggcggaa cagccacgac cggctttcgt atgaaagcgg
     1981 atcggcaagg aagcggttgg ggcccgtgta gtctcgggtg gaaaccgttt gacgttcgtc
     2041 tttgccgtca acatcttttt tcgctttaca cttttcgtaa cccccgtttt tgcattcttc
     2101 ttcaacgatg aaataggcgt atgtgctcgc gtcatcaacc ggcaccagcc tgttaaagct
     2161 ctgaacgccg cgtccggcgg cttcgtgggc gcggatttcc cccgcgtgcc ggccggtgcg
     2221 gatcagcaaa gcctccgcac cgccgatgcg ccccgccagc gcgatggatt gggtaagccc
     2281 ccggtttttg ccggaatagg cggttttact ctgaatgccc cactgcctgc cttccccgat
     2341 aacatcgtcg gcggttttgg tttgaaatgc gaccgagccc gccaatgcgc cgctgccttg
     2401 ctcgaccgag tttgagccct tgctgatttc gacagccttg acgttctcat actcgatttc
     2461 attgattgcg ccgctgctgc ccgccgtcct cgtcccgccc aatgccgcct gcgcggtgta
     2521 ggactgtatt tgcgccaagc cgtccaccgt caaggcgacg cggtttttgt ccataccgcg
     2581 tatcgagtag cccgagcttg cgccgcgccc ctgttcgacg acggcgatgc cggggtcgta
     2641 acgcgtcagg tctcggatgt cgagtacctg ttctttgctg agggtgtcgg cggttttgac
     2701 caatttgccc aaaccggtta cttcgttatc gcggcgggtt ttctgttttt tggcttttac
     2761 ctgtatggtg tccaactgtt tttcctgtgc ttgtccggct tgcacatttt ctgcataagc
     2821 gggcagcgca gtcattaaag acaggcataa aatattgaat cggaacaaat gttgctgttg
     2881 catagtgttt ccctaccctt cgctttcaga cggaatcgga aggaacgatg ccgtctgaag
     2941 ccttattctc gattgttcgg cagccgtgct tattgcacaa gctcttggcg tttcgcaccg
     3001 aataccacgg tcgcgctgcc tgctgaattt ccattgccgg atgaagtttg cgcatttttc
     3061 gtttgtccat tgcccggata ggcaaaccat ccgcccaact cttcggcttt aggcccgtaa
     3121 aaaccgccct gcaccgcggc acttgtgatg tgcactttat gtgtaccggt gctgctgttt
     3181 tgatccggcg caaagccgcc attaccggtt ttcgccgtac ctttaaagcc gttgccatca
     3241 atcatggcgt caatggtaaa ggttgcctcc gacctgtttt cagcggttaa cttgccggta
     3301 atttttttgt ctttgaaatt cacgtcaaac cttgcccgat taccactttg ctgatcggag
     3361 gcgttgccgg tccagcttgt gccgttggcg gcaatatgcc cgtaccaagt gcctagataa
     3421 acgacgtttt cgtcttttgg aatttctttt tcatcggtgc gctcgccttg aaggaacata
     3481 ctttgtgcgc cctgtgccgt ttggacggct tggctgccgt tgccgcctcc ccccgtgttg
     3541 ccggcggttt tgcgcgtcag caacccgtat ttcagataat tgaggttgga acagcaggct
     3601 tggacttcat aatgggtttt tgttttgcca cttgtgccgc ccgccgcatt tgaaacggtt
     3661 tgcacgccat tcgccgccat acctgtttgg gtgtcttttt tatcactttc cggcgtgtag
     3721 gttgttgtgt aggtaaagtc tgttccaccg tttttacctt tatctgcctg actgctcccg
     3781 ccttcggaat ccttggacag gagcggaatc ataatgccgt cgacaaccag tcgggtagcg
     3841 tcgctgaagt tgtcgagatc tttgattttc ttgccgtcta gtgtcaattc aaccgcatcc
     3901 aaaaccgtgg tcagcctagt gtcttcagac gacatgcctg ccgcacctgc ggtgccagcc
     3961 gccgcagtat tgccatttgc gtttttgtct ttggttttcg cgctgccgac aaccgcaact
     4021 ttttcatcgt cgctcaaaaa gcggaaaccc aattcctcac ccttcgggcc gaaaaagccg
     4081 ccgctcaaag aagacgagtc ggaaacaaag ggatgttgtt tggttccatt cttttgtagt
     4141 ttgtcggtcg ctattgcctt gccattgaag cggttgcctg ttacttgagc ctcaaggctg
     4201 taatattgcg tggtgggttc atccttatca ttgattacca tgttgttttt aattaatttt
     4261 cctttgatgg ttttttgctc aaaatcaacc gttatttccg ttgttaatcc caaacctttg
     4321 tgcttttctt ttttatcatc ctcttttttg actatataat ccaactcccc ggaaaaagca
     4381 ctatatcggt cgccggactt cgtaaaagca ttccctaaat ctgtaaattt ctgattttct
     4441 tgcacatcag ttaaaaaatc ccaagtacct ttgtactcaa cactccctga aacaggaagt
     4501 tttctcgagg gatctatgcc tttataaaaa atataaccgt caggaccgtt tctgacttcg
     4561 attattttac tagatgaatc gtctctttta attgtaatcc caatccgttt ataaaaaaaa
     4621 cccgaccata cgtatttaaa atcgtgatag cccgtaacct tagatatacc ctcaccttgt
     4681 cgactggaat cttgcaatga ttccccccca tttccagata aggcattaat aatatttttt
     4741 tgtttggaag ggtttgtgat atcaccatta tccgtttgtt cccaatcatt ttcagagagt
     4801 ggtatcttat attctttagg attcgcctgc agataccaat tccgccgctt gaagcgcatc
     4861 gcgaaaccgt atccgccttg gtcttttcgg gcttccggtt ttttggaagg aacatcttga
     4921 tactttggcg cgggacgcgg ggcttcggta tcgacagaat caagatcgaa actgccgcct
     4981 ccgcccagac aagcgctcaa caaaaacacg ggcagcacca tagcagcctg attcaccaat
     5041 ggattgttca taacaaaccc gattcaatta aagaatgata aggattatta ttttatttat
     5101 ttttaaaaaa tttgcaaatg cttttttatt tttttagtaa ataaaccaaa ataccgtcat
     5161 tcccgcgctg cttttaaaac cgcctgcaac cgccaaacct gctgcaatcc gctccctccc
     5221 ctgcgcgggg gagggctggg gagagggcac tttccaagtt gcggcaacct ctcccaaatc
     5281 ccttagtctt ctgaaacaca agccttgcgg cttactgccc tctctctaac tctctcccgc
     5341 agggagagag gacggtgcgg ctgttggggt taaggtttat gcaaactaat c
//
LOCUS       DAXDXW010000105         5210 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXDXW010000105 DAXDXW010000000
VERSION     DAXDXW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.106"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            96..917
                     /locus_tag="ACFB64_001968"
     CDS             96..917
                     /locus_tag="ACFB64_001968"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC1210257.1"
                     /translation="MSSPRQVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATVSGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYKDKLFGLTIQPERLQAIRQER
                     RPNSAYARIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(1003..1665)
                     /locus_tag="ACFB64_001969"
     CDS             complement(1003..1665)
                     /locus_tag="ACFB64_001969"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC1210258.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTQLARHGLPEKSMDEIRTQ
                     ASHGAAGLLKLGANITPEHPDYTAWRTEYLEEYDSRYAQDTTLFDGVNELIAELGRRG
                     IKWGIITNKPMRFTDKLVPKLGFAVPPATVVSGDTCGEPKPSIKPMLHACGKIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(1703..2530)
                     /locus_tag="ACFB64_001970"
     CDS             complement(1703..2530)
                     /locus_tag="ACFB64_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC1210259.1"
                     /translation="MKPDIYALPERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAITHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVRVAKEEVYHFRLMRERLRAFGFDYGDFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVEQRGDSATCGVLDIIYRDEVGHVAIGNRWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
     gene            2913..3536
                     /gene="lolA"
                     /locus_tag="ACFB64_001971"
     CDS             2913..3536
                     /gene="lolA"
                     /locus_tag="ACFB64_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC1210260.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTLPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVRATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            3809..4948
                     /locus_tag="ACFB64_001972"
     CDS             3809..4948
                     /locus_tag="ACFB64_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC1210261.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQTPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKMRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPTKELMEKSFIVSPKSAESVKLGVKLWQGLKAGK"
     gene            5043..>5210
                     /locus_tag="ACFB64_001973"
     CDS             5043..>5210
                     /locus_tag="ACFB64_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC1210262.1"
                     /translation="MNLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSKDG
                     SEDICYKYIDKI"
BASE COUNT         1218 a         1409 c         1443 g         1140 t
ORIGIN      
        1 gggtgtaatc cgaggtaggg gcggctgggg tgcttctccc ttgtctgccg ctgctgttat
       61 gatgatattt ttattctgta tttaaggagg gggtgatgag cagtccgcgc caagtgtttt
      121 atatttccga ccgcaccggt ctgactgccg agaatatcgg cgaggctttg ctgaaccagt
      181 ttggcaatct gtcgttcaaa cgccatacgc atccgtttgt cgatacgccg gaaaaggcgc
      241 gcgcggtggt ggagaaggtc aatcggagcc ggcaggaaaa cggtcagcgt ccgattgcgt
      301 ttgtcagtgt ggttgatgac gaaatccgcc ggattattaa aggggcggat gcttttcaga
      361 ttaatttctt tgagactttt ttgggactgt tggagaagga actcaatacc gaagcaacgg
      421 tatccgggca ggggcatcac agtatcggca atacgaagcg ttacgatgcg cgtatggagg
      481 cagtcaattt ttctttgaac catgatgacg gggtcagcga taagaacctt caggaggcgg
      541 atgtgattct gatgggcgtg tcccgttcgg gcaaaacgcc gacctgcctg tatctggcgt
      601 tgcaatacgg tatccgtgcg gcaaactatc cgctgattcc cgatgatttg gaatcggccg
      661 atctgccgcg tatggtcaag ccgtataaag acaaactgtt cgggttgact atccagccgg
      721 aacgtttgca ggccatccgt caggaacgcc gcccgaattc ggcttatgcg cgcatcgaca
      781 cctgccgcag cgaagtggcg gacgcgcaga gtatgttcag acggcatggc attccgtttg
      841 cgaatacgac ggacaagtcg gttgaggaac tggcggtaca catccttcag gcgtgcaagc
      901 tcaaacgcag gttttgacgg gctttgattc ggtttgaagg tggaaatgcc gtctgaaatc
      961 aggtttcaga cggcagtttt atgtttgtgg ggcggatatt tttcagggct gtattttgtc
     1021 cagacattcg agcagatcga gcggcgtgcg gatgtggaaa tccgcctgcc atgagccggt
     1081 atcgtcttca tcggaaatat agccccattc ggcgaggacg gttttcatac cggcgttgcg
     1141 tccggcttgg atgtcgcgtt ccgcgtcgcc gacgtagagg gtgtgttgcg ggtcggcgtg
     1201 gatttttccg caggcgtgca gcatgggttt gatgctgggt ttgggttcgc cgcaggtgtc
     1261 gccgctgacg acggtggcgg gcggaacggc gaatccgagt ttgggaacga gtttgtcggt
     1321 gaagcgcatg ggtttgttgg tgatgatgcc ccatttgatg ccgcgtctgc cgagttcggc
     1381 gatgagttcg ttcacgccgt cgaagagagt ggtgtcttgg gcgtagcggc tgtcgtattc
     1441 ctccagatat tcggtgcgcc atgcggtata gtcggggtgt tcgggcgtga tgttcgcgcc
     1501 gagtttgagc aatcctgccg cgccgtggct ggcttgggtg cggatttcgt ccatgctttt
     1561 ttcgggcagt ccgtggcggg cgagttgggt gttgagtgcg ccgccgaggt ctagggcggt
     1621 gtcggcgagc gtgccgtcga ggtcgaacaa tacggcttgt atcatgtgtt ttcctttttt
     1681 ataaagtgtg ggacgaaagg tttcagacgg catgtttatt ttgtttcaaa ccctgctcga
     1741 aatcttccaa catatccaat tcgaagcggc tgaagcccgc tttttcccgc gcttcgatgt
     1801 tcacatagcc gcggaagata aacatatcgt aacgggcaat cagactgcgg aacagggcga
     1861 caggttccaa accgcgttcg cggcagaggt gttgatacca ccggttgccg atggcgacgt
     1921 gtcccacttc gtcgcggtaa atgatgtcca acacgccgca ggttgccgaa tcgccgcgct
     1981 gctccacctt cgcgcgtatc cccggcgtaa cgtccagtcc gcgcgcttcc aacacgcgcg
     2041 gcaccaacgc catacgcaac aaaggatcgt aggcggtttt gtatgccata tcccataaat
     2101 gattgtgtgc ctcgaaatcg ccgtaatcga agccgaaagc acgcagcctt tcgcgcatca
     2161 ggcggaagtg gtacacctct tccttcgcca cccgcaccca gtcgcggaca aactgaaacg
     2221 gcagcgtgcg gaaacggtat gccgcgtcca aagccagatt gacggcgttg aactcgatat
     2281 gcgtaatcgc gtgcagcata gccgcatagc cttctgccgt gttcattttg cgcggggtca
     2341 gttgcgaggg cgcgaccaaa accggcttgt ccggccgtcc cgcgcggggg aagtccgccg
     2401 gcggcgcgtt tgcctccgcc ccgtccgcat tttgaacggc ggcaaacgcc tcatccgtca
     2461 gccgtccttt ttcatcgggg tcgcccgaaa gcagggcgcg ttccggcaaa gcataaatat
     2521 cgggtttcat ctcaagtccg ccgtgttcgg aaaacgaata ttatagcgtt taaaaaaaac
     2581 aagatgaggc atataatctc cgcgattcgg cattccgcgc ccaaaccgtc aaatatagtg
     2641 gattaacaaa aaccagtacg gcgttgcccc gccttgccgt actatgttgt actgtctgcg
     2701 gcttcgtcgc cttgtcctga tttttgttaa tccactataa cgcggcacac aattgaaagg
     2761 gcagcgtggc gcgcctgctt tttccgagcg gtcaaaaaaa tcagccctcg gaaaacgcgg
     2821 tttgcaaaat gcaaaccgcc cgtaacgctg cccgtatgat tgttttgccg cgccgatact
     2881 ttgcgccaca cccatcccga caaggaaaaa taatgatgaa accgcacaac ctgttccaat
     2941 tcctcgccgt ttgctccctg accgtcgccg tcgcttccgc acaggcgggc gcggtggacg
     3001 cgctcaagca attcaacaac gatgccgacg gtatcagcgg cagctttacc caaaccgtcc
     3061 aaagcaaaaa gaaaacccaa accgcgcacg gcacgttcaa aatcctgcgc ccgggcctct
     3121 tcaaatggga atacactttg ccctacaaac agactattgt cggcgacggt caaaccgttt
     3181 ggctctacga tgttgatttg gcacaagtga ccaagtcgtc ccaagaccag gccatcggcg
     3241 gcagccccgc cgccatcctg tcgaacaaaa ccgccctcga aagcagttac acgctgaaag
     3301 aggacggttc gtccaacggc atcgattatg tgcgggcaac gcccaaacgc aacaacgccg
     3361 gctaccaata catccgcatc ggcttcaaag gcggcaacct cgccgccatg cagcttaaag
     3421 acagcttcgg caaccaaacc tccatcagtt tcggcggttt gaataccaat ccccaactct
     3481 cgcgcggcgc gttcaagttt accccgccca aaggcgtgga cgtgttgagc aactgatgcc
     3541 ggccgccccg atgccgtctg aacgccgccg aggcttcaga cggcattttc acgcaggcgg
     3601 aacaatgtcc cgcattaccg cccgatcggg caacggaacg gcaaatccgt gaaaattaac
     3661 ggttgcgccc cggctgtttt tgccgtttaa tgcaaacctt gctgcaccaa gggccaagaa
     3721 agccgaccgg ccgcccccac agcttccgat gcaggcggcc cgtccgtccc tgcaatgttt
     3781 tttatttttg aacgaaaggt cgaaaaccat gaaaaaaaca ctggtggcgg cggcaatcct
     3841 gagccttgcc ttgactgcgt gcggcggcgg aagcgatacc gccgcccaaa ccccctccgc
     3901 caagcccgaa gccgaacaat cgggcaaact caacatctac aactggtcgg attatgtcga
     3961 tcccgaaacc gtcgccgcct ttgaaaaaga aaccggcatt aagatgcgtt ccgactatta
     4021 cgacagcaac gaaacactgg aggcaaaagt cctgaccggc aaatccggct acgacctgac
     4081 cgcgccgtcc atcgccaacg tcggccggca aatcaaagcg ggcgcgtatc agaaaatcga
     4141 caaggcgcaa atcccccatt acggcaacat cgataaagat ttgctgaaaa tgatggaagc
     4201 cgtcgatccg ggcaacgaat acgccgtccc ctatttctgg ggcatcaaca ccttggcaat
     4261 caacacgcgg caggtgcaaa aggcattggg tacggacaag ctgcccgaaa acgagtggga
     4321 tttggtgttc aaacccgaat acaccgccaa actcaaatcc tgcggcatca gctatttcga
     4381 cagcgcaatc gaacagattc ccttggcgtt gcactatttg ggcaaagacc ccaacagtga
     4441 aaatcccgaa gacatcaaag ccgccgtcga tatgatgaaa gccgtccgag gcgacgtgaa
     4501 acgcttcagc tcttccggct atatcgacga tatggcggcg ggcaacctgt gtgccgccat
     4561 cggttacggc ggcgatttga acattgccaa aacccgtgcc gaagaagccg caaacggcgt
     4621 ggaaatcaaa gtattgaccc cgaaaaccgg cgtaggcgtg tgggtggatt cctttatgat
     4681 tccgcgtgac gcgcaaaacg ttgccaacgc ccaccgctat atcgactaca cgctccggcc
     4741 cgaggtggct gcgaaaaacg gcagcttcgt tacctacgcg cccgccagcc gtcccgcgcg
     4801 cgagctgatg gatgaaaaat acacctccga cgcatcgatt ttcccgacca aagaactgat
     4861 ggaaaaaagt ttcatcgtat cacccaaatc cgcagaatcc gtcaaactgg gcgtgaagct
     4921 gtggcaaggg ctcaaggcgg gcaaataacc ggaatccctg ccgtctgaaa cctttcgggc
     4981 ggcaggaaac ggcgtgtccg cattaagtca ggattaggat tatttagaaa gatgagatgg
     5041 atatgaattt aagtattgtc gttcctattt ataatgtcga aagttatttg gaagcgtgtt
     5101 taaattctat agaacctata ttaagtaatg aaaatgtcga acttattctt gtgaatgacg
     5161 ggtcaaaaga cggaagtgaa gatatatgtt ataaatatat agataaaata
//
LOCUS       DAXDXW010000106         4708 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXDXW010000106 DAXDXW010000000
VERSION     DAXDXW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.107"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..1350
                     /gene="yegQ"
                     /locus_tag="ACFB64_001974"
     CDS             <1..1350
                     /gene="yegQ"
                     /locus_tag="ACFB64_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC1210263.1"
                     /translation="APELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFAKL
                     DVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLIMT
                     VREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELGMEEIAEIRQECPDIEL
                     EVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESEAGDAQLLQG
                     FNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNSKD
                     LRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIHDAVAGRPFDYSLLSEL
                     EGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDENGWATIEVKNRFA
                     VGDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLNP"
     gene            1481..1954
                     /locus_tag="ACFB64_001975"
     CDS             1481..1954
                     /locus_tag="ACFB64_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFC1210264.1"
                     /translation="MLAACQSGRALKNAHTASAGTPAGESCKVIRGDTAGGGKNIIYR
                     CSNGQAAINAATAAGVLESGIPVSFGGGVPVANGRNLVTRQAANGVGKSDSEACEHAL
                     INAARKFQQTAGKLGGRSVTGFHSYFGKQSLQGGQYDCQAGSFHVRAVMRANVVR"
     gene            complement(2163..4536)
                     /locus_tag="ACFB64_001976"
                     /pseudo
     CDS             complement(2163..4536)
                     /locus_tag="ACFB64_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
BASE COUNT         1175 a         1301 c         1139 g         1093 t
ORIGIN      
        1 gcacccgaac tcttattgcc cgccggcgga ttggaaagaa tgcgcgccgc ctacgattac
       61 ggcgcagatg ccgtttacgc cggcagcccg cgttactccc tgcgcgcccg caacaacgaa
      121 tttgccaaac tcgacgtcct cgagcaaggc attaaagaag cgcacgaacg caacaaaaaa
      181 ttctttttga ccgtcaacac cctgccgcac aattccaaac tcaaaacctt cgttgccgac
      241 atggagccgc tgattgccat gaaacccgac gcgctgatta tggcggatcc gggtttgatt
      301 atgaccgtgc gcgaaaaatg gccggaaatg ccgatccatc tgtccgtaca ggcgaacacc
      361 accaactact ggggcgtgaa attttggcag aacattggtg tcgaacgcat catcctgtca
      421 cgcgaattgg gcatggaaga aattgccgaa atccgccaag aatgccccga catcgaactc
      481 gaagtcttca tccacggcgc attgtgcatc gcctactcag gccgctgcct gttgtcgggc
      541 tatttcaacc accgcgatcc caaccaaggc acctgcacca actcctgccg ttgggactac
      601 aaagtccaca acgccacgga aagcgaggca ggcgatgccc agcttctgca aggtttcaac
      661 tttgaaaaag cccaagaaga agccaaccaa aactttgaag gcatcaacgg tcaaaaacgc
      721 catccctacg ccgacaaagt tttcctgatt gaagaatcca accgcccggg cgagatgatg
      781 ccgattatgg aagacgaaca cggcacctac atcatgaact ccaaagatct tcgcggtatc
      841 gaagtcgtcg aaaaactcgc caaaatcggt gtggacagcc tcaaagtcga aggccgtacc
      901 aagtctctct actacgtcgc ccgcgtcgcc cagtcctacc gcaaagcgat tcacgatgcc
      961 gtcgcaggcc gtccgtttga ttacagcctg ttgagcgaac tcgaaggcct tgccaaccga
     1021 ggctacacca gcggcttcct cgaacgccac caaactcagg attatcaaaa ctacctcagc
     1081 ggccattcca ccgccaaaca aagccaatac gtcggacacg ttaccgaaat cgacgaaaat
     1141 ggctgggcga ccatcgaagt taaaaaccgc ttcgccgtcg gcgattcgct cgaaatcatc
     1201 cacccgagcg gcaaccaaac catcaaattg gaacaaatga cccgcaaagg ccaacctgtc
     1261 gatgttgccc cgggcaacgg cattcaggtc aaaatcccca atatgcaggg caaagaaaaa
     1321 gccctcatcg cacgcgtgtt gaacccctga gccacaatgc cgtccgaacc ccgttttcag
     1381 acggcatttt gttgaatctt actgtacaat ggcaatgatt tcccacagca aacaaggaca
     1441 tccgacataa aaaatcctca tactctccgc cgccgccctg atgctggcag cctgccaatc
     1501 cggccgcgcg cttaaaaacg cgcacacggc atccgccggg acaccggccg gagaatcatg
     1561 caaagttatc cgcggagaca ccgcaggagg cggcaaaaac atcatttacc gttgtagcaa
     1621 cggtcaggcg gcaataaatg cagccaccgc cgccggcgta ttggaatcag ggattcccgt
     1681 cagcttcggc ggcggcgtac cggttgcaaa cggcaggaat ctagttacac ggcaagcggc
     1741 aaacggcgtt ggcaaatccg actccgaggc ctgcgaacac gccctaatca atgccgcccg
     1801 caaattccag cagaccgccg gcaaactcgg cgggcgcagt gttaccggct tccacagcta
     1861 ctttggcaag caatcgctgc aaggcggaca atacgactgt caggcaggtt cgttccacgt
     1921 ccgcgcggtc atgcgcgcca atgtcgtccg ttaacatatc ggcaatcaaa aaatgccgtc
     1981 tgaaacattt ttcagacggc atttttaatc ctgcaacatt actccctgcc ctgagttcgg
     2041 atactgtatc aatataaaac cccatcacac agatttacgg taaaaagccg tccgaatgag
     2101 ttcctgaaaa caattcggac agctttaatc cccaacgagg caattaatcc aataatacta
     2161 gattaaaact tccattccag cgatacggcg taattacggc ctgaggcgcg gtagcggtct
     2221 aagcctttgc catctcggtc gaccgcgttg gtggtgctgt agctatacaa accgcgcagg
     2281 gaatcccaag tggtgtattt gcggttgaac acattatata cgcctgcacg caaagtcagg
     2341 tttttagccg gtttgtagaa gccgtacata tcaaacacat aagccgactt gttcagccac
     2401 gggtaatctt ttaccttttt ctgcaaaggc gtaccccggc ccttgttttc ataaacggtg
     2461 tattgcgcgt ctttggcctt tttcgcaccc agataagtca ggcgggagaa cacaccccat
     2521 ttttcgctcg ggcttcgtag tcgacaccgg caatcacttt cggcggctgt gtggacagca
     2581 ggctgttgtc gcccgacagt ttgcttttcg cataacccag cgagccgaac aatttccagc
     2641 cctcaggaac aaaagacgct attttgtcca cattcagacg gcctgtcagc tcaagaccac
     2701 ggattcgggc cttatcgata ttttgcatct gccattccgg tttttcggaa taaggattgc
     2761 tacacatacc gtagtagtaa ttcatctgag tacagccgac atcgccgctg gtggtcagct
     2821 tctgctcttc agacaagaag ttgcggtaat tgttttgata caggttggca tccaaagtac
     2881 ctttttcgct gcggccttgc agagacaggg tgtgggtggt gctgcgctcg gctttcaggt
     2941 tgggattggg cagccaatta cccgaaccgt ggttgtaagt gaaatacact tcggacgcat
     3001 tggggacacg gtagccggaa gtaatgtcgt aaccgacatg ccaagcctga ttcagttgcg
     3061 ccgccaaacc gacaaatccg ctccagcctt tataagtatt ggctgcaggc ggtgttttgt
     3121 cacaagcatg acactcggca ttcaattcct gaggcgtcat tttggtatga tcgtaacgga
     3181 tatctgcacg gctgctgaac acgtcgttcc attggatttg atcagacagt gagaaaccat
     3241 aattagtggt tttcacaggg tgttgaatcg agctggtagt acgggatatc tgtccgctga
     3301 agtaataatc gtcgcggttt aagttttcaa actcacgccg actggcgaaa gttttaaacg
     3361 acaagcgatg ttggccgccc agttgcaacg gttggctgtc catacgcaaa gtaaaacgtt
     3421 tgaatcgggt gtccatgctg cggttgtata tttcgtccaa atccttctga tttatagttg
     3481 cgcgtccagg tggaataatc catcgggaac gagcctttgt tgttaaccgc cgccactttg
     3541 gttttctgat aatcgaagtc cgccttcaaa gacgacaacc aatttgaatc aggcgtccat
     3601 tcgtaaaaga ggttggcatt gcgccgtctg tttacgtcat cggcttcgcg ccaggaagaa
     3661 gcggtcaggt tataagactc ttcaaatcgt gtaattatgc ccctgctggc cgttaaacga
     3721 tgcgccgatg cggtgcttgt cgttgatttg ataagcaatc ttacccaaga agttgtggta
     3781 tttgtgtttg gacggatcag ggataccgcg tgccgaacca cggatattcg ctccgctgcc
     3841 agcaccctct accggatagc cacgctcgcc cgcgctttcg gtctcatgac cgcgacgttg
     3901 cgaatacagc aaagcggcat ccacgcggtc gttgctcaca ccgaaaccga gtgtatttgt
     3961 ccattcgcgg ttgcggctgc tgtaaccgtt tttcatcatc acgccgaatt gcctgtcgtc
     4021 caacagcaaa tcatggcctt gcagggtttg gtaattcacg ccgccaccca atgcgccgct
     4081 accggtattg aaagagtcag cgcccttcgc gatttcgatg ttgcgcacga gttcggggtc
     4141 gatagacagg cgcgagctgt tgaagttgcc ataacgtgca tacagtgagt tttcttccga
     4201 atcaggcagg ctcacgccgt caatgctgac accgacacgg ttgccttcca cgccgcgcac
     4261 agcaaagcct ttttgatggc ggccgctatc gctcaagccg acgtcggtgg agtaacgcac
     4321 caagtctttg ttgtcgcgta tcatttcctg ttgaatgcgg ccgaggttga cacgttccac
     4381 ggttgcaggc gcattaagct ggtctttaac gcgcacttct tttatctctg ctttaacggg
     4441 tgtggtttcg gttgcagctt catccgctgc caagaccgga ttgccgaaaa tactgccgac
     4501 cagcgcggca ataggaagca tgtgtaatgg tttcatatta tcaactcaag atgtaatgga
     4561 ttgttcatcc atcagttaat tcataataaa ttttctgata atcattaata tttaataaga
     4621 cagcaacccg tacaaataaa tctgtgtcgt gtaattaaag gtctctgcaa acatctatgc
     4681 cgaggccttg tttgtttcat tattttta
//
LOCUS       DAXDXW010000107         4516 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXDXW010000107 DAXDXW010000000
VERSION     DAXDXW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.108"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(38..763)
                     /gene="aat"
                     /locus_tag="ACFB64_001977"
     CDS             complement(38..763)
                     /gene="aat"
                     /locus_tag="ACFB64_001977"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC1210265.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(833..1267)
                     /gene="fur"
                     /locus_tag="ACFB64_001978"
     CDS             complement(833..1267)
                     /gene="fur"
                     /locus_tag="ACFB64_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC1210266.1"
                     /translation="MEKFSNIAQLKDSGLKVTGPRLKILDLFEKHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1494..1871
                     /locus_tag="ACFB64_001979"
     CDS             1494..1871
                     /locus_tag="ACFB64_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC1210267.1"
                     /translation="MNKTLILALSALFSLTACSVERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDALQNAAEALRAKQNADKQ"
     gene            1881..2690
                     /gene="dapB"
                     /locus_tag="ACFB64_001980"
     CDS             1881..2690
                     /gene="dapB"
                     /locus_tag="ACFB64_001980"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC1210268.1"
                     /translation="MIPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIRAAAEKTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2804..3073)
                     /locus_tag="ACFB64_001981"
                     /pseudo
     CDS             complement(2804..3073)
                     /locus_tag="ACFB64_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3164..3322)
                     /locus_tag="ACFB64_001982"
     CDS             complement(3164..3322)
                     /locus_tag="ACFB64_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210269.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGSLTD"
     gene            complement(3330..4502)
                     /gene="lpxB"
                     /locus_tag="ACFB64_001983"
     CDS             complement(3330..4502)
                     /gene="lpxB"
                     /locus_tag="ACFB64_001983"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC1210270.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARL
                     TGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRELVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARKTLGADVGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAARFLLPAATEATKRRLAEVLQRPEFAGLALTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFGALHLLLKKDTADLAARAVLEEAGC"
BASE COUNT         1125 a         1457 c          996 g          938 t
ORIGIN      
        1 ccactatatt tgaacctgac cgaagcataa gccgccatca aagccccttg cacgccactt
       61 cgcgccgccc gatttcctct ttcaacggca cggcgttcaa catccgcaga cgttcggcaa
      121 aatccgcaaa cggcagcagc tccgaaccga aacggcgcat atgttccgta tcctgctggc
      181 agtctatcag ttccacgccc aaatccgcca aaaacggcac ggcgcaggca aacgcgattt
      241 tcgacgcatc cggttgcaac gcaaacatcg attcgccata aaacaccctg ccgatctgaa
      301 cgccgtaaaa gccgcccgcc aacctcgttt taccgttttc atcgggataa tggcactcga
      361 aagaatgcgc gtgtcccatt tcgtgcagct tcaaatatgc cgtctgaaac tcgggcgcaa
      421 tccaagtccc atcctgattc gggcgcgccg ctgccgcaca atgcgcgaca acttccgcaa
      481 aacagccgtt gaccgcaacc cgatagctgc cgttacgcag cgttttcgcc agcgagcgcg
      541 gaacgtgcag acgttcggga acaattaccg cacgcggccc gaccgcatac caaaaaaacc
      601 aaccgtcccg cgaaaaccaa ggaaacacgc cgttccgata cgcctcgagc agccgccccg
      661 cgtccaaatc cctgctcacg ccgaccagcc cgtcgcaccg ggccaaagca taggcaggat
      721 cgggaaaggc ataattgtca ggggcaagca gcggaatacg catggcagtt ctccaaaaac
      781 acagacggca tccaaaatca tgaatgccgt ctgaacaaca aaccgtccgg atttaacgtt
      841 tgcccttggc ctgacagtcg ctgcacacgc cgtacatata aagcgcgtga tcgacgatgc
      901 ggtagccgtt ttcctcggcg attttgtctt gcagggcttc gatttcggga ttgtggaatt
      961 ccgttacctc gccgcacttc acgcagacga tatggtcgtg gtggtcgccc ttgtccaact
     1021 cataaaccgc cttgcccgtt tcaaaatggt ggcgttgcag aatgcccgcc tgctcgaact
     1081 gcgtcagcac gcggtaaatc gtcgccacac cgatttccac gccctcttcc agcagaatgc
     1141 ggtacacatc ttccgcactc aaatgctctt ccgcgtgctt ctcgaacaaa tccaaaatct
     1201 tcaaacgcgg gccggtaacc ttcagaccgc tgtctttcag ttgcgcaatg ttgctgaatt
     1261 tttccataat attcaatatc cctgtaaaat aatagacgct ataatacgca atttcagcct
     1321 gcttgcccac tatcacacca tagcagttcg caatagcaaa accgcaacgg cggacgggca
     1381 gtcggacaca gacagggtaa aataccgctt aagcgtatga ttatcgttcg cttttataaa
     1441 atattcaagc agtgctacac tagacatccc gatttgcaca gaaaggttct cccgtgaaca
     1501 aaaccctcat cctcgccctt tccgccctgt tcagcctgac cgcgtgcagc gtcgaacgcg
     1561 tctcgctgtt tccctcctac aaactcaaaa tcatccaagg caacgaactc gaaccgcgcg
     1621 ccgttgccgc cctgcgcccc ggcatgacca aagaccaagt cctgctcctg ctcggcagcc
     1681 ccatactgcg cgacgctttc cataccgacc gctgggacta taccttcaac acctcccgca
     1741 acggcatcat caaagaacgc agcaacctga ccgtctattt tgaaaacggc gtactcgtcc
     1801 gcaccgaagg cgacgccctc caaaatgccg ccgaagccct ccgcgcgaaa caaaacgcag
     1861 acaaacaata aggaacacac atgataccgc tcaaaatcgc catcgccggc gcaaacggcc
     1921 gcatgggacg cgtcctcgtc gaagccgtca acaaccatcc cgacaccgtc ctttccggcg
     1981 cgctcgaaca ctcaggctca gaagccctcg ggctggacgc gggctacgcc gtcggactca
     2041 aaaccggcat cgccatttca gacgacgttg acgccgttct cgcccaaagc gacgtactca
     2101 tcgacttcac ccgccccgag ccgaccctca agcacctgca aaaatgcgtt gaaaaacaag
     2161 tcaacatcat catcggcaca acaggcttcg acgatgcggg caaagccgcc atccgcgccg
     2221 ccgccgaaaa aacaggcatc gttttcgccg ccaacttcag cgtcggcgtc aacctcacct
     2281 tccacatcct cgacaccgtc gcccgcgtcc tcaacgaagg ctacgacatc gaaatcatcg
     2341 aaggccacca ccgccacaaa gtcgatgccc ccagcggcac agcattacga atgggcgaag
     2401 tcatcgccgg cgcgctcggc cgcgacctca aacaatgcgc cgtttacggc cgcgaaggcc
     2461 acactggtcc gcgcgatcct tccaccatcg gctttgccac cgtccgcgca ggcgacatag
     2521 tcggcgacca caccgccctc ttcgccaccg acggcgaacg cgtggaaatc acccacaaag
     2581 ccggcagccg catgaccttt gccgccggtg ccgtccgcgc cgcagtttgg gtaaacggca
     2641 aaacgggttt gtacgatatg caggacgtac tcgggctgaa caaccgttaa cccccataca
     2701 aaatgccgtc tgaaaagata ttgttcagac ggcattttgc cgacaggctt cgtatcggca
     2761 tatcaataaa gttgcttgtg cagcatgaca agctacggct cgtttaattg aaactctcct
     2821 gatctaaaaa ttccaactct atttcccggc aaactatatc tatgataaaa ctggtcggaa
     2881 gattatacct acgccttatt aatgccagtg cacctccata atccaagatt tcatcatcct
     2941 gctttaattc tccaagctcc aaggtattat cgatatcttt atcttggttc tccgtagcgt
     3001 atccatagat aaatttcaaa tagctgaact gcttgaattt aatttttacc cagtctgtgt
     3061 aatttggtgt aggacacaat tgcctaaaat agaaatttac gaagaacaaa tgaaacaagg
     3121 tacgtaagcg tattagtata aactataatc atatataata aaattaatca gttaacgaac
     3181 cattatattt taaccttaat cccattcggg ccagttctgt ttcatttgga attcctctta
     3241 aatacttatt gtttgatgtg ccattaatca caatttctcc cccttttttt gtaatacgtg
     3301 ctgcttcggg aaaataatcc attaaccgct caacatcccg cctcttccaa aaccgcacgc
     3361 gcggccaaat ccgccgtatc ttttttcaac agcaggtgca acgccccgaa atcctgttgc
     3421 agcgcggcaa ccttatcggg gtgttcgtac cagtccgcca atgccgccgc cagtttttcc
     3481 ggttttgctt cagattgcaa taattccggc acggcctcct tacccaacag gatattcggc
     3541 aggccgacat gcggcacttt gattttgcgt ttcacataag cataggtcag cggcgaaatc
     3601 ttgtagctga tgaccatcgg acgcttgcac aacgccacct ccaaagtcgc cgtaccgctc
     3661 gttaccagca ccgcatccgc cgccctgcac accgtttcag actgtctgtc ggttaccgtc
     3721 agcgccaatc cggcaaactc cggccgctgc aaaacttccg ccaaacgccg cttcgtcgcc
     3781 tccgttgcgg caggcagcag gaagcgggcg gcgggatagc gttccaacaa caataatgcc
     3841 gtctgaaaaa acaccggcgc catatagtcg atttcgctga cgcggctgcc gggcagcagg
     3901 gcgaatacgg ggatgccgac atccgcgccc aaagttttcc gcgccgtttc acggtcgtct
     3961 tccaagggca taagctgtgc catcggatga ccgacaaact ccgcacgtcc gcccgcatcg
     4021 agataaagct gcggctccat cgggaacagg cacagcacgc ggttgacctg atgcacgatt
     4081 ttgcccacgc gttcgcgccg ccacgcccaa accgacggac tgacataatg cagggtcgga
     4141 atgcccgccc gtttcagctt ttccgccaca cccagattaa aatcgggcgc atcgataccg
     4201 acaaagacat caggtttcaa cgacagcaaa tcccgtacca gctccctgcg tatccgtaaa
     4261 atttccggca gccgcctgac cacttcgaca aagccgcgca ccgccagccg ctcctgatca
     4321 taaaggctct cgaaaccttc cgccttcatc agttccccgc cgatgccggt taaccgtgcc
     4381 tgcgggcaac gctttcggat ggcgcgtatc aggtgcgccc ccaacaggtc gcccgacgct
     4441 tcgccgacac tgacggcaat caaagggctt tttttatcaa ccatattcgt ctgtccccac
     4501 atatagtgga ttaaca
//
LOCUS       DAXDXW010000108         4386 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXDXW010000108 DAXDXW010000000
VERSION     DAXDXW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.109"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..1212)
                     /locus_tag="ACFB64_001984"
     CDS             complement(<1..1212)
                     /locus_tag="ACFB64_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210271.1"
                     /translation="MKQKKTVQCILLGFAAASMHAQGAAAANSGTIEKTDKYTLVLAK
                     QGQENNYTLNGGTEVKPLNSLIIAANGGTNNITIKGKLADGPADAPPTIDNNSIERNI
                     NKNGYTYAWQNWSGAVMLVDQSYEGENKVTFENVTIAAHNAPAGILSDDRHKSSSLAP
                     AMLAFKGRNTINMDADSNANSSNEGILLLNNGEKMGEYRLVSEEGSTLNINIKSGKDK
                     GQGITANHYGNSDINFNKASPNITTMEFKGGVNIKIDRNGQEEAESNGFGFYSSRKLG
                     NKKQIPEGSKMEAIFRGNVDIVATPVYDEQGRPKSIGSAFAIDGKYSKVEVVGGEGKV
                     VKIKGDIFAYNGGSVSVNLANKDSYFEGEAHIGKRSFAKGKDMFALTVDADGYELTPD
                     TKSIEKKKKELN"
     gene            1409..1484
                     /locus_tag="ACFB64_001985"
     tRNA            1409..1484
                     /locus_tag="ACFB64_001985"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1443..1445,aa:Glu,seq:ttc)
     gene            complement(1700..1852)
                     /locus_tag="ACFB64_001986"
     CDS             complement(1700..1852)
                     /locus_tag="ACFB64_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210272.1"
                     /translation="MPSENRSAGFVVHYYVVLQDKAQGKAAGCIVATDNEKRGRCRLN
                     RVLRRV"
     gene            1912..2718
                     /gene="thiD"
                     /locus_tag="ACFB64_001987"
     CDS             1912..2718
                     /gene="thiD"
                     /locus_tag="ACFB64_001987"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC1210273.1"
                     /translation="MKGSFVQTLAIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDILTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVIIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            complement(2776..3645)
                     /gene="tehB"
                     /locus_tag="ACFB64_001988"
     CDS             complement(2776..3645)
                     /gene="tehB"
                     /locus_tag="ACFB64_001988"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFC1210274.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFDVTAADCNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPG"
     gene            3758..4195
                     /gene="rnhA"
                     /locus_tag="ACFB64_001989"
     CDS             3758..4195
                     /gene="rnhA"
                     /locus_tag="ACFB64_001989"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC1210275.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSREKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWQELDALVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
BASE COUNT         1009 a         1235 c         1039 g         1103 t
ORIGIN      
        1 attcagctct ttctttttct tttcaatcga ctttgtatcg ggggttaatt catatccgtc
       61 cgcatccacg gttaaggcaa acatatcttt ccctttggca aagctccttt tcccgatatg
      121 ggcttcccct tcaaaataag agtctttgtt ggcaaggttc acgctcacgc tgccgccgtt
      181 gtaggcgaaa atatcgcctt tgattttgac aaccttgcct tccccgccga caacctcgac
      241 tttgctgtat ttcccgtcaa tcgcaaacgc gctgcctatg cttttgggcc tgccttgctc
      301 gtcataaacc ggcgttgcaa cgatatcgac gttcccacgg aaaatcgctt ccatcttaga
      361 accttccgga atctgcttct tattgcctaa tttgcggctt gaatagaagc caaaaccgtt
      421 gctttccgcc tcttcctgtc cgttcctatc gattttaatg tttacaccgc ccttgaactc
      481 cattgtggtg atgtttggag aagccttgtt gaagttgatg tctgagttac catagtggtt
      541 ggcggtaatg ccctgtcctt tatcttttcc tgatttgatg ttgatattca gcgtcgagcc
      601 ttcttcggaa acaagacgat attctcccat tttttcacca ttattaagca ataggatacc
      661 ttcgttacta gagttggcat ttgagtccgc gtccatattg atggtattgc gccctttaaa
      721 ggcaagcatc gccggcgcca agctactgga tttatgccta tcatcagaca aaatgccggc
      781 aggcgcgtta tgggcggcta tggttacgtt ttcaaaagta accttatttt ctccctcata
      841 gctttgatcc accaacatca ccgcgccgct ccaattctgc catgcgtacg tgtaaccgtt
      901 tttatttata tttctttcaa tcgagttatt gtcaatcgtc ggcggggcat cggcaggacc
      961 gtctgccaat ttgcctttta tcgtaatgtt gttcgtaccg ccgttggcgg caatgatgag
     1021 ggaatttaag ggtttgactt cggttccgcc attaagggta tagttgttct cttgcccttg
     1081 ttttgcgagg acgagggtgt atttgtctgt cttctctatc gttccactgt ttgcagcggc
     1141 cgccccttgc gcgtgcatag aagcggccgc gaagccaagt aagatgcact gaactgtctt
     1201 cttttgtttc atcggttttt ccattttgaa ggtatgtgat ttttatcatt atatgcttaa
     1261 atcattgaca cgcccccaaa aaaaatgaat tgcttgatat tccaatgttt tttatcttgt
     1321 gatacggtca gacaaacgcc ccctgacatt cgtttagacg gcatcgtatt gctaaatttc
     1381 tataagtatg tataatgtcc gtttccacgc gcccatcgtc tagaggccta ggacactgcc
     1441 ctttcacggc ggcaaccggg gttcgaatcc ccgtgggcgt gccaattcaa aaacctgctt
     1501 gtttcaagca ggttttttat tatgaatcgt tattcccgca atttttcgtc attgccgtga
     1561 aaacgggaat ctagaacctc aaactttcag ataatctttg aatattgctg ttgttctaag
     1621 gtccggattc ccgcctgcgc gggaatgacg gaattttagg tttctgtttt tgcgggaatg
     1681 acgaatgaag cgtgccggtt tatactcgcc gcaacacgcg gttcagacgg catcgccctc
     1741 ttttttcatt atcagttgct acgatgcacc ccgctgcctt gccctgtgcc ttgtcctgca
     1801 atacgacata ataatgcacc acaaaccccg cgctgcggtt ttcagacggc attgcctgct
     1861 tttttacagg cagtagccct ttttatcgga cgcaatatta aggaggaaca aatgaaaggc
     1921 tcttttgtgc agacgcttgc catcgccggt tcggattcgg gcggcggtgc gggcattcag
     1981 gcggatttga aaacgtttca gatgcgcggc gtgttcggaa cgtgtgtcat caccgccgtt
     2041 accgcgcaaa ataccttggg cgtgtcggcg gttcatctcg tcccgaccga aaccatcacc
     2101 gcacaaatcc aagcaatcag ggaagacttt gacatccgcg cctacaaaat cggtatgctc
     2161 ggcacggcgg aaatcatcga atgcgttgcc gacaagctga aacactgcag ctttggcagg
     2221 cgcgtactcg accctgtgat gattgccaaa ggcggtgcgc cgctgttgca ggattccgcc
     2281 gttgcggcac tgacgcgcct gctgctgccg gacacggaca tcctgacccc caacctgccc
     2341 gaggcggaag ccttgacggg cgtgcatatc gaaaaccgta aagatgcgga acgtgcggca
     2401 aaaatcctgc ttgattacgg tgtcaaaaat gtcattatca aaggcggaca tttgaacggc
     2461 agcacaagcg gacgctgcac ggattggctg tttacgcaaa acgaaacgct ggaattggac
     2521 agcccgcgct ttccgaccgc ccacacgcac ggcacgggct gcacgttttc cgcctgcatt
     2581 accgccgagt tggcaaaagg cttagacgtt tgcaaagccg tacagactgc caaggcctac
     2641 atcacggcgg caatctcaaa ccctttggaa atcggcgcgg gacacgggcc ggtcaatcat
     2701 tgggcgtatc gggactagac gtaaaaatgc cgtctgaacg ccttttcggg ttcagacggc
     2761 atcgtccggc gcggcttatc ctggtttttt cgccagcata gttacaaatt taaaccgtat
     2821 cgggttgccg ttttcgtctt tggcgtgcat cgcgcccaat tcttctttat attcgaccag
     2881 ctcccaatcc cgataataat ccttcagctc gccctcttta aatttgaagg ggaacggcat
     2941 cgggcagggg aaatccgccg tatccattgc cgatacgatt aagttgtacc cgcccgccgc
     3001 cgtatgcgcc tgcatatcgg caatcacgtc gggcacgcgc tgcggcatca ggaacatcag
     3061 caccaccgtt gccacaatat aatcaaactc gccctgaagg gcggcggcgt tcaaatcata
     3121 ttccagcgtg cggacgttca aaccctccgc ctctgccagt tccgccacgt ttgccaacgc
     3181 ggcgggattg caatcggcag cggttacatc aaaccccttc aaacccagga acagcgcgtt
     3241 gcgcccctgt ccgcagccca tatccaacgc cctgcccgcc ggtacggtat cctgtgccgc
     3301 cgcgaccgca gaatgcgtgg cactcatccc gtattttttg tgaaaatagt ctgccgccgc
     3361 gcaatacagc gacaaacgga tttcggcatc gtccgttttc ggtttgaccg aaaacacctg
     3421 ctgcggcgca aacacgcaat cgccgctttc cgccgaccaa acctctgccg ccccgtccgg
     3481 tgcacggact tcgacatcgc cctgcaacac attcaggcag acccattccc cttcctcgga
     3541 cgaataaccc gacaacaaaa cttccggcag gttttccact ttccatacag gcatttgtcc
     3601 gaaacaaaac aactcgccac tttgacccac tatccgctcc ttcatattca aaaataaagt
     3661 tgcacattat atgcctattt caatccgccg caatctttca gacggcacga cgcacaaacc
     3721 gcttataatc acgccggaca ccacacaaag gcacgatatg gacacacccg tttacctcta
     3781 cacggacggc gcgtgcaaag gcaatcccgg cgcgggcggc tggggcgtat taatgcgcta
     3841 cggcagccgc gaaaaagaac ttttcggcgg cgaagcgcaa accaccaaca accgcatgga
     3901 gctgaccgcc gttatcgaag ggctgaaatc gctcaaacgc cgctgcaccg tcatcatctg
     3961 caccgactcg caatacgtca aaaacggcat ggaaaactgg atacacggtt ggaagcgcaa
     4021 cggctggaaa accgccgcca aacagcccgt caaaaacgac gacttgtggc aagaactcga
     4081 cgctctggtc gggcagcatc aagtcagctg gacttgggtt aaaggacacg cgggacacgc
     4141 ggaaaacgaa cgcgccgacg atttggcaaa ccgtggcgcg gcgcagtttt cttgattgcc
     4201 gcaccgacaa aaatgccgtc tgaaaccgct aatgggcttc agacggcatc gtcctccacc
     4261 gtcattcccg cgcaggcggg aatccagaac gtcgggcaac ggcaatattc aaaagccgtc
     4321 tgaaaattta aaagttctag attcccgttt tcacgggaat gacgaaaagt tgcggacagc
     4381 agagga
//
LOCUS       DAXDXW010000109         4365 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXDXW010000109 DAXDXW010000000
VERSION     DAXDXW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.110"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(106..1137)
                     /gene="gap"
                     /locus_tag="ACFB64_001990"
     CDS             complement(106..1137)
                     /gene="gap"
                     /locus_tag="ACFB64_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC1210276.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAVSEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(1336..2175)
                     /locus_tag="ACFB64_001991"
     CDS             complement(1336..2175)
                     /locus_tag="ACFB64_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC1210277.1"
                     /translation="MTATAADLDRLLPQTQCRECGYEGCLPYARAMLRGEAHNLCAPG
                     GATVVRDLAALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVITDECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSDDSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPEKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRQGYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            2321..4297
                     /locus_tag="ACFB64_001992"
     CDS             2321..4297
                     /locus_tag="ACFB64_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC1210278.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPELEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVEAMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMHPNMPVYARATN
                     DDYVKTFTDMGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKKEIISFENWSLQGI"
BASE COUNT          774 a         1022 c         1472 g         1097 t
ORIGIN      
        1 tggaaatgac gacagccggg taaaatcgcg gtcaaaaatg aaaagtgccg tctgaaactt
       61 gaaaacatcg ggtttcagac ggcattttgt ttgacggttt gttgcttatt tgagcgggcg
      121 cacttcaagt ccgaacatac ggcgtgcggt gttcagcatt tggcagctga agccccattc
      181 gttgtcatac cacgcgaaca ctttgaccat gttgccgtcg acgactttgg tcagcgttgc
      241 gtcgaagtgg ctggcttcgg tggtgtggtt gaagtccatg gaaaccaatg gcagggtgtt
      301 gtagcccaaa acgcctttga gcgcgcctgc ttccgagacg gctttcatca gtgcgttgat
      361 ttcttcgact gtggtgtcgc gcgcggcttg gaagctcaaa tctaccaagg atacgttgac
      421 ggtcggcacg cggatggcaa gcccgtcgag tctgcctttc aattcgggca agaccaaacc
      481 gacggctttt gccgcgccgg ttttggtcgg aatcatgttt tccacgccgc tgcgggcgcg
      541 gcgcaggtct ttgtggcgca cgtcggtaac ggtttggtcg ttggtcagtg cgtggatggt
      601 ggtcattgcg cctttgacga tgccgacgct ttcgctcaac actttggcaa ccggcgagag
      661 gcagttggtg gtgcaggaag cgttggaaac gacggtcatg tcggcggtca ggacgctgtc
      721 gttcacgccg tacacgacgg ttgcatcgac atcgtcgccg cccggtgcgg aaatgaggac
      781 ttttttcgcg ccgctttcga ggtggatttt ggctttttct ttgctggtga acgcgccggt
      841 gcattccatg accaaatcga caccgagttc tttccacggc agttcggcag ggttgcgggt
      901 cgagaagaag gggattttgt cgccgttgac gatgaggttg ccgccgtcgt gggatacgtc
      961 ggcttcaaag cgtccgtgta cggtgtcgaa tttggtcaga tgggcgttgg tttcaaggct
     1021 gccgctggcg ttgacggcga cgatttggag ttggtcttga atctgatagt cgtagatggc
     1081 gcgcaaaacc tggcggccga tgcgtccgta gccgttgatg gcgactttga tgcccatggt
     1141 ttgttccttt gttgagggtt gggtagattt tcggggcgga ttatagcaaa tttgtagtgg
     1201 cgtgtaatta atattttatt gaaaacggca cggccggaag ggtgggcggt aagatacgga
     1261 cggcacgggt acggcggacg gagagcttga taaaatgccg tctgaagcgg cttcagacgg
     1321 catgtcgggt aagggtcagg aggcggtatt ctgtgcggct tcctgtttgg ctttgtattg
     1381 tttgaggtat tcgagggcgg cggctttttc gctgtcgctg ccgtatttca tatcgcgttg
     1441 ggcgcggcgc aattcggcgc gttcgcgggc ttcggctatc tgttttgcct gatagccttg
     1501 gcggttgtcg gcggcggcga ggcgatcctg ttgggtttgc gcttttgcca tggctttggc
     1561 gatgaggtcg gcggggttga acgccggttt ttccggtgtg tcgggcgttt gcggacgcgc
     1621 gttgcggacg gcggcttcgc gttcggcaag catggccttg cgttcgtcgg cttcgcgctg
     1681 tttgcgttcg ttgcgtttga ggtagcgcgc acgcgcgtgt tcggcggcgg caaaacggct
     1741 gtcgtcggac aggctgaaac ggcgcgcgcg cggcaggacg gtgtcggcaa cgggctgcat
     1801 atggatgcag tccacggggc agggggcgac gcagagtccg cagccggtgc attcgtcggt
     1861 gatgacggtg tgcataagtt tgcccgcgcc cataatggca tcggcagggc aggcgcggat
     1921 gcaggcggtg cagccgatac aggcggtttc gtctatccgg gcgagtgctt tggcttgggt
     1981 tttggcaggt gcgacaaggg gtttgccgag cagggcggca aggtcgcgga cgacggttgc
     2041 gccgcccggg gcgcagaggt tgtgcgcctc gccccgcagc atcgcgcggg cgtagggcag
     2101 gcagccttca tagccgcatt cgcggcattg ggtttggggg aggagtcggt ctaaatcggc
     2161 ggcggtggcg gtcattgtgc ggtggtgcgg gtcaaaaggg cgtattttat cagaattgta
     2221 tgccgcgccc gtttcggatg gtgcgcggtg ttttgttata atacggcggc gtatgccgtt
     2281 tcagacggca tttttctgta ttttcctgtt cggacggtcc atgaacgaat tttcgcttgc
     2341 ccctattgtg gttgttctgc tggtgtcggt cattacggtg atcctgtgcc gcaagttcaa
     2401 cattccctcc atgctgggct acctgctggt gggctttttg gcggggcccg gtatgctcag
     2461 cctgattccg aaaagccatg cgacggatta tttgggcgaa atcgggattg tgttcctgat
     2521 gttcagcatc ggtttggagt tttcattgcc caagctgagg gcgatgaggc ggctggtgtt
     2581 cggtctgggc ggtttgcagg tcggcgttac gatgctgtcg gtaatgggca tactgatgct
     2641 gacgggcgtg ccgttcaatt gggcgtttgc cgtatcgggc gcgttggcga tgtcgtccac
     2701 ggcgattgtg agccggattt tgtcggaaaa gacggagttg gggcagccgc acggtcagat
     2761 ggcgatgggc gtgctgctga tgcaggacat cgccgtcgtg ccgctgatga ttctgattcc
     2821 cgcgctggct ggcggagggg acggaaatat ttgggcggcc ttgggtttgg cgtttgcaaa
     2881 aatgctgctg acgctggggc tgctgttttt cgtcggcagc aaaattatgt cgcgatggtt
     2941 caggatggtg gcaaaacgca aatcgtccga actctttatg atcaatgtgc tgctggtaac
     3001 cttgggtgtg gcttatctga ctgagctgga aggtttgtct atggcgttgg gcgcattcgt
     3061 tgccggcatg ttgctttcgg aaacggaata ccgtttccaa gtcgaagacg acattcgccc
     3121 gttccgcgat attttgctcg gcttcttctt tatcacggtc ggcatgaagc tggacattca
     3181 ggcattgatc ggcggctggc ggcaggtatt gatgctgttg gcaatgctgc tggtgttgaa
     3241 ggcattggtt gtgtttgcca ttgcattcaa aatgaagcat tcggtcggcg acagcctcaa
     3301 aacggctttg tatctcgcgc aaggcggcga gttcggcttc gtgatgctgg ccattgccgg
     3361 gcagcttgat atggtttcgc cagaattgga acaggcggcg acggcggcgg ttctgctgtc
     3421 gatgattatc gcgcccttcc tcttgggcgg cagcgatgcg ctggtcgggc gcttggtcaa
     3481 gtcaagctgg gacatgaagt cgctcgatct gcacagcatg ttggtggaag cgatgagcaa
     3541 gtctgatcat gtgctgattg tcggcttcgg caggggcggg cagacggtcg gacgcgtcct
     3601 cgcccaagag gatattccgt atttcgcgct cgacttggac atcgcacgcg tgcaggtagc
     3661 cagaagtgca ggcgagccgg tgtcgttcgg cgatgcgaaa cgcagggaag tattggaagc
     3721 cgccggtctg ggacgggcga aaatggtggt ggttacgctc aacaatatgc acgaaacgca
     3781 acacgtttta gacaatgtgc tgtccatgca tcccaatatg cccgtatatg cgcgcgccac
     3841 caatgacgat tatgtgaaaa cgtttaccga tatgggtgcg gaagaagccg tgtcggacac
     3901 caaagaaacc ggactcgtgc tggcaggcta tgcgatgttg ggtaacggcg cgtcgtaccg
     3961 gcacgtctat cagacgatgg caaatatccg ccacagccgt tatgccgcgt tggagggact
     4021 gtttgtcggt agtgatgatg aggcaggatt cggcgaaaat ggcgaaaccg tccgccacgc
     4081 ctttcctttg gctgcagaag catacgccgt cggcaaaaca gtcggcacgc ttccgatggc
     4141 ggcttacggc atcaaactct tgttcgtccg ccgccgcacc ggccggattg aaaacccgga
     4201 tgcctcgttt acattggaag gcggtgacgt gttggtggtc gcaggcaaaa aaaaagaaat
     4261 tatctctttt gaaaactgga gcttgcaggg aatataaatg aaatgccgaa ataaggcttg
     4321 cgccattccc ggttatttgg tctaatagcg ttttcgcaaa tcgca
//
LOCUS       DAXDXW010000110         4207 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXDXW010000110 DAXDXW010000000
VERSION     DAXDXW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.111"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..228
                     /locus_tag="ACFB64_001993"
     CDS             <1..228
                     /locus_tag="ACFB64_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFC1210279.1"
                     /translation="KKTVKFQFPPEVVRVLAGMGNNLNQIAKALNTAAKVGTLGNVEA
                     LKATTELAALERSLNSLRDFLAKEKNGWQSQ"
     gene            213..842
                     /locus_tag="ACFB64_001994"
     CDS             213..842
                     /locus_tag="ACFB64_001994"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFC1210280.1"
                     /translation="MAVPMIVQFFNRGKGGGSGPIDYLLGKDRDREEARLLRGDPEET
                     AALINSSDYAKKYTAGCLSFEESNIPAEQKHALMDSFEECIFAGLDKDQYNCLWVEHR
                     DKGRLELNFVIPNIELLSGKRLQPYYYAADRGRVDAWRTMQNLTHGYSDPDDPAKRQS
                     MTQAKDLPRNTQEAAQSITEHHRRLRSPSPIRQAKKPRRRAGNAGKGRF"
     gene            926..1567
                     /locus_tag="ACFB64_001995"
     CDS             926..1567
                     /locus_tag="ACFB64_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFC1210281.1"
                     /translation="MSKISDLAKTFEQTSRTEAASIEQQTKADLETLRKNINEALKQS
                     EQKITADINARQLRMSKAVFKPYLWSLLGISAAGLIVIAGLFIAIWSVKNELDDLKQQ
                     RAEAERTLDLLETKTKGLTLENCPVENSKATRVCVATEKRMLDALAELESNHAAIEQR
                     MMKALTHLGERLAELEQENTSLAQQLASLAAELERQSEIRQRQSEILNQPAKR"
     gene            1614..1868
                     /locus_tag="ACFB64_001996"
     CDS             1614..1868
                     /locus_tag="ACFB64_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cryptic plasmid protein A"
                     /protein_id="HFC1210282.1"
                     /translation="MITENERDRRTAAWLIETYGAEAVAEAETRIAGARKPYPSNIAK
                     VLGASLPEALKRTENAAARQKLAGLRRMLDGKAVKTSQDL"
     gene            complement(1846..2124)
                     /locus_tag="ACFB64_001997"
     CDS             complement(1846..2124)
                     /locus_tag="ACFB64_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease VapD"
                     /protein_id="HFC1210283.1"
                     /translation="MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSL
                     YTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRIEQWSDFTSLVKS"
     gene            complement(2124..2309)
                     /locus_tag="ACFB64_001998"
     CDS             complement(2124..2309)
                     /locus_tag="ACFB64_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5397 family protein"
                     /protein_id="HFC1210284.1"
                     /translation="MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESG
                     EHTQYKLSHIVQDPKAA"
     gene            complement(2312..2557)
                     /locus_tag="ACFB64_001999"
     CDS             complement(2312..2557)
                     /locus_tag="ACFB64_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210285.1"
                     /translation="MTRYAINRDALYSAFKDFLYSEINANPALKSATVDDLADIVLAK
                     KWRIFLPDGIKRTTAEKAAQRVLYMTNQPENQTQQEN"
     gene            complement(2554..2796)
                     /locus_tag="ACFB64_002000"
     CDS             complement(2554..2796)
                     /locus_tag="ACFB64_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFC1210286.1"
                     /translation="MAYPKLKTTKRDVTAKELAKRFGCSTRTVFRAWSQSRADYLAEN
                     SISRDKPWEHFGISRATWYRRGKPMPSETDNQSETA"
     gene            complement(2800..3669)
                     /locus_tag="ACFB64_002001"
     CDS             complement(2800..3669)
                     /locus_tag="ACFB64_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFC1210287.1"
                     /translation="MSQLYTQPDLFLQERIPHKPYCKDFKEAPMLVRSYAAAIKRRYI
                     QVNPPHLRVFMLFDLDYEGAGLAWEDNNLPMPAWAAINRENGGAHLAYALSAPVLTAE
                     YGGRQKALRYLAALEAAYKAKLRGDVGFVSLITKNPEHPHWLTLRGVPDAIRGYDLEY
                     LADFVDLDKFKPYIGRSNVEAVGLSRNCTVFNLVSRWAHKNVLAFKQQGYTVQGWLKE
                     VHYQCMRVNGDFPVPMWEKEVKCISKSIANWVWYKFDIAASNRRFSELQAHRNSLRKT
                     TINAGRTKIITEL"
     gene            complement(3752..4150)
                     /locus_tag="ACFB64_002002"
     CDS             complement(3752..4150)
                     /locus_tag="ACFB64_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210288.1"
                     /translation="MGLLSGQSLKLGRADSDDELLCSLFGGTQTCQKGKIPLFLTAFC
                     SKKRHFITAFRRYSMKNSGKNTHAALSELVSCFPQNAALLAGLGRAAALQALPLRLLA
                     SLNCGLENFEIRTPIGLLSDYGYFDIVVSS"
BASE COUNT         1142 a         1038 c         1130 g          897 t
ORIGIN      
        1 aaaaaaaccg tcaaattcca attcccgccc gaagtcgtgc gcgtccttgc aggcatgggt
       61 aacaacctga accaaatagc caaggccctg aacaccgccg caaaggtcgg cacgttgggc
      121 aatgtggaag cactcaaggc gacgaccgag ctggcagcgt tggaacgttc cttaaattcc
      181 ctacgggatt ttttagccaa agaaaagaac ggatggcagt cccaatgatt gtgcagtttt
      241 tcaatagggg gaaaggcggc gggagtggtc cgatagacta tcttctaggc aaagaccgcg
      301 accgagaaga agccagatta ttacgcggcg accccgaaga aaccgccgcc ctgataaaca
      361 gcagcgatta cgccaagaaa tacaccgccg gctgcctgag ctttgaagaa agcaacatcc
      421 ccgccgaaca gaaacacgcc ctgatggaca gcttcgaaga gtgtattttt gcaggcttgg
      481 acaaagacca atacaactgc ctatgggtag aacaccgaga caaagggcgt ttggaactca
      541 acttcgtgat accgaacatc gagcttttga gcggaaagcg gttacagccc tactactacg
      601 ccgccgacag aggaagagtg gacgcatggc gcaccatgca gaacctgacg cacggataca
      661 gcgacccaga cgaccccgcc aaacggcaga gcatgaccca agccaaagac ctgccgagaa
      721 acacgcagga agccgcacag agcatcacag agcatcacag acggcttaga agccctagcc
      781 ctatcaggca agctaaaaag ccgcgcagac gtgctggaaa cgctggaaaa ggtaggtttt
      841 gaaatatcac gagcgaccat cagcagcatc agcatcaaga acccggaccc aaaagggcgc
      901 aacatccgac tgaaaggcgc actgtatgag caagatttcc gatttggcga agaccttcga
      961 gcagacatca cgcaccgaag ccgccagcat agagcaacaa acgaaagcag acttagagac
     1021 gttacggaaa aatatcaacg aggcattgaa gcaaagcgag cagaaaataa ccgccgatat
     1081 aaacgcccgg cagttacgca tgagcaaggc agtattcaag ccctatctgt ggagcttgct
     1141 aggtatatcg gcggcagggt tgatagtcat agcagggctg ttcatagcga tatggagcgt
     1201 caagaacgag ctggacgact tgaaacagca gagagccgaa gcagagcgca ccctagacct
     1261 gttggaaacc aagaccaaag gtttgacact ggaaaattgc ccagtcgaga acagcaaagc
     1321 aacgcgggta tgcgtagcga ccgagaagcg aatgctggac gcgttagcgg aattagagag
     1381 caatcacgca gcaatcgagc agcgaatgat gaaagcctta acgcacttgg gcgaaaggtt
     1441 ggcagagcta gagcaggaaa acacgagttt agcgcagcag ctagcgagct tggcagccga
     1501 gttagagcgg cagagcgaaa tacggcaacg gcagagcgaa atcttgaatc aaccagccaa
     1561 acgataagcc aacgatacaa acgaacccaa agcaggggat ggggaatgag ccgatgatta
     1621 ccgagaacga acgcgacagg cgaacagccg catggctgat agagacctac ggggcagaag
     1681 ccgtagcgga agcagaaacc cgcattgcgg gcgcgagaaa gccctatccg agcaatatcg
     1741 ccaaagtatt gggggctagc ctacccgaag ccctaaaacg cacagaaaac gccgcagcgc
     1801 gccaaaaact ggcagggctg cggcggatgt tggacggtaa ggcagttaag acttcacaag
     1861 acttgtgaaa tcagaccact gctcaatgcg gaacgcccga atatcgcgga cagaagacgg
     1921 aaaccaaggc agagctttta gctcgttgat ggctgaaaac aggttagcca tatcttcgtt
     1981 ttggcaggtg tacaaacttc cctgaatgcg ggtaaagccg aatttccgca gggtgtatcc
     2041 aatatccgcg taggcttggg aaatgccttt cggatggttt tgagcggtat cggcaaccac
     2101 caaatcaaaa gaaatcgcgt acattaggca gcctttgggt cttgaacgat gtgggacagt
     2161 ttgtattgcg tgtgttcgcc gctttccggc agttcaatat ttacgagcca atcgccgtcc
     2221 ggaagctgtt cggcaggcgt gccgacgatg tacatcacgc cgtaatcgcc aaaggtgcgg
     2281 attgaaccgg tggggatagt ggtttgcatg gttagttctc ctgttgggtt tggttttcag
     2341 gctggtttgt catgtacaaa acccgttggg cagctttttc ggctgttgtg cgtttgatgc
     2401 cgtcaggcag aaaaattctc cattttttgg ctagaactat atctgcaaga tcatcaaccg
     2461 ttgcgctttt caaagcagga tttgcgttaa tttccgaata cagaaaatct ttgaatgccg
     2521 agtacagagc atcgcggttt attgcgtatc ttgtcatgct gtttcgcttt ggttgtcagt
     2581 ttcagacggc ataggcttgc cgcgcctgta ccaagtggcg cgggaaatgc cgaagtgttc
     2641 ccacggttta tcgcggctga tagagttttc ggctaggtag tcggcgcggg attgcgacca
     2701 tgcccgaaaa acggttcttg tggaacagcc gaagcgtttt gccagttctt tagccgtaac
     2761 gtctcgcttg gtggttttca gttttggata ggccatattt caaagctccg tgatgatttt
     2821 tgttctgcct gcattgatgg ttgttttccg caaactattc cgatgtgcct gcaattccga
     2881 aaaccgtcga ttgctggctg caatatcaaa cttgtaccaa acccagttag cgattgattt
     2941 ggagatacat ttcacttctt tttcccacat cgggacaggg aaatccccat ttacccgcat
     3001 acactggtaa tgcacttctt tcagccagcc ttgcaccgtg tagccctgct gtttgaacgc
     3061 caacacgttt ttgtgcgccc aacggctcac aaggttaaac accgtgcaat ttctgcttaa
     3121 tccgaccgct tccacgttag agcgaccgat atagggctta aacttgtcta aatccacgaa
     3181 atccgcaaga tactccaaat cgtagcccct gattgcgtca ggaacgccgc gcagcgtcag
     3241 ccaatgcgga tgttcgggat ttttcgtaat cagcgataca aagcccacat caccgcgcaa
     3301 tttcgcctta tatgctgctt caagtgcagc aagatagcgc agggcttttt gtctcccacc
     3361 gtattccgcc gtcagcacag gcgcggaaag cgcataggca aggtgtgcgc cgccgttttc
     3421 cctgttgatt gccgcccaag caggcatagg cagattattg tcttcccaag ccaaccccgc
     3481 cccttcgtaa tccaagtcaa agagcataaa cacacgcaga tgcggcggat tgacttggat
     3541 gtagcggcgt ttgatggcgg cagcgtaaga gcgcaccagc ataggcgctt ctttgaaatc
     3601 tttgcagtat ggcttgtgtg ggatacgttc ttgcaagaag aggtcgggtt gggtgtataa
     3661 ttggctcatg ttgtatctcg aaacccccgt gcagattggc gtttggcggg ggttttgctt
     3721 tgtctaagat ttgcagattg tatgcttgtt tttaagatga tacaactatg tcaaaataac
     3781 cataatcaga taacagcccg ataggggttc ttatttcaaa attttccaat ccgcaattta
     3841 gcgaagccag caggcgaagc ggtaaagctt ggagcgcagc agcgcgacct aagccggcca
     3901 gcagggcggc gttttggggg aaacatgaaa ccagttccga cagggcggcg tgcgtgttct
     3961 tccctgagtt cttcatggag tatcggcgaa atgccgtgat gaaatgccgt ttttttgagc
     4021 agaaagcagt caaaaacagg ggtattttgc ccttttgaca ggtttgagtg ccgccgaaaa
     4081 gcgaacaaag caactcatca tccgagtcag cccgaccgag tttgagactt tgacccgaca
     4141 gaagacccat ccgaatttag cccgctacat tcgggagcgg gttttggaag atggcaaagc
     4201 atccgac
//
LOCUS       DAXDXW010000111         4128 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXDXW010000111 DAXDXW010000000
VERSION     DAXDXW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.112"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            166..1086
                     /gene="ribF"
                     /locus_tag="ACFB64_002003"
     CDS             166..1086
                     /gene="ribF"
                     /locus_tag="ACFB64_002003"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC1210289.1"
                     /translation="MKIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVAVVFEPQPKEFFAARTGKTPPCRISPLRTKLGLLEKTGCVDAAWVLRFDRHF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLGGRVVHGRKLGRTLNAPTANIRLPGHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVGGGRFQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRRIEADMEAAKCW"
     gene            1228..4017
                     /gene="ileS"
                     /locus_tag="ACFB64_002004"
     CDS             1228..4017
                     /gene="ileS"
                     /locus_tag="ACFB64_002004"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC1210290.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKCEPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDNPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVAYPFKDTVA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     GALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWPEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGSDGKTLRDKAIKAVDDTEFFPPWG
                     RARLESMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGGYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PAINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKAGDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT          995 a         1180 c         1111 g          842 t
ORIGIN      
        1 acatagcacc gcgcctgctg cgcgttttat gcgtttggcg cgttcggcgg cgggaaattt
       61 gcctactttt cccgcgtcgg gcgggcggaa cgggcggcac actgtctata aaccgcaata
      121 ccgtttacaa tgaccgcctg tttcaccaca tacccgaacg caacaatgaa aatcaggctg
      181 gggcggcaca acgcgcccga ctttccgcac ggggccgccg taaccatagg caatttcgac
      241 ggcgtacacc tcggacacaa acacatcctc caaaaactcc gcctcgaagc cgatacgcgc
      301 ggattacccg tcgtggccgt cgttttcgaa ccccaaccca aagaattttt tgccgcccgc
      361 acaggcaaaa ccccgccgtg ccgtatcagc cccctgcgta ccaaactcgg gctgctcgaa
      421 aaaaccggtt gcgtcgatgc cgcctgggtt ttgcgtttcg accggcattt ttccgaaata
      481 tccgcgcagg ggtttatcga ccgcctgctg cgtcaaactt tgaatacgcg ctatttgctc
      541 gtcggcgatg attttcgttt cggcgcgggg cgcgaaggct gttttgaact tttggcacaa
      601 cagcccgata tgcaaaccga gcgcacgcct tccgtcatcg tcgaagacat ccgcaccagc
      661 agcacagccg tccgccaagc cctttcagac ggcaaccttg cctatgcgaa gaaacttttg
      721 gggcacgact acgttttggg gggcagggtg gtgcacggca gaaaactcgg acgcacctta
      781 aacgccccga ccgccaatat ccggctgccc ggccaccgtt atgccctcgg cggcgtgttc
      841 gtcgtcgaag cggacggcgc attcggcacg cggcgcggcg tggcgagctt cggcttcaat
      901 ccgaccgtcg gcggcgggcg ttttcaaaag ctggaagtcc atttgttcga ctttcaaggc
      961 gatttgtacg gacaacggtt gaacgtccgc ttcctgcaca aactgcgcga cgaggaaaag
     1021 tttgacggta tggaagaact gaaaaggcgg attgaagccg atatggaagc cgcaaagtgt
     1081 tggtagaaaa accttataca aaccatccga ttgggctaca atcagccttt taactgttca
     1141 gacggcatag gggtttcccg ttgtgaaata ctgtttgagg cgcaatgccg tctgaaaccg
     1201 aaatattgta acaatagaga ttaaaaaatg accgattaca gtaaaaccgt caacctgctc
     1261 gaaagcccgt ttccgatgcg cggcaatctt gccaagtgcg aacctgcgtg gctgaaaagc
     1321 tggtacgagc aaaaacgtta ccaaaaactg cgcgaaatcg ccaaaggccg tccgaaattc
     1381 atcctgcacg acggcccgcc gtatgccaac ggcgacatcc atatcggtca tgccgtcaat
     1441 aaaatcctta aagacattat tatccgcagc aaaacccaag ccggttttga cgcgccttat
     1501 gtaccgggtt gggactgcca cggcctgccc atcgaagtga tggtggaaaa actgcacggc
     1561 aaagatatgc ctaaagcccg tttccgcgaa ttgtgccgcg aatatgccgc cgaacagatt
     1621 gcccgtcaga aaaaagactt tatccgcttg ggcgtgttgg gcgactggga caatccttac
     1681 ttgaccatgg atttcaaaac cgaagccgat accgtgcgta tgctcggcga aatctacaaa
     1741 tccggctatc tctaccgtgg tgcgaaaccg gttcagtttt gcttggattg cggctcttcg
     1801 ctggcggaag cggaagtgga atacaaagac aaagtatcgc ctgcgattga cgttgcctat
     1861 ccgtttaaag acaccgtcgc gcttgccgcc gcattcggct tggcaggtat cgaaggcaaa
     1921 gcgtttgccg tcatttggac gaccacgcct tggactctgc ctgcgagcca ggccgtgtct
     1981 gccggcgcgg acgtggtgta tcaattaatc gatacgccca aaggcaaatt ggtgctggcg
     2041 aaagatttgg cggaaggcgc tttgaaacgc tacggctttt cagacggcat cgccatcctt
     2101 gccgaaacca ccggcgacaa gctggaaaac ctgcacatga atcatccgtt cctcgaacgc
     2161 gatattccca tgctcaacgg cgaacacgtt accaccgatg ccggtaccgg cttggtgcat
     2221 actgcgcctg cgcacggttt ggaagactac gccgtctgca ataaatacgg catcgagctt
     2281 tacaaccctg tcaacgccga aggcaaatac ataagcgaaa cgcctcgtgt cgcaggcatg
     2341 agcgtttggg aggcgaatcc cgtcatcctg caatggccgg aagaaaccgg caacctcttg
     2401 gcaagcagca aaatcgaaca cagctacgcc cactgctggc gccacaaaac cccgctgatt
     2461 taccgagcga caggtcagtg gtttgtcggc atggacaaag ccggcagcga cggtaaaacc
     2521 ctgcgcgaca aagccatcaa agccgtggac gacaccgaat tcttcccgcc atggggtcgt
     2581 gcgcgtttgg aatccatgat tgaaggccgt cctgactggg tggtttcacg ccaacgctat
     2641 tggggcacgc cgatgacttt ctttgttcac aaagaaacgg gcgagctgca tccgaactct
     2701 gccgaacttt tggaaaaagt cgcgcaacgc atcgaagaaa aaggcatcga ggcttggttc
     2761 tccctcgata aaagcgaatt attaagcgcg gaagattgcg aacattacga caaactcccc
     2821 gataccatgg acgtatggtt cgactcaggc tcgacgcatt attccgttgt aaaacaacgc
     2881 gaagaattgg aatggccggc tgacttgtac ctcgaaggca gcgaccaaca ccgcggctgg
     2941 ttccaatcct ctatgctgac cggttgcgcc tcatccatgg gacgcgcacc gtataaacag
     3001 ctgctgaccc acggtttcgt ggttgaccaa aacggccgca aaatgtcgaa atccatcggc
     3061 aacgtcgtcg cgccgcagga agtctataac gaattcggcg cggacatcct gcgcctgtgg
     3121 gcggcatcca ccgattacag cggcgaattg gcgatttcca aagaaatcct caaacgcgta
     3181 accgaaagct accgccgcat ccgcaatacc ttgagcttcc tgtttgccaa cctcagcgac
     3241 ttcaacccga ttgaagatgc cgtgcaacag gcggatatgg tggaaatcga ccgctacgcc
     3301 ttggtattgg cgcggcggct gcaagagcgt ttggcaggcg gttactatcc gcgctatgcc
     3361 ttccacttcg ccgtgaaaga cattgtttct ttctgctcgg aagacttggg cgcgttctac
     3421 ctcgacatcc tgaaagaccg cctctacacc accaaagccg acagccgcgc ccgccgcagc
     3481 gcgcaaaccg ccctgtacca catcacgcgc agcctggttc tcttgattgc accgattttg
     3541 tgcttcaccg gcgaagaagc gtgggacatc atcggcggcg gcgaagaaga cagcgtcctc
     3601 ttccatactt ggcacgagtt cccggccatc aacgaaaaag ccgaagccga actggtgaaa
     3661 aaatggacgg caatccgcga agcgcgcgaa gcagtaaccg ccgccatcga gcctttgcgc
     3721 gccgacaaaa ccgtcggttc gtccttgcag gcggaagccg aaatcaccgc gccggaagaa
     3781 atggccggct atctgaatgc tttgggcgaa gaattgcgct ttgccctgct ggtgtctaaa
     3841 gcagaagtga aagtcggtga tgaacttgcc gttgccgcca aagccggcga cggcgaaaaa
     3901 tgcgaacgct gctggcacta cacccgcgat gtgggcgcgg ttgcaggtta tgaaaccgtc
     3961 tgcaaacgct gtgcggagaa tgtcggcgga gaaggcgaaa cgcgccatta cgcctgataa
     4021 agtttgagca aatgccgtct gaaaccgcca aacagtattt cagacggcat tttttgtgcc
     4081 gcgatttgtc ttcataatgg cggaggggtt tcgagattgc ggtgttgt
//
LOCUS       DAXDXW010000112         3938 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXDXW010000112 DAXDXW010000000
VERSION     DAXDXW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.113"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(91..525)
                     /gene="queD"
                     /locus_tag="ACFB64_002005"
     CDS             complement(91..525)
                     /gene="queD"
                     /locus_tag="ACFB64_002005"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC1210291.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGTIKGG
                     AKDGMVMDFTDLKAIVKQHITDHFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYCRLKNAGLNVCRVKLWETPTSCAEYEGGEGIF"
     gene            complement(613..1128)
                     /locus_tag="ACFB64_002006"
     CDS             complement(613..1128)
                     /locus_tag="ACFB64_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC1210292.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DTDKIHIDGWQVVEWADGYDIAVSETPKTKMPPENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDYVRQHKDNLKDVDNLLALEHIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            complement(1156..1815)
                     /gene="queC"
                     /locus_tag="ACFB64_002007"
     CDS             complement(1156..1815)
                     /gene="queC"
                     /locus_tag="ACFB64_002007"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC1210293.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFRYGQRHA
                     VELERAEWIAQDLGVSQTVLDLSLMRQITHNALMDETAAIETADNGVPNTFVDGRNAL
                     FLLYAAIFAKGQGIRHIIAGVCETDFSGYSDCRGVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYKGIVGGCRECPSCILRERGLAECLESKKA
                     V"
     gene            complement(2000..2205)
                     /locus_tag="ACFB64_002008"
                     /pseudo
     CDS             complement(2000..2205)
                     /locus_tag="ACFB64_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            2304..3530
                     /locus_tag="ACFB64_002009"
     CDS             2304..3530
                     /locus_tag="ACFB64_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC1210294.1"
                     /translation="MTFLQRWQGLADNKICAFAWFVIRRFSEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYIDAFRDQANRLTA
                     IGSVMLVVTSLMLIRTIDNAFNRIWRVNTQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDSVLSSGAQQWADALKTAARLAFMTLLLWGLYRFVPNRFVPARQAFVGALITA
                     FCLETARFLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGRTLSVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELSELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(3551..3832)
                     /locus_tag="ACFB64_002010"
     CDS             complement(3551..3832)
                     /locus_tag="ACFB64_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC1210295.1"
                     /translation="MIREEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEAASHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
BASE COUNT          785 a          994 c         1129 g         1030 t
ORIGIN      
        1 aatcgttctc tttgatctga ggcgaggcaa cgccgtaccg gtttaaagtt aatccaccat
       61 accttatttg aattgactat atcgatacgt tcaaaatatt ccctcccccc cttcatactc
      121 cgcgcacgat gtcggcgttt cccacaattt gacgcggcac acgttcagcc ccgcgttttt
      181 cagacggcag tacatttcga ccgccatatt ttcggcagtt gtgcggcagg gcaggcgcag
      241 ggttttcatg ttccagcctt ccaaaagcgc ggcgatttga gattcgcgcc cgttgccgcc
      301 gtggtagatg aaggcgtggt cgaaatggtc ggtaatgtgt tgtttcacaa tggctttcaa
      361 gtcggtaaag tccatcacca taccgtcttt cgctccgcct ttgatggtgc cgtctgaaac
      421 ggtgatttcg agtttgtagg tatgtccgtg caggttttgg catttgccgt catgcccgtc
      481 gagcatatgc gaggagtcga aggtaaatat cttggtaatt ttcatcccga ttccttttca
      541 gtgaaaaccc cgccgctttc cgcccggagg gtggcttgat gtgtcgtttt gcgtggaaac
      601 atatccgctg cttcaaatgg gcaaatagcc ttgaaagccg atttcggcgg gtttgccgtg
      661 cggattcggg gtcaggcgga tatggaaatt gccgatgtgc tcgagcgcaa gcaggttgtc
      721 cacgtctttc aagttgtctt tatgctgccg aacatagtcg gtcaacaggg tttgcagggc
      781 tttttgtttg gcttcggcag gcgtggcggc ggcgaacagc ccgaacgcgt gcgcctcggc
      841 aagctgcccc gcgcgatagc cgccgacatt ggcaaaatac aggcgcggag cgttttcagg
      901 cggcattttc gttttgggcg tttcggatac ggcgatgtcg taaccgtccg cccattcgac
      961 aacctgccaa ccgtcgatgt ggattttgtc cgtatcgccg aaccacgcgg ctttgagtgc
     1021 ggggacggct tcgcggtagt cgtcgcacac ggcaaattgg atgtcgtgca cttcgatatt
     1081 cgaccggccg gcattgccgc cgaggtaaaa catatggagc ttgggcatat cgtattcctt
     1141 gtgctttgcg cgcgttcaga cggccttttt actttccaga cattccgcca gcccgcgttc
     1201 gcgcaagata cagctcgggc attcgcggca gccgccgacg atacccttgt agcaggtgtg
     1261 ggtctgttcg cggatgtagt ccaacacgcc catttcgtcc gccaacgccc acgtttgcgc
     1321 cttggtcagg tacatcagcg gcgtgtggat ttgaaaatcg tagtccatcg ccagattaag
     1381 ggtaacgttc atggatttga cgaacacgcc gcggcagtcg gaatagcccg aaaaatcggt
     1441 ttcgcacacg cccgcgatga tgtgccggat accctgccct ttggcaaaaa tggcggcgta
     1501 aagcaggaaa agcgcgttac gcccgtccac aaaggtattg ggaacgccgt tgtcggcggt
     1561 ttcgatggcg gcggtttcgt ccatcagggc gttgtgcgta atctgccgca tcaggctcaa
     1621 atcgagtacg gtttgactga cacccaaatc ctgcgcgatc cactctgcgc gttccagctc
     1681 gacggcatgg cgttgcccgt atcggaaggt gatggcttgg acgttttcgc gcccgtaggt
     1741 ttggattgcc tgaatcaggc aggtggtcga atcctgaccg cccgagaaga tgaccaaggc
     1801 tttttggttt gacatatcga atcctagttt tggtaacgcg ggaggtttgc gaaccgcgaa
     1861 gttggacagt aaagacgcgg attatacagg caaacgcgtt cagacggcat ctgaacgcca
     1921 tgccgtctga acacgcggcg tatgccgact tgacggcgag ttccgccaag cgtctgcctt
     1981 gtgcgaaggc gatattgttt tcttcggcgg tcaggacggg agccgctgtc gggaatatct
     2041 ttatttgacg gcttcgcaga cggcggacac tttggggacg gtgcgcaata cggcttcgca
     2101 accttcaacg ctgtcgatgc cgcgagtgat tcggcgtgcg ggattgcggg tgctgccgtt
     2161 ttgggaatag tagaggacga ggattttgag gggatttggg ttcatgggcg gattccgtta
     2221 aaatagtttg gtgttcagac ggcataacgg ttgtccagat gccgtctgaa acagtttgaa
     2281 aacatgaaac ctggtaagag attatgacct ttttacaacg ttggcaaggt ttggcggaca
     2341 ataaaatctg tgcatttgca tggttcgtca tccgccgttt cagtgaagag cgcgtaccgc
     2401 aggcagcggc gagcatgacg tttacgacac tgctggcact cgtccccgta ctgaccgtaa
     2461 tggtcgcggt cgcttcgatt ttccccgtgt tcgaccgctg gtcggattcg ttcgtctcct
     2521 tcgtcaacca aaccattgtg ccgcagggcg cggatatggt gttcgactat atcgacgcat
     2581 tccgcgatca ggcaaaccgg ctgaccgcca tcggcagcgt gatgctggtc gtaacctcgc
     2641 tgatgctgat tcggacgata gacaatgcgt tcaaccgcat ctggcgggtt aacacgcaac
     2701 gcccctggat gatgcagttc ctcgtttatt gggcgttgct gactttcggg cctttgtctt
     2761 tgggtgtggg catttccttt atggtcgggt cggttcaaga ctccgtactc tcctccggag
     2821 cgcaacaatg ggcggacgcg ttgaagacgg cggcaaggct ggctttcatg acgcttttgc
     2881 tgtgggggct gtaccgcttc gtgcccaacc gcttcgtgcc cgcccggcag gcgtttgtcg
     2941 gagctttgat tacggcattc tgcctggaga cggcacgttt cctgttcacc tggtatatgg
     3001 gcaatttcga cggctaccgc tcgatttacg gcgcgtttgc cgccgtgccg tttttcctgc
     3061 tgtggttaaa cctgctgtgg acgctggtct tgggcggggc ggtgctgact tcgtcgctgt
     3121 cttattggca gggcgaggcc ttccgcaggg gattcgactc gcgcggacgg tttgacgacg
     3181 tgttgaaaat cctgctgctt ctggatgcgg cgcaaaaaga aggccgaacc ctgtccgttc
     3241 aggagttcag acggcatatc aatatgggtt acgatgaatt gggcgagctt ttggaaaagc
     3301 tggcgcggta cggctatatc tattccggca gacagggctg ggttttgaaa acgggggcgg
     3361 attcgattga gttgagcgaa ctcttcaagc tcttcgtgta ccgcccgttg cctgtggaaa
     3421 gggatcatgt gaaccaagct gtcgatgcgg taatgacgcc gtgtttgcag actttgaaca
     3481 tgacgctggc ggagtttgac gctcaggcga aaaaacagca gcagtcttga gtatttttga
     3541 aactgtattt ttatcctaaa gacattttcc tatctgctga ttccaccgtt tcctttctgt
     3601 gtgccgccat aatttttata atagacaagc cattcaagtt tgcattgact gctttttcat
     3661 tggcaatatc caaatgactg gccgcttcat ctaaaaatag gattttcggt tcgcaatata
     3721 aggcccgcgc caatacgata cgttgttttt gtccgcctga cagtgcgctg cccatatcgc
     3781 cgatcaaggt ttcatagccc ataggcatgg cggagatttc ttcgcgaatc atagcaaggc
     3841 aggcgcaatg ttcgattttt cctctatccg gttctgtatc gaaaaaacag atattctctg
     3901 caattgagcc agcaaatgca aatatagtgg attaaatt
//
LOCUS       DAXDXW010000113         3606 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXDXW010000113 DAXDXW010000000
VERSION     DAXDXW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.114"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..430
                     /locus_tag="ACFB64_002011"
     CDS             <1..430
                     /locus_tag="ACFB64_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC1210296.1"
                     /translation="IYKVWQFVEDALRAVVPADSFEPTAQKLKLFKVGAATILFYEDQ
                     NVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGAGANLQHYNPLPDVAIAK
                     AWNIPENWLLRAQMVIGGIEGAAGEKVFEPVAERLKVFGA"
     gene            complement(485..940)
                     /locus_tag="ACFB64_002012"
     CDS             complement(485..940)
                     /locus_tag="ACFB64_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HFC1210297.1"
                     /translation="MNRRQFLGSAAAVSLASAASFARAHGHADYHHHHDMQPAAASAY
                     TAVRQTAAHCLDAGQVCLTHCLSLLTQGDTSMSDCAVAVRQMLALCGAVHDLAAQNSP
                     LTRDAAKVCLEACKQCAKACKEHAAHHAECKACYESCLDCIKECEKLAA"
     gene            complement(1196..2566)
                     /gene="ffh"
                     /locus_tag="ACFB64_002013"
     CDS             complement(1196..2566)
                     /gene="ffh"
                     /locus_tag="ACFB64_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC1210298.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNQALVELMGKENKTLDLSVSPPAI
                     ILMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAVVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLNGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKAGRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRLAKGMKGMK
                     GMFPGL"
     gene            2788..3594
                     /locus_tag="ACFB64_002014"
     CDS             2788..3594
                     /locus_tag="ACFB64_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC1210299.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDFPFMLHIGTSLLAYGLFGIATLLSVLSLLLHRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
BASE COUNT          788 a          892 c          968 g          958 t
ORIGIN      
        1 aatatataag gtgtggcaat ttgtcgaaga cgcgctgcgt gccgtcgtgc ctgccgacag
       61 ttttgaaccg accgcgcaaa aattgaagct gtttaaggtg ggcgcggcaa ccattttgtt
      121 ttatgaagat caaaatgtcg tcaaaggttt gcaggagcag ttccctgctt atgccgccaa
      181 ctttcccgtt tgggcggacc aggcgaacgc tatggtacag tatgccgtct ggacgacact
      241 tgccgcggtc ggtgcaggtg caaatctgca acattacaac cccttgcccg atgtggcgat
      301 tgctaaagcg tggaatattc ccgaaaactg gctgttgcgc gcgcaaatgg ttatcggtgg
      361 tattgaaggg gcggcaggtg aaaaggtctt tgaacccgtt gcggaacgtt tgaaagtgtt
      421 cggcgcataa tttcgcggtc aaaaaaatgc cgtctgaacc ccgttcagac ggcatttttc
      481 agtatcaggc ggcgagtttt tcgcattctt tgatacagtc gaggcaggac tcgtaacagg
      541 ctttgcattc cgcatggtgg gcggcgtgtt ctttacaggc tttggcacac tgtttgcacg
      601 cttcgaggca cacttttgcc gcgtcgcgtg tcagagggga attttgtgcg gcaaggtcgt
      661 gcaccgcgcc gcataaggca agcatctggc gcacggcaac cgcacagtcg gacatagacg
      721 tgtcgccctg agtgagcagg gacaggcagt gggtcaggca aacctgtccg gcatccagac
      781 agtgtgcggc agtttggcgg acggcggtgt aggcggatgc ggcggcaggc tgcatatcgt
      841 gatgatggtg gtagtcggcg tgtccgtgcg cacgcgcgaa agaggcggcg gaagccaaag
      901 agacggcagc ggcgctgccc aaaaattgac gacggttcat gattttttcc tttatgtttc
      961 aacatgcggg tccggtataa ggtacacact tactatactg ccctggccgg atcggtacga
     1021 ttttctgaat aattgcttga ttttgcaggt gtgaaattgt cgcacgagca ggcatgtttt
     1081 agctattcgg aaattcgagg aagggtgcat caacctgttt tgaaatgatg cgcagacatg
     1141 gtaacagaca aaaagccgtc tgaaaatcgt tttagacggc ttttgttttc aaacattaaa
     1201 gaccgggaaa cattcccttc attcccttca tgcctttggc caagcgcatc agtttgccca
     1261 aaccgttgcc gctgaacatc ttcatcatct gctgcatttg ttcaaactgt ttgagcaatt
     1321 tgttcacttc ctgcacggtc gtgcccgcgc ccatcgcaat acggcgtttg cggccggctt
     1381 tgagcagggc agggttggcg cgttctttag gggtcatcga gttgatgatg gcttctactt
     1441 tgcccatcgc tttttcagcc gtaccttcgg ggatttgttt cgatatttga cccagttcgc
     1501 ccggcatttt cgacatcagg ttttccaaac cgcccatatt gcgcatttgc tggatttgtt
     1561 ctttaaaatc attgaggtca aagcctttgc ctttttgcag ctttttcgcc attttggcgg
     1621 cggcttcttc atcaatacct ttttgaacgt cttcaatcag ggtcaatacg tcgcccatgc
     1681 cgaggatacg gctggcaaga cggtcggggt ggaaaggttc gaggccgttg attttctcgc
     1741 cgacgccgat aaatttaatc ggtttgccgg ttacatggcg tacggacaat gccgcaccgc
     1801 cgcgcgagtc gccgtccatc ttggtcaata cgacgccgtt cagcggcagg gcttcattaa
     1861 atgcctgagc ggtatttacc gcatcctgac ccagcatggc atcgataacg aacaaagttt
     1921 caaccgggtt gaccaccgca tgaagcgctt tgatttcgtt catcatctct tcatcgattg
     1981 ccaaacggcc ggcggtatcg accatcaata catcgtaaaa atgttttttg gcgtaatcaa
     2041 cggcggcagt tgcaatctca accggttttt ggttggtatc ggatgggaaa aaatctacgc
     2101 cgacctgttc ggccaacagg cgcagctgtt caatcgcggc agggcggtaa acgtcggcgg
     2161 ataccaccaa aacttttttc ttctgatcgt ttttcagcag gcgggcgagt ttgccgacgg
     2221 ttgttgtctt accggcaccc tgcaaaccgg ccatcaaaat gatggcaggc ggtgaaaccg
     2281 ataaatccag tgttttgttt tccttgccca tcagttcgac taaggcttgg ttgaccacgc
     2341 cgataaaggc ttggtcaggc gtcaggctgc ccgccacttc ctgaccgagg gccttttctt
     2401 tgacgttgtt gacgaactct ttgacgacag gcagggcgac atccgcctca agcagggcga
     2461 gtcggacttc gcgcaaggct tctttaatat tgtcttcggt cagtttggcc tgcccccgga
     2521 tgtttttgaa gacattgctg aagcggccgg ttaaattgtc taacatactt ggtccttggt
     2581 ctgaagtaag aatagcttgc cccatcaggg gcattctttg ttaaaataaa atcaaaataa
     2641 tttgatgcgg cttgtgtgcc ggacagcata tcggcaaatc cgtcaaggct tgcccgaaac
     2701 ggggatttta caattccaac gttaaaagtt ccaatatttc ataagcggcc gcatacggcg
     2761 caacagtata gatagagaaa gtccaccatg ccgacagttt tcatcttttt gacggcggtt
     2821 tacgcaggat tgggtgcatt tgcatggcac tgccaacagc aggggcgcgg ccgggattac
     2881 ccgtggaaga cggaattgcc ggttttgggc gcggcattga ccgtccacgg cgcggcactg
     2941 ctgatgccgg tcattcaaga caaaatcatc attatgggct tcgggtattc cggcagcctg
     3001 attgtttgga tgatgctgtt tatttatttt gccggcagct ttttttatcc gctgcgcgga
     3061 gtgcagttgc tgctgtatcc ttgcgccgca ctgatgctgc tgtcaggttt ggttttcccg
     3121 ggaaaattct cgggatatga aattaccgac tttcccttta tgctgcatat cggcacatca
     3181 ctgcttgcat acgggctgtt cggcatcgca acattattgt ccgttttgag cttgctgctg
     3241 caccggagcc tgcaccgcag aaacttctcc aagctcgcag gattcctgcc gtcgctgctc
     3301 agtttggaaa aactcatgtt ccaggccatg tgggcaggtt tcatcctgct gacctattcc
     3361 gtcgtcagcg gcacattctt tgccgaagcc gtattcggca aacccatgac atttacccat
     3421 aaaaccgtat tcggcatatt gtcatggctg atttacggag gactgctgct caaacacagc
     3481 atgaccgcat ggcgcggcaa aaaagccgcc gtgtggacca tcatcggatt tgtcagcctt
     3541 atgattgcct atatgggcag caagttcgta ttggaaatca ttctgaaaag ataagaagag
     3601 ccaaca
//
LOCUS       DAXDXW010000114         3561 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXDXW010000114 DAXDXW010000000
VERSION     DAXDXW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.115"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(308..523)
                     /locus_tag="ACFB64_002015"
     CDS             complement(308..523)
                     /locus_tag="ACFB64_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210300.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESVFL"
     gene            complement(554..3322)
                     /gene="tdfH"
                     /locus_tag="ACFB64_002016"
     CDS             complement(554..3322)
                     /gene="tdfH"
                     /locus_tag="ACFB64_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC1210301.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSGENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRGVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGGLKFNSNSGKWER
                     DFQRPYWKTKWYQKYNDPQELQKYIEGHDKSWRENLAPQYDITPIDPSGLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNAINYRFGGEYTGYYGSENEFKRAFGENSPAYKEHCDPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFAGYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDILGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVGST
                     GLAYTIRHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
BASE COUNT          826 a          872 c          888 g          975 t
ORIGIN      
        1 agtaaataaa aaataacaac ataaatcaat ttattacggt tttattttta tttggtgttg
       61 acgggcaaca tatcatctgc gcggtaatga tgagatttta ggttgtggga tttatcggaa
      121 aaacagaaac ctctccgccg ccattctcac gaaagtggga atccggaaag ctaaagccac
      181 aggaatttat cggaaatggc tgaaatttat agtggattaa caaaaatcag gacaaggcga
      241 cgaagctgca gacagtacaa atagtacggc aaggcgaggc aacgccgtac ctgtttttgt
      301 taatccacta taaaaaaact gattcccgct tttgcgggaa tgacgactca taaattaccc
      361 gaaacaacca aaaataaccg aaatcaaaaa ccggcttccc tgcgtgggaa tgaaatggat
      421 ttttctttga gttgccgatg cgataaaacg gattggcata ggcttgcggc aggatgatgc
      481 gtttggggaa acaaatgccg tctgaagggc tttcagacgg cattgcagtt tttccacaaa
      541 acgcgggctg cctttaaaac ttgtagctca tcgtcatcaa gaaggtgcgt ccgcgtgcga
      601 aattggtcaa tacgcttttg cttgtgccgc cgtatttgcc gttgcacaac gttttatcag
      661 cattacacgt tacgtcttcg tccttgtctt tcgggtcgaa cgagctgtaa taacgctgcg
      721 ttgccgcatc attgcccgca tcgagcggat cgatataacg cctgtcgaac aggtttttga
      781 cttcggcgcg gaaaataagg tttttcttcg gctcgtaagc ggcgtaaaaa tcaaaaatca
      841 aaggctgtcg ggcaagggtt tcggtttgtt tgatggaacg cttgcccagt tgccggacat
      901 tgctggtatt tcccccgttg gtgccgtcga tatagcgttc ttcagccgtc gcgcggatgc
      961 tcttgccgaa atagcgcatc gcgccgccca aagtcagttt gttgcccaac cagcgcgtac
     1021 cgacttccaa acgtccgtaa tctcgcggca gggcggaaac cctgctcagc ccataacctt
     1081 gtttgagttg gtcttctttg gaggcattgt tgggcgattc gctcgcatcg ctgaaattgg
     1141 tcggttgcgt gcttttttga taggcgtaag aaaggttggt gaaaaaacgc ccataatcgt
     1201 aattcagctc cagctcaaaa ccgtgtttgt gcactttgtc tttgaaattg cggtgtcgga
     1261 tggtgtaggc aagcccggtg ctgccgaccc agctcggaat atccccgttc aaatcccacc
     1321 atttcccgta aacgttgtgg atgtagttgt caatgcggct gcggtagccg accagtttca
     1381 atcctaatat atcatcttgt tttaacaatc cttttttata ggtattgaag ccaaattgcc
     1441 aagtgtttgc gcgctctggt tttaaggcgg tgtgaacgcc ggagtcgccg atttgggaaa
     1501 aatacatttc ttggatgttg ggcatacggt gtgtgcgcga atagccggcg aacggcatga
     1561 aataatcgcc gaagtccgca ctaatgctga ccgaatggtt gttggcgcgc tttttgccgt
     1621 attttttcaa tacgggttca taaagcccgc agctcgggtc gcaatgttcc ttgtatgccg
     1681 gcgagttttc tccgaatgcc cgcttaaatt cgttttccga gccgtaatag cccgtatatt
     1741 cgccgccgaa acggtagttg attgcattgg tgctgtagtt taagcggtaa atgtcttttt
     1801 tgagcgcggc atcgaagtag aacgtgttga aatattgact gccggccggt tggacgatgg
     1861 ttgatttttg gggcagcagc cctttatcgc ccttaaaccg ccccaaatag gaataaagcc
     1921 cgttgtcctg atccggaccg tcgaaaaaca gccccaattc ttcaggaaag cggtttttgc
     1981 cgtattcgtt gtggaaataa ttgaagccca aagtggtttg caactcggtt tcgcggggca
     2041 gccggaaggt ggcggtgttg ttgaggtcga ggatttttgc gttgttgtag gtttcaaaat
     2101 cctttaaaag cccccagcct gtaaacttcg accctttcgg atatttctgc ctgcccgaat
     2161 tgtaggctgc ggtcagattg aggttggtat acgggttcaa agacaaaccg taattgaatt
     2221 gataattgcg gttgatgatt ttgcggctgc cgattttggt gtttaaatcg cgaaattgcg
     2281 ccgtgtattt attgaatacg ccgtcgtatt ccaatttaaa cagattacct gccgactgct
     2341 gcttcaggcc ggacggatcg atgggggtga tgtcgtattg cggcgccagg ttttcccgcc
     2401 agcttttgtc atgaccttcg atgtattttt gcagttcttg ggggtcattg tatttttgat
     2461 accacttggt tttccagtac ggcctttgga aatcccgctc ccattttccg ctgttggaat
     2521 tgaatttcaa cccgccttct tgtacaaaat atcgctgctt gcgccgttcc agatattccg
     2581 cgccaaaatt tccgatgtgc tgcccgccgc cgcccacgcg gtaattttgc gccacgccgc
     2641 gcctgctgtg cccgtaaagc acaccgacag atgctccgct ttccagccat ttgcgcgcac
     2701 ctatcgccgc catcgcatta cctttggttg aattggtgcc ggtcagacct tttagcagca
     2761 ggccgtaggt attattgccc tgaacgacgt catccacgcc taaagtccgc agattcgccg
     2821 aaccggcaag gctgttgatg cctgccgagc cgctgaagct gcctttgacg acatccagtc
     2881 cggcaataaa attgctgtcg acagatgcac cgaattgaga tgaaccgcct gccctgcccg
     2941 catcggtaga agtcgaataa aaggtctgcg tgatgccgtc caccatcgta ttgacccgcc
     3001 cgaacccgct gtcgccgcga atattcaaag acacaatgcc cgagctttta tcttgctgtg
     3061 taaacgcacc gggtatgctg cgtacgatgt tgtcgaggtt ttcgccggat ttgaatatat
     3121 cctgacgggt cgatacggca cgcgcatcgg taaacacttt tttgtctttc ggtacgcgct
     3181 tcgccttgac gtgcacgtct tccaaaacct gtatctgcgc ctcgctgccc gcgcgccctg
     3241 catcttcggc ataactatga tgatatagca taacacccat aagataaaaa caaatcggct
     3301 tcaaccggaa agaagatctc atattttcct caacaataaa caatcaatcc aacatgtcta
     3361 ccgttttcat cgaatccgcc ctttcgacca cccggcccta cgcaaccgaa ccgtcattcc
     3421 cacggaagtg ggaatctaga atcttggggt ttcagtcatt tccgatagat tcccgccgcg
     3481 tcaggggtcc ggattcccgc ctgcgcggga atgacgaatt tcgagattgc ggtattgtcg
     3541 ggaatgacgg gtttcaagat t
//
LOCUS       DAXDXW010000115         3551 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXDXW010000115 DAXDXW010000000
VERSION     DAXDXW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.116"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            98..3297
                     /gene="nalP"
                     /locus_tag="ACFB64_002017"
                     /pseudo
     CDS             98..3297
                     /gene="nalP"
                     /locus_tag="ACFB64_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
     gene            3349..>3551
                     /locus_tag="ACFB64_002018"
     CDS             3349..>3551
                     /locus_tag="ACFB64_002018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210302.1"
                     /translation="MKRKPYSNCLLLLFQDFFQRFGINQTYSGLTKIRTRRRAAGSTN
                     GTEPIRPVLHHLGEPFPLSRGGAT"
BASE COUNT          952 a          971 c          967 g          661 t
ORIGIN      
        1 gatataattc aaaatataaa aatgggttac atctggacat tacgaaattt ttattccttc
       61 gcctgaattc tattgtcaga ttcaaagaga cctcatcatg cgaaccacct caaccttccc
      121 tacaaaaact ttcaaaccgg ctgccatggc gttagctgtt gcaacaacac tttctgcctg
      181 cttaggcggc ggcggaggcg gcacttctgc tcccgacttt aatgcaggcg gcaccggtat
      241 cggcagcaac agcagggcaa cgatagcgga atcagcagca gtatcttacg ccggtataaa
      301 aaacgaaatg tgcaaagaca gaagcatgct ctgtgccggt cgggatgacg ttgcggttac
      361 agacagggat gccaaaatca aagcccccga atctgcatac cagagacttt tcaaacccaa
      421 atgaccaata ttaagaatat gatcaacctc aaacctgcaa ttgaagcagg ctatacagga
      481 cgcggggtag aggtaggtat cgtcgataca ggcgaatccg tcggcagcat atcctttccc
      541 gaactgtatg gcagaaaaga acacggctat aacgaaaatt acaaaaacaa attacaaaaa
      601 ctatacggcg tatatgcgga aggaagcgcc tgaagacaga ggcggtaaag acattgaagc
      661 ttctttggac aatgaggccg ttatagagac tgaagcaaag ccgacggata tccgccatgt
      721 aaaagaaatc ggacacatcg atgtggtctc ccatattatt ggcgggcgtt ccgtggacgg
      781 cagacctgca ggcggtattg cgcccgatgc gacgctacac ataatgaata cgcatgatgg
      841 aaccaagaac gagaaataat gtctgcagcc atccgcaatg catgggtcaa gctgggcgaa
      901 cgtggcgtgc gcatcgtcaa taacagtttt ggaacaacat cgagggcagg cactgccgac
      961 catttccaaa tagccaattc ggaggagcaa taccgccaaa cgttgctcga ctattccggc
     1021 ggtgataaaa cagatgaggg tatccgcctg atgcaacaga gcgattaggg caacctgtcc
     1081 taccacatcc gtaataaaaa catccttttc atcttttcga caggcaatga cacacaagct
     1141 cagcccaaca cactgaccct attgccattt tatgaaaaga cgctcaaaaa ggcattatca
     1201 cagtcgcagg cgtagaccgc agtggagaaa agttcaatgg ctccaaccat tgcggaatta
     1261 ctgccatgtg gcgcctgtcg gcatcctatg aagcaaccgt ccatttcacc cgtacaagac
     1321 cgattcaagt tgccggaaca tccttttccg cacccatcgt aaccggcacg gcggctctgc
     1381 tgcagagata cccgtggatg agcaacgaca acctgcgtac cacgctgctg acgacggctc
     1441 aggacatcgg tgcagtcgga gtggacagca agttcggctg ggggctgctg gatgcgggta
     1501 aggccatgaa cggacccgcg tcctttccgt tcggcgactt taccgccgat acgaaaggta
     1561 catccgatat tgcctactcc ttccgtaacg acatttcagg cacgggcggt ctgatcaaaa
     1621 aaggcggcag ccaactgcaa ctgcacggca acaacaccta tacgggcaaa accgttatcg
     1681 aaggcggttc gctggtgtta tcggatatgc acgtcaaaac caaaggtgca ctgatttata
     1741 acggggcggc atccggcggc agcctgaaca gccacggcat tgtctatctg gcagataccg
     1801 accaatccgg cgcaaacgaa accgtacaca tcaaaaggcg gtctgcagct gggcgacaaa
     1861 ggtacgctgt acacacgttt gggcaaactg ctgaaagtgg acggtacggc gattaccgac
     1921 ggcaagctgt acatgtcggc acgcggcaag ggggcaggct atctcaacag taccggacga
     1981 catgttccct tcctgagtgt cgccaaaatc gggcaggatt attctttctt caaaaatatc
     2041 gaaaccgacg gcggtctgct ggcttccctc gacagcgtcg aaaaaacagc gggcagtgaa
     2101 ggcgacacgc cgtcctatta tgtccgtcgc ggcaatgcgg cacggactgc ttcggcagcg
     2161 gcacattccg cgcccgccgg tctgaaacac accgtagaac agggcggcag caatctggaa
     2221 aacctgatgg tcgagctgga tgcctccgaa tcatccgcaa cacccgagac ggttgaaact
     2281 gcggtcgccg accgcacaga tatgccgggc atccgcctac ggcgcacaac tttccgcgca
     2341 gcggcagccg tacagcatgc gaataccgcc gacggcgtac gcatcttcaa cagtctcgcc
     2401 gctaccgtct atgccgacag tgccgccgcc catgccgata tgcagggacg ccgcctgaaa
     2461 gccgtatcgg acgggttgga ccacaacggt acgggtctgc gcgtcatcgc gcaaacccaa
     2521 caggacggtg gaacgtggga acagggcggt gtcgaaggca aaatgcgcgg cagtacccaa
     2581 actatcggca ttgccgcgaa aaccggcgaa aatacgacag cagccgccac actgggcata
     2641 ggacgcagca catggagcga aaacagtgca aatgcaaaaa ccgacagcat tagtctgttt
     2701 gcaggcatac ggcacgatgt gggcgatatc ggctatctca aaggcctgtt ctcctacgga
     2761 cgctacaaaa acagcatcag ccgcagcacc ggtgcggatg aatatgcgga aggcagcgtc
     2821 aacggcacgc tgatgcagct gggcgcactg ggtggtgtca acgttccgtt tgccgcaacg
     2881 ggagatttga cggttgaagg cggtctgcgc cacgacctgc tcaaacagga tgcattcgcc
     2941 gaaaaaggca gtgctttggg ctggagcggc aacagcctca ctgaaggcac actggtcgga
     3001 ctcgcgggtc tgaagctgtc gcaacccttg agcgataaag ccgtcctgtc tgcgacggcg
     3061 ggcgtggaac gcgacctgaa cggacgcgac tacgcggtaa cgggcggctt taccggcgcg
     3121 gctgcagcaa ccggcaagac gggtgcacgc aatatgccgc acacccgccg ggttgccggt
     3181 ctgggggtgg atgtcgaatt cggcaacggc tggaacggct tggcacgtta cagctacacc
     3241 ggttccaaac agtacggcaa ccacagcgga caaatcggcg taggctaccg gttctgacgg
     3301 acagaaaaca gacagccgca aagatcacgg tctttgcggc tgtttcttat gaaaagaaaa
     3361 ccctattcca attgcctgct tctattgttt caagacttct tccaaagatt cggcatcaat
     3421 cagacgtata gcggattaac aaaaatcagg acaaggcggc gggccgcagg cagtacgaat
     3481 ggtacggaac cgatccgccc ggtgcttcat caccttgggg aaccgttccc tttgagccgg
     3541 ggcggggcaa c
//
LOCUS       DAXDXW010000116         3507 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXDXW010000116 DAXDXW010000000
VERSION     DAXDXW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.117"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            13..1809
                     /locus_tag="ACFB64_002019"
     CDS             13..1809
                     /locus_tag="ACFB64_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC1210303.1"
                     /translation="MQYKPLLLALMLVFSAPAVAAHDAAHNRSAEVKKQAKNKKEQPE
                     AAEGKKEKGKNAAVKDKKTGGKEAAKEFKKTAKNRKEAEKEATSRQSARKGREGDKES
                     KAEHKKAHGKPVSGSKEKNAKTQPENKQGKKGAKGQGNPRKGGKAEKDTVSANKKARS
                     DKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVSNSL
                     KQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEIAATKA
                     QISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKAL
                     AVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKISKDARKLLEQKG
                     NEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMS
                     NLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVFYS
                     TAPATVESIAPGTVSYADELDGYGKVVVIDHGENYISIYAGLSEISAGKGYTVAAGSK
                     IGTSGSLPDGEEGLYLQIRYQGQVLNPSGWIR"
     gene            1936..3411
                     /locus_tag="ACFB64_002020"
     CDS             1936..3411
                     /locus_tag="ACFB64_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC1210304.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDGRDNEVLPVQ
                     SIRTMAEVYGQIKANYYHDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAEVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDGMVLKAVPEDYVYGMGGDPLAGIPAELKTIPMAVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERT
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAAKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKK"
BASE COUNT         1031 a          812 c         1025 g          639 t
ORIGIN      
        1 gaaagccgaa ccatgcaata caaacccctc ctgcttgccc tgatgcttgt cttttccgcc
       61 cccgccgttg ccgcccacga cgcggcacac aaccgttccg ccgaagtgaa aaaacaggcg
      121 aagaacaaaa aagaacagcc cgaagcggcg gaaggcaaaa aagaaaaagg caaaaatgcc
      181 gcagtgaaag ataaaaaaac aggcggcaaa gaggcggcaa aagagttcaa aaaaaccgcc
      241 aaaaaccgca aagaagcaga gaaggaggcg acatccaggc agtctgcgcg caaaggacgc
      301 gaaggggata aggaatcgaa ggcggaacac aaaaaggcac atggcaagcc cgtgtccgga
      361 tccaaagaaa aaaacgcaaa aacacagcct gaaaacaaac aaggcaaaaa aggggcaaaa
      421 ggacagggca atccgcgcaa gggcggcaag gcggaaaaag acactgtttc tgcaaataaa
      481 aaagcccgtt ccgacaagaa cggcaaagca gtgaaacagg acaaaaaata cagggaagag
      541 aaaaatgcca aaaccgattc cgacgaattg aaagccgccg ttgccgctgc cactaatgat
      601 gtcgaaaaca aaaaagccct gctcaaacaa agcgaaggaa tgctgcttca tgtcagcaat
      661 tccctcaaac agcttcagga agagcgtatc cgccaagaac gtatccgcca agagcgtatc
      721 cgtcaggcgc gcggcaacct tgcttccgtc aaccgcaaac agcgcgaggc ttgggacaag
      781 ttccaaaaac tcaataccga gctgaaccgt ttgaaaacgg aaatcgccgc tacgaaagcg
      841 cagatttccc gtttcgtatc ggggaactat aaaaacagcc agccgaatgc ggttgccctg
      901 ttcctgaaaa acgccgaacc gggtcagaaa aaccgctttt tgcgttatac gcgttacgta
      961 aacgcctcca atcgggaagt tgtcaaggat ttggaaaaac agcagaaggc tttggcggta
     1021 caagagcaga aaatcaacaa tgagcttgcc cgtttgaaga aaattcaggc aaacgtgcaa
     1081 tccctgctga aaaaacaggg tgtaaccgat gcggcggaac agacggaaag ccgcagacag
     1141 aatgccaaaa tctccaaaga tgcccgaaaa ctgctggaac agaaagggaa cgagcagcag
     1201 ctgaacaagc tcttgagcaa tttggagaag aaaaaggccg aacaccgcat tcaggatgcg
     1261 gaagcaaaaa gaaaattggc tgaagccaga ctggcggcag ccgaaaaagc cagaaaagaa
     1321 gcggcgcagc agaaggctga agcgcgacgt gcggaaatgt ccaacctgac cgccgaagac
     1381 aggaacatcc aagcgccttc ggttatgggt atcggcagtg ccgacggttt cagccgcatg
     1441 cagggacgtt tgaaaaaacc ggttgacggt gtgccgaccg ggcttttcgg gcagaaccgg
     1501 agcggcggcg atgtttggaa aggcgtgttc tattccactg cgcctgcaac ggttgaaagc
     1561 attgcgccgg gaacggtaag ctatgcggac gagttggacg gctacggcaa agtggtcgtg
     1621 atcgatcacg gcgagaacta catcagcatc tatgccggtt tgagcgaaat ttccgccggc
     1681 aagggttata cggtcgcggc aggaagcaaa atcggcacga gcgggtcgct gccggacggg
     1741 gaagaggggc tttacctgca aatacgttat caaggtcagg tgttgaaccc ttcgggctgg
     1801 atacgttgat cggattgccg aaacggcagg ctttatgcaa atcgtgccga tatggtgcgc
     1861 tgttccgtga agtttgccgt ttgccgtacc tgtgttttcc ggtttttgga aatcaagaga
     1921 gaaagttaaa gaagaatgtc gaaacctgtt tttaagaaaa tcgcacttta tactttgggt
     1981 gcaatcagcg gcgtggccgt aagtctggcg gtgcagggtt ttgccgccga gaaggacggg
     2041 cgggataacg aagtcctgcc ggtgcaatcc atccgtacga tggcggaggt ttacggtcag
     2101 attaaggcaa actactatca tgacaaaccc gatgccgatt tgtttgaagg tgcgatgaag
     2161 ggtatggtgg ccggtttgga tccgcattcc gaatatatgg ataaaaaagg ttatgccgag
     2221 ataaaggagt ccaccagcgg cgaatttggc ggcttgggga tggaaatcgg gcaggaagac
     2281 ggttttgtca aagtggtttc gccgattgag gacacgcctg ccgaacgggc ggaggtgaaa
     2341 agcggcgatt tcattgtgaa aatcgataat gtttcgacgc gcggtatgac ggtcagcgaa
     2401 gcggtgaaaa aaatgcgggg caagccgggt acgaagatta ctttgacgct gtcgcgcaaa
     2461 aatgccgaca agccgatagt cgtcaacctg acccgtgcca ttattaaagt gaaaagcgtc
     2521 cgccatcacc tgatcgaacc cgattacggc tatatccgcg tgtcgcagtt ccaagagcgg
     2581 acggtcgaaa gcgtcaatac cgccgcaaaa gagctggtaa aggaaaataa aggaaaaccg
     2641 ctcaaggggc tggtgttgga tttgcgcgac gaccccggcg ggcttttgac cggcgcggtc
     2701 ggcgtgtcgg cggcgtttct gccgtctgaa gcggtcgtcg tcagcaccaa gggacgcgac
     2761 ggcaaagacg gcatggtact gaaagccgtt cccgaggatt atgtgtacgg tatgggcggc
     2821 gatcctttgg cgggtattcc tgccgagttg aaaacgattc cgatggcggt attggtcaat
     2881 tccggttcgg cttccgcgtc ggagattgtc gccggcgcat tgcaggacca caaacgcgcg
     2941 gtcatcgtcg gtacgcagag cttcggtaaa ggttcggttc agactttgat tcctttgtcc
     3001 aacggcagcg cggtcaagtt gacgaccgcc ctgtattaca cgccgaacga ccgttccatt
     3061 caggcacagg ggattgttcc cgatgtcgaa gtaaaagata aggaacgtac ttttgaaagc
     3121 cgcgaggcgg acctggtcgg acacatcggc aatcccttgg gcggcgagga tgtgaacagt
     3181 gaaacccttg ccgtaccgct tgaaaaagat gcggataagc ccgctgcaaa agaaaaaggt
     3241 aaaaagaaaa aggacgagga tttgtcttca aggcggattc cgaaccctgc gaaagacgat
     3301 cagttgcgta aggctttgga tttggtcaag tcgcccgagc agtggcagaa gtctttgggg
     3361 ctggcggcga aaaagccggt ttcaaataaa gataaaaaag ataagaagta ggttttgacg
     3421 atgccgtctg aaagggtttc agacggcata ttatgtttga aaggacaggt tatgagcgat
     3481 tttttaccgg aatttgaacc gtgcggc
//
LOCUS       DAXDXW010000117         3231 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXDXW010000117 DAXDXW010000000
VERSION     DAXDXW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.118"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(211..612)
                     /locus_tag="ACFB64_002021"
     CDS             complement(211..612)
                     /locus_tag="ACFB64_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFC1210305.1"
                     /translation="MFIPAALHKDEHSAYGVTIPDLPGCFSCGDTVEEAVANARSAAY
                     MHIDGMIEDGGFKNLAVSSIADLSQEPDYHGATWVMIEIDPAKISRQQIRFNVSWPQY
                     LLDRVDEYTSANHETRSGFLAKAALLTMNQA"
     gene            complement(714..896)
                     /locus_tag="ACFB64_002022"
     CDS             complement(714..896)
                     /locus_tag="ACFB64_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFC1210306.1"
                     /translation="MNSLDVIALLKQDGWYKVAQSGSHSQYKHPTKKGRVTVPHPKKD
                     LPTGTVKNIYKQAGLK"
     gene            complement(1066..1884)
                     /locus_tag="ACFB64_002023"
     CDS             complement(1066..1884)
                     /locus_tag="ACFB64_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HFC1210307.1"
                     /translation="MNQYAMRFAVIRFMPYVQTREFANIGIIITHPKSGYFDFKIEQR
                     YSRLSRFFRRFDSSVYKAATRAFAEELQRIGKLAAYSAPDQIRAMLDHLTRPREALIM
                     AAQPGVTLAPDRGQELNRLFDYFVARSFAKSQPEAELTRQIQAMLKPLQTAYPFKEST
                     IGDPSGFHASIPLVQKAENGEIRKIIKPIYFGQKDPADIYYKSDKWIAGIKRLRRGGY
                     IDRSEILFAYEPPERPDKAQEKALLDVLGDLEEQGIRLADNRSEGKIIRNFACG"
     gene            complement(2157..2912)
                     /locus_tag="ACFB64_002024"
     CDS             complement(2157..2912)
                     /locus_tag="ACFB64_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFC1210308.1"
                     /translation="MKKRELNEIETAECAELKRIFNSKKEELKLTQYKLAEAVGVTQS
                     AVNHYLNGTNALNASIASQFAKILQIPVSDFSLRLAEEISSMSIGIDGDKLLALQADN
                     LDTNTITLNLYDVSASCGHGVVNPDYPQLLRSIEIPNDALFELLGTNNLTNVQLMPPD
                     GDSMEPTIPQKSITLIKTDVSKFQTGGIYLFTFDGYTYIKRLSRGKGGAIHATSDNRH
                     YAKSDFLINPEEADKFHIHGKFWKVLPLDFLDL"
     gene            3049..>3231
                     /locus_tag="ACFB64_002025"
     CDS             3049..>3231
                     /locus_tag="ACFB64_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000437878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFC1210309.1"
                     /translation="MKKIPLNEYVEQHGQAKTAKEIGVTQGAISKALRSGRAIFLFSD
                     GKRVKAEEVRAFPSTRL"
BASE COUNT          736 a          691 c          848 g          956 t
ORIGIN      
        1 attaccggca gtaatttatt attgcaatac cgctagtaat attatttacg gtaatttata
       61 tttatctttg attattaaaa gaatttattt ttgaaattcc gcaggcgcaa aaaaaccgcc
      121 tattaaggcg gctttgtcgg ttttgtgttg ttttcaggtt cggcggggcg tgaaaaaagc
      181 ccgcgtgtgc gggcgggttg aacgggctgg ctatgcctga ttcatggtga gcagggcggc
      241 tttggctaaa aaaccgctac gggtttcatg atttgcggat gtatattcat ccactctatc
      301 aagaaggtat tgcggccagc tgacgttgaa tcggatttgc tgccgactga tttttgcagg
      361 gtcgatttca atcattaccc atgttgcccc atgataatca ggttcttggc tcaaatcggc
      421 aatgctgctt acggcaaggt ttttgaaccc tccgtcctca atcataccgt caatatgcat
      481 ataggcggca gaacgggcgt tggcgacggc ttcttcaacg gtgtcgccgc aagagaaaca
      541 gccgggaagg tcggggatgg ttacgccata tgccgaatgt tcgtctttgt gcagggcggc
      601 agggataaac atttgatttc ctttaattat gttgttcagg tttatgggcg cgtgagataa
      661 cccgtcgatt gtttgattag aaggggggcg gcgggaaacc ccgctgcctg ccgttacttc
      721 aaaccggctt gcttatagat attttttaca gtacctgtcg gcaagtcttt tttcgggtgc
      781 ggcacggtta cacggccttt ttttgttggg tgcttatatt gcgaatggct cccggattgt
      841 gcaactttat accaaccgtc ttgtttgagt agggcaataa cgtctaggct attcaattat
      901 cactcctaat cttaatgcat ggcacgcctg ccaatgtgcg taataataca cactaatttt
      961 tattttgtca atttttatgt gtatttgtgt gtaaaaatat ctttcggcag tagttgcagg
     1021 gaagggtgta aaaagcccgc ataacgcggg cggtcttgag ggctgtcagc cgcaggcaaa
     1081 gtttctgatg attttgcctt cgcttcggtt atcggcaagc cgtatgccct gttcctccag
     1141 gtcgcccaat acgtcaagca atgccttttc ttgtgccttg tcgggacgct ccggcggttc
     1201 gtaggcaaac aatatttcag agcggtcgat gtatccgccg cgccgcaacc gttttatgcc
     1261 ggcaatccac ttatcagact tgtaatagat gtcggcaggg tctttctgtc cgaaatagat
     1321 aggcttgatg attttccgta tttcgccgtt ttccgccttt tgcaccaagg ggatggaggc
     1381 gtgaaagcct gacgggtcgc cgattgtgct ttctttgaag gggtatgccg tctgaagcgg
     1441 ctttaacatt gcctgtattt gtcgggtaag ttctgcttcg ggttggcttt tagcaaagct
     1501 gcgggcgaca aaataatcaa acaggcggtt cagttcctgc cccctgtcgg gggcgagggt
     1561 tacgcccggt tgggcggcca taatcagggc ttcgcgcggg cgggtcaaat ggtcgagcat
     1621 ggcgcgtatc tgatcgggcg cggagtatgc cgccaatttc ccaatccgct gtaattcttc
     1681 tgcaaaggca cgggtcgccg ctttatagac ggacgaatcg aagcggcgga aaaaacggct
     1741 caatctgctg taacgctgtt cgattttaaa gtcgaagtag ccgcttttgg gatgggttat
     1801 gatgatgccg atgttggcaa attcgcgcgt ttggacatag ggcataaacc gtatgacggc
     1861 aaaacgcatg gcatattggt tcatgagatg ctccaaagtg ttccgttatc aatgcggcgg
     1921 acggtttcgt cggtgcggct gcggttatag gcggcgggca ggtcgcgctc ttcgtttgcc
     1981 cgtgcccgct ccggcggcag attatcacac gcttttcttt tctttaccct atgaaattta
     2041 cactttgtgt aaatctgtaa atccgacttt ttgagatttc ccgggaggaa tttgtcaatc
     2101 cggcacgccc tgccggaaga ccgggccgat gacggtcagg ctgtccaaag gggcggtcaa
     2161 aggtctaaaa aatcaagcgg caaaactttc caaaatttgc cgtgaatgtg gaatttgtcg
     2221 gcttcttcag ggtttatcag aaaatccgac ttggcgtaat gcctgttgtc gctggtagcg
     2281 tgtatcgcgc cgcctttgcc tctggacagg cgtttgatgt aggtataacc gtcaaaggta
     2341 aacaggtaga tgccgccggt ctgaaacttg ctaacgtctg ttttgattaa agttatcgat
     2401 ttctgtggaa tggtcggctc catgctgtcg ccgtccggcg gcataagctg cacgtttgtc
     2461 agattgtttg tccccaaaag ctcgaatagc gcgtcattgg gaatctcaat cgagcgtagc
     2521 agttgcggat agtcggggtt gactacccca tgaccgcaac tggctgatac gtcgtatagg
     2581 ttgagggtga tggtgttggt gtcgagattg tctgcttgca gtgccagcaa tttatctcca
     2641 tcaatgccga tggacatact gcttatttct tcggcaagtc ggaggctgaa atccgaaaca
     2701 ggaatttgca agattttggc aaactggctt gcaatggagg cgtttaaggc gttggtgcca
     2761 tttaaataat ggttcaccgc gctttgagta actcctacag cttctgccag cttgtattgg
     2821 gttagcttaa gttcttcttt ttttgagtta aaaattcttt ttagttcggc acattcagcc
     2881 gtctcaattt catttaattc tctttttttc attttgccgc ctccttcgat aatttaaaca
     2941 tattaaataa tttaaatata ttactgtaag taataataat caaataaccg caagtattga
     3001 cttttgaatt acttgcggta ataatatctc tattatttgg agtaatttat gaagaaaatt
     3061 ccgctaaatg agtatgttga gcagcacgga caggcgaaaa cagcaaaaga aatcggcgtt
     3121 actcaaggag caatttcaaa agcattgagg tctgggcggg caattttttt attttcagac
     3181 ggcaaaagag tgaaggctga ggaagtgcgg gctttcccct cgacacgtct g
//
LOCUS       DAXDXW010000118         3099 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXDXW010000118 DAXDXW010000000
VERSION     DAXDXW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.119"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            112..2369
                     /locus_tag="ACFB64_002026"
                     /pseudo
     CDS             112..2369
                     /locus_tag="ACFB64_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
     gene            2382..3024
                     /gene="pgmB"
                     /locus_tag="ACFB64_002027"
                     /pseudo
     CDS             2382..3024
                     /gene="pgmB"
                     /locus_tag="ACFB64_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
BASE COUNT          780 a          930 c          786 g          603 t
ORIGIN      
        1 ccttgccgta ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
       61 cactataaga tttcaccatt ccctcaaatc aatccaaaca ggagctttac gatgtacaca
      121 agaattatgg aaatcagccc ttggacgctg cgttcggcaa aactggaaaa agaacacaaa
      181 cggctgcaag agagcctgac cagcttgggc aacggctata tgggtatgcg cggcagcttt
      241 gaggaaacct actccgccga cagccacttg ggcacctaca tcgccggcgt gtggtttccc
      301 gacaaaaccc gcgtcggctg gtggaaaaac ggctatccca aatatttcgg caaagccatc
      361 aacgcgctca atttcagcaa agtcaaaatc tttgtcgacg ggcaagaggt ggacttggcg
      421 aaaaacgacg ttgccggctt ctccgtcgaa ctggatatgc aacacggcgt gttgcgccgc
      481 tcgttcaccg tattcggtgt gcgtttcgat gtgtgcaaat tcctgtcggt cgcgcaaaaa
      541 gagctggctc tcacccgctg ggaagccgtg tccgttgacg gcaaaaccca ccaagtccgc
      601 atcgattcca tcatcgatgc cgacgtgaaa aacgaagact ccaactacga agaaaaattc
      661 tggcaggtat tggacaaagg cgtttcagac gaccgctcct acattgccgc ccaaaccgtc
      721 gccaaccctt tcggtgtgga acaatttatc gtcaacgccg ggcaaacctt cgccggcagc
      781 ttcaaagcct tcggcggcag ccaaaccgat tggcaggtct ctaattcttt tgaagccgaa
      841 gtcggcggca cgcccgaaac ctttgaaaaa cgcgtgattg ttaccaccag ccgcgattat
      901 cagggcttgg aagcagtgaa agccgtcggc cgcgccttat ctgaaaaaat cgcaggtgtt
      961 gcgtttgaaa ccttgctgga tgcgcacaaa gcaggctggc agcatcgttg ggaaatcgcc
     1021 gacgtggtca tcgaaggcag cgacgaagca cagcagggca tccgcttcaa cctgttccaa
     1081 ctgttctcca cctactacgg cgaagacgcg cgtctgaaca tcggccccaa aggctttacc
     1141 ggcgaaaaat acggcggcgc gacctattgg gacaccgaag cctacgccgt gccgctctac
     1201 cttgcactgg cggaacccga agttacccgc aacctgctgc aataccgccg caaccaaatt
     1261 gccgcaggtg cagcataacg cacgcgaaca gggcttggcg ggcgcactct atccgatggt
     1321 aacgtttacg ggcatcgagt gccacaacga atgggaaatc accttcgagg aaatccaccg
     1381 taacggcgcg attccttacg ccatctacaa ctacaccaac tacaccggtg acgagtgcta
     1441 tcttgccaaa gaaggcttgg aagtcttggt cgaagtgtcc cgcttccggg cggaccgcgt
     1501 ccacttctcc aaacgcaacg gcaaatacat gattcaaggc gtaaccggtc cgaacgaata
     1561 cgaaaacaac atcaacaaca actggtaaac caacaccctc gccgcatggg tattgggcta
     1621 cacccgcgaa gccttggcga aatacccgcg tccgggttcg aacgtgagcg ccgccgagtt
     1681 ggaaaaatgg gcggacatca gcgcgaatat gtaccgtccg catgacgaag aactcggcgt
     1741 attcgtgcag cacgacggct tccttgacaa agacatccgc cccgtatccg cgctttcgcc
     1801 cgacgatttg ccgctcaacc agaaatggtc gtgggacaaa atcctgcgtt cgcccttcat
     1861 caaacaggcg gacgtattgc aaggcatcta cttcttcggc gaccgtttcg acatggacga
     1921 aaaacgccgc aacttcgact tctacgaacc gatgaccgtg catgaaagct cgttgtctcc
     1981 atgtatccac tccatcctcg ctgccgaact gggcaaagaa gaaaaagccg tggaaatgta
     2041 ccggcgcacc gcccgcctgg acttggacga ctacaacaac gacaccgaag acggcctgca
     2101 tattacttct atgaccggct cgtggctcgc catcgtccaa ggtttcgccc aaatgaaaac
     2161 ttggggcggc aaactcagct tcgcaccgtt cctgccgagt gcgtggacag gctatgcctt
     2221 ccacatcaac taccgcggcc gtctgattaa agtcgccgtc ggcaaaaaac gtcgtcttca
     2281 ccctgctcaa aggcgatccg ctcgaattgc aggtgtacgg caaagatatc acgctcaacg
     2341 gcagccacac cgttgcattg gaaaaataag gagggcgcaa aatgactttt accgcagtgc
     2401 tcttcgatct cgacggcgtg attaccgata ccgccgaata ccactacctc gcatggaaaa
     2461 agcttgccga agaattgggc atcggcatcg accgcaagtt taacgagcag ctcaaaggcg
     2521 tgtcgcgcga cgattcgctc aaacgcatcc tcgcgcacgg cggcaaaacc gtcggcgaaa
     2581 ccgagttcgc cgaactgacc tgccgcaaaa acgacaacta cttagagatg attcaggcag
     2641 tcaaacccga agacgtgtac cccggcattt tgccgctgct ggaagcattg agggcaaacg
     2701 gcaaaaaaat cgccctcgcg tccgccagta aaaacggccc gttcttgctc gaacgcatgg
     2761 ggctgaccca cttcttcgac accgtcgccg accctgccgc cgtcgcgcat tccaaaccct
     2821 cccccgacat cttccttgcc gccgccgagg gcgtgggtgc ggacatacgc cgctgccggc
     2881 gtcgccgcca tcaaagcctc tggcgctttg cccatcggcg tgggcaaaac cgaagacttg
     2941 ggcagcgaca tcgcgctggt gtccgacacc gccgagctga cctacgccta cttgcaaaat
     3001 gtgtgggaac agtcgggcag ataaaattct aaggaagtaa aaaggccgtc tgaacagtgt
     3061 ttcagacggc ctttctgctt ttagaacaga atgataacc
//
LOCUS       DAXDXW010000119         3092 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXDXW010000119 DAXDXW010000000
VERSION     DAXDXW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.120"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            107..322
                     /gene="rpmE"
                     /locus_tag="ACFB64_002028"
     CDS             107..322
                     /gene="rpmE"
                     /locus_tag="ACFB64_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFC1210310.1"
                     /translation="MKQGIHPNYREVNVTCSCGNKFVTKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
     gene            586..1214
                     /locus_tag="ACFB64_002029"
                     /pseudo
     CDS             586..1214
                     /locus_tag="ACFB64_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
     gene            1360..3036
                     /locus_tag="ACFB64_002030"
     CDS             1360..3036
                     /locus_tag="ACFB64_002030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HFC1210311.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVIGLTSCGFAGFNFLGRHHGRSVVLIHIGCIGLIPVAHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLNYHVFGTFGGVRHIQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGIVWMLAVLVLLAFNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASFSPELKRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDV
                     RCRYRIVRLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
BASE COUNT          660 a          795 c          773 g          864 t
ORIGIN      
        1 ttttatcgaa tatcctcttg acgaaacaac agaaaatagc gagaatgccg agcttgtctg
       61 aggcataggt acatccccta tgcccgcttt tttgaaagga aatcaaatga aacaaggtat
      121 tcacccgaac taccgcgaag ttaacgttac ctgctcttgc ggcaacaaat tcgtaaccaa
      181 atccgcaatg gaaaaagaaa acttcaacat cgaggtttgc tccctgtgcc acccgttcta
      241 taccggcacc caaaaaatcg tcgataccac cggccgcgtg gacaaattca acaacaaatt
      301 cggcaacctg ttcaaacgct gattgccgct ttgcaagaat taagccctgc catcatttcg
      361 atggcagggc tttttaatat ttggcacact gacttcttat ctatatagtg gattaacaaa
      421 aatcaggaca aggcgacgaa gccgcagacc gtacaaatag tacggcaagg cgaggcaacg
      481 ccgtaccggt tttgttaatc cactatattt tgacggggtt gaagttgaca tctacaaagt
      541 ttaatgtttg aatacattaa aaaatatatt tgaatgggat gttttatgat tcaaaatgtt
      601 gttacttcaa taatcctgta ttctgggaca gccgtagact tacttattat cctaatgtta
      661 ttttttgcca aaaaaaaagc ggaaaagaca tcattaacat ctatttagga caatttctag
      721 gctctgttag cctgatattg ctaagtttgc tttttgcatt tgtcttagat tatattccta
      781 gtaaagagat tttaggtttg ctcggcttga ttccgatttt cctaggcctc aaagttttgc
      841 tgttaggaga ttccgatgga gagtctattg ccaaagaggg tttgcgcaaa gataataaaa
      901 acctgatttt tctagtcgct atgattactt ttgcaagttg tggcgctgac aatattggtg
      961 tctttgtccc atattttact accttaaatt tagcgaattt gatagtggct ttacttacct
     1021 ttctagttat gatttatctc ttggtttttt ctgcccaaaa attggcacaa gtcccttctg
     1081 tcggagaaac tttggaaaaa tatagcagat ggtttgttgc cgttgtttat ttaggattgg
     1141 ggatatatat cctgattgaa aacaacagtt ttgatatgct atggactgtg tcgggccagg
     1201 aaaaaatatt atgaaaaaga tagtatctgc aaagactgcc ccgtgcagtc ttttttgttg
     1261 ccggtctttt tgtgtctgat gccgtctgaa gcagtctctg cacgaccttt gtgcgaatat
     1321 ttgctacact tggcaatccg tccatttctt ctcacgctta tgctgaccta taccccgccc
     1381 gatgcccgcc cgcccgccaa aacccacgaa aaaccgtggc tgctgctgtt gatggcgttt
     1441 gcctggctgt ggcccggcgt gttttcccac gatttgtgga atcctgccga acctgccgtc
     1501 tataccgccg tcgaagcact ggcaggcagc cccaccccct tggttgccca tctgttcggt
     1561 caaaccgatt tcggcatacc gcccgtgtat ctttgggttg ccgccgcatt caaacatttg
     1621 ctgtcgccgt gggcagccga cccgtatgat gccgcacgct ttgcaggcgt attttttgcc
     1681 gttatcggac tgacttcttg cggctttgcc ggtttcaact ttttgggcag acaccacggg
     1741 cgcagcgttg ttttaatcca tatcggctgt atcgggctga ttccggttgc ccatttcctc
     1801 aatcccgccg ccgccgcctt tgccgccgcc ggactggtgc tgcacggcta ctcgctggca
     1861 cgccggcgcg tgattgccgc ctctttcctg ctcggtacgg gttggacgtt gatgtcgctg
     1921 gcggcagctt atccggcggc gtttgcgctg atgctgcccc tgcccgtgct gatgtttttc
     1981 cgtccgtggc aaagcaggcg tttgatgttg acggcagtcg cctcgcttgc ctttgccctg
     2041 ccgcttatga ccgtttaccc gctgctcttg gcaaaaacgc agcccgcgct gtttgcgcaa
     2101 tggctcaact atcacgtttt cggtacgttc ggcggcgtgc ggcacattca gacggcattc
     2161 agtttgtttt actatctgaa aaatctgctt tggttcgcac tgcccgcgct gccgctggcg
     2221 gtttggacgg tttgccgcac acgcctgttt tcgaccgact gggggatttt gggcattgtc
     2281 tggatgcttg ccgttttggt gctgctcgcc tttaatccgc agcgttttca agacaacctc
     2341 gtctggctgc tgccgccgct tgccctgttc ggcgcggcgc aactggacag cctgaggcgc
     2401 ggcgcggcgg cttttgtcaa ctggttcggc attatggcgt tcgggctgtt tgccgtgttc
     2461 ctgtggacgg gctttttcgc catgaattac ggctggcccg ccaagcttgc cgaacgcgcc
     2521 gcctacttca gcccgtatta cgttcccgac atcgatccca ttccgatggc ggttgccgta
     2581 ctgttcacac ccttgtggct gtgggcgatt acccggaaaa acatacgcgg caggcaggcg
     2641 gttaccaact gggcggcagg cgttaccctg acctgggctt tgctgatgac gctgttcctg
     2701 ccgtggctgg acgcggcgaa aagccacgcg cccgtcgtcc ggagtatgga ggcatcgttt
     2761 tccccggaat taaaacggga gctttcagac ggcatcgagt gtatcggcat aggcggcggc
     2821 gacctgcaca cgcggattgt ttggacgcag tacggcacat tgccgcaccg cgtcggcgat
     2881 gtccgttgcc gctaccgtat cgtccgcctg ccccaaaacg cggatgcgcc gcaaggctgg
     2941 cagacggtct ggcagggtgc gcgcccgcgc aacaaagaca gtaagtttgc actgatacgg
     3001 aaaatcgggg aaaatatatt aaaaacaaca gattgagccg aatttctgga ttaagtgccg
     3061 gaaatcgcgt ataattgcgc gattaaaccc tt
//
LOCUS       DAXDXW010000120         3050 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXDXW010000120 DAXDXW010000000
VERSION     DAXDXW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.121"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(110..301)
                     /locus_tag="ACFB64_002031"
     CDS             complement(110..301)
                     /locus_tag="ACFB64_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210312.1"
                     /translation="MLPACSVSSLLLGEYLEFEENGTKIAAEIGSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            complement(321..869)
                     /locus_tag="ACFB64_002032"
     CDS             complement(321..869)
                     /locus_tag="ACFB64_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210313.1"
                     /translation="MEKIINSKVRETNFNQAWRVIRAMQINNISDEQVMIVKMSCYLK
                     KDEIFKNIERYGYQSSDIKKINPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKPGETLYLNFKSIKNQCKNYFFAVLVLMPLTLKAVR
                     VKWQIIILFLMPFLYSLFNQII"
     gene            complement(857..1195)
                     /locus_tag="ACFB64_002033"
     CDS             complement(857..1195)
                     /locus_tag="ACFB64_002033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210314.1"
                     /translation="MKTFEKTWSAQYRDMEISVRNFWNLKRTGAEVYINGRRVYHNEA
                     GMASASLRSLMGEYLEFEESGTKITVEIGSAWHFNETIRNINTHLRGTKLPGSNWRVQ
                     DKGHFHLWKR"
     gene            complement(1240..1617)
                     /locus_tag="ACFB64_002034"
     CDS             complement(1240..1617)
                     /locus_tag="ACFB64_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210315.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRESFIFSDVLLQKGESEENFSEIVLKSIKNN
                     KILTVEVRSGLHFDLRNIYRIEM"
     gene            complement(1675..2040)
                     /locus_tag="ACFB64_002035"
     CDS             complement(1675..2040)
                     /locus_tag="ACFB64_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210316.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMPSDDLFLKCLQSSYKAYSETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(2022..2561)
                     /locus_tag="ACFB64_002036"
     CDS             complement(2022..2561)
                     /locus_tag="ACFB64_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210317.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAGGLGAIPGDV
                     GAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQGI
                     GRHRPWESKSTDRSWKNRF"
     gene            complement(2604..>3050)
                     /locus_tag="ACFB64_002037"
     CDS             complement(2604..>3050)
                     /locus_tag="ACFB64_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC1210318.1"
                     /translation="PALIAFLESTVYEKSRNKASSGKSWLIGATTYKAIDPIVLSLTA
                     AYRINGSKTLSDDVKYKAGNYWMLNPNISFAANDRISLTGGIQWLGKQPDRIDGKKES
                     ARNTSTYAHFGAGFGFTKTAALNASARFNVSGQSSSELKLGVQHTF"
BASE COUNT          917 a          672 c          522 g          939 t
ORIGIN      
        1 ataaagaaac ggcaacacac gccaacagaa aaacatattt gaacttcatc atattttcca
       61 cataaaaggc agcctgaaaa tctttcaggc tgcccttgtc aaattattcc tagctttcgg
      121 cttttttggc aaaccaaaca atccgattac ccgcataata ctttccattt attgaaatcc
      181 gacaagccgc gcccaaaaaa tgccatgcac tgccgatttc cgcagcaatc tttgtaccgt
      241 tttcttcaaa ttccaaatat tcacccaata ataaacttga aacagaacac gcgggcagca
      301 agtgcccacc ctacgcttat tcaaataatt tgattaaata aagaataaag aaaaggcatc
      361 aggaacaaaa ttataatctg ccacttgact cgcaccgctt ttaaagtaag gggcatcaaa
      421 accaaaaccg caaaaaaata atttttgcat tgatttttaa tagatttaaa attcaaatat
      481 agtgtttctc caggtttaaa aagtttatct ttatctatat atttgatgct ttccctatcc
      541 aaaataaata tttcaaacat taaaaaatca ttacatgacc aagccaaatc ataaatttga
      601 ttcaaatggg tatcataaag ataaaaataa taaggtttgg gaacaggtaa aatatttaat
      661 ggaaaaggag gattaatttt cttaatatct gatgactgat atccatatct ttctatattc
      721 ttaaaaattt catctttctt aagataacaa gacattttga caatcataac ctgttcatca
      781 gatatattat ttatttgcat ggcgcgtata acacgccatg cctgattaaa attagtctcc
      841 cttaccttag aatttattat cttttccaaa gatgaaaatg ccccttatct tgaactctcc
      901 agttagaacc tggaagtttc gtacctctta aatgtgtatt aatatttctt atagtttcat
      961 taaaatgcca cgcgctgccg atttcaacgg taattttcgt accgctttct tcaaattcca
     1021 ggtattcccc cattagcgaa cgcaaagaag cagacgccat cccggcttcg ttatgataaa
     1081 cccgcctgcc gttgatatag acttccgccc ctgtccgttt caaattccaa aaattccgta
     1141 ctgaaatttc catatcccga tattgtgcag accatgtttt ttcgaaggtt ttcataaaat
     1201 ttcctatacc tgtccaatcg gcacatatca attgcattat tacatctcaa tacgataaat
     1261 atttcttaag tcaaaatgca agcctgaccg tacctcaact gtcaaaattt tattattttt
     1321 tattgatttt aagacaattt ctgaaaaatt ctcttcgctt tctccctttt gtagaagcac
     1381 atcagaaaaa ataaaacttt cccgattaaa ttcataaata tgtttcaacc attcacctcc
     1441 tctttctgta aggcaagatt cagtttcatt cttccttatt gtataaatat ttccttcaca
     1501 aaatctgaaa taaatccata aatccatctt atccataatt aaagaaaaag tttcacctcg
     1561 agattttgtc aacaattcgc aaggttgcga tgttgcaatc aaatagccga aagacatttt
     1621 ttacctcata catggtcgaa atcagtttct gttagttcag aatccatttt ttcgtcaaca
     1681 actgaatccg catttttgaa ttaacgtttt catcagctgc cgtttatcta aaccggcagg
     1741 ttcagtttca gaataagcct tatatgaaga ctgtaagcat ttcagaaaaa gatcatcaga
     1801 aggcatatct gccgaatcaa atacaactgt tttgattttg gtacttaccc aaaacccttt
     1861 ttgctctttt tctactatac ggaaattcag aatatttcca accgaatcaa aagcacggta
     1921 aacatcatcc atcaaatcct gcggctctat tttcttttcc aattccgaca atccttgaaa
     1981 tatatccaaa gacacatctt caaatagaaa aaaaggagga gttagaagcg gtttttccat
     2041 gatctgtccg tagattttga ttcccaaggg cgatgacgac caattccctg tccaggcaaa
     2101 gtcttgcccg tattatccgt aactcgacga tgataatggg gaaattttcc aataggatga
     2161 cctgttctat taccgaaagg ggctatccgc atattattgc cgattttaat ctcacgtcca
     2221 tatttagcaa aggaaacaac ctttcctgca gcacctacat caccaggaat tgcgcctaat
     2281 ccgccagcaa catctctaac agaagctggt ctgcctgtcg ttgcataact aaaaccatgc
     2341 tgtgtccaca taccaatggc agcaccaccc aagatagcca atggaagaaa agccccttca
     2401 gtctccttca tctccttctg agaaagctcc gccaactgca tcggcgcatc tgcccgcgtg
     2461 tggaacactt ggtcttcaaa tgcctgattg tccaatccgt ttgccattgc gggggcgatc
     2521 atagacagca tcattacggc tgcggtgatt tgttttttca taataactcc tttggattac
     2581 aaggttggaa aatcaaaatc tgcttaaaat gtatgctgta cgcccaattt cagttcggaa
     2641 ctgctttgcc ctgaaacgtt gaaacgtgcg gatgcgttta aagccgcggt tttggtgaaa
     2701 ccgaaacctg cgccgaaatg ggcgtaggtg gatgtgtttc ttgcggattc ttttttgccg
     2761 tctatgcggt cgggctgttt gcccagccat tggatgcctc cggtcaggct gattctgtcg
     2821 ttggcggcaa atgagatgtt gggattcagc atccagtaat tgcctgcttt gtatttgacg
     2881 tcgtctgaaa gggttttgct gccgttgatg cggtaggcgg cggtgaggga aaggacaatc
     2941 ggatctatgg ctttgtaggt ggtggcgccg atgagccacg attttcccga cgaggctttg
     3001 ttgcgcgatt tttcgtaaac cgtgctttcg aggaaagcga tgagtgcggg
//
LOCUS       DAXDXW010000121         2956 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXDXW010000121 DAXDXW010000000
VERSION     DAXDXW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.122"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..223)
                     /locus_tag="ACFB64_002038"
     CDS             complement(<1..223)
                     /locus_tag="ACFB64_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC1210319.1"
                     /translation="MEQKRRFAASLLLAAALPLCAHSFPFAEENPIAYGKVKIQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQ"
     gene            complement(279..503)
                     /locus_tag="ACFB64_002039"
     CDS             complement(279..503)
                     /locus_tag="ACFB64_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210320.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQGSIGKTEEPSYFLMFMFL
                     NSLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            complement(928..1214)
                     /locus_tag="ACFB64_002040"
                     /pseudo
     CDS             complement(928..1214)
                     /locus_tag="ACFB64_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1450..1722)
                     /locus_tag="ACFB64_002041"
     CDS             complement(1450..1722)
                     /locus_tag="ACFB64_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210321.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIS
                     YVLIKNILVSVSDGGGIKYVAIYLSNLFFWTVLMYALVKRLSKKPS"
     gene            complement(2599..2847)
                     /locus_tag="ACFB64_002042"
     CDS             complement(2599..2847)
                     /locus_tag="ACFB64_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210322.1"
                     /translation="MNIKNEIVALISKKIDGGQYSSLENFALSVLREKIINNTVSSDE
                     KLLIINDFLSGFLEFDPETGEPVGETLKIEQMIDFFLV"
BASE COUNT          938 a          595 c          478 g          945 t
ORIGIN      
        1 tttgcccgta aaaattgttc aaaagtgtcg ccaccgaagc cgccccgcag gaaaaatcca
       61 aatcctgctt tacaatattg aaatcccgcc gcgctttcca actctgtatt ttgactttgc
      121 cgtaggctat ggggttttct tccgcaaagg gaaaagaatg tgcacaaagc ggcagggcgg
      181 cggcgaggag cagggaggcg gcaaaccgcc ttttttgttc cattaaaatc ctcctgaaag
      241 gctgcctgaa atttccaagc agcctttttg tttgcttatt aatcggatag tgcgacaaat
      301 ataaagtggg cggcaatcat tgccgtaacg gcagccataa ctgttttatt ttcttcaaac
      361 caaagcgagt tgagaaacat gaacatcaag aaatatgatg gttcttcagt tttgccgatg
      421 cttccttgag tcagaagata aaatatggat atggcggtta tgccgattgc tgcttttatt
      481 aaaatatatt tgttcttcat catcttgtat ggtttttaat gcaagtttca acaataaaat
      541 tcggaataag atagagagat gttgatatat cggataaata tatagtggat taaatttaaa
      601 ccggtacggc attacctcgc cttagctcaa agagaacgat tctctaaggt gctgaagcac
      661 cgagtgaatc ggttccgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt
      721 aaatttactc cactatacaa aacaatcaag aaacgattgg cggtatgtat aatcttgaat
      781 tttgtttttt gatgaaatta aaataatgaa atatatggta aacgttacaa cggaagcctt
      841 agcctcccga ttgcagtatt tcaattccaa gatggatgcc gcatagaaga taaaatactc
      901 aaatggtttt ttagcggctt aaatatctta tttaatctta ttgattgttt ttccaatggt
      961 ttctgcaatc aataataaga aatagaataa taaacaaaca gaacatgaaa ataaaaataa
     1021 atctttcatt atcccagtaa taaaattcca aagattttcc ttatttccat caacaataaa
     1081 aattatcagt tcatgaataa agaatactgc aaagaaaatg cttcctgccg ttagggatat
     1141 ttctaacctg cttattttcc cttcactgta tttacctgat aaatagccta aaaagctgca
     1201 atcaaatcat ctattttaaa aatttctgta aaaacaggta atgcctttaa ttcattacct
     1261 gttttgccat tatttatcta tgatataagc gtctcttacc cttcagcctc tttgggaaat
     1321 caaagcctgc ttagaacgta tgttgcacac ccaatttcaa taggtaaatc agattgcaaa
     1381 tccagcaatt tgaatattgt cattgttccg tgcaaaagga atctttattg atgtgttgtg
     1441 tactgggttt cagcttggct ttttagataa tcttttcaca agagcataca tcaatactgt
     1501 ccaaaaaaaa agatttgata aatatattgc aacatatttt attccacctc catccgaaac
     1561 agaaaccaat atatttttaa ttaatacata gctgattatt acatttatta ttcctataat
     1621 atataaggat cttgccaaaa ttttttttga ttttatttta ggaatatctc ttatccatgc
     1681 taaaatacag cccaatgtcg aaaagaaaat aacagcaaac actaccttac ctcctctcac
     1741 ttccaaccaa accgatagca ggtttggttg gaagttggtt tatttattat ttaacggcga
     1801 atgtcagtgt tcttacccgt agaacctgca taaccagtta tgccaccagc aacagccccc
     1861 actgctacac ctcgcatacc attaatcaga attcttccta ttttttcgaa tccactattt
     1921 cttccaaaga tgcagcaaac gcaggttgag cctagaccca atctacgttt gctgccgcat
     1981 ctgatcccta ttgtttctta tccttacatc ttcctgcctt gtcaatcaaa taaagacaga
     2041 agactataca aaaactgacc gacatactga aaatacctat ccccttccat gcaatctccg
     2101 caccattgac ccaatagatc agactgagaa acaacaaagc cacagtataa atcaatgtaa
     2161 aaaatatagc gtaaagcgca ataaagggaa cttttatctc acggttgttt tttataatat
     2221 attcagcaac ttgatttccg aatatacctg ataaaaaata tagtattaga tcatccattt
     2281 cgtttatctt ctatgttttc ccattgcggc gctagaagac tgatttaatg catgcaccat
     2341 gcaatatagt ggattaacaa aaaccggtac ggcgttgccc cgccccggct caaagggaac
     2401 gattccctaa ggtgctgaag caccgagtga atcggttccg tactatctgt actgtctgcg
     2461 gcttcgttgc cttgtcctga tttttgttaa tccactatag tttaaacaac tttatttttg
     2521 attttatccc aagtataaac ctgacaatcg tatttaaaag ccggatctac gcctagcttg
     2581 tccactggta tgtatgattt agactaaaaa gaaatcaatc atttgctcaa ttttaagcgt
     2641 ctccccgaca ggttcccctg tctcaggatc aaactcaaga aaacctgata aaaaatcatt
     2701 aataatcaat aatttttcat cactggaaac agtattgttt ataatttttt cccttagtac
     2761 ggataaagca aaattttcta aagaactata ttgcccccca tcaatttttt tagaaattaa
     2821 agcaactatt tcatttttaa tattcataat ttatccttaa gttataaaag gggcaaagcc
     2881 cccttttttt gattaacgag gctttgttgc gcgatttttc gtaaaccgtg ctttcaagaa
     2941 agctgattag ggcggg
//
LOCUS       DAXDXW010000122         2869 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXDXW010000122 DAXDXW010000000
VERSION     DAXDXW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.123"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..420
                     /locus_tag="ACFB64_002043"
     CDS             <1..420
                     /locus_tag="ACFB64_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC1210323.1"
                     /translation="ADWHTYFVKGDKAETEGVWVHNDCPTKLKPTERYNRQTHYGGSQ
                     TDGARAQAARQAGEGKPCPTCGRIQIFGTKTAPSPQHEPPLVKHYYEHGGHSMSNADR
                     AKHARESIKGTQCLTCQRKEGAMMSRYSREQAKKHGL"
     gene            410..958
                     /locus_tag="ACFB64_002044"
     CDS             410..958
                     /locus_tag="ACFB64_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC1210324.1"
                     /translation="MDYNQTVLSHLQKFWKHHDIKGFTWTLGRIVEELPDFQVFQVIP
                     NHEDEPWVYVSSGIGQFLGQEFFIISPFETPEHIETLAMLASASMHYPDQFQLGKTVN
                     IGRPWVEQSSFRHFLISLPYPYGQELEYMDNVRFFWLLPITQTERLFLNTHSVEELET
                     KFDEAGIDYLDINRASTVWQAG"
     gene            1255..1608
                     /locus_tag="ACFB64_002045"
     CDS             1255..1608
                     /locus_tag="ACFB64_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210325.1"
                     /translation="MGIKNEQIIICKKYNTEIYPVSDVSKIGVAENVKQTGLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            1598..1921
                     /locus_tag="ACFB64_002046"
     CDS             1598..1921
                     /locus_tag="ACFB64_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFC1210326.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     QLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            1980..2207
                     /locus_tag="ACFB64_002047"
     CDS             1980..2207
                     /locus_tag="ACFB64_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210327.1"
                     /translation="MEIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            2334..2869
                     /locus_tag="ACFB64_002048"
                     /pseudo
     CDS             2334..2869
                     /locus_tag="ACFB64_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
BASE COUNT          945 a          488 c          599 g          837 t
ORIGIN      
        1 gccgattggc atacctactt tgtcaaaggc gataaggcgg aaacggaagg ggtttgggtt
       61 cataatgatt gtccaaccaa actaaaaccg acagaacgtt ataatcgtca aacgcattat
      121 ggaggttctc aaacagatgg agcaagggct caggcagcaa gacaggcagg agaaggtaaa
      181 ccttgtccaa cttgtggtag aattcagata tttggtacga aaaccgcacc aagtccgcaa
      241 catgaacccc cattagtaaa acattactat gaacatggtg gtcactctat gagcaatgct
      301 gatcgagcga aacatgccag ggaaagcata aaaggaactc aatgtttaac atgtcaaagg
      361 aaagagggtg cgatgatgtc aagatattcg agagaacagg ctaaaaagca tggactataa
      421 ccaaactgtt ttatctcact tgcaaaaatt ttggaaacat catgatatta aaggatttac
      481 ttggacttta ggaagaattg ttgaagaact tcccgatttc caagttttcc aagtgattcc
      541 taatcatgaa gatgagcctt gggtttatgt cagctctggg ataggtcaat ttttagggca
      601 ggagtttttt atcatcagcc cgtttgaaac acctgaacat attgagacct tggcaatgct
      661 ggcatctgca agtatgcatt atcctgatca atttcaatta gggaaaaccg tcaatatcgg
      721 tagaccgtgg gtcgagcaat catccttccg gcattttctg atttctctgc cttatcctta
      781 tgggcaagag ttggaatata tggataatgt ccgatttttc tggttattgc caattaccca
      841 gacagaaaga cttttcttga atactcattc ggtagaagaa ttggaaacga aatttgatga
      901 agcgggtatt gattatttag atataaatcg tgcgagtact gtctggcagg caggataaaa
      961 attcggcaat caaaatgccg tctgaaacaa gtttggactt tcagacggta ttctgatgaa
     1021 tggaacagga tactgggaac tgagttcttc aaaaatccta tctccgtccc ttatacgtcg
     1081 gtgctttagt caaaacggca gacggatgaa aaattattgc ccgtatcctt gccaaagatg
     1141 aagcaagatg aagccaggta attgaatcgg tacaagcgca atgtccgact tgttctgcaa
     1201 aacagggagg gagattaagc caatattcaa aagaacagaa aaagaaactg agtaatggga
     1261 ataaaaaatg aacaaataat aatttgtaaa aaatataata cagaaatata tcctgtttcc
     1321 gatgtttcaa aaatcggtgt tgctgaaaat gtcaaacaga caggcctgta tccgattaac
     1381 gggttaaggc accgtcccaa aggtgatact aacggttggt atatctgggc cggtgaaaac
     1441 ttctcctatg ataaaaattt tttcctgccc ttgcatacgt tccatttgca aatatggcga
     1501 cccgaaatca tccccttttt gacacttccg cccggttacc gttttttaat tggggaaaac
     1561 ggttatgaag acgtatggtt tgatgaattg ctattaaatg ataattgacg aaaaatctac
     1621 ttcagatgta ctgaaacaat atcaagaact agggtttgat ttgacaaaac ctatgataat
     1681 agagtttttt ataggaggtt ctcaaaaaaa tctgcaatca atagaaagcc aactcatttc
     1741 ttatcggcaa gattttcaaa tcagtattga acaagatgaa ttcggagaaa tgtggacttg
     1801 ctattgttcc gtcaatatcg tcccttccct tgaaaacatt ttagctattg aaactacgtt
     1861 atttggtatt gcacaaaaaa atgattgtaa ttatgaaggt tttggttcgt atgggaacta
     1921 acaaaaagtg taggttgggt tgaaaaaacc atattcccat taaagattag gggatgtcta
     1981 tggaaataaa attcaatact ttgggggtta tcctgaatgg agtcaatcct gaagaaaaat
     2041 tcataaaaat tattgatgac caggaaaata ccggtggatt tctgatactg ttatcttcaa
     2101 atgacaaatt ctcatcattt gacagttacg atgactgggt cgaaaactta gaaatattaa
     2161 aagaatacct gcaagaatct cattggataa taaagtgggt cggatagtcg gcaaacgtag
     2221 tccgtttgaa acgcttttac ttttcagacg gactttctac caatgggaac aaggcgtttt
     2281 gtctcaatcc ggtttatgat tattcgatac agtgatggga agctaagaaa aatatgatta
     2341 atcatattga aaaatatata ggcactattt ctcatggctc gaaaagcgat tcgggaagtc
     2401 agtacaaatt aaatatcgca gctattccct cttcccctaa cagggattta aaaacttata
     2461 ttactttagg gttaagcaaa catgatctga attataaaag cagatttgaa atactttttg
     2521 tatgttcttt aaaatatgat gaaaatcaga ttttcccatt tttaagatgg ttagcagaaa
     2581 ccatcattga gaataaaaaa aatccttctt cgggggcagg ttgtttattt gcctagaagc
     2641 attgttaatt caacaaaaat ggacgcactg tatgtttccg ccccatttta tttcgacgat
     2701 gatttccaag tctgttatgg cgaacactac aatattgttt tccctttgct tgtccccttg
     2761 tataaacagg aagccgaatt ggtggaaaaa aagggttgga atgcttttga gcagttcttg
     2821 ttggataatg aagttggcaa cctttcggat atgaatagga agccgtttg
//
LOCUS       DAXDXW010000123         2775 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXDXW010000123 DAXDXW010000000
VERSION     DAXDXW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.124"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            444..662
                     /locus_tag="ACFB64_002049"
     CDS             444..662
                     /locus_tag="ACFB64_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210328.1"
                     /translation="MANSNSGHSKKLRAATAAAATKAKLASGEYRQFSVQGRAEDVEL
                     ILAAVEKAGGSRVQALAKICRRYLEGLS"
     gene            complement(679..1038)
                     /locus_tag="ACFB64_002050"
     CDS             complement(679..1038)
                     /locus_tag="ACFB64_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210329.1"
                     /translation="MQVLLGEDFKRALKNYPKEDRRKIAEFIAHVQQNGLSGLPGRNK
                     SSDNVPADDPQWLEKVRFAQRHNLWHYHIGIPKYNGGRYGDLTSAYILHYTLCDGFIK
                     IIGFDRHPPFILPDIPK"
     gene            complement(1039..1578)
                     /locus_tag="ACFB64_002051"
     CDS             complement(1039..1578)
                     /locus_tag="ACFB64_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HFC1210330.1"
                     /translation="MLNAYDVADFFLSPFEEEDGEQISNLKLQKLLYYAQGYALAILN
                     RPLFAENIEHWQHGPVVPCIYRTYKKYGGSPLPAAHIEPDKYADEELVVLNRVRKEQG
                     CYTAWALRNKTHQEAPWIQTRQGEVIGIALMGEYFRHALPQTDYNFNLEKLKTAVEDS
                     FVSVPHFNGADDLEKWLEQ"
     gene            complement(1738..2442)
                     /locus_tag="ACFB64_002052"
     CDS             complement(1738..2442)
                     /locus_tag="ACFB64_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1210331.1"
                     /translation="MTNRDLKAIRIDNLKRFFENKKLPVKDKSLLSQLMSGKASFGEK
                     VSRRLEEEYGMGSLYLDSVPSEPTPVLINPDLPHEVKDIHRPMMWSSNDPLPDDDYVF
                     VPYLKESCFKGGAGAYEIPDYNGYRLPFGKSTLRRKGINPDNVFCCTLTGDSMEEKIA
                     EDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSVFYPDETAPL
                     DSLSVIGRVFWRSVLD"
     gene            2570..>2775
                     /locus_tag="ACFB64_002053"
     CDS             2570..>2775
                     /locus_tag="ACFB64_002053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210332.1"
                     /translation="MTHSEFIKTLSEQRGAKTEYAKKLGLSLSFLWQIENGRAVTPKR
                     LYKDVMLLTKNKVSISELISEFRV"
BASE COUNT          674 a          604 c          711 g          786 t
ORIGIN      
        1 tttagtaaac acttaacatc tagtcaagta aaatgtttag tgattattta ataaatactg
       61 aacattttga ttattaaaag aatttatttt tgagattccg caggcgcaaa aaaaaccgcc
      121 tattaaggcg gttgtttagg aaagaatgcc ctctgcgcgt gtagggtatt tcgatacttg
      181 cgtttattat atcatatgat ataataaact catagattag gaaatgaaga aaccccgtat
      241 cagccgatac ggggcaacct gaaagagaaa ggaggcaatg tgattaagaa aatcctaatc
      301 tgtgtgattt tgtatttcct tgcaagctcc ccagcgtggt agggaaatac aaaacaagtg
      361 attaacgatt aaccatttgc agggcagtta aggaggctgc ccttcctctt atctttccca
      421 tactctacta taaggattta aaaatggcaa attccaacag cgggcattct aaaaaattgc
      481 gcgcggcaac ggcggcggcg gcgacaaagg caaaactggc aagcggcgaa taccggcagt
      541 tttcagtgca ggggcgtgcc gaagacgtgg agcttatcct tgccgccgtg gaaaaagccg
      601 ggggcagccg cgtacaggct ttggcaaaga tttgcaggcg gtatctcgag gggctgtctt
      661 aaggggggta agacaaggtt atttgggaat atcgggcagg ataaaaggcg ggtgtctgtc
      721 aaaaccgatg attttgataa agccgtcgca aagggtatag tgaaggatgt aggcggatgt
      781 caaatcgcca tacctgccgc cgttgtattt gggaatgccg atgtgatagt gccaaaggtt
      841 gtgccgttgg gcaaatcgga ctttttccag ccattgagga tcgtctgccg gtacgttgtc
      901 gctggatttg ttcctgccgg gtaaccctga taagccgttc tgttggacgt gtgcgataaa
      961 ttccgcaatt tttctgcggt cttctttggg gtagtttttc aacgctcttt tgaagtcttc
     1021 gccaagcagc acctgcattt actgctccaa ccatttttcc aagtcgtcgg cgccgttgaa
     1081 atgcgggacg ctgacaaagc tgtcttcaac ggctgttttg agtttttcaa gattgaaatt
     1141 gtaatccgtc tgcggcagcg catggcggaa atattccccc atcagcgcaa tccctatgac
     1201 ttcgccctgc cgggtctgta tccacggcgc ttcctgatgg gttttattgc gtaatgccca
     1261 agcggtgtag cagccctgct ctttacggac acggttgagg acaaccaact cttcgtccgc
     1321 atatttgtcc ggttcgatat gggccgcagg caatgggctg ccgccgtatt ttttgtaggt
     1381 gcggtaaatg caggggacta ccggaccgtg ctgccagtgt tcgatatttt cggcaaacag
     1441 ggggcggtta agtatggcaa gggcgtagcc ttgtgcgtaa tacaggagtt tttgaagttt
     1501 gagattggag atttgctccc cgtcctcttc ttcaaaaggg gaaaggaaga aatctgccac
     1561 gtcgtacgcg ttcagcattt tctgtttccg ttgttatttt tctgtatatt aaggccgccg
     1621 gcccattatt tcaagaaata atttgtgcaa attatccggt atcggcaggc tgtccgcgcc
     1681 gtcggcatat cgggcggata tatttgaaaa atacttgccc tgccgtttca gacggcatca
     1741 atccaacacg ctccgccaaa ataccctgcc gataacggac aggctgtcca aaggggcggt
     1801 ttcgtcggga tagaagacgc tgttgtggct gcgtatcagc acgctgttgc cgggctgccg
     1861 tatcaggtat ttgacgcgga acatgccgtc ttgggcgaag gcgtatattt tgccgtcccg
     1921 tatggcggtt tcgcccgtat ctacggcgat tgccgcgtct tctgcgattt tttcctccat
     1981 gctgtcgccg gtcagggtgc agcagaagac gttgtcgggg ttgatgcctt tgcgccttaa
     2041 cgttgatttg ccgaacggca ggcggtagcc gttgtagtcg gggatttcat acgcgcccgc
     2101 cccgcctttg aagcagctct ctttgaggta ggggacgaaa acataatcat cgtcgggcag
     2161 cgggtcgttg ctgctccaca tcatcgggcg gtggatgtct ttgacttcgt gcggcaggtc
     2221 ggggttgatg aggacgggcg ttggttcgct cggaacggaa tccaagtaca gactgcccat
     2281 cccatattct tcttcaagcc gtctgcttac cttttcgcca aaagatgctt taccgctcat
     2341 caattgagat aaaaggcttt tgtctttaac cggcaacttt ttattttcaa agaatctttt
     2401 taaattatcg atacggatgg ccttcagatc tctattagtc attttcgcgc tccattcaga
     2461 ttgggattca attttattta gtttctgcta aacaaacaaa tattaaattt tcttgcctat
     2521 gcgtttaata tttgctaaac tcattaaata tttacggtga aactacagaa tgactcattc
     2581 agaatttatt aaaacgctgt cagaacagcg tggtgcaaaa acggaatacg ctaaaaaact
     2641 tggcttatcc ctgtcttttc tatggcagat agaaaacgga cgggcagtaa cgccaaagcg
     2701 tctttataaa gacgttatgt tgttgacaaa aaataaggtt tctatttctg aattaatttc
     2761 ggagttcagg gtttg
//
LOCUS       DAXDXW010000124         2765 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXDXW010000124 DAXDXW010000000
VERSION     DAXDXW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.125"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(1..128)
                     /locus_tag="ACFB64_002054"
                     /pseudo
     CDS             complement(1..128)
                     /locus_tag="ACFB64_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
     gene            314..598
                     /locus_tag="ACFB64_002055"
     CDS             314..598
                     /locus_tag="ACFB64_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC1210333.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYAGVDRLAAHFQQAIT
                     HSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            599..1786
                     /locus_tag="ACFB64_002056"
     CDS             599..1786
                     /locus_tag="ACFB64_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFC1210334.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGVRRKVFTNI
                     KGLKIPHTHIETDAKKLPKSTDEQLSAHDMYEWIKKPENVGAIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVKRHYHIAANKMGLRTLLEW
                     KVCADDPVKMASSAFSSIYTLDKKVYDLYESAEIHTVNKVKRSKWFYALPVIILLIPL
                     FVGLSYKMLGSYGKKQEEPAAQESAATEQQAVLPDKTEGESVNNGNLTADMFVPTLPE
                     KPESKPIYNGVRQVRTFEYIAGCIEGGRTGCTCYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVQQSAQQHSDRAQVATLGGKPQQNLMYDNWEERGKPFEGIGGGVVG
                     SAN"
     gene            2001..>2765
                     /locus_tag="ACFB64_002057"
     CDS             2001..>2765
                     /locus_tag="ACFB64_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HFC1210335.1"
                     /translation="MNIIGSDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKVYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQQQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKS"
BASE COUNT          965 a          598 c          624 g          578 t
ORIGIN      
        1 ccaagggaaa aagaagcccc agctctgaaa tcatcacctt tattgaccgc acaacctccg
       61 ccgttccaat caaatgtgca acgatttaaa acaaaattat tccaatccaa ttaagcaact
      121 ttagccaaag gcaggcgaag catcgcactt tgcgggcgaa gccgcaaaca gccgagaagc
      181 gcgggggggg attggcgata agcgcgaggg ggtgtcccca cagcgctgcc gcgccgcgaa
      241 tgcggagcaa tctttcagat taagaaacat ttgtttaatg aggcaaacgt gccttttaag
      301 aaagggagag caaatgaaat tgttggccgc attgattccg ctcttgatga gcgtcgcagg
      361 ccgtatattg actgcattag gcttgatggc tgttacctat gcgggtgtag atagattggc
      421 agcccatttt caacaggcga taacccatag cataacgggc gcgcctcaag caatgttaca
      481 gcttttctat ataagcggcg gtggtactgt tctaaacatt cttttcggcg caatcgcctt
      541 tattctgtcc tttaaacaaa tgacaaaact cgcaacctca atcgggaaga aaaaataaat
      601 ggcagaaatc tgtttgataa ccggcacgcc cggttcaggg aaaacattaa aaatggtttc
      661 catgatggca aacgatgaaa tgtttaagcc agatgaaaac ggcgtacgcc gtaaagtatt
      721 tacgaacatc aaaggtttga agataccgca cacccacata gaaacagacg caaagaagct
      781 gccgaaatca accgatgaac agctttcggc gcatgatatg tatgaatgga tcaagaagcc
      841 tgaaaacgtc ggcgcaatcg ttattgtcga tgaggcgcaa gacgtatggc ccgcacgctc
      901 cgcaggttcg aaaatccccg aaaacgtcca atggctgaac acacacaggc atcagggcat
      961 agatatattt gtattgacac aaggtcctaa actcttagat cagaacttgc gaacattggt
     1021 taaaagacat taccacattg cggccaacaa aatgggtttg cgtaccctgc ttgaatggaa
     1081 agtatgcgcg gatgacccgg taaaaatggc atcaagtgca ttttccagta tctacacact
     1141 ggataaaaaa gtttatgact tgtacgaatc cgcagaaatt cacacggtaa acaaagtcaa
     1201 gcgttcaaaa tggttttatg cattgcccgt catcatatta ttgattccgc tatttgtcgg
     1261 tttgtcttac aaaatgttgg gcagttacgg aaaaaaacag gaagaacccg cagcacaaga
     1321 atcggcggca acagaacagc aggcagtact tccggataaa acagaaggag aatcggtgaa
     1381 taacggaaac cttacggcag atatgtttgt tccgacattg cccgaaaaac ccgaaagcaa
     1441 gccgatttat aacggtgtaa ggcaggtaag gacctttgaa tatatagcag gctgtataga
     1501 aggcggaaga accggatgca cctgctattc gcatcaaggg acggcattga aagaagtgac
     1561 ggagttgatg tgcaaggact atgtaaaaaa cggcttgccg tttaacccat acaaagaaga
     1621 aagccaaggg caggaagttc agcaaagcgc gcagcaacat tcggacaggg cgcaagttgc
     1681 caccttgggc ggaaaaccgc agcagaacct aatgtacgac aattgggaag aacgcgggaa
     1741 accgtttgaa ggaatcggcg ggggcgtggt cggatcggca aactgaagaa aacggcaaca
     1801 gagaaaaaag acccgtaaac cgtttgaata tagacggctt acgggtcttt gtttcgcgca
     1861 aagcaagggc taaggcagtc aggcagcaaa tcccgcaatg tattaaaaca gacgcgtaga
     1921 aatgccggct gcctttatcc atcctcaaaa ttgaatatca tcctagccgt atcaaggctg
     1981 tataaataag gaaaatacca atgaatataa tcgggtcgga catctcaaag gacaccatag
     2041 acgcaacatt gcataaaaca aacggaagta tccattacat taaatttaag aataatgatg
     2101 atggattaaa acagtttaga ttgtggataa agggaaacag aatcagaaaa gtctatatcg
     2161 gcatggaggc aacaggcatc tattacgaaa aggcagcaga tatgctttct tcctactata
     2221 ccgtttacgt tatcaatccc ttaaaaatca aggactacgg aaaaagcagg tttaaccgca
     2281 ccaaaaccga caaagcagat tcaaacctga tagcagatta cataaaaagg catcaagata
     2341 cattgatacc gtatcagata cccaaaaaca aagcactgca aaaactgatt aatcttaaaa
     2401 accaattaca gcaacagcag aagcaaatta aaaaccgtct tcatagcact gaagaagact
     2461 tcataaggaa catacatcaa gacttgatag ataccataca ggacaagatg gaacaggtaa
     2521 aaatagccat atccgaacaa atcaaaaaac aaacggacaa taaccattac cgcaatcttc
     2581 aaaccatccc gagcataggc aaagacaccg catcagttct ttatgcgcaa ctgacagaaa
     2641 aacattttaa aaccgcaaac caatttgtat cctatgccgg attaagtccc gccatcatac
     2701 aatcagggac aagcgtaaga ggtcggggca gattgagccg atacggaaac agacgattaa
     2761 aaagt
//
LOCUS       DAXDXW010000125         2690 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXDXW010000125 DAXDXW010000000
VERSION     DAXDXW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.126"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(46..1029)
                     /gene="porB"
                     /locus_tag="ACFB64_002058"
     CDS             complement(46..1029)
                     /gene="porB"
                     /locus_tag="ACFB64_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_218015349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB.IA"
                     /protein_id="HFC1210336.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVAYHGAQA
                     DRVKTTTEIADLGSKIGFKGQEDLGNGLKAIWQLEQKAYVSGTKEGWGSRQSFIGLKG
                     GFGKVRVGHLNNILKDTDGFNPWEGKSYYLSLSNIAQPEERHVSVRYDSPEFAGFSGS
                     VQYVPNDNSGKNRSESYHAGFNYKNSGFFVQYAGSYKRHNYTTEKHQVHRLVGGYDHD
                     ALYASVAVQQQDAKLTWSNDNSHNSQTEVAATAAYRFGNVTPRVSYAHGFKGSVYDAD
                     NDNTYDQVVVGAEYDFSKRTSALVSAGWLQRGKGTEKFVATVGGVGLRHKF"
     gene            complement(1546..2343)
                     /gene="truA"
                     /locus_tag="ACFB64_002059"
     CDS             complement(1546..2343)
                     /gene="truA"
                     /locus_tag="ACFB64_002059"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC1210337.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQAGGVPTVQAALETALARI
                     AGESVATTVAGRTDTGVHAAAQVVHFDITAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PGFHARFDASGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAALLVGEQDFS
                     SFRAAGCQAKSPVKTIYRAGLTQSAGLIRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPGAYGIVRPQIPEWL"
BASE COUNT          543 a          642 c          812 g          693 t
ORIGIN      
        1 agcctgtctg gcgacaggct ttttgttgat accaatcttt gcagattaga atttgtggcg
       61 cagaccgaca ccgccgacag tcgctacgaa tttttctgtg cctttgcctc tttgcaacca
      121 accggcagaa accaaggcag aagtgcgttt ggagaagtcg tattccgcac cgacaaccac
      181 ttggtcgtaa gtattgtcgt tatctgcatc ataaaccgaa cctttgaagc cgtgggcgta
      241 agaaacgcgg ggcgttacgt tgccgaagcg gtatgccgcg gtagcggcaa cttcggtttg
      301 agagttgtgc gaattatcgt tgctccaagt caatttcgcg tcttgttgct gtacggctac
      361 ggaggcgtac agggcatcat ggtcgtaacc gccgaccaaa cggtgaacct ggtgtttctc
      421 agtcgtgtaa ttatgtcttt tataggagcc ggcatattgc acgaagaagc cgctgttttt
      481 gtagttgaag cctgcatggt aagattcgct gcgatttttg cccgaattgt cgttaggcac
      541 gtattgtacg ctgccgctga agccggcaaa ttcgggagaa tcgtagcgta cggaaacgtg
      601 gcgttcttcg ggttgggcaa tgttgcttaa actcaaatag tagcttttac cctcccaagg
      661 attgaagccg tcggtgtctt tcaggatgtt gttcaaatgg ccgacgcgca ctttaccgaa
      721 gccgcctttc aaaccgatga aggattggcg gctgccccag ccttctttag taccgctgac
      781 gtaggctttt tgttccaact gccaaatggc tttcaggccg ttgccgaggt cttcttgacc
      841 tttaaagccg attttcgaac ccaaatcagc gatttcggta gtggttttaa cgcgatccgc
      901 ctgagctcca tggtaagcta cggagcggga agtttctacg ccggctttga tggtgccgta
      961 cagggtaacg tcagccattg ctgcaacagg aagggctgcc aaagtcaggg caatcaggga
     1021 ttttttcatt gctgtattcc ttttttggtt aagaaattta agcaggccgg gctttccaag
     1081 ccgcttagct ttgcatttac cgccgagggg ctcggcggtt acatgtctga aactatagat
     1141 gttcctgtct gaaaaaacaa acttctgcct gtttttgtgt ggaatccgat gtgcattttg
     1201 aaggacggat agagatatta cgggtatttt ttgttttgta acatatggtt atttaaaatt
     1261 tttaagattt gcatttttac aacacttact cgggagggta ttggagggca ttgcaaaccg
     1321 ggggttatag cggattaaca aaaaccggta cggcgttgcc ccgccccggc ccaaagggaa
     1381 cggttcccta aggcgcccaa gcaccgggcg aaccggttcc gtaccatttg tactgtctgc
     1441 ggcccgccgc cttgtcctga tttttgttaa tccgctatag tgtcaacatt gaaaaagatt
     1501 gcctgacagt ttgtccgatt tcaaaatctc cgcgacaaga atgttttaaa gccattcggg
     1561 gatttggggg cggacgatgc cgtatgcccc gggatagtcg acgccggtca ggtaaagccc
     1621 gtcgggcatg aaggtcggtg gggctttgag gcggctgcgt tcttgaatca gtgcggcgaa
     1681 gccttcgacg ctgagcctgc cgctgccgac ataaacgagc gcgcccatga tgttgcgtac
     1741 catgtggtgc aaaaaggcgt tgccgtgcaa atcgaggcgg atcagtcctg cgctttgggt
     1801 aagcccggcg cggtagatgg ttttgacggg ggattttgcc tggcacccgg cggcgcggaa
     1861 gctggagaag tcttgttcgc cgaccaataa ggcggcagcc cggcgcatcg gttcgatgtc
     1921 gagtttgagg tgtgtccagc ccgccctgtt tttcaacagc ggcgaacgga cgggggcgga
     1981 ttcgagcagg tagcggtagt gccgtccgga tgcgtcgaag cgggcgtgaa atccgggtgc
     2041 gacctgtcgg gcgtgcaaaa cggcaatgcc ttcgggcagg tgggcattta cgccgcgcac
     2101 ccatgcctgt tggggacggg cggcagttat gtcaaagtgg acgacttggg cggcagcgtg
     2161 cacgccggtg tcggtcctgc cggcaactgt ggtggcgaca gattctcctg cgattcgggc
     2221 gagtgcggtt tccaatgccg cctgaacggt cggtacgccg cccgcctgtt tctgccagcc
     2281 gtaaaagcgg ctgccgtcgt aggatagggt tattgcccag cgttgttttt gtgcggtatc
     2341 catcgggttt gggattcgga taaatgttca gactgcattg tatcgcagat tttgcaggga
     2401 aacggcaaac gcccggggcg cgcggcgttg tttggggagt tgttcggggg gttggatgca
     2461 gttgctacga atcgctatcc tgtgaattca ccctgtcagg agtgcccgaa ccgtcattcc
     2521 cgcgcaggcg ggaatctagg tctgtcagtg cggaaactta tcgggtaaaa cggtttcttg
     2581 agatttttcg ttccggattc ccacttttgt gggaatgacg ggatgcaggt tcccgtgccg
     2641 acgggtctgg attcccgctt atgcgggaat gacggcggcg ggaatcggtg
//
LOCUS       DAXDXW010000126         2683 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXDXW010000126 DAXDXW010000000
VERSION     DAXDXW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.127"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(234..647)
                     /locus_tag="ACFB64_002060"
     CDS             complement(234..647)
                     /locus_tag="ACFB64_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210338.1"
                     /translation="MIHLNVHYCDEIIRHAENDKYSLIGIFPDICHIPTPQAILGRLC
                     LSVSFSAEGMDIQTMKTGQIFLEIVRNDDVISALEIPSYDGSDTEENVSFMLHQTISG
                     LPVSDNDRIYVRMTTHNHILSESRPLSFSWLPYHS"
     gene            complement(644..1105)
                     /locus_tag="ACFB64_002061"
     CDS             complement(644..1105)
                     /locus_tag="ACFB64_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1210339.1"
                     /translation="MGATLTVAVAACTVSTPTSAIPVTHIKCLRINGQIKCVKPISPN
                     TTPAAEHIEHVRKNPRRKAAMDRAAARIADKIALKAGGETFVSLRMKKGFTQSELATA
                     AGLPQPYLSRIENSKQSLQDKTVQKLANALGVSPLEVRAAFERRYEYMEQA"
     gene            complement(1122..1559)
                     /locus_tag="ACFB64_002062"
     CDS             complement(1122..1559)
                     /locus_tag="ACFB64_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210340.1"
                     /translation="MELTVHFNAEQDLDRLFEKDEEAVGYLENVIAMIQADSAIFDGL
                     YKNRYFREYGEPIGPIDLEVKPILSLWGKDIKVLRVRFDSEEAAGYRIIYAPCHEKQP
                     NGTYIRRIDILAVVNKKTDEFDYQAEHPITKRIIKDYEELYSN"
     gene            complement(1674..2390)
                     /locus_tag="ACFB64_002063"
     CDS             complement(1674..2390)
                     /locus_tag="ACFB64_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFC1210341.1"
                     /translation="MPFIIDYQCLCAINSEIKMFSGEQLGQAISEAIKRKNVSQKEVA
                     DHFGVKQPSVSGWIKNGRIDKKHLDKLIDYFSDVVTPSHFGIETFRVLKSNEQSSIRF
                     PRLNAEATCGAGTINDHYIEVVDYVTVAAAWAREKLGGNLNKIQVITARGDSMEPTIE
                     NGDVMFVDTAVEAFDGDGLYLLWYIDGLKAKRLQSTVGGGLMIISDNSSYRTETVRGE
                     DLNAVRIIGRIRGAWRLSQF"
     gene            2459..>2683
                     /locus_tag="ACFB64_002064"
     CDS             2459..>2683
                     /locus_tag="ACFB64_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC1210342.1"
                     /translation="MAMSIQKAVDYFGNESRLARAIGVKQPTVWAWNKKGTPPPIIRC
                     VQIEKLTGGAVNRKDLRPDDWHLIWPELAGD"
BASE COUNT          647 a          576 c          640 g          820 t
ORIGIN      
        1 gccaaaccga tttattctgc tgattaattc gttcatcccg tttccttcaa gttgttgttt
       61 gtttcgatgg gtgtattata actattacct atatttaatc aataggcaat agttatattt
      121 tcatccatat atacttattg aattgatttt tagaagaaaa aagtttgaaa aaaaaccgcc
      181 cgaatggcgg tgtatcatga aaaagaaaac cgccctaggg cggttcggta ttgttatgag
      241 tggtaaggaa gccaagaaaa agacaaagga cggctctcgg agagaatatg gttgtgtgtt
      301 gtcattctta cataaatgcg gtcgttgtct gaaacgggta gtccgctgat tgtttggtgt
      361 agcatgaatg acacgttttc ttctgtatca ctgccgtcat atgatggaat ctcaagtgca
      421 gagattacat catcgttgcg gacgatttcc agaaaaattt gaccggtttt catagtctgt
      481 atatccatgc cttcggcgga aaatgaaaca gacaggcaca ggcggcctag aatggcctgc
      541 ggtgtcggaa tatggcatat atcggggaat atacctatca ggctgtattt gtcattttcc
      601 gcatggcgga taatttcatc gcaatagtgg acatttaagt gaatcatgcc tgctccatat
      661 attcgtaccg ccgttcgaac gctgcgcgaa cttcaagcgg ggaaacgcct aatgcgtttg
      721 ccaatttttg tacagtctta tcttgcaggg attgtttgct gttttcgatg cgggacagat
      781 agggttgagg caatcctgcg gcggttgcca attcggactg ggtaaacccc tttttcatcc
      841 ttaagctgac aaatgtttcc ccgcctgctt tgagggctat tttatcggca attcttgctg
      901 ccgccctgtc catcgctgct ttgcggcgcg gatttttccg cacatgctca atatgctctg
      961 cggcaggggt agtgttaggg cttatcggtt tgacgcattt aatctgcccg tttatccgca
     1021 ggcattttat gtgggtgacg gggatggcgg atgtaggtgt tgatactgtg caggcggcca
     1081 cggcaacagt gagtgttgca cccattttga accatgtgcc gttagttgga gtacagttct
     1141 tcatagtctt tgataatccg ttttgtgatt gggtgttctg cctgatagtc aaattcgtct
     1201 gtcttcttgt ttacaacagc caatatatct atgcggcgga tatacgtgcc gtttggctgt
     1261 ttttcatggc atggtgcgta aattattctg tatccggcgg cttcttcgct gtcaaatcgg
     1321 acgcgtaaaa ctttgatgtc ttttccccat aatgacaata tgggtttcac ttccaagtca
     1381 atcgggccta tgggttcgcc atattccctg aagtatctgt ttttgtataa gccgtcaaaa
     1441 atagcagagt ccgcctgaat catcgcaatg acattctcga gataaccgac cgcttcttcg
     1501 tctttttcaa agaggcggtc taaatcctgc tcggcgttaa agtggacggt cagctccatg
     1561 agtgcccttt ttatatatat atattatatc tttttagtta taccgcgcaa tatgcggtaa
     1621 taaatcagtt gttcaagtat tttaatccgg cacgctccac cagaagacgc ggtctagaac
     1681 tggctcaaac gccatgcgcc gcgtatgcgt ccgatgatgc gtacggcgtt taaatcttcg
     1741 ccgcgcacgg tttcggttcg gtatgagctg ttgtcgctga tgatcatcag gccgccgccg
     1801 acggtggatt gcagccgctt ggccttaagg ccgtctatat accaaagcag gtagaggccg
     1861 tcgccatcga aggcttcgac ggcggtatca acgaacatta cgtcgccgtt ttcgatggtg
     1921 ggctccatgc tgtcgccacg ggctgtaatg acttggattt tgttgaggtt tccgcccagt
     1981 ttctcccgcg cccatgcggc agcgacggtt acataatcca caacctcgat atagtggtcg
     2041 ttaatcgtgc ctgcgccgca ggtcgcttcg gcatttaagc gggggaaacg tatgctactt
     2101 tgttcattcg attttaagac tctgaatgtt tcaatgccga aatggcttgg cgttactacg
     2161 tctgagaaat aatcaattaa tttatctaga tgttttttat ctattcgtcc attttttatc
     2221 caacctgaaa cgcttggctg tttcacccca aaatgatcgg caacctcctt ttgactgaca
     2281 ttttttctct taatcgcttc tgatattgct tgtcctaact gttcgcctga aaacattttg
     2341 atctccgaat taattgcgca taagcattgg taatcgataa taaaaggcat aacctataaa
     2401 aggcaatagt tgtatttaat ataagtatta gctataatga agctatttaa ttgagtaact
     2461 ggctatgagt atccaaaaag cagttgatta ttttggtaat gaatcccgac ttgcacgggc
     2521 gatcggagtt aaacaaccga cggtgtgggc ttggaataaa aaaggaacgc cgcccccgat
     2581 cattcggtgc gtgcagattg aaaaattaac cggaggcgca gtgaatcgaa aagacttacg
     2641 tcctgatgac tggcatctaa tctggccgga attggcaggc gac
//
LOCUS       DAXDXW010000127         2645 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXDXW010000127 DAXDXW010000000
VERSION     DAXDXW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.128"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(46..705)
                     /locus_tag="ACFB64_002065"
     CDS             complement(46..705)
                     /locus_tag="ACFB64_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFC1210343.1"
                     /translation="MTPPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRALLEMPSEAAVADIARTYSAHYLNPNNRNMTLFPDALPCLDKLKAQ
                     GFRLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGLCGELGLDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLGAPHLAVLDGLSELPGFLARHY
                     A"
     gene            865..1080
                     /locus_tag="ACFB64_002066"
     CDS             865..1080
                     /locus_tag="ACFB64_002066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210344.1"
                     /translation="MSVFRINMAAATVLAALSSPVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSLKAAIWMTASIPASPPAA"
     gene            1026..1569
                     /locus_tag="ACFB64_002067"
                     /pseudo
     CDS             1026..1569
                     /locus_tag="ACFB64_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            1639..2569
                     /locus_tag="ACFB64_002068"
                     /pseudo
     CDS             1639..2569
                     /locus_tag="ACFB64_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          615 a          767 c          742 g          521 t
ORIGIN      
        1 aggcttcaga cggcattttt gccgcgtgcc ggatgcggaa accaatcagg cgtaatgtcg
       61 tgcaagaaaa ccgggcagtt cggacaaacc gtccaatacg gcgagatgcg gtgcgccaag
      121 gagctgttcg cgcgaatgtg cgccggtggc aacgccgact gccgccgcgc ctgcgtttgc
      181 cgccatatgc aggtcgtgcg ccgtatcgcc gacaaccaat gcctctttcg ggtcgagtcc
      241 cagttcgccg cagagtccga ataccatttc gggcgagggt ttggaggggt attcccccgc
      301 gcaggcggtg gccagccaat agccgccggt ggcggtttgg ctgatggcgt tgtccaaacc
      361 cgcccgccct ttgcccgtgg cgacggcaag ccggaagcct tgcgctttga gcttgtccag
      421 acagggcagg gcatcgggaa agagtgtcat attgcggttg ttgggattga ggtaatgcgc
      481 ggaataagtg cgcgcgatgt cggcaacggc agcttcagaa ggcatttcga gcagggcgcg
      541 gatgatttcg ggcaggctgt atccgatcag gctgcggacg cgctccgctt cgggcggcgg
      601 aaaaccgcat tcggcgaagc tgcggcgcat ggtgtcgatg atgggctggg tcgtatcggc
      661 aagcgtgccg tcccagtcga agatgatgag tttgggcggg gtcatggcag gttggttgca
      721 gtaaaaaagc agattttatg cggaaaacgc agacgtgtcg cattttcgac aaaatttgtc
      781 ggctgagcga tatgtttttc cgaacaagcc gcgttgtgct ttattaaaat agaaccatta
      841 tcatttatac agatgggaca gtttatgtca gttttccgca tcaatatggc cgccgccacg
      901 gttttggcag cactctcgtc tccggttttt gccgcacaaa cggcggattt ggaaaccgtc
      961 catatcaaag ggcagcgttc gtacaacgcg attgccaccg agaaaaacgg cgattacagc
     1021 tcgttaaagg cagctatatg gatgaccgcc tcaatacccg cgtctccccc tgccgcctga
     1081 aagacaaaaa cgccgccgaa cccgaacaac cgcaacaccc gttacgccgc attgggcaaa
     1141 cgcgtgatgg aaggcgttga gaccgaaatc agcggtgcga ttacaccgaa atggcaaatc
     1201 catgcaggtt acagctatct gcacagccaa atcaaaaccg ccgccaatcc acgcgacgac
     1261 ggcatcttcc tgctggtgcc caaacacagc gcaaacctgt ggacgactta ccaagttacg
     1321 cccgggctga ccgtcggcgg cggcgtgaac gcgatgagcg gcattacttc atctgcaggg
     1381 atgcatgcag gcggttatgc cacgttcgat gcgatggcgg cataccgctt cacgcccaag
     1441 ctgaagctgc aaatcaatgc cgacaacatc ttcaaccgcc attactacgc ccgcgtcggc
     1501 ggcacgaaca cctttaacat tcccggttcg gagcgcagcc tgacggcaaa cctgcgttac
     1561 agtttttaaa gaccaatatg ccgtctgaaa cggcagccgc agcataatca aaccacaaca
     1621 agctgcgcgg cataccctat gcgctcacaa ccggagtatg gcattgcgaa ggaaaacaga
     1681 ccgaaccggc aggcagaccg ctttgccggt tcggttttac cgcttgccgc cagtctgacc
     1741 cacaagccga acatcatgaa acccataccg accgacacat tccaacctgc catactgccc
     1801 caagcctttg aaaccgaaat caaatccacc tgcacggggc gaatctatcg gattcagacg
     1861 gcaacactcg gcgaaatgca gtctgaaggc tatcccgtcc tctttgtcct cgacggcgaa
     1921 gcctttttcc cgcgctgtac aacatcatgc agtcgctgat gaacaacccc gttacccgaa
     1981 gcaacgcccc ctgcctgatt gtcggtatcg gctacacgac aggcagtgtg cgcgacctgg
     2041 cgcagcgtgc cgccgactac acgccgccgc ttggagacaa cgccacagca gacgaacggc
     2101 ggcagttcgg acaggcagac cgcttcgccg actttatcga cagcgaactg accgcctttt
     2161 tagaaagccg ctacaccctt aaccgtaatg aaaccgccgt attcggacac tcgttcggcg
     2221 cactgttcgg actgtattcc ctgctttccc accgctgttt cagacggcat tggctcgtat
     2281 cccctcgatt tggtggcaca acaggcggat actcgacttt atgccgtctg aaaaccggct
     2341 ggacggcatc gatgcctgcc tcaacatcgg cgcactcgag cggagcagcg gttgcaaacg
     2401 cagggaagaa cgcgacatgg cagggcaggc cgaacaaatg gcggcagagt tggacaggcg
     2461 cgggaccgcc gtatttttcc gggaatatcc gaatgccgac cacggcaatg tcccgtttta
     2521 ctcgctgacc gactgcgtcg aatatttgag ggaagcgtgg caacggtagg gggaatcaaa
     2581 tatatgactg ctttgttttg catcggaaaa tatagcggat taacaaaaac cggtacggcg
     2641 ttgcc
//
LOCUS       DAXDXW010000128         2627 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXDXW010000128 DAXDXW010000000
VERSION     DAXDXW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.129"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            68..205
                     /locus_tag="ACFB64_002069"
     CDS             68..205
                     /locus_tag="ACFB64_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210345.1"
                     /translation="MSREQRIRGARLPVSAYMQAEGAGKAREALDGRVEKVKGVDDEA
                     V"
     gene            189..1253
                     /locus_tag="ACFB64_002070"
     CDS             189..1253
                     /locus_tag="ACFB64_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210346.1"
                     /translation="MMKPSESLRAAGRPIAYYPKLAKPLGGVNAAILFGHFFYWNDKT
                     QYESGIYRTAEEIEIETGLSVQEQRTARAKLRERGVLIETEKRIEHRIYYKLNLDAFD
                     DLMLQHSGGGESTAPKCNINSPELQNQHSGGGESTAPKCNINSPELQNQHSGSEESTA
                     VIRTEDLTEDLAVYTPLPPNAGNGKGGLNADAFVSADAETCGRETGEPTSPKAESDSN
                     GNGGLSGKPKNANVPRRRKTHGVPLQEIADLYNEVLGGRLPSVQVLNDTRKRAIANRW
                     CEMLGTAAPNGKVRFGDKETGLAWFAGFFRKVAMNPFWMGENQTGFAVGFDWIFKAGN
                     FVKILEWHPPKTNQAARGRA"
     gene            1250..2611
                     /locus_tag="ACFB64_002071"
     CDS             1250..2611
                     /locus_tag="ACFB64_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFC1210347.1"
                     /translation="MNRIEETEAVQSLASVGAEQNILGGILIEPTAIARCAILTPEKF
                     YQAQHRIIFRALLDMAAANEPIDIITLNDKLEARGEAENAGGLAYLIDLNQNTPSAKN
                     ISRYVGIVNDRFVERGLLKASAAIEKIAVSKDGGTVAEKLSKAADELAAAGKDAVKRE
                     TKTFGQTVEDLIGGLDKRLDGVRFGLPTGLMKLDGMTGGLPDGNLIVIAARPSMGKTV
                     LAENIARFALKQGKAVHFQSYEMSAVELARRGMAAECNIPMQNLKTGNLTQSDYANMP
                     IYVSQAKEWKFDVNCDLLNVDELCFLAKEKKLTTGLDLLVVDHLHIMPRAGRDEVAEL
                     GNISRRLKNLAAELNIPVVLVAQLNRGNTKQADKRPNMADIRGSGAIEQDANIIIMPH
                     RESYYDGNENPSIAELIIAKNRDGETGTVVCGWKGQFMKFEEEPDLAWQAPKHDEYDP
                     YSV"
BASE COUNT          722 a          613 c          799 g          493 t
ORIGIN      
        1 aatacaaggt cggtaactgt aaaaatcccg ttgcccatta ctcaatcgca aagtcaggaa
       61 ttgaaccatg agccgggaac aaagaatacg cggagcccgc ttgccggtgt cggcttatat
      121 gcaggcggaa ggcgcgggca aggcacggga agcgttggac gggcgggtcg aaaaagtgaa
      181 aggggttgat gatgaagccg tctgaaagtt tgagagcggc aggcaggcca atcgcgtatt
      241 acccgaaatt ggcaaagcct ttgggcggtg taaatgcggc aatattattc gggcatttct
      301 tctactggaa cgataagacg cagtatgaat caggcattta ccgaacagcg gaagaaattg
      361 aaattgaaac cgggctgtcc gttcaggaac aaagaacggc acgggcaaag ctgagggaac
      421 gcggcgtatt gattgagact gaaaaacgaa ttgaacaccg catttactac aaactgaatt
      481 tagacgcttt tgatgatttg atgttgcaac attcgggggg tggggaatca acagccccga
      541 aatgcaatat caacagcccc gaacttcaaa atcaacattc ggggggtggg gaatcaacag
      601 ccccgaaatg caatatcaac agccccgaac ttcaaaatca acattcgggg agtgaggaat
      661 caacagccgt tataagaaca gaagatttaa cagaagattt agcagtatat acccccttac
      721 ccccaaacgc cggaaacggc aaaggcggtt tgaacgctga cgcgtttgtt tccgctgacg
      781 cggaaacgtg cgggcgggaa accggcgaac cgacttcgcc gaaggccgaa agcgacagta
      841 acggcaacgg cggcctttcg ggaaaaccga aaaatgcgaa tgttccgcgc cgccgcaaaa
      901 cccacggcgt accgcttcag gaaatcgccg atttgtacaa cgaagttttg ggcggccggt
      961 tgccaagcgt ccaagtgctg aacgacacgc gcaaacgggc gattgcaaac cgctggtgcg
     1021 agatgctggg aacggcggcg ccaaacggca aggtgaggtt cggggacaag gaaaccggtt
     1081 tggcctggtt tgccggtttc ttccggaaag tggcgatgaa cccgttttgg atgggcgaaa
     1141 accaaacagg gtttgcggtc ggcttcgatt ggattttcaa ggcgggcaat ttcgtcaaaa
     1201 tccttgaatg gcatccgcct aaaacgaacc aggcggcaag gggaagggca tgaaccgaat
     1261 cgaggaaacg gaagcggtcc aatcactggc cagcgtaggg gcggaacaga acattttggg
     1321 cggcatcttg attgaaccga cggcgattgc gcggtgcgca atcctgaccc ctgaaaagtt
     1381 ttaccaggcg caacacagga ttattttccg cgctctgctg gatatggcgg cggcaaacga
     1441 gcctatcgac atcatcacgc tgaacgacaa gctggaagcg cgcggcgagg cggaaaacgc
     1501 gggtggcctg gcttacttga tagacctgaa ccaaaacacc ccaagcgcga agaatatcag
     1561 ccggtacgtt gggattgtga acgacaggtt tgtcgagcgc ggcttgctga aggcttcggc
     1621 ggcgattgaa aaaatcgcgg tttccaaaga cggcgggacg gtcgcagaaa agctgtctaa
     1681 ggcggccgac gaattggcgg cagccggcaa agacgcggta aagcgtgaaa ccaagacatt
     1741 cggccagacc gttgaggatt tgattggcgg tttggacaaa aggcttgacg gcgtgcgttt
     1801 cggattgcct accggcttga tgaagcttga cgggatgacc ggcggtttgc cggatggaaa
     1861 cctgattgtg attgcggcgc gtccgtctat gggtaaaacg gttttggcgg aaaacattgc
     1921 gcgattcgcg ctgaagcagg gcaaggcagt tcatttccaa agctacgaaa tgagcgcggt
     1981 agagctggca aggcgcggca tggcggctga gtgcaatatc cccatgcaga acctgaaaac
     2041 cggcaatctg acgcaaagcg attacgccaa tatgccgatt tacgtcagcc aagcgaaaga
     2101 gtggaagttt gacgtgaact gcgacctgct caacgttgac gagctttgct ttttggctaa
     2161 ggagaaaaaa ctcactaccg gcttggattt gttggttgtc gatcaccttc acatcatgcc
     2221 aagggcaggg agggacgagg tggcggagtt ggggaatata tcgcgccgtt tgaaaaactt
     2281 ggcggcagag ctgaatatcc ccgtcgtctt ggttgcccag ttgaacaggg gaaacacaaa
     2341 gcaggcagac aaacgcccga acatggcaga cattcgcggc agcggcgcga ttgagcaaga
     2401 cgcaaacatc atcatcatgc cgcaccgcga aagctactac gacggaaacg agaatccgag
     2461 cattgccgag ctgattatcg ccaagaaccg ggacggcgaa acgggaacgg tggtttgcgg
     2521 ctggaaaggg caatttatga agttcgagga agagcctgat ttggcatggc aagcccccaa
     2581 acatgatgaa tatgaccctt acagtgtcta gtgcgggagg ccggtaa
//
LOCUS       DAXDXW010000129         2595 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXDXW010000129 DAXDXW010000000
VERSION     DAXDXW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.130"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(167..556)
                     /locus_tag="ACFB64_002072"
     CDS             complement(167..556)
                     /locus_tag="ACFB64_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC1210348.1"
                     /translation="MNPPEKLLTAENPALRQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTVYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILRVLQELEKQPAR"
     gene            complement(719..1098)
                     /locus_tag="ACFB64_002073"
                     /pseudo
     CDS             complement(719..1098)
                     /locus_tag="ACFB64_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1152..2285)
                     /gene="carA"
                     /locus_tag="ACFB64_002074"
     CDS             complement(1152..2285)
                     /gene="carA"
                     /locus_tag="ACFB64_002074"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC1210349.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSASGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSSFRASESLHDYL
                     VRNETVAIADIDTRRLTMLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDKQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKQA"
BASE COUNT          527 a          640 c          750 g          678 t
ORIGIN      
        1 ctctcccaca gggagagagg acgggtcggc tgttggggtt gaggtttgct gcaaggaaaa
       61 caggttgtgc aggttgcttt tttgcttttc agacgacctt tttaaacgct taattcagtt
      121 aattttacct tatttttttc aaaatctaat caaaatcaac cgtttgctac cgtgctggct
      181 gcttttccaa ttcctgcaat acgcgcagga tttccgccag tacatcgttt gtctgctgca
      241 aaatttcgtg attccaaaaa cgcagcacgg taaagcccaa gctgttgaga tataccgtcc
      301 gcgcgtggtc gtatacggct tgttccgcgt gctgcccgcc gtctgcttcg acaatcagct
      361 tgggcgttac gcacataaaa tcaacaatat aattccccat cggctgctgt cggcggaatt
      421 tatagccgtt cagacggcct gcccgcaggt gttgccacaa ttttgcttcc gcctcgctca
      481 tttcttgacg catggctttg gcgcgttggc gcagggcggg gttttcggcg gtcaatagtt
      541 tctcgggcgg gttcattttt gttatcgggt tgctgtttgt gtaagcctta cagcttgttg
      601 ccctctctct aactctttcc cacagggaga ggggacgggt cggctgttgg ggttaaaggg
      661 gctgcaagga aaacagcttg tgcaggctgt tttttatttt gcataatccg tctgaaaatt
      721 aataactgct gatttccagc aggttgccat cgggatcgcg caggtaaacc gattggattt
      781 tgcccattgc gcctgtgcgc gctacgatgc cgcttaaagg tttgatgccg tgtgcggata
      841 attcctgtaa aaccgtttcc agtggggcgt atcggtcagc aggcataaat ccgctgtgcc
      901 gcaggcggcg tgttgcgcgt taggctgaat ttccgcaccg cgcccgtgta ggttgatttt
      961 ctgactgtca aacaacaaag ctttacggtt gttgccaaat gaaacttctt ccatgcccaa
     1021 aacttgtgtg taaaacgcga tggttcggtc aatgtcggca acagtcagta ctagatggtc
     1081 gagtgcgcta attttcattt ccagctttct ttttcagacg gcagcagcat actgttgccg
     1141 tctgaaaatc attatgcttg ttttgcagcc ttcatattgc caatgaattt gtcgaacaaa
     1201 tagccgacat cttgcggacc cgggctggct tcggggtgtc cttggaaaca gaacacgggt
     1261 ttgtcggtca gctcgatgcc ttgcaaggta ttgtcgaaca aggatttgtg ggtaatgcgt
     1321 gcgttggcgg gcagggtgtc ggcatcgacg gcgaaaccgt ggttttggct ggtaatgacg
     1381 actttgccgc tgtccaaatc ttgcacagga tggttcgcac cgtggtggct gaagcgcatt
     1441 ttcagggttt tcgcgccgat ggcgaggctg atgagctggt gtcccaagca gatgccgaaa
     1501 atcggtttgc cgctttccat cagtttttgc acggcttcga tggcgtaggt gcaaggctcg
     1561 gggtcgccgg ggccgttgga caggaacacg ccgtcgggat tgagtgccaa cacgtcttcc
     1621 gcgctcgttt gtgcggggac gacggtcagg cggcagccgc gcgaggcgag catacgcagg
     1681 atgttggttt tcacgccgaa atcgtaggcg acgacgtggt aaggctgttt gtcaggggta
     1741 acgaaacctt tgcccaattc ccattcgcct tccgtccatt cgtaagtttc cgtgcaggaa
     1801 acttcttttg ccaaatcttt gccgaccatg ctgccgaatg cggcgatgag ttcttgcgct
     1861 ttttcaacgg tggcatccgc acctgtcaga atcgcgccgc cttgcgcgcc tttttcgcgc
     1921 aacagcatgg tcaggcggcg ggtgtcgatg tcggcgatgg cgacggtttc gttgcgtacc
     1981 aaatagtcgt gcaggctttc ggaggcgcgg aagctgctgt gcaagagcgg caggtcgcgg
     2041 ataatcaggc ctgcggcgta aacgctgcgg ctttcttcat cttcggcgtt ggtgccggtg
     2101 ttgccgatgt gggggtaggt gagggtaacg atttgtttgc agtaggacgg gtcggtcaga
     2161 atttcctgat agccggtcat cgaagtattg aacacgactt cgccggaagc cgaaccttcg
     2221 taaccgattg atgtgccgtg gaatacgctg ccgtcagcga ggacgaggag ggcgggggtg
     2281 ctcatgatgg gaatcctgtt tttaataaaa ttcttgacaa tgccgtctga agggacttcg
     2341 gcagggggca tccggtcgga aaaatccggt caaaaaaaaa cacgccgcgc aagatagacg
     2401 cgtaacgtgc ttttcgggca agtgcctata ccctgaaagc agcgtattct aagacgaaac
     2461 ctacacgatt tcaagcgcaa acgcggcagg cgggctgata tgttcgcgcg aaaacggaaa
     2521 tccggggcat aaaaccgccc gctccgcgcc ccctatgccc gttacgcatt tccccacgtc
     2581 cgcccggcga aacta
//
LOCUS       DAXDXW010000130         2474 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXDXW010000130 DAXDXW010000000
VERSION     DAXDXW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.131"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..662
                     /locus_tag="ACFB64_002075"
     CDS             <1..662
                     /locus_tag="ACFB64_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC1210350.1"
                     /translation="ERITHDYPKPTGTGKNKISTVSDYFRNIRTHSIHPRVSVGYDFG
                     GWRIAADYARYRKWNDNKYSVNIENVKKHDNGNRIDRKTENQENGSFHAVSSLGLSAV
                     YDFKLNDKFKPYIGARVAYGHVRHSIDSTKKTIEFLTAPSNAPNGASTTYNISPQTQN
                     AHHQSDSIRRVGLGVIAGVGFDITPKLTLDAGYRYHNWGRLENTRFKTHEASLGMRYR
                     F"
     gene            676..1028
                     /locus_tag="ACFB64_002076"
                     /pseudo
     CDS             676..1028
                     /locus_tag="ACFB64_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360925.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1117..1686
                     /gene="rsmD"
                     /locus_tag="ACFB64_002077"
     CDS             1117..1686
                     /gene="rsmD"
                     /locus_tag="ACFB64_002077"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC1210351.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFASADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGMEAASRNAKRVVIADNNRQTVQTLEKNSRELGLG
                     QVQTVCSDGIAYLANLKEKFDVVFLDPPFAWQSWESLFNVLGTRLNDGAYVYIEAGRQ
                     PDKPDWLTGYREGKSGQGTFELRVFQVAE"
     gene            1809..2060
                     /locus_tag="ACFB64_002078"
     CDS             1809..2060
                     /locus_tag="ACFB64_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC1210352.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            2157..2240
                     /locus_tag="ACFB64_002079"
     tRNA            2157..2240
                     /locus_tag="ACFB64_002079"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2191..2193,aa:Tyr,seq:gta)
     gene            2271..2344
                     /locus_tag="ACFB64_002080"
     tRNA            2271..2344
                     /locus_tag="ACFB64_002080"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2303..2305,aa:Gly,seq:tcc)
     gene            2354..2428
                     /locus_tag="ACFB64_002081"
     tRNA            2354..2428
                     /locus_tag="ACFB64_002081"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2386..2388,aa:Thr,seq:ggt)
BASE COUNT          693 a          615 c          618 g          548 t
ORIGIN      
        1 ccgaacgcat tacccacgat tatccgaaac caaccggtac aggcaaaaac aaaataagca
       61 cggtaagcga ttatttcaga aacatccgta cgcattccat ccacccccgg gtgtcggtcg
      121 gctacgactt cggcggctgg aggatagcgg cagattatgc ccgttacaga aagtggaacg
      181 acaataaata ttccgttaac atagaaaatg tgaaaaaaca cgacaatggc aacaggatag
      241 accggaagac ggaaaatcag gaaaacggca gcttccacgc cgtctcctcg ctcggtttgt
      301 ccgccgttta cgacttcaaa ctcaacgata aattcaaacc ctatatcggt gcgcgcgtcg
      361 cctacggaca cgtcagacac agcatcgatt cgaccaaaaa aacaatagag tttcttaccg
      421 ccccctccaa tgctcctaac ggagcatcta caacttataa tattagtcca cagacgcaaa
      481 acgcccatca ccaaagcgac agcatccgcc gcgtaggtct tggcgtcatc gccggtgtcg
      541 gtttcgacat cacgcccaag ctgaccttgg acgccggcta ccgctaccac aactggggac
      601 gcttggaaaa cacccgcttc aaaacccacg aagcctcgtt gggcatgcgc taccgcttct
      661 gattcgccga ttccgatgcc gtctgaacct tcagacggca tttttgacgc gcccgccgtt
      721 tacaggcgcg gggcgggcgc agtaaaatac ccgaaccgcc attgccgata caccgtaacc
      781 ctaaaacccg ccattcccgc gcaggcggga atccggattt gtcgggattc gctcaaatcg
      841 ggcggcggcg ggatggtgtt gcagattaac gatgtggata ccaacagcgg ggaatttgca
      901 atctataccg ctcaggatgc atcggtaaag ctggatttgt taaaaatgga attgaaacac
      961 tgcaaagaaa tgttggaaca aaagacaaag aaatcgagtt gctccgcaag ctgaccgaaa
     1021 ccgtctaaac agatatgccg tctgaaaaaa gttttcaggc ggcatattct tcgacaggtc
     1081 ttgtataata ccgtttgaac tttcaggctt ttgattatgg cggcaggcaa acataccaaa
     1141 cacagcaacc gggtacgcat tatcggcggg caatgccggg gcaggaaatt gagttttgca
     1201 tccgccgacg gactgcgccc aacccccgac agcgtgcgtg aaaagctgtt taactggctg
     1261 ggacaggatt tgacgggaaa aacggttttg gatctcttcg gaggcagcgg cgcactcggc
     1321 atggaggcgg catcgcgtaa tgcaaaacgg gtcgtgattg cagacaacaa ccgtcaaacg
     1381 gtgcaaaccc ttgagaaaaa cagccgcgaa ctgggtttgg ggcaggtgca aaccgtctgt
     1441 tcggacggca ttgcctattt ggcaaaccta aaagagaaat tcgatgtcgt cttccttgat
     1501 ccgccgtttg cgtggcaaag ttgggaaagt cttttcaatg tattgggcac acgcctgaat
     1561 gacggcgcat atgtttatat cgaggcaggc aggcagccgg acaaacctga ttggctgacg
     1621 ggatataggg aggggaagtc ggggcagggt acatttgaat taagggtttt ccaagtggct
     1681 gaataatatg cgctttgata atcatttccg ggttgtaaac attcgtttgc aaccgtccgg
     1741 ttcaaaaaaa ccttgtgcta taatccgcgc ccgcccggtt ttgataattt agtggaaaag
     1801 gaaaagaaat gtcgcttttt attaccgacg agtgcatcaa ctgcgacgta tgcgaacccg
     1861 aatgccccaa tgatgccatt tcccaaggcg aggaaattta cgaaatcaac cccaacctct
     1921 gcacgcagtg cgtcggacac tacgatgagc cgcagtgcca gcaggtttgc ccggtggact
     1981 gcatcctgat tgacgaagaa catcccgaaa cccatgacga gttgatggcg aaatacgaaa
     2041 agattatcca gtttaaataa attcttttta aaacatcaaa ttatgtctgt tttgaaataa
     2101 aatcaaaaaa aaacttgacg gaaaagcaag ccgctaataa actagtgttc tcttttggag
     2161 ggattcccga gcggtcaaag ggggcagact gtaaatctgt tgcgaaagct tcgaaggttc
     2221 gaatccttct ccctccacca aaattcttac ttggggcagt agcgagtaat gcgggtgtag
     2281 ctcaatggta gagcagaagc cttccaagct tacggtgagg gttcgattcc cttcacccgc
     2341 tccaaacaat taggcccatg tagctcaggg gtagagcact cccttggtaa gggagaggtc
     2401 ggcagttcaa atctgcccat gggcaccatc tctcgattat tcatttcttt aaggcttaga
     2461 tatataggat attg
//
LOCUS       DAXDXW010000131         2297 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXDXW010000131 DAXDXW010000000
VERSION     DAXDXW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.132"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(94..1728)
                     /gene="groL"
                     /locus_tag="ACFB64_002082"
     CDS             complement(94..1728)
                     /gene="groL"
                     /locus_tag="ACFB64_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC1210353.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTVID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIGMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAVPDMGGMGGMGGMM"
     gene            complement(1821..2111)
                     /locus_tag="ACFB64_002083"
     CDS             complement(1821..2111)
                     /locus_tag="ACFB64_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC1210354.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT          425 a          626 c          618 g          628 t
ORIGIN      
        1 tttaaggttt gcgccttagc cgcacttatt cgcaaaaaaa ccgcacggcg ttgaccgtgc
       61 ggcttgttgt ctgaaggttt cagacggcat tgcttacatc atgccgccca taccgcccat
      121 tccccccata tcgggcacag ccggtttttc ttcagggatt tcggcaatca tgcagtcggt
      181 cgtcagcatc agaccggcga tagacgcggc gtgttgcagc gcggaacggg ttactttggc
      241 agggtcgagt acgcccattc cgatcatgtc gccgtattcg ccggagcctg cgttgtaacc
      301 gtagttgcct ttgccttcca acactttgtt caccaccacg ctgggttctc cgcctgcgtt
      361 ggcaacgatt tggcgcagcg gagactcaac ggcgcgcaat acgatttgta cgcctgcgtc
      421 ttggtcggca ttgccggtgt gcaggttttc caaagcggca cgggcgcgca acagggctac
      481 gccgccgcct gcaaccacgc cttcttcaac ggctgcgcgg gtagcgtgca gcgcgtcttc
      541 cacgcggtct tttttctctt tcatttcgac ttcggtcgcc gcgccgactt tgatcactgc
      601 cacgcctcct gccagtttgg caacgcgctc ttgcagtttt tctttgtcgt aatcgctggt
      661 cgcggtttcg atttgttggc ggatttcggc aacacgcgct tcgatttggg ctgcgtcgcc
      721 gaagccgtcg atgacggtag tgttttcttt accgatttcg atgcgtttgg cttgacccaa
      781 gtcgtccaaa gtcgcttttt ccaaagacag gccgacttct tcggaaatca ctacgccgcc
      841 ggtcaggatg gcgatgtctt gcagcatcgc tttgcggcgg tcgccgaagc cgggggcttt
      901 gacggcaacg gttttcagga tgccgcggat gttgttcacg accaaagtcg ccaaggcttc
      961 gccttctacg tcttcagcga taatcaacag cggacggctg gctttcgcca cttgttccaa
     1021 cacgggcagc aggtcgcgga tgttgctgat ttttttgtcg aacagcaaaa caaacggatt
     1081 gtccagaccg gcgatttgtt tttccgcgtc gttgatgaag taaggggaca ggtagccgcg
     1141 gtcgaactgc ataccttcaa ccacgtccag ctcgttttcc aaagatttgc cgtcttcaac
     1201 ggtaatcacg ccttctttgc cgactttttc catcgcttcg gcgataatcg cgccgacttg
     1261 ttcgtcggag ttggcggaaa tcgagccgac ttgggcgatt tctttggaag tatcgcaagg
     1321 tttggcgatg tttttcagct cgtcaaccaa agcggcaacg gctttgtcga tgccgcgttt
     1381 cagatcggtc gggttcatgc cggcggtaac gtatttcatg ccttcggcaa cgatggattg
     1441 tgccaatacg gtggcggtag tcgtaccgtc gccggctacg tcgttggttt tggacgcgac
     1501 ttctttcacc atttgcgcgc ccatattttc aaacttgtct ttcagttcga tttctttggc
     1561 gacggttacg ccgtctttgg tgatgtgcgg gccgccgaaa gcgcggtcaa ccaccacgtt
     1621 gcggcctttg gggcccaagg ttacgcgcac ggcgtttgcc aaaatattca cgccgtttac
     1681 cattttttgg cggacttcat tgccgaattg tacgtctttt gctgccattt caattctcca
     1741 aaaatcatta aaactgtctg ataaaaccgt ttatgccgtc tgaaggcggt ttgccgtttc
     1801 agacggcatc gtgtccgtat ttatttttca acgatgccga aaatatcttc ttcgcgcatt
     1861 accaacagct cttcgccgtc ggcttttacg gtttggccgc tgtatttgcc gaagatgatt
     1921 ttgtcgccga ctttgacatc cagcggacgg cgcgcgccgt ctttgccgat tttgcccgca
     1981 cccacggcga tgacttcgcc catatcgggt ttttcggcgg ccgcgcccgg caggacgatg
     2041 ccagaggcgg ttttttcttc agcttccaag cgtttgacga cgacgcggtc gtgtaaagga
     2101 cggatggtca tatttatgct ccgataaatg gtttgaaaac aatcatctgc ccgaacggtt
     2161 cggacaaatt gaagtggaaa ccgggctgcc gcctaacggc ctgcccgtac aagtcggcaa
     2221 aattagggtg tgtgcgggaa aattcaagtg aggcggaaaa aaatttattt ccgccgtttt
     2281 ttatagtgga ctaaatt
//
LOCUS       DAXDXW010000132         2280 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXDXW010000132 DAXDXW010000000
VERSION     DAXDXW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.133"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            172..366
                     /locus_tag="ACFB64_002084"
     CDS             172..366
                     /locus_tag="ACFB64_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC1210355.1"
                     /translation="MDSEYLDPQQCADILSVKKRTFLERYAPRPDFPARISVSKKRFW
                     WKKEEVEGWLDRQKEKRPVM"
     gene            complement(422..506)
                     /locus_tag="ACFB64_002085"
     tRNA            complement(422..506)
                     /locus_tag="ACFB64_002085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(470..472),aa:Leu,seq:tag)
     gene            657..1331
                     /locus_tag="ACFB64_002086"
     CDS             657..1331
                     /locus_tag="ACFB64_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210356.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGMASIDRSV
                     LWCNDEGWKLADYLPCDDVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENAALR
                     LVCLSQQGEGLENIWEQDGNITDVSLACRSAQSGWMNVASDVRRWLNLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECADTAAQAEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            1324..1917
                     /gene="ribA"
                     /locus_tag="ACFB64_002087"
     CDS             1324..1917
                     /gene="ribA"
                     /locus_tag="ACFB64_002087"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC1210357.1"
                     /translation="MSELLDHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNCSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQAEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQEQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENERYLQTKADKLGHLMSE"
     gene            2085..>2280
                     /locus_tag="ACFB64_002088"
     CDS             2085..>2280
                     /locus_tag="ACFB64_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC1210358.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIRVIPCVT"
BASE COUNT          555 a          524 c          650 g          551 t
ORIGIN      
        1 aactgcgcct attgcaacaa accgcctgcc gcttcaaagc cgccctcgaa acggccgcgt
       61 ggaaacacta cgtccgcgcc atcaaagaat ccgagccggt accggatgcc gaagcgcgcc
      121 ggaagcggaa aaaacaggcg gcgtaaatat aattaagttt ggaggtttta aatggatagc
      181 gaatacctag acccgcagca atgcgcagat attttatccg taaaaaagcg tacattttta
      241 gagcggtacg caccgcgtcc ggatttcccc gcgcgaatat ccgtatcgaa gaaacgtttt
      301 tggtggaaaa aagaagaagt cgaaggatgg ctagaccgcc aaaaagaaaa acgcccggtg
      361 atgtgatttg caccacattt gcaccaagtg gcatataaca ttatgaaaga tataaaaatt
      421 ttggtgcgga cggagagact cgaactctca cacctctcgg cgccagaacc taaatctggt
      481 gcgtctacca atttcgccac gtccgcataa gggtggagat tatacagatt ttgtccgatt
      541 gcgcaaggct ttggacgtaa tagttgaggc ttatgctttg cagcggtaaa atccgctatt
      601 cgtccgcctg gcatcggaat cgggcggttt tttgttttta ttgacggaat ttgggtatgc
      661 ctgctgcttt gattaaggat tttctgctga ctcagggttt gaagctgccg cttgacgagg
      721 ttcgggcggc gtatctgacg gcgcagacgg taatggatat ggggatggct tcgattgacc
      781 gttctgtttt gtggtgcaat gatgagggtt ggaaacttgc cgattacctg ccgtgcgatg
      841 atgtccgcga agatgccctg aaacggcttt ttatggcttt ggattcggtg ttttcacgct
      901 cgacaggtgt gcggagcgcg gcggtttatg ccttgatgcc gtctgaaaac gccgccctcc
      961 ggctggtgtg cctgtcccaa cagggcgaag gtttggaaaa catatgggag caggatggaa
     1021 atattaccga tgtttcgctt gcctgccgtt cggcgcaaag cggttggatg aatgttgcct
     1081 cggatgtacg ccgttggttg aatttgggtg agctttcggg agaacgcaat catgcttcgg
     1141 cggcgcaaat ttccattccc gtttgcacgg agagcggcgg tgtattgggc gtggttcatg
     1201 tggaatttga atgcgcagag tgtgcggata cggcggcgca ggcggaatgg gtggctcttg
     1261 ccttggcttt gtccgaacct ttgaagcaac ttttgggcat cactgccgca gaaggagatg
     1321 aaaatgtctg aattattaga ccatgtcgct tcctgccgtc tgccgaccga atggggcgta
     1381 tttacgatgc acggttttga agaggcaaac gggcaggaac acgtcgcgct gaccgtcggc
     1441 aattgttcag acggcaatcc ggtgttgacg cgcatccact ccgaatgtct gacgggcgac
     1501 gcgctgttct cgagaaaatg cgactgcgga ccgcaacttg aagcggcaat gagggcggta
     1561 caggcagagg ggcgcggcat catcgtctat ctgcgtcagg aaggacgcgg catcgggctg
     1621 attaacaaaa tccgcgccta tcatctgcaa gaacaaggta tggataccgt tgaagccaat
     1681 ttggcactcg ggctgcccgt cgatgcccgc gatttccgtt tggcgcaatc tatctacgaa
     1741 tatctgggca tccgctcggt caaactgttg accaacaacc ccgaaaaaat ccaaaccctg
     1801 aaagatgcgg ggattaacgt ggtcgaacgc attcccctgc acgtcgggga aaatctggaa
     1861 aacgagcgtt atctccaaac caaagcagac aagctggggc atttgatgtc ggaataaggc
     1921 aaagttgcag ggaacgggca tcctgcgccg tctttcggga aacaggtttc catagcttga
     1981 taaagcaata agttttatca agttgcaggg tgcggatgca aacgcattgc gagcgcgggt
     2041 ttgaggcata cgcgcaaaca tcttaataca acgtattgat atttatgatt ttctctatca
     2101 tcgtccctat ttacaatgtg gaaaaatacc ttcgctgctg cgtggattcc gtgcttgccg
     2161 aaaattttgc cgattatgaa atgattttgg tcgatgacgg ttcgccggac ggctgcggga
     2221 agatttgcga cgaatatgca ggcaaatatc cgcatataag ggtaatccca tgcgtaacgc
//
LOCUS       DAXDXW010000133         2265 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXDXW010000133 DAXDXW010000000
VERSION     DAXDXW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.134"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            112..336
                     /locus_tag="ACFB64_002089"
     CDS             112..336
                     /locus_tag="ACFB64_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210359.1"
                     /translation="MFIFYTVNPEHVYFPKAYIMKVFKDKGYESQCITTVSFYICNPT
                     LKQKTENEAYEYGRLFVKELMHKECNRESL"
     gene            365..1651
                     /locus_tag="ACFB64_002090"
     CDS             365..1651
                     /locus_tag="ACFB64_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC1210360.1"
                     /translation="MADALADGGAAATAVGCPPRLIGGEQNKTPNPKGAEKTENQEFQ
                     FEYFSHFVSDGKGRFIEIPLRRGRDDGAFIDQITFTIHENSMTKVTGKGLVSDTEFVV
                     RYSELLEEILGFGITKKLPFKGKFFYQSCYQFGPDNVEYGKVHYGGQCETMLVELNGT
                     GCMAALPGWENRLYEFLSKCVRPKITRIDVAHDFFNGEYTPDQAMLDHDNGHYDVHNM
                     RPKSECRGTAWRNEDGSGKTFYIGKRENSKFTRVYEKGRQFGDVDSPWVRFEGDIEIP
                     LDVLLYSGSYLGGAYPICKEIFKTEAKRMEVKVKNVNLIFDVKLFHARNQVGKMVNFL
                     RDIGWDDSRIVDELVKGVEGYPKGLQPEQYDCKNQTQKFQYIHEEQKAINALNIETLF
                     DDLIEEREYAFPQDRERQHIKDIEYEERQISDFLNN"
     gene            1665..1976
                     /locus_tag="ACFB64_002091"
     CDS             1665..1976
                     /locus_tag="ACFB64_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210361.1"
                     /translation="MFETSQVTTYTATLLGAKKFKGEIDGNKIDSCTVLVASPMPSNG
                     NAVGFTSESMKFGDSHNFEKLKNLKFPCAVDLTVAMESTGKGLVQKLLDFQVKGAAPK
                     A"
     gene            1990..2193
                     /locus_tag="ACFB64_002092"
     CDS             1990..2193
                     /locus_tag="ACFB64_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210362.1"
                     /translation="MSRYQQKFIVQELENHEFIYPDSFGDIGFTSNIKSAGKYDSYED
                     AFSSALEEIGGEFVIFGFYEKED"
BASE COUNT          736 a          444 c          534 g          551 t
ORIGIN      
        1 ggagcggata aattctcgca atctaaaacc ttatatattg cgctccataa ggggcgcata
       61 tttttaatcc ttgtaaagcc ccttaactct agtcagtgaa ggggcttaat catgtttatt
      121 ttctatactg tcaatcccga acacgtatat ttccccaaag catacatcat gaaagtgttc
      181 aaggataaag gctacgaatc ccaatgcatc acaacagttt ctttttatat ctgcaatccg
      241 actttgaagc aaaagaccga aaacgaagcg tatgaatacg gcagattgtt tgttaaagaa
      301 ctgatgcata aggaatgcaa ccgtgaaagc ctttgaagat aaagccttgg cggtgtccgc
      361 ggacatggcg gacgcgttag cggacggcgg ggctgcggca accgccgtag gctgcccccc
      421 taggctaata gggggggagc aaaataaaac cccaaatccg aagggtgcgg aaaaaacaga
      481 aaaccaagaa tttcaattcg agtatttcag tcattttgta tcggacggca aaggcagatt
      541 tatagaaata ccgttaaggc gtggcaggga tgacggagcg ttcatagacc aaatcacttt
      601 cacgatacat gaaaattcca tgacgaaagt aaccggaaag ggattggtat cagatacgga
      661 attcgttgtc cggtatagcg agctgcttga agaaatcttg ggattcggca ttaccaaaaa
      721 attaccgttt aaaggaaagt ttttctatca aagctgttat caattcgggc ccgataacgt
      781 cgaatacgga aaggttcatt acggcggcca atgtgaaacg atgttggttg aattgaacgg
      841 tacaggctgc atggctgctt tacccggttg ggaaaaccga ttgtatgaat ttttaagtaa
      901 atgcgtccga cccaaaataa cccgtatcga tgtcgcccat gattttttca acggagagta
      961 cacacccgat caggcaatgt tagaccacga taacggacat tacgacgtac acaatatgcg
     1021 gccaaaaagc gaatgtcgcg gcacggcatg gcgcaatgaa gacggcagtg gaaaaacttt
     1081 ttacatcggc aagcgcgaaa attcaaaatt tacccgtgtt tacgagaaag gcagacagtt
     1141 tggagatgtt gatagccctt gggtcaggtt tgagggggat atagaaatac ccttggatgt
     1201 cctactttat tcggggtcgt atctaggcgg cgcatatccg atttgcaagg agatttttaa
     1261 gacagaagcc aagcgcatgg aagtaaaagt taaaaacgtt aatctgatat tcgacgttaa
     1321 gctttttcat gcaaggaatc aagtcggcaa gatggttaat ttcctccgcg atatagggtg
     1381 ggacgatagc aggatagttg atgagctggt gaaaggcgtt gaaggttatc caaaaggttt
     1441 acagcctgag caatacgatt gcaaaaacca aacgcagaaa ttccagtata tccacgaaga
     1501 acagaaagcc attaacgcat tgaacattga aacactgttt gatgatttga ttgaagagag
     1561 agaatacgca tttcctcaag acagggaacg gcaacacatc aaggatattg agtacgagga
     1621 acgtcaaata tccgattttt taaacaactg aaggaaattg aaaaatgttt gaaacaagcc
     1681 aagtaaccac atataccgcc actttgttgg gcgcaaaaaa attcaaaggc gaaatcgacg
     1741 gtaacaaaat cgattcatgc accgtcttgg tagccagccc catgccgtca aacggcaatg
     1801 ccgtgggctt tacctcggaa agcatgaagt ttggtgacag ccataatttt gaaaaattga
     1861 aaaaccttaa attcccctgt gcggttgatc taaccgttgc catggaatca acaggaaaag
     1921 gcttagtgca gaaattgttg gatttccaag taaagggcgc agcacctaaa gcctaagaaa
     1981 ggctgaatca tgagcagata ccaacagaaa tttatcgtac aggaattgga aaatcacgaa
     2041 ttcatctatc ccgattcgtt tggcgatatt ggatttacgt cgaacatcaa atcagccggt
     2101 aaatatgaca gctatgaaga cgcttttagc tcggcattgg aagagatagg cggggaattt
     2161 gtaattttcg gattctacga aaaagaagat taagtttaag aggctcggca ggcggtctct
     2221 aaaaccttca caaagcccgc aaacacattt ttttaaaaat ttcgt
//
LOCUS       DAXDXW010000134         1929 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXDXW010000134 DAXDXW010000000
VERSION     DAXDXW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.135"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(258..1538)
                     /gene="hemL"
                     /locus_tag="ACFB64_002093"
     CDS             complement(258..1538)
                     /gene="hemL"
                     /locus_tag="ACFB64_002093"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC1210363.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAEI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGDEIACVIVEPFVGNMNLVRPTEA
                     FVKALRGLTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTALTQRLANG
                     IAAAKAHGIEFAADSVGGMFGLYFAAHVPRNYADMARSNIDAFKRFFHGMLDRGIAFG
                     PSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1602..>1929)
                     /locus_tag="ACFB64_002094"
     CDS             complement(1602..>1929)
                     /locus_tag="ACFB64_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC1210364.1"
                     /translation="TEEGIAQTVVKGKTTRAEVEARFGKRNPFGCYAYHEVSLPIYNF
                     LPTNFIYMKSERRHWEWCADYDGEGVVRDYRFTHKVEKDERSVIRDTVGVIRKEAGKS
                     LSQPEK"
BASE COUNT          422 a          537 c          531 g          439 t
ORIGIN      
        1 ctactgtcct attttgattt taattgacta tatatttcag acggcatttt tacagtaaat
       61 tgaaatcaaa aatgtacacg cccgtcattc ccgcgcaggc gggaatccgg aaggtcgggc
      121 ttgccgttat tttcaatcat tacagaaact gaaaggtccg gattcccgcc tgcgcggaaa
      181 tgacgaatga cggcggtgtg cattcttact tcaatctgcc aaacaaatcg aacagagaaa
      241 ctctgtccgt caaaacatca tgcagccatc gccttgaaca cttcaaccgc aaccgcaacc
      301 gtttcgtcaa tcagctcggg cgtatgcgcg gcggaaacga aacctgcttc ataagcggac
      361 gggccgaagg caatgccgcg gtcgagcatg ccgtggaaga agcgtttgaa agcgtcgata
      421 ttggagcgcg ccatatcggc atagtttcgc ggcacgtgtg cggcgaaata cagaccgaac
      481 ataccgccca cgctgtcggc ggcaaactcg ataccgtgcg ctttggcggc ggcaataccg
      541 ttggcaaggc gttgtgtcaa ggcggtcagg ttttcataga agccttcgcg ctggatgatt
      601 tccagcgttt tcaagccggc ggcgacggca atcgggttgc ctgataatgt acctgcctga
      661 tacacgccgc ccaacgggga aatacattcc atgatgtctt tgcgtccgcc gaacgcggca
      721 agcggcatac cgccgccgat gactttgccc atcgtggtca ggtcgggcgt gatgccgtgc
      781 agcgactgcg cgccgccgag cgcgacgcgg aaaccggtca tcacttcgtc gtaaatcaac
      841 accgcgccgt gtttttcggt caatccgcgc aaggctttga caaaggcttc ggtcgggcgg
      901 acgaggttca tattgccgac gaagggttcg acaatcacgc aggcgatttc gtcgccgctt
      961 tgggcaaagg cttcttcgag ttgggcgatg ttgttgtatt cgagtaccaa agtatgtttg
     1021 gtaaagtcgg caggcacacc ggcggaagaa ggattgccga aggtaagcag accgctgcct
     1081 gctttcacca acaggctgtc ggaatggccg tggtagcagc cttcaaattt gatgattttg
     1141 tcgcggccgg taaaaccgcg tgccagacgg atggcagtca tcgtcgcttc cgtgccggag
     1201 ctgacgaggc gcagccgttc gacagacggc ataatttcgg caatttgttc ggcaatggcg
     1261 atttcgcctt cggtgggcgc gccgaacgac aaaccgccca acgcagcttc gcgcacggct
     1321 tcgacgactt cgggatgcgc gtgtccgaca atcgcaggcc cccaagagcc gacataatcg
     1381 gtgtagcgcg tgccgttttc gtcccaaaca tacgcgcctt cggctttttt gatgaagcgc
     1441 ggcacgccgc cgacgctgcc gaatgcgcgc acgggcgaat tcacgccgcc ggggatgatg
     1501 gctttggcgc ggtcgaataa aatttcgtta cggttcatat ttatcctcaa atgccgtctg
     1561 aacggcaggt ttcgggcttg gaagcagaaa gccccatttt atcatttttc aggttgcgac
     1621 aaggatttgc ccgcttcttt gcggattacg ccaaccgtat cccggatgac ggaacgctcg
     1681 tctttttcca ctttatgtgt aaagcggtag tcccggacga ctccctcccc gtcgtaatcc
     1741 gcgcaccatt cccaatgccg ccgctccgat ttcatataga tgaaattggt cggcaaaaaa
     1801 ttataaatcg gcaggctgac ctcgtgatag gcataacaac cgaaagggtt gcgcttcccg
     1861 aaacgtgcct ctacctccgc ccgggtcgtt ttgcctttaa caaccgtttg tgcgattccc
     1921 tcttccgtc
//
LOCUS       DAXDXW010000135         1776 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXDXW010000135 DAXDXW010000000
VERSION     DAXDXW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.136"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..282
                     /locus_tag="ACFB64_002095"
     CDS             <1..282
                     /locus_tag="ACFB64_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC1210365.1"
                     /translation="VWVHNDCPPKPKPTNHAQQRKEEAKNDSHRSVGDSNRVVREGKQ
                     YLDSDTGNHVYVKGDKVVILTPDGRQVTQFKNSKANTSKRVKNGKWTPK"
     gene            260..541
                     /locus_tag="ACFB64_002096"
     CDS             260..541
                     /locus_tag="ACFB64_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210366.1"
                     /translation="MGNGHQNNQSSLEKRIFYLEHSGQYLMICALSDYSQNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEKAISHHLEDFS"
     gene            597..1244
                     /locus_tag="ACFB64_002097"
                     /pseudo
     CDS             597..1244
                     /locus_tag="ACFB64_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147002.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            1241..1657
                     /locus_tag="ACFB64_002098"
     CDS             1241..1657
                     /locus_tag="ACFB64_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210367.1"
                     /translation="MKTSTIVFGGFFITDNGERIQIPILENPNIKEINNFFSVSNFEK
                     KAGVLVFRIIPEPEFGNTELTIYFEKGYYLPIIQTILEDGDIEVKNLKTENYSGNTME
                     ILGDVYPIEHISKNISIIQDIISEFIMKNKPITIMI"
BASE COUNT          640 a          306 c          334 g          496 t
ORIGIN      
        1 gtttgggttc ataatgattg tccgcctaaa ccaaaaccaa ccaatcatgc ccaacaaaga
       61 aaagaagaag ctaaaaacga ttctcatcga agtgtgggag attccaatcg tgtcgttcgc
      121 gaaggaaagc aatatttaga ttccgacaca ggaaaccatg tttatgtaaa aggagataaa
      181 gtggttattc taactcctga tggaagacag gtaactcaat ttaagaactc gaaagccaat
      241 acgtcaaaaa gggtaaaaaa tgggaaatgg acaccaaaat aatcaatctt ccttagaaaa
      301 aagaattttt tatctggagc attcggggca gtatctgatg atttgcgccc tatcggatta
      361 ctcccaaaat aaacatactg tcgttatggc gaatttcctc tatccaaatg aaaaaatgga
      421 ttggagaaat ttagatgatt tgttcaatga gttggtttta gaggaattac agtcttcatt
      481 tatggattgg tatccaacta ttgaaaaggc aattagccat catttagaag acttttcgta
      541 acaagcataa atcaaaaagt cgtctgaaac tgcttttaat tttcagacga cctttcccca
      601 tatcggcgga ttatgaattt acagctatcg ggaaattgag ttatctctct gatagagaaa
      661 attccatacg cgaagccgtt gaccggtgga tacaggaaaa tcccaatgcc gccgaaaccg
      721 tcgaagccgt cttcaacgtt gccgccgcca aagtcgcgaa gctggcaaag gcggcgaaac
      781 cggggaaagc tgcggttagc ggggattttt cgattagcta taaaaacttt tctactgtca
      841 aaccaaaagt aattgccaaa ggaacgatta atggaaagac ttttagagat gttaatcaaa
      901 gtgcaaaaat tggatcgcct gattcaccta cattaatagc ccaacgagtt aatgcaaaaa
      961 tccaagccga cggtaaacct cgtccgaatg caaccgttgc taattcacat gctgaaattg
     1021 gtgttatcca gcaggcttat aatgcaggtg aaacgaaagg ggcatctatg acaatgacag
     1081 tttcaggaaa agatgtttgc ggttattgta aaggagatat tgcagctgct gctcaagctt
     1141 ctggactgaa atcactgact gttaatgcga cagataatgt aacaggaaaa aataaaactt
     1201 actattggac gcctggtatg aaatcaatta aggagagaaa atgaaaacat caacaattgt
     1261 ttttggtgga ttttttatta cagataatgg agaacgaatc caaatcccta ttttagaaaa
     1321 tcctaacatt aaagaaataa ataacttttt ttctgtatca aattttgaaa aaaaagccgg
     1381 agtccttgtt ttcagaatta ttcctgagcc tgaatttggc aatactgaat taactatcta
     1441 ttttgaaaaa ggatattatt taccaataat tcaaacaatt ttagaagatg gagatattga
     1501 agtaaaaaat cttaaaacag aaaattatag tggaaataca atggaaattt tgggagacgt
     1561 ctaccctatt gagcatatct ctaaaaatat ttctataatt caagatataa tttctgaatt
     1621 tattatgaaa aataaaccaa tcacaataat gatttaattg aaaagttcaa caatcaaaag
     1681 gtcgcctgaa atcgtgttta attttcagac gacctatttc cttcatttga aacaggatat
     1741 tgaaaactaa gttcttcaaa aatcctacac ctgccc
//
LOCUS       DAXDXW010000136         1773 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXDXW010000136 DAXDXW010000000
VERSION     DAXDXW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.137"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            35..976
                     /gene="mafA"
                     /locus_tag="ACFB64_002099"
     CDS             35..976
                     /gene="mafA"
                     /locus_tag="ACFB64_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC1210368.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPDSATRYSYPA
                     YDTTATTKSDALSGVTTSTSVLNAPAAALTKNNGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            980..>1773
                     /gene="mafB"
                     /locus_tag="ACFB64_002100"
     CDS             980..>1773
                     /gene="mafB"
                     /locus_tag="ACFB64_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC1210369.1"
                     /translation="MKPLRRLIKLLAACAVAAAALIQPALAADLAQDPFITDNTQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASEEKGNVDDGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRRGNSMEFVNGVAAGALNPFISAGEALGIGDILYGTRYAIDKAAMR"
BASE COUNT          466 a          662 c          395 g          250 t
ORIGIN      
        1 actcaaaccc gttcaaaaca aaacaaaccc tgccatgaaa accctgctcc tcctcatccc
       61 cctcgtactc accgcctgcg gcacactgac cggcataccc gcccacggcg gcggcaaacg
      121 ctttgccgtc gaacaggaac tcgtcgccgc atcgtcccgc gccgccgtca aagaaatgga
      181 cttgtccgcc ctgaaaggac gcaaagccgc cctttacgtc tccgttatgg gcgaccaagg
      241 ttcgggcaac ataagcggcg gacgctactc catcgacgca ctgatacgcg gcggctacca
      301 caacaacccc gacagcgcca cccgatacag ctaccccgcc tatgacacta ccgccaccac
      361 caaatccgac gcgctctccg gcgtaaccac ttccacatcg gtcttaaacg cccccgccgc
      421 cgccctgacg aaaaacaacg gacgcaaagg cgaacgctcc gccggactgt ccgtcaacgg
      481 cacgggcgac taccgcaacg aaaccctgct cgccaacccc cgcgacgttt ccttcctgac
      541 caacctcatc caaaccgtct tctacctgcg cggcatcgaa gtcgtaccgc ccgaatacgc
      601 cgacaccgac gtattcgtaa ccgtcgacgt attcggcacc gtccgcagcc gtaccgaact
      661 gcacctctac aacgccgaaa cccttaaagc ccaaaccaag ctcgaatatt tcgccgtcga
      721 ccgcgacagc cggaaactgc tgattgcccc taaaaccgcc gcctacgaat cccaatacca
      781 agaacaatac gccctctgga tgggacctta cagcgtcggc aaaaccgtca aagcctcaga
      841 ccgcctgatg gtcgatttct ccgacatcac cccctacggc gacacgaccg cccaaaaccg
      901 tcccgacttc aaacaaaaca acggtaaaaa ccccgatgtc ggcaacgaag tcatccgccg
      961 ccgcaaagga ggataaaccg tgaaaccgct gcgcagactc atcaagctcc ttgccgcctg
     1021 tgccgtagcg gcggccgcac tcatacagcc cgccctcgcg gcggacttgg cgcaagaccc
     1081 gttcattacc gataacaccc aacggcagca ctacgaaccc ggcggcaaat accacctctt
     1141 cggcgacccg cgcggcagcg tttccgaccg caccggcaaa atcaacgtca tccaagacta
     1201 tacccaccag atgggcaacc tgcttatcca acaggcaaac atcaacggca caatcggcta
     1261 ccacacccgc ttttccggac acggacacga agaacacgcc cccttcgaca accacgccgc
     1321 cgacagcgca agcgaagaaa aaggcaacgt tgacgacggc tttaccgtgt accggctcaa
     1381 ctgggaagga cacgaacatc atcccgccga tgcctacgac ggcccgaagg gcggcaatta
     1441 ccccaaacct acgggcgcac gagacgaata cacctatcac gtcaacggca cagcccgcag
     1501 tatcaaactc aatccgaccg acacccgcag catccggcaa cgcatatccg acaactacaa
     1561 caacctcggc agcaatttct ccgaccgcgc cgatgaagcc aacagaaaaa tgttcgagca
     1621 caatgccaag ctcgaccgcc ggggcaacag catggagttt gtcaacggcg tcgccgccgg
     1681 cgcgctcaac ccctttatca gcgcgggcga agccttgggc ataggcgaca tactgtacgg
     1741 aacgcgctat gccatagaca aagccgcaat gcg
//
LOCUS       DAXDXW010000137         1758 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXDXW010000137 DAXDXW010000000
VERSION     DAXDXW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.138"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..935
                     /gene="mafB"
                     /locus_tag="ACFB64_002101"
     CDS             <1..935
                     /gene="mafB"
                     /locus_tag="ACFB64_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC1210370.1"
                     /translation="SRADEAGKLIWENNPDKNWWGNRMDDVRGIIQGAANPFLTGFQG
                     LGVGAITDSAVNPVTYAAAQQTLQGIHDLGNVSPAAQLAAASLLQDSAFAVKDSINSA
                     KQWADAHPNITATAQTALAVAEAAATVWRGKKVKLNPTKWDWVKNTGYKKPAARPMQT
                     VDGEMAGKNKPPKPSTQQHSTHSDNNIGLPAPYVKPDTSISPTGTIQDRIRWTKSKFP
                     TEKSLNGHFKAHGKEFGDITIEDYQKMASDLLSKQTSDKILGYQTEHRRVRYDINNNI
                     YVLANPKTFKIKTMFKPDLGKEYYDGEFKKDMGN"
     gene            901..1107
                     /locus_tag="ACFB64_002102"
     CDS             901..1107
                     /locus_tag="ACFB64_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HFC1210371.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            1128..1487
                     /locus_tag="ACFB64_002103"
     CDS             1128..1487
                     /locus_tag="ACFB64_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210372.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLDRI
                     TTNECINGIKKSIKKTN"
BASE COUNT          629 a          321 c          352 g          456 t
ORIGIN      
        1 tcagccgtgc tgatgaagca ggaaaactga tatgggaaaa caaccccgat aaaaattggt
       61 ggggtaaccg tatggatgat gttcgcggca tcatccaagg tgcagccaat ccttttctaa
      121 cgggttttca gggattggga gttggggcga ttacagacag cgcggtaaac ccggtaacct
      181 atgcggcagc acagcaaact ttacagggta ttcacgattt aggaaatgta agtcccgcag
      241 cacaacttgc ggccgcgagc ctattacaag acagtgcttt tgcggttaag gacagtatca
      301 attccgccaa gcaatgggct gatgcccatc cgaacattac agctactgcc caaactgccc
      361 ttgccgtagc agaggccgca gctacggttt ggagaggtaa aaaagtaaaa cttaacccga
      421 ccaaatggga ttgggttaaa aataccggct ataaaaaacc tgctgcccga cctatgcaga
      481 ctgtagacgg ggaaatggcc gggaaaaata agccaccgaa accaagtacg cagcaacact
      541 ctacacactc tgataacaat atcggcttac ctgccccata tgttaaacct gatacatcta
      601 tttctccgac aggaacaatt caagaccgca tcagatggac aaaatccaag tttcctactg
      661 agaaatcttt aaatggacat ttcaaagctc atggaaaaga atttggcgat ataaccattg
      721 aagactacca aaaaatggcg tctgatttgt tatcaaaaca gacatcggac aagatattag
      781 gttatcagac ggaacataga cgagtgcgct atgatatcaa taacaatatc tatgttttgg
      841 ccaatccaaa aacattcaaa atcaaaacaa tgtttaaacc agacttagga aaggagtatt
      901 atgatggaga attcaaaaaa gacatgggaa attgacggag aaatatggct acattgtcct
      961 gtttgcggaa ctgaagttat ggactatgat atctgtgacg tttgtcagtg gcaaaataca
     1021 ggagaaacta atatagatgg tggtcctaat gaaatgacac ttgcggaggc gaaagaagct
     1081 tacgcaaaag gcttaccaat cagataaata agcacctaga gaaaccaatg atgacggaac
     1141 cccatggtta cccatcaaat aatttacctg ttaaaatcat aaatgatatt atttatgcaa
     1201 cccagttagt cgaagatttg gttttaggaa aaataaaaat tgttgatttt ttaaaatcat
     1261 ataacaattt ttattattgg cttggttttg atgagttacc tcaatctgag aaaataaaat
     1321 ttctaaacta tctcaatata ttaagtattc ataaggaaat acaagataaa actgtgaata
     1381 gggtttatac cgattgtttt gatattgata aattacattc gcttgacaga attacaacta
     1441 atgaatgtat taatggtata aaaaaatcta tcaaaaaaac aaattagaat ttgacaatat
     1501 actcaaataa ctagcctatg ttttgtaaaa taaaaatatt cttgctgagt gtagagccag
     1561 agataaaaag atttgatagt agtttgcagt tgtagaatta tataaacaag aaaatagtca
     1621 gcagccgtag gtcggattct cgaatccgac attttccaac agcggcattt cggaaacgat
     1681 agatgcgtca aatatttttg tcggatacaa atatccgacc tacatctctg cgcagcaaac
     1741 tttacaagat attaatga
//
LOCUS       DAXDXW010000138         1729 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXDXW010000138 DAXDXW010000000
VERSION     DAXDXW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.139"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(1..83)
                     /locus_tag="ACFB64_002104"
                     /pseudo
     CDS             complement(1..83)
                     /locus_tag="ACFB64_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(101..445)
                     /locus_tag="ACFB64_002105"
     CDS             complement(101..445)
                     /locus_tag="ACFB64_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210373.1"
                     /translation="MNLENYENILIKLLFHHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDPLPD
                     NMIINNFFEIND"
     gene            complement(844..1245)
                     /locus_tag="ACFB64_002106"
     CDS             complement(844..1245)
                     /locus_tag="ACFB64_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210374.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINFDKVEVIAVGYRNTGKTIKISIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDERYAAWISKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIES"
     gene            complement(1249..>1729)
                     /locus_tag="ACFB64_002107"
     CDS             complement(1249..>1729)
                     /locus_tag="ACFB64_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210375.1"
                     /translation="GYKKPTARHMQTKALGTVDEIGNTVQQVGKQTSGQKTSGGNPAI
                     DSDPYSPSSVSARIEAGKVRSDLQIKDILSNTTQRSKTKGPAVQYDKVGDYNDALNDF
                     NSLNVRNVQTRPNGTITGNLPDGRAVNVRNDSSGGEPTLEITISNNRKIKIRYGNTR"
BASE COUNT          540 a          339 c          260 g          590 t
ORIGIN      
        1 tgaaaacccg ttaaaaaagg attaaccgca ccttggacga tgccgcgaat atcatccata
       61 cggttacccc accaattttt attggtttat tggtatatcg ttaatcattt atttcaaaaa
      121 agttatttat tatcatatta tcagggagcg ggtcgttaaa aaaaactccc tcaaattcca
      181 ttaaattagt atttatccag cacataaacg aaccaaagcg ttctttccct gagtgtaaat
      241 atttaaaatt aaactcaaaa taagtttttt tgcttagaaa atttaatggt ttttctatag
      301 tgaaaaaaaa gcccttactc gttttagttc gagatactat tttaaataag tttttattct
      361 caataaaata agaatattca tttactaagt tgttatgatg aaaaagtaat tttattaaaa
      421 tgttttcata attttctaga ttcatttcta gctcaattta aaatgatttc attgttgccg
      481 taccaatgcg aaatttacca tcaacaaaat ttcccttcac tgatacaggt accccatcgc
      541 agagatgtag gtcggatatt tgtatccgac aaaaatattt gacgcgttta tcgtttccga
      601 aatgccgctg ttagaaaatg tcggattcga gaatccaacc tacggctata tccgtaaagt
      661 ctgaccattg ttcaatgcgg aaggcgcgaa tattgcggac ggaatgtaga aaccaatata
      721 cttaattgcc atgccattct catcaaaata cctgtgctgt ttaaagtgcg tggttcatta
      781 cacgagtaat tttattttca ggctgcctga aagaaagata ggcaacttat tactcatctt
      841 acgctagctc tcaataattg aatacgttgc actagaacca attatttcta tgaatgtttg
      901 ctcaaacata aaaatatagt atttaaagaa ttctctattc aaaggaaaat aactttcttt
      961 cgatatccat gccgcataac gctcatcaag aagttcgaaa aaagattgac caccattttc
     1021 atgaagcaaa gctctatctg catcttccga aattaaatcc ataaaagtat ctataaaata
     1081 cgattcatca acaaccctaa aatgaataac atcctcaata gaaattttta tggttttacc
     1141 cgtatttcta taaccaactg ctataacttc tactttatca aagtttattg ctgcattatc
     1201 aaaatagaaa tagccagttg ggaaatctaa gctttttaat ttcataattt atcgtgtatt
     1261 tccatatctg atttttattt ttcggttatt actaattgtt atttcaagtg ttggttctcc
     1321 accactgcta tcattacgaa cattaaccgc acgcccatca ggtaaattgc ccgttatcgt
     1381 tccattagga cgtgtttgta catttcgaac attcagacta ttaaaatcat ttagtgcgtc
     1441 attgtaatcc cccactttat catactgaac agcgggacct tttgttttac tcctttgagt
     1501 agtattgctc aaaatgtctt tgatttgtaa atcactgcgc accttaccgg cttctatacg
     1561 agctgacaca ctactcgggc tataggggtc gctatcaatc gcaggattac caccgctggt
     1621 tttttgtccg ctagtctgtt tcccaacctg ctgtactgta ttgccaattt catctaccgt
     1681 acctaacgcc ttagtctgca tatggcgggc agtaggtttt ttataaccg
//
LOCUS       DAXDXW010000139         1708 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXDXW010000139 DAXDXW010000000
VERSION     DAXDXW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.140"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(208..1593)
                     /locus_tag="ACFB64_002108"
                     /pseudo
     CDS             complement(208..1593)
                     /locus_tag="ACFB64_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
BASE COUNT          485 a          521 c          313 g          389 t
ORIGIN      
        1 ccaccctgct tgtccgatgc ctcgggacgg acgggcaaac cgacacggca cgcaaaccgc
       61 gtcggcaaaa atgccgtctg aaaaaattca gacggcatct tcagacacat tacctgcaaa
      121 cggcaacaca ttccaatcca aaccgatcag gcaatcaata cgaacggctg tttacatact
      181 taccagcttt caccaaccgg tatcgattta accgatttcc ttaatatttt tcctgtccgt
      241 tttaaacttc gccttaaaca catccggtaa atctttatcg aaataccaaa gcccgtcatc
      301 catttccaat gcgccgccca ttccatgcag aacgactttt cccccgttaa aggatcggtt
      361 tcggtaattt ccacctcatg catttttccc caatccaaaa caggcaactt gtgtgcaaac
      421 gccaaaactt gttcgtcaaa acggccgcac gccttatcgc atcggcctga aacatatacg
      481 ggacaaggct catcctctgc ataaccgtcc ggaaggatac cggctgaggc aggacacaat
      541 ccgtatgttt ccgcccacga cgacaaagcc cgtctcgccg cctttttatt ggcaaataat
      601 ccggtaggcg gattatccgt cacaccgttt ttcaaagccg ctgttttcgc attcaacatg
      661 ccgtctgaac ctttttcaaa cctgacggtc gtaaatgttt taagcagatt tttggcagat
      721 acataacaac ccgaatgata acgtccgacc aattccgctt taatcttata tgcatgcagg
      781 ctgcccaatg cgggaaaaaa acggacttcc tccgtattgc accaatcaaa cgggggcttt
      841 tccggaatcc aacaaagccg caacctcgcc atatgcccgt tcaaacgtac cgatataatt
      901 tacttttccc ccgccgtcga aacaggcaag cacacctgta ccgtcgggca aaccgtacag
      961 ccgttccctc aaccgttcgg gaagcgcggc aggaagaagt ttcggattcg tcaaacggaa
     1021 acactgcctg ctccactcct caagcccgtg tgccgacagg ctgtattcca aataatcaca
     1081 caacgccgat acatccgcca tcgcacgatg cctgtcttcc acaacaatcc ccaacctttc
     1141 gatgataccg tccaggctgt gcttgtaaaa ctgcggatac agccgacgcg aaagctgcac
     1201 actgcacaaa gcaggcgatg aaaatccgat acccgcacga tgaaactcat gctttaaaaa
     1261 cgtatagtcg aaacggctgt tgtgcgccac cagcacgcac cccttcaata ccgaaaacaa
     1321 cccgccggca atttcagcaa acaccggcgc gtcggcaacc atgccgtctg aaatccccgt
     1381 cagttccgcc acaaacttcg gaatcggttt ttgaggattg accagccatt catacctctc
     1441 cgcccttccc tgcccaaact tgaccaaagc cacttcggtt accctgtctt catacagatt
     1501 gccgcccgtc gattccaaat caaccacggc aacaggcatt ccaaaccgta aaaacgcctt
     1561 ttccagcaag ggccagcggg aaacaacaat cattttattc tctttaaatt caaacaacaa
     1621 accgatattt tacactttta aggcatttca tccaacaaaa taattgacag aatccgatga
     1681 ttaccataaa attcaaaaat ttattgca
//
LOCUS       DAXDXW010000140         1665 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXDXW010000140 DAXDXW010000000
VERSION     DAXDXW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.141"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..879
                     /locus_tag="ACFB64_002109"
     CDS             <1..879
                     /locus_tag="ACFB64_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC1210376.1"
                     /translation="ETLAEDTGFAETAVRQHIKWLKDNNFIKSARRQRGRERKSDIYR
                     INVALLEKCYAEAAKRKAARQAKMWEEPLDYEPSDFEPSDYEPSDYEPSDFDAKNHQI
                     LSGEPSDFALRTVRFCAKNHQILSGEPSDFDGSLYVEPSVEPSVEPSGSNARGARAPA
                     GPHPAKPQTAPPETAPAAKAKKTGRHETELSLLADYGITGQVAADFLQVRKAKRQPLT
                     ETAMRLIAADAEKCGMTALQAAEYAIASGWGSFRADWLQNKTFGRSGNRGGPTHNQTA
                     AVPDAGSYGDMPTTDF"
     gene            891..>1665
                     /locus_tag="ACFB64_002110"
     CDS             891..>1665
                     /locus_tag="ACFB64_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC1210377.1"
                     /translation="MALKGASDFLGAYGGGVRVERRQCAEHGGYAAKSVLRGVWTGCP
                     ACRKLEAADEMAAYAETLRRGAMRDALEKRIGRSGIAPRFRNCRIENYAVSDSIPGMA
                     RAKAAAAEYAANFADVLQTGRSMIFSGRRGTGKNHLACGIAREVIAAGKSALVITVGD
                     MLRTVKDSFGGGGGEAGAVGIFVKPDLLVLDEFGAGSLSETDGRILFSVVNARYERLM
                     PMLVLTNLTAEAFRENTDARIRDRLRDGGGKLIPFDWESY"
BASE COUNT          357 a          459 c          582 g          267 t
ORIGIN      
        1 gaaacgctgg cggaagatac gggcttcgcc gaaaccgccg tacggcagca tatcaagtgg
       61 ctgaaggata acaatttcat caagtccgcc cggcggcaga gggggcggga gaggaagtcc
      121 gacatctacc gcatcaacgt cgccctgctt gaaaaatgct atgcggaggc ggcaaaacgg
      181 aaggcggcgc ggcaggcaaa aatgtgggaa gaaccattgg attacgaacc ttcggatttt
      241 gaaccgtcgg attacgaacc gtcggattac gaaccgtcgg attttgacgc taagaaccat
      301 cagattttga gcggcgaacc gtcggatttt gcgctaagaa ccgtcagatt ttgcgctaag
      361 aaccatcaga ttttgagcgg tgaaccatca gattttgacg gttccttata tgtagaaccg
      421 tcagtagaac cgtcagtaga accgtcagga tcaaatgcgc gcggcgcgcg cgcccctgcc
      481 ggaccgcacc ctgcgaaacc gcaaacggcg cctcccgaaa ccgcaccggc ggcgaaggcg
      541 aaaaaaaccg gcaggcacga aaccgagctt tcgctgcttg ccgactacgg catcacgggg
      601 caggtggcgg cggacttcct gcaagtccgc aaggcaaaac ggcagccgct gacggaaacg
      661 gcaatgcgcc tgattgccgc cgatgcggag aaatgcggga tgacggcgct gcaggcggcg
      721 gagtacgcca tcgccagcgg ctggggcagc ttccgcgccg actggctgca aaacaaaact
      781 ttcggcaggt ccggaaaccg cggcggcccg acgcacaacc aaaccgccgc cgtgccggat
      841 gcgggaagct acggcgatat gccgacgacg gatttttgag ggggcttggt atggctttga
      901 agggcgcgtc tgattttttg ggggcttacg gcggcggcgt gcgggtcgag cggaggcaat
      961 gcgcggaaca cggcggatac gcggcgaaaa gcgttttgcg cggcgtgtgg acgggctgcc
     1021 cggcctgccg gaagctggag gcggcggacg aaatggcggc atacgcggaa acgctgcgcc
     1081 gcggggcgat gcgcgacgcg ctggaaaaac gcatcgggcg ttcgggcatc gccccgcggt
     1141 tcagaaactg ccggattgaa aactacgccg tcagcgattc gatcccgggg atggccaggg
     1201 cgaaggcggc cgccgccgag tatgcggcaa acttcgccga tgtgttgcag acggggcgga
     1261 gcatgatttt ttcgggcagg aggggcacgg gcaaaaacca ccttgcctgc ggcatcgccc
     1321 gcgaagtcat cgccgccggc aaaagcgcgc tggtcatcac ggtgggcgat atgctgcgga
     1381 cggtcaagga cagtttcggc ggcggcggcg gcgaggcggg ggcggtcggg attttcgtga
     1441 agcccgattt gctggtgctg gacgagttcg gcgcgggcag tctgtcggaa acggacgggc
     1501 ggattttgtt ttccgtcgtc aacgcccgct atgagcggct gatgccgatg ctggtgctga
     1561 ccaacctgac ggcggaagcc ttccgcgaaa acaccgacgc gcggatcagg gacaggctgc
     1621 gggacggcgg cggcaagctg attccgttcg actgggagag ctacc
//
LOCUS       DAXDXW010000141         1630 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXDXW010000141 DAXDXW010000000
VERSION     DAXDXW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.142"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..749)
                     /locus_tag="ACFB64_002111"
     CDS             complement(<1..749)
                     /locus_tag="ACFB64_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC1210378.1"
                     /translation="MNSFIKSTPIVLILILCSSNSYSEPVRLEKSQIKFQSSNNLKSI
                     GFKLDSSSNTFSKFTEAANFEHIPTGAKARINAKITASVSRAGVLSGVGKLVRQGAKF
                     GTRAVPYVGTALLAHDVYETFKEDIKARGCRYDPETDKFVKGYEYANCLWYEDERRIN
                     RTYGCYGVDSSIMRLMPDRSRFPEVKQLMESQMERIARPFWDKRKEEIPKDTALKWRL
                     FPFSHCEFFWNGGGCFVRNGDNLIHRVYFKL"
     gene            complement(691..1008)
                     /locus_tag="ACFB64_002112"
     CDS             complement(691..1008)
                     /locus_tag="ACFB64_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC1210379.1"
                     /translation="MNKPFITQAQLALYKYQPSSEYFGQSMAFIAQKEFEEFVNNVKE
                     YDILESFSYFLNKRVAHNIWKIYFSDESVIFIRKSEENGKTVHEFVYQEYTDSSDFNS
                     MFE"
     gene            complement(1136..1414)
                     /locus_tag="ACFB64_002113"
     CDS             complement(1136..1414)
                     /locus_tag="ACFB64_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210380.1"
                     /translation="MYCQVGNKCLEKHRAENLYFSLVVPRIQENGQIIRPEYNGSMWK
                     MSDGQPLRLSLAECSPKDNLQSGLETGRIVFGVLASVYFVSLLKKVLK"
     gene            complement(1421..>1630)
                     /locus_tag="ACFB64_002114"
     CDS             complement(1421..>1630)
                     /locus_tag="ACFB64_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFC1210381.1"
                     /translation="INTCRKYGAKLAVVTAAPLALAAQANAALPETAKNALEAAKADG
                     MEAGWIVVGVFAALFVFSIVKRVMK"
BASE COUNT          444 a          379 c          325 g          482 t
ORIGIN      
        1 tcaagtttaa aataaactcg atgaattaaa ttatcaccgt tacgtacaaa acaaccgccg
       61 ccgttccaaa aaaattcaca atgactaaaa ggaaaaagac gccactttaa agcagtatct
      121 ttaggaattt cttctttccg tttatcccaa aaaggacgag caatcctttc catttgactt
      181 tccatcagtt gtttgacttc ggggaatctg ctgcgatcgg gcataaggcg cataatcgaa
      241 ctgtcaacgc cgtagcagcc ataggttcta ttaatacgtc tttcgtcttc gtaccaaagg
      301 caattagcat attcgtagcc ttttacaaat ttgtcggttt cgggatcgta tcggcagcct
      361 cgtgccttta tgtcttcttt gaaagtttcg tatacgtcgt gggctaaaag ggctgttccg
      421 acatagggaa ccgcccttgt gccgaatttc gcgccttggc ggacaagttt gccgaccccc
      481 gacaatacgc cggcgcggga tacgctggcg gttattttgg cgttgattcg ggcttttgcg
      541 cccgtgggga tgtgctcgaa atttgccgct tctgtgaatt tggaaaaagt gttagaactt
      601 gaatctaatt taaaaccaat tgattttaaa ttattacttg attgaaattt aatttgtgat
      661 ttttctaatc ttacaggttc agaataagaa ttactcgaac atagaattaa aatcagaact
      721 atcggtgtac tcttgataaa cgaattcatg aacagttttt ccgttctctt ctgattttct
      781 tataaaaata acagactcat cagaaaaata aatcttccaa atattatgtg cgaccctctt
      841 atttaagaaa taagagaagc tctctaaaat atcgtactct tttacgttat taacaaattc
      901 ttcaaattct ttctgagcaa taaaagccat cgattgccca aaatactcgc ttgacggctg
      961 atatttataa agtgccaact gcgcctgcgt gataaacggc ttgttcatgg ttctgccttt
     1021 caaaggttgt tttgaaagcc tgattttgac accataactt catgcgctca atccttaaac
     1081 agaaccgccc cgattaatac ggggacggca acgccgagat agaaataaaa atccatcatt
     1141 tcaaaacctt tttcagcagg gaaacaaagt aaacggacgc gaggacgccg aatactatcc
     1201 ggcctgtttc aagaccgctt tgcaggttgt ctttcggact gcattccgcc aatgaaagcc
     1261 ttagcggctg accgtccgac atcttccaca tgctgccgtt atattccggc ctgattatct
     1321 gtccgttttc ttggattctt ggtactacca agctgaaata aaggttttcg gcccggtgct
     1381 tctcaagaca tttatttccg acttggcagt acatgccgcc ttacttcatc accctcttaa
     1441 cgatggaaaa tacaaaaagc gcggcgaaaa cgcccactac aatccaaccg gcttccatac
     1501 cgtccgcttt tgcggcttcc aaagcgtttt ttgccgtttc gggcaacgct gcgtttgcct
     1561 gtgccgccaa agccagcggg gcggctgtta caacagccag ttttgcgccg tatttacggc
     1621 aggtgttaat
//
LOCUS       DAXDXW010000142         1629 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXDXW010000142 DAXDXW010000000
VERSION     DAXDXW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.143"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(46..1014)
                     /gene="ispH"
                     /locus_tag="ACFB64_002115"
                     /gene_synonym="lytB"
     CDS             complement(46..1014)
                     /gene="ispH"
                     /locus_tag="ACFB64_002115"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC1210382.1"
                     /translation="MNGKTIILANPRGFCAGVDRAISIVERALEEFGAPVYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQNCEIIMIGHKGHAEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNASYLQRTWFEGKSKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGGGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1044..1538)
                     /gene="lspA"
                     /locus_tag="ACFB64_002116"
     CDS             complement(1044..1538)
                     /gene="lspA"
                     /locus_tag="ACFB64_002116"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC1210383.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFAALGKIGAAM
                     IIGGASGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     SKKT"
BASE COUNT          354 a          495 c          409 g          371 t
ORIGIN      
        1 gcatttgtgt tgcccaaaca tccgatgcct gcgttaacgc ctgcgtcaac gtttgcacaa
       61 atcgggtttg gtttcgccct cgcggcgcag ctccttgggc aggacgaaca cgatgctttc
      121 ttccgcgccc cccccttcgc gcacggtttc atgcccccat ccgcgtatgg ttgccaatac
      181 ttcccgcacc aacacttcgg gcgcggacgc gcctgccgtt acgccgactt tgcttttgcc
      241 ttcaaaccac gtgcgttgca ggtaggacgc gttgtccacc atatacgcat cgattccgcg
      301 cgatgccgcc acttcgcgca ggcggttgct gttggacgaa ttgggcgaac cgaccacaat
      361 cacgatgtcg cactgttccg ccagctcttt gacggcggtt tgccggttgg tcgtcgcata
      421 gcagatgtct tccttgtgcg gattgcggat attggggaaa cgcgcgttca gcgcggcgat
      481 gatgtctttg gtttcatcga ccgagagcgt ggtttggctg acataggcga gtttgtcggg
      541 gtttctgact tcgagttttg ccacatctcc gaccgtttcg accaaaagca ttttgcccgg
      601 tgcaagctgc cccatcgtgc cttcgacctc ggcgtgcccc ttatgcccga tcatgatgat
      661 ttcacagttt tgggcatcca gtcgggcgac ttccttatgc actttcgtca ccagcgggca
      721 agtcgcatca aatacccgga aaccgcgctc cgccgcttcc tgctgcaccg ccttcgatac
      781 gccgtgtgcc gaataaacca gtgtcgcgcc cggcggcact tccgccaagt cttcgataaa
      841 caccgcgcct ttttcgcgca ggttgtccac gacgaatttg ttgtggacga cttcgtggcg
      901 cacataaacc ggcgcgccga attcttccaa agcacgttcg acaatactga ttgcccgatc
      961 cacaccggcg cagaagccgc gcggattggc aaggatgatg gtttttccgt tcataagttt
     1021 tgcattccgt gttcagacgg cattcacgtt tttttgctat ctttgcgatg gacgatattg
     1081 tcaagcaccg ccaacaccgc accgacgcag ataaagctgt cggcaatatt aaaggcggga
     1141 taaaaccaat tttgccaata aaacaataag aaatcgacga catgaccgtg tatcaggcga
     1201 tcgatgacat tgcccgacgc accgccgata atcattgccg ccccgatttt gccgagggct
     1261 gcaaactcgt cgcgcaagat ggcgcgtacc aaatacgcgc tcaccgccac cgccagcacc
     1321 aaaaaaaagt atttctgcca gccgccctga tcggcaagga agctgaatgc cgcgcccggg
     1381 ttgtacacca gcgtcagatc gaaaaacgaa gggatgacgt tgacgcgttc ccgatactga
     1441 aacgacgaca gcaccgccca cttcgaccac tggtccagca cgatggcggc aagtgccaat
     1501 acccaatagc gcgttttact tgaaacagat gaagacatat ttttcaacag ccggataaaa
     1561 gagtgccatt ctacccgaaa accccctttc ctgtacccga aacggcaaat gccgtaatct
     1621 tgaaacccg
//
LOCUS       DAXDXW010000143         1477 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXDXW010000143 DAXDXW010000000
VERSION     DAXDXW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.144"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..159)
                     /locus_tag="ACFB64_002117"
     CDS             complement(<1..159)
                     /locus_tag="ACFB64_002117"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HFC1210384.1"
                     /translation="MALGTSANASNFGISLGKSSAASGTKGIAVGTSSQATNLSAVAI
                     GTESKAQNK"
     gene            446..790
                     /locus_tag="ACFB64_002118"
     CDS             446..790
                     /locus_tag="ACFB64_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFC1210385.1"
                     /translation="MNLPFRAMVSDLGGTLLTPEHLVGDLTIDTLRVLEQKGVDIILA
                     TGRNHTDMSSILGKIGAERAVMITSNGARVRDLQGNLLYSNSLPEELVLELYKTSYVG
                     NLIQIPRFCRHQ"
     gene            744..1397
                     /locus_tag="ACFB64_002119"
                     /pseudo
     CDS             744..1397
                     /locus_tag="ACFB64_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
BASE COUNT          450 a          305 c          303 g          419 t
ORIGIN      
        1 tttattttgt gccttacttt cagtaccaat tgccaccgca cttaaatttg ttgcctgaga
       61 agatgtcccc actgcaattc ctttcgtacc actggcagca ctgcttttcc ctaaggaaat
      121 accaaaattc gatgcattag cagaagtacc caatgccact caattttaga tgccgatgct
      181 ttatcgttcc atgataagga gccattatta ccttcttgca aagtagaatc cgccccataa
      241 gaagacaaag cactcattaa caaaatacca gccgctatcg cggacgacct atatacataa
      301 cattacttca tatcatttca ttcagtatca atagtatagg ataactcccc ccccccgtca
      361 atcgaaataa ttcccgtatt ttaatgtcgt attgttcctg tatatttagt gatgtgataa
      421 gtatagttta ttttttgagg tttttatgaa tttacctttt cgggcaatgg tgtctgattt
      481 aggtggcaca cttttaacac ctgaacattt agtgggtgat cttaccattg atactctgag
      541 agtattagag caaaaaggcg tagatattat tttggcaaca ggacgcaatc ataccgatat
      601 gtcatccatt cttggaaaaa tcggtgcgga acgtgcggtt atgattacat caaacggtgc
      661 tcgtgtacgg gatttacagg gtaatttgct ttatagcaat agtttgcctg aagaattagt
      721 tctcgagctt tacaaaacat catatgtggg aaatcttatt cagataccaa gattttgtcg
      781 ccatcaataa acctcaaggc atatccgtcc accgttccgg cggcgaagtc agtctgacgg
      841 caacacttgc ggcacagttg ggcgtagaaa aggtatggtt gctgcacagg ttggacaagc
      901 aggccggagg catattgctg tttgccctta atccccaaag tgccgctgtt cttgccgcac
      961 agtttgccga aaggaaaatg aagaaaacct atttggcttt gtccgaccgc aaaccgtcca
     1021 aaaaacaagg atggataaaa ggcggcatgg aaaaatccag gcgcgggatg tggaagctga
     1081 cgcgcaatat ggaaaatatt gccgttaccc ggtttttcag tatccgcatc agtgaaaaaa
     1141 tgcggctgtt catccttgag ccacatacgg gtaagacaca tcagttgagg gtggtaatga
     1201 agagtctggg cagctctata tttggggaca gcctgtatgg aggaacagaa tccgaaacta
     1261 tgttcctgta tgcgtgaaaa atacaattta cctatcaaca acaagaaatc gaaattaccg
     1321 cacctttgaa aaatgcatgg caggccgaaa atatctctca tgcactggaa gaattttata
     1381 tggcaaaagc aggttaatag atataaatca attaaaacaa tatgataaat atttttataa
     1441 aattttttca aaaaacggtt gaccgcaaat ctcttta
//
LOCUS       DAXDXW010000144         1363 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXDXW010000144 DAXDXW010000000
VERSION     DAXDXW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.145"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..437
                     /gene="mafB"
                     /locus_tag="ACFB64_002120"
     CDS             <1..437
                     /gene="mafB"
                     /locus_tag="ACFB64_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC1210386.1"
                     /translation="KWDWVKNTGYEKPAARPMQTVDGEMAGGNKPIKSLPNSAAEKRK
                     QSFKKFSSNWSSASFDSVHKTLTPNAPGILSPDKVKTRYTSLDGKITIIKDNENNYFR
                     IHDNSRKQYLDSNGNVVKTGNLQGKQAKDYLQQQTHIRNLDK"
     gene            434..790
                     /locus_tag="ACFB64_002121"
     CDS             434..790
                     /locus_tag="ACFB64_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HFC1210387.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMVDWAYENIQSETVVEITENQI
                     IEYQNRGLWGLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            964..1338
                     /locus_tag="ACFB64_002122"
     CDS             964..1338
                     /locus_tag="ACFB64_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC1210388.1"
                     /translation="MIIQNEFNLYPSNMLPEGFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFAGNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
BASE COUNT          507 a          188 c          253 g          415 t
ORIGIN      
        1 ccaaatggga ttgggttaaa aataccggct atgaaaaacc tgctgcccga cctatgcaga
       61 ctgttgacgg ggaaatggca ggtgggaata aacctattaa atctttacca aacagcgccg
      121 ctgaaaaaag aaaacaaagt tttaagaagt ttagtagcaa ctggagttca gcaagttttg
      181 attcagtgca caaaacacta actcccaatg cacctggtat tttaagtcct gataaagtta
      241 aaactcgata cactagttta gatggaaaaa ttacaattat aaaagataac gaaaacaact
      301 attttagaat ccatgataat tcacgaaaac agtatcttga ttcaaatggt aatgttgtga
      361 aaaccggtaa tttacaaggt aagcaagcaa aagattattt acaacaacaa actcatatca
      421 ggaacttaga caaatgaatg aacacaacct gttaattttc tgtttaaaag acaatgtttc
      481 aattagtgaa tatactgaaa tggttgattg ggcttatgaa aacattcaat ctgaaacagt
      541 tgtagaaatt acggaaaatc aaattattga atatcaaaat cgtggattat gggggcttgt
      601 ttctgaaatt accgataatt ggttatttgg accaagtgag ggggattggc taatagataa
      661 ggaaagtatt ttggctgtaa aagaaaaatt acaaaattca gatttttcta cagagccctt
      721 agtgaaaaat attattcatg tacttgaata tgctataaaa aatgaaaaaa cagtaatttt
      781 tcatttttga aactaatcta atttttagta atattgatgc agagcaagca gcattagatg
      841 ccgcaaacat ggggagaagc tattcaattt agaattaaaa aaacaaagtg aaaatggact
      901 agcaccacca aattggtcta cccagtttcc taatggtagt atttatgata ctaaggtaac
      961 gaaatgatta ttcaaaatga atttaattta tatcctagta atatgcttcc tgaagggttt
     1021 tgttatcctg aaaagtatgt tcgtatctct aacgatacat ctttaatacc ttatattcag
     1081 ccacataatt ttcactggtg gtttgagaat tatggaacag aaggggcaga agtagcttat
     1141 atatttagaa attccatcct gcctgattta aatcttatcc cattcgctgg taatggagaa
     1201 tgggaagctt attttgatgg taatgatgta acaggaaatc ctagggttat tgttattaat
     1261 ttagataata tagaaaacca tgaatttttt aatagttttg aagagtggct tgaattagca
     1321 attaaggata cttggtaagc agctatctat aaagagatga ggc
//
LOCUS       DAXDXW010000145         1363 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXDXW010000145 DAXDXW010000000
VERSION     DAXDXW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.146"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(132..1055)
                     /gene="lpxC"
                     /locus_tag="ACFB64_002123"
     CDS             complement(132..1055)
                     /gene="lpxC"
                     /locus_tag="ACFB64_002123"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC1210389.1"
                     /translation="MLQRTLAKSIGVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLNPYLINDTRLSSTIVTDKGLRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSPPTFEIDFAGKSYIGEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IGDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAVNNALLRAV
                     LADETAYEWVEFADSDDLPDAFHELNIRNCG"
BASE COUNT          324 a          330 c          376 g          333 t
ORIGIN      
        1 ggcgggaatc cagccccgtc ggtacggaaa cttatcgggt aaaacggttt cttcagattt
       61 tacgtcctag attcccgccc gcgcgggaat gacggcatct gtttgacggc atttgtttgg
      121 aaaagggggc gttatccgca atttctgatg ttcagctcgt gaaatgcgtc gggcaaatca
      181 tcgctgtcgg caaattccac ccattcgtaa gccgtttcgt ctgccaaaac tgcgcgcaaa
      241 agggcgttgt tgacggcgtg tcccgatttg tagccttcaa acgcgccgac aatcggatgt
      301 ccgacgatat acaaatcgcc gatggcgtcg aggattttgt ggcgcacaaa ctcatcggga
      361 tagcgcaagc cttcaggatt caggacatcc gtgtcgccaa tcacgatggc gttgttcaaa
      421 ttgccgccta agccgagatt gtgggcgcgc atcatttcca cttcgtgcat aaagccgaaa
      481 gtgcgcgcgc gcgcgatttc gccgatgtag gatttgccgg cgaaatcaat ttcaaacgta
      541 ggcgggctgc ggttgaaaac aggatggtcg aattcgatgg tcagcgttac cttaaaaccg
      601 tcatacggcg taaatcggac ccatttgccc gcttctttga tttcgacggg tttgaggatt
      661 ttcaaaaaac gcttttgcgc cttttggtct acaacgcccg catcttgcag gaggtaaata
      721 aacggcaggc tggagccgtc cataatcggg atttcgggcg cgttcaactc aatcagcgca
      781 ttgtcgatgc cgtaggcgga cagtgcggac atgatgtgtt cgatggtgcc gacgcgcagg
      841 cctttgtcgg taacgatggt ggatgaaagg cgggtatcgt tgatcaaata agggttcagc
      901 ttgatttgtt cgcccatctc gccgtccaaa tcggtgcggc ggaaggaaat cccgctgttt
      961 tcaggcgcag ggtgcagggt cagcgcgacg cgttcgcccg aatgcagccc gactccggta
     1021 acgccgatgg atttcgccaa agttctttgc agcataaacc gcttccttat caatggggta
     1081 agtttcggaa taatacgata aaaccggaaa aacaggctat gtttttcaat agtatttgcc
     1141 aatgtatccg tttccaacac gtaagccgca tgaaaatgta tggcggatta acgaaaatca
     1201 ggacaaggcg gcgggccgca ggcagtacgg atagtacgaa aaggcgaggc aacgccgtac
     1261 tggtttaaat ttaattcact atacatcccg tcattcccgc gcgggcggga atctagaatc
     1321 ccggactttc agataatctt tgaatattgc tgttgttcta agg
//
LOCUS       DAXDXW010000146         1265 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXDXW010000146 DAXDXW010000000
VERSION     DAXDXW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.147"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..765
                     /locus_tag="ACFB64_002124"
     CDS             <1..765
                     /locus_tag="ACFB64_002124"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC1210390.1"
                     /translation="RTDADDIAAPGWIEKIVGEMEKDRSIIAMGAWLEILSEEKDGNR
                     LARHHKHGEIWKKPTRHEDIAAVFPFGNPIHNNTMIMRRSVIDGGLRYDTERDWAEDY
                     QFWYDVSKLGRLAYYPEALVKYRLHANQVSSKHSVRQHEIAQGIQKTARNDFLQSMGF
                     KTRFDSLEYRQTKAAAYELPEKDLPEEDFERARRFLYRCFKRTDTPPSGAWLDFAADG
                     RMRRLFTLRQYFGILYRLIKNRRQARSDSAGKEQGI"
     gene            765..>1265
                     /locus_tag="ACFB64_002125"
     CDS             765..>1265
                     /locus_tag="ACFB64_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC1210391.1"
                     /translation="MQNHVISLASAAERRAHIADTFGSRGIPFQFFDALMPSERLERA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGVPYVAVFEDDVLFGKDAEKFLA
                     EDTWLEERFDKDSAFVVRLETMFMHVLTSPSGVADYGGRAFPLLESEHCGTAGYIISR
                     KAMRFFL"
BASE COUNT          299 a          330 c          365 g          271 t
ORIGIN      
        1 cgcaccgatg ccgacgatat tgccgccccc ggctggattg agaaaatcgt gggcgagatg
       61 gaaaaagacc gcagcatcat cgcgatgggc gcgtggctgg aaattttgtc ggaagaaaag
      121 gacggcaacc ggctggcgcg gcaccacaaa cacggcgaaa tttggaaaaa gccgacccgg
      181 cacgaagaca tcgccgccgt tttccctttc ggcaacccca tacacaacaa cacgatgatt
      241 atgcggcgca gcgtcattga cggcggtttg cgttacgaca ccgagcggga ttgggcggaa
      301 gattaccaat tttggtacga tgtcagcaaa ttgggcaggc tggcttatta tcccgaagcc
      361 ttggtcaaat accgccttca cgccaatcag gtttcatcca aacacagcgt ccgccaacac
      421 gaaatcgcgc aaggcatcca aaaaaccgcc agaaacgatt ttttgcagtc tatgggtttt
      481 aaaacccggt tcgacagcct agaataccgc caaacaaaag cagcggcgta cgaattgccg
      541 gagaaggatt tgccggaaga agattttgaa cgcgcccgcc ggtttttgta ccgatgcttc
      601 aaacggacgg acacgccgcc ctccggcgcg tggctggatt tcgcggcaga cggcaggatg
      661 aggcggctgt ttaccttgag gcaatacttc ggcattttgt accggctgat taaaaaccgc
      721 cggcaggcgc ggtcggattc ggcagggaaa gaacagggga tttaatgcaa aaccacgtta
      781 tcagcttggc ttccgccgca gaacgcaggg cgcacattgc cgataccttc ggcagtcgcg
      841 gcatcccgtt tcagtttttc gacgcactga tgccgtctga aaggctggaa cgggcgatgg
      901 cggaactcgt ccccggcttg tcggcgcacc cctatttgag cggagtggaa aaagcctgct
      961 ttatgagcca cgccgtattg tggaaacagg cattggacga aggcgtaccg tatgttgccg
     1021 tatttgagga tgatgtcctg tttggcaaag acgcagaaaa gttccttgcc gaagatactt
     1081 ggttggaaga gcgttttgat aaggattccg cctttgtcgt ccgcttggaa acgatgttta
     1141 tgcacgtcct gacctcgccc tccggcgtgg cggactacgg cgggcgcgcc tttccgcttt
     1201 tggaaagcga acattgtggg acggctggct atattatttc ccgaaaggcg atgcgttttt
     1261 tcttg
//
LOCUS       DAXDXW010000147         1197 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXDXW010000147 DAXDXW010000000
VERSION     DAXDXW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.148"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..654
                     /locus_tag="ACFB64_002126"
     CDS             <1..654
                     /locus_tag="ACFB64_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC1210392.1"
                     /translation="KTGYKPVTARYGNPYQETVYIKVSDGIGNSQTLISNRIHPFYSD
                     GKWIKAEDLKAGNRLFAESGKTQTVRNIVVKPKPLKAYNLTVADWHTYFVKGDKAETE
                     GVWVHNDCPYGGSNNLEKAKLRAERLSKNDRAGKDFTKAGKEAVIDLNRIQNNGQVKC
                     ANCGIETIPAKQSIKNISPTSNERQVDHVIPKSKGGQGTPKNGQVLCRGCNIKKSNK"
     gene            651..1070
                     /locus_tag="ACFB64_002127"
     CDS             651..1070
                     /locus_tag="ACFB64_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HFC1210393.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
BASE COUNT          418 a          225 c          245 g          309 t
ORIGIN      
        1 aaaacaggat acaaacccgt taccgcccga tacggcaatc cgtatcaaga aaccgtttac
       61 attaaagttt cagacggcat cggcaacagc caaaccctga tttccaaccg catccacccg
      121 ttctattcgg acggcaaatg gattaaggcg gaagatttaa aagcgggaaa caggctgttt
      181 gccgaaagcg gcaaaaccca aaccgtccgc aacatcgttg tcaaaccaaa accgctcaaa
      241 gcctacaatc tgaccgttgc cgactggcat acctactttg tcaaaggcga taaggcggaa
      301 acggaagggg tttgggttca taatgattgt ccgtatggcg gttcaaataa tttagagaaa
      361 gcaaaacttc gcgcagaacg gcttagtaaa aacgatcggg caggaaaaga ctttactaaa
      421 gcaggaaaag aagccgttat tgatttgaat aggattcaaa ataatggtca agttaagtgc
      481 gctaattgcg gtattgaaac aattccagcc aaacaatcta taaaaaatat ttctcctact
      541 tcaaatgaaa gacaagtaga tcatgtcatc cctaaatcta agggtggtca aggtacacct
      601 aaaaacgggc aggtattatg cagaggctgc aacattaaga agagtaacaa atgaaatcga
      661 aacttactgt cgtctattac gatttggaaa gcaatattgc agaagaaata ctgtcaggaa
      721 acataatgcc tgatggaaat tttcttattc aagagattcc tcttttcgca ccgaatttgg
      781 ctttaaacga cattgttgcc atagaacgtg aggataagat gctgtttttc gaccacttga
      841 taaaagcttc aggaaatacc acgataaaca tcgttgtttt ggatcatttc ccaaaggatt
      901 tattggcagc catagaagaa cacagtggta aaatcagaaa aaatggagag aattatttat
      961 cggttaattt tccgcccaaa aaatataatt ctgatttaaa aggaatttta aatagatatg
     1021 aggaagcaaa tatcctcagc tacagggaag cttgtttggg cttctcttaa tcgatctata
     1081 agtaagaatg gattacgtta gattgtaaac ctaacgatta aaaggtcgtc tgaaaccgtg
     1141 tttaattttc agacgaccta tttccttcat ttgaaacagg atattgggaa ttaagtt
//
LOCUS       DAXDXW010000148         1165 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXDXW010000148 DAXDXW010000000
VERSION     DAXDXW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.149"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..385
                     /locus_tag="ACFB64_002128"
     CDS             <1..385
                     /locus_tag="ACFB64_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC1210394.1"
                     /translation="KSITSEGKANAATYPKLVNQLNEQNLNNIAAQDPRLSLAIHEGK
                     KNFPIGTATYEEADRLGKIWVGEGARQTSGGGWLSRDGTRQYRPPTEKKSQFATTGIQ
                     ANFETYTIDSNEKRNKIKNGHLNIR"
     gene            390..749
                     /locus_tag="ACFB64_002129"
     CDS             390..749
                     /locus_tag="ACFB64_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC1210395.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            826..1035
                     /locus_tag="ACFB64_002130"
     CDS             826..1035
                     /locus_tag="ACFB64_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210396.1"
                     /translation="MKLIDFEGNLVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKNKVLLLDKELKKIYDTWDKC"
BASE COUNT          444 a          142 c          223 g          356 t
ORIGIN      
        1 taaatctata acgtcggaag gaaaagctaa tgctgcaacc tatcctaagt tggttaatca
       61 gctaaatgag caaaacttaa ataacattgc ggctcaagat ccaagattga gtctagctat
      121 tcatgagggt aaaaaaaatt ttccaatagg aactgcaact tatgaagagg cagatagact
      181 aggtaaaatt tgggttggtg agggtgcaag acaaactagt ggaggcggat ggttaagtag
      241 agatggcact cgacaatatc ggccaccaac agaaaaaaaa tcacaatttg caactacagg
      301 tattcaagca aattttgaaa cttatactat tgattcaaat gaaaaaagaa ataaaattaa
      361 aaatggacat ttaaatatta ggtaattata tgagattaaa attaaaaaat tatgattgtt
      421 ctgactttga tttggaagat ttcccacaag acaagttaga aaatttttgt atacttttga
      481 cattatcaat aggttttgat gaaagtaatg gtgctgatta tttttatgtt tatatatata
      541 gcaccgaatg gttactttca aatatacata gaccgatgtc attaaaaaac tcaattgtta
      601 caaaccgttt taatattgaa cacatattaa aattgataaa tgatatattg gaaatatgta
      661 attcaacgtc tgaagataag tctatatcaa atttggcaaa atattttgac tgggaatttg
      721 acgattataa tctaaatatc caagactaaa gatggtggga cagcatttag accaaaaaca
      781 ggtaaacagt attttgatga caaattaaaa taaaaggaat tattcatgaa attaattgat
      841 tttgaaggga atttagttaa aattagtctc gataaagatg agttgtatat tattcaagca
      901 attgtaggag aaatttattc aggcgtatgc gtggattgta gagattttga gataattcat
      961 ggtgttgaaa aaaataaagt tcttcttttg gataaagaac ttaaaaaaat atatgatact
     1021 tgggataaat gttagctaga tagggtgggc actcgttgcc caccttttgc cgtatattaa
     1081 aaatcctttt caggctgcct gaaactgggt gggcatcaat gccaatccta actgtgcgga
     1141 tgaagcagga aaactgatat gggaa
//
LOCUS       DAXDXW010000149         1126 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXDXW010000149 DAXDXW010000000
VERSION     DAXDXW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.150"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..243
                     /locus_tag="ACFB64_002131"
     CDS             <1..243
                     /locus_tag="ACFB64_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
                     /protein_id="HFC1210397.1"
                     /translation="YFVKGSQAETEGVWVHNDCPYGNLSDNKSVGEGKKFTPAQKKAI
                     IQENMNRNGGVVKSDQSGEVLVRPKKSQKGITPPIK"
     gene            260..349
                     /locus_tag="ACFB64_002132"
     CDS             260..349
                     /locus_tag="ACFB64_002132"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210398.1"
                     /translation="MQAKSKGGSNSYKNAQVLSRRENIKKSNK"
     gene            358..630
                     /locus_tag="ACFB64_002133"
     CDS             358..630
                     /locus_tag="ACFB64_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210399.1"
                     /translation="MQWKFEDPQNTAVITTRRILSKKNGILEVWHDADDGMWQFLDGN
                     EIHEGDAVIVSLKEIVTIDSSVNLLSNLPLGGYAWRNTISEKWNFD"
     gene            797..1027
                     /locus_tag="ACFB64_002134"
     CDS             797..1027
                     /locus_tag="ACFB64_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC1210400.1"
                     /translation="METLDERIKNLGKSLEDRIDANLIDATLEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
BASE COUNT          417 a          172 c          218 g          319 t
ORIGIN      
        1 tacttcgtca agggcagtca ggcggaaacg gaaggggttt gggttcataa tgattgtccg
       61 tatggaaatt tatcggataa taaatctgtt ggcgaaggaa aaaaatttac accagctcaa
      121 aagaaagcaa tcatccaaga aaacatgaat agaaacggag gggtagtgaa gtctgaccaa
      181 tctggagaag ttttggttcg tcctaaaaag agccaaaaag gtatcacccc ccccatcaaa
      241 tgaatggcag attgaccatg tgcaagcaaa aagtaaagga ggttcaaata gttataaaaa
      301 tgcacaggtt ttatccagaa gagaaaatat taagaaatca aataaatagg tatcaatatg
      361 caatggaaat ttgaagatcc tcaaaatact gctgttatta ccaccagaag aattttgtct
      421 aaaaaaaacg gaatcttaga agtatggcat gatgctgatg atggaatgtg gcaattctta
      481 gatggcaatg aaattcatga aggagatgct gtgatagtta gtttaaaaga aattgtcact
      541 attgattcct cagtcaatct cttaagtaat ttacctcttg gtggttacgc ttggagaaat
      601 actatatcgg aaaaatggaa ttttgattag caagcgtagg ttaggttaaa aaaaccaaca
      661 atcaaaggtc gtctgaaacc gtgtttagtt tttcaaacga ccttttcttc aattaacata
      721 gaatgttggg tctcgaccca acctacacgc ttactaatct caaatataac cttatgaaat
      781 aatgagtatt taaaagatgg aaacattaga cgaacgaata aagaatttag gaaaatctct
      841 tgaagataga attgacgcta atctaattga tgccacacta gagtacatta ctttttcaga
      901 acgtttgcta gcttttgaaa cgctatgtga ttatattgaa gatttcaata tccagcttac
      961 ggaaaaagaa tcccaagaaa tctcttttat taataaggaa tttggaattg aaagtacatc
     1021 agattaataa atattaggtc ggtttaaaaa cccaacaatc acaaggtctt ctgaaaccgt
     1081 gtttaatttt cagacggcat ttccttcatt tgaaacagga tattga
//
LOCUS       DAXDXW010000150         1097 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXDXW010000150 DAXDXW010000000
VERSION     DAXDXW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.151"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(246..656)
                     /locus_tag="ACFB64_002135"
     CDS             complement(246..656)
                     /locus_tag="ACFB64_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210401.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIEGTNFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(660..>1097)
                     /locus_tag="ACFB64_002136"
     CDS             complement(660..>1097)
                     /locus_tag="ACFB64_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210402.1"
                     /translation="GNKPAKQATPNGKAGVSTQQSLQNSLVPQKNYTHTTYNGGSAIT
                     DGKYITDPLSMVRHKPDASSLDKIDSKSLFKSDVDAEKATLDAARYADKHNLWDSQNK
                     AKVPTNDVVGYTDGKPTHIINIYRRKPNKDGIRPVHGTPGKER"
BASE COUNT          331 a          193 c          168 g          405 t
ORIGIN      
        1 tgctgaacaa tgttttgtag aacttgctca aaatccatgt ttcgccattt tcctttatat
       61 atacctaatg cagcacgggt tacgatttcg tttaaatcat aaatagccat ttacgtcctc
      121 tgctaatttt cagcgagtag attgggttac caaacccaac aaaaaagcag cctgaaaaat
      181 ttcaggatgc ttgttttatg ttttttatca atgatttagg ttttaatcca gcctgcgctt
      241 gctagttaaa cagtatttac ataaaaatct tgcactgaac aaacaagcgt atcatgttca
      301 tcatgaaatg atgggcataa cgttaatcta caaatgttta catttaaaga tattttttca
      361 gtttcaatat ttttaatttg taaggttaat acatgaggat atttgtgata tagctgattt
      421 tttaattgtt cgttgtctaa tatagcaaaa ggtaattttg cacacaaata accaataact
      481 ttgtttttat gaaaaaaaac tatccaattt tcaattcctt catcccaatc aaaagtaaaa
      541 ttcgttcctt ctatgataaa tttagcacaa gatattaaat ctaacttaac ccaagtggat
      601 ttatacagac tcaatgctga tgaagttata atattatcta aattataaat tttcataaat
      661 caacgttcct tacctggtgt tccatgcaca ggtctaatcc catctttatt cggctttcta
      721 cgataaatgt taattatatg tgtcggtttt ccgtctgtat agccaactac gtcgttcgtt
      781 ggaacttttg ccttattttg actatcccat aaattatgtt tatctgcata tctagcagca
      841 tctaatgtag ctttttccgc atcaacatct gatttaaata aacttttgct atctatcttg
      901 tctagactag atgcatcagg tttgtgtcgt accatactga gagggtcagt aatatatttg
      961 ccatctgtaa tagctgatcc accattatag gtagtatgcg tataattttt ttgtggaacg
     1021 agactatttt gcaaagattg ttgtgtagaa acgccagctt tgccattagg agtagcttgc
     1081 ttcgctggct tattccc
//
LOCUS       DAXDXW010000151         1076 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXDXW010000151 DAXDXW010000000
VERSION     DAXDXW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.152"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..250
                     /gene="exp2"
                     /locus_tag="ACFB64_002137"
     CDS             <1..250
                     /gene="exp2"
                     /locus_tag="ACFB64_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein Exp2"
                     /protein_id="HFC1210403.1"
                     /translation="TLNLFVEGFWIIGLLQALAFTTIVDYCTESKHTNMLNNPACNDE
                     DWDNDMLNNPACNDEDWDNDMLNNPAYSWSMFNIHHSD"
     gene            454..1041
                     /locus_tag="ACFB64_002138"
     CDS             454..1041
                     /locus_tag="ACFB64_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HFC1210404.1"
                     /translation="MIGFVKWYSYEKDFGFIFGEDGHEYFFRKSAGSDIPGQIVNFEP
                     FEHSRGLRARKVSNIHNPDPQQIQKLKTLCKDFQKVEKQPMSKKYNLLSAAILSLCLT
                     MFITLGFFYILAGFFDTDRETNIVLLFVFPAVTFSVLTFFLNRIFRNMKPGSDTDVAT
                     NDDWLKDFWSEDSLIYDPAYSNLNCNIFHRSDDDI"
BASE COUNT          338 a          195 c          216 g          327 t
ORIGIN      
        1 gacattaaat ctttttgttg aggggttttg gataattgga ttacttcaag cactcgcttt
       61 taccaccatt gtagactatt gcactgaatc taaacataca aatatgctca ataatccagc
      121 gtgcaacgat gaagattggg ataatgatat gctcaataat ccagcgtgca acgatgaaga
      181 ttgggataat gatatgctca ataatccagc gtactcttgg tcaatgttca atattcacca
      241 ttcagattaa aaaagcagga gccttatact cctgctttcc gcagcaaagt tacggatgta
      301 caacccggta aaaaaatctc cacttcctat gggcctgcct tggtcttaga aaaaggtgag
      361 aatggcttaa ttaaaattca gccaagcaga ggcaatgaac gttggattac aaatgatagc
      421 attcaagtaa cctataaata aaggagaagt atcatgatcg gattcgtaaa atggtatagc
      481 tatgagaaag attttggctt tatctttggt gaagatggtc atgaatactt tttccgtaaa
      541 tccgccggtt cagatatacc aggacagata gtgaatttcg aaccatttga gcatagtcgt
      601 gggctgcgag cgcgaaaggt tagtaatata cataatcctg atcctcaaca gatccaaaaa
      661 ttgaaaacct tatgcaaaga ctttcagaaa gttgaaaagc aacccatgtc taaaaaatac
      721 aacttgcttt cggcagctat attatcgctg tgtttgacta tgttcataac actgggcttt
      781 ttctacatct tagccggctt ttttgatact gatagggaga ctaacatagt tttgctgttt
      841 gtcttccctg cggtaacttt ttccgtgctg acattctttc tgaataggat attcaggaat
      901 atgaaaccgg ggtctgacac tgatgttgcc actaatgatg attggttaaa ggatttctgg
      961 tctgaggatt cattaattta tgacccagct tattccaatc tcaattgcaa tatatttcat
     1021 cgatcagatg acgatatata agaaagagga ttggtagctg aaatgctgcc aatcat
//
LOCUS       DAXDXW010000152         1053 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXDXW010000152 DAXDXW010000000
VERSION     DAXDXW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.153"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>1053
                     /gene="tuf"
                     /locus_tag="ACFB64_002139"
     CDS             <1..>1053
                     /gene="tuf"
                     /locus_tag="ACFB64_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC1210405.1"
                     /translation="KKFGGAAKAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVD
                     CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCD
                     MVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALKALEGDAAYEEKIFELATALD
                     SYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTT
                     CTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLS
                     KEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEKGVEMVMPGENVTITVELIAPIAMEE
                     GLRFAIREGGRTVGAGV"
BASE COUNT          276 a          296 c          275 g          206 t
ORIGIN      
        1 aaaaaattcg gcggcgctgc aaaagcttac gaccaaatcg acaacgcacc cgaagaaaaa
       61 gcacgcggta ttaccattaa cacctcgcac gtagaatacg aaaccgaaac ccgccactac
      121 gcacacgtag actgtccggg tcacgccgac tacgttaaaa acatgattac cggcgccgca
      181 caaatggacg gtgcaatcct ggtatgttct gctgccgacg gccctatgcc gcaaacccgc
      241 gaacacatcc tgctggcccg tcaagtaggc gtaccttaca tcatcgtgtt catgaacaaa
      301 tgcgacatgg tcgacgatgc cgagctgttg gaactggttg aaatggaaat ccgcgacctg
      361 ctgtccagct acgacttccc cggcgacgac tgcccgatcg tacaaggttc cgcactgaaa
      421 gccttggaag gcgatgccgc ttacgaagaa aaaatcttcg aactggctac cgcattggac
      481 agctacatcc cgactcccga gcgtgccgtg gacaaaccat tcctgctgcc tatcgaagac
      541 gtgttctcca tttccggccg cggtaccgta gtcaccggcc gtgtagagcg aggtatcatc
      601 cacgttggtg acgagattga aatcgtcggt ctgaaagaaa cccaaaaaac cacctgtacc
      661 ggcgttgaaa tgttccgcaa actgctggac gaaggtcagg cgggcgacaa cgtaggcgta
      721 ttgctgcgcg gtaccaaacg tgaagacgta gaacgcggtc aggtattggc caaaccgggt
      781 actatcactc ctcacaccaa gttcaaagca gaagtgtacg tattgagcaa agaagagggc
      841 ggccgccata ccccgttttt cgccaactac cgtccccaat tctacttccg taccactgac
      901 gtaaccggcg cggttacttt ggaaaaaggt gtggaaatgg taatgccggg tgagaacgta
      961 accattactg tagaactgat tgcgcctatc gctatggaag aaggtctgcg ctttgcgatt
     1021 cgcgaaggcg gccgtaccgt gggtgccggc gtg
//
LOCUS       DAXDXW010000153         1042 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXDXW010000153 DAXDXW010000000
VERSION     DAXDXW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.154"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..352)
                     /locus_tag="ACFB64_002140"
     CDS             complement(<1..352)
                     /locus_tag="ACFB64_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210406.1"
                     /translation="MIHKPRYIKIVDENGDFTRVLRLHKFPDTSKVFYFEPMFWLKDG
                     RVARKDSLFEVDYIYGADGCGFLPSNLTEFRKYCRKKHQKFKDDEVLVNRYAVDFLGA
                     KEPPYDDRHVTSVKY"
     gene            complement(473..>1042)
                     /locus_tag="ACFB64_002141"
     CDS             complement(473..>1042)
                     /locus_tag="ACFB64_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210407.1"
                     /translation="WKPPKEAFKYYKSSSRHQKFKKKVPWAVWALIAVVGFVGWKSYG
                     MFQVYSKATDSRIEQEAQKESVVQTMTEQTASSETAPFEHSDNLKPEDFVPTLPEKPE
                     SKPIYNTVRQVKTFEQIAGCIDGGKSDCTCYSNQGTPLKEITKIMCKEYVKNGLPFNP
                     YKDERQRTEQAAQSAKADKPQVLVMGGKS"
BASE COUNT          241 a          253 c          208 g          340 t
ORIGIN      
        1 aatatttgac tgaagtcaca tggcggtcgt catatggggg ttccttcgca cccaaaaaat
       61 cgaccgcgta acggtttact aaaacttcat cgtccttaaa cttttggtgc tttttccggc
      121 aatattttct gaactccgtt aaatttgacg gcaagaaccc gcaaccgtct gcaccgtaaa
      181 tgtaatcaac ttcaaacaaa ctgtctttcc gggcaacccg accatcttta agccagaaca
      241 taggctcgaa ataaaaaact ttcgacgtgt ccgggaactt atggagacgg agaacacgtg
      301 tgaaatcccc gttttcatct acaattttga tatatcttgg tttgtgaatc atgacatcct
      361 cagatttagt attcagaata tgattttaaa aagaactttc tgctttacga ctccgccgcc
      421 gattccttca aacggttttc cgcgctcttc agttgtcgta cattaaattt tattaggact
      481 ttccgcccat tacgagaact tggggcttgt ccgctttcgc ggactgtgcc gcctgttccg
      541 tcctttgccg ttcgtccttg taaggattga aaggcaaccc gtttttcaca tattctttac
      601 acattatctt tgttatttct ttcaagggtg ttccttgatt tgaatagcat gtgcaatctg
      661 attttccgcc gtctatgcat ccggcgattt gctcaaaggt ttttacttgt cggactgtgt
      721 tataaatagg cttgctttcg ggcttttcgg gcaaagtcgg cacaaagtct tcaggtttca
      781 gattgtcgga atgctcaaaa ggcgctgttt ctgatgatgc cgtctgctcc gtcatcgtct
      841 gcacaacgct ttctttttgc gcttcctgct caatccggct gtctgtggct ttgctgtaaa
      901 cttgaaacat gccgtaactt ttccagccta caaaccctac aaccgcaatc aacgcccaaa
      961 ccgcccaagg cacttttttc ttgaactttt ggtgccggct tgatgattta tagtatttga
     1021 aggcttcttt aggcggtttc ca
//
LOCUS       DAXDXW010000154          992 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXDXW010000154 DAXDXW010000000
VERSION     DAXDXW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.155"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(17..247)
                     /locus_tag="ACFB64_002142"
     CDS             complement(17..247)
                     /locus_tag="ACFB64_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210408.1"
                     /translation="MRTHIRTCVYHDSGTKGFKHGIRHKRHGCRRGGTSVFQRGEDGK
                     PQRVSRIRKRSATPGQARERTKTHDVSRTQQP"
     gene            complement(244..>992)
                     /locus_tag="ACFB64_002143"
     CDS             complement(244..>992)
                     /locus_tag="ACFB64_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210409.1"
                     /translation="LQDMRYACNQELINFAEHIVKQVQRLGLYCNTDDPANGESVLFA
                     CREASQAVAQWTKDFDDLSPNQRQLVLRPLSNLFAAYEEFLKDAPARLIAEVSAYSLA
                     VRVAKKAMAFLELDGGLISAVGKVVNGADSRAEARRLKMPYAEFTGRILHAANLLYDV
                     GIQADKELSAMYGRPLNPVRPRRISDVRRPMMKMLVADKGGALVRAVKDSEDVIRHCD
                     NGAGFSCFNWTEHFKRTANLISLMHREAAA"
BASE COUNT          165 a          292 c          295 g          240 t
ORIGIN      
        1 gtagccgccg gcttttttac ggttgttgtg tccttgaaac gtcatgggtt ttcgtccgtt
       61 ctctggcttg tccgggtgtg gcggagcgtt tgcggatgcg gctgacgcgc tgcggtttgc
      121 cgtcttcgcc gcgctgaaaa acgcttgttc cgccgcgccg gcagccgtgc cgcttatgcc
      181 tgatgccgtg tttgaagcct tttgtgccgg aatcgtgata cacgcaggtc cggatgtgcg
      241 tcctcatgcc gccgcttccc tgtgcatgag gctgatcagg tttgccgtcc gtttgaaatg
      301 ttcggtccag ttgaagcagc tgaagccggc gccgttgtcg caatgccgga tgacgtcttc
      361 cgagtctttt acggcccgga ccaacgcgcc gcctttgtcc gcaacaagca ttttcatcat
      421 cggccggcgc acgtcgctta tccgtcgggg gcgcacggga ttcagcggcc tgccgtacat
      481 cgccgaaagc tccttgtccg cctgaatgcc cacatcgtaa agcaggttcg cggcgtgtag
      541 gatccggcct gtaaattccg cgtacggcat tttcaggcgg cgggcttccg cgcgcgaatc
      601 cgcgccgttg acgactttgc cgaccgccga aatcaaaccg ccgtcaagtt ctaaaaacgc
      661 catggctttc ttggcaaccc gcacggccaa tgagtatgcc gatacttcgg ctatcagccg
      721 tgcaggcgcg tctttcagaa attcttcgta cgcggcgaag agatttgaca gcggacgcag
      781 cacgagttgg cgctgattcg ggctaaggtc gtcgaaatcc ttagtccatt gcgcgaccgc
      841 ctgcgatgct tcgcggcagg caaacagtac gctttccccg ttcgcggggt cgtccgtgtt
      901 gcagtacagg cccaagcgtt gcacctgttt gacgatatgt tcggcgaagt tgattaactc
      961 ctgattgcag gcatagcgca tatcctgcag gg
//
LOCUS       DAXDXW010000155          980 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXDXW010000155 DAXDXW010000000
VERSION     DAXDXW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.156"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            53..322
                     /locus_tag="ACFB64_002144"
     CDS             53..322
                     /locus_tag="ACFB64_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210410.1"
                     /translation="MSVKELFKVILDKNKDFPIRTIHRTPMGILPKPVALSIVQYEND
                     PGFYLFYLDETGQEQTDTYHDTLDSAFEQAEFEFGISKEEWMQSP"
     gene            304..456
                     /locus_tag="ACFB64_002145"
     CDS             304..456
                     /locus_tag="ACFB64_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210411.1"
                     /translation="MDAKSLDGKIKMPLYFSDGISVNKKVGIGAQNSLRLAGIGMKSL
                     RKNDYS"
     gene            443..>980
                     /locus_tag="ACFB64_002146"
     CDS             443..>980
                     /locus_tag="ACFB64_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC1210412.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVDNLSDMKRKPF"
BASE COUNT          318 a          150 c          204 g          308 t
ORIGIN      
        1 tggacgtgta atagaaaata gtgtagtaac aattattcag gattagaaaa ttatgagtgt
       61 aaaagaatta ttcaaagtta tcttagataa aaataaagat tttcctatta gaacgattca
      121 tcgcactcca atggggatac ttcccaaacc tgttgcttta agtattgttc aatatgaaaa
      181 tgacccggga ttttatttgt tttatttgga tgagaccggt caggaacaaa cggatactta
      241 tcatgacaca ttagattcgg catttgaaca ggctgaattt gaatttggaa tcagcaaaga
      301 ggaatggatg caaagtcctt agacggcaaa atcaagatgc cgctttattt ttcagatggc
      361 atttccgtca ataaaaaagt cggtattgga gcccaaaaca gcctacgtct tgccgggata
      421 gggatgaaat ctttaagaaa aaatgattat agttgaacat ttagaacatt atttgggtga
      481 aatcgaaagc ggtataaaat gcttggatcg acgttatcat ctcagcgttt ccgtcttccc
      541 atcacaacca tataaagggg taactacttt ttctacttta ggcttgaatc gatatgactt
      601 gaactataag agtaggtttg aattaatttt tacctgttct gaagaatgga ataaagaaaa
      661 tattgcagct tttctatcag gagtggctga atatttgatt gacaatagac aacctatttt
      721 aagaggtgaa attattcaat tacccagggt aattatcgaa ggtagcaaaa tggatgcact
      781 gtatgtttcc gccccatttt atttcgatga tgattttcag gtctgttatg gcgaacacta
      841 caatatcgtt ttccctttgc ttgtcccctt gtataaacag gaagcagaat tggtggaaaa
      901 gaaaggttgg aatgcttttg agcagttctt gctggataat gaagttgaca acctttcgga
      961 tatgaagcgg aaaccgtttg
//
LOCUS       DAXDXW010000156          921 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXDXW010000156 DAXDXW010000000
VERSION     DAXDXW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.157"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..551
                     /gene="mafB"
                     /locus_tag="ACFB64_002147"
     CDS             <1..551
                     /gene="mafB"
                     /locus_tag="ACFB64_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC1210413.1"
                     /translation="KWDWVKNTGYETTVTRKTLDGQIAGKNKPIKPSSGDYVLPVGRQ
                     IIDPTRTSFSQATVSYQKRGANYNYDSLVAAMNEKKSWVGDRVDVVNMPDGAPTSMDN
                     TRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQTWGEAIQLRIRKQETQKG
                     VPEGWSKRFPNGSIYDVKVLRK"
     gene            548..919
                     /locus_tag="ACFB64_002148"
     CDS             548..919
                     /locus_tag="ACFB64_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC1210414.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
BASE COUNT          326 a          146 c          190 g          259 t
ORIGIN      
        1 ccaaatggga ttgggttaaa aataccggct atgaaacaac tgttacccga aagactttag
       61 atgggcaaat agctggcaag aacaagccta ttaaaccttc ttcaggagat tatgtcttgc
      121 cggtaggcag acaaatcata gatcctactc gtacatcttt ttcacaagct acagttagct
      181 atcagaagcg tggtgcaaat tacaactatg attctcttgt agctgcaatg aacgaaaaaa
      241 aatcatgggt tggcgacagg gttgatgttg ttaatatgcc tgatggagca cctactagta
      301 tggataacac gcgtattatg gcagcacgtg aagcaggggt aaaagtggaa gcgaatgttc
      361 ataattttaa tgaccgatta tcatcaaaag agagaatcag gtttaagcat gatggtattg
      421 agcctcaaac ttggggagaa gctatccagc tacgaattag aaagcaagaa acacaaaaag
      481 gagttccaga agggtggagc aaaagatttc ctaacggaag tatttatgat gtaaaggtac
      541 ttaggaaatg ataaaacaaa atagttttgt tccgtatcct gaagcaatgc ttcctaaagg
      601 atttaaatat ccgcaaagtt atttaaaatt agctcaatcc actcatgcca ttaactacga
      661 tgaacaatat tcttttcctt ggtggtttga aaatgcagaa agcaatatat cagaagtaat
      721 tgacatttat tttgaaataa ctggcattcc aaacctatta ccttttgcta gaaaccaaga
      781 gtgggctgcc tgttttgata tttcagataa atcaggtaat cctaaaatta tagtagttaa
      841 tttagataat acaaaatatt acgagacttt tgaaaatttt gatacttggc taaaagaagc
      901 tgaaaatgat ggttggtagc a
//
LOCUS       DAXDXW010000157          920 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXDXW010000157 DAXDXW010000000
VERSION     DAXDXW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.158"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(232..516)
                     /locus_tag="ACFB64_002149"
     CDS             complement(232..516)
                     /locus_tag="ACFB64_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC1210415.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMPVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWRDLWFDII"
BASE COUNT          256 a          235 c          167 g          262 t
ORIGIN      
        1 cggagcggtt tctgtttttt ccgataaatg ccgcaatctc aaatcccgtc attcccacga
       61 aagtgggaat ctaggcctgt cggcacagaa acttatcggg taaaacggtt tctttagatt
      121 ttacgttcta gattcccgcc tgtgcgggaa tgacggttta gaagttgccc gaaacctcaa
      181 aaaaaaccga aaccgaacaa gccggattcc cgcctgagcg ggaatgacgg gctaaataat
      241 atcaaaccat aaatcccgcc aagaaacatt attttcttca atcagttgca atttccaagc
      301 cctgttccat ttcttcaact gtttttcccg agtaattgcg ctctccatcg taggatgcag
      361 ttcataccaa accggcatag taacgttgta ccgtgatgta aatccttcaa tcaaatgctc
      421 cctatgttgg taaatacgtt gcaccaaatc agatgtaacg ccaatgtata acgtgccatt
      481 acgttggctt gctaaaatat aaaccgcagg ctgcatataa tacccttttg aattatttaa
      541 atttatattc ccgcgaacac catccagtga ttactttaac ccttcgttat ccccatagct
      601 ttccatcatt cccgcaactc ttcgtcattc ccgcgaaagc gggaatccag aatctctaaa
      661 gcttcagcta acctttgaat attgctgttg tcccacgttc tagattcccg cctgagcggg
      721 aatgacggcg gagcggtttc tgttttttcc gataaattcc taaaactcaa aatttcatca
      781 ttcctacaaa aacagaaaat caaaaacaga aacctaaaat cccgtcattt ccacgacaat
      841 gggaatccgg ttcgttcggt ttcgcttgtt ttaagtttcg ggtaacttcc acttcgtcat
      901 tcccgcgcag gcgggaatcc
//
LOCUS       DAXDXW010000158          888 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXDXW010000158 DAXDXW010000000
VERSION     DAXDXW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.159"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>888
                     /gene="pilC"
                     /locus_tag="ACFB64_002150"
     CDS             <1..>888
                     /gene="pilC"
                     /locus_tag="ACFB64_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC1210416.1"
                     /translation="VDLGTLRPRVEAKVRRGWSLLNFWATWDIKDTGQIPVKLGLPEV
                     KAGRCINKPNPNPKSALSPALTAPALWFGPVQNGKVQMYSASVSTYPGSSSSRIFLQE
                     LKTKTDPGRPGRHSLAALNAQDIKSREPNFNSRQTVIRLPGGVYRIAPTRDRIVGLNG
                     NDGKNDTFGIYKERLVTPDADEWAKVLLPWTVRYYGNDDIFKTFNQPNSKTQNGKKQY
                     SQKYRIRTKEDDNDKPRDLGDIVNSPIVAVGGYLATSANDGMVHIFKKTGTDERSYNL
                     KLSYIPGTMERKDIEGNDS"
BASE COUNT          242 a          250 c          234 g          162 t
ORIGIN      
        1 agtggatttg ggcaccttga gaccgcgcgt cgaggcaaag gtaaggcggg ggtggagtct
       61 gctaaatttt tgggctacgt gggacattaa agataccggg cagattccgg tcaagctcgg
      121 tctgccggaa gtcaaggccg ggcgctgcat caacaaaccg aaccccaatc ccaaatcagc
      181 cctttcgccg gcactgaccg cccccgcgct gtggttcggt cctgtgcaaa atggcaaggt
      241 gcagatgtat tccgcttcgg tttccaccta ccccggcagc tcgagcagcc gcatcttcct
      301 ccaagagctg aaaactaaaa ccgaccccgg caggcccggc cggcattccc tcgccgcttt
      361 gaatgcgcag gatatcaaat cccgcgagcc gaatttcaac tcaaggcaga ccgtcatccg
      421 attgccgggc ggcgtgtacc ggatcgcccc gactcgcgac aggatcgtgg gtttgaatgg
      481 caatgacggc aaaaacgaca ctttcggcat ctacaaggaa aggttagtca cacctgatgc
      541 cgacgagtgg gcaaaagtgc tgctgccttg gacggtccgg tattacggta atgacgatat
      601 atttaaaaca ttcaaccaac caaacagcaa aacacaaaac ggcaaaaaac aatacagcca
      661 aaaataccgc atccgcacaa aagaagatga caatgacaaa ccccgcgatt tgggcgacat
      721 cgtcaacagc ccgattgtcg cggtcggcgg gtatctggca acttctgcca acgacgggat
      781 ggtgcatatc ttcaaaaaaa ccggcacgga tgaacgcagc tacaatctga agctcagcta
      841 catccccggt acgatggagc gtaaggatat tgaaggcaat gactccga
//
LOCUS       DAXDXW010000159          887 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXDXW010000159 DAXDXW010000000
VERSION     DAXDXW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.160"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..748
                     /locus_tag="ACFB64_002151"
     CDS             <1..748
                     /locus_tag="ACFB64_002151"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFC1210417.1"
                     /translation="RFIDVNPEDFAGFPLNIRHISITTYARLKLGEYIADCDKVLYLD
                     TDVLVRDGLKPLWDTDLGGNWVGACIDLFVERQEGYKQKIGMADGEYYFNAGVLLINL
                     KKWRRHDIFKMSCEWVEQYKDVMQYQDQDILNGLFKGGVCYANSRFNFMPTNYAFMAN
                     GFASRHTDPLYLDRTNTAMPVAVSHYCGSAKPWHRDCTVWGAERFTELAGSLTTVPEE
                     WRGKLAVPPTKRMLQRWRKKLSARFLRKIY"
     gene            808..>887
                     /locus_tag="ACFB64_002152"
     CDS             808..>887
                     /locus_tag="ACFB64_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase 2 family protein"
                     /protein_id="HFC1210418.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLSAVVG"
BASE COUNT          220 a          217 c          245 g          205 t
ORIGIN      
        1 ccgctttata gacgtaaacc ccgaagattt cgccggcttc cccttaaaca tcaggcacat
       61 ttccattacg acttatgccc gcctgaaatt gggcgaatac attgccgatt gcgacaaagt
      121 cctgtatctg gatacggacg tattggtcag ggacggcctg aagcccttat gggataccga
      181 tttgggcggt aactgggtcg gcgcgtgcat cgatttgttt gtcgaaaggc aggaaggata
      241 caaacaaaaa atcggtatgg cggacggaga atattatttc aatgccggcg tattgctgat
      301 caacctgaaa aagtggcggc ggcacgatat tttcaaaatg tcctgcgaat gggtggaaca
      361 atacaaggac gtgatgcaat atcaggatca ggacattttg aacgggctgt ttaaaggcgg
      421 ggtgtgttat gcgaacagcc gtttcaactt tatgccgacc aattatgcct ttatggcgaa
      481 cgggtttgcg tcccgccata ccgacccgct ttacctcgac cgtaccaata cggcgatgcc
      541 cgtcgccgtc agccattatt gcggctcggc aaagccgtgg cacagggact gcaccgtttg
      601 gggtgcggaa cgtttcacag agttggccgg cagcctgacg accgttcccg aagaatggcg
      661 cggcaaactt gccgtcccgc cgacaaagcg tatgcttcaa agatggcgca aaaagctgtc
      721 tgccagattc ttacgcaaga tttattgacg gggcaggccg tctgaagcct tcagacggca
      781 tcggacgtat cggaaaggag aaacggattg cagcctttag tcagcgtatt gatttgcgcc
      841 tacaacgtag aaaaatattt tgcccaatcg ctgtccgccg tagtggg
//
LOCUS       DAXDXW010000160          879 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXDXW010000160 DAXDXW010000000
VERSION     DAXDXW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.161"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..289
                     /locus_tag="ACFB64_002153"
     CDS             <1..289
                     /locus_tag="ACFB64_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFC1210419.1"
                     /translation="KLKAAKARNQSLKDYLARNMQAAGITEIKADDGTFKASFRKSEA
                     VVILDEAQIPAEFMREAVKTEPDKTAIRKAIESGRQVAGAKIEGRKNLQIR"
     gene            341..556
                     /locus_tag="ACFB64_002154"
     CDS             341..556
                     /locus_tag="ACFB64_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210420.1"
                     /translation="MSYLEDVKNALRVIDNLCKEALKEPESLEGYIDEIRDKADEADT
                     SLEFLKDVINYGISDLKNVIEVFEDCV"
     gene            667..>879
                     /locus_tag="ACFB64_002155"
     CDS             667..>879
                     /locus_tag="ACFB64_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210421.1"
                     /translation="MQTVATKPTAKQMLAAKRAAKESTRQERAVKRAGTVKNVDRNRL
                     SARSKAQKENIARMLSGAKVSEDEALT"
BASE COUNT          312 a          175 c          230 g          162 t
ORIGIN      
        1 gaagctcaag gcggcaaaag cgcggaatca aagcctgaaa gactacttgg cgcgcaatat
       61 gcaggcggcg ggcattaccg aaatcaaagc ggacgacggc acttttaaag cctcgttccg
      121 caaatccgaa gccgtcgtga tcttagacga agcacaaatc cccgccgaat ttatgcgtga
      181 ggccgtcaaa accgaaccgg acaaaaccgc catcagaaaa gcgattgaaa gcggtcggca
      241 agtagcaggc gcgaagattg aagggcggaa gaatttgcag attagataaa ccgtagaaga
      301 tgttgacgac agcatcccat tttgagttaa ggagcaaaaa atgagctatt tggaagatgt
      361 aaaaaacgca ttaagggtaa tagataactt atgcaaagaa gcattaaaag agcctgaatc
      421 gttagagggt tatatagacg aaattaggga taaagcagac gaagcggata cctctttgga
      481 atttctaaag gatgtaataa attatggtat tagcgattta aaaaatgtaa ttgaggtgtt
      541 tgaagattgc gtttgatatt aatggaacaa gacatgaact atcaatttaa attcggcgac
      601 cccgtgagag atacagcttt atgattgggc attgagagaa cggcatcaaa aaatcgagaa
      661 aacaaaatgc aaacagtagc aacaaaaccg acggcaaaac agatgcttgc cgccaaacgc
      721 gcggcgaagg aatcaacgcg gcaggaacgc gccgtcaaac gcgcgggaac agtaaaaaac
      781 gttgaccgga accggctgtc cgcccggtca aaagcgcaaa aagaaaacat cgcccggatg
      841 ttgtcgggcg caaaggtatc ggaagacgaa gccctgacg
//
LOCUS       DAXDXW010000161          867 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXDXW010000161 DAXDXW010000000
VERSION     DAXDXW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.162"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>867
                     /gene="pilC"
                     /locus_tag="ACFB64_002156"
     CDS             <1..>867
                     /gene="pilC"
                     /locus_tag="ACFB64_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC1210422.1"
                     /translation="VDLGILKPHVETTGRSLLDFWARWDIKDTGQIPVKLGLPQVKAG
                     RCTNKPNPNNNTKAPSPALTAPALWFGPGQDGKAEMYSASVSTYPGSSSSKIFLQNLS
                     RNDDKNKPGRYSLKPLSENEIKSKEPSFMGRQTIIRLDDGVHLIKLNGSKDEVAAFVN
                     LNGNNTGKNDTFGIVKEANVNLEADEWKKVLLPWTVRGPGNDDKFKSINREPGKYSQR
                     YRIRENGNRDLGDIVNSPIVAVGEYLATAANDGMVHIFKKNGGSDERSYNLKLSYIPG
                     TMPRKDIESKDST"
BASE COUNT          256 a          218 c          223 g          170 t
ORIGIN      
        1 agtggatttg ggtatcttga aaccgcacgt cgagacgaca ggacgaagct tgctagattt
       61 ttgggctagg tgggacatta aagataccgg gcagattccg gtcaagctcg gcctgccgca
      121 agtcaaagca ggccgctgca ccaacaaacc gaaccccaat aataatacca aagccccttc
      181 gccggcactg accgcccccg cgctgtggtt cggacccggg caagatggta aggcggagat
      241 gtattccgct tcggtttcta cctaccccgg cagctcgagc agcaaaattt tcctgcaaaa
      301 cctttcccgc aatgatgaca aaaacaaacc gggccgctat tccctcaaac ccttgagtga
      361 gaatgagatt aaaagtaaag agccgagttt catggggcgg caaacaatca tccgattgga
      421 tgacggcgta catttgatca aactgaatgg aagcaaggat gaggtcgccg cttttgtcaa
      481 tttaaatgga aacaacaccg gcaaaaacga cactttcggc attgttaagg aagcgaacgt
      541 caatcttgaa gccgacgagt ggaaaaaagt gctgctgcct tggacggttc ggggtcccgg
      601 taatgatgat aaatttaaat caattaaccg agaaccaggc aaatacagcc aaagataccg
      661 catccgcgaa aacggcaatc gcgatttggg cgacatcgtc aacagcccga ttgtcgcggt
      721 cggcgagtat ttggcaaccg ccgcgaacga cgggatggtg catatcttca aaaaaaacgg
      781 cggcagtgat gaacgcagct acaatctgaa gctcagctac atccccggta cgatgccgcg
      841 caaggatatt gaaagcaaag attctac
//
LOCUS       DAXDXW010000162          829 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXDXW010000162 DAXDXW010000000
VERSION     DAXDXW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.163"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..285
                     /locus_tag="ACFB64_002157"
     CDS             <1..285
                     /locus_tag="ACFB64_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC1210423.1"
                     /translation="GNKPVVKSIRPTTRDELRQALQEQGFRRTGSDAAQYETWKGPDG
                     VKIDIRPNGEVIRTQRVPRTDGVQGKYPQRQDYEGNPLPNNHHHSGYFVK"
     gene            282..599
                     /locus_tag="ACFB64_002158"
     CDS             282..599
                     /locus_tag="ACFB64_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210424.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            669..829
                     /locus_tag="ACFB64_002159"
                     /pseudo
     CDS             669..829
                     /locus_tag="ACFB64_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
BASE COUNT          291 a          144 c          159 g          235 t
ORIGIN      
        1 ggaaacaagc cagttgttaa atctattaga ccaactacgc gagatgaatt acgtcaagca
       61 ttgcaagaac aaggttttag acgtactggt tcagatgcgg ctcaatatga aacatggaaa
      121 ggtcctgatg gcgtgaaaat agatattcgt ccaaatggag aggttataag aacccaaaga
      181 gtgccgcgaa ccgatggtgt acagggaaaa tatccgcaac gacaagatta tgaaggcaat
      241 ccattgccaa ataatcatca tcattctgga tattttgtca aatgaaaaaa aatatttttc
      301 acaatgtaag cctttatgaa ataatctttt ccgataatgg aaataccctt acattatctt
      361 ttacagatac aattgaaggt aattatttcg gatatatcaa atgcagtaat attttgaatt
      421 ttaaattaga tacaaataat ttcgtagatt atgaggataa ggaagatagc ttgtttccct
      481 tgtttatacc cgaaatagag ctatataaat accaatttta tagtgaaatt attattgatg
      541 tagggattat tataaaaata tctgctgaaa caattaattt tgagccactg ggaaaatagt
      601 aactgctttc ccagcagccg tagcaactgt atttttaccc gacggggtaa aaatacagtt
      661 gctacatctc tgcgcagcag actctacaag gtattaataa ttcaggaaaa ttaagtccgg
      721 aagcacaact tgccgccgtg agcctattac aggacagtgc ttttgcggta aaagacggta
      781 tcaactctgc cagacaatgg gctgatgccc atccgaacat tacagctac
//
LOCUS       DAXDXW010000163          829 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXDXW010000163 DAXDXW010000000
VERSION     DAXDXW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.164"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            27..>829
                     /gene="mafA"
                     /locus_tag="ACFB64_002160"
     CDS             27..>829
                     /gene="mafA"
                     /locus_tag="ACFB64_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC1210425.1"
                     /translation="MRARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKG"
BASE COUNT          235 a          205 c          188 g          201 t
ORIGIN      
        1 ttgtttttca tcaaaggaga aaatctatgc gggcacggct gctgatacct attctttttt
       61 cagtttttat tttatccgcc tgcgggacac tgacaggtat tccatcgcat ggcggaggca
      121 aacgcttcgc ggtcgaacaa gaacttgtgg ccgcttctgc cagagctgcc gttaaagaca
      181 tggatttaca ggcattacac ggacgaaaag ttgcattgta cattgcaact atgggcgacc
      241 aaggttcagg cagtttgaca gggggtcgct actccattga tgcactgatt cgcggcgaat
      301 acataaacag ccctgccgtc cgtaccgatt acacctatcc gcgttacgaa accaccgctg
      361 aaacaacatc aggcggtttg acgggtttaa ccacttcttt atctacactt aatgcccctg
      421 cactctcgcg cacccaatca gacggtagcg gaagtaggag cagtctgggc ttaaatattg
      481 gcgggatggg ggattatcga aatgaaacct tgacgaccaa cccgcgcgac actgcctttc
      541 tttcccactt ggtacagacc gtatttttcc tgcgcggcat agacgttgtt tctcctgcca
      601 atgccgatac agatgtgttt attaacatcg acgtattcgg aacgatacgc aacagaaccg
      661 aaatgcacct atacaatgcc gaaacactga aagcccaaac aaaactggaa tatttcgcag
      721 tagacagaac caataaaaaa ttgctcatca aacccaaaac caatgcgttt gaagctgcct
      781 ataaagaaaa ttacgcattg tggatggggc cgtataaagt aagcaaagg
//
LOCUS       DAXDXW010000164          797 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXDXW010000164 DAXDXW010000000
VERSION     DAXDXW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.165"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            1..79
                     /locus_tag="ACFB64_002161"
                     /pseudo
     CDS             1..79
                     /locus_tag="ACFB64_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            696..>797
                     /locus_tag="ACFB64_002162"
     CDS             696..>797
                     /locus_tag="ACFB64_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFC1210426.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDY"
BASE COUNT          260 a          219 c          140 g          178 t
ORIGIN      
        1 cgaagccatc cttttggccg aaggtcaaaa atcagccgtc accgagtatt gcccgaatca
       61 cggcatatgg ccggaaaact tcgtcattcc cgcgaaagcg ggaatctagg tctgtcggca
      121 cggaaactta tcgggtaaaa aggtttctcc ggtcctgagt cctggattcc cactttcgtg
      181 ggaatgacgg gatttaatga tgccgccggc aacgacaaaa tcgaaaccaa gcacctgccg
      241 tcaacctgcc gcgataaatc atctgccgtt tgcacgaaac accacgcgcc gatttcaaat
      301 gctttccaag aaaacggagc tttttaaaaa ataaaaaatt ccccacccaa cccaccctat
      361 tctaacacgt aaattcaaaa atctcaaatt ccgacccaat caacacaccc gataccccat
      421 gccaataaaa aagtaacgaa aatcggcact aaaactgaca attttcgaca ctgccgcccc
      481 ctacttccgc aaaccacacc cacctaaaag aaaatacaaa ataaaaacaa ttatatagag
      541 ataaacgcat aaaatttcac ctcaaaacat aaaatcggca cgaatcttgc tttataatac
      601 gcagttgtcg caacaaaaaa ccgatggtta aatacattgc atgatgccga tggcgtaagc
      661 ctgaggcatt tcccctttca attaggagta attttatgaa tacccttcaa aaaggcttta
      721 cccttatcga gctgatgatt gtgatcgcca tcgtcggcat tttggcggca gtcgcccttc
      781 ccgcctacca agactac
//
LOCUS       DAXDXW010000165          789 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXDXW010000165 DAXDXW010000000
VERSION     DAXDXW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.166"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(142..543)
                     /locus_tag="ACFB64_002163"
     CDS             complement(142..543)
                     /locus_tag="ACFB64_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210427.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKTENNSDGNPTANYFFEIIDDSYMDWLKEESFGF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
BASE COUNT          220 a          160 c          133 g          276 t
ORIGIN      
        1 agccaacaac ttcggcaaaa tcaagccggc aagcccctgc atcctgtact aatcggagaa
       61 aatgccgtct gaaagccgaa taccgcttca gacggcattc tgattgttgg gtttttaatc
      121 agagctttgt ttgataattt gttattttga ataaaataca ggttctgtcg agctgataac
      181 ttctattaca gaatcgctaa aaaagaaaat ataagcctta taatatttct tttcaaaaaa
      241 accaaaactt tcttctttca accagtccat ataactgtca tctattatct cgaaaaaata
      301 atttgcagta ggattcccat ccgaattatt ttctgtttta ttttcgaata aatattctga
      361 aaattttact ttataagatt catccgttat ttgaaaatga attatattgt caataaatat
      421 ttcaatattt aattcataat ttcggtcgga tgccaaaatg ttcacatctt gttcggtaac
      481 acaaattttg tccacataaa atctgtgatt attgttagaa aattgtaatg gtttaatctt
      541 cacaatcgtt ttccatatct aagttttgtt ttcttattgc cctgccggat aaagcaggtc
      601 tgcctccgta ctgtctatcc tcacatttac tttcctgcca tcggctttta tctccccggt
      661 tttaccgttt ttgccaactt cgcggctttg gcggtggcaa tgttgaaggc ggcttcgacg
      721 gtttcggcgg cattggggtt gacccgtact catccgcaaa cggcaacacg acggacggtg
      781 tttgctttt
//
LOCUS       DAXDXW010000166          776 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXDXW010000166 DAXDXW010000000
VERSION     DAXDXW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.167"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..431
                     /locus_tag="ACFB64_002164"
     CDS             <1..431
                     /locus_tag="ACFB64_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC1210428.1"
                     /translation="ISDSLVRKWVARYRLHGESGIKRRKHTTKYSVEYKLEAIRPVTG
                     QGMSQKAAADQLNLPDCSVLPQWLRLYRLNGINGLKPKPKGRKPVKKQYPPQTKKADY
                     LKTKEELFAELAYLKAEAAVLKKLDALKEVRQKERNSSQG"
     gene            436..>776
                     /locus_tag="ACFB64_002165"
     CDS             436..>776
                     /locus_tag="ACFB64_002165"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFC1210429.1"
                     /translation="MPSVELLLGIVGLPRSTFYYQLAVQSAEGKYADLKRHIHDIYQR
                     YKGRYGYRRIAAAIRHAGIPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKVAPNI
                     LQRCFHSEKPN"
BASE COUNT          241 a          187 c          200 g          148 t
ORIGIN      
        1 ccatttccga ttcattggta cgcaaatggg tggcaagata cagattacac ggtgaaagcg
       61 gcatcaaacg cagaaagcat acgacaaaat attcggtcga atacaaactt gaggcaatcc
      121 gcccggtgac ggggcaggga atgtcccaaa aagctgccgc agaccaactg aatctgcccg
      181 actgctccgt tttgccgcaa tggttgcgcc tctaccgttt gaatggtatt aacggtttaa
      241 agcccaaacc caaaggaaga aagcccgtga aaaaacagta tccgccgcaa acgaaaaaag
      301 ccgactatct gaaaaccaag gaagaactgt ttgcggaatt ggcttacctt aaagcggaag
      361 cggctgtcct aaaaaagctc gatgccttga aagaagtgcg gcagaaagaa cgcaactcgt
      421 cgcagggtta aggaaatgcc atccgttgaa ctgctgttgg ggattgtcgg actgccacgc
      481 agcaccttct attaccaatt ggccgtccaa tcggcagaag gcaaatatgc cgatttgaaa
      541 cgacatatcc atgatattta tcaacgatat aagggaagat acggctaccg gaggattgcg
      601 gcagccatcc gtcacgcagg aataccggtc aatcacaaga aagtcagccg tctgatggcg
      661 aagacggggc tgaaggcagt gatacggcgg cgcaaatacc gctcgttcaa aggagaagtc
      721 ggcaaagttg cgccgaacat cctgcaacgc tgtttccatt cagaaaagcc gaatga
//
LOCUS       DAXDXW010000167          770 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXDXW010000167 DAXDXW010000000
VERSION     DAXDXW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.168"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(99..422)
                     /locus_tag="ACFB64_002166"
     CDS             complement(99..422)
                     /locus_tag="ACFB64_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210430.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEHDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(425..613)
                     /locus_tag="ACFB64_002167"
     CDS             complement(425..613)
                     /locus_tag="ACFB64_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HFC1210431.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNINKVEI
                     HYIENTRTGEKTDFKFKD"
BASE COUNT          197 a          134 c          106 g          333 t
ORIGIN      
        1 tatttttatt tccagtcatt ctggctattt tatgtatatt tccattatgt ttgattatca
       61 ttttattttt tccaaggatt ttcttctata ttgatagttt atctaaagat ttcatttaaa
      121 atatcctgac taatttcttg tttatataat tctacaattt tctgtttctg tgtatttgta
      181 taaaattttt cgttggtatc tgcttgatat tttaattgtc tcaatatttc catgatttca
      241 tattctgatt tttcatcatg ttcttcaatc atgtaatcaa caaattgttt tagaatatca
      301 aaggcttcgt aactttccaa aattttaata tgtttatcgg caaaattgga catataccat
      361 tcgtcagtga ttggattggt atttaaatca tctaaaaaaa catttacatc atctaaaaac
      421 atggctaatc cttaaacttg aaatctgttt tctcaccggt tcttgtattt tcaatgtaat
      481 gtatctcaac tttgtttata ttttgaacgc gcttaaccca gccgtctttt gccaaccaac
      541 catttttagt atcggacata ggaggtattc tcgcaggagt tttgccctgt gggtttgcct
      601 taacttcttc cattgctaat tgttccttta aattattagg tattgtacga ccggttgatt
      661 tttcaacaga ttgtgtccct atttttaatg atttgtttcc ccccagccat ttccctgtct
      721 acagtctgca tatggcgggc agtaggtttt tcatagccgg tatctttaac
//
LOCUS       DAXDXW010000168          735 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXDXW010000168 DAXDXW010000000
VERSION     DAXDXW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.169"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(123..425)
                     /gene="imm40"
                     /locus_tag="ACFB64_002168"
     CDS             complement(123..425)
                     /gene="imm40"
                     /locus_tag="ACFB64_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm40 family immunity protein"
                     /protein_id="HFC1210432.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPTAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSINKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(441..>735)
                     /locus_tag="ACFB64_002169"
     CDS             complement(441..>735)
                     /locus_tag="ACFB64_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210433.1"
                     /translation="ESKNTVTTNNFFENTGYTEKVLRQASNGDYHGFPQSVDAFSENG
                     TVIQIVGGDNIVRHKLYIPGSYKGKDGNFEYIREADGKINHRLFVPNQQLPEK"
BASE COUNT          224 a          133 c           93 g          285 t
ORIGIN      
        1 attaatacct tgtagagtct gctgagcggc tgtatctgat ccggtcagcg taggttaggt
       61 taaaacccag cattttcaac atcttgatgt tttactataa acctattctt gtgcttcctt
      121 tgttagataa atttcaaaac aaaagacacg tatgcattct ctaattttat ttgagataaa
      181 taatgcattg ctttgttgat actgttaaat aaattacttc cctcgtaata ccaattatca
      241 tatgtttgat aaaaataatt atctttcttg atataaatat ctccccccaa tacaagtatt
      301 ctttcattta taaataaatt taatatatct attgcagtgg ggacgggtaa tgcggcttct
      361 ctaattccaa ttcctatcaa agattcccct tgtctcgaat aaatttcaaa aatttcattt
      421 aacatattaa tctctatcta ctatttttca ggaagttgtt ggttagggac aaataatcta
      481 tgattaattt ttccatctgc ttctctgata tattcaaaat ttccatcttt tcctttataa
      541 gatccaggga tataaagttt atgccttaca atattatctc cgccaactat ttgtattact
      601 gttccatttt cagaaaaagc atctactgat tgcggaaaac catgataatc accattcgat
      661 gcttgcctta atactttctc tgtataaccg gtattttcaa aaaaattatt cgttgttaca
      721 gtattttttg attcc
//
LOCUS       DAXDXW010000169          712 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXDXW010000169 DAXDXW010000000
VERSION     DAXDXW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.170"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..273
                     /locus_tag="ACFB64_002170"
     CDS             <1..273
                     /locus_tag="ACFB64_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
                     /protein_id="HFC1210434.1"
                     /translation="YFVKGNQAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQISAES
                     KIAELTNKIIPGMSKNERLKIKQKIKNIAKNANRKTKGEEHGRRGR"
     gene            254..709
                     /locus_tag="ACFB64_002171"
     CDS             254..709
                     /locus_tag="ACFB64_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210435.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     GTEYYESPDSNLLISVKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
BASE COUNT          263 a          117 c          133 g          199 t
ORIGIN      
        1 tacttcgtca agggtaatca ggcggaaacg gaaggggttt gggttcataa ttcttgtccg
       61 cctaaaagaa caggaagctc caagaatgaa aaacatggag acggcggtcg aagtcaaata
      121 tcagcggaat caaaaattgc tgaattaaca aataaaatta ttcccggaat gtccaaaaat
      181 gaacgcttaa agattaagca aaaaatcaaa aatattgcca aaaatgccaa tcgaaaaaca
      241 aaaggagaag agcatggtcg acgcggtcgt taaaactccc gaatttttac cttttacttc
      301 tgtaggaata ttccgttttg gtgctgatat aacacagtac aaaaacatac tggaaacttt
      361 tatgtatgaa cctcctgatg agtttggaac agaatattat gaatctcccg actcaaattt
      421 actcatttcc gtcaaaaaaa acaaaattat ttcgatattt tgttatcagg agctatattt
      481 tatgggcgtt aatataatag gtctgaattt tgaagatttt aaacagcttt tccatcaccc
      541 taaatcatac ggtgttgata aatattattt atcaaatgaa tcttatccga catatgtcta
      601 tgagtttgat gaaataggag tacaagcatg ggaagcaaaa gggaaaattg ttactatcat
      661 tgccgggggc aaagacaatt attcgacaga accttactac gatgaataac at
//
LOCUS       DAXDXW010000170          668 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXDXW010000170 DAXDXW010000000
VERSION     DAXDXW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.171"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(116..667)
                     /locus_tag="ACFB64_002172"
     CDS             complement(116..667)
                     /locus_tag="ACFB64_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210436.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSLWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCIIGLV
                     GRFRLQTASGRVAKAGGAVKAGG"
BASE COUNT          184 a          261 c          125 g           98 t
ORIGIN      
        1 ggtttttgtt aatccactat aaaagcaaag aaaccgccat tagcactaca cccccccaag
       61 ctgcggctgc caaacacatc ggctgcttct cgacgcaccc gctgaaaaat gcgccctatc
      121 cgcccgcctt gaccgcaccc cctgcctttg ccaccctgcc cgatgccgtc tgaagcctga
      181 accgccctac cagcccgatg atgcacgaca ccgccggaaa gggcatacac aaagccgtca
      241 gcacataacg cgcattcccc gtattgacca cgtccgcgcc cttcagcagc agcggcagcg
      301 cgttcgccca ctcccacagc gaaggcgcgc tgaacgccaa taccgccacc agccccaaca
      361 tccggaacac cccgatttcc aagacctgcc gccgccgcac cgaacggttg agcaaaaaca
      421 caaccgccaa cccgaaacag accaccgccg ccataccgcc gttggcgaca atctgcaaac
      481 tgttgaacgc aatatcgggc gtaaccgcca acacgccttc cgaatccggc acggcacgct
      541 gcaaatccaa cccctgcaac acattcaaca caaaaccgta gatcaaatca aaaaacacca
      601 cgcctatcgg caaagcactc aaaacccgcc acatcttcct atctgaccaa ccgttcaaaa
      661 ccttcatt
//
LOCUS       DAXDXW010000171          634 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXDXW010000171 DAXDXW010000000
VERSION     DAXDXW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.172"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            135..611
                     /locus_tag="ACFB64_002173"
     CDS             135..611
                     /locus_tag="ACFB64_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210437.1"
                     /translation="MEANKFEVKSLSDLIKVFAGIAADFEAAMGVKRADISTEFDEPQ
                     HEPQPPVTVAEQKGINDFAIGKEVIIRTYSAGVWFGVLEQKAGNEVILTKARRMYSWW
                     AKESISLSGVARHGIRQDGSQICGELDSVWLEAIEIIPVTGGAAESIRTALEVAQS"
BASE COUNT          200 a          145 c          162 g          127 t
ORIGIN      
        1 gcgacatgga ccactagaaa aagcgaaagc atcaatcaca ccgccttcac ttttacagtg
       61 aaaaaagacg gcttaaaaga gaaggtaatg attgttttgt aaagaaacca accccacccc
      121 gaaaggaaac aaaaatggaa gcaaataaat ttgaagtgaa aagtttgtca gaccttataa
      181 aagtctttgc aggcattgct gccgatttcg aagcggcaat gggtgtaaaa cgcgccgaca
      241 tttcaaccga atttgacgaa ccgcaacatg agccgcaacc gccggtaaca gttgccgagc
      301 aaaaaggtat caacgacttt gccatcggca aggaagtcat catccgcact tattcggcag
      361 gcgtttggtt tggtgtgttg gaacaaaaag caggcaatga agtgattctg acaaaagcgc
      421 gccgaatgta cagctggtgg gcaaaggaat caatcagcct gtcaggtgtc gcacgacacg
      481 gcatcaggca agacggcagc caaatttgcg gcgagcttga ttccgtatgg ctcgaggcga
      541 ttgagattat cccagtaaca ggcggcgcgg ctgaatcaat ccgcaccgcg ctggaggtcg
      601 cccagtcatg agttatctag atcaaccatt gaag
//
LOCUS       DAXDXW010000172          601 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXDXW010000172 DAXDXW010000000
VERSION     DAXDXW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.173"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
BASE COUNT          139 a          154 c          168 g          140 t
ORIGIN      
        1 ggcgggaatc cagtccgttc ggtttcagtc atttccaaaa attgccgtag cgttaagttt
       61 ctagattccc actttcgggg gaatgacgcg gtgcaggttt ccgtgcggac ggattcgtca
      121 ttcccacaca gacaggaatc cggatttgtc cgcgcggaaa cttatgcgcc gtcattcccg
      181 cacaggcggg aattcaggtc tgtcggcata ggaacttatc ggataaaacg gttgccccaa
      241 ccctgcgttc tagattccca ctttcgtggg aatgacgatt caggtattcc taatagcaac
      301 ccgccacaac ccacctacgc ccatcctacg catctgccat acagccctgt tccggagcgc
      361 gcggataagg tgcgatgccg ccggcgggca tcatagtcct ctctccctgt gggagagagc
      421 tagaaagctg caaggcttgt attttagaag actaagggat ttgggaaaga ttgtcgaaat
      481 tcggggagcc ctctccccca cgggggaggg ggcaggttgc ggcagatttg gcggttgcag
      541 gcggtttgaa aaacagattg gatttacaac cgttgatttc aggtcgtctg aaaagcaaaa
      601 a
//
LOCUS       DAXDXW010000173          600 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXDXW010000173 DAXDXW010000000
VERSION     DAXDXW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.174"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..501
                     /locus_tag="ACFB64_002174"
     CDS             <1..501
                     /locus_tag="ACFB64_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210438.1"
                     /translation="ASKWQVRGCSRCRADRQAGERQPRDGNGRWTAGEVVSVSGGELG
                     EFAETKELRKAAMQYARDNFVGKSYVNESSGHELKVTWQGVKHAASKSNSIELSLMVA
                     LDKVLMNAKYEGSFPNKLGRDHIIAVHKYSTGVRFGNKVLKIRIIAREKFDGIKHYDH
                     FILKDK"
BASE COUNT          186 a          110 c          159 g          145 t
ORIGIN      
        1 gcatccaaat ggcaggtgcg cggttgttcc cgttgtcggg cggacaggca agctggcgaa
       61 aggcagccgc gcgatggaaa cgggcgatgg acggcaggtg aggttgtttc cgtatcgggc
      121 ggcgaattgg gggaatttgc tgaaacaaag gagcttcgta aagcggctat gcagtatgcg
      181 cgtgataact ttgtcggcaa aagctatgtc aatgaaagca gcgggcatga actgaaggta
      241 acttggcaag gtgtgaaaca cgctgcgtca aaatcgaaca gcatcgaact ttcccttatg
      301 gtcgcgcttg ataaagtttt gatgaatgca aaatatgaag gaagttttcc taacaaatta
      361 gggagggatc atattattgc ggtacacaaa tactcaaccg gtgtcagatt tggcaataaa
      421 gtcttaaaaa tcaggataat agcccgtgag aaatttgatg ggataaaaca ttacgatcat
      481 tttattttga aagacaaatg aaaaaaccct gtccagtata tctgggatag cttatggaag
      541 catttaaccc agcctcggat aaggctttaa atccatcatg ccatttatac ggtaaaaaca
//
LOCUS       DAXDXW010000174          572 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXDXW010000174 DAXDXW010000000
VERSION     DAXDXW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.175"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            248..>572
                     /locus_tag="ACFB64_002175"
     CDS             248..>572
                     /locus_tag="ACFB64_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210439.1"
                     /translation="MTKLYAEIAEMEAQDDGTVKVWGYASSEAVDSDGEVIAAAAMKA
                     AIPDYMKFGEVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVEKVKTGVYKGFS
                     IGGSVT"
BASE COUNT          165 a          128 c          162 g          117 t
ORIGIN      
        1 aaaataggga gggatgtcgt gatatttgaa aaatcaataa acggctggaa ataccgcgcc
       61 gtaatggaaa tcagaaccac gcaaaaggag cttgccttga acagccttta tacaacagac
      121 aggattgaat agtcatgaca aaacctgcga cgagtaactg tcaatctttc gattccgtcc
      181 tcatacatcc taagcgtctt tgcaaattct gttcagttat accatttata cagacaggag
      241 caaaccaatg acgaagttat acgcagaaat cgccgaaatg gaggcgcagg acgacggcac
      301 ggtcaaagtt tggggttatg cctcaagcga ggcggtcgat tcggacggcg aagtcatcgc
      361 ggcggcggct atgaaggcgg cgattcccga ttatatgaag ttcggcgagg tgcgcgagat
      421 gcacggcagc aacgcggcgg gaacggcgat tgaaatcaac gtggaagacg acggcagaac
      481 ctttttcggg gcgcatatcg tcgatcccgt tgcagtggag aaagtcaaaa cgggcgttta
      541 caagggcttt tccatcggcg gcagcgttac cg
//
LOCUS       DAXDXW010000175          563 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXDXW010000175 DAXDXW010000000
VERSION     DAXDXW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.176"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            41..270
                     /locus_tag="ACFB64_002176"
                     /pseudo
     CDS             41..270
                     /locus_tag="ACFB64_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(307..>563)
                     /locus_tag="ACFB64_002177"
     CDS             complement(307..>563)
                     /locus_tag="ACFB64_002177"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210440.1"
                     /translation="GLYYKIWEGGWRMPALPFFRTSIPMLVQNRMESIFNHNKDAIFL
                     EGWSLGDAAMIMELLWQNISTSRRELTKLFLYKNFWGCTR"
BASE COUNT          175 a          108 c          107 g          173 t
ORIGIN      
        1 ttaacaaaaa ttaatgccct ttcttaggga cgcaatatat aaggtttata ccgtcgttgt
       61 ccctaatgcg caatcagcga cattgttgcc aattatccga aagaaagtta agcctgatgg
      121 cattgtatac acggatacct ttcgtagtta tgatgtgctt gatgttagtg aatttagcca
      181 tttacgtaag tttaacggca ttcccaaaga gcatttgggg ctatatttaa agaaatgcca
      241 atggcatttg aaatagaatc tcaaatttag attttaagac aattagttaa tgggaatttg
      301 gtctggttat ctagtacaac cccaaaagtt tttatataaa aacagtttgg taagttctct
      361 tcgtgaagtg cttatgtttt gccacaaaag ttccataatc atggcggcat cacccaagct
      421 ccatccttcc aagaaaatgg catctttgtt gtggttgaaa atcgactcca tacgattttg
      481 gacaagcata gggatagatg ttctgaaaaa cggcagcgcg ggcattctcc aaccaccttc
      541 ccaaatctta taatacaaac ccg
//
LOCUS       DAXDXW010000176          511 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXDXW010000176 DAXDXW010000000
VERSION     DAXDXW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.177"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            173..355
                     /locus_tag="ACFB64_002178"
     CDS             173..355
                     /locus_tag="ACFB64_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210441.1"
                     /translation="MANIDLTQWDGKTIGAAANPEQGYINITIGSDDLFINIEQAYAI
                     HAALGEAVAEYEGGAQ"
     gene            352..>511
                     /locus_tag="ACFB64_002179"
     CDS             352..>511
                     /locus_tag="ACFB64_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFC1210442.1"
                     /translation="MTALTLYRCAADVQAALDYYFDSETEREDTLEAVIGQFEVKAQS
                     VIAYIKNQE"
BASE COUNT          151 a          148 c          133 g           79 t
ORIGIN      
        1 gaagacatcg aggacgacat cccttttaat taagcccgcc gtcagggcgt aaaaccggac
       61 ggaaaaccgc aaacaaacgc cgcgccctgc gggcgggccc cgcactctga agcagtagtc
      121 gatttttagt ttgcaactgc ttcggacggc aacagagaaa ggaaacaaaa aaatggcaaa
      181 catcgacctg acccaatggg acgggaaaac cattggcgcc gccgccaatc ccgaacaggg
      241 ctacatcaac atcaccatcg gcagcgacga cctattcatc aacatcgaac aggcatacgc
      301 catacacgcc gcgcttggcg aagcggttgc cgaatatgag ggaggggcac aatgaccgcc
      361 ctcacactct accggtgcgc ggcagacgta caggcggcgc ttgattacta cttcgacagc
      421 gaaaccgagc gcgaagacac gctggaagcc gttatcgggc agttcgaggt caaagcgcaa
      481 tccgttatcg cttatattaa aaaccaagaa a
//
LOCUS       DAXDXW010000177          500 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXDXW010000177 DAXDXW010000000
VERSION     DAXDXW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.178"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            16..360
                     /locus_tag="ACFB64_002180"
     CDS             16..360
                     /locus_tag="ACFB64_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210443.1"
                     /translation="MIGALLKNWKPLLILSAIAFFAVSWQLDRAAQYRRGYGAAVSEV
                     SERLKAAAVEHAEHARKSSAAYQAQKAAREEKERVRYVQTLKIIEKPVYRNACFDADG
                     VRELNAAVDDGG"
BASE COUNT          101 a          139 c          170 g           90 t
ORIGIN      
        1 gcgggcgagg cgtaaatgat tggggctttg ctgaaaaatt ggaagccgct gcttattttg
       61 tccgcaatcg cgttcttcgc cgtttcttgg cagctggaca gggcggcgca ataccgtcgc
      121 ggatacggtg cggcggtgtc ggaggtttcg gaacgcctca aagccgccgc ggtcgaacac
      181 gccgaacacg cccgcaaatc gtccgccgcg tatcaggcgc aaaaggcggc gcgtgaggaa
      241 aaagaaaggg tgcgctatgt gcaaacgctt aaaatcattg aaaaacctgt gtaccgcaat
      301 gcctgttttg atgctgacgg cgtgcgcgaa ctcaacgccg ccgttgacga cggcggttaa
      361 gccgcccgcc gatttggtgc ggccctgccc gaaactgccg caccttgaag ggaacacggg
      421 cgcggacgtg ctgccgtggg ccctgaaggc ggccggtatg tataacgact gcagggcgcg
      481 gcacggcgcg ctggtacggg
//
LOCUS       DAXDXW010000178          490 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXDXW010000178 DAXDXW010000000
VERSION     DAXDXW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.179"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..463
                     /locus_tag="ACFB64_002181"
     CDS             <1..463
                     /locus_tag="ACFB64_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HFC1210444.1"
                     /translation="RTEAAPQQGGNTNPGYYKNRAFECVGFAQYLNFNLGNAFKYIWR
                     HKEKGGREDLEKALRYLERQRADAPKFKKLKCRRYEKMYAGLKDCGFDGGTEAALLAV
                     ISAAYYIRDGEDNFAWAAACVEDLLEKMPPEAGRAPHPESPMPPETAGGGI"
BASE COUNT          117 a          155 c          143 g           75 t
ORIGIN      
        1 ccggacggaa gccgcgccgc aacaaggcgg caataccaac ccgggctatt acaaaaaccg
       61 cgccttcgag tgcgtcgggt ttgcgcaata cctcaacttc aacctcggca acgccttcaa
      121 atacatctgg cggcacaagg aaaaaggcgg gcgcgaagac ttggaaaaag ccctgcggta
      181 cttggaacgc caacgcgccg acgcgccgaa gttcaagaaa ctcaaatgcc gccgctatga
      241 aaaaatgtac gccggtctga aagattgcgg gttcgacggc ggcacggagg ccgcgctgct
      301 tgccgtcatc tccgccgctt attacatccg cgacggcgaa gacaattttg cgtgggcggc
      361 cgcctgtgtc gaagatttgt tggaaaaaat gccgcctgaa gcggggcggg ccccgcaccc
      421 tgaaagcccg atgccgcctg aaacggcggg cggaggcatt tgacccgcca acccgaccgc
      481 cgccattccc
//
LOCUS       DAXDXW010000179          484 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXDXW010000179 DAXDXW010000000
VERSION     DAXDXW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.180"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..317
                     /locus_tag="ACFB64_002182"
     CDS             <1..317
                     /locus_tag="ACFB64_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LgtB"
                     /protein_id="HFC1210445.1"
                     /translation="ERLHPVDLMMFGNPDDREGMPVCQLNPALCAQELHYAKFHDQNS
                     ALGSLIEHDRRLNRKQQRRDSPANTFKHRLIRALTKIGREREKRRKRREQTIGKIIVP
                     FQ"
     gene            329..>484
                     /locus_tag="ACFB64_002183"
     CDS             329..>484
                     /locus_tag="ACFB64_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC1210446.1"
                     /translation="MDIVFAADDNYAAYLCVAAKSVEAAHPDTEIRFHVLDAGISEEN
                     RAAVAANL"
BASE COUNT          133 a          142 c          127 g           82 t
ORIGIN      
        1 ccgaacgcct gcaccctgtc gatttgatga tgttcggcaa ccctgacgac agggaaggaa
       61 tgccggtttg ccagctcaat cccgccttgt gcgcccaaga gctgcattat gccaagtttc
      121 acgaccaaaa cagcgcattg ggcagcctga tcgaacatga ccgccgcctg aaccgcaaac
      181 agcaaaggcg cgattccccc gccaacacat tcaaacaccg cctgatccgc gccttgacca
      241 aaatcggcag ggaaagggaa aaacgccgga aaaggcgcga acagacaatc ggcaagatta
      301 ttgtgccttt ccaataaaag gagaaaagat ggacatcgta tttgcggcag acgacaacta
      361 tgccgcctac ctttgcgttg cggcaaaaag cgtggaagcg gcccatcccg atacggaaat
      421 caggttccac gtcctcgatg ccggcatcag tgaggaaaac cgggcggcgg ttgccgccaa
      481 tttg
//
LOCUS       DAXDXW010000180          474 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXDXW010000180 DAXDXW010000000
VERSION     DAXDXW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.181"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..327
                     /locus_tag="ACFB64_002184"
     CDS             <1..327
                     /locus_tag="ACFB64_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC1210447.1"
                     /translation="KWYDDGVWDDEEYWKLENALIEVRKKYPYPMDIPRDIVIGIGTI
                     IDFLMVQNWKLFEIKASPWLPKSVKINERYERFRVMLRYIFTDLDAEDWKFFYFPIQH
                     SKGRLR"
     gene            358..>474
                     /locus_tag="ACFB64_002185"
     CDS             358..>474
                     /locus_tag="ACFB64_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm41 family immunity protein"
                     /protein_id="HFC1210448.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWK"
BASE COUNT          159 a           46 c          112 g          157 t
ORIGIN      
        1 aaatggtatg atgacggagt gtgggatgat gaagaatatt ggaaattgga gaatgcttta
       61 atcgaggtta gaaaaaaata tccttatccg atggatatac caagggacat cgtgattgga
      121 atcggtacca ttattgattt tttaatggtt caaaattgga aactttttga aattaaagct
      181 tctccttggt tgcctaaaag tgtcaaaatt aatgagcgtt atgaaaggtt cagagtaatg
      241 ctccgttata tttttactga tctagatgca gaggactgga aattttttta ctttccaata
      301 caacatagta aaggtagatt gagatgaaaa gtttaattat attggagata cattattatg
      361 tgtgagttca aggatattat aagaaacgtt ccttattttg aggggtatga cgaaaattca
      421 tttattggca aatggtatga tgacggggtg tgggatgatg aagaatattg gaag
//
LOCUS       DAXDXW010000181          469 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXDXW010000181 DAXDXW010000000
VERSION     DAXDXW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.182"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..237)
                     /locus_tag="ACFB64_002186"
     CDS             complement(<1..237)
                     /locus_tag="ACFB64_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFC1210449.1"
                     /translation="MTELPWIAEARRHIGLKEIPGAKHNPTIVQWLKETGGFPGAAKS
                     WYFEDETPWCGLFVGHCLGKAGRAVIRDWYRAKAW"
BASE COUNT          106 a          125 c          101 g          137 t
ORIGIN      
        1 ccaagccttg gcgcgatacc agtccctgat gaccgcgcgt cccgctttgc ccaggcagtg
       61 tccgacaaac aggccgcacc acggcgtttc gtcttcaaaa taccaagact ttgccgcgcc
      121 ggggaagccg cccgtctctt tgagccattg cacaatcgtc ggattgtgtt tcgcgccggg
      181 aatttctttc aaaccgatgt gccttctcgc ttcggctatc cacggtaatt ctgtcatttt
      241 taatcctttc ggtaaaagac gagatgtaaa ctttgggttg gcaactcgtt ctatgtttta
      301 agtcgcaatc acaatgtgca ccgcatcgcc gaacacgctg tctttttcag tcgcgtttgc
      361 cgatacggta actttccgcg cagatgatat gttgcccgtc aacacaaaat aaaaaacaaa
      421 gttgcaacat gctgatttat attgttattt ttatttacgt ttatttacg
//
LOCUS       DAXDXW010000182          464 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXDXW010000182 DAXDXW010000000
VERSION     DAXDXW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.183"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            62..464
                     /locus_tag="ACFB64_002187"
                     /pseudo
     CDS             62..464
                     /locus_tag="ACFB64_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273448.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
BASE COUNT          121 a          118 c          119 g          106 t
ORIGIN      
        1 cttcattggt atgcacgcat tcaaaaaact gacgtttgtc tttccgggat gaggtagaag
       61 catgatttat ctgtttacgg gaaacatggg ggcaggcaaa accctatgat tttgaacaac
      121 gaagacggat tgttcaaaat gaaattggaa gacggcacgg aggcagaccg gccgctttat
      181 ttctgccata tcgacggatt ggacaaacga aaattcaatg cccgcgaact ggcggaaggg
      241 caaatcatgt ccgccccgct tcgtgatgtc ataccggaag gcgcggtgct gattgttggc
      301 gaagcgcact acacttaccc ggtacgcgcg gcaggccgtc ccgttccgcc ctatattcag
      361 gaactgacag aactccgcca tcacgggcat accgtcattt tgatgacgcg gcacccgagc
      421 caacttgata tattcgtccg caaccttgtt tcaaagcatg taca
//
LOCUS       DAXDXW010000183          454 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXDXW010000183 DAXDXW010000000
VERSION     DAXDXW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.184"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>454
                     /locus_tag="ACFB64_002188"
     CDS             <1..>454
                     /locus_tag="ACFB64_002188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC1210450.1"
                     /translation="VNILPFSKVKGLVKAAKLGRAAKPGRAAKLGKAKVSEDFAVASQ
                     ARYTSQTAENIIAADRIGSGLKADTSHRAASFLSQEQLGAGRTFSITGGDGIQRTLLQ
                     TKGSLNGQSGIYEYILDSNNKVTHQRFIKGGEITGQPNQRVKTKVRNRG"
BASE COUNT          156 a           84 c          118 g           96 t
ORIGIN      
        1 tggtcaacat cctgccgttt tccaaagtca aaggattggt gaaggcggcg aaactgggga
       61 gggcggcaaa accggggagg gctgcgaaac tagggaaggc aaaggttagc gaggattttg
      121 ctgttgccag tcaggcaaga tatacttcgc aaacggcaga aaatataatt gcagccgata
      181 gaataggaag tggattaaaa gctgatacgt cacatcgtgc agccagcttt ctatctcaag
      241 aacaattggg agcaggaaga acattctcaa ttactggcgg cgatggtatt caaagaactt
      301 tattgcagac aaaaggttct ttgaacggtc aaagcggtat atatgaatat atcctcgatt
      361 caaataacaa agtgactcat caaagattca ttaaaggagg tgaaattaca ggacaaccaa
      421 accaacgcgt gaaaactaaa gtacgcaacc gggg
//
LOCUS       DAXDXW010000184          424 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXDXW010000184 DAXDXW010000000
VERSION     DAXDXW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.185"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(1..317)
                     /locus_tag="ACFB64_002189"
                     /pseudo
     CDS             complement(1..317)
                     /locus_tag="ACFB64_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           85 a          122 c          106 g          111 t
ORIGIN      
        1 gccttacgca ccgcagacct tccgccgaag cagcggcttt tgaatttggt ttccccgctc
       61 tccctcgggt gcggggcaat gccgaccaaa cccgctatcc gtttgtgcga cagccgcccc
      121 aattcgggca gcatcgccat cagcgtagcc gtcgttatcg aaccgatgcc tttgatttgc
      181 tccgccactt gggctttgcc gtcaaaatgc gtgcgggtgt ggttgtcgat ttgtttgtcc
      241 gattcgtcaa tcagccggtc aaaacgggca atcagttgtt tgacgctttc gacttgcgtt
      301 tcgtgaacct gatgcagaca ttttgtaata gctgccatta cgcctactag gatatatacc
      361 ttctatatat agtgaattaa caaaaaccgg tacggcgttg ccccgccccg gctcaaaggg
      421 aacg
//
LOCUS       DAXDXW010000185          420 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXDXW010000185 DAXDXW010000000
VERSION     DAXDXW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.186"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>420
                     /locus_tag="ACFB64_002190"
     CDS             <1..>420
                     /locus_tag="ACFB64_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HFC1210451.1"
                     /translation="ITDDQDKQKHFFMFGAMGLGGRGAYALDLSKIDSSNLTGVSMFD
                     VQNDKNNNNNKNDNNRVKLGYTVGTPQIGKTQNGKYAAFLASGYAAKKIDDSTNKTAL
                     YVYDLGNGSGSLIKKIEVQGGKGGLSSPTLVDKDLDGT"
BASE COUNT          133 a           96 c          103 g           88 t
ORIGIN      
        1 cattacagat gaccaagaca agcaaaaaca tttctttatg tttggtgcga tgggccttgg
       61 cggcagaggc gcgtatgcct tggatttaag caaaatcgac agcagcaacc tgaccggcgt
      121 ttccatgttt gatgtccaaa acgacaaaaa taacaataac aataagaatg acaataatcg
      181 cgtgaaatta ggctacaccg tcggcacgcc gcaaatcggc aaaacccaaa acggcaaata
      241 cgccgccttc ctcgcttccg gttatgcggc taaaaaaatt gacgactcaa caaataaaac
      301 cgcgctgtat gtgtatgatt tgggaaacgg cagtggtagt ctgattaaaa aaatcgaagt
      361 gcagggcggc aagggcgggc tttcgtcccc cacgctggtg gataaagatt tggacggcac
//
LOCUS       DAXDXW010000186          418 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXDXW010000186 DAXDXW010000000
VERSION     DAXDXW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.187"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>418
                     /locus_tag="ACFB64_002191"
     CDS             <1..>418
                     /locus_tag="ACFB64_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC1210452.1"
                     /translation="KYSVNIENVQIREDNGNRIDRKTENQENGTFHAVSSLGLSAVYD
                     FKLNDKFKPYIGARVAYGHVRHSIDSTKKTTEFLTSAGAPGGAPTVYHPHKNTQDDHR
                     EGDSIRRVGLGAVAGVGIDITPNLTLDAGYRYHNWGR"
BASE COUNT          117 a          127 c           99 g           75 t
ORIGIN      
        1 taaatattcc gtcaacatag aaaatgtgca gatacgtgaa gacaatggca acaggataga
       61 ccggaagacg gaaaatcagg aaaacggcac attccacgcc gtttcttctc tcggcttgtc
      121 cgccgtttac gatttcaaac tcaacgacaa attcaaaccc tatatcggtg cgcgcgtcgc
      181 ctacggacac gtcagacaca gcatcgattc gaccaaaaaa acaacagagt ttcttacctc
      241 cgccggtgct cctggcggag cgcctacggt ttatcatcct cacaaaaata cgcaagacga
      301 ccatcgcgaa ggcgacagca tccgccgcgt gggcctcggc gcagtggcag gcgtaggcat
      361 cgacatcacg cccaacctga ccttggacgc cggctaccgc taccacaact ggggacgc
//
LOCUS       DAXDXW010000187          417 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXDXW010000187 DAXDXW010000000
VERSION     DAXDXW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.188"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..>417)
                     /locus_tag="ACFB64_002192"
     CDS             complement(<1..>417)
                     /locus_tag="ACFB64_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication initiation protein, NGO0469
                     family"
                     /protein_id="HFC1210453.1"
                     /translation="MPDGRPYLISRRYTASLHSKSQLATDLKSWRGRDFTPEERDNFD
                     LRNILGKPCLLSIAHQESSDGKTTYANISAISNKMKSYTPKQPDNAVFAFDLSDPDWA
                     NYGLLNEKLREQIAKSPEYAEAVNGRQPPAPPQKQAQ"
BASE COUNT           57 a          104 c          137 g          119 t
ORIGIN      
        1 ttgggcctgt ttctgcggcg gcgccggcgg ttggcggccg tttacggctt cggcatattc
       61 cgggcttttg gcaatctgct ccctcagttt ctcgttcagg aggccgtaat tcgcccaatc
      121 ggggtctgac aggtcgaagg caaaaacggc gttgtccggc tgtttcgggg tgtagctctt
      181 catcttgttg ctgatggcgg aaatgttggc ataggtggtt ttgccgtcgc tgctttcttg
      241 gtgggcgata ctcaacaggc agggcttgcc caaaatattg cgcaaatcga agttgtcgcg
      301 ttcttccggt gtaaagtccc ttccgcgcca gcttttgagg tctgttgcca gttggctttt
      361 gctgtgcagg ctggcggtgt accggcggct gatgaggtag ggcctgccgt ccggcat
//
LOCUS       DAXDXW010000188          414 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXDXW010000188 DAXDXW010000000
VERSION     DAXDXW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.189"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>414
                     /locus_tag="ACFB64_002193"
     CDS             <1..>414
                     /locus_tag="ACFB64_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HFC1210454.1"
                     /translation="VELRGQKHVFMFGAMGLGGRGAYALDLSKINGNYPAAAPLFDVK
                     NGDKNGKNGKNRVEVELGYTVGTPQIGKTQNGKYAAFLASGYAAKQIASQENTTALYV
                     YDLENTLGTPIAKIDVPGGKGGLSSPTLVDKDLDGT"
BASE COUNT          115 a          105 c          114 g           80 t
ORIGIN      
        1 agtcgaactg cgcgggcaaa aacacgtgtt tatgttcggc gcgatgggtt tgggcggcag
       61 gggcgcgtat gccttggatt taagcaaaat caacggaaat tatccggccg ccgcccccct
      121 gtttgatgtc aaaaatggcg ataaaaacgg caaaaacggc aaaaatcgcg tggaagtgga
      181 attaggctac accgtcggta cgccgcaaat cggcaaaacc caaaacggca aatacgccgc
      241 cttcctcgcc tccggttatg cggctaaaca aattgccagc caagaaaata caaccgcgct
      301 gtatgtgtat gatttggaaa acaccttagg tacgccgatt gcaaaaatcg atgtgcccgg
      361 cggcaagggc gggctttcgt cccccacgct ggtggataaa gatttggacg gcac
//
LOCUS       DAXDXW010000189          407 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXDXW010000189 DAXDXW010000000
VERSION     DAXDXW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.190"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(51..>407)
                     /locus_tag="ACFB64_002194"
     CDS             complement(51..>407)
                     /locus_tag="ACFB64_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC1210455.1"
                     /translation="TIYDFDTGSRFKPYIGMRVAYGHVRHQVRSVQQETEIVTTYPSD
                     GSAKTSVPSEMPPKPAYHENRSSSRLGFGAMAGVGIDVAPGLTLDAGYRYHYWGRLEN
                     TRFKTHEASLGVRYRF"
BASE COUNT           86 a          101 c          131 g           89 t
ORIGIN      
        1 gcgcatcaaa aatgccgtct gaaggttcag acggcatcgg aatcggcgaa tcagaagcgg
       61 tagcgcacgc ccaacgaggc ttcgtgggtt ttgaagcggg tgttttccag gcgtccccaa
      121 tagtggtagc ggtagccggc gtccaaggtc aggccgggcg cgacgtctat gcccacgccc
      181 gccatcgcgc cgaagcccaa gcggctgctg ctgcggtttt cgtgataggc aggtttgggg
      241 ggcatttctg atggaacaga agttttcgca ctaccgtcgc ttgggtaagt ggtaacaatc
      301 tcagtttctt gttgaaccga acgaacctga tgtctgacgt gtccgtaggc gacgcgcatg
      361 ccgatatagg gtttgaagcg ggaaccggta tcgaaatcgt aaatggt
//
LOCUS       DAXDXW010000190          404 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXDXW010000190 DAXDXW010000000
VERSION     DAXDXW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.191"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>404
                     /locus_tag="ACFB64_002195"
     CDS             <1..>404
                     /locus_tag="ACFB64_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HFC1210456.1"
                     /translation="GTGSDLSESDVFNTDEQYIYGIFDDDKANVSVKVKDHMGDGLLK
                     QNLTQENKTLFLTNNKASGGSADKGWVVKLKEGQRVTVKPTVVLRTAFVTIRSYTGND
                     KCGAQTAILGINTADGGALTPRSARPIVPGDQ"
BASE COUNT          115 a           95 c          114 g           80 t
ORIGIN      
        1 tcggtacggg cagcgatttg agtgaaagtg acgtattcaa tacggacgaa caatatattt
       61 acggtatctt tgacgacgat aaggcgaatg ttagtgtaaa ggtaaaagac cacatgggcg
      121 acgggctgct caagcaaaac cttacgcagg aaaataaaac cttgttcctg acaaataata
      181 aggcatccgg cggatcggcc gataaaggct gggtagtgaa attgaaggaa ggacagcgcg
      241 ttaccgtcaa accgaccgtg gtattgcgta ccgcctttgt caccatccgc agctatacgg
      301 gtaatgacaa atgcggcgcg caaaccgcca ttttgggcat caataccgcc gacggcggcg
      361 cattgactcc gagaagcgcg cgcccgattg tgccgggtga ccag
//
LOCUS       DAXDXW010000191          399 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXDXW010000191 DAXDXW010000000
VERSION     DAXDXW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.192"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
BASE COUNT           99 a          113 c          122 g           65 t
ORIGIN      
        1 ccattgccgc gcaggcggca atggcgggcc gggcaaacgg ttgcggattg ccaaaaaccg
       61 ctgtaccatg gataagcgcg caagggtaga ataatgcggt aaccctatac attgcatccg
      121 gccaaagggg ttttccaacc ggcaatccgg cgggcgcggg atcgggcggt ttgccgaatc
      181 ccgccgtttg cggcgcgcct gccgccgacg gtatcccgcg aagcaagatt taagggataa
      241 aatatgttcc aacacacagg gcggcacata aggcgccgcc ctgattcgga agggcttgca
      301 cccctcccgg acaaagcctg atcctgccgc cccgaaggac ggatgtccga gcggcggggt
      361 ttcaaccgaa aaggaaatac gatgaataaa actttgaaa
//
LOCUS       DAXDXW010000192          393 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXDXW010000192 DAXDXW010000000
VERSION     DAXDXW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.193"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            99..393
                     /locus_tag="ACFB64_002196"
                     /pseudo
     CDS             99..393
                     /locus_tag="ACFB64_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          117 a          110 c           93 g           73 t
ORIGIN      
        1 aacctacatc ccgtcattcc cacgaaagtg ggaatccgga acgcaaaatc taaagaaacc
       61 gttttacccg ataagtttcc gcactgacag acccggattc ccgcctgcgc gggaatgacg
      121 aagccatcct tttggccgaa ggtcaaaaat cagccgttac cgggtattac ctgaatcacg
      181 gcacatggcc gaaagacaac acttctgccg gcgtggcatc ctcccccacc gacatcaaag
      241 gcaaatatgt taaaagcgtt acggtcgcaa aaggcgtcgt taccgcccaa atggcttcaa
      301 ccggcgtaaa caaagaaatc aaagacaaaa gactctccct gtggggcagg cgtgaaaacg
      361 gttcggtaaa atggttctgc ggacagccgg tta
//
LOCUS       DAXDXW010000193          384 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXDXW010000193 DAXDXW010000000
VERSION     DAXDXW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.194"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
BASE COUNT          130 a           89 c           62 g          103 t
ORIGIN      
        1 tgttaatcca ctataaaagg tcttttctaa aatatgcaga tattcgaatg tcgaatttgc
       61 cttatgatta cggttttctg tcttatccgc cttaaaacgc cggtattccg tttgaaccaa
      121 attatatttg ccgaaacact caaaaatctc ccttacctgc ccgacagaca tcaagacttc
      181 gttgttgtaa ctgagaaaaa tatattgaaa gtccgcatcc ctaatcaaag cctcaaatac
      241 cggaaccaca tccgattttg agcaagtccg cgctaaaaca catcgtacag cgcaaaaccg
      301 gatgggaatg gcaaaatccg gaaacaaaca tttttcaaag cgtacaaaca aaaaatacca
      361 accggaggtt ggtatttctt aact
//
LOCUS       DAXDXW010000194          372 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXDXW010000194 DAXDXW010000000
VERSION     DAXDXW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.195"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
BASE COUNT           90 a           95 c           98 g           89 t
ORIGIN      
        1 agggcggtgc tttatgtgtc gtcctgtgtg ttgaaacata tcggacgcgt ttgaacagtt
       61 gtgcggcaaa ctgttttagc ggaaggcaag agatgccgtc tgaagcccat tgtccgtcat
      121 tcccacacag gcagttgcta cggttgctga agccccttag ccgtcgcgca ggcagttgtt
      181 acggttacta aaatcccgtc atttcctcaa aaacagaaaa ccaaaatcag aaacctaaaa
      241 tcccgtcatt cccgcgcagg cgggaatccg ggcctgtcgg cacggaaact tatcggataa
      301 aacggtttct ttagatttta cgttccggat tcccgcctgc gcgggaatga cggcgggaga
      361 acaatgccgt ct
//
LOCUS       DAXDXW010000195          368 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXDXW010000195 DAXDXW010000000
VERSION     DAXDXW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.196"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>368
                     /locus_tag="ACFB64_002197"
     CDS             <1..>368
                     /locus_tag="ACFB64_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HFC1210457.1"
                     /translation="SYTGCPKLVYKTRFTFGQQGLKRKAGSKLDIYEDKSRENSPIYK
                     LSDYPWLGVSFNLGSENTVQNSKLFNKLISSFREGNNNQTIVSTTEGNPISLGDRQRE
                     HTAVAYYLNAKLHLLDKKGI"
BASE COUNT          120 a           87 c           76 g           85 t
ORIGIN      
        1 aagctacaca ggctgtccca aacttgtcta taaaacccga tttaccttcg gtcaacaagg
       61 gttgaaaaga aaggcaggca gcaagctgga tatatacgaa gacaaaagcc gcgaaaattc
      121 gcccatttac aaattgtcgg attatccttg gttgggcgta tctttcaatt tgggcagcga
      181 gaataccgtc caaaatagca aattattcaa caaattgata tcttctttta gagaaggcaa
      241 taataatcaa accatcgtct ctacgacaga aggcaaccct atttcccttg gcgaccggca
      301 gcgcgaacat accgccgtgg cctattatct gaacgccaaa ctgcacctgc tggacaaaaa
      361 agggattg
//
LOCUS       DAXDXW010000196          365 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXDXW010000196 DAXDXW010000000
VERSION     DAXDXW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.197"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..>365)
                     /locus_tag="ACFB64_002198"
     CDS             complement(<1..>365)
                     /locus_tag="ACFB64_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210458.1"
                     /translation="FKFNPLYMAGGVYLGGKIISPIYHSSQEFSGEPIIYAETNIILC
                     PAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVRFDKNYDKYKTWQVAIEKAANFLK
                     LGNPYNEDKLSLSEEHDTI"
BASE COUNT          126 a           53 c           47 g          139 t
ORIGIN      
        1 aatagtgtca tgttcttcac taagagataa tttatcttca ttatatggat taccgagttt
       61 taaaaaattt gctgcttttt ctatagcaac ttgccaagtt ttatatttat cgtaattttt
      121 atcaaaacgt actccccctt gtttattgga taaaaaagta attatctgat tcatattaaa
      181 aatatttcca ttatggaaaa ctcgttttaa agttaaaaat ttttcagcag gacataaaat
      241 tatatttgtt tcagcataga ttattggttc accagaaaat tcttgtgatg aatgatatat
      301 agggctaata atttttccgc ctaaataaac tccaccagcc atatagagtg gattaaattt
      361 aaacc
//
LOCUS       DAXDXW010000197          364 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXDXW010000197 DAXDXW010000000
VERSION     DAXDXW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.198"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>364
                     /locus_tag="ACFB64_002199"
     CDS             <1..>364
                     /locus_tag="ACFB64_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC1210459.1"
                     /translation="SKYSVSIKELKNNNNKKTENQENGSFHAVSSLGLSAVYDFKLND
                     KFKPYIGARVAYGHVRHSIDSTKKITAGARGAGATVHPPYKSTQDAHHQSNSIRRVGL
                     GVIAGVGFDITPKLTLDTG"
BASE COUNT          108 a          106 c           80 g           70 t
ORIGIN      
        1 acagtaaata ttccgtcagc ataaaagagt tgaaaaataa taataataag aagacggaaa
       61 atcaggaaaa cggcagcttc cacgccgttt cttctctcgg cttatcagcc gtttacgatt
      121 tcaaactcaa cgacaaattc aaaccctata tcggcgcgcg cgtcgcctac ggacacgtca
      181 gacacagcat cgattcgact aaaaaaataa cagccggtgc tcgtggcgca ggggctacgg
      241 ttcatcctcc ttacaaaagt acgcaagacg cccatcacca aagcaacagc atccgccgcg
      301 tgggtctcgg cgtcatcgcc ggcgtcggtt tcgacatcac gcccaagctg accctggaca
      361 ccgg
//
LOCUS       DAXDXW010000198          364 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXDXW010000198 DAXDXW010000000
VERSION     DAXDXW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.199"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
BASE COUNT          127 a          102 c           88 g           47 t
ORIGIN      
        1 cccgaaaccg cttacaccgg cggcggagaa acagagccgt acggaccaag ccccgaagaa
       61 atcaaatacc ggcaaagccc ggaaaccgcc gaaacacggc ggatgaccga aaaacaggca
      121 gaaggccaca ttaaaagcat tatcaggtaa cgccgccgcg caaggcataa aacagcgcga
      181 gaccgataga cgaaacgacc tttaacagga gaccgaaaat gtcattgatt ttaagtgtga
      241 aagacgaaag caatttcaaa ccatgcccgg caggcagcca tcacgccacc tgcatccgca
      301 tcatcgattt gggtacgcag ctcgtcgagt accaaaacga acaaaagcgg cagcacaaga
      361 tttt
//
LOCUS       DAXDXW010000199          361 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXDXW010000199 DAXDXW010000000
VERSION     DAXDXW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.200"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..243
                     /locus_tag="ACFB64_002200"
     CDS             <1..243
                     /locus_tag="ACFB64_002200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210460.1"
                     /translation="ATGANQGKISTVSDYFKNIRTRSVHPRLALGYDFGGWRVFSAPA
                     VAAGLHQSLQYYRSRHCRRFRQIHQFRRITPIPQKP"
BASE COUNT           82 a          144 c           68 g           67 t
ORIGIN      
        1 gcaaccggtg caaaccaagg caaaataagc acggtaagcg attatttcaa aaacatccgc
       61 acccgctccg tccacccccg actcgccctc ggctacgatt tcggcggctg gcgcgtcttt
      121 tccgcccccg ccgttgccgc cggcctccac caatcccttc aatattaccg atcccgccat
      181 tgccgccgat tccgtcaaat ccatcaattc cgccgaatca cgcctattcc ccaaaaacct
      241 tgatgcggcg ggctgaagcc cgccctgcaa ccctctctat gcaccccctt gcgagcccga
      301 cactacgcaa catcttgaga acccatcctg tcaagaatac ccgaaccgtc ccgatacacc
      361 g
//
LOCUS       DAXDXW010000200          355 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXDXW010000200 DAXDXW010000000
VERSION     DAXDXW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.201"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>355
                     /locus_tag="ACFB64_002201"
     CDS             <1..>355
                     /locus_tag="ACFB64_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC1210461.1"
                     /translation="SSSKSKRELKTVNQENGTFHAVSSLGLSAVYDFKLNDKFKPYIG
                     ARVAYGHVRHSIDSTKKITGLLTTTSTRGAEPGSYKVSTTPGAHQESNSIRRVGLGVI
                     AGVGFDITPKLTLDAG"
BASE COUNT           98 a          103 c           87 g           67 t
ORIGIN      
        1 aaagtagcag taagagtaag agagaactga agacggtaaa tcaggaaaac ggtacgttcc
       61 acgccgtttc ttctctcggc ttgtccgccg tttacgattt caaactcaac gataaattca
      121 aaccctatat cggcgcgcgc gtcgcctacg gacacgtcag acacagcatc gattcgacta
      181 aaaaaataac agggcttctt accaccacca gtactcgtgg cgcagagcct gggagttata
      241 aggtatcaac gacaccaggc gcccatcaag aaagcaacag catccgccgc gtgggcctcg
      301 gtgtcatcgc cggcgtcggt ttcgacatca cgcccaagct gaccctggac gccgg
//
LOCUS       DAXDXW010000201          333 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXDXW010000201 DAXDXW010000000
VERSION     DAXDXW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.202"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
BASE COUNT           85 a           99 c           65 g           84 t
ORIGIN      
        1 ccgttcggtt tcagttattt ccgataaatt cctgctgctt tttatttcta gattcccact
       61 tccgtgggaa tgacggcgga ggggataagt tcttgcaatc taaaatttcg tcatttctat
      121 aaaatagcaa accgaaacag aaacttaaaa acagcaacct gaaacagcaa cctgaaaccc
      181 cgtcattccc gcgcaggcgg aaatccggcg ggccgtaggg tgtgcttcgg cccgccattt
      241 ccatcaatcc aacatgtcta ccgttttcat cgaatccatc gaatccgcct tttcgaccac
      301 ccggccctac gcaaccgaac cgtcattccc gcg
//
LOCUS       DAXDXW010000202          320 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXDXW010000202 DAXDXW010000000
VERSION     DAXDXW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.203"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..>320)
                     /locus_tag="ACFB64_002202"
     CDS             complement(<1..>320)
                     /locus_tag="ACFB64_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210462.1"
                     /translation="ARDYAIIMNERNQPDVRWDGQYQKSQLREKDRERKFIYNKGRQG
                     GGSVFFDNTDELVSQQSGTAVFGTATYLPPYGKVSGFDADGLKKRGNAVNWIRTTRPG
                     LAGY"
BASE COUNT           69 a           85 c           77 g           89 t
ORIGIN      
        1 cgtagcctgc cagcccgggc cgggtggtac gaatccaatt gacggcattg ccgcgctttt
       61 tcagcccgtc ggcatcaaaa ccggaaacct tgccgtaggg cggcaggtag gtggctgtgc
      121 caaaaacggc agtaccgctt tgttgagaaa caagctcatc ggtattgtcg aaaaagacag
      181 agcctccacc ctgtctgcct ttattataga taaatttgcg ttccctgtct ttttccctta
      241 attgtgattt ttgatattgc ccatcccacc ttacatccgg ctgatttcgt tcgttcatga
      301 taatagcgta gtcacgggct
//
LOCUS       DAXDXW010000203          318 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXDXW010000203 DAXDXW010000000
VERSION     DAXDXW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.204"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..>318)
                     /locus_tag="ACFB64_002203"
     CDS             complement(<1..>318)
                     /locus_tag="ACFB64_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HFC1210463.1"
                     /translation="AQTHQYAIIMNERNQPEVKQNVPSSIKDKDRRREYTYYTHRTGG
                     GSVSFNNNDTLVSQQSGTAVFGTATYLPPYGKVSGFDADALKERNNAVDWIRTTRIAL
                     AGY"
BASE COUNT           67 a           73 c           88 g           90 t
ORIGIN      
        1 agtagcctgc cagcgcgatg cgggtggtac gaatccaatc aacggcattg ttgcgctctt
       61 tcagagcgtc ggcatcaaaa ccggaaacct tgccgtaggg cggcaggtag gtggctgtgc
      121 caaaaacggc agtaccgctt tgttgggaaa caagggtatc gttattgttg aatgagacag
      181 agcctcctcc tgttctgtgc gtataataag tatattcgcg cctcctgtct ttgtccttta
      241 ttgaagatgg cacattctgc tttacctcgg gctggtttcg ctcgttcatg ataatagcgt
      301 attgatgggt ttgcgcca
//
LOCUS       DAXDXW010000204          318 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXDXW010000204 DAXDXW010000000
VERSION     DAXDXW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.205"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>318
                     /locus_tag="ACFB64_002204"
     CDS             <1..>318
                     /locus_tag="ACFB64_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFC1210464.1"
                     /translation="VARCGLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQ
                     ELEALPGIGPAKAKAIAEYRAQNGAFKSVDDLIKVKGIGPAVLAKLKDQASVGAPAPK
                     GPAK"
BASE COUNT           72 a           84 c           98 g           64 t
ORIGIN      
        1 gtcgcaagat gcggtttgtt gtttgcaacc cttaaaggaa aaaccatgaa aaaaatgttt
       61 gtattgttct gtatgctgtt ctcctgcgcc ttctcccttg cggcggtaaa catcaatgcg
      121 gcttcgcagc aggagctgga ggcgctgccg ggcataggcc cggcgaaggc gaaggccatt
      181 gcggaatacc gcgcgcaaaa cggcgcgttc aagtctgtgg acgatttgat caaggtgaag
      241 ggcatcggtc cggcggtgct ggcgaagctg aaagaccagg cttccgtcgg cgcgcccgca
      301 ccaaaaggcc ccgccaaa
//
LOCUS       DAXDXW010000205          312 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXDXW010000205 DAXDXW010000000
VERSION     DAXDXW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.206"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
BASE COUNT           83 a           81 c           84 g           64 t
ORIGIN      
        1 gcgggctata gggtaggctt catcccgcca atctcactga atccgtcaat ttccgcaatt
       61 caattaaata ccgtcaaacc gatgccgtca ttcccgcgca ggcgggaatc cggaccggtc
      121 gggcatctgc ggcggtttgc taaaaaaacg ctttaccgtg ataagtgcgc aaagttaaaa
      181 tggggaggta agcttttcaa tcagcaatcc ggcgggcgcg gaatcgggcg gtttaccgaa
      241 ccccggcgtt cgcggcgcgc cgtcccgcga aggcaaactt aaggaataaa atatgaataa
      301 aactttgaaa cg
//
LOCUS       DAXDXW010000206          294 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXDXW010000206 DAXDXW010000000
VERSION     DAXDXW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.207"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..222
                     /locus_tag="ACFB64_002205"
     CDS             <1..222
                     /locus_tag="ACFB64_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210465.1"
                     /translation="FNAVPRKEGGVYYYKSCKTCRNKAVRQKRAEKRAAAGAGAMTAA
                     RLHGYIRAAHAACPILGAGLWTQPAGECA"
     gene            213..>294
                     /locus_tag="ACFB64_002206"
     CDS             213..>294
                     /locus_tag="ACFB64_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFC1210466.1"
                     /translation="MRVIRLILPYPVSANRYWRIWRNRAVR"
BASE COUNT           64 a           88 c           99 g           43 t
ORIGIN      
        1 tttaatgccg tcccgcgcaa ggaagggggg gtctattatt acaaatcgtg caaaacctgc
       61 cgcaacaagg cagtccggca aaagcgcgcg gaaaaacgcg cggcggcggg agccggcgcg
      121 atgacggcgg caaggctgca cggatacatc cgcgccgcgc acgccgcctg cccgatattg
      181 ggcgccggcc tgtggacgca accggcaggg gaatgcgcgt gatacgcctt atcctgcctt
      241 accccgtatc ggcaaaccga tattggcgga tttggcgcaa cagggcggtc agga
//
LOCUS       DAXDXW010000207          292 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXDXW010000207 DAXDXW010000000
VERSION     DAXDXW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.208"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..168
                     /locus_tag="ACFB64_002207"
     CDS             <1..168
                     /locus_tag="ACFB64_002207"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFC1210467.1"
                     /translation="KTVIDLDNALKVALDALQGVAYHNDRQVRRIAADYADEPVAGGG
                     LAVEVGELDEK"
BASE COUNT           65 a           69 c          103 g           55 t
ORIGIN      
        1 aagacggtga tcgatttgga caacgccctg aaggttgcgc tggacgcgct tcaaggcgtt
       61 gcctatcaca acgacaggca ggtgcggcgc attgccgccg attacgccga cgagccggtc
      121 gcaggcggcg gtttggcggt ggaggtgggg gaattggatg aaaagtaaaa ccgaagcgga
      181 aaaatcacat ctgcaaaaag tggcggatat aggttgtatt gtttgccgca attgcgggcg
      241 gttcggcgtt cctgccgagg tccgccatat ccgaaacggt gcaggcgcgg gc
//
LOCUS       DAXDXW010000208          275 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXDXW010000208 DAXDXW010000000
VERSION     DAXDXW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.209"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>275
                     /locus_tag="ACFB64_002208"
     CDS             <1..>275
                     /locus_tag="ACFB64_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC1210468.1"
                     /translation="ASKIIGKYVQSVTVTNGVVTAQMNPSGVNNEIKGKKLSLWAKRE
                     NGSVKWFCGQPVKRDAGAKTGADDVKADGKDTDKINTKHLPSTCRDK"
BASE COUNT           91 a           80 c           65 g           39 t
ORIGIN      
        1 ccgcttcaaa aatcataggc aaatatgttc aaagcgttac ggtcacaaac ggcgtcgtta
       61 ccgcccaaat gaatccaagc ggcgtaaaca atgaaatcaa aggcaaaaaa ctctccctgt
      121 gggccaagcg tgaaaacggt tcggtaaaat ggttctgcgg acagccggtt aagcgcgacg
      181 ccggcgccaa aaccggcgcc gacgacgtca aagccgacgg caaagacacc gacaaaatca
      241 acaccaagca cctgccgtca acctgccgcg ataaa
//
LOCUS       DAXDXW010000209          272 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXDXW010000209 DAXDXW010000000
VERSION     DAXDXW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.210"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
BASE COUNT           73 a           81 c           53 g           65 t
ORIGIN      
        1 tcctcgcctt gtcctgattt ttgttaatcc actataatca ggaaagacaa aaaaccttcc
       61 gccgtcattc ccgcgcaggc gggaatccga tccgttggga ttttgcaact tcaaatcaat
      121 ccgcaaactg aaatcccgtc attcccgcgc agtcgtgaat ccgaacgcgt ccgcacggaa
      181 atccgcatcc cgtcattccc acgaaagtgg gaatctagaa tgtcaaatct caagaaaccg
      241 ttttatccga taagttccta tgccgacgga cc
//
LOCUS       DAXDXW010000210          271 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXDXW010000210 DAXDXW010000000
VERSION     DAXDXW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.211"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>271
                     /locus_tag="ACFB64_002209"
     CDS             <1..>271
                     /locus_tag="ACFB64_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC1210469.1"
                     /translation="WPENNASAGVASSPTDIKGKYVKEVEVKNGVVTAKMLSSGVNKE
                     IQGKRLSLWAKRENGSVKWFCGRPVKRAEAKAGNDDVTKDDANAI"
BASE COUNT           85 a           75 c           70 g           41 t
ORIGIN      
        1 aatggccgga aaacaacgct tctgccggcg tggcatcctc ccccaccgac atcaaaggca
       61 aatatgttaa agaggttgaa gttaaaaacg gcgtcgttac cgccaaaatg ctttcaagcg
      121 gcgtaaacaa agaaatccaa ggcaaaagac tctccctgtg ggccaagcgt gaaaacggtt
      181 cggtaaaatg gttctgcgga cggccggtta agcgcgccga agccaaagcc ggcaacgacg
      241 acgtcaccaa agacgacgcc aacgccatcg a
//
LOCUS       DAXDXW010000211          270 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXDXW010000211 DAXDXW010000000
VERSION     DAXDXW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.212"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..>270)
                     /locus_tag="ACFB64_002210"
     CDS             complement(<1..>270)
                     /locus_tag="ACFB64_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC1210470.1"
                     /translation="EKLGKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVI
                     VYLKYRKDDHFSVLRGVDGNTVLLADPSPGHVSMSRAQFLEAWQTR"
BASE COUNT           68 a           85 c           61 g           56 t
ORIGIN      
        1 cgggtttgcc aagcctccaa aaactgcgcc ctgctcatcg aaacatgacc cggcgacggg
       61 tcggcaagca aaaccgtatt gccatccact ccgcgcaata ccgaaaaatg gtcgtctttg
      121 cggtatttca gatacacgat gacggggatt ttcaactgcg cgagctgctc gaaagacagg
      181 gcatagccct tcgcctcaaa acccaaatcg ggcataatgc gccgcatatc ctcaaacgac
      241 gcgcgcatct gttccttacc cagtttttcc
//
LOCUS       DAXDXW010000212          269 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXDXW010000212 DAXDXW010000000
VERSION     DAXDXW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.213"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>269
                     /locus_tag="ACFB64_002211"
     CDS             <1..>269
                     /locus_tag="ACFB64_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC1210471.1"
                     /translation="NNTSAGVASSATDIKGKYVKEVKVENGVVTATMASSNVNKEIKD
                     KRLSLWAKRENGSVKWFCGQPVTRGAGNAGKADDVTKAGNDNEKI"
BASE COUNT           90 a           75 c           66 g           38 t
ORIGIN      
        1 aaaacaacac ttctgccggc gtggcatcct ccgccaccga catcaaaggc aaatatgtta
       61 aggaagttaa agtcgaaaac ggcgtcgtta ccgccacaat ggcttcaagc aacgtaaaca
      121 aagaaatcaa agacaaaaga ctctccctgt gggccaagcg tgaaaacggt tcggtaaaat
      181 ggttctgcgg acagccggtt acgcgcggcg ccggcaacgc cggcaaagcc gacgacgtca
      241 ccaaagccgg caacgacaac gaaaaaatc
//
LOCUS       DAXDXW010000213          269 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXDXW010000213 DAXDXW010000000
VERSION     DAXDXW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.214"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>269
                     /locus_tag="ACFB64_002212"
     CDS             <1..>269
                     /locus_tag="ACFB64_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC1210472.1"
                     /translation="CDYFPEILACQEMGKPSDRMFHDISIPQVTDDKTWSSHNFLPSN
                     GVCPQPKTFHVFGRQYRASYEPLCVFAEKIRFAVLLAFIIMSAFV"
BASE COUNT           64 a           66 c           60 g           79 t
ORIGIN      
        1 cgtgcgatta ttttccggaa atcctagcct gtcaggagat gggcaaacct tcggaccgca
       61 tgtttcacga tataagcata ccgcaggtta cagacgataa aacatggtct tcacataact
      121 ttttaccgtc taacggcgta tgtccgcagc cgaaaacctt tcatgttttc ggcaggcaat
      181 atcgggcaag ctatgaaccg ttgtgcgtgt ttgccgaaaa aatccgtttt gccgtactgc
      241 tcgcctttat cattatgtcg gcttttgtc
//
LOCUS       DAXDXW010000214          268 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXDXW010000214 DAXDXW010000000
VERSION     DAXDXW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.215"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>268
                     /locus_tag="ACFB64_002213"
     CDS             <1..>268
                     /locus_tag="ACFB64_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210473.1"
                     /translation="DGSVKWFCGQPVKRNANAKDDAVTADKDKEIETKHLPSTCRDKS
                     SAGCTKTPEYYLNNGIWPKDNTSAGVASSPTDIKGKYVQSVTVA"
BASE COUNT           87 a           81 c           59 g           41 t
ORIGIN      
        1 aagacggttc ggtaaaatgg ttctgcggac agccggttaa gcgcaacgcc aacgccaaag
       61 acgacgccgt caccgccgac aaagacaaag aaatcgaaac caagcacctg ccgtcaacct
      121 gccgcgataa atcatctgcc ggttgcacaa aaacacccga atattacctg aataacggca
      181 tatggccgaa agacaacact tctgccggcg tggcatcctc ccccaccgac atcaaaggca
      241 aatatgttca aagcgttacg gtcgcaaa
//
LOCUS       DAXDXW010000215          265 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXDXW010000215 DAXDXW010000000
VERSION     DAXDXW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.216"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>265
                     /locus_tag="ACFB64_002214"
     CDS             <1..>265
                     /locus_tag="ACFB64_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050177927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFC1210474.1"
                     /translation="RGHRSVQQNNVEIAQAQAELARRQQEERKAAELLAKQRAEAERE
                     AQALAARRKAEAEEAKHQAAELAHRQEAKRKAAESAKRKAEEEE"
BASE COUNT           94 a           69 c           78 g           24 t
ORIGIN      
        1 cgcggacacc gttcggtaca gcaaaacaat gtggaaattg cccaagccca agcagaactt
       61 gcgcgccgac agcaggaaga acgcaaagcg gcagagttgt tggcaaaaca aagggcagaa
      121 gccgaacgtg aggctcaagc attggctgcg cgccgaaaag ccgaagccga agaggcaaaa
      181 caccaagctg ccgaacttgc acatcgccag gaagcaaaac gcaaagcggc agaatccgcg
      241 aaacgaaaag ctgaggaaga agagc
//
LOCUS       DAXDXW010000216          258 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXDXW010000216 DAXDXW010000000
VERSION     DAXDXW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.217"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..249
                     /locus_tag="ACFB64_002215"
     CDS             <1..249
                     /locus_tag="ACFB64_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC1210475.1"
                     /translation="IAVTTYPQNAASSVTTNAPIRKLPHHESRSISSLGFGAVAGVGI
                     DITPKLTLDAGYRYHYWGRLENTRFKTHEASLGMRYRF"
BASE COUNT           61 a           89 c           59 g           49 t
ORIGIN      
        1 attgctgtta ccacttaccc acagaatgct gcgtcaagtg ttaccacaaa tgctccgatc
       61 cgcaaacttc cccatcacga aagccgcagc atcagcagct tgggcttcgg cgcagtggca
      121 ggcgtaggca tcgacatcac gcccaagctg accctggacg ccggctaccg ctaccactat
      181 tggggacgcc tggaaaacac ccgcttcaaa acccacgaag cctcattggg catgcgctac
      241 cgcttctgat tccccgat
//
LOCUS       DAXDXW010000217          253 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXDXW010000217 DAXDXW010000000
VERSION     DAXDXW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.218"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..242
                     /locus_tag="ACFB64_002216"
     CDS             <1..242
                     /locus_tag="ACFB64_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC1210476.1"
                     /translation="GNGLVQSLSRKGNCLDNAAMESFFGTLKSECFHTCKYDSVTESE
                     AALHEYIRYYNNDRIKLKLKGLSPVQYRIQSLKAA"
BASE COUNT           78 a           55 c           59 g           61 t
ORIGIN      
        1 aaggcaacgg tctggttcag agtctgtccc gcaagggaaa ctgcttggac aatgcggcaa
       61 tggaaagttt cttcggaacg ttgaaatcgg aatgtttcca tacgtgcaaa tatgattccg
      121 ttaccgaatc ggaagcggca ctgcacgaat atatccgtta ctacaacaac gatagaatca
      181 agttgaaatt aaaaggactg agccctgttc agtacagaat tcagtccctg aaagccgctt
      241 gattaaactg tcc
//
LOCUS       DAXDXW010000218          248 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXDXW010000218 DAXDXW010000000
VERSION     DAXDXW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.219"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..215
                     /locus_tag="ACFB64_002217"
     CDS             <1..215
                     /locus_tag="ACFB64_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC1210477.1"
                     /translation="SSNVNKEIKDKKLSLWAKREDGSVKWFCGQPVKRDNAKADNDDV
                     KADADANNAIDTKHLPSTCRDTSSAT"
BASE COUNT           85 a           72 c           52 g           39 t
ORIGIN      
        1 cttcaagcaa cgtaaacaaa gaaatcaaag acaaaaaact ctccctgtgg gccaagcgtg
       61 aagacggttc ggtaaaatgg ttctgcggac agccggttaa gcgcgacaac gccaaagccg
      121 acaacgacga cgtcaaagcc gacgccgacg ccaacaacgc catcgacacc aagcacctgc
      181 cgtcaacctg ccgtgatacg tcatctgcca cctaaggcaa attaggcctt aaattttaaa
      241 taaatcaa
//
LOCUS       DAXDXW010000219          243 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXDXW010000219 DAXDXW010000000
VERSION     DAXDXW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.220"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
BASE COUNT           70 a           54 c           80 g           39 t
ORIGIN      
        1 atggcaacgg ctaaacctta aaaccgcccg aagctatatc tgccaagccg gggatagcac
       61 aaagcgatga agtatcgaac ttcaaagcgg ggcaacggca cggcgaaaca gtgaatgctg
      121 cgggcggttt tctcaaacgg aaaggacgaa ggcggtgaaa tattacggca cggcggctta
      181 cggcagcccc gattggggga tggaaagata ttacgcgcgg gaggatatgc ggcaggcttt
      241 gga
//
LOCUS       DAXDXW010000220          239 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXDXW010000220 DAXDXW010000000
VERSION     DAXDXW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.221"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            1..239
                     /locus_tag="ACFB64_002218"
                     /pseudo
     CDS             1..239
                     /locus_tag="ACFB64_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776296.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           49 a           68 c           70 g           52 t
ORIGIN      
        1 gcaatgggac atcggcggca gcggggcggt tttcccttcg ctcgcactgt ttctgctctg
       61 tttcatcata ggtatgcaca acacggggat gacgcttctg ccgggcggtg caatctgttc
      121 gacgcacatg gcccggcacg gcagccgact tgggcatcga aatcccgcgc gtgccgtact
      181 atagtggatt aacaaaaacc agtacggcgt tggcccgcct tagctcaaag agaacgatt
//
LOCUS       DAXDXW010000221          239 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXDXW010000221 DAXDXW010000000
VERSION     DAXDXW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.222"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
BASE COUNT           58 a           73 c           70 g           38 t
ORIGIN      
        1 ggctcaggga gcggcagggc aacccatcgg cacaaccgga cagttgccgg acaacacaac
       61 cgaatgcaag gcaggttgat gatgagtacc cgataccatt acgcaggtat agcggattaa
      121 caaaaaccgg tacggcgttg ccccgccccg gctcaaaggg aacggttccc taaagtgatg
      181 aagcaccggg cggatcggtt ccgtaccatt cgtactgcct gcggcccgcc gccttgtcc
//
LOCUS       DAXDXW010000222          232 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXDXW010000222 DAXDXW010000000
VERSION     DAXDXW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.223"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..137
                     /locus_tag="ACFB64_002219"
     CDS             <1..137
                     /locus_tag="ACFB64_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Imm41 family immunity protein"
                     /protein_id="HFC1210478.1"
                     /translation="IKASPWLPDSVGINERYERLKTMLRYIFTEKDIVNVQFDYYNKK
                     "
BASE COUNT           79 a           46 c           40 g           67 t
ORIGIN      
        1 aaattaaagc ttccccttgg ttacctgata gcgtgggaat taacgaacgt tatgaaaggc
       61 ttaaaacaat gctccgttat atttttaccg agaaagacat agtcaacgtg caatttgatt
      121 attacaacaa aaaatagcaa aacccggcaa tcaaaatgcc gtctgaaacc gtgtttaatt
      181 ttcaggcgac cttcccctta ttttaaacag gatattggga actaagttct tc
//
LOCUS       DAXDXW010000223          225 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXDXW010000223 DAXDXW010000000
VERSION     DAXDXW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.224"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>225
                     /locus_tag="ACFB64_002220"
     CDS             <1..>225
                     /locus_tag="ACFB64_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin C-terminal domain-containing protein"
                     /protein_id="HFC1210479.1"
                     /translation="VWVHNACPPRKTPSTPVYGNDSEAYAAAKKLGYRKIKERTRNDA
                     AIFKKGKSYISRDVDSHNGGAWKEASSPKNL"
BASE COUNT           88 a           36 c           50 g           51 t
ORIGIN      
        1 gtttgggttc ataatgcgtg tccgccaaga aaaacaccat ctacccccgt atatgggaat
       61 gattcagaag catatgcagc agcaaagaaa ttgggatata gaaaaataaa agaaagaacg
      121 agaaatgatg ctgctatttt taaaaaagga aagagttata tatcacgcga tgtagattca
      181 cataatggag gggcttggaa agaagcatct tcacccaaaa atctg
//
LOCUS       DAXDXW010000224          218 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXDXW010000224 DAXDXW010000000
VERSION     DAXDXW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.225"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
BASE COUNT           66 a           52 c           51 g           49 t
ORIGIN      
        1 cgatgaagca gtgcgacaac atagacaagg gcaaccttga ttcacactgc cataaccgct
       61 tgctgccaag gaaaacaaaa tgaatttgcc tattcaaaaa ttcatgatgc tgtttgcagc
      121 ggcaatatcg ttgctgcaaa tccccattag tcatgcgaac ggtttggatg cccgtttgcg
      181 cgatgatatg caggcaaaac actacgaacc gggtggca
//
LOCUS       DAXDXW010000225          216 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXDXW010000225 DAXDXW010000000
VERSION     DAXDXW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.226"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>216
                     /locus_tag="ACFB64_002221"
     CDS             <1..>216
                     /locus_tag="ACFB64_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210480.1"
                     /translation="KMNGKSVPIGCMWKNGKTVCPNGYVYDKPVNVRYLDEKKTDDFP
                     VTADGDAGGSGTFKEGKKPARNNRCFS"
BASE COUNT           68 a           56 c           57 g           35 t
ORIGIN      
        1 agaaaatgaa cggcaagtcc gtccccatag gctgcatgtg gaaaaacggc aaaaccgtct
       61 gcccgaacgg atatgtttac gacaaaccgg ttaatgtgcg ttacctggac gaaaagaaaa
      121 cagacgattt ccccgtcacg gcagacggtg atgcaggcgg cagcggaaca ttcaaagagg
      181 gtaaaaaacc cgcccgcaat aaccggtgct tctccg
//
LOCUS       DAXDXW010000226          216 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXDXW010000226 DAXDXW010000000
VERSION     DAXDXW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.227"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>216
                     /locus_tag="ACFB64_002222"
     CDS             <1..>216
                     /locus_tag="ACFB64_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210481.1"
                     /translation="TAGGKSVPIGCMEKGGKTVCPNGYVYDKPVNVRYLDETETDGFS
                     TTADGDAGGSGIDPAGRRPGKNNRCFS"
BASE COUNT           56 a           57 c           66 g           37 t
ORIGIN      
        1 agacagccgg cggcaagtcc gtccctatag gttgtatgga aaaaggcggt aaaaccgtct
       61 gcccgaacgg atatgtttac gacaagccgg ttaatgtgcg ttatctggat gaaacggaaa
      121 cagacggatt ttcaacgacg gcggacggcg atgcgggcgg cagcggtata gaccccgccg
      181 gcaggcgtcc cggcaaaaac aaccgctgct tctcca
//
LOCUS       DAXDXW010000227          216 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXDXW010000227 DAXDXW010000000
VERSION     DAXDXW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.228"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>216
                     /locus_tag="ACFB64_002223"
     CDS             <1..>216
                     /locus_tag="ACFB64_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC1210482.1"
                     /translation="LTPASAEAPHAQPPPEVSPAENPANNPDPDENPGTRPNPEPDPD
                     LNPDANPDTDGQPGTSPDSPAVPDRPNG"
BASE COUNT           53 a           93 c           51 g           19 t
ORIGIN      
        1 ctcacgcccg caagcgcgga agcacctcac gcacagccgc cgcccgaagt atcgcccgcg
       61 gaaaaccccg caaacaaccc ggaccccgat gagaaccccg gcacgcgtcc caatcccgaa
      121 cccgatcccg atttgaatcc cgatgcaaat cccgatacgg acggacagcc cggaacaagc
      181 cccgattccc cggccgttcc ggaccgccca aacggc
//
LOCUS       DAXDXW010000228          215 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXDXW010000228 DAXDXW010000000
VERSION     DAXDXW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.229"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
BASE COUNT           52 a           41 c           60 g           62 t
ORIGIN      
        1 tagaacaaca gcaatattca aagattatct gaaagtccgg gattctggat tcccgctttc
       61 gcgggaatga cggttcggtt gctacggtta ctgtcaggtt tcggttatgt tggaatttcg
      121 ggaaacttat gaatcgtcat tcccgcgcag gcgggaatct aggccggtcg gtgcggaaac
      181 ttatcgggaa aaaaggtttc ttgagatttt acgtc
//
LOCUS       DAXDXW010000229          215 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXDXW010000229 DAXDXW010000000
VERSION     DAXDXW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.230"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            <1..>215
                     /locus_tag="ACFB64_002224"
     CDS             <1..>215
                     /locus_tag="ACFB64_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFC1210483.1"
                     /translation="EAPAYGCIAVKPRRGGGHVFFVVGKDAEGRILGLGGNQGNMVSI
                     IPFDPADIDGYFWPSKLIGGKAVPSSP"
BASE COUNT           39 a           67 c           66 g           43 t
ORIGIN      
        1 tcgaagcccc cgcatacggc tgcatcgcgg tcaaaccgcg ccggggcggc ggacacgtgt
       61 tcttcgttgt cggcaaagac gcggaaggca gaatcttggg cttgggcggc aatcagggca
      121 atatggtatc catcatcccg tttgaccctg cggacattga cggctacttc tggccgtcca
      181 agctgattgg cggcaaagcc gtgccttcgt ccccc
//
LOCUS       DAXDXW010000230          210 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXDXW010000230 DAXDXW010000000
VERSION     DAXDXW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.231"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(<1..>210)
                     /locus_tag="ACFB64_002225"
     CDS             complement(<1..>210)
                     /locus_tag="ACFB64_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC1210484.1"
                     /translation="VCAVQTFDATAVGPILPITHERTGFEGIIGYETHFSGHGHEVHS
                     PFDNHDSKSTSDFSGSVDGGFTVYQL"
BASE COUNT           53 a           48 c           53 g           56 t
ORIGIN      
        1 agttggtaaa cggtaaaacc gccgtctacg ctgccgctga aatcagaagt gctttttgaa
       61 tcatgattat cgaacggact gtgtacttcg tgtccgtgtc ctgaaaaatg ggtttcataa
      121 ccgataatgc cttcaaaccc tgtccgttcg tgtgtaatag gcagtatggg gccgaccgca
      181 gttgcatcaa atgtttggac ggcgcaaacc
//
LOCUS       DAXDXW010000231          208 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXDXW010000231 DAXDXW010000000
VERSION     DAXDXW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.denovo.232"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
BASE COUNT           72 a           47 c           43 g           46 t
ORIGIN      
        1 atggagcgag aaaaacagca ccaacaggaa aaacaacaga gacatttaga gcgagagagt
       61 ggtttgagct tgtagctaac gccctacgcc tacggcttcg gttgttcaac ccttaaagaa
      121 ctcgcaacaa gttgcaaatt ctttaagggt tcgcaataaa aacaaccgct aaacatttct
      181 gcccagcggt tgaaaattta cctattca
//
LOCUS       DAXDXW010000232        22572 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG233
            SAMN08583400-rid23189053.guided.030, whole genome shotgun sequence.
ACCESSION   DAXDXW010000232 DAXDXW010000000
VERSION     DAXDXW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583400
            Sequence Read Archive: SRR6765497
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362639.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362639.1
            Genome Coverage       :: 209x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/17/2024 04:58:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,250
            CDSs (total)                      :: 2,204
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 105 of 166
            Pseudo Genes (incomplete)         :: 62 of 166
            Pseudo Genes (internal stop)      :: 44 of 166
            Pseudo Genes (multiple problems)  :: 44 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22572
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583400-rid23189053.guided.030"
                     /strain="NG233"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="30.16 N 120.10 E"
                     /collection_date="2012-04-08"
                     /collected_by="NCSTD"
     gene            complement(89..1297)
                     /locus_tag="ACFB64_002226"
     CDS             complement(89..1297)
                     /locus_tag="ACFB64_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFC1210485.1"
                     /translation="MIEYTPLSAEGKARILNGFMKPRLSRTQSVEQPKIVLVGAQPGA
                     GKSKAASLAKSELRQEGGYIHVDADIMRALIPAPEGVVYSSEQTQKDAGALAISVRNS
                     AKENRRNIVEEGTFRNAASISQFIRDRKSEGYGVEMLAVATASEESVAGIFKRYEEQH
                     AKGVSQPRFVEESYHNEAMAGFKDTLSQCESSFDRVRVTNRAGDILYDSLNRRQNQHE
                     TAKDALSAYQEITPKRLKQVVKAWDEIQLQAESRSIDPIPNYLGMVKQHSEAIYQRVE
                     EIYRQERVVANSEGATLQRKSGDTWQDIEKAEAKGMKAGIHMLGTAKPAKSGREYSGE
                     IVHKDEASVFQKTDQGLIRHKAVQGMAEGKFSSLSEQVEIGQKVSIKREGNELSVKPA
                     DASLKKTMKR"
     gene            complement(1294..1548)
                     /locus_tag="ACFB64_002227"
     CDS             complement(1294..1548)
                     /locus_tag="ACFB64_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210486.1"
                     /translation="MQMQETIPGINYEVALELLGDSMRPLIQARESEFVKDKPNQAFI
                     EYCTARLEAIDMLRDSLSPEDTDIIARIFNPDDQTVGRVK"
     gene            complement(1925..2446)
                     /locus_tag="ACFB64_002228"
     CDS             complement(1925..2446)
                     /locus_tag="ACFB64_002228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210487.1"
                     /translation="MNMKTVQEYEIIIRLTKENGRYGAAAETVSGGLLESVPPLSAAG
                     NDALYALLHALALKNNFSGCLKEAQRRESVKNIEAKIEEKMKPIELQYYFSDMNVGEF
                     NAYLTVKNLAKPTFISHIDIDEDDKMFIANLAKGIASEYKSLFYLDVNRQTIEKALLR
                     YLEVCVSCNSAQD"
     gene            2834..2950
                     /locus_tag="ACFB64_002229"
     CDS             2834..2950
                     /locus_tag="ACFB64_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HFC1210488.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            2966..4885
                     /gene="tet(M)"
                     /locus_tag="ACFB64_002230"
     CDS             2966..4885
                     /gene="tet(M)"
                     /locus_tag="ACFB64_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFC1210489.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDPVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQKYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            4977..5450
                     /locus_tag="ACFB64_002231"
     CDS             4977..5450
                     /locus_tag="ACFB64_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210490.1"
                     /translation="MPHEQMIDAFSAVIQNSLSLYEEQTPIYYYLTICGSWVLTALGI
                     LSLVSVFFTIKQKKPYRFIVPILFLWLGYESIHMPRPNLAVFYGYNLAQLSYPANENP
                     NSFLHLADGSNPICCFIGEDEYLQAKRLGFKEYADAKAKALVDLVEMEKKSKQSD"
     gene            complement(5602..6498)
                     /locus_tag="ACFB64_002232"
     CDS             complement(5602..6498)
                     /locus_tag="ACFB64_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine modification methylase"
                     /protein_id="HFC1210491.1"
                     /translation="MFTHSVCSYPQRGNYGKSGYRGNCTGHIIRDFVESYLAPEGLFA
                     DPSIGSGTSSDVAEEMGIRFKGTDLHQGYNLLTQDFAAFLGEQANLIWWHPPYWNMIR
                     YSGNQWGDAANPWDLSQMDLEQFQEAMLLAMMNIHDATEQGGHYGILMGNMRKDGRYY
                     NLSSMVERMAAGRLVDEIIKIQHNCVSDSRQYGGKLVRIAHEKLLVFKKEIQTALYFL
                     AKAMNRVEAVTAVTWKAAVRRVLQAADGRNMGLTEIYGEMEPYAAARAENRNWQAKVR
                     QVLQDERFFLRVGKGIYALKTE"
     gene            complement(6502..6843)
                     /locus_tag="ACFB64_002233"
     CDS             complement(6502..6843)
                     /locus_tag="ACFB64_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210492.1"
                     /translation="MLYWDDVSDSFDGWMNLLRQLGGDSFINFEQDIWETARTYETVP
                     HFGNLRQHHLLERLQDTIRNRWPFLRTGFLINALDTHFYVNDEPVETMRDLDAVLGCH
                     YRENEDDLKEE"
     gene            complement(6857..7171)
                     /locus_tag="ACFB64_002234"
     CDS             complement(6857..7171)
                     /locus_tag="ACFB64_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210493.1"
                     /translation="MASYTNRLTGHPNVFVEQNIWSNGELMGFSPINVMWDGRNAPTL
                     LCRYTFDGGQYYSLQVSEAAELETRGYQIVCDDLQCLKLKTAKARRSGILALILADAG
                     IE"
     gene            complement(7538..7897)
                     /locus_tag="ACFB64_002235"
     CDS             complement(7538..7897)
                     /locus_tag="ACFB64_002235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210494.1"
                     /translation="MSLSAENRTFYLVETGEIGFGSSALFISQSLEEAEAVVADFFKG
                     DEKLHYETDGNQPEISLGMGEPQEEEYIPFLPREPFFWTKGGFGMMGTEYVTIRKTIN
                     GYLEGAGSFRLIKSQQD"
     gene            complement(7894..8328)
                     /locus_tag="ACFB64_002236"
     CDS             complement(7894..8328)
                     /locus_tag="ACFB64_002236"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HFC1210495.1"
                     /translation="MSIYRPASRERKNIKFFADGQYIQEGYKALCWMMRDVVDNHQMH
                     AININLINLLFAQQQYLRDLGRPNPELVLHSGFRTRRHNDSLEGAAKNSQHLSGNAGD
                     FHIERASLSELAALARRFRVGGIGIYPTFIHNDIGVYREWRK"
     gene            complement(8401..8454)
                     /locus_tag="ACFB64_002237"
                     /pseudo
     CDS             complement(8401..8454)
                     /locus_tag="ACFB64_002237"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
     gene            complement(8451..8921)
                     /locus_tag="ACFB64_002238"
     CDS             complement(8451..8921)
                     /locus_tag="ACFB64_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor"
                     /protein_id="HFC1210496.1"
                     /translation="MAFFLTYHKQAREGWFWRVFGMAFDGRLRQTLQNIAVDYRKTSQ
                     NQPLAFDDYLEMLQRHEEEKMGVSAERNQSEQAAVKAFQKQYGLNHRVDTHADTKTLS
                     ITITATDEDFIPAVEMGLEQVLGSLAAGETANEGGNEYGRYAFMVSGNVKGRER"
     gene            complement(8954..11953)
                     /locus_tag="ACFB64_002239"
     CDS             complement(8954..11953)
                     /locus_tag="ACFB64_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /protein_id="HFC1210497.1"
                     /translation="MQNKKPFYEQVAEKLIEQLRQGTAPWQKPWDGGGLPDFMPHNAI
                     TGRRYRGINNLWLQAQGRKDPRWITYKQASSIGAQVRRGEKGTVVEYWQFTEEVPKTD
                     EKGQEIIGQDGKPEKETVRLERPKVFHAVVFNGEQIDGLPPLKLRPLSWNPDERAENI
                     LKNSGAVIDHRHGDRAFYSPSRDSITLPLREQFESPGAYYETALHELGHWTGHADRLN
                     RDLSHPFGSEGYAREELRAEIASLMLSQELGVSFNPGQHASYVASWIMVLQDDPMEIM
                     RAASDAEKIQGYVMAFDQVREIEQQNEAAVSISDRQNIDEASLAEEKNLINAVDKNDV
                     AAIINFMAEEKMHEGLKRFSLVISEMDDATFQNAFWQRHQKPENGESFMENLNAAKEA
                     LDEAIEQSSYRTVEEQIRQFLSEQDKESVRVVSDERVYVQIPYADREQAKMLGAKWDK
                     EEKSWYIPPGVNQEPFRKWLDSATNQAKTVTDNTPAIAKTLIDVPFREKNEARALGAK
                     WDKDQKSWYIPEGVNTALFARWMQPKQEALAEEAVPDDTPQEEAVECQVAAIQQEYQA
                     AKTEEAPAEENASRVYLAVPYEEKEEAKALGAKWDKANKSWFMESSNANLPAASKWLP
                     EKQQTQSMPAMSPVEEFADKLREMGCVVANGHPFMDGQKHRIETVGDKKGERSGFYVG
                     HLDGHPAGYIKNNRTGAEVKWKAKGYSLSPEEKAKLAAEAAQKLQMRSEQKKQAQEAA
                     VGRIKYYMESYLPVQTGTPYLNAKGIKPQPGIFTDREGQKTFIPVYNAAGELRSMQYI
                     QADGTKRFAKDSEQEGCMHVVGQQDLAKAKTIILSEGYATAASIKEATDDTVASVAAF
                     NSGNLPLVAKVLSAKYPQAQFLVAGDDDLAVEAKQGNNPGKEKALEAAKILNCRAVFP
                     VFAPGEQSSEHKAFTDFNDLAQKSKFGREGLAKQINEAIHARPANELQTLKTRGLQEE
                     ASQDRPVEQTKRQQRATTRKTASRGSR"
     gene            complement(11956..12120)
                     /locus_tag="ACFB64_002240"
     CDS             complement(11956..12120)
                     /locus_tag="ACFB64_002240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210498.1"
                     /translation="MIAEKVADLNNPGMEVDFDPAEAEALGAFEETAMDAETARDSVA
                     DLSREVAEGA"
     gene            complement(12142..14337)
                     /locus_tag="ACFB64_002241"
     CDS             complement(12142..14337)
                     /locus_tag="ACFB64_002241"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFC1210499.1"
                     /translation="MSRLYIAEKPEVARAIAAALGVGIERRNGYFRCSNGDNITWCYG
                     HMLALCDPEDYDSKYGNWKFEDLPIAHIPWRKKPLSDKKEQIGVIRDLLAECTEIVHA
                     GDPDDEGQLLVDELLEFLNCAKPVKRVLINDNNERSVKRALSAIEDNAKFRGLSQSAE
                     ARSVADQLYGYNMTRAYTLKAAEQGYQGVLSVGRVQTPILGLVVRRDRENQSHVKTHY
                     YHVYADLDVDGVRFTARYVPNKDQDPIDDKGRLSDPLFARQIAEAASGQPVKILAAKT
                     SAKRQSPPLPYNLLKLQADCSRKFGLKPDQVKDIGQSLKDKHRLITYHRSDCQYLNDE
                     HFDDAADVLDAVGKTASVFKNAIASADTKIKSRAFNPDKVSAHHAIIPTVKTANMDDL
                     TEDEKRVYLLIARAYIAQFWPDHEYNQTDIEAEIAGKKFVCRSNVTVKPGWMALYKND
                     QGNAETATDPEDLSVDLQMLKPGNSGRCLTAEETQFETKPRPLYTMDTLLNDLTRVAK
                     YVQNPKLRELLIEKDKGKEGEHGGIGTPATRDEILRNLLDKGFLLEKGKNIVSSEIGQ
                     QLYDALPDQAKFPDMTALWHEQQQHIVQGGLDAQRFVNSLMEYISGEVERVGREGISL
                     NVKKYPCPECGKPLRRMRGSKGFFWGCTGYKDGCKVSFSDKAGRPVLAPKKAEPSSKF
                     KCMACTHPLVRRPGKQKGTHFWGCSNYPECKQYYPDAGGKPDYSKHTKG"
     gene            complement(14340..14876)
                     /gene="traF"
                     /locus_tag="ACFB64_002242"
     CDS             complement(14340..14876)
                     /gene="traF"
                     /locus_tag="ACFB64_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer signal peptidase TraF"
                     /protein_id="HFC1210500.1"
                     /translation="MRRLFDRAMLAMCLTAALLVTGYGLLYAAGFRINDTRSVAKGVY
                     RISGEAVRRGSYITFCPPDTEVFRMARRRGYIRAGFCPENYSGLMKFVAGIPGDNYRF
                     TDEGLLINGRLLGGTRPLWQDGLGQPLPVFEGEGRLKDDEYILIGNAVANSFDARYYG
                     IVRREHIRDVVKPIWIEE"
     gene            complement(14873..16762)
                     /locus_tag="ACFB64_002243"
     CDS             complement(14873..16762)
                     /locus_tag="ACFB64_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFC1210501.1"
                     /translation="MFRSKNKRNNAVGHQNQAQATGSGRLVNFICLVLVLLAGMGAAT
                     QYFAHQFQYHSQLGYGIGKFYMPWSILIWYGKWHGRYEQQFMTAFSIMLMVVAVGMIG
                     LLVWNIVRKNTNKAHESLHGSARWADRKDIEAAGLLAKKGEDSDGVYVGGWVDKKGTL
                     HYLRHSGPEHVLTYAPTRSGKGLGLVLPTLLSWPHSTFITDLKGELWALTAGWRKKYA
                     HNKVIRFEPASAQGSAAWNPLDEIRVGTEYEIGDVQNLATMIVDPDGKGLNSHWDKTA
                     FALLTGVILHALYKARDDGGVATLPSVDRMLSDPEKPVGDLWVEMATYPHADGRPLPV
                     ISSAGQDMLDRPEEEAGSVLSTAKSFLALYRDNVVAANVSRSDFKIKDLMNDEDPVSL
                     YIITQPNDKARLRPLVRILINMIIRLSADKMDFENGRPKVHYKHKMLAMLDEFPSLGK
                     LEIMQESLAFIAGYGIKCYLICQDLNQLKSRETGYGPDETITSSCHVQNAYPPNRIET
                     AEHLSKLTGITTVVKEQITTSGKRVSAMLGQVSRTTQEVQRPLLTADECMRMPGPVKD
                     ARGLITQAGDMVIYVAGYPAIYGRQTLYFKDPVFEARSKVPPPCHTDRTQKKTANTPS
                     AFDTL"
     gene            complement(16779..19172)
                     /locus_tag="ACFB64_002244"
     CDS             complement(16779..19172)
                     /locus_tag="ACFB64_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFC1210502.1"
                     /translation="MIAKKIPMRKANLSSFEGLVTYLAGSQGVANRVGKVEIHNYESS
                     DLRWAVQETLAVQDQNKRSAADKTYHLMVSLAGGEFLTDEQLSRIEQSMIEALGYAEH
                     QRISVVHRDTDNLHFHVAINKVHPEKKTIHEPYNDFKILGRECRKFEEEYGLQRVQRG
                     GRGNKKIDMEEVAGIESLIGWIRRECGEQIKEAKTWEQLYHVLSDHGLEIKLRGNGFV
                     ISDGEHHVKASSVDREFSKSALEKRLGAFDQKTGVKAKPSKTYREKPLSKANVDDLFR
                     QYKEEKELQKSRRQDFFLHLAQNRDMEIGRIKEKYAFRRSLIRHSSSGIARRILYRML
                     FRQMKDEIRSVRTDYARRRAVFFEQNKGFAWADWLKHRAAEGDVRALDALRRRGKDAG
                     YKGNRFVGKESGGFAGIKVDTVTKKGTVIYAEDGMTIRDTGSVLKLAAIPDDKAVSRA
                     LEIAIGRYGKVLTIGGTRHFQDKVARIAAAKGLDVSFTDGRLNHLYQQYVKEYEYVRE
                     RTDRYRQADGTGRADGRAGQQRGGAGYAGGKPRRYRWHGYDASRNRGFHAGISGYPIP
                     KLSDSGGIGAGRGQSASAGSDSLRGMPERSLVRIQGRTQVLLPRDVSSHLEQQGSANY
                     QAVRRTIHRTGGVAEIRQMQAVVGLVAKPGKKPPGFRRGSLRGMEGLAEIKISSAAVV
                     EIPKNPEPAQENERLTHSLAPIDKYIAERQEKIDKKFDILNHRKYNPSDSGVMLFRGI
                     RNIDGQALALFQPKEVEEILVMSVTPYVSRRLSSVKLGSEVKVTPTGQIRPVKGRHR"
     gene            complement(19175..19552)
                     /locus_tag="ACFB64_002245"
     CDS             complement(19175..19552)
                     /locus_tag="ACFB64_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HFC1210503.1"
                     /translation="MAGARKRPKYISVYVSEEEWKTIESLAKSVGLSQSAYLKLLGLG
                     YKPASVYDGEAVKALAKINADQGRLGGLIKMWLANDERLMMFDHDQLAKAIFGVLEQI
                     GEMQESMLEIVRSRRILAAKKIG"
     gene            19602..20303
                     /locus_tag="ACFB64_002246"
     CDS             19602..20303
                     /locus_tag="ACFB64_002246"
                     /inference="COORDINATES: protein motif:HMM:NF036299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK family protein"
                     /protein_id="HFC1210504.1"
                     /translation="MASLAEQDNPLSLPANKDCDVHVHHISCPNSGVQYAKDYTPNNS
                     IYRTYFDDKPPKNALSQTKNDLLYRIIVFKTILLGSFGKALSKNWQSPIEMIQMGKIE
                     KLVLAELAAMQKKGSSAGNKARFLAMQEDIKILLVDRNIPVISVWRILSEKKYVPYSY
                     ETFNRYCKMYIFKSDTTAADQTDTAQSTQEKPDSIRTDKEPPSPASEPIIAGGNTKSS
                     SGFTFNNNPDGKDLF"
     gene            20304..21029
                     /locus_tag="ACFB64_002247"
     CDS             20304..21029
                     /locus_tag="ACFB64_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HFC1210505.1"
                     /translation="MAIIHISLQAKGGVGKSFLALMLSMFYRHIERPVKIFDTDPANA
                     TLFGFPALEAEKFEIMKKDDIDKRLFDRLFSQILSATDDVIIDVGANAFIPLCSYIIS
                     NNLITMLTQEGHQVIIHTSIVGGQNLKHTVNGFDALCSQFPESTGFVIWLNPFWGEIE
                     FEGKSFEKLKVYEKHKSRIRSIIKIPEWDASLHGRDLAAMLEKQKTFDEALASEDFSI
                     FERQRLNIIKNEMFQTIETAQIV"
     gene            21037..21519
                     /locus_tag="ACFB64_002248"
     CDS             21037..21519
                     /locus_tag="ACFB64_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC1210506.1"
                     /translation="MDEKVKAILATIVEKNGIALSPKDPIMVVFTALEYMLSEQHAEQ
                     KALTVLFKAQVEEATGNWIQSVHQQTEELVSQQLNTTKEQAKEMSKLLLHETDRLLEQ
                     RITDAVKDFEGKLYGINDNAMNAVELRIKETRNTAILNLTAALIVLLAVIILAFKFTF
                     "
     gene            complement(21576..22112)
                     /locus_tag="ACFB64_002249"
     CDS             complement(21576..22112)
                     /locus_tag="ACFB64_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncP plasmid survival protein KfrC family
                     protein"
                     /protein_id="HFC1210507.1"
                     /translation="MNEDKGLVAKAVAAETEQEELFESRQVIEETFAGVLNTYVLEKT
                     EQVERIEEKLEQLISGQMAKLQALQNARPGMLSMPSKKQTWSANIAVAQSRLNRLHDR
                     LEDVREIRDGMALGVPKIQELAARKLRMEEPDLAKKFDDVQTAVRVHNLHEKQRKEKE
                     QQKRQAGLSLSLGQKIVR"
     gene            complement(22149..>22572)
                     /locus_tag="ACFB64_002250"
     CDS             complement(22149..>22572)
                     /locus_tag="ACFB64_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="KfrB domain-containing protein"
                     /protein_id="HFC1210508.1"
                     /translation="GGGILKTVLLELFTSFLKIFSRMEAEKQRVLVLNKQHLFETEKD
                     GKWITQAVKPAPANMKPGIYLLHTAKSPQDSVRYEGQIVFQNDSYVYQKTRQSIVKYP
                     CSLFSSVPSIGEVVSISVKDKAATVEKAGIGEKKTLTR"
BASE COUNT         5475 a         6039 c         4804 g         6254 t
ORIGIN      
        1 aagcctcttt tatcattctg atagcattac tttcttgggg ctcaacttta ttcataataa
       61 aatcctaatc ttaattttat attactcatc atcgcttcat cgttttcttt aatgaggcat
      121 ctgccggttt aacggacaat tcatttcctt cccgcttaat agaaaccttc tgaccaattt
      181 ccacctgttc ggacaaacta gaaaactttc cttccgccat accctgaacc gccttatggc
      241 gtatcagacc ttggtcagtc ttctggaaaa ccgatgcctc gtctttatgg acaatctctc
      301 cgctgtattc cctgccggat ttagccggtt tcgccgtgcc aagcatgtga ataccggctt
      361 tcatcccctt ggcttcagcc ttctcaatat cctgccatgt gtcgcctgat ttgcgttgca
      421 gtgttgcccc ttctgaatta gccaccaccc tttcctgacg gtaaatctct tccacacgct
      481 gataaattgc ttcactatgc tgtttgacca tgccgagata attaggtatt ggatcgatac
      541 tgcggctttc tgcctgaagc tgtatctcgt cccatgcttt tacaacctgt ttcaaccgtt
      601 taggggttat ttcctgataa gccgatagtg catcttttgc cgtttcatgc tggttttgtc
      661 gccgattcaa actgtcataa aggatgtctc ctgcgcggtt ggtaacacga accctgtcaa
      721 aacttgattc acattgagac aacgtatcct tgaatccagc cattgcctcg ttatggtacg
      781 actcttcaac aaatctaggc tgtgaaacac ctttggcatg ttgttcctca taacgcttaa
      841 aaatcccggc tacactctct tcagaagcag tggctaccgc cagcatttct accccgtacc
      901 cttctgattt tctatcacgg ataaattggc tgatacttgc agcatttcta aatgtcccct
      961 cctccacaat attccgccgg ttttcttttg cactattccg aacactaatg gccaaagcac
     1021 cggcatcttt ctgagtttgt tcagacgaat acaccacccc ctccggcgca ggaatcagtg
     1081 cacgcataat atctgcatcc acatggatat atccgccttc ttggcgtaac tcgcttttag
     1141 ctaaagaagc tgccttactc ttacccgctc cgggttgtgc cccaactaga acaattttgg
     1201 gttgctccac actttgcgtt ctagataaac gtggcttcat gaaaccattc agaattcttg
     1261 ccttaccttc ggcacttaaa ggggtgtact caatcatttt acacgtccaa ctgtttggtc
     1321 atccggattg aaaatacgcg cgataatatc tgtgtcctcc ggagagagac tgtcacgcaa
     1381 catatcgata gcctcaagcc ttgctgtaca gtattcgata aacgcttggt ttggtttatc
     1441 ctttacaaac tcggactctc ttgcctgaat taatgggcgc atcgaatctc ccaacaactc
     1501 caaagccact tcataattga taccaggaat agtttcttgc atttgcatct tggcttcctc
     1561 caaaagttat ccacaaattt tgttagtaag ctgcccatat cgccagcctg ccacatataa
     1621 taaattgaat cttcgtcatc aaaccgtttt tgcctgcttt ttaagcaaca tggttcaaaa
     1681 cctatgtttg aataataagc cagttcaaaa taaataacaa cgtttttgac ctatacttcc
     1741 agaccttaac gaaacatggt tcataaagta cactttttgc acctacttat ctaagttacc
     1801 cacctcttaa tcaagatttg gccattgccc accatatccc ataatgtgta gaaaccagta
     1861 gcgttgggtg gatgacggcg tttcgggttt tccgcaggcg gcaggcagcc tgcaatggta
     1921 gtggctaatc ctgcgcgcta ttgcatgaga cacaaacttc aagatagcgc agcaaggctt
     1981 tttctatcgt ttgtcgatta acatcaaggt agaagagaga cttatattcg gatgcaatgc
     2041 cttttgctag gttggcaata aacatcttgt cgtcttcatc tatatcaata tggctgataa
     2101 aagtcggttt ggccaaattc ttgacagtaa gataggcatt gaattctcca acattcatat
     2161 ccgagaaata gtattgcagc tctatcggtt tcattttttc ttcgattttg gcttcaatat
     2221 tcttcaccga ttcccgtcgt tgtgcttctt tcaggcagcc tgaaaagttg tttttcagtg
     2281 ccaatgcgtg cagcagagcg tacagggcat cgttaccggc tgccgacagc ggaggcacgg
     2341 attcgagcag tccgccggag acggtttcgg ctgccgcacc gtagcggccg ttctctttcg
     2401 tcaggcggat aatgatttcg tattcttgga cggttttcat gttcatactc ccagttgtct
     2461 ttgttcagtc cgtgctaacg gcgtatccag ccgcactccg gcggcggcat cctgcccgac
     2521 tctctttcac gtttataatt ataggataat ttatcctact tgtcaaataa attttagccg
     2581 ctaaattttt actctcgctt atttattcat tataataaat taatcgttat tttgtttaac
     2641 tgtaaaattg gagattcctt gacaaatatg ctcttacgtg ctattattta agtgactatt
     2701 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagcg
     2761 ggaacaaata attagatgtc cttttttagg agggcttagt tttttgtacc cagtttaaga
     2821 atacctttat catgtgattc taaagtatcc agagaatatc tgtatgcttt gtatacctat
     2881 ggttatgcat aaaaatccca gtgataaaag tatttatcac tgggattttt atgccctttt
     2941 gggtttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     3001 tgatgcagga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     3061 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     3121 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     3181 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     3241 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     3301 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     3361 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     3421 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     3481 tgaacaatgg gatatggtaa tagaaggaaa tgattacctt ttggagaaat atacgtctgg
     3541 gaaattattg gaagcattag aactcgaaca agaggaaagc ataagatttc ataattgttc
     3601 cctgttccct gtttatcacg gaagtgcaaa aaacaatata gggattgata accttataga
     3661 agtgattacg aataaatttt attcatcaac acatcgaggt cagtctgaac tttgcggaaa
     3721 agttttcaaa attgagtatt cggaaaaaag acagcgtctt gcatatatac gtctttatag
     3781 tggcgtactg catttgcgag atccggttag aatatcggaa aaggaaaaaa taaaaattac
     3841 agaaatgtat acttcaataa atggtgaatt atgtaaaatc gataaggctt attccgggga
     3901 aattgttatt ttgcagaatg agtttttgaa gttaaatagt gttcttggag atacaaagct
     3961 attgccacag agagagagaa ttgaaaatcc cctccctctg ctgcaaacga ctgttgaacc
     4021 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     4081 cccgcttctg cgatattatg tggattctgc gacacatgaa atcatacttt ctttcttagg
     4141 gaaagtacaa atggaagtga cttgtgctct gctgcaagaa aagtatcatg tggagataga
     4201 aataaaagag cctacagtca tttatatgga aagaccgtta aaaaaagcag agtataccat
     4261 tcacatcgaa gttccaccga atcctttctg ggcttccatt ggtctatctg tagcaccgct
     4321 tccattaggg agcggagtac agtatgagag ctcggtttct cttggatact taaatcaatc
     4381 gtttcaaaat gcagttatgg aggggatacg ctatggctgt gaacaaggat tgtatggttg
     4441 gaatgtgacg gactgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     4501 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     4561 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     4621 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     4681 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaaaaat atcgtagtga
     4741 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     4801 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     4861 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     4921 ttaaataaga tgaaatattt tttaataaaa attaactgtg agtgattgga gacaccatgc
     4981 cacatgagca aatgattgat gctttttcag cagtcattca aaactcctta agcctatatg
     5041 aagagcaaac tccgatatac tactacctga ctatctgcgg ttcttgggtt ctgactgcac
     5101 tcggcatcct ctccttggtg tccgtattct ttaccatcaa gcagaaaaaa ccataccgtt
     5161 ttatcgtccc gattctgttt ttatggctgg gatatgaaag catccacatg ccgcgcccga
     5221 atcttgctgt attctacgga tacaacttgg cgcagctttc ctatccggca aacgaaaatc
     5281 ccaactcgtt cctacatctc gcagacggct caaatccgat ttgctgcttc ataggcgaag
     5341 acgagtattt gcaagccaaa cggttaggtt tcaaagagta tgccgatgca aaagcaaagg
     5401 ctttggttga tttggttgaa atggagaaaa aatccaaaca atctgattga atcttaaatt
     5461 caataataac gcggtagggg gctggtcaag cgaaggttca ggctctgact gaaatttcgc
     5521 ttggacaggg ggtgtggggc ggagcgcagc gaagggctac cgcgtggcgg ccgaagccgc
     5581 ccgacaaact ccagtttgtc gctattccgt tttcagtgcg taaatacctt tccccactct
     5641 cagaaaaaac cgctcgtcct gtaatacctg cctgactttg gcctgccagt tcctgttttc
     5701 tgcccgcgca gcggcatacg gttccatttc gccataaatt tccgtcaagc ccatattcct
     5761 cccgtctgcc gcctgcaata cccgtcttac ggcggctttc catgtaacgg ccgttaccgc
     5821 ctcaacccta ttcatcgctt tagccaaaaa gtacaaggcc gtctgaatct cttttttaaa
     5881 gaccagcagt ttctcatggg caatccgtac cagctttccg ccatattggc ggctgtcgct
     5941 gacgcagttg tgctggattt tgataatctc atccaccagc cgccccgccg ccatccgttc
     6001 aaccatgctg ctcaaattgt agtaacggcc gtccttcctc atgttgccca ttagaatccc
     6061 ataatgcccg ccctgttcgg tcgcatcatg gatgttcatc atggccagca gcatagcctc
     6121 ttgaaactgt tccaagtcca tttggctcaa atcccacgga ttggctgcat caccccattg
     6181 gttgccactg tatcgaatca tgttccaata aggcggatgc caccaaatta aattggcctg
     6241 ttctcctaaa aatgccgcaa aatcctgagt cagcaggtta tatccctgat gcaaatccgt
     6301 tcctttaaaa cgaatgccca tttcctccgc aacatccgaa ctggtaccgc tgccgatgct
     6361 cgggtcggca aacagtcctt ccggagcaag atagctttcc acaaagtcgc ggatgatatg
     6421 ccccgtgcaa tttccgcgat aaccgctttt accgtaattt ccgcgctgcg gataagaaca
     6481 aacagaatgc gtaaacatgg ttcattcctc ctttaaatcg tcttcatttt cgcgataatg
     6541 gcaacctaat actgcatcca aatcccgcat ggtttccacc ggctcgtcat tgacataaaa
     6601 gtgtgtatcc agcgcattga ttaaaaaacc cgtccgcaag aacggccacc ggttgcggat
     6661 ggtatcctgc aaacgctcca gcaaatgatg ctgccgaagg tttccaaaat gcggaacggt
     6721 ttcatatgtc cgtgccgttt cccaaatgtc ctgctcgaag ttgataaacg agtcgccgcc
     6781 gagttggcgc agcagattca tccaaccatc gaaactgtcg gacacatcat cccaataaag
     6841 cataatttcc tccttatcac tcaatccctg catcggccaa aatcaaagcc aagatgccgc
     6901 tgcgccgtgc tttggcagtt ttcagtttca gacactgcaa atcatcgcaa acaatctgat
     6961 acccgcgcgt ctccagttcg gccgcttctg acacttgcag gctgtaatac tgcccgccat
     7021 cgaatgtgta tcggcaaagc agtgtagggg catttcgtcc gtcccacata acgttaatcg
     7081 gactgaatcc catcaactcc ccattgctcc aaatattctg ctcgacaaat acattcggat
     7141 gcccagttaa tctgttggta tagcttgcca tctctgtctc ccgtactttc cttggtgtta
     7201 aacggttttt gaactgaacc agcatcggcg tttccgcccc gctgtgatga cgttaaaaga
     7261 caaactgctt tgagatacaa ggctgccgca ggcaccgcag gctcggcctt gtatctcaaa
     7321 gcagtttgtc ttaggaatca cgcaacagcg gggcgggaat gccggttgcg tcgaacggcc
     7381 aaaagccgtt tgacaccaag gaaaaccttc tgccggccgc agccggctca ttgcccctca
     7441 cggggaaggg gtggggttcg gagaggcggt gccgcagcac ggccgatact taaaccaaga
     7501 gcggtttgat gccttagcat gaaaccgctc tttccgttca atcctgctga ctcttgatta
     7561 gcctgaaact cccagcacct tcaagataac cgtttatagt cttgcgaatg gtaacatatt
     7621 ccgtccccat cattccgaat ccgcccttag tccaaaaaaa cggctccctc ggcaaaaaag
     7681 gaatatattc ctcctcttga ggctcgccca tgccaagact gatctcgggc tgattcccat
     7741 ccgtctcata atgcagcttt tcgtcccctt tgaaaaaatc ggcgacgacc gcttccgcct
     7801 cttcaagaga ttggctgata aataaagccg aactaccaaa gccgatctca cctgtctcta
     7861 caaggtagaa agttcgattt tcagcggaca gactcatttt ctccactccc gataaacgcc
     7921 tatgtcgtta tggataaagg ttggatagat accgatccca cctaccctga accttcttgc
     7981 caaagcggca agctcggaaa gggacgctct ttcgatatga aagtcccccg cattgccgga
     8041 caaatgttgg gagtttttgg ccgccccctc taacgagtcg ttatgtcggc gcgtgcggaa
     8101 tccgctatgc aaaaccaact ccggattcgg tctgcccaaa tcacgcaaat actgctgctg
     8161 ggcaaacagc agattgatca aattgatgtt gatggcgtgc atctgatggt tgtccaccac
     8221 atcccgcatc atccaacaca gagccttata gccctcttgg atatactggc cgtcggcaaa
     8281 gaatttgatg tttttccgtt cccgggaagc aggccggtaa atggacaata cccggtttcg
     8341 tttccaaaac cggctgtcat ccgccaaaac gatttccggc aacagtaaaa ggccgcctga
     8401 tgaagcaagc gcggccgctt tggccgacag ctttaaaaaa tcccgacgat tcaacgctcc
     8461 ctccctttca cgttaccgct gaccataaaa gcataccggc cgtattcgtt acctccctca
     8521 ttcgccgttt ctccggccgc aagggatccc aaaacctgct ctaatcccat ttcgaccgca
     8581 ggtataaaat cttcatctgt cgccgtaatg gtaatgctga gcgtcttggt atcggcatgc
     8641 gtatctaccc gatgattcag tccgtactgc ttctgaaatg ccttgacggc ggcctgttcg
     8701 gactgattcc gttcggccga cacgcccatt ttttcctctt cgtgccgctg caacatttcc
     8761 aaataatcgt caaacgccaa gggctggttc tggctggttt tccggtaatc gacggcgata
     8821 ttttgcagcg tctgccgcag gcggccgtca aaggccatgc cgaaaacccg ccagaaccat
     8881 ccttccctgg cctgtttgtg ataagtcaga aaaaaagcca ctctgtataa tttgtcctta
     8941 tccataacac cccctatcgg ctgccgcgcg atgcggtttt ccgggttgtt gcccgctgct
     9001 gccgcttggt ctgttcgaca ggccgatcct gagacgcttc ttcctgcaaa ccgcgtgttt
     9061 tcagggtttg caattcgttt gccggtcggg catgtatcgc ttcgttgatt tgcttggcca
     9121 aaccttcgcg gccgaacttg gatttctgag ccaaatcgtt gaaatcggta aatgccttat
     9181 gctccgacga ctgctctccc ggtgcaaaca ctggaaacac tgcacggcag ttaaggattt
     9241 ttgccgcctc caacgctttt tctttgccgg gattgttgcc ctgcttggct tcgacagcca
     9301 aatcatcgtc tccggcgacc aaaaactgag cctgagggta tttcgcagac aagactttgg
     9361 ccaccaaagg caggttgccg gaattgaacg cggccacact ggccaccgta tcgtcggttg
     9421 cttctttaat cgaggcggcc gtagcatagc cttccgataa aataatggtt ttggccttag
     9481 ccagatcctg ttggccgaca acgtgcatac aaccctcctg ctcgctgtct ttggcaaaac
     9541 gtttggtacc gtccgcctga atgtattgca tggagcgtaa ctccccggct gcgttgtaca
     9601 cgggaatgaa agttttctgc ccctcgcggt cggtaaagat acccggctgc ggcttgatac
     9661 ccttggcatt cagataaggc gtgcctgttt gaacgggcag gtagctttcc atatagtatt
     9721 tgatacggcc gacagcagct tcctgagcct gttttttctg ctcgctccgc atttgcagct
     9781 tctgagcggc ctcggcggcc agcttggctt tttcctccgg agacaggctg tatccctttg
     9841 ccttccactt aacctccgct cccgtccggt tgtttttgat gtagcctgcc gggtggccgt
     9901 ccaaatgacc aacataaaag ccgctgcgct cgcctttttt gtcgccgacg gtttcgattc
     9961 ggtgtttttg tccgtccata aacggatgcc cattagccac cacacacccc atttcgcgca
    10021 acttgtcggc aaactcttcc accggcgaca tcgcgggcat gctttgagtc tgttgctttt
    10081 ccggcaacca ttttgacgcg gccggtaaat tagcgttact gctttccata aaccaagatt
    10141 tgttggcttt gtcccatttg gcaccaaggg ctttagcctc ttctttttct tcatacggaa
    10201 cggcaagata aacacgggat gcgttttctt ctgccggtgc ttcttctgtt ttggcagcct
    10261 gatactcttg ctgtatggct gcgacctgac actcaactgc ttcttcctga ggagtatcgt
    10321 ccggcacggc ctcttctgcc aacgcttcct gcttcggctg catccaccgt gcaaacagtg
    10381 cggtattcac gccttcgggg atataccaag acttctgatc cttatcccat ttcgcgccca
    10441 atgcccgcgc ttcgtttttt tcacgaaacg gcacatcaat tagcgttttc gctatggccg
    10501 gagtattgtc cgttaccgtt tttgcttggt tggtggcact gtccagccat tttctgaaag
    10561 gctcttgatt gaccccggga gggatgtacc acgatttctc ctccttatcc catttcgcgc
    10621 ccagcatttt tgcctgttcc ctgtcggcat aaggaatttg aacgtaaacg cgctcatcgg
    10681 aaaccacgcg gacgctttcc ttgtcctgct ccgacagaaa ctggcggatc tgttcttcaa
    10741 ccgtacgata agaggactgc tcgatggctt cgtccaaagc ctctttggcc gcattcagat
    10801 tttccataaa tgactctcca ttctccggtt tttgatgccg ctgccaaaaa gcgttctgaa
    10861 acgtggcatc atccatctcc gaaataacca gactgaaacg cttcaagccc tcgtgcattt
    10921 tctcttcagc catgaagttg atgatcgccg ccacatcatt tttatcaacc gcattgataa
    10981 ggttcttttc ttccgccaag ctggcttcat caatattctg tctatcagaa atactcactg
    11041 ccgcctcgtt ctgctgttcg atttcccgca cctgatcaaa ggccatgaca tagccttgga
    11101 ttttttcggc gtcagaagcc gcacgcatga tttccatcgg atcgtcctgc aataccataa
    11161 tccagcttgc tacataactg gcatgttgcc cgggattgaa tgaaacgccc aattcctgac
    11221 tcagcatcaa tgaagcgatt tcggcacgca attcctcccg tgcgtaaccc tcgctgccga
    11281 acgggtggga aaggtcgcgg ttcaaacggt cagcatgccc cgtccaatgc cccaattcat
    11341 gcagggctgt ttcataatac gctcccggac tttcaaactg ctcgcgcagc ggcagggtaa
    11401 tactgtccct gctcggagag taaaacgccc tgtcgccgtg ccggtggtcg atgaccgcac
    11461 cagaattttt cagaatgttt tctgcccgct catccggatt ccaacttagc ggccgcagct
    11521 tcaacggcgg caagccgtct atctgttcgc cgttaaatac caccgcatga aagacctttg
    11581 gacgctccaa acgaaccgtt tccttctccg gtttgccgtc ctggccgata atttcctgac
    11641 ccttttcatc ggttttggga acttcctcgg tgaactgcca atattcgaca acagtaccct
    11701 tctctccacg gcgcacctgc gccccgatgg atgatgcctg tttataggtt atccagcgcg
    11761 gatcctttct gccttgtgcc tgcaaccaca gattgttaat gccgcgataa cggcggccgg
    11821 taatggcatt gtgcggcata aaatccggca acccgccgcc gtcccaaggt ttctgccacg
    11881 gcgcggttcc ctgccgcaac tgttcgatca gtttttccgc cacctgttcg taaaacggtt
    11941 ttttattctg catccctacg ctccttccgc cacttcgcgg gaaaggtctg ccacggaatc
    12001 ccgcgcggtt tccgcatcca ttgcggtctc ctcaaacgcc cccaaggctt ctgcctctgc
    12061 cggatcaaaa tccacttcca ttcccgggtt attcaaatcc gccacttttt ctgcgatcac
    12121 ttcgatgtcg atacttatac cttatccttt cgtatgtttg gaatagtccg gcttaccgcc
    12181 agcatcgggg taatactgct tgcactcggg gtaattgctg catccccaaa aatgcgttcc
    12241 tttctgtttt cccggccgtc tgaccaaggg atgggtgcag gccatgcact tgaattttga
    12301 cgacggctcg gcttttttcg gtgccagtac cggccgaccg gccttgtcgg aaaaagaaac
    12361 cttgcatccg tccttatagc cggtacagcc ccaaaaaaat cctttcgatc cgcgcatccg
    12421 ccttaaaggt ttgccacatt cgggacacgg gtacttcttg acattgagac tgatgccttc
    12481 ccgccctacc cgctccacct cgccggatat gtactccatc agactgttca caaaacgttg
    12541 cgcgtccaaa ccgccttgga caatatgctg ttgctgctca tgccataagg ctgtcatatc
    12601 ggggaacttc gcctgatcgg gcagcgcgtc ataaagctgt tggccgattt cggaactgac
    12661 aatgtttttg cctttttcca gcagaaaacc cttgtccagc agattacgca gaatttcgtc
    12721 ccgagtggcc ggggtgccga ttccgccgtg ctctccttcc ttacccttgt ctttctcaat
    12781 cagcagttcg cgcaatttcg gattctgcac atacttggct acccgtgtta aatcgttcaa
    12841 cagcgtatcc atcgtgtata acgggcgcgg tttggtttca aactgcgtct cttctgctgt
    12901 caaacaccgg ccgctgtttc ccggtttgag catctgcaaa tcaacactca aatcctcggg
    12961 atctgtcgcg gtttccgcat tgccctgatc gttcttgtat agagccatcc aaccgggctt
    13021 tacggttacg ttgctgcggc agacaaattt tttaccggca atttctgctt caatgtcggt
    13081 ttggttatat tcgtgatccg gccaaaactg ggcgatatag gcgcgggcaa tcagcagata
    13141 gacccgtttt tcgtcttcgg tcaggtcgtc catattggcc gttttgacgg taggaataat
    13201 ggcatggtgc gccgaaactt tatccgggtt gaacgccctg cttttgattt tcgtgtcggc
    13261 cgatgcaatg gcatttttaa agactgatgc ggtttttccg acagcatcca gcacgtctgc
    13321 cgcatcatcg aaatgctcgt cattgagata ctggcagtcg ctgcggtggt aggtaatcaa
    13381 acggtgtttg tctttcaatg actgtccgat gtcttttacc tgatcgggtt tcaaaccgaa
    13441 tttgcgcgaa caatccgcct gtagtttgag cagattgtat ggcagcggcg gactttgccg
    13501 cttggcagaa gttttagccg ctaaaatttt taccggctga ccactggctg cttcggctat
    13561 ctgccgtgca aacagcggat cggacaaacg tcccttatcg tctatcggat cttgatcctt
    13621 gtttggaacg taacgggcag taaaccggac accatccaca tccaaatcgg catacacatg
    13681 gtaataatgg gttttgacat gggactggtt ttcccgatcg cgccgcacca ctaaacccaa
    13741 aatcggagtc tgtaccctgc cgacggacaa cactccttga taaccctgtt ccgcagcctt
    13801 cagcgtatag gctcgggtca tgttgtatcc gtataactgg tctgccaccg atcgcgcttc
    13861 cgccgattgg gacaatcccc tgaatttggc gttgtcttct attgccgata acgctctctt
    13921 tacagatcgc tcgttattgt cgttaatcag tacccgtttg accggtttgg cacaattcaa
    13981 aaactccaac agttcgtcaa ccaacaactg tccttcgtca tcgggatctc cggcgtgaac
    14041 aatctcggta cattccgcca gcaaatcacg aataaccccg atctgttctt ttttgtcgga
    14101 caagggtttt ttacgccaag ggatatgcgc gatcggtaaa tcctcaaact tccagttacc
    14161 gtatttgctg tcgtaatctt ccggatcgca caaagcaagc atatggccgt aacaccaagt
    14221 gatgttgtct ccgttactgc aacggaaata cccgttgcgc ctctcgatac caactcccaa
    14281 agcggcggca atggctctgg ccacttcggg tttttctgca atataaagtc gggacatacc
    14341 tactcctcta tccatatcgg ttttacgaca tcacggatat gctctctccg gactatgccg
    14401 taataacgcg cgtcaaacga attggccacc gcattaccaa tcaaaatgta ctcatcgtct
    14461 ttcagacggc cttccccttc aaataccggt aagggctgcc ctagcccgtc ttgccataaa
    14521 ggccgggtgc cgccaagcag ccgtccgtta atcaacaacc cttcatcggt aaaacggtaa
    14581 ttgtcgccgg gaataccggc aacaaacttc atcaacccgg aataattttc cggacaaaaa
    14641 cccgctctga tataccccct ccttcttgcc attcggaaaa cttcggtatc gggagggcaa
    14701 aatgtaatgt agcttccgcg ccgtaccgct tcgccggata tgcggtaaac ccctttggcc
    14761 acactgcggg tatcgttgat acggaaaccg gctgcatata acagcccgta gcctgttacc
    14821 agcaatgcgg cggtcagaca catggcaagc atggcacggt caaacagcct tctcatagag
    14881 tgtcaaacgc gctgggcgta ttggcagtct ttttctgagt ccggtcggta tggcacggag
    14941 gcgggacttt ggagcgcgcc tcaaataccg gatctttgaa atacagggtc tgccgcccgt
    15001 aaatcgccgg atacccggca acataaatga ccatatcgcc tgcctgagtg atcaaacccc
    15061 ttgcgtcctt gaccggcccg ggcatacgca tacattcgtc ggctgtcagc aacggccgct
    15121 ggacttcctg cgtagtccgg gaaacctgcc ccaacatcgc acttacccgt ttgccgctgg
    15181 ttgtgatctg ctctttgaca accgttgtga tgccggtcag cttggacagg tgttccgccg
    15241 tctcgatccg gttaggcgga taagcgttct gcacatggca gctggacgta atggtttcat
    15301 ccgggccata ccccgtttcc cggcttttca gttggttcaa gtcttggcag atcaaataac
    15361 atttgatgcc gtaaccggcg ataaacgcca aggattcctg catgatttcc aacttgccca
    15421 aactcgggaa ctcgtccagc atggccagca tcttgtgttt gtagtggact ttcggcctcc
    15481 cgttttcaaa atccatcttg tcggccgaca gacggataat catattgatc agaatccgaa
    15541 ccaaaggccg caaccgcgcc ttatcgttgg gctgggtaat gatgtacaga ctgaccggat
    15601 cttcgtcgtt catcagatcc ttgattttga aatcggaacg actgacattg gcggcgacca
    15661 cattgtcgcg gtacaaagcg aggaaagatt tggctgtgga aagaaccgat ccggcctcct
    15721 cttccggtct gtccagcata tcctgtccgg cagaagaaat gaccggcaga ggacggccgt
    15781 ctgcatgggg atacgtcgcc atttccaccc ataaatcgcc taccggtttc tccggatcag
    15841 acagcatgcg gtcaacgctt ggtaaggtgg caaccccgcc atcatcccta gctttgtaaa
    15901 gcgcatgcag aatgacgccg gtcagcaaag cgaaagctgt tttgtcccag tggctgttca
    15961 gccccttgcc gtccggatcg acaatcattg tggccagatt ctgaacatcg cctatctcat
    16021 actccgtccc caccctgatc tcatccaaag gattccatgc cgcgctgcct tgcgcggaag
    16081 ccggttcaaa gcggattacc ttgttgtgcg cgtatttctt ccgccacccg gccgtcaatg
    16141 cccataattc gcccttcaaa tcggtaatga acgtactgtg cggccatgac agcaaagtcg
    16201 gcaataccaa acccaaaccc ttaccggatc gggttggggc ataagtcagg acatgctccg
    16261 gcccggaatg gcgcagataa tgcagggtac cctttttatc gacccagcca ccgacatata
    16321 caccgtcgct gtcctctcct tttttggcca gcagaccggc cgcctcgatg tctttcctgt
    16381 ccgcccaacg cgccgacccg tgcagagact catgcgcctt attggtattc ttccgcacaa
    16441 tattccatac caacagccct atcatgccga cggctaccac catcaacatg atgctgaatg
    16501 ccgtcataaa ctgctgctcg taccgtccgt gccatttacc gtaccaaatc agaatgctcc
    16561 acggcatata gaacttgccg atgccgtatc ccaactggga atggtattgg aactgatggg
    16621 caaaatattg tgtggctgcc cccataccag ccaacagcac caataccaga caaataaagt
    16681 ttaccaaacg gccgcttcct gtcgcttggg cttggttctg atggccgacc gcattgttgc
    16741 gtttgttctt gctgcgaaac atgtttatcc tttgaaattt aacgatgcct tcccttaaca
    16801 ggtctgatct gccccgtcgg agtaactttc acttcactgc ccagtttcac gctcgaaagg
    16861 cgtctggaca catagggtgt aacggacatg accagtattt cctcgacctc tttcggctgg
    16921 aataaagcca gtgcctgccc gtctatgttg cggatgcccc tgaacaacat cacaccggaa
    16981 tcacttggat tatacttcct atgattcaat atatcgaatt ttttgtcaat tttctcctgc
    17041 cgctcggcaa tatatttgtc gataggtgcc aatgaatgag taagtctctc gttttcttgt
    17101 gcaggctcag gatttttcgg aatctctact accgcagcgg atgaaatttt gatttcagcc
    17161 aacccttcca tcccccgcaa actgccccgc ctgaatcccg gcggtttttt tcccggtttg
    17221 gcaaccaaac cgacaactgc ctgcatctgc ctgatctcag ctactcctcc tgtccgatgt
    17281 attgtccgtc gcacagcctg atagtttgcg gatccttgct gctccaagtg actagaaaca
    17341 tcacgcggca atagcacttg agttcgtcct tggatgcgaa ccagactgcg ttcgggcatt
    17401 cctcgcagac tgtcgcttcc tgcggaagca gactgccctc ttcctgctcc aatgcctcca
    17461 gagtcggaga gcttcgggat tgggtatcct gatatgcctg catggaagcc tcgatttcgg
    17521 cttgcgtcat acccatgcca tcgatagcgt ctaggctttc ctcctgcata tcccgcgcct
    17581 cctcgttgct gccctgcccg cccatctgcg cggccagttc catcagcttg tctgtatcga
    17641 tcggtacgtt ccctgacata ttcgtattcc ttcacatact gttgataaag atggttcaga
    17701 cggccgtctg taaacgacac atccaaaccc tttgcggcgg caattctggc caccttatcc
    17761 tgaaaatgcc gcgttccgcc gatagtcagg acttttccgt atcgccctat tgcgatttcc
    17821 aacgcccttg ataccgcctt atcgtcagga atcgcggcca gtttgagcac actgcccgta
    17881 tcacggatgg tcatgccgtc ctcggcataa ataaccgtcc cctttttcgt taccgtatca
    17941 accttgatcc cggcaaatcc gccactttct ttgccgacaa agcggttgcc cttgtaacct
    18001 gcatccttcc ccctccttct caacgcatct aacgcccgga catcgccttc ggcagcacgg
    18061 tgtttcagcc aatctgccca agcaaaaccc ttgttctgct caaaaaaaac cgcacgtcgg
    18121 cgggcataat cggttcttac cgatctgatt tcatccttca tttgcctaaa cagcatccga
    18181 tacagaatgc gccttgcaat accactgctg ctatgcctaa tcaggctgcg gcgaaaagcg
    18241 tatttttctt tgatgcggcc gatctccata tcccggtttt gtgccaaatg caggaaaaaa
    18301 tcctgccgcc tgcttttctg aagctctttt tcctccttgt attgccggaa cagatcgtcc
    18361 acgttggctt tgctcaacgg tttctcacgg tacgttttgg atggtttcgc ctttactcct
    18421 gttttctgat caaatgcacc aagccttttt tccaacgccg acttggaaaa ctccctatct
    18481 accgaacttg ccttgacatg atgctcgccg tcgctgataa caaaaccgtt acccctcagc
    18541 ttgatttcca atccgtgatc actcagtaca tggtacagtt gctcccatgt cttagcctct
    18601 ttaatctgct caccgcactc tctcctgatc catcctatca ggctctcgat acccgccact
    18661 tcctccatat cgattttttt attgccgcgc ccgccacgct gcaccctctg caaaccgtac
    18721 tcttcttcaa acttcctgca ctcccgccct aaaattttga agtcgttata cggctcgtgg
    18781 atggtctttt tttctgggtg cactttgtta atcgctacat gaaaatgcag attgtcggta
    18841 tctctgtgaa caacagagat ccgctgatgt tcggcatacc ccaaagcctc aatcatgctc
    18901 tgctcaatac ggctcaactg ttcgtccgtc aaaaattccc cgcccgcaag ggataccatc
    18961 agatgatagg ttttatccgc cgcactgcgc ttattctgat cctgaactgc caaagtctcc
    19021 tgaaccgccc acctcaaatc cgatgattca tagttatgaa tctccacctt tcccactcgg
    19081 tttgcgacac cttgagaacc ggccagatag gtaactaagc cttcaaaact gctcaggttg
    19141 gctttacgca tcggaatttt ttttgcaatc atgttcagcc tattttttta gccgctaaaa
    19201 ttctgcggct ccgcacaatc tccaacatac tttcctgcat ttctccaatt tgctccaaca
    19261 ctccgaagat cgccttagcc aactgatcgt gatcgaacat catcagccgc tcgtcgttgg
    19321 ccaaccacat cttgatcaag ccgcccaatc ggccttggtc ggcattgatt ttggccaagg
    19381 ctttaaccgc ttcaccgtca taaacggatg caggcttgta tcccaagcca agtaatttca
    19441 aataagccga ttggctcaat ccgacagatt tagccaaact ctctatggtt ttccattctt
    19501 cttccgaaac gtaaacgctg atgtattttg gccgttttcg tgcgcctgcc attcttggag
    19561 tccttaattt gtgccgcaag gcacacaaca gccaacggta gttggccagc ctcgcagagc
    19621 aagataatcc gttgagccta ccggcgaata aggattgtga tgtacatgta catcacatat
    19681 cttgccccaa ttctggggtt caatacgcca aggattatac gccaaataat tcaatatatc
    19741 gaacatattt tgatgacaaa cctcctaaaa atgctttatc acaaacaaaa aatgatttac
    19801 tatatcgaat tattgttttt aaaacgattt tgcttggcag ttttggcaag gctttgtcaa
    19861 aaaattggca aagccctatc gaaatgatcc aaatgggaaa aatagaaaaa ctggttttgg
    19921 ccgaacttgc cgccatgcag aaaaaaggct cctcagccgg aaacaaggca aggttcttgg
    19981 ccatgcagga agacattaag atactgctgg tggacaggaa tatccccgtt atcagtgttt
    20041 ggcggatttt gtcggaaaaa aaatatgtcc catattccta tgagacattt aaccgctact
    20101 gcaagatgta tatcttcaaa tccgacacta cggccgctga ccaaacagac accgcccagt
    20161 ccacacagga aaaacctgat tccatccgca cggacaaaga accgccgtct cccgcctccg
    20221 aacccattat cgcgggaggc aacaccaaaa gctcttcagg ctttactttc aacaacaacc
    20281 cagacggaaa ggacttattt taaatggcca ttatccatat cagcctccaa gcaaaagggg
    20341 gcgtcggtaa aagtttcttg gctttgatgc tgtctatgtt ttaccgccat atcgaaagac
    20401 cggtaaaaat tttcgatacc gatccggcca acgcaaccct gttcggtttt cccgcactgg
    20461 aggcggaaaa attcgagatc atgaaaaagg atgatattga caaacggttg tttgaccggc
    20521 ttttctcaca aatcctgtct gcaaccgatg atgtcatcat cgatgttggc gccaatgcgt
    20581 ttattccgct ttgttcgtat atcatttcca acaatctgat taccatgctg acgcaggaag
    20641 gccatcaggt cataatccac acttccattg tcggcggcca gaacctgaag catacggtca
    20701 acggatttga tgccctatgc tcgcaatttc cggaatcaac cggtttcgtc atttggctta
    20761 atccgttttg gggggaaatt gagtttgaag gtaagtcgtt tgagaaattg aaagtctatg
    20821 agaaacacaa atcccggata cgttccatca ttaagattcc ggaatgggac gcttctctgc
    20881 acggccgcga ccttgccgcc atgctggaaa agcaaaaaac attcgacgaa gccttagcgt
    20941 ccgaagattt cagcatcttt gaacgccaac gtttgaacat tatcaaaaac gaaatgttcc
    21001 aaacgataga aaccgcccaa atcgtctgag tacatcatgg atgaaaaagt aaaagccata
    21061 ttagccacca ttgttgaaaa aaacggcatc gccttatctc ccaaagaccc gataatggtt
    21121 gtattcactg ccttagaata tatgctctcc gaacagcacg cagagcaaaa ggctctaacc
    21181 gtcttattca aagcgcaggt agaagaggcc acgggaaact ggatacagtc cgttcaccaa
    21241 cagactgagg aacttgtcag ccagcaactc aatacgacca aagaacaggc aaaggaaatg
    21301 tccaaattac tgcttcatga gacagacagg ctgcttgagc agagaattac tgatgccgta
    21361 aaagactttg aaggaaagct atacggcatc aacgacaacg ccatgaatgc agtagaattg
    21421 agaataaagg aaactcggaa cacggcaatt ctcaatctga cggccgccct gattgtcttg
    21481 ttagcagtca ttatcttggc gttcaaattt accttctgac aaaaaacccg gcaatgccgg
    21541 gttttttgtc tccattcttt acgccaagag aaaaatcagc gtactatttt ttgacctagg
    21601 gacaggctta atccagcttg ccgtttctgt tgttcttttt ctttgcgctg tttttcatgc
    21661 agattgtgaa ccctgacagc cgtttgaaca tcatcaaatt tttttgccag atccggttcc
    21721 tccatcctta acttcctagc agccaactcc tgtattttcg gaacacccag agccatcccg
    21781 tcgcgaatct cgcgaacatc ttccaacctg tcatgcagac ggttcagcct tgattgtgcc
    21841 actgcaatgt ttgcactcca cgtctgtttt ttggacggca tgctcaacat tcccggccgg
    21901 gcgttttgca atgcctgcaa cttggccatt tgcccggaaa tcaattgctc caatttttct
    21961 tctatgcgct caacctgttc tgtcttttcc aagacataag tatttaacac accggcgaaa
    22021 gtttcctcaa taacctgcct gctttcaaac aactcttctt gttcggtttc agccgcaaca
    22081 gctttagcta caagcccttt atcttcattc atggtgttat cctaaaattt tagcggctaa
    22141 aatttcattt agcgcgttaa agtttttttc tctccgatac ccgccttttc tactgttgcc
    22201 gctttgtcct tgacactgat tgagaccact tctccgatcg acggaacgga agaaaataga
    22261 gaacacgggt atttgactat agactgtcgt gttttctgat aaacatagct gtcattttga
    22321 aaaacgattt gcccttcgta cctgacagaa tcctgaggag atttggccgt atgcagcaaa
    22381 taaattcccg gcttcatatt ggcaggagca ggcttaaccg cctgcgtaat ccatttgccg
    22441 tctttttctg tctcgaacaa atgctgtttg ttcaatacca gcactctttg tttttctgct
    22501 tccattctgc taaaaatttt caaaaaagat gtgaataatt ctaacagaac agtctttaat
    22561 ataccccccc cc
//
